LOCUS       DAVHEW010000001        79051 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVHEW010000001 DAVHEW010000000
VERSION     DAVHEW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 79051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.001"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(247..2898)
                     /gene="adhE"
                     /locus_tag="VVL53_000001"
                     /gene_synonym="adhC"
     CDS             complement(247..2898)
                     /gene="adhE"
                     /locus_tag="VVL53_000001"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU4182239.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELSFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(3198..3608)
                     /locus_tag="VVL53_000002"
     CDS             complement(3198..3608)
                     /locus_tag="VVL53_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182240.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            complement(3610..4038)
                     /locus_tag="VVL53_000003"
     CDS             complement(3610..4038)
                     /locus_tag="VVL53_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU4182241.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            complement(4084..4383)
                     /gene="yajC"
                     /locus_tag="VVL53_000004"
     CDS             complement(4084..4383)
                     /gene="yajC"
                     /locus_tag="VVL53_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU4182242.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            complement(4500..6476)
                     /gene="tkt"
                     /locus_tag="VVL53_000005"
     CDS             complement(4500..6476)
                     /gene="tkt"
                     /locus_tag="VVL53_000005"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU4182243.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            complement(6590..7681)
                     /gene="ulaG"
                     /locus_tag="VVL53_000006"
     CDS             complement(6590..7681)
                     /gene="ulaG"
                     /locus_tag="VVL53_000006"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU4182244.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNSKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            complement(7793..9466)
                     /locus_tag="VVL53_000007"
     CDS             complement(7793..9466)
                     /locus_tag="VVL53_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU4182245.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            complement(9643..10347)
                     /locus_tag="VVL53_000008"
     CDS             complement(9643..10347)
                     /locus_tag="VVL53_000008"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU4182246.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            complement(10349..11212)
                     /locus_tag="VVL53_000009"
     CDS             complement(10349..11212)
                     /locus_tag="VVL53_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU4182247.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            complement(11216..11881)
                     /locus_tag="VVL53_000010"
     CDS             complement(11216..11881)
                     /locus_tag="VVL53_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU4182248.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGIDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            complement(11898..12383)
                     /locus_tag="VVL53_000011"
     CDS             complement(11898..12383)
                     /locus_tag="VVL53_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4182249.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            complement(12459..12740)
                     /locus_tag="VVL53_000012"
     CDS             complement(12459..12740)
                     /locus_tag="VVL53_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4182250.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            complement(12763..14220)
                     /locus_tag="VVL53_000013"
     CDS             complement(12763..14220)
                     /locus_tag="VVL53_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU4182251.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            complement(14351..14974)
                     /locus_tag="VVL53_000014"
     CDS             complement(14351..14974)
                     /locus_tag="VVL53_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU4182252.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKVFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            complement(15025..16011)
                     /locus_tag="VVL53_000015"
     CDS             complement(15025..16011)
                     /locus_tag="VVL53_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU4182253.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            complement(16030..16854)
                     /locus_tag="VVL53_000016"
     CDS             complement(16030..16854)
                     /locus_tag="VVL53_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4182254.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            complement(16829..17200)
                     /gene="rnpA"
                     /locus_tag="VVL53_000017"
     CDS             complement(16829..17200)
                     /gene="rnpA"
                     /locus_tag="VVL53_000017"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU4182255.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNRFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            complement(17349..18539)
                     /locus_tag="VVL53_000018"
     CDS             complement(17349..18539)
                     /locus_tag="VVL53_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU4182256.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            complement(18590..19543)
                     /locus_tag="VVL53_000019"
     CDS             complement(18590..19543)
                     /locus_tag="VVL53_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4182257.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKENNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            complement(19604..20198)
                     /locus_tag="VVL53_000020"
                     /pseudo
     CDS             complement(19604..20198)
                     /locus_tag="VVL53_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(20335..20748)
                     /locus_tag="VVL53_000021"
     CDS             complement(20335..20748)
                     /locus_tag="VVL53_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU4182258.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            complement(20726..21187)
                     /locus_tag="VVL53_000022"
     CDS             complement(20726..21187)
                     /locus_tag="VVL53_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU4182259.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS"
     gene            complement(21150..21410)
                     /locus_tag="VVL53_000023"
     CDS             complement(21150..21410)
                     /locus_tag="VVL53_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182260.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(21415..21819)
                     /locus_tag="VVL53_000024"
     CDS             complement(21415..21819)
                     /locus_tag="VVL53_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4182261.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFSAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(21812..22078)
                     /locus_tag="VVL53_000025"
     CDS             complement(21812..22078)
                     /locus_tag="VVL53_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEU4182262.1"
                     /translation="MLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAEL
                     YSLEKNEDASLRKLQADGRITEEQAKAYKEYNDKNGGANRKVND"
     gene            complement(22080..22337)
                     /locus_tag="VVL53_000026"
     CDS             complement(22080..22337)
                     /locus_tag="VVL53_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182263.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYQVPILKNEYTVKLLLLME
                     CRIEDQEVIEYLVSTWKYRDLVLHSVRMYEIEKSESFTILD"
     gene            complement(22608..23564)
                     /locus_tag="VVL53_000027"
     CDS             complement(22608..23564)
                     /locus_tag="VVL53_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU4182264.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKHTDGNWYYFDQSGEMATGWKKIADKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(23568..23900)
                     /locus_tag="VVL53_000028"
     CDS             complement(23568..23900)
                     /locus_tag="VVL53_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU4182265.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(23904..24203)
                     /locus_tag="VVL53_000029"
     CDS             complement(23904..24203)
                     /locus_tag="VVL53_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182266.1"
                     /translation="MPIEEVEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(24212..24562)
                     /locus_tag="VVL53_000030"
     CDS             complement(24212..24562)
                     /locus_tag="VVL53_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182267.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            complement(24565..24768)
                     /locus_tag="VVL53_000031"
     CDS             complement(24565..24768)
                     /locus_tag="VVL53_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182268.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(24863..30409)
                     /locus_tag="VVL53_000032"
     CDS             complement(24863..30409)
                     /locus_tag="VVL53_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEU4182269.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGEYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMTTGRSLELDNVFITQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFIFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGFEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQVEALSRTKNELDGASTLLAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEA
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASRIASVQA
                     SGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGYNALKIIGLNPSGRDGGNPK
                     VTYPVLGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIK
                     RYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQI
                     STVESTFKQRVNSLEAGVNRLTEGLRTKVDISALNVTAENIRQSVKSLETDTQNKLNQ
                     KLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKT
                     VGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTFSAWIKYENVVQG
                     RNFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITFTYDYSEKTNFDQ
                     LKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDGENELLVAKTEFKRTADGL
                     STKMAAVESYVGQDGQRQEALQRYTREESARQATAVREQVNRDFVGKATYQEDVKGIN
                     QRIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSE
                     QGTTTQISNISNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQVSANKANAD
                     SQFANVTNQLARKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEV
                     AKNASNGQNLLKGTKDFSGGWKNKGANWKKHAEKYKGVDVLFKNNSWNGVGQEIDAKI
                     GEVYTFSLWMKSDWKNDTVNFYVNRNGSVEKGWGVPSETSVAITSEWKRYSFTFKITV
                     DGFIFPRVERLNQNTNLYIAGLKLEKGSYATPYTEAPEDTDEAIRSVQSQLTGSWAVQ
                     NINSAGDIISGINLGANGHNRFVGKLTHITGETLIDRAVIKSAMVDKLKTGNFEAGSV
                     TTTILDAEAVTAEKLKVDNALIRKLTATDAFIYELISKRIFSTKVESVISSSTFLEAY
                     QGRIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAGYGVRTAFWANWGNNWNFAGPKAW
                     NVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTTFSRSPVFSNGIELGSKDVLGDGW
                     NPKGGRNAVVWWNQVGSGSLKYWMEQKSDRRLKENITDTAVKALDKINRLRMVAFDFI
                     ENKKHEEIGLIAQEAETIVPRIVSRDPENPDGYLHIDYTALVPYLIKAIQELNQKIEK
                     MEKTIA"
     gene            complement(30414..30764)
                     /locus_tag="VVL53_000033"
     CDS             complement(30414..30764)
                     /locus_tag="VVL53_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182270.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSKFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     LKPWSGAKFNLVER"
     gene            complement(30773..34426)
                     /locus_tag="VVL53_000034"
     CDS             complement(30773..34426)
                     /locus_tag="VVL53_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182271.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSSSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIERIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNIGNISEAVGNIAQDFSSTFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPILALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDIFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSWFGN
                     MFTSAYNAVKNAFSSIGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFKSAVNAVLGTIE
                     NVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGISPQGGFSSDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            complement(34413..34763)
                     /locus_tag="VVL53_000035"
     CDS             complement(34413..34763)
                     /locus_tag="VVL53_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182272.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            complement(34802..35182)
                     /locus_tag="VVL53_000036"
     CDS             complement(34802..35182)
                     /locus_tag="VVL53_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182273.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVDK"
     gene            complement(35187..35600)
                     /locus_tag="VVL53_000037"
     CDS             complement(35187..35600)
                     /locus_tag="VVL53_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4182274.1"
                     /translation="MLANGITLSYGETKDSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKKKLYFEQAYPDGTKVIFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSNLTFTDGIGG"
     gene            complement(35603..35971)
                     /locus_tag="VVL53_000038"
     CDS             complement(35603..35971)
                     /locus_tag="VVL53_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182275.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGIVDLHSELVFQFRMEN"
     gene            complement(35968..36483)
                     /locus_tag="VVL53_000039"
     CDS             complement(35968..36483)
                     /locus_tag="VVL53_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU4182276.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            complement(36458..36796)
                     /locus_tag="VVL53_000040"
     CDS             complement(36458..36796)
                     /locus_tag="VVL53_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182277.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            complement(36777..37088)
                     /locus_tag="VVL53_000041"
     CDS             complement(36777..37088)
                     /locus_tag="VVL53_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU4182278.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTDLLQRIRMHRLARVAGHVFEA
                     E"
     gene            complement(37090..37278)
                     /locus_tag="VVL53_000042"
     CDS             complement(37090..37278)
                     /locus_tag="VVL53_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182279.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVA
                     ISDVLEEIFPKPEFLEED"
     gene            complement(37288..38313)
                     /locus_tag="VVL53_000043"
     CDS             complement(37288..38313)
                     /locus_tag="VVL53_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEU4182280.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAGANNAYNAVLDVSEKLDEL
                     GITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKPSTQGKVVNRAKAWKTGTTYKEGDTVTHADKVYVAVKDISNSSTAPDTDTTNW
                     KEKTGKK"
     gene            complement(38336..38845)
                     /locus_tag="VVL53_000044"
     CDS             complement(38336..38845)
                     /locus_tag="VVL53_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU4182281.1"
                     /translation="MNEETQTVETVEEQKVPAEPAQQPQDEKKYTDADVDAIIDKKFA
                     KWKSEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAKAMLSE
                     RDLPVELVNVVDLTSAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQTVQQEV
                     TEKWRKDFL"
     gene            complement(38991..39203)
                     /locus_tag="VVL53_000045"
     CDS             complement(38991..39203)
                     /locus_tag="VVL53_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182282.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDL
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            complement(39345..39632)
                     /locus_tag="VVL53_000046"
     CDS             complement(39345..39632)
                     /locus_tag="VVL53_000046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182283.1"
                     /translation="MNKYKKLIELIENNGLEIQSKKCYDPQSAWHGEELWIVDKKKQN
                     KIFDLSGNGYCFHDAKVEEAIEEVEKYLLLKKMDTFDDFKKWVEKNAKPKK"
     gene            complement(39675..40088)
                     /locus_tag="VVL53_000047"
     CDS             complement(39675..40088)
                     /locus_tag="VVL53_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4182284.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            complement(40085..40294)
                     /locus_tag="VVL53_000048"
     CDS             complement(40085..40294)
                     /locus_tag="VVL53_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182285.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            complement(40296..41933)
                     /locus_tag="VVL53_000049"
     CDS             complement(40296..41933)
                     /locus_tag="VVL53_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEU4182286.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPESEIKHLQSFSWVGDGSTYSTNIWKNTGKLTSSIKDELLISLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            complement(41842..43311)
                     /locus_tag="VVL53_000050"
     CDS             complement(41842..43311)
                     /locus_tag="VVL53_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU4182287.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILKHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGLTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKYQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            complement(43323..44537)
                     /locus_tag="VVL53_000051"
     CDS             complement(43323..44537)
                     /locus_tag="VVL53_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU4182288.1"
                     /translation="MTNKFTKRQEEVLTRVLNDDFFICGLHGAKRSGKTVLNNMVFMN
                     EIARVRETADRLNIDEPMYILAGTSSTSIQNNIIQELYNMFDIEPKYDKHGAFTLCGV
                     KVVQVYTGSISGLKRARGFTAFGAYVNEASLANEQVFKEIISRCSGEGARIVWDSNPD
                     IPTHWLRRDYINSGDDMIIDFHFKLDDNTFMSDRYRENIKNATPAGVFYDRDILGLWV
                     TGEGVVYRDFSENMVVDNVPKDITKVYAGVDWGYEHFGSIVVIGETSGGSAYLLEEHA
                     HQYKEIDFWVDLAKNIKERYGNITFWADSARPEHVARFQREQLRTFNANKAVLSGIEE
                     VAKLMKAGRFFVVSNKVSKFKDEVYQYIWNEKTGEPVKENDDVLDAVRYAIYSQHSQP
                     KATVRRRSDYGL"
     gene            complement(44527..45021)
                     /locus_tag="VVL53_000052"
     CDS             complement(44527..45021)
                     /locus_tag="VVL53_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU4182289.1"
                     /translation="MDIGLNQRQKMFASEYLRTGNVYQSAIFAGYSEAYAKTTASKLL
                     ENASVKTFIQTETEKMHDENILSAKEALSILSDIARGQRLEEVLMMNPVTGEVDRVTK
                     KADNNTVIKAIAEILKRYPTAKQAEKLELEIEKLKSQIGVDNEQDDKLIDFAKALRGA
                     FDDK"
     gene            complement(45482..45886)
                     /locus_tag="VVL53_000053"
     CDS             complement(45482..45886)
                     /locus_tag="VVL53_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU4182290.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWIDVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            complement(45958..46323)
                     /locus_tag="VVL53_000054"
     CDS             complement(45958..46323)
                     /locus_tag="VVL53_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182291.1"
                     /translation="MKPKRYPYSGKRQKVSSPLFPARPILNEVPIVEEVKVELGVEAK
                     VGRFYPETIIQLDISGYGNRVVSVHRFPGTLLSVGESIQLKMLFYKKLRNLTTDRFLT
                     FRESDWKFFIRDLVNEFKH"
     gene            complement(46320..46562)
                     /locus_tag="VVL53_000055"
     CDS             complement(46320..46562)
                     /locus_tag="VVL53_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182292.1"
                     /translation="MVDKYIDEPHTTFIPVINGKSSVLVPTRTKRKYILVVSEFTGNK
                     QVEETFEVTVEEYKHYKIGNTFIQDAVLENKEGDKQ"
     gene            complement(46637..47077)
                     /locus_tag="VVL53_000056"
     CDS             complement(46637..47077)
                     /locus_tag="VVL53_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182293.1"
                     /translation="MIPKFRVWVKIGKRMVFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYEKDYVG
                     VVKVLEGSWVIDTGSVAVRLWSEIDESEVLGNIYENLEFLEVNE"
     gene            complement(47074..47577)
                     /locus_tag="VVL53_000057"
     CDS             complement(47074..47577)
                     /locus_tag="VVL53_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU4182294.1"
                     /translation="MNIKALIKKYEELWNEHSPFYEPVPYTSMVELFLKELKQLDEPQ
                     KVKIPQFVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLDGYE
                     VEEEKRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEEAKFGWVFDCEGI
                     DIEEVEE"
     gene            complement(47579..47896)
                     /locus_tag="VVL53_000058"
     CDS             complement(47579..47896)
                     /locus_tag="VVL53_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182295.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQNLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     RGN"
     gene            complement(47921..48103)
                     /locus_tag="VVL53_000059"
     CDS             complement(47921..48103)
                     /locus_tag="VVL53_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182296.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            complement(48119..48550)
                     /locus_tag="VVL53_000060"
     CDS             complement(48119..48550)
                     /locus_tag="VVL53_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEU4182297.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRTRLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            complement(48547..48708)
                     /locus_tag="VVL53_000061"
     CDS             complement(48547..48708)
                     /locus_tag="VVL53_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182298.1"
                     /translation="MASKINVTERIAIIIEKQKIEVVTTLSYDMSIEFVNENRENLFE
                     MAGFKGALE"
     gene            complement(48722..48931)
                     /locus_tag="VVL53_000062"
     CDS             complement(48722..48931)
                     /locus_tag="VVL53_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182299.1"
                     /translation="MEWTDWVDWKPETKTDIKIKIENDGYTFPHYDKKNNGVKYVIST
                     MDIKQDCLRLGVPFEDVYPLQTTLF"
     gene            complement(48933..49629)
                     /locus_tag="VVL53_000063"
                     /pseudo
     CDS             complement(48933..49629)
                     /locus_tag="VVL53_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(49673..49918)
                     /gene="ssb"
                     /locus_tag="VVL53_000064"
                     /pseudo
     CDS             complement(49673..49918)
                     /gene="ssb"
                     /locus_tag="VVL53_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(50013..50348)
                     /locus_tag="VVL53_000065"
     CDS             complement(50013..50348)
                     /locus_tag="VVL53_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEU4182300.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            complement(50341..50664)
                     /locus_tag="VVL53_000066"
     CDS             complement(50341..50664)
                     /locus_tag="VVL53_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182301.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFKVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEKFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            complement(50667..50828)
                     /locus_tag="VVL53_000067"
     CDS             complement(50667..50828)
                     /locus_tag="VVL53_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182302.1"
                     /translation="MTEIEKISEELAEYGVPDELIGKIEDLLATLYGEKKEIGDRKIL
                     DISPESMGR"
     gene            complement(50831..51871)
                     /locus_tag="VVL53_000068"
     CDS             complement(50831..51871)
                     /locus_tag="VVL53_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEU4182303.1"
                     /translation="MQELQVKVTQAQVEIIDREKFEQNINEVVAKYQNYTVTAATIKD
                     DKQTLADLRKLDKQVSDERIRNKKVLSEPADEFDKYVKNAIQPLKDIITKIAGDVKEF
                     EEHQKAVRIDTVKGYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTLKKATMKSL
                     DDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFGQIKADYEFE
                     KQKEEIRQAQERAERESQELLAAQQTKQQEQAPKSTETPNFDPETGKILDGGQILQNE
                     PNALRGAENDLKRYAQKMTLEVYFVDTAEKDRFKAGLSQLGFDFKKNYQVRGYQRIEP
                     LTQAELNEQCGW"
     gene            complement(51881..52645)
                     /gene="bet"
                     /locus_tag="VVL53_000069"
     CDS             complement(51881..52645)
                     /gene="bet"
                     /locus_tag="VVL53_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEU4182304.1"
                     /translation="MTNNQLATQIKRDITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYQNNSGTEFSLIVSKEAFMKRAERCEGYDGFEAGITVM
                     RNGEMIEIEGSLKLPEDILIGGWAVVYRKDRSHRYKVTVDFNEYVKTDRNGNPRSTWK
                     SMPATMIRKTALVQTLREAFPDELGNMYTDIDGGDTFDAIKDVTPQESREDVVARKMA
                     QIDQFNKEQEANHADPEPTQNEDPIQGELLDGELEY"
     gene            complement(52657..52887)
                     /locus_tag="VVL53_000070"
     CDS             complement(52657..52887)
                     /locus_tag="VVL53_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182305.1"
                     /translation="MTQMTNKGKSFIRAEISEKQKEYIRLLAELRGVTTQELLGQVVE
                     RFIDRNLQLIQDYKNELDDLNSKSRRRIDMNT"
     gene            complement(53007..53150)
                     /locus_tag="VVL53_000071"
     CDS             complement(53007..53150)
                     /locus_tag="VVL53_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182306.1"
                     /translation="MTKIEIENRVWLLANHEEKNELLDLGLTSKARYVKRVLELGKVY
                     AHV"
     gene            complement(53137..53397)
                     /locus_tag="VVL53_000072"
     CDS             complement(53137..53397)
                     /locus_tag="VVL53_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182307.1"
                     /translation="MFKALKTIKKIKQLQKEMHDVSLAFLALQDVGLMPEDERSKAKA
                     QTMHDVSHMLKDVLGGKSVDEAMKRLNSEVKIEEVEQEDDKD"
     gene            complement(53390..53596)
                     /locus_tag="VVL53_000073"
     CDS             complement(53390..53596)
                     /locus_tag="VVL53_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182308.1"
                     /translation="MKSLTKLKLRLEGIIKEVSLDWRVVAVELNEDLLEERKRRFMCE
                     QEIYDLKQQLAIYKEKEQMGEQYV"
     gene            complement(53596..53757)
                     /locus_tag="VVL53_000074"
     CDS             complement(53596..53757)
                     /locus_tag="VVL53_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182309.1"
                     /translation="MKRKKEQWKPRIVNIMADGSVIEDLTGYVIPAGHSYYDIILGMN
                     KRSNKEDVA"
     gene            complement(53975..54829)
                     /locus_tag="VVL53_000075"
     CDS             complement(53975..54829)
                     /locus_tag="VVL53_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4182310.1"
                     /translation="MQVVSSKELQERALQVETLKQQCPKHEGVYMWRSINPCTRNTLT
                     YCPECVQETINQNASEQLAIAEAQIRDTRSYSLFMKESIIPNDLKNATVGNFEIHTNQ
                     DAKAVNFAKRVTADYVKERYEGNTIISGPPGVGKSHLAVGIAKTLNESFQMLQVRKSV
                     VYMPSMELFSRMQEAFQYKDSKWEQRSVVKFLQNVDFLILDDLGKESSVGNEIRQGNN
                     WMQKVLYQILENRTNTIITTNFEGKHLKELYEQSLVDRITKGNMKTNAFKFSKDTASR
                     RSLSATDY"
     gene            complement(54839..55690)
                     /locus_tag="VVL53_000076"
     CDS             complement(54839..55690)
                     /locus_tag="VVL53_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEU4182311.1"
                     /translation="MNEQYFTEIDMDNHERYFKIPYRLLEDDYFSDLDPLAVMVYGIL
                     TDRVSLSRKNKQHFTDKDGYLYVVATNEEIGKWIKKSEPVVIKLKKQLIEHGLLKEKR
                     QGVRLANLLYPQKIRTKEILVQELKNIKGGTKEILVQELKNIKSNQPDNNHPYITNLI
                     EPEGVGANNLYSIEDAPAENDLGIVHDWIFSEFGRYPTPFEIEDLKYFLQDHSKEVIK
                     LAIKECVGNGKPYFKYLESILRDWKQKGLTTVELVENRQKPTRSNNKSNGRLRLSDDG
                     FDPRLGF"
     gene            complement(55687..55914)
                     /locus_tag="VVL53_000077"
     CDS             complement(55687..55914)
                     /locus_tag="VVL53_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4182312.1"
                     /translation="MPKMTLRAIRTNYNLSAKEVADKLNIHQQTLLKYEHDSSKIPMD
                     LLDKLARLYNVEKDFIFLGKKYELNHSLGEV"
     gene            complement(56105..56395)
                     /locus_tag="VVL53_000078"
     CDS             complement(56105..56395)
                     /locus_tag="VVL53_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182313.1"
                     /translation="MTKTIAINTSEHDVLLTARKTHPAVFVDGMFLDGVERVEFTNRF
                     LESCEVVLTFNDRVETNPFPLNDITLLEKLFGKASNGQSLRDIVLQTLEDGN"
     gene            complement(56392..56538)
                     /locus_tag="VVL53_000079"
     CDS             complement(56392..56538)
                     /locus_tag="VVL53_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182314.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLLKQVSHEIELRALES
                     ETKV"
     gene            complement(57129..57404)
                     /locus_tag="VVL53_000080"
     CDS             complement(57129..57404)
                     /locus_tag="VVL53_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182315.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEEIFLYSKLEAFCTNCRQF
                     AFGWTELAEEIECQERYLLDSGFTQDEIDDIRFDAAFVGMQDKMNVA"
     gene            57579..58385
                     /locus_tag="VVL53_000081"
     CDS             57579..58385
                     /locus_tag="VVL53_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4182316.1"
                     /translation="MARGRGKLTPQDKEDMKVFSANLNSILSDRNCKQAELSRATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADFFGVPKSVLDPRFVTNNSMVDDSSSNTSSIQTIY
                     DQLEPPRQGKVLNYAKRQLKEQRNEEETKINEVSENIIRLDDYRQTTYRRVTGVVSAG
                     SGSIQDDDLDMEVSFYEDEIPDDYDAIAYVVGNSMEPKIKNGDYLFIKNTQQVDYNTI
                     GIFQVDGANYVKKLRQGYLESLNPDYEDIHLDESNDIRTIGEVVSVYREK"
     gene            58387..59151
                     /locus_tag="VVL53_000082"
     CDS             58387..59151
                     /locus_tag="VVL53_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEU4182317.1"
                     /translation="MSKENPYFEQTKQNYIEVEKLYKLGKAKHTSSKYRFLAPAVKRQ
                     SEQFLFEAKTQKRKYWKFSRGSLIFVEFGVNIGGELSNNHWAIVLDKVDSPYKKTLTV
                     IPLTSKNQIDTVLIDEIIAEYPSILLDEYIEKLHKELFAYLKYLDSNNAITEAALSDV
                     YQAYTEQFSNEIIQPKIVDDDNLKRTQSEITDIIELTQYYKKYIKRSYAKCNNLQTIS
                     KDRILKKNRLDPIGKMKVSDNTLDKINEKLKELYLF"
     gene            59430..60875
                     /locus_tag="VVL53_000083"
     CDS             59430..60875
                     /locus_tag="VVL53_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU4182318.1"
                     /translation="MKTMNKVAIYVRVSTTNQVEEGYSIDEQKDKLSSYCDIKDWNVY
                     KVYTDGGFSGSNTDRPALESLIKDAKKRKFDTVLVYKLDRLSRSQKDTLHLIEDVFIK
                     NGIEFLSLQENFDTSTPFGKAMIGLLSVFAQLEREQIKERMQLGKLGRAKSGKSMMWA
                     KTSYGYDYHKETGTVTINPAQALTIKFIFESYLRGRSITKLRDDLNEKYPKHVPWSYR
                     AVRTILDNPVYCGFNQYKGEIYPGNHEPIISKEEYDKTQSELKIRQRTAAENVNPRPF
                     QAKYILSGIAQCGYCGAPLKIMLGVKRKDGSRLKKYECHQRHPRTLRGVTTYNDNKKC
                     DSGFYYKDKLEASVLKEISKLQDDADYLDKIFSGDNAETIDRESYKKQIEELSKKLSR
                     LNDLYIDDRITLEELQSKSAEFISMRGTLETELENDPALRKNKRKADMRKLLNAEKVF
                     SMDYENQKVLVRRLINKVKVTAEDIVINWKI"
     gene            complement(60893..60955)
                     /locus_tag="VVL53_000084"
                     /pseudo
     CDS             complement(60893..60955)
                     /locus_tag="VVL53_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
     gene            complement(60957..61973)
                     /locus_tag="VVL53_000085"
     CDS             complement(60957..61973)
                     /locus_tag="VVL53_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU4182319.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEVGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(61921..62862)
                     /locus_tag="VVL53_000086"
     CDS             complement(61921..62862)
                     /locus_tag="VVL53_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU4182320.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDHKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(62938..63303)
                     /locus_tag="VVL53_000087"
     CDS             complement(62938..63303)
                     /locus_tag="VVL53_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU4182321.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(63454..64512)
                     /locus_tag="VVL53_000088"
     CDS             complement(63454..64512)
                     /locus_tag="VVL53_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4182322.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(64675..65826)
                     /gene="nagA"
                     /locus_tag="VVL53_000089"
     CDS             complement(64675..65826)
                     /gene="nagA"
                     /locus_tag="VVL53_000089"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU4182323.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(65979..67796)
                     /locus_tag="VVL53_000090"
     CDS             complement(65979..67796)
                     /locus_tag="VVL53_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU4182324.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQESKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(67894..69036)
                     /gene="tgt"
                     /locus_tag="VVL53_000091"
     CDS             complement(67894..69036)
                     /gene="tgt"
                     /locus_tag="VVL53_000091"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU4182325.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            69166..70023
                     /locus_tag="VVL53_000092"
     CDS             69166..70023
                     /locus_tag="VVL53_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU4182326.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(70051..70695)
                     /gene="pcp"
                     /locus_tag="VVL53_000093"
     CDS             complement(70051..70695)
                     /gene="pcp"
                     /locus_tag="VVL53_000093"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU4182327.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(70802..71161)
                     /locus_tag="VVL53_000094"
     CDS             complement(70802..71161)
                     /locus_tag="VVL53_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU4182328.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            complement(71172..71597)
                     /locus_tag="VVL53_000095"
     CDS             complement(71172..71597)
                     /locus_tag="VVL53_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU4182329.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(71920..73032)
                     /locus_tag="VVL53_000096"
     CDS             complement(71920..73032)
                     /locus_tag="VVL53_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4182330.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(73198..73818)
                     /locus_tag="VVL53_000097"
     CDS             complement(73198..73818)
                     /locus_tag="VVL53_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4182331.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(73822..75102)
                     /locus_tag="VVL53_000098"
     CDS             complement(73822..75102)
                     /locus_tag="VVL53_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU4182332.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(75163..76647)
                     /locus_tag="VVL53_000099"
     CDS             complement(75163..76647)
                     /locus_tag="VVL53_000099"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU4182333.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(76723..78000)
                     /locus_tag="VVL53_000100"
     CDS             complement(76723..78000)
                     /locus_tag="VVL53_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU4182334.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(78107..78190)
                     /locus_tag="VVL53_000101"
     tRNA            complement(78107..78190)
                     /locus_tag="VVL53_000101"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78154..78156),aa:Leu,seq:caa)
     gene            complement(78200..78271)
                     /locus_tag="VVL53_000102"
     tRNA            complement(78200..78271)
                     /locus_tag="VVL53_000102"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78237..78239),aa:Gln,seq:ttg)
     gene            complement(78282..78354)
                     /locus_tag="VVL53_000103"
     tRNA            complement(78282..78354)
                     /locus_tag="VVL53_000103"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78319..78321),aa:His,seq:gtg)
     gene            complement(78369..78439)
                     /locus_tag="VVL53_000104"
     tRNA            complement(78369..78439)
                     /locus_tag="VVL53_000104"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78405..78407),aa:Trp,seq:cca)
     gene            complement(78444..78524)
                     /locus_tag="VVL53_000105"
     tRNA            complement(78444..78524)
                     /locus_tag="VVL53_000105"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78488..78490),aa:Tyr,seq:gta)
     gene            complement(78537..78609)
                     /locus_tag="VVL53_000106"
     tRNA            complement(78537..78609)
                     /locus_tag="VVL53_000106"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78574..78576),aa:Phe,seq:gaa)
     gene            complement(78613..78686)
                     /locus_tag="VVL53_000107"
     tRNA            complement(78613..78686)
                     /locus_tag="VVL53_000107"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78650..78652),aa:Met,seq:cat)
     gene            complement(78697..78786)
                     /locus_tag="VVL53_000108"
     tRNA            complement(78697..78786)
                     /locus_tag="VVL53_000108"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78748..78750),aa:Ser,seq:tga)
     gene            complement(78869..78942)
                     /locus_tag="VVL53_000109"
     tRNA            complement(78869..78942)
                     /locus_tag="VVL53_000109"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78906..78908),aa:Ile,seq:gat)
     gene            complement(78977..79045)
                     /locus_tag="VVL53_000110"
     tRNA            complement(78977..79045)
                     /locus_tag="VVL53_000110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79013..79015),aa:Gly,seq:tcc)
BASE COUNT        22220 a        17967 c        14037 g        24827 t
ORIGIN      
        1 tcgatcagga cagtcaaatc gaattctaac aatgttttag aagcagatat gtactattct
       61 agtttcaatc tgctatatat tttgtttcta ttctagtttt tcaaaaaggg caaaaaggcc
      121 tcaacatcat aaaaaggcat cctatcaggt attcaaaaac cttgatagga tgccttttat
      181 catagaaaaa ttactggatc tttccctcaa attgagtttt tgtcttgctt ctagactgat
      241 aaacaattat ttacggcgtc ctggtctttc tttgtagcca tagtatgcat cttcgatgat
      301 ttcttgcata tggtctacca ttggaagacg tgggttagct ggtgaacatt ggtcttcata
      361 agccaggaag gataattcac gagaatgttc tttccattct ttttcgtcaa ttccttggtc
      421 tctaaaattc atttggatcc caatacgttc accgagttcg tagacagctt ttgcgtaaga
      481 ttcaacccct tcttctggag tagaagctgg aagtccaagc atgcgtgcga tatcttggta
      541 tttttcatct gcacggtagt agttgtactt aggccatgtt gctgtcttag ctggacgtgt
      601 accgttgtaa cggataacgt atggaagcaa gatagcattt gtacgaccgt ggattgtgtg
      661 gaattgcgca ccaatcttat gggccattga gtgagaaata cctaggaagg cattggcaaa
      721 ggccatacca gcgattgttg aagcgttatg cattttctca cgtgagtgga agtctgcatt
      781 cttaactgag ctttcgagat tttcaaagac caatttaatg gcttgaagtg ctaaaccatc
      841 agtgtagtca ctagccattt gtgatacgta tgcttctgtc gcgtgagtca atacgtccat
      901 accagtatca gcagcaacaa atcctggaac tgtcaatacc aaagcagggt ctacgattgc
      961 cacagttggt gtcaatgagt agtcagcgat tgggtattta cggttgtttg ctttatcaga
     1021 gataacggca aatggtgtta cttcagatcc tgtaccagat gtagttggaa tcgcgatgaa
     1081 tttagtcttc ttaccaagca atgggaactt gaaggcacgt ttacggatat ccatgaattt
     1141 ttggacaagg tcacggaagt ccacttctgg ttgctcgtag aagagccaca ttactttggc
     1201 agcatccatt ggagacccac caccgagtgc gatgatggta tctggtttga aggcacgcat
     1261 aatctcagta ccacggttta cagttgtgat atctggatcc ggttctacat ccgcaaagat
     1321 ttggtaaaca accttattgc gacgaaggtc cagttgttcg atgatacgat caaggaaacc
     1381 aagctctacc atggcatggt cagtaacgat catgacacgt tcaacgtcac gacatttttg
     1441 aaggtattga attgaatcac gttcaaagta tgtttttgaa ggaagtttca tccattgcat
     1501 gttatttctc cgtcttccga cttttttgat attcaagagg ttaatggcac taacgttatc
     1561 cccaactgag ttgcgtccgt aagaaccaca tccaagtgtc aatgatggca agaaggcatt
     1621 gtaaacgtcc ccgataccac caaaagtaga aggtgagtta cagataacac gaatagcttt
     1681 aacagcttta ccaaattctt tagtcaattc ttcgtcagct gtgtggatag ctgctgagtg
     1741 tccaagaccg ttaaattcaa ccatttgacg agccttagta ataccatctt cacggctttc
     1801 agatttcaaa actgcaataa ctggtgacaa tttttcacga gtcaatggct cattttcgcc
     1861 aacttcttta cattctgcag caagaatgtt tgttccttct ggaactgtaa atcctgcttg
     1921 ttctgcaatc caagttgctg gtttaccaac gatgtcagcg ttcaattttg caccagcaca
     1981 gtttttgctg tttgctttga cgccgaagca gaactcttca agaagagctt tttctttttt
     2041 gtttacaaag taagtgtggt aagatttgaa ctctgctaca aattcatcgt aaatttcttt
     2101 atcaatgata actgcttgtt cagatgcaca gaccataccg ttatcaaatg atttagacat
     2161 gacgatatcg tgtgctgctt gacgaatgtt tgctgatttt tcaacataag ctggaacgtt
     2221 tccggcacct accccaagag ctggtttacc acatgaataa gccgccttaa ccatggcatt
     2281 accacctgtt gcaaggattg tcgcaacacc ttcgtggttc ataagggcac ttgttgcttc
     2341 catagatggt tgagtaatcc attgcacaca gttttcagga gcaccagctg cgatagctgc
     2401 atcgcggacg atacgagctg catgagcaga tgattcttgt gctgatggat ggaaggcaaa
     2461 gacgattggg ttacgtgtct tcaatgaaat caatgatttg aagattgctg ttgatgttgg
     2521 gtttgttgtt ggagtaatac cacaaacaac accaactggt tcagcaataa gagtcaatcc
     2581 tgttacatcg tcttcttcga taacgccaac tgtcttagtg tggcgcatat tgtttactac
     2641 gtgttcacaa gcaaacaagt tcttagttgc tttgtcttca aatacaccac gtcctgtttc
     2701 ttcaaaggca tgtaaagcca attctccgtg ggcatccaaa gctgctactg atgctttggc
     2761 aacgatgtag tcaacttgtt cttgatccaa tttacgcatt tcttcaaggg caactagagc
     2821 tttttgaacc aactcatcta cgtgtttttc agcaacgagt ttcttttcct ctggtgtcac
     2881 agttttttta tcagccatat tttcctccat agctttcaag gaacgatatc ctttgttaat
     2941 tttttcacaa gtttattata acgcattatt tcaactttgt aaacagtttc ataaacattt
     3001 cacaaagaat ttttaaactc ttgtgaaact tttctcattt tcttttctta tcaagtttta
     3061 tctttgaagt ttgtgaaatt tatttcaaat attttttatt tcacaaactt gcttggaaga
     3121 gggatttgga aagaaaggga tttacaggta agcgcttacc ttgtgattct aataatacct
     3181 cctttgataa ggaggcttta tttttgttga aaaacgcctt gtttaaaagt tccttcataa
     3241 acgacttctt gttctgttgt tagtttccct tttccttcag cctgaccatt tacaaaatca
     3301 ccttcgtagg tccagccttc tttagattga aaggtacctt ttccgttgaa ggctccattg
     3361 ttgaagccac ctgtatattg gtctccattt tggaaggtaa tggtaccttg gccattcatt
     3421 ttaccacgga caagactgcc atcataaaca atcgttccat tatcgagttt taagacacct
     3481 tttcctggaa tatttagaaa aaagacgagt acagcacaga aaacaatggt aactactgcc
     3541 aaaagctcta aacgtggacg agtcagatag acacgatact tttcgtaaaa attcgtaaga
     3601 ttttccacct taactctcct tttctaaacg ttctaaccaa gcttgacagc cctcttgaac
     3661 acgtcgatag gtttcttcaa aatctcctgt ataccaaggg tctggaacac tttcagatga
     3721 aaatgagtaa atcttatctt gacagtctac tggacacatc tgacgtaagt cagaaatatt
     3781 tgaagcgtcc attccgataa tataatcaaa ggcttcaaaa tcttccttac taatctgaag
     3841 cgatgtcttg ttcttgtcat aaggaatctc atactcttga aaaattccct gagtcccctt
     3901 atgaatcgga ttgccatgtt cccaagagga agttgctcga ctttggattt cgtagttatc
     3961 tgtcattgat ttcataacaa actcggccat agggctacgg caaatatttc ccagacagac
     4021 aaagactaat tttttcatcc catttccttt cttttataga aaacgggagt tcgtgatccc
     4081 gtcttatttt tcaattgcgc cttctagtga agctgtttct ttcagcggta atactgtctt
     4141 gatagcagct agttcaaaag tcaagtaaac tccatctacg tcaagaacta ttgttccctt
     4201 ctccgtatct acttcatcga ctgttccata aagtccaccg attgtaatca cttcatagcc
     4261 tttttgtagt ttatttaagc tttccatacg cttttgagct tgtttctttt gagaacgttg
     4321 cataaagaac atcaaggcca tcatacctac aagcatgatt aaaaaagtaa tatttggatt
     4381 cattgttttc tcctttgtct tttacatagg actactatat caaatttcag ctacttttac
     4441 aagattgtac cttacttttc tggtaagaaa aaccagagct tacgccctga tttttaggat
     4501 tatttcaaat ttcgaacaac ttttacaaga ttttcgacag taaagccata ttctgccaat
     4561 acttttggtg ctggggcaga ggctccgaag gtatcaatac ctagaacggc accatcgaga
     4621 ccaacatatt tgtaccagtt ttgacttgca cccatttcga ctgcaacacg acggcggact
     4681 gcatttggaa gaatctcttc cttgtaagct gcattttgtt tatcaaagac atctgtagat
     4741 ggcatgctga ttacgcggat tttttcgcct tgactagcca attctttagc agctgagaca
     4801 gcaagattaa cctctgaacc tgtcgcaatc aagatggtat caaagtcggc tgcattttca
     4861 tatacaacat aagcaccttt agcaaccttg tcgaagtctg ttccatcttc aacagtcaag
     4921 ttttgacgtg tcaagacaag ggcagttggt gttttctcac ttgtcactgc aaggtaccaa
     4981 gctgcttgcg tttcacgcgc atctgctgga cggaaaacat ttagatttgg catagcacga
     5041 agacctgcta aatgctcaac tggttcgtga gtcggaccat cttccccaac tgcgattgaa
     5101 tcatgggtaa agacataagt cacaggaagt ccttgtaagg ctgacaagcg gacagctgcc
     5161 ttcacatagt cagagaagac gaagaaagtt ccaccgtata cacgaagtcc accatgaagg
     5221 gccatcccgt tcaagattgt tcccattgca aattcacgaa caccaaactg aatgttacga
     5281 ttcaagcgat tggtatcgtc ttgaagtcca tccgtcttga tataagtcat gtttgagtga
     5341 gcaaggtcag ctgatccacc taggaaggtt ggtaatttag ctgccacaac attcaaggca
     5401 tcttgacttg aattacgagt tgcttgagaa aaaccatttt ctaaagctgg gaagtctgct
     5461 ggagtcactt cgactggatc acgtccgtcg atgatggctt ctacttctgc agccagttct
     5521 ggatgagctt ctttataatc tgcaactaat ttagtccaag cttgataagc tgatgcgcca
     5581 cggtctgcaa catgttcttt gaaatcagca tatacttgtt ctggaatttc aaatggttcg
     5641 tagtcccaac cgagggcttg gcgagttgat gcagtttcat ctgctccaag aggggcgccg
     5701 tgtacagcat tagttccttg tttgtttgga gaaccatatc caataaccgt cttcacttca
     5761 attaaagatg gcttgcctga agcttttgct gtttcgatag cagcatggat ggcttccaag
     5821 tctgttccat tttcaaccaa ggcagtatgc caaccgtagg cattgtaacg gtcacgaaca
     5881 ctttctgtaa aggaatcctt tgtctcacca tccaagttga tatcatttga atcataaaga
     5941 acaaccaact tatcaagttt ttgcaaacct gcgtatgaag ctgcctcgct tgagacacct
     6001 tccatcaagt ctccgtctcc acagataacg taagtatagt ggtcaaagat attgtagcct
     6061 tcacggttat atttggctgc caagaaacgt tctgcttggg caaaaccagt agcagttgaa
     6121 atcccttgcc ctagaggacc tgtcgtagca tcaatccctg ccgtatgacc aaattctggg
     6181 tgacctggtg tttttgaacc ccattgacgg aaactcttaa tctcatccat gctgacatct
     6241 tcaaaaccag aaaggtgaag aagagcataa aggagcattg aaccatgacc tgctgaaaga
     6301 ataaagcggt cgcggttaat ccaatttggt tgagctggat tgatatgaag ttgttttgta
     6361 aagaggctgt aagccatcgg agccgctccc ataaccacac ctggatgacc tgagttggct
     6421 ttattaatgg cgtcaatacc tagaaaacga attgcattaa cagatagatt tgacatagaa
     6481 tttcctttct atttgaggtg attattgaat cacacattct attttactat tatatgaaaa
     6541 ttatccgtat catgcaacat acggataacc tccaaagaat atttttatat tatagcaaag
     6601 ctttaaattg aatgttagag tcttgttcaa aacaatcatc aaaaccacgt ggatgatggt
     6661 attctactaa gtgttgatct tgaggataag tgtacttacc gccaacttcc cagataaatg
     6721 gatggaaatc gtattgcaag cgatcttttc gcattttcca aagttctaga atctcattag
     6781 tagaagccat gaagttagac cagatatcat agtgaactgg gataatgact ttggtacgca
     6841 gattttctgc catacgaaga agatcgatag atgtcatttt gtcttggata cctaccggat
     6901 tttcaccata gttattcaaa gcaacatcaa ttttaaagtc tttaccatgt tttgcaaaat
     6961 agtttgagaa gtgagaatct gcaccatgat agatggttcc acctggtgtt tcaaagatat
     7021 agttaacagc cttttgagcc atttcttcat ctgtaacagc caagccagca agttcaccgc
     7081 ctgtctcatc agcaccgttc actgggagag ttaccaagca agtacggtca aatgattcta
     7141 ctgcatgaat cttcatatct tttaattcga tagtgtcacc tggtttaaca acgatgatac
     7201 gttcttttgg aacaccccat ccttcccaga ttcgtccaca gtggtaagga ccgataaact
     7261 taacatgctc taacttagaa ttgttgagaa ttgctgcagc tgtgtatggg tcgatatgat
     7321 cactgtggaa gtgtgaaact aagtaatagt ctagttcgtt gatagcaaat ggatcgataa
     7381 ccattggctg aacacgcaag tttggttgca acttacgaac acctgccata tttgccattt
     7441 ggtgcccacg aaccatatct ttcacttttt tggttgattt tccacggttt gaccaaaggt
     7501 ccatgacaac gttagcacca gctggtgtct taatccaagt accacagttg cctagccacc
     7561 acatggcaaa attgccttca ggtacgactt cttcttcgat ttcttcgttc aaccatgttc
     7621 cccactctgg gaaagtggct aaaatccatg actctcttgt aatttctttg acgtttggca
     7681 tatgttgcct cctttggata taattgttat ctttgagatt agtataccac ccttcaaaac
     7741 actaaaaaag accaagaaaa acacaggttt gtgttcatca ttggactgtc cttcaaatat
     7801 cttccaaaaa agcttgaact actatttctt gatttataag tgtttgaacc tcttcttgga
     7861 acttactagc caacttgctg tctacaatat aagacgaaat aagactagaa agattttcac
     7921 tgaaactttt gctcttatta cgaaatatat tgtgtttaag atagtctagc attttcaaaa
     7981 tatcttctgc ttcaaggata ggattaaccc ttaaaatcgg aaatctgcta tccaaatcat
     8041 cattagtagt aatcactaca tcaacttgtg caatatcttc cacactctta aattgttctg
     8101 ttgtaaatac agtgtcaatt tgctcatttg gaaaataaag tttgcattgt ttcagcaaaa
     8161 gtctcgaaac cgcaacacct tcatcacaaa cgagataaac ttttttcata tttttttgag
     8221 acgatggtgt atactttaaa aatcctccaa tatgaatcgt caaataggca atatcatcgt
     8281 ctgttagccg aataaaccat gctccttcta aaatttccgc agattttcta gtgactaaaa
     8341 ataattctcc atacttggat cgaatttgtt ttgttagagg atttttagaa aaaataccgt
     8401 aagtctttct aaataacaag gctttacagt ggatcatcaa atttcgtaac aaatcatcct
     8461 tgttctcaat ctccattcgg atttgtgatt caaaatacca gataaattct tctaaagcaa
     8521 gttttaattg ccgaaaatca tcactttctg catgaatatc caaatcctta cgataggaga
     8581 ggaggagaac agctacaaga gaaacttcaa gtcctgacaa agaaatttca aacttttgaa
     8641 acaaccgttc tcctaatttc ttagacacct gatactctat tctttttctt atcaaagaaa
     8701 attctttctc tatatcctga tgtctttctt gatactgttc aacattatga cagcttaaca
     8761 gcaaataagg tagaacctgc aacataaaag ttatttcatg atggtttatt ttcttcccta
     8821 aatcttgttc aactaaagga acctgttcct taaaaaattg gttcatttca acagaaagca
     8881 aacactcatc atctaacctc tctttcatct tatcttctaa aatagataca aaagtggcat
     8941 tttcttccat aaaaatatga tatagaagcg attgaagata ctgaatttta ttaagagggt
     9001 gggcatgaag gtggtatccc tgtgacttgc tcacttgcaa gatcacctga tattgttcca
     9061 aagttagttg ataacgaata ctattcaaat cattgagaac agtattccta gatacctctg
     9121 ttaactctat caatttttca atcgtaatac gttctttaga aatacctatc catagtaaca
     9181 ttatcatcat acgttcatgc gcactcatga tataatcgta cgaatctact tccgataata
     9241 gtttacaaca agcatctctc tgctcttccg ttaagtgaat accaattcgt ggaatactaa
     9301 tgatgtgaag cgcctcgtca accagagcag cattgatttt gtcaatgtga taataaattt
     9361 tccttcttga ctctttcaaa tctttcgaaa tcgccataat cgttttggac gtttcttgat
     9421 ccatcaaata ttgaagcaaa tcacaacttg ccttatccag taccatatta aaaaatcccc
     9481 caaatctatt gatatcacag caattctttt tattttacca tatatatcaa gcgctatttc
     9541 taaaaaatct ttcaaaatta cttcaactcc aactttactc tcaataaaaa tcaaatgtaa
     9601 aaagaggagc taaatttatc tttttctcct ccttcatcgt tcttactttt gaccataata
     9661 agcatttggt ccatgtttac gttggtagtg tttttctagt atgtactggg gagcaggtcc
     9721 aactcttgga ttgatttgtt ctgtaaagcg attcatcttt gatacttcct ctagtacgac
     9781 agagtgataa acagcattct ctggattttt gccccaggtg aatggaccgt gattgcgtac
     9841 aacaattcct ggtacttcaa ccgggttaag tccgcgatgt tcaaattctt ctacgataac
     9901 caggccagta tctttttcat aggccacttc tacttcgtcc ttggtcaaac tacgggcgca
     9961 agggattgaa ccgtagaaat aatctgcatg ggttgttccg tagaaaggaa tatcacgacc
    10021 tgcctgagcc caaccaacag cttctgtcga atgggtgtga accacactac caatttctga
    10081 ccaagcctta tataattgca catgagttgg gaggtcggaa gatggtctta aatccccttc
    10141 taggatctta ccatctagat cagtcactac catgttttca ggtgtcaatt cgtcataatc
    10201 cacgcctgat ggtttgataa caatgacacc gagttcgcga ttgacttcag atacattccc
    10261 ccaggtaaat ttgacaagtc catgttttgg caatgattga ttggcatcac aaactcgttt
    10321 acgcatagca ttgattactt gattcatctt acatcaaacc tgctttctta atgagtggat
    10381 agagaaaagc ttgcgcctct tgaatggctg cgcgtgtttc ttctactgtt tcacaatttt
    10441 cagaccacat ttcgattagg aaaggtccat tataattggt ttcctttaaa atatcgaaag
    10501 cttcttccca tttgacacaa ccttgcccga aaggtacatc tcggaactgg ccctttgaac
    10561 tttctgtcac tgcataagta tccttgagat ggagagctgc gatggcatga tgaccaagat
    10621 aaaactcact atagatatca ttatgccatg cagacacatt accaatatct ggatatacaa
    10681 agaggaaggg agagtcaatc tctttttcta tagccaaata tttttcgatg ctactgatga
    10741 aaggatcatc cataatttca atagcaagta ccacctgagc ttcttcagcc cagtcacagg
    10801 cttttctcaa atttttgata aaacgttggc gtgtctgggg tgacttttcc tcatagtaaa
    10861 catcgtaacc agctaattga atcgtacgaa ctcccaagtc ttgagctaat tcgatacatt
    10921 ttttcatgag ttctagagat ttttcctcta gaactggatc ttttgaaccc aatgggtagc
    10981 gacgatggcc tgaaaaacag atagaaggaa tacgaacacc agtttcatag attgctttga
    11041 caacttccaa gcgttcttcc ttactccagt caagtcttgc taaacgctcg tcacgttcgt
    11101 caatagacat ctcgacaaaa tcaaagccta actccttggc aaaatttaaa cgttctagcc
    11161 aagtacagtg tgttggggtt gccttttcat aaattccaat tggacgtacc atgatttacc
    11221 cccaaattcg tttgatttca tccttgaagg cacgcgctgc tcctgctgga tccacagcct
    11281 ctgtaattcc acgacctgcg ataaaggtaa agacatcaat accttcaaag agtttgagag
    11341 tatctacatc tagaccacct gttacagata cacggaagcc catgtcaatg agttttttaa
    11401 ccttattaag gtctttttca ccccaagttt caccagcaag aagagcatca cgagattggt
    11461 gataaatagc ttgtgagata cctgcatcta gccaaagctg agcttgttca aaagtccaat
    11521 cgccataaag ctcgatctgg atttcgcctc gttctcctcg ttcagtcttg atagccttta
    11581 gagctgcttc catagtaggg atggttgcac aacagataca agtcatccag tctgctccac
    11641 gaaccgcatt atttttagca actgttccac cagcatcagc acattttgtg tctgccacaa
    11701 taatcttatc tgggaaaagg ctacgcaaga cttcagccag ttcacttcca acttgaagca
    11761 agcaaacagt tccagcttcg ataatatcta cttcctgacc aacagaaaca gctgctttaa
    11821 tcgctccttg caagtctgaa tggtctaatg caacttgtaa attaggtatt ctttttgtca
    11881 tttctttatt cctctttcta actttccaaa tccaatcctt cgagataagg gctatcctta
    11941 gattcttcaa tcatagccaa gacatcttct ttagtctggc aagcctgtaa acgtgcaata
    12001 gaatcttcta attcaaacag ggcaataatt tgtggaatgg ctacacttgt gtgaattttt
    12061 gaacttgttg ctgctagtgc caacaaaaca gatacctctt tcccatctga aaatacaaca
    12121 ggattttgta aggtaatcaa tgaaaaggca tcactttgca caccagcttc aggtctagcg
    12181 tggggcatag ccatacctgg catcaagata tagtaaggcc catactcttc agtcgattca
    12241 atgatagcat cgtaatactc tggcaaaatt gccccacttt caattaaggg atctactgct
    12301 accttgactg cttctttcca attgttagcc tctaaaccta gtcggatcga gtcattgtca
    12361 attaaagctt gttttaaatt catatctgcc tcctttattt aaagagggag aaggacagaa
    12421 acgataactc tcagtttctg tccagcccct ccaacctttt actgtagtgc ttgactgagt
    12481 ttttcggtga tttctttatc atccatcaag ttatcaagcc caattaactt cccattagtt
    12541 cgcccttcca attcttgaat caaatgaaga gaagcgatta cgatgtcata tcctactgct
    12601 aaacctttag cttcaccgac actgcatgaa ttgactgtaa aatctgtttg attaagctta
    12661 cggagagcat tttcaacctt catcttgata accattgatg aacccattcc atttccgcac
    12721 gctgctaata ctttaaccat tttatttctc cttttctaga tactaagctt cttcttgaac
    12781 ttcaccgttg taatatttct ctttatcttt tgctttggca aattgaagtt gaggaataac
    12841 aagcaagaag agacacacaa gtacataacc aacaatacca aggtatttga agatatatcc
    12901 aaatccaagc catgggaatt caaagtcgat atttccatgg tagccaccat aagatgccaa
    12961 atcgagaagg gccacacaaa gagctcctag agcaacttga aggacacctg atataaagga
    13021 aaggataaca gccgctttcc atccgccgcg tttatcagcg tagaccgcaa tggctgcatt
    13081 gtcaaagaac actggtacaa atcctgtaat aataagaatc ggatttttaa agacgatgag
    13141 caaaacaatt gtaatcaatt gaccaatcaa accaaaggta aatcctgaca agacagcatt
    13201 tggagaacca aatccataag aagctgcaac gtcaaccgct gggaatgaac ctggcaacaa
    13261 tttgtttgaa ataccttgga aggcgtttgt caactcagat acgaacattc ggacaccttg
    13321 catcaaaacg aacaagtaaa ctgagaaggt aaaggctgtt tggataatgt acataaagaa
    13381 atcttgttta gcaggattga atagagttcc tgaagtgatg acttctttat tagacataat
    13441 gtctggaccc aagattaaaa gaatggctcc gaagaatacg agcatcaagg tagcagatgc
    13501 aacaactgta tcgtggaaga ttgagaggaa cttaggtaat ttaagattgt ctaaactttc
    13561 ttctttctta ccaaagcgtc ctgctacttt atctacaaac cagattgcaa attgctgttg
    13621 gtgaccaatc gcaaatccgc caccaccagt caagcgttga gttgcctcaa cagtcatatt
    13681 tgaactaact gcccagtaaa gtccacagat gataccaatc gctgctgtac cgtaagcatt
    13741 gcgcaattgt ggtactaaga atagaaccat aagagatact gttgcagctt gttgtaccat
    13801 gatgtgacca gtaataaaga gggttcttac cttcgtaatc tttcgaagag ctacgagcaa
    13861 gatatttatt ccaaaaccaa tcaatagagc tgtagttgca gttccaacaa aatctggaaa
    13921 ctctgctaca attttgttgt ttgctgcagc aagtccaaag taagggtcga taaccgctgc
    13981 accaatttgg aatttgtagt taagagctgc taagattgga cgaaaggttg taaccaaacc
    14041 accagcaccc acgttaagca acatataccc tactgttgct ttaacaaacc ctgaaaaaac
    14101 gtcatgggca ggttttttca aaagtgcata tcctatcaac accaataaac ctacgaaaaa
    14161 tgcgggattc tgcaaaatat tgctagaaaa ccaatttaga acacttgaaa cgacttccat
    14221 aattgttctc cttttgaatt gtttttgtaa gtttattata taatgcgaaa gcgcaaacaa
    14281 aaagaccaag aattacacag cggactgttc actcttggac tgtatgattt atgatgtttt
    14341 acaaattatc ttattttctc gtagctagat aatagggaag aattttagaa taagacttca
    14401 ataagccttc tacgatttct tcttcagacg aattttctgt cataggaaca tctaccttca
    14461 ctaaaacttt tcgaacttct tgacttctca ccttctcgcg taaagtacga cgcttttctt
    14521 cattcgcctc ccaccgttga ctttgaccat tagagtaggt tagataataa atccctttaa
    14581 cggttggaat gtctaaaact ttggcctgct tgcccagtgt ttgctcatcc ttcttacgtt
    14641 cgatgaaact gacttccaaa gaaattccaa agtcagtaga tgtcccatac aaacgtaagg
    14701 ccatcatagg ttctgtcact tgtccgtctc tctgtagata aacccaaaaa tgtggtctca
    14761 aacgctgggc ctgattcatc cactggctag tctgttggag ttgccattct ggatggcttg
    14821 cttgaaaggc tttggccagt tctgtaaaaa cctttcgggc ctcttgtcct ttgtggcgga
    14881 atgccagcat cttctctcgc tctgctcccg ctttatcagg attacggtac tgcaaaccag
    14941 caaagtctaa atagtctctt atcttattca acatcagttt aacctcctct aactagcaaa
    15001 aaatcaggat aaacctgatt ttacttattc tgtatctaca acaacatagc gatttggctc
    15061 atcaccttca gagtaactag tcacgccatc catacgtgaa ataatacgat ggataatctt
    15121 gcgttcgcta tttgacattg gatctgtttt atgactgcgc ccttcttcca aaacacgagt
    15181 cgccaatttt tgcgcatagg tctgcaagac ttctgcacgg tgttcgacat aatcattgac
    15241 attgattgta acgtagaagg ttctggaata gcggttgtaa agataatttt gagccaacag
    15301 ttgcaaggcc ttcaagactt taccatggta gccgataata cgacctggtt cgttggtgtc
    15361 aatttgtaga ttgatgctac gacggttata atcatttgaa agtgtagcct caacatccat
    15421 gtcatcaata atcgtttgaa cataagccat tacttccgta gctacttgtt caatatcaaa
    15481 gttcgtttca actttcaagc ccaagtcttc tagttcttga ctttcagctt ggtcttgctc
    15541 agtttcaagg tcatcagcgc ctgcttcttc cctcatcgct tcctcgattt gaagttcatt
    15601 taaaatctca atgtgaccag tttcttctaa gatagtgctg gcatgtcttt catgttttaa
    15661 gatttcagcc ttgacttcat cagaaatacc ttgaccttct tcctctattt ttttaatagc
    15721 atcaaccaca tgaccaaggt caacggtttc ttcactaacc gtcttcacag gctcgttcaa
    15781 atcattgatt tttttcggaa cgccttttac tacctgttga tttgctttga caaccgtcgt
    15841 ttcactaatc gcttcaatat ccacttgggc tggtttttta ccaaatagac caagaaagcc
    15901 ttttttctcc ctagaaatga ctttgatatg agccttcatt cttggaatat ctaattcttt
    15961 caatcctttc tggattgctt cttcaacagt tgaacctgta aatactacca ctaccagatt
    16021 cctccttatt tatttcgttt tctgagcctt tttcttggct tttctttttc tattttccaa
    16081 atctttttgt gcctgtacta cggcctcgcg ctctgcgata atcttgaatg gattattcaa
    16141 gaaataggtt tgcaagactt gataagcatt agacactgtc cagtataggg cgactccacc
    16201 tggcgcataa actgcaaaga taaaaatcaa gactggaatc ccatacatca tcgcagtcgt
    16261 agcgccattt cgctcagaca aagctttgtt ggacaaccaa gtacttaaaa aggtgaatac
    16321 tgctgctaaa atcggaagaa caagggttgt atccacacta ccaaggttaa tccataagaa
    16381 atgacctgtc tttaaaaagt caactcttga tagggcttgg aacagggcca aaataaccgg
    16441 catctgaatc aaaatcggcc aaagagagtc tgactgtctg acacccattt ctttaaatac
    16501 tttacgcatt tcctgctcta gtttggttct gctttccata tctcgacctg gatattgttc
    16561 tcgaagcgcc ttaatgcgtg gctgagcttc ctgcattttc ctagaagcca ccatttgcac
    16621 ctgaaagact ggcaagagga ctgtacgaat caagaccgta aagagaataa tccccactcc
    16681 gatactaata tcaaacgata aaaagcgaat gatttccgca aagaagtaaa ccaatttact
    16741 ccaaaaatca gccgattcgg ctgtaatatc gctagttacc ccattagtcg cacaggctgt
    16801 catgattaac agagacagtc ctagcaaact agtcaacttt agtttctttt tcactcccat
    16861 ttccttcccg gtaaatcttt gataatttta atacatggag tagatttttc tccatctctg
    16921 cgtatcccaa ggtttcgact ccttttcgag caatgacaac aaagtcgaca tcttctacca
    16981 gactcccttt tgcattctgg ataatatgcc gaatccgtcg cttaatttga tttctagtga
    17041 cggcattccc cagttttttg ctaactgata gacctactcg aaaacggttt ttctggtttt
    17101 ctaattggta gaccacaaat ttgcgattag caaaacttgt cccctccttg aaaatcgcct
    17161 taaaatcttt ctctcttttt acacgaaagt ttttcttcaa aactcaactc catctattaa
    17221 attactacta ttataccata tttttcaaaa aagccaatca tagcaaggtt ttggacaatt
    17281 tcatcagaaa aagaagctgg aaaaataact ttcccaactc cttgtattga atattttttt
    17341 agtttcactt attttttcaa gcgttcaacg tcacgggcaa tgactaattc ttcatctgtt
    17401 ggaataacca agacacggat tttagctgcc tctgttgaga tgtctcctgt aacgccaaag
    17461 acattctttt catcatcaac atcacaacca aaccacgaaa tccctgagat tacatcacga
    17521 cggaaactct ctgcattttc accgacacct gctgtgaaaa caatggcatc tgctccattt
    17581 agcactgcaa ggtactgacc gatatgtttt tggatacggt caacatacat ttcataagcc
    17641 aagctagcct cgtgattccc ttctgctaca gctgcttcta tatcgcgcat atcgctagaa
    17701 ttagcagaaa ctcccaaaag acctgattca cggttaagaa cacgactgat atcttctggt
    17761 gtgttaaaat cctctgtata ttgcattaaa taaggaatga tagctggatc aatatcccct
    17821 gtacgcgttc ccatcataat accaccaaga ggagtgaacc ccatagaagt gtctacagat
    17881 ttgccggctt tcacagctgt aattgagcct ccgttaccaa tatgacaggt aattaacttc
    17941 aagtcttcta atggacgtcc caagagtttt gcagcttctc ctgctacaaa ctggtgactt
    18001 gtaccatgag caccgtattt acgaaccttg ttttctgtgt aatattttgt tggtagaggg
    18061 tagcgataag ctttctctgg catacttgtg tggaaggaag tatcaaaaac aactacactg
    18121 gtaatgtctg gcaacaattc cttgaaggca cgaacacctg ctgcattggc cgggttgtgt
    18181 agaggagcca acaaactcaa ctcttcaact ttttctaaaa catctccctc aacaactgtt
    18241 gattctttga aatattctcc accagcaaca acacgatgtc caacacctgt aatctcgtca
    18301 taagccttga taatatcgaa acgaatcaag tcatccaata aaattttaac ggcttgtata
    18361 tgattttcaa tatccaaaat ttgttgttca gaacggccgt caaattttac agttgaaatt
    18421 gaatctttca aaccgatacg ttcaatcaaa cctttcgcca atactttttc ttctggcatt
    18481 aagtataatt gccatttcaa acttgaactt cctgcattga ttgcaattgt ttttgtcata
    18541 acatgatacc cctttttaag cgttttcaac tattataaca aaatctattt tatatttcag
    18601 taccttgagt ccatttttga aaattttctt taaatttcat taaaacactt gcatcttgca
    18661 agctagcaag tggataaaca aaaggctcta ctgctatttc atttttcttc tgtaagataa
    18721 aaatagtctt agattgtttg gcattagcaa agagattttc aggcagacta atcatagcaa
    18781 ccagactcgc ctcttctttc agccattctt ttaacaaatc actttgagga ctggtcaaca
    18841 aatcactcgg agctagaaaa atagcgtatc cgtctgactt gaggtactta agcccttgtt
    18901 ccatgagcaa gtgatgggcg taagtatgtt cttggctaga agcaacttga tggcgcgacg
    18961 caacggcatc atcaggataa tagccgacag gcaagtcact gatgaccaca tcgctttctt
    19021 tgagcatttg tgggcgaacg gcatctcctt ggacaaagcc agcctgcaaa ccaattacat
    19081 ctgccatgct agctgccaga tcaatcagca aatcatccac ttccattccc aagtaatcca
    19141 cctttttagt aagcgaggtc aagaaaatag cgcctagaat tcccatccca gaacccattt
    19201 cgaggatagt aatttcctcc tctttaaaca actcttccac aataaacacc aaaagcaaag
    19261 caatagcatc cggtgtaaac tggtgattgg cctgcaaggg ttctgtttgc ccagccttca
    19321 tcaagagaaa ctggtaggtc ttgagccatt cttctttgcg tagtgctaaa cgcttaaggg
    19381 tttgattgtt ctccttgacc tggtttagct cagtttcacc atccagatag atgctatttt
    19441 gctccaccaa ggcgtcataa aagttggtcg ccaaatcact ttggatgact tggacattct
    19501 ctagtaaata ggtataagct tgttcaattt tttcaaaatc catattccta tcttaccaaa
    19561 tttcccttct tttttccatc cttatagaaa aatcactccc tgactaggcg agtgattttt
    19621 gggctactat taaaaagaat tctgagtgat ttccttgaaa caggtcttca gcactataga
    19681 gaatctgact atgcacagat gaaaaaccat gctcaattag cttgttttcc agttcagcta
    19741 aatcaaatcc atgatgatta gcttctgtct tggtaaaatc agcaatgatg agtttcccat
    19801 cttccttcaa atgttgatga aacagtgaga gagccgcatc caaatcaggc atatgatgaa
    19861 gaacccgact aacagcaagg caatcaaact ctttctccaa gggatttttc ggtaaatctt
    19921 gctccaaaaa ctggatattc ttgattgctt gctgctccac tttcaaacga gcttgctcca
    19981 acattttctc agaaatgtct acaagagtga ctgacttagc ctgcttggct aggggttagg
    20041 ggcaaggcta atagacccgt gccaccaccg aaatctaaaa tttctttgtc tgatagaaga
    20101 tcaatctgtt tctcggctgc ttgacatacc aagtttgcga ggaagatatt tttaggggaa
    20161 tcgaaagttt ctgctttgtg gttaaaatca tgtttcatat ctttaattta gcacaaagta
    20221 tagtaaactg aaataagata taaacaaatg aattggagct taacatccat ttccagcaat
    20281 tttctagaaa ctacagtgga ctattctgga ttcaacgcat tatagttgaa ttgactatga
    20341 attctctttc ttttcaggct tctcttctga ttttttcttc tccacttttt cgcttgaatc
    20401 ggtcgctacc tcttcctttt tatctctttt ctcttctttg attttgactg aaggaaacag
    20461 aaactcatat tggctcttat ccgtacgaac cctcgtcact aagccagttt tcttgttttg
    20521 atagcttacc tgacctagat taaaaaactg ttccccactt tcttgctcaa ccttatcttt
    20581 ggttcgttta gccatagcaa aagcaaccaa tttttcttta ttcaaagcat agtcttgata
    20641 gtgggcgact tgtcggttca aataaaattg caacaaaaga ctaaagatgg ctgctatggt
    20701 gactgcgtag aggagaacac ctgccttaac ttttttcttt ttccacacga tagatgaact
    20761 ccctttctaa gcctttttgg aactggaaat gaaagcgaac cagttgattg tcctctgtaa
    20821 tccgtacaga tttgagtcca taaaccatag gctgataacc tcgaccacga gcattcgttt
    20881 tacggaaatc atctgacttt gacttaccga tggcgatgtc cttgccatct tgcttcatgt
    20941 ataggcgatt gccttctact ttttcgaact gcgaacggtc taattctacc tcaagttggt
    21001 ccacaaacaa gagccactcc ttttgctcgc tttgttgctg gtagcgaact tctgaaatga
    21061 ggagctgact catagcttga aaaaggagta atcccccact gatgacaatg agggcaagca
    21121 gggattctaa caaggtaaaa gccttgacct tatggctctt tgattgccaa caactgttct
    21181 gaaccatggt agacctccaa tcctttttca ctagaaaata cctgaatctc aactccgttg
    21241 atgcttacct gattttgccc cgtctgcagg gccatcttag ctaccctcaa gacttcttcc
    21301 ttttgcaaga tttttgcttc ctcttgccta tttttttgaa tttgtcccaa aaggagggtc
    21361 gcaatgctgg caaagatagc tagagcgact actgcttcca gtaaaatcac tgccctaatt
    21421 ttttgtttcc ttaatgcgtt taatttttcc atttcctaga tataattgat agcgaatcgc
    21481 tcctttactg gtctgaaatt caaccttagc cagggacgaa ttgcccccag ctcggtcaaa
    21541 tgtaatactt tggcctgatg gggcctgaat tcctttaggg actggcaact tttgactgcc
    21601 attgctaagc gtctgcccat ctaagttcag actagtcttt tgctgactgg ctacactgcg
    21661 tttttgggtt tcccgataga gttcttcaaa ctccataaag aaaatctgtt cctctaccgc
    21721 tgaaaaagtg gactggacag agccggacaa gcccaaggca aggatactca caagtcccaa
    21781 aaccaagaga ctttccagca tggtaaaggc cttaatcatt gactttacga tttgctcctc
    21841 catttttatc attgtattct ttataagctt tagcctgttc ttccgtgatg cgtccatctg
    21901 cttgtaactt tcttaggcta gcatcttcat tcttttctaa gctataaagt tctgcctggc
    21961 tttccaccac cttaacaaca gctgcttttc ctttgtcatt gactgcttct ttttgcttgg
    22021 tcagattagg tacaaagagc aagaaaagca cgctgataat cagcaagacc accaacatct
    22081 caatcaagga tagtaaaact ctcactcttt tctatctcat acatccttac cgaatgcaac
    22141 acgagatcac gatacttcca agtgctgacc agatactcaa taacctcttg gtcctctatc
    22201 ctgcattcca taagtaataa tagcttgacc gtgtattcat tcttcaaaat cggaacctga
    22261 taagtcacat ctacccaatg ctcaaaacct aaatctgtct gctctacatt tgctagttca
    22321 atatttaaaa tcttcatttt tattcctcct acttatctat tcgtaaaaga agataaaaag
    22381 ttatgaaaaa atcattattt ttttattttt gacaaaacac cgtttttgac aataatcaaa
    22441 aaataaaaag gagctattat taacaaaatg gcatttttga caataatgct cttccattcc
    22501 tcctccctat tcttcatgta aacgattttt tgaagaatca gagcaaacaa aaaaaccgca
    22561 agcctgagcc tgcggtgaaa gaacaattta gaaagtttcc tttctattta ttttactgta
    22621 atcaagccat ctggctctac tgtgaattct ggcttgtctg ccagtgttcc gtctggtttg
    22681 aggtagtacc agcctgttcc gtccgctgac tggataaaag catttgatac catggcgcct
    22741 tctttagcgt ctaagtagta ccaagtgtcc ttgtacttga cccagcctgt cttcatggca
    22801 ccttctacgt cgaaatagta ccacttgtca gcgattttct tccagcctgt ggccatttcg
    22861 cctgattggt caaagtagta ccaattaccg tctgtgtgct tcttccagcg gtctgcaagc
    22921 atatagcctg agccatcgaa ataataccag gtaccgttga ttttctcaaa cttatctttt
    22981 ggataagagc cgtctgagtg tacgtaccag tagccagtgc catttttctg ccagcctgtc
    23041 gcagcgctca agccgttttc gatgtcttgc ttaaactgtt cacggctaat gccccaactt
    23101 gcaagatatg gatatggatc cacatggtct gagtggttgt ttggttggtt attggtacag
    23161 tattcatgcg tcttgatacc tgccaagtcg tctgtatcaa gagtcttcgg caagcctgct
    23221 tcgtccgcta gattgcgtag caattcgata tagaggcggt agtcagccat aaactcttcc
    23281 ttagttgaat ggctttcaat cagttcaacc gctgcgtaac tctcagtatt ccaaccgccc
    23341 ccaacatccc aacttccgtt gttcacagga cctacttgca tgacacggcc gtttccgaca
    23401 acatgtgaaa agaaccctag ttcagggtcc tttctatagt gataatccgc ttcattctgt
    23461 acggttgagt tgcggttacc tgttgagtga gcatgtactt gtcgataagg ctgcacccca
    23521 acctggggca agcctgtacg tagtctgttt ctatcgatat ccattcccta tcgtcctttc
    23581 catgcgtcat tcatctgctt cactgctgac tctacgaagg tgtctaagtc tttgtcagtc
    23641 atgctaatat tgtattttgt aagctcagca cggactttag cgcgagcctg tgccagtttt
    23701 tcatctccct tgtagccagt ttcagcagct acctgctcca cggcatttac tgcatttttg
    23761 gccaagattt caacgatttt gatggtcttt tctccacctt tttgaaccag gtagtccttg
    23821 actgccttga ctgcgatacc agccaaaata acaaggatgc tgattgctcc attagtaatg
    23881 atttcagtaa tttgttgcat ttgttattct cctttttcga tttcttccat gcggtcgttc
    23941 atgcgaacca tttctttttg aatgtctccg accgtgtgag tgattgtggt taattctgta
    24001 gtggtctttt ctaggtgagt catcaaacgc tcttctcgtc tattagagtc ggcctttgat
    24061 tgctcgtgca aatccataat cttcttctct cgcttgtccg aagtcttgat aagatatcga
    24121 atgataataa aaaaaagcaa gataaacaaa atcgcccaag ctacctgact ttgagcgatt
    24181 ttttcaactt cttcaattgg catacaacct cctattcttt aggttctacc gttggaaccg
    24241 tccagtcagg attgccctct gcatcaaatt tcatgatata gaattcatga ttcaacagaa
    24301 cggctacgtt gattgttgcg attgtaccac cccactggtt gaacgcccaa acggtttcaa
    24361 catccttgaa ttggcgacgg ccatttacga tcacaggacg tttttgaaca tcacgataca
    24421 tatagaagtc atcgcttaca ttcttgcaac gaatgaactc tccattttct ttcatgtagc
    24481 gcaaagcact cgcaagatca aatggttctg tgatttttgt aaggtctagt aagttatctg
    24541 tgttttgaat tgtttctgcc atgtctattc tcctttgtct gctggtttag tttgttcatc
    24601 aagcagagct tccagctcat ccactcgtgc ttgaagtctt tgattctctt ccctttgctc
    24661 attcaactga atactcaaga tattacttgt aatcatcgaa tctgttgaag ttgttgacat
    24721 ttcacgaatt gtcatttgta aggcttggtt aagctgttct gtgttcattt tctaagttct
    24781 ccaatctgtg tgttcgtttt ctattttcaa gagcaagctc ctgaattgct ttaaagtgtg
    24841 atattggtca acatgttgtt attcatgcta ttgttttctc cattttttct attttttgat
    24901 ttaattcttg aatagccttg attaagtaag gaactaaagc ggtatagtcg atatgcagat
    24961 agccatctgg attctcagga tctcgtgaga caattcttgg aacgatggtt tcagcctctt
    25021 gagctattag accaatctcc tcatgtttct tattttcgat gaaatcaaat gcaaccattc
    25081 ttaatctgtt gattttatcc aaggctttca cagctgtatc tgtgatgttc tcttttaagc
    25141 gtctgtctga tttttgttcc atccaatact tcaagctacc gctaccgacc tgattccacc
    25201 aaacaaccgc attccttccg cctttgggat tccaaccatc accaagcaca tctttacttc
    25261 caagttcgat accatttgaa aacacaggag aacgagaaaa agtagtattc ccatagaagt
    25321 ttgctctcga tgaattcgaa aaatccactt gatcataaaa accgacttca ttcctacagt
    25381 acattttccc atcagtattg acgttccatg ctttaggtcc ggcaaagttc caattatttc
    25441 cccagttcgc ccagaaggct gtccggactc catacccggc accattcccc ataccaacag
    25501 agaactgatt gacacctgaa atccagcgac cgccaccctg gtcaaattga ccaagtgtga
    25561 atccaccgat tcggccttga taggcttcta ggaaggttga gctagaaatg acggactcaa
    25621 ccttagtaga gaagatacgt ttagatatca gttcgtaaat aaaagcatca gttgcagtta
    25681 attttctaat aagcgcattg tcaactttca acttctcagc agttaccgct tcagcgtcta
    25741 atatcgtagt cgtgaccgaa ccagcttcaa aattgcccgt tttcagctta tcaaccatag
    25801 cagacttgat gactgctctg tcaatcaggg tctctccagt gatgtgggtc aatttcccaa
    25861 caaagcggtt atgtccattg gcgccaagat tgattccaga gatgatatct ccagccgagt
    25921 tgatgttttg aactgcccat gagccagtta gttggctttg aacagagcga atcgcttcgt
    25981 ccgtgtcttc aggagcttcg gtgtacggtg ttgcatacga tcctttttca agtttaagac
    26041 ctgcaatata tagatttgta ttttgattaa gtcgttctac acgaggaaag atgaagccgt
    26101 ccacagtaat tttgaaggta aatgagtaac gtttccattc acttgttata gcgaccgatg
    26161 tttcagatgg aacaccccaa cctttctcaa cagatccatt tctatttaca tagaaattta
    26221 ctgtatcatt cttccagtcg ctcttcatcc ataagctgaa tgtatagact tcaccaattt
    26281 ttgcatcaat ctcttgtcca acgccattcc acgaattatt tttaaatagg acatcaacac
    26341 ctttgtattt ttcagcgtgc tttttccaat ttgcaccctt gtttttccaa ccccctgaga
    26401 agtcttttgt gcctttcaac aaattttgac cattgctggc atttttagcc acctcaacct
    26461 gaaacagttg attagtcaga gccatgcgag caaccttatc cgcaattcca ttttcagtat
    26521 tgcccaaaat tcgctcgtaa agtttactgg tttccttaac acgctggaag tcagtagtct
    26581 ctacttttcg cgctagttga ttggtcacat tcgcaaattg actatcagca ttcgctttgt
    26641 ttgcagaaac ctgatcagat attctaccca tttgtcgttc agcattatcc ttgtttgtag
    26701 cgacctgagt ctttaaattt gaaatctgat tatctgtgcc ttgcttatta ctgtttatcc
    26761 gatttgaaat atttgaaatc tgagtagtgg ttccttgctc actgcttgta agtctatttg
    26821 atagaccact gatttgaccg cccacatctt gcttataagt agttatctga cttgaaatat
    26881 ccgtgaactt accatctaca gattgacgat agctagcgat ttgactagcg atgtctttat
    26941 tcgcactagt tttaacagct tcaatccttt gattgatacc cttaacatct tcttgataag
    27001 tagccttacc aacgaaatca cgattgacct gctcacggac tgctgtcgct tgtctcgcgc
    27061 tttcctcacg agtatagcgc tgtagggctt cctgtcgctg accgtcttga ccaacatagc
    27121 tctcaactgc tgccatctta gtagataggc catcagctgt cctcttgaac tcggttttag
    27181 cgactaaaag ctcattttct ccgtcttctg gtgctggtga ccagtccgtc gccacactac
    27241 cgatttcaac cttgattcct gttacccaag ctgtaccgct tgtagcacct tcaagattga
    27301 atcgcaatga tgtcttcaat tgatcaaaat ttgttttttc agagtagtca taagtgaatg
    27361 taatatattt ccaatctgcc gaacctttat acataccaag cgtagcataa tctggaccac
    27421 tctgtactcc ggtctcacta ttttttctaa aaagataatg tttgaagcaa ttaaatacat
    27481 tccaaaaatt tcgaccttgg actacatttt cgtacttgat ccaagcgcta aaagtaactt
    27541 tttgatacaa ccttgagctg aaatctggtt caaggttgaa cgttaaagta gagttgttct
    27601 ctaacctata acattctttt tgacctgtga catggttttc aggtaatttt tcaattacag
    27661 ctccaaccgt cttggatttt atccatagat tccggcctcc caccttcatt tttgaaaatt
    27721 cttcacgcaa tttcccagct tcagatacaa ctaaagtctt atctgcctta tccttggttg
    27781 cgttcaggat ttcctgacgg atagagccgg ctcgcacctc aaattcagcc tgactcaact
    27841 tctgatttag cttgttctgc gtgtctgtct caagactctt cacagattgc cggatatttt
    27901 cagcagtcac gttgagtgcg ctgatatcca ctttggttct aaggccttca gtcagacgat
    27961 tcacaccagc ttcgagcgag ttgactcgtt gtttaaaggt cgattcaact gttgaaatct
    28021 gaccttctat atcttcagga gcttctgaat aagaagtatc tacatcgctt atttcaaact
    28081 tcggcatcca aatccaaacg gttccttcct ggttgaaatt gaacaaccat tcatttgtgg
    28141 tctgcttgga ttcgtttgtc caaccttttg gaatatggac aacatatcgt ttaatttctg
    28201 tcgacaatgt cacatttcca gttttatatc cgatattccc taatcgagat cttagcatta
    28261 ttccattttt atttgcctta gcataaaaac taatggttac atcttgatta gtcgtacttc
    28321 cgggaattac tttcccgaat tgacccagaa ctggataagt aaccttggga ttccctccat
    28381 cacggccaga tggattcaga cctataattt taagagcgtt atatccaaga tacttacttt
    28441 cgctatcgat agtagccgta tatgtactcg ttgtccaaat tcctgttttt gaaatatcct
    28501 gcttgaatag tgagttcaag aatagatttc gaccggatgc ctgcacactc gcaatccgac
    28561 tagctagctc ctcagctgtc tgcgtgagtt ctgacttgct ggctttacca ttggccaagt
    28621 tggtcagttc tgacagtcta cgagtcgtcg tctcctcata cgtcgcttgc gctgacttca
    28681 cgccagccag ttcattttta gtccggctaa gtgcttcaac ttgcttggca atctcagctt
    28741 cagcctgtgc ttgcttcggt cgaatatcgt ttgcgatagt tcgttttaga acatccaagt
    28801 cacctgacag agccgtttgt gcgctcgtag tctgcgactt aaacgcttca agtctagcaa
    28861 cagaatccag ctcaatccgc ttagcttcct gtgcaagcag ggtacttgcg ccatccagtt
    28921 catttttagt ccggctaagt gcttcaactt gcttggcaat ctcagcttca gcctgtgctt
    28981 gcttcggtcg aatatcattc gcgatagtcc gtttcagagc gtccaagtca cccgacagag
    29041 ccgtttgtgc gctcgtagtc tgcgacttaa acgcttcaag tctagcaaca gaatccagcc
    29101 caatccgctt ggattcctga gcaagagaac tacttgcgcc agcatttcgc aaagcttcct
    29161 cagccttgcg cttagcttct ttcaatggcc cgttgtcaaa gctattaaag cgctgattga
    29221 tagtgtcaga cagttctctc ttgacttctt cagctctggc tttggccagt tctacttgat
    29281 cgttaaagtc ttttttgatt ttgtcgacct tttggtcaaa atctttatct gctgcttcaa
    29341 tctgcgcttg gattttcgct tcaaagccat cttgttgctt aatctgcttg gtaatcgtac
    29401 cctcgtaaga atactgggta tcgtttccag ccttactatc tgcactgata cgacctctca
    29461 gaccaccttt aaagataaag ctctgactta agacaggaac tttaaaagtc tctttcttgt
    29521 tggtctgaat ggttacccac tgcccaacct caagtaacaa atgtccttgg tagttgagat
    29581 tatacggata gtaagttagg tttttcagtt tgtaatacaa gtcatttaaa gcgctctggg
    29641 ttatgaagac attatcaagt tccaaagacc gacctgttgt cataccgacc gtcagagact
    29701 tcttatccgt cttacaagtg ataccagcta tctgatactc gatttcactc ttggtcaatc
    29761 catgcaagaa gtaactgtca gcgttaatcg tgatgttgga ctcggtcaaa tcacggattt
    29821 ccatctttcc ttctcggttg aagaagcaag acatcccaat catctgagtc atagcgctca
    29881 gcatatccct gaacgaaagt ttcttacctt caggaacttg ctcgatatgg taacgcatag
    29941 cgctgattcc gaaatagtca ttcgctaact caatgcctgt tttcaggcag atttcctgaa
    30001 taacctctcg tacttcagct gggaaatgca aatccgtcac gtactcacga ttgagcttaa
    30061 acataccgtc cataagttca agtgtggttg tgttgcggtt tcggtcaatc tcaatatcgt
    30121 tgatgaagta ttcccccatc ttgacccact ggtaggtatc cccaaccagt agaccaatct
    30181 cagggtgcag ggtatccagc ttattgaacg tggtaatgat actggtaaag gtaattttac
    30241 cgctaccagc gcaggttcca ccaggcttat aagtatcgcc cttgatgtag ccatactcaa
    30301 aactagcctc tttgatatcc cgtgaagcat attcaccaac acgaatagcc agcgtccttt
    30361 ccttggcaaa catggctctg tcaaattgtc gtctagttaa agcgtccatt ttcttacctc
    30421 tctaccagat taaatttagc gccagaccaa ggtttaagct tctcagtaaa ggtatagcta
    30481 ggagctgtcc tatcaccgac atagaaagtc tttgtgactt ggccatccat ggggtctgga
    30541 taagatacct caaaaaattt agatgataca gcatgtaaaa gctgacttat ttctccctga
    30601 gtcatcatac cccattcaca gtctagtttg cgtttggtcg tgatacggtc acgcaccatg
    30661 tcgccattgg cattacgccc tgtctctcca tcgatatctt gaataccgac ttgaaaagat
    30721 ttgggaggct tcacagccac cccattgatt gtcaattgtg ccatttaacc tcctaaatct
    30781 tgagcaaggt ttgacctgct cgttcatgtt ccttgttgat ttcttggata gctacccgtc
    30841 cgaactcatg gcctgcgatt tgaataacga tgtcgccgtc gctagaaaaa ccaccttgtg
    30901 gactaatacc agccatggca tttactaccg cactgctgac tactcgtcca agtgtttgga
    30961 taaatcctgt attttcaagt ggtacgaccg cctctttacc agcttcacca atcatggcga
    31021 ttgttggact atcgacgata ccaccacggg caagacgagg gaggctaact gtgcttacac
    31081 taccaaccca tcctagacca ggtaagtttc tgacaacgcc taaaactcca ttaatcattc
    31141 cgatgaagcc attgactaca ttttcaatcg ttccaagaac cgcattgacc gcactcttaa
    31201 acgctccacc tacagcgcta ccaacctttt gtccagcatt gacaaatatg ctttgaaccg
    31261 ttgaccatac accgctgaag aagccaccaa ttgaactaaa cgcgttcttg actgcattat
    31321 aagcactagt aaacatattc ccaaaccaag aagatacatt cgcaagaaca cttgtaacat
    31381 ctgcccatct ctcgccaaac cataaaccta gtttgctaaa gatgtttgtt agaccagtcc
    31441 atgctttttg gaatatgtca gtaaaccatg ccccgatatt agccaaagca ctagtcacat
    31501 ctgcccaacg ttgtccaaac catgagccga ttggagtgaa gatattaacg atagcgtccc
    31561 atgcaccttg gaatacacca gagaaccact ctccgatacc agagaagatg ttgacaatgg
    31621 catcccatgc ttgctggaac ttctcgccaa accattgacc tatcggctca aagatttctt
    31681 gtagttttgt ccatagacca ctgaaaaatt cgccaatagc tcgacaaata ccactaataa
    31741 aatcacatag tccttgccat gcagttttag caaactcaac aacagtgtcc cagttttggt
    31801 agagcaagac accgatagca atcaaagctg cgattgctgc aataatccat gttattggac
    31861 ttgtcaaaac tgctaacgct gcattaaaag cccatgttgc agctgtagcg actcctgctg
    31921 caacagaatg tgcaaattcc gccgcggttg ctaatcccat tttcgctgca tgagcagtcc
    31981 atgctagagc tgatttacca agttctaaag cagtttttcc tagctgtgca attgttttac
    32041 ctgaattgac cacaaaatct tttgcatata aggtgttcaa atagattgtt tcaccaaaac
    32101 tgaccaactt atcaaatgtc aatgctttaa tagcaagacc tagattctta atccctccaa
    32161 caatcaaaga gaccttacta cctaacaagc tgaatgctcc tgcaagtccc ccggcttgtt
    32221 cagcccatga taagaaatta atcgtttgcc aagttgttat caaagccaag ataggttctt
    32281 tgttttcttt acaccagtca gaaaaaacgg tgaaaccatc tgccactaac ttaatagcat
    32341 ccgccaatag tcccaaagtg gctaaaaggc cacctcctaa taaatctgaa attccttcaa
    32401 tactaacacc gaatactcct gataaaaact cagcaaaagg ttgccaagaa ttctcccaga
    32461 ggatttgtat gatatcaatc agtccgttaa aagcattagc aatagagtta atagcagggg
    32521 ctacatgttc atcataaaca cgacttaagc catcgccaaa tttgttaaca gacctttcaa
    32581 tgctctcaaa tacaggcgca acagtatcta ataaactttg gaagactgat gaaattttag
    32641 gagcgcttgt cacaacgact ttttcaaaac ctttaaacaa acttcctgct aatttactac
    32701 caacttcaac aatggtagat gtcaaactca acagagttga cacaatagcg ctaccgatac
    32761 gaaccgcacc agttgaggta atgacgtcgt agaaagtact agaaaagtcc tgagcgatgt
    32821 ttcctactgc ctcggaaatg ttaccaatat tatcaaacaa agcgactagc gccctggtaa
    32881 tgcgttcttt ttgccttcca aggccatttg caatactttc ggcaaggaaa acaccgatac
    32941 ctagcccgat agttgttatt gagcctgtca cttgccctaa agcataagca attttctcag
    33001 ccattcggtt aaaggcattc acaacccttg ggtcagtggc gatttctccc attgtcttag
    33061 ctatttggtc taaggcagtc ttaatgcgtt ctataccttc tggtctaaat gctgcatcaa
    33121 aacctttctt gaagaggtca aacaaccctt tgagcttatc tccaagacca tcaaaaatgc
    33181 tcttgaattt gttgtccatg tcggtcaact cgacttctgg caagatgtct ttgaaaggtc
    33241 cgccaccgcc tccctttcct ttaccacctt tgccaccgcc tccagacccg cctgcgtcgt
    33301 catcttttgg tttttgcaag atgttaatct catcaaatcc caaaagacct agcaactctt
    33361 tagcggcctt cttagcgttt ttggcggagt ctccaagatt gtcagcaagt cctcctgctg
    33421 aatctccagc gtcgtctact gcgtcagcaa ggtctcctgc tccgcctgca gcatccttca
    33481 tggcgttacc catgtctcca actgctccac caacaccatc tttcactgtt gctttcttgt
    33541 tgaacatcaa agcgataaac tcagcgagtt tagcagtaac gttctttaag accatagcaa
    33601 aagagttcaa gacaggcata atggcattga taatcggtaa catagagtta ccaaggttca
    33661 atgctgcgtc cttcatcagc gacttaaata ggctgatacg accatttaca gaattagaca
    33721 aggtatcccc atacttggct gtagcctgtt ccagaatagc cataaggcgg atttgttgct
    33781 gggtttggta atccaactgt tgccagctct gtccgtttgc gaacttctta aaggcttcag
    33841 tggactcaat catagccacg ttgacgttga ttcctaggtc ctcaatcgct tcggtgttcc
    33901 ctagtaaacc tgagcgaatc cgctccataa cgtctgtaat cgtgcgccct gaaccttcag
    33961 caacaactgc cgatgtctgc aacatcttag cggtataggc gcttagcttg ttggtatctt
    34021 tgataaatcc agaaaataag tttgagtaga ctgcaccgta gttggtagcc tcacccacac
    34081 ccatattcat agcgttggca ttatcgttaa cccattttaa gaaagattgc gaactctcgc
    34141 ccatctgtcg cttgatttgg ttcatagacg ctgatacttc aagagccgtc tgcgttgaat
    34201 acatcccaac atcaagtaat ttcttaccaa ggattgcaaa accagcgaac ttagccagct
    34261 tgccaaacgc actaccgatt gagctcgact gttcacgaac tttggcagta gcatttttca
    34321 cttggtcaga tgttcctttg acctgattct cgacttcttt catcttcttc ctgaaaggcg
    34381 ctatctcagc gtcaatcatg actttcaatt catcaagagt tgccatttac ttcctccttc
    34441 ctttttcgat tatgtctctc tgcaaattca cgcatccgtt ccttatgcaa caaaagtgct
    34501 tgtttctgtc gttcctgttc tactgcttgt tgttcttcta caaacaactc aggcgcatat
    34561 tcccagaact caaaggcctt ggcatctttg gataacaata aagaaacgtg gttggatatc
    34621 atctgcgaaa gtctgtatga gtcaataatc ttttctttac gctcttggat tttgacacgg
    34681 ttatagcttt caatcatttc tctgatttca agtaccgtca aatcccaaaa atcaagaggc
    34741 ttgcccccga tgtccaaaaa cataggataa agcctctcaa taatctgagt taccgttaag
    34801 attacttgtc tactgtcatt ttcttcttgg aagttttctt gtccttgctt cctcgtggag
    34861 taaaacccga tacttcaaag agtggcatca acacctctgt catgaaggtt gtttggtctc
    34921 cgccattgtc cacgtattca tcgtataaat catagacatc ctcaagagaa tacccatgtt
    34981 catactgctg caaggctccg tgaactaaca acaacataac tttcaaaggc ggtaaagtga
    35041 actcttcgcc agcttcaggc atgaaaatct tcagcaagtt catgccgatt ttttcttcca
    35101 cagttgcagc ttgatgagat gtcaaacgta gcttcaactc tttttcgtca gtaactttcc
    35161 aagttgtgta ttttaacgcc atttaattaa cctccaatac catcagtaaa tgtaagatta
    35221 gactgcaagg caatcttaag tgtgaaatcg ataacggcat tgacaccgcc accgccaagc
    35281 ttaacagata cttggccttc aaaaatgacc ttagtaccgt ctggataagc ttgttcaaag
    35341 tagagtttct tcttgtcgtc tgctgcctta cgcaatacac gataaggagc agttgcgctt
    35401 gaattattat aagagaactt gtactcaagt tctcctatgt ccccaatacc aaactcgtac
    35461 ttcttcaccg tatcttcaag agtagtattt tctacttttt cgagttcaat accaaactct
    35521 ggtacttctt tcaatccaac aagtttagta tagctatctt ttgtttcgcc ataagaaagc
    35581 gtaattccat ttgctaacat gtttaattct ccattctaaa ttgaaaaaca agctctgagt
    35641 gtaagtcgac gataccttca aaacgcatga ccttatgtct caaatgagac gggtctggca
    35701 cgtcttggca gtcagttctt cgcaaaccta aagactcaaa aatctgattg attttaacag
    35761 ctaactcact agtgctagta tcatcaaaga tatccacctt gtagcggata gaggattttt
    35821 gttcctggtc atcaaaccaa tcacccggct tgttttgttc ttccaaaaaa ataacgactg
    35881 ggaaagtctc ccaatcgcta ggataagtat cggtcacatt atctgcaacc ttttgcaatt
    35941 ctttataaat aacaggcttg atattaatca ttttatttgt tctcttatct ttctacggac
    36001 ataattcgaa atattcttag acacacgctc ttgattgtct ctcaaagctg gataaagata
    36061 aggctgggca ggttgaccat acatcttgta gaactccccg attttttgaa agtggtacgg
    36121 tcctacattg atttggtctt catgtacata ccacggacta gacttgtaag tcacgctgac
    36181 ctctggagaa ataccagaat ggctagcttg tcctattggt cccgttccaa actcaacata
    36241 aggagcgtat ttaaggttgg tgtaaacttc gcctatagcc ttatctccgt ccatttttgc
    36301 cctagttttg atactagtta taagctctcc atctctcgct ggtgcgagtc ttcttgcatc
    36361 tgcttggaca acctttatag tagcattgtg caccgcacgt aagacgatat cctcgccagt
    36421 ttttttacta gccaatcgtc tacatttagc tataagccta tctgccccta gtagccctga
    36481 cacgctctaa ctccaaaact tgatgatgtg tgtagacctt tttagaaata accctatgag
    36541 tcacttccgt ctgactatcg atacacacac catcttttac tttgatagta gctgacttgt
    36601 tggcatttgc gttcaaaata tcattgacac gctcgccata caattcagat tgtagcttgc
    36661 tactagctgg ccacaattca aggcggactg tctcagcttc cttggcatac ccttctttcg
    36721 cgacaccttc ctcagtgaca gtcttttcaa accgtcgcat tggataaggt ttcagtctac
    36781 tctgcttcaa aaacatgacc tgccaccctt gctagcctgt gcatgcgtat tcgctgtaga
    36841 agatccgtag acaggccgtt ttctccgtag actactgcta taccaccctc ggttctagaa
    36901 tgctctcctt ccgctcctga gcggttgtgg agttcgatag caacctcagg tattaaaaga
    36961 cttaaagcag gtgtcaaaga tgtgcgatta gtctctgaca agataagatt tgtagccctt
    37021 gtttggagca acatgagaag ctgagtatct tcttcgcctg ttaatttctt cagcaactct
    37081 atagacatat caatcctctt ctaagaactc aggtttagga aagatttcct caagaacatc
    37141 tgagatagcg acaccgttgc tggcaatatt gtcagccagc tcagcatagc gctcctcagt
    37201 aatctcaagt tcctctcctg ccagtcgttt cacatttgat tcccaatcat agaaatcttg
    37261 tttgatttta aatttcataa ctcggaccta tttcttacca gttttttctt tccagttagt
    37321 tgtatctgtg tctggtgcgg ttgatgaatt agaaatatcc ttaaccgcta catagacttt
    37381 atcggcatga gtaactgtgt caccttcttt gtaggttgtt ccagttttcc acgctttagc
    37441 acggtttaca actttacctt gagtagatgg tttagcagca ggcttagaat ctgcaattgt
    37501 gatgatgtat ttcttgaagt gttcaagaac aaatgcacca gtgtaaagca attgctctac
    37561 caattcgcca aatcgccctg gaatgttatc gttgtactta gtattatcta cttgtactgg
    37621 agatgtaaca acacctggag cagtagcaag ggcattaaca cctttcagga atttagaagg
    37681 aaccttatag actgtgtaat catccaattc accaacatat ccttttccaa ggactttctt
    37741 atctgcgtca ccatgtggta gacgaacgat ttcagacttg atcgctttgt agaaacttgg
    37801 agtgacgaag agcaagcgtt cttttgtaat tccaagctca tcaagtttct cagacacatc
    37861 aagaaccgca ttataagcgt tgttcgctcc tgctgtttta cccatggcaa cattgtcact
    37921 tacgtttcca agtgctgcac caaaacgtag ttcatcaaga tatggagcga ctacttctgc
    37981 agcctgacgg gcaataacat aattgatatt cacttgacca ttagagtcac gttcgtccaa
    38041 ttgatctacg aaacgacccc agtatttttc ttcatcaagg gtataaacct tttcttcaac
    38101 ttcaacgtga tcaaattcgt tgtctttgtt acgtttgtag tctttcaact ctgttgtgtt
    38161 accagttgct actgtaaaag agcgaccttg caaggttact gcatcgcttg atgtcacaag
    38221 tggtgttgaa tatgaattta ctgcaagtac atcctcaata atcccaagat gtttcttgcg
    38281 tgattctgct gtgtttaatt cttcaaatgc catttatttt tcctcttttc ttttattaca
    38341 agaagtcttt acgccatttt tccgtgactt cttgctggac tgtttgtgca tttttgatag
    38401 gtgcactacc tttcatacgt tcagaaactc ccttctgaac tgactcttcc catgcttttt
    38461 gtatagaggt aatagattca gataccgtct ctgcgcttgt caaatcgact acatttacta
    38521 actcaacagg taagtcacgt tcacttaaca ttgctttagc ttctgcggtc aattccttgc
    38581 gagcaatagc cttttcacgg tcagctagtt cttgctcacg ctgatccaac tgatatttct
    38641 gcttctcatc agcgttcatc ttagcaagct tcttcgcttc gttttcttta gcttcttgct
    38701 ctgatttcca cttggcaaac ttcttatcga tgatagcgtc gacgtctgcg tccgtgtact
    38761 tcttctcgtc ttgcggttgt tgtgcaggtt ctgcaggtac cttttgttct tcaaccgttt
    38821 cgactgtttg tgtttcttcg ttcattgcga acctcctatt tttaaagtcg tccccgactg
    38881 taatttccat agcttttagt gtcgtcaatg cttggacaat ataaaaaccg tacgggattc
    38941 catacggtta gagcataaga aaaccgcctc gatttcgatg cggttaattt ttatagttta
    39001 atttcttcaa tttttgcacg ttgttctaga attcttaaat aattccacat ggttgaacgc
    39061 tgacctttta acaaatcaat cggacattta gattcaaact ctagttgccc tttttcgtat
    39121 ttcccaatca tcatatctaa cttctggaat cgttctttca attcgtagta ttctttttta
    39181 aatctttctt tccattcttc catttttctg ttcctttctt tacaccttat aattattccg
    39241 ctgattacgg ccataataat aaatattaac aacaaaaata ccaaccaccc aaaggcgatt
    39301 gatacccaat cccatataaa catgtcttta ctcctttcta agcatcattt tttaggctta
    39361 gcattctttt ccacccattt tttgaaatca tcaaacgtat ccatcttttt caataataga
    39421 tacttctcaa cttcttcaat ggcttcctca actttagcgt catgaaaaca gtaaccgtta
    39481 cctgataaat caaaaatttt attttgtttt ttcttatcaa caatccatag ctcctcacca
    39541 tgccaagcac tctgtggatc ataacatttc ttagattgta tctcaagacc gttattttca
    39601 atcaattcta tcaacttttt gtacttattc atcagattct cctttctgag cacgaaaaaa
    39661 gcacttagat ttctctaggt gcttaagtaa taaattgcat ttttatattt tttaacacgc
    39721 tcgtagtctg tattggtaac agatttcaaa cgtgataaat ctgagttatg tttcaaatct
    39781 gcaagtttta caactcttgc taaattattt gattttactt tcccaagata ttcttgataa
    39841 ctttgacctt ttttctttgt caaaatttgt accgctgtaa caacttcatt tgacaagcca
    39901 gacgcgaata aatcggcagc agttatatcg ctatcctcaa tcacatcatg taaaagagcg
    39961 acagcttttt cttgttcagt gttgacttga ctggccacat agagaggatg ctgtatgtaa
    40021 tcaacacccg ctttatctac ctgccctgca tgtgcttttt tagcgatagc caaggcaata
    40081 tcaatcatgc cgctaccatc ctgtcaatat aagtaaatgc atcattttct gaaatttctt
    40141 caaaatccgt aaagtcatta aaaaagattt tattaaacca atccatgcta ttaacccact
    40201 ttttttcaat gtcaaaaact tgcatgacac catcaatcaa acgaagtact tgagcattgt
    40261 tcgtcgttgt gcggtagtat ttaatatctt tcacatcact tcaccctctc tatattttta
    40321 ggaatctcaa gcccattgct taaatcaagc atttccttaa ataatttcat gcgttctaga
    40381 tcagatgtat tcgtatcacg atacttctca tagagttcat gtaatgaacc attttttaag
    40441 tcgaaacttt cctgagtatg atactgcatt tcaaagttga taccatcttt ttcaacgact
    40501 gtattcacac ctttgtatgg tccatctact agccaagtgt tttttacttt tacaatttta
    40561 taaccttctg cgataagctt ctgtttcatc ttcaaatact cttctgtaaa agtatcggaa
    40621 tcgaaaatag ttgtgtacct taaggcgtca ttaatcttac tcacggcttt tgacaaactt
    40681 atattttcaa ctaggctatc tgcaataatt ttacgtgata atgactcaac tgttttcttc
    40741 ctaaattcaa gacctgccaa tttgttttct cctgcgatac gttgcatatc acttgtaatt
    40801 tttggctcaa ctcctgaaat tttggacaat agttgttcgc tataaaattt cgccttggct
    40861 tctcttgtat cttgattata caccttttcc ccgtctttcg caacatactt gctataccac
    40921 tctttataag tcatatcggc aggcacgtac tcaactttac ctgtctctgg attccttgct
    40981 ctgcgcttca acttgctgta gtctgcgtcc tcatcgtatc cgacagtagt agacctacac
    41041 caagggtgca taggcggaca attgacacca gggacagcct tatccctatc atagacctga
    41101 ttgtcatgct cctgacaaat gcgtgatgta cgcttgtcta agacggccac aaagatatac
    41161 ttctctatat ccgcttcctc atagctgagt agctccattt ggttatgaaa aaaggctgat
    41221 tctgtccgaa ccaaacgcct tgcatcgttc tgccccacat tgaacctctc agcaattgct
    41281 tgtgcagttt ctcgtgtatc tcggcctgtc ataaggctta tgagtaattc atcttttatg
    41341 cttgatgtaa gcttccctgt attcttccag atgtttgttg agtacgtact tccgtcacct
    41401 acccaactga aagactgtag atgtttaatc tcgctctcag gaagcccaga aaagccgtat
    41461 gctagtcctg tctgctgctg caggtcaaag gtagccttgt agtaactatc cttcatcagg
    41521 tcgctataaa aggcgtctga gcctgtcttt tctgaatgat agatagattc acgcatacgg
    41581 tctaaatcat cactcaaacg ctctaggcgc ttcatacgga aagaataagc tgggctgtct
    41641 aaatcagcta gtaatctttg gatgttcggg tcattcggtc tcgcttcaag tactttacga
    41701 agttcattca gatttttctt gtctttcatg ttcttcaata cttgtctagc ttctacctga
    41761 cttagaccat aatcacgttg gaacttatca aaaatcttat tgacttcctt atccaagtaa
    41821 gtcttggctt cctgatagac cttatcgaac tggtctgcct gcttttcggc cttgtccatc
    41881 tgctggtaaa tcagattggc tttcctcttc gcccaatact cctgattctt catcctctac
    41941 ctcgtcttcg ggtttcgtgt tgtctttgtt gaacatcggc atgtcttcca tgttcttctt
    42001 tttttcttct tccaaggctt ccagctcagc gtcagggtct tccacaaacg gcaagagaga
    42061 aataagctgt ctattggtca ctttgccttc caagttgttc acaatctgag agatttctaa
    42121 caagttctta ggcaaaccac gactgaattg tggaacgatt gaatgagact ctaaagcaat
    42181 ctgcttcatg cctaagtaat gagcaaaaat cgcaatacgc tgacgcaatc ctcgcttata
    42241 gttcgcttcc ttggtcttag taatcatctc aaggcccatc agcttaaatt ccatggctac
    42301 gcctgatgta ttccctgcga aattctcatc agtcaaatta ggcacatggc taaatgtgta
    42361 gatgtcctct ttaagagctg tacgcaagat ttcagtagca ctttcgtcca gcgtattctt
    42421 caagaactca gcccttgcac tatcgcccgg caattccaaa agaccttctt cagaaagaat
    42481 cttcattgct accttagcgt cttctggagt gtctgctaac tgcgtgccat acaagacaag
    42541 tatagactct acagcctgtt ccttatcatt gacacgattc cccatcaagg aattataagc
    42601 gtctatcaag ctaatctgtt gctcgtagtc gccaatcgca aagtgattat tgcgatactc
    42661 gataattggg atttgaccaa ggttgtgagg tgttgcctcc tcgctctgag ttgttcctga
    42721 atctgtactt ctcagcacca tgtgatagtg cagattttcg gtaaagacct cagcctggta
    42781 cttggtagtg tctttcgtat cgtcttttac ttcatagtaa tagaccgcaa acaaaggctt
    42841 ccgctcaata ctatcatcgt agaccatgaa agtattctcc ggatcaatac tagttgaatc
    42901 caactcagtc aatccttctt tagcatagat gtattcataa gcacgaccat agatagccat
    42961 gttcaaagca ttctgagcat ctacttggtc aatctcagca ccatcaaagg ctgtaagtag
    43021 ttcatcgata tcaccgtcag cagtattgtt atacttgata ggattgccca taaaatagcc
    43081 cgtagccgtg tctgcgatat ccttggcatg attggctacc gtcttgtaat tcggtgcgtt
    43141 cacgttgcgt ctcgtgtgtt ttaagatagc atgctcaccc aaatagtagc ttttaagctt
    43201 cttcaaatgc gagccttcag tgctatgtat cgttatcaat ttgtaaatca ggtctttctt
    43261 caaagaaccc tcatcatatc catcccgtgg ataggttaaa tattggtaca tgtctttcct
    43321 ctctatagac cataatcaga acgtctacgg acagttgctt taggttgaga atgttgtgag
    43381 taaatcgcat aacgcaccgc atccagcacg tcgtcattct ctttcactgg ctcgcccgtc
    43441 ttttcattcc agatatactg ataaacttcg tctttgaact tgctgacctt gtttgataca
    43501 acaaaaaagc gcccagcttt catcagcttg gctacttctt caataccaga caataccgct
    43561 ttattagcgt tgaatgttct taattgctct ctttgaaatc tagcaacgtg ttcaggtcgt
    43621 gcactgtctg cccagaacgt aatattcccg tatcgttcct tgatattctt agcgaggtct
    43681 acccaaaaat ctatctcttt gtactgatga gcgtgttcct ctaacagata agctgaaccg
    43741 ccagatgttt ctccaatgac aacaatagag ccaaagtgtt catatcccca gtcaacacca
    43801 gcatagactt tagtgatatc ttttggtacg ttatctacaa ccatattctc gctaaaatca
    43861 cgatagacga cgccctcacc agtcacccac agaccaagaa tatctcggtc ataaaataca
    43921 ccagctggtg tcgcattctt gatattctct cggtatctgt cagacatgaa tgtattatca
    43981 tctaacttaa aatgaaagtc gatgatcata tcgtctccag agttgatata atcccgtctg
    44041 agccagtgtg tcgggatgtc tgggttgcta tcccaaacaa tcctagcacc ctctcctgag
    44101 caacgtgaga tgatttcctt gaatacttgt tcgttagcaa gagatgcctc gtttacataa
    44161 gctccaaaag cagtaaaacc acgggcgcgt tttaaaccag atatagaacc ggtgtagact
    44221 tgaactacct tgacaccgca aagggtaaaa gctccgtgct tatcgtattt aggttcaata
    44281 tcaaacatgt tatacagttc ctgaatgata ttgttttgta tcgatgttga agatgttcca
    44341 gctaagatat acatcggctc atctatgttt aatctatccg ctgtttctct cactcgtgca
    44401 atctcattca tgaagaccat gttgtttaga acagttttac ctgaacgttt tgcaccatgg
    44461 agaccacaga taaaaaaatc atcattcaat actcgtgtaa gcacttcttc ttgtcgcttt
    44521 gtaaatttat ttgtcatcaa aagcacctct caaagccttg gcaaagtcta tcaatttatc
    44581 gtcttgttca ttatccacac cgatttgtga tttaagtttt tcgatttcaa gttctagttt
    44641 ctcagcttgt ttagcagtcg gataacgctt caatatctca gctatcgctt taataactgt
    44701 gttattatct gcctttttcg taactctatc cacctcacca gtgacagggt tcatcatcaa
    44761 aacttcctca agtcgctggc ctcttgcaat gtctgagaga atcgaaagag cctctttagc
    44821 gctcaaaata ttttcatcgt gcatcttttc agtttcggtt tgtataaacg ttttaacgct
    44881 tgcattttct agcaatttac tagcggttgt tttagcatac gcttcactat aacctgcgaa
    44941 tattgcggat tgatagacat taccagtcct caaatactcg ctcgcaaaca tcttttgtct
    45001 ttgatttaac ccaatgtcca tcaccacctt tcgaataatc aaaaaaagcc acacgatgtg
    45061 cgaccttctt gcaaggcgac tacaagcttg cgtgcgtatt aaattttgac ttctttttta
    45121 ttttttgtag tctttaaaac ctctgaggga atcaaaccct ctagcttata acttatccgg
    45181 aatataatta gctacgcaat catgcgaggt ccagtcgctt ccgcaaccat ttttaagtta
    45241 atgagtgata ggagttaatg agtgatatgt gaatccccac ccagaagatt taactcattc
    45301 tgggacacaa acactcaaag gagaggggaa gacttgaacc tccaaggcca ttacagcccc
    45361 ctgacattac aggtaaccat ctaccaattc tgagacctct cttttcaatt cttgatacta
    45421 ccattctaac agatttttag aaccgtgctg ttccaaaaag tcccataagc tcactatgag
    45481 gttagatgac ttcttccaaa gctaagaccg cctcattttt taacctgtag taggttgtac
    45541 gactcatatt caaatcataa caaacgctat cagcggtgcc tttgttgatg taagtcattc
    45601 ttaatactgc cctgtacttt ggatttttaa gcctattgat cattctacct aattcaagtt
    45661 ttctgttaat gacctcttta gtatcctgct ctatagcctc tttcatcact accaactgag
    45721 tatagacatc atcaactttt ctagtctgtc caccttggac tttgacgtca atccacttgg
    45781 ggcttgagag caaacctgcc tcaagctcgt taatttcgtc tatacggctt tggatgtcca
    45841 tgtccagatc ctgcaactct ttcaagagct ctttagcctt gttcactctc tgtctccttt
    45901 gtgatataat aatagtgttt gaaattattg ctgagacaga gagtgtcttg gcttttttta
    45961 atgcttaaat tcgttgacca ggtcacggat aaagaacttc cagtcagatt ctctaaaagt
    46021 caagaaacga tctgtagtaa gatttctaag ttttttatag aaaagcatct ttagttgaat
    46081 tgactcaccg acactcagta aggtaccagg aaagcgatgt actgaaacca ctctattccc
    46141 gtatccagaa atatctaatt gtattattgt ttctggataa aaacgcccaa ctttagcttc
    46201 aactccgagc tcaactttga cctcttccac aattggaact tcgtttaaaa ttggtcgtgc
    46261 aggaaataat ggcgacgaga ctttttgcct ttttcctgaa tatgggtatc ttttaggttt
    46321 cattgtttat ccccttcttt attctctaaa acggcatctt gtataaaagt attaccgatt
    46381 ttatagtgct tgtattcctc aactgttact tcaaatgttt cttcaacttg cttattacct
    46441 gtaaattctg aaacgaccag aatgtatttt cttttggttc tggttggcac aagtaccgaa
    46501 cttttaccat taataacagg tatgaacgtt gtgtgaggtt catcaatgta cttatctacc
    46561 actgtcccac tcgaaatctg gtgacatgct acgagtaagg atgcgaataa aacaatacat
    46621 aggattttaa aatatctcac tcgttgacct ccaaaaactc tagattttcg tagatgttgc
    46681 caagcacttc agattcatca atttcagacc acaaacgcac tgcgacactg ccagtatcaa
    46741 taacccaaga cccttcaaga acctttacaa ctcctacata atctttctca tattcataga
    46801 aaccgccaat ttcgtcagct ctacccaaaa atctagtagt tcgtacaata tcgccttcaa
    46861 agatttcctt tccgttcttg tctttgagtc ctgttgattg catgagttcg atttcgtcaa
    46921 aatcataaca atagatatct ctatcgtctg gtaaaccatt ctcaaaataa acttgttgtg
    46981 tcactatttc tttgttttcg tagtcaatag caagaatgtc atctgaaaaa accatacgtt
    47041 ttcctatttt tacccacact ctaaattttg gaatcattcc tccacctcct ctatatcaat
    47101 tccttcacaa tcaaacaccc agccgaactt tgcttcttct agttcttttt gagtaccttt
    47161 ataattccta gctgttatat cttgactaaa ataaagagta ctccctgatt gcgatttgac
    47221 caaaggctgc ctatttttta aagtcaccaa ataccgcttc tcttcctcga cctcgtagcc
    47281 gtcaagccaa gcaagacaga atttttcgat gttattttcg taaaaccaat caggaacttt
    47341 cttatcataa tgatcttcaa ttactctcat tgcaccgtaa acatgaaaat tgtttttctt
    47401 tttaaattct atatattccg ccacaaactg cggaattttg actttttggg gttcgtctag
    47461 ttgtttcaac tctttcaaaa aaagttcaac cattgaagta taaggtacag gttcataaaa
    47521 agggctgtgt tcattccaca attcttcata cttcttaatc aatgccttaa tattcatctt
    47581 agtttcctct ataaatcaaa taaactgcaa taactacctg agccatgctt ggcgaatagc
    47641 caacccaatc atcaaactcc ttagattttg gcaaccaatc cttagtagct cccaaatcat
    47701 agtctgtagg cttttcatca gcgaagatgc attccatcgc tcccataaac gtcataccat
    47761 cttctgccat ttcccaaaaa tagtccgccc ggtctttcac cgcttgtggt aaattttgct
    47821 tgggaggttg cggcttcccg tcttctaccg tccagttgta tacttcatta actttttgct
    47881 ttaactcttc catcatcttc caactcctcc gctttccgtc ttaattctca ttatcttcct
    47941 cctcaatttt aataacggcc ctaccgtttg ggtgtcgtct ttggtgtgat gtgtaagtgt
    48001 aatactttaa catcctttca gtaattcctg tctcgctact gatctgcgct aatgttccaa
    48061 gcgtaacaaa cacatcacct tgatataatg cgtagtcagc catctgctcc tcatttcttc
    48121 aaatactcag ggatttcatg acctcaatct caacctctat ccgtggattt agactgtaga
    48181 acttgcctac atcatgtagc gctatctgac cgtcgtcctg gaagacgatc cctgacatgc
    48241 tgtcatatag cgctttttcg tagttatcta tgtcaggctt tttgcctact ggtataattt
    48301 catccaggag ggcctgttgg ttcttcttga ctttagaaat gtactgagga ggtttgatat
    48361 aaaatctaag ccgtgtcctc aaagctccct caaggatagg ctgacccatg tactgattag
    48421 caatgagcag ctggcaatga ttacgccatg ttttcatatc cttgtcttcg taagttgtgg
    48481 taaaactccc acgtcttgca aaccttggcc gtgattgagg tttaggctca atgttcaggg
    48541 tcaatttcat tcaagagccc ccttaaatcc tgccatctca aagagatttt ctctgttttc
    48601 gtttacgaac tcaatgctca tatcatagct tagagtcgta acgacctcta ttttttgttt
    48661 ctcaatgatg atagcaatac gttctgtcac attgatttta cttgccatgt tctttctcct
    48721 gttaaaaaag tgtcgtttgc aaagggtaca catcttcaaa cggtactcca agtcttagac
    48781 agtcttgttt gatgtccatt gtagaaatca catacttgac gccattgttt ttcttgtcgt
    48841 aatgtggaaa agtgtacccg tcattttcaa ttttgatctt gatgtccgtt ttggtttcag
    48901 gtttccaatc cacccaatcc gtccactcca tctcatgcct catcaaataa acttaattga
    48961 gcgttgtggt tttttatccg ctcctctgct ataccaaaat aatattcatc tatttcagtt
    49021 cctataaaat gcctatccgt ttctaacgct gctaatgctg tcgtaccgct tcccatgaaa
    49081 ccatcaaaaa cagtatcccc aacatcagaa tgtttaacaa tacatcgttt tatcaattca
    49141 ataggttttt gattttgatg gagtagttca tctgaactta ctctcttgaa atcccaaaca
    49201 tctgtcagtc gctcaccgtt gaatttcttc cgtcctttgt ttgccaatat aatcatctcg
    49261 tactgttttc caaactgcgc ctctaaatcg ccagcggtat gattattttt tcgccatata
    49321 ataatatttt ttactgaaaa aatacttttc aatttctttt ttaaaaaaat caaccttgtc
    49381 aaatgaacag aatatataaa tagccgtatc atcttttaaa attcgataac actcttttat
    49441 atactctttt atcaattcag gattgttatc atttttgata acatttgaaa atttatgctt
    49501 ttctttccgc caatttgttt tataatttat caaataaggc gggtctgtta cgattaaatc
    49561 aattttatta tcaggaattg tttccataaa ttccaagaca tcttttttta taatcttatc
    49621 tatttccact aactgtccct cctaaaacgg caaaccgtca tttgggaggt caataagacc
    49681 taacttgtaa gcgcccaatg atcccaataa gagagccctt gctgcaatac tgagcaatga
    49741 tgtcagctgt acctctccaa gcttgaaaat tgataaaatc agcctcacgc tctccatttt
    49801 cgtttttgaa attgcgattg actgcaagcg tgccctgtaa actagataca ttattaggcg
    49861 tttttcgtag atcaggaggc gctacaagcc tcccaaccag tgtgacgtta ttgatcatct
    49921 gatttgtccc cctctagtgc tacggtctcc caagagatac cctaaaaaca tccataggat
    49981 agccatccca atctctttga taaaatcatt cattatttct ctcctttgca ttcataacat
    50041 acattttgac ctacatcttt tcccttaatt attgataagc taccacattt ctcacagctg
    50101 attatgaaac ctaaaccatt tgaattaata ctgcttatat tgttctctga gggaactttg
    50161 taaataatca atgcggatgt atgccaatat tcagcactga ctccactgtc agcgacagca
    50221 gacacatttg attgaaattt gatgtcaatc aacttaatgc ctggattttc ggcaagccag
    50281 ctatttattt ggtcgtcaat cgcctcatga tgtggataat cacatgaaaa aaatacggtt
    50341 ttaatcatat tcccctcctg gattgtgcca ccagatcatc aggtcttcct gattatctct
    50401 gatgtactgc tcaaattttt caaagtggac gatagcatgt tttaagcgtt gcataccctc
    50461 tccagatttt gagcaaaagc tgaaaacttt aaagacaggc tcaatcatgt caataatttc
    50521 tacgacttgg ccattgaggt tccagacgct atcctctccc accttaaaat ctaggataaa
    50581 ctcatcccct aggttgtgga taacctgcaa tttcttgccg tccgagtaga tggctacgct
    50641 gtcagatatt tttctgatgt ccatggttac ctccccattg actctggaga aatatccaag
    50701 atttttctat ctccaatttc ctttttttcg ccatacagag tcgctaatag gtcctctatt
    50761 tttcctatta actcatcagg caccccatat tcagccaatt cttctgaaat tttttcaatt
    50821 tctgtcatac ttaccaccca cattgttcat ttagttcagc ctgagttaat ggctcgatac
    50881 gttgataacc cctgacttga tagttctttt taaaatcaaa tccgagttga cttagaccag
    50941 ccttgaaacg gtctttttcg gctgtgtcta caaaatacac ctctaaagtc attttttggg
    51001 catatcgttt taggtcattt tcagcccctc taagagcgtt aggctcattt tggaggattt
    51061 gtccaccgtc caagattttg cccgtttctg ggtcaaaatt tggggtttcc gttgattttg
    51121 gagcctgttc ttgctgtttg gtttgttggg ctgctaaaag ttcctgactt tctcgctctg
    51181 ctcgttcttg agcctgtctg atttcttcct tttgcttttc aaactcataa tcagctttga
    51241 tttgtccaaa gacttcagca agagtcaagt ctttcagctg tctaatgtaa ggtgagtcag
    51301 tcatgccata ctcagcacat aaccctgaaa tagctgactt ggctttttca aattcttgtt
    51361 gtttctgaaa ttcaaatgtg accatgtcat caagtgactt catagtggct tttttaagcg
    51421 tcacgccatc tgccataaaa tcgctagctt tgacatactc aagggccttt tcatcaaaga
    51481 gacgaggatc cagcatgtac tcagctgatt tgttggctag gtagcctttg actgtgtcaa
    51541 ttcggacagc cttttgatgt tcttcaaact ctttgacatc accagcaatt ttggtaatga
    51601 tgtcttttag aggctggatg gcattcttga catacttgtc aaattcatca gctggttcag
    51661 ataagacttt cttattcctg atccgttcat cagaaacctg cttgtctaat tttcgtagat
    51721 cggcaagtgt ctgcttgtca tccttgatag ttgcagccgt aaccgtgtaa ttttgatact
    51781 ttgctacaac ctcattgata ttctgctcaa atttctcacg gtcaatgatt tcaacctgtg
    51841 cttgtgttac ttttacctgt aattcttgca tgttgtcctc ctaatattca agttcaccgt
    51901 ctagcaactc gccctggatt ggatcctcat tttgagtagg ttcaggatct gcatgatttg
    51961 cctcttgctc tttgttgaat tgatcaatct gagccatctt gcgtgctacg acatcctcac
    52021 ggctctcttg aggtgtgacg tctttgattg cgtcaaatgt atctccacca tcgatgtctg
    52081 tatacatgtt cccaagttcg tcagggaaag cctctctaag agtctgcact agagctgttt
    52141 ttctgatcat agtggctggc attgatttcc aagtgctccg tggattgcca tttctgtctg
    52201 ttttgacata ctcattaaag tcaactgtga ccttatatct gtgtgaacgg tctttacgat
    52261 agacaacggc ccaaccacct attagaatgt cctcaggtaa tttaagagag ccctcaatct
    52321 ctatcatttc tccatttctc atgacagtaa ttccagcctc aaaaccatca tatccctcac
    52381 aacgttcagc acgtttcata aatgcctctt ttgagacaat caagctgaac tctgtgccgc
    52441 tattgttttg ataggctaca atgtagacct cgttagcaaa tgggttaaga ttgcgacctt
    52501 tacacaaggc tagagcctga cctacttgtt tttcagtcag taggttttgt gggtcaaaat
    52561 actttttgat gtctgcccca gtcaataaac ttggatcagt agtgatgtca cgttttatct
    52621 gtgttgctaa ttgattatta gtcatcttgt tttctcctat gtgttcatgt caattctgcg
    52681 tctagactta ctatttaagt catctaattc atttttgtaa tcttgaataa gttgcaaatt
    52741 ccggtcaatg aagcgttcta caacttgacc tagaagttct tgtgttgtca cgcctctcaa
    52801 ttcagcaaga agtctgatat attctttttg tttttcagag atctctgctc ttatgaacga
    52861 ctttccttta ttggtcatct gcgtcatttt tttctccttt aggctagcaa tctcctacat
    52921 agatccattg accagcgctg aaaatccaat cagctgggtc aagttcttct cgttcttcag
    52981 gcggttgcat tatatctctg tcataattaa acatgagcat acaccttccc aagctccaga
    53041 actcgtttca catatctagc cttggatgtt agcccaagat ccagcaattc gtttttttct
    53101 tcatgattgg ccaaaagcca gacacggttt tcaatttcaa tctttgtcat cttcctgctc
    53161 cacctcttca attttcactt cgctatttag acgtttcatg gcttcatcta ccgacttgcc
    53221 gcccaggacg tccttgagca tgtggcttac atcgtgcatt gtttgagcct tcgccttgct
    53281 tctttcatcc tctggcatca atccgacatc ttgtagagcc agaaaggcta aactgacatc
    53341 gtgcatttct ttctgaagct gtttgatttt tttgattgtt tttagtgctt taaacatatt
    53401 gttctcccat ctgttctttt tctttgtaga ttgccaattg ttgtttcaga tcatagattt
    53461 cttgctcaca cataaagcga cgtttgcgct cttcgagaag gtcctcatta agctctaccg
    53521 ctactactct ccaatcaagg ctcacttcct tgatgattcc ctcgagtctg agttttaact
    53581 tagtaagtga tttcattaag ctacatcctc cttgttagat cgcttgttca tacctagaat
    53641 gatgtcatag tacgaatgac cagcagggat gacatagcct gtcaagtctt caatgaccga
    53701 accatctgcc attatgttta caattcttgg tttccattgc tctttttttc tcttcatgtt
    53761 ataatttcct tgaataattt tgttgagcgc ctgattgccg tcaggtgctt ttttatttta
    53821 tgtcgtatag acacttccat tcgtcgcata atacgtcagc tcattcatct tattagtgaa
    53881 ccgttcgtct gtcgtaatca gcaacctctc tttaagcagg gttgatagtc cgtaaaattg
    53941 gctttcaaac tgttcaatag cctgcttgcg ttcctcagta gtcagttgct gacaaggagc
    54001 gtctcgaagc tgtgtctttg ctgaacttaa aagcattcgt cttcatattt cctttcgtta
    54061 ttctgtcaac gagactctgc tcgtaaagtt ctttgaggtg cttaccttca aaattagttg
    54121 tgataattgt attcgtcctg ttttcaagta tttgatacag gactttctgc atccagttat
    54181 tgccttgtct gatttcgttc ccaacactcg actctttgcc taggtcgtcc aaaatcaaga
    54241 agtcaacgtt ttgcaggaat ttcacgactg agcgttgttc ccacttagag tccttgtatt
    54301 gaaaagcctc ttgcattcga gaaaacagct ccatggatgg catatagact accgatttcc
    54361 gaacttggag catttgaaag ctctcgttta aggttttagc tatcccgacg gccagatggc
    54421 tcttgccaac tccaggcggt ccagagataa tcgtattccc ttcgtagcgc tctttcacat
    54481 agtcagccgt gactcgctta gcgaaattga ctgctttagc gtcctgattt gtatggattt
    54541 caaaatttcc aacagtcgca tttttcaaat cgtttgggat gatgctctct ttcataaaga
    54601 gagaatatga tctcgtatct ctgatttgag cttcagcaat agctaactgt tcgcttgcgt
    54661 tctggttgat agtctcttga acacattcag gacaataggt cagcgtgttg cgagtgcaag
    54721 ggttgataga ccgccacatg tatacacctt cgtgttttgg acattgctgt ttcaaagtct
    54781 caacctgcaa ggctctttct tgcaactctt tgcttgatac tacttgcatg cgcaccccct
    54841 aaaatccaag ccgtggatca aatccatcat cggataatct caagcgaccg ttcgacttat
    54901 tgtttgaccg agtaggcttc tgcctgttct ctaccagttc aacagtcgtt aaacctttct
    54961 gtttccagtc tctcaatata ctttcaagat atttaaagta aggtttacca ttgcccacac
    55021 attccttgat ggctaacttg ataacctctt tactatggtc ttgtaagaag tatttcaagt
    55081 cctcaatttc aaatggtgtc gggtatcgtc cgaattctga aaaaatccaa tcatgaacaa
    55141 ttcctaagtc gttttctgcg ggggcgtcct ctatactata tagattatta gcacccaccc
    55201 cctctggttc aatcaggttg gttatataag ggtggttatt atcaggttgg ttagacttaa
    55261 tatttttaag ttcttgaact aaaatttctt tagttccccc cttaatattt ttaagttctt
    55321 gaactaaaat ttctttagtt ctgattttct gaggatatag caaattcgcc aatctaacgc
    55381 cctgtctttt ttctttcaaa agaccatgtt ctatcagttg cttttttaat ttgattacaa
    55441 ctggctcgct tttttttatc cacttaccaa tttcttcgtt agtagctaca acgtacagat
    55501 atccatcttt atcagtaaaa tgttgcttat tttttcgaga taatgaaacg cgatcggtca
    55561 aaataccata aaccataaca gccagcggat ctaaatctga aaaataatca tcttctagca
    55621 gccgatacgg gattttaaag tatctttcgt gattatccat gtctatctct gtaaaatatt
    55681 gttcattcat acctctccta aactatgatt taattcgtat tttttgccta aaaaaataaa
    55741 atccttttcg acattgtata gtcgagcaag tttgtctaaa agatccattg gaattttcga
    55801 actatcatgc tcgtacttca acagtgtttg ttgatgaata ttaagtttgt cggcaacttc
    55861 ttttgcagat aaattataat ttgttcttat tgctctcaat gtcattttcg gcacattcct
    55921 acctccttat ttttctattt gttcctcgca attctgctat aataaaagca gaaaggaggt
    55981 aatgttatga ctgattatca attagaagct tctctgattg tccttggcaa agagtacgaa
    56041 agagccaaga aagatggtaa agaaagcttc agtatacatg tgtcgttctt tgatggacta
    56101 gatactaatt tccatcttca agagtttgca agacaatatc ccgtaaggat tgtccgttcg
    56161 aagcctttcc aaataacttt tctaataaag taatatcatt tagagggaag ggattggttt
    56221 caactctatc gttaaacgtt aaaacaactt cacaactctc tagaaaacga ttcgtaaatt
    56281 ccactcgctc tactccgtcc aaaaacattc catcgacgaa tacagcaggg tgggtttttc
    56341 ttgccgtcag cagcacatcg tgctctgatg tatttatcgc aatcgttttt gtcatacctt
    56401 cgtctcactc tctagcgccc tgagttctat ctcatggctg acttgtttca atagcttctc
    56461 acacgctatt ttagcttctc tgtacgttgt gttttcgctg atgaagtaat cagcaagttc
    56521 gatgatttta tcttccatga ccgtctccaa aaatcggtct taagaccgat gtaacccctt
    56581 caaaaacagt atatatttat attatcctta acaagaaagg agctgatgca aattggcaaa
    56641 atttttgaag gggactgtgg ttcagtgatt cagtttggct aggtaaccaa cgcgttttta
    56701 ctgcgagtgt gactgcacgg agcctgtcgc tgactataag agggactgca gctctgctta
    56761 tagcgggact gacagataac taccgagcga cactcaaaga ctagccaaac cacgttgatt
    56821 gcagtgctgg acgcatgacc agcgaagttt caaccagttg ctttacaccg actgtgaaac
    56881 cttatcaaag tgtgcaagtc ttgacctagt gtaaagtagg ttaagacttt ttttattgct
    56941 caggaacttg tgaatcgttc aaagaaatct tagaatctag ttcgtccact ttctcagcaa
    57001 tatatgtcac ggtcctcaat atctcattga gggctgttct ttctaattcg tccatctttc
    57061 ttctcctttc tttttctttt gctctatgag caacaacctg ccaaggattc gaaccttggt
    57121 gataccaatc aggctacatt cattttgtcc tgcattccta caaatgctgc atcgaaacga
    57181 atatcatcga tttcatcttg agtaaaacca gaatcgagaa ggtaacgctc ttggcactcg
    57241 atctcttctg ctaactctgt ccatccgaaa gcgaactgac ggcagttagt acagaaagct
    57301 tcaagtttgc tatagaggaa aatttcctcg taagtgcctt ggattaaagt ttccttagct
    57361 actgctttga agatgttgat tgctttctcg tttaatgtgt tcatggtgtt tccctccgat
    57421 ttgttttttg ttatttcctt aagcttgatt atattatact acgatttaaa tcgtatgtca
    57481 ataacttttt cgaatttttt cgtaattttt tcgaattttt tatttacaaa atcgaaaata
    57541 aacggtatta tatagtaaag aagataggag gaaaaaacat ggcaagagga cgaggaaaat
    57601 taactcctca agataaagag gatatgaaag tcttttccgc aaatcttaac tcaattttat
    57661 ctgatagaaa ttgtaaacaa gctgagctat ctcgagcgac aggaatacca cctagcacat
    57721 tgacagggta tgtaaaagga acttctttgc caatccctgg caatgttcaa aaaattgcag
    57781 atttttttgg agtacctaaa tctgtattag accctagatt tgtaactaat aattctatgg
    57841 tcgatgactc ttcttctaat acttcctcta tccaaaccat ctacgaccaa ctagaaccgc
    57901 ctagacaggg caaagtcctg aattatgcaa agaggcaact gaaagagcag aggaacgaag
    57961 aagaaacgaa gataaacgaa gtatcggaga acatcatcag actggacgac tacagacaga
    58021 ctacttaccg acgtgttact ggggttgtct ctgctggtag tggttcgata caggacgacg
    58081 atttagatat ggaggtttcg ttctatgagg atgaaatccc agacgactac gacgctatcg
    58141 cttatgtcgt cggcaactct atggagccaa agataaagaa tggtgactat ctttttataa
    58201 agaataccca acaggttgat tataacacta tcggcatctt ccaagtagac ggcgctaact
    58261 atgttaagaa actgcgtcag ggatatctgg aaagcttgaa tccagattat gaggatatac
    58321 acctagacga aagcaacgac atccgaacta ttggggaagt tgtcagtgtg tatagagaga
    58381 aataaaatga gtaaagaaaa tccatatttt gaacaaacca aacaaaacta catagaagtt
    58441 gaaaaactct ataaacttgg taaagcaaaa catacatctt ctaaataccg atttcttgca
    58501 ccagcagtta aaagacaatc tgaacaattc ttgtttgaag ctaagactca aaaaagaaaa
    58561 tattggaaat tcagtcgtgg ctctctgata ttcgtagagt tcggtgtaaa tataggcgga
    58621 gaattatcaa ataaccattg ggctattgtc ttagacaagg tagatagtcc ctataaaaaa
    58681 acactcacag taattcctct aacatctaaa aatcaaatag ataccgtact catagacgaa
    58741 atcattgcgg aatatccttc tattttgctt gatgaatata ttgaaaaatt acacaaagaa
    58801 ttatttgcct acctaaaata tttagattcc aataatgcaa ttactgaagc tgccttatcg
    58861 gatgtctacc aagcttatac agagcaattt tcaaacgaaa taattcaacc taagatagta
    58921 gacgatgaca accttaaacg gacacaatca gaaataactg acattattga attaactcaa
    58981 tactacaaaa aatacattaa gcgttcttat gccaagtgta ataaccttca aacaatcagc
    59041 aaagatagaa ttttaaagaa aaatagatta gatccaatcg gaaaaatgaa agtatctgat
    59101 aacacattgg acaaaattaa cgaaaagtta aaagaattat accttttcta atctcttgac
    59161 atttcttaat aattatatta caatacagct attaggagtt tagctccata aagtttacat
    59221 ttggatttta gatccataac gtgatggtag ccgtatttga tacggctact tttcttttta
    59281 tctagcaact gtttccattt tggaaacaac tcaaaaaagc cccacgctca gaagtttggc
    59341 gaccgagagc gtgaggctag gagcaagaaa aaagcattaa aaagctgttt ttcttgtacc
    59401 tattttatca aaaaaggggt acaaattcaa tgaaaacaat gaataaagtg gctatatatg
    59461 tcagggtttc gacgacaaac caggttgagg agggatactc tatagatgag caaaaagaca
    59521 agctctctag ctactgcgac attaaagact ggaatgtata caaagtatat actgatggag
    59581 gtttctcagg atccaatact gacagaccag cgctagaaag tcttatcaaa gacgctaaaa
    59641 aaagaaaatt tgacacagtt ctagtctata agctggaccg tcttagccgt agtcaaaaag
    59701 acacgcttca cttgattgag gatgtattca tcaagaatgg gattgaattt ctgagcttgc
    59761 aggagaactt tgacacctct actccttttg gtaaggctat gattggactc ttgagcgtct
    59821 ttgctcagct agaaagggag caaatcaagg aacgcatgca acttggcaag ctaggacgtg
    59881 ccaaatctgg taaatccatg atgtgggcta agacatccta tggttacgat taccacaaag
    59941 agacaggcac agtgaccatc aatccagctc aggctctgac cattaagttt atctttgaga
    60001 gttacctgag agggagatct attactaagt tgagagatga tctaaatgag aaatacccaa
    60061 agcatgtgcc ttggagctat cgggcggtca gaaccatact cgataaccct gtctattgtg
    60121 gtttcaatca gtataaggga gaaatttatc caggtaatca tgagccgatt atttcaaaag
    60181 aggaatacga taagactcaa tctgagctaa aaataagaca aagaacagca gcagagaatg
    60241 tcaatcctag accattccaa gctaagtaca ttttatccgg tatcgcccaa tgtggatatt
    60301 gtggcgctcc tttaaaaatt atgttaggcg taaagaggaa agatgggagc aggttaaaaa
    60361 aatatgaatg ccaccaaagg cacccacgaa cgctgagagg cgttactacc tacaacgaca
    60421 ataaaaagtg tgactcagga ttttactaca aagacaagct agaggcctct gtgctaaaag
    60481 aaataagcaa actacaagat gacgctgatt acctggacaa aatattttca ggagacaatg
    60541 ctgagaccat agaccgtgag agctataaga aacaaataga ggagctatca aagaaactga
    60601 gcagacttaa cgatctatac atagatgacc gcattaccct tgaagaatta cagagcaagt
    60661 cagccgaatt tataagcatg agggggactc ttgaaactga actagaaaac gatccagcac
    60721 tcaggaagaa caaaagaaag gctgatatga ggaaactgct aaacgctgag aaagtctttt
    60781 caatggacta cgaaaatcaa aaggtgcttg ttagaaggct tataaacaag gttaaggtga
    60841 cagctgagga cattgttatc aattggaaaa tataaataat tttagtaacc tacatttcca
    60901 ccaatgtaaa agctttaacc ttagcttttt tcaagaatgt catcattttt ttcattttaa
    60961 aaatttacct ccatattttg atacatgggc atgagcattg ccgcataaag taaaacgata
    61021 atcagtgcca caaagataaa aaccagtggc tgcaccaaat tcatggtgcg gttgactcgg
    61081 gtaaaaaagg cttcccaagt tttttcagca tagatttcca actcactacc cagcttggac
    61141 ttaacttccc catactctat gatgagactc aattccttcc taaagaaagg ataggttcct
    61201 atcgtctgag aaaattcacg gccatttttc agggtttgag ccagatcttg accgacttct
    61261 ttaaagagct gggaaccttg ttcctgcatc atttgaaaaa tctgcgtcaa ctccattccc
    61321 tgtgaaatca tattccccca ttcacgtgca taataggctg tcaagtaggt ctgcacaaag
    61381 attccaataa agggaaggcg tgctaagata gaaaagacac tcatcttaga acttctttta
    61441 taaaaagtga gtgctaaaag ggcaagcacg gaaacaagcc ctaccatgcc tagaaaaatt
    61501 tggggcagat taccgataat ttgggtggca atattgctac tatccagttg tgggagcagg
    61561 taattccgta gccccagcat aattaagaga agaaaaccca gcaaaatcaa gggataggtc
    61621 gctacttcaa tcaatttttt cttgacctta gccagattgt ccagatattc ttctatcttt
    61681 cccaaactca ggtggagatt gccatgaact tcagctaggg ataactgagt gacaatagca
    61741 cttgaacatc ccaaactttc catcatttct gagaatgatt tcccctgaga caagcccaca
    61801 cgcatctggg tcacacactg cttgtccaac aaagcactcc tatctaaaaa ggagatagtc
    61861 tccaccagat gaaaaccgct agaaaagaga ttgttaaata gggtgatgat atttttttgc
    61921 ttagctgtag ctaatttttt ccgtctcagc ctgaagactt gtgatatgtc catctttaag
    61981 aagctggtca atttgctcat tccacttggc tgcttggtgt tcttgataat ctctgcttgc
    62041 aaagtcaacg attcctcctc ccccgattaa tctctggtag cagactcctt gcagaacaac
    62101 tgccaattct tcttcactca cacccaactc cagcagacgc tcataaacac ctcggatact
    62161 cttggcgtga atggttgaaa agactgtcgc acctgtcaaa ctagctctga ccactgcacg
    62221 cgccgtctcg ctgtcacgaa tttctccgat aatcaagaga tctggtcgat gacgcaagga
    62281 aagtttgatt agattttcat aggttaggcc gattgcttcg ttcaactgca actgaagcat
    62341 gtcgtcctgc ttgatttcga caggatcttc gatggacata acttgctgtc ctttaaagag
    62401 tgacttggac aattcatgca tcaaggtcgt cttaccactc ccaaccggac cagcaaaaag
    62461 atagagtccc cgttgcctgt actgcttgcc taattcttca atatcctgaa accaaaaatg
    62521 caggtcctgc tcctcatcgt gcaacaaacg gataaccaaa ctctcatgcc cccgataatc
    62581 gcctacagta gataaacgta gagacgctat cttatggtca taggcataat cacaggaacc
    62641 cagttgacta cgtctttttt ctcccacatt catacccgcc acaaacttaa agtgactgat
    62701 aacggctgca aacttttcaa aatcatagct accaatttta cagcgctcgt ctcctaccct
    62761 catatgaagc tcataggcgt ctaacttagg gacaaaatag atatcctgcg tccctttttt
    62821 ccgagctgaa cgaatgattt cttgtgcaat ttcttgaacc atacttacct cctcacctat
    62881 actattcgca aagagttaga aaaataaaaa aagcaactcc aaaaaagtta cttttctcta
    62941 ttttaatttc atacggcaag aacgattcct ttcctgactg gtttgttttt catagcctga
    63001 gcgtagtttt tcgtctggga taacctgctc atcctgcaaa agagccaaag aatggtattg
    63061 ttgtaaatac tcatcacatt catcacacca gcgttggtct tccgggtccc aaaaaatggt
    63121 catcaagtcg cttctacttt tataaagagg cgattcttgt tccaatctaa accagcaagt
    63181 tttgtagtat ttgagagcat cataatactg gtcaaatttt ctacttgcta caatatcttc
    63241 ttcccaacct tctaagaacc accacggttc aaaatctccg tacatttcta taacacgata
    63301 catattcatt cattcctttg taccgtatat tataaccaat tccgccagac aaggaaagaa
    63361 atatgctcat ttcttaattt tttgataaaa aatccagcca tctgatggat gactgaattt
    63421 ctatttactt atgtacaaaa atatttttac tacttaggct tctgagatat cgttttcgat
    63481 aatgacctta atggcatggt ggtctgctgc cttactgaag acttcgtagg ctttttcaat
    63541 ttcactgagt ttgaaatagt gagttaccaa tttttccggt tcaatcttat gactttcaag
    63601 tgctttcaac aattgtggag tcgtatttgt agataccaaa ccagttgtta cattgatgtt
    63661 gcgaatccaa agtttatcta aatcgaattc aactggttta ccatgcacac cacagttggc
    63721 aaccgttccg tctacaccga taatcttttg acagaaatca aatgttgcag gaataccaac
    63781 agcttcgata gcgacatcca caccacgacc atctgtcaaa tcataaattt ctttaatggc
    63841 tttttcaggg tctgaagaat taaccttatg agtcgcaccg aatgataggg cagtttccaa
    63901 gcggttatcg tctaggtcta ccataatcaa tttagctggt gaatagaatt gggctgttaa
    63961 aagagcagcc aatccaactg gacctgaacc aataatggct acgctgcaac caggttctac
    64021 tttccctttt aagacaccaa tttcatatcc agtaggcaga atgtctgaca gcataaccaa
    64081 agcttcatct gacaagtctt ctggagtatg gtaaagagta ttatctgcat gagggacacg
    64141 tagatattca gcctgcatac catcaatcaa gtgaccgaaa atccagcccc cttcgtcttc
    64201 acagtgagca taaattcctt ttttacagta gtagcattta ccacaggcac agacgcaaga
    64261 aatcaagacc ttgtcacctt ttttgaagtt ggaaactcct tccccaactt cttcaacaat
    64321 cccaatccct tcgtggccaa gaatggtacc actttggcaa gtaggaacat cccctttgat
    64381 aatatggagg tctgttccac aaatagtggt ttttacaata cgcacaatag cgtctgttgg
    64441 cttgcgaata actggtttgt ctacatcaac aaaagaagca agtcctggtt taacataagt
    64501 ataggctttc ataaagcact cctccgtttt tttatttgta atatttatag ttcaattgta
    64561 agcctttttc tttgtttctt caagcttttt ttgtgatttt tttaactttt tatttaccag
    64621 taaagtaccc tgcataaaaa agcagaagtt agtctctaac ttctgctttc tctcttatgc
    64681 ttgataacgt tttacgccat ctaggtaggt tgctaccaat tccaaatctt tatctaatac
    64741 gatgaagtca gcgtcgtagc cttcacggat ttggccacag acgtcatcga tgtgaacgga
    64801 tttagctggg ttgaagctgg ccatcatgac tgcttcatgc ggattcgcaa ttccccattc
    64861 gaccacattc ttcaaaccat ctttgagttt gaggatagaa cctgccaagt tacctgtcga
    64921 tttgaggcgt gcagttccat ttgcaacgac aactgggaat tctcccaaca tataatctcc
    64981 gtcttccaat cccccagctg tcatacagtc tgtgataaga gcgatgtttt ctgttccttt
    65041 ttgtttgata agaatttcgc aagcctttgg atctacgtgg tgaccatcac agatcaactc
    65101 tgcataggta tgtggcaatt ggtacatggc tccaaccatc cccaattcac ggtgagtcaa
    65161 cccacgcatt ccattgtagg catgcaccca aacactcgct ccagcatcga ctgctttttt
    65221 ggcttcatca aaagtcgcgt ttgaatgtcc aagagcaacc gtcacacctt cgcccgtaac
    65281 tgtacgaaca aagtcttcca ccccatcacg ttctggtgca agggcaattt tattaagcaa
    65341 gccatttgcc gctttttgcc aagaatgaaa ctcctcaaca cccgggtctc tcatataagt
    65401 tggattttgt gcccccttaa aagtttctgt gaaatatgga ccttcataat aaatcccacg
    65461 aatcttagca cctgttgctt ctttataatg gtttccaaga ttttcagtga ctgcaagcaa
    65521 ttgctcataa gtggctgtta aagttgtggg taagaaactg gtaacaccgg tactaagaag
    65581 tccttcactc atagtatgca atgtaccttc aatgttgttg tccatcacat ctacacctgc
    65641 atatccatga atatgagtat ccacaagacc tggggcaatg ctataacctg tatagtcaat
    65701 cacctcagcc ccttcaggaa tctgctctac atgtttccca aacttgccgt ccacaagttc
    65761 caagtaacca cctcgacgaa ctccgtgtgg gtagaaaaac tgatccgctt taatatagtt
    65821 aggcataatg ttaacctcct taaaagattg attctacaat ttattatgtc aattacatta
    65881 taaacctttc ttttttattt gtaaatggta tagacctatt ttttggagat attttaaaag
    65941 agctggattt ctccaactct tctctttttt aatacaagtt attttgattt aaccggcttg
    66001 tcttgagctg tctgcaaagc tgtggcaatc gtatctgcat acaattttgc tcctgcttcg
    66061 atagtgctac tctcactccc gaaatgaacc tggtctgttc cagcccaaat ttctggatgc
    66121 tctttcgcaa cttgattcca atctgctatc gtaatgtaag gtgtcttctc tgccaattct
    66181 ctcatatagg cagcagcctt ctcaacgatg gcataggtct cttttgtctt atctccctca
    66241 taaggagtca ccaaaatcat atggtgtccc ttaggaagat ttttcacgat actgtcccag
    66301 tcatccttgt aattctcagg attatttacc ccagtcgcaa tgaccaccgt cttaggtaaa
    66361 aatttattct ggctattatt tagcatgatt tcatttgcgg tcttggttgt tacgctgacc
    66421 tgcgcgttaa tctgtgctcc aggaagagct gtctgtagtg ctgtatttgc ccttaaagcc
    66481 actgagtcac caattaacat agtgccatca gcaattccca aactgtttgc atctgcccgt
    66541 tctgccatca ccttggtctg gccaatattt gttgcagctt gcttcaagcc attgacagtc
    66601 aagtctgtct caaacgctcc cacttgtggt gccaacaagg tcaccgtgca gacaatgatg
    66661 gtcaagattc ctgtacctgc tgcaagaatt gcgtgaatat aaggcagggg acgaagggtt
    66721 tggacaatag gtgtgttctt gcctgcaatc caaggttcca atacataaaa tgacagactg
    66781 gcaaagccat aagaacaaat cagagtcagt aatacagcaa gaagatttga tgtcaactgt
    66841 gagaaaatga tatagaaagg ccaatggaaa agataaaccg catagctagt atccgctaaa
    66901 aagctgataa tcttcgactc ctgtatatga tgtgtctttt catgtaagac acgcgccgcc
    66961 agaatcatgg caagagctgc aagactggca agtaagaagc cgataagata ggcaaaaaga
    67021 taagtgaatt tgacaaagaa agtcaaaaga actaggaaac caaagcctcc tccaaaaact
    67081 accaaagtct ttcgtaaatc ccagatttta tccaactgct tgacgaggga agtcgtctga
    67141 cgaacgccta caatagttgc taacatactt cctaaaaaga atggatagac atgagttaaa
    67201 ctggagaaat aaacagagga ataagaggtc actagaaaac taccaataaa catggagaag
    67261 aaactgatca agaaggcaac agcagataag agaaagacca tccccttcaa ctgaccattt
    67321 gatttagctt gtttggataa gaaccaaact gccaatcccc aaagaatata gtagtgaacc
    67381 tcaactgcca aactccaatt atgaacaaac aaatgaggaa tgaactgaga ttcataactc
    67441 ccacctgtta ggagttcata gaagttggtc ataaagccta agacgcccgc aatctggcca
    67501 ccaattccag caacatagtc ttggcgaacc aagaaagtaa aaggcatggt caccaagacc
    67561 atcaaaacca caggtggcac aatccgataa aagcgtcttc taaaaaatcc tatcaaatca
    67621 atctcattgt tcttagaaaa ttcttcaatg agtagagctg taatcaggaa acctgaaaat
    67681 gtgaaaaaga catctacccc gaaaaatcct ccaggaaaga tcgtctgaaa gaagtgatac
    67741 aagagtacca aaagtaaacc tataatccta atcaaggaaa accatttaat gcgcatacga
    67801 gtttattctt cctttcattg tacactttat tctatcaaaa aaagaagaaa aatcctaaga
    67861 gaaaacttag gattttttta tatcaattcc attttagaaa ttacgtcctg acttattata
    67921 gccatatttt tccacaaaat actcacggaa ttccaagaga ttgtcatcca tgatggcttg
    67981 tcgcacttgc ttcatcaggt taagcaagaa gtaaagattg tggtagctag tcaagcggat
    68041 accaaaggtt tcatcagcct tgagcaggtg acgaaggtaa gcgcgtgtat agttattaca
    68101 tgtgtagcaa tcacactcag gatccagtgg cgtaaagtcc tcagcaaact gggcattttt
    68161 cacaaccaaa cgtccttgac tggtcataca agtcccgtta cgagcaattc gagtcggtaa
    68221 gacacagtca aacatatcca ccccacgaat gaccccatcg atcaagctat ctggcgctcc
    68281 cacacccatc agataacgag gtttattttc aggcagcagt tgagttgtaa agtccaagac
    68341 cgcattcatc tcttcatggg tttctcccac tgccaaacca ccgatagagt agcctgagaa
    68401 atccatgctg acaagatcat gagctgattg gcggcgaagg tcttcaaatc ctgcaccttg
    68461 cacaattcca aacaaacctt ggtcatgtgg acgacggtga gccttcaaac cacgctcagc
    68521 ccaacggctg gtacgctcga tcgatttctt aacgtagtca taaggttgat aaaactgagg
    68581 acattcatca aaggacatca tgatgtctga acccagatta ttctgaatag agatggcttt
    68641 ttctggggat aggaacatct tagaaccatt tagatgattt ttaaaggtta ctccttcttc
    68701 tgtgatatta cggctatctg ctaaagaata aacctgaaaa ccaccactat ctgtcaagat
    68761 aggctggtcc caattcatga acttgtggag accaccagcg cgtgcaatga gttcatctcc
    68821 agggcgaagc cagagatgat aggtgtttga taggataatt cccgaaccca tctccttcaa
    68881 ttcttcaggt gactgagttt tgacagtggc ttgtgtccca actggcataa acataggtgt
    68941 cggaaaggta ccgtggggag tgatgatttc tcccagacga gctcctgtgt gtttttcttt
    69001 cttaatcaaa cgatatttga ttggtgaatc tgacattttt tacctccgaa gctgggaaag
    69061 acagtcccag ttcatacagt gtgcccaagg acacactgta tgattttatc aaaaaaagta
    69121 ggaactgtca cgaactatgt ataagaacta tggtataata agagaatgaa ctatccaaaa
    69181 attgatttaa aaacaattcg tcaggagtca aaacattttc aggctgacac cccccgtctc
    69241 tttctcctct atatccttcc cagtatgctg gtcatcttat ctggttttct taatccctta
    69301 tctcggatcc acgggactgt tttagaacag ccatttttca gcatacttgg ccaaatactt
    69361 caaacctacc tctttcctct actagtctcc tttatcggga ctattctttt gaccagttcc
    69421 gtctatgcaa cactgacact catgaaagat tctaaaacgg agccatctgt aaaaaacagc
    69481 ctagctctct ttgatgagga gcgcttttct caaacctttt tgaccctact tctcaaacgt
    69541 ttctatctct ttttatggag tatccctaat ttacttggga tttacctcct cttttatagt
    69601 agctttttag ccaaaaaatt cgtcacccta catcctgagt ttccaaatct agatctctca
    69661 tcagttgaaa ccgagcgttt cctcatggtc tttggactct actttcttgc aagtctcatc
    69721 ttgatcattg taggaaatat cctctatatt ccacagtatt atgcctattc acaggtggaa
    69781 tttcttctct gttatagtct agacctagga caggtcccac caagacgaat cctaaaaact
    69841 agccgttcct ttatgaaggg ctacaagttc cagcactttg tcctagattt acagcttctt
    69901 ccttggtact tcctcaattg gattaccttt ggcattgcta gtttttcact cctaccttat
    69961 atccaatgca ctaaaattat gttttaccga gcagtattgg cccgaaaacg tccaaaagct
    70021 tgaaggtttt tcccctcaag cttttttcta tcaatgagtt tctccgccta ccaacttgag
    70081 ttcctgatct ccatgttcta ttatagcgcc gattgctgct tctatccctc gccgaatatc
    70141 cactaaactc atagctggag tagtcggtct gttcaccacc tgttccatca tataaggaat
    70201 atgcataaaa cctgccttaa catatggaga tttcttttct accaaataga gagcctgata
    70261 catcaaatgg ctgcagacaa aagtccctgc cgtattggaa acagaggccg gtaagccctc
    70321 tttttttata gcttgaacca tcgctttaat cggcaaacta ctaaagtagg ccgaagcacc
    70381 atctgggcga atgggacggt caatcggttg attatcttcg ttatcagaaa tgcatgcatc
    70441 gtcttgatta atggtcactc gttcaggtgt caaactagtt cttccaccag cttgcccaat
    70501 acaaaggaca aagtcaggtt gataacgatt catctcttct tccaatactt gagcagattt
    70561 gtgaaaaact gtcggcacct ctagccaacg gacctcagca ccatggattt cagctggtaa
    70621 acctttaatg gcctccaaag ctggattgcc cttttcccct ccaaagggct caaaacctgt
    70681 cactaatact ttcatttcta actcctcaaa accaattcaa tgagactttt ttcttaaaaa
    70741 caagtataac atatttccct tattctggat taaaaaggat tagcgagatt agaatcgacc
    70801 ttcaagtgta tttaaagagt aaaatactaa tcaaggccaa aatagctgat ccaccttgtt
    70861 tcaaaataat ttttttatcc gctgttaaag agccgtaagt cgcagcacca atcacaaata
    70921 agacaaaaat agtcacaatt tctaaattct gtgagaaata aatgacatac aagagaaaga
    70981 ctcctagcag agccttataa attccttgat ttttgaacaa tgaacttact gacggatgag
    71041 ccaattcttc cttttccata ttaaatacac gactagtcgc atctgattgc gtggcaatac
    71101 tttccaaata aaaaatgtaa aaatgctcca aagcaacgat cgttgttaaa ataattgaca
    71161 tctatttctc cttaaatttc tatattttca atacccaaaa ccaatttatt taataaacgg
    71221 ctgagttctg ttctctcaaa ctctgttaag atactttcta tctcttcctt aaccctgata
    71281 tgttgcagag ggggattcac cactaactgc tccttggcat acttcgcagc ctctaccaac
    71341 acttcccgct gattttcagg attacgatga cgctccacca aaccttccgt ttccaaaatt
    71401 ttgaaatgcc gtgtcaaagc agcctgatca attttcaaac gctcctgaac cgccatttgg
    71461 ttacaaggag aatgctccag caaaaacagt aaaatctgat accgtgtcaa actaatccca
    71521 agcctttttt caaacaattg cgtactcgct tgctcagaca agcggagttg atacagtaaa
    71581 tcttgaatct ctagcatttc ttcccctctc tttttattga tttatcaata tttgactaat
    71641 taattgtgat ataaagtaaa atagattgca agaatgttgc ttaactcatt acaagatgat
    71701 aactttccat tttccattca aattgatact caatgaaaat caaaaagcaa actaggaagc
    71761 tagtcgcagg ttgctcaaag cacagctttg aagtttcaga taaaactgac gtggtttgaa
    71821 gagattttcg aagagtatga caaagaaaaa acgaacgaga atcgctttcc atcacgaaag
    71881 ctagatctca ttcgtcaaaa tgatgttatc aagtcgggtt tatccattca ttgaaacgtg
    71941 aacgtgatca tagtgatttt ctgtcacact accacggtct ggcattgggt tccaagtgtt
    72001 agctggccca tatttgctat cgaatggagc atagaaacgt tgtttccaga tgatgtaact
    72061 aatgccacgg ctggccatat tttgaatagc atattccgca atcttatccc ctaattctga
    72121 acgttctggt accataaagt cgatagccaa accttttccg tgatctccac tgtctcctgg
    72181 acgataacca ctaaaggatg taatgccaaa caagttagca atttcttctt taaaggcagc
    72241 tgtttgtggt tgaagacctg cattttcaga ttttgctact gcaagtccag cataatcggg
    72301 cgcagctgga gcctcgtaag ttgttgaaat tattttcgtc tcttctggtt gataagtaga
    72361 aactgctgct gtagctccat ttgatgatgc tacttctttt gcttctgaac ttgttgtagt
    72421 tacttccact gctgatgcag cttgagggct tgcttgtgtt tcctgcttct cagctggagt
    72481 cgtttcctga ggagttgctt cttcaactgg gcgagttgtt tcggtcgttt gctcctctgg
    72541 gacagaagtg cccgtagatg gtgccacttc ttcagaagca atcactgtct ttgtaacttc
    72601 tgcaattggt tgagaaaggt ctgcaacctg aacagtttga tcatcaacgg tcacttgatt
    72661 agtggtcaaa tctgctgtcg cagttgtcac ttcttcacta gagtctgctt gaggtgtttg
    72721 gatttcaact tctgttactt cttctgcttc attgacagtc gttgtcaaaa cagtttctgg
    72781 gaaaatcaag tccatattag tgattttgtt cagattcgca agcactgtga catctacacc
    72841 caaggcttct gcaatggtgc tcaaagtatc accatactgt acggtataac ttgttttgtt
    72901 gtccgtttta gtcaaatcgt tttggatttg ctcaacacta cgtgcagtcc aaagaacttc
    72961 ttctgcttga gttgccaata ctggggcaaa tgacaaggct actgttgacg ctaataacat
    73021 tcttttcttc attctttcaa attcctttca aatgagtacc tgtctatcat aacacaaaaa
    73081 atgtagaaaa aaagccctct cgggcctatt taacagaact gtctattcct tgtaacaaac
    73141 taggttactt gaaatagttt gttaggtctc tgtcacaaaa ctgacacaat ctttttatca
    73201 cttagtctca aaaatacggg aaatatctgc taacgcttga atcctgtgat tcccttcata
    73261 agtagactct aaaaagttga tgctttgaat cccactattc tgggcaaatt ccacatccag
    73321 agtccgatcc cctatataat aagtattatc agaattcaac tgatacttat ctagcagata
    73381 ggtagccgct tctggacttg gcttccgcac aaagccactc tgactggtta aaatctctgt
    73441 aaaataggat tccaccccca agtccttgag aatggtaaaa gcgttgttcc ccttatgagt
    73501 atatataaac tgctgaattc ctgattcgtc tgcccaagct agcacctcac gcgcacctgg
    73561 catcaaaact acctgagcat tcttctcagc cagactctgg gcacgcacct gatttagcac
    73621 ctcaacatcc agatttctat cttctgccac ccgcacaagc aaatcttgca ccgaatactt
    73681 gaagataaac tctctcacct tctccttatc ataaggaata gaaaactgag caaaagtctc
    73741 ctcaatccct gataaaatcg cttcgtaaga gtccaataaa gtcccgtcta aatcccaaat
    73801 aaaagctgtt ttttgcattt cctatccttt caagctcata tagcgctggt aacgatagcg
    73861 tagaaataac cagcgaaaac cattatccaa gagagaccct gcccaaatac caggcaagcc
    73921 ccaaccaagc acaatcccca tcagatatcc tgtcccaatg cggatacacc acattcctat
    73981 acttgtcgca taaaaaggga ggcgtgcatt tcctaatccc tgccagactg ccgtatagat
    74041 gactgttcct gtcgtcatag gggtcccaag tagtgaaaac agtgtcacta gaacactagc
    74101 ctccaccgct agagaatcag tcgtatagag atgagttaat ggtacaccca agacatatat
    74161 actaaaggac aggggcaaca tgaggaacag agaaagccaa aaggtttgtt tactcaaact
    74221 agcaactctt ttccaatcat cctctccaac tgctcgggcc aacagcatga ccgttgccgt
    74281 agcgacgcca aaggcaggca tatagttaaa ctgggtcaag acttctccga ttgcattccc
    74341 agcaactgcc tccgtcccaa aagaaacgac caaggcaatg atcactacat ctccagccct
    74401 catcataagt cgctctccag ctgctggtaa agccaaggtc aacagttcct tatctaaacc
    74461 aaaagttggc ttcccataag gcagttttaa ttgtgaccac aaaatcacaa gaccaaccaa
    74521 acgagacaca attgtccccc aagcaacacc agctatcccc atatccagaa caaaaatagc
    74581 tagacttgaa aaaagaatat tcaaggcatt ggataaaaaa ctaacataga gaggcagacg
    74641 tggattatgc gttgcacgaa tcaaggctcc tagactagtc attaaaccta agagaacaat
    74701 cgatccgcct accaaagata gatacagtcc accactctca gctacatccc tctccgtccc
    74761 caaaagtcct atcatctctt tcccagcgaa gatggacaaa aatcctaaaa ggaaacttaa
    74821 tagtaaggta atcttcaacg cctcagtcac atgataggcc aacttcgact ggtctttctg
    74881 ccctatgctt tttgaaataa cactggaaat agcagctccc agagcgatga aaatcgcctg
    74941 ataaatggtg ataatattac cagctactga aacccctgaa atagctatca atcctaaatg
    75001 agcaaccaaa taactgtcca ccattcccat tagcatctgc aaaaagtttt cacccatagc
    75061 tggcaatgca atattaagaa tgtctttatt tttcttaaac aatctctcct cctgatgaaa
    75121 agaaactcag ttggtttccc aaccgagttt gctccctctg tcttaaagtc ctaaataagc
    75181 ctcaaccgct gcttgcatgt cagcagctgc cactgttgtc ttgtgacgaa ttggagctat
    75241 ttcaagccca tcaactgctg gtggcactgc aacgcctgag atttcatgta attgagccaa
    75301 ggcttcaaag tctgttaaac ctgcttttcc agttacagct tctactgcaa ctactgggaa
    75361 cttgtatgga ctagctgttg aagcaatcac tgtcttagtt acatctccag tggccgattg
    75421 gtatttttta taaactgctg aagcaacagc tgtatgaggg tcctcgatat aagaatctaa
    75481 ctcacaaaca cgcttgatct ctgctgccgt ttcttcctca gtcgcatatt cagctgcaaa
    75541 gaggtccaaa atctctgcat caaagtctgt caacttatat tgtccttgcg tgttcaaggc
    75601 attcataagt tcagttgtct tttcagcatt atttcccaaa agatggaaaa tcaagcgctc
    75661 caagtttgaa gataccaaga tatccataga tgggctggtt gttaccttaa actcacgttt
    75721 tttgtcatag acacgtgttt taaagaagtc tgtcaaaaca ttgttgtcat ttgaagcaca
    75781 gattaattta ccaactggca aaccaatttg tttggcataa aaggcagcca agatatttcc
    75841 aaagtttcct gttggtactg tgaagttaac cttttcacca gctacaattt caccagtctt
    75901 aaccaattga gcgtaagcat aaacataata aacaatttgt ggcaccagac gaccaatgtt
    75961 catagagtta gctgatgaaa attgcaactt gttggtagtc aatttttcac gaagagccac
    76021 gtcgttaaac atgtgcttca catttgtttg cgcatcgtca aagttaccat caatagcaat
    76081 aacatgagta ttgtcgccag tctgagtggt catttgtaac tcttgaatct tgctgacacc
    76141 atcctttgga taaaagacga taatctcagt accaggcaca ttcgcaaacc ccgccatagc
    76201 agctttcccc gtgtcaccag atgtcgctgt caagataaca atcttgttct ccaaaccatg
    76261 tttcttagca gcagtcgtca taaagtatgg caaaatagac aaggccatat ccttaaaggc
    76321 aatcgttgaa ccatggaaaa gttccaaatt gtattgccca tctaatttca ctaatggtgc
    76381 aatagctgga gtatcaaatt tgctatcgta ggcattgttg atacagtagt ccaactcctc
    76441 aactgtaaag tcatctaaaa atgctgacaa aactagctta gcaacttcct ggtaagaagc
    76501 atctttcaat ttgtcaaagt tcaaatctac ctttggataa gtatccggtg taaacaaacc
    76561 gccgtccgtc gccaaacctt gcaaaattgc ttggctggca gttactgtat tgttggcatc
    76621 acgcgttgat tgataaacta atgtcattac tctctatcct ttatctatag tctcgtccat
    76681 tatatcatga tttttcagaa aattctccct ttataagaga aattataggc tcaattcttc
    76741 tctcaaaggc tgtaaatagg tcatttcttc cttatttctc accactagtc cctcctcagc
    76801 tagtaggagt aagtctttgg aaaattcaat aatcgtagtt tcttcctcat ctgtaagatt
    76861 tttcttagaa aattgtctcc ttaaagactt gtaatcataa ccaaatactt taaagaaagg
    76921 tgctgtttct aagtaagctt ctaacttgtc taaattaacc aataatccca agtgaaaagc
    76981 tgcagaagca aaagtcctat caagtggctg tgtacacaca ctacgaaact caactgttcc
    77041 tcgagtcgtt aaatcttggt actggtaact acgatgagtt tcaaaatcct tctcttgggg
    77101 gtaaataata acctcatccc cattcagagc aaatgcttgg atttcggacg tagccaaata
    77161 gtccccagcc tgaataggat aaaaataata ggtctgccca tcacgttccg cagtaaaaat
    77221 cgcagaatga tttagatagt caaaaaaatc agtttcatca ttaaggagtc tagcattgac
    77281 cccaacattc tctggataga taccatgcat agattcttcc cagaaaatat cccttgaaat
    77341 tttcgtatcc caatccgcac ccgaaaattc agagtttgca aataaataag ccttagccgc
    77401 ttcaatttga gtaaaagcat taatcacccg taagtagttg gtttttgaaa tatccagctg
    77461 aacctggctc ccacagataa aagtaccata ttcagggaaa tgatgtaaat ctgatttaat
    77521 aatatttcta ctcaaattca aataatccat caacatctga tagcgtggat aagccactgg
    77581 acaattctca tttttatccc agttgggatg gataccacag ccaacaatag catgatttga
    77641 ttcagctaac tttctctgaa ttacattcat ataattattg aaacgatttt cgacctcttg
    77701 aatcgtttca gccttaccaa atgcaaactc aatcgttgta taggaaactt caaataaaat
    77761 agcatcctga cttatcggat ctactaactg gatcagattg ccaaaatcat ctacctttgc
    77821 tacggtgaga tccaaagtag aaactaaata atgaaataga tccttcataa cttcaacatc
    77881 tgtagcatcc ccttctaaac ttgcaacagg atactccaac tcaattccga caaacaattc
    77941 aggattttct ttaatatttt tcaaataccg atgttttaat aaatcgatag aacaagacat
    78001 actcccctac actttcttaa tctaaataaa atttgaataa ctataattat accaaaaata
    78061 atataaaaaa ggaacgaaga ataacaacgt tcctaacact atactatacc ggcggccggg
    78121 gtcgaaccgg cacgtccttg cggacactgg attttgagtc cagcgcgtct gccaattccg
    78181 ccacgccggc aaatagtaac tggggtagct ggattcgaac caacgcatga gggagtcaaa
    78241 gtcccttgcc ttaccgcttg gctatacccc aatataaaat aggcgagtga tggggatcga
    78301 acccacgcat gccagagcca caatctggtg tgttaaccac ttcaccacac ccgccataat
    78361 tctattaaca cgggcagtag gaattgaacc cacactgaag gttttggaga ccttagttct
    78421 acctttaaac tatgcccgta aaatggaagg ggagggattc gaacccccga acccgaagga
    78481 gcggatttac agtccgccgc gtttagcctc ttcgctaccc ttccaaaata tataaatggc
    78541 gcgagacgga atcgaaccgc cgacacatgg agcttcaatc cattgctcta ccaactgagc
    78601 taccgagcct tattgcggga gcaggatttg aacctacgac cttcgggtta tgagcccgac
    78661 gagctaccga gctgctccat cccgcgttaa tataaaagga ggatgtggga ttcgaaccca
    78721 cgcacgcttt tacacgcctg acggttttca agaccgttcc cttcagccgg acttgggtaa
    78781 tcctccaaaa tagtttatac gggaacaagc ccgtgtcctc ttagtgaaca gataatattc
    78841 caatccccta gtaatggact aaacactatg ggcacgagtg gactcgaacc accgacctca
    78901 cgcttatcag gcgtgcgctc taaccacctg agctacgcgc ccaagtcaaa aacttggtac
    78961 aaagaacaaa gttcaaagcg ggtgacgaga atcgaactcg cgacaacagc ttggaaggct
    79021 gtagttttac cactaaacta caccctctaa a
//
LOCUS       DAVHEW010000002        63038 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVHEW010000002 DAVHEW010000000
VERSION     DAVHEW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.002"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            253..1383
                     /gene="ugpC"
                     /locus_tag="VVL53_000111"
     CDS             253..1383
                     /gene="ugpC"
                     /locus_tag="VVL53_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU4182335.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(1530..1676)
                     /locus_tag="VVL53_000112"
     CDS             complement(1530..1676)
                     /locus_tag="VVL53_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182336.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            1884..2642
                     /locus_tag="VVL53_000113"
     CDS             1884..2642
                     /locus_tag="VVL53_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4182337.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            2729..3241
                     /locus_tag="VVL53_000114"
     CDS             2729..3241
                     /locus_tag="VVL53_000114"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU4182338.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            3423..4367
                     /locus_tag="VVL53_000115"
     CDS             3423..4367
                     /locus_tag="VVL53_000115"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU4182339.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            4376..5053
                     /locus_tag="VVL53_000116"
     CDS             4376..5053
                     /locus_tag="VVL53_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU4182340.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            5151..5909
                     /locus_tag="VVL53_000117"
     CDS             5151..5909
                     /locus_tag="VVL53_000117"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU4182341.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            5947..7452
                     /gene="lytC"
                     /locus_tag="VVL53_000118"
     CDS             5947..7452
                     /gene="lytC"
                     /locus_tag="VVL53_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU4182342.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWYGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            7948..8466
                     /locus_tag="VVL53_000119"
     CDS             7948..8466
                     /locus_tag="VVL53_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU4182343.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            8596..9102
                     /locus_tag="VVL53_000120"
     CDS             8596..9102
                     /locus_tag="VVL53_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU4182344.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            9102..9272
                     /locus_tag="VVL53_000121"
     CDS             9102..9272
                     /locus_tag="VVL53_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182345.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            9304..10536
                     /gene="clpX"
                     /locus_tag="VVL53_000122"
     CDS             9304..10536
                     /gene="clpX"
                     /locus_tag="VVL53_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU4182346.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            10545..11132
                     /locus_tag="VVL53_000123"
     CDS             10545..11132
                     /locus_tag="VVL53_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU4182347.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            11143..11523
                     /locus_tag="VVL53_000124"
     CDS             11143..11523
                     /locus_tag="VVL53_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU4182348.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            11575..12465
                     /gene="rapZ"
                     /locus_tag="VVL53_000125"
     CDS             11575..12465
                     /gene="rapZ"
                     /locus_tag="VVL53_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU4182349.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKCLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            12462..13439
                     /locus_tag="VVL53_000126"
     CDS             12462..13439
                     /locus_tag="VVL53_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU4182350.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            13436..14347
                     /gene="whiA"
                     /locus_tag="VVL53_000127"
     CDS             13436..14347
                     /gene="whiA"
                     /locus_tag="VVL53_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU4182351.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHYQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(14381..15346)
                     /locus_tag="VVL53_000128"
     CDS             complement(14381..15346)
                     /locus_tag="VVL53_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4182352.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            15482..16339
                     /locus_tag="VVL53_000129"
     CDS             15482..16339
                     /locus_tag="VVL53_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU4182353.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            16326..17105
                     /locus_tag="VVL53_000130"
     CDS             16326..17105
                     /locus_tag="VVL53_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU4182354.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            17129..18481
                     /locus_tag="VVL53_000131"
     CDS             17129..18481
                     /locus_tag="VVL53_000131"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU4182355.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            18555..18929
                     /locus_tag="VVL53_000132"
     CDS             18555..18929
                     /locus_tag="VVL53_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU4182356.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            18931..19779
                     /locus_tag="VVL53_000133"
     CDS             18931..19779
                     /locus_tag="VVL53_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU4182357.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNDPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            20045..20812
                     /locus_tag="VVL53_000134"
     CDS             20045..20812
                     /locus_tag="VVL53_000134"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU4182358.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            20824..22008
                     /locus_tag="VVL53_000135"
     CDS             20824..22008
                     /locus_tag="VVL53_000135"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU4182359.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            22005..23876
                     /locus_tag="VVL53_000136"
     CDS             22005..23876
                     /locus_tag="VVL53_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4182360.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDATLLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPIAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(24019..25203)
                     /gene="mntE"
                     /locus_tag="VVL53_000137"
     CDS             complement(24019..25203)
                     /gene="mntE"
                     /locus_tag="VVL53_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU4182361.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDILRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            25507..28203
                     /locus_tag="VVL53_000138"
     CDS             25507..28203
                     /locus_tag="VVL53_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU4182362.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALITFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            28216..29007
                     /gene="yghU"
                     /locus_tag="VVL53_000139"
     CDS             28216..29007
                     /gene="yghU"
                     /locus_tag="VVL53_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU4182363.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            29123..29533
                     /locus_tag="VVL53_000140"
     CDS             29123..29533
                     /locus_tag="VVL53_000140"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4182364.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            29545..31140
                     /locus_tag="VVL53_000141"
     CDS             29545..31140
                     /locus_tag="VVL53_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182365.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            31207..31371
                     /locus_tag="VVL53_000142"
     CDS             31207..31371
                     /locus_tag="VVL53_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU4182366.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            31450..32178
                     /gene="yaaA"
                     /locus_tag="VVL53_000143"
     CDS             31450..32178
                     /gene="yaaA"
                     /locus_tag="VVL53_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU4182367.1"
                     /translation="MKILIPTAKEMNTDFPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPVLSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDETLKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(32455..33003)
                     /locus_tag="VVL53_000144"
     CDS             complement(32455..33003)
                     /locus_tag="VVL53_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4182368.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            33324..33812
                     /locus_tag="VVL53_000145"
     CDS             33324..33812
                     /locus_tag="VVL53_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU4182369.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISSSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            33809..34996
                     /locus_tag="VVL53_000146"
     CDS             33809..34996
                     /locus_tag="VVL53_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4182370.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            34989..35213
                     /locus_tag="VVL53_000147"
     CDS             34989..35213
                     /locus_tag="VVL53_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU4182371.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            35370..36716
                     /gene="asnS"
                     /locus_tag="VVL53_000148"
     CDS             35370..36716
                     /gene="asnS"
                     /locus_tag="VVL53_000148"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU4182372.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPVAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            36869..37159
                     /locus_tag="VVL53_000149"
     CDS             36869..37159
                     /locus_tag="VVL53_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU4182373.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            37171..37641
                     /gene="ssbA"
                     /locus_tag="VVL53_000150"
     CDS             37171..37641
                     /gene="ssbA"
                     /locus_tag="VVL53_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU4182374.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVLDFSRNENPFGATNPLDISDDDLPF"
     gene            37673..37912
                     /locus_tag="VVL53_000151"
     CDS             37673..37912
                     /locus_tag="VVL53_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU4182375.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            38207..39607
                     /locus_tag="VVL53_000152"
     CDS             38207..39607
                     /locus_tag="VVL53_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU4182376.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGNPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            39609..39968
                     /locus_tag="VVL53_000153"
     CDS             39609..39968
                     /locus_tag="VVL53_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU4182377.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            40022..40771
                     /locus_tag="VVL53_000154"
     CDS             40022..40771
                     /locus_tag="VVL53_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU4182378.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            40761..41060
                     /locus_tag="VVL53_000155"
     CDS             40761..41060
                     /locus_tag="VVL53_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU4182379.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            41131..42066
                     /locus_tag="VVL53_000156"
     CDS             41131..42066
                     /locus_tag="VVL53_000156"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU4182380.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            42153..42665
                     /locus_tag="VVL53_000157"
     CDS             42153..42665
                     /locus_tag="VVL53_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU4182381.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDEEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            42690..43298
                     /locus_tag="VVL53_000158"
     CDS             42690..43298
                     /locus_tag="VVL53_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU4182382.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            43399..43581
                     /locus_tag="VVL53_000159"
     CDS             43399..43581
                     /locus_tag="VVL53_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU4182383.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(43627..45072)
                     /locus_tag="VVL53_000160"
     CDS             complement(43627..45072)
                     /locus_tag="VVL53_000160"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU4182384.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            45178..46800
                     /locus_tag="VVL53_000161"
     CDS             45178..46800
                     /locus_tag="VVL53_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU4182385.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            47003..48961
                     /locus_tag="VVL53_000162"
     CDS             47003..48961
                     /locus_tag="VVL53_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4182386.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            49133..49366
                     /locus_tag="VVL53_000163"
     CDS             49133..49366
                     /locus_tag="VVL53_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU4182387.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            49372..50814
                     /locus_tag="VVL53_000164"
     CDS             49372..50814
                     /locus_tag="VVL53_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182388.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRITSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDIICSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            50972..52066
                     /locus_tag="VVL53_000165"
     CDS             50972..52066
                     /locus_tag="VVL53_000165"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU4182389.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            52074..53240
                     /locus_tag="VVL53_000166"
     CDS             52074..53240
                     /locus_tag="VVL53_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4182390.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            53333..56431
                     /locus_tag="VVL53_000167"
     CDS             53333..56431
                     /locus_tag="VVL53_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4182391.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKTDLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADNHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVIYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            56479..57096
                     /locus_tag="VVL53_000168"
     CDS             56479..57096
                     /locus_tag="VVL53_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU4182392.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            57107..58441
                     /locus_tag="VVL53_000169"
     CDS             57107..58441
                     /locus_tag="VVL53_000169"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU4182393.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            58451..58915
                     /locus_tag="VVL53_000170"
     CDS             58451..58915
                     /locus_tag="VVL53_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182394.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            58912..59406
                     /locus_tag="VVL53_000171"
     CDS             58912..59406
                     /locus_tag="VVL53_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182395.1"
                     /translation="MKIRKARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            59487..61142
                     /gene="mltG"
                     /locus_tag="VVL53_000172"
     CDS             59487..61142
                     /gene="mltG"
                     /locus_tag="VVL53_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU4182396.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            61213..61695
                     /gene="greA"
                     /locus_tag="VVL53_000173"
     CDS             61213..61695
                     /gene="greA"
                     /locus_tag="VVL53_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU4182397.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            62203..62601
                     /locus_tag="VVL53_000174"
     CDS             62203..62601
                     /locus_tag="VVL53_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182398.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT        18779 a        11747 c        14295 g        18217 t
ORIGIN      
        1 gctccataat atccataggg gatttaccca ctacaaatat tatagagccc gaaaatatgg
       61 gaaaactgat ccttgtttct gcttttgtct atagaagaat aataaagatt atcttcttca
      121 aattctccga tattctctaa agttttgtgc aagttgcaca gaacttgttt atttttttgg
      181 tcatcttgcc atagaaatat aaagcgtttt catatataat ataattatca aaagacaaaa
      241 ggagttcacc tcatggtaga attgaatctt aaaaatattt acaaaaaata tccaaacagc
      301 gaacactatt cagttgaaga tttcaacttg aacatcaaag ataaagaatt tatcgttttc
      361 gtaggacctt caggatgtgg taaatcaact acactccgta tgattgctgg tcttgaagac
      421 attacagaag gtactgcatc tatcgatggc gtagttgtca acgacgtagc tccaaaagac
      481 cgtgatatcg ccatggtatt ccaaaactac gctctttacc cacacatgac tgtttatgac
      541 aacatggctt tcggtttgaa attgcgtaaa tacagcaaag aagacattaa caaacgtgtt
      601 caagaagcag ctgaaatact tggattgaaa gaattcttgg aacgtaaacc agctgacctt
      661 tcaggtggtc aacgtcaacg tgttgccatg gggcgtgcga ttgtccgtga tgcgaaagta
      721 ttcttgatgg acgaaccttt gtcaaacttg gatgccaaac ttcgtgtatc aatgcgtgct
      781 gaaatcgcta aaattcaccg tcgtatcgga gctacaacta tctatgtaac tcacgaccaa
      841 acagaagcga tgacacttgc agaccgtatc gttattatgt cagctactaa gaaccctgct
      901 ggtacaggta ctatcggacg tgtagaacaa atcggtactc ctcaagaagt ttacaaaaat
      961 ccagttaaca aattcgttgc aggattcatc ggaagcccag ctatgaactt catcaccgtg
     1021 aaattggttg gtagcgaaat tgtttctgac ggtttccgtt tgaaagtgcc agaaggagca
     1081 ttgaaagttc ttcgtgaaaa aggctacgaa ggaaaagaat tgatctttgg tatccgtcca
     1141 gaagacgtga atgcagaacc tgctttcctt gaaacattcc cagactgtgt tgtaaaagcg
     1201 actatctctg tatcagaact gcttggttca gaatctcacc tttactgtca agttggtaaa
     1261 gacgagtttg ttgcaaaagt tgatgctcgt gactacttgc aaacaggtgc aacagttgag
     1321 cttggatttg acttgaacaa agcacacttc ttcgatgtag aaactgaaaa aacaatctac
     1381 taaaataaat aaaattcaaa gcactacaag aaaagatatc tctttatcaa ttgtagtgga
     1441 gagatatcag ttaatctagg gagagaaaca aaatgcttct ctcctttttg ctagagaagt
     1501 catattatgc atctatattg tgatgctctt taatactctt cgaaaatctc ttcaaaccac
     1561 gtcaacgtcg ccttgccgta cgtatgatta ctgatttcgt cagttttatc tgcaacctca
     1621 aagatgtact ttgagcagct tacggctagt ttcctagttt gctctttgat ttccattgag
     1681 tattatttgt gggtaccatc tacaagtgaa gctatatgcg taaactacgt gagcaattga
     1741 attcgaacta gagaggtaat aataaattta tgctatagtt atggtgactt gtatgctttt
     1801 gattctagtt tatcaaataa tagattagaa ttgtcagata atatcatttt gtgttataat
     1861 gaagaaaaaa cagaggtgtt caaatgtcag aagcaggtca taagttttta gcaaaattgg
     1921 ggaaaaaacg cttacgtcca ggtggaaagc gtgccacaga ttggttaatt gcagaaggag
     1981 gattttcaaa agaaaagaga atactagagg ttgcgtgtaa taggggaact acagcaattg
     2041 agttggcaca gcgttttggt tgcaagataa ctgctgttga tatggatgct caagctttag
     2101 aagtggctaa aaaatctgct ggaacggcag gtgttgctca tttaatcagt tttgaaagag
     2161 caaatgcaat gaaacttcct tatcaagatg ctagttttga tattgttata aatgaagcta
     2221 tgctgactat gcaagccgat caagctaaga aaaaatgtgt aatggaatat ctaagggtat
     2281 taaaacctgg aggtcttctc ttgacacatg atgtgcttct taaggaagct aaagagtcta
     2341 tcagacagga attatcacaa gcaattcatg taaatgtagg tcctttaact caagatggtt
     2401 gggaacaggt gatgatagaa tcaggttatt gtgatgtgaa agcattgact ggtgaaatga
     2461 cattaatgaa attatcgggt atgatttatg acgaaggttt gctaggaact ttgaaaattt
     2521 gtgtaaatgc ttgtaaaaag gagaatagaa agcagttttt aactatgtat aaaatgtttg
     2581 ctaagaataa acagaaattg ggctttattg cgatggctag ttataaatcg tcaaaacgtt
     2641 agataattat tgaagttaac ttttcctttt ttctttctta aaaaatatgc tataatagag
     2701 agtaaaaaac tttgaaagaa agaaaaagat gaatttaaaa gattacattg caacaattga
     2761 aaattatcca aaggaaggca ttaccttccg tgatattagt cctttgatgg ctgatggaaa
     2821 tgcttatagc tacgctgttc gtgaaatcgt tcagtatgct actgacaaga aagtcgacat
     2881 gatcgtggga cctgaagctc gtggatttat cgtgggttgt ccagttgcct ttgagttggg
     2941 aattggtttt gcgcctgttc gtaagccagg taaattgcca cgcgaagtta tttctgctga
     3001 ctatgaaaaa gagtacggtg tcgatacctt gactatgcac gcggatgcca ttaagccagg
     3061 tcaacgtgtt cttattgtag atgacctttt ggcgacaggt ggaactgtta aggcaactat
     3121 cgagatgatt gaaaaacttg gtggtgttat ggcaggttgt gccttccttg ttgaattgga
     3181 tgaattgaac ggccgtgaaa aaattggtga ctacgactac aaagttctta tgcattatta
     3241 atgaaaacag tccctagggc tgttttctct acactaggat ataaaaatag actataacta
     3301 gttagagaaa aactataatt gaaaactata tcttcttgca gtataataaa aggactaagt
     3361 gtttgagatt tgtcttcaaa catatgcaat tattcctgaa agagtacagt taggagaggg
     3421 ttatgccgat tcgaattgat aaaaaattgc cagctgttga gattttacgg acagagaata
     3481 tctttgtcat ggatgatcaa cgtgctgccc accaagatat ccgtcctttg aagattttaa
     3541 ttttaaatct catgccacag aaaatggtca cagagaccca gttgttgcgc cacttggcta
     3601 atacacccct acaactggat attgattttc tctatatgga gagccaccgt tctaaaacaa
     3661 ctcgttcaga gcacatggag accttctata aaacttttcc tgaagtcaag gatgagtatt
     3721 ttgatgggat gatcatcacg ggtgctccag ttgagcattt accatttgag gaagtggact
     3781 attgggagga atttagacag atgcttgagt ggtctaagac tcatgtctat tcgacccttc
     3841 atatctgttg gggggctcag gctgggcttt atctgcgcta tggtgtagaa aaataccaga
     3901 tggacagtaa gctatcaggt atttatcctc aggacaccct aaaagagggt caccttctat
     3961 ttagaggctt tgatgatagc tatgtatccc ctcattcacg gcacacggag atttctaagg
     4021 aagaggtctt aaacaagacc aatctcgaga ttttatcaga aggacctcag gttggggttt
     4081 ctattttggc cagtcgtgat ttacgagaaa tttatagttt tggtcatttg gagtatgacc
     4141 gtgatacctt ggcaaaagag tattttcgag atcgtgatgc aggttttgac ccacatattc
     4201 cagaaaatta ctttaaagat gatgatgtta atcaggtacc ttgtctttgt tggtcttcat
     4261 ctgcagccct ctttttcagt aattgggtaa accatgcggt ctatcaggag acgccttttg
     4321 attggagaaa gatagaagat gatgcatctg catatgggta tttataagag gaattatgac
     4381 atatttagac gcttttaaat caggtacctt ggttttaccg agtgccctgc tcttgcattt
     4441 taaggaactc tttccttcta gcgacgattt tctggtttgg caatttttct atttgcaaaa
     4501 tacgacaggc ttagaagaaa tgtcgccaag ccagattgct gaaaggattg gcaaggaaat
     4561 ttcggatgtc aaccagtcca tttctaatct gacggaaagg ggactgctcc agtatcgtac
     4621 tatcgaatta aatggcgaaa ttgaattgct ctttgatgct agtttggcct tggaacgttt
     4681 ggatgacctg tttggagcag ttcattcaag ttcagaccag ctaacacctc aaaaccagct
     4741 caaggatttg gtggaaacct tccagcagga gttgggacga ttgttgacgc cttttgagat
     4801 tgaggatttg accaagacac taaaggaaga tggaaccagt gctgacttga ttaaggaggc
     4861 tcttcgtgaa gctgttttga atggaaaacc aaactggaag tacattcagg cgattttgag
     4921 aaactggcgc catgaaggaa tcaagagtgt ggctcaaatt gaggccaaga gagcagaaag
     4981 agaagcaagc aatcctcagt tgacacaggt atctgcagat ttcataaatg ccatggatct
     5041 ctggaaggat taatccatgc aagtaggctt gaaatccgag taagatttgc aagctgtgta
     5101 taattgtgat agaataaata gaaaataaat tgaaaaaaga ggtatgtgaa atgtcacgta
     5161 aaccatttat cgctggtaac tggaaaatga acaaaaatcc agaagaagct aaagcattcg
     5221 ttgaagcagt tgcatcaaaa cttccttcat cagatcttgt tgaagcaggt atcgctgctc
     5281 cagctcttga tttgacaact gttcttgctg ttgcaaaagg ctcaaacctt aaagttgctg
     5341 ctcaaaactg ctactttgaa aatgcaggtg ctttcactgg tgaaactagc ccacaagttt
     5401 tgaaagaaat cggtactgac tacgttgtta tcggtcactc agaacgccgt gactacttcc
     5461 atgaaactga tgaagatatc aacaaaaaag caaaagcaat ctttgcgaac ggtatgcttc
     5521 caatcatctg ttgtggcgaa tcacttgaaa cttacgaagc tggtaaagct gctgaattcg
     5581 taggtgctca agtatctgct gcattggctg gattgactgc tgaacaagtt gctgcctcag
     5641 ttatcgctta tgagccaatc tgggctatcg gtactggtaa atcagcttca caagatgatg
     5701 cacaaaaaat gtgtaaagtt gttcgtgacg ttgtagctgc tgactttggt caagaagtcg
     5761 cagacaaagt tcgtgttcaa tacggtggtt ctgttaaacc tgaaaatgtt gcttcataca
     5821 tggcttgccc agacgttgac ggtgcccttg taggtggtgc gtcacttgaa gctgaaagct
     5881 tcttggcttt gcttgacttt gtaaaataat cagtaagtag caaaagctag gtggaacagc
     5941 attcagatgt ctgttacatt ttttatagga gaggaaagat tgaaaacaaa aattggatta
     6001 gcaagtatct gtttactagg cttggcaact agtcatgtcg ctgcaaatga aactgaagta
     6061 gcaaaaactt cgcaggatac aacgacagct tcaagtagtt cagagcaaaa tcagtcttct
     6121 aataaaacgc aaacgagcac agaagtacag actaatgctg ctgcccactg gtatggggat
     6181 tattatgtaa aggatgatgg ctctaaagct caaagtgaat ggatttttga caactactat
     6241 aaggcctggt tttatattaa ttcagatggt cgctactcgc agaatgaatg gcatggaaat
     6301 tactacctga aatcaggtgg atatatggcc caaaacgagt ggatctatga cagtaattac
     6361 aagagttggt tttatctcaa gtcagatggg gcttatgctc atcaagaatg gcaattgatt
     6421 ggaaataagt ggtactactt caagaagtgg ggttacatgg ctaaaagcca atggcaagga
     6481 agttatttct tgaatggtca aggagctatg atgcaaaatg aatggctcta tgatccagcc
     6541 tattctgctt atttttatct aaaatccgat ggaacttatg ctaaccaaga gtggcaaaaa
     6601 gtgggcggca aatggtacta tttcaagaag tggggctata tggctcggaa tgagtggcaa
     6661 ggcaactact atttgactgg aagtggtgct atggcgactg acgaagtgat tatggatggt
     6721 gctcgctata tctttgcggc ctctggtgag ctcaaagaaa agaaagattt gaatgtcggc
     6781 tgggttcaca gagatggtaa acgctatttc tttaataata gagaagaaca agtgggaacc
     6841 gaacatgcta agaaaatcat tgatattagt gagcacaatg gtcgtatcaa tgattggaaa
     6901 aaggttattg atgagaacga ggtggatggt gtcattgttc gtctaggtta tagcggtaaa
     6961 gaagacaagg aattggcgca taacattaag gagttaaacc gtctgggaat tccttatggt
     7021 gtctatctct atacctatgc tgaaaatgag accgatgctg agaatgacgc taaacagacc
     7081 attgaactta taaagaaata caatatgaac ctgtcttacc ctatctatta tgatgttgag
     7141 aactgggaat atgtaaataa gagcaagaga gctccaagtg atacagacac ttgggttaaa
     7201 atcatcaaca agtacatgga cacgatgaag caggcgggtt atcaaaatgt gtatgtctat
     7261 agctatcgta gtttattaca gacgcgttta aaacacccag atattttaaa acatgtaaac
     7321 tgggtagcgg cctatacgaa tgctttagaa tgggaaaacc cttattattc aggagaaaaa
     7381 ggttggcaat atacctcttc tgaatacatg aaaggaatcc aagggcgcgt agatgtcagc
     7441 gtttggtatt aagcgatgat ttgaaagagg gatgtgatag tagcaccctc tttttctttg
     7501 ttttatgata gttcatcctc gagtaaattc aagatcttgc ttggaaatga agcttatata
     7561 gtagattgaa tatagacaaa taccttgtga ttggtaaaac attttagaaa ttcatttacc
     7621 tttcctaatc gacttggttt catcttattt caatttatta tagtattggg gaatttcttc
     7681 aaaccacatc agcttggtca gttctacctg cgacctcaaa acttgtgctt tggtcaaact
     7741 gggtttagtt tcctagtttg ctgatggatt tccattgact ataagcatcc aaccctcttt
     7801 ttgtcttcta aagaattctt aaattatcag tctattgcaa cttttctcat ataagttctt
     7861 tgtcttgcta ttggttttcc ttagtagtat actaaggtag taatcattaa gaagtggtta
     7921 caaaaaataa tgaatgaggt aaagaaaatg gtagaattga aaaaagaagc agtaaaagac
     7981 gtaacatcat tgacaaaagc agcgccagta gcattggcaa aaacaaagga agtcttgaac
     8041 caagctgttg ctgatttgta tgtagctcac gttgctttgc accaagtgca ctggtatatg
     8101 catggtcgtg gtttccttgt atggcatcca aaaatggatg agtacatgga agctcttgac
     8161 ggtcaattgg atgaaatcag tgaacgcttg attacactcg gtggaagccc attctctaca
     8221 ttgacagagt tccttcaaaa tagtgaaatc gaagaagaag ctggtgaata ccgtaatgtt
     8281 gaagaaagct tggaacgtgt tcttgttatc taccgttact tgtcagaact tttccaaaaa
     8341 ggtttggatg tcactgatga agaaggtgac gatgtgacaa acggtatctt tgcaggcgct
     8401 aaaactgaaa cagataaaac aatttggatg cttgcagccg aacttggaca agcacctggt
     8461 ttgtaagaaa taaggaaaca tagataaatc tgtaggaaat ttcttacaga ttttttctat
     8521 tttgtaagct attccaaacc agtctttttt tgagtttttt gataaaatag tactatcagt
     8581 gaaaaggatg gaagcatgac taagaaaatc gtagctattt gggcccagga tgaagagggt
     8641 gtgattggta aggaaaatcg tctgccttgg catttgccag cagaattgca gcactttaaa
     8701 gaaacaactc tgaatcatgc tatcttgatg gggcgtgtga cctttgatgg gatggggcgt
     8761 cgcttgcttc caaaacggga aaccttgatt ttgacgcgta atccggaaga aaagatagat
     8821 ggggttgcta cttttcagga cgtccagtct gttcttgact ggtatcaggc tcaagaaaag
     8881 aatctctaca ttatcggtgg gaagcaaatt tttcaggctt ttgaacctta ccttgatgaa
     8941 gtgattgtca ctcacattca tgctcgggtg gaaggagata cctatttccc tgaagaactt
     9001 gacttgtctc tttttgagac agtttcaagc aaattttacg ccaaagatga gaagaatcct
     9061 tatgatttta ccatccaata ccgcaagaga aaggaagtct aatggaacgg agtttatttg
     9121 gtctattcac ggcttttctg tgtttcatct gctttctggc aggagcacaa gcttttcgta
     9181 aaaagcgtta cgggctttct atcctccttt ggttaaatgc ctttaccaat ctggtcaata
     9241 gtattcatgc tttttacatg accttatttt agatagaatg acagtataga atggaaggaa
     9301 atcatgtcta caaatagaaa aaatgatatg atggtttatt gctcattttg tggcaaaaac
     9361 caagaagaag tacaaaaaat aattgctggc aacaatgctt ttatttgtaa tgaatgcgtg
     9421 gagttagctc aggaaatcat tcgagaagaa ttggttgagg aagtcttggc agacttgtct
     9481 gaggtgccaa aaccaattga actcctccat atcttgaacc actatgtaat tggtcaagat
     9541 cgtgccaagc gtgccttggc agtggcggtt tataaccact acaaacgcat caatttccac
     9601 gatacacgcg aagagtcaga agatgtggat ttgcagaagt caaacatttt gatgattggc
     9661 ccaactggtt cagggaaaac tttccttgcc cagaccttgg ctaagagctt gaatgtacct
     9721 tttgctattg cggatgcgac agctctgacg gaggctggtt atgtgggtga ggatgtggaa
     9781 aatatcctcc tcaaactctt gcaggttgct gactttaaca tcgaacgtgc agagcgtggc
     9841 attatctatg tggatgaaat tgacaagatt gccaagaaga gtgagaatgt gtctatcaca
     9901 cgtgatgttt ctggtgaagg ggtgcaacaa gcccttctca agattatcga gggaactgtt
     9961 gctagcgtgc cgcctcaagg tggacgcaaa catccacaac aagagatgat tcaagtggat
    10021 acaaaaaata tcctcttcat cgtgggtggt gcttttgatg gtattgaaga aattgtcaaa
    10081 caacgtctgg gtgaaaaagt catcggattt ggtcaaaaca ataaggcgat tgacgaaaac
    10141 agctcataca tgcaagaaat catcgctgaa gacattcaaa aatttggtat tatccctgag
    10201 ttgattggac gcttgcctgt ttttgcggct cttgagcaat tgaccgttga tgacttggtt
    10261 cgcatcttga aagagccaag aaatgccttg gtgaaacaat accaaacctt gctttcttat
    10321 gatgatgttg agttggaatt tgacgacgaa gcccttcaag agattgctaa taaagcaatc
    10381 gaacggaaga caggggcgcg tggacttcgc tccattatcg aagaaaccat gctagatgtt
    10441 atgtttgagg tgccgagtca ggaaaatgtg aaattggttc gcatcactaa agaaactgtt
    10501 gatggaacgg ataaaccgat cctagaaaca gcctagaggt gactatggaa cttaatacac
    10561 acaatgctga aatcttgctc agtgcagcta ataaatccca ctatccgcag gatgaactgc
    10621 cagagattgc cctagcaggg cgttcaaatg ttggtaaatc cagctttatc aacactatgt
    10681 tgaaccgtaa gaatctcgcc cgtacatcag gaaaacctgg taaaacccag ctcctgaact
    10741 tttttaacat tgatgacaag atgcgctttg tggatgtgcc tggttatggc tatgctcgtg
    10801 tttctaaaaa ggaacgtgaa aagtgggggt gcatgattga ggagtactta acgactcggg
    10861 aaaatctccg tgcggttgtc agtctagttg accttcgtca tgacccgtca gcagatgatg
    10921 tgcagatgta cgaatttctc aagtattatg agattccagt catcattgtg gcgaccaagg
    10981 cggacaagat tcctcgtggt aaatggaaca agcatgaatc agcaatcaaa aagaaattaa
    11041 actttgaccc aagtgacgat ttcatcctct tttcatctgt cagcaaggca gggatggatg
    11101 aggcttggga tgcaatctta gaaaaattgt gaggaaaaga aaatggcaaa aacaattcat
    11161 acagataagg ccccaaaggc tatcgggccc tatgttcaag gaaaaatcgt tggcaacctt
    11221 ttgtttgcta gcggtcaagt tcccctatcc cctgaaactg gggaaattgt aggagagaat
    11281 atccaagaac agacagagca agtcttgaaa aacatcggtg ctattttggc agaagcagga
    11341 acagactttg accatgttgt caaaacaact tgtttcttga gcgatatgaa cgactttgtt
    11401 ccttttaatg aggtttacca aacggccttc aaagaggaat tcccagctcg ttcagctgtg
    11461 gaggtagctc gtcttcctcg tgatgtaaaa gtcgaaattg aagtcatcgc agagattgga
    11521 taagctagtt gaagtttggt gttgccaaac ttcttttgat ataaggagaa aaagatgaca
    11581 aagaaacaac ttcacttggt gattgtgaca gggatgagtg gcgcagggaa aactgtagcc
    11641 attcagtcct tcgaggatct aggttatttc accattgata atatgccgcc agctctcttg
    11701 cctaagtttt tgcagctggt tgaaattaag gaagacaatc ctaagttggc cttggtagtg
    11761 gatatgcgta gccgttcttt cttttcagag attcaagctg ttttggatga gttggaaaat
    11821 caagatggtt tggatttcaa aatcctcttt ttggatgcgg ctgataagga attggtcgct
    11881 cgttacaagg aaaccagacg gagtcaccca ctagcagcag acggtcgtat tttagatgga
    11941 atcaagttgg aacgtgaact cttggcacct ttgaaaaata tgagccaaaa tgtggtggat
    12001 acgactgaac tcactccacg tgagctgcgc aaaacccttg cagagcagtt ttcagaccaa
    12061 gaacaagccc agtctttccg tatcgaagtc atgtctttcg gatttaagta tggaatcccg
    12121 attgatgcgg acttggtctt tgatgtccgt ttcttgccaa atccctatta tttaccagaa
    12181 ctgagaaacc aaacgggtgt ggatgaacct gtttatgatt atgtcatgaa ccatcctgag
    12241 tcagaagact tttatcaaca tttattggcc ttgattgagc cgattctgcc aagttaccaa
    12301 aaggaaggta agtccgtttt gaccattgcc atgggatgta cgggtggaca acaccgtagt
    12361 gtggcatttg ctaaatgctt ggcgcaggac ttatccaaga attggtctgt taatgaaggg
    12421 catcgcgaca aagaccgcag aaaggaaacg gtaaaccgtt catgagaaaa ccaaagataa
    12481 cggtgattgg tggagggact ggaattcccg tcattctaaa aagtctgcgg gaaaaagatg
    12541 tggaaatcgc agctatcgtg acggtggcag atgatggtgg ttcttcaggt gaactccgaa
    12601 aaaatatgca acagttgaca ccgccaggtg atcttcgtaa tgtccttgtg gccatgtcgg
    12661 atatgcctaa gttttatgag aaggtctttc agtatcggtt ctctgaggat gccggagcct
    12721 ttgctggcca tccattggga aatctcatca ttgctggctt gtcagaaatg cagggttcaa
    12781 cctataatgc catgcagtta ttgagcaaat ttttccatac aacagggaaa atttatcctt
    12841 ccagtgacca tcctttgacc cttcatgcag tctttcagga tgggacagaa gtggctggag
    12901 agagtcatat tgtagaccat cgaggcataa ttgacaatgt ctatgtgacc aatgccctaa
    12961 acgatgatac gcctctggcc agccgtcgag tagtgcagac catccttgaa agtgacatga
    13021 ttgtcctagg gccaggttcc ctctttacct ctattttgcc caatatcgtg attaaggaaa
    13081 ttgggcgggc tcttttggaa actaaggcag aaattgccta tgtctgcaat atcatgaccc
    13141 aacgtgggga gacggaacac tttacagata gcgaccacgt ggaagtcttg catcgtcacc
    13201 ttggtcgccc ttttatcgac actgtcttgg tgaatattga aaaagtgcct caggaataca
    13261 tgaattccaa ccgttttgat gaatacttag tgcaagtgga acacgatttt gtaggtcttt
    13321 gtaagcaagt ttcgcgcgtg atttcatcta acttccttcg tctggaaaat ggcggtgcct
    13381 tccacgatgg agatttgatt gtggacgagt tgatgcgcat tatacaggtg aaaaaatgag
    13441 tttcacagta gcagtaaaag aagaaatcct gggccaacac cacctgagcc ggcatgaatt
    13501 atctgccatt atcaaaatgt ctggtagcat cggtctctcg acttcgggct tgactttgtc
    13561 agttgtgaca gaaaatgcca aactggcccg tcacctctat gagtcctttc tccatttcta
    13621 tgaaatcaaa tctgaaattc gtcactacca acggagcaat cttcgcaaga atcgcgtcta
    13681 taccgttttt acagatgaaa aggtgcagga tttgttaagt gatttgcact tggcagactc
    13741 tttctttggc ctggaaacag gtattgatga ggcgatttta tcggacgagg aagcaggtcg
    13801 tgcctatctc tgtggcgctt tcttggcaaa tgggagcatt cgtgaccctg agtcaggcaa
    13861 gtaccagttg gaaatcagtt ctgtttatct ggaccacgcg caaggaattg cctcccttct
    13921 ccaacagttt ttactggatg ccaaggtgct tgagcgcaag aagggggctg tgacctatct
    13981 ccagcgagca gaagacatta tggacttctt gatagtaatc ggagccatgc aggcacgtga
    14041 tgattttgag cgggttaaga ttttgcgaga aacccgtaac gacctcaatc gggctaataa
    14101 tgccgagaca gctaatatcg ctcggacagt ttctgccagc atgaagacca ttaacaatat
    14161 cagtaaaatc aaagatatca tgggcttaga aaatttgcca gtagatttgc aggaagtggc
    14221 gcaactgagg attcagcacc cagactactc tatccagcag ttagcagata gtctcagcac
    14281 ccctctgacc aaaagtggtg tcaatcacag actcagaaaa atcaataaga tagcggatga
    14341 attataaaac cacgaatcct atgtgactcg tggttctttt ttataaacta gtagagtgtt
    14401 ttggttgtac tttttgttca gggtcaatgt agttgatagc gttgttgaca gcagttggag
    14461 cttctccgag gcctgtcgca atcagatcaa tttttccgtc atagtagcag cagtcaccga
    14521 tagcatagat acctgcttgg ctggattcct gtttgctgtt gacgataatc ttgtgacggt
    14581 tgaggtcgag cccccagttt ttaaggttac cgacagaaga tttgaaacca tagttgacaa
    14641 agaggtggtc taggtcaatg gtttcagttt catcagattt gacttttgtg atttcaagtt
    14701 tatcaagtgt ttttccattt ccaaggagtt ggctaggggc gaatggtgtc ttgatggtta
    14761 cagatgattc ttgcaaggct tgaacactgt gttccaaggc acggaaatta tctctgcggt
    14821 gaacaagggt agttggtgcg attttttcaa aagccaaagc ccaatccaca gccgagtctc
    14881 ccccaccaag aatcgtcact ttcttaccag cgtattgctg aatgttagaa acgtggtagt
    14941 ggatattttc atagccctca accccttcaa gttccagcgg acgtggtttg aaggcaccgc
    15001 cacccatagc gatgataact gttttagtca ggtgacttcc tttagaagtt gtgatggcaa
    15061 attcttcttg tttgtcaatc tcaagaaccg tttcattgag atgaataggg gtatcaaatc
    15121 catttagctg ttcaatcaag cggttagtca actcttctcc agtcaggttt gggaagcctg
    15181 gtacgtctag gatttccttt tcagggtaga gaatagcagg ttgtccacct agctggggaa
    15241 gagagtcgat gatttgaacc ttggcttggc gtaggtgggc ataaaaggct gcaaaaagcc
    15301 cgacaggacc accacccaca atggtaatat catagagttg agacatagtt tctcctttgt
    15361 tttttctagt cagtttattt tatcatattt ttgttcaatg taaaatggac taggataggg
    15421 aaaaaaatgc cagaaaatgg tataatagga cgaaagtgtt ttgataggga ggatatcagg
    15481 gatgaatttt caacaattat ccaatctgca atattggacc agtttgtttg caagtccatg
    15541 gacgatagct atcaatctga ttgatatttt gattgttgct tatattttat accattttac
    15601 aaaagctatt gcaggaacca agattatgat tttggtacgt ggagttttgg tgtttatttt
    15661 agctcaaatc cttgcaaata tgattggttt gactacgatt tcttggttaa tcaatcaaat
    15721 tattacttat ggggttattg cggcggttgt tatcttctct ccagagattc ggactggttt
    15781 ggaacgtttg ggaagagcga cagatttttt ttccaatgcc cctattagtg ctgaggaaca
    15841 gatgattcgt gcctttgtta agtctgttga atacatgagt cctcgtaaaa tcggggcctt
    15901 ggttgctatt cagcgtgtac gtaccttgca ggagtatatt tcgacaggaa ttcccttgga
    15961 tgctaagatt tctgcagaac ttctcattaa catttttatt cccaacactc ccctacatga
    16021 tggtgcggtg attatcaaag aagaacgtat cgctgtgacg tctgcctatc tgcccttgac
    16081 aaaaaacaca gggatttcca aggaatttgg gaccagacac cgggcggcta tcggtttatc
    16141 agaagtctca gatgccttga cttttgtcgt atcagaggaa acgggaggaa tttcgataac
    16201 ctataatgga aggtttaagc acaacctaac acttgatgaa tttgaaacag aattacgtga
    16261 aatcttactt ccaaaagagg aagtgggtct tagttttaaa gaacgattgc taggaggatg
    16321 gaaacatgaa aaaaaatagt ttatatatca tatcctcact cttttttgct tgtgtcttat
    16381 ttgtctatgc tacggcgacg aattttcaaa acagtaccag tgctaggcag gtaaaaacgg
    16441 aaacctatac taatacagta acaaatgtcc ctattgacat acgctataat agtgataagt
    16501 attttattag cggttttgct tcagaagtat cagtggtctt gactggggca aatcgcctat
    16561 cgctagctag tgaaatgcaa gaaagtacac gtaaattcaa ggttactgct gacctaacag
    16621 atgccggtgt tggaacgatt gaagttcctt tgagcattga agatttaccc aatgggctga
    16681 ccgctgtagc gactccgcaa aaaattacag tcaagattgg taagaaggct cagaaggata
    16741 aggtaaagat tgtaccagag attgacccta gtcaaattga tagtcgggta caaattgaaa
    16801 atgtcatggt gtcagataaa gaagtgtcta ttacgagtga ccaagagaca ttggatagaa
    16861 ttgataagat tatcgctgtc ttgccaacta gcgaacgcat aacaggtaat tacagtggtt
    16921 cagtaccttt gcaggcaatc gaccgcaatg gtgttgtctt accggcagtt atcactccgt
    16981 ttgatacaat aatgaaggtg actacaaaac cagtagcacc aagttcaagc acatcaaatt
    17041 caagtacaag cagttcatcg gagacatctt cgtcaacgaa agcaactagt tcaaaaacga
    17101 attaaaaata gaaaaggatt ttataacaat gggtaaatat tttgggactg atggagtccg
    17161 tggagaagct aacctagaac taacaccaga attagccttt aaactaggac gttttggagg
    17221 ctatgttctt agtcaacatg aaacggaagc gccgaaagtc tttgtaggac gtgacacacg
    17281 tatttcaggg gaaatgctgg aatcggcctt ggtggcaggt ctcctttcag tagggattca
    17341 cgtatacaaa cttggtgtcc ttgcaacacc agcagtagct tacttggttg aaactgaagg
    17401 agcaagtgcc ggtgtcatga tttctgctag ccacaaccca gcccttgata acggaatcaa
    17461 gttctttggc ggtgatggct tcaaactaga tgatgaaaaa gaagcagaaa ttgaagcctt
    17521 gctagatgct gaggaagaca ctcttcctcg tccaagtgca gaaggcttag gaattttggt
    17581 agattatcca gaaggcttgc gtaagtatga aggatacctt gtttcaactg gaactcctct
    17641 tgatggaatg aaggttgcct tggatacagc taatggagca gcttctacca gtgcccgtca
    17701 aatctttgca gaccttggtg cccaattgac ggttatcggg gaaacaccag acggtcttaa
    17761 catcaacctt aatgttggtt caacacatcc agaagccctt caagaagtgg tcaaagaaag
    17821 tgggtcagct attggtttgg cctttgatgg agacagtgac cgcttgattg ctgttgatga
    17881 gaatggtgac atcgtcgatg gtgacaagat tatgtacatc atcggaaaat acctttctga
    17941 aaaaggacaa ttggctcaaa atacaattgt gacaactgtt atgtctaacc ttggtttcca
    18001 caaggccttg aatcgcgaag gtattaacaa ggcagttact gcagttggtg accgctacgt
    18061 tgttgaagaa atgagaaaat caggctacaa ccttggtggt gaacagtctg gtcacgttat
    18121 cttgatggat tacaatacca caggtgatgg tcaattatca gcagttcaat tgactaaaat
    18181 catgaaggaa actggtaaga gcttatcaga gttggcggca gaagtaacga tttatccaca
    18241 aaaattagtt aatatccgag tggaaaacgt catgaaggaa aaggccatgg aagtgccagc
    18301 tatcaaggcc atcatcgaga agatggaaga agaaatggcg gggaacggcc gtatccttgt
    18361 tcgtccaagt ggaacagagc ccctcttgcg tgttatggca gaagcgccta caacagaaga
    18421 agtggactac tatgttgata ccatcacaga tgtagttcgt gctgaaattg ggattgacta
    18481 aatcctagaa aactagatga aaataaaaaa ttatggtaca atagcttata atattgtagc
    18541 cgatggagaa agacatgaat cttaaacgtg aacaagaatt tgttagtcag tatcattttg
    18601 atgcgcgtaa ttttgaatgg gaaaatgaaa atggagctcc tgaaaccaag gtggatgtga
    18661 atttccaatt gctccaacat gaccaagaaa atcaagtgac ttccttgatt gtcatcttga
    18721 gctttatgat tgtatttgat aagtttgtca ttagtggaac aatctcacaa gtaaaccata
    18781 tcgatggccg tattgtcaat gaaccaagtg aattgaacca agaagaggtg gaaaccttgg
    18841 ctcgtccatg tttgaacatg ctcaatcgtt tgacttacga agtgactgaa attgcattag
    18901 acttaccagg aattaatttg gagttctaat atgaaattag ctgttttcac agattcctct
    18961 gcttatctca gtgaagagac cttgcaaaga gaagacttgt ttgtcttgga tattcctgtc
    19021 aatattgatg gtgaggaata tgtcgaaggc atcaatctgt ctgctgagga attttaccaa
    19081 aaaatggctc aggcttctga attgcctaag accagtcaac caagtattgc caagttagat
    19141 gagatcttaa cttcgctcaa agaacaaggc tatacacatg ccttggggct tttcctatct
    19201 tctggaattt caggttttta ccaaaatatc cagtatatgg tcgatgacta tgagggctta
    19261 accattgctt tcccagacac tttgattaca agtgctcccc taggcatcat ggttgaaagc
    19321 gtctttaatt ggcgtgatca gggcgatgat tttgccagca ttcaggataa gctagccatt
    19381 caaatcagcc gtacgtcagc ctttatcatg gtagatgact tggatcattt ggtaaaaggt
    19441 ggacgccttt caaatggggc tgccattttg ggcaatttgc ttagcattaa gccaatcctt
    19501 tattttaacg atcaaggtgt gattgaagtt tacgaaaaag ttcgtactga aaagaaggcc
    19561 accaaacgct taattgaaat tatcaaggaa acaacggctt caggtcaata ccgggtcatt
    19621 gtcattcatg ggaatgatcc tgaaaaggct gaagaattgc gtcagcattt gcttgatttt
    19681 ggcttgggtt cggatgtttc acttgctaca tttggtagtg tcattggaac gcacctagga
    19741 gcaggaagta ttgctctggg ttatattcca gtgatttagt aggcacttct aggctagtta
    19801 atcttagcaa ttgaacacgg actacctgcc tcttgaaaaa agatgtctgt cttgcctttc
    19861 gtggaaagtc agtgccattc cctatttttc atgggtggct aagatccttt gtattttgat
    19921 aattgaacac gcctggaacc ctgtgtgaaa aagatagttc ttccaaggag tagacacttc
    19981 ttgatcagaa ttcctatttt cactttgtgt tcttacaggc tttgtatctt agacaggagt
    20041 agagatgagt attcgagtaa ttattgccgg ttttaaggga aagatgggtc aagctgcttg
    20101 ccagatggtc ttgcctgatt cagatttgga cttggtagca gttttggatc cttttgagtc
    20161 tgagtcagaa tggcagggta ttcctgtttt caaggataag gctgatttag ctggttttga
    20221 agcggatgtc tgggtagact ttactactcc agctgttgcc tacgaaaata cacgttttgc
    20281 tcttgaaaat ggctttgctc cagtagttgg aacgactggt ttcacgagtg aagaaattgc
    20341 agagttaaaa gaattttctc gtgcccaaga cttgggtggc ctgattgccc ctaactttgc
    20401 cttgggtgct gtcttactca tgcaatttgc gacgcaggct gccaaatatt tcccaaatgt
    20461 ggagattatt gagctccatc atgacaagaa aaaggatgct ccgagtggaa cagccattaa
    20521 aacagctgag ttgatggcag aggttcgaga gtccattcag caaggcgcag cagatgagga
    20581 agagctgatt gctggtgctc gtggtgctga ctttgatggt atgcgcatcc actcagttcg
    20641 tttgccaggt ttggtagccc atcaggaagt catctttggc aatcaaggag aaggattgac
    20701 cctccgtcat gactcctatg atcgcatctc cttcatgaca ggagtcaatt tgggaattaa
    20761 agaagttgtc aagcgtcatg agcttgtcta tggattagaa cacttattat gagattaacg
    20821 caaatgcctt ctgaatttca gaaggcttta ccagtattag aaaaaattaa agaagcaggc
    20881 tttgaggctt attttgttgg gggctctgtt cgagatgccc ttctccatag ccctatccac
    20941 gatgtggata ttgcgacgtc ttcttatcca gaagagacca agcagatttt tccgcgaaca
    21001 gccgatatcg gaatcgagca tggaaccgtc ttggtcttag atggggatga ggagtatgag
    21061 gtaacaacct ttcggacaga ggatgtctat gtggactatc gcagacccag tgcggtttcc
    21121 tttgtgcgct cgctagaaga agacctcaaa cgccgtgatt tcacagtcaa cgcctttgcc
    21181 ttggatgaga caggagaaat cgttgacttg ttccatggtt tagaagattt ggaaaagcaa
    21241 gtcttgcgag cagttggagt ggctagtgag cgtttcaacg aagatgcttt acggattatg
    21301 cgtggtttcc gttttcaggc tagtcttggt tttgcacttg agccagaaac atttaaagct
    21361 atgaagacct tgacgccgct tttggagaac atttctgtag agcgtacctt cgtcgagttt
    21421 gataaactct tgctggctcc attttggaga aggggcttgg cttccatgat tgagagtcaa
    21481 gcttatgact atctccctga tatggcatct agccagaaca agctcaacag actgtttgat
    21541 ttggagactg attttacttt tgaatcctct gaacaagcct gggcggcttt actatgggct
    21601 ttggagattg aaaatgcgca gtcatttttg aaatcttgga agacctcacg ccagtttgcc
    21661 aagcaagttc aggatttgct gattattttg gctctgcgtg aaaatggaga attgagcaag
    21721 cgagattgtt atcgctttga catagatttg cttttacagg ctgaaaatct tcgtcaagct
    21781 cgaggaaaag aagtcaaccc acaagccatc acagaaaaat accaaagctt gactatccat
    21841 gacaagaaag agattcagat taatggcgat attttgatca aggaatatgg ctatcagcca
    21901 ggcccagact tgggagagat tttaacagag attgagtttg ccattgtcga tggagaattg
    21961 gagaataatc gtgaagccat ccatgcttac ctgagggaga aaaaatgagt gattttatcg
    22021 ttgaaaaact aagtaaatcc gttggtgaca agaccgtttt tagggatatt tcctttatta
    22081 tccatgactt agacagaatt ggtttaatcg gtgtcaatgg gactggcaag accacccttt
    22141 tggacgtcct ttctggtgtt tctggctttg atggggatgt cagtcctttt tcagctaaaa
    22201 atgattacca gattggttac ttgactcagg atcctgattt tgatgataga aagacagttt
    22261 tggatacggt tctatctagt gaactcaagg aaatccagct cattcgtgag tatgaattga
    22321 ttatgctcga ctatagtgag gacaagcagg cgcgtttgga acgtgtcatg gcagagatgg
    22381 actctctcca agcttgggaa atcgagagtc aggtcaagac cgttcttagc aaattgggca
    22441 ttcaagactt atctactcct gttggggaat tgtcaggtgg tctgagaaga cgggtacagt
    22501 tggcacaagt cttacttggc aaccacgacc tcttgctttt ggatgagccg accaaccatc
    22561 tggatattgc gattattgag tggctgaccc tctttttgaa aaattctaag aagaccgtcc
    22621 tttttatcac tcacgatcgt tatttcttag acgctttgtc aacacggatt ttcgagttgg
    22681 agcgagcagg cttgacagaa tatcagggca attaccagga ctatgttcgc cttaaggcag
    22741 aacaggacga gcgcgatgca actctacttc ataaaaaaga acaactctac aaacaagaac
    22801 tggcctggat gcgcagacaa ccgcaggcac gtgcgaccaa gcaacaagct cgtatcaatc
    22861 gtttccatga tctgaaaaag gaagtttcag gcagtagtgc tgagacagac ttgactatga
    22921 actttgaaac cagtcggatt gggaagaaag tcatcgagtt tcaggatgtt tcctttgcct
    22981 atgaaaataa gcccattttg caagatttta atctcttggt acaggtcaaa gatcgtatcg
    23041 gaatcgttgg ggacaatggt gttggaaaat caaccctact taacctgatt gcaggaagtc
    23101 ttgagccgat agcaggacaa gttgtgattg gggaaactgt tcgcatcgcc tatttctctc
    23161 aacaaattga gggtttggat gaaagcaagc gtgtgatcaa ttacctgcag gaagtggcag
    23221 aggaggtcaa gaccagtggt ggttctacga cttccatcgc tgagttgctg gagcaattcc
    23281 tcttcccacg ttcgacgcat gggaccttga ttgagaaatt atctggtgga gagaaaaaac
    23341 gtctttatct cctcaaactg ctcttggaga aaccaaatgt tcttctttta gacgagccaa
    23401 ccaatgactt ggatattgca actttgacag tcttagagaa tttcttgcaa ggttttgcag
    23461 gtcccgtttt aacagtcagt cacgaccgct atttcttgga taaggtagcg accaagattc
    23521 tcgcttttga ggatggcaag attcgtcctt tctttggtca ttacaccgac tatcttgatg
    23581 aaaaagcttt tgaaacagat atggccaatc aagtgcaaaa ggccgaaaag gaaaaagtgg
    23641 tcaaggttcg agaagacaag aaacgcatga cctaccaaga aaagcaggag tgggcaagta
    23701 ttgaaggtga tattgaaacc ttggaaaaac gtatcgctgc tattgaagag gaaatgcagg
    23761 ctaacggctc tgactttggt aagctggcta ctctccaaaa agaattggat gagaaaaatg
    23821 aagcactcct tgaaaaatac gaacgctatg agtatctcag tgaatttgat agttaagact
    23881 attcgaaaat caaagtgcaa actatgtcgg cttcacattg ccgactcaag tacagcctgt
    23941 gaccggcttc ctaatttgca ctttgatttt tattgagtat aaaagaatag aaaaatcccg
    24001 tctcatgaaa ggatttctct attctttttt ggtttcttga tgaaagatat tttgccaggt
    24061 ttcgtggcga cgccagatac tggtatggat gataccatct aactcatagc agatgagttt
    24121 ggttttttga ctgatggaag taatttgaat gtccttgata gcagaattta actctttttt
    24181 ggtcttataa gcctctttat ccatctgctc tccatcttgg cgaatataga caaaatcatc
    24241 agtcaagagt tcttctagtt ggtttccttg gtcaatcaat tgttcacgca taagcaattt
    24301 tttatagaca ttgtctaaaa tttcatcctc agggataggt gctggttcga tatggacatc
    24361 ggtatcaaag acgccaaaac gctcctccag catagactcg acctgatccg cgatttcatg
    24421 gctttcaaaa acagacaagt caggattcat ctctagtgta atatccaggt agatgttgct
    24481 accgtaggtg cgacctcttt gcgatttgac cttgctgatt ttgggaattt ccatgatagc
    24541 cttttggtag tcctcgagca ggcggtcgtc aaagccatct gaaagactaa aggaagactc
    24601 gatgaagata tcataggcag tcttcaagat aaagaaagtg atgatgatag caaccagttt
    24661 atccacaatc ggataattga aactactagc taggatggca atggcggttc caagtgaggt
    24721 aacagcgtca gaaagattgt ccttagcagc tgccttcagc gccttggagt tggatttctt
    24781 actgaggcga gtattgtaga gatagaccac aaacataatc gctgcagaaa tgattcctag
    24841 agttgcacca agaggatcaa tgaccgtttc ttcccgactg agaatctttt gaatggtatc
    24901 tcttagaata tcgaaaccga catagaacat gatgatagaa gtgatcaagc ttgccaaatc
    24961 ttcaatcttc caatgaccaa aacggtggtc acggtctgca ggctggcgcg ccatccgaat
    25021 cccgattaag agggccacat ttccaatgat gtccgatacg ttattaaaac catcggccac
    25081 caaactggat gaatgaagga gatgaccagc tgctaattta gctgcagaca agatcaaata
    25141 ggtcgaaata ctgataatgg ctccacgctc agctaacttg agatttgaga tagattgctt
    25201 cattcaactt cctttctagt cacatagcat tctaccatta tactggtttt caaggaaaaa
    25261 ttcaaataag gcagttttta ctctttgaaa actccccaaa atttggtata atggtactac
    25321 tcaagggagt agctggcaga aacctgtgat agtgtcgtca ttccgaattt tatgctgaaa
    25381 agtatgcttt ccggccctat cttaaacagc gagacttgtt atgattaaca ggtctctttt
    25441 tgtttgtcgt gaaaagatat gacagaaaga gagtctgttt tgcacacaat gtcaaggagg
    25501 agacacatgt caaaagaaca aaaacgccaa gcgttttata ctcagagccc tgaagaggtc
    25561 ttgcaggctg tggatgcgac cgagcaaggt ttgtcatcaa gtgaggcgga aaagcgcctt
    25621 gccgaatttg ggcacaatga actcgaagaa ggcgagaaac gatcaatctt ggtcaaattc
    25681 atcgagcaat ttaaggattt gatgattatc atcctagttg cggcagcaat cttgtcagtc
    25741 gtgacttctg gtggggaaga tatcgcagat gccattatca tcctagctgt ggttatcatc
    25801 aacgctgcct ttggtgttta ccaagaagga aaagctgaag aagctatcga agccctcaaa
    25861 tccatgtcta gtccagttgc ccgcgttctt cgtgatggac acatggcaga gattgactct
    25921 aaagaattgg taccaggcga tatcgttgcc cttgaagcag gtgacgtggt accagcggac
    25981 ctacgtttgc tagaagccaa ctctcttaaa attgaagaag cagccttgac gggtgaatct
    26041 gtaccagtcg aaaaagactt gtcagtcgag cttgcgacag atgctggtat tggtgaccgt
    26101 gtcaacatgg ccttccaaaa ctcaaacgta acctatggtc gtgggatggg tgttgttgtc
    26161 aatacaggta tgtacactga agttggtcat attgctggta tgcttcaaga tgcggatgag
    26221 actgatacac cactcaaaca aaacttgaac aacctttcta aggtcttgac ctatgctatc
    26281 ttggtcattg cccttattac ttttgtagtg ggtgtcttca ttcaagggaa aaatccactt
    26341 ggtgagttgt tgacttctgt tgcccttgcc gttgcagcca ttccagaagg acttcctgct
    26401 atcgttacca tcgttctttc tcttggtact caagttttgg ccaaacgaca ttccatcgtt
    26461 cgtaagttgc cagcagttga aacacttggt tcaactgaaa tcatcgcttc tgataagact
    26521 ggtacgctga ctatgaacaa gatgacagtc gaaaaagtct tctacgatgc ggttctacat
    26581 gactcagctg atgatattga actaggtctt gaaatgccac tacttcgttc agttgtcttg
    26641 gccaatgata cgaaaatcga tgtggaaggt aacttgattg gtgacccaac cgaaacagcc
    26701 tttatccaat atgccttgga caagggctat gatgtcaaag gtttcttaga gaaatatcct
    26761 cgtgtagctg aattgccatt tgactctgac cgtaagctca tgtcaacagt tcacccattg
    26821 ccagatggtc gtttccttgt agcagtcaag ggtgcgccag accaactctt aaaacgttgt
    26881 cttcttcgtg ataaggctgg ggatattgct ccgattgatg agaaggttac aaatctcatt
    26941 catacaaaca attctgaaat ggctcatcaa gccttgcgtg tccttgcagg tgcttataag
    27001 attatcgata gtattccaga aaatctcact tctgaagagc ttgaaaatga tttaattttt
    27061 actggtttga ttgggatgat tgaccctgaa cgtcctgaag ccgctgaggc tgttcgtgtg
    27121 gctaaggaag cgggaatccg tccgattatg attacaggtg accaccaaga cacagcggaa
    27181 gccattgcca aacgtttggg aatcattgac gcaaacgata cagaaggtca cgttttaact
    27241 ggtgctgaac tcaatgaact gtcagatgaa gaatttgaaa aagtcgttgg tcaatactct
    27301 gtttatgccc gtgtgtctcc agaacacaag gttcgtatcg tcaaggcttg gcaaaaacaa
    27361 ggtaaagtcg ttgccatgac aggtgacggt gtcaatgacg cgccagctct gaaaacagcc
    27421 gatatcggta tcggtatggg aatcactggt acagaggttt ctaagggggc ttctgatatg
    27481 attcttgcag atgataactt tgcgactatt atcgtcgcag tggaagaagg acgtaaggtc
    27541 ttctcaaaca ttcaaaagac tattcagtac ctactttctg ctaatactgc tgaagtatta
    27601 accatcttcc tatcaacctt gtttggttgg gatgtcttac agccggttca tcttttgtgg
    27661 atcaacttgg taacggatac cttcccagct atcgctcttg gtgttgaacc tgcggaacct
    27721 ggtgtcatga atcataaacc acgtggacgc aaggcaagct tcttctcagg tggtgttttg
    27781 agttctatca tttatcaagg tgtactccaa gcagctcttg ttatgagtgt ttatggcctt
    27841 gcgattgctt acccagttca tgtgggtgac aatcatgcta ttcatgcaga tgccctaacg
    27901 atggcctttg caacccttgg tttgattcag ctcttccatg cctacaatgt caagtctgtc
    27961 taccaatcca tcttgacagt tggcccattc aagtctaaga cctttaactg gtccatcttg
    28021 gtatccttta tccttctcat ggcaacaatc gttgtagaac cgcttgaagg aatcttccac
    28081 gtaaccaaac tagacttgtc acaatgggga attgttatgg ctggaagctt ctcaatgatt
    28141 atcatcgtcg aaatcgttaa gtttattcaa cgcaaactcg gttttgacaa gaatgcgatt
    28201 taagggaggt ttcggatgtc agcatatcaa ttaccgaccg tatggcagga tgaagctagt
    28261 aatcaaggag cttttacggg gctaaacaga ccaacagcag gtgcccgttt cgaacaaaac
    28321 ttgccaaaag gagaacaagc ttttcagctt tattcactgg gaacaccaaa tggtgtgaag
    28381 gttactatct tattggaaga attactagaa gctggtttta aggaagcggc ttacgacttg
    28441 tataagattg ctatcatgga tggggatcaa ttcggctcag actttgtgaa gctcaatcca
    28501 aattccaaga ttccagcctt attggaccag tcaggtactg aaaacgtaag agtctttgag
    28561 tctgctcata ttcttcttta ccttgctgag aaatttggag cctttttacc aagtaatcct
    28621 gtggaaaagg tagaagtttt gaattggcta ttctggcaag caggtgcagc accttttcta
    28681 ggtgggggat ttggacattt cttcaattat gctcctgaaa aattggaata tcctattaac
    28741 cgttttacga tggaagtgaa acgccagttg gatttattgg ataaggaatt ggctcagaaa
    28801 ccttatattg caggcaatga ctatacgatt gcagatattg ctatctggtc ttggtatgga
    28861 cagttagttc aaggaaatct ttaccaaggt tctgcaaaat tcttggatgc ctcaagttat
    28921 caaaatctag taaaatgggc agaaaaaatt gccaatcgtc cagctgttaa gcgtggcttg
    28981 gaagtaactt atacagaaat taaatagcaa aaaagtcatc ttcggatggc tttttgctac
    29041 agttgaggga aagggtttgc agttatcggc ttttgtgcta taattgctat aatatagtaa
    29101 aaagcaagag gagtgtgagg aagtggaaaa gaaaattgtg aaggatatct tatttttatc
    29161 tcaagtgtct cagccggcaa gtcaggagga cctttatctt gccagagatt tgcaggatac
    29221 actcttagca aatcgtgata cctgtgttgg tctagctgcc aatatgattg gggtgcagaa
    29281 gcgcgtgatt atctttaatc ttggcttggt tcccgtggtc atgtttaacc cagtgcttct
    29341 gtcctttgaa ggatcctatg aggcagaaga aggctgtttg tccttggtag gtgtgagatc
    29401 aactaagcgt tatgaaacca caaggcttgc ctatcgtgac agcaagtggc aggaacagac
    29461 cattaccttg acaggcttcc cagctcagat ttgccagcat gagctggatc acttggaagg
    29521 acgaatcatt taggaggaaa gcaaatgaaa cgaatagtct ttgaacttat ttttatcgca
    29581 acgacctggt atatcttttt accgcccctt aacctgacca gctgggaatt tctcttcttc
    29641 ctctgtgggc atttgttagt tgtggcaatc ttatttggct ttggcaaggg gataaacctt
    29701 gtcaaaacgg ttcatgtgcg ccacggtaag gcggaagctg ccttaaatct tgagggtttc
    29761 aaaatcaatc ggttagggaa aattctgtta gcttcgattg gaggaattct tctcttggca
    29821 gctttggttt ccttggtaac ttccagcatg tttcaggcta aaaattatgc caatgtagtc
    29881 acggttacgg aaaaagactt tactgaattt cctaggagtg acaccagtaa ggttcctatc
    29941 ctagatagaa gtactgctga aaaaattgga gaccgctact tgggttccct aaccgataag
    30001 gtgtcgcaat acgtagcggc agatacctat acccaattga caattgatgg gaaaccttat
    30061 cgggtcacac cactagaata tgcagaccct atcaaatggt ttaacaatca agccaaggga
    30121 atcggtgagt atatcaaggt ggacatggta actggaaatg cggatttggt ggacttgaag
    30181 acaccaatca agtattcaga ctcggagtat tttaaccgtg atgtcaaacg tcacctgcgc
    30241 ttgaagtacc cgactaaaat ctttaaaact ccatcttttg aggtggacga tgagggcaat
    30301 cctttctatg tagcaacggt ttaccaaaag caatttggac ttgcggttcc tcgtcctgct
    30361 tcagtcatta tcttggatgc tacaaatgga gaaaccaagg aatacagctt atcagatgtt
    30421 ccagaatggg tggacaggat ctatccagca gaggaaacca ttgagcaaat caactacaac
    30481 ggcaagtaca aggacggttt cttgaatgcc atgatttcca agaaaaacgt gacccagact
    30541 accaatggct ataattactt gtctatcggt aatgacatct atctctacac aggtgtgacg
    30601 tcggctaatg cggatgagag taatcttggt ttcatccttg aaaatatgcg aacaggagaa
    30661 attactaagt atagcttggc ttctgcgaca gaagaatcag cccgtgaatc agcagaaggt
    30721 gctgttcagg agaaatccta caaagcaacc ttcccaatcc tcatcaacct caatgacaag
    30781 cctctctaca tcatgggctt aaaggacaat gctggcttgg tcaaagagta cgccctggta
    30841 gacgcagtcg agtaccaaaa tgttatcgtt gctactacag tggaagagat gctcagcaag
    30901 tatgccaata aaaacgacct tgaaattgac aatgcaacga cagaaagcat caatggagta
    30961 gtagcagacc tcaaatcagc tgttatcaag ggagacactg tctacttctt taaagttgat
    31021 ggcaagatat acaaggtcaa ggcttcagta tccgatgacc ttccttacct tgaaaatggt
    31081 aaaaccttcg aaggtcaagt aggaaaagac aattatctca agacctttaa gctacggtaa
    31141 aaataggttt ttttcagaaa gtatatgtta taataaggta aattaagcct aatggaggac
    31201 aaagaaatgg gtttttattt gatggttgct tcaatgctac tcggactgct cgctttgaag
    31261 ataggttttt ctcagttcaa ggagaaaaaa gataaattct tatctatctt aacaagttta
    31321 gctggcacag cccttgttct cgtggctgtc tggttaggat ggcccaaata aatagaaaaa
    31381 cctcggtcaa accgaggttt ttcaaatgaa tttcttggtt gtggctaaaa aatatgctac
    31441 actatcaata tgaaaatttt aatcccaaca gcaaaagaaa tgaacacaga cttcccaagt
    31501 atcgaggcaa ttcctttaaa accagaaagt caggccgtgc ttgatgcctt ggctctctat
    31561 tctgccagtc aattggagag tttctacaag gtatcagctg agaaagcggc ggaagaattt
    31621 caaaatatcc aagctttgaa aaggcaaact gctcaacact atccagcctt gaaacttttt
    31681 gatgggctta tgtaccgcaa cattaagaga gataagctga ccgaggcgga acaagattat
    31741 cttgaaaatc atgttttcat tacctcggct ttgtacggtg ttgttccagt cttgtcaccc
    31801 atggctcctc accgtttgga ttttttgatg aaattaaaag tcgctggtaa gactttgaag
    31861 agccattgga aggcagccta tgatgaaact ctgaagaagg aagaagtgat tttctctctc
    31921 ttgtcatcag agtttgagac tgtattttct aaggaaatca gagcaaagat ggtgaccttc
    31981 aaattcatgg aggatagagg cggtcagctg aagattcact caactatctc caagaaagcg
    32041 cgcggggcct ttctaacagc tttaatagaa aatcaagtac aaactgtggg ggaagcacgt
    32101 cgcttgaact ttgctggatt tgtttaccga gaagatttgt cacaaccaca gggattggtt
    32161 tttgtaaagg aagtatagaa atcagatatt ttgtatttgg caatatgtat catcagctat
    32221 tttagtttat aaatgagaaa aaagcgaaca agctagtctt ctgagtgtca gaaaagtagt
    32281 cttgttcgct tttattatgt tatactcttt gaaaatctct tcaagccacg tcagtgtcgc
    32341 cttaccgtat atatgttact gacttcgtca gttctatcta caacctcaaa gcagtgcttt
    32401 gagtagcttg cggctagctt cctagtttgc tttttgattt ttattgagtg ttagttactt
    32461 gatagcttcg accaagtctt gagcttgttt ttcaagtgag tttaggactg tttcttcaag
    32521 aaccaatttt ccgtctgccc aggcagagtc attaacacgt gcagcagtga aatcaccaac
    32581 gccttgtgta cggataaatg gcaagaggtc tttgtagata gcgaaaagtt gatcgtgccc
    32641 tgcattggct acagatgata ctgtgacaaa cttgtcttga agggcagaaa cgccacgtgt
    32701 atcagacaag tcaagggcac gagatagcca gtcaagcaag tttttcactg taccagggat
    32761 agagaagttg tagactggag agaaaatcca gatagcatcc gcaacgagaa ctgcttcacg
    32821 agcagcagct acagctggat gtgttggaac ttccaaatct tggctgaaga gaggaagggc
    32881 tgaataatca aggtagctaa cttccgcttt accagcaagt gctttctcag cttcgagcgc
    32941 catttggtgg ttgaaagaac cttgacgtag tgatccgacg ataaataata cttttttaga
    33001 catgatgtgt ctcctttttc ttcaaatttt gagaactctc tcgttgttat aaaatacatt
    33061 acaacaaaac aaaagaactt gcaagaaatt tgctcagagt ttatttgaaa attgtaaaat
    33121 ttcggaaact ctagtcattt gtgactgaaa aagtaagaac tgtttcactt tcaaaatcgg
    33181 ttttgtttga agggagaaat agaattggtt gctcaaagca cagctttgag gttgcagata
    33241 aagctgacgt ggtttgaaga gattttcgaa gagtattctt atattcactt gtaataaagc
    33301 ttaatagatg gtaaaataga cgagtgaaaa aaagacaaaa caaagcaaaa aataatctac
    33361 tgtggcagta tggtctaggg atgacgattt tgtttgtggt tatcagttct tcctttctgt
    33421 atatggtttc tcttagcatg aaaccctatc aaacagctaa aagtgaagga gaaaaattag
    33481 ctcagcagta tgcaggatta gagcaggccg atcaggttga tttatacaat ggcttggaat
    33541 cttattacag cgttcttggt cgtaataaac agcaagaagc acttgctgtt ctgattggaa
    33601 aagatgatca taagatttac gtttatcagc taaatcaggg tgtttcacaa gaaaaagcag
    33661 aaacggtttc taaggaaaag ggagctggcg aaattgacaa gattatcttt ggtcgttatc
    33721 aagataagcc aatctgggaa gtcaagtcag gatctgattt ttatctagta gattttgaaa
    33781 caggagcatt ggtcaacaag gagggcctat gaaattatcc aaccgtgttt tagaaatgga
    33841 agaaagtgtg actttggctg ctggagcccg tgccaaagca ctgaaggctg agggtagaga
    33901 tattttgtct ctaaccttgg gtgagccaga tttcactact cccaaaaata tccaagatgc
    33961 cgccattgca tcgattcgag atgggcgtgc ttctttttat acagtaacct caggtctgcc
    34021 agagcttaag gcggcggtca atagctactt tgagcgcttt tacggctatt ctgtagcgtc
    34081 aaatcaagtg acagtcgctg cgggagccaa atattctctc tataccttct ttatggctgt
    34141 ggtcaatcca ggtgatgaag tcatcatccc aaccccatac tgggttagct atggagatca
    34201 ggtcaagatg gcagaaggcg ttcccgtctt tgtttctgct aaggaagaca atcactttaa
    34261 ggtgaccgta gagcagttag aagcagctcg cactgacaag accaaggttt tggtgctgaa
    34321 ttcgccatct aatcccacag gtatgattta cacccgtgag gaactcttgg caattggaaa
    34381 ctgggctgta gaaaatgata ttctcatcct agcagacgat atctatggcc gcttggttta
    34441 taatggtcat gagttcacac cgatttctag cctatcggaa gcgattcgca agcaaacagt
    34501 ggtcatcaat ggtgtgtcta aaacctatgc catgactggt tggcggattg gttatgccgt
    34561 tggagaagca gacattatcg ctgccatgtc caagattgca ggtcaaacaa cttcgaatcc
    34621 gtcagcagta gcccaatatg cagcagttga ggctctatca ggtgagcaag atactgtaga
    34681 aagcatgcgt caggcctttg aggaacgtct taataccatc tatcccctcc ttgcagaggt
    34741 gccaggattt gaagtggtca aaccgcaagg ggccttctat ctcttcccaa atgtcaaaaa
    34801 ggccatggag atgaaaggct acacggatgt gacagacttt acaactgcta tcttagaaga
    34861 agccgaagtg gccttggtaa caggagctgg atttggagca ccagaaaatg tccgcctcag
    34921 ctatgcgaca gacctagaca cgcttaaaga agcagtcgaa cgcttgaaag catttatggg
    34981 tagtgagaat gattgatcat tttgagatta aggtaaagga tttacaaatt tcagaaggat
    35041 tttataggag ttttctcgct cctttggact ataaattggc ttttaagact agttctctaa
    35101 ttagttttct atccccgaac agccctcatc ctggtggtga tttttggctg actcagggaa
    35161 cacaagatcc tgttcatttt gcttttttag cagaaaataa ggaagaagtt taggcttgtt
    35221 atgaggctgg cttagaggca ggtgggcgag ataatggggc tcctggttat cgaagtgagc
    35281 gtccgattta ctatgctgct tttatgattg acctggatgg gaacaatata gaggtggttt
    35341 gccataaaga ataaaaaaga aaaggaaaaa taatgacaaa acgtgtaacg attattgacg
    35401 taaaagacta tgttggtcag gaagtgacga ttggcgcttg ggttgccaat aaatcaggaa
    35461 aagggaaaat cgctttctta caattgcgtg atggaacagc cttctttcaa ggtgtggctt
    35521 ttaaaccaaa ctttgtcgaa aaatttggtg aagaagtggg acttgagaag tttgatgtta
    35581 tcaaacgctt gagccaagaa acgtctgttt atgtgacagg tattgtcaaa gaggacgaac
    35641 gttctaaatt tggctatgag ttggacatca cagacatcga agtgatcggt gaatctcaag
    35701 actacccaat cacaccaaaa gaacacggaa cagacttttt gatggataac cgtcacttgt
    35761 ggctacgctc tcgtaagcaa gtagctgtgt tgcaaatccg taacgctatt atctatgcaa
    35821 cttatgagtt ctttgacaag aacggtttta tgaagtttga cagcccaatt ctttcaggaa
    35881 atgcggcaga agattctaca gaactctttg aaactgacta cttcggaacg ccagcctact
    35941 tgagccaatc aggtcagctt tacctagaag caggggctat ggctcttggt cgtgtctttg
    36001 actttggtcc agttttccgt gctgaaaaat caaaaacacg ccgtcacttg actgagttct
    36061 ggatgatgga tgctgagtac tcatacttga cacatgatga gtcgcttgac ttgcaagaag
    36121 cttatgtgaa agctcttcta caaggtgttc ttgaccgcgc gcctcaagcc ttggaaacct
    36181 tggaacgtga tacagaactc ttgaaacgct acattgcaga gccattcaaa cgtatcactt
    36241 acgatcaagc cattgacctc ttgcaagagc atgaaaatga tgaagatgct gactacgagc
    36301 atcttgagca tggtgatgac tttgggtcac cacacgaaac ttggatttca aaccactttg
    36361 gtgtgccaac atttgtcatg aactatccag tagccatcaa ggccttctac atgaaaccag
    36421 ttcctggaaa tccagagcgc gtgctttgtg cagacttgct tgctccagaa ggctatggag
    36481 aaattatcgg tgggtctatg cgtgaggaag attacgatgc ccttgtcgct aagatggatg
    36541 aacttggcat ggatcgtaca gaatatgaat tctaccttga ccttcgtaaa tacggtacag
    36601 ttccacacgg aggatttggt atcggtatcg aacgtatggt aacctttgca gcaggaacaa
    36661 aacatatccg tgaagctatt ccattcccac gtatgttgca ccgtatcaaa ccataagaac
    36721 aggaaaacgc ccatgtggcg ttttttcgaa ttctagtgtt tttacttgcg taaaaaaatt
    36781 ttttctagta taatagttta ttgtgagcga acctcactta ccccttgcaa agtcttgggg
    36841 tcattagacc aaaaggagga acatatcaat ggctaaatac gaaattcttt atatcattcg
    36901 tccaaacatt gaagaagaag ctaaaaacgc tttggtagca cgttttgact ctattttgac
    36961 tgacaacggt gcaactgttg ttgaatcaaa aacttgggaa aaacgtcgtc ttgcatacga
    37021 aatccaagat ttccgtgaag gactttacca catcgttaac gttgaagcaa atgacgatgc
    37081 agctcttaaa gagtttgacc gtctttcaaa aatcaacgct gacattcttc gtcacatgat
    37141 cgtcaaaatt gacgcgtaag aaggtaaact atgattaaca atgttgtact tgtagggcgt
    37201 atgacacgtg acgctgagtt gcgttatacc ccatcaaatg tagcagttgc gacttttact
    37261 cttgcagtaa accgtacatt taagagtcaa aatggtgaac gtgaggctga ttttatcaat
    37321 gtcgttatgt ggcgccaaca ggctgaaaat cttgctaact gggctaaaaa aggctcactt
    37381 atcggggtga caggtcgtat ccagactcgt agttacgata accagcaagg acaacgtgtc
    37441 tacgtgacag aggtcgtggc tgagaatttc caaatgttgg aaagccgtag tgtgcgtgag
    37501 ggtcacacag gtggagctta ctctgcacca actgcaaact attcagcacc tacaaattca
    37561 gtactagact tttcacgtaa tgaaaatcca tttggagcaa caaacccatt ggatatttca
    37621 gatgatgatt taccattcta atggacaact aaacttataa aggagaaaaa acatggctca
    37681 acaacgtcgt ggcggattca aacgccgtaa aaaagttgat tacatcgcag caaacaaaat
    37741 tgaatatgtt gattacaaag atactgagct tcttagccgt ttcgtttcag aacgtgggaa
    37801 aatccttcct cgtcgtgtaa caggaacttc agctaaaaac caacgtaaag taacaacagc
    37861 tatcaaacgc gctcgcgtaa tggctttgat gcctttcgta aacgaagatt aaagaaaaga
    37921 cccggttggg tctctttttc atgagcttga aaacaagaaa gcgaattaag accccaatgg
    37981 gtcttttttc acaaactttg aaatgagaga gcgagttagg ctcagtggct ccctttttac
    38041 gagtttgaaa acaagagaac acattaaaag cacgatggag ctttttcata aatatgtctt
    38101 ttttctcatc caagaaatta aaataaaaat gataaacttt ttgaaaatag gggatttgcc
    38161 tactttatga tataatggaa agagttagat tgaaagaggt agtgagatgg acgcgaaatt
    38221 aagatacaag gcaaagaaga tcaagattgt cttttttgat attgatgata cattgcggaa
    38281 ttcaaagaca gggtttattc caactacaat tcccactgtt tttaaacagt tgcgtgaaaa
    38341 aggaatttta acaggaatcg cctctggacg tggcattttt ggtgttgttc cagagattcg
    38401 tgatctcaag cctgactttt ttgtaacttt gaatggggct tatatcgaag ataaaaaagg
    38461 tcaggtcatt tatcagcatc agattgaaaa gtcagatgtt gaggagtata tctcttgggc
    38521 taagcaagaa ggaattgagt atggcttggt tgggagtcat gatgccaagt tgtcgactcg
    38581 caccgatatg atgagtgaag ctatcaatcc aatttatccc gacttagatg tagatcccga
    38641 tttccatgaa aaagaagata tctatcagat gtggactttt gaagataagg gagatgactt
    38701 gcacttgcct gacagtctct cagacaaact tcgcatggtt cgttggcatc aacattcgtc
    38761 tgatattgtg ccgatttcag gctccaaagc gacgggggtg gaaaaagttg tggaacacct
    38821 tggcttgaaa ccagagaagg tcatggtttt tggagatggt ctcaacgact tggaactctt
    38881 tgattatgct ggaatcagcg ttgccatggg aatttctcat gataaaatca aagaaaaagc
    38941 agattatatt acaaaaacat tagaagaaga tggcattttt gatgccttag aagtatttgg
    39001 tatggtagaa aaagaattgc attttccaca agtagacatt gaaacagtag aaggtcctct
    39061 tgcgactatt aagaccaatc acggagactt acgtatcaag cttttccctg aacatgctcc
    39121 taaaacagtg gctaactttg tatctctttc aaaagatggc tactatgatg gtgtcatttt
    39181 ccaccgtatt atcaaggact ttatgatcca aggtggaaac ccaactggaa ctggtatggg
    39241 tggcgagtca atctacggcg aatcttttga ggatgaattc tcagaagagc tttacaatat
    39301 ccgtggtgct ctttccatgg caaatgctgg tccaaatacc aacggcagcc agttctttat
    39361 cgtgcaaaac caacacctac cttattctaa gaaagaaatt actcgtggtg gttggccaga
    39421 accgattgca gaaatctatg ccaatcaagg tgggacacct cacctagacc gtcgtcacac
    39481 ggtttttggt cagttagctg atgaagcatc ttacgctgtc ttggatgcca ttgctgctgt
    39541 tgagacagga gctatggaca agccagttga agatgttgta attgaaacta ttgaaatcga
    39601 ggactaagat gaaaatcggt gataagctaa aggggcgtat tacagggatt cagccctacg
    39661 gtgcctttgt tgagttagag acgggtgata cggggctgat tcacatttca gaaattcgga
    39721 caggatttat tgaaaatatt catgaaacct tgaaagtcga tgaagaggtt caagttcagg
    39781 tagtggattt agatgaattt acggggaaag ccagtctttc tatccgcact ttggaggaag
    39841 aaaagtacca gtttcctaga cggagacgct tttcaagcga ccgctttaac tacggttttg
    39901 ctccctttcg acgaatgcta ccaatctgga ctggagaagc cctgcaccat ttaaaaaaga
    39961 agaagtagtt tagagtttta tattttttgt aatgttaata taaatttgct ataataggac
    40021 catggaagaa gaacaattat taaaatcagg agagcgcatt aaccagctct tttcgacaga
    40081 tatcaaaatc attcaaaata gagaggtttt tagctattcg gtggatagtg ttctcttatc
    40141 acgatttcca cgttttccta agaaggggtt gattgtggat ttctgtgctg ggaatggagc
    40201 agtggggctt tttgctagca ctcgtactca agcacagata ttgtctgttg agattcagga
    40261 gcgtttggcg gatatggctg aacgctctgt ccgtttgaat ggattagagg agcaaatgca
    40321 ggtcatctgc gatgatttga aaaatatgcc tgctcacatt caggggagta aggtggatat
    40381 gattttgtgt aatccaccct atttcaaggt ggatccttat tctaatctga acgagagtga
    40441 acattatctc ttggctcgac acgaaatcac gactaatttg gaagaaatct gtcatagtgc
    40501 ccagagtatt ctcaagtcta atgggcgttt ggccatggtt catcgtcctg atcgacttct
    40561 ggatattttg gatacgttaa aacgacataa tctagcccct aagcgcctgc agtttgttta
    40621 tccaaaaaga gaaaaggaag ccaatatgct tttgattgag gcgatcaagg atggctcaac
    40681 aagtggcttt aaggtcttac ctcctttgat tgtccacaat gatgatggct cttatacgcc
    40741 cgaaatcgaa gagatttatt atggatcata aggcttatat gtacgttctg gagtgtcgtg
    40801 atggatccta ctatataggc tatacgactg atatgagaag acgcctcgct atccacaata
    40861 gtgggaaggg agccaaatat acacgagcac gcttgccagt caaacttatc tatgctcaag
    40921 gttttgccag taaggaagaa gccatgtcgg ctgaagctct tctcaagcgt aagaagaggc
    40981 cacagaagga agaattttta tctgaaaatc aagatagaaa tttactcagt tattttgaag
    41041 aatcgtgggg agtcctttga ctcctcttac ctttgtcaaa aagcgaaaaa aatgctacaa
    41101 taaagaaaat aaacaaaatg aggtattatc atgtccaaga ttctagtatt tggtcaccaa
    41161 aatccagact cagatgccat cggatcatct gtagcttttg cctaccttgc aaaagaagct
    41221 tacggtttgg atacggaagc tgttgccctt ggaactccaa atgaagaaac agcctttgtc
    41281 ttgaactatt ttggtgtgga agcaccacgt gttatcactt ctgccaaagc agagggggca
    41341 gagcaagtta tcttgactga ccacaatgaa ttccaacaat ctgtatcaga tatcgctgaa
    41401 gtagaagttt acggtgttgt agaccaccac cgtgtggcta actttgaaac tgcaagccca
    41461 ctttacatgc gtttggagcc agttggatca gcgtcttcaa tcgtttaccg tatgttcaaa
    41521 gaacatggtg tagctgtgcc taaagagatt gcaggtttga tgctttcagg tttgatttca
    41581 gatacccttc ttttgaaatc accaacaaca cacccaacag ataaaatcat tgctcctgaa
    41641 ttggctgaat tggctggtgt gaacttggaa gaatatggtt tggcaatgtt gaaagctggt
    41701 accaacttgg ctagcaaatc tgctgaagaa ttgattgaca tcgatgctaa gacttttgaa
    41761 ctcaacggaa ataatgtccg tgttgcccaa gtgaacacag ttgacatcgc tgaagttttg
    41821 gaacgccaag cagaaattga agctgcaatg caagctgcca acgaatcaaa cggctactct
    41881 gactttgtct tgatgattac agatatcgtc aactcaaact cagaaatatt ggctcttggt
    41941 gccaatatgg acaaggtcga agcggctttc aatttcaaac ttgaaaacaa tcatgccttc
    42001 cttgctggtg ccgtttcacg taagaaacaa gtggtacctc aattaactga aagctttaat
    42061 gcgtaagatt ttgggtgtca gctcaaaatc ggaaagtcta gtttgcctta tatcgaaagg
    42121 agtttcggct cctttttttc taggagtgaa gtatgttaga aaatggcgat ttgatttttg
    42181 tgagagatgg gtcagacatg ggacaggcca tccagacttc cacaggtaac tatagccatg
    42241 ttgccattta tttggatggg atgatttatc atgctagtgg acaggctggt gttgtctgtc
    42301 aagaaccggc agacttcttt gagtccaatc atttgtacga cctctatgtt tacccagaaa
    42361 tggatatcca gtcggtgaag gaaagagctt gcagacatct tggagcaccc tacaatgctt
    42421 ctttctatcc agatgcagct ggtttctact gctcccagta tatagcagaa atcctaccta
    42481 tttttgaaac tattcctatg aaatttggag atgaggaaca ggagattagt gatttttgga
    42541 gggagtatta catagaacta ggtctgcctg ttcctctgaa ccaagctggg accaatccta
    42601 gtcagttggc agcatcgcct ctgttacaat gtaaagaaag gaatcttcat gattcagatt
    42661 tttaatccat ctcgtttgac gagacagcca tttttggaga attgatccgc tatctggacc
    42721 agtatgagga tgtgattcta cgggaaatta aggctcaatt tccagatgtt gcagttgata
    42781 aactcatgga agagtatata aaggcaagct tgattctacg tgaaaataag cgctattacc
    42841 ttaattttcc tacgcttgaa tcacttgata gtcttgaact ggatcaagag atttttgtca
    42901 gagaagctag tccggtctat caagccttgt tggagcagag ttttgagacg gaattgcgca
    42961 atcaaatcaa tgcagctatt ttagttgaaa agacggactt tgcgcgcatt aaaatgaccc
    43021 tgtccaatta tttttacaag gtcaaacagc agtatccttt gacagaaaaa cagcaggagc
    43081 tctatgacat tttaggagat gttaatcctg agtatgccct caagtatatg acggcttttt
    43141 tgttgaaatt tctcaaaaaa gaccagctta tgcagaaatg ccgtgatatc tttgtggaca
    43201 gtttggttgt cttaggctat attgtgcaaa atgaagatgg aaagtatgag ttggctatcg
    43261 attttgataa ggagagatta actttctact tagcgtgatt tcttgtttct gagtacattg
    43321 tttgactttc cttagtattc ggtataaact atatgtaacc ggtaacacat atcggaataa
    43381 actaaaggag acaatcatat gtcacttgaa aacaaattgg aacaagcaac aggcgctgtc
    43441 aaagaaggtt ttggtaaagt tactggagac agcaagacag aacttgaagg agctgttgaa
    43501 aaaacagttg ctaaggcaaa agacgttgta gaagacgcaa aaggtgctgt agaaggtttg
    43561 aaaaacgttt ttactaaata ataggaaaaa atcaagggtt tcattttccc ttgatttttt
    43621 ctattcttat aaataatttt ctgcgacggc tgtatctcct gggtaggatt ctttcttgcc
    43681 ctggatgatt tggtaacaat cgactccctt acccgcaata ataactgcat ctaattcgtg
    43741 atttgtgata gccattgccg ccttgatggc ttcttggcga tccgcaatct tttcaacagg
    43801 atgattgatg tagctactaa tttcatctgc aatggccatt gggtcttcat agttagggtc
    43861 atcagcagtc agaaagactt gaatctcagg gtgttgattg aggaggaggc caaagtcctt
    43921 acgacgactt tctcccttgt ttcctgttga tcccagaacc agagcaatct ttccggtttg
    43981 atgagtttcc accacattga tgagtttttt cagactatcc ccattgtggg catagtcgat
    44041 gaagaccttg gctccatttt tctgagtgag gacttccata cgaccaggaa cgcgggttgc
    44101 agcgatgcct tttttgatgt cctcaagact tgctccgaga cggagacaag caagtccagc
    44161 agcaactgca ttttcttggt tgaagttgcc aatgagttgg atatcataat ctccagcgag
    44221 tttacccgta gctgaaaagc taaaggcttt ggaattctcg atttggttat caaattggct
    44281 accatagaaa tcatggtctt gatcttcaac ctgttctttc aagactgaga agtggtccat
    44341 gtcactgtta atgatgactg ctcggctatt ttccatcaag agacgcttgt ggtagaaata
    44401 gtcttcaaag ctagggtgtt caatcgggcc gatatggtct gggctgatat tgaggaaaac
    44461 tcccacatca aaggttagac catagacacg tttgaccaga taggattgac tggagacttc
    44521 catgatgagg tgggtacggt cattttgcac agcctgattc atcatgtcaa agaggtcaat
    44581 actctcaggg gttgtcaacg ctgacttaaa gaaagtctcg ccatcaagag ttgtgttcat
    44641 ggtcgacaac atagcaggtc tatgcccttg agataagatg ttataggcga aataggttgc
    44701 tgttgtctta cccttagtac cagtaaaggc aaggagtttg agtttctctt gtggattacc
    44761 atagaactcc atggcaatca aactcatggc tttctttata tcgttcacaa tgatgacagg
    44821 gatatcgact tcgtagtcct tttcagctac ataccaagct aaaccttgtg ttatagcaga
    44881 aagaaggtat tcttttttaa aggcagcgcc ttttgcaaaa aaaagagtgt cttctgttac
    44941 ttttcggctg tcgtagctga tgctatcaaa aacaactttg ctgtagttgt agtggtaatg
    45001 accttggtca ataatttcgc gaaaaaggcc atctttcttt aaaatatcta atacggtttc
    45061 aatcttaatc atactttcta ttgtaaaccg aaagtcgcaa atttacaagt aacaaggaaa
    45121 agtttataat ggaagataag gagtttttcc tagttatcaa aattgaatga ggaatctatg
    45181 tcgcacgaaa acaatcacca gcaggcccag atgttacggg ggactgcttg gctaacggct
    45241 agtaacttta tcagtcgcct actcggggct gtttacatta tcccttggta catctggatg
    45301 ggggcttatg cagctaaggc aaatggtctc tttaccatgg ggtacaatat ctatgcttgg
    45361 ttcttgttgg tttcaacagc ggggattcca gttgcggtgg ccaagcaagt tgccaagtat
    45421 aataccatgc gagaagaaga gcatagcttt gccctgattc ggagcttctt aggctttatg
    45481 acaggactag gcctggtttt tgctttagtc ttgtatgtct ttgctccttg gctagcagac
    45541 ttgtctggcg tgggcaaaga cttgatccca atcatgcaaa gcttggcttg gggagtcttg
    45601 attttcccgt ctatgagtgt tatccgagga tttttccaag ggatgaataa cctcaaaccc
    45661 tatgccatga gccaaattgc tgagcaggtc attcgtgtta tctggatgct cctagcaacc
    45721 tttatcatta tgaagctcgg ttcaggagat tatctagcag ccgttaccca atcaaccttt
    45781 gctgcctttg tcggtatggt agccagtttt gcagtcttga tttatttcct tgcccaagaa
    45841 ggttcactca aaagagtctt tgaaacagga gataagatta acagtaagcg tctcttggtt
    45901 gataccatta aggaagccat tccttttatc ctgacagggt ctgccatcca gctcttccag
    45961 attttggatc agctgacctt tatcaatagt atgagctggt ttaccaacta cagcaatgag
    46021 gacttggttg tcatgttttc ttatttctca gccaatccta ataaaatcac gatgattttg
    46081 atttctgtag gggtttcgat tgggagtgtt ggtttgccac ttttgacgga aaactatgtc
    46141 aagggggact tgaaagcagc ttctcgtctg gttcaggaca gtctcaccct actctttatg
    46201 ttcttgctac cagcaacggt tggagtggtt atggtaggag aacctcttta tacggtcttc
    46261 tatggtaagc cagatagttt ggctctgggc ttatttgtct ttgcagtttt gcagtctatt
    46321 attttaggct tgtacatggt cttgtctcca atgcttcagg ccatgttccg caaccgcaag
    46381 gccgttctct attttatcta tggttctatt gccaagctag tcttgcaact acctaccatc
    46441 gccctcttcc acagttatgg tcctttgatt tcaacaacca ttgctctcat cattcctaac
    46501 gtcttgatgt atcgggatat ttgtaaagta actggtgtca agcgcaaggt gattttgaag
    46561 cgaaccattt taatcagttt gctgacccta gtcatgtttc tgttaatagg aaccatccag
    46621 tggctgttag gatttttctt ccaaccaagt ggacgtttgt ggagcttctt ttatgtagct
    46681 cttgtcggtg ccatgggggg tggactttat atggttatga gtctgcgtac ctatttatta
    46741 gataaggtaa taggaaaagc ccaagcagat cgcctgcgag caaaatttaa gctttcgtaa
    46801 ggtagcattt ataaataaaa ggaataagtt gaacaatctt ttaggagaac ttgaaactta
    46861 ttcctttttt agcgtaaaaa tagctaaaat tatctttcaa aaatagaaaa tgaggttttt
    46921 ttcgattttt tggaaaaata attgactaaa caaaccaaac gttgtataat aaaacaaata
    46981 tttaaagcag gaggttctgg aaatgaaaaa gtctaagagc aaatatctaa ccttggcagg
    47041 tcttgtcctg ggtacaggag ttttattgag cgcgtgtgga aattctagca cggcgtcaaa
    47101 aacctacaac tatgtttatt caagtgatcc atctagcttg aactatctag cagaaaaccg
    47161 cgcagcaaca tccgatattg ttgcaaattt ggtagacggg ttattagaaa atgaccaata
    47221 tgggaatatt attccatcat tagcagagga ttggactgtt tctcaggacg gtttgaccta
    47281 tacctacaaa cttcgtaagg atgccaagtg gtttacttct gagggagaag aatatgcgcc
    47341 tgtaactgcc caggattttg tgacaggttt gcaatatgca gctgataaaa aatcagaagc
    47401 cttgtatcta gtgcaggact ctgttgctgg tttggatgac tatatcactg gtaaaacaag
    47461 cgacttttca actgtcggtg tcaaggcact tgatgaccaa acggttcaat atactttggt
    47521 taaaccagaa ctttactgga attcaaaaac acttgcaacg atactttttc ctgttaatgc
    47581 agatttcctg aaatcaaaag gggatgattt tgggaaggcg gatccatcta gtattttgta
    47641 caatggacct ttcttgatga aagcacttgt ctcaaaatct gctattgaat ataagaaaaa
    47701 ccctaattac tgggatgcta agaatgtctt tgtagacgat gtgaaattga cctactatga
    47761 tggtagcgat caagaatcac tggaacgtaa ttttacagct ggtgcttata ctacggctcg
    47821 tctttttcct aacagctcca gctatgaagg gattaaagaa aaatacaaaa acaatatcat
    47881 ctatagtatg caaaattcaa cttcatattt ctttaatttt aacctagata ggaagtctta
    47941 caattatact tctaaaacaa gtgacattga aaagaaatcg actcaggaag cagttctcaa
    48001 taaaaacttc cgtcaggcta tcaattttgc ttttgacaga acatcttatg gggctcagtc
    48061 tgaagggaaa gaaggtgcaa caaagatttt gcgtaaccta gtggttcctc caaactttgt
    48121 cagtatcaag ggaaaagact ttggtgaagt tgtagcctct aagatggtca actatggtaa
    48181 ggaatggcaa ggtatcaact ttgcggatgg tcaagaccct tactacaatc ctgagaaagc
    48241 caaggctaag tttgcggaag ctaagaaaga actcgaagca aagggtgttc aattcccaat
    48301 ccacttggat aagactgtgg aagtaacaga taaagtaggc atacaaggag ttagttctat
    48361 caaacaatca attgaatctg ttttaggttc tgataatgta gtgattgaca ttcagcaatt
    48421 aacatcagat gagtttgaca gttcaggcta ctttgctcaa acagctgctc agaaagacta
    48481 tgatttatat catggcggtt ggggacctga ttatcaagac ccgtcaacct atctcgatat
    48541 ttttaatact aatagtggag gagttctaca aaatcttgga ctagagcctg gagaagccaa
    48601 tgacaaggct aaggaagttg ggctggatgt ctatactcaa atgttggaag aagctaataa
    48661 agagcaagat ccagccaaac gttatgagaa atatgctgat attcaagctt ggttgattga
    48721 tagttcttta gttcttccaa gtgtttcgcg tgggggaaca ccatcattga gaagaaccgt
    48781 accatttgct gctgcctatg gtttaaccgg tacaaaaggg gttgaatcat ataaatacct
    48841 caaagtacaa gataagattg tcacaacaga cgaatatgca aaagccagag aaaaatggtt
    48901 gaaagaaaaa gaagaatcca ataaaaaagc ccaagaagaa ttggcaaaac atgtcaaata
    48961 aaatctaatt gtagataagt ttgtgtaaga agtctttgtg cttaaaaacg cataatatca
    49021 ggtatataaa atcctagatg ttatgcgttt tgttatagga agatttatta gattttatcc
    49081 tcaaattgag ttattcccga ctttatccgt tgaaatatta taaggagtat ttatgaaatt
    49141 aaaattatta agagtagata ctaaggtgat tatggggagt ttcttccttg ttctgtctag
    49201 tctacttgct ttgttgcttc cccttatctt aaaaggttta atagatggga gttctattga
    49261 aaatataggt tccaaagtat ttcaatcgtt tttgattttt attggtcaag ccttgttttc
    49321 ttctattggt tactatctgt ttagtcaatc gggtgaaaaa agatagcaaa aatcaggaaa
    49381 aaagtgatag aggggttgat ttatgcagag aaatccttct ttgataagag ccaaagtggg
    49441 gagttgactt ctgccattgt caatgacacg agtgtcattc gtgagttttt aattacgact
    49501 ttcccaaata ttattctgag tttagttatg gtacttggtt ccattgtagt cttatttagt
    49561 cttgattgga atctttctct acttttattc atcactcttc cttgtatgat gtttattatc
    49621 ttgccccttt ccaatatcag tgaaaagtat agtcgtcgtt tacaggagga aatcggattt
    49681 ttaacagggc aattgactga aaagattcaa gaacatgaat taattaagac taaccaagca
    49741 gagaaaagtg tacaggatgt tttggataat tgtattgaaa gggtacaaaa caattcactg
    49801 aaatcagata ggattacttc ttttgagacc ccgtttgctc tcttatttat ctttgcgact
    49861 atagctgtga tgctaaccta tgggggttat cgggtcagcg caggatatat atctgtggga
    49921 accttggttt cgtttttgat ttacctcttt caattactta atcctattag taatatagct
    49981 aattttgtaa ctgtttattc taggagcaag ggatcttcag ttgcactgga gaacttgctt
    50041 gcagttccta aagaaaaatt tgagggagga aaatcggtat caggacgagg gttgaatttt
    50101 aaccatgtct attttggtta tgatgaaaat cgacctgtct taaaggatat tatttgttca
    50161 attttcaagg ggcaaaaaat tgcttttgtt ggaccatctg gatcaggaaa atcaacgatt
    50221 gtgcgtttgt tagagcggtt ttataaaccg ctttcaggag atattctaat ggagcaatca
    50281 agtatatatg attttaactt aaaagaatgg agaagtaaaa tcgcttgggt ttcacaaaat
    50341 aatgcagtct tatctggcag tattcgtgac aatctttgtc tcggtttgaa tcgcttagta
    50401 actgatgatg aattgatgaa agtgctagac ttagtatcac taggtgatga gattcgctcc
    50461 atgaaagagg gactagatac tgaagttggt gaacgcggac gactcttgtc aggggggcaa
    50521 agccaaagac ttcaaatagc tagagcctac ttaaaagatg ctgaaattct tatatttgat
    50581 gaagccactg ctaatcttga tgcggattct gagtatgcga ttatcagtag cctctattct
    50641 gtattaaagg agaagacggt tgtgattata gcgcatcgtt tgtcaacggt aaaagatgtg
    50701 gattgtattt tcttcttaga ggaggggaaa atcactggct caggaactca taaggaacta
    50761 ctggaaaatc atgagcgtta tgctcgtttt gtgcaggagc aaatgataga gtgaaggtgt
    50821 cttttgagat tcaccatttt atagtctatt aaagggagca ggaaaaactc cctttttata
    50881 tagtttgaaa ctataactag ctcttgaaaa caagaaaacg aaggatacaa aataagtggt
    50941 acaatagtta ctatagattt ggaggaattg tatgagcaag gaattacaca ttaacacaat
    51001 tttagcacaa gcagggatca agtctgatga ggcaacaggt gctttggtga caccacttca
    51061 tttttcaacg acctatcagc atccagagtt tggtcgatct actgggtttg actatacgcg
    51121 cactaaaaat ccaactcgta gtaaggctga ggaagtcttg gcggctattg agtcagcaga
    51181 ctatgcccta gcgactagct cagggatgtc agctattgta ctggccttta gcgtctttcc
    51241 agtaggaagt aaggtcttgg cagtgcgtga tctttacggt ggttcttttc gctggtttaa
    51301 ccaagtggag caggaaggtc atttccattt tacctatgcc aatacagaag aagagttgat
    51361 tgccgagtta gaaaaggatg tggatgttct ctatatcgaa accccaacca atcccttgat
    51421 gttggaattt gatatcgaaa aactagcaaa attggctcat gctaagggtt ccaaagtggt
    51481 ggtggacaat accttctata gccctattta ccaacgtccg attgaagatg gagcagatat
    51541 cgttctccat tcagcaacca agtatctagc aggccacaat gatgtcttgg ctggagtggt
    51601 tgtgaccaat agtttagaac tatacgagaa gcttttttac aatcttaata caacaggggc
    51661 agtcttgtct ccatttgaca gctaccagtt gcttcgtggt ctcaagacct tgtctcttcg
    51721 tatggagcgc tcaacagcta acgcccaaga agtggttgcc tttttgaagg attctccagc
    51781 agttaaggaa gttctctaca ctggtcgtgg aggcatgatt tcctttaaag tagccgatga
    51841 aacacgcatt cctcatattt tgaacagtct caaggtcttc tcttttgcgg aaagtttggg
    51901 cggagtggaa agtcttatta cttatccaac gactcaaact catgctgata ttccagcaga
    51961 agtacgccat tcttatggtt tgacagatga cctcttgcgt ttgtctattg ggattgagga
    52021 tgctagagat ttgattgcag atttgcgcca agccttagaa ggataagaca aagatgggaa
    52081 aatatgattt tacaagcctg cccaaccgtt tagggcacca tacctataaa tggaaagaaa
    52141 cagaaacgga tagtgaagtt ctaccagctt ggatagcgga tatggacttt gtggtcttgc
    52201 ctgaaatccg ccaagccgtg caaacttacg cagaccaact ggtttatggt tatacctatg
    52261 ccagtgaaga cttaattaag gaagttcaaa agtgggaagc tacacaatac ggttacaact
    52321 ttgacaaaga ggctcttgtc tttatcgagg gtgtggtacc agccatctca acagctattc
    52381 aaacctttac aaaagaaggc gaggcggttt taattaacac gcctgtctac ccaccctttg
    52441 ctcgcagtgt caagttgaat aatcgtagat tgattactaa ttccttagtg gaaaaggata
    52501 gtctgtttga gattgacttt gaccaacttg aaaaggattt ggtggaagag gaggttaaac
    52561 tctatgttct ttgcaaccct cacaatcctg gtggacgtgt ttgggaaaaa gaagtgttgg
    52621 agaagattgg ccaactctgc caaaaacacg gtgttttgtt agtttcggat gagattcacc
    52681 aagatttgac cctctttggt cacaaacacc agtctttcaa taccatcaat cctgccttca
    52741 aaaattttgc tatcgtcttg agcagtgcca ctaaaacatt taatattgct ggaacaaaaa
    52801 attcctatgc agtcattgaa aatcctaagt tgagactggc tttccagaaa cgcctgttgg
    52861 ccaataatca gcatgaaatt tcaggcttgg gttatttggc gacagaagct gcctatcgat
    52921 acggtaaaga ttggctagag gaactcaagc aagtctttga agaccacatc aattatgtgg
    52981 tggatctatt tggaaaagag actaaaatca aggtcatgaa accgcaaggt acctacttga
    53041 tttggcttga cttttcagcc tatgacctga ctgatgaaac attgcaagag ttgttgagaa
    53101 atgaagccaa ggttatcctc aaccgtggtt tggattttgg agaggaagga agtctccatg
    53161 cccgcatcaa tatagctatg cccaaatctc tgttgcaaga agtctgtcag cggattgtgg
    53221 ctacttttgc caaatgttaa aaatccagcc ttctaggaga aaagtcttcc tagaaggcta
    53281 ttttcatagg cgaaaatatg gtataataaa aagataaggt aaaggtgaaa atatggctaa
    53341 attgattccg gggaaaattc gtatcgaagg tgttgccctt tatgaaactg gtaaggttga
    53401 tatcatcaag gaaaagaaca atcggctcta cgctcgcgtt gcagaagaag aactgcgcta
    53461 tagtttagag gatgatttgg ttttttgtgc ctgtgatttt tttcaaaaga ggggctactg
    53521 tgtgcatttg gcagcgctag agcattttct gaaaaatgat gagcgtggtc aggaaatctt
    53581 gtggagtctg gaagaaggtc atgaagaaaa agaggccgtt gaaaccaagg tgaccttggg
    53641 tggcaagttt ttggatcgaa ttttatctcc aaaatcagaa tgcgcctatg agttatcagc
    53701 tgtgggtcag gtggaagcag gaaccaatca tatcttgtgg actctgagaa tcggccaaat
    53761 caatagccaa aaatactatg tcattcggga tattccactt tttttaagga ttgtcgagca
    53821 gagaaagtcg tatatgattg gcaagattta tgaggagtct ctttcttggg aagcctttga
    53881 tgatgccagc caagaacttt tgactttctt gcacggacta acggaggaag ggctggttcc
    53941 agacctcttt ttccaaaacc aaggtcggca cctctttttc cctctgacct tttttgagca
    54001 gggtgtgaat ttactgatgg ccttgcccca ttttcaattt gaccatcaag tggatagcta
    54061 ccaaaccctg ctttttcagg atatgcatgc tgatgccaat ctatttgcct ttacagtaac
    54121 agaatattcg gactattttg aaatggaaat cagtgagagt cctagggtca atgtctttta
    54181 tcaaggagca gtacttttcc ataaaggtca ggtttatttt ttaacagacc agcagatgcg
    54241 tcttctcaag gaaattaaag cgttacctgt ggatcgacaa ggaaagaaat acctgcaatt
    54301 tgatagcagt gatcgagaca aattggcctc ctgtctgact ctttttatcc agatgggcac
    54361 cgtttcagct ccagaacgtc tacaaatcaa aacttttgcg ccctcttttt actttgacag
    54421 ggaagaagat aatcggattc gtttagaaat tcagtttgat tatggaaata gacaggtatc
    54481 tagccgacaa gagctagaag aattaccatt ttcaagtgac gcggacttag aagaaagaat
    54541 tttccaagtc tgtttggctg cgggctttga agccgatttt caatcttggc gtcaggcctt
    54601 aaaagcggag tctgtctatc atttttttca tgatatcatt ccagtctttg aaaaactcgg
    54661 gcatgttgac ctatcagata aactagaaga gctttatagc ctagcgagtc ctcaggtgca
    54721 gattgcctcc aagggaggtc tcttggaaat ccagtttgat tttcaagata ttgcccagga
    54781 ggaaatcgac caagccatac aggccttggt tgccaatcag gatttttata ttgattcgtc
    54841 taatcaagtt tactttttcg atgaagaaac caagaaaatt cgccaaaatc tacaggaact
    54901 ggggcaattt gaattaaaag atgggacctt gcaggctcgg aaatccttag cctacagttt
    54961 agctcatctc tttgaagggc gagaccgtgt ttctttttca caagaattcc agaatctggc
    55021 ccaggatttg acacatccag aggactttcc tttgcaagca actcaggtca agactgactt
    55081 gcgagattat caggaaaagg gaattggctg gttgcagatg ctccatcatt atggttttgg
    55141 tgggattttg gctgatgata tgggacttgg gaaaaccctt cagaccattg cttttttgac
    55201 tagtcaagtg acaaaagaaa gtcgagtctt gattctcgct ccgtcgggtt tgatttacaa
    55261 ctgggcggat gagtttcaga aatttgctcc acagttggat gtggctgttg ttcatggttt
    55321 gaaagcaagt cgtgaggaga ttcttgccga taaccatcaa atctatgtga ctagctatgc
    55381 caccttccgt caggacagtg agctttatca ggggatggcc tttgacttcc ttttcttaga
    55441 tgaagctcag gtcatgaaaa atgcccagac caagattgcc cagaccttga gacaatttgt
    55501 ggtgccgtcg gtctttgcct tgtcaggaac tccgattgaa aaccatctgg gtgagttgtg
    55561 gtctattttc caaatcgtca tgcccggact tttgccaagc aagaaagaat ttatgaaatt
    55621 gccagcagag cgagtggctc agtttatcaa gcctttcgtg atgcgacgca agaaagaaga
    55681 agttctgact gaattgccag acttgattga ggtgatttat aaaaatgaac tggaagacca
    55741 gcaaaaggct atttaccttg cccagttaca acagatgcga gaccatctgg ctcaagtgtc
    55801 agaacaggaa tttcagcgaa gtcgtgtgga aattttatct ggtttgatgc gcttgcgtca
    55861 aatatgtgac actcctgccc tgtttatgga agattatcag ggagccagcg gcaaactaga
    55921 tagtctccga gacctactgg tacaggtggc agacggcgga caccgtgtct tgattttctc
    55981 tcagttcaag ggaatgttgg aaaaaattga acaagaactg ccagacttgg gcctgacttc
    56041 ctttaaaatt acgggttcaa ccccagccaa ggaaagacaa gacatgacca aggcctttaa
    56101 ccaaggagaa agagatgcct ttctgatttc cctcaaggct ggtggggtcg gtctgaacct
    56161 gacaggtgca gatacagtga tcttggttga cctttggtgg aatcctgcgg tggaagcgca
    56221 agccattggc cgtgcccatc ggatgggtca ggaagaaacg gttgaggtct atcgcttggt
    56281 gaccaaggga accattgaag aaaaaattca ggaactccaa gaacaaaaga aacatctggt
    56341 gtcacaagta ttggacggca cagagtcacg tggtagtctg accctagcag aaattagaga
    56401 aattttggga atttctgaag ccagcacttg aaaaatcaag acaatacgct ataatgaagt
    56461 gttgaaagga aatagaaaat gaaacaagaa cgatttccat tggtgtcaga tgacgaggtc
    56521 atgttgactg aaatgccagt catgaatctc tatgatgagt ctgatctgat cagtaatatc
    56581 aagggtgagt atcgagataa aaattattta gaatgggctc ctattgctga agaaaaacca
    56641 gtaaaaccga ttgaaaagca agtagggaaa cctaaaaaag ctcctttagg ggttaaaaaa
    56701 gaaggaaaga gctatgcgga ggtggcgcgt gaagaagcgc gtgcagactt gaaaaagaaa
    56761 cgctctgcta actatctaac tcaggatttc agccttgcga gacgtcattc tcagcccagt
    56821 ctagttagac agggcaatca accgacaact cctttccaaa aggaaaatcc tggtgaattt
    56881 gtcaaatata gccaaaaatt gacccagtct cattatatct tggcggaaga agttcattct
    56941 atccctacca agaatgaaga agtgtcagca cctgctccaa agaaaaacaa ttatgatttt
    57001 ctaaagaaga gccaaatcta caataaaaaa agtaaacaaa cagaacaaga acgtcgggtt
    57061 gcccaagagt tgaatctgac cagaatgaca gaatagggga gaaaacatgt caaagacata
    57121 tcattttatc ggaattaagg gatcagggat gagtgccttg gccttgatgt tgtaccagat
    57181 ggggcacaag gttcagggat cagatgttga aaagtactac tttacccaac gcggtcttga
    57241 gcaggcagga attaccattc ttccttttga tgaaaagaat ctagacggtg atatggaaat
    57301 tatcgctgga aatgcctttc gtccagataa caacgtcgaa attgcctatg cggaccaaaa
    57361 tggtatcagc tacaaacgtt accatgagtt tctaggtagc tttatgcgtg actttgttag
    57421 catgggagta gcaggagcac atggaaaaac ttcaacgaca ggtatgttgt ctcatgtctt
    57481 gtctcacatt acagatacca gcttcttgat tggagatggg acaggtcgtg gttcggccaa
    57541 tgccaaatat tttgtctttg aatctgacga atatgagcgt cacttcatgc cttaccaccc
    57601 agaatactct attatcacca acattgactt tgaccatcca gattatttca caagtctcga
    57661 ggatgttttc aatgccttta acgactatgc caaacaaatc accaagggtc tttttgtcta
    57721 tggtgaagat gctgaattgc gtaagattac gtctgatgca ccaatttatt attatggttt
    57781 tgaagctgaa ggcaatgact ttgtagctag tgatcttctt cgttcaacaa ctggttcaac
    57841 cttcaccgtt catttccgtg gacaaaactt ggggcaattc cacattccaa cctttggtcg
    57901 tcacaatatc atgaatgcga cagccgttat tggtcttctt tacacagcag gatttgattt
    57961 gaacttggtg cgtgagcact tgaaaacatt ttccggtgtt aaacgtcgtt tcactgagaa
    58021 aattgtcaat gatacagtga ttatcgatga ctttgcccat catccaacag aaattattgc
    58081 gaccttggat gcggctcgtc agaaataccc aagcaaggaa attgtagcag tctttcaacc
    58141 gcataccttt acaagaacca ttgccttgtt ggacgacttt gcccatgctt taaaccaagc
    58201 agatgctgtt tatctagcgc aaatttatgg ctcggctcgt gaagtagatc atggtgacgt
    58261 taaggtagaa gacctagcca acaaaatcaa caaaaaacac caagtgatta ctgttgaaaa
    58321 tgtttctcca ctcctagacc atgacaatgc tgtttacgtc tttatgggag caggagacat
    58381 ccaaacctat gaatactcat ttgagcgtct cttgtctaac ttgacaagca atgttcaata
    58441 ggatgttccc atggaaattc caattaagat cattcaggca agcaagtctg atttgcctga
    58501 gataggggca cttcagacct cgtcttttcc agctgaaaag cagcaacttt cccatatttt
    58561 agaagaaagt atccgtaagt gtgcggatac ctttcttcta gctagggatg aaaatcaact
    58621 tttaggctat attttatcaa gtccccagtc agacaatccg caatatctaa aagtacattc
    58681 tttagtcatc gagtctgacc atcagagaca gggcttggga acacttcttc ttgcagcctt
    58741 gaaagaggtg gcagttgagc tggattacaa agggattcgt ttggagagtc ctgatgagct
    58801 gctttcctat tttgaaatga acggttttgt tgatgaagaa gcaactttgc tctatgcaac
    58861 tagccagggc tatagtatga tttggtttaa tcccttttat ctggaggaac aatgaaaatc
    58921 agaaaagcaa gattagaaga tttggatcgg attgttgaac ttgaatttga aaatttttcg
    58981 gtcgaagaag ccattcctcc ttctgtcttt gaagcacatt tgagagaaat tcagacctct
    59041 tttctggttg ctgaaaaaga aggaagaatc atgggttata tcgaaggacc agttggcctg
    59101 caccgccatc tgcaagacca gtcttttaca gaagaaataa aagactatag tcatgagcct
    59161 ggtggttata tctctgtgac ctgtctgtct attgccaagg aggcacaggg actcggactg
    59221 ggtcagaaat tgctgacagc cttgaaagaa gttgctcttg aagatgagag agatggcatt
    59281 aatctaacct gtcatgacta tctcatcgcc tactatgaaa aacatggatt tgtcaacgaa
    59341 ggccagtccc agtcaacctt tgcaggggaa acatggtatg atatggtctg ggaaatgaaa
    59401 aaataagtta ggaaaagtat catagattgc tttttttgga cttttaagat ataatattat
    59461 ggattatcaa caaggaggaa aaacttttga gtgaaaagtc aagagaagaa gagaaattaa
    59521 gctttaaaga gcagattctg agagatttag aaaaagtaaa aggctatgat gaagttctga
    59581 aagaagatga ggcagtagtt cgcactcctg caaatgaacc ttcaactgaa gaactcatgg
    59641 ctgattcctt gtcaacggta gaggagatta tgagaaaagc tcctaccgtg cctactcacc
    59701 caagtcaagg tgtaccagct tctccagcag atgagattca aagagaaact cctggtgttc
    59761 caagtcatcc aagtcaagat gtaccttctt ctccagcgga agaaagtgga tcaagaccag
    59821 gtccaggtcc tgttagacct aagaaacttg aaagagaata caatgaaacc ccaacaaggg
    59881 tagctgtttc ctatacgacg gcagagaaaa aagcagaaca agcaggtcca gaaacaccta
    59941 cgcctgctac agaaacagtg gatatcatca gagatacatc acgtcgtagc cgtagagaag
    60001 gagcaaaacc cgttaagcct aagaaagaga agaagtcaca tgtgaaagct tttgtgattt
    60061 cattccttgt attccttgcc ttgctctcag caggtggtta ctttggttac cagtacgtgc
    60121 tagattcctt attacctatc gatgctaatt ctaagaaata tgtgacggtt ggaattccag
    60181 aaggttcaaa cgttcaagaa atcggtacga cgcttgaaaa agctggtttg gtaaagcatg
    60241 gtctgatttt tagtttttat gccaagtata aaaattatac cgacttgaaa gcaggttact
    60301 acaatttgca aaagagtatg agtacagaag acttactcaa agagttgcaa aaaggtggaa
    60361 cagatgaacc gcaagaacct gtacttgcga ctttgacaat tccagaaggt tataccttgg
    60421 atcagattgc tcaagctgtg ggtcaattgc aaggtgactt caaagagtct ttgacagcgg
    60481 agactttctt ggctaaagtt caagatgaga cgtttatcag tcaagcagta gcgaaatatc
    60541 ctactttact ggaaagtttg cctgtaaaag acagcggtgc gcgttatcgt ttggaaggat
    60601 accttttccc agctacatac tctatcaagg aaagcacaac tattgagagc ttgattgatg
    60661 agatgttagc tgctatggat aagaacctat ctccttacta tagtactatc aaatctaaaa
    60721 acttgactgt caatgagttg ttgaccattg cttccttggt cgaaaaagaa ggtgccaaga
    60781 cagaagatcg taagctcatt gcaggtgtat tctacaatcg tttgaatcgt gatatgccac
    60841 ttcaaagtaa tattgcaatc ttgtatgccc aaggaaaact ggggcaaaat atcagtctag
    60901 ctgaggatgt tgcgattgat accaacattg attcacctta taatgtttat aaaaatgtag
    60961 gtctcatgcc tggtccagtc gatagtccaa gtctggatgc gattgagtca agcatcaatc
    61021 aaactaagag cgataacctc tactttgtag cagatgtcac agaaggcaag gtctactatg
    61081 ctaacaatca agaagaccac gaccgcaatg tcgctgaaca tgtcaacagc aaattaaact
    61141 aaacaaacta aaattatgtt atacttcaca taattttctt tagaaaatat tatcagaaga
    61201 aagttgagaa aaatggcaga aaaaacatat cctatgaccc ttgaggaaaa ggaaaaactt
    61261 gaaaaagaat tagaagaatt gaaattggtt cgtcgaccag aagtggtaga acgcattaag
    61321 attgcccgtt catacggtga cctttcagaa aacagtgagt acgaagcagc taaggatgaa
    61381 caagcctttg tcgaaggaca aatctctagc ttagaaacaa aaatccgcta tgctgaaatc
    61441 gtcaatagcg acgcagttgc ccaggacgaa gtagcgattg gtaaaacagt caccatccaa
    61501 gaaattggtg aggacgaaga agaagtttat attatcgtag gttcagctgg tgcggatgcc
    61561 tttgcaggta aggtttcaaa tgaaagccca attgggcagg ccttgattgg caagaaaaca
    61621 ggtgatacag caaccattga aacgcctgtt ggtagctatg atgtaaaaat cttgaaggtt
    61681 gaaaaaacag cctaaaaaca gaaaaaggag tggggaggcg atgtgcttca ctcactcctt
    61741 tttccatttt gctattcttc gaaaatctct tcaaaccacg tcagcgtcgc cttgccgtat
    61801 gtatggttac tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagccgact
    61861 tcgtcagttt catctacaac ctcaaaacca tgctttgagc tgacttcgtc agtttcatct
    61921 acaacctcaa aaccatgctt tgagctgact tcgtcagttt catctacaac ctcaaaacca
    61981 tgctttgagc tgacttcgtc agttctatct acaacctcaa aacagtgttt tgagcaacct
    62041 gcggctagtt tcctagtttg ctctttgatt tttattgagt attagatgta tagtgcattg
    62101 aagttaaaat agtacacttt gatttctaag acattgttag aaattagatt aaattcctta
    62161 atcgatttgc gcatgtttta tttcatttca ctatacttta gattggaaaa taaggaatca
    62221 cataaatcgc aagaaatagg gaatctgatt cgtgtttata accgttcaaa aagagaagag
    62281 gctgaaagtg agccacttaa tctttatgtc gaagatgaaa agggcaatct cctggcaggt
    62341 ttgatagcag agacttttgg aaattggcta gaaatcgagt atttgtttgt aaaagaggaa
    62401 ctgcgagggc aaggaatcgg ttcaaaacta ttgcaacagg cagaaagtga agctaagaat
    62461 cgaaattgtt gttttgcctt tgtcaatact taccagttcc aggcaccgga cttttatcag
    62521 aaacatggct acaaggaagt tttttctttg caagactatc tctacattag gcaaagatat
    62581 tattaccaaa agaatctgta aggtgagtat ggaggtataa tacaaagaga ggctcgcaca
    62641 taagtatgga ggtggactga tgtatatgag taactatcga taaataccag ttataaccta
    62701 gaaattgtgt ctttctagac gatattgagg acaatataat cgtagcccca aaattaggaa
    62761 tcaagactta tcaggttaag aaaagaagtg atgttgttga tattttaaac aatttattta
    62821 aactaaaaac tctctatcta ttaatccgag attcccctct cggattttgt gttgctttca
    62881 ttaaatttac aggtaagact tgctagcttc agtaaatata cacaagtttt tcagaataga
    62941 gtgaaatcca aaagaaaatg gaaataatga aagctcttgt aggcataaga aaacacctct
    63001 gtgttatact tgttgttcaa ccacaaacac aagaaagg
//
LOCUS       DAVHEW010000003        50469 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVHEW010000003 DAVHEW010000000
VERSION     DAVHEW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.003"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            555..788
                     /locus_tag="VVL53_000175"
     CDS             555..788
                     /locus_tag="VVL53_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4182399.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            1071..3020
                     /gene="mutL"
                     /locus_tag="VVL53_000176"
     CDS             1071..3020
                     /gene="mutL"
                     /locus_tag="VVL53_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU4182400.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3345..3812)
                     /locus_tag="VVL53_000177"
     CDS             complement(3345..3812)
                     /locus_tag="VVL53_000177"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU4182401.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3813..5018)
                     /locus_tag="VVL53_000178"
     CDS             complement(3813..5018)
                     /locus_tag="VVL53_000178"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU4182402.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAKMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5038..5673)
                     /gene="ribE"
                     /locus_tag="VVL53_000179"
     CDS             complement(5038..5673)
                     /gene="ribE"
                     /locus_tag="VVL53_000179"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU4182403.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5658..6758)
                     /gene="ribD"
                     /locus_tag="VVL53_000180"
     CDS             complement(5658..6758)
                     /gene="ribD"
                     /locus_tag="VVL53_000180"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU4182404.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKVFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(6892..7006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7163..7756
                     /gene="ruvA"
                     /locus_tag="VVL53_000181"
     CDS             7163..7756
                     /gene="ruvA"
                     /locus_tag="VVL53_000181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU4182405.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7766..8329
                     /locus_tag="VVL53_000182"
     CDS             7766..8329
                     /locus_tag="VVL53_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU4182406.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8326..9003
                     /locus_tag="VVL53_000183"
     CDS             8326..9003
                     /locus_tag="VVL53_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4182407.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9140..10171)
                     /locus_tag="VVL53_000184"
     CDS             complement(9140..10171)
                     /locus_tag="VVL53_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU4182408.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(10426..11109)
                     /locus_tag="VVL53_000185"
     CDS             complement(10426..11109)
                     /locus_tag="VVL53_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU4182409.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILTSMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11121..12065)
                     /locus_tag="VVL53_000186"
     CDS             complement(11121..12065)
                     /locus_tag="VVL53_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU4182410.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12197..15028
                     /gene="uvrA"
                     /locus_tag="VVL53_000187"
     CDS             12197..15028
                     /gene="uvrA"
                     /locus_tag="VVL53_000187"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4182411.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15021..16082
                     /locus_tag="VVL53_000188"
     CDS             15021..16082
                     /locus_tag="VVL53_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU4182412.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16214..16612
                     /gene="spx"
                     /locus_tag="VVL53_000189"
     CDS             16214..16612
                     /gene="spx"
                     /locus_tag="VVL53_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU4182413.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16678..17247
                     /locus_tag="VVL53_000190"
     CDS             16678..17247
                     /locus_tag="VVL53_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU4182414.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17334..17600
                     /locus_tag="VVL53_000191"
     CDS             17334..17600
                     /locus_tag="VVL53_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU4182415.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17604..18023
                     /gene="ruvX"
                     /locus_tag="VVL53_000192"
     CDS             17604..18023
                     /gene="ruvX"
                     /locus_tag="VVL53_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU4182416.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18039..18344
                     /locus_tag="VVL53_000193"
     CDS             18039..18344
                     /locus_tag="VVL53_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU4182417.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18603..19853
                     /locus_tag="VVL53_000194"
     CDS             18603..19853
                     /locus_tag="VVL53_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU4182418.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGEFAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLACQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMRERKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLAEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            19850..20395
                     /locus_tag="VVL53_000195"
     CDS             19850..20395
                     /locus_tag="VVL53_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU4182419.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTLDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            20541..22073
                     /gene="cls"
                     /locus_tag="VVL53_000196"
     CDS             20541..22073
                     /gene="cls"
                     /locus_tag="VVL53_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU4182420.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22151..22294
                     /locus_tag="VVL53_000197"
     CDS             22151..22294
                     /locus_tag="VVL53_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182421.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            22331..23869
                     /locus_tag="VVL53_000198"
     CDS             22331..23869
                     /locus_tag="VVL53_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU4182422.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            23989..26196
                     /gene="nrdD"
                     /locus_tag="VVL53_000199"
     CDS             23989..26196
                     /gene="nrdD"
                     /locus_tag="VVL53_000199"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU4182423.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKCLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26208..26348
                     /locus_tag="VVL53_000200"
     CDS             26208..26348
                     /locus_tag="VVL53_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182424.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26392..26898
                     /locus_tag="VVL53_000201"
     CDS             26392..26898
                     /locus_tag="VVL53_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182425.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            26891..27481
                     /gene="nrdG"
                     /locus_tag="VVL53_000202"
     CDS             26891..27481
                     /gene="nrdG"
                     /locus_tag="VVL53_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU4182426.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27478..28095
                     /locus_tag="VVL53_000203"
     CDS             27478..28095
                     /locus_tag="VVL53_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU4182427.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28362..28670
                     /gene="rpsJ"
                     /locus_tag="VVL53_000204"
     CDS             28362..28670
                     /gene="rpsJ"
                     /locus_tag="VVL53_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU4182428.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            28887..29513
                     /gene="rplC"
                     /locus_tag="VVL53_000205"
     CDS             28887..29513
                     /gene="rplC"
                     /locus_tag="VVL53_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU4182429.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29538..30161
                     /gene="rplD"
                     /locus_tag="VVL53_000206"
     CDS             29538..30161
                     /gene="rplD"
                     /locus_tag="VVL53_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU4182430.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30161..30457
                     /locus_tag="VVL53_000207"
     CDS             30161..30457
                     /locus_tag="VVL53_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU4182431.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30475..31308
                     /gene="rplB"
                     /locus_tag="VVL53_000208"
     CDS             30475..31308
                     /gene="rplB"
                     /locus_tag="VVL53_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU4182432.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31412..31693
                     /gene="rpsS"
                     /locus_tag="VVL53_000209"
     CDS             31412..31693
                     /gene="rpsS"
                     /locus_tag="VVL53_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU4182433.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            31705..32049
                     /gene="rplV"
                     /locus_tag="VVL53_000210"
     CDS             31705..32049
                     /gene="rplV"
                     /locus_tag="VVL53_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU4182434.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIATLTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32062..32715
                     /gene="rpsC"
                     /locus_tag="VVL53_000211"
     CDS             32062..32715
                     /gene="rpsC"
                     /locus_tag="VVL53_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU4182435.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            32719..33132
                     /gene="rplP"
                     /locus_tag="VVL53_000212"
     CDS             32719..33132
                     /gene="rplP"
                     /locus_tag="VVL53_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4182436.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33142..33348
                     /locus_tag="VVL53_000213"
     CDS             33142..33348
                     /locus_tag="VVL53_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU4182437.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33373..33633
                     /gene="rpsQ"
                     /locus_tag="VVL53_000214"
     CDS             33373..33633
                     /gene="rpsQ"
                     /locus_tag="VVL53_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU4182438.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            33659..34027
                     /gene="rplN"
                     /locus_tag="VVL53_000215"
     CDS             33659..34027
                     /gene="rplN"
                     /locus_tag="VVL53_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU4182439.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34105..34410
                     /gene="rplX"
                     /locus_tag="VVL53_000216"
     CDS             34105..34410
                     /gene="rplX"
                     /locus_tag="VVL53_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU4182440.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34434..34976
                     /gene="rplE"
                     /locus_tag="VVL53_000217"
     CDS             34434..34976
                     /gene="rplE"
                     /locus_tag="VVL53_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4182441.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            34994..35263
                     /gene="rpsN"
                     /locus_tag="VVL53_000218"
     CDS             34994..35263
                     /gene="rpsN"
                     /locus_tag="VVL53_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU4182442.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35548..35946
                     /gene="rpsH"
                     /locus_tag="VVL53_000219"
     CDS             35548..35946
                     /gene="rpsH"
                     /locus_tag="VVL53_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4182443.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36138..36674
                     /gene="rplF"
                     /locus_tag="VVL53_000220"
     CDS             36138..36674
                     /gene="rplF"
                     /locus_tag="VVL53_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU4182444.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGSKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            36758..37114
                     /gene="rplR"
                     /locus_tag="VVL53_000221"
     CDS             36758..37114
                     /gene="rplR"
                     /locus_tag="VVL53_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU4182445.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37132..37626
                     /gene="rpsE"
                     /locus_tag="VVL53_000222"
     CDS             37132..37626
                     /gene="rpsE"
                     /locus_tag="VVL53_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU4182446.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37598..37822
                     /gene="rpmD"
                     /locus_tag="VVL53_000223"
     CDS             37598..37822
                     /gene="rpmD"
                     /locus_tag="VVL53_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU4182447.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            37967..38407
                     /gene="rplO"
                     /locus_tag="VVL53_000224"
     CDS             37967..38407
                     /gene="rplO"
                     /locus_tag="VVL53_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU4182448.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38420..39730
                     /gene="secY"
                     /locus_tag="VVL53_000225"
     CDS             38420..39730
                     /gene="secY"
                     /locus_tag="VVL53_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU4182449.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            39881..40519
                     /locus_tag="VVL53_000226"
     CDS             39881..40519
                     /locus_tag="VVL53_000226"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU4182450.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40636..40854
                     /gene="infA"
                     /locus_tag="VVL53_000227"
     CDS             40636..40854
                     /gene="infA"
                     /locus_tag="VVL53_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU4182451.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            40879..40995
                     /gene="rpmJ"
                     /locus_tag="VVL53_000228"
     CDS             40879..40995
                     /gene="rpmJ"
                     /locus_tag="VVL53_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU4182452.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41013..41378
                     /gene="rpsM"
                     /locus_tag="VVL53_000229"
     CDS             41013..41378
                     /gene="rpsM"
                     /locus_tag="VVL53_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU4182453.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41396..41779
                     /gene="rpsK"
                     /locus_tag="VVL53_000230"
     CDS             41396..41779
                     /gene="rpsK"
                     /locus_tag="VVL53_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU4182454.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            41822..42757
                     /locus_tag="VVL53_000231"
     CDS             41822..42757
                     /locus_tag="VVL53_000231"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU4182455.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            42769..43155
                     /gene="rplQ"
                     /locus_tag="VVL53_000232"
     CDS             42769..43155
                     /gene="rplQ"
                     /locus_tag="VVL53_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU4182456.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTESRRGDAAPMAIIELV"
     gene            43420..43686
                     /locus_tag="VVL53_000233"
     CDS             43420..43686
                     /locus_tag="VVL53_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU4182457.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            43696..45033
                     /locus_tag="VVL53_000234"
     CDS             43696..45033
                     /locus_tag="VVL53_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU4182458.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            45277..45969
                     /locus_tag="VVL53_000235"
     CDS             45277..45969
                     /locus_tag="VVL53_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4182459.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGEIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(46072..47717)
                     /locus_tag="VVL53_000236"
                     /pseudo
     CDS             complement(46072..47717)
                     /locus_tag="VVL53_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(47707..48798)
                     /locus_tag="VVL53_000237"
     CDS             complement(47707..48798)
                     /locus_tag="VVL53_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182460.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTIRDSVYLGLNTDYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(48811..49827)
                     /locus_tag="VVL53_000238"
     CDS             complement(48811..49827)
                     /locus_tag="VVL53_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4182461.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKVELIQAGTGELFKKLESEKEVPVADVIFGGSYTQYTTHGELFENYTS
                     KENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMNIEGYNDLFKPELKGKIATAD
                     PANSSSTFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGSSSSSVYKVVADGEMA
                     VGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQE
                     VQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDI
                     QSKQ"
BASE COUNT        15244 a         9464 c        11030 g        14731 t
ORIGIN      
        1 tagtttagtt gcaggaacgc ttattgattt gaatattgaa ttagtgttaa ttgctattag
       61 cataccttat tttttgtttt cttatatttt ttatacggat aatggattga aagaatttag
      121 tatatattag aaatgtttat gttcattcaa aagcataatg actataactg aaaaagaaaa
      181 gtgatatctt taaggttgtt cttcttggtg gtgagattcg tgagacaacc caagcttttg
      241 tcggaaagat taccaatgct ttgatggata ggatgtactt tagcaagatg tttttagtgg
      301 taacggtatc gtggatggac gtgtaataac ctcttctttc gaggagtatt ttactaaaaa
      361 actagccttg gagcgttccc cagaaacgga cttactcatt gactcttcaa agatttgggg
      421 agaagatttt gcttcatctg ttccttgaaa aaagtcacag cagtcatcac agacgatagt
      481 actgaacaaa actatgaaga gttagaaatt tatacgcagg tgattgtata aaggatctgg
      541 aaatagataa gaagttgatt agtattgacc taggtggtac aaatattaag attactgttc
      601 tttcaaatga cggtgagatt gaaactttgt ggagtattac aacagataca agtgagaaag
      661 gttctcaaat tatatcggac atcatcagtt ctattaaaaa taaattgacc gaacggaata
      721 ttcctgatag cgaccttctt ggaatcggta tgggaagttg ctcatcatac tttccttgta
      781 aatcataggg gctataaact ctccgtctac ttgtcctgca acaattgaag tctgctcaaa
      841 acgccgtccg ctaatctttt catagacttt ctccctttta ggagcctagc tttctagttt
      901 gttctttgat ttttattgag tataccacta ttttactccc tctggcaagg gactttgtct
      961 atgtggaggg attgggctcc tatgtggtgg agcttttctg ttctttctga aatatggtat
     1021 aatagcacta atcaatttct aggaaaatag atacagaaag gggctgaaag atgtctcata
     1081 ttattgaatt gccagagatg ctggcaaacc aaatcgcggc tggagaggtc attgaacgtc
     1141 ctgccagtgt ggtcaaagag ttggtagaaa atgccattga cgcgggctct agtcagatta
     1201 tcattgagat tgaggaagct ggtctcaaga aggttcaaat cacggataac ggtcatggaa
     1261 ttgcccacga tgaggtggag ttggccctgc gtcgccatgc gaccagtaag ataaaaaatc
     1321 aagcagatct ctttcggatt cggacgcttg gttttcgtgg tgaagccttg ccttctattg
     1381 cgtctgttag tgtcttgact ctgttaacgg cggtggatgg tgctagtcat ggaaccaagt
     1441 tagtcgcgcg tgggggtgaa gttgaggaag tcatcccagc gactagtcct gtgggaacca
     1501 aggtttgtgt ggaggatctc tttttcaaca cgcctgcccg tctcaagtat atgaagagcc
     1561 agcaagcgga gttgtctcat atcattgata ttgtcaaccg tctgggcttg gcccatcctg
     1621 agatttcttt tagcttgatt agtgatggca aggaaatgac gcggacagca gggactggtc
     1681 aattgcgcca agcaatcgca gggatttacg gtttggtcag tgccaagaag atgattgaaa
     1741 ttgagaactc tgacctagat ttcgaaattt caggttttgt gtccttgcct gagttgactc
     1801 gggctaaccg caattatatc agcctcttca tcaatggccg ttatattaag aacttcctgc
     1861 tcaatcgtgc tattttggat ggttttggaa gcaagcttat ggttggacgt tttccactgg
     1921 ctgtcattca catccatatc gacccttatc tagcggatgt caatgtgcat ccaactaagc
     1981 aagaggtgcg gatttccaag gaaaaagaac tgatgactct ggtttcagaa gctattgcaa
     2041 atagtctcaa ggaacaaacc ttgattccag atgccttgga aaatcttgcc aaatcgaccg
     2101 tgcgcaatcg tgagaaggtg gagcaaacta ttctcccact caaagaaaat acgctctact
     2161 atgagaaaac tgagccgtca agacctagtc aaactgaagt agctgattat caggtagaat
     2221 tgactgatga agggcaggat ttgaccctgt ttgccaagga aaccttggac cgattgacca
     2281 agccagcaaa actgcatttt gcagagagaa agcctgctaa ctacgaccag ctagaccatc
     2341 cagagttaga tcttgctagc atcgataagg cttatgacaa actggagcga gaagaagcat
     2401 ccagcttccc agagttggag tttttcggac aaatgcacgg gacttatctc tttgcccaag
     2461 ggcgagatgg actttacatc atagatcagc acgctgctca ggaacgggtc aagtacgagg
     2521 agtaccgtga aagcattggc aatgttgacc aaagccagca gcaactccta gtgccctata
     2581 tctttgaatt tcctgcggat gatgccctgc gtctcaagga aagaatgcct ctcttagagg
     2641 aagtgggcgt ctttctagca gagtacggag aaaatcaatt tattctacgt gaacatccta
     2701 tttggatggc agaagaagag attgaatcag gcatctatga gatgtgcgac atgctccttt
     2761 tgaccaagga agtttctatc aagaaatacc gagcagagct ggctatcatg atgtcttgca
     2821 agcgatctat caaggccaat catcgtattg atgatcattc agctagacaa ctcctctatc
     2881 agctttctca atgtgacaat ccctataact gtcctcacgg acgtcctgtt ttggtgcatt
     2941 ttaccaagtc ggatatggaa aagatgttcc gacgtattca ggaaaatcac accagtctcc
     3001 gtgagttggg gaaatattaa aagtataaaa aagtctggga aaaattttca aaatcaaaaa
     3061 aacgcataaa atcaggtgtt caaaaacctt gattttatgc gttttatcat ggaaatagtt
     3121 acttcatttt ttcctaattc ttttcgaaac tctttttaaa cgacgtcagt tttatcagta
     3181 atctcaaaac agtgttttga gctaattttg ccagttttgt ctgtaacatc gaagttgtgt
     3241 tttaccactc tgcgactggt ttcctagttt gctctatgat tttcacagag cattaaattg
     3301 cgattttgcc aagtttcttt attcgtctaa aagtagagtc tgttctatgc gtctaatgta
     3361 cgaatcaggt tgaccatttc aatagctcct tgtgcacact cagaaccctt atttcctgct
     3421 ttagtaccag ctcgttctat ggcttgttca attgtatctg tcgttagcac accaaacata
     3481 acaggaattt cgctatttaa actgatttgg gcgattccct tagatacctc gctacataca
     3541 taatcataat gacttgtatt ccctctaatg acagctccca agcagataat tgcatcatat
     3601 tttttacttt ttgccatttt tgatgcaatc agtggtattt caaaagctcc tggaacccag
     3661 gctacctcta tatctttctc gtttacattc tctcttttga gattatctag tgctccagat
     3721 aataattttg aagttataaa ttcattaaat ctcgctacaa caatacctat tttaatattg
     3781 tttgctacta aattaccttc ataagtgttc atttattttt cctccatatt taaaatgtga
     3841 cccattcgat ttttctttgt ttctaaataa aaactatcgt aaggattggc ttctatttcg
     3901 attgatattc tactggaaat ggtaattcca tatttttcta actgttcaac cttgtcagga
     3961 ttatttgtca gtaaatgaag tgactgaagt cccagatctt taagcatttt tgctccaata
     4021 tgatattctc ttaaatcacc ttcaaagcct aatgcaagat tggcatcaag cgtatccatg
     4081 ccttgatctt gtaaatgata ggcttttaat ttattgataa gtccaattcc tcgtccctcc
     4141 tgtcgcaagt aaagtaagac acccgaacca ttctcaacaa tcattttcat agccttatcg
     4201 aattgctgtc cacaatcgca acgtaaagag cctaaaacat ctcctgttaa acattcggag
     4261 tggacccgac ataatacatt ggcttcatcc tctatatttc ccataataag agcaagatga
     4321 tgttccccat ttagtttatc tatatagcta attgctttga aattaccgta tctagtaggc
     4381 atattgacag ttgaaactcg ttctaccagc tgatcatata cttttctata ttcttgtaat
     4441 tctttgatgg taattagtgg aatgttgtgt tttttcgaga actgaattaa atcatctgtt
     4501 ctcatcattt tgccatcatg attcattatt tcacaacata ggccacactc ttttagtcca
     4561 gctaatttta ataaatcaac agttgcttct gtgtgtccat ttctttctag gacaccacct
     4621 ttttttgcaa ttaaaggaaa catgtgtcct ggcctgcgaa aatcagaggg tgttatatct
     4681 tcagctacac acatacgtgc ggtcagtcct ctttcctcgg cagaaatacc tgtggtcgtt
     4741 tctttataat caattgaaac tgtaaaagca gtcttatgat tatctgtatt gttttcaacc
     4801 ataggtgaaa gcattaattg attagctaaa ctttcgctca taggcataca aattaatcct
     4861 ttggcataag tagccataaa attaacattt tctgttgtag ctgcttgtgc agaacaaatt
     4921 aagtctcctt cattttctct atccttgtcg tctataacaa gaacaagtcg tcccttctgc
     4981 aatgcttcta atgcttcttg tatttttcga tattccattg actgattatc ctttctgcta
     5041 aaatccattt tgatataata gttccttaga tatttctgat tttggagagt tatccatcag
     5101 tttttgcaca tatttaccta agatatcatt ttcaagattt actgtactcc cgacttgttt
     5161 actcttaaga atggtttgtt ccaaggtatg agggataaca gatactgaaa agtttacttt
     5221 ggagacttta gcgacagtca gactaatgcc gtcaattgta atagatcctt tttcaactat
     5281 taaatctaaa atttcttttt gtgtgttgat ttgataccat acagcattat catctttttt
     5341 tattgacgag atttttcctg taccatcaat gtgtcctgta acgacgtgac ccccaagtcg
     5401 accgttgaca gataaggctc tttctagatt cacctcactt ccatgtttta atagagtaag
     5461 agctgttcga ctccatgttt cattcattac atcaactgta aaggattgat gattgaaatg
     5521 agtaactgta agacagatac catttactgc tatactatcg cctaaatgga tatccgttaa
     5581 tatttttgag gctttaattg atagtttaca attacgagag tctttctgta ttctttcaac
     5641 ttttccgatt tcttcaatta ttcctgtgaa catggataaa tcacttcact ttctatgaga
     5701 tagtcatttc ctatttgaga aaatgcataa ggtttcaatc taatagcgtc atttggcaaa
     5761 gaaatacctt cacctccgac aggaaacttg gcactacctc caaaaacttt tggtgcaata
     5821 tatattttca gctcatcaac aatttgttgt tccaaagcac tccaattcat tagactgccc
     5881 ccttctagaa ctaggctatc aatctgcatg tttcctagat gttgcattaa actcgataag
     5941 tctatatgat tgcctttttt ctttatggaa agtatttcac agccatgatt ttgatatagc
     6001 ttcattttat ttttgtcttc agaggaagtg gcaatgtaag ttttaatatc atttgctgtt
     6061 tttacgattt tagaggtaag aggagttcgt aaatgtgtat cgcatatgat acggatagga
     6121 tttttccctt cctccaatct acatgtcagc aaaggatcgt cttgaataac agtattgact
     6181 cccaccataa ttgcactaac atggtgtcgt aactgatgca catgctttct tgcttcttct
     6241 tcagtaatcc atttggattg atttgtttta gtggctattt ttccatccat tgacattgca
     6301 tatttcataa aaacataggg tacatgctgg gtaatatact ttctaaaact ttttattaag
     6361 ttaagacact cattttctaa aattccaaca gtaacttgaa gattattttc ctcaagtatc
     6421 tttactcctt ttccagatac aataggatta cagtctaggc ttccaatgac tactcttgta
     6481 ataccactat cgattatagc atctatacag ggaggtgttt tcccgaagtg acaacagggt
     6541 tcaagtgtta cataaagcgt cgctccgaca ggggattctc tacagttttt aagagcattt
     6601 ctctcagcat gtgggccacc aaaaaactca tgataacctt gtccgataat gtgattatct
     6661 tttacaataa ctgcgccgac cataggattg ggattgacgt aaccagcccc tttttgtgcc
     6721 agttttattg ctaatttcat atattttgaa tcgctcatct cgctacctcc aaaaaaatat
     6781 accttgaata ggggactact caaggcatac aaaagaaaac ttatgcgatt aacaaaaatg
     6841 ctctgaaatg acaagtaatc atttcagagc acgcaaaaag cacaaatata cttttatctt
     6901 ctttcatcca gactatactg tcggctttgg aatttcacca aatcatgcct ttcggctcgt
     6961 gggctatacc accggtaggg aatttcaccc tgccctgaag atagttattc aattacagat
     7021 gattatagta cttaattttg aatatgtcaa cagataaata ccgattgttt ttgatatact
     7081 gtatttgtga taatcgattc tcgctcctcg gataaagaaa atatgatata ctagataaac
     7141 gaaataagag agaaggaata ctatgtacgc atatttaaaa ggaatcatta ccaaaattac
     7201 tgccaaatac attgttcttg aaaccaatgg tattggttat atcctgcatg tggccaatcc
     7261 ttatgcctat tcaggtcagg ttaatcagga ggctcagatt tatgtgcatc aggttgtgcg
     7321 tgaggacgcc catttgcttt atggatttcg ctcagaggat gagaaaaagc tctttcttag
     7381 tctgatttcg gtctctggga ttggtcctgt atcagctctt gctattatcg ctgctgatga
     7441 caatgctggc ttggttcaag ccattgaaac caagaacatc acctacttga ccaagttccc
     7501 taaaattggc aagaaaacag cccagcagat ggtgctggac ttggaaggca aggtagtagt
     7561 tgcaggagat gaccttcctg ccaaggtcgc agtgcaagca agtgctgaaa accaagaatt
     7621 ggaagaagct atggaagcca tgttggctct gggctacaag gcaacagagc tcaagaaaat
     7681 caagaaattc tttgaaggaa cgacagatac agctgagaac tatatcaagt cggcccttaa
     7741 aatgttggtc aaataggagc agagaatgac aaaacgttgt tcgtgggtca agatgaccaa
     7801 cccgctctac atcgcctatc atgatgagga gtggggccag cccctccatg atgaccaagt
     7861 attgtttgag ttgttgtgta tggaaaccta tcaggcaggc ctgtcttggg aaacggtact
     7921 caacaaacgc caagctttcc gagaagtctt tcatagctat caaattcact cagtcgcaga
     7981 gatgactgac actgaattgg aagccatgct ggagaatcca gctatcattc gaaatagagc
     8041 caagcttttt gctacacgcg ctaacgccca agcctttcta cagttacagg cagagtacgg
     8101 ctcttttgat gcctatcttt ggtcttttgt tgaggggaaa actgtcgtta acgatgttcc
     8161 tgattatcgc caagcgccag ctaaaacacc cttatctgag aaattagcca aagatctcaa
     8221 aaaacgaggc ttcaagttca caggcccagt cgccgtattg tcttttctac aggctgcagg
     8281 gctagttgat gaccacgaga atgattgtga gtggaaaggt cttaaatgat gtctaacaaa
     8341 aataaggaaa ttctgatttt tgcgattctc tatacagtcc tctttatgtt tgatggcgtt
     8401 aaattgctgg cttctttaat gccatctgcc attgcaaatt atcttgttta tgtagtttta
     8461 gctctatatg gctccttctt gttcaaggat agattgatcc aacaatggaa ggagattaga
     8521 aagactaaaa gaaaattctt ctttggagtc ttaacaggat ggctctttct cattctgatg
     8581 actgttgtct ttgaatttgt atcagagatg ttgaagcagt ttgtgggact agatggacaa
     8641 ggtctaaatc agtctaatat tcaaagtacc tttcaagaac aaccactact gatagctgtt
     8701 tttgcttgtg tcattggacc tctggtagaa gaattatttt tccgtcaggt cttattgcat
     8761 tacttgcagg aacggttgtc aggtttacta agcattattc tggtaggact tgtttttgct
     8821 ctgactcata tgcacagttt ggctctatca gagtggattg gtgcagttgg ttacttaggt
     8881 ggaggccttg ccttttctat tatttatgtg aaagaaaaag agaatatcta ctatccccta
     8941 cttgttcaca tgttaagcaa cagcctctcc ttaatcattt tagctatcag tatagtaaaa
     9001 tgaaatgaga acaggacaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt
     9061 ctagtttcaa tatactgtaa tatgtgatga aaatgccagt aatgataccg agaaaaaagc
     9121 tgagaaactt ttcccagctt tatttgttat agtcaaagag aatgacttgt tcctgtgcat
     9181 ctacatgagc atggacccca aagggtacaa ttgctcttgg agttgcgtgg ccgacattca
     9241 gattatagac aatcgggata ttgctgtcaa tgatatccaa tagtgcctct ttatagtcgt
     9301 catggaaagt ttcatccata ggttttccga ccaagagtcc attgatgacc gcgaatatgc
     9361 cagtgtcctt taaagttagc aacatctttt tgaagtcttc tggcttaggc ttttcttcgc
     9421 ttgtttcgag caagaggatt ttcccttccc agtctgacaa gtcagggaaa agtttgtatt
     9481 tttggcagag ttccgtgcta tctgcgtatc gagagttgtc aaagatatcg tagagggatt
     9541 cgaggcaacc accgaggatt ttcccctcga actgggcact tccttgcaac aagtcaaaac
     9601 ctgtatttgt atgactgaca cgaggtgttc ccagggccgt gggactaaaa tcagttcgtt
     9661 cctcatacca aacgtcacta gggcggattt ctgaaattct tcccgtctca atcaattctt
     9721 taaagtagtg aaggctatag gctagcattt ctttgtctaa ttcacaaatg tctgctaaaa
     9781 aggattgacc ataaaaagtc ttgattccta atttatgcaa catgaggtgg ttcatggttg
     9841 tatccgagaa gccaagaaaa attttttgtt tgataacctt ttggagttgg tcattttcaa
     9901 aaagataagg tagcaagcga taggtatcgt ctccaccgat ggcacatagg atcatgtcga
     9961 tgctatcatc agaaaaggca tgaatcaaat cctctgcacg agcttcagga tggtccttga
    10021 taaagtctaa tccttttaac gaatggggca aaaagatggg attgagtccc agatccttga
    10081 gacgttggat acccaagtcc acttcgtgtt tgacaaaatc ctctccgata atgccactag
    10141 ataaactaac aataccaata gtagaaacca tatctcatcc tcctagaaat agattgagcc
    10201 tattttatca caaaatataa gagaaagcta tgtgggattt cagaagaata cttttaatac
    10261 tcttcgaaaa tctcttcaaa ccacgtcagt tttatttgca acctcaaagc tgtgctttga
    10321 gcaacctgca gctagcttcc tagtttgctc tttgattttc attgagtata actcaaaaaa
    10381 gtaagtgaaa aagcaaccgc acctgcagtt gcttttatcg ttttcttatt cttgatagcg
    10441 tgttggtcct gcacttgcac tacggatatt caagcgtttc ttgagataga agcgaagggc
    10501 taggagggct actccaataa tagctagtgg caatggatcc agtactgggt taaggctagc
    10561 tggtaggaag cttgttgcaa agaagacaag caaccaaagg aacatagaag ttaggataac
    10621 tagtacagat ttccagaaag gtggacgttg actgcgatcc atatctggtc catagtattg
    10681 gtaaacaaag tagtacatca agtagaaggc aaatccacca accagtccaa ctaatagaag
    10741 agtaatcaat ccatagccaa aagcttgatc tgccgcaaag aaggttgtga gggcgctgac
    10801 aagggcaaag aggctagtga tgaaaagagc tgagtccata atcatcagtt ttggatcatc
    10861 attttctttt ggatgctctt tttcgtactg ctctttgaca gtgaagctat gagcccaatg
    10921 agttggtgcg ccgtataggg aacgggcagt tgtaccttta gattgctcct caaggatttg
    10981 gggaatgact tcctcaaaaa tagtctggat ttcagcgtct gttttcccat ctttgatgaa
    11041 ttgttgggta gcaatgtgga caaactcttg gtttttctta gttaattttt gtagatcaat
    11101 ttgagacata ggaactcctc ttagaaccat tttttatgga tgagatagag agtgagcgag
    11161 acactcatag caaaggcgat aaagacgatt aaccagaagg catttggctc tccgtttagg
    11221 ggaatttcat tatccttaaa gttcatcccg taggcagaaa agaccatggt tgggatggac
    11281 atgacgatgg tcacaagggc caaggttttc atgatgttgt tctggttgtt agaaatgata
    11341 gaggcaaagg tctctgtcat agaatgcaag acgtttccat aaatatctgc catctcgatg
    11401 gcctgttggg tttcaatcag ggtgtcttca agcagatctt cgtcctcaag gtatttcttg
    11461 atattgctgg ttgaactggt caatttctta atcacgcgct catttgtttt gagggaggcc
    11521 ttgaaataga cgatagtttt ttccaattcc atgagctcaa tcaattcttc attacgagtt
    11581 gattgatgca gttgactttc gatttgttca ctcttgcggt cgattgaacg aagggctgtt
    11641 aggtaaagct ctgcattgcg ataaagaatt tgaaagataa aacgtgaacg catgaaggta
    11701 tagaaattac gcaatcgacg gttgataaag acatcaagga caggtagtgg ttccaaacac
    11761 gtagtgataa tggtttcctc agtgatgata ataccaagcg ggatggttac gtagtaggtg
    11821 cggttatttc tttcctccgt gaccggcacg tctacgataa tcagggtata ctcgtcttca
    11881 atggtaatac gagacatttc ttccgcatcg agcggtgctc gaaggtcggc aatatcaata
    11941 tcgaaggtgt tggcgatttc gagtgattca ttttgagtcg gattgacgag attgatccaa
    12001 gtacccggtt caagcgtatc gatctcttta aattcagttg ttgtagagag aaaaacttgt
    12061 ttcatatccc ttatcctttc tcatttttca gattttttca cactgtacta ttatactaca
    12121 aaatcggcct tttgggtaat agaatctcac tttttttggt ataatggtaa gcaataatgg
    12181 actagaaaga acaaagatgc aagataaaat tgtcattcat ggggcgcgtg cccataattt
    12241 aaaaaatatt gatgtggaga ttccgcgaga caagttggtt gtcgtgactg gcttgtcagg
    12301 ttcagggaag tccagtctgg cttttgatac tctctatgcg gagggacaac gtcgctatgt
    12361 agagagtttg tctgcctacg ctcgtcagtt cttgggaaat atggaaaaac cagatgtaga
    12421 tgccattgat ggtctcagcc cagctatttc catcgaccag aaaacgacta gtaaaaatcc
    12481 tcgctcgacg gtgggaacga cgactgaaat caatgactat ctgcgtctcc tctacgcacg
    12541 tgtggggacg ccttactgta tcaacggaca tggagctatc aatgcttctt ctgtggagca
    12601 aatcgttgat aaggttttgg agttacctga acgccagcgc ttgcagatct tggctcctgt
    12661 catccgcaag aaaaaaggcc aacataagag tgttatcgag aaggttcaga aagacgggta
    12721 tgttcgtgtt cgtgtggatg gggaagtcta tgatgtgacc gaagtgccag agttgtctaa
    12781 gagcaagcaa cacaatatcg atgtcgtggt tgaccgtatt gttatcaagg agggcattcg
    12841 tagccgtctc tttgattcca ttgaagctgc ccttcgtatc gcagaaggtt atgtgattat
    12901 cgacaccatg gacgactctg agttgttgtt ctctgagcat tatgcctgtc cagtttgtgg
    12961 atttactgtc ccagagttag aacctcgtct cttctctttc aatgctccct ttggctcttg
    13021 tagtgagtgt gacggcttgg ggattaagct ggaggtggat actgatttgg tagtgccaga
    13081 tgccagcaaa accttacatg agggagcgct ggcaccttgg aatcctatct catccaacta
    13141 ctatccaaac atgttagagc aggccatgaa agtctttgga gtggctatgg ataagccttt
    13201 tgaggacctg tcagaagaag ataagaactt gattctctac ggctcagatg gtaaggaatt
    13261 ccatttccat tatgaaaatg aatttggtgg tgtgcgcgat atcgacattc cgtttgaggg
    13321 agttatcaat aatatcaaac gtcgttacca tgaaaccaat agcgattaca ctcgcactca
    13381 gatgcgtctc tacatgaatg agctgacctg cggaacctgt caaggctatc gtctcaatga
    13441 ccaggccttg tctgtccgtg tgggcggcca gcaagggcca catattggag aaatctcaga
    13501 cctgtctatc gctgaccact tggacttggt gagccagtta actttgtctg aaaatgaagc
    13561 catcattgct cgtcccattc tcaaggaaat caaggatcgt ttgaccttcc ttaataatgt
    13621 gggtcttaac tatctaaccc tgtcacgttc agcaggaacc ctttcaggtg gggaaagtca
    13681 gcgcattcgt ttggcaaccc agattggttc caacctatca ggtgtcctct atatcctaga
    13741 cgagccgtca atcggtcttc accagaggga caatgaccgc ctgattgcca gtctgaaaaa
    13801 gatgcgtgac ttgggcaata ctcttatcgt ggtggaacac gacgaagata ccatgcgtga
    13861 agctgattat ctgattgacg ttggtcctgg tgccggtgtt tttggtggtg agattgttgc
    13921 agcaggtaca cctaagcaag tggctcgcaa cagcaagtct atcacaggtc agtacttgtc
    13981 aggcaaacgt gtcatcccag taccagaaga gcgccgtgtc ggaaatggtc gttttattga
    14041 agtgacagga gcgcgtgaga acaacttgca aaatgtcacg gctcgctttc cactaggaaa
    14101 atttatcgca gtgacaggtg tgtcgggttc agggaaatcg actctaatca acagcattct
    14161 caaaaaagcc attgcccaga agctcaaccg taattcagac aaacctggta agtttaagac
    14221 tattacaggg attgagcatg tcgaccgttt gattgatatt gaccagagcc ctatcggacg
    14281 gacgccgagg tctaacccag ctacctatac aggagttttt gacgatatac gtgacctttt
    14341 tgctcagaca aatgaagcca agattcgagg ctacaagaag gggcgcttca gtttcaacgt
    14401 caagggaggt cgctgtgaag cttgctcagg tgacgggatt atcaagattg agatgcactt
    14461 cttgccagat gtttatgtgg cttgtgaagt ttgccacgga acccgctata acagtgaaac
    14521 cctagaagtt cactacaagg aaaagaatat ttcgcaggtc ttggatatga cggtcaacga
    14581 tgcggtagaa tttttccaac acattccgaa aattcaacgc aaactccaga ccatcaagga
    14641 tgtaggtcta ggctatgtga ccttgggaca gccagctacc accctttctg ggggagaagc
    14701 ccaacgtatg aagttagcta gcgaactcca caaacgctcg acaggaaaat ctttctacat
    14761 tctggatgag ccgacgacag ggcttcatac agaggacatt gctcgcctgc tcaaggtttt
    14821 agctcgcttt gtagacgccg gcaatacagt cctcgtcatc gagcacaatc tggatgttat
    14881 caagacggca gaccatatca ttgacttggg acctgagggc ggtgtcggtg gtggaaccat
    14941 catcgtaaca ggaactccag aagaagtagc tgccaacgaa gccagctata caggacacta
    15001 tttgaaagga aagttacatc atgaataaac gcgtacaagc atttctagct aaaatgcaag
    15061 aaaaagaact agatggtatc atcatcaaca atcttaaaaa cgtctattat ttgactggtt
    15121 tttggggctc aaacggaaca gtctttatca gccgtgaccg tcaggtctta gtgacagact
    15181 ctcgctatat catcgcagct aagcaagaaa ccagtggttt tgagattgtg gctgatcgtg
    15241 atgaattggc tgtcattgca ggaattgtta aggacatgaa cttgactcgt atcggttttg
    15301 aagatgagat ttcagtgtct tattaccacc gtatgcaggc agcttttgca ggtttgaact
    15361 tgcttccaca aactcagttt gtggaaggtc ttcgtatgat taaggatgag gcagagattg
    15421 cagcgattcg caaggcttgt tctatctcag accaagcttt ccgcgatgcg cttgacttta
    15481 tcaaaccagg aaaaactgaa attgagattg ccaacttcct tgatttccgc atgcgtgagt
    15541 tgggagcatc tggcttatct tttgatacga tcctagctag cggtatcaat tcttctaaac
    15601 cccatgccca tccaatgcac aaaccagtgg agttgggaga agccatcacc atggacttcg
    15661 gctgtctcta tgaccactat gtcagtgata tgacccggac tatctatcta gggcatgtta
    15721 gcgatgagca ggcagagatt tacaatacgg ttctaaaagc taaccaagcc ttgattgacc
    15781 aagctaaggc aggcttaggt ttccgtgact ttgacaaaat ccctcgtgat attatcattg
    15841 aggcaggtta tggtgactac tttactcacg gcattggcca cggtattggt ctggatatcc
    15901 atgaggaacc ctactttagt cagacttcta cagaaactat taagacaggt atggccttga
    15961 ccgatgaacc aggtatctat atcgaaggca aatatggcgt tcgtatcgag gatgatatcc
    16021 tgattacaga gacaggttgt gaattattga ccctagctcc aaaagagttg atagtcattt
    16081 agttgtttgt caagaaaaaa gtacgaaaat gacgaaaaaa gtcaaaaaaa attaaaaata
    16141 ggtcgcaagt cggatgtttt ttatggtata atagactaaa ctgatagtaa catgtagcga
    16201 aaggggtagg tacatgatta aaatttatac agtctcaagt tgtactagct gtaaaaaagc
    16261 aaaaacctgg ctcaatgccc accagttaag ttataaagaa caaaaccttg gtaaagaagg
    16321 aattacgaga gaagaattac tggatattct aaccaaaaca gataacggaa tagccagcat
    16381 cgtttcgtct aaaaatcgct atgccaaagc ccttggagtg gatattgaag atttgagtgt
    16441 caatgaagtt ctcaatctga ttatggaaac accgagaatt ttaaagagcc caatccttgt
    16501 agatgaaaaa cgcctgcaag ttggctacaa ggaagacgat attcgtgcct tcctaccacg
    16561 ctctgtccgt aatgtagaaa atgcagaagc acgtttgcgt gcagctctat aaacatcaag
    16621 gctgggagca cttcccagtc ttattctatt ttaatttcaa aaagaaagaa gaaagaaatg
    16681 aaaaaaatag ttcttgttag tctagctttc ctttttgtcc tggttggttg cggacagaaa
    16741 aaagaaactg gaccagctac aaaaacagaa aaagatacgc ttcagtcggc attgccagtt
    16801 attgaaaatg ctgagaagaa tacagttgta actaagactt tggtcttgcc caagtcagat
    16861 gatggtagcc agcagacaca aacaattact tacaaagaca agactttttt gagtctagct
    16921 atccaacaaa aacgtccagt ctctgatgag ttgaagactt atattgacca acatggagtg
    16981 gaggaaactc aaaaagctct tcttgaagcg gaggagaagg ataagtctat cattgaagct
    17041 cgtaaattgg caggtttcaa acttgaaaca aaactattga gcgcaacgga acttcaaaca
    17101 acgactagtt ttgattttca agttctggat gtcaagaagg cttcccagtt ggaacatctg
    17161 aagaatattg gtttggaaaa tcttttgaaa aatgaaccaa gcaaatatat ttcagataga
    17221 ttggcaaatg gcgcgacaga acaatagaaa atccaaataa atagactgcc tgaattgtag
    17281 aaggtaagga attatgctat aatggtacta ttctaaggaa agagggtgtt agaatgggat
    17341 ttactgaaga aacagtacgt tttaaattgg acgattccaa taaaaaagaa attagcgaaa
    17401 ctttgacaga tgtttatgct tcgttgaacg ataagggtta caacccaatt aaccaaatcg
    17461 taggttacgt attgagtgga gaccctgcct acgttcctcg ttataataat gcacgaaatc
    17521 aaatccgtaa gtatgagcgt gatgaaatcg ttgaggaatt ggttcgctac tacctcaaag
    17581 gacaaggagt cgatctataa cctatgagaa ttatgggatt ggacgtcggt tcaaaaacgg
    17641 taggggtggc gattagcgat ccgcttggtt ttacagctca agggcttgaa atcatccaga
    17701 taaatgaaga acaaggccaa tttggttttg accgcgttaa ggaattggtt gatacttaca
    17761 aggtggaacg atttgtagtg ggcttgccta aaaacatgaa caatacaagt ggaccgcgcg
    17821 tagaagctag tcaagcctac ggagcaaagc tagaagagtt ttttggttta ccagtagact
    17881 atcaggatga acgcttgaca acagtggctg ctgagcgcat gttgattgaa caagcagata
    17941 tcagtcgcaa taagcgcaag aaagtcattg ataagttagc agctcagctg attttacaaa
    18001 attatttaga tagaaaattt taatataaag gagaggctat gtcacacgat cataaccacg
    18061 accacgaaga acgtgaacta atcacactag tagatgagca aggaaatgaa accttgtttg
    18121 aaatcctttt gacaattgat ggaaaagaag aatttggtaa aaactatgtt cttctagtac
    18181 cagttaacgc agaagaagac gaagacggac aagttgaaat ccaagcttac tcattcatcg
    18241 aaaacgaaga tggaacagaa ggcgaattgc aaccaatccc agaagactca gaagacgaat
    18301 ggaacatgat tgaagaagtc ttcaacagct ttatggagga gtgatacagt ctgggagact
    18361 gtatcagccc aactcccaga aatggggaag agttggaacg tccagtgaac atttttaccc
    18421 caactcccag aaatggggaa gagttggaac gtccagtgga tgtttttagc ccatattcac
    18481 attcatagta gctagttatt agctaaccag gtaagaggtc gggacgaaaa tcctggcctt
    18541 gtttttatta gtaaccatac tttaatgcga taattgattg gacttttgtc aaggagatgt
    18601 gtatgtttga agtagaagaa tggctccata gtcggattgg tttgaatttt cgatcaggtt
    18661 tgggtcgaat gcagcaagcg gtggatttgt taggaaatcc tgagcagtct taccctatta
    18721 tccacgtaac agggactaat gggaaaggat ctaccattgc ttttatgagg gaattattta
    18781 tggggcatgg caaaaaagtt gcgaccttta cctcccctca tatcgtctct atcaatgacc
    18841 gaatctgcat taatgggcag cctatagcag acgcagactt tatccgtttg actgatcagg
    18901 tcaaggagat ggagaaaacg cttctgcaaa ctcctgccca gttgtccttt tttgaattgc
    18961 tgaccttggt tgcttttctt tattttaggg agcaggaggt ggatttggtt ttattagaag
    19021 tgggaattgg tggcttactt gacacgacca atgtggtaac tggagagttt gctgtcatca
    19081 cctccattgg gcttgaccat caagaaacct tgggtgatag tctagaagca attgcagagc
    19141 agaaagctgg tattttcaag gctggtaaga aggcagtgat tgcgaaattg cctccagaag
    19201 ctaggcttgc ctgtcagaaa aaagccgaat ctttagctgt taacctttat caggcaggtc
    19261 aagatttttt aatgctgaat ggtgattttt caagctcttt actaaatctt tcacagctga
    19321 acataggctt agaaggagtc tatcagcagg agaatgcagc cttggcgttg caaacttttc
    19381 ttctttttat gagagaaaga aaggaagctg ttgatgaaca ggctgtaaga aaggccttgg
    19441 aacagaccca ttgggctggt cgcttggagc gtattcgccc acagatttat ttggatggtg
    19501 ctcataacct ccctgccttg actcgcttgg ctgagtttat caaagaaaaa gagcaggaag
    19561 gctatcgacc tcaaatcctc tttggatcct tgaaacgtaa ggattatcaa gggatgttgg
    19621 gttatctgac tgaaaaattg cctcaggtgg aactcaaggt gaccggcttt gactatcagg
    19681 gggctttgga cgaaagggat gtgacaggtt acgatatagt ttcttcttac cgagaattta
    19741 tcagcgattt tgaagaaagg gcagacgctc aagacttgct gttcgttaca gggtctctct
    19801 attttatctc agaagtacgg ggctacctgc tggaccgtga gcagataaat tgactccatt
    19861 ttttgaactt gttatactag gggaattgct ggttagagaa aatttctcta aattggtagc
    19921 agaaaggaaa ttcatcatga aattaaaaag attcacactt tctcttgctt ctctagcaag
    19981 ttttagtctc ttagtagctt gttcacaaag agctcaacag gttcaacagc ctgttgctca
    20041 gcagcaggtc caacaacctg ctcaacagaa taccaatact gcaaatgcag gaggtaacca
    20101 aaatcaagcg gctccagtac aaaaccaacc tgttgctcaa ccgaccgata ttgatgggac
    20161 ttatactggt caggatgacg gagaccgtat cactttagtg gtaactggaa cgactggtac
    20221 atggactgag ctcgaatctg acggggatca gaaagtcaaa caggttacat tggattcagc
    20281 aaatcaacgc atgattattg gcgatgatgt caaaatttac actgtaaacg gtaatcaaat
    20341 cgtcgtagat gatatggata gagacccatc ggaccaaatc gttttaacta aataagacta
    20401 tgcaagtaga aagggctgga tttttcagcc tttttacttt tacctagaaa ataatcataa
    20461 atacttgcct tctaccgaat acctgcgtta tactagtatc aattacttgt ttttagcatt
    20521 caaactatca aggaggggat atgaaatata ggaaatttca attattgatg tccaagtatg
    20581 gctttagtct ttcgattatg ctacttgaac tttgtcttgt ttttggtctc tttctttatt
    20641 taggacgcat ggctcccatt ttatggatta ctgtcctcat tctactgagt atcatcacaa
    20701 tcatttcgat agtcaaccgt aatacgactc ctgagaataa ggtaacctgg ttgttagtag
    20761 cctttgtgcc agtatttggt cccttgctct atctgatgtt tggtgaaagg cgattgtcca
    20821 aaaaagaaat caaacaactg aagaagctag gctctatgca tttccaagaa gcaaatagcc
    20881 agctactaaa agagaaatta aaagaaagtg acaaggcagc ttatggagtc atcaagtcct
    20941 tattgagtat ggataccaat gctgacatct atgatcaaac tgcctctaca ttttttccta
    21001 acggagaagc tatgtggaaa aagatggtag aagatcttaa aaaggctgag aaatttattt
    21061 tcttggaata ttacattata gaagaaggtt tgatgtggaa tcgcatacta gatatactag
    21121 agcaaaaggt agctcagggt gtagaggtta agatgctcta tgatgatatc ggctgtatgg
    21181 ctactttaac aggagattat gcacatcgac ttcgtcagct gggcatcgag gcccataaat
    21241 tcaataaagt tattcctcgt ttgacagtgg cttataataa cagagatcat agaaaaatat
    21301 tgattgttga tggtcagata gcctatactg gtggggtcaa tctggcagat gagtacatta
    21361 accacgtcga gagatttggt tattggaagg atagtggaat tcgcttagac ggactagcag
    21421 taaaagctct gacacgctta tttttgacca cttggtacat taatcgagga gaaattagtg
    21481 attttgatca atatcattta gaaaatcatt ctatcccgag tgacggttta accattccat
    21541 acggaagtgg acccaagcca atttttcgag cgcaggtagg gaaaaaagtt tatcagagtt
    21601 taatcaatca agcaacagaa tcggtctata ttacgacacc ttatttgatt atagattatg
    21661 atttaacaga gacaatcaaa aatgcagcta tgagaggggt cgatgttcga attatcaccc
    21721 cttacatacc agataagaag ttcattcagt tagtcacgag aggagcttat cccgaccttc
    21781 tttctgctgg tgttcggatt tatgagtata gtccaggttt tattcatagt aagcagatgt
    21841 tggtagacga agattttgcg gtggtgggga caatcaatct cgactaccgg agcttggtac
    21901 accattatga aaatgcagtc ttactctata aaactccttc tataagggaa atcgcccgag
    21961 attttcgaaa tatatttgca gattctcagg aagtctatcc tcattctatc aaaacgagct
    22021 ggtatcaaaa gcttgtaaaa gaaatcgccc agctattcgc ccctatctta taagaaatct
    22081 aggacttagg atacaaacca gacctatttt tcttgtcttt tacgaataga aagaaagagg
    22141 aggggaattc atgctaacta aggaagaagt gaatgatatg attgagttca aacagacaca
    22201 tttacaccgc tttcgggaaa ttgaaacctt tgtgaaacta tgcaaaaaat cactcaaaca
    22261 gccatcgcaa gctgacaatc ctaggacttt ttgatatact aagacagata aattgaacag
    22321 gagaaacgat atgagtgtgt atggtagagt agaagaagtt cataaggaga atcgtgaacc
    22381 tctagaatac caaatcgaac aagaatcgca tcatcgtgaa tcaagtcgtc ttcctttggt
    22441 gaaaatttta ctatggagta cgcttgtaac ggggataact ctaggagtac cgctattact
    22501 cgacttaatg agtgcacaag aagtgcagga tttttatgca ggttgggcgc ttcatcagac
    22561 agggaagatt tacagcgact attatggaag tcaaggtttg ctttattatt tgctgactta
    22621 cgtgagtcag ggcggatttt tctttgccat ctttgagtgg ttagccttgg tagcaggagg
    22681 atttttcctt tttagatcag cggacacctt gacagagcaa ggagaccaag ctggacatct
    22741 ggtgactatt ttttacatgc tagttacagg tcttgctttt ggtggaggct atgcgactct
    22801 tttagcgctt cctttcttat tcgcagcctt tagtttagtt gcggcttacc taagcaatcc
    22861 aagccatgat aagggatttg tacggattgg gctagctttg gcaggcggat ttttctttgc
    22921 tcccttatca tcgctcctgt ttattgctgt agtgagttta ggcttgttgg tctttaacct
    22981 tgggcataga cgctttgcgc atgggtttta tcagtttctt gcagtggctt taggtttttc
    23041 acttgtcttt tatccaactg cctactatag tgctgcaaca ggaagttttg gggatgcgat
    23101 tagtggtatt cgttatccta ttgacagtat tcgctttgat tttacttcta aaattttaga
    23161 gaatatgttt ttttatggct tgttgtccct tggtttggga tttgtggttt gtatcttttt
    23221 aggtctcttt caatccaaac cctttaaact atacgtcatt tcggttcctg caagtcttgt
    23281 ggtaatttta ggtttgattt tacttttctt ttcacaagag cctctgcacg cttcttattt
    23341 gatggtcgtc ttccctgttt tcctactttt attggtaacc aatattaaga gtcaacagag
    23401 ggggcgtagt gctagaagaa gccgaagaga aacgccagtt agcctatgga gtcgtttctt
    23461 caaaggaaat ctatatctgc tagtttttgg gtttgtctat cttttgtctg ttcctttttt
    23521 gatgaagttt gtcctttatc cagtacctta tcaagaacgt aatcgtcttg ctgatttggt
    23581 aaaagaggag acaaatacgg aagatgctat ctatgcatgg gatgatactg cgactcttta
    23641 tcgtaagagt gagcgcttgt cgccatcggc gattttgtcc ccgttgcact atacagcaac
    23701 tgaggaaaat cgtaataagt tacttaatga cttgaaagaa aaacaaccta aggtgattgt
    23761 ggtaaatgat aaggtggtag tctggtctga agtggaaaca ctcttaaaag aaaattacca
    23821 acaagtaaag actgattact cagagtttaa agtctataaa attaaataac caaatcaata
    23881 tcttgtgtat ttttaaaaat tttaggattt ttaacacaag atattgattt ttctttttag
    23941 agtggtataa tactttttag aaagaacatt ttagaaaaga gcatgcatat gattgcacta
    24001 gaagaaaaaa ttacaatttt gccaactctc ttcgtcgaga aacgagatgg gagacgtgtt
    24061 gtatttgatg tggacaagat tgacaaggct ctccacaagg cggctgacaa ggttatggat
    24121 gtgacacccc tggttgaaaa atgcctcaat gatctgactg agcgaattat tacagaaatt
    24181 catagtcgct ttccacaggg aattaagatt tacgaaattc aaaatatcgt agaacatgaa
    24241 ctccttgaag ccaaagaata tgcgctggct gaggagtata ttacttatcg gacacagagg
    24301 gattttgagc gctcaaaagc gacggatatc aactttagta ttcataaact tctcaacaaa
    24361 gaccagacag ttgtcaatga aaacgctaat aaagacagtg atgtctttaa cactcagcgt
    24421 gatttgacag cagggattgt tgggaaatca atcggactgc aaatgcttcc taagcacgta
    24481 gccaatgccc accaaaaggg ggatatccac tatcacgatt tggactacag tccctatacc
    24541 cctatgacca actgctgttt gattgatttt aagggtatgt tggaaaatgg ttttaagatt
    24601 ggaaatgcag aggtagagag tcccaagtct atccagactg cgacagcaca gatttctcaa
    24661 atcattgcca acgttgcttc tagccagtac ggtggctgtt cagctgaccg tatcgatgaa
    24721 attttggcgc cttatgcaga gaagaattat caaaaacatc tcaaagatgc agaagagtgg
    24781 gtattgcctg aaaaacagga agattacgct tggaagaaag cgcaaaagga catctacgat
    24841 gccatgcaat ctcttgagta tgaaatcaat actctcttca cttcaaatgg acaaacacct
    24901 tttacttcgt taggttttgg tctgggaacc agtcgttttg aacgagaaat tcaaaaagct
    24961 attttaaaca ttcgcatcaa gggtcttggt tcagaacacc gtacggctat ctttcctaaa
    25021 cttatcttta cgcttaaaag aggcctcaac ttagaggaag gaactcccaa ctatgacatc
    25081 aagcagttgg ctctagagtg tgcaaccaag cggatgtatc cagacgtctt gtcttatgat
    25141 aagattgttg atttgacagg ttctttcaag gtgcctatgg gctgccgttc tttccttcaa
    25201 gggtggaagg atgaaaatgg tgtagaagtc aattcaggtc gcatgaatct gggtgttgtg
    25261 acggttaatc tgcctcgtat tgctcttgag tctgaaggtg atatgaataa gttctgggaa
    25321 atcttcaacg agcgaatgaa tatcgcagaa gatgctcttg tttaccgtgt cgaacgcact
    25381 aaagaggcga caccagcgaa tgctcctatt ctttatcagt acggtgcttt tggccatcgt
    25441 ctaggtaaag aagaaagtgt tgaccagctc tttaagaatc gtcgtgcgac cgtttcgctg
    25501 ggctatatcg gcttgtatga agtagcgaca gttttctttg gtaacagctg ggaaagtaat
    25561 ccagatgcta aggaattcac gctagacatc attcacgata tgaaacgccg tgtagaagag
    25621 tggtcagacc aatatggcta ccatttctct atctactcaa caccatccga aagtctgaca
    25681 gaccgtttct gccgactaga tatagacaag tttggctcta ttcctgatat cacagacaag
    25741 gaatactaca ccaactcttt ccactacgat gttcgtaaaa atccaacacc gtttgaaaaa
    25801 ttggactttg agaaagtcta tccggaagca ggtgcgtcag gtggtttcat ccattattgt
    25861 gagtatccag tccttcagca aaatccaaag gccttggaag ctgtctggga ttatgcttat
    25921 gaccgtgtag gctatctagg caccaatact ccgattgacc gttgctacaa gtgtgacttt
    25981 gaaggggatt ttgaaccaac tgagagaggg tttgcttgtc caaactgtgg caatagcgac
    26041 cctaaaacag tagatgtggt gaaacgaact tgtggctacc taggtaatcc tcaagcaaga
    26101 ccgatggtca acgggcgtca caaggaaatc gctgcgcgtg tcaaacatat gaatggttca
    26161 acgattaaaa tagctgggca tcaagtaaca aattagaaag aaatgaaatg ggaaaatatc
    26221 aactagacga taaggggcgc gcacaagtga cccgttatca cgagaaacac tctaaaggtg
    26281 gagctggtaa gaaagaacgc ttgcttagct tcagagaaca atttttaaac aagaacaaga
    26341 aaaaataaaa gtgagagcca gctctcgctt ttctcatagt gggaggtaag gatggaatta
    26401 cgcagaccaa gattagcgga taagaaagct gttttagata tgatgacaga gtttgaaaaa
    26461 tttcagtcgc ctcacgacgg cggtttctgg gatacagaga actttgtgta tgaagactgg
    26521 ttagaaagca atcaggaaca ggaaatgggg attaatctgc ctgaaggatg ggttcctgca
    26581 attcagttag tggctttttc tgagaaaggt caagcagttg gatttcttaa tctccggttg
    26641 cgcctcagta actttctact agaagaaggt ggccacattg gctactccat tcgtccatct
    26701 gaaagaggca agggttatgc aaaagagact ctccgtcagg gcttgcaagt tgctaaggaa
    26761 aagaacatca agaaagctct ggtgacctgt agtgtgaata atcctgctag cagagcagtc
    26821 attctagcaa atggtggaat atttgaggat gctcgcaatg gagtcgagcg ttattggata
    26881 gaggtagcga atgaataatc caaaaccaca agaatggaaa agcgaggaac ttagtcaagg
    26941 tcgtatcatt gactacaagg cctttaactt tgtggacggc gaaggcgtgc gcaactctct
    27001 ctatgtatca ggctgtatgt ttcactgcga gggatgttat aatgttgcga cttggtcttt
    27061 taatgctggc attccctata cagcagaatt agaagagcag attatggcag accttgccca
    27121 accctatgtt caaggcttga ctttgctggg aggggagcct tttctcaata ctgggattct
    27181 cttgccactt gttaagcgga ttcggaagga attgccagac aaggacatct ggtcctggac
    27241 cggctacact tgggaagaaa tgatgttgga aactccagat aaactggaat tcttgtcact
    27301 gattgacatt cttgtcgatg gaagatatga tcgaactaag agaaatctta tgctccagtt
    27361 tcgaggttca tctaaccaac gaattatcga tgtgcaaaaa tcgctcaaaa gtgggcaagt
    27421 agtgatttgg gacaagctca atgacggaaa agaaagctat gaacaggtga agagagaatg
    27481 aagaaaaagg acttagtaga ccaactagtc tcagagatcg agacggggaa agtcaggaca
    27541 ctgggaatat acggtcatgg agcttcaggt aaatcaacct ttgcacagga attgtaccaa
    27601 gctttagatt ctactacagt aaatttgcta gagacagatc cttatatcac ctcaggacgc
    27661 catctggtag tacccaagga cgcgccgaat caaaaggtga cagccagtct gccagtggcg
    27721 catgaactgg agagtttgca gagagatatc cttgccttgc aggcgggtat ggatgtcttg
    27781 acaattgaag aaccttggaa ggctagtgag gtcttgtctg gagccaaacc aattttgatt
    27841 gtcgaaggga tgtctgttgg ctttctaccc aaggaactct ttgaaaaaac catctgtttc
    27901 tacacggatg aggagaccga attaaagcga cgccttgcta gagatacgac tgtgagaaat
    27961 cgcgatgcat cctttatatt ggctagccat cagatgagac gggagcagta tctgcgatac
    28021 tataaagaaa ctgagtctaa ggcggatatc ttagtggacc aatcagaaga taaatttgat
    28081 gtcaagagga cttaacttat gtatagaaga aatatagaag aaaatcccta attttagaaa
    28141 gaaaaagtag gaaaacagtt tcatcccaaa aaaacgaaaa aacagcaaca aatcccttgc
    28201 aatcgcaggg gctttgtgtt attctattat ggtgctgtaa attacagcct tagttttgat
    28261 gcaagaggtt gcgacacgct cggttgcatt gccacgcaac accgcgtcgg ttttcttgtg
    28321 gagctagcct attatcttaa atagacgaaa aggagaaaaa gatggcaaac aaaaaaatcc
    28381 gtatccgttt gaaagcttac gaacaccgta cgcttgacac agcggctgca aaaatcgtag
    28441 aatcagctac tcgtacaggt gcacaagttg cgggtccaat cccacttcca actgaacgta
    28501 gcctctacac aatcattcgt gcgactcaca aatacaaaga ctctcgcgaa caatttgaaa
    28561 tgcgtacaca caaacgtttg atcgatatcg ttaacccaac tcaaaaaaca gttgatgcct
    28621 tgatgaaatt ggatcttcca agtggtgtaa acgtagaaat caaactttaa tctaaaatat
    28681 aaaagagcag aggctggtgt ttcaatctaa ttgaacacgg gctaaactcg gtgtgaaaaa
    28741 gataaacttc ctagtgtctg ctagacactg cgtcagtttc ctattttcac gttgagtttg
    28801 acgccctttg tatcttagac ttgagcataa aaaacgctcg ttaaaaactt tttgaataaa
    28861 aaatatagaa aaggaactat tttctcatga caaaaggaat cttagggaaa aaagtgggaa
    28921 tgactcaaat cttcactgaa gctggcgaat tgatccctgt aacagttatt gaagcaactc
    28981 caaacgttgt tcttcaagtt aaaactgttg aaacagacgg atacaacgct atccaagttg
    29041 gtttcgatga caaacgcgaa gtattgagca acaaacctgc taaaggacat gtagcgaaag
    29101 ctaacacggc tcctaagcgc ttcattcgtg aattcaaaaa cgttgaaggc ttggaagttg
    29161 gtgctgaaat tacagttgaa acattcgcag ctggagacgt tgttgacgta acgggtactt
    29221 ctaaaggtaa aggtttccaa ggtgttatca aacgccacgg acaatcacgt ggaccaatgg
    29281 ctcacggttc tcgttaccac cgtcgtccag gttctatggg gcctgttgca cctaaccgcg
    29341 tattcaaagg taaaaacctt gcaggacgta tgggtggcga ccgcgtaaca attcaaaacc
    29401 ttgaagttgt acaagttgtt ccagaaaaga acgttatcct tatcaaaggt aacgtaccag
    29461 gtgctaagaa atctcttatc actatcaaat cagcagttaa agctggtaaa taataaagaa
    29521 aggggaaatc agtcacaatg gcaaacgtaa cattatttga ccaaactggt aaagaagctg
    29581 gccaagttgt tcttagcgat gcagtatttg gtatcgaacc aaatgaatca gttgtgtttg
    29641 atgtaatcat cagccaacgc gcaagccttc gtcaaggaac acacgctgtt aaaaaccgct
    29701 ctgcagtatc aggtggtgga cgcaaaccat ggcgtcaaaa aggaactgga cgtgctcgtc
    29761 aaggttctat ccgctcacca caatggcgtg gtggtggtgt tgtcttcgga ccaactccac
    29821 gttcatacgg ctacaaactt ccacaaaaag ttcgtcgcct agctcttaaa tcagtttact
    29881 ctgaaaaagt tgctgaaaac aaattcgtag ctgtagacgc tctttcattt acagctccaa
    29941 aaactgctga atttgcaaaa gttcttgcag cattgagcat cgattctaaa gttcttgtta
    30001 tccttgaaga aggaaatgaa ttcgcagctc tttcagctcg taaccttcca aacgtgaaag
    30061 ttgcaactgc tacaactgca agtgttcttg acatcgcaaa tagcgacaaa cttcttgtca
    30121 cacaagcagc tatctctaaa atcgaggagg ttcttgcata atgaatttgt atgatgttat
    30181 caaaaaacct gtcatcactg aaagctcaat ggctcaactt gaagcaggaa aatatgtatt
    30241 tgaagttgac actcgtgcac acaaactttt gatcaagcaa gctgttgaag ctgctttcga
    30301 aggtgttaaa gttgccaatg ttaacacaat caacgtaaaa ccaaaagcta aacgtgttgg
    30361 acgttacact ggttttacta acaaaactaa aaaagctatc atcacactta cagctgattc
    30421 taaagcaatc gagttgtttg ctgctgaagc tgaataatct aaggaggaaa tatcgtggga
    30481 attcgtgttt ataaaccaac aacaaacggt cgccgtaata tgacttcttt ggatttcgct
    30541 gaaatcacaa caagcactcc tgaaaaatca ttgcttgttg cattgaagag caaggctggt
    30601 cgtaacaaca acggtcgtat cacagttcgt caccaaggtg gtggacacaa acgtttctac
    30661 cgtttggttg acttcaaacg taataaagac aacgttgaag cagttgttaa aacaatcgag
    30721 tacgatccaa accgttctgc aaacatcgct cttgtacact acactgacgg tgtgaaagca
    30781 tacatcatcg ctccaaaagg tcttgaagta ggtcaacgta tcgtttcagg tccagaagca
    30841 gatatcaaag tcggaaacgc tcttccactt gctaacatcc cagttggtac tttgattcac
    30901 aacatcgagt tgaaaccagg tcgtggtggt gaattggtac gtgctgctgg tgcatctgct
    30961 caagtattgg gttctgaagg taaatatgtt cttgttcgtc ttcaatcagg tgaagttcgt
    31021 atgattcttg gaacttgccg tgctacagtt ggtgttgtcg gaaacgaaca acatggactt
    31081 gtaaaccttg gtaaagcagg acgtagccgt tggaaaggta tccgcccaac agttcgtggt
    31141 tctgtaatga accctaacga tcacccacac ggtggtggtg aaggtaaagc accagttggt
    31201 cgtaaagcac catctactcc atggggcaaa cctgctcttg gtcttaaaac tcgtaacaag
    31261 aaagcgaaat ctgacaaact tatcgttcgt cgtcgcaacg agaaataata ttaaactagt
    31321 cgcttaagca actagtaaat ccgccagctc ggtagcgctc cataggagtg caagccgctg
    31381 tggtacaaca tttaaaggag aaaatataaa aatgggacgc agtcttaaaa aaggaccttt
    31441 cgtcgatgag catttgatga aaaaagttga agctcaagct aacgacgaaa agaaaaaagt
    31501 tattaaaact tggtcacgtc gttcaacgat cttcccaagt ttcattggtt acactattgc
    31561 agtttatgac ggacgtaaac acgtacctgt ttacatccaa gaagacatgg taggccacaa
    31621 acttggtgaa tttgcaccaa ctcgtactta caaaggtcac gctgcagacg acaagaaaac
    31681 acgtagaaaa taaggagaac ataaatggca gaaattactt cagctaaagc aatggctcgt
    31741 acagtacgtg tttcacctcg taaatcacgt cttgttcttg ataacatccg tggtaaaagc
    31801 gtagccgatg caatcgcaac cttgacattc actccaaaca aagctgctga aatcatcttg
    31861 aaagttttga actcagctgt agctaacgct gaaaacaact ttggtttgga taaagctaac
    31921 ttggtagtat ctgaagcatt cgcaaacgaa ggaccaacta tgaaacgttt ccgtccacgt
    31981 gcgaaaggtt cagcttcacc aatcaacaaa cgtacagctc acatcactgt agctgttgca
    32041 gaaaaataag gaggtaaaat cgtgggtcaa aaagtacatc caattggtat gcgtgtcggc
    32101 atcatccgtg attgggatgc caaatggtat gctgaaaaag aatacgcgga ttaccttcat
    32161 gaagatcttg caatccgtaa attcgttcaa aaagaacttg ctgacgcagc agtttcaact
    32221 attgaaatcg aacgcgcagt aaacaaagtt aacgtttcac ttcacactgc taaaccaggt
    32281 atggttatcg gtaaaggtgg tgctaacgtt gatgcactcc gtgcaaaact taacaaattg
    32341 actggaaaac aagtacacat caacatcatc gaaatcaaac aacctgattt ggatgctcac
    32401 cttgtaggtg aaggaattgc tcgtcaattg gagcaacgtg ttgctttccg tcgtgcacaa
    32461 aaacaagcaa tccaacgtgc aatgcgtgct ggagctaaag gaatcaaaac tcaagtatca
    32521 ggtcgtttga acggtgcaga tatcgcccgt gctgaaggat actctgaagg aactgttccg
    32581 cttcacacac ttcgtgcaga tatcgattac gcttgggaag aagcagatac tacatacggt
    32641 aaacttggtg ttaaagtatg gatctaccgt ggtgaagttc ttccagctcg taaaaacact
    32701 aaaggaggta aataaccaat gttagtacct aaacgtgtta aacaccgtcg tgagttccgt
    32761 ggaaaaatgc gcggtgaagc aaaaggtgga aaagaagtag cattcggtga atacggtctt
    32821 caagctacaa ctagccactg gatcactaac cgccaaatcg aagctgctcg tatcgccatg
    32881 actcgttaca tgaaacgtgg tggtaaagtt tggattaaaa tcttcccaca caaatcatac
    32941 actgctaaag ctatcggtgt gcgtatggga tctggtaaag gggcacctga aggttgggta
    33001 gcaccagtta aacgtggtaa agtgatgttc gaaatcgctg gtgtatctga agagattgca
    33061 cgtgaagcgc ttcgacttgc tagccacaaa ttgccagtta aatgtaaatt cgtaaaacgt
    33121 gaagcagaat aaggagaagg catgaaactt aatgaagtaa aagaatttgt taaagaactt
    33181 cgtggtcttt ctcaagaaga actcgcgaag cgcgaaaacg aattgaaaaa agaattgttt
    33241 gaacttcgtt tccaagctgc tactggtcaa ttggaacaaa cagctcgctt gaaagaagtt
    33301 aaaaaacaaa tcgctcgcat caaaacagtt caatctgaag cgaaataata gactagggaa
    33361 ggagaaattt caatggaacg caataatcgt aaagttcttg ttggacgtgt tgtatctgac
    33421 aaaatggaca agacaatcac agttgtagtt gaaacaaaac gtaaccaccc agtctatggt
    33481 aaacgtatta actactctaa aaaatacaaa gctcatgatg aaaacaatgt tgccaaagaa
    33541 ggcgatatcg tacgtatcat ggaaactcgc ccgctttcag ctacaaaacg tttccgtctt
    33601 gtagaagttg ttgaagaagc ggtcatcatc taatcaaacc tgaaaggaga aaactgaaat
    33661 gattcaaaca gaaactcgtt tgaaagtcgc agacaacagc ggtgctcgcg aaatcttgac
    33721 tatcaaagtt cttggtggtt caggacgtaa atttgcaaac atcggtgatg ttatcgtggc
    33781 atctgtaaaa caagctactc ctggtggtgc ggttaaaaaa ggtgacgttg ttaaagcagt
    33841 tatcgttcgt actaaatcag gtgctcgtcg tgctgatggt tcatacatca aatttgacga
    33901 aaacgcagca gttatcatcc gtgaagacaa aactcctcgc ggaacacgta tctttggccc
    33961 agttgcacgt gaattgcgtg aaggtggctt catgaagatc gtgtcacttg ctccagaagt
    34021 actttaattt ttaggaacaa actagtcccc tagcttcaag ctagggtgcc cttatgggcg
    34081 taagaaaaat caaggagaaa cctaatgttt gtaaaaaaag gcgacaaagt tcgcgtaatc
    34141 gctggtaaag ataagggaac agaagctgtt gtccttactg cccttccaaa agtaaacaaa
    34201 gttatcgttg aaggtgttaa cattgttaag aaacaccaac gtccaactaa cgagcttcct
    34261 caaggtggta tcatcgagaa agaagcagct atccacgtat caaacgttca agttttggac
    34321 aaaaatggtg tagctggtcg tgttggatac aaatttgtag acggtaaaaa agttcgctac
    34381 aacaaaaaat caggcgaagt gcttgattaa tcacgaagga aaggagaagt ataatggcaa
    34441 atcgtttaaa agaaaaatat cttaatgaag tagttcctgc tttgacagaa caattcaact
    34501 actcatcagt gatggctgtg cctaaagtag ataagattgt tttgaacatg ggtgttggtg
    34561 aagctgtatc aaacgctaaa agccttgaaa aagctgctga agaattggca cttatctcag
    34621 gtcaaaaacc acttatcact aaagctaaaa aatcaatcgc cggcttccgt cttcgtgaag
    34681 gtgttgcgat cggtgcaaaa gttacccttc gtggtgaacg tatgtacgaa ttcttggata
    34741 aattggtatc agtttcactt ccacgtgtac gtgacttcca cggtgtccca acaaaatcat
    34801 ttgatggacg cgggaactac acacttggtg tgaaagaaca attaatcttc ccagaaatca
    34861 acttcgatga cgttgacaaa actcgtggtc ttgacatcgt tatcgtaaca actgctaaca
    34921 ctgacgaaga gtcacgtgca ttgcttacag gccttggaat gccttttgca aaataatata
    34981 ggaggtaaat ctaatggcta aaaaatcaat ggtagctaga gaggctaaac gccaaaaaat
    35041 tgttgaccgt tatgctgaaa aacgtgctgc attaaaggcg gcaggggact acgaaggttt
    35101 atctaaatta cctcgcaacg cctcaccgac tcgtttacat aatcgttgta gggttacggg
    35161 gcgcccacat tcagtttacc gcaaatttgg tctgagtcgt atcgcttttc gcgaacttgc
    35221 gcataaaggt caaattcctg gtgtaacaaa agcatcttgg taatttaaga tatcaagagc
    35281 gtcaaaactc caagtaaaaa taggaaactt gacgaagaaa ctaaagtttc taggaaagtt
    35341 tatctttttc acacagagtt tagcccgggt tcaattgggc ttgccaattt gaacacgagc
    35401 tacagctttg gcaaaaaaga ccaatttgct ttggagcatt gcttctgcat taaattgtct
    35461 atttttgctc gtgctgttac gctctttgta tcatgtatta actagcaagt gcaacttgca
    35521 aactactagt aagaggagaa aaacaaaatg gttatgactg acccaatcgc agacttccta
    35581 actcgtattc gtaatgctaa ccaagctaaa cacgaagtac ttgaagtacc tgcatcaaac
    35641 atcaaaaaag ggattgctga aatccttaaa cgcgaaggtt ttgtaaaaaa cgttgaaatc
    35701 attgaagatg acaaacaagg cgtcatccgt gtatttctta aatacggacc aaatggtgag
    35761 aaagttatca ctaacttgaa acgtgtttct aaaccaggac ttcgtgtcta caaaaaacgt
    35821 gaagaccttc caaaagttct taacggactt ggaattgcca tcctttcaac ttctgaaggt
    35881 ttgcttactg ataaagaagc acgccaaaag aatgttggtg gtgaggttat cgcttacgtt
    35941 tggtaaaatc aagatacaaa gctcgtaaag aacaaagcaa aattaggaag ttggagaagt
    36001 ttgtttacaa acaagccaac ttatctattt tgcacagttc ttagagcgtg ttcagttcag
    36061 ctcttgaact aaataagtat ctgaaccccg tgaaaactgg ccgttctggc ctgacaattt
    36121 aacaggagaa aataaacatg tcacgtattg gtaataaagt tatcgtgttg cctgctggtg
    36181 ttgaactcgc taacaatgac aacgttgtaa ctgtaaaagg atctaaagga gaacttactc
    36241 gtgagttctc aaaagatatt gaaatccgtg tggaaggtac tgaaataact cttcaccgtc
    36301 caaacgattc aaaagaaatg aaaactatcc acggaactac tcgtgccctt ttgaacaaca
    36361 tggttgttgg tgtatcagaa ggattcaaga aagaacttga aatgcgtggg gttggttacc
    36421 gtgcacagct tcaaggatct aaacttgttt tggctgttgg taaatctcat ccagacgaag
    36481 ttgaagctcc agaaggaatt acttttgaac ttccaaaccc aacaacaatc gttgttagcg
    36541 gaatttcaaa agaagtagtt ggtcaaacag ctgcttacgt acgtagcctt cgttcaccag
    36601 aaccatataa aggtaaaggt atccgttacg ttggtgaatt cgttcgccgt aaagaaggta
    36661 aaacaggtaa ataatgttga gtggttgatc atcaaccacc aacctatttt ccaactttgt
    36721 gcatagcaca cgatttaaaa ctaaagaggt gaaaactgtg atttcaaaac cagataaaaa
    36781 caaactccgc caaaaacgcc accgtcgcgt tcgcggaaaa ctctctggaa ctgctgatcg
    36841 cccacgtttg aacgtattcc gttctaatac aggcatctac gctcaagtga ttgatgacgt
    36901 agcgggtgta acgctcgcaa gtgcttcaac tcttgataaa gaagtttcaa aaggaactaa
    36961 aactgaacaa gccgttgctg tcggtaaact cgttgcagaa cgtgcaaacg ctaaaggtat
    37021 ttcagaagtg gtgttcgacc gcggtggata tctatatcac ggacgtgtga aagctttggc
    37081 tgatgcagct cgtgaaaacg gattgaaatt ctaataggag gacactagaa aatggcattt
    37141 aaagacaatg cagttgaatt agaagaacgc gtagttgctg tcaaccgtgt tacaaaagtt
    37201 gttaaaggtg gacgtcgtct tcgtttcgca gctcttgttg ttgttggtga ccacaatggt
    37261 cgcgtaggat ttggtactgg taaagctcaa gaagttccag aagcaatccg taaagcagta
    37321 gatgatgcta agaaaaactt gatcgaagtt cctatggttg gaacaacaat cccacacgaa
    37381 gttctttcag aattcggtgg agctaaagta ttgttgaaac ctgctgtaga aggttctgga
    37441 gttgccgctg gtggtgcagt tcgtgccgtt gtggaattgg caggtgtggc agatattaca
    37501 tctaaatcac ttggttctaa cactccaatc aacattgttc gtgcaactgt tgaaggtttg
    37561 aaacaattga aacgcgctga agaaattgct gcccttcgtg gtatttcagt ttctgatttg
    37621 gcataagaaa ggggataaaa tggctcaaat taaaattact ttgactaagt ctccaatcgg
    37681 acgcattcca tcacaacgta aaactgttgt agcacttgga cttggcaaat tgaacagctc
    37741 tgttattaaa gaagataacg ctgctatccg tggtatgatc acagcagtat ctcacttagt
    37801 aacagttgaa gaagtaaact aatgaagttt taggggatgt gcactgtacc atcccctaaa
    37861 actagatata gtcatctatg atgacatcgt ataggcgagt tgatggggga gacaaccttt
    37921 tctcccttat cggcgctagc attttacaaa agaggagaaa ataaaaatga aacttcatga
    37981 attgaaacct gcagaaggtt ctcgtaaagt acgtaaccgc gttggtcgtg gtacttcatc
    38041 aggtaacggt aaaacatctg gtcgtggtca aaaaggtcaa aaagctcgta gcggtggcgg
    38101 agttcgcctt ggttttgaag gtggacaaac tccattgttc cgtcgtcttc caaaacgtgg
    38161 attcactaac atcaacgcta aagaatacgc aattgtgaac cttgaccaat tgaacgtctt
    38221 tgaagatggt gctgaagtaa ctccagttgt tcttatcgaa gcaggaattg ttaaagctga
    38281 aaagtcaggt attaaaattc ttggtaacgg tgagttgact aagaaattga ctgtgaaagc
    38341 agctaaattc tctaaatcag ctgaagaagc tatcactgct aaaggtggtt cagtagaagt
    38401 catctaagag aggtgaccta tgttttttaa attattaaga gaagctctta aagtcaagca
    38461 ggttcgatca aaaattttat ttacaatttt tatcgttttg gtctttcgta tcggaactag
    38521 cattacagtt cctggtgtga atgccaatag cttgaatgct ttaagtggat tatccttctt
    38581 aaacatgttg agcttggtgt cggggaatgc cctaaaaaac ttttcgattt ttgccctagg
    38641 agttagtccc tatatcaccg cttctattgt tgtccaactc ttgcaaatgg atattttacc
    38701 caagtttgta gagtggggta aacaagggga agtaggtcga agaaaattga atcaagctac
    38761 tcgttatatt gctctagttc tcgcttttgt gcaatctatc gggattacag ctggttttaa
    38821 taccttggct ggagctcaat tgattaaaac tgctttaact ccacaagttt ttctgacgat
    38881 tggtatcatc ttaacagctg gtagtatgat tgtcacttgg ttgggtgagc aaattacaga
    38941 taagggatac ggaaacggtg tttccatgat tatctttgcc gggattgttt cctcaattcc
    39001 agagatgatt cagggcatct atgtggacta ctttgtgaac gtcccaagta gccgtatcac
    39061 ttcatctatc attttcgtaa tcattttgat tattactgta ttgttgatta tttactttac
    39121 aacttatgtt caacaagcag aatacaaaat tccaatccaa tatactaagg ttgcacaagg
    39181 tgctccatct agctcttacc ttccgttaaa agtaaaccct gctggagtta tccctgttat
    39241 ctttgccagt tcgattactg cagcgcctgc ggctattctt cagtttttga gtgccacagg
    39301 tcatgattgg gcttgggtaa gggtagcaca agagatgttg gcaactactt ctccaactgg
    39361 tattgccatg tatgctttgt tgattattct ctttacattc ttctatacgt ttgtacagat
    39421 taatcctgaa aaagcagcag agagcctaca aaagagtggt gcctatatcc atggagttcg
    39481 tcctggtaaa ggtacagaag aatatatgtc taaacttctt cgtcgtcttg caactgttgg
    39541 ttccctcttc cttggtgtga tttccatttt accgattgca gctaaagatg tatttggtct
    39601 ttctgatgtt gttgcctttg gtggaacaag tctcttgatc attatctcta caggtatcga
    39661 aggaatcaag caattggaag gttacctatt gaaacgtaag tatgttggtt tcatggacag
    39721 aacagaataa aagtatttac tgaatcagta aatactgagg gagtggaggt ttaaactctg
    39781 acatttgtaa gagttggatc tcccctcttc tattttgttt ttaaatcggg gtgaaaagac
    39841 tttttgcttc tatttaaaaa taaaataagg agatcaaatc atgaatcttt tgattatggg
    39901 cttacctggt gcaggtaagg gaactcaagc agcaaaaatc gtagaacaat tccatgttgc
    39961 acatatctca acaggtgata tgttccgcgc tgcaatggca aatcaaactg aaatgggtgt
    40021 tcttgctaag tcatatattg acaagggtga attggttcct gacgaagtta caaatggaat
    40081 cgtaaaagaa cgcctttcac aagatgatat taaagaaaca ggattcttat tggatggtta
    40141 cccacgtaca attgaacaag ctcatgcctt ggacaaaaca ttggctgaac ttggcattga
    40201 actagaaggt gttatcaata ttgaagtgaa ccctgacagc cttttggaac gtttgagtgg
    40261 gcgtatcatc caccgcgtaa ctggagaaac tttccacaag gtctttaacc caccagttga
    40321 ctataaagaa gaagattact accaacgtga agatgataag cctgagacag taaaacgtcg
    40381 tttggatgtt aatattgctc aaggagaacc aatcattgct cactaccgtg ccaaaggttt
    40441 ggttcatgac atcgaaggta atcaagatat caatgatgtc ttctcagata ttgaaaaagt
    40501 attgacaaat ttgaaataaa gcgtttttca cacttgcaaa aatccgctac aaatgttata
    40561 ctgagatagt ctgacttata attgttgtct ctgtgtctag aggcatcgaa tcgaaattta
    40621 tggaggtgct tttgcgtggc aaaagacgat gtgattgaag ttgaaggcaa agtagttgat
    40681 acaatgccga atgcaatgtt tacggttgaa cttgaaaatg gacatcagat tttagcaaca
    40741 gtttctggta aaattcgtaa aaactatatt cgtattttag cgggagatcg tgttactgtc
    40801 gaaatgagtc catatgactt gacacgtgga cgtatcactt accgctttaa ataatcgaaa
    40861 aacttggagg gataagaaat gaaagtaaga ccatcggtca aaccaatttg cgaatactgt
    40921 aaagttattc gtcgtaatgg tcgtgttatg gtaatttgcc cagcaaatcc aaaacacaaa
    40981 caacgtcaag gataagatag aaaggagaaa acatggctcg tattgctgga gttgatattc
    41041 caaatgacaa acgcgtagta atctcattga cttatgttta tggtatcgga cttgcaacat
    41101 ctaagaaaat tttggctgct gctggaatct cagaagatgt tcgtgtacgt gatcttacat
    41161 cagatcaaga agatgctatc cgtcgtgaag tggatgcaat caaagttgaa ggtgaccttc
    41221 gtcgtgaagt aaacttgaac atcaaacgtt tgatggaaat cggttcatac cgtggtatcc
    41281 gtcaccgtcg tggacttcct gtccgtggac aaaacactaa aaacaacgcc cgcactcgta
    41341 aaggtaaagc tgttgcgatt gctggtaaga aaaaataata taggaggtaa aagtcttggc
    41401 taaaccaaca cgtaaacgtc gtgtgaaaaa gaatatcgaa tctggtattg ctcatattca
    41461 cgctacattt aataacacta ttgttatgat tactgatgtg catggtaatg caattgcttg
    41521 gtcatcagct ggtgctcttg gtttcaaagg ttctcgtaaa tctacaccat tcgctgctca
    41581 aatggcttct gaagctgctg ctaaatctgc acaagaacac ggtcttaaat cagttgaagt
    41641 tactgtaaaa ggtccaggtt ctggtcgtga gtcagctatt cgtgcgcttg ctgccgctgg
    41701 tcttgaagta acagcaattc gtgatgtgac tccagtgcca cacaatggtg ctcgtcctcc
    41761 aaaacgtcgc cgtgtataat catcgcatta cactgctttt cgtttaagag ggagtaacta
    41821 aatgatcgag tttgaaaaac caaatataac aaaaattgat gaaaataaag attatggcaa
    41881 gtttgtaatc gaaccacttg aacgtggcta cggtacaact cttggtaact ctcttcgtcg
    41941 tgtacttcta gcttctctac caggagcagc tgtgacatct atcaacattg atggtgtgtt
    42001 acatgagttt gacacagttc caggtgttcg tgaagacgtg atgcaaatca ttctgaacat
    42061 taaaggaatt gcagtgaaat cgtacgttga agacgaaaaa atcatcgaac tggatgttga
    42121 aggtcctgct gaagtaacag ctggtgacat tttgacagat agcgatattg aaattgtaaa
    42181 tccagatcat tatctcttta caatcggtga aggttcttct ctaaaagcga ctatgactgt
    42241 taacagtggt cgtggatatg tacctgctga tgaaaataaa aaggataatg caccagttgg
    42301 aacacttgct gtagattcta tttatacacc agttacaaaa gtcaactatc aagtggaacc
    42361 tgctcgtgta ggtagcaatg atggtttcga caaattaacc cttgaaatct tgacaaatgg
    42421 aacaattatt ccagaagatg ctttagggct ttcagcacgt attttgacag aacatcttga
    42481 tttgtttaca aatcttactg agattgctaa gtcaactgaa gtgatgaaag aagctgatac
    42541 tgaatctgac gaccgtattt tagatcgtac gattgaggaa ctggacttgt ctgtgcgttc
    42601 atacaactgt ttaaaacgtg ccggtatcaa tactgtgcat gatttgacag aaaaatctga
    42661 agcagagatg atgaaagtac gaaatcttgg acgcaagagt ttggaagaag tgaaactcaa
    42721 actcattgat ttgggtcttg gattaaaaga taaataaagg aggaatacat ggcttaccgt
    42781 aaactaggac gcactagctc acaacgtaaa gcaatgcttc gcgatttgac aactgacctt
    42841 ttgatcaacg aatcaatcgt gacaactgaa gctcgtgcta aagaaatccg taaaactgtt
    42901 gaaaaaatga ttactctagg taaacgtggt gatttgcatg cacgtcgtca agcagctgct
    42961 ttcgtacgta atgaaatcgc atctgaaaac tatgatgaag caactgataa gtacacttct
    43021 actacagcac ttcaaaaatt gttctcagaa atcgcacctc gttatgctga acgtaacggt
    43081 ggatacactc gtatccttaa aactgaatca cgtcgtggtg atgcagcgcc aatggcgatc
    43141 atcgaattag tataaaatca tcaattttgt tgagtgttat gatgatggag tcttgtgctc
    43201 ttagtctagc tctggtctac cgctaggatt tcggtcctag cgggaacact catcataagt
    43261 tgggatagta gacgcttgtt tacgaaattg tttttttctt aagaacaact tcgtaagcag
    43321 gcgtttttga gtattttcgt tagaattatg ctatactatt tgaaaagaat cctgtttaat
    43381 gttaaggttt cttattttaa gaagaattgg agtttactta tgaaagccat tataactgtt
    43441 gttggtaaag ataaatctgg aattgttgca ggtgtttctg gtaaaattgc agaattagga
    43501 ttgaatattg acgatatctc tcaaactgtc ttggatgaat attttacgat gatggctgtt
    43561 gtatctagtg atgaaaagca agattttacc tatcttcgta atgaatttga agcttttggg
    43621 caaactttga atgtaaaaat caatattcag agtgcagcga ttttcgaagc tatgtataat
    43681 atctaggagg tcatcatgga tattagacaa gttactgaaa ccatcgccat gattgaggag
    43741 caaaacttcg atattagaac cattaccatg gggatttctc ttttggactg tatcgatcca
    43801 gatatcaatc gtgctgcgga gaaaatctat caaaaaatta cgacaaaggc ggctaattta
    43861 gtagctgttg gtgatgaaat tgcggctgag ttgggaattc ctatcgttaa taagcgtgta
    43921 tcggtgacac ctatttctct gattggggca gcgacagatg cgacggacta cgtggttctg
    43981 gcaaaagcgc ttgataaggc tgcgaaagag attggtgtgg actttattgg tggtttttct
    44041 gccttagtac aaaaaggtta tcaaaaggga gatgagattc tcatcaattc cattcctcgc
    44101 gctttggctg agacggataa ggtctgctcg tcagtcaata tcggctcaac caagtctggt
    44161 attaatatga cggctgtggc agatatggga cgaattatca aggaaacagc aaatctttca
    44221 gatatgggag tggccaagtt ggttgtattc gctaatgctg ttgaggacaa tccatttatg
    44281 gcgggtgcct ttcatggtgt tggggaagca gatgttatca tcaatgtcgg agtttctggt
    44341 cctggtgttg tgaaacgtgc tttggaaaaa gttcgtggac agagctttga tgtagtagcc
    44401 gaaacagtta agaaaactgc ctttaaaatc actcgtatcg gtcaattggt tggtcaaatg
    44461 gccagtgaga gactgggtgt ggagtttggt attgtggact tgagtttggc accaacccct
    44521 gcggttggag actctgtggc acgtgtcctt gaggaaatgg ggctagaaac agttggcacg
    44581 catggaacga cggctgcctt ggccctcttg aacgaccaag ttaaaaaggg tggagtgatg
    44641 gcctgcaacc aagtcggtgg tttatctggt gcctttatcc ctgtttctga ggatgaagga
    44701 atgattgctg cagtgcaaaa tggctctctt aatttagaaa aactagaagc tatgacggct
    44761 atctgttctg ttggattgga tatgattgcc atcccagaag atacgcctgc tgaaactatt
    44821 gcggctatga ttgcggatga agcagcaatc ggtgttatca acatgaaaac aacagctgtt
    44881 cgtatcattc ccaaaggaaa agaaggcgat atgattgagt ttggtggtct attaggaact
    44941 gcacccgtta tgaaggttaa tggggcttcg tctgtcgact tcatctctcg cggtggacaa
    45001 atcccagcac caattcatag ttttaaaaat taagaaaata ggagaaattt taagttctat
    45061 ttaagattag acgtgtatac tataatcatt aaataaagac ctcctaatat tatttgaaac
    45121 agataacact gaattagttt gaatttgatt ttcatctaat atctttattt aatgaactcc
    45181 taaacttttt cataataatc tccttcaaaa gtcgcctgta tgggtggctt ttattttatc
    45241 attcatgata taatagaagc aaacggagga cggaaaatgg taaaagtacg attgtatttg
    45301 gtacgtcatg gcaagaccat gtttaacacg attggtcgcg cgcaaggttg gagcgatact
    45361 cccttaactg ctgaaggtga acgagggatt caagagttag gaatcggttt gcgagaatct
    45421 gatctacagt ttgagcgtgc ttattcgagt gattctggtc gtaccattca gaccatggga
    45481 attatccttg aagaacttgg cttgcagggg gaaatccctt atcgcatgga caagcgtatc
    45541 agagaatggt gtttcggtag ttttgatgga gcctatgatg gcgatctttt catgggcatt
    45601 attcctcgta tctttaatgt ggaccacgtt caccaattgt cttatgctga actggctgag
    45661 ggcttggtag aggtcgatac agctggttgg gctgaaggct gggaaaaact cagtggccga
    45721 atcaaggaag gctttgaaat gattgcaaaa gaaatggaag atcaaggtgg aggtaacgcc
    45781 cttgttgtca gccatggaat gactattgga accattgttt atctgattaa tggcatgcat
    45841 ccgcatggtc tggataatgg tagcgtgaca atccttgaat atgaggacgg ccagtttagg
    45901 gttgaagttg tcggtgaccg tagttaccga gagctaggac gtgagaagat ggaagaaggc
    45961 tctatttaat cagtctagac ttgcttgcca tgagctaggg atttgataag aatatcaaga
    46021 taagaaaaaa cagccgaggg cactcctttc ggctgttttt gatgtggaaa actaaagtgt
    46081 aatgctattg cttttagaga ttttcataaa caagagcaag gaacctactg ttagaacagt
    46141 caggatagtt gacaaggttg cggctacacc gtaatttcct ctgagaacct ctgtataaat
    46201 agctacagtc attgttcttg ttttgacatt gtagaggagg atagaagtag agagttttga
    46261 aatcattgtg actcaagata agatggctcc agaaatgata ccagatagca tcattggagt
    46321 tgtaatctta gcaaaggtat tgagacgact acttcctaag ctttcagcag cttcttcaat
    46381 acttggtgct atttgttgta agctagcaac agatgagcga atagtataag gtaatcttct
    46441 ggcagataga gacataatca agatgaaagc agtccctgta atcataagaa atccacttcc
    46501 aaatagacca gtattgaagg aagaaatgaa ggcaatccct agaacggttc ctggtacaat
    46561 ataaggtacc atactgaggc tgtcaattaa gtttgtaaac aaattccgtt ttctaacggc
    46621 taggtaggag ataaatgtcg caaatagaac aactagaact aaggcaatca aagggatacg
    46681 aatggtattg aaaatagcag atcccatacg atggaaagct accttgtaac tgtttggaga
    46741 ataaccttta acagatacca tacctgatgt ttttaggaaa gaggtataaa ttaagtagat
    46801 ttgaggtaaa acagagataa agataattcc gtagactgtt gcataaatgg cagccatttt
    46861 tccttttgta gtttttttag gctcaattgg atggagcaga ttcatgctga aactgtagcg
    46921 gtttgcaatg tgtttttgga taaggaaaat tgccaaggca atgataatcg ccataattgc
    46981 aaaagcagaa tttcctccaa cctcgctaat aaattgggta taaatcagga cagggaaagt
    47041 ccgataccct tcgccaatca acataggcgt tccaaagtct gagaatgctc tcataaatac
    47101 aagcaaggag ctgctagtaa ggttggaact aggagaggta aaacaaccgt tacgataggt
    47161 ttaaatccga aggaccccat gctttcagct gcttcaagta gagaattgtc aatactgttc
    47221 attgttccag caacatatag aaataccagt gggaatagtt gcagtgtaaa gacaagtaca
    47281 attcctttga atcaataaat atcgatagct ggaagataaa gggcatttgt caaaaattta
    47341 gtgatgacct catttcgtcc tagcaagaga acccaggagt aggctcctac gaaaggagct
    47401 gacatggaag caatgataat caatatttgt agaaatttct tccccttgaa gtcatacata
    47461 gagaagagat aagctaatag ggttcctaca actaaggaag tgatagtagc ggtaatggaa
    47521 accttgaaac tgttgactag tgtctcagag tagtaggctt tactaaagaa agtgacaaaa
    47581 ttagctagtg agaattgtcc ttcatgtata agtgcttgct tgagcacggt aacgatagga
    47641 taaacgagaa agataggata ggtaagaaag aggaagaaag aggaaactgt ccaaatattt
    47701 agttttttac gttccatggt tgactccttt tatcaggttt tgggaaccat ctgcagaaaa
    47761 gatgtttaat ttttgcgtat tgattcgtag acgaatacga ttgccttttt gtagatcttc
    47821 ttcaaaagtt gattcttcac taacttgaat ttttgaggca aaacctgtct caatgaaata
    47881 atccgtattt agtccaagat agacgctatc tctaatagtt ccttcaatat ctccagattc
    47941 atctttgata aactcttcgg gacgaatgct tacatgaata gcttgctcct caacctgatc
    48001 aagagctggc attcgaaggg catagccatc tgaaaagacg atataagcgc cgtcgctccg
    48061 tttttcaaga ttggcaggga taatatttgt gcgtccgata aaggttgcca caaactcatt
    48121 agctggttta tgatagagtt cttttggtcg gccgatttgt tggatcaccc catctttcat
    48181 aacagcaatt tggtctgaaa tagccatggc ttcttcttgg tcgtgggtta cataaacagt
    48241 tgtaattccc acttcgtgtt ggatttctcg gatggcttga cgcatatcca agcgaagttt
    48301 ggcctccaga ttactaagtg gctcgtccat gaggagaaca cttggattaa ccgctaaggc
    48361 gcatgccaag gtgacacgtt gttgttgtcc accactgagt ttatcgggct ttcgatccgc
    48421 atattgagca atttgcatga gttcaagata cttgttggtc tgttgaatca attcttcttt
    48481 tggaaccttc ttttgcataa gaccaaaagc aacgttgtct cggacagtca aatgtgggaa
    48541 aatagcgtag ttttggaaaa ccatcccgat attgcgtttg ctgggttcca tattattgat
    48601 ttttgtatca tcgaagtaaa attctccacc ttcgatactg ttgaaacctg caatcatacg
    48661 aagaagggtc gttttcccac atcctgaagc tccaagaagg gtaaagagac ttccttttgg
    48721 aattgtaatg ttcaaattct caataacagg gacatcgtgg tagatttttt tggcgttaat
    48781 aattttgatc tcactcatag tgaacctctt ttactgttta gattggatat ctgtaaagac
    48841 ttcgttgtat ttcttaacga tatctgattt attcttgatg acataatcat aatcttcagt
    48901 gagtgttttg attttgtcaa ttggtttcat gttttcgctt gttttagcat ttttacgaac
    48961 aggacggtta gtagtggttg taccaagtgt atcttgtact tcttgagaga taataaaatc
    49021 gataaatttc ttggcatttt ccatattttt agatttttta acgatagcag cactagcagg
    49081 taggaagacg gttccttctt ttggatagac taccttaatg ttagctccgt catttaagag
    49141 tttaactgct ggatcttcat aagagagacc aacagccatt tctccatcag cgactacttt
    49201 atagacacta gatgaacttg aaccgatttt accatcaata agtgtgaaaa gatcttttac
    49261 ataagaccaa gccttatcat ctttgtaacc accttgagct tgtagcatat ttgttaattg
    49321 agcaaaggtg ctagaagagt ttgctgggtc agcagttgcg atttttcctt ttagttcagg
    49381 tttgaaaaga tcgttatatc cttcgatgtt catgccttta gttaaatcag ggttgacgat
    49441 taaaacacta ccatctagtg tataaggagt agagtagcca gttgtgtttt gatattcttt
    49501 gataacatta tcattttctt ttgaagtata gttttcaaag agttctccgt gggtagtata
    49561 ttgtgtataa gaaccaccaa agataacatc agctacagga acttcttttt ctgactctag
    49621 ttttttgaaa agttctccag taccagcttg aatcagttct actttgatac catatttttc
    49681 ttcaaaggca ggaatagttg ctccaattaa gccctctgag tttggtgaat aaacgactag
    49741 cgaaccgccg tctcctttat cagatgaact gtcatcggca gattcattag aagaacaagc
    49801 agcataatac atccatttct ttttcatgat ggatacctcc gttgtgttat ttaagtttat
    49861 tttaaaacaa tgtaagcgtt tttaaaacat acaattctat tctatagtgt attgaatcta
    49921 taacagtaca ctttgactgc taaaatattt ctataaatta atttgacttt cctgatagag
    49981 atgttcacat cttatttcaa ttcactatat tagagtaaaa ttctctacaa aaagaagaat
    50041 agcctatttt actattcttc tgagtgattt caattccttt ggggaaatat ggagatactt
    50101 tttaaatcct gacaaatggt tgtttctttt tctaaatcgg tgatactgta tcggagaatg
    50161 cgcgtgaggt cacaaaggct gcgatagagc ttctatggag aatttctttt tggagagatt
    50221 ttttaaagga atgagacatc cgctacctcc ttggaaggtt tttttgtgat aagtatagca
    50281 gtttaaaaat tctagagcaa ggtaaaatag aaaaggtttg gctaaaattt tttagctaaa
    50341 ccttttgaaa agttaagtat gtattataga tgcttgttat ctgcaaatag ctagagcatt
    50401 tcaaggaaat aaactagaaa taacaattaa tatggtggtt cagaaatacc ttttgataat
    50461 caataagat
//
LOCUS       DAVHEW010000004        47877 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVHEW010000004 DAVHEW010000000
VERSION     DAVHEW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47877
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.004"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VVL53_000239"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VVL53_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU4182462.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VVL53_000240"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VVL53_000240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU4182463.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VVL53_000241"
     CDS             complement(4065..4628)
                     /locus_tag="VVL53_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4182464.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VVL53_000242"
     CDS             complement(4713..5267)
                     /locus_tag="VVL53_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU4182465.1"
                     /translation="MVKVAVILAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VVL53_000243"
     CDS             complement(5278..6501)
                     /locus_tag="VVL53_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU4182466.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VVL53_000244"
     CDS             complement(6538..8988)
                     /locus_tag="VVL53_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU4182467.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9188..10117)
                     /locus_tag="VVL53_000245"
     CDS             complement(9188..10117)
                     /locus_tag="VVL53_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU4182468.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLNFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10257..10544
                     /locus_tag="VVL53_000246"
     CDS             10257..10544
                     /locus_tag="VVL53_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU4182469.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10578..11912)
                     /gene="ciaH"
                     /locus_tag="VVL53_000247"
     CDS             complement(10578..11912)
                     /gene="ciaH"
                     /locus_tag="VVL53_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU4182470.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11902..12576)
                     /gene="ciaR"
                     /locus_tag="VVL53_000248"
     CDS             complement(11902..12576)
                     /gene="ciaR"
                     /locus_tag="VVL53_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU4182471.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12685..15231)
                     /locus_tag="VVL53_000249"
     CDS             complement(12685..15231)
                     /locus_tag="VVL53_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4182472.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLAAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VVL53_000250"
     CDS             complement(15354..15794)
                     /locus_tag="VVL53_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU4182473.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VVL53_000251"
     CDS             complement(15769..16638)
                     /locus_tag="VVL53_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU4182474.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNILLQS
                     CQEFVKDYLDTICSFYLGKEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16616..17116)
                     /locus_tag="VVL53_000252"
     CDS             complement(16616..17116)
                     /locus_tag="VVL53_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4182475.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17127..17825)
                     /locus_tag="VVL53_000253"
     CDS             complement(17127..17825)
                     /locus_tag="VVL53_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU4182476.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17818..18084)
                     /locus_tag="VVL53_000254"
     CDS             complement(17818..18084)
                     /locus_tag="VVL53_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU4182477.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18150..19076)
                     /locus_tag="VVL53_000255"
     CDS             complement(18150..19076)
                     /locus_tag="VVL53_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU4182478.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19151..19966)
                     /locus_tag="VVL53_000256"
     CDS             complement(19151..19966)
                     /locus_tag="VVL53_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182479.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(19970..20290)
                     /locus_tag="VVL53_000257"
     CDS             complement(19970..20290)
                     /locus_tag="VVL53_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU4182480.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20632..20712)
                     /locus_tag="VVL53_000258"
                     /pseudo
     CDS             complement(20632..20712)
                     /locus_tag="VVL53_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20756..22753)
                     /gene="metG"
                     /locus_tag="VVL53_000259"
     CDS             complement(20756..22753)
                     /gene="metG"
                     /locus_tag="VVL53_000259"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU4182481.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22877..24136)
                     /locus_tag="VVL53_000260"
     CDS             complement(22877..24136)
                     /locus_tag="VVL53_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4182482.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24138..24839)
                     /locus_tag="VVL53_000261"
     CDS             complement(24138..24839)
                     /locus_tag="VVL53_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182483.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24823..26022)
                     /locus_tag="VVL53_000262"
     CDS             complement(24823..26022)
                     /locus_tag="VVL53_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4182484.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26287..27633
                     /gene="gor"
                     /locus_tag="VVL53_000263"
     CDS             26287..27633
                     /gene="gor"
                     /locus_tag="VVL53_000263"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU4182485.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27830..28366
                     /locus_tag="VVL53_000264"
     CDS             27830..28366
                     /locus_tag="VVL53_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU4182486.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            28417..28503
                     /locus_tag="VVL53_000265"
                     /pseudo
     CDS             28417..28503
                     /locus_tag="VVL53_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28572..28787)
                     /locus_tag="VVL53_000266"
     CDS             complement(28572..28787)
                     /locus_tag="VVL53_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU4182487.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28885..29205
                     /locus_tag="VVL53_000267"
     CDS             28885..29205
                     /locus_tag="VVL53_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182488.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29236..29574)
                     /locus_tag="VVL53_000268"
     CDS             complement(29236..29574)
                     /locus_tag="VVL53_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182489.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29586..30305)
                     /gene="trmD"
                     /locus_tag="VVL53_000269"
     CDS             complement(29586..30305)
                     /gene="trmD"
                     /locus_tag="VVL53_000269"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU4182490.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30295..30813)
                     /gene="rimM"
                     /locus_tag="VVL53_000270"
     CDS             complement(30295..30813)
                     /gene="rimM"
                     /locus_tag="VVL53_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU4182491.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELVLFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPSKRV
                     DVEILEGLDDED"
     gene            complement(30844..31632)
                     /locus_tag="VVL53_000271"
     CDS             complement(30844..31632)
                     /locus_tag="VVL53_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4182492.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
     gene            complement(32209..32448)
                     /locus_tag="VVL53_000272"
     CDS             complement(32209..32448)
                     /locus_tag="VVL53_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU4182493.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32468..32740)
                     /gene="rpsP"
                     /locus_tag="VVL53_000273"
     CDS             complement(32468..32740)
                     /gene="rpsP"
                     /locus_tag="VVL53_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU4182494.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32910..33119
                     /locus_tag="VVL53_000274"
     CDS             32910..33119
                     /locus_tag="VVL53_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182495.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33598..33811
                     /locus_tag="VVL53_000275"
                     /pseudo
     CDS             33598..33811
                     /locus_tag="VVL53_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33895..34698
                     /locus_tag="VVL53_000276"
     CDS             33895..34698
                     /locus_tag="VVL53_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4182496.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVIFHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34754..36295)
                     /locus_tag="VVL53_000277"
     CDS             complement(34754..36295)
                     /locus_tag="VVL53_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4182497.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36297..36779)
                     /locus_tag="VVL53_000278"
     CDS             complement(36297..36779)
                     /locus_tag="VVL53_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4182498.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(36779..37864)
                     /gene="rlmN"
                     /locus_tag="VVL53_000279"
     CDS             complement(36779..37864)
                     /gene="rlmN"
                     /locus_tag="VVL53_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU4182499.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKECVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(37885..38397)
                     /locus_tag="VVL53_000280"
     CDS             complement(37885..38397)
                     /locus_tag="VVL53_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU4182500.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDDK
                     GQRHFVIRQK"
     gene            complement(38553..39158)
                     /locus_tag="VVL53_000281"
     CDS             complement(38553..39158)
                     /locus_tag="VVL53_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU4182501.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39331..40368)
                     /locus_tag="VVL53_000282"
     CDS             complement(39331..40368)
                     /locus_tag="VVL53_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU4182502.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40402..41337)
                     /locus_tag="VVL53_000283"
     CDS             complement(40402..41337)
                     /locus_tag="VVL53_000283"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU4182503.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(41890..43080)
                     /locus_tag="VVL53_000284"
     CDS             complement(41890..43080)
                     /locus_tag="VVL53_000284"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU4182504.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43295..44638)
                     /locus_tag="VVL53_000285"
     CDS             complement(43295..44638)
                     /locus_tag="VVL53_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4182505.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44778..45593)
                     /locus_tag="VVL53_000286"
     CDS             complement(44778..45593)
                     /locus_tag="VVL53_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4182506.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(45645..47825)
                     /locus_tag="VVL53_000287"
     CDS             complement(45645..47825)
                     /locus_tag="VVL53_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU4182507.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT        14180 a        10487 c         8661 g        14549 t
ORIGIN      
        1 gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg aaatcacttt
       61 aatactaaat gaaaatcaaa aagcaaacta agagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaggtgg
      181 tggataaatc ggctgggcaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gtgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattaataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtaatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat ctgtaattga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaagatt gttgtttcag tgaagatttt tgggtccggt gtgaagtgaa cagttgttcc
     3541 tgttttatcc gtatctccaa ctatttcaag atctgcgaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccat ttttgtgaac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcgtgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttgaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaatata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttaatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcggaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tggaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactgt aatcgactct
     6481 tgagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagctcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgacgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca cagaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccatg accacttctt gttcctgttt tccccgagat tcaatcttga caaatttagc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccaag acttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagtcttct acttccaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttgccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggttacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 gtagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gcttccgttt tcttaaaata
     8341 tagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctggg tcatcttttt ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt ttgtaaaata
     9121 gtgaagaaat acgaaaaatt gagagaagaa atgtttgcat cttccttctc tcaacgaatc
     9181 atgtatttta tctaaccatg tcaggatcgt agtcataaaa tcctgtatca aggaaacggt
     9241 ttttagggtg aagtttacga acttcttcat ccagcaagaa ggcttgagca tgactaaagt
     9301 tgccctcttg aatcaagcta cgcgcttgga taaagagttt tgcaatctca acaaagttct
     9361 gaccaggagt gtagagccct tctagtttcc agcgagtaaa gccatgctcc accaattctg
     9421 ttaatttgat catcaaatca aggtcattgt tggcaaagat atgggtccca tgattatctt
     9481 caaaaatgga atagtggctc tctggatcac ttggctcagc caagaagagg tcacgtttat
     9541 gcgtcttttc atcatcgata tgtgtaaagt tatagtagtt ttgcaagagt ggacgtttag
     9601 aatgatggat gacgctagca ccgtaaacca aaacttcagc aggaatttcc aaaatctctg
     9661 gcattttgaa aagttcagct gatggaattt cacgcgccaa aacagcctca gatgcgccag
     9721 ccttttgtcc ccagaagtta atctgacgac tgctagttac catggttgaa gcatcgtaga
     9781 tggtcttaaa tgaataacca tcgcggttaa ctacgtaaaa gacgcctgca tccccaatcg
     9841 taatatagtc tgtcttgatt tcttccaaga agtttaagaa aggcttgata cggtccatca
     9901 tatcttggtg catgagagca ttgaccgcaa cgatcaattc cttaccagca tcatgaacca
     9961 acttagcgat ttcacgtaat tggtcataac taaaggtcgt tggcagacga agaccaaaat
    10021 ctttctcacc gacatagata cggtctacgc cagcttcgag tagttgttca acttgttcaa
    10081 tactttcagc agttgctgta atgacaatct tttccataag aaaaattata ccacatttct
    10141 ccaaaatcag ccatgctttg aaaatgaaaa gggactttat tctttttgta acctttctgt
    10201 aataattctc tgccgaaaaa atgataaaat aggtatgtac tgttaaggag agaatcatgc
    10261 ccgtaagaaa attacaatcc tatgaggtag actatcaaga agaattaaac cagcagcttc
    10321 ctcattacca agcttataca cctgaagcgc aatctgatgc caatctcaag gaaatcctat
    10381 tttttattaa tatcgctgtt ttttgtatct gtattgctat ctttagtttt atctttttag
    10441 cattaaaatt atcaactgct cttgcctttg ccgcagcaat cggattcagc ttacttgttt
    10501 taaaagttca acggtctatt atcaaacgaa aacgtagaag ataaatccaa aatatcgccc
    10561 caattggagc gatattttta ttttttcttt ttagatggtg tctgaatggc aatcttcact
    10621 tcaaagattg ttcccttggg tttattatct ttgacagtaa cagttccttt tagagcatct
    10681 acaatttgct tggctaggga taatcctaaa ccaaaaccac ctttttgccg ggttctagcc
    10741 ttgtctactc gataaaaacg gtcaaaaatt ttctttttat cttctgtcga aataccgatt
    10801 ccattatcag aaacaagtaa ataaagattg cgatcggtcg ccgagataag aaaatcaatt
    10861 tcaccatcct cctcagtata cttgacggca ttatcgaaaa gaatggtcat cagttgtttc
    10921 agaagaagct gatctgtgac aattgttcga tggatacgat tttcaaaacg gaagacacga
    10981 ttattttccg aagcaatcat ctcgtagttt gtgaaagttg tattaaaaaa gctagttgga
    11041 acttctgcaa gctccggctt aatcccatca tctctccgag ctaagttcag caagcttgtc
    11101 gttaaaaaac gcatatttcg gacttcttcc aaactcgatg caatgctttc gctcacatcc
    11161 ataatggtag cttctggctt acgaaaaagg gtctctaagc gattttgcaa aactgcgagt
    11221 ggagttcgta actcatgact ggcattttcc acaaaagact gttgcttctg catactctca
    11281 agcaggggcc taacactgac cctagctaga tagagactgg caagtaaaga caaaatccag
    11341 aaactagcca tcacgaccac aatcaattgc tcatgctttt gactagcctg ttccaactga
    11401 ctggtattaa tcaagacagc agcatacttg atattggttg aaaccgaact aatattggtc
    11461 tccatcaaaa tcacacgata gatttcttcc tgcccatagc tattaaaaac ctgaatctgg
    11521 tagatatgtc ctagttcttt cttttctaac ttaatcttat ccaagcccaa aaatcgattt
    11581 ccagaaagaa gttgagtaaa gtctttatca aagagaatga cttccgtatt ggaactgaca
    11641 ttaggtttta tttctacttt actagcgtcc gccctagcat tttctaaatc tttaatctct
    11701 tctgttgccc tatttatagc cagctgaata actgcttgag gattttcact cagtccatga
    11761 agcttatcgt ccaccgaagt ataaagactc gaatgcatga cttgtaaaat aatcagagtc
    11821 attgtagaaa aaatcagggt gaagacaccg aagttgcgga taaaataact aaagtcatcc
    11881 gcataccatg tttttttaag tttactgaac atcttttaaa agatacccaa cactacgcaa
    11941 agtttgcaaa ttctctgcaa aagtggttcc ctttaatttc ttacggactt ttgaaacata
    12001 gacttcgaca accgaaatcg ttgtatcact atcaaatccc catagacggt caaaaatctg
    12061 cgtcttaggc aaaatcacat tttgattttg aaggaaataa actagtaaat cgaactcttt
    12121 ccccagcaat tcgacaggag tatcttcaac tttaacggta ttggttgata aattaaccac
    12181 gatattccca taagtcaagg tgttttcatt aaacttccct gaacgtttga gaagggcctg
    12241 aatccgcatt ttaagttctt ctaggtagaa aggtttggtc agataatcat ccgctcccag
    12301 ttcaaatcca tgtcccttgt catccaaact ttccttggca gtcataatca gaactggtgt
    12361 cgtaattccc ttttcacgca attcttttaa gacttggaaa ccattttttt ctggcaacat
    12421 caaatccagc aaaatcaagt catagacacc actctcagct tcgtagagac cttcttctcc
    12481 atcaaatacc tgcataacat ccgcaaaatc gtctaaaaag tcaaatactg aatttgacag
    12541 acctaggtca tcctcaacca ataagatttt tatcatgaga aactcctcct tattaaaact
    12601 attataccaa atttgcctta aaaaaaactc aactctctgc attttacatg agatagctga
    12661 gttttctttt tattttaggc ttatttatgc atttccgtat tgaagaacaa ctgcttcgac
    12721 tgcagctttt tcacggctaa tcaagtcaac acgcgctgca atttccttga ttcccatacc
    12781 gatgttacgg ctaagagcaa ggtcagaaag ttgcggttca aagaactcct tgtattccgc
    12841 caagcgttgc tgagtcttaa atacatgagc aggaaggata acaaagctat caaagctcat
    12901 atctcctcca agggctgcct taatccaagc ccagttttca cgcgcccaag accaagctgt
    12961 tttctgagtt gcttgatgag ctaggaattg gtaataccaa gcagacaagt cctgtggttt
    13021 gaccacaaat ttgtccttcc aagaagtaat caggttttgg atattatccg catctgtact
    13081 gtatgcaaga gctgctgcca actggcgttt aaagacagca tctgttgcgt gagtataagt
    13141 atcaagataa agtgctaaca agtctttagt ctcatgatgt ttcatctcat taatcagaac
    13201 ttgtgagcga atagctgctg ggagtcctgc aagattctcc ttgtgtgttg cgaagatttg
    13261 gctagcgact tgactagctt ctgcatcatt tgagcgaatc atcatcgaaa cagccagctg
    13321 acgaaccaat tcatcctcat ctgattctcc gtctttagct tcaaaaccaa gacggtcata
    13381 gttatgacga gccaatttag caaccagtcc tttgaaggct gtttcagcat ccgttccttc
    13441 atcaataaag cgctcaaggg ctgaaatcac ttgagaaaca gctgaaacca ccagataaga
    13501 ctcttcctta gcaagtttat caagaactgg aagcaagtct gcataagaaa tgtgccctgc
    13561 ctcagccaac aaacgacgtt cttgaacaat ttgcagtttg cttgtgttat caagtgtctc
    13621 tagctcagca agaacagctg ctaacaagtc tccttgatag tcggtaatat agtgggcagt
    13681 attttcagtg ttgagacgaa gagctccttc attttcagca agaagagctg cgtagccagg
    13741 gatttcgata ctttcagttt cgagtgtatc aggcaagcct ttccagttgc tattgagggg
    13801 caccacccag agacggttct tgtcttcgtt ctcaccgatg aagaattgtt tttgtgaaat
    13861 cttcaagaca tcattttcaa ctttaacagt aagaactggg taaccaggct gttccaacca
    13921 agaatccatg aaggctgcga catcacgtcc tgacgcttga ccaagggcat cccaaaggtc
    13981 actaccaatg gtgttgctgt attggtgttt ttcaaagtag gcgtgcaaac ctttagcaaa
    14041 atcagcatct cctagccaac ggcgaagcat gtgcatgaga cggcttcctt tggcatagac
    14101 gatagcgccg tcaaagagtg tattgatttc atctggatgt ttaacttcga cgtggacaga
    14161 ctgaacgcca tcagtagcgt cacgttcaag agcaagaggt actccacctg tttggaaatc
    14221 ttcaaagata ttccagcttg gttcgatggt atccacacag acgtattcca tcatattagc
    14281 gaaactttca ttgagccaaa ggtcatccca ccatttcata gtcacgaggt tcccaaacca
    14341 ttggtgagcc aattcatggg ccacaacaag ggcaacttgt tgacggctag caaatgtaga
    14401 gttctcatcg acaaccaagt aaacttcacg gtaggtcaca agaccccagt tttccatagc
    14461 accagctgag aagtcaggaa gggcgatgtg gagagattga ggaattgggt acttaactcc
    14521 atagtaatct tcgtaaaact cgatagagcg aacagcgata tccagtgaga aatcaagatt
    14581 tgaaagtgga tgtgctttgg ttgagtagac acctaccagg gtaccatttt tagttttagc
    14641 ggtcacccct tgcaaatcac cagcaacaaa ggccaacaag taagaagaca tgcgaggtgt
    14701 tgtctcaaac ttccagatac ctgtttcctt acggttttca acatcgattt ctggcatgtt
    14761 tgacaaggcc aattcacctt ctgcttggtc aaagcgaaga gagaggtcaa aagttgcttt
    14821 ggcttcaggc tcatccacac atgggaaagc ttcgcgcgca aaatggctct cgaactgagt
    14881 agacaagacc tccttcttga ctccatcaac tgtataataa gaagggtaaa tccctgtcat
    14941 gttgtctgta attttaccag aaaaggcaag aaccaattca acttgaccag cctcagccaa
    15001 ttcgatatga agggcttcat tgtcatggtc aactgtaaat ggacgagctt gacctgcaac
    15061 ttctacagag gtgatttcca aatctttttg gtggagggag atgcggtcac tctgtgcttg
    15121 accagtgatg gtcactttcc cagaaaaagt cttggtctca cgactcaaat ctaaaaataa
    15181 atcataatgt tcaggaacaa attgcttaat aaaatgttca actgcttgca tagttttctc
    15241 ctattctaag tttaagagct gctgctcttc ttcaaacaaa cttattatat catattttgc
    15301 ttcagaaaaa aggattggac agcgatttcc aaaactttct tccttctaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcaatcct
    15421 gcttcaccca agcagtctct gaaaaaatcc tcatgaacct ggcgccaata gacaagtgtc
    15481 ttgtttccct caccttcctt ataggcatgg tcagctgaca ctcgattgaa gggtttaaca
    15541 gaaacctttg taatttcgac aatgcagaca gcctgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagcacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaggcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtgc tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatca atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccatatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaaac cctgctgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tagttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttcc ttacctaagt aaaaggagca aatagtatca agataatctt tcacaaattc
    16501 ctgacaactt tgtaatagta tattttgatt gagctctctc aataagtatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatca ttcaccttcc aattcatgta agaagtcttg tccagttctg
    16681 gaaatcctaa taattcagac ttaaccttca agactaatgg cgatgcattt tcttctgtaa
    16741 tctcttgaat atccatccaa atatatccaa gtgaatcatt cgcaccatca gacacagctt
    16801 ccgaaatcgt aacttgaggt gcactctcat tcatttcaac atcatacaag gctatgacat
    16861 ggtgaaccat aaaatttttt aactcttccc tgacgaaaac atcgtagatt cgaggattag
    16921 agtagcttct aacagtaaat cccgtctctt ccataacttc tctagtcagc gtttccgtca
    16981 gtccttcacc aagttgctga ctgcctccag gtagatcata ccgatgttga taagggcctc
    17041 tcgttttttc aatgcaaagt aactttccat tttcaaagca aacacagtag accccaaagt
    17101 gatttttgat ttccatccaa ctcctcctac ttcaaagacc agccaccatc tattgtcaag
    17161 atttgtcctt gcatggcgct cgcttttcca cttgctaaaa aaagactaag ctctgctatt
    17221 tcctctggct caatccagcg cttgattggg gtttcactag ccacccagtc agccaaacca
    17281 cctggttcaa aatccgcagc ggtcatagct gtcttgactg ctcctggagc gataccaaag
    17341 acctgaatcc cagcttcagc atagtctaga gccaactgct tggtgaagcc agccaaggca
    17401 tgcttggatg aagtataggc gtgaccacct ccacctgcta ggctagaagc aatggaacac
    17461 atattgatga tgattccctt tttattttcc agcatttgtg tcaaataata ccgagtcaac
    17521 tctactggaa taatgtagtt gatttcaaaa atctcttgaa tgtcctgcgc cgtttgttcc
    17581 aacagtggtt tgtaatcatc caaaactcca gcagtattac acaaaacatc cacctgaggg
    17641 caccagtcaa aaataggttc caagtccaag gtcaaatctc tctgtaaaaa gcgaaaatca
    17701 ccctctaaga gtggcttttc accttggtca actccataaa cttgatagcc cttctctaaa
    17761 aagaggcgag cttgagccaa tccgatccct gaactcactc ctgtaatgag tacacgttta
    17821 gtcatgcact tctacccaat ccgttgccaa aacatcacaa actgtcgggc tccacatgga
    17881 aaaaccttct ccttcgccag aaacgttgat taggaaataa ggtgtcattt caagtgcaag
    17941 cccattttgc tcgatggtat caaagagttg gacatagttt tccgcacctc cccaaccagt
    18001 tcgtacatat tttctcttag cctttaaccc aggcaggatc tcttcaaatg tcatgttttt
    18061 ctcctttaat tctacattct tcatttaatt atagcaaaaa accgctttat acggcttttt
    18121 gaatgtgagt tattcaaacc tgctactact tacggcaaat tattccctgc agcaagataa
    18181 atttcatacc attcttttct tgttaagcta aagtttgccg ctcggctaac ttctctcaag
    18241 tgcttaggat ttgttgtacc tacgactgcc tgcatttttg ctggataacg caatatccaa
    18301 gaaatggcaa tagttgaaga ggttactcca tatttaatag ctaaacgatc aagtacttga
    18361 tttaaagctt gaaatttctc atttccaaca aaattccctt taaaataccc gaattgtaag
    18421 acagaccatg cttgaatgac cacatcgtgt aattggcaat attcaaaaat gctgccatct
    18481 cgcatagctg cttgactatc ttccatatta acatgaaaag ctgattcaaa tcctggagta
    18541 aaagccgcac tcaattgtag ctgattaaca gctaacggct gcttgacatc ttttttaagc
    18601 aactccatca tcataggatt ttgattagaa actccaaaat ctcgaacttt accttgttta
    18661 taaaggagat taaaggcttc tgctacttgg tcagattcca tcaaagcatc tggtcgatga
    18721 aggagcaagc tatctagatg atcaatcttc aatctttgca aaataccgtc tactgatttt
    18781 ataatatagt ccttagaaaa atcaaaatag gtaaattctt caatgcgaat gccacatttg
    18841 gactgaatcc acatcttttc tcttaaatct ggacgatttt ttaggacaag acctaacagt
    18901 tcttcacaac gaccacgacc ataaatatca gccaagtcga aggcattgat tccaacagaa
    18961 agtgctgttt ctacaagctc ttcaacttct tttacagatt tatcttttat tctcatcatt
    19021 ccgagaacaa tttctgataa ttctttgtca tcttgaccaa gagttatgta tctcatcaaa
    19081 tttttctcct ttaatttcta acattcttcc cttcattata acaaaaaacc gctttgcaac
    19141 gactttttga ctatacttca ctccatttta tcttcttaaa cccacggaac aagacaaaga
    19201 ttccaataaa gaggacagct aaaggaataa cttttgtaag gaaaacattt gaaattccca
    19261 tccactcata ataacggagc agagaaccca ccacaagatg ggcaataatc atactgacaa
    19321 atggacgaaa gaccgcttct ttccaattcc aaataccgat aactagcgaa atcgtaaaga
    19381 cagacaaact atcccaggga gccggaatat aaaaggctcc ttcttgtatg aagcttgcca
    19441 ttcctacata tcctaaaaca actagaagaa ctatagtccc aacaacaatg taagtgccaa
    19501 ttttcatttt aggagaatct tggactaaac ttcttcgtaa aattgtggcc acaagtccaa
    19561 atccaatcag aaaaataaga agttgcccta aaaatgtgag caaattgact gttaagagag
    19621 gacctttaga aaaatcactt agtagttgat aataacgtaa taccgccagg acaagaattg
    19681 gcgtcaaaag ggactctttg atagaactgc gaggtgctcc cttgagaatc tctttcatta
    19741 tttttttagg attcttacct agataatcct ctgcactcat gccatctcgt tctgcttctg
    19801 agaaatctag catcatcaaa tagatctgct ctctgagata gtcttcatca tagagaaatc
    19861 cagcaagatt aaaactttcc cacaactcct caaaatactt ttgattctcc tcagaaaact
    19921 catgtagcaa agcgcttgtt tcttcgtaat acttcatttt cttcatggtt taacccccat
    19981 tcttaatccc ttctactttt tgactcaaat cgtcccattg ttgccaaaag actgagacac
    20041 gctcttctcc ttctttcatt aatgaaaaat acttccgatc tggaccatct ggcgacgggc
    20101 gcatgtcgcc tcttatccat tgattttttt ctaacttttg caacaaagga taaatagttc
    20161 ctggaacgat agtatcaaat ccagcctctc gcaaagtctg aaccaactca taaccatacc
    20221 gctctttttg accaatcata tccaagacac aaccttcaag aacacctttt aatagctgag
    20281 tttctttcat cacttctccc ttctaatcta ttttgtaata cctactagtg acttcaccta
    20341 tagtatatca cttctacact agtttgtaaa gcataatagt taatactctt cgaaaatctc
    20401 ttcaaaccac gtcagcgtcg ccctaccgta tgtatggtta ctgacttcgt cagtttcatc
    20461 tacaacctca aaaacatgtt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaaca
    20521 gtgttttgag ctgacttcgt cagtttcatc tacaacctca aaacagtgtt ttgagctgac
    20581 ttcgtcagtt tcatctacaa cctcaaaaac atgttttgag ctgacttcgt cagtttcgtc
    20641 tacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt gctctttgat
    20701 tttcattgag tataaataaa aaaacagaac tagcctgaac tagtcctgtc tacttttacc
    20761 caatcacact tccatttggt acagctggat caactgtgag aagggttaat ttgccatcat
    20821 gttcagctga gagaatcata ccctggctga catatttttt catcatttta cgtggtttga
    20881 ggttagcaat gatttgaact ttcttgccga ccaattcttg ttcatttgga tagtattttg
    20941 caattcctga aagaatctga cgatcttctc catcaccagc atccaagcgg aattgaagca
    21001 acttatctga accttctact ttagacactt ctttgacttc tgcgacacgg atttcaacct
    21061 tgtcaaagtc ttcaaacttg atttcatcct tgtttagttt gagctcaact tcgtccggat
    21121 tccattcttt ttcgactgct ggtttattgc cttccatttg ttccttgata taggcgattt
    21181 cttcttccat atttagacgt ggaaagatag gtgttccttt ggcaactaca gtcacatctg
    21241 ctgggaagtc agccaaactc aagttttcaa gactagaaac ttcttccaaa ccaagttgag
    21301 tcaaaactgc acgactagtt tccatcataa atggttcaat caagtgagca actacacgaa
    21361 tgctggctgc caagtggctc atgacacttg ccaattggtc acgaagagct tcatccttgg
    21421 ccaagaccca tggtgcagtc tcatcgatgt atttattggt acgagagatc agagtccaga
    21481 ctgcttcaag cgcacgtgga tagtcaactg cttccatgtg tgtatggaag tctgcgattg
    21541 attgttctgc aacctcagca agaacatgat caaattcagt cacaccttct acataggcag
    21601 ggatttgtcc atcaaagtac ttattaatca tggaaaccgt acggttaagg aggttcccaa
    21661 ggtcattagc caattcatag ttgatacggc cgacatagtc ttcaggagta aaggttccgt
    21721 ctgaaccaac tggaaggtta cgcatgaggt agtaacgaag tggatctagt ccataacgct
    21781 ctaccaacat ttcagggtaa acgacattcc cttttgactt agacattttt ccgtctttca
    21841 tgacaaacca accatgggca atcaaacgat caggtaattt aacatccaac atcataagaa
    21901 ggattggcca gtagatagag tggaagcgaa ggatatcttt tcctaccata tggaagactg
    21961 ttccattcca gaacttgtca aagttaccat gttcgtcttg agcgtagcca agagctgtcg
    22021 catagttaag aagggcatca atccaaacgt agacaacgtg ttttggattt gatgggacag
    22081 gcactcccca tgtaaaggtt gtacgagata ccgccaaatc ttccaaacct ggctcgatga
    22141 agttgcgtag catttcatta aggcgaccat ctggcgtgat aaattcagga tgagctttga
    22201 aaaattcgac caaacggtct tggtatttgc taaggcgaag gaagtatgat tcttcagaaa
    22261 cccattcaac ctcatgacct gatggagcaa taccaccagt cacatttcca gcttcatcac
    22321 ggaaaacttc tgccagctgg ctttctgtaa agaattcttc gtctgatact gaataccaac
    22381 cagagtattc acccaagtag atatcatctt gagcaagtaa gcgttcaaag acttgtgcga
    22441 caactttttc atggtagtca tcagttgtac ggataaattt atcgtatgag atatctagta
    22501 attgccagag ttctttaact ccaaccgcca ttccatcaac ataggcttga ggtgtaatac
    22561 cagcttcttc cgctttctgc tggattttct gaccatgttc atcaagacct gtcagataaa
    22621 atacatcgta gcccatcagg cgtttgtaac gtgctaggac atcacatgcg atagttgtgt
    22681 aggcagaacc gatatgaagt ttcccagatg gatagtaaat cggcgttgta atataaaaat
    22741 ttttttcaga cataattttt cctttccagg caaatgaaac ctgtttttct aacacttcat
    22801 tatatcacat ttttaatgaa tttcgatagg gaaatccata caaaaacaag atagacgagt
    22861 gtccatcttg ttgatctcat tcataacgaa gggcttcaat tggatcaagt ttcgatgcct
    22921 tgttggctgg caagactcca aaaatcatac caacactagc cgaaactgca agactaaata
    22981 gggcaactgg gattgatact ccaacttcta taccttctat taaaccttgc agtaacaaac
    23041 ctgctaaggc agttaaacca cttgcaattg tcaagccaat taagccacct aacaaggtca
    23101 aaatcatgga ttcaatcaaa aactgaatta aaatattggc acgtgttgcg cccaaagcct
    23161 tacgaagacc aatctcacga gtgcgctctg tcaccgaaac cagcatgatg ttcatgacac
    23221 cagttcctcc aacaaagaga gaaatccctg cgatggaact aataatcgtc gtcataaaac
    23281 taaacgattg ttgaatttct gcaaatacaa cggactcatc tgccacctgg tattctccct
    23341 gttgtaagcc tgcaagctct gtcatttttc gtgccagttc tggacccaga gttggggtta
    23401 aactggtatc attcactcga aagacaatac tagctatttc atctatatta aaattcgcag
    23461 caagggagat attggtagta ataggcaagc caccaaaccc atatattttt gaccttttag
    23521 cctccggact agtataaacc ccaatgaccc ggtaactaaa tccattgact tctacaacct
    23581 tgttaatagc ctcttgagga gattcaaata aactaatgga caattcctca tctagcaaaa
    23641 taacacttgc aaactctttg aaatcttgct ctctcagact acgacctgca ataatttcat
    23701 tcttaacagc gtccatgtaa gttctgtttc cacctgtcaa attagcattc tcaacctttt
    23761 tatcttgata ggtcaagatg gcattcgttg aattggttac atagtaacta tccactccct
    23821 tcagtttagc tgcctcttgg acccaggatt cttgcggttt tggcggttca acaggaattt
    23881 cctcttcctt tccagaaacc gtaaaagatg attgtttctg agtaaaagaa ccatctttac
    23941 tttttttagg agagaaaaag acgctaatat ttttctgaga tttagtcata tctttattga
    24001 cttgacgaga tagggaatca cccaaagcca taatcacaac aactgatgaa acaccgataa
    24061 taatcccaat catagtaagc aaagaacgca tcttgtgagc catgatagat gaaaaggcaa
    24121 atttcagatt ctgcatctta gttttcctcc tttcctaact gagcactgtc agacgaaatg
    24181 accccatccc gaatgacaat ctgacgttta gcataggcag caatctcagg ctcatgcgtt
    24241 accatgataa tggtttttcc ttctttattc aaatcaacca ataattgcat aatttggtta
    24301 cctgttttgg tatccaaggc tcctgtcggt tcatccgcta ggataataga aggattgttt
    24361 accaaggcac gcgcaatggc tacacgttgc ttttgaccac cagataattc tgaaggtaaa
    24421 tggtgactac gttctgtcaa ttcaaccttg tctaaatatt cctcagccaa cttgcgacgt
    24481 tttgaagacg aaactcctgc gtaaatcaag ggcaattcta cattttgcag agcattgagc
    24541 ttcgatagaa gaaagaactg ctgaaagaca aaaccgattt gttggttacg gaccttagcc
    24601 agttgttttt caccaagccc agccacttct tgaccttcaa gataatattc tccactggtt
    24661 ggtgtatcca acatgccaat cgtattcatc agagtggact taccagaccc agatggtccc
    24721 atgatggcta caaattcacc ctcattcact tctagattga tatttttgag aacctgcagt
    24781 tcttggtcac cattacggta gcttctgaag atatttttta gactaattag ttgcttcatc
    24841 agccttcacc tcttttcctt cttccaagga agatgttgga ttactgatga ccttagcacc
    24901 gttcgttaaa ccagaagtga tttcttgatt ttctgcgtca gcatttccca atgaaacctc
    24961 aactttttta gccttttgtt gttcatccac aatccagaca taatttttac tatcatccat
    25021 tactagactg ctaacaggaa caagaatagc cttagttttg cttttaacct caatgttgac
    25081 agaaaaacct tgtttcaaat caccaacctc gcctgtcaca tcaatagtat aagggtattt
    25141 agaacctgta ttattcccgg ctgctggact agctgcttca ccattgtttt taggatagtc
    25201 agaaatatag cttaatttcc cagtccattt tttatcagga tacactttag aagtaaagct
    25261 tacttcttga cctacagaaa ggttggctag attgtactca gacaattctc ccttgacttg
    25321 taaattttca ttgctgacaa tatgaaccat aacttgactc gcccctgttg gagatttaga
    25381 aacattgcta ttgacttcga ccacagttcc ctctagggta ctgagaacag ttgttgcatc
    25441 caattgactt tgagccttgc ttaattgcgc cgcagcatct gcacgcgcat cacgggcatc
    25501 acccaattga gcgtcaatag aagcaacaga atttccagcc actggagttg ggctttgcac
    25561 cgttgcatct tctcctccta ctggcgctgg taactgtgga gccggagctg aagcggcttc
    25621 atttcgtgct tgattgagtt cattgatatg acgatctgcc ctagctactg ctcgactagc
    25681 tgaatcatag gccgcctgcg cttctgaact actgtacttg actaaagcct gcccttcgct
    25741 gaccttatcg cccacagaaa caaggatttc atctaaatca cccttactag catcaaaata
    25801 aacatattgt tcattttttg ctgttactgt ccctgacaat aaaacagagg aggccacgct
    25861 tccttccttg gcaacaacaa gatgagtagg ctcatctttt agagcagtct gagaaggttg
    25921 tctaaagagt aaaatccccc cagcacccaa tacaactaca ctcgcagcac cgattgctgc
    25981 atacagttgc cactttttag ctttaccatt ctttttcttc ataatgaaac tccttttctt
    26041 ttttacaata ctttgctatt ataccaaatt tccctccagc aaacaataca gttcaggatt
    26101 aaacaatcgt tcggaatttt gcttttctat tgtactagat atataataca ctatcttgac
    26161 ttatttggca agcttttttg caattttttg tgcgtagcag atttttgcaa tcaaatcaaa
    26221 aaaagaataa aatataacta tcaaaaaaga cgctctacta tttaaaagat tacaaaggag
    26281 ttttcaatga gagaatatga tatcattgct atcggtggag gtagcggagg aatcgctacc
    26341 atgaaccgtg ctggtgaaca tggagccaaa gcagccgtta ttgaggaaaa gaaattaggt
    26401 ggaacctgtg tcaacgtcgg ttgtgttcct aaaaaaatca tgtggtacgg ggcgcaaatc
    26461 gctgagactt tccatcaatt tggagaagac tacggcttta agactactga tcttaacttt
    26521 gactttgcaa ccctacgtcg caatcgtgaa gcctacattg atcgcgctcg ttcttcttat
    26581 gatggtagtt ttaaacgcaa cggtgtagac ttgattgaag gtcatgctga atttgtagat
    26641 tctcatactg taagcgtaaa tggtgaactg attcgtgcta aacatatcgt gattgctaca
    26701 ggtgcccatc caagtattcc taatattcct ggtgctgagc taggtggctc ttctgatgat
    26761 gtatttgcct gggaagaact tccagagtca attgccattc taggcgctgg ttatatcgcc
    26821 gttgaattag ctggcgtact ccacactttt ggtgtcaaga cagatctctt tgttcgccgc
    26881 gatcgtcctt tacgtggttt tgattcctac atcgttgaag gtttggtcaa ggaaatggaa
    26941 agaacaaact taccacttca cactcacaaa gtccctgtca agttagaaaa aactactgac
    27001 ggcattacca ttcatttcga agatggtact agtcacacag ctagccaagt tatctgggct
    27061 acaggtcgcc gtccaaacgt taagggcttg caacttgaaa aagctggagt gactctgaac
    27121 gaacgtggct ttatccaagt ggatgaatac caaaatactg ttgttgaggg aatctatgct
    27181 ctaggtgatg taacgggcga gaaagaactg actccagttg caatcaaggc cggacgtacc
    27241 ctatctgaac gtctctttaa cggaaaaact actgcaaaaa tggattactc aactattcca
    27301 actgttgtct tttcacaccc tgctatcgga actgttggtt tgacagaaga gcaagctatt
    27361 aaagaatacg gtcaagacca aatcaaggtt tataaatcaa gctttgcatc tatgtactct
    27421 gcttgcactc gcaaccgtca agaatcccgt ttcaaactca taacagctgg ttcagaagaa
    27481 aaagttgtcg gacttcatgg aattggctac ggcgttgatg aaatgattca gggatttgcc
    27541 gttgctatca aaatgggagc aaccaaggct gactttgatg caactgtggc aattcaccca
    27601 actgcatctg aagaatttgt aaccatgcgt taatcgaaac aaaagaagct gatataaata
    27661 tgtctgcttc ttttttaatg attttcgaat caacaaaaaa attatccaac ttgactagtc
    27721 acgaaaatcg gctgttacag tttttgttac cttgttttta aaaaatggtt gacatcgact
    27781 cctatatgag tataatagtt actatacatt agaaaagagg ttaactattt tgaaaaaagc
    27841 tcacgtttat gctatccctg ctattggggc tgctctcatt gctgctttgg cacaaatcag
    27901 tcttccaatt ggacctgttc ccttcactct gcaaaacttt gcaatcggct tgattgctac
    27961 tgtctttaga ccgagagagg ctgtactttc tgctggactc tatcttcttc taggtgctat
    28021 cggtcttcct gtctttgcag gaggtggagc tggttttcag gctttagttg gccctactgc
    28081 aggctatctt tggttttatc tcgtttactc tggacttact tcctctctaa ccaacagcaa
    28141 gagtggtgtt gttaagattt ttcttgcaaa cctcttgggt gatgcccttg tctttgtcgg
    28201 cgggattctc agcttgcatt tcctagctgg aatggcattt gaaaaagctc ttgctgtggg
    28261 ggttcttccc tttatcattc cagaccttgg caaacttcta gctattagtt ttattagccg
    28321 tcccctactt caacgcctta aaaatcaggc ttactttact aactaaaaaa ggatatcgag
    28381 ttatcatgac tcaatatcct tttcttttat tttgaaaact tatactcaat gaaaatcaaa
    28441 gagcaaacta ggaagctagc cgcaggctgc tcaaaacact gttttgaggt tgtggatgaa
    28501 actgacgaag tcagtaacca tacatacggc aaggcaaagc tgacgcggtt tgaagagatt
    28561 ttcgaagagt attagttgcc tttaaaggca tccaccatcg tttgaaattc ttcatttgag
    28621 agagtaatcc ctttgcccat tttagtatgg tctggactcc aagcacgaat atcaaacttt
    28681 gcaggggcac cattaaagct cacacggtta atttccttgg tccaaccttt ttcgttttca
    28741 gaaagagtca acaagtgctc ttcgatttca aatgtaaatt ctgccatttt cttctccttt
    28801 tttagtttca ttagtttatt cgtaaaatct tgtagatttt aggaaaattt tatataatat
    28861 tgatataaaa gaagggaggc caatatgaga cataaattcc agcaagttct aaataaaata
    28921 catgattttt taaatggata tgaccaacct gaccagactg aaaccaactc ccttacagcc
    28981 actattgaag aggctatcca gaaacaaacc gctgttcacc ttatcttgtc tgagacaagc
    29041 tttacaggtg acatcatcaa atatgatcag caaggccagc aaattatcgt gaaaaatttt
    29101 tccaaaaatg tgagccggat tatccgtata agcgatattc aacgcctgcg atttgtcccc
    29161 tcaactgtcc aaacagccca aaaaaataga tttaagaaag agtgagatgt agttgcttca
    29221 tcccactctt ttttcttagc gaatttgttc aaaatgtaaa tgaactgcga tatgatctcc
    29281 ataaccactt ctttccaagt cacgttgtaa acgataggaa atgtagtgtt ctgcaatggt
    29341 aatgtaacct gcgcccaata aacgatgttc aaccatagat tgaatcatac tgatagtcgc
    29401 acgttccacc ttggcttctt gtaaatccaa aactaccttc ttagtgactt gagcaagatt
    29461 ttgacgcaaa tcatctgtca aaacataaac agtttgggct gccttcaaga tggcttggta
    29521 aatcttatct ggattaaatt cagcaatttc gccattacgt ttgattactt gcataggttt
    29581 ctcctttatt ctttgttttc tttgatttct gccagcattt tttcttcttc tactgtcagt
    29641 tgataatgtt caagtaaatc cggtctgcgc tcgtaggttt tctttaaact ctcgtacaat
    29701 cgccactgac gaatcttttc atggtggcca ctcatcaata catctggcac gaccatgcct
    29761 cgataatcat agggacgtgt gtactgagga tattctaaaa gacctgaaga aaaactatca
    29821 tcttggtggc tagactcctt gccaatcact tctggaatca ggcgaactgt agcatcaatc
    29881 atggtcatag ctgccaattc tccaccagtg aggacatagt cacctaggga aatctcatct
    29941 gttaccaagg tcttaatgcg ctcatcataa ccctcatagt gcccacagat aaagattagc
    30001 tcttcctctt gagccaaatc ttcagcataa gcctgatcaa actgctttcc agcaggatca
    30061 aggagaataa cgcgcggatt tttcttttca atagcatcaa aggaatcgaa aataggttgt
    30121 gctctgagca acatgccctg accgcctccg tagggctcat catctacatg acgggccttt
    30181 tcagcatttt ctcgaaaatt atgatactgg atatccaaga gccctttttc tcgagccttt
    30241 ccaacgattg agtgctccag tggagaaaac atctctggaa agagggttaa aatatcaatc
    30301 ttcatcgtct aacccttcta agatttccac atcgacccgt ttacttggaa tatcaacatt
    30361 gagaaccact ggtgggatat aaggtaaaag caaatcacgt ttgccttttc gtttgaccac
    30421 ccagacatca ttagcacctg gttgcaggat ttccttgatg gttccaacca agctatcacc
    30481 ctcatagact tccaaaccga taatctcgtg atagtaaaat tcaccatcgt ctaggtcatt
    30541 caaatcttcc tcagcgacct tgagactgta tcccttgtac ttttcgatag tattgatatg
    30601 gtacatatct ttgaatttaa taatgtcaaa gttcttctgt ttacggtggc tagcgatggt
    30661 cactgtttgg acaaactgat ctttttcatc aaacaaaacc agctcagctc cttttttaaa
    30721 ccgttcttct gcaaaatccg tcacagacaa gactcgcatc tccccctgta atccctgcgt
    30781 attaacgatt ttcccaacat taaagtagtt catcttgtct cctgtaatct ccttttttcc
    30841 atcttattct aacaattctc gaataatagc cgcaattttt tccgattctg accattgtaa
    30901 ataatggtga ttccctccta aaatgagttt agtattggaa gtccaatatt ctgattctct
    30961 gtactctttt tctctataag gctgacaaaa aacaaataca ggaatatgag cttctataga
    31021 tacatcctca aaatcttcct cagtaatctc tccagatatc tgaaattctg gatcttgatt
    31081 ttccaactct aagccttttt cttgcattaa ttcccagatt tttttattcg tttcaggact
    31141 aaatgttgct tgagttaagt tcttaaaata aagttcagga ccacactcgt caatcagcct
    31201 catctgctct tccatttctg gataaggatt ttctgaaaaa tcagcaaaca tgactttttt
    31261 agttgtcggt tcaattgcta ctaaagtctg acgcttaatt ggtttctcga gtaatttgca
    31321 agctaaaatt ccactccaac tatgtgcaca aagtatatat tcagaaattc ctaattcttc
    31381 aagtacttca taaaccgcat ctgcaagatt atctagattt tttccagctt ggtcatgaat
    31441 cggactccta cctgtgttcg gaaaatcaat tgtcaaataa ccaattgtag gaggaggttt
    31501 ttcaagtata agtgaaaaat tttcataact tggtagcaaa cctgctccgt ttaaacaaac
    31561 tagcactttc ttttgctttt gataagtaac agagaggcta ccaatttctg tagatacttc
    31621 aaacctcttc ataaagaaat ccactgattc tatataatga attattaaaa atccttatcc
    31681 tttattttat cacgttccaa ggattttctc aagttggagg aaggggacaa tatctctact
    31741 ttcccttcaa taatccttcc aaattatgtt tatgttggta attaatggct gcggttttgt
    31801 ctttctcaaa gacagtcttg gtaaggtcaa tatgattaat agctacgatt gcgacggtgt
    31861 agtaaatgat atcagccagt tctctggcaa gttcctcgtt cgaatcctat cccttctttt
    31921 cgaccagagc gcctattcaa aacctcgact acttctccga cttcctccac taacttcata
    31981 aagagacctt catcagtccg agactgctgt taatgttcga ttaagtagtc ttggaattgc
    32041 ctaaacgttc aatcttttat agtatattga aactagaata gtacaccttt acttctaaaa
    32101 cattgttaga aatcgatttg actgtcctga tcgatttgtc ctgttcttgt ttcattttac
    32161 tatatcttct attccacaca aaaaagcgag acatccgtcc cgcccttctt atttttcgtc
    32221 aataacgatt cttacttttt tgtattcagt tgggacagag tagacaatcg ttcttatcgc
    32281 agaaatagtg cgacccttac gaccgattac acgacccaca tcgctttgat caagattcaa
    32341 atgatattcc aaaaattctg gtgtatcctc aatcttgata gttaaggcat ctggttgtga
    32401 aattaagggt ttcacaatcg caataatgag attttcaatc gtatccatct gtcaacctac
    32461 tttaaactta ttttgaaaat ttagaatcgt ggaatttttt caatacgcct tcttttgaaa
    32521 ggatgttacg tactgtgtct gaaggttgag ctccattagc caaccatgca agaacgcggt
    32581 cttctttcaa agttacttgg ttttcagcaa caagtgggtt gtaagttcca actgtttcga
    32641 tgaaacgtcc gtcacgtggt gaacgtgaat ctgctacgtt gatacggtag aaaggttttt
    32701 tcttagaacc catacgagtc aaacggattt taactgccat ttttaaagtc tcatttcttt
    32761 aattttttat ttcggtgaaa tagctgagct atttagcaca tgttctatta tagcagattt
    32821 ctggcatgtg tcaagaaaaa aacttgacag actataaatt tcttaaagta tttagaaatt
    32881 tgttagaata ggaaataaat gtttgaaaga tgctatccgt gaatactatt ttagaaaaat
    32941 tttataaaga acaccaagtc aaacccttca tatcgcctga acgagagttg gatacttggc
    33001 ttctaagtcc caagcccgtt cccaagcgaa atatggactt attagtagat gattcactag
    33061 caggagatat cattttacta tggagaatcc agtttggtac ttttaccact gaaacatgat
    33121 tttaaaaact cccattcgaa tatgattcaa aattcgctta gaatcaatgt tttcccactt
    33181 tttcaaaaaa taaattttca tctaatttaa ataactttca attaagtgtg aattttgctt
    33241 aaaatcatta aaaaacaccc catggtgtga accatgaagt gttgtaaatg ctgtgttgta
    33301 gctagttctt agcgtttaga gaactggcta gctttacgag ctttcttaag atctggtttc
    33361 caaaatactt tgagtacact tatggtattg atgcccctaa acatctaaaa actctggtag
    33421 aaaaagggta tgctcttgtc gaaacttctt ttgactcact tgaccatcta aataatacca
    33481 tgaaaaagaa tatcctgaag aagaagggag ttactggact ttctaaaatg aaatcttgcg
    33541 acctggatca agcccttcat gagcattttt cagaagaaga attagctggt cactttcatg
    33601 tccttctatg gacttttttt acaatggcat tgctatcaca cccaatacct atctaagcgc
    33661 ctggttcgta aactttattg cagctcttcc tctaaatttc ctaattgttg aaccaattgc
    33721 ccgttttata ctaagttctt ttcagaaacc atttactggg gaagaagttg aagattttca
    33781 agatgatgat gaaatcccaa ctattatcta agccagttct gtaaactact aatatttgaa
    33841 atccacttcc ttttagggta caatggttat aaatgaattt ttgagaggat cagaatgaaa
    33901 aaactagcaa cccttctttt actgtctact gtagccctag ctgggtgtag cagcgtccaa
    33961 cgcagtctgc gtggtgatga ttatgttgat tccagtcttg ctgctgaaga aagttccaaa
    34021 gtagctgccc aatctgccaa ggagttaaac gatgctttaa caaacgaaaa cgccaatttc
    34081 ccacaactat ctaaggaagt tgctgaagat gaagccgaag tgattttcca cacaagccaa
    34141 ggtgatattc gcattaaact cttccctaaa ctcgctcctc tagcggttga aaatttcctc
    34201 actcacgcca aagaaggcta ctataacggt attaccttcc accgtgtcat cgatggcttt
    34261 atggtccaaa ctggagatcc aaaaggggac ggtacaggtg gtcagtccat ctggcatgac
    34321 aaggataaga ctaaagacaa aggaactggt ttcaagaacg agattactcc ttatttgtat
    34381 aacatccgtg gtgctcttgc tatggctaat actggtcaac caaacaccaa tggcagccag
    34441 ttcttcatca accaaaactc tacagatacc tcttctaaac tccctacaag caagtatcca
    34501 cagaaaatta ttgaagccta caaagaaggt ggaaacccta gtctagatgg caaacaccca
    34561 gtctttggtc aagtgattga cggtatggat gttgtggata agattgctaa ggccgaaaaa
    34621 gatgaaaaag acaagccaac tactgctatc acaatcgaca gcatcgaagt ggtgaaagac
    34681 tacgatttta aatcttaaaa accaaaaaaa tacagtatcc acattcggta ctgtatttct
    34741 tttactctca ttcttaagtt aaattattaa aatcccatat ttggtctatc cagccttcat
    34801 aaaagtctgg ctcgtggcag accataagga tagatcccct atattctttg agagcgcgtt
    34861 tgagctcatc ctttgcatcc acatccaaat ggttggtcgg ctcgtccagc actaaaacgt
    34921 tgttttcacg attcatcaag agacagaaac gaaccttggc ttgctctccc cctgataata
    34981 cttgaatctg gctttcaata tgtttggttg tcaaaccaca acgggcaagg gctgcacgga
    35041 cttctgcttg attaagggca ggaaaggcat tccagacagc ttcaagagga gtttggcgat
    35101 taccgccttc tacttcctgc tcaaaataac caagttctaa ataatctcca cgctccactt
    35161 ccccagcgat tggcgagata atgcccaaga gactcttcaa gagagttgtt tttccaatac
    35221 cattagcacc aataatcgca accttttgat tgcgttcgaa ggtaagattt aaaggcttag
    35281 taagaggacg gtcgtaacca atttgcaagt tcttggcttg gaagataaag cgccctggtg
    35341 tacgagctgg tttgaaatca aaggatggtt ttggtttctc actttggagt tcgataatat
    35401 ccatcttatc caatttcttt tgacgagaca tagccatatt acgagttgca acacgggctt
    35461 tattacgagc cacaaagtcc ttgaggtctg caatctcttt ctgctggcgt tcgtaggctg
    35521 cctctagctg agatttcttc atagcataaa cttcttggaa ctggtagtag tcaccagagt
    35581 aacgcgtcag ctgttgattt tccacatgat agacaatatt aataacgtca ttgaggaatg
    35641 gaatatcgtg cgaaatgaga acaaaggcat tctcatagtt ttggagatag cgcttgagcc
    35701 aatcaatatg ctcagcatcc aagtagttgg tcggctcgtc caacagcaag atatcaggct
    35761 tttcaaggag aagttttgcc aaaagcacct tggttctttg cccacctgac aaagaagtta
    35821 catccgtatc catgccaaag tccataacac caagagcacg cgctacttcg tcaatcttag
    35881 catccaaggt atagaaatca cgactctcca gacggtcttg aagttctcct acttcttcca
    35941 tgagagcatc aacatccgcg ccgtcttcag ccattttcat atagaggtca ttgatacgag
    36001 cttcagcttt gaaaagctca tcaaaagccg tacggagaac atcacgcacc gactgtcttt
    36061 cagcaaggac agagtgctga tccaagtaac cagccgtcac atatttggac cactcaacct
    36121 ttccttcatc tggcagcatt ttaccagtca cgatactcat aaaggttgat tttccttcac
    36181 cattggcacc gaccaggccg atatgttctc ccttgaggag acggaaggac acatcttcaa
    36241 aaattgcacg gtcaccaaaa ccgtgactca gatttttaac ttctaaaata ctcattttaa
    36301 ttccttacct tgtttttatg taatcgttta taaaggagcc aagccagata gccacccaaa
    36361 gtgttggtcc acaaatcatc aatctcaaag acgcgattga aatcaaagaa aaagtccaag
    36421 attaattgcg tacactcgat tccaagactc acaagaaaac taaaaagaag gacctttttt
    36481 gttttccgca aatttggaaa tagataaagg agttggaaaa tcagaggaaa aaacaagaag
    36541 acattgagga tattttgtaa aaaaatccaa cataattgtc caatgtcact cacttcgccc
    36601 agtttccaga gagaattgaa aggagtcaaa agaaaaacca ggcgtccaag atgctgaata
    36661 cctggagttc ccactcccac ggtagattgt tcttgaggag taaagcaaaa acagacaatg
    36721 caaatgctat agaaaatgac tccccagacc aaaatatgat tataagtctt cttcatcatt
    36781 aaggatttac cgctgcgact gccttctggc ggtcacgttt cattgtgtta gagcgcaatt
    36841 gtccacaagc tgcgtcaata tctgtaccat gctcttgacg aaccacacag ttgacccctt
    36901 ttttcttaag cgtatcatag aaagccaaca cgcactcttt gggactacgg ctatattggt
    36961 catgctcact aactgggtta taaggaatca agtttacata agacaatttc ttgatgttct
    37021 tgagcaattc agtcaattcc aaggcttgtt ctacaccgtc gttgacttca ttaagcatga
    37081 tatattcaaa ggttacacga cggtttgttg tctcaatgta gtattcaata gcagcaaaga
    37141 gtttttcaat cggaaaggca cggttaatct tcatgatact tgaacgaagt tcattgttag
    37201 gtgcgtgaag agacacggca agattgacct gaaccccttc atcagcaaag tcacgaattt
    37261 tatgagccaa acctgaggtt gaaaccgtga tgtgacgagc accgatagcc attcctttat
    37321 catcattgat agtacgaaag aaattcaaga cattgttgta attatcaaag ggctcaccga
    37381 ttcccatgac aacgatatgg ctgatgcgtt catcctgacc acgctcatca aagtatttct
    37441 gaaccagcat gatttgcgct acgatttcac cgttattgag gtcacgttgc ttcttaatca
    37501 aaccagaggc acagaaggta caaccgatat tacagccgac ctgagtggtc acacagacag
    37561 ataaaccata gtgttgacgc atgagtacag tctcaattaa cataccgtcg ggcaattcaa
    37621 agagatattt gactgtacca tcagcagact cttgcacaat acgttgtttc aagggattga
    37681 ccacaaactg gtcattgagc ttagcaatca aatccttgga aaggttggtc atttcttcaa
    37741 atgactgcac acgtttacgg tagagccatt cccagatttg atctgcacgg aatttctttt
    37801 ctccctgctc caatacccat tcctgcatgg tttgatgtac caaactatga attgagggtt
    37861 tcatttcttc tccttattct ctactcactt ctgacgaatg acaaaatgac gttgtccctt
    37921 gtcgtctttc tgacgacgtc tatttttctt atctgcattc gactttcgtt tagtttgagt
    37981 cggtttcttt ccttttctag aaggtgtttc ttcttccgtc ttacgcattt tcttgtcaaa
    38041 tgatgctcgc ttaggggctt cattttctaa gacaaaatag gcacaaccat aactacaata
    38101 ctctaaaagg tagtcttgta aacgactgat tttttcaagt ttttcttctg ttcggtcatc
    38161 cttgtaaaaa cctcgtaggc gaagctgttc gttgctccag tcccccacga tataatcaaa
    38221 cttggttaat acttctgaaa aacgctgatt aaaagtcgtc acatcaaagg catccttgat
    38281 attttcaacc aaggaaaaag ctatcccttc cgtttcgacc ttgtccccgt gtaaatggaa
    38341 ctccggacca ggaaacttgt tatagttgta taattcaggt gcaatttctt ttcgcataga
    38401 tatccttttt tcacgattac ttaatacttt attctaccat aatttctagc agttagcacg
    38461 tttctcataa aaatgaaaaa agtctgacga ttttgtcaga ccagaatctt ataacctaaa
    38521 aagagaagaa caattcttcc ctccaactat cattatttag cagctgcgta caattcatct
    38581 actttattcc agttgattac tgaaaagaaa gctttgatgt agtcaggacg cacgttgcgg
    38641 tatttcacgt agtaagcatg ttcccaaacg tccaagccca agattggttt tttaccttct
    38701 gagattggtg tgtcttggtt tgctgttgaa gtcacttcaa gtttcccttc tttgttgaca
    38761 accaaccatg cccaacctga accaaaacga gttgttgctg ctgcagtgaa ggctgcttgg
    38821 aattcttcaa atgaaccaaa tgttgcatcg attgctgctg ccagttctgc tgaaggagct
    38881 gttttctcgg gagtcatcaa ttcccagaaa agagcgtggt tcaagtgtcc gccaccattg
    38941 ttgataagtg cttgacggat atcagctggg atagattcta catcagcaag caaggcttca
    39001 aggtcttcac cgatttcagg gtgtttttct aaagctgcat tggcattgtt gacataagtt
    39061 tgatggtgtt tgtcatggtg caaatgcatt gtttccgcat cgatgtatgg ttccaaagcg
    39121 tcgtatgcat atggaagttc tggtaagata atagccatct gtaatacctc tttttctttc
    39181 tatatgaaaa tgataacgca aacattcact tttttcaagt tttttgctta tagataagga
    39241 aatcactgac atactttcta ataaaataag caaatcagca agaaacccac gacaagatag
    39301 tcctgacgcg ggttgtagtt tcaacaaatg tcaattgacc tgactagcaa tctgtaagag
    39361 tgccttttca aaaaggaaac ctttttcata aagacctgtc ttaatctgat agtctgtctc
    39421 aatcaaatag gaaatagctt gcttcaaaaa gctcaaagaa agtcctctcg aatctcttaa
    39481 tgcaaacttg atttgataag gatttgggtt acgtcccaga taactaccta aactacttgc
    39541 aatctgcgat tctgtttggc cagactccgc caaaatcttc acctgagtaa aagtccgaaa
    39601 ttgtcccagc atgactgcaa tcagtttgat ttcatcttcc ccttgcaagg tcaagtctct
    39661 caccaaatcg cgcgcctgat ccatcttttt agtcagaata aactgagtta aatcaaaaat
    39721 attgtcctgc aaggtcttgg gaattgcgtt aacaatatcc tcttcctcaa taacagaatt
    39781 cgccttatag gactgtaaaa agagaagatt tttctggatt tcgctaaatt gaaaccccga
    39841 cttgatgagg agattttcaa aagaatgatt ggtaaactgc agaccttgtt tctgactcca
    39901 cttttggaag tactggcgca attcttgttc ttttacttct actgcatcga aggccttggc
    39961 atcacgctta agtaatttga ctaaccgtct tttgctatcc agctttcctt ctgcaaagat
    40021 tatcaacttg gttgttggag aaggattgtc aaggtattcc tcaaatgact taagctcatc
    40081 atctgtcaaa aagcgtttct tagcagtcgt gatatccata aaataatcta atatcacgat
    40141 tttctcatcc gcaaagaaag gaaggctgac caactccagt tccacatcct tgtaaactac
    40201 ttctttcata tcaaagtagg caaagttgag gtcagcagaa tcatacccaa tctgtttcaa
    40261 catttgactc ttcatcactt caaactgacc ctgatctgtc cctgtaaata ggctcaggct
    40321 cggtaaattt gataaagtca acttctgact ttcttcaatg gctagcatcg tctctccttt
    40381 cttcagattt ttcgatttaa tttagtcaat atagcgcaat ttcccacgga aatcttctaa
    40441 gctctcgtag cctttttcca ccatgattgc tttcagttca ttggtaatgc ggtcaaaagc
    40501 actgacgcct tctttgtgaa gggtcgttcc cacctgcacc atacttgctc cacagaggat
    40561 gtgttcaaag gcatctcgac cagtcagaac gccacctgtt ccgataattt ggatttgagg
    40621 atttaaacgt tgataaaagg cgtgaacatt ggctaaagca gtcggtttga tgtattctcc
    40681 accaattcca ccaaaaccat tcttaggccg aataacgaca gattcgtctt ctatatagag
    40741 gccgtttccg atagagttaa cgcagttgac aaacttgagc ggatatttgt tgaaaatagc
    40801 tgccgcttgg tcaaagtgaa caatatcaaa ataaggtggc aatttaattc caagaggttt
    40861 ggtgaagtaa gcaaacactt ctgccaaaat ccggtctgtt gtctcaaaat cataggcaat
    40921 ctgaggttta cctggaacat ttggacagga aagatttagc tcagtcagac cacgaaaatc
    40981 actctcttgg acttttttca aaatagtatg ggtttcctct ggagacatgc cgaccagaga
    41041 taagaagaaa gttcggttcg actctttttc ctgcaaatct aaaagataat ccaaataata
    41101 gtctaagcca ttatttggca agcccataga gttgatggaa ccaagtggaa catcttggta
    41161 gcgtggctca ggattcccct gacggaagtc caaggtcgct gtcttagtaa caaaggttcc
    41221 tgccgctgag tttttgacct cttctaactc ctctatcgtc atacaagcca cacctgctgc
    41281 attcatcaag caattgtcaa actcaaaacc agcaatttgt gttttcgttg ataccatgat
    41341 tctaatcctc cagatttctt ttattgctaa tattatacca taattttaat tctaggttta
    41401 ctagattctg tggttctatc tatttattcg gaaaagaaag tctagtacag aagtcatgct
    41461 atggaaacag ctagtgtatt agaagtttga acaaggcttg ggtaacagac ttttgaatga
    41521 agtccaagca acaaggagaa atctcaacca tgagtccatt ccatgactct gttgctctag
    41581 aaaaccttgc aatagtcaag tgataacgaa taaaactttg gattgaaata tccccaagat
    41641 tatctattac gaggagaaat cttaaccttt atcattttta aagaaatata gtaaaatgaa
    41701 acaagaacag gagaaatcga tcaggacagt caaattgatt gctaacaatg ttttagaagt
    41761 tgaggtgtac tattctagtt tcaatctact atacatgtaa atctctactt tcgcaaaatg
    41821 gattttcaga acagtatccc agtatagtta aaaactataa aaaagagagg acgttcccct
    41881 cttaaaatct tattttactg cttctttcaa agcatctacc ttatccaaac gttcccatgg
    41941 aagatcaata tctgtacgtc ccatgtgacc gtaagccgat gtttgacggt aaattggacg
    42001 cttgaggtcc agcatttgga taatccctgc agggcgaagg tcaaagattt gacgagccgc
    42061 tttttcaagt tgactttcag ctactgttcc tgtaccgaaa gtatcgatac gaacagaaac
    42121 aggttgcgca acaccgatag cataggccaa ctgcacttct gccttcttag caaggtctgc
    42181 tgcaacgata ttcttggcaa tatagcgagc cgcataagag gctgaacgat ccaccttagt
    42241 cgcatcttta ccagagaagg caccaccacc atgacgagag tagccaccat aagtatctac
    42301 aataatctta cgaccagtca aacctgagtc cccttgagga ccaccgatta caaaacgacc
    42361 tgtcggattg ataaagaatt ttgtcttatc atcaagataa gaagatggaa taacttcttt
    42421 gatgaccttg tcaatcacat cttgatggat ttgttcatta gtggcctctg gatcatgctg
    42481 agtagaaata acgactgtat ctacacgtac cggacggtca ttttcatcgt actcaactgt
    42541 aacttgtgat tttgcatctg gacggagata gctaatttct ccagacttac gaagttctgc
    42601 cagacgacga accaatttat gactgagtgc aattggcaat ggcataagct cttctgtttc
    42661 atctactgca aatccaaaca tgagcccttg gtcacctgct ccaatcaagt ccagtggatc
    42721 ttgatcagca tttccacgaa cctccaaggc ttcgttaaca ccttgagcga tgtcaggaga
    42781 ttgttccacc aaagatgggt gtactcccac cgtctcagca gaaaatccat attctgtatt
    42841 ggtataacca atctctgcaa tggtatcacg aaccacacgg ttaatatcca cataggcatt
    42901 tgtagaaatt tcaccaaaaa cgtggacaga accagtatat acagctgttt cagcagcaac
    42961 gtgcgcctct ggatcctttg ctaaaatagc atccaaaatc gcatctgaaa tttggtctgc
    43021 aatcttatcc ggatgcccct cagatacaga ttcagacgtg aataatttac gctctgacat
    43081 aaaaatgtcc ccccttaaaa aatattgtta tagagttaca aagtactgat aggaaaactc
    43141 ttcgtaataa atacctacca tcttatcggg actatataac tttatcatta taccattttt
    43201 aatcgcaatt tgcagtcttt aatgtagtgt ttcggtttag atagttcgtt ctcataaata
    43261 aaaaagttgg agctcaatac tccaacaatt tctattaaaa tgtttggcgt ttagctttac
    43321 gctctgcacg accgcgagcg cgattttcag cacgcttggt tttacggcgc ttttcatcaa
    43381 ccgcccattg aattttcttc ttataacccg gtttgacttt tttctttttc tttttaacca
    43441 aaccaatcat ttcgatatca agtttatctt gttttttctc acggttggca cgacgatcac
    43501 ggtcataggt atcttgaaat tccccgtctt tgaccatctt aggactaaac ttgattccca
    43561 atttctccaa ctcacggata tccgagtcat cacttggctg ataaagggta atagctgtac
    43621 ctggtaggcc atttcgtcca gtacgaccaa cacgatgaac aaaaaaagat aagtcttgcg
    43681 gaatggcatc attgatgaca tggctgacac cttcaatgtc aatcccacgc gctgccaaat
    43741 ctgttgcgac aatatactca aaatccagat tttgcacctg attcatgatt cgcttgcgtt
    43801 cacgaggggc aatatcgcca tggatttttg caaccttcaa gccttgagca gtcagatatg
    43861 aatgcaattc atcagcacgc gttttagtgt taacaaaaat cattgccaaa tacggctgca
    43921 tcaactgagt caactggtaa atttgagcat tcttatcatg tcccttggtc gaaatcaacc
    43981 aattatcaat ggtgtcagaa ataaccgttt tggtcttaat tttctccata acaggatttg
    44041 ataagtattt tttcaagaat ggttgcagtt tttgtgggat agtcgctgag aagaccatga
    44101 attgcaagtc ttttggaaga ctgccagcaa tcttatcaac agtttccaag aatcccatat
    44161 ccaaggtcat atctgcttca tcaacaacaa atgtcttggc tttatgaata gctaaatcac
    44221 cagatttaac caagtcgtag atacggcctg gtgttccaat aacaatatga ggctgattgc
    44281 ttgccaattt ctcaatctgg cgagccttat ccgtaccacc cacataatta accacacgaa
    44341 cttcgacatc tgagtgagct gaaatctgac gcgctacttg gtaaatttga gtagccaact
    44401 cacgactcgg tgcagtaatc actgcttgta cactatcgct agcttcatct aattgctgga
    44461 aaatcggtaa caagaaagta tgagtcttac ctgaacctgt ttttgattct cctactaggt
    44521 cacgacctgc caaaacaata ggaatcaact tgtcttgcac ctctgttgga gttgtaaatt
    44581 ttaactcctt caaggcttct ctaatatagt ttttaaattg aaatttcgta aatgacataa
    44641 catcctcgat tctatctacc ttatcaatta taccatattt tattccatta cagtagtctc
    44701 acttatttag gctatttcca gtagcttctc tagtaagaaa aggctggaat tttatagttc
    44761 caacctcttt tcagttatta tttccagttt aatatagcat tcaagccata gtgatcactc
    44821 acttgtggac tcttgttacc atcaaatacg acatgtaaat tttccaccgc taactctttg
    44881 gtagtaaaga cataatcgat tcgaaggggt tcagtgttcc ctttccagcc atcaatttca
    44941 ggcggaacag tatagctacc acttttctct tgagcaactt caaatgcgtc ttgtaagcct
    45001 aatggactag ctaaaatagc ttggtaacct tcctgtccag ccggattgtt gaaatctcca
    45061 gctagtaaaa gtggcttgtt caattttttc aagacagcct caaatcgtgc ccattcttct
    45121 tggaaacctt tatcccacca agagagatga acactggcaa ctgctagctc cttgccatcg
    45181 actacagttt cagctagggc aacacggcga gtatgatagt ctgttggatc atccacatct
    45241 gaaaccaaaa tttctctggc ttcaataggt gttttagaca agatagccac accttcgtgg
    45301 tagcggttat agccgatatg gttataggcc caggtccagt agtaattttt cccttgctca
    45361 gacaactttt caaccaagag tctaacataa tggtcttggt gaataggctc agctgctggc
    45421 aaagcttgat aaaggtcatt aacctccacc tctgacgagg tcatctcctg attgatttct
    45481 tgaaaacaaa tcaaatcata gtccttttca agaatatctt caagcaaaat ctggaatttt
    45541 tcctctgctt ctttctccat ccaactgtga gtattgagtg ttaaaaattt cattcttttc
    45601 tcccaaaata agaggttgga atacagcttc caacctcaag tatattacaa ttctacttta
    45661 gcaactgctg ttttagctgc aagagaacct gttttttcta acttaactga tttaattgca
    45721 tcaccatttg tgaagacaac tactgttgaa gtttcacgtc ctgctgcacg gatagcatcc
    45781 aagtcagctg tgacaaggag atctcctgct gcaacttttt gtccttcagc aacatgaact
    45841 gtaaatggtt taccttcaag acttactgtg tccaaaccaa tgtgaaccaa tacttcaaga
    45901 cctgcttccg tcacaatacc aaaagcatgt tttgttggga agatgcttga cacagtacct
    45961 gaaactggag atacaatgtt tccatttgca ggttctactg caaatccatc acccatcatt
    46021 ttttgagcaa atactggatc ctttacttgt tccaaagcaa caacttgacc gtctgctact
    46081 gagtaaactt cctcagtaag atctttgaag tgaacagtgt tttgttgtgc ttcagtcatt
    46141 tggcttggaa gagtttcagg aatgatttca cctgaatcaa ggatatcttg gatatcagat
    46201 ttcaaaatgt cagcttttgg accgtagata gcttgaaccc cttgtccttt catgacaaga
    46261 cccatagctc cttctgcttt ccattgctct gcatttccta ctttatctgc atctttaaca
    46321 gttacacgaa gacgagtcat acatgcatca acatcaacga tgtttacacg tccaccaaga
    46381 aggttgataa tgtttacagc ttgagagcct gctgcaactt tcacttcgct gctggtttct
    46441 tctgaacctt cagcagtttc gtagtttccg ttgcgccctg gagttgcgta gttgaatttt
    46501 tgaatcatga agtttgcgat aaagtacatg attacagcaa agagaacagt tacccaaacg
    46561 aagttaacga tatccatacc aataccagca ctgattgcaa taggtgtacg agtcaagaac
    46621 tcgattgaac cgaatgagtg catacgtagg tttacgacgt cagccatagc gaaggcagca
    46681 ccttgaacaa gtgagtaaac aagatacata ggtgttgcga tgaacatgaa catgtattcg
    46741 attggttcag taacccctgt caagaatgtt gcaagagctg ttgcaatcat catacctttg
    46801 tatttatgtt tcttgtcagc atcaacatta cggtagatag caacaatcac acccatcaag
    46861 ataccgaatg aaccgatcat ttgtccaact ttgaaacgag ctggatgtac tgtatctaac
    46921 aagtgttgat attgactagc atcagtacct ttaaggttta caaggtctgt tacccatgca
    46981 agccatagtg ggtcttgacc gaatacttga gtacctttag ctgcaccagt taaaatgtca
    47041 taagtaccac caagagctgt gtagttcatt gggatagtca acatgtggtg aagaccaaat
    47101 ggcaagagca aacgttccaa agtaccatac aagaatggtg caagaattgg agcagtttct
    47161 tgtgagttgg caatccagat accgaagtta ttgatacctg tttgaactac tggccagaaa
    47221 gcagcaagta gaattgcagc gattgctgaa cgaagaataa ctacaaatgg tacgaaacgt
    47281 ttcccgttga agaatgaaag tgcatcagga agtttacgga agttgtagta tttgttgtaa
    47341 gcagttgccc ctacaaaacc tgagataatc cctacgaata cccccatgtt caaggctgga
    47401 gcttcaagaa cactgataaa gtaatcagca actttgattg aaccaccaaa gaaagtagtt
    47461 accatagcat ctggattttt caacatatcg cctgatacac caaagattgt accagtgata
    47521 cggttaatca agatgaaggc aagaccagcg gcgaaagcac caccagcacg ttctttagcc
    47581 cagcttcctc caatggctag ggcaaacaaa atgtgaaggt taccgataac cccccaaccg
    47641 atttgctcaa gaattccacc tgtgatgaca agtggtgcaa aggttgggtt aatcatcacg
    47701 atagacttac cgattgaaat catcaaacca gcagccggca taaccgcgat aactaccatc
    47761 aaagccttac cgaatttttg ccaaaattcg aaagacaaga catttttgaa tgtatctttc
    47821 atcattcaaa tctcctttat ttttatttag gcaaacgttt tacgaaaacg tttgcac
//
LOCUS       DAVHEW010000005        44627 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVHEW010000005 DAVHEW010000000
VERSION     DAVHEW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.005"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            174..1163
                     /gene="manX"
                     /locus_tag="VVL53_000288"
     CDS             174..1163
                     /gene="manX"
                     /locus_tag="VVL53_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU4182508.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1191..1994
                     /locus_tag="VVL53_000289"
     CDS             1191..1994
                     /locus_tag="VVL53_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU4182509.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            2018..2929
                     /locus_tag="VVL53_000290"
     CDS             2018..2929
                     /locus_tag="VVL53_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4182510.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2985..4319)
                     /gene="pepC"
                     /locus_tag="VVL53_000291"
     CDS             complement(2985..4319)
                     /gene="pepC"
                     /locus_tag="VVL53_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU4182511.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4537..5259)
                     /locus_tag="VVL53_000292"
     CDS             complement(4537..5259)
                     /locus_tag="VVL53_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU4182512.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(5432..5506)
                     /locus_tag="VVL53_000293"
                     /pseudo
     CDS             complement(5432..5506)
                     /locus_tag="VVL53_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(5527..5757)
                     /locus_tag="VVL53_000294"
     CDS             complement(5527..5757)
                     /locus_tag="VVL53_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU4182513.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(5767..6993)
                     /locus_tag="VVL53_000295"
     CDS             complement(5767..6993)
                     /locus_tag="VVL53_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU4182514.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALVPDPSPISQSGITFFNT
                     LFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMDIDGIR
                     EDGTRVPLFRNGNWAN"
     gene            complement(7015..7167)
                     /locus_tag="VVL53_000296"
     CDS             complement(7015..7167)
                     /locus_tag="VVL53_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182515.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(7188..7466)
                     /locus_tag="VVL53_000297"
     CDS             complement(7188..7466)
                     /locus_tag="VVL53_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU4182516.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARN
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(7459..7722)
                     /locus_tag="VVL53_000298"
     CDS             complement(7459..7722)
                     /locus_tag="VVL53_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU4182517.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(7824..12215)
                     /locus_tag="VVL53_000299"
     CDS             complement(7824..12215)
                     /locus_tag="VVL53_000299"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU4182518.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(12319..14400)
                     /gene="fusA"
                     /locus_tag="VVL53_000300"
     CDS             complement(12319..14400)
                     /gene="fusA"
                     /locus_tag="VVL53_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4182519.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(14825..15295)
                     /gene="rpsG"
                     /locus_tag="VVL53_000301"
     CDS             complement(14825..15295)
                     /gene="rpsG"
                     /locus_tag="VVL53_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU4182520.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(15315..15728)
                     /gene="rpsL"
                     /locus_tag="VVL53_000302"
     CDS             complement(15315..15728)
                     /gene="rpsL"
                     /locus_tag="VVL53_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU4182521.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16145..19987
                     /gene="spuA"
                     /locus_tag="VVL53_000303"
     CDS             16145..19987
                     /gene="spuA"
                     /locus_tag="VVL53_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU4182522.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            complement(20087..21136)
                     /locus_tag="VVL53_000304"
     CDS             complement(20087..21136)
                     /locus_tag="VVL53_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU4182523.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(21255..23063)
                     /gene="glmS"
                     /locus_tag="VVL53_000305"
     CDS             complement(21255..23063)
                     /gene="glmS"
                     /locus_tag="VVL53_000305"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU4182524.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(23256..24635)
                     /gene="bglA"
                     /locus_tag="VVL53_000306"
     CDS             complement(23256..24635)
                     /gene="bglA"
                     /locus_tag="VVL53_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU4182525.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(24734..26587)
                     /locus_tag="VVL53_000307"
     CDS             complement(24734..26587)
                     /locus_tag="VVL53_000307"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU4182526.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDDYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(26600..27859)
                     /gene="rseP"
                     /locus_tag="VVL53_000308"
     CDS             complement(26600..27859)
                     /gene="rseP"
                     /locus_tag="VVL53_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU4182527.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PEMAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(27881..28684)
                     /locus_tag="VVL53_000309"
     CDS             complement(27881..28684)
                     /locus_tag="VVL53_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU4182528.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIILMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(28693..29451)
                     /locus_tag="VVL53_000310"
     CDS             complement(28693..29451)
                     /locus_tag="VVL53_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU4182529.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(29682..30254)
                     /locus_tag="VVL53_000311"
     CDS             complement(29682..30254)
                     /locus_tag="VVL53_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU4182530.1"
                     /translation="MNTVKNKQEILEAFRESPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEELDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(30235..31206)
                     /gene="ruvB"
                     /locus_tag="VVL53_000312"
     CDS             complement(30235..31206)
                     /gene="ruvB"
                     /locus_tag="VVL53_000312"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU4182531.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNDLEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            31368..31460
                     /locus_tag="VVL53_000313"
     CDS             31368..31460
                     /locus_tag="VVL53_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU4182532.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(32212..33468)
                     /gene="ltrA"
                     /locus_tag="VVL53_000314"
                     /pseudo
     CDS             complement(32212..33468)
                     /gene="ltrA"
                     /locus_tag="VVL53_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(34262..34678)
                     /locus_tag="VVL53_000315"
     CDS             complement(34262..34678)
                     /locus_tag="VVL53_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182533.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQTEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPSYQRQGIGSSLMKEALGNFKEAYQVQ
                     LATEETEKNVGFYRSMGFEILSTYDCTGMIWINREK"
     gene            complement(34690..35385)
                     /locus_tag="VVL53_000316"
     CDS             complement(34690..35385)
                     /locus_tag="VVL53_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182534.1"
                     /translation="MPVNEYGQMIGESMEAYTPGELPSFDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQESVADMEELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLACYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKARLEIWLRPENF
                     DKNGRQHKSLREL"
     gene            complement(35579..38080)
                     /locus_tag="VVL53_000317"
     CDS             complement(35579..38080)
                     /locus_tag="VVL53_000317"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU4182535.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFIVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEISLADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            complement(38535..39623)
                     /locus_tag="VVL53_000318"
     CDS             complement(38535..39623)
                     /locus_tag="VVL53_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU4182536.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(39641..40309)
                     /locus_tag="VVL53_000319"
     CDS             complement(39641..40309)
                     /locus_tag="VVL53_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU4182537.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(40328..42766)
                     /locus_tag="VVL53_000320"
     CDS             complement(40328..42766)
                     /locus_tag="VVL53_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU4182538.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNLLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(42917..44215)
                     /locus_tag="VVL53_000321"
     CDS             complement(42917..44215)
                     /locus_tag="VVL53_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4182539.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            complement(44232..44540)
                     /locus_tag="VVL53_000322"
     CDS             complement(44232..44540)
                     /locus_tag="VVL53_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4182540.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
BASE COUNT        13050 a        10275 c         8250 g        13052 t
ORIGIN      
        1 taaataagaa ttcaagatta ctcctcaaaa actcaacata tcaacataca aaaataaaaa
       61 ttttttaatt ttttatgata aatagttgct ttttttatga aaacggttta tacttaaaca
      121 gtcttaaagt tttcttaaat gaaaactaaa acaaaaaagg aggaatgaca ataatgagta
      181 tcggaatcat tattgcgagc cacggcgaat ttgctgcggg tattcatcag tcaggatcta
      241 tgatctttgg tgaacaagaa aaggttcaag ttgtgacctt tatgccaaat gaaggtcctg
      301 atgatctata cgctaagttt aataacgctg ttgctgcatt tgacgcagaa gatgaggttc
      361 tagttttggc tgacctttgg agtggttctc catttaacca agctagtcgc gtgatgggag
      421 aaaatcctga gcgtaagttt gccatcatca caggacttaa cttaccgatg ttgattcaag
      481 cctacacaga gcgcctcatg gacgctgctg caggtgtaga aaaagtcgct gctaatatca
      541 ttaaagaagc caaagatggc atcaaagctc ttccagaaga gctaaatcca gtcgaagaag
      601 ttgcaagcgc tgcagctgct ccagttgccc aaactgctat cccagaagga actgttatcg
      661 gagacggtaa attgaaaatc aatcttgccc gtcttgacac acgtctactt cacggtcagg
      721 ttgcaactgc ttggactcca gattcaaaag caaatcgtat catcgttgct tcagataacg
      781 tggctaaaga cgaccttcgt aaagaattga ttaaacaagc agctccaggt aatgtcaagg
      841 ctaacgtggt tccaattcaa aaactgattg agatttcaaa agacccacgt tttggagaaa
      901 cacatgccct tatcttgttt gaaacacctc aagatgccct tcgtgccatc gaaggcggcg
      961 tgccaatcaa gactcttaat gttggttcta tggctcactc aacaggtaaa acattggtca
     1021 ataccgtttt gtctatggac aaagaagacg ttgctacatt tgaaaaaatg cgtgacttgg
     1081 gtgttgaatt tgatgtccgt aaagtaccaa atgattctaa aaaagatttg tttgacttga
     1141 ttaacaaagc caatgtcaaa taagccatta tttatgaaag gattttaaac atgtctatta
     1201 tttctatggt tttagtagtc gttgtagcct tctttgcagg tcttgaaggc atcctcgacc
     1261 agttccaatt tcaccaacca cttgtagcct gtacccttat tgggcttgta acaggtcact
     1321 tggaagcagg gattatcctc ggtggatcgc ttcaaatgat tgcccttggt tggtcaaata
     1381 tcggtgctgc tatcgctcct gatgctgcac ttgcttctgt cgctgctgcc attatcatgg
     1441 ttcttggtgg tgactttacc aagactggta tcggtgttgc ccaagcggtt gctatccctc
     1501 ttgctgtagc tggacttttc ttgacaatga ttgttcgtac aatttcagtt ggtttggttc
     1561 atactgcaga tgctgccgct aaaaaaggtg acttcggcgc tgtggagcgt gcgcatttca
     1621 tcgcgctact tttccaagga cttcgtatcg cgcttcctgc agctcttctc cttatggtac
     1681 caactgaaac tgtacaaagt atccttagtg ccatgccaga ctggctcaaa gatggtatgg
     1741 ctatcggtgg tggtatggtc gttgccgttg gttacgccat ggttatcaac atgatggcaa
     1801 ctcgtgaagt atggccattc ttcgctcttg gtttcgttct cgctgctgtg tcagatatta
     1861 ctctaatcgg attcggtgct atcggcgttg ctatcgctct tatctacctt cacctttcta
     1921 aaactggtgg aaatggtggc ggaggagccg caacttctaa cgacccaatc ggcgatatcc
     1981 tagaagacta ctaagataag aaaggactga aaacatcatg actgaaaaac ttcaattaac
     2041 taaatcagat cgtaaaaaag tttggtggcg ttcaaccttc ttacaagggt cttggaactt
     2101 tgaacggatg caaaacttgg gctgggctta tacactcatt ccagctatca aaaaactcta
     2161 tactaaaaaa gaagatcaaa tcgctgctct tgagcgtcac cttgagttct tcaacactca
     2221 tccatacgta gctgctccag tcatgggggt tactcttgcg cttgaagaag aacgtgctaa
     2281 cggtgtggaa atcgatgacg ctgctatcca aggggttaaa atcggtatga tgggacctct
     2341 tgctggtatc ggtgacccag tattctggtt tacagtacgc ccaatccttg gatctctcgg
     2401 tgcttcactt gcccttactg gcaatatctt ggggccactc ctcttctttg ttgcatggaa
     2461 cttgattcgt atgtcattct tgtggtatgt tcaagagatt ggatacaagg ctggatcaga
     2521 aatcactaaa gatatgtctg gtggtatcct tcaagatatc actaaaggag cttctatcct
     2581 tgggatgttc attcttgctg tccttgttca acgctgggta aatattaaat ttgctttcga
     2641 tgtttctaaa gttcaactag atgaaaaggc ttatatccat tgggataaat tgccagaagg
     2701 gtctaaaggt atccaagaag cattcgcaca agtaggacaa ggattgtctc aaactcctga
     2761 aaaagttact actttccaac aaaacttgga tatgttgatt cctggattat caggactact
     2821 ccttacttta ctttgcatgt acttacttaa gaaaaaagta tctccaatca ctattatcct
     2881 tgccctcttc gcagtgggta ttgtggcaca tgttcttcac atcatgtaat caagcaacta
     2941 aaaaggaacc aggttctaaa atctgattcc ttttttctat gcttttattc agccaaggct
     3001 cccattggat cccatggtgc aagtacgatt ggttctgctc cataggcagc ttgttcttct
     3061 gctgtcagca attccttacg aacaacgatt tggtatgtgt attcgtccat ccaagcgtct
     3121 gaggcaacaa agtaaccatc tgtaccgacc ttgtctcccc atgagttttc aaccttccac
     3181 ttggttgatt taccattttc gtccaagtca acacctgtca agaccatggc gtgggtcatc
     3241 aagctttcac tatagtccaa acgtccagcc ttgtcttgag taagtttaat gtccatgctt
     3301 gattcaaagt cataaacatc tgtcgcaagg atgccagcct tacggttgct gagctggccg
     3361 acatcagaac caaaccaaac agtctcacct gcttgcattt gggcaatcgc caattctttc
     3421 aagcgctcca ttggaacgtt gatgtagcga actgcacggc taccaaccac attccccaac
     3481 atctcaactg tgtaagattt tccgtaaggt ttatcagcag ttggagcatt gataacagaa
     3541 acgtagtctt ctaaaggaag attgacatat ttcttgtaaa actcttgtgg tgtgattcct
     3601 ttttcacttt tgtagttgtt atctttatcg cgataagcaa agtcaaactt gcgtggtgga
     3661 agtcctaatg acatagcaag aaagttaaag atttcttgca agaggtcttc tttcttagct
     3721 tgaacagtcg cttgatctgc accagaaaca agcaagtcac gcaagatttg agcatcttga
     3781 cgaagcaatt tattaaggat cgcatttagc tcacgactgc tgctagatga aacagactca
     3841 ggataaactg acttaggcac gacaccgtat ttttcaaaga gggaaacgac catatcccat
     3901 tgaccgccat cttgttgagg tgtttggagt aagaagctaa ccttgcggct agtcaattct
     3961 tggtctgaag tcgcaatgac ttgctccaag aaccagtttg atttctcata cttatcccag
     4021 aagaaagtgt gggcttgtga caactcaaag ttctccaatt tgtattgcga gatgagtttg
     4081 tggcggaagg tgttgagagc cgcaaacatc cagcaacgac cagacgcttt ctggttagtg
     4141 accttgtcct tggttaaatc caatgagaaa acaggtgtgt tgtctacatg gctttggcga
     4201 cgttccagag ctgcaaaaat tccgttgtgg ctggcagcat tttcaatcgc ttggtatttt
     4261 acatttgctt catagttggc aaatagttta tcagtaaatg attcttgaat cgcgttcata
     4321 gattcctcct tttattctac agtctattat aactcttttc acaaaggaag caactaaaaa
     4381 acataaaagg cgaggacatt gtccctcacc tttttcgaat ttatactctt cgaaaatcaa
     4441 attcaaacca cgtcaacgtc gccttgccgt actcaagtac agcctgcggc tagtttccta
     4501 gtttgctctt tgattttcat tgagtattaa actaaattaa ataatattag cgcggagaat
     4561 ttctaattct tccttggtca agcgacgcca ttcccctcgt tctaggttct catctaatac
     4621 taaagttccc atagtcaatc gttgcaagtc caccacttcc ttgccacagt agcccaccat
     4681 acgcttgatc tgatgaaact tcccttctgc aatggtcaca cggatttggc tttgattctt
     4741 ttctgtatct atggatacaa gctccagtat agcgggttga caggtaaagt ctttgagagg
     4801 aataccctca gcaaatgtct ccacatcttc ttgggtcatg attcccttga cttgtgccag
     4861 ataagtcttg tccacatgac gcttgggcga aagaagaaca tgagccagct gaccatcatt
     4921 ggtcaagagc aaaagaccat gcgtgtcaat atccaagcgt cctactggga aaacttcctt
     4981 actccgcgcc aagtcatcca acaagtccag aacggttctg tgcttgggat cctcagtcgc
     5041 tgagataact cctttgggct tgttcatcat gtagtagaca aactcttcat actccaacac
     5101 ttgcccatca aagcgaatct catctatttt ttcatcaatc tgcaatttag ctgatttttc
     5161 ttttttacca tttacagtca cgcgcccagc cttgagcaag tttttgacct cagtccgact
     5221 tcccaccgca caggcaacta aaaatttatc taatctcata gaactattat atcatatcaa
     5281 aaggaggcta gtacaatgac caacctcctt ttcgtttcat actcttcaaa aatctcttca
     5341 aaccgcgtca acgtcgcctt gccgtatata tgttactgac ttcgtcagtt ctatctgcaa
     5401 cctcaaaaca gtgttttgag ctgacttcgt cagttctatc tgcaacctca aagcagtgct
     5461 ttgagcatcc tgcggctagt ttcctagttt gctctttgat tttcattgag tatcagattt
     5521 aggaaattaa cttcctcgtc tccaaaaaat agctaaaaca atcatggctc ctaaaatcgc
     5581 tgggataata gctgttcctg ataaaactgg cccccaagtt ccaaagagca agtgacctag
     5641 aaaggctccg atccaaccga gaaacatttt tccaaaacat cccattcgct ctccacgatt
     5701 ggtcatagca cctgctaaaa atcccactag gagaccaacg aacatacttc ctaacatatt
     5761 atctccttaa tttgcccaat tcccattacg gaaaagaggt acccgcgttc catcctcacg
     5821 aataccatcg atatccattt ggttagaacc aatcataaag tctacgtgaa catctgaacg
     5881 gttaagccct gcagcttcaa gctcctcttc gctcatctcc gctccatcaa caacgctagt
     5941 cgcataggct gcaccgatag ccaagtggtt tgacgcattt tcatcgaaaa gggtgttaaa
     6001 gaaggtaatg cctgactgag aaattggact tggatctggt accaaggcac gcgcacccgc
     6061 attttcaaag acaaggtctt tcataacctg atcacccttc tcagcagtga tatctacgat
     6121 ttgtccatcc ttaaaggtca ccttaatgcc ttcaatgata tttccgttgt agctaagcgg
     6181 ttttgtagaa gtgacataac catctgcacg acggaagtca ggcgctgtga agacctcttc
     6241 tgttggcata tttggcaaga attcttcgcc ctgtgcattg acagcaccag ctgattccca
     6301 aacgtggttc tttggcaaac caagtgttaa atctgttcct ggcgctgtgt agtgaagggc
     6361 tgaaaattgc tccttattaa gcatatcggc cttgctcttg agaatggctg catgttcctc
     6421 ccaagcctta acaggatctg cttcgtagac acggcaagtt ttgaaaattt ggtcccaaag
     6481 gaaatcaact gcttcttcgt cgctcgcagc atttgggaag actttcttag cccactcaag
     6541 tcctgctgca gctgctacag tccagctaac cttgttagat tgagttgcga tacgcatagg
     6601 cttcatggca agtcccatag ctttagcaga agctgaaagc ttgtcagcgt ccactccgtt
     6661 caaggcacct ggatcagatg aacgaactcc aagacggcta gccttattct ccaagagata
     6721 gttcatctca gcaatcttgt attctggcac attgtccaaa cgctccatcg gggcatggag
     6781 gaatttctca cggttaatca catcatctgt ccactgaacg atgacctcat gcgcacccaa
     6841 ggcataagct tctttcacga ttagatgtgc caattcacgt tgctccacat caatagagag
     6901 agccaaagtg tgaccaggtt gcacgttaat tccgttcgca accaacaatt tcgcatattt
     6961 ttctagattt tctttaaaat ttggtaaaac cattctgttt tctcttttct atttttattt
     7021 ttctttcaaa gcagaaaaga ttcctgctaa agcaatagca cctgacaaga gtgctgtcga
     7081 tagaagaatt gtacgatcag caagttctaa ttgatattca aataccttct tagccggctt
     7141 atcttccgca aatattctta gtttcatgag aaccccctta aaatagatta aaataaatca
     7201 ctgtgactgc ctgtcctcag caaatttaaa atcaattccg acttgtctac tttataaacc
     7261 aaaagccaat ctggctgggt atggcattca cgaactcctt gaaaatgctt ggatgccgtc
     7321 aatgaatgat cacgatttct ggcaggatgt tctttttctt gaaccagaaa attcaaaact
     7381 tcttctaata attctgcctt caaaccacgc ttcatagcca acttaaaatc ttttttaaac
     7441 tgtttatgat aacgaatctt aagcacgtaa gtcctccatc aaatctgaga ctgattcaaa
     7501 agactggctc atattacgat tttggtctaa atccgttaac gcttggagca acttccgatt
     7561 ttcgtctagt ctaacatcaa aaggtaatcc ctgatattga attgcctgac gaaggaaaat
     7621 attaatagct gttgtcatat ccattcccag attactaaac acctgttggg cctgctcctt
     7681 aacctcacta tccagacgga tgcttatact catctttgac atactctcac cctctttcca
     7741 tagactattt taacaaaaaa gaaagctaat gtaaatctat tggatatacg ttagcctctt
     7801 ttaatagatt attaagcaat tttttaaaac aactcatcaa acaaactcaa ctggttatcc
     7861 tctggcatat ttccaagaat acccatctca tccatctttt caaccaaggt tgatgagagt
     7921 ccaccacgct tgcgtagttc tgttttagag aggaattctc cctcttcacg cgcccgcacc
     7981 agttgcttgg caacgttctc tcccagacca tccattgcta caaatggtgg gataagggta
     8041 tccccgtcga tgaggaactc tgtcgcctga ctacagtaga gatctaattt accaaacttg
     8101 aaacctcgtt cccacatctc attgacaatc tcaagagttg tatagagatc gatttccaca
     8161 ttagaggctt cattgttctt ccgtttttca gagatttctt ccattctgcg cttgatgacc
     8221 tccaagcccg cacccatggt cttgatatca aaagccttag cacgaatgga gaagtaagca
     8281 cagtagtaat aaataggatg gtgaaccttg aagtaagcta cacgcaaggc catcataacg
     8341 taggctgccg catgggcctt agggaacatg tacttaattt tcccacagga ttcgatatac
     8401 cactctggca ccttattagc cttcattgct tcgatatagc catttctctc ctcttctgaa
     8461 atctttagcc acaaaccctt acgtacccgt tccataatgg taaaggccat cttaggttcc
     8521 agacccgcat gcatgaggta aaccatgatg tcgtcccgac aaccgataac agtcgatagg
     8581 tccgctattc cttgcttaat cagatcctga gcattcccca accaaacatc agtaccgtgg
     8641 gacagaccag acagctgaag caattccgca aaggttgtcg gatgggtttc gtctaccatt
     8701 ccacgtacga aatttgttcc aaactctgga atccccaaca tacccgtagg cgttccaatt
     8761 tgttcaggtg ttacccctag cacatcagtc ccagaaaaga gtgccatcac gccttcgtca
     8821 tccataggaa ttttattagg gtcaatacca gacaaatcct gaagttttcg aatcatagtc
     8881 ggatcatcat gtcccagtac atcgagtttg aggacgttct catcgatatc gtggaagtta
     8941 aagtgagtgg tctgccattc agccgtgaca tcatctgctg gatactggac aggcgtaaaa
     9001 tcgtagacat ccatgtagtt cggaataaca acgattcccc ccgggtgttg gcctgttgtc
     9061 cgcttgacac ccgccgctcc ttgagcgagg cgttctactt ctgcatcacg ataaaacttg
     9121 ccataatctc gctcgtaacc tttgacaaat ccataggcag tcttggcagc taccgtacca
     9181 accgttcccg cacggaaggc atattcttca ccaaagatat cacgcacatc caagtgggcg
     9241 ctaggctgat cttctcccga gaagttcaag tcaatatcag gaaccttatc cccatcaaaa
     9301 ccaaggaagg tctcaaacgg aatatcctgt ccgtttttac tgagtttgtg accacagttt
     9361 ggacagtcct tatggggcat atcaaatcct gaaccgtacg aaccatctgt gataaactca
     9421 ctgtactgac actgaccaca gacatagtga ggagagagag gattgacctc cgtaatccca
     9481 atcatggtcg caacgaaact agatccgaca gacccacgag aaccaaccaa ataaccccgt
     9541 tcattagaac gttgcaccag catctgcgat gccagataaa tcacagcaaa tccattcccc
     9601 agtatggatg ttaattcttt ttcaatccgc aaatcaacaa tatctggcag cggatttcca
     9661 taaatctcaa aagctttctt ataggtcaac tcagcaactg tttcttcagc cttgtcgatg
     9721 aaaggcgtat acaagtcacc cttaacgact tcaacggatt caaatatttc tgccaaggca
     9781 ttggtgtttt caataaccag tttacgagcc agttcctctc ccaaaaaggc aaattcatcc
     9841 aacatctcat tagtcgttcg aaaatgagcc tttggaagtg gtgctggttg ggcatgttca
     9901 ccatgaccga tagttcgatt aatcatcgca ccctgtccca aactacggac gataatttca
     9961 cgataaatct cttcttccgg ttcgatatag tgaacatttc ccgtagccag aacaggcttg
    10021 ccaaggcggt ctccaacctc tatcaaactc ttgataatgg tctggagttc ctccatatcc
    10081 ttgacctgct ctttggcaat caagggtgca tagatagccg gtggcatgac ctcgataaaa
    10141 tcataatact tggccacctc aaccgccgca tccacacctt gagaaacgac cacgtcaaaa
    10201 acttcaccct ctgaacaggc tgaacctaaa atcaagccct ctcgatgggc atctagaacc
    10261 gttctcggaa tccgtggcac tccttcaaaa tacttggtat tagacaagga aaccagctta
    10321 aagatatttt ttagacctac ctgattcttg acatagatgg tcgcatgctt gatccgagct
    10381 tttttgtaag aatctggact gattagatca atgttgagtc tagctaaatc ggtcacacca
    10441 tgtttttctg ctacctcttt gataaagatg aaaagcagac gaccagtcgc ttccgcatcg
    10501 tagttggcca tgtggtgatg ttccaaggcc acaccaaaac gcttggtcaa aggccccaaa
    10561 ccatggcgtt tatactcagg atagaggttt ctagcaaact ccagcgtatc aataactggc
    10621 tgactaatct ttggaagatc atgccgctca taattagcat tcataaagcc aacgtcaaag
    10681 gtagcattgt gggcaactag gaccgtatcc ttgcaaaatt cttggaattc ttgcaaaact
    10741 tgttctagtg gtttggcatt tttgacatga tcatctgtaa ttccagttaa ctctgtagta
    10801 aaggctgaca agggatgccc aggattgata aattcatcaa attcagcaat aacattcccc
    10861 ttgtacatct tagaagccgc aacctgaatc aagtcattat agatagctga aagtcccgtc
    10921 gtttccacgt caaagaccac gtaggttgct tctgacaagt ccatctccac ttcgttatag
    10981 acgataggga cacggtcctc cacgatattg gcttccatcc catagatcag ctggattccc
    11041 gctttcttag ccgccttata gccatgtgga aaggactgga cattcccatg gtctgtgata
    11101 gcaaccgcct tgtgtcccca cttagcagct gttgcaacaa tctcttcgac ctctggcaaa
    11161 gcatccatag tcgacatgtt agtatgagca tgaaactcaa cccgacgctc accttctggc
    11221 atcaaatcct tccgctcata gtgaacaact tcctgcagat cctgtacgtt catagtcaaa
    11281 tcgcgtgtga agttattcat ctccacattc cctcgaactc ggagccaaga attcttcttg
    11341 atgaggtcaa acttctgggc ctcttcctcg tttttaaccc acttttgcat agaaaaactt
    11401 gaagtatagt ccgtcatttt aaagttgatt aaaacacgac ctgttctagt cactttttgc
    11461 tccacatcaa aaacaacccc ttcaaatacc agacgatttt cctctgtcgt cacttcgatc
    11521 ataggagtaa tctccgcctt atccagcttg ggtttagctg cagctttttt cgcttgaaaa
    11581 tcaaagactg gtttctcttc cgctggagga ggtgccatct gctccagttg ttccatagca
    11641 cggagcgctt cctcattggc agcttgaaca atctgctcat tttcagcatg aaaggcctct
    11701 tcctgctctt gggtcaggac atcattcttc tcgacttgac agttaaaagt tggaaaacca
    11761 aacttttcaa gttgtttggc taaattagga agatgattct tcttaaaatg ttccttatca
    11821 atcgcttcag atccttcaat aaatagctga ttaccctcag cacgaacttg caaattttga
    11881 taaagggact taaaaccttg actagcacat ggaccttcag agaaagcctc cctatagtag
    11941 gactgcaaga gctgatttga aaattcttga gaccgagcct taatttcaaa aacagcttta
    12001 ttgcctgtct tagaaaattc ttcgctcaaa cctttcttta attctaaaaa gatttcaatc
    12061 ggtaaaatat tagaaaatac gaaatgaaac tcccatacct tactaatttt atgaaccaca
    12121 actcgctcaa tattggcctg tgctaaagca ggagcctgtc tcatttcagc aggcatcccc
    12181 aattgattca tcaaaatttc aaaactattt gacattcatt ttcctcacat tattcttcta
    12241 ctattttacc atatttagag gtattttcta aagacaaaag gaagccacta agtgacttcc
    12301 ttctagagtg aggacggatt agtcttcacc tttatttttc ttaataattt cttcttgtac
    12361 tgacttaggt acatcttcgt agtggtcaaa taccatcatg aatgtaccac gtccttgaga
    12421 tgcagaacga agaactgttg cgtaaccgaa catttcagca agtggaacgt aagcacgaac
    12481 gatttggctg ttaccgtgtg cttccatacc atctacacgt ccacgacgag cagttacgtg
    12541 acccataaca tcaccaaggt tttcttctgg aacagtgatt gttacaagca tcattggttc
    12601 aaggatagct ggttgtgctg atttagcagc ttctttaagg gaaagtgaag ccgcaatctt
    12661 gaaggcagtt tcagatgagt cgacatcgtg atatgaacca tcataaagct tagctttaac
    12721 gtcaaccatt gggtaacctg caagaacacc gttagccata gattctacca aacctttttc
    12781 aaccgctggg ataaattcac gaggaaccac accaccgacg attgcgtttt cgaattcgaa
    12841 tcctttacct tcttcgtttg gagtaaattc aatccataca tcaccgaatt gacctttacc
    12901 accagactga cgtttgaaga atccacgtgc ttgagtagaa gcgcggaatg tttcacggta
    12961 agatacttga ggagcaccta cgttcgcttc aactttgaac tcacgacgca tacgatcaac
    13021 aaggacgtca aggtgaagtt cacccatacc tgagataact gtttcaccag tttcaacgtt
    13081 tgtttcaacg cggaatgttg gatcttcttc agccaatttt tgaagggcga tacccatctt
    13141 gtcttggtca gctttagatt ttggctcaac catcaattgg ataactggtt ctggaacgtt
    13201 gattgactca aggatgattt tagctttttc atctgtcaat gagtcaccag ttgtagtatc
    13261 tttcaaacca acggcagcag cgatatcacc tgagtaaaca gtgtcgattt cttgacggct
    13321 gttagcgtgc atttgaagga tacgtccgat acgttcacgt ttacctttag aagtattcaa
    13381 tacgtatgaa cctgattgaa gaacacctga gtaaacacgg aagaatgtca aacgacctac
    13441 gaatgggtca gtcatgatct tgaaggcaag agctgcaaat ggctcttcgt cagatgctgg
    13501 acgaatttct tcagcgtctg tatctgggtt aatacctttg attgctggga tgtcaagtgg
    13561 gcttggaagg tagtcgataa ccgcatcaag catcaattga acacctttgt ttttgaaggc
    13621 tgaaccacac aatactggga agaattcaac gttgatagtc gctttacgga taccagcttt
    13681 caattcttcg ttagtgattt cttcaccttc gaggtatttc atcatcaatt cttcgtcagt
    13741 ttcagcaact gcttcaatca atttttcacg gtattcttga gcttggtcaa ggtattcagc
    13801 tgggatgtct tcttcaagga tatccgtacc aaggtcgtta gtatagattt cagctttcat
    13861 cttgatcaag tcaatgatac cacggaagtc atcttcagaa ccgattggca attggattgg
    13921 gtgtgcattt gcttgaagac gatcgtgaag tgtgcttaca gagtaaagga agtcagcacc
    13981 gattttgtcc attttgttgg caaatacgat acgtggaact ccgtactcag ttgcttgacg
    14041 ccaaactgtt tcagtttgag gctcaacacc tgattgtgag tcaagaacgg taaccgcacc
    14101 atccaataca cgaagagaac gttgtacttc gattgtgaag tccacgtgtc ctggtgtgtc
    14161 gatgatgttt acgcggtggt tgttccattg agctgtcgtc gcagcagatg tgatcgtgat
    14221 accacgttct tgctcttgct ccatccagtc catttgtgac gcaccttcgt gagtttcacc
    14281 gattttgtgg attttaccag tgtagtaaag aatacgctca gtagttgttg ttttaccggc
    14341 atcgacgtga gccatgatac cgatattacg agttttttca agtgaaaatt cgcgtgccat
    14401 gaggtttgtt tctcctattt atttttgatt tctattctat tataacacga ttttaataaa
    14461 aacggatagg caggacctac ccgttctcaa tgttttcatg ctattgttgg tttcaactta
    14521 cgagatggta agctgagtta tagctaatac taatcgattt agctaatttg aacccgggct
    14581 aaagttagtt agccgatgtg agctggaacg ggatgctgcg tgaaaaagat aaaactcctt
    14641 gtattcgtcg aatactgcgt cagtttccta tttttacctt gcatccttac cgttcctagc
    14701 atcatgattt agttgagctc aagctaaaag tctggagaaa aagatgaatc tcattggttg
    14761 caaccgcaac ctgcttcgat tccctatttt ctctgggact ttcttaacgc tttcgcatcc
    14821 tatcttacca acggaagtgt gcgaatgcac ggttagcttc agccatacgg tgagtatctt
    14881 cacgtttctt aactgctgca ccagtgttgt tagcagcatc caagatttct tttgcaagac
    14941 ggtcttgcat tgtgtgttca ccacgaagac gagcgattgt taccaaccaa cgaagtccaa
    15001 gtgttgtacg acgttctgga cgaacttcaa ctgggacttg gtagttagaa ccaccaacac
    15061 gacgtgcacg tacttcaagt acaggcatga tgttttccat agctgtttca aatacttcaa
    15121 gtgcatcgtt gccagtagct tctttgattt gctcaaaagc accgtaaacg attgaagcag
    15181 cagtaccacg tttaccatca agcataacgc ggttgataag acgagtaact agttgtgaat
    15241 tgtatagcgg atctggcaat acgtcacgtt ttggagctct atttttacga ctcatttctc
    15301 tttatcccct ttccttatgc ttttggacgt ttagtaccgt atttagaacg gccttgttta
    15361 cgatcgttaa cacctgcagt atcaagtgca ccacggacga tatggtaacg tacccctgga
    15421 aggtctttta cacgtccacc gcgaagaagc accacgctgt gctcttgcaa gttgtgtccg
    15481 atacctggga tgtaggcagt aacttcgata aggttgctca aacgtacacg agcgaattta
    15541 cgaagggctg agttaggttt tttaggtgtc attgttccaa cacgagttgc aacaccacgt
    15601 ttttgtggtg aagaaacgtt tgtttgaact tttttatgac tattgtaacc aacgttcaaa
    15661 gctggtgatt tagatttttc tacttttgat ttacgcggtt tgcgaaccaa ttggttaatt
    15721 gtaggcatct acattctcct gtgttttttt atttttggtg atgatacact tggtgacaga
    15781 tatcatctgt gtgtactttt gcaacatttg tcagcacgtc cctgtacact cttgagagac
    15841 caaaagtaaa aagtaccgtc tattattgta acaaaatttt cccttgcttg tcaagatatt
    15901 tttctttttt attggtaatg ttagagatca atttctgaat aaaaatagct gagaacacat
    15961 catcttccta ataacacggc ttggcttacg tgacattttt atctccgtca tttatactga
    16021 tatataaaat agtacggaaa acttctcaaa agtaagaatt tctttcacaa atcaaagaaa
    16081 acgcttgcat attgttttac tacatgctat actgatatag aaatttattg attggagaat
    16141 cattatgaga aaaacaccat ctcacactga gaaaaaaatg gtctacagca tccgttccct
    16201 caaaaatgga actggttctg tccttatcgg cgcaagcctt gtcttgcttg ccatggctac
    16261 accaactatc tcatccgacg aaagtacacc aaccactaac gaacccaaca acagaaatac
    16321 aaccaccctt gcccaacctc ttactgatac agcagctggc tctggtaaga acgaaagtga
    16381 tatttcttca cctggaaatg caaacgcttc cctagagaaa acagaagaaa aacctgctgc
    16441 aagcccagcc gatccagcac cacaaactgg acaagatcgt tcaagtgagc caactacttc
    16501 tactagtcca gtaacaactg aaactaaggc agaagagccc atcgaagata actacttccg
    16561 tatccatgtc aaaaaacttc ctgaagaaaa caaggatgct caaggactat ggacttggga
    16621 cgatgttgaa aaaccatctg aaaactggcc aaacggagct ttgtccttca aggatgccaa
    16681 gaaagatgac tacggctatt acctagatgt caaattaaag ggagaacaag ccaagaaaat
    16741 tagcttcctc atcaacaata cagctggaaa aaatctaacc ggcgataaat ctgtagaaaa
    16801 actagttcca aaaatgaacg aagcttggtt agaccaagat tacaaggttt tctcttacga
    16861 gccacagcct gcaggaactg ttcgcgtcaa ctactaccgc acagatggca actatgacaa
    16921 gaaatctctc tggtactggg gagatgtgaa aaatccaagt agcgctcaat ggcctgacgg
    16981 aacagacttt acggctacag gcaaatatgg ccgctatatc gacattcctc ttaatgaagc
    17041 cgcaagagaa tttggatttt tattactaga tgagagcaaa caaggagacg acgtgaaaat
    17101 ccgtaaagaa aattataagt tcacagattt gaaaaatcat agccaaattt tcctaaaaga
    17161 cgatgatgaa tcgatttaca caaatccata ctatgtccat gatatccgta tgacaggagc
    17221 ccaacacgta ggcacttcta gcattgaaag tagcttttca acacttgtcg gtgctaaaaa
    17281 agaagatatc ctcaaacact ccaacatcac taatcaccta ggaaacaagg taactattac
    17341 cgatgttgca atcgatgaag ctggtaagaa agtgacctac agcggagatt tctctgacac
    17401 aaaacatcct tatactgtta gctacaattc cgaccaattc actaccaaaa caagctggcg
    17461 cctgaaagat gagacataca gctatgatgg caaactggga gctgacctaa aagaagaagg
    17521 aaaacaagtt gatttgaccc tttggtcacc aagtgctgat aaggtttctg ttgttgtcta
    17581 cgacaagaat gaccctgaca aagtagttgg aactgtcgct cttgaaaaag gggaaagagg
    17641 aacttggaaa caaactctag acagcacaaa caaactcgga atcacagatt tcactggcta
    17701 ctattatcaa taccaaatcg agcgtcaagg taaaactgtt cttgcactcg atccttacgc
    17761 taaatctctt gctgcttgga atagcgacga ttccaagatt gacgatgccc ataaagtggc
    17821 taaagccgcc tttgtagatc cagctaaact cggacctcaa gacttgactt atggtaagat
    17881 tcacaatttc aagactcgtg aagacgccgt tatctacgaa gctcatgtgc gtgatttcac
    17941 ttcagatcct gccattgcaa aagacttgac caaaccattt gggacttttg aagccttcat
    18001 tgaaaaacta gactatctca aagacttggg tgtaacccat atccagctcc ttccagtctt
    18061 gtcttactac tttgtcaatg aattgaaaaa ccatgaacgc ttgtctgact acgcttcaag
    18121 caacagcaac tacaactggg gatatgaccc tcaaaactac ttctccttga ctggtatgta
    18181 ctcaagcgat cctaagaatc cagaaaaacg aatcgcagaa tttaaaaacc tcatcaacga
    18241 aatccacaaa cgtggtatgg gagctatcct agatgtcgtt tataaccaca cagccaaagt
    18301 cgatctcttt gaagatttgg aaccaaacta ctaccacttt atggatgccg atggcacacc
    18361 tcgaactagc tttggtggtg gacgcttggg gacaacccac catatgacca aacggctcct
    18421 aattgactct atcaaatacc tagttgatac ctacaaagtg gatggcttcc gtttcgatat
    18481 gatgggagac catgacgccg cttctatcga agaagcttac aaggctgcac gcgccctcaa
    18541 tccaaacctc atcatgcttg gtgaaggttg gagaacctat gccggtgatg aaaacatgcc
    18601 tactaaagct gctgaccaag attggatgaa acataccgat actgtcgctg tcttttcaga
    18661 tgacatccgt aacaacctca aatctggtta tccaaacgaa ggtcaacctg cctttatcac
    18721 aggtggcaag cgtgatgtca acaccatctt taaaaatctc attgctcaac caactaactt
    18781 tgaagctgac agccctggag atgtcatcca atacatcgca gcccatgata acttgaccct
    18841 ctttgacatc attgcccagt ctatcaaaaa agacccaagc aaggctgaga actatgctga
    18901 aatccaccgt cgtttacgac ttggaaatct catggtcttg acagctcaag gaactccatt
    18961 tatccactcc ggtcaggaat atggacgtac taaacaattc cgtgacccag cctacaagac
    19021 tccagtagca gaggataagg ttccaaacaa atctcacttg ttgcgtgata aggacggcaa
    19081 cccatttgac tatccttact tcatccatga ctcttacgat tctagtgatg cagtcaacaa
    19141 gtttgactgg actaaggcta cagatggtaa agcttatcct gaaaatgtca agagccgtga
    19201 ctatatgaaa ggtttgattg cccttcgtca atctacagat gccttccgac ttaagagtct
    19261 tcaagatatc aaagaccgtg tccacctcat cactgtccca ggccaaaatg gtgtggaaaa
    19321 agaggatgta gtgattggct accaaatcac tgctccaaac ggcgatatct acgcagtctt
    19381 tgtcaatgcg gatgaaaaag ctcgcgaatt taatttggga actgcctttg cacatctaag
    19441 aaatgcggaa gttttggcag atgaaaacca agcaggacca gtcggaattg ccaacccgaa
    19501 aggacttgaa tggactgaaa aaggcttgaa attgaatgcc cttacagcta ctgttcttcg
    19561 agtctctcaa aatggaacta gccatgagtc aactgcagaa gagaaaccag actcaacccc
    19621 ttccaagcct gaacatcaaa atgaagcttc tcaccctgca catcaagacc cagctccaga
    19681 agctagacct gattctacta aaccagatgc caaagtagct gatgcggaaa ataaacctag
    19741 ccaagctaca gctgattcac aagctgaaca accagcacaa gaagcacaag catcatctgt
    19801 aaaagaagcg gttcgaaacg aatcggtaga aaactctagc aaggaaaata tacctgcaac
    19861 cccagataaa caagctgaac ttccaaatac aggaatcaaa aacgaaaaca aactcctatt
    19921 tgcaggaatc agcctccttg cgctccttgg tctcggtttc ttactaaaaa ataaaaaaga
    19981 gaactaaact agccctccta tagaaaaatc ccccaagcat tatagctcgg gggattaatt
    20041 tttgtacaat atttgttgtc ctaataaact tgattaggat tttttattaa gcctctttca
    20101 tagcaaaata agctcgtact ttgggtgcaa cttgtgttcc gaagagttca atagctctca
    20161 gaacctggtc atgaggcata gaaccaagcg gtagatgaag catgaagcgg tccaatccta
    20221 aatcctctat catgcgaatc aatttttcgg ccacctgatc tggattgcca acaaacatgg
    20281 cgccatttgg ccctacctgc tccaaatatt gctcataacg caattcctgc cagtgcggac
    20341 ggtctttgga aatagcatcc accacttgct tagtcggatg gaaataatct ttcaccgcct
    20401 gctcaccatc ttccgcaatc cacccccaag aatgggctcc cactttcaag tctttgtcag
    20461 catggcccgc ttcgcttcca atctcacgat aagcctgaat caacttttta aaataacgtg
    20521 gattaccacc aataatagca tatacaatcg gtagaccagc ctgagcaatc ttcactgttg
    20581 attcgacatg accacctgta gctatccaca agggcaattt gtcctgaact ggacgaggat
    20641 aaacttcttt accagcaatc gtttgagtca atcgaccttg ccagtctaac ttggtctttt
    20701 cattgactaa ctgaagcaag tctaatttct catcaaaaag agagtcgtag tctttcaagt
    20761 cataaccaaa cagagggaaa gattccgtga aagagcccct tccagccata atctccgatc
    20821 gtccatttga caaagcatcg atagtggcat actgttggaa caaacgaatc gggtccatgc
    20881 ttgacagaat gctgactgca ctggtcaaac ggattttctt ggtattgact gccccagcgg
    20941 ccagaacaat ctctggggct gatactgcaa aatccgcccg atggtgctca ccaatcccat
    21001 atacatccaa accaaccttg tcagccagct caatctctgc caccaactgg cgaatgcgtt
    21061 cagcatgact gtaagtttgt ccagtccctt caagctccgt tatttcccca aatgttgaaa
    21121 ttcccaattc taccattgtg attctcctta tctatctctg tacttcaatt tgaaaaatta
    21181 ttctaacacg aatcttgagt acaagcaacc gatttgctca ttagaaaaag cctagataac
    21241 tagacttttt tagcttattc taccgttact gacttggcaa ggttacgtgg tttgtccaca
    21301 tcgaggccac ggtggagggt tgcaaagtaa gcgactaatt gcgttggtac gaccattgaa
    21361 attggtgaga ggtatggatg tacggtcgta aggacgatat cgtcggtatc tttggctaca
    21421 ttctcttctg cgatagtgag gactttggca ccacgggctg cgacctcttg gatatttcca
    21481 cgagtatgat tggcaagaac tggatctgac aagagagcca aaacaggcgt tccttcttca
    21541 atcaaggcaa tggttccgtg cttgagttct cctgcagcaa agccttcaca ctggatataa
    21601 gaaatctctt tgagtttgag acttgcttcc atggctacgt agtaatcttg accacgtccg
    21661 atgtaaaagg cgttacgagt tgtttcaaga agttcacgaa ccttgacttc aatggtttct
    21721 ttctctgaaa gagttgattc gatagactga gctacgattg acaattcatg aaccaggtca
    21781 aaggcttgcg ctttagcatt accatttgct tctccgactg cttttgcaag gaaggcaagg
    21841 gctgcgattt gcgctgtata ggctttagtt gatgccacgg caatttcagg acctgcgtga
    21901 aggagcatgg tatagttggc ttcacgtgag agggttgaac ctggaacgtt tgtcactgtt
    21961 aagcttggaa ttcccatttc attagccttg accaaaactt gacgactatc cgctgtttca
    22021 ccagattggc tgataaagat gaagagtggt ttcttgctga gaagtggcat accgtagccc
    22081 cactcagatg agattccaag ttcaactggt gtatctgtca attcttccaa cattttctta
    22141 gaagcaaatc ctgcatggta agatgttcca gctgcaagga tgtagatgcg gtctgcgtct
    22201 tgaacagcct taatgatatc tgggtctacg acaacttgac cagcctcatc tgtgtaggct
    22261 tggatgagtt tccgcataac agttggttgc tcgtcaattt ccttgagcat gtagtaaggg
    22321 taagttccct taccgatatc tgacaagtca agttcagcag tgtagctagc acgctcacga
    22381 cgatttccat catagtcttg aacttccaca ctatcagcct tgacgattac caactcttgg
    22441 tcatggattt ccatgtattg gttagtttca cgaatcatag ccatggcgtc tgagcagacc
    22501 atgttatagc cttctccaag accaatcaaa agtggtgatt tatttttagc tacgtagatg
    22561 acttcaggat cttgtgagtc aaccaaggca aaggcataag aaccacggat gatgtgaagg
    22621 gcttttttga aggcttcaag aactgagagc ccttcttctt ccgcaaattt tccaatcaaa
    22681 tgaacggcta tttcagtatc tgtctgcccc ttgaagtggt gacctgcaag gtattcttcc
    22741 ttgatttcaa gatagttctc aatcacccca ttatgcacca agacaaaacg ttccgtctca
    22801 gagcggtgtg ggtgagcatt gtcctcagtt ggttttccgt gagtagccca acgagtatgt
    22861 ccgataccag ttgttccctc aacaccagct gtcttggcag acaattctgc aatacgacca
    22921 accgccttca ccaaatggtt atcagcacca tctaggacaa aaattcccgc agaatcatag
    22981 ccacggtatt caagcttttc aagcccttga atcaaaatat cagttgcatt tgtgtttcca
    23041 acaacaccaa caattccaca catagtatat acgacacagg caagctgtgc tttctcctta
    23101 aaattggtat agtctaattc atcttttata gaatcagcaa aaacagtata tacttgtttc
    23161 tttcacttgt caagagtaaa aattggtata gttcaaatta agctcctgta agcataaaaa
    23221 ctctgaccga ttgggataat cagtcagagt cctttttaaa atccattatt atcgcttaat
    23281 tctttgaacc agtggcctga tttcttcaga cgacgttctt gcgtttccaa gtctaattcg
    23341 accaaaccat agcgattttt atagctgttg agccatgacc agcagtcaat aaaggtccaa
    23401 atcaagtagc ccttacagtt ggcaccatct tcaatggcac ggtgaagttc acgaagatga
    23461 ccttttacaa agtcaatacg gtaatcatct tgaatcattc catcttgacg gaatttttct
    23521 tccccttcaa cacccatacc attctcagtc aacatccact caatattgcc ataattttcc
    23581 ttgatatttt gggcgatgtc ataaatccct tgctcataaa tctcccaacc acggtgagaa
    23641 ttgattttac gtccaggcat cacataaggc tcgtaaaaat gttctggtaa gagtggactc
    23701 tctggatgct tagcaaatcg aggagccata acacgcaaag gttgatagta gttcacacca
    23761 aggaagtcca ccgtattatc acgaatgagt tccaactctt cctctgtagc atcaggtaaa
    23821 agaccgtgtt catgcaagat ttctaccaac tcctgtggat aagtccccaa gacagatgga
    23881 tctaagaaag attgggcctg aaaaagggcc gcaatacgag ctgccttgac atcagcagga
    23941 tgctggctac gtggataagc cggtgtcaag ttgaggacaa tcccaatctt ggaatcaggc
    24001 aaaagttcat ggcaagcctt aacagcccgg ctgctggcca attgtgtatg ataggctacc
    24061 ttaacagctg cctctgcatc caccttatgt ggataatggg catcataaaa ataaccaaat
    24121 tctacaggaa cgatgggctc gttaaaggta atccattgat ccactaaatc tccataagtc
    24181 tcaaaacaaa aacgagcata gtcttcatag gctgagactg tcgccttatt ttcccaacca
    24241 tcaccatcct cttgaagggc aaaaggtaaa tcaaaatgat agagattgac taacagacga
    24301 attcctttag ccttaatagc ctcaaagacc ttacgataaa aatccacacc ttgagtgttg
    24361 acttttccac agccttgtgg aaaaatccgt gaccactgaa tagaagtccg aaaggctgtg
    24421 tgaccagtct ctaacaaaag ctcaatatcc cgctcccaat tttcataaaa agtcgatgtc
    24481 ttatctgaac caatcccatt atagtaacga tttggctcca cttggaacca gtaatcccag
    24541 agattgtctc ccttaccgtc accagctaca cgtccttctg tctgcggtcc agaagtagag
    24601 gatccccaga caaaatcctt tggaaatctt agcatacatt tacctcttta tctactcatt
    24661 tctcccatta tacagaaaaa acaaggtaaa aactagttac attttttcct tgtttttctt
    24721 ctgattatag tttttatttc ttgcttagga tttcaagcgt ttcaagcacg ttatctgcat
    24781 gaacctcaat ggtgtcacca gttgccttga tcttaacttc tacaatgcca tcggccgctt
    24841 ttttaccaac agtgatacgg attggaagac caatcaagtc actatcgcta aatttaacac
    24901 cgacacgttc gttacggtca tctgtcaaga cttcataacc agctcccatc aagcttgctt
    24961 caagtttttc tgtcaaggct tgcgcttctt catccttgac attgacagta atcaaatgca
    25021 catcaaatgg tgccaattct ttagggaaat tgattcccca agcgtaacgg tattcacctt
    25081 ttggcgtttt gttaacaaag aggcgagcgt gttgctccat cactgctgaa agaagacggc
    25141 tgacaccgat accgtaacat cccatgatga ttggcacagc acgaccattt tcatccaaga
    25201 catctgctcc catgcttgct gaatagcgag ttccgagttt gaaaatatga ccgatctcaa
    25261 taccacgcgc aaagttaagg acaccttgtc catctgggga aatttcaccc tcacgaactt
    25321 cacggatatc cacatattct gcagtaaaat cacggcctgg gttcacacca gtcaagtggt
    25381 agtcatcttc gttagcaccg acaactgcat tgcgaacatc ttgtacctta cgatctgcaa
    25441 taattttaat attctctggc aaaccaactg gtccaagtga accaaatcct gcttgaacaa
    25501 cattcgccac ttcttcttcg ctagcaacgt caaagaaatc tgctcccaag tgatttttca
    25561 acttgacttc gttgagttgg tcatttccaa ctagaagggc tgcaacaagc tcaccatctg
    25621 caatgtagaa gagggtttta atcgtttgtt cttctggaac attgaggaag gctgcaactt
    25681 catcaattga tttaacatct ggcgttgcaa cacgagtaac ttcttcttca gcgacaacac
    25741 ggttgcttgg tttgtactcg tttgttgcca tttctaagtt agctgcatag ctagactcac
    25801 ttgagtaagc aatggtatct tcaccagaga ctatccattt gagcaattct gccttgattt
    25861 cttcttgcac ttctgcagga atttcgtcaa atgaggcaac tgacttgtcc aagacaaccc
    25921 agcggtcaag gtctgtacga gcagatgtaa tggccataaa ttcttggcta tccttaccac
    25981 ccatggctcc accgtcacca ataatagcct tgaagtctaa accactacga gtgaaaatac
    26041 gctcataggc tgctttgtac tcatcataaa cactatccaa actatcatag ttagcgtgga
    26101 aactataagc atccttcatg ataaactcac gtgtacgaag aagtccatta cgcgggcgtt
    26161 tttcatcacg atacttgggc tgaatttgat aaaggttgag tggcaattgc ttgtaagatt
    26221 taacagaatc acggacaata gctgtaaagg tttcttcgtg agttggacct aagataaagt
    26281 ctgatttttc acggtttttt agtttgtaaa ggtcttcacc ataggtttcg taacgacctg
    26341 attcacgcca caattctgca ctaagaaggg ctggagccaa catctcaaca gcaccaatct
    26401 tttcgaattc ttggcgcatg atgtttttag ctttttcaat cacacggttg gcaagtggta
    26461 gataagaata aacacctgct gaaacttggc gaacataacc agcacgcaac ataagagcat
    26521 ggctgataac ttgagcatcg cttggcattt cgcgaagcgt tgggataggc attttacttt
    26581 gtttcataat attcctcgat tatctaaaaa agagtcgcat aatgtcattc caagtcacag
    26641 caatcatcaa gacaaccatg atgaccactc cggccaaggt gacataggtt tcaatttctt
    26701 gtttcaatgg tttgcggcgg atggcttcta ggatattgag cacaatctta ccaccatcca
    26761 aggctggaat cggaataaga ttaaaaatcc caatattgat ggaaatcatt gccaagaagt
    26821 acaagatatt ttcaattcca tttttagcag catcactact tgccttaaag atagcaacag
    26881 gtccacccaa cttgttcaaa tctggttgga aaatcagatt tttcagagct gagagaattc
    26941 ggagagctga gtcagcagca gttgtaaaac cacctacaaa catggataga aaatctgact
    27001 taacccccgg ttgaacacct agaaggtaac gaccttgact atctttgggt gtaacagtga
    27061 cttgtttgtc actccccttt tcagaaatag tcacatccaa agtcggtgcc gtcttatctt
    27121 tggtttctgt ttccacagct tggatcaagc tttcccagtt gctaacctca tgtgagccaa
    27181 tcttggtaat ttgtgccatt tctggtactc ctaccttggc caaggcacct tggggcatga
    27241 tatggaactg attggtatca acatctctga caccaccctg cataaagatt aaaacccaaa
    27301 aaacaacgac acctaagata aaattgttca taggacctgc aaaattggta atcagtttgc
    27361 cccagatagt cgcattttga tattgaacat ctaaaggtgc aatccgaacc tcagtaccat
    27421 ctgcttccac aaccgttgca tcgtgatcca ctgcaaatgt tttttcttct tccagaacca
    27481 atcctttgat aaagagcttg tcttcaaaat caaactgggt cacctgcata gggagggctg
    27541 tttgatccaa ttttttacct gagagattga tgcgtttaac cttaccatca tcagcaagtg
    27601 tcaaactaac aggcgttcct gtcttgattt cagttgtatc atcaccccaa ccggccatgc
    27661 ggacatagcc acccagaggc aagattcgaa tggtataggc cgttccatcc ttgccaatgt
    27721 gagcaaaaat tttaggtccc ataccgatgg caaattcacg tactaaaatc cctgatttct
    27781 tggcaaagta gaagtgaccg aactcgtgca ccactacaat aatcccgaaa accagaataa
    27841 aggttaaaat tccgagcata gcgtttcctc cgtcttttga ttaaaagagt ccaaataagt
    27901 gcatgattgg aaatacaagc aacatactat cgaaacgatc caaaacacca ccatgtccag
    27961 ggataaattt cccagaatcc ttaacaccaa aatgacgttt gatcgaactt tctagtaaat
    28021 caccaaattg tccagcaatg ctaaagaaaa tagcaaagac tgacatcttg taaattccat
    28081 atggaagagc aactgtactg tcaactatca taaggataat ggttactaaa attgctccta
    28141 aaataccacc caaggcaccc tcaagggttt tattaggcga tacccttggt gctaactttc
    28201 gtttcccata gttcatccca acaagatagg caccactgtc tgtcgcccag acgatacaca
    28261 aggctaagag agccttgtcc aaacctgcaa cacgagcatc tagtaaagca ttaaatccaa
    28321 agcccacgta gaagctcata gcaagaggga aaaccgcatc ctcaatcgta taagacttgc
    28381 taaaaacggt cgttcctaac atgattgaaa tcaaaacact ataggcaacc acattcccat
    28441 caactggcaa aaaagtcagg taattctcca agggaatggt caatgcaaag gttgcaaaga
    28501 gggtcaagag gccctccatc gtcatggtct ctagacctct catcttcaaa agttcatgca
    28561 tggctagcat ggctatgatt ccgattgcta tctgaagcaa gaggccccca atcattaaaa
    28621 ttggtaggaa aatagccagg gcaatccctg caaacaaggt tcttttctgt aaatcctggg
    28681 tcatatttcc tcctaaactc ctccaaatcg gcgatgacga cgattatagg caagaatagc
    28741 ttcctgcaag gccgcttcgt caaaatcagg ccataaggtg tccgtaaaat aaagctcact
    28801 ataggctccc tgccatggaa ggaaattgct caaacgtaat tctccactag tacggataat
    28861 caagtctggg tctcgtaagt ccttaggcaa atgctgagta aagagatagt taccaatcaa
    28921 ttcctctgtg atgtcacctg ggttgatttt ggcatctaaa acatcctggg aaatcaactt
    28981 aagcgcctgt gtaatctcag cacgtccacc atagttaaga gcaaaattaa gaatcaatcc
    29041 tgtgttgttc ttagtcaatt cctcagcctt ggttaaagct tcaaaggttt gcttaggcag
    29101 gcggtctgtc tccccaatca tttgaatctt aacattattc gcatgtagtt ccgggacata
    29161 attatcataa aactctactg gcaagttcat gataaacttg acttcctgat ctggacgggt
    29221 ccagttttcc gtagaaaaag catagaccgt aataaccttg acgcccagtt tgttggctgc
    29281 cttggtcacg gtttgcaatg cttccatgcc cgccttatgt ccaaaaactc gcggttgcat
    29341 acgtttttta gcccaacggc cattgccatc catgatgatg ccgatatgag caggaacctg
    29401 tgtcggaacc tctacttcca cagccttatc tttcttaaaa aatccaaaca tgatcttatt
    29461 cctattcaaa aatctatcgt ttcattatac catatttccc cattttcttc tatcactaag
    29521 ctatttattc tcaggcacca agcccatttt tcaaaaaaat aagccgcctg attgggcgac
    29581 tttattttta tagggagatt attatgaaaa agttttagga gtttaagtta aggtcttctt
    29641 aacttatgaa cttagtgtac actccctagc ttaaagtttc cttaagtatt tttaaaaatc
    29701 aaatttttcc atttctcctg ccaatttttc ttggataaac gtgtttgata gagttccatt
    29761 cggtcttcat tttctaagaa atgaggagtt ggacgaactt gaaaattcaa aatatcctcc
    29821 aaaccataag gtacatagag ttcaaaatct aattcttcat tcaagcgcag tccaactgcc
    29881 gtacaccgtt ctggatactt actcatagca tcacgagaac tggtatagga agcagtgtga
    29941 ggactgtgct gatgcatata gacctgattt ttcaattccc actggtactg aggaaaatcc
    30001 tctctcagct ttttctccag taataaggtt tcctcataag aaaaatctgg atcaaagaaa
    30061 atcacatcta tatctgtttc atgatcaaaa ggggatttgt ctgacaaaag attccagatg
    30121 aaatttctga cagaacctgc tgccaaccac gagtctttca aaccaaggtc tcggatgatc
    30181 gtcagaatgg ccatcatatc tggactttct ctaaaagcct ctaagatttc ttgcttattt
    30241 ttcactgtat tcataaccta agtgctcata tgccttagca gtcgccaccc gtccagaccg
    30301 tgtccgcatg ataaaacctt tttgaatcaa gtaaggctca tacatgtctt caactgtctc
    30361 acgctcttcg gcgatattca cagaaagagt tcctagacca acaggtcctc cactgtacat
    30421 ctcaatcatg gtgcgaagga ttttttgatc cacatagtcc aaaccttcat ggtcaacatc
    30481 cagcatagtc aaagccttat cggtaataac atcatcgata accccattcc ccattatctg
    30541 ggcaaaatcg cgcacgcgct tgaggagacg attggcaata cgaggggttc cacgactacg
    30601 tagggccaac tcagatgctg cctcatgggt gatttccatc tcaaaaatat ctgccgtccg
    30661 ctcgacaatt tctgtcaagt cagcatgagc ataatactcc atatgacctg taatcccaaa
    30721 acgtgcccgt agtggatttg agagcatacc agcccgagtc gtcgcaccaa tcaaggtaaa
    30781 aggaggcaac tccaaatgaa cactgcgact gccttcacca gccccaatca taatatcgat
    30841 gtagaagtcc tccatggcac tataaagcac ttcttccact gacatgggta agcgatgaat
    30901 ctcgtcaata aagaggacat ctccaggctc taaatcattc aaaatcgcta ccaaatcacc
    30961 cgctttttcg ataacaggac cagacgtttg cttgagattg actcccagtt cattggcaat
    31021 gacaaaagcc atggttgttt tcccaagccc tggagggcca aataagagca catgatccag
    31081 cgcttcatcc cgcattttag cggcttcgat aaagatctga agttgatcct taaccttatc
    31141 ctgaccaata tattcacgta aatactgagg acggagcgtg cgttctacta actcctcatc
    31201 acccatcatc tcattatcta aaattctact catggctcta ttatatcaaa aaaaacaagc
    31261 cacaaacaaa aaagccacct gattgggtga ctcctaagtt tagcacttat gtggtataat
    31321 attatacggc acttctacac cgcctacgaa aggaggtgag atagcccatg atggaattag
    31381 tactcaaaac tattatcgga ccaattgtgg tcggtgtcgt tcttcgtata gtcgataaat
    31441 ggctaaacaa ggacaaatag tgtcaaaaaa gacctcaagc ttatttggtc gtgagcttgg
    31501 ggtcttttct agcctatgat atagaactag tactcaattc ctttttatta tcccatagtt
    31561 cacgaatttt gtcaaaactt tacattttct tcaaccgctg tacgacaaga cggttaagat
    31621 taagagaacg ttagggattc tatcaatttc atagaaattt tgatttcgta aacgaagaga
    31681 caatcttaca tgtcacttct catttaatac gccactacta gacaagcaaa atcattatta
    31741 cagtagttcc agtccttcaa ttaacagtca cttacaatca aattgagttt gaactagctg
    31801 aagcgaccac agacctattt cttagtcata ttcgctaaaa aaatccccgc caaaatctca
    31861 aaaagtcccc gccaattccc cgaccaaaat ccgaaaaata ccgaaaaata tcgaaaaatt
    31921 atttttagaa tagtcccaaa aatcctgaaa tagagctaaa aaactccacc tgattcggtg
    31981 gagttaaggg agattattat gaaaaagaaa agtttaggat tttattaaat aaagttagga
    32041 ggtctttatt taataactac atgatacaag acgaaactta aaactagctt aacttttcta
    32101 aaattttact attttgcaaa aaatttctat caccagcacc tcaccaatcg agtaggggat
    32161 aatctctagc ccctctcaca ccaccgtacg tgccgtttgg catacggcgg ttcaactaac
    32221 ttttaacgca tgtcgttcaa ggtaataatc caaacacgaa accagtccac gtttttccag
    32281 gactggtttt gatatagcac gtttaagtac cgacttctga gctactaatt gataatggtc
    32341 gccccagcca gataccttat ctgctatcca tttaggaact cctaacttaa gcaatcccca
    32401 taatcgtctc gatttcttct tccattgctt ccagataatc actcgtaggc gagtacgcaa
    32461 gcgctcatct atgctggcga ctatactttt catatttccc aatgagcaat agtttatcca
    32521 tcctcgaata gacaaattca gttgctcaat acgtcttgtt aggtctatac tccatttcct
    32581 ctgtgttagt ttcttcaatt taaacttaaa tctccgaaca ctatcttgat gtggacggct
    32641 tttccaacca tctgataatt tccagaaccc aaaacctaga tatttcaact ctcttggtca
    32701 tgtttacttt caaacctagc cgtttctcaa taaaacgact gactgaatac atcacacgct
    32761 tagaggctgc ctcgcttccg accgtaatca cacaatcatc tgcgtagcgc acaaatcgaa
    32821 gtcccctctt ttctaattcc ttgtccaatt cattaagcat gatattggat aagagaggag
    32881 ataaatttcc tccctgtggt gtaccaacta gtgttttata acgttgacca ttaatgataa
    32941 cacctgaatg aagatactta cgaatcaagg attccgtatc tccgtcttcg ataatgttat
    33001 gtactaagga catcaatcta tcttgaggaa ctgtatcgaa aaatttctct aggtctaatc
    33061 cactatccac tcatagccgt catttaagta ttctaagagc ttcatgatgg ctttttcaca
    33121 tgacctattt ggtctgaaac cataactcgt atctgagaaa tggggttcac aaatggggct
    33181 catgacttgg acaatggcct gttgaatcat tctatccata actgttggaa ttcctagttg
    33241 acggatgcct ccgtctggtt taggtatctc aactctaaga actggttgag gcttatattt
    33301 tctctgtttt atcagttcct tagtcaagcg ccagttttgt ctgagataat tatccatctc
    33361 ttcgatagtc attccatcaa tcccagctga gcctttattg gattttactt gattgtaggc
    33421 ttccagcata ttttcgcgtg ataatatctt atctagcagt tttgacatgt gcgtattcct
    33481 ttcttatttt ggtgcctgag ggacatctta tattggtgaa ccttcatcta gtcattacgt
    33541 gtcaaagtcc agttctatca gaccactcat tttacagaca caagtgaagt aggattactt
    33601 cgatctgtcg ttttctcttt ctagcgacag acctcaaaca gtctctcccc agactgtttg
    33661 agtattcacc ccttcgctcc acttccatta cggaagtttc ctcactacta tgggctcggc
    33721 tgacttctca tgattcgttg ttactacgct ttcagcgctc atgaaacctc acgggataag
    33781 tcgtacatct ttcctcgttt atacaagata tgaggacgtt aaaaggttac agtgaaaata
    33841 ggaaatctga cgcagagtct ttagactcta gaaagattta tctttttcac acaaacctta
    33901 gtccgaattc aattactctc gtgatttaca catataggtt acgactacct ttttggactt
    33961 tagagtttga agcctcctta tccgctatat acgccttact atcacgttcc tgttcgtaga
    34021 gccacgattt cgctatccct tcctctccct gctacctcac gatagtcagg ctgaggactt
    34081 acacctcaga tatacgacat gcccgtcgta ccgcaaaaaa gccacctgat tgggtggctt
    34141 cattaggaga ttgtgatgaa aaaagtttag gattttatta aataaagtta ggaggtcttt
    34201 atttaatgac tatatgatac tagaccatcc ttaaactttg cttaagaaca aacaagtttt
    34261 tttatttttc tctgtttatc caaatcattc ctgtacagtc ataggtggat aagatttcaa
    34321 agcccataga acgataaaat cccacgtttt tttctgtctc ttctgtcgcc agctggactt
    34381 gataggcctc tttaaaattt cctaaagcct ctttcatcaa ggagctacca atcccttgac
    34441 gctgatagct aggcaaaaca atcaaatcct gtacaaaaac tgatgaaaaa ccatctccaa
    34501 ccaaacgaat caagcccacc acagcatcac catcaagtgc cagataaatt actaatgaat
    34561 gagataaggc ctgctccagc atctctgttt gatgggtata gtttgtccaa ccgacagcct
    34621 gatagagatg caaaacatcc tctagcttga caatttcttg ctttttaata gtaatcatct
    34681 caacacctct taaagttctc tcaagctctt gtgctgtcgt ccatttttat caaagttttc
    34741 aggacgcaac catatttcca atcgagcttt gacttgaggc cagtccttat caatcataga
    34801 caaccaatcc gtatctcttg tacgcccctt ataaaccact gcctgacgga aggttccttc
    34861 ataaataaat cccaaacgtt ccgctgctcg tctggatggc aggttaagag catcgcattt
    34921 ccactcatag cgacgatagt taagctcctc aaagacatag caagccaaga gatactgggc
    34981 ttctgttcct atccgtgtcc ccctgagctc tggagaaaaa gtgacagctc ccacttctat
    35041 tactcggtta ttctgatcaa ttcgcatgag ggaaaaagtt cccaaagcct taccagttgc
    35101 cttgtctatg attgcataat aaaaacggtc cttacgagcc aacatctgat ttaaaaggct
    35161 gaccagttcc tccatgtctg ctactgactc ctgaaagagg taggtccaca tctcccgagg
    35221 cgtatcaggg ccataaacag ctaataaatc ctccgcatgc ttttccactg aaagagcctc
    35281 tatcctagca taacgccctt ctaagaaatc aaaagaaggc aattcacctg gagtataagc
    35341 ttccattgac tccccaatca tttgaccata ttcatttact ggcatcttat tctccttatt
    35401 tcacttttca aactttatag ttgcccatag tatatcatac tttcataaga agattcaaaa
    35461 aatcctccag attctactct gaaggactcc tatcttattt caaaacaata ttaacgagtt
    35521 taactggctc aaacagttta ggaaactgtc cgagatttga aaccagcaaa taaattggtt
    35581 atttcacaac aatattaacc aatttattcg gtactgcaat cactttcacg atttccttac
    35641 cgtcaatttc tgctttgact ttttcatccg ctagagaaat ttcttgcaat tcttcacgtg
    35701 ataggtcttt agcgaccatg agtttggcac ggactttacc tttgatttgg acaacgattt
    35761 cgatttcgtc ttcaaccagt ttactttcgt cccaagttgg ccaagctaca taagagattg
    35821 actcacctgt ttctgcgact gtttgccaga gttcttctgc caagtgaggt gcaaatggtg
    35881 caatcaattg aataaagcct ttggcatagt caacataaag cttatcttcc ttgttagcag
    35941 cattgacaaa gaccataagt tgggcaatag ctgtgttgaa tttgagagac tcaatttgct
    36001 cagtaacagc tttgactgtt tcgttgtaag ccttgtcaag agcaccattg ttttccgcaa
    36061 ggatttcttt acttgtaatc aaacggtaaa ctcggtcaag gaacttacgg cttccttcca
    36121 aaccttcttc tgaccaagca atcgaagcat cgagtggtcc cataaacatt tcataaacac
    36181 gaagggtatc ggcaccgtat tgttccacca catcgtctgg gttaacaacg ttcttgagcg
    36241 atttagacat cttggctggc gcttgctcca actcttcccc tgtttctaca tggaagaagg
    36301 aaccatcacg tttttcaacc ttgtcggttg ccacaagagc accacggtgg tcacggtagc
    36361 ttgttcccaa aatcatccct tggttaaaga gtttttggaa tggttcctta gtcggaacaa
    36421 caccgaggtc atagaggaat ttatgccaga aacgagcata aagcaagtga agtacagcat
    36481 gttccgcacc acccacgtag atatctactg gcaaccattg tttgaggagg tcctcatcag
    36541 ccaatttctc agtattgtgc gggtcaatat agcggaggta gtaccagctt gaaccagccc
    36601 attgtggcat agtgttggtt tcacgacgac ctttgacacc atcttcacga gtcacttcaa
    36661 gccaatctgt caagttagct agtggacttt caccagtacc tgaaggacgg atatccttgg
    36721 ttacaggcaa gacaagcggc aattcagttt caggaacagc tgttgaagtt ccatcttccc
    36781 aatgaatgat tggaattggc tcaccccagt aacgttgacg gctaaagagc cagtcgcgga
    36841 gacggtaggt aaccttctcc tgaccacagc ctttttcttc caaccaagcc acaatcttgg
    36901 caatagcgtc ttctttgttg agtccatcta ggaagtctga attgacatgc aggccatcct
    36961 ctgtgtaggc agcttcttcg acatttccac cttcaagtac ttcgacgatt ggaaggtcaa
    37021 attgtttggc aaattcccag tcacgttggt cgtgggcagg cacagccata accgcacctg
    37081 ttccataact agcaaggaca tagtctgcaa tccagattgg catttcctta ccattgacag
    37141 ggttgatggc ataagcacca gtccaaaccc ctgttttttc tttagcaagg tctgtacgag
    37201 ccaagtcaga cttaaggctg gcttggtgtt tatagtctgc tacagcttct gcttgctctg
    37261 aacttgtgat agcgtctact aattcatgtt caggagccaa gacaatgaaa gtcgcaccga
    37321 aaagtgtgtc cggacgagta gtaaagactg taaattcctt gtctgttcct tttactttga
    37381 aagttacatt ggcaccagtt gatttaccaa tccagttgcg ttgcatatcc ttgatagact
    37441 ctgaccaatc tagttcatct aagtcattga gcaagcgctc tgcgtaagcc gtgattttga
    37501 gcatccattg gcgcattggt ttgcggacaa ctggatagcc tccacgctca gaagttccgt
    37561 caggaagcac ttcttcattg gcaatggcag ttcccaattc ctcaacccag tttactggca
    37621 cttcagcttc ataggccaag cctttttcgt aaagcttggt gaaaatccat tgagtccact
    37681 tgtagtagtt tggatctgtt gtgttgactt cacgatccca gtcataagaa aatccaagcg
    37741 cattaatttg acgtttgaag ttggcaatgt tttccgctgt aaattctgct gggtcattac
    37801 cagtatccat agcgtattgc tctgcaggca aaccaaaagc atcccaaccc attgggtgaa
    37861 ggacattgta gccttgcgca cgtttgtaac gactgaggat atcggttgca gtataacctt
    37921 ctgggtgtcc tacgtgcaga ccagctccag acggataagg gaacatatca agcgcataaa
    37981 acttaggttt tgatgcatct gttcctgtct taaatgtatg atgttctgcc cagtagccct
    38041 gccacttagg ctcaatttct ttatgattgt aaaaactcat ggtctctctc caattttgtg
    38101 atgttactat tataccattt ttgagggaat ttgaaatgtg tttaaaggaa aaactgtata
    38161 ctgtatctca ttaattttga tgactatgaa ttgctctatc tcccactttt tatcccattg
    38221 ttatgcagta aatcaaggag taagtcaaga agcttatctc gtatagcaca aaatacttat
    38281 tttagaccga agataaaact ggaatgctaa ttaatgtatt cacaataaat agccgacaac
    38341 ctatgtcgct ttttgattta tgaagaacat atagtagact gaatctgaaa tagtacgaaa
    38401 caattgctaa aacatttata gaaattaatt ttactttccc aatcgatttg ttctcatcct
    38461 atttcaatct tctatagaat tcaatacagg gatcaataaa agactaccat ttggaaaaca
    38521 gtagtcctta ttgtttattt tgaatttaca taggcatcta cagcgataat agcttgagca
    38581 acatctgaag gcgaaatctt aaacggcatc tgatgaattg tctcaccctc catagttgct
    38641 tgtttaccaa cttttattaa atcatcatat ccaacttgat ccaaatgcat ttcttttaga
    38701 gttgttggca taccaatttt tttgtaaaac tcaatatact tatcaagttc ttctttaggt
    38761 ctattttcca ataatagttg tactaaagtt ccataagcta ctttttcacc atgtgttaaa
    38821 tgatgaatgt cacctgtcaa tgcagtaaaa ccattatgaa ttgcatgcgc cgcagctaat
    38881 cctccacttt caaaacctag accactcaat aaagtattag cttcaacaat attttctaat
    38941 gctggtgtca ccactttagc ttcacaagct gccatagcct gtaaaccatc tgcaaacagc
    39001 gtttcttcac atttcttcgc aattgcaact ccagccaatg tttgctgttg tcccaacata
    39061 gtttttccat ttgcctgcat aaccgcacgc gcctcaaccc aagttgctaa accatctgca
    39121 ataccagacg ctaataaacg cttaggggct tgtgaaataa cttttgtatc aaccaaaact
    39181 aaatctggat ttttagaata aaatagataa tgatcaaatg caccttcatc tgtataaata
    39241 acagataaag cagatacagg tgcgtcggtc gatgcaattg ttggagcaat aataacaggc
    39301 ttttcaatca aatctgcaat agcttttgcg ctatcaatcg tctttccccc accaagaccg
    39361 ataatactat cacaattttc tttctcagcc aaggcaacaa ctcgattgat ttcattgtca
    39421 gaagcttcac cattaaatag cgccagaaca atatggaaac catacctatg taggtaatct
    39481 tcaaatcgtt ttccaacaat atcataaacc aactgatcgc ataatagaat agggcaattt
    39541 cccaaatcca aaattgattt ggcattttca aacaaggcat tttccccctg aatatatcta
    39601 gaaggactag caaaaattct catatgtatt tcctctctat ctaaatggaa cgactatttt
    39661 gaataacaaa ccaatcgtca gaaaaatcat caaccgcctt ttggatagat ggcatggcga
    39721 aagctgattc aaaaacatcc gctcctgctg taacagcatg cgcacctgca gctaaagcat
    39781 tatttacttg tgctacattt ttaaaggatg cagctaaaat cttactagga gagttctgtc
    39841 tatcaatagc aagagctaat tgacgaatga cagaatttga atcaatgttc agattttcca
    39901 ttctattata atatggagct aggtaatccg ctcctgcttc gatagctaat aatccctgaa
    39961 taactgtata aatagctgtt gcagtgatat ggtagccctc tttttttagc gcctttattg
    40021 cacgtaatcc agctggagta acaggtactt tgataaatat atcatctcct gcttgtcttc
    40081 gaattttatg agcatccttt aagatgcctt caaaatcttg agaaatcacc tgaacatgaa
    40141 tagagggtgt agagccaatc aattctctta catctttgat tcgttcaaaa aaattaatag
    40201 aaccctctct ttttgcaata gtgggatttg aagttacccc agctagcggc aaaatttcag
    40261 accacttttt aatctcatct aaatttaatg tgtcaagcat aaattccata aaaagaacct
    40321 ctttatttta caaagtatgc tcagtacgtc ctataatgtc atcttgggtt gccttagaaa
    40381 gaacattgaa gaatgcagag tatcctgcaa cacgaacaat taagtctctg tgtttttcag
    40441 gatgtttctg agcgtcaatc agcgtctctc tggaaacaac attgtattga atatggtacc
    40501 catgtaaacg attaaagaat gttcgtaaca aagcaattag ttttaattta tcttcttctt
    40561 tggctaacgt ttgaggattt actttctgat ttaagagaac cccacctacg atttcatctg
    40621 ttggtaattt tgaaacagat tttaaaacag atgtagggcc gtgttgatcc atattatgtg
    40681 atggtgaaca accctctgct aacggtgtac ccgcgttgcg tccatctgga gttgctaatg
    40741 ttccacgtcc ctgccctacg ttggctgaga tagaagatgt tcctgaataa cgaattcctc
    40801 caattggacc tcttccataa cgtgtattag gatatttagc aatttcatca acataaatgt
    40861 cataagcagc agtaaccaat ttgtcagcat aatcatcatc attaccatac ttaggtgcat
    40921 catgaatcaa catttcttga atgaccttac cttcttctcc ggcataatct gtttccagtg
    40981 catgccaaag ctgacttggg cttatacgtt cttcctcaaa caccaatttt ttaattgcag
    41041 ctaatgaatc cgacaaattt gcaattccaa cttgcaatcc tgatatataa tcatatactg
    41101 ctccaccttc tttaaggtgt tttccacgac caatacaatc atcagtcaat gctgaacata
    41161 gaatatcagg aacttctcgt tccaatgata aatcaataga attttcaaca ataacactca
    41221 ttcgtgtcaa atatcttagt gttttatccc aagcattttc taattcagaa aagttcttca
    41281 tatccttaaa atgaccaaag cttggtgcaa accgtttacc cgaagccgga tcaattccat
    41341 cattcatcgt gataagtaga accttaggga agttcatata actcatacct gtgcaacgat
    41401 agccccattt ccctggaact gccgtttcaa cacatccaat ggcactgtaa tcataagcat
    41461 catcttccaa tactcctttt gcaataaaag aaggaataat aatctcatca ttattaaatg
    41521 caggcatacc aaaaccaagt ttcatcactt caatacactc attcatgaaa cgagcatcta
    41581 aacctgcatg gtaacgtaca gttagattag gttgcggtag atgggtttgt gcaactgatt
    41641 ttaataccaa ataagataat aggttaacag catccttctt atctcgagtc tgtccaccaa
    41701 ttgtaacatt ttgatataaa ggacttcctg ctgaagaaaa tgtatgtgat tgactgcgaa
    41761 ccttattgat tgtaattgtc ttaatccaaa gatttgtcag acgttcaaca atgctatctt
    41821 ctgtttcttt accactttct aaatcagcct tcatatatgg atacatatat tgatcaaaac
    41881 ggccatatga aagagagtgg ccattagatt caatttgtaa aatacattga ataaaccaaa
    41941 ctgattgaat agcttctgca aaagtagttg ccggttcata tgggactcta gagcaaatat
    42001 ctgcaatctc aagtaattct ttcttacgtt taggatttgc attttcggct aaacttttag
    42061 caagagcaac aaagcgcttt gcatatactt taatagcatc gattacaata aatatagagt
    42121 cgtaaaaatg atatttatca atacttgctg gatctgttaa atctagagct actttcgctt
    42181 tacgagcccg ctcttcaaaa cctcttaaac caaattgcaa aagtttctga tagttaactg
    42241 ctaagtgagc atctccagaa ttcatcttac cttccatacc gaagaatcct gtttccatat
    42301 aaacagacac ttcttcaggt aataaggcac cagctctagc acgtaaatta ttattttccc
    42361 aaaacggagc aatacttcta agttgttctt ttgtttcttc tgtaatatag aaaacatctc
    42421 catcacgctt ttcaaaaaga tccaactcat tgagaacaaa ttctagcgta tattccggaa
    42481 aaataggagc atctttattg gaagaagctt gatttcccgc aatcatagat tcttcttcaa
    42541 tatagatagt catattttcc aaaatttctt tcagcatata tgcacgtttt aggacattag
    42601 gttgttcctt gtatcgttca taggcgcgtg ttgctagaac tgctctctca gcatcaatat
    42661 aaggtttttt atttaaaaca tcttctcgat atttgttcat cctttcagta aggctgccaa
    42721 aatattctgt cttgattgtt gttcttgcta cttctgtatt taccatcgta agcacctttc
    42781 tttcgaaaat atttttattt ctttcgaaaa tataataccg atttttatag aaattttcaa
    42841 gcttttaact agaaaaattt taactctcta atttttagca caaatgtgtg agtttaacag
    42901 taacacacaa atacctctaa gattgtttga tttcattttg gtaagctaaa cgatcctgta
    42961 ctttaaagaa tggaaaataa accaatgtag acatcgctaa tatcaccagc tgagtaataa
    43021 ctccttgcca tccacccacc aaaaatcctg ataaaatagc tggtgtactc caaggcaatg
    43081 ttacccctga gaatggctgc atgaaacctg ttgcaatagc tccatatact atcacagctg
    43141 caagtacagg aacaagaatg aaaggtacaa acataactgg attcatgaca atcggaaatc
    43201 caaatacaac tggctcattt acgttaaata ttgctggaaa agctgcaact tttcctaagg
    43261 cttggtattg ttttgatttt gctgcaaaaa gcatggcaac tacaagacca aacgtaatcc
    43321 ctgaacctga tagaattaaa aatgaatcta aaaattgttg agtaacaata tgtgcaccat
    43381 tttctaatga tagattagca gaggctaaca tagctttatt agcatcaaga ttagataaaa
    43441 gcagagctgt cactactcca tttactaccg attgcccatg aacaccaaac caccacaaaa
    43501 atgatataaa gaatgcaatt ccaatagcac catacaaaga tccagttaaa ccttgcaacg
    43561 gaacttgaat agcagaataa atcatttcta tgaatgttcc gccattagtc aatgacttcg
    43621 ctaaaatata tacaatcata gaagataaga aaattacaaa tgctggaatc attgcttcaa
    43681 actgtttggc aatagcttgt ggaacttgtt ctggcatctt aataacaatt tttctcttta
    43741 taaagaaggt ataaatactt cctactacca aacctataat gatagcaccg ataattcctt
    43801 ggcctccaaa ccaaacttta ctaatagcgt ccccaatcgc ctcaccttgt ttagggatat
    43861 aagatgatct tagcaaaata aagaatgcag atacagatag aactccagct ggtaaagcct
    43921 ctactccgct attcttagca taagaatagg caattgaaaa acaagaaatt agacccataa
    43981 tagcaaaagt tcctgaatat acttgcataa acggctctgt ccaattagct ccaaaaacac
    44041 tagcaatgct cttatttaat ccttcgaacg gcaattgtcc cataatcaag aacaaactac
    44101 caactactgt caatggcaaa attgctaaca tcccatcttt tagagctata atgccacgca
    44161 tattcacaaa cttcatcatc ggtgcaatga ttttctgaac atccatcttt gacataataa
    44221 atctcctttt cttacccact aatcaaagat agggccaaat ctaatacttt tttcccatct
    44281 aacataccat agtccatcat cggaataaca gctatcggaa catcacactt atcacaaatt
    44341 tcttttgatt tatctaatgt ataagcaact tgtggaccca atagtgcaac atctatattt
    44401 ggcgcataat ccgctaattt agactgagaa aacgcctcta tttctgcctc aactccacta
    44461 gattgcgctg caattttcat attatttaca agcataccag tagaaaaacc tgctgcacaa
    44521 aacaaaccaa tcttcaccat tatgttttcc tcctctatgt taataacaat gataatactc
    44581 tagtattatt ttttatgaag ttcttttctc aaactaataa tttcttt
//
LOCUS       DAVHEW010000006        43944 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVHEW010000006 DAVHEW010000000
VERSION     DAVHEW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.006"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(389..1615)
                     /locus_tag="VVL53_000323"
     CDS             complement(389..1615)
                     /locus_tag="VVL53_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU4182541.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFKNEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1658..2350)
                     /locus_tag="VVL53_000324"
     CDS             complement(1658..2350)
                     /locus_tag="VVL53_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4182542.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(2523..4274)
                     /locus_tag="VVL53_000325"
     CDS             complement(2523..4274)
                     /locus_tag="VVL53_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182543.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4264..6009)
                     /locus_tag="VVL53_000326"
     CDS             complement(4264..6009)
                     /locus_tag="VVL53_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182544.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6137..6511)
                     /locus_tag="VVL53_000327"
     CDS             complement(6137..6511)
                     /locus_tag="VVL53_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU4182545.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKP
                     GVLYSSKISSNRVNVALPYVGY"
     gene            complement(6530..6667)
                     /locus_tag="VVL53_000328"
     CDS             complement(6530..6667)
                     /locus_tag="VVL53_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182546.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     P"
     gene            complement(6664..7120)
                     /locus_tag="VVL53_000329"
                     /pseudo
     CDS             complement(6664..7120)
                     /locus_tag="VVL53_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7253..8080
                     /gene="xth"
                     /locus_tag="VVL53_000330"
     CDS             7253..8080
                     /gene="xth"
                     /locus_tag="VVL53_000330"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU4182547.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHLEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8092..8235
                     /locus_tag="VVL53_000331"
     CDS             8092..8235
                     /locus_tag="VVL53_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU4182548.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            8346..8474
                     /locus_tag="VVL53_000332"
     CDS             8346..8474
                     /locus_tag="VVL53_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU4182549.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      8857..8952
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9343..9807
                     /locus_tag="VVL53_000333"
     CDS             9343..9807
                     /locus_tag="VVL53_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU4182550.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFATTGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAKGLIQIIE
                     QAGATVQAIGIVIEKSFQDGRDLLEKAGYPVLSLARLDCFENGQVVFKEADL"
     gene            9807..11069
                     /locus_tag="VVL53_000334"
     CDS             9807..11069
                     /locus_tag="VVL53_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU4182551.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11119..11580)
                     /locus_tag="VVL53_000335"
     CDS             complement(11119..11580)
                     /locus_tag="VVL53_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU4182552.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(11577..12341)
                     /locus_tag="VVL53_000336"
     CDS             complement(11577..12341)
                     /locus_tag="VVL53_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEU4182553.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(12522..12926)
                     /locus_tag="VVL53_000337"
     CDS             complement(12522..12926)
                     /locus_tag="VVL53_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU4182554.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(12996..14477)
                     /locus_tag="VVL53_000338"
     CDS             complement(12996..14477)
                     /locus_tag="VVL53_000338"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU4182555.1"
                     /translation="MTLVNKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGAELMDLVTPCPSQVNRDFWATYAHSPE
                     QAIEDFYQLSQKNDYIKLKAIARNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDPAVQ
                     ILAETDRTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(14496..15674)
                     /locus_tag="VVL53_000339"
     CDS             complement(14496..15674)
                     /locus_tag="VVL53_000339"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU4182556.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            15839..16846
                     /gene="galR"
                     /locus_tag="VVL53_000340"
     CDS             15839..16846
                     /gene="galR"
                     /locus_tag="VVL53_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU4182557.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(16892..17929)
                     /locus_tag="VVL53_000341"
     CDS             complement(16892..17929)
                     /locus_tag="VVL53_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU4182558.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(17930..18283)
                     /locus_tag="VVL53_000342"
     CDS             complement(17930..18283)
                     /locus_tag="VVL53_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4182559.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(18527..19417)
                     /locus_tag="VVL53_000343"
     CDS             complement(18527..19417)
                     /locus_tag="VVL53_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU4182560.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGAVVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSDLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            19550..20086
                     /locus_tag="VVL53_000344"
     CDS             19550..20086
                     /locus_tag="VVL53_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4182561.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(20253..21059)
                     /locus_tag="VVL53_000345"
     CDS             complement(20253..21059)
                     /locus_tag="VVL53_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU4182562.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(21404..22924)
                     /locus_tag="VVL53_000346"
     CDS             complement(21404..22924)
                     /locus_tag="VVL53_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU4182563.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVSKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(22917..23645)
                     /locus_tag="VVL53_000347"
     CDS             complement(22917..23645)
                     /locus_tag="VVL53_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182564.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(23660..24424)
                     /locus_tag="VVL53_000348"
     CDS             complement(23660..24424)
                     /locus_tag="VVL53_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4182565.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(24428..24871)
                     /locus_tag="VVL53_000349"
     CDS             complement(24428..24871)
                     /locus_tag="VVL53_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4182566.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(25553..25849)
                     /locus_tag="VVL53_000350"
     CDS             complement(25553..25849)
                     /locus_tag="VVL53_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU4182567.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(25871..26935)
                     /gene="pepA"
                     /locus_tag="VVL53_000351"
     CDS             complement(25871..26935)
                     /gene="pepA"
                     /locus_tag="VVL53_000351"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU4182568.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            27336..28037
                     /gene="galE"
                     /locus_tag="VVL53_000352"
     CDS             27336..28037
                     /gene="galE"
                     /locus_tag="VVL53_000352"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU4182569.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            28460..29419
                     /locus_tag="VVL53_000353"
     CDS             28460..29419
                     /locus_tag="VVL53_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4182570.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTHKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            29409..30365
                     /locus_tag="VVL53_000354"
     CDS             29409..30365
                     /locus_tag="VVL53_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU4182571.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFVLLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFILANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            30362..31114
                     /locus_tag="VVL53_000355"
     CDS             30362..31114
                     /locus_tag="VVL53_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4182572.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            31210..32175
                     /locus_tag="VVL53_000356"
     CDS             31210..32175
                     /locus_tag="VVL53_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4182573.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELTKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(32400..32642)
                     /gene="yidD"
                     /locus_tag="VVL53_000357"
     CDS             complement(32400..32642)
                     /gene="yidD"
                     /locus_tag="VVL53_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU4182574.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(32642..33364)
                     /locus_tag="VVL53_000358"
     CDS             complement(32642..33364)
                     /locus_tag="VVL53_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU4182575.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(33351..33920)
                     /locus_tag="VVL53_000359"
     CDS             complement(33351..33920)
                     /locus_tag="VVL53_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU4182576.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(33939..34667)
                     /locus_tag="VVL53_000360"
     CDS             complement(33939..34667)
                     /locus_tag="VVL53_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU4182577.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLTFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(34667..35407)
                     /gene="xerD"
                     /locus_tag="VVL53_000361"
     CDS             complement(34667..35407)
                     /gene="xerD"
                     /locus_tag="VVL53_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU4182578.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(35398..35859)
                     /locus_tag="VVL53_000362"
     CDS             complement(35398..35859)
                     /locus_tag="VVL53_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4182579.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(35856..36377)
                     /locus_tag="VVL53_000363"
     CDS             complement(35856..36377)
                     /locus_tag="VVL53_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4182580.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(36353..37324)
                     /locus_tag="VVL53_000364"
     CDS             complement(36353..37324)
                     /locus_tag="VVL53_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU4182581.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(37321..38115)
                     /gene="racE"
                     /locus_tag="VVL53_000365"
     CDS             complement(37321..38115)
                     /gene="racE"
                     /locus_tag="VVL53_000365"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU4182582.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(38322..38570)
                     /locus_tag="VVL53_000366"
     CDS             complement(38322..38570)
                     /locus_tag="VVL53_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU4182583.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(38826..40451)
                     /gene="treC"
                     /locus_tag="VVL53_000367"
     CDS             complement(38826..40451)
                     /gene="treC"
                     /locus_tag="VVL53_000367"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU4182584.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(40629..42596)
                     /gene="treP"
                     /locus_tag="VVL53_000368"
     CDS             complement(40629..42596)
                     /gene="treP"
                     /locus_tag="VVL53_000368"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU4182585.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            42781..43491
                     /gene="treR"
                     /locus_tag="VVL53_000369"
     CDS             42781..43491
                     /gene="treR"
                     /locus_tag="VVL53_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU4182586.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            43646..>43944
                     /locus_tag="VVL53_000370"
     CDS             43646..>43944
                     /locus_tag="VVL53_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4182587.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKN"
BASE COUNT        12820 a        10130 c         8432 g        12562 t
ORIGIN      
        1 aattctatca tgcaaaagaa tgactagaac atgaaagaca ggcacaaaac tttgaacaat
       61 attctgaaaa caaaaaaaga cgcgagtttc cacaatctca ctcatggaaa ctcacgtctt
      121 tcaaaaaata gggtgaattg aaaactagaa caagacacta gattttctaa atgatgtcta
      181 gtaaggattc taaatgctac accaaattgc tcacatctta tttcaaacca cgggactccc
      241 acaagaaagg aaaatcctat aaaaattctc tctctgagga gaaagaggcc tcaactacag
      301 gtgtaaacta ttatcaattg aaaacaacag tttttacact caattagact ttttagggac
      361 aggatcactt tcgcttgact gtagtttgtc atttttttga taaagttagc actttttcag
      421 aaactgtttt gaaagtctca atgatatagt ctacttcttc atcgcttaat ttagtatgaa
      481 gagggagggt aatttcattc ttaaagaagg cataggcctt aggatagttc gtcatatcaa
      541 atccaagatt cttataggct gtcaagagag gaagcggttt gtagtgaaca ttacttgcaa
      601 ttcctgcttt agccaattct tggatgatga ggttgcgttc ttctaggctt gctccttcta
      661 cacgggtgat gtagaggtgg cgtgaagatt cgacagtttc agtcttgtgt gccaaaggat
      721 ggatgcgaga acctgcaaaa ccactatcat agcggtccac aatgtcctta cggcgttgca
      781 acaaacttgg atagcggtcc aattgtacca aaccaagtga agccatgata tcggtcatgt
      841 tgcacttata ggctggtgta acgatatcgt attcccatga ccccagttgc atcttggcaa
      901 gagcatcctt agtttgcccg tgaagggaaa ggatttggaa ttccttgtac atctcttcgt
      961 catcaatcac tggattggct ttccaagtcg cacttccacc ttctgccgtt gtaaagttct
     1021 taacggcatg gaatgagaag gaagtaaagt cagcgataga accagaaggt tgtcctttat
     1081 aagtagatcc caaagcgtgg gcactatcag agacaatgac aatacggtta aaggcctttt
     1141 gccacttgct tgaagcggta aagaagtcac gttttttctc cacgacttgg aacaaacggt
     1201 cataatcgca aacaatccct gcgagctcta ctgggataat caccttagtt ttctcagtga
     1261 tagcttgctc aagcaggtca tagtccatct caaacgtatc tgcttggata tccaccatga
     1321 caggggttgc tcccacgtgc gtaatgacac tacatgaagc cgtataggtc atggctggaa
     1381 cgatgacttc atcaccaggt cccacttcca aaacgcgtaa aatcaactcc agagcagctg
     1441 tcgcagagtt gagacaaaca gtcttaggtg tctgggtgta aagagacaag cggcgctcca
     1501 gttcttttgt tttaggacct gttgtgatcc aaccagaacg cagggtatcc gctacttcag
     1561 caatttctgc ttctgtgata tcaggcggtg aaaatggaat attgtaattt ggcattgatt
     1621 tgctcctttt atctgtactc attttcttat gactacttta ttttagtacc tcaaacacgg
     1681 tttgaaacat gattttgatg tccccaaaga aactaaactc tcggagatag gcgaggttat
     1741 agcgcatctt ttcaggaaga acatgctcca cataggcctg atcaactgac agacctttct
     1801 ccgtcatttg actgatgatg gtgtcctcat ccttgtagtt gatgctggct ggagaggtaa
     1861 ttcctgcttg caagagcaag gttgccatca tttcagggct atactgctct gtataacgtg
     1921 gcacttcagg tcgtgtaccg acaaaggaca tctcaccttt aaggacattg accaactgag
     1981 gcagttcgtc caaacggaca cgtcggatga aatttccaac cttggtaata cggctatcgt
     2041 tagcagaagt caccagactt ccttttttat ccgcatccgt caccatggta cggaacttcc
     2101 aaatcttgaa ccgacggttg tactgggtca cacgctcttg cttgtaaatc actggcccct
     2161 tgctatccaa cttgatccaa atgctcaaga tgagaaagat gggagaggtt agaaccagta
     2221 agaccaaggc caaaaaccag tccaagcaac gcttgaaaat cagcgaaccc ttccttttag
     2281 agacaagctg gtagtaagac tcaacctcgc ttgatttcat ttccacaggc aagtcttccc
     2341 atttcagcat actgttctcc tttgttttta ttatactatt tgtcaacatt tttcattata
     2401 ccacaaaatg gaaaaggcgg tgaaagaaat ctgtaatttg aaggatttcc ttctagtact
     2461 caatgaaaat caaagtacag ctttgagatt gcagataaag ctgacatggt ttaaagagat
     2521 tttcaaagag tataggccat ggcccctgcc tgcaaactat acatcttgtg ataggttcct
     2581 cccagagtca agagttcctc atgggttcca ctctcgataa tgcgtccctt atccaagaca
     2641 tagatgcagt tggcgtcttg aatagtagaa aggcggtgag cgatagcaat agttgttcgg
     2701 ccctgtctca tcttcgccag agaagcttga accaagcttt ctgtttcaga gtcaatattg
     2761 gctgtcgctt catccaaaat caggatttta ggctggctgg cgactgttct agcaaaggca
     2821 agaagctggc gctgcccagt agagaagctc gaaccacgct cggaaacagg ggagtcgtac
     2881 ccctgaggaa gttcttgaat aaaggaatct gcatccacaa aggctgccgc agcctgaacc
     2941 tgctcatcac tgatttcttg gtacatggcg atattggact taatagttcc atgatagagg
     3001 aagggttcct gtaaaaccaa accgatgttt tttctcagct cttcttgact gaaatccctg
     3061 atatccacat catccaagag aactctccct gactggaatt cataaaagcg catgaggaca
     3121 ttgataatag acgatttccc tgaacctgta tgacctacaa aggcaatggt ttcaccctta
     3181 ttaacagaga aagaaatgtc atccagaatc ggatgtttac cgtcatacga gaaacacaca
     3241 tgttcaaaac ggatattgcc ttctttgact ttggcttgcc cattttcttg aagaggctca
     3301 taggttctct catcgattaa ggcaaagaca cggcctgcag aaaccataga cgtttgcaga
     3361 gttgaaaagt tttgcgtcac ctcaatcaag gggtcaaaaa ggcggttgat gtactggata
     3421 aaggcataca tggtcccggc cgttatcccg atagaaaaac cacggtagcc aaagtaggcc
     3481 atcaagactg catagcctag aagtttcagc aaactcatgg caggtctcaa aaagagggca
     3541 tccaaggcta cagaacggtt ggcgtagacc aagtgttctt ggttgatttc atcaaattct
     3601 gcctgcaggc gcttctcttg attaaaggcc tgaataatcc tgattccctc gatattctct
     3661 gccagcttac tattgatatc tgacaagaga cttctggttt tctcgatgat tttcactgac
     3721 ttttttcgat agagattgac caaaaggaaa atcaaaggaa gaaagagcaa gactaaagcc
     3781 gtcaaacgaa aatccagcac caacatggta taaagggttg tcagaaagat aaaaactgct
     3841 gagataaagc tggataaaat cccagaaaac atatcactaa tcgtctcggt atcgttggtc
     3901 aaacgagaaa cgatagaacc tgctggcgtc ttgtcaaagt aagacatgcc cagtttctcc
     3961 atattggcaa aggcatcccg acgaatatcc ctaacaatac tgtaagacac gcgcgcaaag
     4021 agaagattgc ccacatactg aactacagtt tgtaggatgt agagaccata gtagaccagc
     4081 aaaacggtaa cggctagttg gttaagattg ctgagatact ggtcgataaa gtgggaagcc
     4141 acgaggggta tgacactttt aatgaccgtc gtcgctagga gaaaactgag tgccaaaaag
     4201 gtcaggagtc cataaggctt gagataagac atcaagcgct tcaatacagc ccattgttct
     4261 tgtttattct gcatcttctt ctcctttcat ttccaactgc tgagactggt aggtttgggc
     4321 ataccagcca tccatcgcta gcaagtcttc gtgcgtgcct cgttcgataa tttgaccatt
     4381 ttgtagaact aaaataaaat ctgcatggac aacagcactg aggcgatggg cagtgataat
     4441 ggttgtcttg tcctttcgcg tctccttgag gttgtcgata atcgcatact ctgtcttggc
     4501 atctacggcg gataaggaat catccaaaat caagatatca gggtctaaaa tcatagcccg
     4561 actcatagcc aaccgttgct tttgaccacc agaaagactg actccttttt caccaatcag
     4621 cgtatcaaat ccttgaggca tgtctacaat atcttggtaa acccgggcta gcttagtagc
     4681 ttcctcgacc gctgaaaggg gcaagttagg attgccaaag cggatattgt ctaggattga
     4741 agtcgcaaaa agaaactggt cctgaggaac atagcccatg agactgcgaa ggtctgtcag
     4801 acgatagtcc cgaatatcgt gaccgtttag ataaatggca cccttatcca catcgtattc
     4861 acgcaagagg agcttgatta aggacgtttt cccagagcct gtctgcccaa ccaagcccag
     4921 tgtttgccct tttgccaaac taaagtgaat atccgtcagt gtttcctcat tttcaaaagc
     4981 aaagctgtca atggcatact ccaaacgccc attttcaata ccatccagag gaaactccgg
     5041 gtcttgtaca ggagattcct gagacaaaag attttcaatc cgctggtaag aaaccttccc
     5101 tcgctgagta gtattaaaga ggaagccgat ggccataaga ggccagacca gcatatccaa
     5161 atagctgata aaggtgacta gattcccaac tgtaatctgc ccttcctgaa ccaccaagga
     5221 gccaaccaaa agcgttaaaa cataggacga accaacaaac aagagaacca tagggtcaaa
     5281 gagactatca tatttcatgg tttgcaggtt cttttggaag gttaattcat tgactgcctg
     5341 aaaagacttc aactcgtctg cctgataacc gaaagacttg gtcactttga tacctgatac
     5401 ggactcctgt accttgttat tgagttcaga aaaagcagct tgggattcgc caaaggcctt
     5461 atgagtcttt ctccctaggc gactagtcgc ataggccatg aaaggtaggg ggagaatggc
     5521 aacaagagtc atctgccatg agatgctaaa gagcatggtc aacaaagtca ccagagccgt
     5581 gatagaggca tccaccgcag acatgacacc gccacctgct aaacgagtca aggcatttat
     5641 atcattggtt gcgtgtgcca tcagatcacc cgtccgatag gtttgataaa aggctgacga
     5701 catttttgtg aaatgcttaa acaagcgaga ccgcatgatc tgtcccaagc aataagaggt
     5761 cccaaggata tacatacgcc acacatagcg caaatagtac ataccaaagg ctgcaagtag
     5821 caagtaaaat aggctaagaa ggaggtcctg ctgggttaat tgccccgatg tgatggcatc
     5881 aataacccgc cccataacca taggaggaat gagattgagg acggaaacca agaccagggc
     5941 cacaattccg actagataac ggcgtttttc taacttgaaa aaccaccaaa gtttttgaat
     6001 aatggacata aaatcccttt ctgattgcaa atggaaacct gaggccaata ctcaatgaaa
     6061 atcaaagagc aaactaggaa actagccgca ggctgctcaa agcactgctt tgaggttgta
     6121 gatagaactg acgaagtcag taacctacat acggcaaggc gacgttgacg cggtttgaag
     6181 agattttcga agagtacaag accccaggtt tttctttttc ataagttacg actgtaacag
     6241 cacccttgtc atattcagca ataaagatat tggctacatt gtcatgccct tgtttactga
     6301 ggttatcaag caaccactct tcgctacgac caatcgattc caagacatct acttgaatca
     6361 caccgtcagt cacaactgga tacttaggat tttcatctcc catttgcaca acgatgagtt
     6421 gcccattttg ctcttgcaca gctcgtttga cttgcttcat ctggaaaatc ccttggctac
     6481 gaagtttgag ggctacatcc gatgcagaca aaccaactga acgacaggtt tatgggtcaa
     6541 tattcccatt tttgataagg agagttggct taccatcaat caaacgtttc acaaagtgaa
     6601 cattgttgtt gagccatttg agagtcaaga ccagtttgat taaaatttcg atataattaa
     6661 gtgtcatttg ttcacatcca ccagttcaat tgcgtctgtc ttatacaatt cgactttctc
     6721 taacaggtac ttgtctggct cacttccgtt tagggcacga taatagcgat ctcccacctt
     6781 gataagtgcg ccatctgtgt ttgtggaagt attaacatag acttctgact tgtctactcc
     6841 caaatctttg gaaacaacct cgatgaaatg aagtgaagtt tgaaatttat tgttagaaac
     6901 ttgattgttt tgataagtcg tgatgctgac caaaagcaga gctaaataag gccagagctg
     6961 aaatcatgac caactcacga aattcggttc cctttttatc atggtatgcc ttaaaagcaa
     7021 aaaaaactgt cacagctaat agaacaatcc caaagccaac catgatacca ttttgctgac
     7081 cgatttggct gagtacatag tcataagagt agaatttcat gtattttcac tccctttcat
     7141 aaaatcattc ttaattataa acgaaagaga gtaatttttc aaactatacg ttggggaaat
     7201 agatcttttt ttggtataat aaaatctata atctgaatga aaaggtaact ttatgaaact
     7261 tatctcatgg aatattgatt ccctaaacgc tgccctaact agtgactcag ctcgtgccaa
     7321 attgtcccaa gaagtcctac aaaccttggt cgctgaaaat gctgatatca ttgctatcca
     7381 agaaaccaag ctttctgcca aagggcctac aaagaaacac ttagaaattt tagaagaact
     7441 cttcccaggc tacgaaaaca cgtggcgctc ttcccaagag cctgcccgta aaggctatgc
     7501 tggaaccatg ttcctttata agaaagaact tacacctact atcagcttcc cagaaatcgg
     7561 tgccccttct accatggact tggaaggtcg tatcatcact ctagaatttg atgcattttt
     7621 cgtaacccaa gtttacactc caaacgctgg tgacggtctc aaacgcttgg aagaacgcca
     7681 agtctgggat gccaaatatg ctgagtattt ggctgaacta gacaaagaaa aaccagtcct
     7741 tgcgaccggt gactacaacg tagcccacaa tgaaatcgac cttgcaaatc ctgctagcaa
     7801 ccgccgttca cctggattta ctgacgagga acgtgctgga tttaccaacc ttttggcaac
     7861 tggatttaca gacaccttcc gccatgttca tggcgatgtt cctgaacgct acacttggtg
     7921 ggcacaacgc agcaaaactt ctaaaatcaa caatacaggc tggagaatcg actactggct
     7981 cacaagtaac cgtatcgctg acaaggtcac taaatctgat atgattgact caggtgctcg
     8041 ccaagaccat acaccgattg tcttggaaat tgacttgtaa ggattttcta tatggactac
     8101 aatgcggtca ttcccgagtt gcttgtatcg aatatcgaac agtcccggtc cttctactgt
     8161 ggtttactgg gttttaggat tgaataccaa cgtccagaag aaaactttct ctttctcttg
     8221 aagagtgtca actaatgtta gaggagggaa caaaggatca actagctgaa ctgacctatc
     8281 cctttggtcg tggagtcaat ctctcctttg gcataaagga tgtttcaaaa ctctattaaa
     8341 gagtgatgaa ggcaaactat cctatttatc gtcccttgac caaaagaaag tttcgtgtca
     8401 gtgatcccta tatctatcct cataaatttg cagttttgga tccagatggc tattttttaa
     8461 gatttagcga gtagaataat aaaaattggg aaaagtcttt aaagttagaa aaaggcatat
     8521 tgtcaggtgt ctaaaagctt gatagtatgc cttttatgat ggaaaaatct tctaatactc
     8581 ttcgaaaatc tcttcaagcc acgtcaacgt cgccttaccg taggtatatg atactggctc
     8641 tgtcaattct atccacaacc tcaaaacagt gttttgaggt tgtggatagc ttcctagttt
     8701 gctctttgat tttcatttag tataactgtt agcttacaat ggagtttttg ttcaccctct
     8761 cttctttttg ttcgtttttt aaaccaaaac actaacaaaa tagttgattt ctaaagttat
     8821 ttagtgtaaa ataaaaacat tggctcaagc ctatttaaaa ttgaatatcg ttttacttgt
     8881 ttatgtcgtg aattggcacg acgtttctac aaggtgccgg aacacctaac aataagtaag
     8941 tcagcagtga gatatggccg tttttgccat gtctatttag gggacttact tagagattaa
     9001 gtaggtcttt ttttaatatt tcccttaatt agtgcggacg ggaggtgaaa ttatcctgtg
     9061 aataatttca ggaatccagg gaatttcatc cctaattttt gagcccctag ctctttcatt
     9121 tctaggctta ggctaaaata gttccccgaa ctattttact ctcaaaaatc ccgtcataga
     9181 caataacaag attattgtct atgataccac tacaacggga aatatttgat aagactcgct
     9241 tcgctcgtaa tagttttatc aaatatatag tattagcaaa agggatcact atagaaatat
     9301 ttaaacaaca agtaaattaa cttttagact ttaggaggtc ttatgaaatt attagaagag
     9361 cgcatcctca aggatgggca tatcttgggt gataacatcc tcaaggtaga ttccttttta
     9421 acccaccaag ttgactttag cttgatgcga gagattggta aggtttttgc ggaaaaattt
     9481 gctactactg gcattaccaa ggtcgtaacc attgaagcgt cgggtattgc cccagccgtt
     9541 tttacagctg aagccttaaa cgttcccatg attttcgcca aaaaagctaa gaacatcacc
     9601 atgaacgaag gcatcttaac tgctaaaggc ttgattcaaa tcatcgaaca ggccggtgcc
     9661 acagtccaag ctatcggtat cgtgattgag aaatccttcc aagatggtcg tgatttgctt
     9721 gaaaaagcag gctaccctgt cctatcactt gctcgtttgg attgttttga aaatggtcaa
     9781 gtcgtattta aggaggcaga tctctaatgc aaactcaaga aaaacactcg caagcagccg
     9841 ttcttggctt gcagcactta ctagccatgt actcaggatc tatcctggtt cccatcatga
     9901 ttgcgacagc ccttggctat tcagctgagc agttgaccta cctgatttct acagatatct
     9961 tcatgtgtgg ggtggcaacc ttcctccaac tccaactcaa caaatacttt gggattggac
    10021 tcccagtcgt tcttggagtt gcattccagt cggtcgctcc cttgattatg attgggcaaa
    10081 gccatggtag tggcgctatg tttggtgccc ttatcgcatc tgggatttac gtggttcttg
    10141 tttcaggcat cttctcaaaa gtagccaatc tcttcccatc tatcgtaaca ggatctgtta
    10201 ttaccacgat tggtttaacc ttgatccctg tcgctattgg aaatatggga aataacgttc
    10261 cagagccaac tggtcaaagt ctcttgcttg cagctattac tgttctgatt atcctcttga
    10321 tcaacatctt taccaaagga tttatcaagt ctatctctat tttgattggt ctggttgttg
    10381 gaactgccat tgctgctact atgggcttgg tggacttctc tcctgttgcg gtagctccac
    10441 ttgtccatgt cccaactcca ctctactttg ggatgccaac ctttgaaatc tcatctattg
    10501 tcatgatgtg tatcatcgca acggtgtcta tggttgagtc aactggtgtt tatctggcct
    10561 tgtctgatat cacaaaggat ccaatcgaca gcacgcgcct tcgcaacgga taccgcgcag
    10621 aaggtttggc cgtacttctc ggaggaatct ttaacacctt cccttacacc ggattttcac
    10681 aaaacgttgg tttggttaaa ttgtcaggca tcaaaaaacg cctgccaatc tactacgcag
    10741 ctggtttcct ggttctcctt ggactgcttc ctaagtttgg cgcccttgcc caaatcattc
    10801 caagctccgt cctcggtggt gccatgctgg taatgtttgg ttttgtatca attcaaggga
    10861 tgcaaatcct cgcccgtgtt gactttgcta acaatgaaca caacttcctt atcgcagctg
    10921 tttcaatcgc tgcaggtgtc ggtctcaaca acagtaatct ctttgtcagc atgccgacag
    10981 ccttccaaat gttcttctca aacggaatcg tcgtagccag cctactcgct attgtcctca
    11041 atgccgtatt aaatcataaa aagaaataag aaaaagaggt gcgagcctct tttttgttga
    11101 caaaataaaa acatctacct attcatatat gctagtgtca tagtcctgaa aatcatctgc
    11161 atcaagaaca atttcacaac tcttatactt ttctcttaca agtcgttcgg cttcatctgg
    11221 attttcagcc tctatactta ctaaacgtga taaagtttct gtaatttcta ctgtatactt
    11281 tttcagtttt agattgcttt ccaataattc acctattaac aataatctat gataggagct
    11341 atgtagccac tctggcaatt tatctcctac ttcccaccca tatttttcct tcatcaaagc
    11401 tagttgtttt tcgtcaccaa atttcatatt ttcagataac catctcacac aatcgggatc
    11461 tgataaggta cagacgatta aacttaggtt atcgtaacgg ctttcagttt cataatcagg
    11521 ccagatgact tttaagaaat catacacctc ttctgatgca acaagttgtt tcgttttcat
    11581 aatttccgat actttcctct acctttaaat tctattattt ctttgtctct taatatttga
    11641 agctgttgcc taatcttttc tttgatatga ttgttcttaa caaagatatt ttttaggtca
    11701 ctttcaaaac gatacatatc ttcaagggta aattctgaac cctctatctt atctatacaa
    11761 tttagaattt ctattgtcca acctcttgat gacagagagc tcttccttaa aaataaacct
    11821 tgcttaaatt cttttgtaac tttttctgga tctctaactt gtccatcttg cacaagaaat
    11881 atccttcctt tagaaggtac ttgtgataaa tcaatgttac aaccaatcca acctgctcgt
    11941 ctagcagttg gtgcaagtgg ttttctttga ataatcgatt tcggtgtaac aaattgcttc
    12001 ggaaggacaa gaaagttatt tacctcaaaa ttttttgtgt aagttaaaaa aaagaaatta
    12061 ggattattat ctgcctgcac acgcttcatc atcgttgcat aagcaccatc attgattgtt
    12121 gatgaaaaat ttcctttttt gctctttagt tcaaactcct cactacaatg attacagtaa
    12181 aaatctgcta caggccgatt attttcaaaa tgatttaagg gattgttccc acaattaggg
    12241 caataagact gacgataaac ccaatcctcc gttaagatac gtgccttttg gctattactt
    12301 ttataggttt ctactaattc taaattaaaa tgtaattcca tttaaaaatt cctcactaat
    12361 atgagaactc actcgatata agcaattaaa atctactata actagtatac cacatatatg
    12421 tacctattac atttgtattt catcactctc ttttatattt ccttctaaca tatctcataa
    12481 attaaaaatg agtgagataa atttatccca ctcacctttc tttatatcct tacccgtctt
    12541 tcctcagacc tttggcctgt gacaaacatg gtaaaggttg ctttgcagac atttctgccc
    12601 tcttgattgg tgatatccac atccattaca caagttgtac gaccttgatg gacacattct
    12661 cctttaatgg tcaatacatc gtcgagtttt cctgccttaa ggtagttgat agaggattgg
    12721 agtgtcaccc catcaagtcc cagcgagata accaccaaac cactgatttg gtcacatagc
    12781 gtgaatagat agccaccatg ggcattgcca tagtagttga gcgacgagtt cactactttg
    12841 gtcgtcacca caacgtgacc atctctcatt ttttcaattt cgtaattttc aaaggcagat
    12901 atagcgtcaa aatgaaagtc tttcatcgtt tcctcctgat atttcaaacg acactatgat
    12961 actacttttt ataggactgt gcaaggagaa agctcctagt ctagtaaaat tccgacctgt
    13021 tccacaaagc gcataaaggc tgtctgccct tgttctgtct gcttgtagac tcctgcatcc
    13081 tcaagtacac gcgcaaagat agcacccaca gagtccttga cgattgcaag ggctttttct
    13141 ttatccgtta ggtctggatg ttgggatttg agttggtctg cccactcctg atgataatcg
    13201 gcaactgtaa cagcttctcc tacaagatag ctagcgactt gctccacttc ttctttcaga
    13261 cgtggtggca agattgccaa gcccatgacc tcaatcaagc cgatattttc cttcttgata
    13321 tgttggacat ccttgtgggg atgatagata ccatcaggat actctgctga agtctgattg
    13381 tctcgcaaga ccaagtccaa ctcaaactgt ccatcgcgtt tgcgggcaat gggtgtgata
    13441 gtgtgatgcg gtgtcctgtc tgtctctgcc aaaatctgca ctgcaggatc tgaatactgg
    13501 cgccattcct gcaaaatctt atcagccaaa ttgatcaaat cctctttgga atccgaagtc
    13561 aaacgtagga cagacatggg ccacttgaca attccagcct tgacctgctc aaaaccagca
    13621 aatcggaagg ccttttgcaa gggagccaat tccataggaa atacgtgacg gcctccctga
    13681 taatgatcat gagttagaat agagcccccc acaatcggca ggtcggcatt agatccagca
    13741 aaatatcctg gaaactggtc tacgatagcc aacagacgtt caaaactctg acgactaatg
    13801 gccatgggac gatgctggcc atctaaaaag atacaatgct cattaaagta agcatagggc
    13861 gaatactgga aaccccattc ctgaccaacc atttcaaaac ggataatacg gtgattgcta
    13921 cgagctgggt ggttaactcg accatggtag ccctcattct ctagacaaag ctgacactga
    13981 ggataattac tagcttgcac caacttggct gccacaatct ctttgggatc tttttcaggc
    14041 ttagagagat tgatggtaat ttcaagttct ccgtagtcag atggaacacg ataagcgata
    14101 tttctagcaa tggccttgag tttgatgtag tcatttttct gactgagttg gtaaaaatcc
    14161 tctatcgctt gttctggaga gtgggcgtag gttgcccaaa aatcacgatt gacctgactt
    14221 ggacaaggag tcaccaaatc catcagttca gcaccaagga tttcacgcgc agtctgacta
    14281 tcctcaatcg tctctaatcg aacggcttct tcaaccagct ggtccttgag gtcaatcaat
    14341 ttatccagat tggtctcaac ttccaaaaca ccttctccca ctcgtgccaa aacacgattg
    14401 gtcagataga ttcgatccat ttcctcaaat gagctttcag aaatgacatg tgttacaaat
    14461 ttatttacta aggtcactat agagcctcct tttgactagt caaggacgcg agtgccacct
    14521 gcaacttcag cgatatagaa gcttggagcg tatccaacta cttcctcgta gtgtttgcct
    14581 acagcttcct taaaggcctc aacagtatct ttttgaacca aggcaatggc acagccacca
    14641 aaaccagccc ctgtcatacg agcaccgaga actccttctt gtgcccaagc tgtgtgaaca
    14701 agggtatcca attccaaacc agttacttca taatcatgct ccagagaaac gtgtgacgca
    14761 ttcatcaagc gtccaaatgt ttccaaatct cctgcttgga gtgctacttg agctttgagg
    14821 gtacgttggt tttcaagcac agcatggcga gcacgtttca aacgattttc atctttaatc
    14881 agatagctat attggtcaac ggcccactcg tccaattcac ccagagtctg aatatccaag
    14941 gaaacttgca attcttccac tgctttttca cactcagcac gacgttcatt gtatttagag
    15001 tccgccaatt cacggcgttt gttggtgttc atgataacaa cgacattgtc cttcaaatca
    15061 agtggcacca agtcgtattc taaagtatta gtatctaggt aaatagcacg ttggtctgcc
    15121 cccataccaa tagcaaactg gtccataatg ccagagttta ctccgataaa gttgttttct
    15181 gtttgtttgc cgattttaac caaatcgaga cgctctaatt ttaaatcaaa gagatgctca
    15241 gccacgactc ctgtcaagag ttccaaggat gcagaagaag acaagccagc accatttgga
    15301 atatttccat aaacataaaa atcaaaacct ttgtcaatca cgtgcccagc ttcttgcaag
    15361 aaatgaagga caccttttgg ataattggtc cagttgtgct ctttttcaaa cttgaggtca
    15421 gcgagaggca cttcgataat gcccttgtcc tcaaagttag ctgagtagaa acgcaagact
    15481 tggtcgtcac gcttacgagc tgcaccgtaa gttcccaagg aaatagcagc aggaaaaacg
    15541 tgcccaccgt tgtagtctgt gtgttcacca atcaaattaa tgcggcctgg tgaaaagaag
    15601 gtttgatctg cttcttgacc aaaaacagca agaaagtctt tgcgaagggc ttcagtagta
    15661 agatgttgtg ccatatgaat tctcctttga ctgtccgtta agccatctta acgtgtaatc
    15721 gttttctttc tattatatcc aaggaatttt ccaaggtcaa tcctttttac taaaatttta
    15781 gtaaaatatc agtaaaaaga agaaaaacat gatatactaa cctaatcaag gaggatttat
    15841 ggctacctta aaagacattg cacagctagc ctctgtctct atcgcgaccg tatcccgcgt
    15901 cctcaaccgc gaccagagcc tatctgttac agaagaaacc agacaccgta ttttaaccgt
    15961 tgctgaagag ctgggctaca ccaagcacct caagacaggc gagtcccaca aacccaagca
    16021 aaagattgcc attatccaat gggtcagcga acaaggggag ctggacgacc tctactacta
    16081 ccagattcgc ctaggaatag aaaaaagagc ccaagagttg gactatgata tcttgcgcta
    16141 ttttaatgac caccctttta ccctaagcga ggaagtgatt gggattctct gcatcggaaa
    16201 gtttagtcga gctcagattt ctgcctttga agaataccaa aagcctcttg tatttctaga
    16261 cagcgataca ctttccctgg gacatacctg tattatcacg gatttttaca ctgctatgaa
    16321 acaggttgtc gattatttcc tcagtcaagg aatggaccgt atcgggattc taacaggcct
    16381 tgaagaaaca acagaccaag aagaaatcat tcaggacaag cgtctagaaa acttcaaaaa
    16441 ctatagtcaa gcgaggggaa tctatcatga tgaactggtc tttcaaggaa gatttactgc
    16501 ccagtctggc tatgacttaa tgaaggaggc cattcagagc ttgggagacc aacttccgcc
    16561 agcatttttc gcagccagcg atagtttagc tatcggtgcc ctccgtgccc tccaagaagc
    16621 tggaatcagc ctgccagatc gcgtcagcct catttccttt aacgacacta gtctgaccaa
    16681 acaggtctat cctcccctct ctagtattac agtttatact gaagaaatgg gccgagcagg
    16741 tatggatatt cttaacaagg aagtcctcca cggtcggaaa atccctagcc tgaccatgct
    16801 gggaaccaga ctgacattaa gagaaagtac cctaaatcaa gaataggata acataaaaaa
    16861 cgaatagagt tctaaaactc ctattcgttt tttattcgat tacaatcata gacttaattg
    16921 tcttacgttc atccatatct ttataggctt ggtcgatatc ttccagttta taacttgaag
    16981 taaagacgcg acctggattg atatcaccat caaggacggc ttttagtaaa aattgcttat
    17041 cgtatgttgt agcagaagct gccccacctg ctacagagat attttgcata aatgtcgaac
    17101 caagagcacg attattatag tgtgggactc ctacaaagcc catacgccct ccattatgaa
    17161 gaacacctag cgcctgttct atagcagcct ccgtaccaac acattcaagt gctgcatctg
    17221 ctcctccacc gaggatttca cgcaccttgg taattccttc ttgaccacgt tctgcaacaa
    17281 cagctgtcgc acctgactcc atagccatct tttgacggtc ttcatgacgg ctcataagga
    17341 taatttgtga tgctccacgc atcttagccg cgatgacagc acattgacca acagccccat
    17401 caccgataac aacaaccttg tccccttttt gaacatttgc aacacgcgcc gcatgatagc
    17461 ctgtcggcat gacatctgca agagtcaaaa gggacttgag catcccttct gtatagtcag
    17521 aaggttgacc agggatttta accagcgccc agtttgcata gtggaagcga atatattctg
    17581 cctgaaaatc accccccaaa ttattgccaa tatgattgtc gcaagaaccg tcaaatccag
    17641 caagacaggc atcacactca ccacatccat gtgtaaaagg gacaatcaca aaatcacctg
    17701 gtttcaccgt cgtaatggct tccccagctt cttcaacaat cccaatcgct tcgtgtccac
    17761 tatttttgtg tccagctttc gtttctggat tacggtacct ccataaatct gaaccacaaa
    17821 cgcacgcacg aaccacacga ataatcacat catccgcttc tattatttgc ggacgttcaa
    17881 tgctagcaag tccaacctga cctgcctttg tatatactgc tgatttcatt taaaattttc
    17941 cttccttata aagtttaatt ttgagattta aacgatttaa agctgtctgt agctgagaca
    18001 agcgctcctc taatttttgc ttttcctctt ctaaaatacc aagcctctcc tctctcgttt
    18061 catctccctt ttggtagagc gacatatagt caactaaact atctacagag acacccgccg
    18121 aacgaaaaca cttaataaat tccagcgctt cgatatcctg atcttgaaaa tcacgaatcc
    18181 cagtagcagt acgagtaatc ggtggcacaa gaccaacccg ttcataatac cgaatcgtat
    18241 ccgctgaaat tcccaacaaa tcactggcag atttaatatt cacacgctaa cctcctctca
    18301 tttcattcac tgaattcacg ctattctaac tcacttatct tattatataa ctttgagttg
    18361 actccaagtc aagcattttg tatctaatcg aaaaattttt atcattaaca atcatatatc
    18421 tggagtttat acaatgctct tctcttacca atttttgaaa tatttaaata ataaagaaat
    18481 tactctattc caaataagat agtacccaag tatttttcac ttttttctaa tgttgatgct
    18541 cataactccg ttccaagtca cacacctttc gcttatgggt ttggtgagtt tctaggtcag
    18601 catcaatttc aatggtaata ttttgaaaac cacaatcttt taggaaaatt cgaatagact
    18661 ctttacaagt ttccatatgt tccatttctt ttagacaaac atggacaatg gcattttttt
    18721 ccaaagcatc catagtccag agattaagct gattaaggct ggccacattg tccaatcgct
    18781 ccaggtcact ctttacttgc ttgatatcaa gaccttctgg cacagcatcc aagaaaatct
    18841 tgagtgtaga ccaaaaacgt ggaagggctt ttgaaagaat aaagaaagaa atgacaaggg
    18901 acaaaagagg atctaggata taccagtccg taaatcgaag aacaatcgcc atcaggataa
    18961 cagctaccca ccctagcgta tcttccagaa aatgcagact cagaatagac tcattctttg
    19021 tctttccctt accaaccacc agactcgcta acagattgat agtaatcgca ataattccta
    19081 accagagaat cccctcatca ttgactggtt gcggatgcaa aatcttcgtg acattttcca
    19141 aaatgactag aacagagcct gttacgagaa tcacagctgt taccacggct cctagcaggc
    19201 taaaccgctt atagcccaag gtgtactgat tgtcttcttc acgattggag attgtttcta
    19261 gaaaagctga tattccaatt gcaatcacat ctcccaagtc atgcacagag tcagcaagaa
    19321 cagcgctaga accaaatact ccacctgcaa taaactcaac aatggcataa gtcaaattta
    19381 agaaaaaagc cacccaaaca gcatattttg ccttcatatt tctcattcct ttgttataat
    19441 agatttatga acaccttgtt cattatcatt atccactaaa actcaaagaa aggatagaag
    19501 caaaacgtca gctttattca gttctgaaca atttgcctca agtgtccata tgactaacat
    19561 tgaccgccgt atcagcaaaa ccaaaaaagc catctatcaa gcttttatac aacttttgaa
    19621 tgctaaggac tacgagacca ctactgttca ggatatcatt gatctcgcag atgtgggacg
    19681 atccaccttt tactgtcact atgagagtaa agagcttctt ctggatcagc tctgccgcta
    19741 cctcttccat catctttttg aaagagagca agccatttca accgaggact acctcgccca
    19801 cctctttctc cattttcaga aaaaccaaga ccatatcgcc agtctgctat tttccaaaaa
    19861 tgactatttc ctccgtcaac tacagagaga gcttgagcac cacgtctatc ccatgctggc
    19921 tgctgatttg aagcaaactc acccaagtct acctacttct tacctccaac attttgtcgt
    19981 aactaacttt atcgaaacat tgacctggtg gctcaaaaaa ggtcaagatt tcacagacca
    20041 ggaagttgtc caattttatc tagaccttct cattcctaaa aattgaatat agagtaaagc
    20101 ttcagttgtc ttatttctag gttactgagt tttttatctt ttcaacaaca aaagaggacc
    20161 cgccgatcct ctttttcata ctataaatcc ttgattatca actatatctg ttttaatcga
    20221 aatctcaaaa cagcactttc aaacatcttt tcctagttaa gtaaatcagt attctgctta
    20281 gctgccttgc tccattgata ccaaccaact agactgttaa tgagataaat tagatatttc
    20341 ccttgaattt gcaggctttc tccccaccag agatagattg aaaagacatt ggtagccgcc
    20401 cagaatatcc actgttcacg gtaaacagct gtcatgagga tttgccctac cccattggtt
    20461 gcatctgtga ttgaatcacg atagggacga ttggcaccaa tagactgata aatgaagcca
    20521 aaggccaacc accaaagcac actaatggaa agatactttg tccagccctt gccgtccagt
    20581 ttacgcgcga caaactcctg cttttccttc ttaaactgtg cctgataaat ccaaactaga
    20641 agtccaattg gctgcatgac tgtgaagtaa agtgtcgtca gcacctcacc ataaaagcct
    20701 ttctgtaggg ccaaaataag gtaaataaca gagttaatca agccaaaaag ataattactt
    20761 gctcgacctt ccgatacaaa gattacacag ataatccctg tcaagctaca aatcatccca
    20821 atccagtcaa caatacgatg ttcgtaaacc aactccagcc agagaggaaa acttcctaaa
    20881 accagcaaat aaatccactg ggcaaaacta cgatgggcaa agaggtcatc ccagatagcc
    20941 ttcatagttc ctgaaaatcc taaatcagcc atagccgcaa ccatacgacg gtaaccacct
    21001 gacatttcac ctagggttgt tttgatattt tcaattttct tttgcaaata agtatgcatc
    21061 atttctcctt ttgtttttaa agagccgtgt ctggatagac tttcggacgc aacgctctat
    21121 tagataatga actgcctata cacaagattt ctaaccttag tcgacatgag ctgaaacccc
    21181 ttatttgtta agtagttcac aaaatattat acacctattt tatgaatagt caactgtctt
    21241 tacagtaaaa ttttagaaaa tcatgaaaat tttctctttc tttccatttt aagtgacatt
    21301 cagtcattct cacatcaaaa aagcccagac gaaattgtct gagcattctt ttatctagtc
    21361 gtttaaggaa gttgagttca gtatgtttaa agtctctgtc ccatcatttc ttcaacaaac
    21421 cttgttcttg gagaaactcc ttggctactt gctttgctga cttgccttca acaccgactt
    21481 ggtagttgag ctggctcatc tggctttctg taatcttacc agccaatgta ttaagaactc
    21541 tttccaactc tgggtgtttc ttgagaagag cttctttcat gagtggagcc ccttgataag
    21601 gtgggaagag ttgcttgtca tcttccaaga cctgtaaatc ataacgctcc aattccgcat
    21661 cagtcgaata ggcgtccgta atttgaatat cacctgactg aatagcctga tagcgaaggg
    21721 ctggctccat ggtcgctaca ttaagattga gaccatacat tgattgcaag cccttatttc
    21781 catcttcacg gtcgttaaac tcgagtgtaa aacctgcctt caactgccct tccacttttt
    21841 tcaagtctga aatggtcttc aagccatatt cttgagcaat ctttttcgaa acagctacag
    21901 cataggtgtt ttgataagac atgggtttga gataggctag atgatcctgc ttagcaatgc
    21961 catcacgcgc cacctgataa acctgttctg gttcatgact caccttgggt gatggttgaa
    22021 gcaaactttc agtcaccgta ccagtaaatt caggatagat gtcaatatcg ccttttttca
    22081 gagcttcata aaggaagctt gtcttcccaa aattcggttt aacagtcgca gtcatgctgg
    22141 tattttcttc aatcagcaac ttatacatat tggccaaaat ttctggttct ggacctattt
    22201 tcccagcaat aaccaagttt tccttctctt tttgaaccaa aagagctgga ctataagaca
    22261 gacccagtaa taaagccacc aaggcaaaac ctgagaaaat cgtccgtaat tttgcttttt
    22321 ccattacttt tagtaggaag ttaaaggcga tagcaagcac tgcagaagaa agtgccccaa
    22381 tcaaaatcaa actggcatta ttacggtcaa ttcccaacag gataaaggaa cccagtcccc
    22441 ctgctccaat caaggccgcc aaggttgccg taccgataat caaaacagct gccgtccgaa
    22501 tcccagacat gataacaggc atggcgagtg gaatttcaaa tttcttgaga cgttcccatc
    22561 tggtcatccc aaaggcaatc ccagcctctt gcaggttcgg atcaattccc ttcagcccag
    22621 tgatagtatt ttgcaaaata gggaaaatcg cataaatcac tagagctgtc aaagccggca
    22681 aggtcccaat tcccatcaaa gggataaaga gccccaacaa ggccagagac gggatggtct
    22741 ggaaaatacc ggcaatctgc aagacccagt cggccagctt ctcatgatag cgaagaaaaa
    22801 cagccaaggg aatcgcaagc aaaatagcta gtaacaaggt caaaagtgac aactgcaaat
    22861 gttgagatag agctgtcaac caatcactaa aacgatcctg aaaagttgca attaaattag
    22921 tcatgaacac tacctccaaa caagtctgct acaaagtctg ttgcaggcac ttttaaaatt
    22981 gtctcgggat tcgctacctg gcgaatttct ccatcctgca agacagcaat acggtccgcc
    23041 aacttcaagg cttcatccgt atcatgggtt acaaaaatcg ttgtcatccc aaactcttta
    23101 tgcaattctt ttgtcagaac ctgcaactgt tttctcgaaa tagcatccaa ggccgaaaag
    23161 ggttcatcca tgaggaaaat cttgggctga ccaatcatag ctcggacaat accgacccgt
    23221 tgctgttctc caccagataa ttcactaggt aagcgatgcc catactcggc tactggtaaa
    23281 ccaaccttag ccaaaagctc ttctgttttc ttcgtaattt cttccttgct ccaccccttc
    23341 atttcaggaa tgagagcaat attttccgca actgttagat ttggaaaaag agcaatagcc
    23401 tgtaaaacat aaccagtaga aagacgaagt tcacgctcat catagtcttt gatgcgcttc
    23461 ccatccatat aaatatttcc atcagttggt tccaaaagac ggttaatcat cttgagcatg
    23521 gtcgtcttac ctgacccaga aggccctact aaaaccataa attccccatc ctcaatctgt
    23581 aagttgacat ctctcaagac atccttttct gtgtagcgca gtgctacatt tttgtattca
    23641 atcattcttt gtcctcaatt taaaacttcc ctcgattggt caagtcttct accttaggca
    23701 taacttcctt attatcccaa tgctccacaa ttttcccgtt ctctaaacgg aagatatcgt
    23761 actgggcata agcaacgcca tcaatctgag tctgaccata gctaaccaca tagtttcctt
    23821 gtcctaagag ttggaaaaca aagtcaaaag tgacactata ttcagccaca tagtttttat
    23881 aagcagcact tccttgtcca atatcatgat tatgctgaat caaatcgtct gccacataat
    23941 cactccactg ctctagctcc ccattttgga aaatttctgt caagaaacgg cgaaccagct
    24001 ttttattttc tgctttctta tccaaatcct tgatttcaaa atctccaaaa atttgatcta
    24061 gttggtcatt ttcaggtgtt cgatagtagt caatgacatc ccaatgctca acaatacaac
    24121 cattctcatc ctcacggaaa gtatccgtcg tcacccattg agcttctcca ccattcagat
    24181 attgatgaac atgaacaaag accagattgc catcctcaat ggtgcggaca atcttaatct
    24241 gacgctctgg atgacgctca aagaaatctg caaagaaggc tgcaaatcct tctttcccgt
    24301 caggaacacc tgtcgaatgt tggatatagg tatcccctac agactgggct tgagcctcag
    24361 caactcgtcc gtcttgaatg gcatggatgt ataggttgtg agcatttttc acttgttgtg
    24421 acatattcta aacctcattt cccttctctt tcagattcgc caaaattctt tcttgaaaac
    24481 cttcaaattg gtgaatttct tcctctgaaa atcctttgta aaagatagta tccaatttct
    24541 gactgacacg atgccccact tctttctggg acttgcctaa ctccgttaaa actaaatact
    24601 tcttacgctt gtcttttcca cacggactaa caattacaag cttttgttcc tctagctttt
    24661 ttatcatagt cgtcagcgta ttattcgcaa gtccagtcgc aagcgcgata tctgtcgcag
    24721 ttgcgcagcc agtttcacta ttccataaaa ccgctaaaat cttgccctgt tcacccctat
    24781 aaagagcctc aggatcttga ctcagtaact tttgaaaaat ccgcccattc aacaaacgaa
    24841 tatgatgggc tagcaaatga ccatctttca taacacctcc aatttatttc gatatcgaaa
    24901 tgaataaaac aattgtaaca ctcatcgttc taactgtcaa ctatttcgat ttagaaataa
    24961 tttttgataa ttatccacac caccatactc aggctcaact aacttttaac gagagtttct
    25021 aaactccttc gtcctccagt ctacaaaagc cttccattcg tactatccta tattttatga
    25081 ggggacacat ttttcctatc agaccattta ttttaaagat agaagtaaat cataattgct
    25141 tccatctgtt cttttatagt atattgaagt tagactagag cactgtatct tctaaaacat
    25201 tgatagaaag cgatttgaat ttcccaatca atttgttcgt atttatagca tttcgaaact
    25261 ggaataggac accatgactg ctaaaagatt tctataaatt catttaattt cctcaatcaa
    25321 tttgttcata tcttatttca ttccgctata atttcacctt accctatctt tttcgtagca
    25381 cccttcaaac agcctatccc ctaccgtttg acgattcctc acttcgctcc acttccatta
    25441 cagaagtttc ttcactacta tgggctcggc tgacttctca tgattccttg ttactactat
    25501 ttgaacgctc acgagataga tcttacaaaa aatgctttga tccacaatgg aatcaaagca
    25561 ttttaaagag ttcctcatac ataagcgcag aagtcgcagt tcctctgtac ttggcttctt
    25621 ctcttttgac aaagcgagcc aagttgagca actcaggtgc tggatgtttg ggatttagga
    25681 gcaattcacg attgaccagg cctgagagac gaactgcctg caattgctca tttgtagtag
    25741 gcagtttttt agtagtctct aggagagcag caactaaatc ttcactcaaa tcatgtcgag
    25801 catgattgta aagatctttt ataaggcttt ctaggtttgg ttctaccatc cctaccacct
    25861 cccttatggt ttaataatgt ttaatcaaat caaccgttga acgatccaat ttcttcacca
    25921 aggcttgtaa gaaagcttgc gcttctagga agtcatccat tgcatagagg gtttggtgag
    25981 aatggatata acgagcgcag acaccgatag ttgttgatgg gacaccacca tttttcagat
    26041 gagctgcacc tgcatctgtt ccgcctttac cacagtagta ttggtacttg ataccagctt
    26101 cttcagccgt tgtcaaaagg aaatccttca tccctgggag aagcaagtga cctggatcat
    26161 agaaacgaat caaggttcca tctccaatct tgccttgacc accgtagaca tcacctgctg
    26221 gtgagcaatc aactgcgagg aagacttctg ggtcaaactt ggttgtagag gtatgagcgc
    26281 cacgcagacc aacttcttct tggacgttag aacccagata gagttcattg ccgagttttt
    26341 gacccgataa agcttcagct agctcgctta ccatgaggac accgtagcgg ttatcccaag
    26401 cttttgagat gatatttttt tcattggctg tcaaaattgc agaactatct ggtacaatgg
    26461 tatcaccagg acggatgcca aaactttctg cctcagcctt gtccgcaaaa ccaccatcaa
    26521 aaacgatatc ggcaatggct ggcatggttg gtcccccctt tccacgagtc aaatgcggag
    26581 gaacagaacc tgaaatcaca ggaatttcat gaccatcacg agtcaagagt ttgaaacgtt
    26641 ggctgctaac caccatgggg ttccagccac cgatttctac gacacggaag gtaccatctg
    26701 gcttgatttc gctgaccata aaaccaactt cgtccatatg agaagcgacc aagacgcgcg
    26761 gtgcatccac agcttctgaa tgtttgatac caaaaatacc acccaagcca tctgtcacca
    26821 cttcatccac atgcggtgtc aacttttcac gaagataagc acggacaggc gcttcatgac
    26881 ctgagactgc agcaagttct gttacttctt taatttttga aaataatgtt gtcatttcag
    26941 ttccttcttt ctttcatcca ttttaccact ttttatagga gaaggatagt gggaaggtgg
    27001 atttctaagt tagtatctta gtcctgctct atcttagaaa aggatagtat tctcttgcat
    27061 gtagtgcaaa atctagtaaa cattccaaaa ttaactcgaa tatttatttc caaacaaaaa
    27121 aacaatacac catcaaagtt gtttggattt ttcatgaaat ttacagaaaa tagttgactt
    27181 ccctttcttc tttctttaaa tatatagttg gttgagtttg gaatagtacg ctgtagctgc
    27241 taaaacattt ctagaaatta atttgacttt cctaatagag ttgttcatat cttatttcaa
    27301 tttactatag tacaaaacta gaaaaggaaa aaatcatgac caaaacaatt cttgttacag
    27361 gtgggactag ctacattggc tcccataccg ttaaagctct tttaaatgct ggctatcagg
    27421 tgcatgtcct cgataatctc tccacaggaa atcgagcggc tgtggatagt cgtgctagct
    27481 ttaaagaact ggatgtttat gatgctagcg ccttaaaggc ttacttagaa gaaaatcaga
    27541 ttgatgctgt cctccattgt gcaggtgaaa ttgttgtgag cgaaagtatt gaaaatccaa
    27601 gtaaatactt cactgccaac gttgctggta tgaaccaagt tctcaaagtc ttatctgaag
    27661 ttggcattca aaaaatcatg ttctcttcga ctgcttctct ctatggaaat aactgtattg
    27721 acaagccggt gactgaagat accctgctcg accctgtcaa cccttatgca gagacaaaac
    27781 tgatgggcga acgaatgatt tactggatgg ccaatcgcta cgactggaaa tatgttattt
    27841 tccgttactt taatgttgct ggggctgaaa tggatgcttc aaacggtctg cgtgtgaaaa
    27901 atccaactca catcattcca aatatcaaca agaccgcatt gggacaaaat gatagcttaa
    27961 aaatatttgg agatgactac gatacacgtg acggttcatg tattcgagat tacatttatg
    28021 tcttggacct tgcataggct catgttaaag gaatgaacta cctatttcaa gaagacagtt
    28081 cttctcaaat ctttaactta ggaactaaaa aaggctatac tatcaaagaa atctttaaaa
    28141 ctgctgaaga attactaaat taaaaaatcc cacacgagat tgttgctcga cgtgctagtg
    28201 acccagccag tgtcctagca gatgcttcaa aagctaaaca atatcttgat tggacggcta
    28261 gctactccct cgaagatatt attttatcag attaaggtaa aaacatttta gaataggata
    28321 acaaaggaga aagtcttgtt gctctctcct tgttgtattt attaaattgt cagaaatttt
    28381 attgacagat tagaaaaata atgttacatt tatatccgca gatatctttc gataccaaat
    28441 ctacatgaaa ggacggggta tgaaactttc tcattattta attggcttac ttctactcct
    28501 agtctttctc tctattagca ttgggaccag tgatttttca tggggaaagc tatttgattt
    28561 cgaccagcag acctggctcc tctttcaaga gtcccgtctc ccaagaacta tcagtattct
    28621 cctgactgcc tctagtatga gtatggcagg ccttctcatg cagactatta cccaaaatca
    28681 gtttgctgca ccgagtacag ttggaacgac tgaagccgcc aaactgggaa tggtgctgag
    28741 cctttttgtc tttccatcgg ctagtctgac ccacaagatg ctcttcgctt ttgtttcatc
    28801 catcgtattc accctcttct tcctagcctt tatgaccatt tttactgtaa aggaaaggtg
    28861 gatgttgcct ctgattggga tcatctatag cgggattatc ggctcagtca cagaagttat
    28921 cgcctatcgt ttcaatctgg ttcagagtat gactgcctgg acccagggct ccttctccat
    28981 gattcagacc catcagtatg agtggctctt cttaggcctc atcatcctga taaccgtttg
    29041 gaaattatcc caaaccttca ccatcatgaa tctagggaaa gaaaccagcg agagtttggg
    29101 gatttcctac tccctacttg aaaaactggc cctctttctg gtggcgctaa cgacaagcgt
    29161 caccatgatt accgtgggtg gcctaccatt tctcggagtt atcgttccca atcttgttcg
    29221 caagcgctat ggagataatc taagtcaaac caaactcatg gtcgcactgg ttggtgccaa
    29281 tctagttctg gcttgcgata tcctatcccg agttctgatt aggccctatg agttgtctgt
    29341 cagtctcttg ctaggaatca tcggtagtct cgtctttatc ctacttctct ggagaggggg
    29401 acgaaaagat gcagactaaa agcaaacata ccaagctctt ctggattctc attattcttg
    29461 ccatcggagc ttgtcttctc tacttttggc ccatcactca cttgtcagcc tttgcttgga
    29521 agttgcgttc ccaaaagatc atcgtttatc tcttggtagc catcgcgact gggatttcga
    29581 ccattagttt tcaaaccctg acggaaaatc gcttcctgac gcctagtatt ttgggaattg
    29641 aatccttcta cgtcctacta caaaccctac tactggtttt tgaaagcaag tttcttcaac
    29701 ttggcaaatc ccctatctta gaattcctag tcttacttct tgtccagtcc ctcttctttc
    29761 tcgccttaca aggttacttg aagacactga tgaagcaaga cctggtcttc gtcctgctga
    29821 tctgtctagc gctcagaagt ctctttcgaa atatcagcac cttccttcaa gtcctaatgg
    29881 atccaaacga atacgataaa ctgcaaaata gtctttttgc ctcctttcaa catctcaaca
    29941 cttccatcct agccatcggt tctctgatca tcctcgcttt gacaatcttt ttctttcgaa
    30001 aagcagtcgt tctagatgtc ttgcacctgc aaagagaaac ggctcagata ttgggactcg
    30061 atgttgaaaa agaacagaaa gagctcctct ggggaatcgt gcttttgacc tcaacggcca
    30121 ctgccttggt aggacctatg gccttcttcg gctttatact ggccaacctc acctacctga
    30181 ttgtcaaaga ctatcagcac aagttactct ttatagtggc cattctggtt ggatttatta
    30241 gcttaacctt ggggcaagcc ttgattgaac gagtctttgc actggaaatt cgtatcagta
    30301 tgatcattga gagtgtgggt ggcttcttat tctttatctt actatatagg aggtctcgtc
    30361 agtgaaactg gaaaacattg acaaatccat tcaaaaacag gatattttgc aaggcatttc
    30421 gcttgaagtc agtcctcaaa aactgacagc ctttattggt ccaaatggtg ctggaaaatc
    30481 gactctcctc tccatcatga gcaggctaac caagaaagat cagggagttc tcagtatcaa
    30541 aggacgtgaa atcgagagct ggaattcgca agaactggcc caagaactaa ccatcctaaa
    30601 acagaaaatc aattaccaag ccaaattgac tgttgaagaa ctggtcagtt ttggacgttt
    30661 tccctacagc cgaggtcgac ttagatcaga agactgggaa aaaatccgag aaactctgaa
    30721 ctatttagaa ctgaccaact taaaagaccg ctacatcaat agcctgtcag gggggcaact
    30781 ccagcgcgtc tttatcgcta tggtactggc ccaggatacg gactttatct tgctggacga
    30841 accactcaac aatctcgata tcaagcaaag cgtcagcatg atgcagattc ttcgacgact
    30901 ggtggaggaa ctcggcaaga ccattatcat cgtcctccac gatatcaaca tggccagtca
    30961 gtatgcagat gaaattgtcg ccttcaagga cggccaggtc tttagcaagg gaagaaccga
    31021 tcaaatcatg caggctgacc tgctcagtca actttatgag attcccatca cgctagctga
    31081 tatcaatgac aaaaagatct gtatctatag ctagtaacat aaaagctcaa gttagagaac
    31141 cttcagtctc ttagtcaata agattaagag actccctaaa tcgttatcac attttaaaaa
    31201 ggagaaatta tgaaaacatc ccttaaactt tatttcactg ccctagtggc cagcttcttg
    31261 ctcctacttg gtgcatgtag tacaaactca agcactagtc agacagagac cagtagctct
    31321 gctccaacag aggtaaccat taaaagttca ctggacgagg tcaaactttc caaagttcct
    31381 gaaaagattg tgacctttga cctcggcgct gcggatacta ttcgcgcttt aggatttgaa
    31441 aaaaatatcg tcggaatgcc tacaaaaact gttccgactt atctaaaaga cctagtggga
    31501 actgtcaaaa atgttggttc tatgaaagaa cctgatttag aagctatcgc cgcccttgag
    31561 cctgatttga ttatcgcttc gccacgtaca caaaaattcg tagacaaatt caaagaaatc
    31621 gccccaaccg ttctcttcca agcaagcaag gacgactact ggacttctac caaggctaat
    31681 atcgaatcct tagcaagtgc cttcggcgaa actggtacac agaaagccaa ggaagaattg
    31741 accaagctag acaagagcat ccaagaagtc gctactaaaa atgaaagctc tgacaaaaaa
    31801 gcccttgcga tcctccttaa tgaaggaaaa atggcagcct ttggtgccaa atctcgtttc
    31861 tctttcttgt accaaacctt gaaattcaaa ccaactgata caaaatttga agactcacgc
    31921 cacggacaag aagtcagctt tgaaagtgtc aaagaaatca accctgacat cctctttgtc
    31981 atcaaccgta cccttgccat cggtggggac aactctagca acgacggtgt cctagaaaat
    32041 gcccttatcg ctgaaacacc tgctgctaaa aatggtaaga ttatccaact aacaccagac
    32101 ctctggtatc taagcggagg cggacttgaa tcaacaaaac tcatgattga agacatacaa
    32161 aaagctttga aataagccac ttcaaactca atttaatact caatgaaaat caaagagcaa
    32221 actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgcaga tgaaagctga
    32281 cgcggtttga agagattttc gaagagtata aaattttgac attctaaaac gcatcctatc
    32341 aagttcactc aaacctgata ggatgcgttt ttatcatttt gaaatctatt taccccacct
    32401 cattcccctt cttgatttcg tttaagggaa aagcggtctg gaacggggtc cttacctgtt
    32461 ttcgaccagg gatgacaacg taaaatccga gccaagccca tcaatacccc cttaaaccca
    32521 tgtttttcaa tagcctgaat catgtagttg gaacaagtca gctcaaagcg acaagagggt
    32581 ggaaagactg gtgagataaa acgttggtaa aagcgcacag gcgctattaa aattcgtttc
    32641 attatttctt agttacagcc atggtgtgta gttggctgat ttctttttta ttaagacgac
    32701 gggattctcc tggacggagt cctgtcaagt ctaggtgtcc gaaacgagtc cgagacaact
    32761 tatctacttg gagaccaaca gcttcaaaca tctttttaac ctgatggtta cgcccttcat
    32821 ggatggtcaa ctgcaccaca gagcgatttt tgactgggtc cactttgaga atttcataaa
    32881 cagctggctt ggttttctta ccatcaatct caagaccacg ggtcaagggg cggagattgt
    32941 ccttattggc cacaccttta acacgcgcga cataaacctt gtcaatctca ttacgagggt
    33001 gaatcatctc gtctgtaaag tccccatcat tggtcaaaat caagacacct gatgtatccc
    33061 agtccaaacg acccacaggg taaatacgct ctttgacatt gggcaagagg tcgacaaccg
    33121 tcttgcgacc cttatcatct gtcacactgg aaatcacacc gcgtggtttg ttaagcagat
    33181 agtagacctt ttcttcgttg tagataggtt gaccttcaac ttcgaccttg tcgcctgact
    33241 tgatagtggt tgctagttca cgcaccactt ggccgttaac cgtcaccaag ccttgcttaa
    33301 tcagctcttc tgcttttctc ctactggcca cacctgcgtg ggcaatatac ttattgattc
    33361 tcatcttctt ctatcctttc accaaataat tggctttctt gggcttgaat ctcaagctca
    33421 tcaatcactg gtaattcttc taaatggttt atccccatgt aatctaggaa ataatccgta
    33481 gtcacataga ggttggggcg ccccaatact tcctttttcc cgtcttcctt tatcaggtca
    33541 aaagcctgca actttgccaa ggctccactc gagttaactc cacggatggc atcaatttct
    33601 atccgcgtaa tcggctgttt gtaggcaata atggacaagg tctcaagggc agcccgagac
    33661 aagctctggt tgataggcgc cttagagtat tccttcaaaa tctctgcaaa ttgaggcttg
    33721 gtcaccaatc tataagcacc acttgtctca atcaaagcca aactggaatc tgggtccttt
    33781 tcatacttct gggctaattt tcctaaactt tgctggatgc ctgtcggtgg cagagagagg
    33841 agttcagcta actggcggac ccgaatccca tcttcacccg ctacaaacaa gagcgcttct
    33901 atttttgcta aagtactcat ctttcctctc tatcaagtct agctttgagg cacttgactt
    33961 tcttccttct tttccatgag atagatatct ccaaaactct cctcttgcac gaggatcaac
    34021 tcctgggttt tgattaactc tagggttgcc aaaaagaggg tgatgacctc ttggacattc
    34081 tgggcttcct tgaacaaatc ctgcaagcgc aattgatctc gtccaatcaa ggactctttc
    34141 acgataatca tcatgtcctc aatcttatac tcatcccgca agatcgtcgt gtgattttgt
    34201 gcaaactcct cttttttctt ggctaggata tttgaaaaag tcaaaaagag gtcaatggtc
    34261 gtcttgtcat gcacaagctc cgcatcttcg taaatcaact ctgtcggcgc tttggaataa
    34321 tactgggccc gttcttggtg cttggcttcc aagtgctcac ccaagagctt gaacttgcga
    34381 tattcttcga tttgagagag gaggtcctgc tccaggtcat cccccaagtc tgtcacttct
    34441 gctaccttcg gaaggagttt acgactctta atcagcatga gctgactagc catgaccatg
    34501 tactcacccg tcacttccag acgcatggcc tgcagggttg agacataggc tagatactgt
    34561 tcgatgactt ccgtaatggg cacatcgtag atatccatct ggtacttaga aaccagatgc
    34621 aagagcaagt ccaggggtcc ttcaaaatct tttaatttaa tatccattat ctatattttt
    34681 ctaaggtcag gactgttttt aatcctaatt tttttgcaat ttcgtacaaa tcgaccttgt
    34741 tttctatttg tcttagaata aactgttcac gtaagacttg agctgataag gatggaaaac
    34801 ctttctcctt gacaaaagat tctaactgac gaaaggccca ctgacgagaa tagggtttct
    34861 ctcctctttc aaaaagatag gtctgcccca tcaagggttc caattctgaa agcaaggccg
    34921 tgggaatggt gacaatcctc tgttgggaag ccttgctgat tcgcagcacc tgaaaatcca
    34981 gattgatgtc cgcaaccttg atggctaaaa tctcactggg caagagcccc atttctagga
    35041 ttaagagcgc tagcaagcgg ccctctggat ggtcgctttc ctgccaaaaa gagtctaggt
    35101 atagaatctc tggcttttcc gtcttctttt cagcttgttt ggctaattcc aagcggtaaa
    35161 agctgtccac ctctcctttt tgatagagaa agtatagaaa ttggttacag gccgaaatct
    35221 ttcgcttctg ggcgctgatt tttagattgg ctagctgggc ttggtaaatc ttgagactgg
    35281 tctcagaaat ccgctcacct accatgtcta aaaattgctc caaatcatac ttataggact
    35341 gcttggaatt gacagacaag ccctgctttt cctctaaaaa ggctgaaatc ctgtctctca
    35401 ttggcatcga atctcatatt ccttactaaa gtcatgcaag agggcattaa cggccttgag
    35461 gatggacttg cgcgtaataa tcccttggaa aataccctct gcatccacga ccggtaagaa
    35521 ggactcatct actagcttgt gcaagacctc cgtaatggtg aaatcaggcg aaacaaccgc
    35581 tacgtccgtt tttgtcatat gaacgatatc cgtatccgcc atgatttctt ggctcaagtc
    35641 atgctccatc tgataagcca taatatctct gagtccaatc gtcccaacaa actgtttttc
    35701 atctgtcaca acgggaacac gggtataggt catctgactg agcaagaggg tcgcatgatc
    35761 cgcattgtgg gtatcaatca acacagctag atttttagca ggggtcaaaa aagtttcctc
    35821 ctgccccaac aagaaagtct caaactcctt ggcaatcatc ggctaaactc cttggacaaa
    35881 cctggataca cctcgtgatc tcgtgtcaaa aagtccactt tgaagtaact atcatcaatc
    35941 tccacacgag catagagaca ttctctgatg gtacctcgtg gttgactgat agaacctgga
    36001 tttagaaaga ggatcttgcc ttccaaccaa gcacttggca catgcaagtg accatagagg
    36061 cagatagcgg cctcttcctc ctgagcccag tagtccaact tttgaaagtt gaaattgatg
    36121 tcaaacaagt gaccatgagt ttggataatc ttggtcgaac caagctcagt caccagacgt
    36181 tctgggtagc cggcgtagaa gtccatgttc cctttaacaa cgcggatgcc ctcccaaagt
    36241 ggagaatccg gacgtagttc agaatcgccg ttatgaaaaa cggcatcgac tttgcccaca
    36301 tagcgatcac ggacttcttc cacaatcaag ctatcgccat gggaatcgct cattacaatg
    36361 atggtttgct ttgccatgat ggaaatacct ccaaaagttt cttaacggct aaggcacggt
    36421 gagattgact atttttttct tctagggtta attcagctga tgactcacct gtctctccta
    36481 caaggaagag gggatcatag ccaaagccat tttcaccctt aggttcaaag ttaatataac
    36541 ctgaccagtc tgcttcaaca actaaacttt ccttatttgg gctggctacg actagggttg
    36601 tgtggaactg agccgagcgg tccttgagtt caaagaccat ggccaattcg tgcaagagtt
    36661 tggcattatt ttcacggtca gttgctccca cacctgcgaa acgagctgac cagacgcctg
    36721 gtaagccacc aaggacatcg actttgagac cagaatcatc tgccaaaacc atcttacccg
    36781 ttaattgaga aatggtttct gccttaaggc gggcattttc ttcaaaggtc atacctgttt
    36841 ctgctacttc aggcaggtca gggtagtcat taagattttc cacatcgtag cctaacttat
    36901 caaagatagc tcggaattcc ttggttttgc cttcgttacg agtcgcaatc aacaaggttt
    36961 ctctgacctt gcttgtctca aagaaatcat gaacattgac cccttcttta ggcaattcta
    37021 catacaagag ttgcccattt tcaaccaaga gcagggcccc atgacgttga ataacttcca
    37081 agtttcgtcc catttcttga ttaagcatat ctaccacaaa ggagaacaaa cggtaggtag
    37141 aaccatagcg taaaacagta actgaaagcg ggaaaccata ctcttcatct ccaaatattt
    37201 tggagaattc aaacagagga aaatctgtct tatagtcgct caaactgtta acgccaccaa
    37261 aaatactata agacccaaca taccagtcct ggtcatcctt atattcataa attttatttg
    37321 tcataattct acatgctcca catgaatctc tttttccagc cattcttcac caatttgtgc
    37381 aaaactttgg ctactggctg ttgtgtaaaa acggtgatgg agtggtccag catcgcgacc
    37441 acgattgatt tcaaaataat tgagtaagac tgagatatcc cgtacgcact ctgccccact
    37501 atcgatgagc tgaacctttg gccccatcac attttggata atagggcgaa ggagtggata
    37561 atgagtacag cccaaaatca ggctatccac ctttccaacc aagggacgca gggtttcata
    37621 gaccaccttc ttggtaacac tggttgacag ggcacctgac tcaaccaagg gagcaaactt
    37681 gggacaggcc aagctctcca cctgtaagtc gggatccaga tcatggattt tctgacggta
    37741 tatgtctgat tgtaccgtca tgggcgttcc aatcactccg attttcccac cttgactgga
    37801 cttgatggct gccgaagctc ctggcaaaat tacacccaag acaggaatat ctagttgagc
    37861 cttgatttct tcccagacga ccgcagtcgc agtgttacaa gcaatgacaa tcattttgac
    37921 atccttggtc aagagaaagt tgaccagctg ccaagtatat tcacgaattt gctcagcagg
    37981 acgggggcca tagggcgccc gcgccgaatc tccaatatag acgatttctt catggggaag
    38041 ctggcgcatg agctcgcgca caacggtcaa gcccccgaca cccgaatcca aaaaaccaat
    38101 tggtcgatta tccatacagt cttctttcta gtcttttttc tgattgatcg ttcattatga
    38161 ttccttgtac tgaacgaact gaccgacagc ttgatagaat gtcaactacc tctctcttaa
    38221 tcataatcaa atatcaatag aatgcaagga aaaagtctca tccctaaacc ataaccaaca
    38281 tagtgagaag tctggaactt tcatcccaga cttttcctta tttatttttt ctttttattg
    38341 ttagcaaggg ctgccttttg ttggcggatg atttggtggt aaacttgttg taccttagct
    38401 tcgcttggtt tttgaccatt tgcactcaaa agagtacgaa ctgcttcagc attcaaacgt
    38461 gggttgtcag caaattcttt ttcgatttgc ttacgaacca agtacattcc tccaagagct
    38521 cctcctagaa aagctagcac aatcaataca attgctaaaa gtaaatccat catttttctc
    38581 ctgtttcttt ttgagtccct ttcattatac cacaaactag ccaccctgac cagtttgttc
    38641 tacactttca aggatggaat agacagcaaa aagaaccctg cttttctgaa aaagaggtct
    38701 ccttgctaag tttatactca atgaaaatca aagagcaaac taggaagcta gccacaggtt
    38761 gctcaaagca ccgctttgag gttgcagata aaactgacac ggtttgaaga gattttcgaa
    38821 gagtattaat ttacataaat agccagtgtt tgatagggtt tgagtagaat tttctcagac
    38881 acttctgcat cttcatagtt tgaaatcaaa atctgtccat tttggtagac tgctggcaag
    38941 tcgatttcca cttctttagc ataaaagtta ttgagcacta gtaacttttg atcctcaaac
    39001 tggcgttcaa aagcgtagac ttgtttgcta tcttcaaagg ctggtttgta acttccttct
    39061 gaaatgattg gcatttcctt acgcagtcga atcaagtctt gatagaaggt aaaaatcgga
    39121 ccctggattt cattttctac attgatgtct ttataggatt taccagcttt caaccaagga
    39181 gtgcctgttg aaaatcctgc attttccgaa gcatcccact gcatgggaat gcgtgaatta
    39241 tcacgcgact tagcttgaat aatctggaag gcttcttgct gactctttcc ttcttctaag
    39301 agcatctgat aggcattaag cgattcgaca tccacataat cagccataga atcatagtct
    39361 gggtcaatca tcccgatttc ctcacccatg tagatataag gtgtcccacg tgacaggtga
    39421 atgctggccg ctagcatggt ggctccttcc ttgcggaagt tttgaatatc gacaaaacgg
    39481 ttcaaggcac gtggttgatc gtgattgttc caaaagaggg cactccaacc gtctttatca
    39541 ctcatttcct taccccaact atggtaaaga ctcttcaact cttcaaaatc aaagggagcc
    39601 aaggtccact tttgtccatc cttatagtcc accttgaggt gatgaaaatt aaaggtcatg
    39661 gataattcct gacgatcagg cgacgaatag aggacacagt tttccatggt ggtagaagac
    39721 atttccccaa ctgtcataaa gctatcgtca gatccaaaag tggcttggtt catcatacgc
    39781 aaatagttat gaacgatggg tttgtctgta taagctggct tcccttcatt ttcaggacag
    39841 tccactgaaa cctcgtcctt accgatcaaa ttgatcacat caaatcggaa acctttgaca
    39901 cccttgtcgc gccagaaatt aacaaccttg aaaagctcct tacggacatt ggaattgcgc
    39961 cagttaaggt cagcctgggt ctcatcaaat aggtgaagat agtatttccc agtatccccg
    40021 aaaggcgccc atgcagaacc accaaactta gactgccaat ctgttggttg gtcttggatg
    40081 aagaaaaagt cttgataata cttatcacca gctagggctt tctgaaacca ttcatgctct
    40141 gtcgaacaat gattaagtac catgtccagc ataaagtcaa tcttgtgctc tttaccgaca
    40201 cacaccattt cctcaaaatc agccatatca ccaaaaagag gatccactgc catataatct
    40261 gaaatatcgt aaccattatc ccgttgaggg cttggataga atggattgag ccagaccata
    40321 tccacgccta gtttggctaa atagggaatt ttttcgataa tcccacggaa atccccaata
    40381 ccgttttcag tggtgtcttt gtaagatttt ggatagattt gatagactac ttttccttta
    40441 tcaagtgtca tctgtttctc cttttctgat aaaagggagg aagcagtctt ccgtccctat
    40501 ttgtgctatt tcaattatac tcaatgaaaa tcaaagagca aactaggaag ctagccacag
    40561 gttgctcaaa acactgtttt gaggttgcag atagagctga cgtggtttga agagattttc
    40621 gaagagtatt agattcgtgt agcgaccatg agagatgctc cagcttgaat cgttgtcgga
    40681 taagttccgg gaatagtcgc tgtataagca tcttggttgg tgatgataac aggagtttct
    40741 gtcaccagac ctgcagcctt aatgacatcc atatcaaaac gaatcagttg ctgaccaact
    40801 gtaacgtgat ctccttggac tacaagactt tcaaaacctt tgccatcaag acctactgta
    40861 tccataccga tgtggatgag caattcaact ccctcgtcag agacaatgcc gatggcatgc
    40921 ttggtaggga aaagaaccgt cactgtccca ttaactggag aggtcaactc accttggctt
    40981 ggttcaatga ccagaccttg ccccatgaca cctgatgcaa aaataggatc cgtcgcttga
    41041 ctcaattctt tcacttggcc agttagtggg ctgataattt ctaccgaagt aagttctact
    41101 ggttcatggt tcacaaattc tgcttcttct tgagcaacga attctgcctg caagttcgta
    41161 tcgccctctg tttttgtaaa gagaccagcc ttgcggaaga agaaagtcaa gagcattgga
    41221 acaacaatcg caactagcat agttcctgca aatggcagca tgtattgagg ttgaatagag
    41281 agaatacctg gcaaaccacc gataccaata gaagccgcag ttacattaaa agtaacggat
    41341 aacatgcctg caagggctga accagtcatc ccagcaacaa atggataaat atattttacg
    41401 ttaaccccaa aaagagctgg ttctgtaaca ccgagatagg ctgaaatggt tgcaggaagt
    41461 gaaacctgag cctcacgctc atcatggcga tgcatgaaat aataggcaaa cacggctgag
    41521 ccttgagcaa tattagaaag agcaatcatt ggccataggg cagtgccacc agcatccgca
    41581 atcaattgtg tatcaatggc attggtcata tggtgcagac ctgtgatgac aaatggagcg
    41641 tagagggcac caaaaattgc accgaagagc cctttaactg gaccagttaa acctgccaag
    41701 acaactgatg aaagtccttg tccaattgtc caaccgattg gtcccaaaac agtatgagcc
    41761 aaaatcaagg ctggaatcaa tgacaagaaa ggtacaaaaa tcatagaaat gacttctggg
    41821 atatgcttgc gccagaagat ttcaagataa gacagactca aacctgcaag caaggctggg
    41881 ataacttggg cttggtaacc gatacgatta acagtaaaat agccaaaatt ccaaacccag
    41941 tttgccgcga tatcagctgc tggcgttgaa gcaaccgcat aggcattgag caactgaggc
    42001 gataccaaac agattccgag aacaattccc aaaatttggc tggttcccat cttacgagaa
    42061 acagaccaag taatccctac tggtaagaac tggaagatag cttcaccagg caaccagagg
    42121 aagtgattga cacctgccca aaactgagag gattctgtga tggtcttgcc atccaacatc
    42181 gaccaatgga caccttccaa gacattacgg aaaccgagga tcaatcctcc gactatcaag
    42241 gctggaataa tcggagtaaa aatctccgcc agagtggtca taacacgttg gaccacgttt
    42301 tgattactct tagctgcaga cttggctgct tctttggaaa caccctcaat acctgaaacg
    42361 gctgtaaaat cattataaaa gatgggcacg tcatttccaa tgattacctg aaattgacct
    42421 gcatttgtaa aagttccttt aacagctgga attgactcga tagctttaac attagccttc
    42481 ttatcatctc ctaaaacaaa gcgcatccgt gtcgcacagt gagttacggc agtcacattt
    42541 tctttgcctc caattgcctg aagcagatct ttggcttctt gttcaaattt tcccatttga
    42601 ttctccttat acttgtcaaa gcaagtttga tttgataagt atattgtaaa cgataccaaa
    42661 cttgtctaca acttgtttgg tataaaaagg gtgttttttt gtaaattttc tagcacttgt
    42721 atggtaaaat agatacaggt gttcattaaa ctagactaaa aacctattta agcaggcaaa
    42781 atgaagaaat accaacaatt atttaagcaa atccaagaaa ccattcaaaa cgagacttac
    42841 gctgtcggag atttccttcc tagcgagcac gaccttatgg agcaatatca agtgagtcgt
    42901 gataccgtcc gaaaggccct gtctctcctc caagaggaag gattgatcaa aaagataaga
    42961 gggcaaggtt ctcaagtcgt caaagaagaa accgtcaatt tccctgtatc caacctaacc
    43021 agctaccaag aactagttaa agaacttgga ctgcgctcta aaaccaacgt ggtcagtctg
    43081 gacaagatta ttattgataa aaaatcctca ctgataaccg gtttcccaga gtttcggatg
    43141 gtttggaagg tggtccgcca gcgtgtggtg gatgatctgg tatccgttct ggatacggac
    43201 tatctggata tggaactcat cccaaatctc actcgccaaa ttgctgagca gtctatctat
    43261 tcttatatag aaaatggcct caaactcctt attgattatg ctcagaagga aatcaccatt
    43321 gaccactcaa gcgaccgaga caagattctc atggacattg gcaaagaccc ttatgtcgtt
    43381 tcgattaaat caaaagtcta tctccaagac ggacgccaat ttcagtttac cgaaagtcgc
    43441 cataagttag agaaatttag atttgtagat tttgcaaaac gcaagaaata aaagactgag
    43501 acaccagatc tcagcctttt tcggctctat aatatttgta gtgggtaacc cccctatgga
    43561 tattatggag cctattttgt gtagaaaaaa agtcccatat gacctataat gaaaagcgac
    43621 aaaacaactc attagaaaga ttcatatgga acaattacat tttatcacaa aactgctcga
    43681 tattaaagac ccaaacatca agattctaga catcatcaat atggataccc acaaagaaat
    43741 tatcgctaag ctggattatg aggctccatc ttgccctgat tgtggaagtc taatgaagaa
    43801 atatgacttt caaaaaccgt ctaagatccc ttacctcgaa acaactggta tgcctactag
    43861 aattctcctt agaaagcgtc gtttcaagtg ctatcattgt tctaaaatga tggtcgctga
    43921 aacttctatc gtcaagaaga atca
//
LOCUS       DAVHEW010000007        42249 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVHEW010000007 DAVHEW010000000
VERSION     DAVHEW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.007"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(73..1035)
                     /locus_tag="VVL53_000371"
     CDS             complement(73..1035)
                     /locus_tag="VVL53_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU4182588.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            1101..2489
                     /locus_tag="VVL53_000372"
     CDS             1101..2489
                     /locus_tag="VVL53_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4182589.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(2524..2976)
                     /locus_tag="VVL53_000373"
     CDS             complement(2524..2976)
                     /locus_tag="VVL53_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4182590.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            3138..3432
                     /locus_tag="VVL53_000374"
                     /pseudo
     CDS             3138..3432
                     /locus_tag="VVL53_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3588..4802
                     /locus_tag="VVL53_000375"
     CDS             3588..4802
                     /locus_tag="VVL53_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4182591.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            4947..5081
                     /gene="rpmH"
                     /locus_tag="VVL53_000376"
     CDS             4947..5081
                     /gene="rpmH"
                     /locus_tag="VVL53_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU4182592.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            5365..6258
                     /locus_tag="VVL53_000377"
     CDS             5365..6258
                     /locus_tag="VVL53_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU4182593.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            6563..7336
                     /locus_tag="VVL53_000378"
     CDS             6563..7336
                     /locus_tag="VVL53_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU4182594.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            7336..7896
                     /gene="rnmV"
                     /locus_tag="VVL53_000379"
     CDS             7336..7896
                     /gene="rnmV"
                     /locus_tag="VVL53_000379"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU4182595.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            7968..8867
                     /locus_tag="VVL53_000380"
     CDS             7968..8867
                     /locus_tag="VVL53_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4182596.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            9136..11166
                     /locus_tag="VVL53_000381"
     CDS             9136..11166
                     /locus_tag="VVL53_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4182597.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            11168..11809
                     /locus_tag="VVL53_000382"
     CDS             11168..11809
                     /locus_tag="VVL53_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182598.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            11802..12110
                     /locus_tag="VVL53_000383"
     CDS             11802..12110
                     /locus_tag="VVL53_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182599.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            12241..13113
                     /gene="rsmA"
                     /locus_tag="VVL53_000384"
     CDS             12241..13113
                     /gene="rsmA"
                     /locus_tag="VVL53_000384"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU4182600.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            13115..13993
                     /gene="rsgA"
                     /locus_tag="VVL53_000385"
     CDS             13115..13993
                     /gene="rsgA"
                     /locus_tag="VVL53_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU4182601.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            14004..14660
                     /locus_tag="VVL53_000386"
     CDS             14004..14660
                     /locus_tag="VVL53_000386"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU4182602.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            14623..15285
                     /locus_tag="VVL53_000387"
     CDS             14623..15285
                     /locus_tag="VVL53_000387"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU4182603.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            15287..16543
                     /locus_tag="VVL53_000388"
     CDS             15287..16543
                     /locus_tag="VVL53_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU4182604.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            16533..17474
                     /locus_tag="VVL53_000389"
     CDS             16533..17474
                     /locus_tag="VVL53_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU4182605.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            17741..18568
                     /gene="purR"
                     /locus_tag="VVL53_000390"
     CDS             17741..18568
                     /gene="purR"
                     /locus_tag="VVL53_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU4182606.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            18635..19885
                     /locus_tag="VVL53_000391"
     CDS             18635..19885
                     /locus_tag="VVL53_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU4182607.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYHPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEVVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            20011..20805
                     /gene="pflA"
                     /locus_tag="VVL53_000392"
     CDS             20011..20805
                     /gene="pflA"
                     /locus_tag="VVL53_000392"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU4182608.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(20880..21806)
                     /gene="yidC"
                     /locus_tag="VVL53_000393"
     CDS             complement(20880..21806)
                     /gene="yidC"
                     /locus_tag="VVL53_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4182609.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(21884..22162)
                     /locus_tag="VVL53_000394"
     CDS             complement(21884..22162)
                     /locus_tag="VVL53_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU4182610.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYPDFKIAN"
     gene            22196..22936
                     /locus_tag="VVL53_000395"
     CDS             22196..22936
                     /locus_tag="VVL53_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU4182611.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            22992..23675
                     /locus_tag="VVL53_000396"
     CDS             22992..23675
                     /locus_tag="VVL53_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU4182612.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            23787..24779
                     /locus_tag="VVL53_000397"
     CDS             23787..24779
                     /locus_tag="VVL53_000397"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU4182613.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            24844..25383
                     /gene="rsmD"
                     /locus_tag="VVL53_000398"
     CDS             24844..25383
                     /gene="rsmD"
                     /locus_tag="VVL53_000398"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU4182614.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            25373..25861
                     /gene="coaD"
                     /locus_tag="VVL53_000399"
     CDS             25373..25861
                     /gene="coaD"
                     /locus_tag="VVL53_000399"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU4182615.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDKLVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            25845..26882
                     /locus_tag="VVL53_000400"
     CDS             25845..26882
                     /locus_tag="VVL53_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU4182616.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            26940..27170
                     /locus_tag="VVL53_000401"
     CDS             26940..27170
                     /locus_tag="VVL53_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU4182617.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            27194..28477
                     /gene="murA"
                     /locus_tag="VVL53_000402"
     CDS             27194..28477
                     /gene="murA"
                     /locus_tag="VVL53_000402"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4182618.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            28470..28658
                     /locus_tag="VVL53_000403"
     CDS             28470..28658
                     /locus_tag="VVL53_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU4182619.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            28697..29521
                     /gene="endA"
                     /locus_tag="VVL53_000404"
     CDS             28697..29521
                     /gene="endA"
                     /locus_tag="VVL53_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU4182620.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            29750..31081
                     /locus_tag="VVL53_000405"
     CDS             29750..31081
                     /locus_tag="VVL53_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU4182621.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(31279..31349)
                     /locus_tag="VVL53_000406"
     tRNA            complement(31279..31349)
                     /locus_tag="VVL53_000406"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31315..31317),aa:Cys,seq:gca)
     gene            31765..35376
                     /gene="rpoB"
                     /locus_tag="VVL53_000407"
     CDS             31765..35376
                     /gene="rpoB"
                     /locus_tag="VVL53_000407"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU4182622.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            35409..39086
                     /gene="rpoC"
                     /locus_tag="VVL53_000408"
     CDS             35409..39086
                     /gene="rpoC"
                     /locus_tag="VVL53_000408"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU4182623.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            39197..39610
                     /gene="ndk"
                     /locus_tag="VVL53_000409"
     CDS             39197..39610
                     /gene="ndk"
                     /locus_tag="VVL53_000409"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU4182624.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(40556..41188)
                     /locus_tag="VVL53_000410"
     CDS             complement(40556..41188)
                     /locus_tag="VVL53_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182625.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(41190..41957)
                     /locus_tag="VVL53_000411"
     CDS             complement(41190..41957)
                     /locus_tag="VVL53_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4182626.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
BASE COUNT        12372 a         8168 c         9711 g        11998 t
ORIGIN      
        1 aaactgacga agtcagtaac catacatacg gtaaggcgac gtgacgtggt ttgcagagat
       61 tttcgaagag tattagcctt ccatatagtc tttcaaagcc tgtcctgtta gtccggcatt
      121 gagggcgatg aggagtttca agcgagcttt ttgggcgttg agttctttaa caaagaaaac
      181 gcctgctttt tgcaactgta cgcccccacc ctggtatgca taaacaggct cggcaatacc
      241 gttaaagcat cgtgatacca gagcgactgg aattcctttt tgcagaaggc tttctaattt
      301 ttgagccgtt tctttgggaa tattaccagc tccgaaggct tggataatca aaccgtccaa
      361 gtgttctaaa tccagcatat caatcagctc atctgtcata ccagcataag ccgagatgat
      421 agggactaaa ccttgtatgt gatcaaggtc aaagcgaaca cgaggttcag ctgttttgaa
      481 gtagaggatt tcctgtttca tgatgagacc aagggggcca tgtgttggag tctggaaggt
      541 gctgacatta gtcgtatgtg ttttggtgac atacttggca gcgtggattt catcgttcat
      601 aacgaccaaa actcctttgt cagcagccct gtcatcgctg gccactcgta aagcacttag
      661 gtaattataa acaccatcac taccgagctc attggagcta cgcatggctc ctgttagaac
      721 gataggcata tggggaactt ccatggtatc aaggaaatag gctgtttcct ctaaagtatc
      781 ggttccgtgt gtgatcacca ctccatcgta gttatctgct tcctctttaa ttttctggta
      841 gaggaccagc atatgtttgg gtttgatatg gggacttgga aggttaaaaa agtccaaggc
      901 gtggacttgg attccttcaa gtgggttgga cacatggttc atgggattat ctgaactcgt
      961 cacaacagcg ccagaagcat cggcctgcat ggaaatagtt ccacccgtat gtaaaacaag
     1021 gattttctta ggcatgattt actcactctt tctgaaatgt ggtataatca ttgtatcaca
     1081 aaaggggaga ttgggatagg atggaagtca aagctgtttt ttttgatatc gatggaacct
     1141 tggtcaacaa tcgcaagagt gttttgaaat ccactaagga tgcgattaag attgtcaaag
     1201 aacaaggggt actagttggc gtagcgacag ggcgaggacc tttttttgtt aaggaattga
     1261 tggacgattt ggatctggat tttgcggtaa cctataatgg ccagtatatc tttagtaaag
     1321 acagagtctt gttcacgagc cctatttcca agttacattt gcgccatcta attagctatg
     1381 ctaaaaaaga gggcacggag attgctctag ggaccaagga tgccatgttg ggttctaaaa
     1441 tcatgtcctt tggtctgggt tctttttccc aacgaattag tcgcttcgtt ccctctgttt
     1501 taactcggac ggtgagtcag tcttttaatc gcatggtcag caaggttgtt ccccaaaagg
     1561 aagaggacct gcttcacctg atgaatcagc ctatctacca agttttgatg ctgatgacac
     1621 cagaagaatc tgagaaggcg gcagctgatt ttgaagactt gaaattgaca cgtagcaatc
     1681 cttttgcatc ggatgtcatc aatcagggaa attctaaatt agaaggcatt cgccgagttg
     1741 ggaaagaata tggctttgac ctcaaccaag tcatggcttt tggtgactca gataatgatc
     1801 ttgaaatgct ggctggcgtt ggtatgtcgg tcgctatggg aaatggtagt agcagtgtca
     1861 aggaagctgc caagcacatt accactagca atcaacaaga tggaatccac aaggccttgg
     1921 aacattttgg tgttttgtct tcagaaaaag tctttgtcag ccgtgactat catttcaata
     1981 aggtcaagac cttccaccac atgatggatg aacgaaccca agaagaacct cgagcttggg
     2041 atttagaggg tgcaacccat agggctggtt ttaaaataga agaattggtg gagtttgttc
     2101 gagcagccag tccttctgaa gaagattttg gtcaagctgt atcgcaactt catcaggccc
     2161 ttgataaggc agcagataaa gtagccaaga agacacctgc tcagcaagat ttgatagggc
     2221 aagtggatgc cttgattgac acgctttact tcacctacgg cagttttgtc ttgatggggg
     2281 tggacccaga acgtattttt gatattgtcc atcaggccaa tatggggaaa atttttccag
     2341 atggcaaggc tcattttgat ccagtgaccc ataaaatctt aaaaccagat aattgggaag
     2401 aaaaatatgc tccagaacct gctattaaaa aggaactgca gcgccagctc aaggcttacg
     2461 aacgccataa agagagaaat aaatcgtaaa caaaaaggag ctaggggctc cttttctttg
     2521 tgattataag gttttttctt gttctctcac aaccagcaaa tcgaccttag catggcggag
     2581 tatgtattca gatgaagagc cgaccaagag gcgttcaaag gcgttgagac cagttgcgcc
     2641 aacgaggatg aggtccactt cctcggcatc tggaatagta cgtgctagca gggtctttgg
     2701 atttcccatt tcaatgacga tatgaacatc tgctacccca gcatctttag cacgtttttc
     2761 gtactctttc atcagacttt cagcgtcgac ttggagttct tcgtaaactt cagcatcaaa
     2821 ggtggatacg ctttggagag cgcgtgtgtc aatgacatgt gcgatggtga gtttagcgtc
     2881 gtttcgtaga gcagaatgaa ctcccttgac aaaagccaag tccgcttcct tagaaccatc
     2941 gattgcgacc atgatatttt ggtaacgttg tgccataatg aaacctcctg aaattttctt
     3001 actataattg taaacctttt cactatagaa gtaaagcaaa aaaagtattt ttcaaaagaa
     3061 tattagggct taaactctat ataaatatat gataaaataa aagaaaggat agcaggaaag
     3121 aagtgaagga agggagaatg atgaaaaact cttttcaaaa atcaaatttc ctttattatg
     3181 gaattatcct agtttcagtc ttggtagaag tgatcatgat ctgtcaatta gagagtctgg
     3241 taccgcttct ttatccaagt tttattggat ttttagtttt tcatgtactc taccatctaa
     3301 tcttatttct agttgcaaaa cgaagtgggc gttgggatta cttgatgata tgggggcttt
     3361 tcctcatgtt taatcttctt tatgactcct ttctaggctt gcttttttct aggtttatct
     3421 tttggtatgt gatgtgtctt tgcaaaatac ctgctatctc tacgttctta acctcgcctt
     3481 tagcaaggtt tttcttctat acgaaactag taacacattt cagtaaactt gtcgcattct
     3541 ctttctagtg gtataatgtt actatcctga tgaattgagg aaacaagatg aaagaatata
     3601 acaagtctag taagttagag catgttgctt atgatatccg tggtcctgta ttggaagaag
     3661 ccatgcggat gcgagcgaat ggagaaaaga ttttacgtct gaatacagga aatccagcag
     3721 aatttggctt tacagcgcca gacgaggtca ttcatgactt gattatgaat gcgcgtgata
     3781 gtgaaggcta ttctgactcc aaagggattt tctcagcccg taaggccatc atgcagtatt
     3841 gccagctgaa gaaattccca aacgtagata ttgatgatat ttaccttgga aatggtgtca
     3901 gtgagttgat tgtcatgtcc atgcaggggc ttttggacaa tggggatgag gtcttagtgc
     3961 caatgccaga ctatcctcta tggacggcgg ctgtcagcct agctggagga aatgccgttc
     4021 actatatctg tgatgaagct gtggaatggt acccagatat tgacgatatc aagtcaaaaa
     4081 tcacttccaa taccaaggca attgtcctta tcaatccaaa taacccaact ggagcccttt
     4141 atcctaagga actcttgctg gagattattg agattgcccg tcaaaacgat ttgattatct
     4201 ttgcggatga aatctatgac cgcatggtaa tggacggaca tgtgcatacg cctgtggcga
     4261 gcttggcacc agatgtcttc tgtgtcagca tgaatggtct gtcaaaatcc caccgcatag
     4321 caggtttccg tgtgggttgg atggtcttgt ctggccctaa gactcatgtt aagggctata
     4381 tcgaagggct caatatgctg tccaatatgc gcctttgctc taacgttttg gcccaacaag
     4441 tcgtacaaac ttccttgggg ggtcaccaat cagtcgatga attgcttctt cctggtggac
     4501 gaatctacga gcaaagaaat ttcatctata atgccattca agatattcca ggtttgtctg
     4561 ccgttaaacc caaggcgggg ctctatatct tcccaaaaat cgaccgcaat atgtaccgta
     4621 tcgatgatga tgagcagttt gtccttgatt tcttgaagca ggaaaaggtt ctcttggttc
     4681 atggtcgagg ctttaactgg caggaaccag accacttccg tatcgtttac cttcctcgtg
     4741 ttgatgagtt agcccaaatc caagaaaaga tgactcgttt cttgaaacag tatcgtagat
     4801 agggcttgca ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga
     4861 atcagataga atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat
     4921 acaaaatcta tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc
     4981 gtgcgcgcaa acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg
     5041 cagctcgtcg tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa
     5101 aaatcagtag gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca
     5161 aatggattcg gaagcgatgt tacagtagat tgaaactaga atagtacacc tctgtttcta
     5221 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tattttattt
     5281 tactataaag ttgaagtagg tggagatggt acagcaacaa tcgtctttaa agatggttca
     5341 gctattacaa ttccaggaaa tcaattggta gcacaagatc caaaagcaca agatagcact
     5401 aaactgactg ctgaaaaatc aactgttaaa gcacctgctc aaagagtaga tgtaaaagat
     5461 ataactcatt taacagatga agaaaaagtt aaggttgcta ttttacaagc aaatggttca
     5521 gcattagacg gagcgacaat caatgtagct ggagatggta cagcaacaat cacattccca
     5581 gatggttcag tagtgacgat tctaggaaaa gatacagttc aacaatctgc gaaaggtgaa
     5641 tctgtaactc aagaagctac accagagtat aagctagaaa atacaccagg tggagataag
     5701 ggaggcaata ctggaagctc agatgctaat gcgaatgaag gcggtggtag ccaggcgggt
     5761 ggatcagctc acacaggttc acaaaactca gctcaatcac aagcttctaa gcaattagct
     5821 actgaaaaag aatcagctaa aaatgccatt gaaaaagcag ccaaggacaa gcaggatgaa
     5881 atcaaaggcg caccgctttc tgataaagaa aaagcagaac ttttagcaag agtggaagca
     5941 gaaaaacaag cagctctcaa agagattgaa aatgcgaaaa ctatggaaga tgtgaaggaa
     6001 gcagaaacga ttggagtgca agccattgcc atggttacag ttcctaagag accagtggct
     6061 cctaatgctg ctcctaagac aacaagtgca ccgcaagcaa ctgcaggaac aatgcaagat
     6121 gttacctacc agtcacctgc tggcaaacaa ttacctaaca caggttcagc atcaagtgca
     6181 gcacttgcta gtcttggtct agtggtggca acaagtggtt ttgctttgct aggaagaaag
     6241 actagacgta gaaaatagaa cagctagaaa attctattct ctacttaaag ttagattata
     6301 agggggattt tgagaagtca tcaatcctag tgatgggtga gaaaagtgag aacccaagat
     6361 aatcacatac tttagctgaa taggaatatt ctatcaatgt agccaatctc ttctgtctct
     6421 aactgtggaa taggagatgg gcaatatcgg atagaaaaga tagcagaata gctctctatt
     6481 gaagagagga ggggaaaccg aaaaattagg tgcccctcct cttttttggt ataatagaag
     6541 atagaaaacg aggttagaag agatgatttt tgatacacat acacacttga atgtagaaga
     6601 atttgcaggt cgtgaggcag aagaaattgc cttggctgct gagatgggtg tgacacagat
     6661 gaatattgtt ggttttgata aaccgacgat tgagcatgcc ttggagttgg tagatgagta
     6721 tgagcagctc tatgcgacta ttggttggca tcctacagaa gctggtactt atacagagga
     6781 agttgaggct tacttgttgg ataagttaaa acattccaag gttgtggctt taggtgaaat
     6841 tggcttagat taccattgga tgacagcgcc caaagaggtg caggagcagg tttttcgccg
     6901 tcagattcag ctatctaagg acttggattt gccttttgtt gtccataccc gtgatgcgct
     6961 ggaagatacc tatgagatta tcaagagtga gggcgttggt cctcgtggtg gtatcatgca
     7021 ttcattttca gggacgcttg agtgggcaga gaagtttgtg gatcttggta tgaccatttc
     7081 cttctcagga gtggtgactt ttaagaaggc aactgacctc caagaagcag ctaaagagtt
     7141 acctttggac aagatgttgg tggaaacaga tgcgccttac ttagcacctg tacccaagcg
     7201 tggtcgtgaa aataaaacag cctatactcg ctatgtggtc gactttatcg ctgacttgcg
     7261 tggtatgacg acagaagagc tggcggtagc aacgactgca aatgcagaac gaatttttgg
     7321 actggacagc aagtaatgaa agagaaaatt tctcaagtta tcgtggttga agggcgtgat
     7381 gatacggtca atctcaaacg ttatttcgat gtggagacct atgagactcg aggttctgcc
     7441 atcaatgctc aggatataga gcggattcag cgcctgcacc aacgtcatgg agtcattgtc
     7501 tttacagacc cagattttaa tggggaacgg attcggcgca tgatcatgat ggtcattcca
     7561 acagttcagc atgcctttct caagcgagat gaagctgttc ccaagtccaa gaccaagggg
     7621 cgttctctgg gaattgagca tgccagctat gaagacctga aaacggctct agctcaggtg
     7681 acagaacaat ttgaacatga gagtcagttt gacattagtc gtagcgattt gattcgcctt
     7741 ggttttctag caggggcaga cagccgtaag cgtagagaat atctcggaga gactctccga
     7801 atcggctatt ccaacggcaa gcaactcctc aaacgcctag agttgtttgg ggttactttg
     7861 gcagaagtgg aagaagctat gaaatcttat gagtaggaaa gatgtagccg ttacaatttt
     7921 ttaagtttca cagtattttt cgaagcaggt agaagaggag gcgtctgatg ttaattggtc
     7981 aaaaaattaa agagattcgg atagaaaaag gaattagtcg tccagatttt tgtggagatg
     8041 agcaagaact gacagttcgt caactgtcgc gaattgaaag tggagcttcg caaccgagtt
     8101 tgcccaagtt agactatatt gctcgccggc taggagttcc agtttatagc cttatgccgg
     8161 atttttcagc tcttccttct gcttatttag aattgaaata ccagatttta cgtgaaccaa
     8221 tctatggtaa agaagaggag tacgataaga aggaagcgtg tttggaagag atttataaaa
     8281 catactttga taatcttcct aaagaagaac aattagcatg tgaagtattg caggcgtgtt
     8341 tggatacttc tagaactaga aggcctgaat atgcagagtt aatacttgag gaacatatgc
     8401 ctcagattat agaaaaagaa gcttattcaa taaatgatat gttgttgatt cgtttgtttt
     8461 tttatcaaat gctcattaga aaagatcttg ccaaatttat aaatcaaatc gaaaagctaa
     8521 tgctctttct tttggaacag aagaaggtaa ctcaaataga gaattacttt ataattagag
     8581 atactcttat ttcaggaatg tgttgtcttg aaaaggtagg agtaactgat tgttttaatg
     8641 attatctatc gtgtttacaa gaaattatgg ataaaactca agattatcaa aagaaacctc
     8701 ttgtatttat gtttttgtgg aagcaagcat taagagaaga aagagatttt agtttagctg
     8761 aatcatttta tcagtcttct aaaacatttg cgcagctaat tggagatgaa tttctagtaa
     8821 agaaattgac agaggaatgg caagaggatg tcaaaaaata tttataaaca tagtgaatca
     8881 gtgacaaaaa tgtccttgtc ctcgtatcaa aacagttcta aagttcgtct ttagggatgt
     8941 ttttttagat ataagctaaa aatgacacga aatggttaga ttttaaggac attgatgtcc
     9001 ttgatagttc ttgaaaaaac ggatataatg tatttgaaga tgttgctagc agaaagaact
     9061 taataagctt cttaacatcg cagtgactgt ggtcaccttc tcacgaagtg accaagtaac
     9121 tttttggagg aaatgatgaa acgtttattt attttgattt caatggtatt agtatcgctt
     9181 tatatggtga taacttccgt tgaccatcga gaagagattt tatttggtaa ctatccttct
     9241 gttgatgtga caggaatgat gataaatcaa ccagtagcta gtcgcgaaga ggtgacagag
     9301 gctttgagtc acttggcggt agagcacaat agtctcattg ctcgtcgaat cgttgagcca
     9361 aatgaagctg gagaaacacg ctttacctat gccacttatg gtgagggaaa gcttccagaa
     9421 ggtctgacca tttcctccaa ggagagtgca gaaacgagtg atttattagg gtcttacttg
     9481 attgtatcag gaagtttgga tggagtgagc ttacagacca ccttgaaaga gcttggttat
     9541 caaggctttg tttcgaatgg agaagatcca ttttcgatag tcttactatt gacggccacc
     9601 cctatggtgc tactgagttt agctattttt ctgctgacct ttatgagtct gaccctgatt
     9661 tatcggatca aatcccttcg tcaggcaggg attcgcttaa tagctggtga gagcttgttt
     9721 ggagttgctc tcagaccagt gttagaagat gtgagacagc ttatctgctc agtgctggta
     9781 tccagtcttt tgggattggg gattctctgg tatcaaggtg ccttgtttat ggcaacggtg
     9841 caactggtca tcattgctct tctactttat ggattgacct tggcagggat ttctacctta
     9901 ctaagtgtcg tctatctact tggtttacag gaaaatagtc tggtggatct attgaaaggg
     9961 aaactccctc tcaaacgtat gatgacattg atgatggtgg ggcaactctt agctgtattg
    10021 gtggtcggat cgagtgcgac agctctccta ccccactacc gtgaaatgca ggaaatggag
    10081 agagctagca ataaatggag ccagtcctca gaccgttacc gtctatcctt tggttggtct
    10141 agtgcatttg ccgatgaaga aggaacgcgt aaggataatc gtgagtggca gacatttact
    10201 gaagaacggt tagccaatac agactctttt tatattatga gcaatgttga caatttctca
    10261 gatggagcag aagtggacct agatggcaat cgtctcagtg actacacacc gtcagggaat
    10321 gttatctatg tctcaccgcg ctatctgata gaagaaaaga ttaccgtttc ttcagagttt
    10381 atggacaaga tgcaaaactt gtctgaggga gagtttgggc tgatcttgcc tgagagcttg
    10441 cgagagcagt ctgtctacta ccaaggattg tttacagatt acctgcaaaa cttttcatct
    10501 gaaagtgtag aagtgacgag tcagaaacac tacctcccac aggtaaggct agcttttaca
    10561 gaaacaggac aggaacgttt cctctataat gatgggtaca agacaacacg ccagtaccta
    10621 aaagatccga ttattgtagt tctaacgccg caagcgactg gaacaagacc tgttgcaggg
    10681 atgttgtggg gaactacggc taatagtgcc ttgaaactag atcgatatgg agacagcatc
    10741 acagctctaa aagagaaagg tctgtatcac aaggtttctt acttggtaaa aagccagcta
    10801 ttttttgcca aggtactaaa tgacaaacgg gtggagtttt actctctcct tattgggacg
    10861 attttgaccc tgtctacggc tatcttgtta tttgattcca tgaatcttct ctattttgag
    10921 cagttcagac gggaacttat gattaaacgt cttgctggta tgacaatcta tgagcttcat
    10981 ggcaagtatt tactggcgca aggaggagtt ctcttgcttg gcctagtcct atctagtatt
    11041 ttgacaagag atggtttgat tagcgctcta gttgtagctt tgtttacgct taacgccctc
    11101 ttgattttag taaggcagga caaaaaagaa gaagctggta gcatggcagt attgaaagga
    11161 aaataagatg attgatattc aaggattgga aaagaaattt aatgaccgcg cgattttctc
    11221 tggtttgaat ctcaagctgg agaagggcaa ggtttatgcc ttaatcggaa agagtggaag
    11281 cggaaagacg acgctgctga atatcttggg aaagctagaa aagatagatg gtggaagggt
    11341 tctctatcag gggaaagatt taaaaaccat tcccactcgt gagtattttc gagaccagat
    11401 gggctatctc tttcaaaatt tcggcctctt agaaaaccaa tcaatcaaag aaaatttgga
    11461 tttgggtttt gttggtcaga aaatctcaaa agtagaacgt ttggaaaggc aagtgggggc
    11521 tttagaaaaa gttaatctag ggtatttgga tttagaacaa aaaatctata ctttatctgg
    11581 gggagaggcc caacgagttg cccttgctaa gactattttg aaaaatccac ccttgatttt
    11641 ggcagatgaa ccaacagcag ctcttgatcc tgaaaattca gaggaggtta tgaatctctt
    11701 ggtggatttg aaagatgaaa atcgaattat catcattgcg acccataatc ccctagtctg
    11761 gaataaggct gatgaaatca ttgatatgag gaaacttgct catgtgtgaa aaaatccgta
    11821 ttcgcagggt atctgattat cctagtgcca gaggtggttt agaagatatc ctcatcatgg
    11881 aaaatatgac caatcatctc cttttggttc aaatccgagt gcatggctat ttgcttgatt
    11941 ttgctagtat tgaagggcaa aggcaaaagc attatcgttt gaaaaattta cctcagacgg
    12001 ttgaactgac agtggatgat gtggaggagg atgtggattt gaccctacct gaaaatcgaa
    12061 gttatcaaga agctgatttt tttgaacgca tgtttcgaga gaactgctaa ggccactttt
    12121 aaagatttcc aagactatct ttcttcatga ggaaagatag ttttttggta tgattttcat
    12181 tcccaaaata caaggggaat gtgttacaat agtagtaaca gataatagaa aagagaatag
    12241 atgagaattg cagattatag cgtgaccaag gcagtgctgg agcgtcacgg ttttaccttt
    12301 aaaaagtcct ttgggcaaaa ttttttgacg gataccaata tccttcaaaa aattgtggat
    12361 acggctgaaa ttgatgatca ggtcaatgtc atcgaaatcg ggccaggtat tggtgccttg
    12421 acagaatttt tggctgagcg tgcagcccaa gtcatggctt ttgagattga ccaccgtttg
    12481 gtgccaattt tggcagatac cctgcgtgat tttgataatg tgaccgtagt taacgaagat
    12541 attctcaagg ttgatttggc gcaacatatc cagaatttta aaaatcctga cctgccaatc
    12601 aaggtagtgg ctaatttgcc ttactacatc acgacgccta ttctcatgca cttgattgag
    12661 agtggcattc ctttttgtga gtttgtggtc atgatgcaga aagaagtagc ggaccgcatt
    12721 tcagcccagc ctaacaccaa ggcttacggt agcttgtcta tcgccgtgca gtattacatg
    12781 acagccaagg ttgcctttat cgtgcctcgt acggtctttg tgccagcgcc aaatgtggat
    12841 tcagccatct tgaaaatggt gcgtcgtcca gagccagccg tagcagtaga agatgagaac
    12901 tttttcttta aggtttccaa ggctagtttt acccatcgcc gcaagacctt gtggaataac
    12961 ttgacaggtt actttggtaa gactgaagag gtcaaggaca agctgaccaa ggctttggac
    13021 caggcaggct tgtcaccaag tgtgcgtggg gaagctctca gcttggcaga atttgccggt
    13081 ctagcagacg cacttaaagg gcaaggactc taagatgcag ggacaaatca ttaaagcctt
    13141 ggcaggtttc tactatgtgg agagtgatgg ccaggtttat caaacacgcg cgcgtgggaa
    13201 tttccgtaaa aaaggccata ccccttatgt tggggactgg gtagatttct ctgccgagga
    13261 aaattcagaa ggctatatcc tcaaaattca cgaacggaaa aacagtctgg ttcgtccgcc
    13321 tattgtcaat atcgatcaag ctgtagtaat catgtccgtc aaggaacctg attttaacag
    13381 caatttgctg gatcgtttct tggttctttt ggagcacaag ggcatccatc ccattgtcta
    13441 tatttccaaa atggatttgt tggaagatag gggagaactg gatttttacc agcagaccta
    13501 tggtgacatc ggctatgact ttgtgaccag taaagaggaa ctcctgtctt tgttaacagg
    13561 caaggttacg gtctttatgg ggcagacagg tgttgggaag tcaactcttc tcaataaaat
    13621 cgcaccagac ctcaatcttg aaacgggaga aatttcagac agtctaggtc gcggtcgcca
    13681 taccactcga gctgttagtt tttacaatct caacgggggt aaaatcgcag atacaccagg
    13741 attttcatcc ttggactatg aagtatcaag ggctgaagac ctcaatcagg ctttcccaga
    13801 gattgctact gttagccgag attgtaagtt ccgtacttgt acccataccc atgagccgtc
    13861 ttgtgccgtc aaaccagctg ttgaagaggg tgttattgca accttccgtt ttgacaatta
    13921 cctgcaattc cttagtgaaa ttgaaaatcg tagagaaacc tataaaaaag tcagcaaaaa
    13981 aattccaaaa taaggagaaa cctatgtctc aatacaagat tgctccgtca attctggcag
    14041 cagattatgc caactttgaa cgtgaaatca aacgtctaga agcaactggg gcagaatatg
    14101 cccatatcga tatcatggac agtcattttg taccgcaaat cagttttggt gcaggtgtgg
    14161 tcgagagcct tcgtcctcat agtaagatgg ttttcgattg ccacttgatg gtgtcaaacc
    14221 ctgagcatca tctggaagat tttgcgcgtg caggtgcaga catcatcagt atccatgtag
    14281 aagcaacgcc tcatattcat ggcgccctcc aaaaaattcg ttcactcgga gttaagcctt
    14341 cagtcgttat caatcctggc acatcagttg aagccatcaa gcacgtcctt catctagttg
    14401 accaagtttt agtcatgacg gttaatccag gttttggtgg gcaagccttt ctgccagaaa
    14461 ccatggataa ggtccgtgag ttggttgctc ttcgtgagga aaaaggtttg aactttgaaa
    14521 tcgaagtgga tggtgggatt gatgaccaaa ctattgctca agccaaagaa gccggtgcga
    14581 ctgtttttgt agcaggttcc tatgtcttta agggagaagt caatgagcga gtacaaactc
    14641 tcagaaaaca actggactag ggttgcagtt tttgcaggcg gaaaccgcgg tcattatcgg
    14701 acagattttg atgcttttgt tggggtggat cgaggctcgc tctgggtctt ggaagaagac
    14761 ttacctcttg ctctagcagt cggagatttt gattctgtga cggaagaaga gcgacaggtg
    14821 attcaaaaag gtgcccagta ttttgtccaa gcacgaccag aaaaggatga tacagatctg
    14881 gaattggctc tcttaaccat ctttgaacaa aatcctcagg ctcaggtcac tattttcggt
    14941 gccttgggtg gccgtattga ccatatgttg gccaatgtct ttctgcctag caatcctaag
    15001 ttggcaccct atatgcatca aatagaaatt gaggatgggc aaaacttgat tacttattgt
    15061 ccagaaggaa tcagtcagct agaacctcgt tcagactacg actatctagc ctttatgcca
    15121 gttcgggata gccagctgac tattcttgga gccaagtatg agttgacaga ggaaaatttt
    15181 ttctttaaaa aagtgtacgc ttctaacgaa tatatagata gggaagtgtc ggtaacttgc
    15241 ccagatggtt atgtggtcgt actgcatagc aaggacagga ggtaggatgg aaagtttact
    15301 tattctatta ttaattgcca atctagctgg tctctttctg atttggcaaa ggcaggatag
    15361 gcaggagaaa cacttaagta agagcttgga ggatcaggca gatcatttgt cagaccagtt
    15421 ggattaccgc tttgaccaag ccagacaagc cagccagtta gaccaaaaag atttggaagt
    15481 ggttgtcagc gaccgtttgc aagaagtgcg gattgaattg caccaaggtc tgacccaagt
    15541 ccgtcaagaa atgacagata atctcctcca aactagagac aagacagacc aacgtctcca
    15601 agccttgcag gaatcaaatg agcaacgttt ggaacaaatg cgccagacgg tcgaggaaaa
    15661 actagaaaag accttgcaga cacgcttaca ggcttccttt gagacagttt ctaaacaact
    15721 ggagtctgtc aatcgtggcc ttggagaaat gcagacagtt gcccgtgatg tcggagctct
    15781 taacaaggtt ctctctggaa ccaagacgcg agggattctg ggagaattgc aactggggca
    15841 aattattgaa gacatcatga cacctgccca gtacgaacga gaatacgcaa cggttgaaaa
    15901 ctctagtgaa cgagtggagt atgccatcaa gttacccgga caaggcgacc aagaatacgt
    15961 ctatctgcca attgactcta agtttccact ggcagattat taccgcttgg aagaagccta
    16021 tgagacaggt gacaaggatg agattgaacg ctgtcgtaag tcactcctag caagcgtcaa
    16081 gcgctttgct agggatatta ggaacaagta catagcacca cctcggacga ccaattttgg
    16141 agttttgttt gttccgacag aaggtctcta ctcagaaatc gtccgcaatc cggtcttctt
    16201 tgatgatttg agacgggaag aacagattat tgttgcagga ccaagtaccc tatcagccct
    16261 tcttaactcc ctatcagttg gtttcaagac ccttaatatc caaaagagtg ccgaccatat
    16321 cagcaagact cttgccagtg tcaagaccga gtttggcaag tttggtggta ttctggtcaa
    16381 ggcacaaaaa catctccaac atgcctctgg caatattgat gaattattaa accgtcgtac
    16441 catagctatc gagcggacgc tccgtcacat tgagttgtca gaaggtgagc ctgcgcttga
    16501 tctactccat tttcaagaaa atgaggaaga atatgaagat tagtcacatg aaaaaagatg
    16561 agttatttga aggcttttac ctaatcaaat cagctgacct gaggcaaact cgagctggga
    16621 aaaactacct agcctttacc ttccaagatg atagtggcga gattgatggg aagctctggg
    16681 atgcccaacc tcataacatt gaggccttta ccgcaggtaa ggttgtccac atgaaaggac
    16741 gccgagaagt ttataacaat acccctcaag tcaatcaaat tactctccgc ctgcctcaag
    16801 ctggtgaacc caatgaccca gctgatttca aggtcaagtc accagttgat gtcaaggaaa
    16861 ttcgtgacta catgtcgcaa atgattttca aaattgaaaa tcctgtctgg caacggattg
    16921 tccgaaatct ctacaccaag tatgataagg aattctactc ctatccagct gccaagacca
    16981 accaccatgc ctttgaaacg ggcttggcct atcatacggc gaccatggtg cgtttggcag
    17041 acgctattag cgaagtttat cctcagctca ataagagcct gctctatgcg gggattatgt
    17101 tgcatgactt agctaaggtc atcgagttga cggggccaga ccagacagag tacacagtgc
    17161 gaggtaatct tcttggacat atcgctctca ttgatagcga aattaccaag acagttatgg
    17221 aactcggcat cgatgatacc aaggaagaag tcgttttgct tcgtcatgtc atcctcagtc
    17281 accacggctt gcttgagtat ggaagcccag tccgtccacg cattatggaa gcagagatta
    17341 tccatatgat tgacaatctg gatgcaagca tgatgatgat gtcaacagct cttgctttgg
    17401 tggataaagg agagatgacc aataaaatct tcgctatgga taatcgttcc ttctataaac
    17461 cagatttaga ttaataattt aagaaaaatg agcatttttt aggataagaa tgttcgtttt
    17521 tttatgtgaa tatggtataa taagtaaaag acaaaaatga atactcttcg aaaatctctt
    17581 caaactaggg tagtatcgcc ttgtcgtatg tatatatgca ggtatattac agggtttgtc
    17641 agttctattg acaatctcaa aacagtgttt tgaaccacca gcgaccagct ttctagtttg
    17701 ctttttgatt ttttgaataa aaatggaata ggaaatagaa atgaaattaa gaagaagtga
    17761 tcggatggtt gtcatttcca actatttgat taataatcct tataaactaa ctagtctcaa
    17821 tacttttgct gaaaagtatg agtctgctaa atcatccatc tcagaagata tcgtcattat
    17881 caaacgcgcc tttgaggaaa ttgaaatcgg tcatatccag acagtgactg gggctggcgg
    17941 aggtgtcatc ttcacaccgt ctatttcgag tcaggatgct aaggaaatgg ttgaagactt
    18001 gcgtaccaag ttgtcagaaa gtgaccgtat cttgccaggt ggttatatct atctgtctga
    18061 tttgcttagc acaccagcca tcttgaaaaa tattggtcgt attattgcca aaagctttat
    18121 ggaccaaaaa attgacgcgg ttatgaccgt agcaactaag ggtgtgccac ttgcaaatgc
    18181 agttgccaat gtcctcaatg tctcttttgt cattgtgcgc cgtgacctga aaattaccga
    18241 aggttcaact gttagcgtca actatgtttc aggttcaagt ggtgaccgta tcgagaaaat
    18301 gttcctttca aaacgtagtc ttaaggcagg cagccgtgtc ttgattgtgg atgacttctt
    18361 gaaaggtggc ggaacggtca atggtatgat tagtctcttg cgcgagttcg actcagaact
    18421 ggcaggtgta gcggtctttg cggacaatgc ccaagaagaa cgtgaaaagc agtttgacta
    18481 caagtcactc ttgaaggtaa ccaatattga tgtcaagaac caagccatcg atgttgaggt
    18541 tggcaatatc tttgacgaag ataaataaga gatagaacta aaggttggaa cgattgtccc
    18601 agcctttctt tgcaaacaga atagaaggaa gcttatgaaa acaccattta tcaatagaga
    18661 agagttagaa gcgattgttg ccgagttccc gactcccttt cacttgtatg atgagaaggg
    18721 gattcgtgag aaggcaagag ccgtcaacca agctttttcg tggaacaagg gctttaagga
    18781 atattttgca gttaaggcta ctccaactcc agctattttg aaaattctcc aagaagaagg
    18841 ttgtggtgtg gactgctcta gttatgtaga gcttttgatg agccataaac tggactttct
    18901 gggttctgag attatgttct cttccaacaa cacgccagac aaggaatacg cctatgcacg
    18961 tgaattgggt gcgaccatta acttggatgc ctttgaagat attgaacatc tggagagagt
    19021 agcaggcatt ccagaaatca tctcttgtcg ttataatcct ggaggcgttt ttgaactggg
    19081 gacagacatt atggacaatc ctggggaggc taagtttggc atgaccaagg accagctctt
    19141 tgaagccttt gctatcttga aggaaaaagg agccaagact tttgggattc actccttcct
    19201 agcgtccaat accgtgaccc atctctatta tccagagttg gctcgtcagc tctttgaact
    19261 ggctgttgaa atcaaggaaa agttgggcat ttcgctagac tttatcaatc tttctggcgg
    19321 tattggtgtt aattatcatc cagaccagga gccgaacgat atcgccttga ttggtgaggg
    19381 agttcgtaag gtgtatgaag aggttcttac gtcagcaggt cttggtcagg tcaagatttt
    19441 caccgaattg ggtcgtttta tgctggcacc tcacggtgct ctagtcacaa gagtcactca
    19501 taagaaggaa acctaccgta cctatctagg tgtggatgcc tcagcagtca acctcatgcg
    19561 tccagctatg tacggagctt accatcatat tagcaacgtg acccatccag atggaccagc
    19621 tgaagtggta gatgtggtcg gttcgctctg tgaaaacaat gataaatttg cagttaatcg
    19681 cgaactgcct catacagaaa tcggtgattt gctggtcatt catgatacag gtgcccacgg
    19741 attttcaatg ggctaccagt ataatgccaa attacgttct gcggaaatcc tctataccga
    19801 agaaggtaaa gcccgtcaaa tccgccgtgc agagcgccct gaggactatt ttgcaacctt
    19861 atatggcttc gattttgaag aataatctga taatagattg aaaatgaaat tgaaaaacag
    19921 attgctttct aaaaaatagg caaaaatctt gtttttcctt caagtcgtga tataataaaa
    19981 ctataaaacg ttttcaagga aggtaacgat atgtctgaag aaacaattga ttatggacaa
    20041 gtgacaggaa tggtgcattc gacagaaagc tttgggtcag tagatgggcc tggtattcgc
    20101 tttattgtct ttttgcaggg ctgtcacatg cgttgccagt attgccacaa cccagacact
    20161 tgggctatgg agtccaataa gtcacgtgaa cggacggtag atgatgtctt gacagaggcc
    20221 ttgcgctacc gtggtttctg gggaaataag ggtgggatta cagtcagtgg aggagaagct
    20281 ctcttgcaga ttgatttcct gattgctctc ttcaccaagg ctaaggaaca aggaatccac
    20341 tgtaccttgg acacctgtgc tcttcctttc cgtaataaac cacgttacct tgagaagttt
    20401 gacaaactca tggctgtcac tgacttggtt cttttggata tcaaggaaat caacgaagaa
    20461 cagcacaaga ttgtcactag ccaaaccaat aaaaatatct tggcttgtgc ccagtatcta
    20521 tcagatattg gaaaacctgt ctggattcgc cacgtgctag ttccaggatt gacagacaga
    20581 gatgatgact tgattgaact tggtaagttc gtcaagaccc tcaaaaatgt tgataagttt
    20641 gaaattctac cttatcacac catgggtgag ttcaagtggc gtgaacttgg aattccatat
    20701 tccctcgaag gagtcaaacc accaacagca gatcgcgtca agaacgctaa acaactcatg
    20761 gataccgaaa gttatcaaga ttatatgaaa cgtgtacatg gatagaaaag aagcctgatg
    20821 gaaacatcgg gcttttgact tgcaaaaaga cttagcaaat cagctaagcc tttttcttct
    20881 tatctcgaac gttgttttcc agcgttgcga tttttgtgtt ttttcttgct tgtgatagca
    20941 gttggttgtt caggggtaac gtcttttcgt ccacttggtt tagagaaagc acttgctttt
    21001 ggtgggttct tggctagttc ttcacggact tttttgcgaa gttttggacg aacgatatag
    21061 ttgacgataa actgttggag aatcatcatg aaaccaccga caacccagta aagtgtgaca
    21121 ctagctggtg agaagaggga gaagacgacg atcatgagtg ggctcatgta aatcattttc
    21181 ttgatttgtt ctctttgcat ttcatcttct actccgtgaa gtgaaaggag cgattgaaga
    21241 tagtaaagga caccagcaca ggcaaccaaa atcatacttg gagaacctag aggaatgcct
    21301 aggtagcttg cttgagcaac cccttcagta tgttgggcag caaagtagat agcagagaag
    21361 aaaggcattt gaaggaggat agggaaacat cctacaccgc caaacatgct gataccgtgc
    21421 tctttttgag cagcaaagag agcttgttgg gcttcgagtt tttcttcttg agtagtcgct
    21481 tctttgagac gcgtttggtg tggctcaagg acgtgcttga gggcgttcat cttttcagag
    21541 tgaagcgttg ccttccatga ttggtagata ccaagtggta agataatcaa gcgtacgata
    21601 atggttacga taatgatagc gacaccaaag cctagacctt tatcagtagc gaagtacttg
    21661 atggcttcag ccataggcgc tccgatcgta ttccaaataa atcctgttgg ctgacctgtg
    21721 gttttatcga cattgacaca gccagtcaag acaagcaaca tagccactcc catagccgag
    21781 agtgcaaaac gtttaataga tttcactgtt tttattcctt ctttaaaaat tatacctttc
    21841 tattctactg tttttttaca aaatatacaa tagttctgga gacctaattt gcgattttaa
    21901 agtcagggta gggaggaaag ttgctcagtt tgacatctaa gtaactgact tttgaaaaag
    21961 gtgtcggtcc ttttcggatt tcttggataa attttgccat aatagctgat gagtctgctt
    22021 gggctaagat ttccactgtg ccatcgtcgt tattccatac ccgacctgtg atgccaccga
    22081 tttcaagggc caggctataa acaccccaac gaaagccgac tccctgcacc ctaccttggg
    22141 caatcattct aaccttttgc ataccaaacc cctttgattg tgttataata tttctatgac
    22201 tattataacc tcaaaagcca attctgtggt aaaaaatacc aagaaattac accaaaaaaa
    22261 ataccgcaag tcagcctatt tgattgaagg ctggcacttg tttgaagaag ctgttcaagc
    22321 tggagtgacg attgagaaaa tctttaccct agaaaattat cgagatcagt tagcaacttt
    22381 ttcgcaaact gtctgggtgt cagaggatat tttgctagat ttggcggatt ctcagactcc
    22441 acagggaatt gtggccgtgg ttcaaaaaga agaagtagga caagctgatt tgagtcaggg
    22501 caagttcttg tttttggaag atgtgcaaga tcctggtaat gtaggaacta tcattcgaac
    22561 tgcggatgca gcaggtttta caggagtgat tgtttcagat aagtcggcag acatctacag
    22621 tctcaaaacc ctacgttcca tgcaaggtag tcatttccat ctgcccattt accggatgac
    22681 tagtcaagcg cttcttgacg aaactaaaaa ggtagctatc ccagtgttag caacaaccct
    22741 atctaaagat tctgttgatt acagagaact gccttctata gaaaattttg tactagttat
    22801 gggaaatgag ggtcaaggaa ttagtcccct tatggctgaa agtgcagacc agttggtcca
    22861 tattagcatg aaggggcagg ccgagagttt gaatgttgcg gttgcagccg gtattttaat
    22921 cttccattta agctaatttt aacttttttt gttataatca aggaaagatg ttcacagaaa
    22981 aggagaaatg gatgaatcac actattatac atgaccgtgc aggtctcaat caattttacg
    23041 ctaaggttta tgcctttgtt ggtctgggaa tcggactatc tgctttggta tcaggcctta
    23101 tgttgacggt ctttcagtct cagttggttt acttgttgat gcaggggcgt ctctggttga
    23161 ccattgctac ttttgcggag ttggctttgg ttttcgttgc cagtagcatg gcttcaagaa
    23221 atagtccagc tgctcttcca gtatttttac tttactccgt tttaaacggc tttaccctta
    23281 gttttgtggt ggctttctat actccgggta cagttttatc tgcctttgta tcgagcgccc
    23341 ttctcttctt tgtcatggcg gcagttggta tctttaccaa gaaagacttg agtgggattg
    23401 gtcgagcaat gatggcagct ctcattggtt tactgattgc tatggtagtc aatattttct
    23461 tggctagtgg cttctttgat tatatgatta gcgtagccat ggttttggtt ttttcagggt
    23521 tgattgcttg ggataaccaa aagattcgcc tcgcctatga acaatcacaa ggtcgtgtag
    23581 cgacaggctg ggtggtgtca atggctctca gcatctatct tgattttatc aatcttttcc
    23641 taagtattct acgtatcttt ggtcgcaatg actaaaaggt ctatgaaatc agtgttttga
    23701 gtgacactga ttttttttaa attttctctt ttcttttctt ggaaataggt gtataatacg
    23761 tttattaaat ttttgaggag ttgtctatga agaaaagttt tatccatcaa caagaagaaa
    23821 tttcctttgt caaaaacact tttacccagt atttgaaaga taagctagaa gttgtcgaag
    23881 ttcaaggtcc tatcttgagt aaggtcggtg acggaatgca ggacaacctg tctggtgtgg
    23941 aaaatccagt atcggtcaag gttctccaaa tccctgatgc tacttatgaa gtggtgcact
    24001 cacttgctaa atggaaacgc cacaccttgg ctcgttttgg ctttggtgaa ggagagggtc
    24061 tctttgtcca catgaaagcc cttcgtccag atgaggattc cttggatgca acccactctg
    24121 tttatgttga ccagtgggac tgggagaagg ttatcccaaa tggtaagcgt aacatcgttt
    24181 atctaaaaga aacagttgag aagatttata aggctattcg cctgactgag ctagctgttg
    24241 aagcccgcta tgacatcgag tctatcttgc caaaacaaat tacctttatc catacagaag
    24301 aattggtaga acgctaccca gacttgacac cgaaagaacg tgaaaatgcg atttgtaaag
    24361 aatttggagc cgtctttttg attggtatcg gtggcgagtt gccagatggt aaaccgcacg
    24421 atggacgtgc accagactat gatgactgga caagcgagtc tgagaatggc tacaagggtc
    24481 taaatggtga tattcttgtc tggaatgagt ctttaggtgg agcctttgag ttgtcttcta
    24541 tgggaattcg tgtagatgaa gaaactctta gacgtcaggt ggaaattaca ggtgatgaag
    24601 accgcttgga attggaatgg cacaagtctt tgttgaatgg tctattccca ttgacaatcg
    24661 gtggaggaat tggacaatct cgtatggcca tgttcctact tcgcaagaga cacatcggag
    24721 aagtgcaaac aagtgtttgg cctcaagaag tccgcgatac ttacgaaaat attttgtaga
    24781 gaatcgaacc gcaaggttcg gttttctttc tctttttgtc tataatttgg tataataaac
    24841 agtatgaaaa tcgtatcagg aatctatggg ggacgtcccc tcaagacact agaaggcaag
    24901 acgacaagac ctacttcgga taaggttagg ggagccattt ttaacatgat tggtccctac
    24961 tttgaagtgg gacgagtctt ggacctttat gcaggtagtg gtggtttatc tatcgaagca
    25021 gtatcgcgtg gcatgtccag tgctgttttg gtggagcgag accgtaaggc tcagaccatc
    25081 gtggctgaaa atatccagat gaccaaggaa gttggaaaat ttcaactcct caagatggat
    25141 gcagaaaggg cattggaaca ggtatctggg gaatttgacc tcgttttctt agaccctccc
    25201 tatgccaagg aacaaatcgt agcagatatt gaaaaaatgg ctgagagaga gcttttttct
    25261 gaagatgtta tggttgtgtg cgagacggat aaagccgttg aacttccaga agaaattgcc
    25321 tgtctgggta tctggaagga aaagatttat ggaattagta aggtgacagt ctatgtcaga
    25381 taagattggc ttattcacag gctcatttga tccgatgaca aatgggcatc tggatatcat
    25441 tgaacgggcg agcagacttt ttgataagct ttatgtgggt atttttttta atccccacaa
    25501 acaaggattt ctccctcttg aaaatcgtaa acgggggtta gaaaaggctg tgaaacattt
    25561 gggaaatgtt aaagtcgtgt cttctcatga taaattggtg gtcgatgtcg caaaaagact
    25621 gggggctact tgcctagtgc gaggtttgag aaatgcgtcg gatttgcaat atgaagccag
    25681 ttttgattac tacaatcatc agctgtcttc tgatatagag actatttatt tacatagtcg
    25741 acctgaacat ctctatatca gttcatcagg cgttagagag cttttgaagt ttggtcagga
    25801 tattgcctgc tatgttcccg agagtatttt ggaggaaata agaaatgaaa aaaaagatta
    25861 gatggccctt atacgtcatt gcggccttga ttgtgacttt cttggcattt gtagtgccct
    25921 tgccttatta tatagaggtt ccaggtggtt cggaagatat tcgccaagtc cttaaagtaa
    25981 atgacacaga agataaggaa gctggtgcct atcaattcgt tacggttggt gttcaacatg
    26041 ccactttagc tcatatgatt tatgcttggt tgacaccttt tacagatatt cgtagtgctc
    26101 aggagactac aggtggttct tccgatgttg aatttatgcg aatcaatcaa ttctacatgc
    26161 aaacatcgca aaatatggcc aagtatcaag gactaaaaac agctggtaag gatatcgaac
    26221 tcaagtactt tggagtttat gttttgaatg tgacggataa ttcaaccttt aaagggattc
    26281 tcaatatctc tgatacagtc acagcagtca atgatcagac ctttgatagt tccaaagact
    26341 tgattgatta cgtcagttct caaaaattag gggattccgt caaggtcacc tatgaagagg
    26401 atgggcaaac caagtctgca gaaggaaaaa tcatcacctt ggaaaatggc aaaaatggaa
    26461 ttggaatcgg cttgattgac cgtacagagg taatcagcaa tgtcccaatt agcttttcaa
    26521 cagctggtat tggcggtcca agtgctggtc tcatgtttag tctagctatc tatactcaaa
    26581 tagctcaccc agatcttcgt aatggtcgta ttgttgccgg tacaggtacc attgaccgcg
    26641 atggtaatgt gggagacatt ggaggtattg ataagaaggt tgtagcttcg gctagggcag
    26701 gtgctgctat tttctttgct cctgataacc ctgttagcga agaagaacaa aaggcgcatc
    26761 cggacgcgaa aaacaactac caaacagccc tagaagcggc taaaacaatc aagacggata
    26821 tgaaaatcgt gcccgttaaa accctacaag atgcgattga ttacttgaaa aacaatccct
    26881 aatttttgag tgaagtttcc aaactagcgc acaaacagag aagatggtat aatagtcaaa
    26941 tggttcaatt attattcact ctaagtagtc atatgttctt tatttatgtg agtttttacc
    27001 ttttaaagga tcttgttaga tgggagaagg ttttaaaagt gacagatgat aatacaagaa
    27061 aagttcgttt attagtagcc ttttttagca ttgtcatagg ctacatcctg agttctttct
    27121 ttattagcct gtatcatttg tggcaagaag cgcttagagg attattatga aatcaagagt
    27181 aaaggaaacg agtatggata aaattgtggt tcaaggtggc gataatcgtc tggtaggaag
    27241 cgtgacgatc gagggagcaa aaaatgcagt cttacccttg ttggcagcga ctattctagc
    27301 aagtgaagga aagaccgtct tgcagaatgt tccgattttg tcggatgtct ttattatgaa
    27361 tcaggtagtt ggtggtttga atgccaaggt tgactttgat gaggaagctc atcttgtcaa
    27421 ggtggatgct actggcgaca tcactgagga agccccttac aagtatgtca gcaagatgcg
    27481 cgcctccatc gttgtattag ggccaatcct tgcccgtgtg ggtcatgcca aggtatccat
    27541 gccaggtggt tgtacgattg gtagccgtcc tattgatctt catttgaaag gtctggaagc
    27601 tatgggggtt aagattagtc agacagctgg ttacatcgaa gccaaggcag aacgcttgca
    27661 tggtgctcat atctatatgg actttccaag tgttggtgca acgcagaact tgatgatggc
    27721 agcgactctg gctgatgggg tgacagtgat tgagaatgct gcgcgtgagc ctgagattgt
    27781 tgacttagcc attctcctta atgaaatggg agccaaggtc aaaggtgctg gtacagagac
    27841 tataaccatt actggtgttg agaaacttca tggtacgact cacaatgtag tccaagaccg
    27901 tatcgaagca ggaaccttta tggtagctgc tgccatgact ggtggtgatg tcttgattcg
    27961 agacgctgtc tgggagcaca accgtccctt gattgccaag ttacttgaaa tgggtgttga
    28021 agtaattgaa gaagacgaag gaattcgtgt tcgttctcaa ctagaaaatc taaaagctgt
    28081 tcatgtgaaa accttgcccc acccaggatt tccaacagat atgcaggctc aatttacagc
    28141 cttgatgaca gttgcaaaag gcgaatcaac catggtggag acagttttcg aaaatcgttt
    28201 ccaacaccta gaagagatgc gccgcatggg cttgcattct gagattatcc gtgatacagc
    28261 tcgtattgtt ggtggacagc ctttgcaggg agcagaagtt ctttcaactg accttcgtgc
    28321 cagtgcggcc ttgattttga caggtttggt agcacaggga gaaactgtgg tcggtaaatt
    28381 ggttcacttg gatagaggtt actacggttt ccatgagaag ttggcgcagc taggtgctaa
    28441 gattcagcgg attgaggcaa gtgatgaaga tgaataagaa atcaagctac gtagtcaagc
    28501 gtttactttt agtcatcata gtactgattt taggtactct ggctctagga atcggtttaa
    28561 tggtaggtta tggaatcttg ggcaagggtc aagatccatg ggctatcctg tctccagcaa
    28621 aatggcagga attgattcat aaatttacag gaaattaggc tggagaacca gcctttttct
    28681 aaagataagg agaaatatga acaaaaaaac aagacagaca ctaatcggac tgctagtgtt
    28741 attgcttttg tctacaggga gctattatat caagcagatg ccgtcggcac ctaatagtcc
    28801 caaaaccaat cttagtcaga aaaaacaagc gtctgaagct cctagtcaag cattggcaga
    28861 gagtgtctta acagacgcag tcaagagtca aataaagggg agtctggagt ggaatggctc
    28921 aggtgctttt atcgtcaatg gtaataaaac aaatctagat gccaaggttt caagtaagcc
    28981 ctacgctgac aataaaacaa agacagtggg caaggaaact gttccaaccg tagctaatgc
    29041 cctcttgtct aaggccactc gtcagtacaa gaatcgtaaa gaaactggga atggttcaac
    29101 ttcttggact cctccaggtt ggcatcaggt caagaatcta aagggctctt atacccatgc
    29161 agtcgataga ggtcatttgt taggctatgc cttaatcggt ggtttggatg gttttgatgc
    29221 ctcaacaagc aatcctaaaa acattgctgt tcagacagcc tgggcaaatc aggcacaagc
    29281 cgagtattcg actggtcaaa actactatga aagcaaggtg cgtaaagcct tggaccaaaa
    29341 caagcgtgtc cgttaccgtg taacccttta ctacgcttca aacgaggatt tagttccctc
    29401 agcttcacag attgaagcca agtcttcgga tggagaattg gaattcaatg ttctagttcc
    29461 caatgttcaa aagggacttc aactggatta ccgaactgga gaagtaactg taactcagta
    29521 aaagataagc ctaaactcct atgtcactta tggatgtagg agttcttttt actagtttaa
    29581 gcaggactag aacaggtact aagaaaaaat agcaacttct aaaactaact tccagttttg
    29641 ggagagagat ggaagttact ttgagaagtt acggtaagaa aaaataatat gtattggatt
    29701 aaattttgtg atataataaa ggataagata ctattttagg agaagaacta tggaagaccc
    29761 aagtagtcag aatttgttgc tacaatttgt tttattgttt atcttgacgg tattaaatgc
    29821 ctttttctca gccacagaaa tggccatggt ttctcttaat cgtgcccgag ttgaacaaaa
    29881 ggcggaagaa ggagacagac gctacatccg cctgctgaag gtactagaaa atcctaacca
    29941 ctttttatca accatccagg taggaatcac cctgattaca atcttatcag gggcgagttt
    30001 aactgatact ctaggacgtg tgattgcctc ttggcttggg aatggcgaaa cagcacaagc
    30061 cgtggcaact tttctatcct tggcattttt gacctatatt tccatcgttt ttggggaatt
    30121 atatcctaag agaatcgctc ttaatctaaa ggatgccttg gcaattcgta cagcgccgat
    30181 tatcattggg atcggaaaac tagtcagtcc ttttgtttgg cttttagctg cttcgaccaa
    30241 tttcttgagc catttgactc ctatgtcatt cgatgatgcg gatgaaaaga tgacccgtga
    30301 tgaaattgcc tatatgctga caaatagtga agaaacattg gatgctgatg agattgagat
    30361 gctacaaggt gtcttttcgc tcgatgaact gatggcacga gaggttatgg ttcctcgaac
    30421 ggatgccttt atggtggata ttcaggatga tagtcaagcc attatccaaa gtattttaaa
    30481 acaaaattat tctcgtatcc cggtttatga tggggataag gacaatgtaa ttggaatcat
    30541 tcacaccaag agtctcctta aggcaggctt tgtggacggt tttgacaata ttgtttggaa
    30601 gagaatttta caagatccac tttttgtacc tgaaactatt tttgtggatg acttgctaaa
    30661 agaactgcga aatacccaaa gacaaatggc aatcttgctg gatgaatacg gtggtatggc
    30721 tggtttggtg actttggaag acctcttaga ggaaattgtt ggtgaaattg atgatgaaac
    30781 ggacaaagca gcaatagatg ttcatcagat tggcgaggat acctacattg ttcaaggaac
    30841 tatgaccctc aatgatttca ataattactt tgatgttgaa ctggaaagtg atgacgttga
    30901 tactatcgct ggttattatt tgacaggagt gggaactatt ccaacgactg agaaactcag
    30961 ctatgaattg gtcagccaaa acaaacagtt tatcttaacc aatgataaag tgaagaatgg
    31021 acgtgttacc aaggtaaaag tccaaatcac agaagtcgaa atagaagaag aaacagaata
    31081 aactagaggc ttttgtcacc aggcgtgacg aagctttttt caattgattt caaaagggaa
    31141 ttatgccatg gaaagaactc tgccacaaac tcgctccaaa agtttttaaa gttatacgta
    31201 tttattcgag ggaaaataaa aaaagccctt caaattgggc tttctgttca tttgaaacct
    31261 gataaatcaa gttatagaag gcggtagacg gatttgaacc gacgatcaag cttttgcaga
    31321 gccgtgcctt accacttggc tataccgcct taacgtttat tattatacct tgaaaatgat
    31381 tttcagtcaa tagttttttc agttttttgt accatagcgt gattcgaatg tgatgccaag
    31441 ttttctatgg gaaaaaattt accaaaaacc tactgaaacc ccttgaaaaa tctagctaaa
    31501 tagggtataa tatgggtaat catttgtcgt aggttttgtc tgaaatattg tccagacaag
    31561 gctcacagca gttaaatctt ctgaaaaagt cagatttagc tgctcttttt gtgctttttt
    31621 tcaagatttt gagcatttgt aacagaggct taaagattct gaaaattcgt caagaggaca
    31681 cggtgataag gggtttacaa ccatatgacg attagaaaag cctgattgac aaggcttgga
    31741 acttatttac aaaggagaat catcttggca ggacatgacg ttcaatacgg gaaacatcgt
    31801 acccgtcgta gtttttcaag aatcaaagaa gttcttgact taccaaattt gattgaaatt
    31861 caaactgact cattcaaagc tttcctagac cacggtctta aggaagtgtt tgaagatgta
    31921 ttgccaattt caaacttcac agacacaatg gagttggaat ttgttggata tgaaatcaag
    31981 gaaccaaaat atacgctaga agaagctcgt atccacgatg ctagctactc agcaccaatt
    32041 tttgtaacct tccgtttgat caataaagaa acaagcgaaa tcaagaccca agaagttttc
    32101 tttggtgatt tcccaatcat gacagaaatg ggtactttca tcatcaatgg tggtgaacgt
    32161 attatcgttt ctcagttggt ccgctcacca ggtgtttact ttaacgacaa agtagacaaa
    32221 aatggtaagg tgggctatgg ttcaactgtt atccctaacc gtggagcttg gttggaactt
    32281 gaaagcgact caaaagatat cacctacact cgtatcgacc gtactcgtaa gattccattt
    32341 acaaccttgg ttcgtgctct tggtttctca ggtgatgatg aaatctttga tatctttggt
    32401 gacagcgaat tggttcgcaa cactgttgaa aaagatatcc acaagaatcc aatggactct
    32461 cgtacagacg aagccttgaa agaaatttac gaacgccttc gtccaggtga gcctaagacg
    32521 gctgaaagct cacgtagctt gcttgtagct cgcttctttg acccacgtcg ttatgacttg
    32581 gcagcagttg gtcgttacaa aatcaataaa aaactcaatg ttaaaacacg tttgctcaac
    32641 caaaccattg cagagccatt ggtagaccct gaaactggag aaatcttggt agaagctggt
    32701 acgattatga ctcgtagcgt gattgaaagc attgaaagcc atttggatgg cgacttgaac
    32761 aagattgtct acatcccaaa cgatgcagcc gttgtgactg agcctgttgt tcttcaaaaa
    32821 ttcaaggtta ttgctccaac tgatccagat cgcgtcgtaa cgatcattgg taatgctaac
    32881 ccagatgaca aggttcgtac ggtgactcct gcagatatcc ttgctgagat gagctacttc
    32941 ctcaacttgg ctgaaggact tggccgtgta gatgatatcg accaccttgg aaatcgtcgt
    33001 atccgtgcgg ttggtgaatt gcttgccaac caagtacgtt tgggactttc tcgtatggaa
    33061 cgtaatgtcc gtgaacgtat gtctgttcag gacaatgaag tcttgacacc acaacaaatt
    33121 atcaatatcc gtcctgtaac agctgcagtt aaagaattct ttggttcatc acagttgtca
    33181 cagttcatgg accaacacaa cccgctttct gagttgtctc acaaacgccg tttgtcagcc
    33241 ttaggacctg gtggtttgac tcgtgaccgt gctggatatg aagtacgtga cgtgcactac
    33301 actcactatg gtcgtatgtg tccaatcgag acacctgaag gacctaacat cggtttgatc
    33361 aataacttgt catcttatgg acacttgaac aaatatggtt ttgttcaaac accataccgt
    33421 aaggttgacc gtgaaacagg tgttgtcacg aacgaaattg tttggttgac agctgatgaa
    33481 gaagatgaat atactgtagc tcaggctaac tctcgtctga atgaagatgg aacctttgct
    33541 gagaagattg tcatgggacg tcaccaaggg gtcaaccaag agtatccagc taatattgtt
    33601 gactacatgg acgtttcacc aaaacaggta gttgccgttg cgacagcatg tattcctttc
    33661 ttggaaaacg atgactccaa ccgtgccctc atgggagcca atatgcaacg tcaggctgtg
    33721 ccattgatta atcctcaggc accttacgtt ggtactggta tggaatacca agcagcccac
    33781 gattctggtg cggctgtgat tgctcagtat gatggtaaag ttacttacgc agatgctgac
    33841 aaggtagaag ttcgtcgtga agatggttca ttggatgttt accacatcca aaaattccgt
    33901 cgttcaaact caggtactgc ttacaaccaa cgcactctcg taaaagttgg tgatgtcgtt
    33961 gaaaaaggcg atttcatcgc tgacggacct tctatggaaa atggagaaat ggcgcttgga
    34021 caaaacccaa tcgttgccta catgacttgg gaaggttaca acttcgagga tgccgttatc
    34081 atgagcgaac gcttggtgaa ggacgatgtc tacacatctg ttcaccttga agaatacgaa
    34141 tcagaaacgc gcgatacaaa gcttgggcct gaagaaatca ctcgcgaaat tccaaacgtt
    34201 ggtgaagatg ccctcaaaga ccttgacgaa atggggatta tccgtattgg tgctgaggtt
    34261 aaagaaggtg atattcttgt aggtaaagta acacctaagg gtgagaaaga tctttcagct
    34321 gaagaacgtc tcttgcacgc tatctttgga gacaagtctc gtgaagtgcg tgatacttct
    34381 cttcgtgtac cacacggtgc cgatggtgtc gttcgtgatg ttaagatctt tacacgtgta
    34441 aatggagatg agttgcaatc aggtgttaac atgttggttc gtgtttacat cgctcaaaaa
    34501 cgtaagatta aggtcggaga taaaatggcc ggacgtcacg gaaacaaagg ggttgtctct
    34561 cgtatcgttc ctgtagaaga catgccttac cttccagacg gaactccagt cgacatcatg
    34621 ttgaacccac ttggggtacc atcacgtatg aatatcggtc aggttatgga gcttcacctt
    34681 ggtatggcag ctcgtactct tggtattcac atcgctacac cagtctttga tggagcaagt
    34741 tctgaagatc tttggtcaac tgttaaagaa gcaggtatgg atagcgatgc caaaacgatc
    34801 ctttacgatg gacgtactgg tgaaccgttt gataaccgtg tttctgtcgg agtcatgtac
    34861 atgatcaaac tccaccacat ggttgatgat aaattgcacg cgcgttcagt cggaccatac
    34921 tcaactgtta cccaacaacc actcggaggt aaggctcagt ttggtggaca acgtttcggt
    34981 gagatggagg tttgggctct tgaagcctac ggtgcgtcaa atgtccttca agaaatcttg
    35041 acttacaagt cggacgatat caacggacgt ttgaaagcct atgaagctat tacaaaaggc
    35101 aaaccaattc caaaaccagg tgttccagaa tccttccgag ttcttgtcaa agaattgcaa
    35161 tctcttggtc ttgacatgcg tgtcctagac gaagatgacc aagaagtaga acttcgcgac
    35221 ttggatgaag gaatggacga agatgtcatc cacgtagatg accttgaaaa agcccgcgaa
    35281 aaagcagccc aagaggctaa agcagccttt gaagctgaag aagctgagaa agcaacaaaa
    35341 gcggaagcaa cagaagaagc tgctgaacaa gaataagcag ttcacttaga atagaaaggg
    35401 aagaaatagt ggttgatgta aatcgtttta aaagtatgca aatcacccta gcttctccaa
    35461 gtaaagtccg ttcatggtct tatggagaag tcaaaaaacc tgaaacaatc aattatcgta
    35521 ccttgaaacc agaacgtgaa ggactctttg atgaagtgat ctttggtcct acaaaagact
    35581 gggaatgtgc ttgtggtaag tacaaacgca ttcgttacag aggaattgtt tgtgaccgct
    35641 gtggggttga agtaacgcgt acgaaagttc gtcgtgagcg tatgggacat atcgaattga
    35701 aagctcctgt atctcacatc tggtacttca aggggattcc aagccgtatg ggcttgaccc
    35761 ttgatatgag ccctcgtgcc ctcgaggaag ttatctactt tgcggcttat gtggtgattg
    35821 atcctaagga tacaccactt gagcacaagt ctatcatgac agagcgcgaa taccgagagc
    35881 gcttgcgtga atatggttat ggttcatttg ttgccaagat gggtgcggaa gccatccaag
    35941 accttttgaa gcaagtagat cttgaaaaag aaattgctga actcaaagaa gaattgaaaa
    36001 cagctactgg acaaaagcgt gtcaaagcca tccgtcgttt ggatgttttg gatgcctttt
    36061 acaagtctgg aaacaaacct gaatggatga ttcttaacat ccttccggtt atcccaccag
    36121 atcttcgtcc aatgttgcag ttggatggtg gccgttttgc ctcatctgac ttgaatgacc
    36181 tttaccgccg tgttatcaac cgtaacaacc gtttggctcg tttgcttgag ttaaatgcac
    36241 caggtatcat cgttcaaaat gagaagcgta tgcttcaaga agcagttgac gctttgattg
    36301 acaatggtcg tcgtggtcgt ccaatcacag gaccaggtag ccgtccattg aaatcattga
    36361 gccacatgct taaaggtaaa caaggacgct tccgtcaaaa cttgctcggt aaacgtgttg
    36421 acttctcagg acgttcagtt atcgccgttg gtccaactct taagatgtac caatgtggtg
    36481 tgccgcgtga aatggcgatt gaactcttta aaccatttgt catgcgtgaa atcgttgccc
    36541 gtgatatcgt gcaaaacgtc aaagcagcta aacgcttggt ggaacgcgga gatgagcgta
    36601 tctgggatat ccttgaagaa gtgattaaag aacacccagt gcttttgaac cgcgcaccga
    36661 cccttcaccg tttgggtatc caagccttcg agccagtctt gattgatggt aaggctcttc
    36721 gcttgcaccc acttgtctgt gaagcctaca atgctgactt tgacggggac caaatggcca
    36781 tccacgtacc actttcagaa gaagcacaag cagaagctcg tatcctcatg ctagctgctg
    36841 agcacatctt gaacccgaaa gatgggaaac cggtagttac tccatctcag gacatggttt
    36901 tgggtaacta ctacttgacc atggaagaag ctggtcgcga aggtgaagga atggtcttca
    36961 aagaccgtga cgaagcggtt atggcttacc gcaatggtta tgttcacctc cactcacgtg
    37021 ttggtatcgc aacagacagc ctcaacaagc cttggacaga agagcaaaga cataaggtct
    37081 tgcttacaac agttggtaaa attctcttca acgatatcat gccagagggg ctaccatact
    37141 tgcaagaacc aaacaatgcc aacttgacag aaggtgttcc agctaaatac ttcttgccac
    37201 ttggtggaga tatcaaggaa gctatcagca atcttgagct caaccctcca ttcaagaaga
    37261 aaaaccttgg aaatatcatc gctgaaatct tcaaacgttt ccgtacgaca gaaacttctg
    37321 ccctacttga ccgcatgaag aacctcggtt accaccactc aactcttgca ggattgacag
    37381 tgggtattgc cgatatccca gtcgttgatg acaaggctga aatcattgaa gaatcacaca
    37441 aacgtgtcga acaaatcacc aaacaattcc gtcgtggtat gatcacagac gacgagcgtt
    37501 acaatgctgt tacagctgaa tggcgtgctg cccgtgaaaa acttgagaaa cgcttgattg
    37561 ctaaccaaga tcctaagaac ccaatcgtta tgatgatgga ctctggagcc cgtggtaaca
    37621 tctcaaactt ctcacagctt gccggtatgc gtggtctgat ggctgctccg aatggacgta
    37681 tcatggaatt gccaatcctt tcaaacttcc gcgaaggttt gtcggtattg gaaatgttct
    37741 tctcaactca cggtgctcgt aaaggtatga ccgatacggc ccttaagaca gccgactcag
    37801 gttacttgac tcgtcgtttg gtcgacgttg cccaagacgt tatcatccgt gaggacgact
    37861 gtggaactga ccgtggtctc ttgatccgtt ctatcgcaga aggaaaagag atgatcgagt
    37921 ctctcgaaga gcgtctcaac ggtcgttaca ctaagaaaac tgttaaacat ccagaaactg
    37981 gtgcagtgat tattggtcca aatgaattga ttacagaaga caaggcgcgt gaaattgtca
    38041 atgctggtgt ggaagaagtg actatccgtt ctgtatttac atgtaacact cgtcacggtg
    38101 tctgccgtca ctgttacggt atcaacttgg cgactggtga tgcggttgaa gttggtgaag
    38161 cagttggtac aatcgctgcc caatctatcg gggaacctgg tacacagctt acaatgcgta
    38221 ccttccacac aggtggggtt gcctcaaata ccgatatcac tcagggtctt cctcgtgtcc
    38281 aagaaatctt tgaagcccgc aatcctaaag gggaagcggt tattacagag gttaaaggac
    38341 aagttactgc tatcgaagaa gatgcatcaa ctcgtaccaa gaaagtcttt gttaagggtg
    38401 aaactggcga aggtgaatat gtcgttccat ttacagctcg tatgcgtgtc gaagttgggg
    38461 gccaagtagc gcgtggtgct gctctgacag aaggttctat ccaaccaaaa cgtctccttg
    38521 cagttcgtga tgtcttgtca gttgaaacgt accttctcgg tgaagtacaa aaagtttacc
    38581 gtagccaagg ggtagaaatc ggtgacaaac acatcgaggt aatggttcgt caaatgatcc
    38641 gtaaagtccg tgtcatggat ccaggtgata cagatcttct catgggtacc ctcatggata
    38701 tcaatgactt tacagatgcc aacaaagatg tccttatcgc aggtggagtt ccagcgacag
    38761 gtcgcccagt ccttatggga attaccaaag cctcacttga aacaaacagt ttcttgtcag
    38821 cggcttcctt ccaggaaaca actcgtgtcc ttactgacgc agctatccgt ggtaagaaag
    38881 accatctcct tggacttaaa gaaaatgtta tcatcggtaa gatcatccca gctggtactg
    38941 gtatggcccg ttaccgtaac cttgaaccac atgctgtcaa cgaagaagaa taccttaatc
    39001 ctccagtaga ggaagaagga aatgaagaaa caacagaagt agttgtggat actgccgttg
    39061 aaactgtgga agaaacagta gaataagaga gaatgagaga acccgagggt tctctcattc
    39121 tctcttctga aaactttctc catttttcca cgaaatgtgg taaaataaaa gaaagaagct
    39181 gacaaaggag acgggtatgg aacaaacatt ctttatcatc aaaccagatg gtgtaaaaag
    39241 agggctagtg ggtgaagtgt taaagcgcat cgaacaacgt ggatttacaa tcgaaaaatt
    39301 ggagtttcgt tcacaggttt cagaagagtt gattgaccag cactatcagg acttggttgg
    39361 tcagagtttt tacccaccga ttcgtgaatt catgacttca ggtccagttc ttgtgggtgt
    39421 catttctggt cccaaagtaa tcgaaacttg gcggaccatg atgggtgcaa ctcgtccaga
    39481 agaagcttta ccaggcacta ttcgaggtga ttttgcaaaa gctgctggag aaaatgagat
    39541 tatccaaaat gttgtacatg gttcagattc agaagagtca gctaagcgag aaattgctct
    39601 ttggttttaa gagtggattg gctcaatcaa ttggttaaaa gctcatttga atagaaagta
    39661 tagtcaatta gtttaagaca tgacgcatga tatcaaactt tttagttttt gatatggtgc
    39721 gttttttgat taagtgacta ttagtttgtc tcgctccgtt aggtgcgaga caaaaaaaac
    39781 acccgctatg cgggtgtgcg tcgaaggtta taccaaaaaa actccaaacg cgatacaata
    39841 aaggtgttca atccaattgt aaagcgaaag gagaaaaata tggcacaaaa ggttctttta
    39901 tcctgttctt gtttcaattg actatatttc tatacttttt cttcctttct ctgtgaaaat
    39961 cgcaagtttt tcccaccttt tctcaatact gaaacaacat ctttccagaa cttcaataat
    40021 ttctttttgt gttaaatcat tatttaacta aatctttgga ggcaatacgt tttcaaagct
    40081 acttttttac cataggtttt gagccttcaa cacagattac taagccaaaa atgctctaaa
    40141 tttttgaaat tctaacaaat tccactcaac tttatcatca tctttgactt tcacaatatc
    40201 ttcctcaaaa taacatggaa taaagatagt tttcttttaa aacggttgta ggaggaaaat
    40261 ctctcctaca ctattattga ttctgagaga aaattgcaaa atatcatcta ccaaactttc
    40321 aattattaag attttttgca gtttatctga attagaatcg caaggcttct caataatata
    40381 attgtcgaat tttttcattc taacttcctc tccattattt cttcctttat caagaaaaag
    40441 ttacctcaat attaataaag tacaattcta aattaagtac aattctaaat taactccaat
    40501 tcttgattca tgataatatt tcagtaactc atcgtgcaat taaaaacaag gatttttatt
    40561 tcagatgatc catcgtgaac acttcatcag ccatctccca aattgccgga ttatgtgttg
    40621 cgataatgat tagcctatta tcatctcgaa gagatagcaa aatctccata atcaactgag
    40681 aggttgctgg gtctattgaa gctgttggct catctgccag aataaagggt ggattcttta
    40741 agataatttt tgccaaggca acccgttgcg attctccgcc cgataactca aagatgcgct
    40801 tatctaggtc aagataaacc aggccgacct gttctaaagc ctgcttctgc ctcaaccgct
    40861 gttccgaccg actcaacttt tgaccaatga gacctagctt aaggttttct tcaatacttt
    40921 ggttttcaat taagccaaag ttctggaaga ggtagcccaa ttcgtgacgg aaaaaatcac
    40981 ttgatttata attggccaag tctttacctc ggtaaaaaat cgtcccatca taaggttcta
    41041 atttcccaat catgttcatc aaggttgttt ttccgctacc acttgaacca attaaggcat
    41101 agacttttcc agcctcaaat gtcattgaaa gattcgaaaa taactctcgt tctccaaaag
    41161 atttactcac ctgtttaagt tcaatcatat taacctccct tcaaaacaag catggacatc
    41221 ttgttttctt tctgcatttg gacatgtaac tgtaaaagag atagaccagt aaagagtaac
    41281 aagactaaga aagcaactcc tatctctacc tgaagaaata cactcgcaac aaatcccagt
    41341 aaaaacacac ccagttgagc aaagagataa gtgcgatgga tttctaagaa cctgagacct
    41401 gcaatgcgtt tgataaaaat ggcacgtcta aattcttcaa agtagagcct attcatagtg
    41461 ttaaacaaca agattgaagt tgcaatccca agcacagctc ctgctagcat tacccaacgt
    41521 tccctctgga tattatccaa taatgtgatg tagttgtggt aacctgtttg catttctgag
    41581 acccaatttt caatgccttg tctctggata agctcctggg catcagacaa ttgattaaag
    41641 agaacgtagt tctgtactgc gtctatccaa aacaaaatgg actgtggacc agttgattgg
    41701 ggtgttataa caatgatgat tggatctttc aaaaactgct ggtaagaaat aggggtcgta
    41761 ttatacacaa aacgatcctg acctgattct aaataaccta ccgtagcagt catttgctgt
    41821 cgttcatctt gactagatat gcgactggtt aagtcgtctt caaaaacaga tttataatgt
    41881 tcttcctctg aacggaggtg ttcaggcagc aataagacaa actcccctac atcaaggtga
    41941 ttcatctttt gactcatttt agttgaactg taggacttac tttttcagag ggctccaaat
    42001 tttattttca tttacttttt ttagcccata aaatatttac catacttata aaaagtagta
    42061 atactccaac taaactgact tgtaatgaat tatatgtatt ttctgaagtg ttaggtaaat
    42121 aattttgaga gtacccttct cgggcaatag taataatatt gtgtgtgttt atttgtttaa
    42181 ttccaatagt agtttgaatt ctctcttcca actcaattaa attaacatta tctatgaaac
    42241 tatcccaac
//
LOCUS       DAVHEW010000008        35668 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVHEW010000008 DAVHEW010000000
VERSION     DAVHEW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.008"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(1..523)
                     /locus_tag="VVL53_000412"
                     /pseudo
     CDS             complement(1..523)
                     /locus_tag="VVL53_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            518..772
                     /locus_tag="VVL53_000413"
     CDS             518..772
                     /locus_tag="VVL53_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU4182627.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1017..2351
                     /gene="trmFO"
                     /locus_tag="VVL53_000414"
     CDS             1017..2351
                     /gene="trmFO"
                     /locus_tag="VVL53_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU4182628.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2435..3172
                     /locus_tag="VVL53_000415"
     CDS             2435..3172
                     /locus_tag="VVL53_000415"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU4182629.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3181..3738
                     /gene="frr"
                     /locus_tag="VVL53_000416"
     CDS             3181..3738
                     /gene="frr"
                     /locus_tag="VVL53_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU4182630.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3798..4652
                     /gene="cvfB"
                     /locus_tag="VVL53_000417"
     CDS             3798..4652
                     /gene="cvfB"
                     /locus_tag="VVL53_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU4182631.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4661..4876
                     /locus_tag="VVL53_000418"
     CDS             4661..4876
                     /locus_tag="VVL53_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU4182632.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4962..5930
                     /locus_tag="VVL53_000419"
     CDS             4962..5930
                     /locus_tag="VVL53_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU4182633.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5908..6087)
                     /locus_tag="VVL53_000420"
     CDS             complement(5908..6087)
                     /locus_tag="VVL53_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182634.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6123..6623
                     /locus_tag="VVL53_000421"
     CDS             6123..6623
                     /locus_tag="VVL53_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182635.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            6626..6886
                     /locus_tag="VVL53_000422"
                     /pseudo
     CDS             6626..6886
                     /locus_tag="VVL53_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7253..8340)
                     /gene="ald"
                     /locus_tag="VVL53_000423"
                     /pseudo
     CDS             complement(7253..8340)
                     /gene="ald"
                     /locus_tag="VVL53_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8517..9086
                     /locus_tag="VVL53_000424"
     CDS             8517..9086
                     /locus_tag="VVL53_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182636.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9154..9804
                     /locus_tag="VVL53_000425"
     CDS             9154..9804
                     /locus_tag="VVL53_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4182637.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9788..12028
                     /locus_tag="VVL53_000426"
     CDS             9788..12028
                     /locus_tag="VVL53_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU4182638.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSLRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPTFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12427..13014
                     /locus_tag="VVL53_000427"
     CDS             12427..13014
                     /locus_tag="VVL53_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182639.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIATPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            13018..14199
                     /locus_tag="VVL53_000428"
     CDS             13018..14199
                     /locus_tag="VVL53_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182640.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTVNSHS
                     LESRLESRIAANERAINENEAKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEAIIPTLFVIGIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYSIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    14359..14490
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14506..15036
                     /gene="infC"
                     /locus_tag="VVL53_000429"
     CDS             14506..15036
                     /gene="infC"
                     /locus_tag="VVL53_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU4182641.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15069..15269
                     /gene="rpmI"
                     /locus_tag="VVL53_000430"
     CDS             15069..15269
                     /gene="rpmI"
                     /locus_tag="VVL53_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU4182642.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15321..15680
                     /gene="rplT"
                     /locus_tag="VVL53_000431"
     CDS             15321..15680
                     /gene="rplT"
                     /locus_tag="VVL53_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU4182643.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15738..16118
                     /locus_tag="VVL53_000432"
     CDS             15738..16118
                     /locus_tag="VVL53_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4182644.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16368..17168
                     /locus_tag="VVL53_000433"
     CDS             16368..17168
                     /locus_tag="VVL53_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU4182645.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDNHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            17131..18117
                     /locus_tag="VVL53_000434"
     CDS             17131..18117
                     /locus_tag="VVL53_000434"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU4182646.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLDLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLLIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKEFLR"
     gene            18159..20192
                     /locus_tag="VVL53_000435"
     CDS             18159..20192
                     /locus_tag="VVL53_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU4182647.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGAIGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKKSWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20253..21908)
                     /gene="pavA"
                     /locus_tag="VVL53_000436"
     CDS             complement(20253..21908)
                     /gene="pavA"
                     /locus_tag="VVL53_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU4182648.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            22013..22510
                     /gene="ybeY"
                     /locus_tag="VVL53_000437"
     CDS             22013..22510
                     /gene="ybeY"
                     /locus_tag="VVL53_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4182649.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22491..22886
                     /locus_tag="VVL53_000438"
     CDS             22491..22886
                     /locus_tag="VVL53_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU4182650.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22903..23802
                     /gene="era"
                     /locus_tag="VVL53_000439"
     CDS             22903..23802
                     /gene="era"
                     /locus_tag="VVL53_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU4182651.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23851..24675
                     /gene="mutM"
                     /locus_tag="VVL53_000440"
     CDS             23851..24675
                     /gene="mutM"
                     /locus_tag="VVL53_000440"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU4182652.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMERRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24675..25280
                     /locus_tag="VVL53_000441"
     CDS             24675..25280
                     /locus_tag="VVL53_000441"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU4182653.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELVTLREQ
                     LAQTEEIFFMDIPLLFEQDYSAWFTETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25267..26466
                     /locus_tag="VVL53_000442"
     CDS             25267..26466
                     /locus_tag="VVL53_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU4182654.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGFYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26466..26615
                     /gene="rpmG"
                     /locus_tag="VVL53_000443"
     CDS             26466..26615
                     /gene="rpmG"
                     /locus_tag="VVL53_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4182655.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26655..26888
                     /gene="secG"
                     /locus_tag="VVL53_000444"
     CDS             26655..26888
                     /gene="secG"
                     /locus_tag="VVL53_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU4182656.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26990..29344
                     /gene="rnr"
                     /locus_tag="VVL53_000445"
     CDS             26990..29344
                     /gene="rnr"
                     /locus_tag="VVL53_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU4182657.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29307..29774
                     /gene="smpB"
                     /locus_tag="VVL53_000446"
     CDS             29307..29774
                     /gene="smpB"
                     /locus_tag="VVL53_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU4182658.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29790..30650
                     /gene="tehB"
                     /locus_tag="VVL53_000447"
     CDS             29790..30650
                     /gene="tehB"
                     /locus_tag="VVL53_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU4182659.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPIAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30723..31676
                     /gene="coiA"
                     /locus_tag="VVL53_000448"
     CDS             30723..31676
                     /gene="coiA"
                     /locus_tag="VVL53_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU4182660.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31695..33497
                     /gene="pepF"
                     /locus_tag="VVL53_000449"
     CDS             31695..33497
                     /gene="pepF"
                     /locus_tag="VVL53_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU4182661.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYAYAYMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33499..34212
                     /locus_tag="VVL53_000450"
     CDS             33499..34212
                     /locus_tag="VVL53_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU4182662.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34279..35220
                     /gene="prsA"
                     /locus_tag="VVL53_000451"
     CDS             34279..35220
                     /gene="prsA"
                     /locus_tag="VVL53_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU4182663.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKSNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(35339..>35668)
                     /locus_tag="VVL53_000452"
     CDS             complement(35339..>35668)
                     /locus_tag="VVL53_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4182664.1"
                     /translation="AFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNS
                     QDNGRMESFFGILKSEMFYGYEQTFKSLNQLEQAIIDYIDYYNNKRIKIKLKGLSSVQ
                     YRTKSFG"
BASE COUNT        10716 a         6619 c         8022 g        10311 t
ORIGIN      
        1 cgatatttct acgactcatt ttgaacaact tcatatcatg actatagttc acagcgatat
       61 ccaaagaaac aattctccct tgacttgtga caatcgcttg agccttcata gcgtgaaatt
      121 tctttttacc agaatcattc gctaattgtt tttttagggc gattgatttt tacttccgtc
      181 gcatcaatca ttaccgtgtc ctcagaactg agaggagttc ttgaaaccgt aaaaccactt
      241 tgaacaagag ttatttcaac ccattggctc cgacggataa agttgctttc gtgaatacca
      301 aaatcagccg caatttcttc ataagttcga tattctcgca ctattgaaga gtgggcataa
      361 gaaggtcttc taggcttaat ttaggttttc gtccaccttt tgcgtgttta agttgataag
      421 ctgtttttaa tacagctaac atctcttcaa aagtggtacg ctgaacacca acaagacgct
      481 taaatcgtgc atcagttagt tgtttacttg cttcatcatt catagaacta ctataccata
      541 ttttgtttcg caggaagtct attggaaagt aagaaatatt gaagctgagg ctattagaag
      601 aaattgtgag cgtggtgcta ttttttcagg taaaataaaa tatcacgaag attcacagtt
      661 taaaggagat cactatgttg aatgttatgc tgttttagat aatacggtta tagcaagaga
      721 tagaataaca gtccctatcg atccgttatg tggaaaagat tttatagagt agcatataat
      781 tgattcttaa ctggaatact cactatctct ttacatcaag aaaatgacta aacagggaag
      841 tttgccttct tccctttttt tgttatacta gtagaagaaa aaatagaaag atttgtggga
      901 gtgaaaaagt cctgtggact ttttcagcct gagccaagaa actcgaaagc ttgtaagtct
      961 gattggcttt ttcaatgtga atcttaactt cacactccca aagaggtatt agtgtcgtgt
     1021 ctcaatctta tatcaatgtt atcggtgctg gtttggcagg ttctgaagca gcttaccaaa
     1081 tcgcagagcg tggtattcca gttaaactat atgaaatgcg tggtgtcaag tctacacccc
     1141 agcataaaac agacaatttt gctgagttgg tttgttccaa ttctttgcgt ggggatgctt
     1201 tgacaaatgc agttggtctt ctcaaggaag aaatgcgtcg cttgggttct gttatcttgg
     1261 aatctgctga ggctacacgt gttcctgccg gtggagccct tgcggtggat cgtgatggtt
     1321 tctctcaaat ggtgaccgaa aaagtggtca accacccctt gattgaagtg gtgcgtgatg
     1381 aaattacaga attgccaaca gatgttatta cagttgtggc gactggtccc ttgactagcg
     1441 atgccctggc tgaaaagatt catgccctta ataatggcga tggcttctat ttctacgatg
     1501 cagcagcgcc tattatcgat gtcaacacta tcgatatgag taaggtctat ctcaaatctc
     1561 gttatgataa gggagaagca gcctatctca atgctccaat gaccaagcaa gagtttatgg
     1621 atttccatga agccttggtc aatgcggaag aagcaccgct caattctttt gaaaaagaaa
     1681 agtactttga aggatgtatg ccaatcgaag ttatggccaa acgtggcatt aaaactatgc
     1741 tttatggccc tatgaagcca gtcggtcttg agtatccgga cgactacaca ggacctcgtg
     1801 atggagaatt taaaacacct tatgcggttg tgcaacttcg tcaggataat gcagctggta
     1861 gcctatacaa tattgttggt ttccagaccc acctcaaatg gggagaacaa aagcgtgtct
     1921 tccaaatgat tccgggtctt gaaaatgcgg agtttgtccg ttatggtgtc atgcatcgca
     1981 attcttacat ggattcacca aatcttcttg agcagactta ccgttccaag aaacaaccaa
     2041 atctcttctt tgctggtcag atgacggggg tggaaggcta tgttgagtcg gcggcttcgg
     2101 gcttagttgc gggaattaac gcagctcgtc tcttcaagga agaaagcgag gttattttcc
     2161 ccgagacgac agcgattgga agcttagctc attacattac ccatgccgac agcaaacatt
     2221 tccaaccaat gaatgtcaat tttgggatca tcaaggagtt ggaaggcgag cgtatccgtg
     2281 ataagaaggc tcgttatgaa aaaattgcag agcgtgccct tgccgactta gaggaatttt
     2341 tgactgtcta atttttttga aagaattgtt catgatacta taaaaatctt agaaattgtg
     2401 ataaaatagg taggatgaaa gaaggagagt gaaaatggcg aatcccaagt ataaacgtat
     2461 tttaatcaag ttatcaggtg aagcccttgc cggtgaacgt ggcgtaggga ttgatatcca
     2521 aacagttcaa acaatcgcaa aagagattca agaagttcat agcttaggta tcgaaattgc
     2581 ccttgttatt ggtggaggaa atctctggcg tggagaacct gcagcagaag caggtatgga
     2641 ccgtgttcag gcagattaca ctggaatgct tgggactgtt atgaatgctc ttgtgatggc
     2701 agattcattg caacaagttg gggttgatac gcgtgtacaa acagctattg ctatgcaaca
     2761 agtggcagag ccttatgtcc gtggacgtgc ccttcgtcac cttgaaaaag gccgtatcgt
     2821 tatctttggt gctggaattg gttcaccata cttctcgaca gatacaacag cggctcttcg
     2881 tgcagctgaa atcgaagcag atgccatcct catggctaaa aatggcgtcg atggtgtgta
     2941 caatgccgat cctaagaagg acaagacagc cgttaagttt gaagaattga cccaccgtga
     3001 tgttatcaac aaaggtcttc gtatcatgga ctcaacagcc tcaaccctct caatggacaa
     3061 cgacattgac ttggttgtct tcaacatgaa ccaatcaggc aacatcaaac gtgtcgtatt
     3121 tggtgaaaat atcggaacaa cagtttcaaa taatatcgaa gaaaaggaat aagaaagaat
     3181 atggctaacg taattattga aaaagctaaa gagagaatga cccagtctca ccaatcactt
     3241 gctcgtgaat ttggtggtat ccgtgctggt cgtgccaatg caagcttgct tgaccgtgta
     3301 catgtagaat actatggagt cgaaactcct cttaaccaaa tcgcttcaat tacgattcca
     3361 gaagcgcgtg ttttgttggt aacaccattt gacaagtctt cattgaaaga catcgaacgt
     3421 gccttgaacg cttctgatct tggtatcaca ccggctaatg acggttctgt gattcgcttg
     3481 gttatcccag ctcttacaga agaaactcgt cgtgaccttg ctaaagaagt gaagaaggtc
     3541 ggcgaaaatg ctaaagtggc tgtccgcaat atccgtcgcg atgctatgga cgaagctaag
     3601 aaacaagaaa aagcaaaaga aatcactgaa gacgaattga agactcttga aaaagacatt
     3661 caaaaagtaa cagacgatgc tgttaaacac attgacgaca tgactgctaa caaagagaaa
     3721 gaacttttgg aagtctaaaa ataaacagaa aaactcagtt ggcattgctg gctgagtttt
     3781 attcgaaaga aggaaatatg aatacaaatc ttgcaagttt tatcgttgga ctgatcatcg
     3841 atgaaaacga ccgtttttac tttgtgcaaa aggatggtca aacctatgct cttgctaagg
     3901 aagaaggcca acatacagta ggggatacgg tcaaaggttt tgcatacacg gatatgaagc
     3961 aaaaactccg cctgacaacc ttagaagtga ctgccactca ggaccaattt ggttggggac
     4021 gtgtcacaga ggttcgtaag gacttgggtg tctttgtgga tacaggcctt cctgacaagg
     4081 aaatcgttgt gtcactcgat attctccctg agctcaagga actctggcct aagaagggcg
     4141 accaactcta catccgtctt gaagtggata agaaagaccg tatctggggc ctcttggctt
     4201 atcaagaaga cttccaacgt cttgctcgtc ctgcctacaa caacatgcag aaccaaaact
     4261 ggccagccat tgtttaccgt ctcaagctgt caggaacttt tgtttaccta ccagaaaata
     4321 atatgcttgg ttttattcat cctagcgagc gttacgcaga gccacgtttg gggcaagtat
     4381 tagatgcgcg cgttattggt ttccgtgaag tggaccgcac tctgaacctc tccctcaaac
     4441 cacgctcctt tgaaatgttg gaaaacgatg ctcagatgat tttgacttat ttggaaagta
     4501 atggcggttt catgacctta aatgataagt catctccaga cgacatcaag gcaacctttg
     4561 gcatttctaa aggtcagttc aagaaagcct taggtggtct tatgaaggct ggtaaaatca
     4621 agcaggacca gtttgggaca gagttgattt agggaggctt atgagaaaat cattttacac
     4681 ttggctcatg accgagcgca atcctaaaag taacagtccc aaagcaattt tggcagacct
     4741 cgcttttgaa gaggcagctt ttccaaaaca tacagatgat tttgatgagg tcagtcgctt
     4801 tttggaggag catgccagtt tctcttttaa cctaggagat tttgacagca tttggcagga
     4861 atatctagaa cactagcatt tattcattgg gtttgggcta gtaatttctc catccctctg
     4921 ctataataaa aagaaataaa aggattagag aggttcttta tttgaaggaa cattcaatag
     4981 acattcaact gagtcatcca gatgacctgt ttcatctttt tggttccaat gaacgccatc
     5041 ttcgtttgat ggaagaagag cttgatgttg tgattcatgc tcgtacggag attgtccagg
     5101 ttttgggaga agagtctgcc tgtgaggaag cccgtcaagt tattcaggct ttgatggtct
     5161 tggtaaatcg tgggatgacc gttggtacgc cagatgtagt cactgcgatt agcatggtca
     5221 aaaacgatga aattgataag tttgtcgccc tttacgaaga agaaattatc aaggataata
     5281 ctgggaaacc gatccgcgtc aaaaccttag gtcaaaaact ttatgtggac agtgtcaaac
     5341 agcatgatgt gacctttgga attgggcctg cagggacggg gaagaccttt cttgcagtta
     5401 ctttggcagt gactgccctt aaacgtgggc aagtcaagcg gattatcttg acacgtccag
     5461 cggtggaagc aggagagagt ctaggttttc ttccgggtga tcttaaggag aaggtagatc
     5521 cttaccttag acctgtttat gatgccttgt atcagattct ggggaaagac caaacgactc
     5581 gtctcatgga gcgtgaaatt atcgaaattg cgccccttgc ctatatgcgt ggtcggacct
     5641 tggatgatgc ctttgtcatt ctcgatgaag cgcaaaacac gaccatcatg cagatgaaga
     5701 tgttcttgac gcgtttaggc tttcattcta agatgattgt caatggcgat atcagtcaga
     5761 ttgacctgcc acgcaatgtc aagtcaggtt tgattgatgc ccaagagaaa ctcaagaaca
     5821 tccatcagat tgactttgtt catttttcag ctaaggatgt ggttcgccat ccagttgtcg
     5881 ctcagattat ccgagcttat gaatattcta ctgaagttgc acacgactga ttttgaggaa
     5941 gttcgcctgc aaaagaatat acttttgaga gaggaaaatc cagttgtata ggctaaaggt
     6001 tttatccaaa ggtctgagac aacgattagg cacgatggaa agaactttta tgtggctgat
     6061 gacgatcagt gcatcttcct gtgtcataat cacagggcac aagaaagtag gaatttgaaa
     6121 agatgattga ccaactatct aagtattaca gttgtaggat actaactgaa aaggatattc
     6181 caagtatttt atctttatat gaaagtaatc ctctgtattt tcagcattgt ccaccagagc
     6241 caaattttgc aactgtaaaa gaggacatgc tttgtctacc tgaaggtaaa gctaaggctg
     6301 ataagttttt tgtaggattt tggaatggat ctgaccttgt ggctgttatg gattttgtct
     6361 atgcacatcc tgatgaggag actgttttta ttggtttgtt tatggttgat caagcctatc
     6421 agaggaaagg gattggtagt catattgtga cagaagcact agcttatttt gctaagaact
     6481 ttcgaaaggc acgtttggct tatgttaagg gaaatccgca atctcagcat ttttgggaaa
     6541 agcagggctt taaatcaatt ggatgcgagg ttaagcaaga actctatacg gttgttatcg
     6601 ttgaacagag cctagaagat tagaaatggc atcaagtaag aactatttgg aatttgtttt
     6661 ggaacaatta tcaggattag atgatgtgac ttaccgttct atgatggggg agtatattct
     6721 ttacttccgc ggcaagatta ttggcggcat ttatgacgat cgctttttag ttaaacccgt
     6781 gcaagcagtc ttagataaga ttgaccaatc ttcttttgag tttccataca aaggtgccaa
     6841 agaaatgatt tgagtggaag aacttgataa taagatgttt ctataagacc tgattttagc
     6901 tatgtataac caactgccaa cgcccaaacc taaaaagaaa aagcaagggt gaacgaagta
     6961 aaaaagaagt ctgctaaggc cctgtctttg cacgggtaaa attttatata taaaaagaag
     7021 ctgggactaa agagctcagc ttcctttggt ttatataatt gtcattacaa gacgaagtgg
     7081 ttgggcgaaa ctctgttgac tttattcaat ttagagtttc ttatgcacaa ttgagtctgg
     7141 aacgaaagtc tccagttgca aagtatacag tacaataaac caacgatgta atagctgatg
     7201 acacaaagca cagtgggtag gacttgtgaa gtcacccttt tcttttcaaa atttatacta
     7261 aatcattgat atcagtgtag tcacgattaa gtccttgagc aactggtagg ctagtcaagt
     7321 aaccttgata agtggtcaca ccttgacgca agccttcatc ttcagagatt gcttgtgcga
     7381 atcctttgcc agccaaagct tcgatataag gaagagtgac attggttagg gcgatggttg
     7441 aagtgcgggc aaccgcacca gggatattgg caacggcata gtggagaaca ccgtgttttt
     7501 catagacggg ttcatcgtgc gttgtcacac ggtcagctgt ttcgataacg ccaccttggt
     7561 caacagcaac gtcaacgata cagagcctgg acgcatttgt ttgaccatct catctgtcac
     7621 caattccggt gcttttgcac cagggatgag aatggctcca atcaccacat cagcatctct
     7681 cacacttgct tcaatgttga atgaattaga cataagagtt tgaatttgac ttccaaagac
     7741 ttcttctaga actgagagaa gcttggcact aatatctaaa atagtcactt gagcaccaag
     7801 accaagggcg atgcgggcag catgtgtacc gacgacacca tcaccgatga tagttacttt
     7861 tccttttgga acaccagagc caccagcttg cttagtaagg aagtgagctc cgatttgaac
     7921 agccatacga cctgcaacct cactcatagg aacgaggagc ggtagttgtc cttgattgtc
     7981 acgaacagtt tcataggcaa ttcctgttgt ttttgctgct aacatagcat ctgctaattc
     8041 tggagcagcg gccatgtgca agtaggtgaa gagaagaaga tcgtcgcgca agtaaccgta
     8101 ttcagaactt aaaggttctt ttactttcac aaccaactct gctgcccaag cttcaccagc
     8161 agtagcgaca atctcagctc cttgcttttg atagtcagca tcagtaaagc cagaaccgag
     8221 accagcattt gtttcgataa ggacacgatg accacgacta actaagctat gaacacctgc
     8281 aggtgtgagg gcgacacggt tttcgttatt tttaatttct tttgggattc cgattaacat
     8341 tgagataacc tacctttcaa ttgacggtct tgttttggtt gtcacattcg agttcataaa
     8401 tcaaaaatgt gacggtttca ttgtatatga aaccgcttca aaaatcaaga aaaacttgtc
     8461 atccaaattt ttttatgcta gactagtgaa aatcaagctc taatggaggg aaaagtatgg
     8521 aatcaatatt tgtgaaattt gcccagtatc cgtctataga aacggagcgt ttattgctta
     8581 gacctgtaac tttggatgat gcggaagcaa tgtttgacta tgcctcggac aagggtaata
     8641 cacgttacac ttttccaacc aatcaaagct tggaagaaac caagaataac attgctcagt
     8701 tctacttggc taatcccttg ggacgttggg ggattgaatt aaaagataac ggcaagttta
     8761 tcggaactat tgacttacac aagattgatc ctgttcttaa gaaggcagct attggctaca
     8821 ttatcaataa aaagtattgg aatcaaggat taacgacaga agccaatcgt gctgtgattg
     8881 agctagcttt tgagaagata gggatgaata agttgactgc ccttcacgat aaggctaatc
     8941 ccgcgtcagg aaaggtcatg gagaaatcag gcatgcgttt ttcccatgca gaaccatatg
     9001 cttgtatgga ccagcatgaa aaaggccgaa tcgtgacaag agttcattat gtcttgacca
     9061 aggaagacta ttttgcaaat aaataagcag ttgaaaagaa atttttcgac tgttttttct
     9121 tcctcttacg aataatctaa gagaggagaa aatatggaag caattatcga gaaaatcaaa
     9181 gagtataaaa tcatcgtcat ctgtactggt ctgggcttgc ttgtaggagg atttttcctg
     9241 ctaaagccag ctccacaaac acctgtcaaa gagacgaatt tgcaggctga agttgcagct
     9301 gtttccaagg actcatcgac cggaaaggaa gtgaacaagg aagagaagga agaacccctt
     9361 gaacaagatc taatcacagt agatgtcaaa ggtgctgtca aatcgccagg gatttatgac
     9421 ttgcctgtag gtagtcgagt caatgatgct gttcagaagg ctggtggctt gacagagcaa
     9481 gcagacagca agtcgctcaa tctagctcag aaagttagtg atgaggctct ggtttacgtt
     9541 cctactaagg gagaagaagc agttagtcaa cagactggtt cggggacagc ttcttcaaca
     9601 agcaaggaaa agaaggtcaa tctcaacaag gccagtctgg aagaactcaa gcaggtcaag
     9661 ggactgggag gaaaacgagc tcaggacatt attgaccatc gtgaggcaaa tggcaagttc
     9721 aagtcagtag acgagctcaa gaaggtctct ggcattggtg ccaagaccat agaaaaactt
     9781 aaagactatg ttacagtgga ttaagaattt ctctattccc ctaatttacc tgagttttct
     9841 gttactatgg ctttactacg ccattttctc agtatcctat tttgctttgt tgggctttgt
     9901 ttttctgcta gtctgtctct ttttccaatt tccttggaaa tctgctagca aagttctagt
     9961 gatttgtgga atctttggat tttggtttgt ttttcaaaat tggcaacaga gtcaagcgag
    10021 tcaaaatctg gcggattctg ttgaaggggt acggattttg cctgatacta ttaaggttaa
    10081 tggtgatagt ctatcctttc gtggcaagtc taacggtcgt gctttccaag tctattataa
    10141 actccagtcc gaggaggaga aagaagcctt tcaagcttta accgacctgc atgagatagg
    10201 actagaaggg aagctttcgg agccagaagg gcagagaaat tttggtggct ttaattacca
    10261 agcctatctg aagactcagg gaatttacca gactctcaat atcaaaacaa tccagtcact
    10321 tcaaaagatt ggcagttggg atataggaga aaacttgtcc agtttacgtc gaaaggctgt
    10381 ggtttggatt aagacgcact ttccagaccc tatgcgcaat tacatgacag gactcttgct
    10441 gggacatctg gacaccgact ttgaggagat gaatgagctt tattccagtc taggaattat
    10501 ccacctcttt gccctatctg gcatgcaggt aggttttttc atgaatggat ttaagaaact
    10561 tctcttgcga ttgggcttga cccaagaaaa gttgaaatgg ctgacttatc ccttttccct
    10621 tatctatgcg ggactaactg gattttcagc atcggttatt cgcagtctct tgcaaaagct
    10681 actggctcaa catggggtta agggcttgga taattttgcc ttgacggtgc ttgtcctctt
    10741 tattgtcatg ccaaactttt tcttgacagc aggaggagtc ttgtcctgcg cttatgcttt
    10801 tatcctgacc atgaccagca aagaagggga ggggctcaag gctgttgcta gtgaaagtct
    10861 agtcatctcc ttgggcatat tgcccattct atccttctat tttgcggaat ttcaaccttg
    10921 gtctatcctt ttgacctttg tcttttcctt tctatttgac ttgaccttct taccgctctt
    10981 gtctatttta tttgtccttt cctttctcta tccagtcatt cagctgaact ttatctttga
    11041 atggttagag ggcattattc gcttggtctc gcaggtggca aggagaccgc ttgtctttgg
    11101 tcaacccaac gcatggcttt taatcttatt gttaatttcc ttggctttgg tctatgattt
    11161 gaggaaaaac attaaaggat taacagtatt gagtttattg attacaggtc tctttttcct
    11221 taccaagtat ccactggaaa atgaaatcac catgctggat gtggggcaag gagaaagtat
    11281 tttcctacgg gatgtaactg ggaaaaccat tctcatagat gtaggtggta aggcagaatc
    11341 ttataagaaa atcgaaaaat ggcaagaaaa gatgacgacc agcaatgcac agagaagctt
    11401 gataccctat cttaaaagtc gaggagtagc taagattgac cagctgattt tgacaaatac
    11461 ggacaaggaa catgtcggag atttgttaga ggtgaccaag gctttccatg taggggagat
    11521 tttagtatca aaaggcagtt tgaagcagaa gcaatttgtg gtagaactac aggcgactca
    11581 aacaaaggtg cgtagtatga cagtagggga gaacttgccc atttttggaa gtcagttaga
    11641 agttctatct ctaaggaaaa tgggagatgg aggacacgat gataccctag ttttgtatgg
    11701 gaaattcttg gataagcaat ttctcttcac gggaaatttg gaggagaaag gagagaagga
    11761 cttgctgaag cactatccag acttgaaagt aaatgttttg aaagctagcc aacatggcaa
    11821 taaaaaatca tcaagtccaa cctttctaga aaaactcaaa ccagagctta ctcttatctc
    11881 agttggaaag agcaatcgaa tgaaactccc ccatcaggaa acattgacac gactggaagg
    11941 tatcaatagc aaagtttatc gaactgacca gcaaggagct atacgtttta aggggttgga
    12001 tagttggaaa atcgaaagtg ttcgatagga aagataaatg ttgtagatta gtgaaataaa
    12061 ctaaaaattt gttgcataat aatgataaaa atggtataat gaaaacgtat tcaatattga
    12121 ggatataaaa tcattaaaaa tcagcaaaag ttattttatt agttagttta taatctattg
    12181 gtcttcttca gtccagtgta tctgctaaga gtgttcttaa aagtatcggt atgattggaa
    12241 tatcttctat aagtctagta tgaatttcca tggatataga catactaacg ttccgtcatg
    12301 gtctcgttat tacagctatt ctgagtatcc agttggttct ggctggaact acggttgtta
    12361 tgagatcata aactattaca gcggaggcta ttaattctta aagagtgaga aaaaggaggg
    12421 ctagatatgt tgcagcttac tcatgtgacc ttaaaaacgc gacaagtcat cttgcaagat
    12481 gcggatttca cctttaaaaa gggtaggttt tatggtcttc ttgctatcaa tggctctgga
    12541 aagacgaccc tgttccgtgc cattagcaat ttaattccca taagtagtgg aaatatcgca
    12601 actcctcctt ctttatttta ttatgagagt attgaatggc tggatggaaa cttaagtggg
    12661 atggactacc ttcgtcttat caaaaacatc tggaagtcag acctaaactt gagagatgaa
    12721 atcgcctact gggaaatggc tgactatatc agtcttccca tccgcaagta ttccttaggg
    12781 atgaagcaac gcttggtgat tgctatgtat tttctcagtc aggccaaatg ctggctcatg
    12841 gatgagatta caaatggctt agacgagtat tatcgacaga agttttttga taggctagca
    12901 caaatcgata gacaagaaca gctggttctt ttaagttccc actataagga agagttggtt
    12961 gatatctgcg atagagtagt aaccattcat caggggcaga tagaagaggt ttagtttatg
    13021 aaagatgtta gtctattttt attgaaaaaa gttttcaaaa gtcgtttaaa ctggattatc
    13081 ttacttttat ttgcatctgt actcggtgtt accttttatt taaatagtca gactgtaaac
    13141 tcacacagct tggagagcag gttggaaagt cgcattgcag ccaacgagag agccatcaat
    13201 gaaaatgaag cgaaactttc ccaaatgtct gacaccagct cggaggaata ccagtttgct
    13261 aaaaataatt tagacgtgca aaaaaatctt ttgacgcgaa agacagaaat tctaacttta
    13321 ctaaaagaag ggcgctggaa agaagcctac tatctgcagt ggcaagatga agagaagaat
    13381 tatgaatttg tatcaaatga cccgactgct agctctggct taaaaatggg ggttgaccgc
    13441 gaacggaaga tttaccaagc cctgtatccc ttgaacataa aagcacatac tttggagttt
    13501 ccgacccacg ggattgatca gattgtctgg attttagagg ctatcatccc aaccttattt
    13561 gtgattggta ttatttttat gctaacacaa ctatttgcag aaagatatca aaatcatctg
    13621 gacacagctc acttatatcc tgtttcaaaa gtgacatttg caatatcctc tcttggagtt
    13681 ggagtgggat atgtaactgt gctgtttatc ggaatctgtg gcttttcttt tctagtggga
    13741 agtctgataa gtggttttgg acagttagat tatccctact caatttatag cttagtgaat
    13801 caagaagtaa ctattgggaa aatacaagat gtattatttc ctggcttact cttagctttc
    13861 ttagccttta tcgtcattgt ggaagttgtg tacttgattg cttacttttt caagcaaaaa
    13921 atgcctgtcc tctttctttc actcattggg attgttggct tattgtttgg catccaaaca
    13981 attcagcctc ttcaaaggat tgcacatctg attcccttta cttacttgcg ttcagtggag
    14041 attttatctg gaagattatc taagcagatt gataatgtcg atctaaattg gagcatgggg
    14101 atggtcttac ttccttgcct gattatcctt ttgttagtgg ggattctatt tatcgaaagc
    14161 tggggaagtt cacgtaaaaa ggaagttttt aaagtttagt tttcctatag ggaagctaaa
    14221 gaaaaactaa catagggaga gaatcaactt ggttctctct ttttgattcg aaaaccaaac
    14281 caaaatacaa acacaaactt ttcaaaaaat aactttttat cttgacaaga gctagaaaac
    14341 ttggtatcat ataaaagttg agaaaagcag aagtgagagc ttctcgcctt gtgacattaa
    14401 gttgcctggc cctacggatg aaaagtttcg aagaaacgct atcataacgt gcgggcttgt
    14461 atatttacga gtccgctatt gtttttctct aataaaacaa aagaggtgaa aaccatagca
    14521 aagcaagact tattcatcaa tgatgagatt cgtgtacgtg aagttcgctt gattggtctt
    14581 gaaggagaac agctaggtat caagccactc agtgaagcgc aagctttggc tgataacgct
    14641 aatgttgacc tagtattgat tcaaccccaa gccaaaccgc ctgttgcaaa aattatggac
    14701 tacggtaagt tcaaatttga gtaccagaag aagcaaaaag aacaacgtaa aaaacaaagc
    14761 gttgttactg tgaaagaagt tcgtctaagt ccaacaattg acaagggtga ctttgataca
    14821 aaacttcgta atgcacgtaa gttccttgaa aaaggaaata aagttaaggt atctattcgc
    14881 tttaagggtc gtatgattac ccataaagag attggtgcaa aagttttagc cgagtttgct
    14941 gaagcaactc aagatattgc catcatcgaa caacgtgcta aaatggatgg acgtcaaatg
    15001 ttcatgcaat tggcgccagc gactgacaaa aaataattgt cagaaagtta aataaaggag
    15061 aaaaaatcat gccaaaacaa aaaacacacc gcgcatcagc taaacgtttc aaacgtacag
    15121 gttctggtgg acttaaacgt ttccgtgctt acacttctca ccgtttccac ggaaaaacta
    15181 agaaacaacg tcgtcatctt cgtaaagcat ctatggtgca ttcaggagat tacaaacgta
    15241 tcaaagcaat gcttactcgc ttgaaataat agcttgactg taagtaaaca attctaggaa
    15301 atatttggag gaaataaaac atggcacgtg ttaaaggtgg cgttgtatca cgcaaacgtc
    15361 gtaaacgtat tcttaaatta gcaaaaggtt actatggagc taaacacatc ttgttccgta
    15421 ctgcaaaaga acaagtaatg aactcttact actatgcata ccgtgaccgt cgtcagaaaa
    15481 aacgtgactt ccgcaaattg tggattactc gtatcaatgc ggcagctcgt atgaacggac
    15541 tttcatactc acaattgatg catggtttga aattggctga gattgaagtt aaccgtaaaa
    15601 tgcttgctga cttggctgtt aacgatgcag tagctttcac agctcttgca gatgcagcta
    15661 aagcaaaact tggtaaataa caactgatga gactggaaca ggattgtcca gtctttttaa
    15721 aaataaagga gaaagatatg gcttcaaaaa tgctacacac ttgcttacga gtagaaaatc
    15781 ttgaaaaatc aatcgcattt tatcaagatg cttttggttt taaagaattg cgtcgcagag
    15841 attttccaga ccatgccttc acgattgtct atctaggtct tgagggtgat gactatgagt
    15901 tggagttgac ttataactac gatcacggtc cgtatgtggt tggtgatggg tttgcccata
    15961 tcgccctcag tacacctgat cttgaggcac ttcatcaaga acacagtgca aaaggctatg
    16021 aagtgactga gccaaatggt ctaccaggaa ctacaccaaa ctattacttt gtcaaagacc
    16081 ctgatggata taaggtcgaa gtcattcgtg aaaaataatt tcgtgcaagt taagtagaat
    16141 attcgttttc tacttgactt tttttagctg aaagagtata ctaataaaaa ttcaaccttt
    16201 aaggggagtc cagagagact cacaaggtgt cagataaaag aatagtgcaa ttttctagag
    16261 gagacttttt gagtgtgctc tcttgtgttg tacgatttta actgaggcct tgcactagca
    16321 aggtcttttc tttatctggt ccccttaaaa tttaaggagg aaaagttatg aatcccacat
    16381 gtaagaagcg tttgggtgtc attcggttgg aaaccatgaa ggtggttgca caagaggaaa
    16441 tcgcgccagc aatctttgaa ttagtcctag aaggagaaat ggttgaagcc atgcgagcag
    16501 gccaatttct tcatctgcgt gtaccggacg atgcccatct cttacgtcgt cctatttcaa
    16561 tttcgtctat tgacaaggca aacaagcagt gtcacctcat ttatcggatt gagggagctg
    16621 ggactgcaat tttttcaacc ttaagtcagg gagacactct tgatgtgatg gggcctcagg
    16681 gaaatggttt tgacttgtct gaccttgatg agcagaatca ggttctcctt gttggtggtg
    16741 ggattggtgt tccacccttg cttgaggtgg ccaaggaatt gcatgaacgt ggagtgaaag
    16801 tagtgacagt cctcggtttt gctaataagg atgctgttat tttgaaaacg gaattggctc
    16861 agtatggtca ggtctttgta acgacagatg atggttctta tggcatcaag ggaaatgttt
    16921 ccgttgttat caatgattta gatagtcagt ttgatgctgt ttactcgtgt ggggctccag
    16981 gaatgatgaa gtatatcaat caaacctttg ataatcaccc aagagcctat ttatctctgg
    17041 aatctcgtat ggcttgtggg atgggagctt gctatgcctg tgttctaaaa gtaccagaaa
    17101 acgagacggt cagccaacgc gtctgtgaag atggtcctgt tttccgcaca ggaacagttg
    17161 tattataagg agaaaattat gactacaaat cgattacaag tttctctacc tggtttggat
    17221 ttgaaaaatc cgattattcc agcatcaggc tgttttggct ttggacaaga gtatgccaag
    17281 tactatgatt tagacctttt aggttctatt atgatcaagg cgacaaccct tgaaccacgt
    17341 tttgggaatc caactccaag agtggcagag acgcctgctg gtatgctcaa tgcaattggc
    17401 ttgcaaaatc ctggtttaga ggttgttttg gctgaaaagc taccttggct ggaaagagaa
    17461 tatccaaatc ttcttattat tgccaatgta gctggttttt caaaacaaga gtatgcagct
    17521 gtttctcatg ggatttccaa ggcaactaat gtaaaagcta tcgagctcaa tatttcttgt
    17581 cccaatgttg accactgtaa tcatggactt ttgattggtc aagatccaga tttggcttat
    17641 gatgtggtga aagcagctgt ggaagcctca gaagtgccag tttatgtcaa attaaccccg
    17701 agtgtgaccg atatcgttac tgtcgcaaaa gctgcagaag atgcgggagc aagtggcttg
    17761 accatgatca atactctggt tggaatgcgc tttgacctca aaaccagaaa accaatcttg
    17821 gccaatggaa caggtggaat gtcaggtcca gcagtctttc cagtagccct caaactcatc
    17881 cgccaagtag cccaaacaac agacctgcct atcattggaa tggggggagt ggattcggct
    17941 gaagctgccc tagaaatgta tctggctggg gcatctgcta tcggagttgg aacagctaac
    18001 tttaccaatc cttatgcctg ccctgacatc atcgaaaatt taccaaaagt catggataaa
    18061 tacggtatta gcagtctgga agaactccgt caggaagtaa aagagtttct gaggtaaact
    18121 gcaatcaatc tgttcttgat tttttattag tttgtaatat gaatttagga gaattttggt
    18181 acaataaaat aaataagaac agaggaagaa ggttaatgaa gaaagtaaga tttatttttt
    18241 tagctctgct atttttctta gctagtccag agggtgcaat ggctagtgat ggtacttggc
    18301 aaggaaaaca gtatctgaaa gaagatggca gtcaagcagc gaatgagtgg gtttttgata
    18361 ctcattatca atcttggttt tatataaaag cagatgctaa ctatgctgaa aatgaatggc
    18421 taaagcaagg tgacgactat ttttacctca aatctggtgg ctatatggcc aaatcagaat
    18481 gggtagaaga caagggagcc ttttattatc ttgaccaaga tggaaagatg aaaagaaatg
    18541 cttgggtagg aacttcctat gttggtgcaa taggtgccaa agtaatagaa gactgggtct
    18601 atgattctca atacgatgct tggttttata taaaagcaga tggacagcac gcagagaaag
    18661 aatggctcca aattaaaggg aaggactatt atttcaaatc cggtggttat ctactgacaa
    18721 gtcagtggat taatcaagct tatgtgaatg ctagtggtgt caaagtacag caaggttggc
    18781 tttttgacaa acaataccaa tcttggtttt acatcaaaga aaatggaaac tatgctgata
    18841 aagaatggat tttcgagaat ggtcactatt attatctaaa atccggtggc tacatggcag
    18901 ccaatgaatg gatttgggat aaggaatctt ggttttatct caaatttgat gggaaaatag
    18961 ctgaaaaaga atgggtctac gattctcata gtcaagcttg gtactacttc aaatccggtg
    19021 gctacatggc agccaatgaa tggatttggg ataagaaatc ttggttttac ctcaagtttg
    19081 atggcaaaat ggctgaaaaa gaatgggttt acgattctca tagtcaagcc tggtactact
    19141 tcaaatccgg tggttacatg acagccaatg aatggatttg ggataaggaa tcttggtttt
    19201 acctcaagtc tgatggcaaa atggctgaaa aagaatgggt ctacgattct catagtcaag
    19261 cctggtacta cttcaaatct ggtggctaca tggcgaaaaa tgagacagta gatggttatc
    19321 agcttggaag cgatggtaaa tggcttggag gaaaagctac aaataaaaat gctgcttact
    19381 atcaagtagt gcctgtcaca gccaatgttt atgattcaga tggtgaaaag ctttcctata
    19441 tatcgcaagg tagtgtcgta tggctagata aggatagaaa aagtgatgac aagcgcttgg
    19501 ctattactat ttctggtttg tcaggctata tgaaaacaga agatttacaa gcgctagatg
    19561 ctagtaagga ctttatccct tattatgaga gtgatggcca ccgtttttat cactatgtgg
    19621 ctcagaatgc tagtatccca gtagcttctc atctttctga tatggaagta ggcaagaaat
    19681 attattcggc agatggcctg cattttgatg gttttaagct tgagaatccc ttccttttca
    19741 aagatttaac agaggctaca aactacagtg ctgaagaatt ggataaggta tttagtttgc
    19801 taaacattaa caatagcctt ttggagaaca agggcgctac ttttaaggaa gccgaagaac
    19861 attaccatat caatgctctt tatctccttg cccatagtgc cctagaaagt aactggggaa
    19921 gaagtaaaat tgccaaagat aagaataatt tctttggcat tacagcctat gatacgaccc
    19981 cttacctttc tgctaagaca tttgatgatg tggataaggg aattttaggt gcaaccaagt
    20041 ggattaagga aaattatatc gataggggaa gaactttcct tggaaacaag gcttctggta
    20101 tgaatgtgga atatgcttca gacccttatt ggggcgaaaa aattgctagt gtgatgatga
    20161 aaatcaatga gaagctaggt ggcaaagatt agtactataa gtgaatatga tttgagtgaa
    20221 tagtaagtta aaaatcctga tttcaagtaa aatcaggatt ttttcatgga tgcaattttt
    20281 ttggagtctg gtgtgacgcg gagggtcttt tgtcctgtgt aagtgacaaa gccgggtttt
    20341 ccaccagttg gtttattgag ttttttgact tcaatcatat ctacctgcac cagattcgac
    20401 aggcgccctt gagagaagta ggcagctaac tctgctgcgt ctgtcttgac tgcatcagat
    20461 gggtcaagat ttcctgagat gacaacatgg cttccaggaa tgtccttagc atggaaccaa
    20521 agttcctcct tgcgggccat tttaaaggtc aattcctcat tttgaagatt gtttcgtccg
    20581 acatagatga tggttttgcc atcgcttgct agatattgtt ctagtttttt gcgtttctgg
    20641 attttctccc gttgtcttct gcggataaaa cctgtttgaa tcaattcttc acggatttca
    20701 gcgatttctt ccagtccagc ttggttgagg acggtttcta cactttccag atagagaata
    20761 gtggctttgg tttcttcaat caaatcagtc aagtatttga cagcttcttt gagtttctga
    20821 taccgtttaa aatagcgttg ggcattctgg ttgggagtca gagccttatc aagcgcaatc
    20881 atgataggtt ggttggtata gtagttgtct aggataacct ggtcttggtc gttaggcact
    20941 tggtggagga aggttgtcag caattctcct ttttgacgaa attcttcagc gttgtctgtc
    21001 gccagtaact ctttttcctg ttttttgagt ttgtgtcggt ttttctgaag ttcattttca
    21061 acacgacgaa tcagttcact ggcctgctgt ttgacgcggt cgcgctcagc cttatcctta
    21121 tagtaggtgt ccaacaaatc agaaagattt gcaaaaggct ctcccacctg atttgcaaaa
    21181 ggaactggac tgaaggaagt ctcagtcaag catggcttgg tttcctgatt gaaaaaattt
    21241 cggaaagcgg aaagtttttc actaaccagt atcctttcca attcatttgc cgtatcgcgt
    21301 cccagacctt gaaagaggct ttgaagattt tttgctgtta gttcttgggt ttgcaggatt
    21361 tcaaagagct tttcatcctt gatagtaaaa ggattgagag attctgtact tggcggagcg
    21421 atataggtcg atcctggaag taaggtgcgg tagctatttt gtgaaaagcc gacgtgtttg
    21481 ataacttcga ggattttatg actgctttta tcgaccagta gaatattact gtgtttcccc
    21541 ataatttcga taatcaaggt agcctggata tggtctccaa tctcgttttt attggaaact
    21601 gtcatttcca caatacggtc attttccact tgctcaatcg actcaatcag ggccccctgc
    21661 aaatactttc tcaaaaccat gataaaggta gaaggttggg ctggattttc aaaagtcgtt
    21721 tgggtcagct gaatgcgtcc aaaaactgga tgggcagaaa ggagcaggcg atggctttgg
    21781 cgattgctgc ggatttgcaa gaccaactct tgttcaaaag gctgattgat tttctggatg
    21841 cgaccattca ctaattcgct tcgcaattcc tcaactatgt ggtgtaaaaa aaatccgtca
    21901 aatgacatcg ttctctcctt gtgattgtat tccatagtat tatatcaaaa aggtagaata
    21961 aaatcatgga aatgtggtat aataaagcca agtaaagaga aacgagaagc acatgtatat
    22021 tgaaatggta gatgaaactg gtcaagtttc aaaagaaatg ttgcaacaaa cccaagaaat
    22081 tttggaattt gcagcccaaa aattaggaaa agaagacaag gagatggcag tcacttttgt
    22141 gaccaatgag cgtagtcatg aacttaatct ggagtaccgt gacaccgacc gtccgacaga
    22201 tgtcatcagc cttgagtata aaccagaatt ggaaattgcc tttgacgaag aggatttgct
    22261 tgaaaatcca gaattggcag agatgatgtc tgagtttgat gcctatattg gggaattgtt
    22321 catctctatc gataaggctc atgagcaggc cgaagaatat ggtcacagct ttgagcgtga
    22381 gatgggcttc ttggcagtac acggcttttt acatattaac ggctatgatc actatactcc
    22441 ggaagaagaa gcggagatgt tcggtttaca agaagaaatt ttgacagcct atggactcac
    22501 aagacaataa acgaaaatgg aaaaatcgtg acctgatatc cagtttagaa tttgccatca
    22561 cagggatttt tactgctatc aaggaagaac gcaatatgcg aaaacatgca gtgacggctc
    22621 tagtggtcat ccttgcaggt tttggttttc aggtgtcacg aatcgaatgg ctctttctcc
    22681 tattgagcat tttcttggtg gtagcctttg aaattatcaa ttctgctatc gaaaatgtgg
    22741 tggatttagc cagtcactat cacttttcca tgctggctaa aaatgccaag gatatggcgg
    22801 ctggcgcggt attagtggtt tctctttttg cagccttaac aggcgcattg atttttctcc
    22861 cacgaatctg ggatttatta ttttaaacag taagaggaaa ttatgacttt taaatcaggc
    22921 tttgtagcca ttttaggacg tcccaatgtt gggaagtcaa cctttttaaa tcacgttatg
    22981 gggcaaaaga ttgccatcat gagtgacaag gcgcagacaa cgcgcaataa aatcatggga
    23041 atttacacga ctgataagga gcaaattgtc tttatcgaca caccagggat tcacaaacct
    23101 aaaacagctc tcggagattt catggttgag tctgcctaca gtacccttcg cgaagtggac
    23161 actgttcttt tcatggtgcc tgctgatgaa gcgcgtggta agggggacga tatgattatt
    23221 gagcgtctca aggctgccaa ggttcctgtg attttggtgg tgaataaaat cgataaggtt
    23281 catccagacc agctcttgtc tcagattgat gacttccgta atcaaatgga ctttaaggaa
    23341 attgttccaa tctcagccct tcagggaaat aacgtgtctc gtctagtgga tattttgagt
    23401 gaaaatctgg atgaaggttt ccaatatttc ccgtctgatc aaatcacaga ccatccagaa
    23461 cgtttcttgg tttcagaaat ggttcgcgag aaagtcttgc atctaactcg tgaagagatt
    23521 ccgcattctg tagcagtagt tgttgactct atgaaacgag acgaagagac agacaaggtt
    23581 cacatccgtg caaccatcat ggtcgagcgc gatagccaaa aagggattat catcggtaaa
    23641 ggtggcgcta tgcttaagaa aatcggtagc atggcccgtc gtgatatcga actcatgcta
    23701 ggagacaagg tcttcctaga aacctgggtc aaggtcaaga aaaactggcg cgataaaaag
    23761 ctagatttgg ctgactttgg ctataatgaa agagaatact aagtagaggt aggctcatgc
    23821 ctgcttcttg tttttacaga aggaggactt atgcctgaat tacctgaggt tgaaaccgtt
    23881 tgtcgtggct tagaaaaatt gattatagga aagaagattt cgagtataga aattcgctac
    23941 cccaagatga ttaagacgga tttggaagag tttcaaaggg aattgcctag tcagattatc
    24001 gagtcaatgg aacgtcgtgg aaaatatttg cttttttatc tgacagacaa ggtcttgatt
    24061 tcccatttgc ggatggaggg caagtatttt tactatccag accaaggacc tgaacgcaag
    24121 catgcccatg ttttctttca ttttgaagat ggtggcacgc ttgtttatga ggatgttcgc
    24181 aagtttggaa ccatggaact cttggtgcct gaccttttag acgcctactt tatttctaaa
    24241 aaattaggtc ctgaaccaag cgaacaagac tttgatttac aggtctttca atctgccctt
    24301 gccaagtcca aaaagcctat caaatcccat ctcctagacc agaccttggt agctggactt
    24361 ggcaatatct atgtggatga ggttctctgg cgagctcagg ttcatccagc tagaccttcc
    24421 cagactttga cagcagaaga agcgacagct attcatgacc aaaccattgc tgttttgagc
    24481 caggctgttg aaaaaggcgg ttccaccatt cggacctata ccaatgcctt tggggaagat
    24541 ggaagcatgc aggattttca tcaggtctat gataagactg gtcaagaatg tgtacgctgt
    24601 ggtaccatca ttgagaaaat tcaactaggt ggacgtggaa cccacttttg tccaaactgt
    24661 caaaggaggg actgatgaga aaaatcatcg gaatcactgg gggaattgcc tcaggtaagt
    24721 caactgtgac aaattttcta agacagcaag gctttcaagt agtggatgcc gacgcagtcg
    24781 tccaccaact acagaaacct ggtggtcgtc tgtttgaggc tctagtacag cactttgggc
    24841 aagaaatcat tcttgaaaac ggagaactca atcgccctct cctagctagt ctcatctttt
    24901 caaatcctga agagcaaaaa tggtctaagc aaattcaagg ggagattatc cgtgaggaac
    24961 tggttacttt gagagaacag ttggctcaga cagaagagat tttcttcatg gatattcccc
    25021 tactttttga gcaggactac agcgcttggt ttactgagac ttggttggtc tatgtggacc
    25081 gagatgccca agtagaacgc ttaatgaaaa gggaccagtt gtccaaagat gaagctgagt
    25141 ctcgtctggc agcccagtgg cctttagaaa aaaagaaaga tttggccagc caggttcttg
    25201 ataataatgg caatcagaac cagcttctta atcaagtgca tatccttctt gagggaggta
    25261 ggcaagatga cagagattaa ctggaaggat aatctgcgca ttgcctggtt tggtaatttt
    25321 ctgacaggag ccagtatttc tttggttgta ccttttatgc ccatcttcgt ggaaaatcta
    25381 ggtgtaggga gtgagcaagt cgctttttat gcaggcttag caatttctgt ctctgctatt
    25441 tccgcggcgc tcttttctcc tatttggggt attcttgctg acaaatacgg ccgaaaaccc
    25501 atgatgattc gggcaggtct tgctatgact atcactatgg gaggcttggc ctttgtccca
    25561 aatatctatt ggttaatctt tcttcgttta ctaaacggtg tatttgcagg ttttgttcct
    25621 aatgcaacgg cactgatagc cagtcaggtt ccaaaggaga aatcaggctc tgccttaggt
    25681 actttgtcta caggcgtagt tgcaggtact ctaactggtc cctttattgg tggctttatc
    25741 gcagaattat ttggcattcg tacagttttc ttactggttg gtagttttct atttttagct
    25801 gctattttga ctatttgctt tatcaaggaa gattttcaac cagtagccaa ggaaaaggct
    25861 attccaacaa aggaattatt tacctcggtt aaatatccct atcttttgct caatctcttt
    25921 ttaaccagtt ttgtcatcca attttcagct caatcgattg gccctatttt ggctctttat
    25981 gtacgcgact tagggcagac agagaatctt ctttttgtct ctggtttgat tgtgtccagt
    26041 atgggctttt ccagcatgat gagtgcagga gtcatgggca agctaggtga caaggtgggc
    26101 aatcatcgtc tcttggttgt cgcccagttt tattcagtca tcatctatct cctctgtgcc
    26161 aatgcctcta gcccccttca actaggattc tatcgtttcc tctttggatt gggaaccggt
    26221 gccttgattc ccggggttaa tgccctactc agcaaaatga ctcccaaagc cggcatttcg
    26281 agggtctttg ccttcaatca ggtattcttt tatctgggag gtgttgttgg tcccatggca
    26341 ggttctgcag tagcaggtca atttggctac catgctgtct tttatgcgac gagcctttgt
    26401 gttgccttta gttgtctctt taacctgatt caatttcgaa cattattaaa agtaaaggaa
    26461 atctagtgcg agtaaaaatt aatctcaaat gctcctcttg tggcagtatc aattacctaa
    26521 ccagtaaaaa ttcaaaaacc catccagaca agattgaggt tttaaagtat tgtcccaagg
    26581 aaagaaaagt aaccctacat cttgaatcta agtagatttt atggtaaaat aatagggatt
    26641 ttaaggagtt tgatatgtat aacctattat taaccatttt attagtatta tctgttgtga
    26701 ttgtgattgc aattttcatg caaccaacca aaaaccaatc cagcaatgta tttgatgcca
    26761 gttcaggtga tttgtttgaa cgcagtaaag ctcgcggttt tgaagctgta atgcagcgtt
    26821 tgacagggat tttagtcttt ttctggctag ccattgcctt agcattgacg gtattatcaa
    26881 gtagataaga aaataatggg caggactagg tctttgcctc tttttatttt taaaggatgt
    26941 ttgagaaggt tttacagtaa aagaaaatta aaaaatctag aaagaaaata tgaaagatag
    27001 aataaaagaa tatttacaag acaagggaaa ggtgactgtt aatgatttgg ctcaggcttt
    27061 gggaaaagac agttccaagg attttcgtga gttgattaaa accttgtcct taatggaaag
    27121 aaagcaccag attcgctttg aagaagatgg cagtctgacc ttggaagcca agaaaaaaca
    27181 tgagattacc ctcaagggga tttttcatgc ccataaaaat ggttttggtt ttgtcagcct
    27241 agaaggggaa gaggacgacc tttttgtagg gaaaaatgat gtcaactatg ctattgatgg
    27301 tgataccgtc gaggtagtga ttaagaaagt cgctgaccgc aataagggaa cagcagcaga
    27361 agccaaaatt attgatatcc tagaacacag tttgacaaca gttgtcgggc aaatcgttct
    27421 ggatcaggaa aagcctaagt atgctggcta tattcgttca aaaaatcaga aaatcagtca
    27481 accgatttat gttaagaaac cagccctaaa gttagaagga acagaagttc tcaaggtctt
    27541 tatcgataaa tacccaagca agaaacatga tttctttgtc gcgagtgttc tcgatgtggt
    27601 gggacactca acggatgtcg gaattgatgt tcttgaggtt ttggaatcaa tggacattgt
    27661 atccgagttt ccagaagctg ttgttaagga agcagaaagt gtgcctgatg ctccgtctca
    27721 aaaggatatg gaaggtcgtc tggatctaag agatgaaatt acctttacca ttgacggtgc
    27781 ggatgccaag gacttggacg atgcagtgca tatcaaggct ctgaaaaatg gcaatctgga
    27841 gcttggggtt cacatcgcag atgtttctta ttatgtgacc gaggggtctg cccttgacaa
    27901 ggaagccctt aaccgtgcga cttctgttta cgtgacagac cgagtggtgc caatgcttcc
    27961 agaacgacta tcaaatggca tctgctctct caatccccaa gttgaccgcc tgacccagtc
    28021 tgctattatg gagattgata aacatggtcg tgtggtcaac tataccatta cacaaacagt
    28081 tatcaagacc agttttcgta tgacctatag cgatgtcaat gatatcctag ctggcgatga
    28141 agaaaagaga aaagaatatc ataaaattgt atcaagtatc gaactcatgg ccaagcttca
    28201 tgaaacttta gaaaacatgc gtgtgaaacg tggagctctc aattttgata ccaatgaagc
    28261 gaagatttta gtggataaac aaggtaagcc tgttgatatc gttcttcggc agcgtggtat
    28321 tgccgagcgg atgattgagt cttttatgtt gatggctaat gaaacagttg ccgaacattt
    28381 cagcaagttg gatttgcctt ttatctatcg aattcacgag gagcctaagg ctgaaaaggt
    28441 tcagaagttt attgattatg cttcgagttt tggcttgcgc atttatggga ctgccagtga
    28501 gattagtcag gaggcacttc aagacatcat gcgtgctgtt gagggagaac cttatgcaga
    28561 tgtattgtcc atgatgcttc ttcgctctat gcagcaggct cgttattcgg agcacaatca
    28621 cggccactat ggactagctg ctgactatta tactcacttt accagtccaa ttcgtcgtta
    28681 tccagacctt cttgttcacc gtatgattcg ggattacggc cgttctaagg aaatagcaga
    28741 gcattttgaa caagtgattc cagagattgc gacccagtct tccaaccgtg aacgtcgtgc
    28801 catagaagct gagcgtgaag ttgaagccat gaaaaaggct gagtatatgg aagaatacgt
    28861 gggtgaagag tatgatgcag ttgtatcaag tattgtcaaa ttcggtctct ttgtcgaatt
    28921 gccaaacaca gttgaaggct tgattcacat cactaatcta cctgaatttt atcatttcaa
    28981 tgagcgtgat ttgactcttc gtggagaaaa atcaggtatc actttccgag tgggtcagca
    29041 gatccgtatc cgtgttgaaa gagcggataa aatgactgga gagattgatt tttcattcgt
    29101 acctagtgag tttgatgtga ttgaaaaagg cttgaaacag tctagtcgta gtggcagagg
    29161 gcgtggttca aatcgtcgtt cggataagaa ggaagacaag agaaaattag gacgctcaaa
    29221 tgataagcgt aagcattcac aaaaagacaa gaagaaaaaa ggaaagaaac ctttttacaa
    29281 ggaagtagct aagaaaggag ccaagcatgg caaagggcga gggaaaggtc gtcgcacaaa
    29341 ataaaaaggc acgccacgac tatacaatcg tagatacgct agaggcaggg atggtcctga
    29401 ctggaactga aatcaagagt gtacgagctg ctcgaattaa tctcaaggat ggctttgctc
    29461 aagtgaaaaa tggagaagtt tggctgagta atgttcatat cgcgccttac gaagagggca
    29521 atatctggaa ccaggaacca gaacgtcgtc gtaaactcct gctccataaa aagcaaattc
    29581 aaaaattgga acaagagacc aaagggacag gaatgacctt agttcccctt aaggtctata
    29641 taaaagatgg ctacgctaag cttcttttag gacttgccaa agggaagcat gactatgaca
    29701 aacgggagtc tatcaaacgt cgtgagcaaa atcgagatat cgcgcgtgtg atgaaagctg
    29761 ttaatcagcg ataaaaagag gaattgaaaa tggaaaaatt agttgcctat aaacgcatgc
    29821 ctttgtggaa taaacaaaca atgcctgaag ctgttcagca aaagcacaat acaaaagttg
    29881 ggacttgggg gaaaattact gtcttgaagg gagctctcaa gtttattgaa ttgacagaag
    29941 aaggggaagt tctagctgaa cacctctttg aagcaggggc agacaatcca atagcccaac
    30001 ctcaagcctg gcaccgagtg gaagctgcca cagatgatgt ggaatggtac ttggaatttt
    30061 attgtaaacc tgaggattat tttgctaaaa aatacaatac caatcctgtt cattcagagg
    30121 tcctagaggc catgcagaca gtgaaacaag ggaaagcttt ggatttgggt tgtggtcagg
    30181 ggcgtaattc tctttttcta gcccagcaag attttgatgt gacggctgta gatcaaaatg
    30241 gactagctct tgaaatcttg caaagcattg tggagcagga agatttggac atgcctgttg
    30301 gcctttacga tatcaattca gctagcattg aacaagaata tgattttatc gtttcaacag
    30361 ttgttctcat gtttctacaa gcggaccgca ttccagctat tattcaaaat atgcaggaga
    30421 aaaccagtgt tggtggttac aaccttatcg tttgtgccat ggacacggag gattatcctt
    30481 gctcggttaa cttcccattc acctttaaag aaggagaact ggcagactat tacaaggatt
    30541 gggaattggt taagtacaat gaaaatccag gccatttgca ccgtcgcgat gagaatggca
    30601 atcgtattca actacgcttt gcgaccttac tagctaagaa aatcaagtaa acacacatga
    30661 agattaggaa ttttcctgat cttttttctt ttttacgaat gatatagaaa aggagggaat
    30721 tcatgtttgt tgcgagagat gctaggggag aattggtaaa tgtgttagag gataaacttg
    30781 agaagcaagc atacacctgc ccagcttgtg gaggccagat ccatttgcgt caaggaccaa
    30841 gtgtacggac gcattttgcc cataaatcct taaaagactg tgattttttc tttgaaaatg
    30901 aaagtccaga acacctggcc aataaggaat ccctctatca ctggttgaaa aaagagacaa
    30961 aggttcaatt agagtacccg ctttcagaac ttaaacagat tgcggatgta tttgtaaatg
    31021 gcaatctagc tctagaagtt cagtgtagtc ccttgcctca gaaagtcctt aaagagcgaa
    31081 gtgagggcta tcgtagtcag ggttaccaag tactgtggtt gctgggtcaa aaactgtggc
    31141 tcaaggagcg tttgactcgt ctacagcaag gttttcttta tttcagtcaa aacatgggct
    31201 tttatgtttg ggaattagac aaggaaaaac aagttttaag actcaaatac ctgatttacc
    31261 aggatctccg cggtaaactc cattatcaaa tcaaggaatt ttcctatggt caaggtagtt
    31321 tattggaaat attgcgtctt ccctataaga gacaaaaaat atctcatttt acagtttctg
    31381 aggacaagga catctgtcgc tatatccggc aacaacttta ttatcaaaat ctcttttgga
    31441 tgaaagaaca agcagaagcc tatcaaaagg gagaaaatat cctgacttat ggactgaaag
    31501 aatggtatcc acaaattcga ccaatagtgg gcaaattttt ccagattgaa caagacttga
    31561 ctagctatta tcagcacttt tatacctatt accaaaaaaa tcctcaaaat gattggcaaa
    31621 agctttatcc accagccttt tatcagcaat atttcttgaa aaatatggta gaatagaaag
    31681 gatggaggaa tctaatggta ttacaaagaa atgaaataaa tgaaaaagat acatgggatc
    31741 tatcaacgat ctacccaact gaccaggctt gggaagaagc cttaaaagat ttaacagaac
    31801 aattggagac agtagcccag tatgaaggcc atctcttgga tagtgcggat aacctactag
    31861 aaatcactga attttctctt gaaatggaac gccagataga gaagctttac gcttatgctt
    31921 atatgaagaa tgaccaggat acacgtgaag ctaagtatca agagtactat gccaaggcca
    31981 tgacactcta cagccagtta gaccaagcct tttcattcta tgagcctgaa tttatggaga
    32041 ttagcgaaaa gcagtatgct gactttttag aagctcaacc aaagctgcag gtttatcaac
    32101 actattttga caagcttttg caaggcaagg atcacgttct ttcacaacgt gaagaagaat
    32161 tattggctgg agctggagaa atctttggtt cagcaagtga aaccttcgct atcttggaca
    32221 atgcggatat tgtgttccct tatgtcctag acgatgatgg taaagaagtt cagctatctc
    32281 atgggactta cacacgtttg atggagtcta aaaaacgtga ggttcgccgt ggtgcctatc
    32341 aagctcttta tgcgacttac gaacaattcc aacacaccta tgccaaaacc ttgcaaacca
    32401 atgttaaggt gcaaaattac cgtgctaaag ttcgtaacta caagagtgct cgtcatgcag
    32461 ccctcgcagc gaattttgtt ccagaaagtg tttatgacaa tttggtagca gcagttcgca
    32521 agcatttgcc actcttacat cgctatcttg agcttcgttc aaaaatcttg gggatttcag
    32581 atctcaagat gtacgatgtc tacacaccgc tttcatctgt tgaatacagt tttacctacc
    32641 aagaagcctt gaaaaaagca gaagatgctt tggcagtctt gggtgaggat tacttgagcc
    32701 gtgttaaacg tgccttcagc gagcgttgga ttgatgttta cgaaaatcaa ggcaagcgtt
    32761 caggtgccta ctctggtggt tcttatgata ccaatgcctt tatgcttctc aactggcaag
    32821 acaatctgga caatctcttt actcttgttc atgaaacagg tcacagtatg cattcaagct
    32881 atactcgtga aactcagcct tatgtttacg gggattactc tatctttttg gctgagattg
    32941 cctcaactac caatgaaaat atcttgacgg agaaattatt ggaagaagtg gaagacgacg
    33001 caacacgctt tgctattctc aataacttcc tagatggttt ccgtggaaca gttttccgcc
    33061 aaactcaatt tgctgagttt gaacacgcca ttcaccaagc agatcaaaat ggggaggtct
    33121 tgacaagcga tttcctaaat aaactctacg cagacttgaa ccaagagtat tatggtttga
    33181 gtaaggaaga caatcctgaa atccaatacg agtgggctcg cattccacac ttctactata
    33241 actactatgt atatcaatat tcaactggct ttgcggccgc ctcagccttg gctgaaaaaa
    33301 ttgtccatgg tagtcaagaa gaccgtgacc gctatatcga ctacctcaag gcaggtaagt
    33361 cggactatcc acttaatgtc atgagaaaag ctggtgttga tatggagaag gaagactacc
    33421 tcaacgatgc ctttgcagtc tttgaacgcc gtttaaatga gtttgaagcc cttgttgaaa
    33481 aattaggatt ggcataaaat ggttgaatcg tatagtaaga atgctaacca taacatgcgt
    33541 cgtcctgtcg tcaaagaaga aattgtagac ttgatgcgtc agcgtcaaaa gcaggtcaca
    33601 ggtttcttga aagaattgga agactttgcc cgcaaggaaa atattcctat tattccccat
    33661 gaaacggttg cttatttccg ttttcttatg gaaaccatgc agcctaaaaa tattctggaa
    33721 attgggacgg ctatcggttt ttcagctctc ttgatggctg aacatgcgcc aaatgctaag
    33781 attacaacta ttgatcgtaa tccagaaatg attggttttg ccaaggaaaa ttttgcccag
    33841 tttgacagtc gcaagcaaat cactctccta gagggagatg cggtggatgt cttatctaca
    33901 ctgacagagt cttatgattt cgtctttatg gattctgcca agtctaaata catcgtcttt
    33961 ctgccagaaa tcctcaaaca tttggaagtt ggtggtgtgg ttgtcttgga tgatattttt
    34021 caaggtggtg atgttgccaa ggatattatg gaagtccgtc gtggtcagcg aaccatttat
    34081 cgaggccttc aaaaattatt tgatgcaacc ttagacaatc cagaactcac cgcaacatta
    34141 gtgcctttag gagatggtat tctcatgctt cgtaaaaatg tagcagatgt tcaactgtct
    34201 gaaagcgaat gattttcaga aaaatttaag aaaaaatagt aaaatagata gagtaacact
    34261 tatctcaaag gagtagacat gaagaaaaaa ttattggcag gtgccatcac actattatca
    34321 gtagcaactt tagcagcttg ttcgaaaggg tcagaaggtg cagaccttat cagcatgaaa
    34381 ggggatgtca ttacagaaca tcaattttat gagcaagtga aaagcaaccc ttcagcccaa
    34441 caagtcttgt taaatatgac catccaaaaa gtttttgaaa aacaatatgg ctcagagctt
    34501 gatgataaag aggttgatga tactattgcc gaagaaaaaa aacaatatgg cgaaaactac
    34561 caacgtgtct tgtcacaagc aggtatgact cttgaaacac gtaaagctca aattcgtaca
    34621 agtaaattag ttgagttggc agttaagaag gtagcagaag ctgaattgac agatgaagcc
    34681 tataagaaag cctttgatga gtacactcca gatgtaacgg ctcaaatcat ccgtcttaat
    34741 aatgaagata aggccaaaga agttctcgaa aaagccaagg cagaaggtgc tgattttgct
    34801 caattagcca aagataattc aactgatgaa aaaacaaaag aaaatggtgg agaaattacc
    34861 tttgattctg cttcaacaga agtacctgag caagtcaaaa aagccgcttt cgctttagat
    34921 gtggatggtg tttctgatgt gattacagca actggcacac aagcctacag tagccaatat
    34981 tacattgtaa aactcactaa gaaaacagaa aaatcatcta atattgatga ctacaaagaa
    35041 aaattaaaaa ctgttatctt gactcaaaaa caaaatgatt caacatttgt tcaaagcatt
    35101 atcggaaaag aattgcaagc agccaatatc aaggttaagg accaagcctt ccaaaatatc
    35161 tttacccaat atatcggtgg tggagattca agctcaagca gtagtacatc aaacgaatag
    35221 tccaaatcaa tgagtcaggg aaaaaactcg acttcaggaa aaaatgaagc aaacattccc
    35281 acaataaaac gcatagtaca aggtttgtac tgccccccaa aaaagttaga caattaattt
    35341 atccgaagga tttagttctg tattgcacag agctaagtcc ttttagtttt atcttaattc
    35401 tcttattgtt gtaataatca atatagtcta taatggcttg ttccaattga ttaagtgatt
    35461 taaatgtttg ctcatagcca taaaacattt cggattttaa aatgccaaag aaagattcca
    35521 tcctaccgtt gtcttggctg tttcctttgc gtgacataga tgcttgaatt cccttactct
    35581 ctaggaagcg atgataagaa tcgtgttggt attgccagcc ttggtcacta tggagaatcg
    35641 tattctcgta gtgcttctct ttgaatgc
//
LOCUS       DAVHEW010000009        35543 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVHEW010000009 DAVHEW010000000
VERSION     DAVHEW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.009"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(117..1751)
                     /locus_tag="VVL53_000453"
     CDS             complement(117..1751)
                     /locus_tag="VVL53_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182665.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSSMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1732..2513)
                     /locus_tag="VVL53_000454"
                     /pseudo
     CDS             complement(1732..2513)
                     /locus_tag="VVL53_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2691..4145
                     /locus_tag="VVL53_000455"
     CDS             2691..4145
                     /locus_tag="VVL53_000455"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU4182666.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNSDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGSHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4235..6853)
                     /gene="alaS"
                     /locus_tag="VVL53_000456"
     CDS             complement(4235..6853)
                     /gene="alaS"
                     /locus_tag="VVL53_000456"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU4182667.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVAELKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(6875..7360)
                     /locus_tag="VVL53_000457"
     CDS             complement(6875..7360)
                     /locus_tag="VVL53_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU4182668.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7635..7874)
                     /locus_tag="VVL53_000458"
     CDS             complement(7635..7874)
                     /locus_tag="VVL53_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182669.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8127..9197)
                     /locus_tag="VVL53_000459"
     CDS             complement(8127..9197)
                     /locus_tag="VVL53_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4182670.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9194..9967)
                     /locus_tag="VVL53_000460"
     CDS             complement(9194..9967)
                     /locus_tag="VVL53_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4182671.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9964..10770)
                     /locus_tag="VVL53_000461"
     CDS             complement(9964..10770)
                     /locus_tag="VVL53_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4182672.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDEMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10751..11908)
                     /locus_tag="VVL53_000462"
     CDS             complement(10751..11908)
                     /locus_tag="VVL53_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182673.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12068..12973)
                     /gene="murB"
                     /locus_tag="VVL53_000463"
     CDS             complement(12068..12973)
                     /gene="murB"
                     /locus_tag="VVL53_000463"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU4182674.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKNGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13085..13837)
                     /locus_tag="VVL53_000464"
     CDS             complement(13085..13837)
                     /locus_tag="VVL53_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU4182675.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13847..14578)
                     /gene="budA"
                     /locus_tag="VVL53_000465"
     CDS             complement(13847..14578)
                     /gene="budA"
                     /locus_tag="VVL53_000465"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU4182676.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHRAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14720..15991
                     /locus_tag="VVL53_000466"
     CDS             14720..15991
                     /locus_tag="VVL53_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU4182677.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYSLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16011..16826)
                     /locus_tag="VVL53_000467"
     CDS             complement(16011..16826)
                     /locus_tag="VVL53_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU4182678.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVVQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16983..17636)
                     /gene="phoU"
                     /locus_tag="VVL53_000468"
     CDS             complement(16983..17636)
                     /gene="phoU"
                     /locus_tag="VVL53_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU4182679.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17648..18406)
                     /gene="pstB"
                     /locus_tag="VVL53_000469"
     CDS             complement(17648..18406)
                     /gene="pstB"
                     /locus_tag="VVL53_000469"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU4182680.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18419..19222)
                     /gene="pstB"
                     /locus_tag="VVL53_000470"
     CDS             complement(18419..19222)
                     /gene="pstB"
                     /locus_tag="VVL53_000470"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU4182681.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19233..20117)
                     /gene="pstA"
                     /locus_tag="VVL53_000471"
     CDS             complement(19233..20117)
                     /gene="pstA"
                     /locus_tag="VVL53_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU4182682.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20107..21024)
                     /gene="pstC"
                     /locus_tag="VVL53_000472"
     CDS             complement(20107..21024)
                     /gene="pstC"
                     /locus_tag="VVL53_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU4182683.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21024..21902)
                     /gene="pstS"
                     /locus_tag="VVL53_000473"
     CDS             complement(21024..21902)
                     /gene="pstS"
                     /locus_tag="VVL53_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU4182684.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22476..23780)
                     /locus_tag="VVL53_000474"
     CDS             complement(22476..23780)
                     /locus_tag="VVL53_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU4182685.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23781..24554)
                     /locus_tag="VVL53_000475"
     CDS             complement(23781..24554)
                     /locus_tag="VVL53_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU4182686.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRSLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24544..24822)
                     /locus_tag="VVL53_000476"
     CDS             complement(24544..24822)
                     /locus_tag="VVL53_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU4182687.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24822..25223)
                     /locus_tag="VVL53_000477"
     CDS             complement(24822..25223)
                     /locus_tag="VVL53_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU4182688.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25433..26050)
                     /locus_tag="VVL53_000478"
     CDS             complement(25433..26050)
                     /locus_tag="VVL53_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU4182689.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(26040..26813)
                     /locus_tag="VVL53_000479"
     CDS             complement(26040..26813)
                     /locus_tag="VVL53_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU4182690.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26824..27561)
                     /locus_tag="VVL53_000480"
     CDS             complement(26824..27561)
                     /locus_tag="VVL53_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU4182691.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27566..28696)
                     /locus_tag="VVL53_000481"
     CDS             complement(27566..28696)
                     /locus_tag="VVL53_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU4182692.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENMAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQIEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28780..29169)
                     /locus_tag="VVL53_000482"
     CDS             complement(28780..29169)
                     /locus_tag="VVL53_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU4182693.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29185..29568)
                     /locus_tag="VVL53_000483"
     CDS             complement(29185..29568)
                     /locus_tag="VVL53_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU4182694.1"
                     /translation="MLEVAYILVALAVIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29569..30357)
                     /locus_tag="VVL53_000484"
     CDS             complement(29569..30357)
                     /locus_tag="VVL53_000484"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU4182695.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYHQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30350..31285)
                     /locus_tag="VVL53_000485"
     CDS             complement(30350..31285)
                     /locus_tag="VVL53_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU4182696.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31415..31591)
                     /locus_tag="VVL53_000486"
     CDS             complement(31415..31591)
                     /locus_tag="VVL53_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU4182697.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31731..32438)
                     /locus_tag="VVL53_000487"
     CDS             complement(31731..32438)
                     /locus_tag="VVL53_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU4182698.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32589..33617
                     /gene="queA"
                     /locus_tag="VVL53_000488"
     CDS             32589..33617
                     /gene="queA"
                     /locus_tag="VVL53_000488"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU4182699.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34278..34667)
                     /locus_tag="VVL53_000489"
     CDS             complement(34278..34667)
                     /locus_tag="VVL53_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU4182700.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNSSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(34721..35413)
                     /locus_tag="VVL53_000490"
     CDS             complement(34721..35413)
                     /locus_tag="VVL53_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU4182701.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
BASE COUNT        10582 a         8119 c         6732 g        10110 t
ORIGIN      
        1 ttcaaaacat tcctccctat atttttgatt accaatctta atcatttaca actacattct
       61 aacaaactat aaaagcgttt gtcgaattga atttatcaag caagcaacca actatctcat
      121 cttttttcta tttctgccaa tatgcgtgac aggtaataat gatagccaaa aatagcaaga
      181 gcaagcaaga cgataagagc tcctactccc aagttgatgg caaggatagg ggagagagac
      241 tgaaccaaga atatgctccc aattacaagg gccatcagga ttgcactata aataaacaat
      301 aaaactatgg cgactatgcc atttgaacga ttcaccaggt ccgtaatgct actccaattg
      361 gttgacagat ttttaacgtc cttaaagtaa tggtggcaag aaaggatgac actggcaatg
      421 atccagacta caagaaggta aatcatcgaa aggatggaca agcctagata tagagaaaga
      481 ccgagcaaag tcagaattgg taaaaaggac tgaacagcaa agataatcca aaatttcact
      541 ttcacataac gagcaaagtc aaagggtaaa ctcttaagaa aatcaacatt ttccctctcc
      601 aaggacaagg caattgaatg caggctggtg atattgttat tgacaactgc tataaagaga
      661 gctataaaaa acaagggtaa ccagtatgga ggatgaatgt ctggaactat ctgagaatct
      721 cggattttgg aaatcagacc gatcatcatg agataaggaa ggaaagcact tgtaaaaagc
      781 actgtaacca cgccagtacc ctgtcccaag agggtgaggt ggtagcgtaa aaccatgcgg
      841 aaaaatccct ttttagtggt tgaaattctc tccttgctgc gacgttcttt tttgaccttc
      901 tcctcactat taagcaggat cacgtcataa aaacgaggaa ggaccttctt tttggtcaga
      961 taaagcagga agagagttag tcctatccaa gcgagcagac ccactaaggc ttctgtcgaa
     1021 aaaggctcca ctgctatttt gtaaaagata tgaagaggat aaaggagaaa tggaatgtct
     1081 ctaactttgt caacaatact tccaaaagtc gactgaagaa agaagataaa tattaaaggt
     1141 atgagaactc ctatcccaat catcacattc gaaaaaatag actgatactt tctaaagacc
     1201 ctagtctgag ccaagaaatg cactgccact accatcacta gagccacaga gacaaataat
     1261 aaggtcaagg acagtagcat caaaggcaaa cccagccata gagaaggagc tagcctaatg
     1321 tagaggacta gaaaataagc taggattggt acaattccag ttagagctgg caaaaggaca
     1381 gacagtcctt tagcaattat aatctctgat tctttaaagg catagggcct atacgatatc
     1441 aaatccttac tctcataaaa gacattgtaa aaggccgtta aagaagttga aaaggcaatc
     1501 actagtaaaa tagcaatcat cgagctaaaa taaataggta tttcctcaaa aggaaaatga
     1561 atggctatat tactaaaaca gatgatcatc aagagactgg aaaaaatgta agaacttaag
     1621 actctagcgg aaacatttac ttttttccca ggattcttag cctgcttctt tcgtagatta
     1681 gccagattag cttcttgaga tgaataaagg atattgatat caactaattt tttaatgacc
     1741 ttgagacgca tccgcaacct cctcttttct accagcaaga ctaaggtaga tactttccaa
     1801 agactggtct gggtaatctt ttctcaagtc ctctacacta ccacaataaa tcaaatgccc
     1861 ctttttcaaa atggcaatcc gatcacagac ttgctctgcc acctctagga catgagttga
     1921 aaacaagact gtcttccctt tttgtgcatg ttccttcatc atctgtttca aatcaaaggc
     1981 agcctgggga tccaaaccag tcaagggttc gtccaagacc caaatatcag gatcagacaa
     2041 gagtgccccg atgacaaaaa ctttctgacg cattccatga gaaagggttt caataacctg
     2101 ataacgattt tcagcaaaat caaaaacgtt caatagccta gctagactag ctctgacttg
     2161 gagactagcc tagctagact agtctccaag tcagagctag tcagatcata ggatgaggcg
     2221 atcaattccc aaaattcatt ggccgttaaa cgtaaaaata agtcaggcga gtctgctacg
     2281 tagccaatct ttcgtttgat cgccaagcga ttttccgata actcctgacc atctaccaaa
     2341 atgcgaccac tgctgggtga aatgatactg actagggatt ttatggtggt cgattttcca
     2401 gccccattat gaccaatcaa gcccataatc tctccatttt caatctgcaa attgagatta
     2461 ctcaaggcct ctttatcacc atacaactta ctaacatttt gaaattcaat catgggatga
     2521 ctcctatctt tatctatatt acatactatt atactagcac tttgatacaa aaaatcaata
     2581 ctttatttta agcagttaaa atagtttcct tctatcttac gcaaacgttt gcgctaatag
     2641 atactttatg atagaataaa gaacaagatt gacaagtaag aggaaacatt atgcaaaatc
     2701 aaacactcat gcaatacttt gaatggtatc tgccccacga cggtcaacac tggacgcgtc
     2761 tggctgaaaa tgctccacac ctagctcatc tggggatcag tcacgtctgg atgccaccag
     2821 ccttcaaggc aaccaacgaa aaagatgtcg gctatggggt ctatgactta tttgacttag
     2881 gagagttcaa ccaaaaaggg actgtccgca ccaagtatgg tttcaaagaa gactatcttc
     2941 aagccattca agcccttaaa gcacagggaa ttcaacctat ggccgatgta gttctcaacc
     3001 acaaggctgc tgccgatcac agggaagcct ttcaggttat cgaagttgat cctgtagacc
     3061 gtacagttga acttggagaa cccttcacca tcaatggctg gactagtttt accttcgatg
     3121 gtcgccaaga tacctataat ggcttccact ggcattggta ccacttcacc ggtacagact
     3181 acgatgccaa acgcagtaaa tctgggattt atctgatcca aggggacaac aagggctggg
     3241 ctaacgagga attggtcgat aacgaaaacg gaaactacga ctacctcatg tatgccgacc
     3301 tagactttaa acatcctgaa gtcatccaaa acatctatga ctgggctgat tggttcatgg
     3361 aaacgactgg tgtagctggt ttccgtttgg atgccgttaa gcatattgac tctttcttta
     3421 tgcgcaactt catccgcgat atgaaggaaa aatacggtga cgatttctat gtttttggtg
     3481 aattttggaa ctcagacaag gaagccaatc tggactatct cgaaaaaacg gaagaacact
     3541 ttgaccttgt cgatgttcgt ctccaccaga atctctttga agccagtcaa gctggcgcaa
     3601 actatgacct tcgtggcatt ttcacagata gcctggttga actcaagcct gacaaggctg
     3661 tgacttttgt cgacaaccac gatacccaac gaggacaagc ccttgagtct accgttgaag
     3721 aatggttcaa gccagcagcc tatgccctca ttttgttacg ccaagacggc cttccatgtg
     3781 tcttttacgg agactactat gggatttcag ggcagtatgc tcaacaagat ttcaaagaaa
     3841 tccttgaccg cctcctagcc atccgaaaag acttggccta tggagaacaa aatgactact
     3901 ttgaccatgc taactgtatc ggttgggtac gttcaggtgc tgaaaatcaa tccccaatcg
     3961 cagtccttat ctcaaatgac caagaaaaca gcaagtcaat gtttgtcggt caagaatgga
     4021 ctaatcaaac ctttgtagat ttacttggta gccaccaagg tcaagttaca attgatgagg
     4081 aaggttatgg acaattccct gtctcagcta gatccgtaag tgtctgggca gtcaatacca
     4141 tctaatagct cataataacc aagctaggtc caagcggatt tggctttttt gtattcacaa
     4201 aaagacctac ccaaatggat agatctttac ttgattacaa tttacctgct actgcatcca
     4261 acaattcttg gatcttaggt tggttgcttc ctcctgccat ggccatatct ggtttaccac
     4321 caccacgtcc atcgatgatt ggtgctaatt ctttgacaag gtttcctgca tgaaggtctt
     4381 ttgtcttgct tgctacaagg acattgactt tgtcaccgat agcggcaact aggacaagaa
     4441 gatcagagta gtctttttgt ttccagttat ctgcaaaagt acgaagggca ccggcatcgg
     4501 atacagacac ttgactagca atgtaacgat gaccgttgac ttccttaaca tctttgaaga
     4561 tatcgcctgc ggctgcagct gcggcttttt ctttcaactc agcattttct ttttgaagtt
     4621 gacgaagttg ttcttgaagt ccttctacct tgtgaggtac ttccttgact tgaggtgctt
     4681 tcaaggttgc tgcgatagct ttaagagcat cctcttgttc acgataggct tcaaaggctt
     4741 ccttaccagt cactgccaag atacggcgag ttcctgaacc gattccttct tctttgacaa
     4801 ttttgaagag accaatctca gaagtgttgt caacatgagt accaccacaa agttcaatag
     4861 agtagtcacc gatagtcacg acacgaactt ccttgccgta tttctcacca aagagggcca
     4921 tagctcccat ttctttagca gtgtcaatat ccgtttcaac tgtcttcact tcaagtgctt
     4981 cccaaatttt ctcgttaact tgctgttcaa tcgcacgaag ttcctcagca gttactgctt
     5041 ggaagtgggt aaagtcaaag cgaaggaatt caacttcgtt aagagatcct gcctgtgttg
     5101 cgtggtttcc aaggatattg tgaagggcag cgtgaagcaa atgagtcgca gtgtggtttt
     5161 tcatgacacg gtgacggcga ttgctatcaa ttgccaaggt atattcttgg ttcaaggcaa
     5221 gcggtgcaag gacttcaact gtatgaaggg cttgaccatt tggggctttc tgaacattgg
     5281 tcacagtagc cacaacctta cctgactcat ccaagatttg tccgtagtca gctacctgtc
     5341 cacccatttc agcataaaat gacgtttccg caaagataag agaggcagtt ccttctgaaa
     5401 cagctcctac ttctgcattg tcagcaacga tagccaccaa tttagaagac aattggctag
     5461 cattgtagtt gaagacactt tctacagtga tgttttgaag agtttcattt tgcataccca
     5521 ttgagccacc cttgacagct gacgcacgcg cgcgttcttg ttgttctttc atggctgctt
     5581 caaaaccttc acggtctaca gtcataccag cctcttcagc aatttcttca gtcaattcaa
     5641 ctgggaatcc gtatgtatca tagagtttaa agacatcttg accagcaatg acagattgac
     5701 ctttttcttt caactctgcc acaatgcctt gggcaaagtg ttgacctgag tgaagggtac
     5761 gggcaaatga ttcttcttcg ctcttaacga ttttctcaat aaagtcacgt ttctcaagca
     5821 cttctgggta gtagctttcc atgatttttc caacagttgg aacaagtttg taaaggaaag
     5881 gctcgttgat acccaatttt tgaccatgca tagaagcacg acggagaaga cgacgaagaa
     5941 cataaccacg accttcattt cctggaaggg caccatcacc gatagcaaat gaaagagaac
     6001 gaatgtggtc tgcgataacc ttgaagctca tgttgtcgcc atcttggtca taaaccttac
     6061 cagacaattt ctcgacttca cggataatcg gcatgaagag gtccgtttca aagttggtct
     6121 tagccccttg gataacagcc accaaacgct ccaaaccagc gcccgtatca atgttcttat
     6181 gtggcaattc cttgtattcg ctacgaggaa cagcagggtc tgcgttaaat tgtgacaaaa
     6241 caatgttcca gatttcaata taacggtcgt tttcaatatc ttctgcaagc aggcgaagac
     6301 cgatattttc tgggtcaaag gcttccccac ggtcaaagaa gatttctgta tctggtccag
     6361 aaggtcccgc accgatttcc cagaagttgt cctcaattgg aatcaagtga cttggatcca
     6421 ctcccacttc aatccagcgg ttgtaagaat ctttatcgtc tggatagtag gtcatgtaaa
     6481 gtttttcagc agggaaatca aaccattcag ggcttatcaa aagctcataa gcccaagtga
     6541 tagcttcgtc acggaagtaa tccccgatag agaagttccc caacatttca aacatggtat
     6601 ggtgacgcgc agtcttccct acgttttcga tgtcgttggt acggatagcc ttttgggcat
     6661 tggtaatacg tggattttca gggataatgg tcccgtcaaa gtatttctta agggttgcta
     6721 ccccagagtt gatccacaaa agagttgggt catttacagg aaccaaactt actgatggtt
     6781 ctactgagtg acctttggtc gcccagaaat caagccacat ttggcgtact tgtgcactag
     6841 atagttgttt catattgtct ccttatttac ttgtttaatg tgattggctt tccagcattt
     6901 ccacatagtc aatcgcgaca cagagggaaa tgactaggtc tgcataagcg tcttcaagaa
     6961 ccgttacggt ataggtagag gtcagatgga agagttcctt cttaatttcc gcaatcaact
     7021 gatcgcgatc atccagcaat ttgaaattca aatcccagat attgccctcg atacgaagac
     7081 ctagattatc aaactcatac ttatctcgcc aaaaggtcaa cttcttacga atgacaaaac
     7141 tcgagccatc ccgaagctga atctcaaaac gaggaagcaa ggtcaagatt tctttactga
     7201 tctgactgac ttgttcacca gccgcatcat agatggtaaa agttttggga atcttaaaaa
     7261 atgatccctc cacctgatag gcaatttctc ccctgtcatc cttgatagcg aagcgttcgc
     7321 ctccaagacg aaacttttgt ttgacaagaa atgttttcat caacacctcc aaaaatcaaa
     7381 agacaagctc atatcacgaa gggcgaaaaa ccgcggtacc accttcattc aatgaacttg
     7441 tcattctctt gttcttatgc aattgtatga ttgagtagca tgacttccta gcttagatgg
     7501 ctcgcagcac cgccatttct ctggactaag acaagtgaaa atcaattctc aactttctta
     7561 ttatatcgtt tttttaatct tgcgtcaact ggaaatgatc tccgttgaat tagaccaatt
     7621 ccctacatct ctgattactt tttcaggata tattttttct tactgccgtt tttcttttta
     7681 tcccaaattt tcatattact aaacacagct actagaatat ttccaaatat aaaggtgcct
     7741 atcacccaat atatggactc agttgttagg tattgtcgat ccaagccatc ctttaaatgg
     7801 aatagtatag cagtttggtt aacaatcata aaggttggcc agaaactttt gttgaaaaaa
     7861 gtagacattt tcattatttg ttgccgcttt ctgtaaggtt aatactcaat aaaaatcaaa
     7921 aagcaaacta ggaagctagc ctcaagctgt acttgagtac ggcaaggcaa cgctgacgtg
     7981 gtttgaagag tataggctta gtatactact aggcaagcaa ataagcaaat aaacaactag
     8041 aatagaaaaa gatagggctc taaaaactga cttctattcc ttaaaaacga accagcttga
     8101 ctgattcgtc ttcttacgtt tatctcctac ttccgataca ttttaaactg taggaagagg
     8161 tcgctatatt tccctgtcca tttatggtca aatttctcat aaacttctag gtgtttcatg
     8221 gtttcaacat cgggatagaa ggccttatct tcctttgttt cctctgggag caattccttc
     8281 gctggtaggt ttggtgttga atagccgaca tactccgcat tttggagagc attttcaggt
     8341 ttcaacataa agttgataaa ggcataggct gagttttggt ttttaactgt tttgggaatg
     8401 accatattgt caaaccaaag attgctggcc tctgtcggta ccacataacg tagattttca
     8461 tttttttcta acatttggct ggcttcacca gagaaggtca cgccgattgc aacattattc
     8521 tgaatcatat agcccttcat ctcgtccgca acgatagcct tgatatttgg agtcagtttg
     8581 tagagcttat ccactgtctc ttccaactgc tgcagatcct tggagttgag gctgtagccg
     8641 agggaattga gtcctagtcc cagcacctca cgcgccccat caaagagcat gatagaattc
     8701 ttatactccg gcttccaaag gtcatcccaa tgctcaggcg cttcatctac catggtttcg
     8761 ttgtagacaa ttcctaaggt tccccagaag taagggatgg agaatttatt acctgggtca
     8821 aaggactggt tgagaaactc tggtccgata ttttcgattc cttcaatttt tgaataatca
     8881 agcggaacca agaggtcttc gtccttcatc ttgttaatca tgtattcact tggaatggca
     8941 atatcgtagg tcgttccacc ctgctttatc ttagtgtaca tggcttcgtt ggagtcaaaa
     9001 gtctcgtact gaacttgaat tcctgtttct tctgtaaact gagtcaagag ttcaggatcg
     9061 atatagtctc cccagttata gataaccaat ttttgactat ctcgactatt gattttacta
     9121 tctaaatgag tcgcaattcc ccacaagaca aggataatcg ctgcaattcc tgctaaaaat
     9181 gaatagattt ttttcatgct tgctcctcct tctcacgaga gataaagtaa taacctacaa
     9241 ctaggataat actaaagaga aagactagag cagacagggc attgatttct aaggaaatcc
     9301 ccttgcgagc acgagagtaa atctcgactg atagggttga aaagccattt cctgttacaa
     9361 agaaggtcac ggcaaagtca tctaacgaat aggtgaaggc catgaaataa ccagtaatga
     9421 tagacggagt caggtaagga agcatgattt ccttgaacat ctgaaattga ctagctccca
     9481 agtcataggc cgcatgaatc atgtcgccat tcatttcctt gagtcgaggc aagaccatca
     9541 agaccacgat aggaatggag aaggccacgt gactagatag aacggtcaaa aagccaagtg
     9601 aaaacttgag ttgggtaaag agaatcaaga agctagcacc aatcataacg tcaggcgcaa
     9661 ccatgaggat attattgagt gatagaaagg cttcttggta tttcttacga gactggtaga
     9721 tgtaaatggc accaaaagtc ccgataatgg tcgctatcaa ggctgatagg aaggccaaga
     9781 aaaatgtctg agccaaaatc agcatgagtc tcccatctcc aaacatggtt tcaaagtgag
     9841 tccagctaaa acctgtaaag ctattcatat catcaccagc attaaaggca tagccaatca
     9901 agtaaaaaat cggcaggtag aggaccagaa agaccagtcc cagataaagg ttggcaaatt
     9961 ttttcatcgt tctctccttt ccttagtcac ccacatggtg atgaacatgg tcaggatgag
    10021 aatcacaccg atagttgaac ccataccata gttgtcattg gttagaaaat tctgctcaat
    10081 agccgtcccc aaggtgataa cgcggttccc accaatcaaa cgggtcagca tgaagagact
    10141 caaacttggg ataaagaccg actgaacccc acttcgcaca ccgttcatag atagagggaa
    10201 gatgacatga cggaaggtct cccacttagt tgcaccaagg tcgtaactag cattgatgag
    10261 attattatcc atttcgtcta agacattgaa aatcggcaaa atcataaagg ggagctcgat
    10321 gtagcttgcg acaaagataa atgagaaatc agtaaatagc aactgctgtg aaccaattcc
    10381 gataaattcc aagaattggt taatagagcc attctgacca aaaatcccga taaaggcata
    10441 agccttaaga agaaggttga tccaagttgg cagaataatc agcataatcc agagttgacg
    10501 gtgtttgaga cgggtcaaaa agagggccgt cggataactg ataagcagtg tcacaaaggt
    10561 cacaatgcct gcataaagca ctgagttgaa actcatttta agataggtca agttttgtga
    10621 cgcaaagtaa gatttgtaat tttctaaact gaactggcct tcgatgttga aaaaggattg
    10681 accgaaaatc aagaccaagg gtgccaatac aaagagcgca atccaaagca tgtagggtac
    10741 tacaaagagt ttagagcttg ttttcttcat ctctttcctc ctcgattgca ttgatcaaac
    10801 ctgcttcttg ctcttcgatt tctacgtact cctcaatacg agcatcgaac tcttcttcgg
    10861 tttcattgag acgcatgatg tggatgtctt ctggttcaaa gtccagaccg atttcctcac
    10921 ccacgatagc cttacgggtt gagtggatca tccattcatt tccaagttcg tcataggcga
    10981 taatttcata atgaactcca cggaagagct gggtgtcgac cttaacttgg agtttgcctt
    11041 cttcaggaag ggtaatgcgc aagtcctctg gacgaataac gacctcaaca ggttcatttg
    11101 gcttcatccc accatcaacc gcttcaaagc gtttgccgtt aaattcgacc aagtagtcct
    11161 caatcatggt acctggcaag atgtttgact ccccgataaa ggtggcaaca aagtggttga
    11221 ttggctcatc gtagatgtcc acaggggttc cagactggac aatctcgcca tcattcataa
    11281 cgaaaatcca gtcactcatg gcaagagctt cttcctgatc gtgagtgaca aagacaaagg
    11341 taatgcccaa tcgttgttgt aattcacgca attcgtactg catgtctgtt ctcaatttca
    11401 agtccagcgc tgataaaggc tcgtccaaca agaccacacg gggttggttg atgatagcac
    11461 gggcgatggc cacacgctga cgttgtcctc cagaaagttt gcggatggaa cgtttttcat
    11521 aaccttccaa ctgaaccatc ttgagaactt ccgctacacg ctgctcgatt tctttcttat
    11581 caattttacg caagcgaagt ggaaaggcaa cattttcaaa cacattcata tgtgggaaca
    11641 aggcatagga ttggaagacg gtatgtacgt cgcgcttgtt ggttggaata tcattgatac
    11701 gaacaccgtc tagcatgata tctcctgtcg tcgcatccag taaacctgca ataatgttta
    11761 ggatagttga tttccccgaa ccagatgcac ctagaagggt gtagaatttc ccttcttcca
    11821 actcaaagtt gatgtctttg agaaccttgg tgttgctgtc ttcaaaaact ttagagacgt
    11881 ttttgaattc gataattggt tttttcaatt ggcataaatt ccttcttttt catagattaa
    11941 cagatcgggg ctctgtcagg tcgccactac ctcttgcagg gagtaaaacc acctgcatac
    12001 atcttcgcta ccaataggct ttcaccccct ttccatgaca tagcagcagc ttttcaaata
    12061 cagcttccta cttgctttca cccaagatcc ggacttctct ttcaagcgta atacctgagt
    12121 gttccttgac tttttcgata accgattgga tcaagtcctc gtagtctttg gccgttccat
    12181 ctgcgacatt gatcataaat cctgcatgct tttctgacac ttctacgcca ccgatacgat
    12241 agcctttcaa gccagcttct gaaattaact gacctgcaaa atgcccgact ggacgcttaa
    12301 agaccgaacc acaagatggg tattccaaag gttgcttgag ttcacgtagg tgcgtcaagc
    12361 ggtccatttc ctgcttgata acctgatggg ttcctggagc tagggcaaat ttaactgaca
    12421 agacaactgc accagactcc tgaatagctg aatggcggta accaaaagcc aagtctttag
    12481 cagacagggt ttcgatttct ccattcttgg tcaagacctt acaagactgc aagatatgag
    12541 caatctcgcc accataggca cccgcattca taaagacagc accgccaacg cttcctggaa
    12601 taccacaagc aaactcaaag ccagttaaac tatgacggag ggcaatgcga gttgtttcaa
    12661 tcaagttagc cccagcttct gcttcaatgg tatagccatc aacagaaacg ttattgagct
    12721 tgtcacacaa gatgacaaat ccacgaatcc caccatcacg aacgatgata ttgcttgcat
    12781 tgccaagaac catccaaggg atattttctt ggttggcaaa tttcacaacg cgagccaact
    12841 caaaacgatt tcgtggaaag accaaataat cagcctctcc acctactttt gtataactat
    12901 agctatgcaa gggttcctta aaacggatat caattccttc taagatttca agcatttttt
    12961 ctcttacaga catgtcactc ttccttttac aaaattcatt ccattatacc atttttagag
    13021 acatttgacg accataaaaa taccttgttt ggattttgca taagaaaaag aggttccccc
    13081 ctttttatga ttttttacaa aagatttcct tggttccata ggcgaccaga acgagctcca
    13141 gtgctagaat cacttcaacc aagactggat ttgtcaacca gcctacttgg aaaagagatg
    13201 gtgccagatc aaagaaggca tgcaagccat aggctgctag gagataaatc catttcttct
    13261 ggcgaacagc ttggtaaacc caaactgtca aaagtaattg gaaaccaagc gccaagattc
    13321 gctcaaaacc aagcaaataa atctgccaga ccgaaagtga ctgaatggtt tttaacatat
    13381 tttcagacag taattgcata acctgtggat tctgagtttg aactgccgaa agaacgatgt
    13441 aaagattgag taaactagta aggcctagaa aaatcaactc caagccacca tgccctaggc
    13501 cataggccaa ggcatctgcc ttttccaaac ttctcttttt ctccaaccat ttgaagaaaa
    13561 caagacgagc cgtttcctca aaaaatgctg ccatggctag gccatagata atatagataa
    13621 gcggatgatc ttgcaagagg gcaatactac cgtctttttg agggtgcaaa atcaagatat
    13681 gcaccagttt ttccaaaatc tgcgaagaga caaagaaagc gactgcaccc aagcccaaga
    13741 cagctagatt aatctggtat ttctttttgg caaaccaaat gcttcctatc agaaaagcta
    13801 gcaacagcaa catggtaata atgatatgaa tggtcatttt cttctcctat tctgcctttt
    13861 caatatcttt tttcatctcg tcaacattga acttagcaaa caagtattga cggtcttgga
    13921 ctgggaaacg ttggtccaac tggtcaactg ctcccacctc gataatgcct tccttgatga
    13981 caaaatccat gacatgtcca ccgaaggtca aatcatctga tatgaagtgc agatggtagc
    14041 ctgccacact gaccccatgg aaaatctcag gcgtccagaa accaacaata gtccccgcaa
    14101 cattgtcacg actatattcc ggttgatggg ttgcgacatc agcaaacttg gtatcgggtg
    14161 ttgacttagg aatcatacgc acatgcatat gtaaaaattc cccccgaatc ttgatagagc
    14221 ggaaaagatt ttccccatca taataagact caattcgttc tttcaattcc ttgtctgtca
    14281 tctcaaagcg ctggcggaaa atgacctctg cccgatgcgg taccactgca gcgtaaggaa
    14341 taagggcatc tggtgacact tccacaattt ctggctggtc tcctgaccct ttggcctgat
    14401 aagccttgcc atccaagaca atcaattccc catcaatgga atccaaggtt cccaaaccaa
    14461 ggtcaccatg ttctagcaat tctcctactg tcatagtacc accataaagg ccagccatca
    14521 aggctccaag agtattgtat tgaaataatt tcactggttc ctgcacgttc ttatccattc
    14581 actttcttgt ctgttattct ataaatctta ctcctaagta taccacattt gcccctagat
    14641 gtgaacgaga gaaacgctct agacattgcc aagaaggaaa aaaaagggta caatgtaaca
    14701 aaatcaaggg aggtctggaa tgaagaaaca aagcaagtac aaagaggtcg tttcctatct
    14761 gaaaaatggt atcgagtctg gacgatttcc gacgggtagt cgcctgcctt ctatccgtca
    14821 actgagcctt gactttcact gcagcaagga caccattcaa cgagccctgc tggaattacg
    14881 gcacgaacaa tacctctatg ccaagcctca gagtggctac tatgtattag aacaagggca
    14941 acatcaagac ctagaaatcg aggttaccga cgaacatgcc agtgcctatg acgatttccg
    15001 actctgtgtc aatgaaacct tgattggccg agaaaactac ctcttcaact actatgacaa
    15061 tcaagaagga ttagaagacc taagacagtc cattcacaaa ctcctctttg agcaagctct
    15121 ctactgcaag gctaaccaac tagtactgac ttctggaacc caacaagcct tgtttatcct
    15181 ctctcaaata tcctttccta gacaagccaa ggaaatcttg gtggaacagc caacctacca
    15241 tcggatgaat cgcctcttga ttgcacaggg gctggactat caaacgattg aacgaggcat
    15301 tgatgggatt gacttggagg agctggaagg ccacttcaaa acaggaaaaa ttaagttttt
    15361 ctacaccatt ccccgatttc actatcccct gggacattcc tattctgagc aagacaaacg
    15421 atctattctt aacttagctg ccaagtatga tgtctatatc gtagaggacg attatctggg
    15481 tgatttggac tccaagaagg gccaaacctt ccactatctt gatacagagg agcgtgtcat
    15541 ttatatcaag tccttctcga ccagcctttt tcctgccctt cgtattacag cactcattct
    15601 tccaaatgct atcaaagaag catttgtggc ctacaaaaat atcctagact acgacagcaa
    15661 cctcattatg caaaaggccc tgtcactcta tattgacagt caattgtttg aaaaaaatcg
    15721 tttggctcgc ttgaccaatc atgaatctta ccaaaaacaa atcgaggaaa ggataactaa
    15781 aacaccttgt ccccttcctc attattccct acacgatggt ttattgctag acctgagaca
    15841 gtatcctaaa atcgccagtc tcaaacacag tcaactgggc ttggacttct ttgaagaggc
    15901 ctatttaagc acctgtcctt atcaatttgc caaggtgtcc ctagacaatc tggaaaatgt
    15961 tttaaactat ttaaaagcag aattggaatg acatccaact ctgctttttc ttattcttca
    16021 acttcagtta gacttgctgc tttttcaaga tagctttggt aagttccttc atccttaagt
    16081 ttttggacga ccttgtctac cacttctttc aaatcatcac tattttttct aagagcaacg
    16141 gcattggcgt cgccgtcctt catcttcaag ctgacagttg cgacagctaa gccagcgttt
    16201 ttagcagcat aactaagtgc aacaggctca tccatatgaa cagcatctat ttttccagcc
    16261 tgcaattcat tgactgcttc acccatatta gttagggaag ttaattgaac ttttggcaat
    16321 tgttccttga ccattgattc tggaacagtc cctttttggg ctgcaatatt agcactttct
    16381 aggctagtta aatccttgta tttttccaca tcagccttac gaaccaagaa actaatcttg
    16441 ttttcatagt atgggattga aaaatcaaag acttcttttc tctcgtcagt agcactaatt
    16501 cctgcaactg ctaggtcagc cttaccagtt tgaagactgg tcaaaacatt gtcaaaactc
    16561 atgcttgaga tttccaactt aaccccaagt tcatcagcga tagcctgagc catgtcgatg
    16621 tctgcaccga ctacctggtt ctttccatca accaatgatt gaaattcaaa gggtgcatag
    16681 tcaggactgg ttgccacaac taatttccct ttttgtttga tagcatccac agcagactga
    16741 gaaccattag aacctgactg acaagctact aagaagaaac ttgcaagaaa attacataaa
    16801 acaaatatcc atttttttga tttcataaat aaacaacctc tctgttaatt ttgtataatt
    16861 ataacgatat ccaagttact tgtcaagtgt tttttaaatt tttatctcaa aaatattttt
    16921 tcgttcaaaa aaaggagcca tcagttgatt tcaagctccc ttttatacag aattaaacta
    16981 ttttatagtt cgacaatctt acctgtttca aagtagacaa cccattcaca gatattttta
    17041 gcatagtcac cgatacgctc caagtaggaa ataacttgga aataatcacg acccgtaaca
    17101 atggcttctg gatttttctt aatctcttca gtcgcaaggt cacggatagt ttcaaaatag
    17161 tggttaattt gctcatccat ggaggccacc cggtatgcgt cgtcaacaga accattaaga
    17221 taaagatcaa gtgctgcttc cacaacgctt ttaacttcac gtcccatttt tttaatttct
    17281 tcctctacag ctggaatgcg ctcttccccc ttcatacgga tggttgcctg ggcaatggct
    17341 acagcgtgat cccccatacg ctccacatct gatacagcct taaggacagt caagactgta
    17401 cgcaaatctt gagagactgg ttgttggagt gcgatcattt caaatgattt cttttccagt
    17461 ttcacttcgt attcatttac ttctgcatca tcttcgatga cctcttttgc caggtcacgg
    17521 tcatgcgtga caaaagcacg taccgtacga ttgatttgtg agagcacttc ttgtcccata
    17581 gcgtagaact ggttatgtaa tttctctaaa tcttcttcaa attgagatcg taacatcttt
    17641 catctcctta tccaaatttt cctgtaatat agtcttccgt ttccttgtgt tggggatcaa
    17701 ggaacatctg cttggtatca ttaaattcaa tcaaatctcc atctaggaaa aatcctgtct
    17761 tatcagagat acgtgaagct tgctgcatgg aacgggttac cagaagcatg gtgtacttgt
    17821 cttttagacc atacaaggtt tcctcaattt taccagctga aatcggatcc aaagccgaag
    17881 ttggctcatc caagaggatg attttaggac tagttgccaa gacacgggcc acgcagacac
    17941 gctgctgttg accacctgac aatccaatag ctgaatcata tagacgatcc ttgacctcat
    18001 cccagataga ggcaccttgc aaggcttttt ctacggcttc atccagaacc tgcttatcct
    18061 taattccatt gatacgaagc ccgtagacaa cattctcata gatagtcata gggaaaggat
    18121 taggttgttg gaaaaccatt ccgatttcct tacgtaattc aaccgtatct gtacgcggac
    18181 tgtagatgtt gtgaccattg tacaccacgg atccagttgt ggtcacctct ggattgagat
    18241 ctcccatgcg gttgagagac ttgaggaggg ttgacttccc tgatccagat ggaccaatca
    18301 aggctgtaat ttccttaggt tggaaagata gggaaacact attcaaagcc ttctttttat
    18361 tataataaac ggacaggtct gatacctgta aaatcgcatc tgtcatacgg tttcctttct
    18421 aaccaaagtg accagataca tagtcattgg tggactgtag cttggcattt tggaaaatag
    18481 ttgcagtctt gtcatactca atcaaatcac ccaagtaaaa gaagcctgta tagtcacttg
    18541 cacgagcagc ctgctgcata ttatgcgtta caatgatgat ggtaaagttt ttcttgagct
    18601 caaacatggt ctcttctagt tgcatggtcg caatcggatc caaggctgag gctggctcat
    18661 ccattaagag gatatctggc ttaacagaga tggcacgagc gatacagaga cgttgttgct
    18721 gaccacctga taaggtcaag gctgacttgt ggagatcgtc tttaacctga tcccagaggg
    18781 cagcctgacg aagggaggtt tctacgattt catctaggac ttgcttatcc ttaactccag
    18841 cacgttcatg cgcaaaggta atattacggt aaattgactt agcaaatgga ttgggacgtt
    18901 gaaaaaccat tccaatgtgt ttacgcattt cataaacgtt gatttctgga cggttgacat
    18961 caattccacg atagagaatc tgcccagtta ctttagcaat atcaatagta tcattcatgc
    19021 gattgagact gcgtaagtag gtagatttcc ccgatcccga cgggccaatc aaagctgtaa
    19081 ttttatttct ttcaaattgc atatcaatcc ccttaatgga ttcattttta ccatagtaaa
    19141 catggacatc cttagtagaa agggctactt tttcttcagg aaaggtaagg atatgcttct
    19201 catcccagtt atatgttgac atggcttctc ctttaggcag cggttaattt cttgtgtaga
    19261 tagcttccga acttacgagc tccaaagtta aaaatcagga taaagatcag gagcacagcg
    19321 gcagaacctg ctgatacaat ggttccatct ggaatagtgc cttcactatt gactttccag
    19381 atatggacag ccaaggtttc tgcttgacgg aagatagaga tggggctagt cacactgagg
    19441 atattccagt tagaccagtc aagagctggc gccgattgcc ctgctgtata gatcagagct
    19501 gcagcttcgc caaagatacg accagatgcc aagacgacac ccgttacaat acctggaagc
    19561 gcttccggaa taacaacatg aaccactgtc tcccagcgag aaatcccaag agccagacca
    19621 gcctcacgtt gggtatggtg aacgtgtttc aaactatcct ctacattacg cgtcatctga
    19681 ggcaagttaa agactgtcaa ggccaaggca cctgaaatga ttgaaaatcc atactcaaac
    19741 tggactacaa agatcaagta accaaagaga cccaccacca ctgatggtaa agaggacaaa
    19801 atttcaatac aagtccgcac aaagttggta acaggacctt ttttagcata ttcagccaag
    19861 taaatcccag ctcccataga aagaggtaca gaaataatca aggtaatgac caataggaaa
    19921 aaggaattgt aaagctgaat gccaatccca ccacctgctt gaaaagcaga agaccttcca
    19981 gtcaagaaag accaagagat atggggcaag ccccgaacca agatatagag aatcaaggaa
    20041 gccaagattg tcacaatgat gctagcaatc gtatagagga cagctgttgc aagtttatct
    20101 aatttcttag cgcgcataat ttttctttcc tctttctttc gtaatcaatt taatcacact
    20161 gttaaaaact aagctcatca agagcagtac caaggccagt gaccagagaa cattattatt
    20221 tacagttccc atgacagtgt tcccaattcc catagttaat atagaagtta aagttgcagc
    20281 tggtgtggtc aaggaagttg ggataacagc tgagtttccg acaaccatct ggatagctag
    20341 agcctcacca aaggcacgcg ccatcccaaa gaccactgca gtgaaaatac cagaacgggc
    20401 cgccttcaag atcacacgcc agatagtctg ccagcgagtg gctcccatag cgaaactggc
    20461 ttcacgataa taacgaggaa ccgcacgcaa gctatccgtt gtcataaagg ttacggtcgg
    20521 caaaatcatg acaaagagga cggaaatccc tgacaaaatc ccaaaaccag tcccaccaaa
    20581 gacactgcga acaaagggaa cgacgacttg caagccaata aatccgtaca ctactgaagg
    20641 aatcccaacc aggagttcaa tagctggttg caaaatcttc gccccttttg gtgatacttc
    20701 ggtcataaaa actgctgcac caatagcaaa gggtgttgcg ataagggctg agagaatggt
    20761 aacgataaag gaacccaaaa tcataggaag ggcaccaaat tctttactag aaggattcca
    20821 agttcctccc aaaagaaagt caaagatatt cacaccattg acaaagaagg tcgacaagcc
    20881 tttttgcgct acgaaaacca aaatcatggc cacaaggatg actatcaaag aaagacaggc
    20941 aaaggtcaaa ccttttccta atttctccag acgagaattc tttgatggaa gcaacatttt
    21001 cttagctaat tcttcttgat tcattattgt ctcccttcca acactgtcac agttccggca
    21061 gcatcttttt caaccttcat ttccttaatc ggaatatact tcaatccttt gacaatccct
    21121 tcttgggtct catccgagag aacaaaattg agaaattctg cagccaactc attgggctgc
    21181 cccaatgtat acatatgctc ataagaccac aagggccaat tattgctact tatattttct
    21241 ggacttaagt catagccatt caacttcatg cttttgaccg aatcatctat ataggtaaga
    21301 gataaataag agatagctcc tggacttttt gatacgattg attttaccgc tccatttgaa
    21361 tcctgctcct gactttgcat ggcagactga ccttccataa tgacagtatc aaaggtagca
    21421 cgagagccag agccggctgc ccgattgata acagagatgg gtaagtcctt accaccaacc
    21481 tctttccaat tggttacctc acctatgaag atttgacgaa gttgctctgt cgttaggtta
    21541 tcaacatcaa cctccttatt gacaatcaga gccaagccag ctaccgcgac cttgtggtca
    21601 acaagagcag aagcatcaat tccgtctttt tcctcagcaa atacatctga gtttcctata
    21661 tcaactgccc cagactgaac ctgggacaag cctgtaccag aacctccccc ttggacattg
    21721 accgtttttc caacatggat cgtgccaaat tcatctgccg ctacttcaac caagggttgc
    21781 aaggcagttg agccaacagc cgttatggat tctccacgat caatccagct agcacagcct
    21841 actaaacaag ccgtcagcca aaaagcgata agagacagag caagcttttt tctttttttc
    21901 actgtttttc tcctcgaaaa taattatgaa tactgtgaat tttttaagta gttctttatg
    21961 agttgacgca tgaattctta ccaaatttct gcgcaattga ttatttatat aatataggct
    22021 atattactct ttcctaacct ccttttttca tatgtggata aaatctcttg tctatccctt
    22081 cccccattgt cacccattat agtcatttcg tgtctctttt tccccttttt aatgcaaggg
    22141 aaattactct ccttagatga taatccaaaa gctagaaagg tatctcaaac ctctctactc
    22201 tcccagacta gtttacaact aaaaggaaaa gattctattt tatgagaaat ctagtttaca
    22261 agcggtaaga acgctaataa ctaaacttct tgtactcttt gaaaatctct tcaaaccagt
    22321 gttttgagct atctatggct agcttcctag tttgctcttt gattttcatt gagtagtaaa
    22381 actacatgta atggcaatca agatatcaag aatcatccta ctaaaaaaat ccatactttc
    22441 actataacat agaataagat atttgactag cattttcatt tgaatctgag gccttttgga
    22501 aaataatttt tcaaaacatt tccagtaacc tttgcaaagc ccaagccatt gcctttaacc
    22561 aaaacttggt accaaccatt tggcagactt tctgccagct gaacggtttc tccagccgca
    22621 tacttgacaa acgcttcttg gccaatttca accgactgtt cgacctgact cggtttcaag
    22681 gctaaaccaa gagcgaaact gggctcaaag cgtttcttct taaaagtacc cagatgcagt
    22741 ccattgcgag caatcttgag cttccctaaa tctggcaaaa gttctggcaa gagataaagc
    22801 tggtctccaa aaatctgcaa gatacccggt agattgacct tcaaatggtt ttgggcaaat
    22861 tcctgccaca aggcaacttg ttcacggctg aggttactct tacttgcctt aaatttagga
    22921 gctggattgt tacccttaaa ctgtagatgg gcaacaaact gaccctctcc cttaaactga
    22981 tgagggtaca tccgagccgt ttctggcagg tcaataccag ctaccattcc attgatatgc
    23041 tctactggca acaagtcaaa atcatactct tccagcaacc aattgacaat ctcttcgttt
    23101 tcctcgggtg cccaggtaca ggtcgaataa accagacgac caccttcagc taacatggtc
    23161 actgcatcct ccagaatttc tctttgcaag ctagcacatt gactcggata atctaagctc
    23221 caatagtcca tagcatcagg ttgcttacga aacattcctt caccagagca aggggcatca
    23281 agaacgatta agtcaaaata gcctttaaag accttgacca agcggtcggc agattcattg
    23341 gtcaccacga catttgtcgc tccaaaacgc tccatgtttt caaccaaaat cttagcccgt
    23401 ttgcttgaaa tttcattgga aacaagtagc ccctcccctg ctagataggc tgccagttga
    23461 gttgatttgc cccccggtgc agcagccaag tccaagacct tcataccagg actgggttgg
    23521 gctacttgag ccaccatttg agcagcaggt tcttgcgaat aaactaaacc tgtagcatgc
    23581 tcaggcgatt tccctgaaac cttcccatag tggccccaag gggtttgagt aatggcatca
    23641 gaaaaggaaa gttgctcttc ttttaaggga ttgacccgaa aggccgaaac cgcttcctcc
    23701 tcaaaagagg caagaaaatc tcttgcctca tctcctagta tctctttata tttttcaaca
    23761 aatccttctg gaaattgcat ttaagttctt ttcctttcgt aaatatagga ctgaatttcc
    23821 tcctgcatct caagaggcac catcatgacc ggctgtctgg tttgaaaatc aggagcttca
    23881 ccaaaaaggg tcacaacccg atagcccaga ctttccccta aaatactagc tgcggcataa
    23941 tcccatggtt gcagataagt gagataggtc aacaaacgcc ctgacaaaat cttggcaaaa
    24001 ctaatggccg cacttccata gacacgaaca ccaagaaccg atcggctcaa atcagccagc
    24061 ccccattcat tggtttccag cataccacta ttccctgcaa tgagaaaatc tccaagtggt
    24121 ttagttttaa aaggagctag ggacctatca tttagacaaa ctggaaattc cccaccaccg
    24181 tggtaacaat cccctttgac cacatcataa atcagaccaa actgtccctg accattttca
    24241 aaataagcca tcataacagc aaaatcttcc tgctgggcta caaaattatt ggtaccatca
    24301 atgggatcaa tgacccaaac cttgccctct tgaaccgagg ctcgcagaca accttcttca
    24361 gcacaaatct tatcctcagg ataacgggac aaaatctcac caaccaagag ttcctgaact
    24421 tctttgtcca gtctggtcac caaatctgtt ggagaggact tggtttcaac acgcaagtct
    24481 tcctgcatat ggtcaagaat gtactgacct gctttcttaa caagctcttt agcaaattca
    24541 aatttacttt ccaagagaaa tctttccttc ccctttttct ttggcagcct gaactgctcg
    24601 gtaaagtgaa tagccactaa ccgtttcaaa ttctcgtccc aaacgtttct cttcgctctt
    24661 gcttggcacg accgctttga atcccttata ggagtccagt aactttttag cctctacttt
    24721 gccttcatag gcagcttcaa catcattaaa aaaagaaagc actgaagcaa gttcttcagt
    24781 gctccacgac aaatctagtg ggtaactata ctgtttgttc attaactaat accagctctc
    24841 attcttgctt cttttagttc ttgcttacga taactacgag ggagaaaagc acgaatctca
    24901 tcttcattaa aaccgatttg catacgcttg gcatcaataa taattggacg acgcaaaaga
    24961 ctaggatact gctcaatcaa atgaagcaat tccgataccg aaatactctc tacatcaata
    25021 ttcaattttt gaaaaatttt tgaacgagtt gaaatgatgt catcagtacc attttcggtc
    25081 aaggaaagga tgtgttgcaa ttcttttctt gttaaaggac tggtcataat attgtgttcc
    25141 acaaagggaa ccttatgttt ttctaaccag gccttagcct tacgacatga tgtacagctc
    25201 ggtgatagaa atagtgtaat catgcttttc tcttcttatc tatactttgc tacttctatt
    25261 atacaaaaaa ataaagcgct tgactaggga tttttagaaa aaaagcctat tttttcaaga
    25321 aaaataggct gtttgcgaac gattgacaca attggatttg gttaattcac tcttaacgat
    25381 ggttttaaac gatatatatt tttatatatg caaattaaaa acatctttca tttcacttcc
    25441 tacgactttt cagacacaga tagccaaaga agctttcata gagggcaaaa aagaggagga
    25501 aggcatgaag aaagaaggtc tctggcaaaa tcataataac aggatccttg gctggatcaa
    25561 aaagccaggt atcatctccc acaaagagaa tttgatggaa aagagtaaag aattggtcaa
    25621 aaccaatcaa aactccccca agtccaatca tcacaggtaa gactactaga gtcaggagac
    25681 tttttcgata aagagacaaa aagtcctttt tcacaatcct attgacaaag acatagaaac
    25741 ttggcagtgt cactagagct actagctgaa ccaaatgaaa gagattcttg accactgcga
    25801 aatggtgcag accagctgct gacgaacgaa aatcaggcat ctgtaagacc tgactaaaag
    25861 gattggtcag ataattcatc aagatatgaa aattgtattg aatggtttct ggttttagat
    25921 agactcgatt cgttaagttt agccactgaa tctccatagg atagaaaatc caagccagat
    25981 aaatggtcag aaggattgag agggagagga gaaagagcat agagccccaa aaggtcaatt
    26041 tagttttcat caaaatccca ctccgcaagg ctagaaacca catgtgtcgg tgcgattggc
    26101 aggccagcta cttcttctgc cttggtaaaa cctgtcgtca ccaagagtgt tggaatgcca
    26161 ttgtcaatcc cagcccggat atcagtcaaa taattgtccc caaccatgat taactcttca
    26221 cgttccaaac ctaagtgctc aaccgccttg tccataatga tggcatttgg ttttccgata
    26281 taaaccggct tcactcgtgt cgctacttca agcagcgtaa tcagtgagcc agcacctggc
    26341 aaaagaccac gttccgtcgg gatgttgagg tcaggattgg ttccgataaa atgggcaccc
    26401 ttttgaatag caagagttgc tgtggcaaat ttttcatagt cgacttgcca atccagacca
    26461 actaccacgt aggcagggtt ttccttgtct tccacataac cagccgcctt gatggcttcc
    26521 ttgagtcctg cttctccgac gacatagacg gtcttttcaa gccccaaatc attcatatag
    26581 tcgatggttg ccaaagtcgc tgtgtagaca gtcgatagag gcgtatcgat attaaaattc
    26641 tgagccaaca tctccttaac actctctgga gtgcgggttg tattgttggt tacaaagaga
    26701 tagggaatgt cccgcttttg caattcatga acaaaagtct ctccagcagg gattcggtct
    26761 ttccccttat aaatggttcc gtctaaatca attaaatagc ctttatattt catctatttc
    26821 tccctaaacc ttttttattt cttgccaagt aatgattgct tgggcattga taaccccatc
    26881 acttgtaatt tcatgcttgc tttccagtcc agtccgttca acagccgatg taatcacccc
    26941 acctggtcga acttccttga catacttgag gttgattttc ttgggaatat agtgggtcaa
    27001 aaaatccgct cccatgacct caaaaatcca gtccaagtat ttactgttat tgacatgacc
    27061 attcatatcc aagtcgtaaa aacgaacatg gtaatccttg ctgatcggtt cttccaagga
    27121 ctcatacttc ggtccacgga taagtttttt atcaaaatca gactggtaag gagccacaat
    27181 ctcaggttca acagcatgga cttttcgact gtcgcggtcc atgagaacaa aggtcgccat
    27241 catatggatg agctcctgcc ccgcttcatt ataaatagta aagcgacggt agcaaaaaag
    27301 tcgattgtag ctcaaggctt ccgtttcgat ggtaatttct tccgcaaaac gaggcaaacg
    27361 aaccacctca atatcatatt ctgcgataat ccagaccaga ttatagtctt ccaaaatggc
    27421 cttatcacta actcccagtt caatcgattg catccctgaa acttgcagtg acagcaaaat
    27481 cacatctgga agtttgatat gaccgttcat atcagccata tcaaaaggaa ttttcatttt
    27541 catttgataa gttaagccca tgatcctact ccaaaataaa tcgttctgct acagtatctc
    27601 ccaaaaagag acctctcttt gtcatgcgaa cgtggtcacc ctcaatctgc atgaggcctt
    27661 gttgaaccaa atctctgaca atttctccat aaagtccagc aaaagactgt ccaaattttt
    27721 cctcaaatcg cgccatggaa accccggatt tcttgcggag tcccaagaac atttcttctt
    27781 ctatttgctc cttttgactc aggtgatctt ctgtaataca agcattgcct tcctcaaccg
    27841 cactgagata atgacgaatg ggaccatgat ttttatagcg tactccattg acataaccag
    27901 atgcccctgc accaatacca tagtattcag cattgtccca gtacatgaga ttgtgacgac
    27961 tttcaaaacc gggtttggag aaattagaaa tctcataatg ctcaaaaccc gctcgctcca
    28021 gctctgcaat gatgtactca aacatctccg cttctagttc ctccttaggc agaggcaatt
    28081 tcccacgtcg catccggttc ataaagaccg tatggttttc taaaatcaaa ctatacaaac
    28141 tcatgtgggg aatatccaat ccaatggctt tagccatatt ttcctttact tgctccatgg
    28201 tctgaccagg cagagcataa atcaaatcaa tggagatgtt gtcaaaacca gccagtttca
    28261 ggcgatcgat attttcataa atatccttct ccaaatgact gcgcccaatc tttttcaaca
    28321 tcttatcatc aaaggtctgg acacctagcg aaacacgatt gacagccgaa tttttcaaaa
    28381 cagctatctt atccgcatcc aaatcgcctg gattggcttc aatggtcaac tcttccaaga
    28441 cagacaaatc caagttttta gtcaagccat tcagtaacac ctccagttgc ggagccgaca
    28501 gggctgtcgg tgttccacca ccgatataaa gggttgacaa cttttcaata tcataagaac
    28561 gaaactcttc cagcagatgc tctaaatagc tgtcgactgg ctgatttttg atgaagacct
    28621 ttgaaaaatc acaataataa caaatctggg tacaaaatgg gatgtgcaca taggctgacg
    28681 ttggtttttt ctgcatagta attattatac cacaaagact agattccaga taaaaatcac
    28741 catccccaga tacatagtcc gtccggagat ggtgatggtt tattcttctg ttatatcaat
    28801 cacaatctct tctgagtcat caagagcttc ggctttttct tgccattgtt ccttgagatt
    28861 atttaattga ttttttgatg cttctgtcgc ttgaaaagca taggatttag cttgagcaag
    28921 tatactgtcc acagtgattt cacctgactc aacctgttct tttgttttca gaacaaaatc
    28981 tgtagcctgc tccttaactt ctgtcagttt ttcacagact tgctccttgg catactccgg
    29041 atcttctctc aaatcatcta gaaaatcttg agcctgactg caaacttgtt tgcccttatc
    29101 acttgttaaa aacaaggcaa gagctgcacc tgaaacggtt cctaaaagga ttgaggataa
    29161 tttacccata aggattctcc ttttttattt tttgaaaaat ttacttgcaa gacgaagagc
    29221 tgacagactt gcaccagtct tgagtgtttt tgaaccagct gatgaagctt tcttgctcaa
    29281 gacacgcgca tggtcattga ggtctgaaac agatagagat aaatctgcaa cagcactgaa
    29341 gagtggatca atcgtagcca ccttgacatt gatatcatct gccaagacat tgaccttagc
    29401 caacaactca ttggtgtgat gcaaggtcac atccacatct gaagtcaagg ttttaatcgt
    29461 cttttctgtt tcatcgatga cacgaccaag cttttgtaca gtaatgatca gatagaccaa
    29521 aaagacaatc acagctaggg caacaagaat atatgcaact tctaacattt agttttcctc
    29581 ctctgtaata tagtaagggg ccttctttcg attttgataa ataacgatca ttataccgag
    29641 accgataaag acaactgaca gccattggga cactcgaagg ccgaagaaca tgagactatc
    29701 tgttcgcata ccttcgatga ccatacgacc gaaaccatac caaatcaagt aaaaggccgt
    29761 gatatgacct cgtctgagac tcttccattt ccgtctaaaa atcagaatca aggcaaagcc
    29821 aagcagattc catagagact cataaaggaa agtcggttga tggtagctcc cctcaatata
    29881 catctggtca cggataaagc caggtagata atccagatta tccactgttg caccataagc
    29941 ttcttggtta aagaaattac cccaacgccc caaactttga gcaatcataa cgctaggcgc
    30001 cgcaatatct aaaaaatccc aagtattgat gagtttacgg tcagcaaaga tatagagcac
    30061 aagagcccca gttatcaaac caccgtaaat ggccaaacca ccattccaaa tggcaaaaat
    30121 ctctcctaaa ttctgactat agtaatcaaa tcggaaaata acatagtaga gacgagctcc
    30181 taaaatagcc aagggaaagg ctactaagat aaaatctaaa atatcgtctg gtatgatctt
    30241 ctttctaggt gcttctttca tggtcaaata aaccgcaaga atcaagcctg tcacaataca
    30301 taaggcatac caacgaatgg ctaggggtcc tagttgaata gcaattggat caagcatttt
    30361 gcacctcatt tcgagctatg agacttgtca gtcgttcgtc gaacaaacgg gtagcatcaa
    30421 agcccatttc cttggcacga taattcatgg cagctgcctc aatcacaaca gagatattac
    30481 gacctgtttt aactggaata cgaatacgag gaatggctac gccagaaact tcaagttcct
    30541 ctgcattgtt tccaagacga tcaaaggtct tatgcgtatc gtaattttcc aaatagacag
    30601 caagctgaac ctgtgaagaa tccttgacag cactcgcacc gtagagactc ataacatcga
    30661 taataccaac cccacgaatt tcaatcaagt gtttcaaaat ttcagctggt tcaccccaga
    30721 gagtaatctc atccttggca aagatatcga cacggtcatc ggctaccaaa cggtgaccac
    30781 gtttgacaag ctcaagacct gtctcgctct taccaattcc actatctccc tgaatcaaga
    30841 cgcccatccc ataaatatcc atcaagacac cgtgcacact ggtacgttct gccaaacgag
    30901 aatccagata gctagataac tctccagata aacgactggt agctgcacgg ctggttaaaa
    30961 tagcaatctt acattctcta gcagccttta acatctcctc tggaaccacc aaaccacggg
    31021 caacaatgac tgctggtgtc tcaggtagaa acattttttt caaaacttca taacggctgt
    31081 tggaaggcat gctgatcaga taagaccact ccttcatccc caaaagttga atccgttctg
    31141 gtgtatagta gtcaaaatag cctgtcattt caagaccagg tcgcgtaata tccgctgtat
    31201 tgatttcctt ttcaagcaat tctggttcgc catagacaat gtctagtcta agcttttcaa
    31261 tcacttcttt tactaaaacc gacatcattt cctccttcaa atgagttctt ctctctatta
    31321 tatcaaattg taggaggaag tttcagattt ttgcaggctt tggagtattt atttaaaaga
    31381 aaaaggacta gatttctcta gcctttcatt tcaattagaa ttttttacgt ttacgagcta
    31441 cttctgattt acgtttacgt tttacagaag gtttttcata gaattcacgt ttgcgtgttt
    31501 cttgaagagt accagcttta gtaaccgcac gtttgaaacg gcgaagtgcg tcgtcaagag
    31561 attcattctt acgtactact gttttagaca tttttttcac tcccttcaag ttcaatatct
    31621 atttcattct acacgtaaaa ggaataattg tcaagcaaaa atgcggttta attttgattt
    31681 tttcttccat ttatacaaga gttgaaagcg ttcaatagaa cattgctttt ttatttttcg
    31741 agtaagctaa gcgcttcagc atctgcgatg atggttacat cagggtgatt ttggaggcta
    31801 ctcgcaggta ggttctcagt cactgggcca gatactgttc cggcaatggc ttctgctttc
    31861 gactcaccat aagcaaaaag aataatagac ttggcatcca aaatgttttt aatccccatt
    31921 gaaatagctt gggttgggac gtcttcaatc ttggcaaaga agcgagcatt ggcttcgata
    31981 gtagactggt caagttctac tagatgcgtt tgactgtcaa atggagtacc aggctcatta
    32041 aagccgatat gcccattgcg accgattccc aagatttgga ggtcaactgg atggtcagcc
    32101 aaaatttggt tgtagcgttc aacttcagct tcagcattgt ccttaacacc acgaggcaag
    32161 aaactttctt taaatggttt ttggttgaac aagttttctt gcatgaagta acgataagac
    32221 tgtggattgt ccccatcaag gcccacatac tcatcaaggt tgacactggt tagatttgaa
    32281 aaatcaaggt cactctcaac aatttccttg taaaattcaa gtgggctact tcctgtcgca
    32341 agtcctagag tttgagctcc attagccaat ttttccttca aaatctcaaa agcaactttt
    32401 ccaccttgaa cttggttttc aactttaata actttcatct tttcatcctc catttctgtc
    32461 tatatttatt atatggtata gaccaatttt tgtcaagtga aaacgattta atttctaaaa
    32521 aaagagcgtc caaacttgaa acgtgttata atggatagga ttagaactta gaaagaatga
    32581 acagatatat gaatacagct gattttgatt tccacttgcc agaggaattg attgcccaaa
    32641 cgccccttga aaaacgtgat gcctccaaac tcctcatcgt caaccgtgag acaggagaaa
    32701 tgcaagataa acacttccac tctattattg atatgctgga acctggtgat gcccttgtca
    32761 tgaacgacac ccgagttctc cctgcccgcc tctatggtca aaaagtggag acaggaggtc
    32821 atgtggaact tctcctcctt aagaacacta gtggagacga gtgggaagtt ctggctaaac
    32881 ctgccaaacg cctcaaggtc ggtactcgta tcagctttgg tgatggccgc ctcagcgctg
    32941 tcgttacaga agaattgacc cacgggggac gcattgtccg ctttgaatac caaggaattt
    33001 tcctagaagt cttggaaagt ctgggagaaa tgcctctgcc accttatatc cacgaaaaat
    33061 tagatgaccg tgaacgttat caaaccgtct acgccaagga aagcggctct gctgcagcac
    33121 cgactgctgg tcttcacttc accaaagaac tgctggcaga aatccaagct aagggtgttc
    33181 atctagtcta tctgactctc catgtcggac tcggaacctt tagacctgtt tctgtggata
    33241 atctggacga acacgaaatg cactcagagt tctatcaact ttctgaggaa gctgctgcca
    33301 cccttcgctc tgtcaaaaaa aatggtggtc gtgtcatcgc tgtcggaacc acttctatcc
    33361 gcaccttgga aactattggt tccaagtttg atgggcaaat ccaagcagat tctggttgga
    33421 ccaatatctt tatcaaacct gggtatgagt ggaaggtcgt ggatgccttc tcaaccaact
    33481 tccatctgcc aaaatcaact ctggtcatgt tggtttctgc ctttgcaggc cgtgaattag
    33541 tcttagatgc ctaccaccat gccatccaag aacactaccg cttcttcagt tttggtgacg
    33601 ccatgtttat ttattgagaa agaatttctc taattcttct aataccaata aatcgctaag
    33661 atattatttc aaagaacatc tacaattgaa actctagcta gctgtagaag acgcctagta
    33721 cattgaaatt aaaatgcttc cccctagctt cgaaaatatt gccatagatt gcgttgactc
    33781 tccaaattga ttcatctata ttttatttca gcttcctata ttttcttcgc tgtttgtaaa
    33841 tcaaaatgca agacacatga gtagcaccat atttgttact cttatctgtc ctctcaagag
    33901 actattatga gttatttcag aatcattcac tactttgacc ctgactctcc ttagtctcaa
    33961 aatcaaagac ttatactctt caaaaatctc ttcaaaccgc gtcaacgtca ccttggatta
    34021 tatatgtgac tgacttcgtc agttctatct acaacctcaa agcagtactt tgagcaacct
    34081 gcgactagtt ttctagtttg ctctttgatt ttcattgagt attaaacaaa aagtgaacaa
    34141 atctgaattc taatgtacag aagactaggc ttgttcactt ttttatagtc gctataagat
    34201 gaccttatct atggcttttt atatataatt atatattcag acatactatt atcaattttg
    34261 tcgcagggag gaatctgtta acgcacccat tcaccattat cattgactct atagccatct
    34321 atacttgtat tgaccgctaa ctcacccgat gcatttacat aataccattt accaccaact
    34381 tggaaccatt gattgacttt catagaaccg ttgctgttga ggtagtacca tgaactatta
    34441 acttgtaccc aacctgttgc catggaacca tcagtattat aaaaatacca cataccattt
    34501 tcttgtttcc agtctgttgt tggagcaact gctttagctg gttctactgc tacatctgtt
    34561 ccttggttag atgtaacaga tacaggatac gaaggaatag atgattgctc aggaacaaca
    34621 actttttcag gttctctcgt ccctctcctt atacgtcttt ttaccatctc tttagtaatt
    34681 tgacgagaag tagtttcttc aattgttcca tcacgttcat ctacagtata gattgtagta
    34741 agagtaattt accaatttct cctacttctt ctacttcttg acttttatca agagttgggc
    34801 catcgagata ttctgtttcg attggaattt cttggacaag aacttggggc ttggttcttt
    34861 ttttaacaac tcttgtttga gagtcttttt tttgacttaa agtactctca gttacttgtc
    34921 cactctttcc atctacatta taagttatcg ttgtaactgt tttcccattc tttcctagag
    34981 taatctcttg ctcctgtcct gcagaaaggt cattgtctgc ttcatattta gtagcaaatg
    35041 gaacaagaac ttcttcaacc ttgcttttag ctggaacttt gataactgta tccgtggctt
    35101 cttttctatc aacagtaacc tgttcggtaa cataaccagt ctctggatta acatcgtagg
    35161 tccttgtcgt agttacatag ccatcctctc catcaattgt aacaggattt tcactacggt
    35221 cttttgtttc atctttttca taacgaattc gcgtacttga aattttcttg gttactacct
    35281 taggtttagt cgctactttt acaataatat ccccattgtc agcgtcatca tactctattc
    35341 cctcttcttt atctctagta tcatctctga catattgaat cccatcagca gcatgaacaa
    35401 aacttgtatt cagattcctc ctaaaaataa agttagcccg attaccgcag aaccaaaaat
    35461 ctttccgagt ttacgtattg catagcgctt attagtatta gattttgcca ttacatccta
    35521 cttctagtat agcatctttt cta
//
LOCUS       DAVHEW010000010        34301 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVHEW010000010 DAVHEW010000000
VERSION     DAVHEW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.010"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            530..982
                     /locus_tag="VVL53_000491"
     CDS             530..982
                     /locus_tag="VVL53_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU4182702.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1001..2338
                     /locus_tag="VVL53_000492"
     CDS             1001..2338
                     /locus_tag="VVL53_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU4182703.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2417..3301
                     /locus_tag="VVL53_000493"
     CDS             2417..3301
                     /locus_tag="VVL53_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4182704.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3301..4134
                     /locus_tag="VVL53_000494"
     CDS             3301..4134
                     /locus_tag="VVL53_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4182705.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4152..6245
                     /gene="nanB"
                     /locus_tag="VVL53_000495"
     CDS             4152..6245
                     /gene="nanB"
                     /locus_tag="VVL53_000495"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU4182706.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6257..7360
                     /locus_tag="VVL53_000496"
     CDS             6257..7360
                     /locus_tag="VVL53_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4182707.1"
                     /translation="MVKYGVVGTGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7534..8232
                     /locus_tag="VVL53_000497"
     CDS             7534..8232
                     /locus_tag="VVL53_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU4182708.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8251..9768
                     /locus_tag="VVL53_000498"
     CDS             8251..9768
                     /locus_tag="VVL53_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU4182709.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9893..11221
                     /locus_tag="VVL53_000499"
     CDS             9893..11221
                     /locus_tag="VVL53_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4182710.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLGTIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11473..12339
                     /locus_tag="VVL53_000500"
     CDS             11473..12339
                     /locus_tag="VVL53_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4182711.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12355..13194
                     /locus_tag="VVL53_000501"
     CDS             12355..13194
                     /locus_tag="VVL53_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4182712.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13466..13918
                     /locus_tag="VVL53_000502"
     CDS             13466..13918
                     /locus_tag="VVL53_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU4182713.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14015..14359
                     /locus_tag="VVL53_000503"
     CDS             14015..14359
                     /locus_tag="VVL53_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182714.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVNALIGRYIKLGTY
                     AAKYGISMARSILSRVAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14636..15280
                     /locus_tag="VVL53_000504"
     CDS             14636..15280
                     /locus_tag="VVL53_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU4182715.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15449..16366
                     /locus_tag="VVL53_000505"
     CDS             15449..16366
                     /locus_tag="VVL53_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU4182716.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16384..17268
                     /locus_tag="VVL53_000506"
     CDS             16384..17268
                     /locus_tag="VVL53_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4182717.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17284..18135)
                     /locus_tag="VVL53_000507"
     CDS             complement(17284..18135)
                     /locus_tag="VVL53_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU4182718.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18335..20392
                     /gene="pbp2b"
                     /locus_tag="VVL53_000508"
     CDS             18335..20392
                     /gene="pbp2b"
                     /locus_tag="VVL53_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU4182719.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20403..20999
                     /gene="recR"
                     /locus_tag="VVL53_000509"
     CDS             20403..20999
                     /gene="recR"
                     /locus_tag="VVL53_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU4182720.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21175..22218
                     /locus_tag="VVL53_000510"
     CDS             21175..22218
                     /locus_tag="VVL53_000510"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU4182721.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNNITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPKLIERLVDL
                     AKESFDKREAHLI"
     gene            22302..23675
                     /locus_tag="VVL53_000511"
     CDS             22302..23675
                     /locus_tag="VVL53_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU4182722.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23662..24273
                     /locus_tag="VVL53_000512"
     CDS             23662..24273
                     /locus_tag="VVL53_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4182723.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24364..25068
                     /locus_tag="VVL53_000513"
     CDS             24364..25068
                     /locus_tag="VVL53_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU4182724.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25287..26660
                     /gene="ftsA"
                     /locus_tag="VVL53_000514"
     CDS             25287..26660
                     /gene="ftsA"
                     /locus_tag="VVL53_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU4182725.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26677..27936
                     /gene="ftsZ"
                     /locus_tag="VVL53_000515"
     CDS             26677..27936
                     /gene="ftsZ"
                     /locus_tag="VVL53_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU4182726.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27941..28612
                     /locus_tag="VVL53_000516"
     CDS             27941..28612
                     /locus_tag="VVL53_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU4182727.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28622..29161
                     /locus_tag="VVL53_000517"
     CDS             28622..29161
                     /locus_tag="VVL53_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU4182728.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29161..29424
                     /locus_tag="VVL53_000518"
     CDS             29161..29424
                     /locus_tag="VVL53_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU4182729.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29421..30206
                     /locus_tag="VVL53_000519"
     CDS             29421..30206
                     /locus_tag="VVL53_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU4182730.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30215..31003
                     /gene="divIVA"
                     /locus_tag="VVL53_000520"
     CDS             30215..31003
                     /gene="divIVA"
                     /locus_tag="VVL53_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU4182731.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLVQLAQCMKNQK"
     gene            31351..34143
                     /locus_tag="VVL53_000521"
     CDS             31351..34143
                     /locus_tag="VVL53_000521"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU4182732.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAQSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10529 a         6063 c         7464 g        10245 t
ORIGIN      
        1 agtatttctc tcttaaaagt ttttggtgaa acgagatgta gaaaggagat ttagccaaag
       61 agtctattag tgctagaata atagattaga attattttag aaaaacgaag tgagcagctt
      121 ataaattcaa gtccccaaat agattcatac tagtatcttt tgcaaaaaat aaagggcgac
      181 ttccttcatg aatatcaatt tcatctataa ggaaggtagc taattgaact aacttattta
      241 ttctgtttgt cgctagaaaa atcagacctc cttgtgaaga ttgaggagat acttaatgaa
      301 aatcaaagaa gaaactagca agctagtagc agattgccca aaacaccgct ttgaggttgt
      361 agataagact gacctatata atccaaggtg aagcgactgt ggtttgaaga gattttcaaa
      421 gagtataggc tagagagtag tgtttttatg tccttctagt agaaaatgct agacagaaga
      481 atggggaact tggataggaa aaatagattg agaaaggagg ttagaagaga tgattattac
      541 aaaaattagc cgtttaggaa cttatgtggg agtaaatcca cattttgcaa cattaataga
      601 ttttctagaa aaaacaggac tagaaaattt aacagaaggt tcgattgcta tcgatggtaa
      661 tcgattgttt gggaattgct ttacttatct agcagatggt caagcagggg ctttctttga
      721 aacccaccaa aaatatttgg atattcattt agttttggaa aacgaagaag ccatggctgt
      781 tacatcgccg gaaaatgtaa gcgttaccca agaatatgat gaagagaaag atattgaatt
      841 atacacaggg aaagtggaac agttggttca tttgagagct ggcgaatgcc tcatcacttt
      901 tccagaagat ttacatcaac ccaaggttcg tataaatgat gaacctgtga aaaaagttgt
      961 ctttaaagtt gcgatttctt aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt
     1021 tttatgtcta gctggaattg cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa
     1081 agaagctaca actagtactg aaccaccaac agaattatct ggtgagatta caatgtggca
     1141 ctcctttact caaggacccc gtttagaaag tattcaaaaa tcagcagatg ctttcatgca
     1201 aaagcatcca aaaacgaaaa tcaagattga aacattttct tggaatgact tctatactaa
     1261 atggactaca ggtttagcaa atggaaatgt gccagatatc agtacagctc ttcctaacca
     1321 agtaatggaa atggtcaact cagatgcttt ggttccgcta aatgattcta tcaagcgtat
     1381 tggacaagat aaatttaacg aaactgcctt aaatgaagca aaaatcggag atgattacta
     1441 ctctgttcct ctttattcac atgcacaagt catgtgggtt agaacagatt tgttaaaaga
     1501 acataatatt gaggttccta aaacttggga tcaactctat gaagcttcta aaaaattgaa
     1561 agaagctgga gtttatggct tgtctgttcc gtttggaaca aatgacttaa tggcaacacg
     1621 tttcttgaac ttctacgtac gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc
     1681 agacttgaca agccaacttg ctcaagatgg tattaaatac tgggttaaat tgtataaaga
     1741 aatctcacct caagattctt tgaactttaa tgtccttcaa caagctacct tgttctatca
     1801 aggaaaaaca gcatttgact ttaactctgg cttccatatc ggaggaatta atgccaacag
     1861 tcctcaattg attgattcga ttgatgctta tcctattcca aaaatcaaag agtctgataa
     1921 agaccaagga attgaaacct caaacattcc aatggttgtt tggaaaaatt caaaacatcc
     1981 agaagttgct aaagcattct tagaagcact ttataatgaa gaagactacg ttaaattcct
     2041 tgattcaact ccagtaggta tgttgccaac tattaagggg attagcgatt ctgcagccta
     2101 taaagaaaat gaaactcgta agaaatttaa acatgctgaa gaagtaatta ctgaagctgt
     2161 taaaaaaggt actgctattg gttatgaaaa tgggccaagt gtacaagctg gtatgttgac
     2221 taaccaacac attattgaac aaatgttcca agatatcatt acaaatggaa cagatcctat
     2281 gaaagcagca aaagaagcag aaaaacaatt aaatgattta tttgaggctg ttcagtagat
     2341 gtaaaagact agaaaatagg tgggatagtg agctgaaaag ctctagccca atcttgtaaa
     2401 agaagggaga aggagaatgg ttaaagaacg taatttaact cgctggatat ttgttttgcc
     2461 agctatgatt atcgtaggat tactctttgt ttatccgttt ttctcgagta ttttttatag
     2521 ctttaccaat aagcatttga ttatgcctaa ttataaattt gttggtttgg ctaactataa
     2581 agctgtgcta tcagatccca acttctttaa tgcgttcttt aattcaatta agtggaccgt
     2641 tttctcatta gttggtcaag ttttagtagg gtttgtattg gctttagctc ttcacagagt
     2701 acgccacttc aagaaattat ataggacatt attgattgtt ccttgggcat ttcctaccat
     2761 cgttattgcc ttctcttggc agtggattct aaacggggtt tatggctact tacctaatct
     2821 aatcgtaaaa ttaggtttaa tggaacatac acctgcattt ttgacagata gtacatgggc
     2881 attcctatgt ttggtgttta tcaacatttg gtttggagca ccaatgatta tggttaatgt
     2941 gctttcagct ttgcaaacag taccagaaga acaatttgag gctgctaaga tagatggtgc
     3001 ttcaagttgg caggtgttca agtttatcgt ctttccacat attaaagtgg ttgtaggact
     3061 tctagttgtt ttgagaactg tatggatctt taataacttt gacattatct acctcattac
     3121 tggtggtgga ccagccaatg ctacaacgac gcttccaatt tttgcttaca acctgggctg
     3181 gggaactaaa ttgttgggtc gtgcttcagc agttacagta ctgctcttta tcttcttggt
     3241 ggcgatttgc tttatctact ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata
     3301 atgaagaaga aatccagtat ttatttagat attctctcac atgtactttt agttggtgcg
     3361 accatcgttg cagttttccc attggtatgg attatcatat cttctgtcaa agggaaaggg
     3421 gaattaactc agtatccaac acgattttgg cctgaacagt ttacattaga ttatttcact
     3481 catgttatca acgatttgca cttcattgat aacattcgaa acagtttaat cattgccttg
     3541 gctacaaccc ttattgcgat tattatttct gctatggcag cctatggtat tgttcgattc
     3601 tttcctaaat tgggagcaat catgtcgaga ctactcgtca ttacctacat tttcccacca
     3661 attttgttag caattcccta ttcaattgcc attgctaaag ttgggttaac aaatagttta
     3721 tttggcttga tgatggttta tctatctttt agtgttccat atgcagtttg gctcttagtt
     3781 ggatttttcc aaacagttcc aattggaatt gaagaagcgg ctagaattga tggtgcaaat
     3841 aaatttgtta cgttttataa agttgtgcta ccgattgtag caccaggtat tgtagcaaca
     3901 gctatttata catttatcaa tgcttggaat gaattcctgt atgccttgat tttgattaac
     3961 aatacaggaa agatgacagt agcagtagcc cttcgttcac ttaatggttc agaaatacta
     4021 gactggggag atatgatggc agcgtctgtt attgtagttc ttccatcaat tattttcttc
     4081 tctatcatcc aaaataagat tgcaagtgga ttatcagaag gatctgtgaa gtagacgaaa
     4141 gaaggaaaaa aatgaataaa agaggtcttt attcaaaact aggaatttcc gttgtaggca
     4201 ttagtctttt aatgggagtc cccactttga ttcatgcgaa tgaattaaac tatggtcaac
     4261 tgtccatatc tcctattttt caaggaggtt catatcaact gaacaataag agtatagata
     4321 tcagctcttt gttattagat aaattgtctg gagagagtca gacagtagta atgaaattta
     4381 aagcagataa accaaactct cttcaagctt tgtttggcct atctaatagt aaagcaggct
     4441 ttaaaaataa ttacttttca attttcatga gagattctgg tgagataggt gtagaaataa
     4501 gagacgccca aaagggaata aattatttat tttccagacc agcttcatta tggggaaaac
     4561 ataaaggaca ggcagttgaa aatacactag tatttgtatc tgattctaaa gataaaacat
     4621 acacaatgta tgttaatgga atagaagtgt tctctgaaac agttgataca tttttgccaa
     4681 tttcaaatat aaatggtata gataaggcaa cactaggagc tgttaatcgt gaaggtaagg
     4741 aacattacct cgcaaaagga agtattgatg aaatcagtct atttaacaaa gcaattagtg
     4801 atcaggaagt ttcaactatt cccttgtcaa atccatttca gttaattttc caatcaggag
     4861 attctactca agctaactat tttagaatac cgacactata tacattaagt agtggaagag
     4921 ttctatcaag tattgatgca cgttatggtg ggactcatga ttctaaaagt aagattaata
     4981 ttgccacttc ttatagtgat gataatggga aaacgtggag tgagccaatt tttgctatga
     5041 agtttaatga ctatgaggag cagttagttt actggccacg agataataaa ttaaagaata
     5101 gtcaaattag tggaagtgct tcattcatag attcatccat tgttgaagat aaaaaatctg
     5161 ggaaaacgat attactagct gatgttatgc ctgcgggtat tggaaataat aatgcaaata
     5221 aagccgactc aggttttaaa gaaataaatg gtcattatta tttaaaacta aagaagaatg
     5281 gagataacga tttccgttat acagttagag aaaatggtgt cgtttataat gaaacaacta
     5341 ataaacctac aaattatact ataaatgata agtatgaagt tttggaggga ggaaagtctt
     5401 taacagtcga acaatattcg gttgattttg atagtggctc tttaagagaa aggcataatg
     5461 gaaaacaggt tcctatgaat gttttctaca aagattcgtt atttaaagtg actcctacta
     5521 attatatagc aatgacaact agtcagaata gaggagagag ttgggaacaa tttaagttgt
     5581 tgcctccgtt cttaggagaa aaacataatg gaacttactt atgtcccgga caaggtttag
     5641 cattaaaatc aagtaacaga ttgatttttg caacatatac tagtggagaa ctaacctatc
     5701 tcatttctga tgatagtggt caaacatgga agaaatcctc agcttcaatt ccgtttaaaa
     5761 atgcaacagc agaagcacaa atggttgaac tgagagatgg tgtgattaga acattcttta
     5821 gaaccactac aggtaagata gcttatatga ctagtagaga ttctggagaa acatggtcga
     5881 aagtttcgta tattgatgga atccaacaaa cttcatatgg cacacaagta tctgcaatta
     5941 aatactctca attaattgat ggaaaagaag cagtcatttt gagtacacca aattctagaa
     6001 gtggccgcaa gggaggccaa ttagttgtcg gtttagtcaa taaagaagat gatagtattg
     6061 attggaaata ccactatgat attgatttgc cttcgtatgg ttatgcctat tctgcgatta
     6121 cagaattgcc aaatcatcac ataggtgtac tgtttgaaaa atatgattcg tggtcgagaa
     6181 atgaattgca tttaagcaat gtagttcagt atatagattt ggaaattaat gatttaacaa
     6241 aataaaggag aaaaacatgg ttaaatacgg tgttgttgga acagggtatt ttggagctga
     6301 attggctcgc tacatgcaaa agaatgatgg agcagagatt actcttctct atgatccaga
     6361 taatgcagag gcgattgcag aagaattggg agcaaaagta gcaagttcct tagatgagtt
     6421 ggtttctagc gatgaagtag attgtgttat cgtcgcaact ccaaataatc ttcataagga
     6481 accggttatt aaggctgcac agcatggtaa aaatgttttc tgtgaaaaac caattgcgct
     6541 ttcttatcaa gattgtcgcg agatggtaga tgcgtgtaaa gaaaacaatg taacctttat
     6601 ggcaggacat attatgaatt tctttaatgg tgttcatcat gcaaaagaac tcattaatca
     6661 aggagttatc ggagacgttc tatattgtca tacagctcgt aatggttggg aagaacaaca
     6721 accgtcagta tcatggaaaa aaattcgtga aaaatcaggt ggtcacttgt atcaccacat
     6781 ccatgaattg gattgcgttc aattccttat ggggggcatg cctgaaactg taaccatgac
     6841 aggtggaaat gtggcccatg aaggtgaaca tttcggtgat gaagatgata tgatttttgt
     6901 caatatggaa ttttctaata agcgttttgc cttgttagaa tggggttcag cttatcgttg
     6961 gggtgaacat tatgtcttaa tccaaggaag caaaggtgcc atccgcttag acttattcaa
     7021 ctgtaaagga actcttaagc tagatgggca agaaagctat ttcttgattc acgaatcgca
     7081 agaagaagat gatgatcgga ctcgtatcta tcatagtaca gagatggatg gagcaattgc
     7141 ttatggtaaa ccaggtaaac gtactccatt atggctatca tctgtcattg ataaagaaat
     7201 gcgctatctg catgagatta tggaaggagc tccagtatca gaagaatttg caaaactttt
     7261 gacaggtgaa gctgccctag aagcaattgc tactgcagat gcttgtaccc agtctatgtt
     7321 tgaagatcgc aaagtaaaat tgtcagaaat tgtaaaataa attttggtat tctcctattt
     7381 ataggtcgac ttgctcctct gaaagtactt ttagaggagc tgtttgactt tgctagtttt
     7441 tgaaactgaa atctattata ctacaaacta ttgaaagcgt tttaatttta aggtataata
     7501 atctcataga aataaagaaa aggaggaaag aggatgccac agattagcaa agaagccttg
     7561 attgagcaaa tcaaagatgg aatcatcgtt tcttgtcagg ctcttcctca tgaaccgctt
     7621 tatacagaag cgggaggggt gattcccttg ctggtcaaag cggctgagca aggtggagca
     7681 gtcggtatcc gagcaaacag tgttcgcgat atcaaggaaa ttaaggaagt cactaaactt
     7741 ccaatcattg ggattatcaa acgtgattat ccacctcagg aacccttcat cacggctact
     7801 atgaaagaag ttgatgaatt ggcagaactg gacatcgagg tgattgctct ggattgtacc
     7861 aagcgtgaac gctacgatgg tttggaaatt caagagttca ttcgtcaggt taaggagaaa
     7921 tatcctaatc agcttttgat ggctgatact agtatcttcg aagaagggct agcagctgta
     7981 gaagcaggaa ttgactttgt cggaacaacc ttatcaggct acacatccta cagtccaaaa
     8041 gtagacggtc cagattttga attgattaag aaactctgtg atgctggtgt agatgtcatt
     8101 gcagaaggaa aaattcatac accagaacaa gccaaacaaa tccttgaata tggagtgcga
     8161 ggcatcgttg ttggtggcgc cattactaga ccaaaagaga ttacagaacg cttcgttgct
     8221 agtcttaaat aagatgtgag ggggagtttt atgtttaaag ttttacaaaa agttggaaaa
     8281 gcttttatgt tacctatagc tatacttcct gcagcaggtc tacttttggg gattggtggt
     8341 gcactttcaa acccaaccac gatagcaact tatccaatac tagacaatag tatttttcaa
     8401 tcaatattcc aagtaatgag ctctgcagga gaggttgtat tcagtaattt gtcactactt
     8461 ctctgtgtgg gattatgtat tggcttagcg aaacgagata aaggaaccgc tgcgttagca
     8521 ggagtaactg gttacttagt tatgactgca acgatcaaag ctttggtaaa actttttatg
     8581 gcagaaggat ctgcaattga tactggagtt attggagcat tagttgtcgg aatagttgcc
     8641 gtatatttgc acaaccgata taacaatatt caattacctt ccgctttagg attctttgga
     8701 ggttcacgct tcgttcctat tgttacatcg ttctcttcta tcttgattgg ctttgtcttc
     8761 tttgttattt ggccaccttt ccaacaactt cttgtttcta caggtggata tatttctcag
     8821 gcgggtccaa ttggaacttt tctatatgga tttttaatga gactttctgg agcagtaggc
     8881 ttacatcata taatttaccc tatgttttgg tatactgaac ttggtggtgt tgaaactgtt
     8941 gcaggacaaa cagtggttgg agctcaaaaa atattttttg ctcaattagc cgatttggcc
     9001 cattctggat tatttacaga aggaacaagg ttttttgcag gtcgtttctc aacaatgatg
     9061 ttcggtttac cggctgcctg tttagcgatg taccatagtg ttcctaaaaa tcgtcgtaaa
     9121 aaatacgcgg gtttgttttt tggagttgct ttaacatctt ttattaccgg tattacagaa
     9181 ccaattgaat ttatgtttct attcgtcagt ccggttctat atgttgttca cgcattcctt
     9241 gatggtgtta gcttctttat tgcagacgtc ttaaatattt caataggaaa cacattttca
     9301 ggaggtgtaa tcgatttcac tttatttgga attttgcagg ggaacgctaa gacgaattgg
     9361 gttcttcaga ttccatttgg acttatttgg agtgttttgt attatattat ttttagatgg
     9421 ttcattactc aattcaacgt tctaacgcca gggcgaggag aagaagtaga ttctaaagaa
     9481 atttctgaat ccgcagattc aacttcaaat actgcagatt atttaaaaca ggatagccta
     9541 caaattatca gagccttggg tggatcaaat aatatagaag atgtagatgc ttgtgtgaca
     9601 cgtttacgtg tagctgtaaa agaagttaat caagttgata aagcactttt aaaacaaatt
     9661 ggtgcagttg atgtcttaga agtgaagggt ggcattcaag caatctatgg agcaaaagca
     9721 atcttatata aaaatagtat taatgaaatt ttaggtgtag atgattaagt acttactgac
     9781 ttaataaaaa acagaggaga gtgatggatg agtaggatga aatgaaatcg catacaagaa
     9841 ataaagaact cattatccaa gttggatacg cttattacat aggagaatac aaatgaaatt
     9901 tagaaaatta gcttgtacag tacttgcggg tgctgcggtt cttggtcttg ctgcttgtgg
     9961 caattctggc ggaagtaaag atgctgccaa atcaggtggt gacggtgcca aaacagaaat
    10021 cacttggtgg gcattcccag tatttaccca agaaaaaact ggtgacggtg ttggaactta
    10081 tgaaaaatca atcatcgaag cgtttgaaaa agcaaaccca gatataaaag tgaaattggg
    10141 aaccatcgac ttcaagtcag gtcctgaaaa aatcacaaca gccatcgaag caggaacagc
    10201 tccagacgta ctctttgatg caccaggacg tatcatccaa tacggtaaaa acggtaaatt
    10261 ggctgagttg aatgacctct tcacagatga atttgttaaa gatgtcaaca atgaaaacat
    10321 cgtacaagca agtaaagctg gagacaaggc ttatatgtat ccgattagtt ctgccccatt
    10381 ctacatggca atgaacaaga aaatgttaga agatgctgga gtagcaaacc ttgtaaaaga
    10441 aggttggaca actgatgatt ttgaaaaagt attgaaagca cttaaagaca agggttacac
    10501 accaggttca ttgttcagtt ctggtcaagg gggagaccaa ggaacacgtg cctttatctc
    10561 taacctttat agcggttctg taacagatga aaaagttagc aaatatacaa ctgatgatcc
    10621 taaattcgtc aaaggtcttg aaaaagcaac tagctggatt aaagacaatt tgatcaataa
    10681 tggttcacaa tttgacggtg gggcagatat ccaaaacttt gccaacggtc aaacatctta
    10741 cacaatcctt tgggcaccag ctcaaaatgg tatccaagct aaacttttag aagcaagtaa
    10801 ggtagaagtg gtagaagtac cattcccatc agacgaaggt aagccagctc ttgagtacct
    10861 tgtaaacggg tttgcagtat tcaacaataa agacgacaag aaagtcgctg catctaagaa
    10921 attcatccag tttatcgcag atgacaagga gtggggacct aaagacgtag ttcgtacagg
    10981 tgctttccca gtccgtactt catttggaaa actttatgaa gacaaacgca tggaaacaat
    11041 cagcggctgg actcaatact actcaccata ctacaacact attgatggat ttgctgaaat
    11101 gagaacactt tggttcccaa tgttgcaatc tgtatcaaat ggtgacgaaa aaccagcaga
    11161 tgctttgaaa gccttcactg aaaaagcgaa cgaaacaatc aaaaaagcta tgaaacaata
    11221 gtccttagtt attctataaa aagtagtttt ttaaagaacc taagagtgta tacccccttt
    11281 tccctctaca cagatagtgt aagaaaaggg ggcttttgtt taaaatgtaa gaaactgtca
    11341 cgaaattaaa atgaagttct tacataagcg aatcataaaa aatttcattt tgattttaaa
    11401 acagttcaag aaagtcaaaa aattattcta tttgaaagag aggtgccgac tgtgaaagtc
    11461 aataaaatcc gtatgcggga aacagtgatt tcctacgctt tcctagcacc agtattattc
    11521 ttctttgtca tctttgtgtt ggctccgatg gtgatgggct tcattacaag tttctttaac
    11581 tactcaatga ctaaatttga gtttgtaggc ttggataact atatccgtat gtttaaagat
    11641 cctgtcttta caaaatctct gattaacaca gttattttgg ttattggatc tgtaccagtt
    11701 gttgttctat tctcactctt tgtagcatct cagacctatc atcaaaatgt cattgccaga
    11761 tccttctacc gtttcgtctt cttccttcct gttgtaacgg gtagtgttgc cgtgacagtt
    11821 gtttggaaat ggatttatga cccactatca gggattctaa actttgtcct taagtccagc
    11881 cacatcatca gccaaaacat ttcttggttg ggagataaaa actgggcatt gatggcgatt
    11941 atgattattc tcttgaccac ttcagttggt cagcccatca tcctttatat cgctgccatg
    12001 gggaatattg acaattcact ggttgaagcg gcgcgtgttg atggtgcaac tgagtttcaa
    12061 gttttttgga agattaaatg gccaagcctt cttccaacaa ctctttatat tgcaatcatc
    12121 acaacaatta actcattcca gtgtttcgcc ttgattcagc ttttgacatc tggtggtcca
    12181 aactactcaa caagtacctt gatgtactac ctttacgaaa aagccttcca attgacagaa
    12241 tacggctatg ccaacacaat tggtgtcttc ttggcagtca tgattgctat cgtaagcttt
    12301 gttcaattta aagtacttgg aaacgacgta gaatactaaa gaaaggagac agctatgcaa
    12361 tctacagaaa aaaaaccatt aacagccttt actgttattt caacaatcat tttgctcttg
    12421 ttgactgtgc tgttcatctt tccattctac tggattttga caggggcatt caaatcacaa
    12481 cctgatacaa ttgttattcc tcctcagtgg ttccctaaaa tgccaaccat ggaaaacttc
    12541 caacaactca tggtgcagaa ccctgccttg caatggatgt ggaactcagt atttatctca
    12601 ttggtaacca tgttcttagt ttgtgcaacc tcatctctag caggttatgt attggctaaa
    12661 aaacgtttct atggtcaacg cattctattt gctatcttta tcgctgctat ggcgcttcca
    12721 aaacaagttg tccttgtacc attggtacgt atcgtcaact tcatgggaat ccatgatact
    12781 ctctgggcag ttatcttgcc tttgattgga tggccattcg gtgtcttcct catgaaacag
    12841 ttcagtgaaa atatccctac agagttgctt gaatcagcta aaatcgacgg ttgtggtgag
    12901 attcgtacct tctggagtgt agccttcccg attgtgaaac cagggtttgc agcccttgca
    12961 atctttacct tcatcaatac ttggaatgac tacttcatgc aattggtaat gttgacttca
    13021 cgtaacaatt tgaccatctc acttggggtt gcgaccatgc aggctgaaat ggcaaccaac
    13081 tatggtttga ttatggcagg agctgccctt gctgctgttc caatcgtcac agtcttccta
    13141 gtcttccaaa aatccttcac acagggtatt actatgggag cggtcaaagg ataatactct
    13201 gcgaaaatct cttcaaacta cgtcagcttc accttgccat acttaagtat tgcctgcggt
    13261 tagcttccta gtttgttctt caattttcat tgagtatagg aaaatcaatc tatcaagata
    13321 cagaagtata ttttatagat ttagagaata tagaggttat aagtgtctac aaaatggagg
    13381 gtatgcagtt actttatgaa gttttgtcag acacttataa acttaagaat ggttttagtt
    13441 aactatcaga aacgaaggaa agagtatgat ttttgacgat ttgaaaaaca tcacctttta
    13501 caaagggatt catcctaatt tagacaaggc tatcgactat ctctaccaac atcgtaagga
    13561 ttctttcgaa ttaggaaagt atgatattga tggagataaa gtctttctag ttgttcagga
    13621 aaatgtcctc aatcaagctg aaaatgatca atttgagtat cataagaact atgcagattt
    13681 gcatttgctg gtagaaggac atgaatattc gagctacggt tcacgtatca aagacgaggc
    13741 agtagcattc gacgaagcga gtgacattgg ctttgttcat tgtcatgaac actacccact
    13801 cttgttgggt tatcacaatt ttgcgatttt cttcccaggt gagccacatc agccaaatgg
    13861 ttatgcaggc atggaagaaa aggttcgaaa atatctcttt aaaattttga ttgattaaaa
    13921 ataggatgaa ttgttttttt gtaaagcttt gataatactc taccatgaaa ttgatctttg
    13981 tgaggtagag aaatgagaat aaaatattta aaaattggta tcttctaagt atgctgcaag
    14041 agctagtttc ttagatggac aggggattac agttgatgag atggcttgga taattagggg
    14101 cattgtgaat gcattgattg gtagatacat aaaattaggt acttatgcgg ctaagtatgg
    14161 tattagtatg gcacgctcga tcttaagtag ggtagctgca actgcagcag caagagtagg
    14221 attactgacc aagatttctg gatggatttt acgagtagct gtgaatgtag ctgatgtata
    14281 tggtaatttt gccaacaata ttgctgcagc ttgggatgca tatgataaaa ttcctaacaa
    14341 tggtcgtata aacttttaaa atgcgagaat gaaagcactt tgtatttttt tattgaatat
    14401 gttagcttgg acagtgcttg caatgataat tcgtggaggg ctagatggat ttgataggca
    14461 tacttggagt actattttaa ttgcttcgct gttcggggta tatgattata agcccataga
    14521 taaaaataga aaaaagtcca aaagaaaaaa tagatttgtt catggtaggg acttatgaaa
    14581 gctttactga caaaaaagaa aacagtttac aaagaaaaat gatggaggag caaacatggc
    14641 acaaaaagga gtaagcctta tcaaggcagc atttgataca gataactttc tcatgcgttt
    14701 tagtgagaag gtcttggaca tcgtgacagc caatcttctt tttgtcgtct cttgtttacc
    14761 catcgtgacg attggagtgg ctaaaatcag cctctacgag accatgttcg aagttaagaa
    14821 gagcagacgg gtgcctgttt ttaaaatcta tctaagatct ttcaagcaaa atctgaaact
    14881 aggtcttcag ctgggtttaa tggagttagg aattgtgttt cttacccttt cagatctcta
    14941 tcttttctgg ggtcaaacag ctctgccctt ccaattgctg aaagccattt gtttaggtat
    15001 tctgattttt cttactatcg tgatgctggc tagttaccct atcgcggcac gttatgacct
    15061 atcttggaaa gaaattcttc aaaaaggatt gatgttggct agttttaact ttccttggtt
    15121 cttcctcatg ttagccattc ttgtcctcat tgtgatggtt ctttatctgt ccgccttcag
    15181 tctactctta ggtggctcag tcttcctact ttttgggttt ggactattgg tctttatcca
    15241 gactggattg atggagaaaa ttttcgcaaa ataccaatag gagctttatt tctgaaacta
    15301 ctttcaaagg ctccaaacgc tattctataa gcgagaaact aaaatctaag tgtctataag
    15361 atattgaaag ccttttcggt aaagtgtata ctaaagaagt aaagaaaaat ctgctttagc
    15421 agaaaataaa aataatagga gaaaatctat gtcagatttg aaaaaatacg aaggtgtcat
    15481 tccagccttc tacgcatgtt atgatgatca aggagaagta agcccagaac gtacgcgtgc
    15541 cttggttcaa tacttcattg ataaaggtgt tcaaggtctt tatgtcaatg gttcttctgg
    15601 tgaatgtatc taccaaagcg ttgaagatcg caagttgatt ttggaagaag tcatggcggt
    15661 agccaaaggt aaattgacca ttattgccca tgttgcttgc aataatacta aagatagtat
    15721 ggaacttgct cgccatgctg aaagcttggg agtagatgct attgcaacaa ttccaccaat
    15781 ttatttccgc ttgccagaat actcagttgc caaatactgg aacgatatca gttctgcagc
    15841 tccaaacaca gactacgtga tttacaacat tcctcaattg gcaggggttg ctttgactcc
    15901 aagcctttac acagaaatgt tgaaaaatcc tcgtgttatc ggtgtgaaga actcttctat
    15961 gccagttcaa gatatccaaa cctttgtcag ccttggtgga gaagaccata tcgtctttaa
    16021 tggtcctgat gagcagttcc taggaggacg cctcatgggg gctagggctg gtatcggtgg
    16081 tacttatggt gctatgccag aactcttctt gaaactcaat cagttgattg cggataagga
    16141 cctagaaaca gcgcgtgaat tgcagtatgc tatcaacgca atcattggta aactcacttc
    16201 tgctcatgga aatatgtacg gtgtcatcaa agaagtcttg aaaatcaatg aaggcttgaa
    16261 tattggatct gttcgttcac cattgacacc agtgactgaa gaagatcgtc cagttgtaga
    16321 agcggctgct gccttgattc gtgaaaccaa ggagcgcttc ctctaatcta aaaggaggta
    16381 tttatgacat attacgttgc aattgatatc ggtggaacca acatcaagta tggtttggtt
    16441 gatcaagagg ggcaacttct tgaatcgcat gaaatgccaa ctgaggcgca taagggtgga
    16501 cctcatatct tacaaaagac caaagatatc gtagctagtt atttagaaaa aggcccagta
    16561 gcaggtgttg ccatatcttc tgctgggatg gtggatccgg ataagggtga gattttctat
    16621 gctgggccgc aaatccctaa ctacgcaggc acccagttca aaaaggaaat cgaagaaagc
    16681 tttactattc cttgtgagat tgaaaatgat gtcaactgtg caggtcttgc tgaggcagta
    16741 tctggttcag gcaagggagc aagtgtgaca ctttgcttga ccattggaac cggtatcggt
    16801 ggttgcttga ttatggatag gaaagtcttc catggtttta gcaattcagc ctgtgaagtc
    16861 gggtatatgc atatgcagga tggagctttt caagacttgg cttctacaac agctttagtg
    16921 aaatatgtag ctgaagccca tggagaagat gttgatcagt ggaatggccg tagaattttc
    16981 aaagaagcca ctgaaggaaa caaaatctgc atggaaggta ttgaccgtat ggttgactat
    17041 ctaggaaaag gtctggcaaa tatttgctac gttgccaatc cagaagtggt tattcttggt
    17101 ggtggtatca tggggcaaga ggctatcctc aaacctaaga tccgtacagc cttgaaagag
    17161 gctttggtac caagtttagc agaaaaaaca cgattagaat ttgcccatca ccaaaataca
    17221 gcagggatgt tgggtgcata ttatcatttt aagacaaaac aatcctagtt tggctcagcc
    17281 aaactaggat tttcttacac gtttttgtct acgatagccg ttgagttttt tattttccca
    17341 gtagctatta aagatttttt ccttgctttc gcgattgatt tccaaaaagt aggcataaat
    17401 caaatcgata aagaagagca taggaagttg agcggatatt cgttggatat aggagggttg
    17461 gctgtgggtg gctacaagaa cagtctctgt ataggtctgg ctatctttat tgggaacact
    17521 tgtaaagagt acagtctttg cccccatctc cttagcatct aatagactat ctaaaataga
    17581 aggagttgag cctgaaagtg agaagcccag tactagacaa ttttcatcca tgatgctggt
    17641 tgtccaggca aagccgtctt ggtctgtcaa agcttcgcag accacaccta gtcgcataaa
    17701 acgtaatttc atttcacggg cgacgaggcc agaactccct gttccaaaga agtagatacg
    17761 ctcagcatct tcgattagct gggcaattcg ttctagttgg atttcgtcaa tcaagtcttg
    17821 tgtttgttcc ctcatattgc tataacttct gaggactcgt ttggtcagtg gactgtgctt
    17881 ggagacttgg ttggcttgat tttctgcctg atgttggtat tggaaaataa attctcggta
    17941 gccagtaaag ccacactttt tagcaaagcg ggtcaaagca gcttgagaaa tatgtaattt
    18001 ttgggtgact tgttgagaag ataaatcatc tgtaatcgtt tcagcttgca aaaaatagcg
    18061 agcgatttct tgttctaggt ctgtcatttc ttcaaaatgt gaatcaatga tagttgcgat
    18121 atctggtttg tccataggga aagctccttt acatgagtca tactggaaga ctagatcaga
    18181 gaatagtcac acttcattat aacacataat ataaggatag ataaataaaa acgcatctct
    18241 gttttaaaaa cgaaaaaatc gaaaaagctt ctctcttttc cataattttc tactcaaatt
    18301 gtggtacaat taagagtaag attttaagtt agaaatgaga ctgatttgta tgagaaaatt
    18361 taacagccat tcgattccga ttcggcttaa tttattgttt tcaatcgtca ttttactctt
    18421 tatgaccatt attggtcgtt tgttgtatat gcaggttttg aacaaggatt tttacgaaaa
    18481 aaagctagct tcagctagtc agaccaagat tacaagcagt tcagcccgtg gggaaattta
    18541 tgatgctagt ggaaaacctt tggtagaaaa tacgttaaag caggttgttt cctttacgcg
    18601 tagcaataaa atgacggcta cagacttaaa agaaacagct aaaaagttac tgacttatgt
    18661 gagcatcagt tctccaaatt tgacagaacg ccagctggcg gattactatt tggctgatcc
    18721 tgaaatctat aaaaaaatag tggaagctct cccaagtgag aaacgcttgg attcagatgg
    18781 caatcgtcta tccgaatcag aactgtataa caatgcggtc gatagtgtac aaacgagtca
    18841 actaaactat acagaggatg aaaagaaaga aatctatctt tttagtcagt taaatgctgt
    18901 tggaaacttt gcgacaggaa ccattgcgac agatcctcta aatgattctc aggtggctgt
    18961 tattgcctct atttcaaagg agatgcctgg cattagtatt tctacttctt gggatagaaa
    19021 ggttttggaa acttcccttt cttctatagt tgggagtgta tccagtgaaa aagctggtct
    19081 cccagcggaa gaagcagaag cctatcttaa aaaaggctat tctctaaatg accgtgtagg
    19141 aacctcctat ttggaaaagc aatatgaaga gaccttacaa ggaaaacgct cggtaaaaga
    19201 aatccatctg gataaatatg gcaatatgga aagcgtggat acaattgagg aaggtagtaa
    19261 gggaaacaat atcaaactga ccattgattt ggctttccaa gatagcgtgg atgctttact
    19321 gaaaagttat ttcaattctg agctagaaaa tggtggagcc aagtattctg aaggtgtcta
    19381 tgcagtcgcc cttaacccaa aaacaggtgc ggttttgtct atgtcaggga ttaaacatga
    19441 cttgaaaacg ggagagttga cgcctgattc cttgggaacg gtaaccaatg tctttgttcc
    19501 aggttcggtt gtcaaggcgg cgaccatcag ctcaggttgg gaaaatggag tcttgtcagg
    19561 aaaccagacc ttgacagacc agtccattgt cttccaaggt tcagctccca tcaattcttg
    19621 gtatactcag gcttacggtt cattccctat cacagcggtc caagctctgg agtattcatc
    19681 aaatacctat atggtccaaa cagccttagg tcttatgggg caaacctatc aacccaatat
    19741 gtttgtcggc accagcaatc tagagtctgc tatggagaaa ctgcgttcaa cctttggcga
    19801 atatggcttg ggtactgcga caggaattga cctaccagat gaatctactg gatttgttcc
    19861 caaagagtat agctttgcta attacattac taatgccttt gggcagtttg ataactatac
    19921 gccgatgcag ttggctcagt atgtagcaac tattgcaaat aatggtgttc gtgtggctcc
    19981 tcgtattgtt gaaggcattt atggtaataa tgataaggga ggactgggtg acttgattca
    20041 gcaactgcaa ccgacagaga tgaataaggt caatatatcc gactccgata tgagcatctt
    20101 gcaccaaggt ttttatcagg ttgcccatgg tactagtgga ttgacaactg gacgtgcctt
    20161 ttcaaatggt gccttggtat ccattagcgg aaaaacaggt acagccgaaa gctatgtggc
    20221 agatggtcag caagcaacca ataccaatgc ggtggcctat gccccatctg ataatcccca
    20281 aatcgctgtc gcagtggtct ttcctcataa taccaatcta acaaatggtg taggaccttc
    20341 cattgcgcgt gacattatca atctgtatca aaaataccat ccaatgaatt agaaaggaaa
    20401 ttatgcttta tccaacacct attgccaagt tgattgacag ttattctaag ttaccaggta
    20461 tcgggattaa gacggctacg cgtctggcct tttatacgat tgggatgtct gctgatgatg
    20521 tcaatgaatt tgcaaaaaat ctcctttctg ctaagagaga attgacatat tgttctattt
    20581 gtggacgttt gacagacgac gatccttgtt ctatctgtac tgatccgact cgtgaccaga
    20641 caacaatttt agttcttgag gatagtagag atgtggcagc catggaaaat atccaagaat
    20701 accatggact ctatcatgtc cttcatggcc tcatttctcc tatgaatggt atcagtccgg
    20761 acgatatcaa tctcaagagc cttatgactc gtcttatgga tagtgaggtt tcagaagtga
    20821 ttgtggcgac taatgctaca gcggatggtg aagcgacttc catgtatctt tcacgtttgc
    20881 tcaagccggc tggtatcaag gttacgcgtc tagcacgagg tctcgctgtg ggagcggaca
    20941 ttgagtatgc ggacgaagtg acactcttac gagccattga aaatcggaca gagttgtaag
    21001 tgtaggcaaa tttacgaact ccattcattt ataaaaaatc aaagaggctg aaaatcgttc
    21061 ctatcggcct ctttttgtat agtgtgatga gtaggctcag gttcaagttt taaaaaacca
    21121 agcaaatatg atatactaaa gagcgagtat tctagtagaa ttaggacaaa taatatgaaa
    21181 caaacgatta ttcttttata tggtggacgg agtgcggaac gcgaagtctc tgtcctttca
    21241 gctgagagtg tcatgcgtgc ggtcgattac gaccgtttca cagtcaagac tttctttatc
    21301 agtcagtcag gtgactttat caaaacacag gaatttagtc atgctccggg gcaagaagac
    21361 cgtctcatga ccaatgaaac cattgattgg gataagaaag ttgcaccaag tgctatctac
    21421 gaagaaggtg cagtggtctt tccagtcctt cacgggccaa tgggagaaga tggctctgtt
    21481 caaggattct tggaagtttt gaaaatgcct tacgttggtt gcaacatttt gtcatcaagt
    21541 cttgccatgg ataaaatcac gactaagcgt gttctggaat ctgctggtat tgcccaagtt
    21601 ccttatgtgg ctatcgttga aggcgatgat gtgactgcta aaatcgctga agtggaagaa
    21661 aaattggctt atccagtctt cactaagccg tcaaacatgg ggtctagtgt cggtatttct
    21721 aagtctgaaa accaagaaga actccgtcaa gccttaaaac ttgccttccg atatgacagc
    21781 cgtgtcttgg ttgagcaagg agtgaatgcc cgtgaaattg aggttggcct cttgggtaac
    21841 tacgatgtca agagcacgct accaggagaa gttgtcaagg acgttgcctt ttatgactac
    21901 gatgccaagt atattgataa caatattact atggatattc ctgccaaaat cagtgatgat
    21961 gtggtggctg tcatgcgtca aaatgcagaa acagccttcc gtgccattgg tggccttggt
    22021 ctatctcgtt gcgatttctt ctatacagat aagggagaga tttttctcaa cgagctcaat
    22081 actatgccag gtttcaccca gtggtctatg tacccactac tttgggacaa tatggggatc
    22141 agctacccaa aactaatcga gcgtttggtt gaccttgcca aggaaagttt tgacaagcgc
    22201 gaagcgcatt tgatataaaa tgaaagagag ggtagaagcc agaaccatca ctgcacggtg
    22261 actagagttc tcggacttca gcccttttta aaggagtaga aatgaaatta acaatccatg
    22321 aaattgccca agttgttgga gccaaaaatg atatcagtat ctttgaggac acccagttag
    22381 aaaaagctga gtttgatagt cgtttgattg gaactggaga tttatttgtg ccacttaaag
    22441 gtgcgcgtga tggccatgac tttattgaaa cagcctttga aaatggtgca gcagtaacct
    22501 tgtctgagaa agaggtctca aatcatcctt acattctagt agatgatgtt ttgacagcct
    22561 ttcaatcctt agcatcctac tatcttgaaa aaacgactgt tgatgtcttt gctgttacag
    22621 gttcaaatgg caagacaacg actaaggata tgttggcgca tttactgtca acaagataca
    22681 agacctacaa aacacaaggc aattacaata atgagattgg ccttccttac acagttcttc
    22741 atatgcctga aggaacagaa aagttggttt tggagatggg acaggatcac ttgggcgata
    22801 ttcatctctt gtctgaattg gctcgtccaa aaacagccat cgtgaccttg gttggagaag
    22861 cccatttggc ctttttcaaa gaccgttcag agattgctaa gggaaaaatg caaattgcag
    22921 acggaatggc ttcaggttcc ttgcttttag cgccggctga ccctatcgta gaggactatt
    22981 tgccaactga taaaaaggtg gttcgttttg ggcaaggggc agagctggaa attactgact
    23041 tggttgagcg caaagatagt ctgaccttca aggccaattt cttagagcaa gcccttgatt
    23101 tgccagtaac tggcaagtac aatgcgacaa atgctatgat tgcatcctat gttgccttgc
    23161 aagaaggagt ttcagaggag caaattcgtt tggccttcca agatcttgaa ttgacgcgta
    23221 accgtaccga gtggaagaaa gcagccaatg gagcagatat cctgtcagat gtttacaatg
    23281 ccaatccaac tgctatgaaa ctgattttag agactttctc tgccattcca gccaatgaag
    23341 gtggcaagaa aattgcagtg ttggcggata tgaaggagct tggtgaccag tctgttcaac
    23401 ttcataatca gatgattttg agcctttctc cagatgtgct tgataccgtg attttctatg
    23461 gagaaaatat tgctgaatta gcccaattgg ccagtcaaat gttcccaatc ggccacgttt
    23521 actacttcaa gaaaacagaa gaccaggatc aatttgaaga cctagtcaag caggtcaagg
    23581 aaagccttgg agcccatgac caaatcctgc tcaaaggctc taactctatg aatctagcca
    23641 agttggtaga aagtttagaa aatgaagaca agtgattttg tcaagtattt gcaaagaatg
    23701 attgccatta cagatactgg cttaaccttt acaaaagatc cgtttgaccg tgagcgctac
    23761 gaagacttgc gaagtctgtt atctgaaatg ttgaatcaag catcagacct tgattccgaa
    23821 gaagtggcag aagtcttgaa gccaacttct gcttatgcga ctccgttaat ggacgtccgt
    23881 gcttggattg ttgaggatga gaagatttgt ctggttaggg gacaaggaga ggatagttgg
    23941 gctttgccag gtggctttgg tgaagtcggc tattctccaa cagaaaatat tctcaaggaa
    24001 attgaagaag aaaccggttt taaagccaaa gttgaaagac tgctagctgt ttttgatacc
    24061 aatcgtttcc aactacagag caaacagtat acaaagtttg tttttggatg taagcttctt
    24121 gatggacaat tccaagaaaa tcaagaaatt gctgaccttc aattttttgc cattgaccaa
    24181 ctgccgaact tatctgaaaa acgcattacc aaggagcaaa tagagcttct ttggcaggtt
    24241 tatcaaggtc atagggggca atatcttgac taagaagatg attatcgtat ttctaaatcc
    24301 atttttaaca actagcatgg tataataata tgcaggaaaa ttttgaatta tgaggaagac
    24361 tagatgaatt tatgggatat tttctttacg actcaggcaa ccgagccgcc caaatttgac
    24421 cttttttggt atgttagcct atttacgctc ttagccttaa ccttttatac agcccatcgc
    24481 tatcgtgaaa agaaggttta ccaacgattt ttccaaatct tgcagactgt tcagttaatc
    24541 cttctttatg gttggtactg ggtcaatcat atgccactgt cagaaagcct acccttttac
    24601 cattgccgta tggctatgtt tgtggtactc ttgcttcctg gtcaatccaa atataaacaa
    24661 tactttgcat tattgggaac atttgggaca ttagcagcct ttgtttatcc agtgccagat
    24721 gcttaccctt ttccacatat caccattcta tcctttatct ttggtcattt agcactcttg
    24781 gggaactctc tagtttatct attgagacag tataatgcgc gattgctgga tgtgaaggga
    24841 atttttctca tgacctttgc cctaaatgcc ttgatttttg tggtcaattt ggtgacaggt
    24901 ggcgattacg gatttttgac aaaaccgcca ttggttgggg atcacggtct agtagctaat
    24961 tatttacttg tttcaattgt gctggtagct actatcagtt tgactaagaa aatcttagaa
    25021 ttctttttag ctcaagaagc agaaaaaatg attgcaaagg aagcttaaca cagagctttc
    25081 ttttttgctc ttagagagtt tttacaagca gcttataaaa taagaatttc tgaatagaca
    25141 aactcaaaaa atggctggga aatttaggaa aaaagcaagc acgattaaat tttttgtgtt
    25201 ataatatttt gtgaatagct atgcctatgt ttagctatgg aataatacga agtgcgaaac
    25261 ttggaagata gagaggaagc gatgtaatgg ctagagaagg cttttttaca ggtctagata
    25321 ttggaacaag ctctgtcaag gtgcttgtgg ccgagcagag aaatggtgaa ttaaatgtaa
    25381 ttggcgtgag taatgccaaa agtaaaggtg taaaggatgg aattattgtt gatattgatg
    25441 cagcagcaac tgctatcaag tcagccattt cccaagcgga agaaaaggca ggcatttcga
    25501 ttaaatcagt gaatgtcggc ttgcctggta atcttttgca ggtagaacca actcagggga
    25561 tgattccagt aacatctgat actaaggaaa ttacggatca agatgttgaa aatgttgtca
    25621 aatcagcttt gacaaagagt atgacacctg accgtgaagt cattaccttt attcctgaag
    25681 aatttattgt ggatggtttc caagggattc gtgacccacg tggcatgatg ggggttcgcc
    25741 ttgaaatgcg tggtttgctt tatacaggac ctcgtactat cttgcacaat ttgcgtaaga
    25801 cggttgagcg tgcaggtgtt caggttgaaa atgttatcat ttcaccacta gcaatggttc
    25861 agtctgtttt gaacgaaggg gaacgtgaat ttggtgctac agtgattgat atgggggcag
    25921 gtcaaacgac tgtcgctaca atccgtaatc aagaactcca gttcacacat attctccaag
    25981 aaggtggaga ttatgtaact aaagatatct ccaaggtttt gaaaacctct cgcaaattag
    26041 cggaaggctt gaaactgaat tacggggaag cctatccgcc tcttgcaagc aaagaaacct
    26101 tccaagtaga ggttattgga gaagtagaag cagtcgaagt gacggaagcc tacttgtcag
    26161 aaattatttc tgcacgaatc aagcacatcc ttgaacaaat caagcaagaa ttagatagaa
    26221 ggcgtctatt ggacctccct ggtggtattg tcttaatcgg tgggaatgcc attttaccag
    26281 gtatggttga gcttgctcag gaagtctttg gcgtccgtgt caagctttat gttccaaatc
    26341 aagttggtat ccgtaatcca gcctttgcgc atgtgattag tttatcagaa tttgcgggtc
    26401 aattaacaga agttaatctt ttggctcagg gagcgataaa aggtgagaat gacttaagtc
    26461 atcagccaat tagttttggt gggatgctgc aaaaaacagc tcagtttgta caatcaacgc
    26521 ctgttcaacc agctcctgct ccagaagtag agccggtggc gcctacagaa ccaatggcgg
    26581 atttccaaca agcttcacaa aataaaccga aattagcaga tcgtttccgt ggattgatcg
    26641 gaagcatgtt tgacgaataa agaggaaaaa taaattatga cattttcatt tgatacagct
    26701 gctgctcaag gggcagtgat taaagtaatt ggtgtcggtg gaggtggtgg caatgccatc
    26761 aaccgtatgg tcgacgaagg tgttacaggc gtagaattta tcgcagcaaa cacagatgta
    26821 caagcattga gtagtacaaa agctgagact gttattcagt tgggacctaa attgactcgt
    26881 ggtttgggtg caggaggtca acctgaggtt ggtcgtaaag ccgctgaaga aagcgaagaa
    26941 acactgacgg aagctattag tggtgccgat atggtcttca tcactgctgg tatgggagga
    27001 ggctctggaa ctggagctgc tcctgttatt gctcgtatcg ccaaagattt aggtgcgctt
    27061 acagttggtg ttgtaacacg tccctttggt tttgaaggaa gtaagcgtgg acaatttgct
    27121 gtagaaggaa tcaatcaact tcgtgagcat gtagacactc tattgattat ctcaaacaac
    27181 aatttgcttg aaattgttga taagaaaaca ccgcttttgg aggctcttag cgaagcggat
    27241 aacgttcttc gtcaaggtgt tcaagggatt accgatttga ttaccaatcc aggattgatt
    27301 aaccttgact ttgccgatgt gaaaacggta atggcaaaca aagggaatgc tcttatgggt
    27361 attggtatcg gtagtggaga agaacgtgtg gtagaagcgg cacgtaaggc aatctattca
    27421 ccacttcttg aaacaactat tgacggtgct gaggatgtta tcgtcaacgt tactggtggt
    27481 cttgacttaa ccttgattga ggcagaagag gcttcacaaa ttgtgaacca ggcagcaggt
    27541 caaggagtga acatctggct cggtacttca attgatgaaa gtatgcgtga tgaaattcgt
    27601 gtaacagttg ttgcaacggg tgttcgtcaa gaccgcgtag aaaaggttgt ggctccacaa
    27661 gctagatctg ctactaacta ccgtgagaca gtgaaaccag ctcattcaca tggctttgat
    27721 cgtcattttg atatggcaga aacagttgaa ttgccaaaac aaaatccacg tcgtttggaa
    27781 ccaactcagg catctgcttt tggtgattgg gatcttcgcc gtgaatcgat tgttcgtaca
    27841 acagattcag tcgtttctcc agtcgagcgc tttgaagccc caatttcaca agatgaagat
    27901 gaattggata cacctccatt tttcaaaaat cgttaagtaa atgaatgtaa aagaaaatac
    27961 agaacttgtt tttcgagaag ttgcagaggc tagtctgagt gctcatcgag agagtggttc
    28021 ggtctctgtc attgcagtta ccaagtatgt agatgtaccg acagcggaag ccttgcttcc
    28081 gctaggtgtc catcatatcg gtgaaaatcg tgtagataag tttctggaaa aatatgaagc
    28141 tttaaaagat cgagatgtga cttggcattt gattggtacc ttgcaaagac gtaaggtgaa
    28201 agatgtcatt caatacgttg attatttcca tgcattggac tcagtaaagc tagcagggga
    28261 aattcaaaaa agaagtgacc gagtcatcaa gtgtttcctt caagtaaata tttctaaaga
    28321 agaaagcaaa cacggttttt cgagagagga actgctggaa atcttgccag agttagccag
    28381 actagataag attgaatatg ttggtttaat gacgatggca ccttttgagg ctagcagtga
    28441 gcagttgaaa gagattttca aggcggccca agatttacaa agagaaattc aagagaaaca
    28501 aattccaaat atgcctatga ccgagttaag tatgggaatg agtcgtgatt ataaagaagc
    28561 gattcaattc ggttccactt ttgttcgtat aggtacatca ttttttaagt aggagagaac
    28621 catgtcttta aaagatagat tcgatagatt tatagattat tttacggagg atgaggattc
    28681 aagtctccct tatgaaaaaa gagatgagcc tgtgtttact tcagtaaatt cttcacagga
    28741 accggctctc ccaatgaatc aaccttcaca gtcggctggc acaaaagaga acaatatcac
    28801 cagacttcat gcaagacaac aggaattggc aaatcagagt cagcgtgcaa cggataaggt
    28861 cattatagat gttcgttatc ctagaaaata tgaggatgca acagaaattg ttgatttatt
    28921 ggcaggaaac gaaagtatct tgattgattt tcagtatatg acagaggtgc aggctcgtcg
    28981 ttgtttggac tatttggatg gagcttgtca tgttttagct ggaaatttga aaaaggtagc
    29041 ttctaccatg tatttgttga caccagtgaa cgttattgta aatgttgaag atatccgttt
    29101 accagatgaa gatcaacagg gtgagttcgg ttttgatatg aagcgaaata gagtacgata
    29161 atgatttttt taattcgtat gatttataat gcagtggata tttactccct gattttggta
    29221 gccttcgctg tcatgtcttg gtttccaggt gcctacgaat ccagtttagg tcgttggatt
    29281 gtagcgttgg tgaaaccagt gcttgctccc ttgcaacgcc tgcctttaca gatagcgggt
    29341 cttgatttat ctgtttgggt tgcgattgtt ttggttcgat ttttaggaga aaacctagtg
    29401 cgttttctgg cgatgatagg atgaataaag ggatttatca gcatttctcc atagaagatc
    29461 gtccatttct tgacaaggga atggaatgga taaagaaggt agaagatagc tatgctcctt
    29521 ttttaactcc ttttatcaat cctcatcagg agaagctatt aaagattttg gccaaaacct
    29581 atggtcttgc ttgtagcagt agtggggaat tcgtctcgag tgagtatgtt cgagttttat
    29641 tatacccaga ttatttccaa ccagagtttt cagattttga aatatctctc caggaaattg
    29701 tgtattccaa taaatttgaa catttaacgc atgctaagat tttagggaca gtcatcaatc
    29761 aattagggat tgaacggaaa ctttttggag atatcctagt agatgaagaa cgggcgcaga
    29821 ttatgattaa tcagcagttt cttcttctct ttcaagatgg actaaagaaa attggtcgta
    29881 tacctgtttc gctggaggaa cgtcctttca ccgagaaaat agataagcta gaacagtatc
    29941 gagaactgga tttatctgtg tctagttttc gattagatgt tcttttatca aatgttttga
    30001 aactatctag gaatcaagca aaccagttga ttgaaaagaa acttgtccaa gtaaattatc
    30061 atgtggtaga caaatcagat tacactgttc aagttggaga cttgattagt gtgagaaaat
    30121 ttggtcgctt gagattactt caagataagg gacaaacgaa aaaagagaag aaaaaaataa
    30181 ccgtccagtt attattaagt aagtgaggaa tagaatgcca attacatcat tagaaataaa
    30241 ggacaagact tttggaactc gattcagagg ttttgatcca gaagaagtcg atgaattttt
    30301 agatattgtg gttcgtgatt acgaagatct tgtgcgtgcg aatcatgata aaaatttgcg
    30361 tattaagagt ttagaagagc gtttgtctta ctttgatgaa ataaaagatt cattgagcca
    30421 gtctgtattg attgctcagg atacagctga gagagtgaaa caggcggcgc atgaacgttc
    30481 aaacaatatc attcatcaag cagagcaaga tgcgcaacgc ttgttggaag aagctaaata
    30541 taaggcaaac gagattcttc gtcaagcaac tgataatgct aagaaagtcg ctgttgaaac
    30601 agaagaattg aagaacaaga gccgtgtctt ccaccaacgt ctcaaatcta caattgagag
    30661 tcagttggct attgttgaat cttcagattg ggaagatatt ctccgtccaa cagctactta
    30721 tcttcaaacc agtgatgaag cctttaaaga agtggttagc gaagtacttg gagaaccgat
    30781 tccagctcca attgaagaag aaccaattga tatgacacgt cagttctctc aagcagaaat
    30841 ggaagaatta caagctcgta ttgaggtagc cgataaagaa ttgtctgaat ttgaagctca
    30901 gattaaacag gaagtggaaa ctccaactcc tgtagtgagt cctcaagttg aagaagagcc
    30961 tctgctcgtc cagttggccc aatgtatgaa gaaccagaag tagctccagt gcatccgaca
    31021 ggtccaacac cagctacaga aactgttgat tcagcaccgg gatttgaagc accgcaagaa
    31081 tctgttacaa ttttataaga aatattctga gaacaatatc ttatccttat attcccagcg
    31141 agcaggagat ggtgtgagtc ctgcattccc tatcgataag attatcctct caaaaactca
    31201 agtctgaagc tagtaagatt tgacgtttcc cacgttacgg gataagaggg agaaagacta
    31261 aaactttttc cgaataaagg tggtaccacg attttcgtcc tttttggaag tcgtggtttt
    31321 taatttgtta ttatttataa aggagatacc atgaaactca aagacaccct taatcttggg
    31381 aaaactgaat tcccaatgcg tgcaggcctt cctaccaaag agccagtttg gcaaaaggaa
    31441 tgggaagatg caaaacttta tcaacgtcgt caagaattga accaaggaaa acctcatttc
    31501 accttgcatg atggccctcc atacgctaac ggaaatatcc acgttggaca tgctatgaac
    31561 aagatttcaa aagatatcat tgttcgttct aagtctatgt caggatttta cgcaccattt
    31621 attcctggtt gggatactca tggtctgcca atcgagcaag tattgtcaaa acaaggtgtc
    31681 aaacgtaaag aaatggactt ggttgagtac ttgaaacttt gccgtgagta cgctctttct
    31741 caagtagata aacaacgtga agattttaaa cgtttgggtg tttctggtga ctgggaaaat
    31801 ccatatgtga ccttgactcc tgactatgaa gcagctcaaa ttcgtgtatt tggtgagatg
    31861 gctaataagg gttatatcta ccgtggtgcc aagccagttt actggtcatg gtcatctgag
    31921 tcagcccttg ctgaagcaga gattgaatac catgatttgg tttcaacttc cctttactat
    31981 gccaacaagg taaaagatgg caaaggagtt ctagatacag atacttatat cgttgtctgg
    32041 acaacgactc catttaccat cacagcttct cgtggtttga cggttggtgc agatattgat
    32101 tacgttttgg ttcaacctgc tggtgaagct cgtaagtttg tcgttgctgc tgaattattg
    32161 actagcttgt ctgagaaatt tggctgggct gatgttcaag ttttggaaac ttaccgtggc
    32221 caagaactca accacatcgt aacagaacac ccatgggata cagctgtaga agagttggta
    32281 attcttggtg accacgttac gactgactct ggtacaggta ttgttcatac agcccctggt
    32341 tttggtgagg acgattacaa tgttggtatt gctaataatc ttgaagtcgc agtgactgtt
    32401 gatgaacgtg gtatcatgat gaagaatgct ggtcctgaat ttgaaggtca attctatgaa
    32461 aaggtagttc caactgttat tgaaaaactt ggtaacctcc ttcttgccca agaagaaatc
    32521 tctcactcat atccatttga ctggcgtact aagaaaccaa tcatctggcg tgcagttcca
    32581 caatggtttg cctcagtttc taaattccgt caagaaatct tggacgaaat tgaaaaagtg
    32641 aaattccact cagaatgggg taaagtccgt ctttacaata tgatccgtga ccgtggtgac
    32701 tgggttatct ctcgtcaacg tgcttggggt gttccacttc ctatcttcta cgctgaagat
    32761 ggtacagcta tcatggtagc tgaaactatt gaacacgtag ctcaactttt tgaagaacat
    32821 ggttcaagca tttggtggga acgtgatgcc aaagacctct tgccagaagg atttactcat
    32881 ccaggttcac caaacggcga gttcaaaaaa gaaactgata tcatggacgt ttggtttgac
    32941 tcaggttcat catggaatgg agtggtggta aaccgtcctg aattgactta cccagccgac
    33001 ctttacctag aaggttctga ccaataccgt ggttggttta actcatcact tatcacatct
    33061 gttgccaacc atggcgtagc accttacaaa caaatcttgt cacaaggttt tgcccttgat
    33121 ggtaaaggtg agaagatgtc taaatctctt ggaaatacta ttgctccaag cgatgttgaa
    33181 aaacaattcg gtgctgaaat cttgcgtctc tgggtaacaa gtgttgactc aagcaatgac
    33241 gtgcgtatct ctatggatat cttgagccaa gtttctgaaa cttaccgtaa gattcgtaac
    33301 actcttcgtt tcttgattgc caatacatct gactttaacc cagctcaaga tacagtcgct
    33361 tacgatgagc ttcgttcagt tgataagtac atgacgattc gctttaacca gcttgtcaag
    33421 accattcgtg atgcctatgc agactttgaa ttcttgacga tctacaaggc cttggtgaac
    33481 tttatcaacg ttgacttgtc agccttctac cttgattttg ccaaagatgt tgtttacatt
    33541 gaaggtgccc aatcactgga acgccgtcaa atgcagactg tcttctatga cattcttgtc
    33601 aaaatcacca aactcttgac accaatcctt cctcacactg cggaagaaat ctggtcatat
    33661 cttgagtttg aaacagaaga cttcgtccaa ttgtcagaat taccagaagt tcaaactttt
    33721 gctaaccaag aagaaatctt ggatacatgg gcagccttca tggactttcg tggacaagca
    33781 caaaaagcct tggaagaagc tcgtaatgca aaagttatcg gtaaatcact tgaagcacac
    33841 ttgacagttt atccaaatga agttgtgaaa actctactcg aagcagtaaa cagcaatgta
    33901 gcacaacttt tgattgtgtc tgagttgacc atcgcagaag gaccagctcc agaagctgcc
    33961 cttagcttcg aagatgtagc cttcacagtt gaacgtgctg ctggtgaagt atgtgaccgt
    34021 tgccgtcgta tcgacccaac aacagcagaa cgcagctacc aggcagttat ctgtgaccac
    34081 tgtgcaagca tcgtagaaga aaactttgcg gaagcagtcg cagaaggatt tgaagagaaa
    34141 taagattgaa aagtctaggc aaaattcaat ttgagaagaa aagacaacta attttatagt
    34201 ctattaaacg cattgtatca cgtttttgaa cacctgatat gatgcgtttt ttatttattt
    34261 taaaaatttg cgaggtatga ctttttatac tcaacaagaa t
//
LOCUS       DAVHEW010000011        31439 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVHEW010000011 DAVHEW010000000
VERSION     DAVHEW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.011"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..206
                     /locus_tag="VVL53_000522"
     CDS             <1..206
                     /locus_tag="VVL53_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4182733.1"
                     /translation="EPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTFLSGKA
                     EKQGITLYHHVLIDLFISYFNPL"
     gene            complement(403..1323)
                     /gene="cysK"
                     /locus_tag="VVL53_000523"
     CDS             complement(403..1323)
                     /gene="cysK"
                     /locus_tag="VVL53_000523"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU4182734.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1572..2207)
                     /locus_tag="VVL53_000524"
     CDS             complement(1572..2207)
                     /locus_tag="VVL53_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU4182735.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2263..3561
                     /locus_tag="VVL53_000525"
     CDS             2263..3561
                     /locus_tag="VVL53_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4182736.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3558..4220
                     /locus_tag="VVL53_000526"
     CDS             3558..4220
                     /locus_tag="VVL53_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU4182737.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4300..4848
                     /gene="raiA"
                     /locus_tag="VVL53_000527"
     CDS             4300..4848
                     /gene="raiA"
                     /locus_tag="VVL53_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU4182738.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4984..6957
                     /locus_tag="VVL53_000528"
     CDS             4984..6957
                     /locus_tag="VVL53_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU4182739.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDMKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEMKEKEKEE"
     gene            6954..7406
                     /gene="rplI"
                     /locus_tag="VVL53_000529"
     CDS             6954..7406
                     /gene="rplI"
                     /locus_tag="VVL53_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU4182740.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7450..8802
                     /gene="dnaB"
                     /locus_tag="VVL53_000530"
     CDS             7450..8802
                     /gene="dnaB"
                     /locus_tag="VVL53_000530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU4182741.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8804..9070
                     /locus_tag="VVL53_000531"
     CDS             8804..9070
                     /locus_tag="VVL53_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182742.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9159..10505
                     /gene="cbpD"
                     /locus_tag="VVL53_000532"
     CDS             9159..10505
                     /gene="cbpD"
                     /locus_tag="VVL53_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU4182743.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSHSSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10588..10690
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10761..11051
                     /locus_tag="VVL53_000533"
     CDS             10761..11051
                     /locus_tag="VVL53_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU4182744.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            11014..11772
                     /locus_tag="VVL53_000534"
     CDS             11014..11772
                     /locus_tag="VVL53_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4182745.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            11811..12818
                     /locus_tag="VVL53_000535"
     CDS             11811..12818
                     /locus_tag="VVL53_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4182746.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12818..13546
                     /locus_tag="VVL53_000536"
     CDS             12818..13546
                     /locus_tag="VVL53_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182747.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13665..14123
                     /locus_tag="VVL53_000537"
     CDS             13665..14123
                     /locus_tag="VVL53_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU4182748.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            14125..16557
                     /locus_tag="VVL53_000538"
     CDS             14125..16557
                     /locus_tag="VVL53_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU4182749.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDSNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLTE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17199..17852
                     /gene="rr06"
                     /locus_tag="VVL53_000539"
     CDS             17199..17852
                     /gene="rr06"
                     /locus_tag="VVL53_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU4182750.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17849..19180
                     /gene="hk06"
                     /locus_tag="VVL53_000540"
     CDS             17849..19180
                     /gene="hk06"
                     /locus_tag="VVL53_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU4182751.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19263..19790
                     /locus_tag="VVL53_000541"
     CDS             19263..19790
                     /locus_tag="VVL53_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU4182752.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19869..21950
                     /gene="cbpA"
                     /locus_tag="VVL53_000542"
     CDS             19869..21950
                     /gene="cbpA"
                     /locus_tag="VVL53_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpA"
                     /protein_id="HEU4182753.1"
                     /translation="MFASKSERKVHYSIRKFSVGVASVVVASLVMGSVVHATENEGAT
                     QVPTSSNRANESQAEQGEQPKKLDSERDKARKEVEEYVKKIVGESYAKSTKKRHTITV
                     ALVNELNNIKNEYLNKIVESTSESQLQILMMESRSKVDEAVSKFEKDSSSSSSSDSST
                     KPEASDTAKPNKPTEPGEKVAEAKKKVEEAEKKAKDQKEEDRRNYPTITYKTLELEIA
                     ESDVEVKKAELELVKVKANEPRDEQKIKQAEAEVESKQAEATRLKKIKTDREEAEEEA
                     KRRADAKEQGKPKGRAKRGVPGELATPDKKENDAKSSDSSVGEETLPSPSLKPEKKVA
                     EAEKKVEEAKKKAEDQKEEDRRNYPTNTYKTLELEIAESDVEVKKAELELVKEEAKEP
                     RNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPA
                     PAPKAEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPA
                     QPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYY
                     LNANGSMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNANGSMATGWLQYNGSW
                     YYLNANGDMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDG
                     YGVNANGEWVN"
     gene            complement(22180..23160)
                     /gene="dusB"
                     /locus_tag="VVL53_000543"
     CDS             complement(22180..23160)
                     /gene="dusB"
                     /locus_tag="VVL53_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU4182754.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIETLLQLEKA"
     gene            complement(23147..24019)
                     /gene="hslO"
                     /locus_tag="VVL53_000544"
     CDS             complement(23147..24019)
                     /gene="hslO"
                     /locus_tag="VVL53_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU4182755.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            24184..25611
                     /locus_tag="VVL53_000545"
     CDS             24184..25611
                     /locus_tag="VVL53_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4182756.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            25770..27278
                     /gene="glpK"
                     /locus_tag="VVL53_000546"
     CDS             25770..27278
                     /gene="glpK"
                     /locus_tag="VVL53_000546"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU4182757.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQMDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            27320..29146
                     /gene="glpO"
                     /locus_tag="VVL53_000547"
     CDS             27320..29146
                     /gene="glpO"
                     /locus_tag="VVL53_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU4182758.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            29216..29920
                     /locus_tag="VVL53_000548"
     CDS             29216..29920
                     /locus_tag="VVL53_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU4182759.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            30217..30420
                     /locus_tag="VVL53_000549"
     CDS             30217..30420
                     /locus_tag="VVL53_000549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182760.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(30507..31094)
                     /locus_tag="VVL53_000550"
     CDS             complement(30507..31094)
                     /locus_tag="VVL53_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182761.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         9646 a         5545 c         7166 g         9082 t
ORIGIN      
        1 tggagcccct ttataacctc ttgcgagaga gactattgac tcagccctta cttcatgcgg
       61 atgaaacttc ttatagggtg ctagagagtg atagtcagct gacttactat tggacttttt
      121 tgtctgggaa agctgagaaa caagggatta cgctttacca tcatgttcta atcgatttgt
      181 tcatatctta tttcaatcca ttataaatag tgagaaatat ctatcctatc ttctagaatg
      241 tcttccaaac gtggaaactc tcgtaaacaa agaggtttta gaggcctatt taccatggac
      301 taaagttgta caagaaaagt gcaaataaga aatctccaga ttaggaacta tccgtgagtt
      361 ctctagtctg gagatttttc aatagacttc gttattggac ggttacaatt cataaagtgc
      421 tgtagagaga taacgttcac cgttatctgg tgctagggca aggacttttt tacctgtacc
      481 taattttttg gcaacctcga tggctccgta gatagctgca gctgaggaaa tccctacaag
      541 gaagccttct tttccaccaa tttcacgtcc gagtgcaaga gcgtcatctg atgttacacg
      601 aacgatacca tcataggctt tagtatcaag tgtatcagga ataaatccag ctgagatacc
      661 ttgaattttg tgaggaccag gtttttcacc agatagaata gcagattcat ctgcttctac
      721 tgcaaaaact tgaatgttag aattttctga tttgagtgca tgagaaacac cagaaatcgt
      781 tccaccagta cctactccag caacaaaggc atctaatcca tctttaccga aagcagctag
      841 tatctcagct cctgttgttc tttcgtgtac ttctggatta gctggattgt caaattgaag
      901 aggaaggaaa ccatcacgtt cagcagcgat ttcttgagcc ttagcaatag cacctttcat
      961 tccctcgcta ccaggagtta ggacgagttc agcaccataa gcttggataa ttttacgtcg
     1021 ttctacactc atagtttcag gcataacgat gacgacttta taccctttag cagcacctac
     1081 ccatgaaagt ccaataccgg tgtttccact tgttgcttca acaatagtag aaccaggttt
     1141 cagaatacca tcttgttcag ctttttcaat catgctaagg gcaatacggt cttttacaga
     1201 tgaaccagga ttaaatgctt caagctttat atagacgtct gcagcacctt ctggcacgat
     1261 gttgttaagt ttaacaatcg gtgtttgacc gattaattca gtaatgttgt tataaataga
     1321 catatgaata cctctttgta taagatatct ttagtataac gctatccata ccatttctat
     1381 atatcataga tcaaagtcat taattttcaa gtgattgcgt tcagctaatt ctagtaaata
     1441 ggtgtgaagg aaaacgatat caggtttcgt ttgaattgct tcattttcta gtatattttg
     1501 gtacgcctct ttttattttg ctcgctaaag tagttgtgag aatgtcaacg aaacgctgta
     1561 ttattcattg tttacactaa gtttacagga acttcaacct ctcgtaaacc ttggtcagtt
     1621 aaagtgactt ttccattaaa aaactccaca agtgcagctt taatagtttc tttttcttct
     1681 ttatcaacat aaatcatcgt atcgacttga tctgtaaagt ttgtatccag ctccatgaga
     1741 ccatgttctt taaggaagtt actgtactct tggtactgag cataagacat ttgaatagca
     1801 atgccagcct gttcttttat ttcaataata ccaatttctt tgacagctaa ggcgacactg
     1861 ccggcgtaag cacgaattag tcctccagcg cctagtttaa taccaccaaa gtagcgtgtc
     1921 acgaccacac agacattggt gagattgtga ttttctagta ccccaagcat gggaacacca
     1981 gcagtaccac taggctcacc atcatcactt gtacgtttaa tttcactacg ttctccaata
     2041 atgaaggcag agcagttatg tgtcgctttg tagtgttctt ttttgatggc agtaatgaag
     2101 tcacgagcct cttcttcgct ataaacacgc ttggcatggc agataaagcg agattttttg
     2161 atttcttctt ggacttgacc gtcctcttta attgtcctaa attccatgat ttaattgtac
     2221 taaaaaatca tctaaagtgc tagtttttac gaataaagaa gtatgaaagt aaatttagat
     2281 tatctcggtc gtttatttac tgagaatgaa ttaacagaag aagaacgtca gttggcggag
     2341 aaacttccag caatgagaaa ggagaagggg aaacttttct gtcaacgctg taatagtact
     2401 attctagaag aatggtattt gcccatcggt gcttactatt gtcgagagtg cttgctgatg
     2461 aagcgagtca gaagtgatca aactttatac tattttccgc aggaggattt tccaaagcaa
     2521 gatgttctca aatggcgcgg ccaattaact ccttttcaag agaaggtgtc agagggattg
     2581 cttcaagtag tagacaagca aaagccaacc ttagttcatg cggtaacagg agctggaaag
     2641 acagaaatga tttatcaagt agtggctaaa gtgatcaatg cgggtggtgc agtgtgtttg
     2701 gctagtcctc gcatagatgt ttgtttggag ctgtacaagc gcctgcaaca ggatttttct
     2761 tgcgggatag ctttgctaca tggagaatcg gaaccttatt ttcgaacacc actagttgtt
     2821 gcaacaaccc atcagttatt gaagttttat caagcttttg atttgctgat agtggatgaa
     2881 gtagatgctt ttccttatgt tgataatccc atgctttacc acgctgtcaa gaatagtgta
     2941 aaggagaatg gattgagaat ctttttaaca gcgacttcga ccaatgagtt agataaaaag
     3001 gtccgtttag gagaactaaa aagactgaat ttaccgagac ggtttcatgg aaatccgttg
     3061 attattccaa aaccaatttg gttatcggat tttaatcgct acttagacaa gaatcgtttg
     3121 tcaccaaagt taaagtccta tattgagaag cagagaaaga cagcttatcc gttactcatt
     3181 tttgcttcag aaattaagaa aggggagcag ttagcagaaa tcttacagga gcaatttcca
     3241 aatgagaaaa ttggctttgt atcttctgta acagaggatc gattagagca agtacaagct
     3301 tttcgagatg gagaactgac aatacttatc agtacgacaa tcttggagcg cggagttacc
     3361 ttcccttgtg tggatgtttt cgtagtagag gccaatcatc gtttgtttac caagtctagt
     3421 ttgattcaga ttggtggacg agttggacga agcatggata gaccgacagg agatttgctt
     3481 ttcttccatg atgggttaaa tgcttcaatc aagaaggcga ttaaggaaat tcagatgatg
     3541 aataaggagg ctggtctatg aagtgcttgt tatgtgggca gactatgaag actgttttaa
     3601 cttttagtag tctcttactt ctgaggaatg atgactcttg tctttgttca gactgtgatt
     3661 ctacttttga aagaattggg gaagagaact gtccaaattg tatgaaaaca gagttgtcaa
     3721 caaagtgtca agattgtcaa ctttggtgta aagagggagt tgaagtcagt catagagcga
     3781 tttttactta caatcaagct atgaaggatt ttttcagtcg gtataagttt gatggagact
     3841 tcctgttaag aaaagttttc gcttcatttt taagtgagga gttgaaaaag tacaaagagt
     3901 atcaatttgt tgtaattccc ctaagtcctg atagatatgc taatagagga tttaatcagg
     3961 ttgagggctt ggtagaggca gcaggctttg agtatctgga tttattagag aaaagagaag
     4021 agagagccag ttcttctaaa aatcgttcag agcgcttggg gacagaactt cctttcttta
     4081 ttaaaagtgg agtcactatt cctaaaaaaa tcctacttat agatgatatc tatactacag
     4141 gagcaactat aaatcgtgtt aagaaactgt tggaagaagc tggtgctaag gatgtaaaaa
     4201 cattttccct tgtaagatga ggaaaaaatt tgcaaaaata gaatgaaagc gttataataa
     4261 aatcataaaa acaaaaatgt ttagaaagaa ggtactcata tgattaaata tagtatccgt
     4321 ggtgaaaacc tagaagtaac agaagcaatt cgtgattatg tagtttctaa actcgaaaag
     4381 atcgaaaagt acttccaacc agaacaagag ttggatgccc gaattaactt aaaagtttat
     4441 cgtgaaaaaa cggctaaagt ggaagtaacg attccgcttg gatctattac tctccgcgca
     4501 gaagatgtat ctcaagatat gtatggttca attgaccttg taactgataa aattgaacgt
     4561 cagattcgta aaaataaaac aaaaatcgag cgtaaaaata aaaataaggt agcaactggt
     4621 caattattta cagatgcttt ggtggaagat tcaaatattg tccagtctaa agttgttcgt
     4681 tcaaaacaaa ttgatttaaa accaatggat ttggaagaag caattctaca aatggattta
     4741 ttggggcatg atttctttat ctatgtggat gttgaagatc agacaaccaa tgtgatttat
     4801 cgtcgtgagg atggcgaaat tggtttgtta gaggttaaag aatcttaatt tcaatatgtg
     4861 taaaggtagg tttactgaat tgtaaactac ctttttctta taattgatag aattactgat
     4921 tacactttta aaaagtcgct ttttgagggg tattatggta taatgggtgc caagaggttt
     4981 tgaatgaaaa aattttatgt aagtccaatt tttcctattc tagtaggatt gattgcgttt
     5041 ggagtcttat ccactttcat tatttttgtt aataataatc tgttgacggt tttaattttg
     5101 tttctttttg taggaggcta tgttttttta tttaagaaac tgagagtgca ttatacaagg
     5161 agtgatgtag aacagataca gtatgtaaac caccaagcgg aagaaagttt gacagctcta
     5221 ttggaacaga tgcctgtagg tgttatgaaa ttgaatttat cttctggaga ggttgagtgg
     5281 tttaatccct atgctgaatt gattttgacc aaggaagatg gtgattttga tttagaagct
     5341 gttcaaacga ttatcaaggc ttcagtagga aatccgtcta cttatgccaa gcttggtgag
     5401 aagcgttatg ctgttcatat ggatgcttct tccggtgttt tgtattttgt agatgtatcc
     5461 agggaacaag ccataacaga tgaattggta acaagtagac cagtgattgg gattgtctct
     5521 gtggataatt atgatgattt ggaggatgaa acttctgagt cagatattag tcaaatcaat
     5581 agttttgtag ctaattttat atcagagttt tcagaaaaac acatgatgtt ttctcgtcgg
     5641 gtaagtatgg atcgatttta tctatttact gactacacgg tgcttgaggg cttgatgaat
     5701 gataaatttt ctgttattga tgctttcaga gaagagtcga aacagagaca gttgcccttg
     5761 accttaagta tgggattttc ttatggcgat ggaaatcatg atgagatagg gaaagttgct
     5821 ttgctcaatt tgaacttggc tgaagtacgt ggtggcgacc aggtggttgt taaggaaaac
     5881 gacgaaacga aaaatccagt ttattttggt ggtgggtctg ctgcttcaat caagcgtaca
     5941 cggactcgta cgcgcgctat gatgacagct atttcagata agattcggag tgtagatcag
     6001 gtttttgtag tcggtcacaa aaatttagac atggatgctt tgggctctgc tgtaggtatg
     6061 cagttgttcg ccagcaatgt gattgaaaat agctatgctc tttatgatga agaacaaatg
     6121 tctccagata ttgaacgagc tgtttcattc atagaaaaag aaggagttac gaagttgttg
     6181 tctgttaagg atgcaatggg gatggtgacc aatcgttctt tgttgattct tgtagaccat
     6241 tcaaagacag ccttaacatt atcaaaagaa ttttatgatt tatttaccca aaccattgtt
     6301 attgaccacc atagaaggga tcaggatttt ccagataatg cggttattac ttatatcgaa
     6361 agtggtgcaa gtagtgccag tgagttggta acggaattga ttcagttcca gaattctaag
     6421 aaaaatcgtt tgagtcgtat gcaagcaagt gtcttgatgg ctggtatgat gttggatact
     6481 aaaaatttca cctcgcgagt aactagtcgg acatttgatg ttgctagcta tctcagaacg
     6541 cgcggaagtg atagtattgc tatccaggaa atcgctgcga cagattttga agaatatcgt
     6601 gaggtcaatg aacttatttt acaggggcgt aaattaggtt cagatgtact aatagcagag
     6661 gctaaggaca tgaaatgcta tgatacagtt gttattagta aggcagcaga tgccatgtta
     6721 gccatgtcag gtattgaagc gagttttgtt cttgcgaaga atacacaagg atttatctct
     6781 atctcagctc gaagtcgtag taaactgaat gtacaacgga ttatggaaga gttaggcggt
     6841 ggaggccact ttaatttggc agcagctcaa attaaagatg taaccttgtc agaagcaggt
     6901 gaaaaactga cagaaattgt attaaatgaa atgaaggaaa aggagaaaga agaatgaaag
     6961 taatcttttt agcagatgtt aaaggaaaag gtaaaaaagg cgaaattaag gaagtaccaa
     7021 cagggtatgc gcaaaacttt cttatcaaaa agaatctagc caaagaagcg actgctcaag
     7081 ctgtaggtga acttcgtggt aaacaaaaat cggaagaaaa agctcacgct gagatgattg
     7141 cagaaggaaa agcaattaaa gcacaacttg aagcagaaga aactgttgta gaatttgttg
     7201 aaaaagttgg tccagatggt cgtacctttg gttctattac caataagaag attgcagaag
     7261 aattgcaaaa gcaatttgga attaagattg ataaacgtca tattcaagta caagctccga
     7321 ttcgagcggt tggtttgatt gatgtgccag tgaaaatcta tcaagatatc acaagtgtaa
     7381 tcaatcttcg tgtgaaagaa gggtaagttt acaccttctt gacaagattg taaaaggaag
     7441 ggaagtctga tggcagaagt agaagagtta cgagtacaac ctcaagatat cttagctgag
     7501 caatccgttt taggggctat ctttattgat gagagtaaac ttgtttttgt gcgagaatac
     7561 attgagtctc gggacttttt taagtatgcc catcgtttga ttttccaagc catggtcgat
     7621 ttatccgatc gtggtgatgc catagatgca acaacggttc gtactatcct tgataatcaa
     7681 ggtgatttac agaatattgg tggcttgtct tacttggttg agattgttaa ttctgtgcca
     7741 acttctgcta atgcggagta ttatgctaag attgttgcag aaaaagcaat gctacgtcgt
     7801 ttaattgcca agttgacaga gtctgtcaac caagcttacg aagcgtcaca accagctgat
     7861 gaaattattg ctcaggcaga aaaagggttg attgatgtca gtgaaaatgc aaatcgaagc
     7921 gggtttaaga acattcgaga tgtgttgaat ctcaactttg gaaatctgga agctcgctcg
     7981 caacaaacga ccgatattac aggtattgcg acaggttatc gtgatttgga tcatatgaca
     8041 acaggacttc atgaggagga gttgattatc ttagcagctc gtccagcagt tggtaagaca
     8101 gcatttgcct tgaatatcgc tcagaacatt gggactaagt tggacaaaac ggttgctatt
     8161 ttttcactcg aaatgggtgc ggaaagcttg gtagatcgta tgttagctgc agaaggctta
     8221 gtggagtcac attctatccg tacagggcaa ttgacagatg aggagtggca aaaatatact
     8281 attgctcagg gtaatctagc taacgccagt atctatatcg atgatacgcc aggtattcgg
     8341 attacagaga ttcgttctcg ttctcgtaaa ttggctcaag aaactggaaa tcttggtttg
     8401 attttgatag actatttgca acttatcacg ggaactggtc gagaaaatcg tcaacaagaa
     8461 gtttctgaaa tttctcgtca gttgaaaata ctagccaagg aattgaaggt tccagtaatc
     8521 gctctgagtc agctttctcg tggtgtagaa caacgtcagg acaagagacc ggtcttgtct
     8581 gatattcgtg aatctgggtc tattgagcag gacgctgata tcgtagcttt tctctatcgc
     8641 gatgactact atgaacgtgg tggtgaagaa gaggagggta tcccaaataa taaggtggaa
     8701 gttattatcg agaaaaaccg tagtggagct cgtggaacag tggaattgat tttccaaaaa
     8761 gaatacaata aattttcaag tatctcaaag agggaggcat aagatgtcag atgcatttac
     8821 agatgtagcc aagatgaaaa aaatcaaaga agaaatcaag gcacatgagg gacaagtcgt
     8881 agaaatgact ttggagaatg gtcgtaagcg ccaaaaaaat agattgggta agctaattga
     8941 agtttatcca tctctattta ttgtggagtt tggggatgtg gaaggagata aacaagttaa
     9001 tgtttacgtt gaatccttta cttactcaga tattcttaca gaaaagaatt tgattcatta
     9061 tcttgactaa agtgagaaat tttctcactt tttctttttt ctccgaataa tttaggtgaa
     9121 ggcaatcatc gctttatatt atttttcaag gaggaagaat gaaaatttta ccgtttatag
     9181 caagaggaac aagttattac ttgaagatgt cagttaaaaa gcttgttcct tttttagtag
     9241 taggattgat gctagcagct ggtgatagtg tctatgccta ttccagagga aatggatcga
     9301 ttgcgcgtgg ggatgattat cctgcttatt ataaaaatgg gagccaggag attgatcagt
     9361 ggcgcatgta ttctcgtcag tgtacttctt ttgtagcctt tcgtttgagt aatgtcaatg
     9421 gttttgaaat tccggcagct tatggaaatg cgaatgaatg gggacatcgt gctcgtcggg
     9481 aaggttatcg tgtagataat acaccgacga ttggttccat tacttggtct actgcaggaa
     9541 cttatggtca tgttgcctgg gtgtcaaatg taatgggaga tcagattgag attgaggaat
     9601 ataactatgg ttatacagaa tcctataata aacgagttat aaaagcaaac acgatgacag
     9661 gatttattca ttttaaagat ttggatggtg gcagtgttgg gaatagtcaa tcctcaactt
     9721 caacaggcgg aactcattat tttaagacca agtctgctat taaaactgaa cctctagcta
     9781 gcggaactgt gattgattac tattatcctg gggagaaggt tcattatgat cagatacttg
     9841 aaaaagacgg ctataagtgg ttgagttata ctgcctataa tggaagctat cgttatgttc
     9901 aattggaggc tgtgaataaa aatcctctag gtaattctgt tctttcttca acaggtggaa
     9961 ctcattattt taagaccaag tctgctatca aaactgaacc cctagttagt gcaactgtga
    10021 ttgattacta ttatcctgga gagaaggttc attatgatca aattctcgaa aaagacggct
    10081 acaagtggtt gagttatacg gcttataacg gaagtcgtcg ctatatacag ctagagggag
    10141 tgacttcttc acaaaattat cagaatcaat caggaaacat ctctagctat ggatcccata
    10201 gtagttcaac tgtcggttgg aagaaaataa atggtagttg gtatcatttc aaatcaaatg
    10261 gttctaaatc aacaggatgg ctgaaagacg gttctagctg gtattatttg aaattatctg
    10321 gtgaaatgca gacaggatgg ttaaaggaaa atggtttgtg gtattatctg ggtagttcag
    10381 gggcaatgaa aacaggctgg taccaggtct ctggtaagtg gtattattct tactcttcag
    10441 gcgccttagc tgttaatacg acggtggatg gctacagagt aaacagtgat ggagaacgag
    10501 tatagaaaat tggagtagga gaaatttcct gctcttttct gtaagcagtt tccttgacat
    10561 ttttcttatt ttgcgctatc atatatacat ataatatatg ggagtctgtg tacagtctga
    10621 gaggaagtgt taaacttcga ccgcacctga tctgggtaat gccagcggag ggaacgatac
    10681 ttagtctaat tttgcacctt ttccatgtat ggtaaaggtt tttctttttt taaaaaggaa
    10741 aacgagaaga ggaggttctt atgaaagcaa gcattgcctt gcaagtttta cccctagtac
    10801 aggggattga tcggatagct gttattgatc aggtcattgc ttatctgcaa actcaagaag
    10861 tgacgatggt agtgacacca tttgaaacgg tcttggaagg ggagtttgat gagcttatgc
    10921 gcattctaaa agaagcgctg gaagtggcag ggcaggaggc agacaatgtc tttgccaatg
    10981 tcaaaataaa tgtaggagag attttaagta ttgatgagaa acttgagaag tatactgaga
    11041 cgacacatta gtctattggg ctttctcgga gtattgtcaa tctggcagtt agcaggtttt
    11101 cttaaacttc tccccaagtt tatcctgccg acacctcttg aaattctcca gccctttgtt
    11161 cgtgacagag aatttctctg gcaccatagc tgggcgacct tgagagtggc tttactgggg
    11221 ctgattttgg gagttttgat tgcctgtctt atggctgtgc tcatggatag tttgacttgg
    11281 ctcaatgacc tgatttaccc tatgatggtg gtcattcaga ccattccgac cattgccata
    11341 gctcctatcc tggtcttgtg gctaggttat gggattttgc ccaagattgt cttgattatc
    11401 ttaacgacaa cctttcccat catcgttagt attttggacg gttttaggca ttgcgacaag
    11461 gatatgctga ccttgtttag tctgatgcgg gccaagcctt ggcaaatcct gtggcatttt
    11521 aaaatcccag ttagcctgcc ttacttttat gcaggtctga gggtcagtgt ctcctacgcc
    11581 tttatcacaa ctgtggtatc tgagtggttg ggaggttttg aaggtcttgg tgtttatatg
    11641 attcagtcta aaaaactgtt tcagtatgat accatgtttg ccattattat tctggtgtcg
    11701 attatcagtc ttttgggtat gaagctggtc gatatcagtg aaaaatatgt gattaaatgg
    11761 aaacgttcgt agaattagaa tgtttctgaa aaagaaaaga ggaaatcaaa atgaagaaaa
    11821 catggaaagt gtttttaacg cttgtaacag ctcttgtagc tgttgtgctt gtggcctgtg
    11881 gtcaaggaac tgcttctaaa gacaacaaag aggcagaact taagaaggtt gactttatcc
    11941 tagactggac accaaatacc aaccacacag ggctttatgt tgccaaggaa aaaggttatt
    12001 tcaaagaagc tggagtggat gttgatttga aattgccacc agaagaaagt tcttctgact
    12061 tggttatcaa cggaaaggca ccatttgcag tgtatttcca agactacatg gctaagaaat
    12121 tggaaaaagg agcaggaatc actgccgttg cagctattgt tgaacacaat acatcaggaa
    12181 tcatctctcg taaatctgat aatgtaagca gtccaaaaga cttggttggt aagaaatatg
    12241 ggacatggaa tgacccaact gaacttgcta tgttgaaaac cttggtagaa tctcaaggtg
    12301 gagactttga gaaggttgaa aaagtaccaa ataacgactc aaactcaatc acaccgattg
    12361 ccaatggcgt ctttgatact gcttggattt actacggttg ggatggtatc cttgctaaat
    12421 ctcaaggtgt agatgctaac ttcatgtact tgaaagacta tgtcaaggag tttgactact
    12481 attcaccagt tatcatcgca aacaacgact atctgaaaga taacaaagaa gaagctcgca
    12541 aagtcatcca agccatcaaa aaaggctacc aatatgccat ggaacatcca gaagaagctg
    12601 cagatattct catcaagaat gcacctgaac tcaaggaaaa acgtgacttt gtcatcgaat
    12661 ctcaaaaata cttgtcaaaa gaatacgcaa gcgacaagga aaaatggggt caatttgacg
    12721 cagctcgctg gaatgctttc tacaaatggg ataaagaaaa tggtatcctt aaagaagact
    12781 tgacagacaa aggcttcacc aacgaatttg tgaaataatg acagaaatta gactagagca
    12841 cgtcagttat gcctatggtc aggagaggat tttagaggat atcaacctac aggtgacttc
    12901 aggcgaagtg gtttccatcc taggcccaag tggtgttgga aagaccaccc tctttaatct
    12961 aatcgctggg attttagaag ttcagtcagg gagaattgtc cttgatggtg aagaaaatcc
    13021 caaggggcgc gtgagttata tgttgcaaaa ggatctgctc ttggagcaca agacggtgct
    13081 tggaaatatc attctgcccc tcttgattca aaaggtggat aaggcagaag ctatttcccg
    13141 agcggataaa attcttgcga ccttccagct gacagctgta agagacaagt atcctcatga
    13201 acttagcggt gggatgcgcc agcgtgtagc cttactccgg acctaccttt ttgggcacaa
    13261 gctctttctc ttagatgagg cctttagcgc cttggatgag atgacaaaga tggaactcca
    13321 cgcttggtat cttgagattc acaagcagtt gcagctaaca accctgatca tcacgcatag
    13381 tattgaggag gccctcaatc tcagcgaccg tatctatatc ttgaaaaatc gccctgggca
    13441 gattgtttca gaaattaaac tagattggtc tgaagatgag gacaaggaag tccaaaagat
    13501 tgcctacaaa cgtcaaattt tggcggaatt aggcttagat aagtagaaaa atagggagtt
    13561 ggtgaagatt atcctttacc agcgcccttt ttcttttaaa aatgagaaaa tttcggtata
    13621 atagtcaaac aaggtcaagg tttaaagaga gaggtgggtt tgttatgaga tttaaaaata
    13681 catcggatca tattgaggcc tacatcaagg cgattttaga tcaatctggt atcgtggagt
    13741 tgcaacggag tcagttggca gatacctttc aggttgttcc tagtcagatt aactacgtga
    13801 tcaagacacg ctttacggaa agtagaggct acttggttga aagtaagcgt ggtggcggag
    13861 gctacattcg tataggacgg attgagtttt ctagtcatca tgaaatgctc cgggagctgc
    13921 tttactcgat tggtgagcga gtcagtcaag aaatttatga ggatattctc cagcttttgg
    13981 ttgagcagga attgatgacc aagcaggaga tgaatttgct agaatcagta gctttggatc
    14041 gcgttttagg agaagaagct ccagttgttc gagcaaacat gctacgtcag atcatacaag
    14101 aggtagatag aaaagggaag taagatgaac tattcaaaag cattgaatga atgtatcgaa
    14161 agtgcctaca tggttgctgg acattttgga gctcgttatc tagagtcgtg gcacttgttg
    14221 attgccatgt ctaatcacag ttatagtgta gcaggggcaa ctttaaatga ttatccgtat
    14281 gagatggacc gtttagaaga ggtggctttg gaactgactg aaacggacta tagccaggat
    14341 gaaaccttta cggaattgcc gttctcccgt cgtttgcagg ttctttttga tgaagcagag
    14401 tatgtagcgt cagtggtcca tgctaaggta ctagggacag agcacgtcct ctatgcgatt
    14461 ttgcatgata gcaatgcctt ggcgactcgt atcttggaga gggctggttt ttcttatgaa
    14521 gacaagaaag atcaggtcaa gattgctgct cttcgtcgaa atttagaaga acgggcaggc
    14581 tggactcgtg aagatctcaa ggctttacgc caacgccatc gtacagtagc tgacaagcaa
    14641 aattctatgg ccaatatgat gggcatgccg cagactccta gtggtggtct cgaggattat
    14701 acgcatgatt tgacagagca agcgcgttct ggcaagttag aaccagtcat cggtcgggac
    14761 aaggaaatct cacgtatgat tcaaatcttg agccggaaga ctaagaacaa ccctgtcttg
    14821 gttggggatg ctggtgtcgg gaaaacagct ctggcgcttg gtcttgccca gcgtattgct
    14881 agtggtgacg tgcctgcgga aatggctaag atgcgcgtgt tagaacttga tttgatgaat
    14941 gtcgttgcag ggacacgctt ccgtggtgac tttgaagaac gcatgaataa tatcatcaag
    15001 gatattgaag aagatggcca agtcatcctc tttatcgatg aactccacac catcatgggt
    15061 tctggtagcg ggattgattc gactctggat gcggccaata tcttgaaacc agccttggcg
    15121 cgtggaactt tgagaacggt tggtgccact actcaggaag aatatcaaaa acatatcgaa
    15181 aaagatgcgg cactttctcg tcgtttcgct aaagtgacga ttgaagaacc aagtgtggca
    15241 gatagtatga ctattttaca aggtttgaag gcgacttatg agaaacatca ccgtgtacaa
    15301 atcacagatg aagcggttga aacagcggtt aagatggctc atcgttattt aaccagtcgt
    15361 cacttgccag actctgctat cgatctcttg gatgaggcgg cagcaacagt gcaaaataag
    15421 gcaaagcatg taaaagcaga cgattcagat ttgagtccag ctgacaaggc cctgatggat
    15481 ggcaagtgga aacaggcagc ccagctaatc gcaaaagaag aggaagtacc tgtctacaaa
    15541 gacttggtga cagagtctga tattttgacc accttgagtc gcttgtcagg aatcccagtt
    15601 caaaaactga ctcaaacgga tgctaagaag tatttaaatc ttgaagcaga actccataaa
    15661 cgggttatcg gtcaagatca agctgtttca agcattagcc gtgccattcg ccgcaaccag
    15721 tcagggattc gcagtcataa gcgtccgatt ggttccttta tgttcctagg gcctacaggt
    15781 gtcgggaaaa ctgaattagc caaggctctg gcagaagttc tttttgacga cgaatcagcc
    15841 cttatccgct ttgatatgag tgagtatatg gagaaatttg cagctagtcg tctcaacgga
    15901 gctcctccag gctatgtagg atatgaagaa ggtggggagt tgacagagaa ggttcgcaat
    15961 aaaccctatt ccgttctcct ctttgatgag gtagagaagg cccacccaga tatctttaat
    16021 gttctcttgc aggttctgga tgacggtgtc ttgacagata gcaagggacg caaggtcgat
    16081 ttttcaaata ccattatcat tatgacatcg aatctaggtg cgactgccct tcgtgatgat
    16141 aagactgttg gttttggggc taaggatatt cgttttgacc aggaaaatat ggaaaaacgc
    16201 atgtttgaag aactgaaaaa agcttataga ccggaattca tcaaccgtat tgatgagaag
    16261 gtggtcttcc atagcctatc tagtgatcat atgcaggaag tggtgaagat tatggtcaag
    16321 cctttagtgg caagtttgac tgaaaaaggc attgacttga aattacaagc ttcagctctg
    16381 aaattgttag caaatcaagg atatgaccca gagatgggag ctcgcccact tcgcagaacc
    16441 ctgcaaacag aagtggagga caagttggca gaacttcttc tcaagggaga tttagtggca
    16501 ggcagcacac ttaagattgg tgtcaaagca ggccagttaa aatttgatat tgcataaaag
    16561 aataaaagta tcagcatctg accataagtc acagtggagt gaaattcaat gaaaatcaaa
    16621 gagcaaacta ggcagctagc cgcaggttgc tcaaaacact ggtttgaggt tgcagataga
    16681 gctgacgtgg tttgaagaga ttttcgaaga gtatgaaact aaaacctata gcttctaaac
    16741 gatccgtggt tttcatcatt caacacaaaa ttcatatgtt tattaccctc cgtcgtattt
    16801 gtcttagagc gtgtgtagta gaaaaagagc agtcttatct gaaattttta ttctttcaaa
    16861 agagacctgt ttcttttttg catgtcaaat ccgttctagc tggtatttga aaaatcaaac
    16921 taatattcaa tgaaaatcaa agaacaaact aggaagctag ccgcaggttg ctcaaaacac
    16981 tgttttgagg ttgtagatag agctgacgtg gtttgaagag attttcgaag agtataagct
    17041 gcaagatgaa tgattttctt gtattgacgt tgttgttgac aaaaagtagc ggataaatga
    17101 aatccattcc attatcatag atgataggct ggtaggaaat tttcaaatag catacaggaa
    17161 atagatgtat ggagttctgg tagtagaaag ggagagagat gaacatttta gttgcagatg
    17221 acgaggaaat gattagagaa ggaattgcag catttctgac agaagagggt tatcatgtca
    17281 ttatggctaa ggatggacaa gaggtcttgg aaaaatttca agatctccct atccatctca
    17341 tggtactgga tttaatgatg cctaggaaga gtggttttga agtgttaaaa gaaatcaatc
    17401 aaaagcacga tattcctgtc atcgtcttga gtgctctggg agatgaaact actcagtcac
    17461 aggtatttga tctctatgct gatgatcatg tgacaaaacc tttttctttg gtactgcttg
    17521 tcaagcgtat taaggcgctt atcagacgtt actacgtcat agaggatctt tggcgatatc
    17581 aggatgtaac agtggatttt acctcttaca aagcacatta taaaaatgaa gaaattgatc
    17641 tcaaaccaaa ggaattactg gtactaaagt gtttgattca gcataaaaat caagttttaa
    17701 gtagagagca gatattggaa gaaatttcaa aagatgtagc tgatttacct tgtgataggg
    17761 tcgttgatgt ctatattcgt actcttcgca aaaaattagc tttagattgt atcgtgactg
    17821 tgaaaaatgt tgggtataag attagcttat gataaaaaat cctaaattat taaccaagtc
    17881 ttttttaaga agttttgcaa ttctaggtgg tgttggtcta gtcattcata tagctattta
    17941 tttgaccttt cctttttatt atattcaact ggagggggaa aagtttaatg agagcgcaag
    18001 agtgtttacg gagtatttaa agactaagac atctgatgaa attccaagct tactccagtc
    18061 ttattcaaag tccttgacca tatctgctca ccttaaaaga gatattgtag ataagcggct
    18121 ccctcttgtg catgacttgg atattaaaga tggaaagcta tcaaattata tcgtgatgtt
    18181 agatatgtct gttagtacag cagatggtaa acaggtaacc gtgcaatttg ttcacggggt
    18241 ggatgtctac aaagaagcaa agaatatttt gcttttgtat ctcccatata catttttggt
    18301 tacaattgct ttttcctttg ttttttctta tttttatact aaacgcttgc tcaatcctct
    18361 tttttacatt tcagaagtga ctagtaaaat gcaagatttg gatgacaata ttcgttttga
    18421 tgaaagtagg aaagatgaag ttggtgaagt tggaaaacag attaatggta tgtatgagca
    18481 cttgttgaag gttatttatg agttggaaag tcgtaatgag caaattgtaa aattgcaaaa
    18541 tcaaaaggtt tcctttgtcc gcggagcatc acatgagttg aaaacccctt tagccagtct
    18601 tagaattatc ctagagaata tgcagcataa tattggagat tacaaagatc atccaaaata
    18661 tattgcaaag agtataaata agattgacca gatgagccac ttattagaag aagtactgga
    18721 gtcttctaaa ttccaagagt ggacagagtg tcgtgagacc ttgactgtta agccagtttt
    18781 agtagatatt ttatcacgtt atcaagaatt agctcattca ataggtgtta caattgaaaa
    18841 tcaattgaca gatgctacca gggtcgtcat gagtcttagg gcattggata aggttttgac
    18901 aaacctgatt agtaatgcaa ttaaatattc agataaaaat gggcgtgtaa tcatatccga
    18961 gcaagatggc tatctctcta tcaaaaatac atgtgcgcct ctaagtgacc aagaactaga
    19021 acatttattt gatatattct atcattctca aatcgtgaca gataaggatg aaagttccgg
    19081 tttgggtctt tacattgtga ataatatttt agaaagctat caaatggatt atagttttct
    19141 cccttatgaa cacggtatgg aatttaagat tagcttgtag acagattagt tttttattaa
    19201 agttcatata gggttaacat aagtgtgtta ttctttgtgt agataaaaga aaggatacta
    19261 atatggtatt agcgattatt ttagtaacat tctttattcg attgattttt ttaaagcgtt
    19321 cgatagagaa tgagaaacga atccttagca atggcgggaa agaatttgga gttgagaata
    19381 caaaacgatt aactatggct catattgttt tttatctctc ttgcttggtt gaggcaatgg
    19441 tgcacaagac aatttttgat ggcatgggca tggttggttt agtcttgctt attttttcta
    19501 tgctgatgtt gatgttggtg attcacttgt tgggagatat ttggacagtg aagcttatgc
    19561 ttgtcaataa tcacaaatat gtagatcata tcttgtttag gacagtaaaa caccctaatt
    19621 actttttaaa tattcttcct gagttgattg gcttgacctt gttgagtcat gcttatgtga
    19681 cttttgtttt agtttttcca gtttatgcag ttattttgta tcgacgaata gctgaagagg
    19741 aaaagctatt acatgaagtt ataatcccaa atggaagcat aaagagataa atacaaaatt
    19801 cgatttatat acagttcata ttgaagtgat atagtaaggt taaagaaaaa atatagaagg
    19861 aaataaacat gtttgcatca aaaagcgaaa gaaaagtaca ttattcaatt cgtaaattta
    19921 gtgttggagt agctagtgta gttgttgcca gtcttgttat gggaagtgtg gttcatgcga
    19981 cagagaacga gggagctacc caagtaccca cttcttctaa tagggcaaat gaaagtcagg
    20041 cagaacaagg agaacaacct aaaaaactcg attcagaacg agataaggca aggaaagagg
    20101 tcgaggaata tgtaaaaaaa atagtgggtg agagctatgc aaaatcaact aaaaagcgac
    20161 atacaattac tgtagctcta gttaacgagt tgaacaacat taagaacgag tatttgaata
    20221 aaatagttga atcaacctca gaaagccaac tacagatact gatgatggag agtcgatcaa
    20281 aagtagatga agctgtgtct aagtttgaaa aggactcatc ttcttcgtca agttcagact
    20341 cttccactaa accggaagct tcagatacag cgaagccaaa caagccgaca gaaccaggag
    20401 aaaaggtagc agaagctaag aagaaggttg aagaagctga gaaaaaagcc aaggatcaaa
    20461 aagaagaaga tcgtcgtaac tacccaacca ttacttacaa aacgcttgaa cttgaaattg
    20521 ctgagtccga tgtggaagtt aaaaaagcgg agcttgaact agtaaaagtg aaagctaacg
    20581 aacctcgaga cgagcaaaaa attaagcaag cagaagcgga agttgagagt aaacaagctg
    20641 aggctacaag gttaaaaaaa atcaagacag atcgtgaaga agcagaagaa gaagctaaac
    20701 gaagagcaga tgctaaagag caaggtaaac caaaggggcg ggcaaaacga ggagttcctg
    20761 gagagctagc aacacctgat aaaaaagaaa atgatgcgaa gtcttcagat tctagcgtag
    20821 gtgaagaaac tcttccaagc ccatccctga aaccagaaaa aaaggtagca gaagctgaga
    20881 agaaggttga agaagctaag aaaaaagccg aggatcaaaa agaagaagat cgccgtaact
    20941 acccaaccaa tacttacaaa acgcttgaac ttgaaattgc tgagtccgat gtggaagtta
    21001 aaaaagcgga gcttgaacta gtaaaagagg aagctaagga acctcgaaac gaggaaaaag
    21061 ttaagcaagc aaaagcggaa gttgagagta aaaaagctga ggctacaagg ttagaaaaaa
    21121 tcaagacaga tcgtaaaaaa gcagaagaag aagctaaacg aaaagcagca gaagaagata
    21181 aagttaaaga aaaaccagct gaacaaccac aaccagcgcc ggctccaaaa gcagaaaaac
    21241 cagctccagc tccaaaacca gagaatccag ctgaacaacc aaaagcagaa aaaccagctg
    21301 atcaacaagc tgaagaagac tatgctcgta gatcagaaga agaatataat cgcttgactc
    21361 aacagcaacc gccaaaaact gaaaaaccag cacaaccatc tactccaaaa acaggctgga
    21421 aacaagaaaa cggtatgtgg tacttctaca atactgatgg ttcaatggcg acaggatggc
    21481 tccaaaacaa tggctcatgg tactacctca acagcaatgg cgctatggcg acaggatggc
    21541 tccaaaacaa tggttcatgg tactatctaa acgctaatgg ttcaatggca acaggatggc
    21601 tccaaaacaa tggttcatgg tactacctaa acgctaatgg ttcaatggcg acaggatggc
    21661 tccaatacaa tggctcatgg tactacctaa acgctaatgg ttcaatggcg acaggatggc
    21721 tccaatacaa tggctcatgg tactacctaa acgctaatgg tgatatggcg acaggttggg
    21781 tgaaagatgg agatacctgg tactatcttg aagcatcagg tgctatgaaa gcaagccaat
    21841 ggttcaaagt atcagataaa tggtactatg tcaatggctc aggtgccctt gcagtcaaca
    21901 caactgtaga tggctatgga gtcaatgcca atggtgaatg ggtaaactaa acctaatata
    21961 actagttaat actgacttcc tgtaagaact ctttaaagta ttccctacaa ataccatatc
    22021 ctttcagtag ataatatacc cttgtaggaa gtttagatta aaaaataact ctgtaatctc
    22081 tagccggatt tatagcgcta gagactacgg agtttttttg atgaggaaag aatggcggca
    22141 ttcaagaggc tctttaagag agttacgggt tttaaactat taagccttct ccaattgcaa
    22201 gagggtttca atctctgcca gggtgctggc ttgcgaaatg gctccacgga gtttggcagc
    22261 gccagatgtt ccacggagat agtgaggagc gagaccgcgg aattcacgaa ctgcgacgtt
    22321 ttctcctttg aggttaatca atcgtttcaa gtgttcgtag gcgatcttca tcttgtcttc
    22381 aaaggtcaaa tcaggtagga tttctcctgt ttcaaagtaa tggttgattt ggttgaagag
    22441 gtaaggattt cccatggcag ctcggccaat catgactgcg tcagcaccaa cttcttcgat
    22501 gcgttgcttg gcttcttgga cagtacggat atcaccgttg gcgatgaatg gaatcttggt
    22561 tagagcttgg gcaaccttgt aaagggtctc aaggtctgcg tggccagtat acatttgttc
    22621 acgggtacgg ccatgcatgg cgagggcaga aacacctgca gcttcagcag cgagagcatt
    22681 ttctactgca agagatgggt ccgcccagcc ggtacgcatt ttgacagtaa gtgggatatc
    22741 aaggacagac tggaccttgt tgatgatgga gtaaatcttg tctggatcct tgagccacat
    22801 agcaccagct tcgttcttca cgattttgtt gacagggcag cccatgttga tatcgacgat
    22861 atcggtcttg gtgttttctt ggatgaattc tgctgcgcgt gctaggctgt cttcatcgct
    22921 accaaaaagt tggatagaga cagggttttc gccctcatcg atatgaagca tgtgcagggt
    22981 tttttcgttg ttgtattgga ttcccttgtc agagaccatt tccattacaa cgagtccagc
    23041 tccgagctcc tttgcgatag tacgaaaggc tgagttggtc acgccagcca taggcgctaa
    23101 aacggtacga ttgggaatct caatattgcc aatcataaaa ggtgtattaa gatttgtcac
    23161 gaatgagttc ctccaggtcc ttttcatcaa agttgtaagt agtttggcag aattgacaag
    23221 tgatttctgc cccgtggtct tcctctttca tttcctgtaa gtctgagctt ggaaggctgg
    23281 caagagcgtt cataaagcgt tcatggctac agtcacattg gaaacggatt tcttcttcag
    23341 aaagacgctt gtaggcttcg tccccgtaga tagccttgag gagggcttcg atatggtcgt
    23401 cgctttcgag aagagtagag atagctggca tttcttggat gcgtttttca aagcgagcaa
    23461 tctcttcttt cttggctcct ggcaagactt gaactaggaa accacctgca accttgacct
    23521 tgtcttcctc gtccaaaagg acattgaggc cgaccgctga aggcgtttgt tggctttcag
    23581 taaggtaaaa ggcaaggtct tcaccgattt ctccagagat gaggggagtt atagagttgt
    23641 aaggatttcc agtaccgtag tctgtgataa cgaggaattg accatttcca acaaaaggtc
    23701 cgactaggac ttcaccagtc gcagtctttt tgatgtcaac accaggattt tgaacatagc
    23761 ctttgacgtt ccccttggta tcagcgacgg tgataatagc acctagagag ctagatccca
    23821 acaccttaac tgtaagtttg gtatttcctt tttcattggc tgcgagaatc tggctagcga
    23881 taagagttcg accaagcgct acagttgagc tagcttgggt ttgatgtttt tcttgagcag
    23941 tgcggacggt ttcagtgcta tcaaggacaa aagcacgaaa ggctccgctt tctgatatag
    24001 ttttaataat tttatccata gctactattt tagcataaaa atgcccaaag ggggagccgt
    24061 gtgtttactg attttcagga taatggacca ggaaatcagc atgaaaataa aaagagaaac
    24121 agattatttt agcatttgtc agatttatgc tatgcttaag gtagaaaatg aaagggataa
    24181 caaatgtatt taggagattt gatggagaaa gccgagtgtg gtcaattttc aatcctttcc
    24241 tttctattac aagagtctca gacgaccgtc aaggctgtaa tggaagaaac aggattttca
    24301 aaagcaaccc taaccaaata tgtcaccctg ctcaatgaca aggctttgga tagtggctta
    24361 gagctggcta ttcactcaga agatgaaaat ctgcgtctgt ctatcggtgc agctaccaag
    24421 gggagagata ttcggagctt gtttttggag agtgctgtta aataccagat tttggtttat
    24481 cttctctacc accaacagtt tttagcccat cagctggctc aagaattggt gattagcgag
    24541 gctacgcttg gtcgtcactt ggctggttta aatcagattt tgtcagaatt tgatttatcc
    24601 atccaaaatg gccgttggcg aggtccagag catcagattc gctatttcta tttctgtctt
    24661 ttccgaaagg tctggtcgag tcaggaatgg gaaggtcaca tgcagaaacc agagagaaaa
    24721 caggagattg ccaatttaga ggaaatctgc ggtgcaagtt tgtctgcggg gcagaaattg
    24781 gacttggttc tctgggctca catcagtcaa caacgtcttc gggtcaatgc ttgtcagttt
    24841 caagtcatag aagagaaaat gcgagggtat tttgacaata tcttttatct tcgtttgctg
    24901 agaaaggttc cgtccttttt tgctgggcaa catattccac taggagttga ggatggtgag
    24961 atgatgatat tcttctcttt tctcctatct catcgcattc ttcctcttca tactatggag
    25021 tatattcttg gttttggagg gcagttggca gatttactga cgcaattgat tcaagaaatg
    25081 aagaaggagg aactattggg ggattataca gaggaccatg tcacctatga actcagtcag
    25141 ctttgtgctc aagtctatct ctataagggc tatattttac aggatcgcta caagtaccag
    25201 ttagagaatc gtcatccata tttactgatg gaacatgatt ttaaagagac agcagaggag
    25261 atttttcatg ctctacctgc ttttcaacag gggacagatt tagataagaa gattctctgg
    25321 gaatggctcc agttaatcga atatatggct gaaaacggtg gccagcatat gcggattggt
    25381 ctggatttga catctggttt tcttgtcttt tcaaggatgg cagccatttt gaaacggtat
    25441 ttggaataca atcgttttat taccattgaa gcttatgacc ctagtcggca ttatgatttg
    25501 ctggttacca ataacccgat tcataagaag gaacagacac cagtctatta tttaaaaaat
    25561 gacttggata tggaggattt ggtagcgatt cgccagttat tattcactta aaaggcttgg
    25621 ttaatccagg tcttttttgt gaaattcaca caatctcctc acattttttt aaaaattaaa
    25681 aaaagttgat aaacaagaaa gcgctttatt ttgtatacta gtaagtgtaa agaggaaaca
    25741 cctcaagatc tttatcagga ggacagtaca tgtcacaaga aaaatacatc atggccattg
    25801 accagggaac tacaagttct cgtgccatca ttttcaacaa aaaaggggaa aaggttagct
    25861 cgagtcaaaa agagtttacc cagattttcc ctcaggcagg ttgggttgag cacaatgcca
    25921 atgaaatttg gaactctgtt cagtcagtta ttgcgggtgc tttcatcgaa agtggtgtca
    25981 agccaaatca aatcgaggca atcgggatta ccaaccaacg tgaaacaacg gttgtctggg
    26041 ataagaaaac aggacttcct atctacaatg ctatcgtttg gcagtcacgc cagacagcac
    26101 ctttggctga gcaactaaaa agccaaggtt atgtggaaaa attccatgaa aagactggtt
    26161 tgattattga tgcttacttc tctgctacca aggttcgttg gattttggat catgtagaag
    26221 gtgctcaaga gcgagcagaa aaaggggaat tgctctttgg tactatcgat acttggttgg
    26281 tttggaaatt gactgacggt gcggctcacg tgactgacta ctcaaatgca gctcgtacca
    26341 tgctttataa cattaaagaa ctcaaatggg atgatgagat tttggaaatc cttaacattc
    26401 cgaaggctat acttccagaa gttcgttcta actccgaaat ctacggcaag acagctccat
    26461 tccatttcta cggtggagag gtgccaatct caggtatggc tggggaccaa caagcagccc
    26521 tctttggaca gttggctttt gagccaggta tggttaagaa tacttatgga acaggctctt
    26581 tcatcatcat gaatactggg gaagagatgc agttgtctga aaacaacctc ttgacaacca
    26641 ttggttacgg aatcaacggt aaggtttatt atgccttgga aggttctatc ttcatcgcag
    26701 gaagtgctat tcagtggctt cgtgacggtc ttcgcatggt tgaaaattca ccagaatctg
    26761 aaaaatacgc tcgtgattct cacaacaacg atgaagttta tgtcgttcca gcctttacag
    26821 gtctaggcgc tccatactgg aaccaaaatg ctcgtggttc cgtctttggt ttgactcgtg
    26881 gaacaagcaa agaagacttt atcaaggcga ctttgcaatc tattgcttat caagtgcgtg
    26941 acatcatcga caccatgcaa atggatactc agaccgccat tcaagtactg aaggtggatg
    27001 gtggtgcagc catgaacaac ttcctcatgc agttccaggc ggatatttta ggcattgaca
    27061 ttgcacgtgc taaaaacttg gaaacaacag ctctaggagc ggccttccta gcaggtttgt
    27121 cagtagggta ctggaaagac ttggacgagt tgaaactctt gaacgagaca ggagaactct
    27181 ttgagccatc tatgaatgaa tctcgcaagg aacaactcta caagggctgg aagaaggctg
    27241 tgaaagcaac tcaagtcttt gcggaagtag acgactaata ctggcagaat aaagcgattt
    27301 atttagaaag tgtgtaaata tggaattttc aaagaaaaca cgtgaattgt caattaaaaa
    27361 aatgcaggaa cgcaccctgg acctcttgat tatcggtgga ggaatcacag gagctggtgt
    27421 agccttgcag gcggcagcta gcggtcttga gactggtttg attgaaatgc aagactttgc
    27481 agaaggaaca tctagtcgtt caacaaaatt ggttcacgga ggacttcgtt acctcaaaca
    27541 atttgacgta gaggtggtct cagatacggt ttctgaacgt gcagtggttc aacaaatcgc
    27601 tccacacatt ccaaaaccag atccaatgct cttaccagtt tacgatgaag atggagcaac
    27661 ctttagcctc ttccgtctta aagtagccat ggacttgtac gaccttttag caggtgtgag
    27721 caacacacca actgcgaaca aggttttgag caaggatcaa gtcttggaac gccagccaaa
    27781 cttgaagaag gaaggcttgg taggaggtgg agtgtatctt gacttccgta acaacgatgc
    27841 gcgtctcgtg attgaaaaca tcaaacgtgc caaccaagac ggtgccctca ttgccaacca
    27901 cgtgaaggca gaaggcttcc tctttgacga aagtggcaag attacaggtg ttgtagctcg
    27961 tgatctcttg acagaccaag tatttgaaat caaggcccgt ctggttatta atacaacagg
    28021 tccttggagt gataaagtac gtaatttgtc taataaggga acgcaattct cacaaatgcg
    28081 cccaactaag ggagttcact tggtagtgga ttcaagcaaa atcaaggttt cacagccagt
    28141 ttactttgac acaggtttgg gtgacggtcg tatggtcttt gttctcccac gtgaaaacaa
    28201 gacttacttt ggtacaactg atacagacta cacaggtgat ttggagcatc caaaagtaac
    28261 tcaagaagat gtagattatc tacttggcat tgtcaacaac cgcttcccag aatccaacat
    28321 caccattgat gatatcgaaa gcagctgggc aggtcttcgt ccattgattg cagggaacag
    28381 tgcctctgac tataatggtg gaaataacgg taccatcagt gatgaaagct ttgacaactt
    28441 gattgcgact gttgaatctt atctctccaa agaaaaaaca cgtgaagatg ttgagtctgc
    28501 tgtcagcaag cttgaaagta gcacatctga gaaacatttg gatccatctg cagtttctcg
    28561 tgggtctagc ttggaccgtg atgacaatgg tctcttgact cttgctggtg gtaaaatcac
    28621 agactaccgt aagatggctg aaggagctat ggagcgcgtg gttgacatcc tcaaagcaga
    28681 atttgaccgt agctttaaat tgatcaattc taaaacttac cctgtttcag gtggagaatt
    28741 gaacccagca aatgtggatt cagaaatcga agcctttgcg caacttggag tatcacgtgg
    28801 tttggatagc aaggaagctc actatctggc aaatctttac ggttcaaatg caccgaaagt
    28861 ctttgcactt gctcacagct tggaacaagc gccaggactc agcttggcag atactttgtc
    28921 ccttcactat gcaatgcgca atgagttgac tcttagccca gttgacttcc ttcttcgtcg
    28981 taccaatcac atgctcttta tgcgtgatag cttggatagt atcgttgagc caattttgga
    29041 tgaaatggga cgattctatg actggacaga agaagaaaaa gcaacttacc gtgctgatgt
    29101 cgaagcagct ctcgctaaca acgatttagc agaattaaaa aattaagaaa aaataaaaga
    29161 ggtggagggc agcattcctt gtcgcccgtc ccttcttttt aatggagaca gaaagatgat
    29221 gaatgaatta tttggagaat ttctagggac tttaatcctg attcttctag gaaatggtgt
    29281 tgttgcaggt gtggttcttc ctaaaaccaa gagcaatagc tcaggttgga ttgtgattac
    29341 tatgggttgg gggattgcag ttgcggttgc agtctttgta tctggcaagc tcagtccagc
    29401 ttatttaaac ccagctgtga ccatcggtgt ggccttaaaa ggtggtttgc cttgggcttc
    29461 cgttttgcct tatatcttag cccagttcgc aggggccatg ctgggtcaga ttttggtttg
    29521 gttgcaattc aaacctcact atgaggcaga agaaaatgca ggcaatatcc tggcaacctt
    29581 cagtactgga ccagccatca aggatactgt atcaaacttg attagcgaaa tccttggaac
    29641 ttttgttttg gtgttgacaa tctttgcttt gggtctttac gactttcagg caggtatcgg
    29701 aacctttgca gtgggaactt tgattgtcgg tatcggtcta tcactaggtg ggacaacagg
    29761 ttatgccttg aacccagctc gtgaccttgg acctcgtatc atgcacagca tcttgccaat
    29821 tccaaacaag ggagacggag actggtctta cgcttggatt cctgttgtag gccctgttat
    29881 cggagcagcc ttggcagtgc ttgtattctc acttttctag tttatactct tcgaaaatca
    29941 aattcaaacc acgtcagcgt cgccttaccg tactcaagta cagcttgcgg ctagcttcct
    30001 agtttgctct ttgattttca ttgagtatta gaaaccaatt atgttgatag agcttgggca
    30061 agagcccaat ttcagcaaaa aatgaagtaa atcttctcat aataaaacgc atcatatcaa
    30121 gcacgaaaat tccacgaggt caactacagt cagaaagctg aacaacaagc caaaacgccc
    30181 aaaaaaggcg gcaaaaagca agcacctgca agcaacgtgc cgaaatggtc aaatcctgat
    30241 tatgtcaacg aattagaccc aaaaatcgtt gatatgctag tagaatttca caagtcacaa
    30301 ggcactttgg aaactcccga ggcgcaagca gaaatcgccc aaaaacgtga agaaatcgag
    30361 caaaggagag ctgagcttga gggtaaaaaa caagagcttt tgaaccgctt gaacaaatag
    30421 agtttcgcaa gtattatgct tacaaattac ttgagcaatt aactaaaata taaaccctgc
    30481 ctttatatct aggcagggtt tatattttag aaattcacgt aggttgttac ggtttttaca
    30541 tacccagtat agtttgagtt tctatagtat tcagtgataa acttccattt tctttgagca
    30601 acatggatat aagtacttgt tatgtagtat ggatatgggc tttgtgaatc caagtaagac
    30661 tgataagctt gtataccaaa atatgctcca ccaattattg caccccatgg accccccaat
    30721 aaagcaccta tcctaccaat catataactg attccagcac cagtcatgaa gttagcgaat
    30781 gtgttagctt gtttattccc atgtattgtg ttgacgtaat tccaaacatt aggatcgtat
    30841 gatctaaaag atatatttag gtcgatttca ttcttttgat aagccatata aaatgcccca
    30901 ttgatataga cgccgtcagc acgtcgttca atagtgtcta cacttccatc tggattgaca
    30961 acctcaagaa cttcatcgct taaaatattt acttgcgtat ctccgaaccg cactgatgag
    31021 ccattctcaa actgagcctc accagataca actttagagt ttgccgataa gctatcatca
    31081 gcaaaaacaa acaagcgacg gggaaatgct agacatacag aaaacagaca taactagcaa
    31141 acacatgcat ttaaacatct tagacataac ggaaactcct ttgtattttt gatttttttc
    31201 aacttttatt atacaataaa accaaataaa aagaaagcgg taacaatatg cttaatgcga
    31261 aaatttttta tatattttta tgtttgatcg ttatcgaaac tacaggcttg ttgttgttga
    31321 aaagaggtct cgaaatgggt tatttagaca cagaagctat tatcctcgca gttttttcat
    31381 ttgcttttta caacctatgt tcattcgctt gggtctgctc tacaataaaa aacaataaa
//
LOCUS       DAVHEW010000012        30141 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVHEW010000012 DAVHEW010000000
VERSION     DAVHEW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.012"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            163..597
                     /locus_tag="VVL53_000551"
     CDS             163..597
                     /locus_tag="VVL53_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4182762.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            609..1799
                     /locus_tag="VVL53_000552"
     CDS             609..1799
                     /locus_tag="VVL53_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU4182763.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDYVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1810..2301
                     /locus_tag="VVL53_000553"
     CDS             1810..2301
                     /locus_tag="VVL53_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU4182764.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVTIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2316..3095
                     /locus_tag="VVL53_000554"
     CDS             2316..3095
                     /locus_tag="VVL53_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU4182765.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3082..3900
                     /locus_tag="VVL53_000555"
     CDS             3082..3900
                     /locus_tag="VVL53_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4182766.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3900..4193
                     /gene="yajC"
                     /locus_tag="VVL53_000556"
     CDS             3900..4193
                     /gene="yajC"
                     /locus_tag="VVL53_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU4182767.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIHVEKESVMGKTKELLFK"
     gene            4215..6116
                     /locus_tag="VVL53_000557"
     CDS             4215..6116
                     /locus_tag="VVL53_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU4182768.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMNLEEAYHFASKELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNQEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGIHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6176..7177
                     /locus_tag="VVL53_000558"
     CDS             6176..7177
                     /locus_tag="VVL53_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4182769.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7287..7622)
                     /locus_tag="VVL53_000559"
     CDS             complement(7287..7622)
                     /locus_tag="VVL53_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182770.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7633..7875)
                     /locus_tag="VVL53_000560"
     CDS             complement(7633..7875)
                     /locus_tag="VVL53_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182771.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7886..8428)
                     /locus_tag="VVL53_000561"
     CDS             complement(7886..8428)
                     /locus_tag="VVL53_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU4182772.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(8444..8638)
                     /locus_tag="VVL53_000562"
     CDS             complement(8444..8638)
                     /locus_tag="VVL53_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4182773.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8804..9754
                     /gene="rsmH"
                     /locus_tag="VVL53_000563"
     CDS             8804..9754
                     /gene="rsmH"
                     /locus_tag="VVL53_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU4182774.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLARIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9766..10083
                     /gene="ftsL"
                     /locus_tag="VVL53_000564"
     CDS             9766..10083
                     /gene="ftsL"
                     /locus_tag="VVL53_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU4182775.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            10087..12339
                     /gene="pbpX"
                     /locus_tag="VVL53_000565"
     CDS             10087..12339
                     /gene="pbpX"
                     /locus_tag="VVL53_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU4182776.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQISQQTVDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITKDFVWRDILYQSNYE
                     PGSAMKVMTLAASIDNNTFPGGEYFNSSELKIADVTIRDWDVNEGLTGGGMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     NYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KSLEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFEGSGTTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            12341..13321
                     /locus_tag="VVL53_000566"
     CDS             12341..13321
                     /locus_tag="VVL53_000566"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU4182777.1"
                     /translation="MFISISAGIVTFLLTVVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLSSLLTLAILYLM"
     gene            13533..13673
                     /locus_tag="VVL53_000567"
     CDS             13533..13673
                     /locus_tag="VVL53_000567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182778.1"
                     /translation="MADRTDEVSSKHGFEVVDKEKLMWFEEVFEECKKYWSVKVKTLD
                     LF"
     gene            13929..16034
                     /locus_tag="VVL53_000568"
     CDS             13929..16034
                     /locus_tag="VVL53_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU4182779.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYIIEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
     gene            complement(16330..16812)
                     /locus_tag="VVL53_000569"
     CDS             complement(16330..16812)
                     /locus_tag="VVL53_000569"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU4182780.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(16907..18391)
                     /locus_tag="VVL53_000570"
     CDS             complement(16907..18391)
                     /locus_tag="VVL53_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU4182781.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            18544..20151
                     /locus_tag="VVL53_000571"
     CDS             18544..20151
                     /locus_tag="VVL53_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU4182782.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLNSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPAIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTDQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            20316..20483
                     /locus_tag="VVL53_000572"
     CDS             20316..20483
                     /locus_tag="VVL53_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU4182783.1"
                     /translation="MKKRILSAGLTFASALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(20476..20997)
                     /locus_tag="VVL53_000573"
                     /pseudo
     CDS             complement(20476..20997)
                     /locus_tag="VVL53_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            21220..22665
                     /locus_tag="VVL53_000574"
     CDS             21220..22665
                     /locus_tag="VVL53_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU4182784.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVYFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDMLEGR"
     gene            22667..23398
                     /gene="cps4B"
                     /locus_tag="VVL53_000575"
     CDS             22667..23398
                     /gene="cps4B"
                     /locus_tag="VVL53_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU4182785.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            23407..24099
                     /gene="cpsC"
                     /locus_tag="VVL53_000576"
     CDS             23407..24099
                     /gene="cpsC"
                     /locus_tag="VVL53_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU4182786.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            24109..24798
                     /locus_tag="VVL53_000577"
     CDS             24109..24798
                     /locus_tag="VVL53_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU4182787.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTPPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            24813..26174
                     /locus_tag="VVL53_000578"
     CDS             24813..26174
                     /locus_tag="VVL53_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4182788.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRIIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            26180..26923
                     /locus_tag="VVL53_000579"
     CDS             26180..26923
                     /locus_tag="VVL53_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU4182789.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            26920..27744
                     /locus_tag="VVL53_000580"
     CDS             26920..27744
                     /locus_tag="VVL53_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU4182790.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            27746..28624
                     /locus_tag="VVL53_000581"
     CDS             27746..28624
                     /locus_tag="VVL53_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4182791.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            28625..29959
                     /locus_tag="VVL53_000582"
     CDS             28625..29959
                     /locus_tag="VVL53_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU4182792.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPSLNTKIKVTDRLIDIQFWKIAL
                     TIIVDLVILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
BASE COUNT         9207 a         5238 c         6646 g         9050 t
ORIGIN      
        1 cctaaaaaaa cggaagattt tgaaatttct gtctattaaa ctggttaaaa agataatgaa
       61 agcgctaata tttttttaaa aaaactattg acaagataaa aataaaggat tacaatgggg
      121 aatataaagt aaaccggtaa acctaaaaag aaaggagaaa agatgaaaat tgtacttgta
      181 gggcatggac attttgctac agggatttat agttctttac aattgattgc aggtaatcaa
      241 gaaaatgtgg aggcgattga ctttgtggaa ggaatgtcag cagatgaact caagcaaaaa
      301 atcttacttg caatttcaaa tgaagaagaa gttttaatcc taagtgatct cttgggagga
      361 tcgccattca aggtttcttc taccataatg ggagaaaatc cagccaagac aatgaatgtt
      421 ctctcgggtt tgaacttagc catgttaatg gaagcagtct ttgctagaat ggctcatagc
      481 tttgatgagg ttgttaataa atcagtagtg gcggcccagg gcggagtcgt aaatggtaaa
      541 gaattgtttt caacggatgc agaggaagag gaagaagatt tcgaatcggg tatttaaagg
      601 gtaaaagaat gataaaaaag gttacgattg aaaaaataaa atcgcctgag cgcttcttag
      661 aagtaccact tctgacgaaa gaagaagtcg gccaggcaat cgataaggtt attcggcagt
      721 tagaactcaa ccttgactat ttcaaggaag atttcccgac gccagctacc tttgataatg
      781 tctatccaat catggataac acggaatgga ccaatggttt ctggacagga gaactgtggt
      841 tggcttatga atacagtcaa caggatgcat ttaaaaacat cgctcataaa aatgttcttt
      901 ctttcctgga tcgtgtcaat aagagagtag aattggatca ccatgatctc ggcttcttgt
      961 acacaccgtc ttgtatggct gaatataaga taaatggaga tggagaggct agagaagcaa
     1021 ccttgaaagc tgcagataag ttgattgaac gctatcaaga aaaaggtggt tttattcaag
     1081 cttggggaga cttgggcaag aaagagcatt accgtttgat tatcgactgc ttgctcaata
     1141 tccaactctt attctttgct tatcaagaaa caggcgatca aaaatactac gatattgcag
     1201 aaagccattt ctatgcttca gctaataatg taatccgtga tgacgcttcg tccttccaca
     1261 ccttctattt tgatcctgag acaggtcaac cctttaaagg tgtaacgaga caagggtata
     1321 gtgatgattc atgctgggca cgtggtcaat catggggagt ctatggtatt cctttgactt
     1381 atcgtcactt aaaagacgag tcctgctttg acttgtttaa gggtgtgacc aattatttct
     1441 tgaatcgtct gccaaaagat tatgtgtcct attgggattt gatttttaat gatggtagtg
     1501 atcaatcacg agattcttca gcaacagcta tcgccgtctg tgggattcat gaaatgctaa
     1561 aacatctccc agaggtggat gctgacaaag atatttataa acatgctatg catgccatgc
     1621 ttcgttcctt gatcgaacat tatgcaaatg atcaatttac ccctggtggg acaagtctcc
     1681 tccacggtgt gtactcatgg cattcaggta aaggagtgga tgaaggcaat atctggggtg
     1741 actactatta cctagaagcc cttatccgtt tctacaaaga ctggaaccta tattggtagg
     1801 aggagaaata tgacaatgcc aaatattatt atgacccgta tcgatgaacg gttgattcat
     1861 ggacaaggac aactttgggt aaaataccta ggttgtaata cggtcattgt tgccaatgac
     1921 gaagtaagca cggacaagat gcaacaaact ctgatgaaaa cagttgtgcc agactcagtt
     1981 gccatgcgtt tcttcccttt gcaaaaggtg attgatatca ttcacaaggc taatcctgct
     2041 caaacgatct ttatcgttgt aaaggatgtg aaggacgctt taaccttggt agaaggtggt
     2101 gtcactatca aagaaatcaa tattgggaac attcacaatg cccctggtaa agagcaagtg
     2161 acacgctcca tcttcctggg tgaagaggac aaggcggccc tcaaggaatt gagccaaact
     2221 catcaagtaa catttaatac gaaaacaact ccaacaggaa atgatggagc tgttcaagtc
     2281 aacattatgg actatattta aaagaggaga tcgttatgtc gattaatgta tttcaagcga
     2341 ttttaattgg attatggaca gctttctgtt ttagtggaat gctgttagga atttacacca
     2401 atagatgtat tgttctgtca tttggtgtcg gaattattct aggtgatctg cctactgctc
     2461 ttgcaatggg agctattggt gaattggctt atatgggatt cggtgttggt gctggaggta
     2521 ctgttccacc aaacccaatc ggacctggta tctttggtac cttgatggct atcactagtg
     2581 ctggtaaagt cagtccagaa gcggctcttg ccctctctac tccgattgct gtggcgattc
     2641 aattcttaca aactttcgcc tacactgtac gtgctggtgc gcctgaaaca gctatgaagc
     2701 acttgaaaaa ccataatttg aagaaattta agttcactct aaatgcaaca atttggttgt
     2761 ttgcctttat tggatttacc ttgggttgct tgggtgccct ttcaatggat accttgttga
     2821 aactcgtaga ctacattcca ccggtattac ttacaggttt gacagttgct ggtaaaatgc
     2881 tcccagctat cggttttgcg atgatcttgt cagtgatggc taagaaagag ttgattccct
     2941 ttgtcttgtt gggatatgtt tgtgcagctt atctaaacat cccaacaatt ggtattgcaa
     3001 ttgtaggtac tatctttgct ttgattgaat tttataacaa gccaaaaaca gcggatcatg
     3061 tggtagagga ggaagcacac gatgactgga tctaacaaat taacaaaacg tgattatctt
     3121 aaaacgtctt tgcgggcatt cttttgtcaa aatggattta actatagtaa ctatcaaggg
     3181 ttgggatatg ccaatgtgat gtatcctgct ttgaaaaaac actatggaga ggatcaggaa
     3241 ggtttctacc aagccttgga agaaaactgt gaattctata ataccaaccc acacttcctg
     3301 ccttttatta ccagcttgca tcttgtaatg ttggaaaatg gccgtccggc aaaagaaaca
     3361 cgtagcatca agatggcctt gatgggacca ttggcaggta ttggggattc tctttctcaa
     3421 ttctgtttag ctccattgtt ctcaaccatc gcagcttcgt ttgctcaaga aggcttggtt
     3481 gtcggtccaa tcttgttctt ccttgcgatg aatacgattt taacagcgat taaattgtca
     3541 actggtctgt atggatacaa actaggaaca actgtgattg ataaactaag cgaacagatg
     3601 gcaacgattt ctcgtattgc caatattatc ggtgtaaccg taattgctgg tttggcagcg
     3661 acatctgtta agattatggt gccgattacc tttgctgcag gggaagttaa agcagacgct
     3721 aaacaaagta tcgtaagtat tcagggaatg cttgataagg ttgctccagc tcttctacca
     3781 gccctattta cacttttagt ttactacttg atcaaagaaa agaaatggac aacatataaa
     3841 ctcgttattt taacagttat catcggaatt atcggaagct ggcttaagat tatagcttaa
     3901 tggatacaac attgttttat ggaatagtga ttgtcttggc agtgagtccc cttttactgt
     3961 caagctttca ttctattcgt cagcaaaagt tgcttcgcaa acagatggag caacgacaag
     4021 agtatttagc ttctttaaca tctggtgatg aagtgttgtt attgtctgga attcatggaa
     4081 aaatcatttc tatccaagat gacttggtct ccttgcagat tgcaaaaggt gtggtcatcc
     4141 atgtagaaaa ggaaagtgta atgggaaaga caaaagaact gctttttaag tagttctttt
     4201 tctggtaata gataatgaaa gaagaaagaa gacaattttt tgaaagaatc gatggaaacc
     4261 aatgtcgtga ttatatcttg tcgcactgtt caaaagacta tgagaaagtc aagagttccc
     4321 ttgaacgctt gatggacaat cgttttatgt ttgatagtcc ttgggatatg gagccttgtt
     4381 caaaaatcca tcaaatccaa ccgatggtat gggatcaagt atttgaagat gatccagaat
     4441 gggcttatat gctcaatcga caagaatatc tcttgcagtt tatgataggg tatctggtag
     4501 aaggagataa ggactatatt caaaagtgca agttctttct atttgattgg attgagcagg
     4561 tgagagaatt ttctcctcaa tccttgatga ctagaacctt ggatacgggt attcgttcct
     4621 ttacttggtt gaaactactc ttgctcctct tgaaatttga cttgctagag gagaaagaac
     4681 tagagaaaat tttggtcagt ctagaaaagc agattgactt tatgaaaagc tactatcgcg
     4741 ccaagtacac ccttagtaac tgggggattt tacaaacaat tccgatgctt gctatctatc
     4801 atttcttttc agataagatg aacctagaag aagcttacca ttttgcttca aaggagttga
     4861 aacagcaaat tgagacacag attttaggag atggaagcca gtttgaacag tcgattctct
     4921 atcatgtaga ggtttataaa gccttgctgg atttgtgtct cttgcttcca gacttgcaag
     4981 atagttacca agagttgctg gaaaagatgg cgacctatat tcaaatgatg acaggcttag
     5041 atggacggac tttggctttt ggtgatagcg attctacaga aacgacagaa atgttgagcc
     5101 tgtctgctgt ggttttgaac caggaagacc ttcttaacgg tctggatgtt aaagttgatt
     5161 tgcttagcct cttgttcctg gggcgagaaa aggtcaagcg actgcaggaa tttgaaagga
     5221 gagcttggca gcctaagtcc atgatctttg aagactctgg acatgtctgc attaaggatg
     5281 aacatcgtta tctatttttc aaaaatggtc cgctaggaag tgcccatagc catagtgacg
     5341 agaatagttt ttgcttacag tatcaaggac aacctatttt catagatgct gggcgttatt
     5401 cttatcggga gatatatgag cgttatctct taaagagtgc ttggagtcat tcgacctgca
     5461 ttgtagatgg gaaagctccg gaaagaatca cgggatcctg ggaatatgaa tactatcctc
     5521 actccctgtt ttgtcaccat aaagaaaggg agggaataca ttatattgag ggggcttatt
     5581 ggtcagcaga acctgatttg ccttatcttc acaagagaaa aatcctcatg ttggtagagg
     5641 atgtctggct cttggtagat gacatcaggt gtcaaggtca gcatgaggcg ttgactcagt
     5701 ttatccttga caaggatgtg acctatcaag atgggaaaat caatcagttg agactatgga
     5761 gtgaagttga ttttgatttg gaagatacca tcatttctcc taaatacaat gagcttgaaa
     5821 gaagtagcaa actcaccaag cgccaattct ttgagaatca gatgctggat tataccatca
     5881 ttgcgcatga gagttttgaa atcatccgtc attctgtcta ccagacagat gatcgtgaag
     5941 tggaaaatgc tctggctttt gaagtgaaaa atgacgaaac agacaagctg attctgttat
     6001 taagcgagga tattggtgta ggtgaaaaat tgtgcctcgt tgacggaaca aaaatgcgtg
     6061 gaaaatgtct agtatatgat aaaataaatg agagaatgat tcgcttgcag tgctagaaat
     6121 aggcattttg aatagtgaat atgttataat aagtattagt aggaggtgtt ttagattgga
     6181 gaagaaactg accataaaag acattgcgga aatggctcag acctcgaaaa caaccgtgtc
     6241 attttaccta aacgggaaat atgaaaaaat gtcccaagag acacgtgaaa agattgaaaa
     6301 agttattcat gaaacaaatt acaaaccgag cattgttgcg cgtagcttaa actccaaacg
     6361 aacaaaatta atcggtgttt tgatcggtga tattaccaac agtttctcaa accaaattgt
     6421 taagggaatt gaggatatcg ccagccagaa tggctaccag gtaatgatag gaaatagtaa
     6481 ttacagccaa gagagtgagg accggtatat tgaaagcatg cttctcttgg gagtagacgg
     6541 ctttattatt cagccgacct ctaatttccg aaaatattct cgtatcatcg atgagaaaaa
     6601 gaagaaaatg gtcttttttg atagtcagct ctatgaacac cggactagct gggttaaaac
     6661 caataactat gatgccgttt atgacatgac ccagtcctgt atcgaaaaag gttatgagca
     6721 ttttctcttg attacagcgg atacgagtcg tttgagtact cggattgagc gggcaagtgg
     6781 ttttgtggat gctttaacag atgctaatat gcgtcacgcc agtctaacca ttgaagataa
     6841 gcatacaaat ttggaacaaa ttaaagaatt tttacaaaaa gaaatcgatc ccgatgaaaa
     6901 aactctggta tttatcccta actgttgggc cctacctcta gtctttaccg ttatcaaaga
     6961 gttgaattat aacttgccgc aagttgggtt gattggtttt gacaatacgg agtggacttg
     7021 cttttcttct ccaagtgttt cgacgctggt tcagccctcc tttgaggaag ggcaacaggc
     7081 tacaaagatt ttgattgacc agattgaagg ccgcaatcaa gaagaaaggc aacaagtctt
     7141 ggattgtagt gtgaattgga aagagtcgac tttctaaaat gaaggaaaat gacttgcaat
     7201 ctctgttaag aaataaaata atcccaccta gaacaagcta ggtgggatta tttgcctatg
     7261 aaatgagaaa ttatgggagc aagctcctaa atcaactgtt tttgatctac ttctttaact
     7321 acttgataaa agttatagaa gtaggccaaa cttgaaatga tggttacgac taggaatatt
     7381 gaaaatttcc attggacagg gttggttaaa agttgtggaa aggatatgag gagaaagaag
     7441 agggctgcgt tgaggacagg tatccgtttt gattgtattt tctcaagtcc tttattgagc
     7501 gcaggaagaa agaggagtag gagtagtaaa actgtatgag aaacagctcc tgaaataagg
     7561 gcgaagaaaa ggaaaatact gataaaaaca tgaatgatca gtagtctagc tagtgatttc
     7621 ataaggcacc tcctaatcct ggtctttttt agctcttgca atacgaagtg agtcgacaat
     7681 atgtatcatc actccgaaaa agaaagctcc cagtatagtt ttaaaaatat gttttgtatt
     7741 tagaagagaa ctgataaaat ttggattttc acttgttagg gtatcaatga gtggaattat
     7801 aaaaaatatc actgttccat aaatcgaacc tgctttcaga ccaggataac gtaactgttt
     7861 cttttctttt ttcatgagtt tcctcctaat cctcatcttg atttttctta gtttttgcaa
     7921 tgcgacggga gatgaggaac tgtatgctcg ctccgaagaa aatagaaccg agaatacttg
     7981 atacaccatt tcttatagtg agaagagaat gaaaatagtc ctgaccttca tctatgagta
     8041 tactgagaag aggagttata aaaaacatcc atagaccaaa gaacaaacct gctttcagac
     8101 ctgggtagtg tagttgcttg ctttctttct cattcagcat atctggttca atgactgtga
     8161 tgcctgtttt tttcatttgg taggtgacat agccagaagc gatgagggca atcactaaaa
     8221 ttagaggagg atagattaga gccacttctt gagggtattt ataggccaga aggagtggaa
     8281 taagatttcc gaaaatcatc agataaaaga ggatgataaa gacttggttc ccaatacgat
     8341 cggcctcacg ccgtttgtat tcgtcaaggg gaccagaaat accgtatgtg cgtttgatca
     8401 gtttttcagt gaaggtttct tttttcatga gtttgctcct tttttaaaaa tcttcctccc
     8461 aaaagagact gttgaggtca gtttggaggc tgcgggcgag attgagacag agttccaagg
     8521 ttggattgta cttgtcgttt tcaatcatat tgatagtctg tctcgagaca ccgatatcct
     8581 tggcgagttc gagctgggaa atacccaatt ccttgcgaaa ttctttcaca cgattcatct
     8641 gttctccttt ttgatttatg tcgtatatat ttgactttat tatagtcttt taaacataaa
     8701 gtgtcaagta tttttgacat attttttgaa gaaatagtag tctccttgtc ctatttgtct
     8761 gacaagtgca agctggtcgg atttgtggta aaatagataa gatatgacaa aagaatttca
     8821 tcatgtaacg gtcttactcc acgaaacgat tgatatgctt gacgtaaaac ctgacggtat
     8881 ctacgttgat gcgactttgg gcggagcagg acatagcgag tatttattaa gtaaattaag
     8941 tgaaaaaggc catctctatg cctttgacca ggatcagaat gccattgaca atgcgcaaaa
     9001 acgcttggca ccttacattg agaagggaat ggtgaccttt atcaaggata acttccgtca
     9061 tttacaggca cgtttgcgcg aagctggtgt tcaggaaatt gatggaattt gttatgactt
     9121 gggagtgtct agtcctcaat tggaccagcg tgagcgtggt ttttcttata aaaaagatgc
     9181 gccactggac atgcggatga atcaggatgc tagtctgaca gcctatgaag tggttaatca
     9241 ttatgactat catgatttgg ctcgtatttt cttcaaatac ggtgaggata aattctctaa
     9301 acagattgcg cgtaagattg agcaagcgcg tgaagtgaag ccgattgaga caacgactga
     9361 gttagcagag attatcaagt tggtcaaacc tgccaaggaa ctcaagaaga aggggcatcc
     9421 tgctaagcag attttccagg ctattcgaat tgaagtcaat gatgaactgg gggcggcaga
     9481 tgagtccatc cagcaggcta tggatatgtt ggctctggat ggtagaattt cagtgattac
     9541 ctttcattcc ttagaagacc gcttgaccaa gcaattgttc aaggaagctt caacagttga
     9601 agttccaaaa ggcttgcctt tcatcccaga tgatctcaag cccaagatgg aattggtgtc
     9661 ccgtaagcca atcttgccaa gtgcggaaga gttagaagcc aataaccgct cgcactcagc
     9721 caagttgcgc gtggccagaa aaattcacaa gtaagaggga aaaagatggc agaaaaaatg
     9781 gaaaaaacag gtcaaatact acagatgcaa cttaaacggt tttcgcgtgt ggaaaaagct
     9841 ttttactttt ccattgctgt aaccactctt attgtagcca ttagtattat ttttatgcag
     9901 accaagctct tgcaagtgca gaatgatttg acaaaaatca atgcgcagat agaggaaaag
     9961 aagaccgaat tggacgatgc caagcaagag gtcaatgaac tattacgtgc agaacgtttg
    10021 aaagaaattg ccaattcaca cgatttgcaa ttaaacaatg aaaatattcg aatagcggag
    10081 taagatatga agtggacaaa aagagtaatc cgttatgcga cgaaaaatcg gaaatcgccg
    10141 gctgaaaaca gacgcagagt tggaaaaagt ctgagtttat tatctgtctt tgtttttgcc
    10201 gtttttttag tcaattttgc ggtcattatt gggacgggca ctcgctttgg aacagattta
    10261 gcgaaggaag ctaagaaggt tcatcaaacc acccgtacag ttcctgccaa acgtgggact
    10321 atttatgacc gaaatggagt cccgattgct gaagatgcga catcttataa tgtctatgcg
    10381 gtcattgatg agaactataa gtcagcaacg ggtaagattc tttacgtaga aaaaacacaa
    10441 tttaacaagg ttgcagaggt ctttcataag tatctggaca tggaagaatc ctatgtaaga
    10501 gagcaactct cgcaacctaa tctcaagcaa gtttcctttg gagcaaaggg caatgggatt
    10561 acctatgcca atatgatgtc tatcaaaaaa gagttggaaa ctgcagaggt caaggggatt
    10621 gattttacaa ccagtcccaa tcgtagttac ccaaacggac aatttgcttc tagttttatc
    10681 ggactagctc agctccatga aaatgaagat ggcagcaaga gcttgctggg aacctctgga
    10741 atggagagtt ccttgaacag tattcttgca gggacagacg gcattattac ctatgaaaag
    10801 gatcgtctgg gaaatattgt ccccggaaca gaacaaattt cccaacaaac ggtggatggt
    10861 aaggatgttt acacaaccat ttccagcccc ctccagtcct ttatggaaac ccagatggat
    10921 gcttttcaag agaaggtaaa aggtaagtac atgaccgcga ccttggtcag tgcaaagact
    10981 ggtgaaatcc ttgctaccac ccaacgaccg acctttaatg cagatactaa agaaggaatc
    11041 actaaggact ttgtttggcg tgatatcctt tatcaaagta actatgaacc agggtcagcc
    11101 atgaaggtta tgacgttagc tgcttctatt gataacaata ccttcccagg tggagaatat
    11161 ttcaatagca gtgaattaaa aatagcggat gtgacgatcc gagattggga cgttaatgaa
    11221 ggtttgaccg gcggtggtat gatgaccttt tctcaaggat ttgctcactc aagtaacgtt
    11281 gggatgaccc tccttgagca aaagatggga gatgctacct ggcttgatta tcttaatcgc
    11341 tttaaatttg gtgttccgac ccgtttcggt ttgacggatg agtatgctgg tcagcttcct
    11401 gcggataata ttgtcaacat tgcgcaaagc tcatttggac aagggatttc agtgacccag
    11461 acgcaaatga ttcgtgcctt taccgctatt gctaatgacg gtgtcatgct ggagcctaaa
    11521 tttattagtg ccatttatga tccaaatgat caaactgctc ggaaatctca aaaagaaatt
    11581 gtgggaaatc ctgtttctaa agatgcagct agtctaactc ggactaacat ggttttggta
    11641 gggacggatc cggtttatgg aaccatgtat aaccacagca caggcaagcc aactgtaact
    11701 gttcctgggc aaaatgtagc cctcaagtct ggtacggctc agattgctga cgagaaaaat
    11761 ggtggttatc tagtcgggtt aactaactat attttttcgg ctgtatcgat gagtccggct
    11821 gaaaatcctg attttatctt gtatgtgacg gtccaacaac ctgaacatta ttcaggtatt
    11881 cagttgggag aatttgccaa tcctatcttg gagcgggctt cagctatgaa agactctctc
    11941 aatcttcaaa caacagctaa atctttggag caagtaagtc aacaaagtcc ttatcctatg
    12001 cctagtgtca aggatatttc acctggtgat ttagcagaag aattgcgtcg caatcttgta
    12061 caacctattg ttgtgggaac aggaacgaag attaaaaaca gttctgctga agaagggaag
    12121 aatcttgccc cgaatcagca agtcctgatc ttatctgata aagcagagga agttccagac
    12181 atgtatggtt ggacaaaggc gactgctgag acctttgcta agtggctcaa tatagaactt
    12241 gaatttgaag gttcgggcac tactgtgcag aagcaagatg ttcgtgctaa tacagctatc
    12301 aaggacatta aaaaaattac attaacttta ggagactaat atgtttattt ccatcagtgc
    12361 tggaattgtg acatttttac taactgtagt aggaattccg gcctttatcc aattttatag
    12421 aaaggcgcaa attacaggcc agcagatgca tgaggatgtc aaacagcatc aggcaaaagc
    12481 tgggactcct acaatgggag gtttggtttt cttaattact tctgttttgg ttgctttctt
    12541 ttttgcccta tttagtagcc aattcagcaa taatgtggga atgattttgt tcatcttggt
    12601 cttgtatggc ttggtcggat ttttagatga ctttctcaag gtctttcgta aaatcaatga
    12661 ggggcttaat cctaagcaaa aattagctct tcagcttcta ggtggagtta tcttctatct
    12721 tttctatgag cgcggtggcg atatcctgtc tgtctttggt tatccagttc atttgggatt
    12781 tttctatatt ttcttcgctc ttttctggct agtcggtttt tcaaacgcag taaacttgac
    12841 agacggtgtt gacggtttag ctagtatttc cgttgtgatt agtttgtctg cctatggagt
    12901 tattgcctat gtgcaaggtc agatggatat tcttctagtg attcttgcca tgattggtgg
    12961 tttgctcggt ttcttcatct ttaaccataa gcctgccaag gtctttatgg gtgatgtggg
    13021 aagtttggcc ctaggtggga tgctggcagc tatctctatg gctctccacc aagaatggac
    13081 tctcttgatt atcggaattg tatatgtttt tgaaacaact tctgttatga tgcaagtcag
    13141 ttatttcaaa ctgacaggtg gtaaacgtat tttccgtatg acgcctgtac atcaccattt
    13201 tgagcttggg ggattgtctg gtaaaggaaa tccttggagc gagtggaagg ttgacttctt
    13261 cttttgggga gtgggacttc tatcaagtct cctgacccta gcaattttgt atttaatgta
    13321 agaatggcac cctgatgttt cagggtgttt ttgtatttaa atacacaatg aaaatcaaag
    13381 aacaaactag aaagctaact ttaggctgct caaaatatag tagattgaaa ctagaatagt
    13441 acacatctgc ttctaaaata ttgttagaaa tcgatttgac tgtcctgatc gatttgtcct
    13501 gttcttattt cattttaata tacagttttg agatggctga tagaactgac gaagtcagct
    13561 caaaacacgg ttttgaggtt gtagataagg agaagctgat gtggtttgaa gaggttttcg
    13621 aagagtgtaa aaaatattgg tcagttaaag tcaaaactct tgaccttttc tgaccaatgt
    13681 agtatattat gaacgtaagg taaatgaaag ccttactaga acacttattt aaagtaaaat
    13741 agaaagaggt gggtctatgt ttcatctaga aatcttcaga agtaaagata gtctactcct
    13801 gcttgaaaaa gaaaaaccag aaatagtacg tagggtagcg atctagctag tctaaatttt
    13861 ttaaaacaat ggtcaaagat agtcaatatc agtaatcata actaagtaaa caaaaagagg
    13921 taaagaatat gaataacaac tttaataatt ttaataacat ggatgattta tttaaccaat
    13981 tgatgggtgg tatgcgagga tacagttctg aaaatcgccg ttacttaatt aatggacgcg
    14041 aagtcacacc tgaggaattt gctcactatc gtgcgactgg tcaattacca ggaaatgcag
    14101 aaactgatgt gcaaatgcca caacaggcat caggtatgaa acaagacggt gtccttgcaa
    14161 aactaggtcg aaacttaaca gcagaagcgc gtgagggcaa gttggatcct gttatcggac
    14221 gaaacaagga aattcaagaa acatctgaaa tcctctcacg ccgcaccaag aacaatcctg
    14281 ttttggtcgg agatgcaggt gttggtaaga cagcagttgt cgaaggtcta gcgcaagcca
    14341 ttgtgaacgg agatgttcct gctgctatca agaacaagga aattatttct attgatatct
    14401 caggtcttga ggctggtact caataccgtg gtagctttga agaaaatgtc caaaacttag
    14461 tcaatgaagt gaaagaagca gggaatatta tcctcttctt tgatgaaatt caccaaattc
    14521 ttggtgctgg tagcactggt ggagacagtg gttctaaagg acttgcggat attctcaagc
    14581 cagctctctc tcgtggagaa ttgacagtga ttggggcaac aactcaagac gaataccgta
    14641 acaccatctt gaagaatgct gctcttgctc gtcgtttcaa cgaagtgaag gtcaatgctc
    14701 cttcagcaga gaatactttt aaaattcttc aaggcattcg tgacctctat caacaacacc
    14761 acaatgtcat cttgccagac gaagtcttga aagcagcggt ggattattct gttcaataca
    14821 ttcctcaacg tagcttgcca gataaggcta ttgaccttgt cgatgtaacg gctgctcact
    14881 tggcggctca acatccagta acagatgtgc atgctgttga acgagaaatc gaaacggaaa
    14941 aagacaagca agaaaaagca gttgaagcag aagattttga agcagctcta aactataaaa
    15001 cacgcattgc agaattggaa aagaaaatcg aaaaccacac agaagatatg aaagtgactg
    15061 caagtgtcaa cgatgtggct gaatctgtgg aacgaatgac aggtatccca gtatcgcaaa
    15121 tgggagcttc agatatcgaa cgtttgaaag atatggctca tcgcttgcaa gacaaggtga
    15181 ttggtcaaga taaggccgta gaagctgtag ctcgtgctat ccgtcgtaac cgtgctggtt
    15241 ttgatgaagg aaatcgccca atcggcagct tcctctttgt agggtctact ggggttggta
    15301 agacggagct tgctaagcaa ttggcactcg atatgtttgg aacccaggat gcgattatcc
    15361 gtttggatat gtctgaatac agtgaccgca cagctgtttc taagctaatt ggtacaacag
    15421 caggctatgt gggttatgat gacaatagca ataccttaac agaacgtgtt cgtcgcaatc
    15481 catactctat cattctcttg gatgaaattg aaaaggctga ccctcaagtt attacccttc
    15541 tcctccaagt tctagatgat ggtcgtttga cagatggtca aggaaataca gtaaacttca
    15601 agaacactgt cattattgcg acctcaaatg ctggatttgg ctatgaagcc aacttgacag
    15661 aagatgcgga taaaccagaa ttgatggacc gtttgaaacc cttcttccgt ccagaattcc
    15721 tcaaccgctt taatgcagtc atcgagttct cacacttgac taaggaagat ctttctaaga
    15781 ttgtagattt gatgttggct gaagttaacc aaaccttggc taagaaagac attgacttgg
    15841 tagttagtca agcggctaaa gattatatca tagaagaagg ttacgacgaa gtcatggggg
    15901 ttcgtcctct ccgtcgcgtg gttgaacaag aaattcgtga taaggtgaca gacttccact
    15961 tggatcattt agatgctaaa catctggaag cagatatgga agatggcgtt ttggttattc
    16021 gtgagaaagt ctaagacaga attttgagga taaaaaagaa ggagccagct gaaaaaaact
    16081 ggttcctttt gaggtatgac gggcatgtcg tatagtagaa gtgtattatt ctagtttcaa
    16141 tttactatag tagctcagaa gtcggtactt aaacgtgcta tatcaaaacc agtccttgaa
    16201 aaacgtggac tggtttcgtg tttggattat taccttgaac gacatgcgtt aaaagttagt
    16261 tgaacagccg tatgccgaac ggcacgtacg gtggtgtgag aggggctaga gattatcccc
    16321 tactcgattt taaatcacat gacgttcaaa ggcatcatct gaaatccctt gttccaagat
    16381 gagttttgcc cattctttag cagagaagag gctgtggtcc ttgtagtttc cgcaagattc
    16441 gatggttgtc cctgggacat cttcccaagt agtagtttca gcgatttcct tgagcgaatc
    16501 cttgataaca gctgcgattt tagcactggt gtgacgtccc cacataatca tgtggaagcc
    16561 tgtgcggcaa ccaaatggtg aacagtcaat catgccgtca atgcgggtac ggatgagttt
    16621 ggctaagagg tgctcgatag tgtgaaggcc ggcagtaggg atagagtctt cgtttggttg
    16681 caccaagcga atatcataat tggagatgat gtctcctttt ggtcctgttt cttccccaat
    16741 caagcgaaca tagggtgctt tgacaatggt gtggtcaagt tcaaaacttt cgacaataac
    16801 ttcttttgac atggtaaatc ctttcagttt tcttctctca ttatatcata aaggttgctc
    16861 ctgagacaga gagaaaacct ctccgaggct ggagaggttg aaatctttac ttacgatata
    16921 agcggtcgta ttggtagtat gggtcaaagg ttacgttgat acccagttta cgaaggacat
    16981 tcttgtcttc atcagtcaag atgatggttg agtgggcttc gcttcctttg aggttgccga
    17041 gttcttccat agcgcgggca gcatcaggat tttctgtagc tgtgatagca agtgcaatca
    17101 ggatttcatt tgaatgaagg cgtggattgc ggctaccgag atgatcgatt ttaagacctt
    17161 ggattggctt aacaacttca ggctcgatta gttttacttc tttagcgatg tcagctgatt
    17221 ttttgatggc attgatcaag gcagcggctg tagggccaaa gagttctgag ttcttaccag
    17281 tgatgatttc cccatttggc aattcaaagg ctagggctgg tccaccagtt tcttctgctt
    17341 tttggcgcgc aacgacagca accttacggt ctgcaggtgt gataccgagg tcgttcatga
    17401 gcaactcaat tttcttgacg gcagcttcgc caactttttc agctttgaag tcaagaactg
    17461 tttgatagta acggcggatg atttcttgtt tagaagcttc gacagcagcc tcgtcatctg
    17521 taatagcgaa accaaccatg ttgacaccca tatctgtcgg tgaagcgtat ggtgattttc
    17581 cgagaatacg ttccaacatg cgcttgagca ctgggaagat ttcgatatca cggttgtagt
    17641 tgacagtggt ttctccatag gtttgaagat ggaaggggtc aatcatgttg acatcatcaa
    17701 ggtcagctgt ggcagcttca taagccaagt taactggatg atgaagggga agattccaaa
    17761 cagggaaggt ttcaaattta gcgtagccag atttgatgcc attgatttgg tcgtggtaca
    17821 tattggacat acacgttgcc aattttccag aaccgggtcc aggagcggtt acgacaatca
    17881 agttgcgact ggttttgatg tagtcgtttt tgcccatgcc ttctggggaa atgatgtgat
    17941 ccatatccgt cggatatcct ttgattggat aatgaagata agaatcaatt ccgtttttct
    18001 caagttggtt gcggaaggca tctgcagcgg gttggccagc gtattgtgta atgacaacgg
    18061 aaccaacaaa aatccctaat tcattgaatt tgtcaatcaa acgaagaact tcttggtcat
    18121 aagaaatgcc caagtcgcca cgtgctttgg aatgttcaat gttgctagca ttaatggcaa
    18181 tcacaacctc aacctgctct ttcaattctt gcaagagctt gattttgttg tcaggttcat
    18241 aaccaggaag gacacgagca gcgtggaaat cttctaacat tttgccacca aactctaggt
    18301 aaagcttgcc gtcaaattgg ttaatgcgct ctaagatgtg gtcgcgttgt aaattcaaat
    18361 actgttcaga actaaaagct tgttttttca tttttttacc tctggactct attataataa
    18421 aaaattggaa gttaggaaac tacggagcta aaaaataaat taaaaagatt aagcaaacgc
    18481 ttgcacaaaa ttttaaaaag tgctatcata tactatagat tattaaaata atgaggtaaa
    18541 cagatgcaag aaaaatggtg gcacaatgcc gtagtctatc aagtctatcc aaagagtttt
    18601 atggatagta atggagatgg agttggtgat ttgccaggta ttaccagtaa gttggactat
    18661 ctagccaagc taggaatcac agcaatttgg ctttctcccg tttatgacag ccctatggat
    18721 gataatggct atgatattgc tgattatcaa gcgattgcgg ctatttttgg aaccatggag
    18781 gacatggatc agctgattgc agaagctaag aagcgtgaca ttcgtatcat catggacttg
    18841 gtggttaatc atacctcaga tgaacatgct tggtttgtcg aagcctgtga aaatactgac
    18901 agccctgagc gagactacta tatctggcgc gatgaaccca atgacctaaa ttctatcttt
    18961 agtgggtctg cttgggaata cgatgaaaag tcaggtcaat actatctcca ctttttcagc
    19021 aagaaacagc cggatctcaa ctgggaaaat gaaaaacttc gccagaaaat ttatgagatg
    19081 atgaacttct ggattgataa aggtattggt ggtttccgta tggatgttat tgacatgatt
    19141 ggcaaaattc ctgacgagaa ggtagtcaat aatggtccta tgctccatcc ctatctcaag
    19201 gaaatgaatc aggcgacctt tggagataag gatctcttga cagtagggga gacttgggga
    19261 gcaacgccag cgattgccaa gctttactct gatccaaagg ggcaagaatt gtctatggtc
    19321 ttccagtttg aacatatcgg tcttcagtat caggaaggtc agcctaaatg gcactatcaa
    19381 aaagagctga atatcgctaa gttaaaagaa atcttcaaca aatggcagac agagttagga
    19441 gttgaggacg gttggaattc cctcttctgg aacaatcatg acctccctcg tatcgtctca
    19501 atctggggaa atgaccaaga ataccgcgaa aaatctgcca aagcctttgc aatcttgctt
    19561 catcttatga gaggaactcc ttatatctac caaggtgagg agattgggat gaccaactat
    19621 ccgtttgaaa cactggatca agtagaagat attgaatctc tcaactatgc gcgtgaggct
    19681 cttgaaaaag gtgttccgat tgaagaaatc atggacagta tccgtgttat tggacgtgac
    19741 aatgcccgta cccctatgca atgggacgag agcaaaaacg ctggtttctc aacagatcaa
    19801 ccttggttgg cagttaatcc aaattacgag atgatcaacg tccaagaagc gctggcaaat
    19861 ccagattcta ttttctatac ctatcagaaa ctggtccaaa ttcgcaagga gaatagttgg
    19921 ctaattcgag ctgactttga attgcttgat acggctgata aggtctttgc ttatatacgt
    19981 aaggatggcg accgtcgctt cctagttgtg gctaacttgt ccaatgaaga gcaagacttg
    20041 acagtagaag gaaaagtcaa atctgtcttg attgaaaaca ccctagctca agaagtcttt
    20101 gaaaaacaaa tcttagttcc atgggatgct ttctgtgtgg aattactata aatatttttt
    20161 gcagaaaaat ttaaaattga aatcatataa aaacaaggga ggactgtata aaaggcggaa
    20221 atcctttgtt ttttataacc aaggtttata aactttcatt ctcgaaattc aattaacttt
    20281 acaaattccc actattaagg agaaagaaga cgaacataaa gaagcgtatc cttagtgcag
    20341 gcctgacttt tgcatctgct ttgcttttag ctgcttgcga ccaatcaggt tcagatacaa
    20401 aaacttactc atcaaccttt agtggaaatc caactacatt taactatcta ttagactatt
    20461 acgctgataa tatagtcaat tgaaacaaga gaaagacaaa agagcctcgt aaaaggtatt
    20521 gcaacttggt aatacctttt tgaggtgctt tttgatatga gcccatgttt tctcaatagg
    20581 attgtactca ggtgagtagg gaggaagagg taaaagttta tgcccaaact cttcgcataa
    20641 aagttctagc ttccccattc tatggaatct tgcattatcc ataataataa ccgatggtgt
    20701 ggttaatgtt ggtaagaaaa acttctgaaa ccaagcttca aaaaagtcgc tcgtcatcgt
    20761 ctcttcgtaa gtcattggaa cgattaactc accatttgtt agacctgcaa ccaaagaaat
    20821 cctctgatat cttcttccag atactttgcc tcttcttaac tgacctttta atgagcgacc
    20881 atattctcga taaaaataag tatcgaatcc tgtttcatca atctaaacag gtgctaggtg
    20941 ctttaaacta ttaaaattct taagaaataa ggctactttt tctgggtctt gttcataatt
    21001 agattgaaac tagaatagta cacctctgct tctaaaacat tgttagaaat cgatttgact
    21061 gtcctgatcg atttgtcatg ttcttatttc attttactat atttttgttt cgcgggaagt
    21121 ctactaagat acttaaagat gcagatagta aaaaaaaatg tagacattac cgtaaaaaag
    21181 tgatataatc gtatgatgtt caaggtatag gtgttaatca tgagtagacg ttttaaaaaa
    21241 tcacgttcac agaaagtgaa gcgaagtgtt aatatcgttt tgctgactat ttatttattg
    21301 ttagtttatt ttttattgtt cttaatcttt aagtacaata tccttgcttt tagatatctt
    21361 aacctagtgg taactgcgtt agtcctacta gttgccttgg tagggctact cttgattatc
    21421 tataaaaaag ctgaaaagtt tactattttt ctgttggtgt tctctatcct tgtcagctct
    21481 gtgtcgctct ttgcagtaca gcagtttgtg ggactgacca atcgtttaaa tgcgacttct
    21541 aattactcag aatattcgat cagtgttgct gttttagcag atagtgagat tgagaatgtt
    21601 acgcaactga tgagtgtgac agcaccgact gggactgata atgaaaacat tcaaaaacta
    21661 ctagctgata tcaaatcaag tcagaatacc gatttgacgg tcaatcagag ttcgtcttac
    21721 ttggcagctt ataagagttt gattgcagga gagactaagg ccattgtctt aaatagtgtc
    21781 tttgaaaata tcatcgaatc ggagcatcca gattacgcat cgaagataaa aaagatttat
    21841 accaagggat tcactaaaaa agtagaagct cctaagacgt ctaaggatca gtctttcaat
    21901 atctatgtta gtggaattga tacttatggt ccgattagtt cggtgtcgcg ttcagatgtc
    21961 aatatcctga tgactgtcaa tcgagatacc aagaaaattc tcttgaccac aacgccacgt
    22021 gatgcttatg taccaatcgc agatggtggg aataatcaaa aagataaatt gacccatgcg
    22081 ggcatctatg gagttgattc gtccatccac actttagaaa atctctatgg agtggatatc
    22141 aattactatg tgcgattgaa cttcacttct ttcttgaaat tgatcgattt gttgggtggg
    22201 gtagatgttt ataatgacca agattttaca tctctacacg ggaagttcca tttcccagtt
    22261 ggaaatgtcc acttagattc tgagcaggct ctgggatttg ttcgtgagcg ctattcccta
    22321 gcggatggtg atcatgaccg cgggcgcaat caacaaaagg tgattgcggc catccttcaa
    22381 aaattaactt cgagcgaagc gctgaaaaat tatagtagga tcattgatag cttgcaagat
    22441 tctatccaaa caaacatgcc acttgagacc atgataaact tggtcaatgc tcagttagaa
    22501 agtggtggaa cgtacaaagt gaattcgcaa gacttgaagg gtatgggacg gacggatctt
    22561 ccttcctatg cgatgccaga tagtaacctc tatatgatgg aaattaacga cagtagcctt
    22621 gcatctgtca aaactgctat tcaggatatg ttggagggca gatgaaatga ttgatattca
    22681 ttcgcacatc gtctttgatg tagatgatgg tcccaagtca agagaggaaa gcaaggctct
    22741 cttggcagaa tcctacaggc agggggtgcg aaccattgtc tctacctctc accgtcgcaa
    22801 gggcatgttt gaaactccgg aagagaagat agcagaaaac tttcttcagg ttcgggaaat
    22861 agctaaggaa gtggcgagtg acttggtcat tgcttacggg gctgaaattt actacacatc
    22921 agatgttttg gataagctgg aaaaaaatcg gattccgacc ctcaataata gtcgttatgc
    22981 cttgatagag tttagtatga acacccctta tcgcgatatt catagtgcct tgagtaaaat
    23041 attgatgttg ggaattactc cagtcattgc ccacattgag cgctatgatg cacttgaaaa
    23101 taatgaaaaa cgcgttcgag agctgatcaa tatgggctgt tacacgcaag taaatagttc
    23161 acatgtcctc aaatccaaac tttttggaga acgttataaa ttcatgaaaa aaagagctca
    23221 gtattttttg gagcgtgact tggttcatat cattgcaagt gatatgcata atgtggacag
    23281 cagacctccc catatggcag aagcatatga ccttgtttcc caaaaatacg gagaagcgaa
    23341 ggctcaggaa ctttttatag ataatcctcg aaaaattgta atggatcaac taatttagga
    23401 gaaatgatga aagaacaaaa cacgatagaa atcgatgtat ttcaattact taaaaccttg
    23461 tggaaacaca agctaataat tttattagtg gcacttgtga caggggcggg agcttttgca
    23521 tatagcactt ttattgttaa gccagaatat acaagcacca cgcgtattta cgtagtcaac
    23581 cgtaatcaag gagataagcc gggactgacg aatcaggact tgcaggcagg aacttatctg
    23641 gtaaaagact accgcgaaat tatcctttcg caagatgtat tggaaaaggt agcgacaaat
    23701 ttgaaattgg atattccagc aaaaacgtta actagcaaag tgcaagtgac tgtaccagcc
    23761 gacactcgta tcgtctcaat ctctgtcaag gataaacaac cagaggaagc cagtcgcatt
    23821 gctaattctc tacgagaagt tgctgcagaa aagatcatcg ctgtaacgcg agtatctgat
    23881 gtaacgacgc ttgaagaagc gcgaccagct atgactccct cttctccaaa tgttcgacgc
    23941 aacaccttag ttggttttct tggaggagcc gccgtaacag taattactgt tcttttgatt
    24001 gagttgctcg atacccgtgt aaaacgtcct gaagatgttg aagatgtact gcaaatgcca
    24061 cttctaggag tcgttccaga ttttgataaa atgaaatagg aggaagttat gccaacgtta
    24121 gaaatctcac aggcgaaatt agaacttgtg aaaaaggcag aggaatatta taatgctttg
    24181 tgcacgaacc tacagttaag tggagatgat ttgaaagtat tttctatcac ttctgtgaaa
    24241 gcaggagaag gaaaaacaac gacttccacc aatatcgctt gggcttttgc gcgtgcaggt
    24301 tacaaaacgc ttctgattga tgcagacatg cgtaactccg ttatgtcagg tgtctttaaa
    24361 tcaagggaaa ggattactgg actaacagaa tttctatcag ggacaacaga cctgtcacag
    24421 gggctttgcg ataccaatat tgagaatctc tttgtgattc aggctggctc tgtgtcacca
    24481 aatccgacag cccttcttca aagtaagaat ttcagtacaa tgcttgaaac cttgcgtaaa
    24541 tattttgact atatcgtcgt agacactccc cctgtcggtg tcgtgattga tgcggctatc
    24601 attatgcaga aatgtgatgc ttctatttta gtgacgaagg caggtgaaac aaaacgacgg
    24661 gatcttcaaa aagcgaaaga acagttggaa caaactggga agtcgtgttt aggagttgtg
    24721 ttgaataaac tcgatacttc agtagagaaa tacggttctt atgggagtta tgggaattac
    24781 aggaaacaga aaaaataggt tgagggatag aaatgagtga aaaactagca aagccttcag
    24841 tagccatagt ccagagtttt cttgttattt tattgactta tctacttagc gctgtgagag
    24901 aaacagagat tgtttcaaca acagctattg tactttatat cctccactat tttgtctttt
    24961 atatcagtga ttatggacgg aatttcttta aaaggggata tttgattgaa cttgtccaga
    25021 cattgaaata tatcctattc tttgcgctag cgattagtat ttctaatttt ttcttagaag
    25081 atcgatttag tatttccaga agaggcatga tttacttcct cttattacat gttttcttag
    25141 tctatgtgct aaacccattt atcaagtggt attggaagcg ggcttatccc aactttaaag
    25201 gaagtaagaa gattctccta cttacagcaa cttctcgtgt tgaaaaggta ctggatagat
    25261 taatagaatc aaatgatgtt gttggggagt tggtagccgt cagtgtttta gataaaccag
    25321 attttcagca tgatgattta aaggttgtag cagaggggga gatagtaaat tttgcgactc
    25381 atgaggtggt cgatgaagtc tttatcaatc ttccaagtga aaaatacaat attggagagc
    25441 ttgtctctca gtttgaaacg atgggaattg atgtaacagt caatctaaat gctttcgatt
    25501 gggcacgtaa caagcaaatt tgtgagatgg caggactaaa tgttgtgact ttttctacaa
    25561 cattttataa gactagccat gtgattgcta agcggattat tgatattgtc ggttccctgg
    25621 taggtttgat attatgtggt ctagtcagta ttgtgctggt tcctttgatt cgaaaggatg
    25681 ggggttctgc tttttttgct cagacgcgta taggaaaaaa tggccgccat tttacttttt
    25741 ataagtttcg ctctatgtgt gtggatgctg aggagaaaaa aagagaactc atggaacaaa
    25801 ataccatgca gggtggaatg tttaaggtgg acgatgatcc tcgtatcacg aaaattggtc
    25861 attttatacg gaagacgagc ttggacgagc taccacagtt ttataatgtt ctaaagggag
    25921 atatgagttt ggtaggtaca cgaccaccaa cagtggacga gtatgagcac tataccccag
    25981 aacaaaaacg tcgcctaagt tttaaacctg gcataacagg tttatggcag gtcagcggac
    26041 gaagtgaaat caaaaatttc gatgaagttg tcaaattaga tgtggcctat attgatggtt
    26101 ggacaatctg gaaagatatt gaaattttat tgaagacagt taaggttgta ttgatgaagg
    26161 atggagcgaa atagttcata tgaatgagag aattcaaata ttaggaatta ctatagatcc
    26221 attaacgatg aaagaaacag tggatgctgt tgaacagtat gttttaaaga agcacccttt
    26281 gcacttgatg ggagttaacg cggataaaat taatcagtgc tatgaagatg agaaaatcaa
    26341 aaaaatcgtt aatgaatcag gaataattaa tgccgacggt gcttcagtag ttttagcgag
    26401 taagttttta ggaacaccag ttcctgagcg agttgctgga atagacttga tgaaacattt
    26461 actagagttg tctaatgaaa aaggatactc ggtctacttt tttggagcaa aagaagacgt
    26521 tttacaagat atgttaaaaa tttttgaaga aagatatcca gctctaaatg ttgttggcta
    26581 tagaaatgga tatttttcgc ctgaagatga aaaacatatt caggaagata ttaaagagaa
    26641 aaaaccagat tttgtgtttg taggaattac ttctcctaag aaagaatata ttatccaatc
    26701 attcatggat aatggaatta atgctgtgtt tatgggggtt ggtggaagtt ttgatgtact
    26761 atcagggcat atcaaacgag ctccattatg gatgcaaaat gctcacctag agtggttgtt
    26821 tcgtgtggca aatgagccta aacgtctctt taagcgttat tttgtaggga atgtttcatt
    26881 tataagaaaa gttttaagag cgaaaagggg atataaactt tgaaccaaac agaaatagtt
    26941 aaattgattc agaaagttga attggatgct ataaaagagt ttcaaaaaat ttgtaaagag
    27001 aacaatatag atttcttcct tcgtgggggg agtgtacttg gggcagtcaa atatgatggc
    27061 tttattccat gggatgatga catggatatt gctgtgcctc gtgaaggcta cgataaactt
    27121 ccaggtatct ttaaggatag aattatcgca ggcaaatatc aggttcttgc ttatcaatat
    27181 tgcgatactt tacattgcta ctttcctcgc ttattccttt tagaagatga aagaaaacgt
    27241 ttgggattgc cacgaaatac caatttaggt ttgcatttga ttgatatcat tcctttggat
    27301 ggtgccccaa atcattcatt tttaagaaag ctttacttcg gtaaagtata ctggtatcgt
    27361 tttttagcaa gtttaggaac tacctatgtt ggagaccatg tggatatgca ttccgctaag
    27421 caaaaactaa tcattggttt ctttaaaaag ctaggatttg caaaactatt ccctcaaaat
    27481 tatgtgtaca gacgcttgga taatctctat aaaaagtatg attggaaaaa gcagaagtat
    27541 gctgggacaa ttaatgcatc tctgtttgcc aaagaagtca tgccagtaga gatttgggga
    27601 gaaggagtag agaagccttt tgaggatgcc ttctttaaag ttcccacgga gtatgaccgc
    27661 tatctgaaaa gactttacgg agaaaactat cttcacgaag agcctagtga tgatgaaaag
    27721 aaatcgcatt taggaggaca ataatttgtt ttgttatatt attttgcatt acaaagtctt
    27781 agaggaaact atttcttgtg ttaaatcaat aaaagaaggc aattccaata caaagcagat
    27841 cgttattatt gataattttt ctaataacgg tacgggtgaa aaactacaag agctgtatga
    27901 gtcagattca gagattgatg tcttgattaa ccatgaaaat gctggtttcg ctcgaggtaa
    27961 taatgtagca tatcagtttg ctaaggaaaa gtacaaacct gattttatgg ttatcatgaa
    28021 taatgatatt gagatagaaa cagaagagtt tgaaaaaatc gtgacagata tctatcggaa
    28081 ggaaaaattc catttgttag gaccagatat cttttcgacg acgtatcagc ttcaccaaaa
    28141 cccaaaacgg ttgacgcatt atacttatga agaggttaag gctctcaatg aaaaatttaa
    28201 gaaagggagc caagttagtc tagcattaaa aattaaatgt tggttgaagt ctagtaaagt
    28261 tcttcggaca gcaatctatc aaaataggcg taaaaagaaa tcagtagact atagaaaaca
    28321 ggtagaaaac ccaattcttc atggttcgtt tattgtatat tctagagatt ttattgagaa
    28381 agaggagtat gcttttaatc ccaatacctt cttctattat gaaacagaga tattagatta
    28441 tgaagctgag ttaaaaggat ataagagaat ttatacaccg aagattaagg tcttgcacca
    28501 tcaaaatgtt gcaaccaatc aagtctatac aaacttagta gaaaaaacct tgttttcaaa
    28561 caaatgcaac tttgaatcca ctagttattt tttgaagttg atgaaagaaa acgagggtgt
    28621 gtaaatgact tatttatttt tactctgcct gaccttattc ttattaactt tcttctattt
    28681 ttttgctttc aatcaagatt tgatagctcc gccagttgtc atgtctgtta tgtttctcat
    28741 tagttctgta tttgccctta ttaatgtgca aaactggaat attgagtata gtggtttagc
    28801 ttatcttttg attattagtg gcattattgt tttttcaatg cctctcttgg ctcttaattc
    28861 gcctagtcta aataccaaga ttaaagtgac ggatcggctc attgatatcc aattctggaa
    28921 aatagctctt actattatag ttgacctcgt tattctatat ctttatagga gagagattca
    28981 taatcttgca cttagccatg gttatacggg ttcaaatttt cagtggttct ttagaaatgc
    29041 taccagttat gaaggtgagc taacagtgcg aacttcgatt cgggtcctca ttcgtatcat
    29101 tgacgtatct gcttatattt ttggatatac ttttattaat aatttcttca tttatagtca
    29161 taaacgctct aaagatttac tgctcttagt tccattcttg atttttattt ctaaaacctt
    29221 attatctggg ggtagattgg atattataaa aattttaatt gcgtatgttg taatggccta
    29281 tattcagcaa aaacgaaaag ttggctggga taaggtcatc tcccataaat atatgagact
    29341 tggttttgta ggcttgatag ctgggattcc taccttttac tattctttat tcttatctgg
    29401 acgttctacg actagaactg tatttgaaag tatttcaacc tatttaggag gttcgattca
    29461 gcattttaat cagtatattc aaaatcctat tggagtagct gaggtttttg gcgatgagtc
    29521 atttgtagct attatgaata ttttgggtaa tcttggcttt gtcaattaca atagtaccgt
    29581 tcacttagaa tttcggcagt tagggattac tatgggtaac gtttatactt tctttagaag
    29641 accctggcat gactttgggt tagtcggtat gtatatcttt tcctttgttg tcggtgtgtt
    29701 ttttgctatt ttttatttga aattaagaaa gagccgtgct ggttttaaat tggatattca
    29761 tacaattatt tactcttatt tcttttattg gattttttta tcgtccattg agcaatactc
    29821 gtttacaaca atcagtttgt ttacacttgt atttatagtc ttggtttacc taatggcttt
    29881 cttctattgg aatttggatt tccacagagg aaaactagta attaaactct ctgacacaag
    29941 tattaaatcg gaaaactgaa aacaataaaa atagagagga tagttgatga atagcaaaat
    30001 taaaaatata ctaactaatt tctcttatgt catttcttca aatcttttga cagtcttgac
    30061 ctcttccttg gttgtcttaa ttttccccaa attaatgggg gtaacagagt acagttactg
    30121 gcaactttat attttttatc t
//
LOCUS       DAVHEW010000013        28000 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVHEW010000013 DAVHEW010000000
VERSION     DAVHEW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.013"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            353..1516
                     /locus_tag="VVL53_000583"
     CDS             353..1516
                     /locus_tag="VVL53_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU4182793.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1513..2190
                     /locus_tag="VVL53_000584"
     CDS             1513..2190
                     /locus_tag="VVL53_000584"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU4182794.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2180..3034
                     /locus_tag="VVL53_000585"
     CDS             2180..3034
                     /locus_tag="VVL53_000585"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU4182795.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMILLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVVDIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3053..4120
                     /locus_tag="VVL53_000586"
     CDS             3053..4120
                     /locus_tag="VVL53_000586"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU4182796.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKDLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4130..5296
                     /gene="aroC"
                     /locus_tag="VVL53_000587"
     CDS             4130..5296
                     /gene="aroC"
                     /locus_tag="VVL53_000587"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU4182797.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5306..6409
                     /locus_tag="VVL53_000588"
     CDS             5306..6409
                     /locus_tag="VVL53_000588"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU4182798.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6420..6758
                     /locus_tag="VVL53_000589"
     CDS             6420..6758
                     /locus_tag="VVL53_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU4182799.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6850..8133
                     /gene="aroA"
                     /locus_tag="VVL53_000590"
     CDS             6850..8133
                     /gene="aroA"
                     /locus_tag="VVL53_000590"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU4182800.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLINPNSHLVLQNVGINETRTGIIDVIRAMGGKL
                     EVTEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8126..8602
                     /locus_tag="VVL53_000591"
     CDS             8126..8602
                     /locus_tag="VVL53_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU4182801.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIENRLGMSIANFFA
                     EKGETAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLRADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8599..9447
                     /gene="pheA"
                     /locus_tag="VVL53_000592"
     CDS             8599..9447
                     /gene="pheA"
                     /locus_tag="VVL53_000592"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU4182802.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESCPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9444..10718
                     /gene="psr"
                     /locus_tag="VVL53_000593"
     CDS             9444..10718
                     /gene="psr"
                     /locus_tag="VVL53_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU4182803.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10997..11842
                     /locus_tag="VVL53_000594"
     CDS             10997..11842
                     /locus_tag="VVL53_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU4182804.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGDL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11832..12935
                     /locus_tag="VVL53_000595"
     CDS             11832..12935
                     /locus_tag="VVL53_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4182805.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12937..13923
                     /locus_tag="VVL53_000596"
     CDS             12937..13923
                     /locus_tag="VVL53_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4182806.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13939..15372
                     /locus_tag="VVL53_000597"
     CDS             13939..15372
                     /locus_tag="VVL53_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU4182807.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15401..16435
                     /locus_tag="VVL53_000598"
     CDS             15401..16435
                     /locus_tag="VVL53_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU4182808.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANNLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16635..17372
                     /gene="mecA"
                     /locus_tag="VVL53_000599"
     CDS             16635..17372
                     /gene="mecA"
                     /locus_tag="VVL53_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU4182809.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEVKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17523..18809
                     /locus_tag="VVL53_000600"
     CDS             17523..18809
                     /locus_tag="VVL53_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU4182810.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18811..19680
                     /locus_tag="VVL53_000601"
     CDS             18811..19680
                     /locus_tag="VVL53_000601"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU4182811.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19756..19827
                     /locus_tag="VVL53_000602"
                     /pseudo
     CDS             19756..19827
                     /locus_tag="VVL53_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            19975..20913
                     /gene="msrB"
                     /locus_tag="VVL53_000603"
     CDS             19975..20913
                     /gene="msrB"
                     /locus_tag="VVL53_000603"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4182812.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20971..22695
                     /locus_tag="VVL53_000604"
     CDS             20971..22695
                     /locus_tag="VVL53_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182813.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22697..24445
                     /locus_tag="VVL53_000605"
     CDS             22697..24445
                     /locus_tag="VVL53_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182814.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24747..26006
                     /locus_tag="VVL53_000606"
     CDS             24747..26006
                     /locus_tag="VVL53_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU4182815.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26082..26217
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26256..26756
                     /locus_tag="VVL53_000607"
     CDS             26256..26756
                     /locus_tag="VVL53_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU4182816.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26832..27200
                     /gene="rplL"
                     /locus_tag="VVL53_000608"
     CDS             26832..27200
                     /gene="rplL"
                     /locus_tag="VVL53_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU4182817.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27387..27974
                     /locus_tag="VVL53_000609"
     CDS             27387..27974
                     /locus_tag="VVL53_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4182818.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQVLYGASLFS
                     FLWMYGQRKQVVKVNMKTRMKCLGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNFLVYSLLLF"
BASE COUNT         8266 a         5203 c         6297 g         8234 t
ORIGIN      
        1 aaaaggcttc tctaaaaatc aaattttcca gatacctact gaagctgatt tatttcttca
       61 tctttcttct actacctttg tcactaaatt tgacagacac atattgctga tacaacctct
      121 ctacctataa tctcacagtt aacctgaggc tagtttcata gtttgctatt tgattttcgt
      181 tgataataaa aaatgctcct ccaagacgga agagcatttt ttattgtgat tcttacacat
      241 ttacttccta gttttgcagg aagtctagta taccattttt taaaagaaga gtaaaaaagc
      301 aagggtagtt tcttttttga gaattgtcta aaagtttgat ataatggtag ttatgaatag
      361 aataagagtc agcaaaaggg ttgaaaagaa gcttgctaag gggctagttt tactagaagc
      421 cagtgatctt gagaatgtca atcttaagga tcaggaagta gaggtgcagg gtcaggaagg
      481 aaactttctt gggactgcct acctttctca gcaaaacaag ggcttgggct ggtttatcag
      541 caaagacaag gtggccttca atcaagcttt ctttgaaacg ttgtttagaa aagccaaaga
      601 aaagagaagc gcctactatc aagatgattt aacaactacc tatcgtctct ttaatcaaga
      661 gggagatggc tttgggggtc tgacagtgga cctttatggc gactacgccg tcttttcttg
      721 gtataactct tatgtttatc agattcgtca gactatatca gaagccttta gacaggtttt
      781 ccctgaggtt ttaggggctt atgagaaaat ccgctttaag ggtttggact atgaatctgc
      841 ccatgtttat ggtcaagaag cacctgactt tttcactgtt ttagaaaatg gtgtcctgta
      901 tcaagtcttt atgaatgatg gcttgatgac aggaattttc ctagaccagc atgaggttcg
      961 cggtagttta gttgacggct tggctatggg taaatccttg ctcaatatgt tttcctacac
     1021 agcggctttt tcagtagctg cggccatggg aggagctagc cagacaactt ctgttgatct
     1081 agccaaacgt tcacgagaat tgtctcaagc gcattttcag gcaaatgggc tcagcacaga
     1141 cgagcatcgt tttatagtca tggatgtctt tgagtatttc aaatatgcca aacgcaaaaa
     1201 cttgacctac gatgtgattg tcctagatcc gcctagcttt gctcggaata aaaaacaaac
     1261 tttctctgtg gccaaggatt atcacaagtt gatttcccag agtcttgaga ttttaaatcc
     1321 gggagggatt atcattgcca gtaccaatgc tgccaatgtt tcccgtcaga aatttacaga
     1381 acaaattgat aaaggctttg caggaagaag ttaccagatt ttaaacaaat atggtcttcc
     1441 agcagatttt gcctataata aaaaagatga aagtagtaat tacctcaagg tgattagtat
     1501 gaaggttagt aaatgaaatt aatcgtttca gtaatgccaa gaagtttaga ggaggctcag
     1561 gctctggatg ccacgaggta cctggatgcc gacatcattg aatggcgtgc cgactatctg
     1621 cctaaagaag cgattttgca ggtagctcca gccatttttg aaaaattcgc aggccgtgag
     1681 ttggttttca cgctacgaac tcgctccgaa ggtggagaaa tcgacctttc tccagaagag
     1741 tatatccatc taatcaagga agttgcgcaa ctctatcaac cagactatat tgattttgag
     1801 tactatagct acaaggatgt ttttgaggaa atgctggact tcccaaatct tgttttgagt
     1861 taccataatt tccaagaaac acctgagaac atgatggaaa tcttgtcaga gttgacgatt
     1921 ctaaatccaa aacttgttaa ggttgcggtg atggctcaca cggagcagga tgtcttagac
     1981 ttgatgaact atacacgagg ctttaaaacc ctcaatcctg aacaggaata tgtgaccatt
     2041 tctatgggta aggtgggcaa ggtctctcgt atcactgcgg atgtgactgg ttcgagttgg
     2101 tcttttgcca gtctggatga ggttagtgcc cctgggcaga tttctctagc tagcatgaaa
     2161 aaaatcaggg agattttgga tgaagcttga tggctataca cgtttagctg cagttgttgc
     2221 caatcctatt aagcattcta tttctccctt catccacaat agagcctttg aggcgacagc
     2281 taccaacggt gcttatgtgg cttgggagat tgaagcgagt gacttggtag aaacagtggc
     2341 caatattcgt cgctaccaga tgtttggcat caatctgtcc atgccctata aggagcaggt
     2401 gattccttat ttggataagc tgagcgatga agcgcgcttg attggtgcgg ttaatacggt
     2461 tgtcaatgag aatggcaatt taattggata taatacagat ggcaagggat tttttaagtg
     2521 cttgccttct tttacaattt caggtaaaaa gatgatcctg ctgggtgcag gtggtgcggc
     2581 taaatcaatc ttggcacagg ctattttgga tggcgtcagt cagatttcgg tctttgttcg
     2641 ttccgtttct atggaaaaaa caagacctta cctagacaag ttacaggagc agacaggctt
     2701 taaagtggat ttgtgtgctt tagaatatgt ttctgaactg caagcaagga ttgccgagtc
     2761 ggatttgtta gttaatgcca ccagtgtggg catggatggc caatcctctc ctgttcctga
     2821 aaacatagtc ttaccagaaa ctctcttagt tgtagatatc atataccaac cctttgaaac
     2881 accgtttttg aaatgggcta gaagtcaggg caatccagca gtcaatggtc tgggaatgtt
     2941 gctctatcaa gctgcagaag cttttcaact gtggacaggc aaggaaatgc cgacagaaga
     3001 gatttggcag tctttaacag aaaaatacca ataaagaaag ggagactctc ctatgaaaat
     3061 cagaatcgat attcctcatc atccttatga tattcagatt gaaaaaggtt gtatggccca
     3121 ggctggtcag tggttgcgag aactctggca accgcaaaag gtagtcattg tgacagataa
     3181 ccatgtagcc tctctctatg cagagaaggt caagctcagc ctagaagatg ctggttttca
     3241 ggtagctgtt tttgatttct tagaaggtga agaaagaaag gatttaacta ctgttcagaa
     3301 agtctatgaa tttctagtca agcaaggtct gactcgtagc gatggaatcg ttgctcttgg
     3361 tggtggcgtt gttggggacc tggctggatt tgtagcctct acctatatgc ggggtattca
     3421 ctttgttcag attccgacta gtttgacagc tcaggttgat tcttctatcg gtggaaagac
     3481 aggcgttaac acaccatttg ctaaaaatat ggtggggacc tttgcccaac cagatggggt
     3541 tctgattgat ccacttgttc ttgaaaccct cggaaaaaga gagttgattg aagggatggg
     3601 tgaagttatc aagtatggct tgattgagga tccagaactg tgggctctct tgacggggct
     3661 gaatggttct gttgagagca ttttggaaca tgcagagacc ttgattgaac attcttgtca
     3721 ggtgaagcgc aagatggtgg ttgaagatga gttggacaat ggtattcgtc tttacctcaa
     3781 ttttggccac actattggcc atgccatcga agcgactgcc ggttatggca aggtcatgca
     3841 tggagaggct gttgccatgg gaatggtaca gatttccaag attgctgagg aaaaaggcct
     3901 catgccagct ggcattaccc aatctatcac agagatgtgt cagaaattcg gcttgcctgt
     3961 tgactatgaa aattgggaag ttgacaagct ttatcaggct cttactcatg acaagaaagc
     4021 gcgtggtaac accttgaaat tggtcttggt gccagagctt ggttcagcga ccattcatcc
     4081 agtttctctg gaagagatga aagactactt ggtaaaataa ggagagtgta tgagatattt
     4141 aactgcagga gaatcacacg gcccccgtct aacagctatt attgagggaa ttccagctgg
     4201 acttccattg acagctgagg atatcaatga ggaccttaga cgccgtcagg gtggctacgg
     4261 tcgtggtggt cgtatgaaga ttgagagtga ccaggttgtc tttacttcgg gcgttcgcca
     4321 cgggaagacg acaggggcgc ctattactat ggatgtcatc aataaggacc accagaaatg
     4381 gctggacatc atgtctgcgg aggacattga agaccgcctt aaaagcaagc ggaaaattac
     4441 tcatcctcgc ccaggtcatg ccgatttggt tggggggatt aagtaccgtt ttgatgattt
     4501 gcgaaattct ttggagcgtt catcagctcg tgaaaccacc atgcgggtgg cagttggtgc
     4561 agtagccaaa cgtctcttgg ctgagctgga tatggagatt gccaaccatg tcgtggtctt
     4621 tggtggcaag gaaatcgatg ttcctgaaaa tctgacagtc gctgaaatta agcaaagagc
     4681 tgcccagtct gaagtttcta ttgtcaacca agaacgagaa caggaaatca aggactatat
     4741 tgaccaaatc aaacgtgatg gtgataccat cggtggggtt gtggagacag tcgtcggagg
     4801 cgttccagtt ggtcttggtt cctatgtcca atgggataga aaattggatg caagattggc
     4861 tcaagctgtt gtctctatca atgcctttaa aggggtggaa tttggtcttg gctttgaggc
     4921 tggttatcgt aaaggcagcc aagttatgga tgaaattctc tggtctaaag aagacggtta
     4981 tactcgccgt accaataatc taggtggttt tgaaggtggt atgactaatg ggcaacccat
     5041 cgttgttcgt ggggtcatga aacccattcc tactctttat aaacctctta tgagtgtgga
     5101 tatcgaaacc cacgaacctt acaaggcaac cgtggagaga agtgatccga ctgctcttcc
     5161 agctgcagga atggtcatgg aagcagttgt agcaacggtt ctggcgcaag aaatcctcga
     5221 aaaattctca tcagataatc ttgaggaact aaaagaagcg gtagccaaac accgagacta
     5281 tacaaagaac tattaaggag ttcctatggc aaaaacaatc tatatcgcag gtcttgggtt
     5341 gattggagcc tctatggcac ttggtatcaa acgcgatcat ccagattatg aaattttagg
     5401 ttataatcgt agtcaagctt cgagagatat cgccttgaaa gaaggtatga ttgaccgtgc
     5461 aacggatgat tttgctagtt ttgctccttt ggcagatgtc attatcctca gcttgccaat
     5521 caaacaaact attgctttca ttaaggagtt ggccaatttg gatttgcgag aaggcgttat
     5581 tatttcagat gctggttcga ccaagtcaac cattgtggat gcggcggagc agtatttggc
     5641 tggcaagtct gttcgctttg tcggggccca tcccatggct ggtagtcaca agacaggggc
     5701 tgcttcggca gatgtcaatc tttttgaaaa tgcctattat atctttacac cttcaagcct
     5761 gacaagtcag gacacgctta aggaaatgaa ggatctgctt tcaggtcttc atgctcgttt
     5821 tatcgagatt gatgccaagg agcatgatcg tgtcacttct cagattagcc attttcctca
     5881 tattttggct tctagtctca tggagcagac tgcggtctat gctcaagagc atgagatggc
     5941 aaggcgcttt gcggcaggtg gttttcgaga tatgacccga attgcggaaa gcgagccagg
     6001 aatgtggacc tccattctct tgtccaatag cgagaccatt ctggatagaa ttgaggattt
     6061 caaggaacgt ttggaagcga ttggtcaggc cattagtaag ggagatgaag agcaaatttg
     6121 gaactttttt aaccaagcgc gtgaacaacg ccagaccatg gaaatccata agcgtggtgg
     6181 tgtggatagc tcttacgacc tctatgttga cgttcctgat gaagaagatg tcatcctgcg
     6241 gattttggaa ctgctacgtg gaacttcctt ggttaacatc cacatcaacg aggaaaatcg
     6301 tgaggatatt cacggaatcc tacaaatttc atttaaaaat gctcaagact tggaaagagc
     6361 tgagcatctc ataacagaaa ataccgacta tacagtcgtc atcaaataag gagaagaaca
     6421 tgtcaaatat ttacgatagt gcaaacgaac tcagtcgcgg tctacgcgga ttaccagaat
     6481 acaaggctgt taaagcagct aaagatgcga ttgcagcaga tgctgaggca agcaaaatct
     6541 ttacagaata tcttgccttt caagaggaaa ttcaaaaact agcacagaca ggtcaaatgc
     6601 cagatgcgtc cttccaagct aagatggaag gctttggtaa acagattcaa ggaaatagcc
     6661 tcttgtcaga attctttacc aagcaacaac aattggccat ttacctttct gacattgaaa
     6721 aaattgtttt tgaaccagtt tcagaattgc tgaaataaga aaagaacgat cctaaagtca
     6781 ggaattttta aggaatttct ggctttttat gataaaatag gtaaaagtat tgcaagtatg
     6841 aggtccagta tgaaactaaa aacaaacatt cgccatttac atggtagtat ccgcgtccca
     6901 ggtgacaagt ctatcagcca ccgttccatt atctttggaa gtttggctga gggtgagacc
     6961 aaggtttatg atattctgcg aggtgaagac gttctttcga ccatgcaggt ttttcgtgac
     7021 cttggtgttg aaattgagga taaagatggg gttattacca ttcaaggtgt aggcatggct
     7081 ggcttaaaag cgccacaaaa tgcccttaat atgggaaatt ctggcacctc gattcgcctg
     7141 atttcaggtg tccttgctgg tgcagatttc gaagtagaga tgtttggaga tgatagtctt
     7201 tccaaacgtc ctatggaccg tgtgaccctt ccactgaaaa aaatgggcgt cagcatttca
     7261 gggcaaactg aacgagactt gcctcccctt cgcttaaaag ggacgaaaaa cctaagacct
     7321 attcattatg agttgccaat tgcctctgcc caagtcaagt cagccttgat gtttgcagcc
     7381 ttacaagcta agggggagtc agttattatc gaaaaagagt gcacccgtaa tcatactgaa
     7441 gatatgttga aacaatttgg tggtcattta agtgtggacg gtaagaaaat cacagtccaa
     7501 gggccacaaa aattgacagg acagaaggtg gtcgtaccag gagatatttc cagtgcagcc
     7561 ttttggttag tcgcaggttt gattaatcca aattctcatc tagtgctgca gaatgtggga
     7621 atcaacgaaa cgcgtactgg tattattgat gtcattcgtg ccatgggtgg aaaattagaa
     7681 gttactgaaa tcgatccagt cgctaaatct tctaccttga ctgttgagtc ttctgacttg
     7741 aaaggaacag aaattggtgg cgctttgatt ccacgattga ttgatgaatt gcccattatt
     7801 gccctacttg cgacccaagc ccaaggtgta acagttatca aggatgctga ggagctcaag
     7861 gtcaaggaaa cagaccgtat tcaggttgtg gcagacgcct taaatagtat gggagcagat
     7921 attactccta cggcagatgg gatgattatc aaaggaaaat cagctcttca cggtgctaga
     7981 gtcaatacgt ttggtgacca ccgtatcggc atgatgacag ctatcgcagc cctcttggtt
     8041 gcagatggag aggtggagct tgaccgtgca gaagccatca ataccagcta tcctagtttc
     8101 tttgatgatt tggagagctt gattcatggc taaggtgtta ttagggttta tgggggctgg
     8161 aaaatcgact attgcaagag gcttggaccc taattacctt gatatggatg ctctgattga
     8221 gaatcgccta ggtatgtcca ttgcgaattt tttcgctgaa aagggagaaa cagcctttcg
     8281 tcaggtagaa tcagaagtcc tagctgattt actacaaaca gaccaagtcg tgtcaactgg
     8341 aggaggagtg gttatttctc agagaaatcg tgacttactc aagactaata cagataacat
     8401 ctacctgaga gcagattttg aaaccctcta ccaacgtatc gcagctgata aggacaatca
     8461 gcgaccgctt tttctaaata atagcaagga agaactagta gctattttcc aagaaagaca
     8521 ggcttggtat gaggaagtgg ctagtcgggt tttggatgtg accaagctaa gcccagagga
     8581 aattatagag gaactaagat gaaaattgct tatctaggtc ccaagggatc attttcacac
     8641 cacgttgtgc agacagcttt tcctcatgag gaattgcagg cttttgccaa cattacagat
     8701 gtcatcaagg cttatgagca aggattagtg aactattctg tggtgccagt tgaaaattct
     8761 attgagggta gtgttcatga aaccttggac tatctttttc atcaggctca catccaagca
     8821 gtagcagaaa tcgttcagcc tattcatcag cagttgatgg tggttccagg ccatactaag
     8881 attgaaaaga ttttttcaca tccacaggcc ttggctcaag gaaagaaatt catcgatgaa
     8941 caatatccag aggctcaaat cgaggtaaca gctagtacag cttatgcggc ccgttttatt
     9001 tccgaacatc cagaccagcc ctttgcagca gttgcaccta gaagttctgc tgaagaatat
     9061 ggattggaac tgattgccga ggatattcag gaaatggaag ccaatttcac acgtttctgg
     9121 gttctaggag ctgaaaagcc tagtattccc ttgcaagcac aaactgaaaa gatgagtttg
     9181 gccttgacat tacctgacaa ccttccaggt gcactttata aggccctgtc gacctttgct
     9241 tggcgaggaa ttgacttgac aaaaattgaa agttgtccac tcaagacagc actgggtgaa
     9301 tactttttca ttatcgatgt ggattatacc gataaggact tggtccactt tgcccaaaaa
     9361 gaattagaag cgattggaat ccagtataaa attctgggtg cctatcctat ttatccaata
     9421 tcagaccatg gaaaggagag aagatgagta aagaaaatcc cttaagtcat catgagcagt
     9481 tgcgttatga ttatttgcta aaaaatattc actatctcaa tgagagagaa aaaaatgagt
     9541 ttgcctattt gcaagaaaag ctaactcttg ctaggggaaa tagtagctct agcttggaac
     9601 aagaaagaga agagcaggtt gacttaccaa gctatgcgaa ccggagtcgc tcacaatcca
     9661 aatcacaagc actctctttc cctccaaaaa agaaaagacg gaagctccgt cttaaacgaa
     9721 tttttatggt gattttttca cttttggtct gtgtggcttt ggccatggta ttcatgtttt
     9781 tgcgtggtta ccaagatgct agcgcaaaga aaactgctga tgcccgggca gctcaagtag
     9841 aagtctttaa tggtcaggac actagagatg gagttaatat tttaatcatg ggtactgatg
     9901 gtcgaatcgg ccagaacagt gttgagacac gaactgactc tattatggta ttaaatgtcg
     9961 ggggctcaga taagaaaatg aagctagtca gtttcatgcg tgataatttg gtctatatag
    10021 atggttatag tcaagtgatt aatggtagaa aacagacaga taacaagtta aacgtagcct
    10081 acgagttagg agaacaagag gggcaaaaag gggcagaaat ggttcgccaa gtcttgaaag
    10141 ataattttga cttggacatt aagtactacg ccttggttga tttccaggcc tttgccacag
    10201 cgattgacac gcttttccct gatggggtga cgattgatgc tcaattttca acattgaatg
    10261 ggcgtcccct aacagaagct acagtcggag atgatttata cgctaccgag actgagtctc
    10321 caacccaaac cattaaagtc ggaaaacagc agatgaatgg ctcgaccctg ctcaattatg
    10381 ctcgtttccg tgatgatgat gaggcagatt acggccgtac aaaaagacag caacaagttt
    10441 taacagcaat tttagagcaa attaaagatc ccactaaact tttcactggt tctgaagctc
    10501 ttggaaaggt ttttgctatg acctcaacca acgtacccta tactttcctc ctaacaaacg
    10561 gtttatctgt tttggatgga gcaaaaaatg gtattgaaaa attgacgatt ccagaactgg
    10621 gtgactgggt agatgcctat gatgtttatg ggggcttggg cctgctggtt gatcaaaaca
    10681 aatatcaaac caagctcgct caaatgggct taagataaga taatactcaa tgaaaatcaa
    10741 agtgcaaact aggaagctag ccgcaggctg ctaaaaacac agttttgagg ttgtagataa
    10801 gactgacgaa gtcagttacc tatacctacg gcaaggtgaa gctgacgtgg tttgaagaga
    10861 ttttcgaaga gtataagaca gaaaaatcaa agtttgatag ctatttggat agcagtcagg
    10921 ctttgatttt ttacagtctg caatagattt tcaccccttg tttgtggtat aatgaaacga
    10981 tacgatagaa agaataatga acaatatgac tgatttaaaa gcaattcagg ctcgtagtct
    11041 ggagatggct gaatattttg tggccttttg taaagaacat gatttgctct gttatctctg
    11101 tggcggaggc gctattggtg cccttcgaaa caagggattt attccttggg acgacgacct
    11161 agactttttt atgcctcgta aagattatga gaaattagca gaattatggc ctcgttatgc
    11221 agatgaacgt tatttcttgt caaagagtca caaggatttt gttgatcgta atctttttat
    11281 taccattcgt gacaagaaaa ccacctgtat caagccttat cagcaggatt tggatttgcc
    11341 acatggtctg gccttggatg ttttgccttt ggattattat ccgaaaaatc cagctgagcg
    11401 gaaaaaacag gttcgttggg ccttgattta ttcactcttt tgtgcgcaaa ctattccaga
    11461 aaagcatggt gatctcatga aatggggaag tcgcatttta ctgggtttga ctccaaaatc
    11521 tctccgttat cgcatctgga aaaaagctga gaaagaaatg actaagtatg atttggctga
    11581 ttgtgatggc attacagaat tatgctcagg tcctggctac atgagaaaca agtacccaat
    11641 cacatctttt gaagacaatc ttttcttgcc atttgaagga acagagatgc ctattccaat
    11701 cggctatgat gtctatctca gaactgcttt tggggattat atgacgcctc caccagcaga
    11761 caagcaggta ccgcatcatg atgctgtcat cgctgatatg gataagtctt atacagaata
    11821 caagggagaa tatggtggct aagaaaaaaa tcttattttt tatgtggtct ttttctcttg
    11881 gaggtggtgc agagaagatt ctatcaacca ttgtttcaaa tctggatcca gaaaagtatg
    11941 atattgatat tcttgaaatg gagcactttg acaagggata tgaatctgtt ccaaagcatg
    12001 tacgcatttt aaaatccctt caagattatc gccaaaccag atggttacga gcttttttgt
    12061 ggagaatgag aatttatttt ccaagactga ctcgtcgttt gcttgtaaaa gatgattatg
    12121 atgttgaagt ttcttttacc attatgaatc caccactgtt gttctctaaa agaagagaag
    12181 tcaagaagat atcttggatt catggaagta ttgaagaact tcttaaggat agctctaaaa
    12241 gagaatcaca tagaagccag ttggatgctg cgaatacaat tgtagggatt tcaaaaaaga
    12301 ccagcaattc tatcaaggaa gtttatccag attatacttc taaattacag acaatctaca
    12361 atggatatga ttttcagact attctagaaa aatctcaaga gaagatcgat atcgagattg
    12421 ctcctcaaag tatctgtact atcggacgga ttgaggaaaa taagggttct gaccgtgtag
    12481 tggaagtgat acgattatta caccaagagg gaaaaaacta tcatctctat tttatcgggg
    12541 ctggtgatat ggaagaggaa ctgaaaaaac gagtcaaaga gtatgggatt gaggactatg
    12601 tacatttcct tggttatcaa aaaaatcctt atcagtatct atctcagacg aaagttcttt
    12661 tgtctatgtc taaacaagaa ggttttcctg gagtgtatgt ggaggccttg agtctgggac
    12721 tcccttttat ctctacggac gttggagggg ctgaggaatt atcccaagaa ggacgatttg
    12781 gacaaatcat tgagagcaat caagaggcag ctcaggcgat tactaattac atgacttctg
    12841 cctcaaactt tgatgtcgat gaggctagcc aattcattca acaatttaca attacaaaac
    12901 aaatcgaaca agtagaaaaa ctattagagg agtagcatgg aaactgcatt aattagtgtg
    12961 attgtgccag tctataatgt ggcgcagtac ctagaaaaat cgatagcttc cattcagaag
    13021 cagacctatc aaaatctgga aattattctt gttgatgatg gtgcaacaga tgaaagtggt
    13081 cgcttgtgtg attcaatcgc tgaacaagat gacagggtgt cagtgcttca taaaaagaac
    13141 gaaggattgt cgcaagcacg aaatgatggg atgaagcagg ctcacgggga ttatctgatt
    13201 tttattgact cagatgatta tatccatcca gaaatgattc agagcttata tgagcaatta
    13261 gttcaagaag atgcggatgt ttcgagctgt ggtgtcatga atgtctatgc taatgatgaa
    13321 agcccacagt cagccaatca ggatgactat tttgtctgtg attctcaaac atttctaaag
    13381 gaatacctca taggtgaaaa aatacctggg acgatttgca ataagctaat caagagacag
    13441 attgcaactg ccctatcctt tcctaagggg ttgatttacg aagatgccta ttaccatttt
    13501 gatttaatca agttggccaa gaagtatgtg gttaatacta aaccctatta ttactatttc
    13561 catagagggg atagtattac gaccaaaccc tatgcagaga aggatttagc ctatattgat
    13621 atctaccaaa agttttataa tgaagttgtg aaaaactatc ctgacttgaa agaggtcgct
    13681 tttttcagat tggcctatgc ccacttcttt attctggata agatgttgct agatgatcag
    13741 tataaacagt ttgaagccta ttctcagatt catcgttttt taaaaggcca tgcctttgct
    13801 atttctagga atccaatttt ccgtaagggg agaagaatta gtgctttggc cctattcata
    13861 aatatttcct tatatcgatt cttattactg aaaaatattg aaaaatctaa aaaattacat
    13921 tagaacgggg gtgaccaagt gattgatggg aaacgattat tatttagttt gaccatagtc
    13981 agttatgcct tgacgctagt aagtggaatt gtgtatctgt ttaataataa taatgttagc
    14041 ttactttcta ctttattgtt cttgttggtt agtagcttaa ttgcttgttg gaatgatatc
    14101 aagtattact taatccattt tattttctat ttaaccattt ttgtatttct ggtatcaaga
    14161 ccgaccattg attattttag ggatggtgct ttggatacct atcatccaat agcctatcgt
    14221 tttgccttta tagttgtcat gatttcgatt ctgggcttga ccacaggagg cattctggct
    14281 cgttacttca tagctaggaa gaaaataaaa gtagcaaata taggaaattc tctaaaagag
    14341 gtttatatca agcggttacg ctttgtatca ctaggagttt ttcttctaac ttatcctttc
    14401 tatttcattc ggttatttga acggctcttg tatcgtttgc agacttccta ctatgcctac
    14461 tatgcaaatt ttgaaagtaa actgccttat tttacctaca ttttgtctac ctttacggtc
    14521 tatgcaatgt gtatgtatct ggcaaccaag ccaaagaaat tgcaggccac agcagtgctt
    14581 gtctccttta ttgcagctaa tactattcat ttggcaattg ggacacgaaa tccctttatt
    14641 ttaagtattt tatttgcttt tgtttattac tttatgcggg agcaaactga aaaaggaaaa
    14701 tggattgggt ttaaagaaaa gttagcgatt tttgtaggtt ctcctattct catgttagcg
    14761 atgggagtac tcaattatgt acgggataat gtccaagttt cccatacagg tttctgggat
    14821 atcttacttg actttatcta taaacaaggg actagttttg gtgttctggc tcgaggtttt
    14881 ctatttaaca gtagcctccc ttaccgagat ttccgtaatt ttacttttgg tcctgttctt
    14941 gattattttg caagggggag tttgggagcc attttcggag gaaaagcctt tgaacataca
    15001 accaatagtg tggaactagc tattgatagt aatagttatg cccacaatct atcctatctt
    15061 gtcttgaaca aggaatactt gaaagggcat ggtatcggaa gtagttatat catggagttg
    15121 tataccgact atggtatgat tggagtcttt ctgcttagtt tcttactcgg cgtattattt
    15181 atagccatgc tgcaagtagc ctatcgctca aggacaatcc tatttgcttt atccctactc
    15241 atcttgaata atctattctt tatgccaaga agcagctttt cagaaagttt cttcaattta
    15301 tttacaatgc aattctgggg aattgttctt gtgattatat ttgtagcaaa aatgcttaca
    15361 aaggaaaact agtatctact aaacaaagga gaaaaaaatc atgtttgaac attattcagt
    15421 agctgatttg tttgcaaatc tttataaaaa gcgaaaagca aatattttag ctctcatcgc
    15481 tttatttgct ctcattgctg taccatttac aattaaagca gttaggaata aaaacactgt
    15541 caaagataca acaagttatt caacttatct tatctataaa atcactcctc caaaagagtc
    15601 ggccaaaacg attttgaatc atcaaattgg tggttatagt gatttttatg ggaaattgat
    15661 tgatggtaat ttgaatggag cttatctttt caatgatgta gaacccagtg agttgaaaaa
    15721 aattgccagt gaattagaca cgacagaaac aaccttgaaa aattcaacga atgactattg
    15781 gtggaaaaaa ttgaccgtct actatatgat tgacgatgca ggggttggtg tgaaaatttt
    15841 gacatcaagt aaagatgcca ataacttgtt agagaaaaaa attgatgggt tgattgagaa
    15901 atttaaacat gcctatgcaa atgtgaaaat tgaaaaactg gaaaccatca actctaaaga
    15961 attgaacgca aatggtgaaa cagcgcttgg cttaaatgtg aaaaatctga ttcttcgttt
    16021 agttgttatt ggagtggttt gtgtgatttt ggttgtgatg ggaaatgtgt tagtttatct
    16081 ctttaatcca acaatcaata gagtaggtga tttttctcag tatcaaattg attttgtaac
    16141 agagattaca acaattgcta acctagcaga tgttttgtca tacaaaaata ctggacagga
    16201 attgaccatc gttagctcaa ataaagctat cttagataaa ttgaaacaga gtcaagaagc
    16261 tttaaaagga atgcattttg tagatttaca ggatgtatca tctcttttgg aaagagatac
    16321 agtccttctt gttgaagagt acggagtgac tcgttataag aaatttgagc aaagtcttca
    16381 aattctcaga aacttaaatc gttctatcct cggtgtagca acctttaaat tataagtatt
    16441 ctgatgtata aggtcttcaa agtcggctct tagccgattt tgaaggcttt ttctatctgt
    16501 ctgcagaaat tttcttttgg agtctcctat agaaagaccc tagggaaaat ctatccgtat
    16561 agaggagcct tcttttgata aaatcgtaaa aatctagtat aatagataga actgaaagta
    16621 tgaggttact agcaatgaaa atgaaacaaa ttagtgatac aactttaaaa atcacgatat
    16681 ctttagagga tttgatggat cgtggaatgg agattgctga ctttctcgtt cctcaagaaa
    16741 aaacagaaga gttcttttat gctatcttgg atgagctaga gatgcctgat agctttctgg
    16801 atacaggcat gttgagcttc cgtgtgactc caaaacctga taaggtagat gtctttgtga
    16861 ccaagtcaaa gattgaccaa aatctagatt ttgaagactt atcggatttg ccagatatgg
    16921 aagaattggc tcaaatgtct ccagatgaat ttatcaaaac cctggaaaaa agcatcgcag
    16981 acaaaaccaa ggatgatatc gaagccattc aatctcttga gcaagttgaa gtcaaggaag
    17041 aagagcaaga gcaggctgaa caagaagctg agagtaagaa agaaccttac atctactaca
    17101 tcctttcttt tgctaagttg gctgacttgg tagcttttgc caagacagtg acttttgaaa
    17161 tggaaacttc tgaactctac aaaatgaacg agcgctatta tttgaccatt ttagtggata
    17221 ttgaaaatca tccaagccca tatccagctt ggctgttggc ccgtatgcgc gagtttgcag
    17281 acgatagtga tatcagtcgc tcagtcttac aagagtatgg tcaagtcttg atgagtcacg
    17341 atgcagtgct caatctgcaa aaaatcggct aatcttttct ggaaagctat tttcagattt
    17401 taagaagagc gaatcgtctg attcgttttt tctttttata ctgaaatagt gatttactat
    17461 aataggaatt ttcacaaaat tctgttataa tggctatatt agaaaatttc gaggagataa
    17521 agatgacagt taaaattgct ttactaggat ttggtaccgt tgcaagtggt gtgcctttcc
    17581 tcctaaagga aaatggagga aaaatcaatc aatcagcaca ttcagatatc aaagttgcta
    17641 aggtattggt caaggatgaa gatgaaaaaa atcgcttgct tgcagcaggg aatgacttta
    17701 actttgtaac caatgtggat gatattttat cagaccagga tattactatc gtagtggaat
    17761 tgatggggcg tattgagcct gctaaaacct ttatcactcg tgccttggaa gctggaaaac
    17821 acgttgttac tgctaacaag gaccttttag ctgtccatgg cgcagaattg ctagaaatcg
    17881 ctcaagctaa caaggtagca ctttactacg aagcagcagt tgctggtggg attccaattc
    17941 ttcgtacttt agcaaattcc ttggcttctg ataaaattac gcgcgtgctt ggagtagtca
    18001 acggaacttc caacttcatg gtgaccaaga tggtggaaga aggctggtct tacgatgatg
    18061 ctcttgcgga agcacaacgt ctaggatttg cagaaagcga tccgacgaat gacgtagatg
    18121 ggattgatgc agcctacaag atggttattt tgagccaatt tgcctttggc atgaagattg
    18181 cctttgatga tgtagcccac aagggaatcc gcaatatcac accagaagac gtagctgtag
    18241 ctcaagagct tggttacgta gtgaaattgg ttggttctat tgaggaaact tcttcaggta
    18301 ttgctgcaga agtgactcca accttcctac ctaaagcgca cccacttgct agtgtgaatg
    18361 gcgtaatgaa cgctgtcttt gtagaatcta tcggtattgg tgagtctatg tactacggac
    18421 caggtgcggg tcaaaaacca actgcaacaa gtgttgtagc tgatattgtc cgtatcgttc
    18481 gtcgtttgaa tgatggtact attggcaaag acttcaacga atatagccgt gacttggtct
    18541 tggcaaatcc tgaagatgtc aaagcaaact actatttctc aatcttggct ctagactcaa
    18601 aaggtcaggt cttgaagttg gctgaaatct tcaatgctca agatatttcc tttaagcaaa
    18661 tccttcaaga tggcaaagag ggtgacaagg cgcgtgtcgt tatcatcaca cacaagatta
    18721 ataaagccca gcttgaaaat gtctcagctg aattgaagaa ggtttcagaa ttcgacctct
    18781 tgaatacctt caaggtgcta ggagaataag atgaagatta ttgtacctgc aaccagtgcc
    18841 aatatcgggc caggttttga ctcggtcggt gtagctgtaa ccaagtatct tcaaattgag
    18901 gtctgcgaag aacgagatga gtggctgatt gaacaccaga ttggcaaatg gattccacat
    18961 gatgagcgta atctcttgct caaaatcgct ttgcaaattg taccagactt gcaaccaaga
    19021 cgcttgaaaa tgaccagtga tgtccctttg gcgcgcggtt tgggttcttc cagctcggtt
    19081 atcgttgctg ggattgaact agccaaccaa ctgggtcaac tcaacttatc agaccatgaa
    19141 aaattgcagt tagcgaccaa gattgaaggg catcctgaca atgtggctcc agccatttat
    19201 ggtaatctcg ttattgcaag ttctgttgaa gggcaagtct ctgctatcgt agcagacttt
    19261 ccagagtgtg attttctagc ttacattcca aactatgaat tacgtactcg cgacagccgt
    19321 agtgtcttgc ctaaaaaatt gtcttataag gaagctgttg ctgcaagttc tatcgccaat
    19381 gtagcggttg ctgccttgtt ggcaggagac atggtgaccg ctgggcaagc aatcgaggga
    19441 gacctcttcc atgagcgcta tcgtcaggac ttggtaagag aatttgcgat gattaagcaa
    19501 gtgaccaaag aaaatggggc ctatgcaacc tacctttctg gtgctgggcc gacagttatg
    19561 gttctggctt ctcatgacaa gatgccaaca attaaggcag aattggaaaa gcaacctttc
    19621 aaaggaaaac tgcatgactt gagagttgat acccaaggtg tccgtgtaga agcaaaataa
    19681 agaatagaag ataggatggg gaaactcttg accagagggt ttcatatcct ttttgtgaaa
    19741 agaagtttat actcaatgaa aatcaaagag caaactagga agctagccgc aggctgctca
    19801 aaacagtgtt ttgaggttgt ggatagaact gaagaagtca gctcaagaca ctgttttgag
    19861 gttgcagata gaactgacga agtcagtaac atatatacgg taaggtgacg ctgacgtggt
    19921 ttgaagagat tttcgaagag tattagttaa aaacgtgata aaggagaaat aaagatggca
    19981 gaaatttatc tagcaggtgg ttgtttttgg ggcctagagg aatatttttc acgcatttct
    20041 ggagtgctag aaaccagtgt tggctacgct aatggtcaag tcgaaacgac caattaccag
    20101 ttgatcaagg aaacagacca tgcagaaacg gtccaagtga tttacgatga gaaggaagtg
    20161 tcactcagag agattttact ttattatttc cgagttatcg atcctctatc tatcaatcaa
    20221 caagggaatg accgtggtcg ccaatatcga actgggattt attatcagga tgaagcagat
    20281 ttgccagcta tctacacagt ggtacaggag caggaacgca tgctgggtcg aaagattgca
    20341 gtagaagtgg agcaattacg ccactacatt ctggctgaag actaccacca agattatctc
    20401 aggaagaatc cttcaggtta ctgtcatatc gatgtgaccg atgctgataa gccattgatt
    20461 gatgcagcaa actatgaaaa gcctagtcaa gaggtgttga aggccagtct atctgaagag
    20521 tcttatcgtg tcacacaaga agctgctaca gaggctccgt ttaccaatgc ctatgaccaa
    20581 acctttgaag aggggattta tgtagatatt acgacaggtg agccactctt ttttgccaag
    20641 gataagtttg cttcaggttg tggttggcca agttttagcc gtccgatttc caaagagttg
    20701 attcattatt acaaggatct gagccatgga atggagcgaa ttgaagttcg ttctcgttca
    20761 ggcagtgctc acttgggtca tgttttcaca gatggaccgc gggagttagg cggcctccgt
    20821 tactgtatca attctgcttc tttacgcttt gtggccaagg atgagatgga aaaagcagga
    20881 tatggctatc tattgcctta cttaaacaaa taaaacagag agtggggctt cccactttct
    20941 tcatttctag aatatgaata gaagggattt atgaaacacc tattatctta cttcaaaccc
    21001 tacatcaagg aatcaatttt agcccccttg ttcaagctgt tagaagctgt ttttgagctc
    21061 ttggttccca tggtgattgc tgggattgtt gaccaatctt tacctcaggg agatcaaggt
    21121 catctctgga tgcagattgg cctgctcctt atctttgcag taattggcgt tttagtggcc
    21181 ttgatagctc aattttactc agcaaaggca gcagtaggat ttgctaagga attgacaaac
    21241 gatctttatc gtcatattct ttccttgcca aaggacagca gagaccgtct gacaacttct
    21301 agtttggtta ctcgcttgac ttcggatacc taccagattc agactggtat caatcaattc
    21361 ctgcgtctct ttttacgagc gcccattatc gtttttggtg ccatttttat ggcttatcga
    21421 atctcagctg agttgacttt ctggttctta gtcatggttg ccattttgac cattgtcatt
    21481 gtagggttat ctcgattggt caatcctctc tacagtagtc tcagaaagaa aacggaccaa
    21541 ctggttcagg aaacgcgcca gcaattgcaa gggatgcggg ttattcgtgc ttttggtcaa
    21601 gaaaaacgag agttacagat ttttcaaacc cttaaccaag tttatgctag attacaagaa
    21661 aagacaggtt tctggtctag tttattaaca cctctgacct atctgattgt caatggaact
    21721 cttctcgtta ttatctggca aggctatatt tcaattcaag gaggagtgct cagtcaaggt
    21781 gctctcattg ctcttatcaa ttacctctta cagattttgg tggaattggt caagctagcc
    21841 atgttgataa attctctcaa ccagtcctat atctcagcca agcgaatcga ggaggtcttt
    21901 gcggaatctc ccgaaaacat ccattcagaa ttagaacaaa agcaagttac cagtggtcgg
    21961 gttttacaag tccaagaatt gacttttacc tatcctgatg cggcccagcc ttctctgaga
    22021 tacatttcct ttgatatgac tcaaggacaa attctaggta tcatcggggg aacgggttct
    22081 ggtaagtcaa gcttggtgca actcttactt ggactttatc caatagataa ggggagcatt
    22141 gacctttatc gagatggatc tagtcctcgt aatttagagc agtggcggtc ttggattgct
    22201 tatgttcccc aaaaggtcga actttttaag ggaactattc gttccaactt gactttaggt
    22261 ttaaatcaag aagtgactga tcaagaactc tggcaagcct tggaaattgc tcaagctaag
    22321 gattttgtca gtgataagga aggactcttg gatgctctag ttgaagcagg agggcgaaat
    22381 ttctcaggtg gacaaaaaca aaggctgtct atcgcacgag cagtcttgcg ccaggctccg
    22441 tttctcatcc tagatgatgc gacctcagca ctggacacca ttaccgagtc caagctcttg
    22501 aaagctatta gagaaaattt tccaaacatg agcttaattt tgatctctca acgaacctcg
    22561 actttacaga tggctgacca gattctcctc ttggaaaaag gtgagttgct agctgttggc
    22621 aagcacgatg acttgatgaa atccagtcaa gtctatcgtg aaatcaatgc atcccaacat
    22681 ggaaaggagg actagaatga aacgacaaac tgtaaaccag acgctcaaac gtttagccgt
    22741 agatttagca aaccatccct tcctcctttt cctagccttt ctaggaacta ttgcccaagt
    22801 tggcttatca atttatctac ctattctgat tgggcaggtc attgaccaag tcctagtggc
    22861 tggttcatca ccagtttttt ggcagatttt tctccagatg ctcttggtgg taataggaaa
    22921 tactctagta caatgggcca atcctctcct ctataatcgt ctaatcttct cttataccag
    22981 agatttacga gagcgaatca tccataaact ccatcggtta ccgattgctt ttgcggatcg
    23041 gcaaggcaat ggagagatgg tcagtcgtgt aaccacggac atcgaacagt tggcagctgg
    23101 cttgaccatg atttttaacc aatttttcat tggtgttttg atgattttgg ttagtattct
    23161 agccatgctc caaattcatc tcctcatgac tctcttagtc ttgctgttga cgccactgtc
    23221 catggtgatt tcacgcttta ttgccaagag atcctatcat ctcttccaga agcaaacaga
    23281 gacgagggga attcagactc agttgattga agaatcgctt agccagcaga ctataatcca
    23341 gtccttcaat gctcaaacag aatttatcca aagattgcgt gaggctcatg acaactactc
    23401 aggctattct cagtcagcca tcttttattc ttcaacggtc aatccttcga ctcgctttgt
    23461 aaatgcactc atttatgccc ttttagctgg agtaggagct tatcgtatca tgatgggttc
    23521 aaccttgacc gtcggtcgtt tagtgacttt tttgaactat gttcagcaat acaccaagcc
    23581 ctttaatgat atttcttcag tgctagctga gttgcaaagt gctctggctt gcgtagagcg
    23641 tatctatgga gtcttagata gccctgaagt ggctgaaaca ggtaaggaag tcttgaccag
    23701 tgaccaagtt aagggagcta tttcctttaa acatgtctct tttggctacc atcctgaaaa
    23761 aattttgatt aaggacttgt ctatcgatat tccagctggt agtaagatag ccatcgttgg
    23821 tccgacaggt gctggaaaat caactcttat caatctcctt atgcgttttt atcccattag
    23881 ctcgggagat atcttgctgg atgggcaatc catttatgat tatacacgag catcattgag
    23941 acagcagttt ggcatggtgc ttcaagaaac ctggctcaca caagggacca ttcatgataa
    24001 tattgccttt ggcaatcctg aagccagtcg agagcaagta attgctgctg ccaaagcagc
    24061 taatgcagac tttttcatcc aacagttgcc acagggatac gataccaagt tggaaaatgc
    24121 tggagaatct ctctctgtcg gccaagctca gctcttgacc atagcccgag tctttctggc
    24181 tattccaaag attcttatct tagacgaggc aacttcttcc attgatacac ggacagaagt
    24241 gctggtacag gatgcctttg caaaactcat gaagggccgc acaagtttca tcattgctca
    24301 ccgtttgtca accattcagg atgcggattt aattcttgtc ttagtagatg gtgatattgt
    24361 tgaatatggt aaccatcaag aactcatgga tagaaagggt aagtattacc aaatgcaaaa
    24421 agctgcggct tttagttctg aataagccat tctcttttga aagtttatgg acgaaaaaag
    24481 ttgccttcga gtgacttttt tgttacaata gctagaaaaa ttgtttactg taatactcaa
    24541 tgaaaatcaa agagcaaact aggaagctag ccgtaggttg ctcaaaacac tgttttgagg
    24601 ttgcagatag aactgacgaa gtcagctcaa aaaactgttt tgaggttgca gatagaactg
    24661 acgaagtcag taaccacacc tacggcaaag tgaatttgaa gtggtttgaa gagagtacaa
    24721 cttgtctttt agaaaaggag cctataatga aagtctttca gcatgtaaat atcgtgactt
    24781 gtgatcaaga tttccatgtt tatcttgatg gaatcttagc agtcaaggat tctcaaatcg
    24841 tctatgtcgg tcaagataag ccagcgtttt tagagcaagc tgagcagatt atagactatc
    24901 agggagcttg gattatgcct ggtttggtca attgtcacac ccattctgca atgacaggcc
    24961 tgagagggat ccgagatgac agcaatctcc atgaatggct caatgactat atctggccag
    25021 cagaatctga gtttactccc gacatgacta ccaatgcggt caaagaagcc ctaacagaga
    25081 tgctccagtc aggaacaaca acctttaacg atatgtataa tcccaatggt gtggatatcc
    25141 agcaaattta tcaggtggtg aaaacttcca agatgcgttg ttatttttct ccgactctct
    25201 tttcttcaga gacagaaaca actgctgaga ctataagcag aactcgatcc atcatagacg
    25261 aaatcttaaa atataaaaat ccaaatttca aggttatggt agcacctcat tctccgtata
    25321 gctgcagtag agacttgctg gaagcgagtt tggaaatggc aaaagagcta aatattccgc
    25381 tccatgtcca tgtggcggag accaaggagg agtcaggaat tatcctcaaa cgatacggca
    25441 aacgccccct tgcttttctt gaagaactgg gttatttaga tcatccgtcc gtctttgctc
    25501 acggggtcga attaaacgag agagaaattg aacgcttggc atcttctcaa gtggctatcg
    25561 cccacaatcc tatcagtaac ctcaaactgg catcaggaat tgctccaatt atccagctcc
    25621 aaaaagcggg agtagcagtc ggaattgcga ctgactcggt tgcttccaat aacaatctag
    25681 atatgtttga ggaaggaagg actgcagctc ttcttcagaa gatgaaaagt ggggatgcca
    25741 gccagtttcc aatcgaaaca gctctcaagg tactgacaat cgaaggggct aaggtccttg
    25801 gaatggaaaa tcagatagga agtctggaag tcggcaagca agcagatttt ctggtcattc
    25861 aaccacaagg gaaaattcat ctccaacctc aggaaaatat gctgtctcac ctggtttatg
    25921 cagttaaatc tagtgatgta gatgatgttt atatcgccgg agaacaggtt gttaagcaag
    25981 gtcaagtcct gacagtagaa ctttaaaaga aaaattacga aaaattttaa aaaaagtttg
    26041 caaaaatctt gcattctttt tttgactatg ctatacttat atacggttta aaaaaactgc
    26101 ctaagacagt aggggagctc gactcataaa tatcctaccg aggacaaaac gtatcatgta
    26161 aaaagaagcg tattgtactt tcgtgtctag gtttgggcgc gtttttcttt ttgaaaaatt
    26221 ccccaagcaa aataattacg gaggtgaaca cactaatgag tgaagcaatt attgctaaaa
    26281 aagcggaact agttgacgta gtagctgaaa aaatgaaagc tgctgcatct atcgtcgttg
    26341 tagacgctcg tggtttgaca gttgagcaag atacagttct tcgtcgtgag cttcgtggaa
    26401 gcgaagttga gtataaagtt attaaaaact caatcttgcg tcgtgcagct gaaaaagctg
    26461 gtcttgaaga tcttgcatct gtatttgttg gaccatctgc agtagcattt tctaatgaag
    26521 atgttatcgc accagcgaaa atcttgaacg acttttctaa aaacgctgaa gcacttgaaa
    26581 ttaaaggtgg tgcaatcgaa ggcgctgtcg catctaaaga agagattctt gcacttgcaa
    26641 ctcttccaaa ccgcgaagga cttctttcta tgctcctttc tgtacttcaa gcgccagtgc
    26701 gcaacgttgc tcttgcagtc aaagcggttg cagaaagcaa agaagacgcg gcttaatctt
    26761 aagctacata gcgtagccta gctacgaaaa aaactattat aaaatttaaa acttatttgg
    26821 aggaaataac aatggcattg aacattgaaa acattattgc tgaaattaaa gaagcttcaa
    26881 tccttgaatt gaacgacctt gtaaaagcta tcgaagaaga atttggtgta actgcagctg
    26941 ctcctgtagc tgttgctgca gctgatgctg ctgatgctgg tgctgctaaa gattcattcg
    27001 acgttgaatt gacatctgca ggcgacaaaa aagttggtgt tatcaaagtt gtacgtgaaa
    27061 tcactggtct tggtcttaaa gaagctaaag aacttgttga cggtgcacca gcacttgtta
    27121 aagaaggcgt tgcaactgca gaagctgaag aaatcaaagc taaattggaa gaagctggag
    27181 cttcagttac tcttaaataa tagagtatcg caatttgatt gcggaatccc tatttaccag
    27241 tctttgagag cgataagcga ttgatagaaa ctgataaatt gatttggttt cctgccaaaa
    27301 attctttggc aggatttttg ttttaataaa ctttcggggg tagacactag attgtctacc
    27361 cctttttttg tgtttcggag gtaactatga aaagaataat tccagtttat atattccaac
    27421 aagtaaatgt cctattggta tctctatact tattgaaatt tctttgtatc ggtgagttaa
    27481 ctatactaca ggttctctat ggtgcgtctc ttttttcttt tttatggatg tatggtcaac
    27541 gaaaacaagt ggtcaaggtc aatatgaaaa ctaggatgaa atgccttggt attggactag
    27601 ctagtctact gattataagt ctatgtttta gcctgattca tgctcaagga agcacgaatc
    27661 aaacaaattt aattggcctt caacatcaag ttccttggtt ttcattttta ttgttcttaa
    27721 tcaatgcgag tatggttgaa gaatttctgt atcgtgaaat tttatggaac ttggttagaa
    27781 aattagatat tcgagttgct ttgacaagtg ttttatttgc cttagtacat catccaggaa
    27841 ccattctagc ttggtgtctg tacgtttcac ttggaatgtt tttaggtatg gtgcgctata
    27901 aatctgactt atggggaagt atggggttac atttggtgtg gaacttttta gtttatagtt
    27961 tgctgctttt ttaaaatagg tgacctgatt tatctcataa
//
LOCUS       DAVHEW010000014        26830 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVHEW010000014 DAVHEW010000000
VERSION     DAVHEW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.014"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..710
                     /locus_tag="VVL53_000610"
     CDS             <1..710
                     /locus_tag="VVL53_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4182819.1"
                     /translation="GRTQAIIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCA
                     KIVLDRFHIVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKRFY
                     RPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQNKKPEKFFGLIEDNLKQVH
                     PIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIF
                     IALNIKKERTKFVLSRA"
     gene            complement(782..1432)
                     /locus_tag="VVL53_000611"
     CDS             complement(782..1432)
                     /locus_tag="VVL53_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU4182820.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            1544..2386
                     /locus_tag="VVL53_000612"
     CDS             1544..2386
                     /locus_tag="VVL53_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU4182821.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            2462..2899
                     /locus_tag="VVL53_000613"
     CDS             2462..2899
                     /locus_tag="VVL53_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU4182822.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            2968..3271
                     /locus_tag="VVL53_000614"
                     /pseudo
     CDS             2968..3271
                     /locus_tag="VVL53_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3362..4435)
                     /locus_tag="VVL53_000615"
     CDS             complement(3362..4435)
                     /locus_tag="VVL53_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU4182823.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVTTVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     EKETLGRLTQLLIDTRYQVWKNR"
     gene            4591..5268
                     /locus_tag="VVL53_000616"
     CDS             4591..5268
                     /locus_tag="VVL53_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4182824.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            5261..6235
                     /locus_tag="VVL53_000617"
     CDS             5261..6235
                     /locus_tag="VVL53_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU4182825.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6284..8227)
                     /gene="thrS"
                     /locus_tag="VVL53_000618"
     CDS             complement(6284..8227)
                     /gene="thrS"
                     /locus_tag="VVL53_000618"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU4182826.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(8285..8608)
                     /locus_tag="VVL53_000619"
     CDS             complement(8285..8608)
                     /locus_tag="VVL53_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU4182827.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(9039..9239)
                     /locus_tag="VVL53_000620"
     CDS             complement(9039..9239)
                     /locus_tag="VVL53_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182828.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            9477..10106
                     /locus_tag="VVL53_000621"
     CDS             9477..10106
                     /locus_tag="VVL53_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4182829.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            10247..10516
                     /gene="rpsO"
                     /locus_tag="VVL53_000622"
     CDS             10247..10516
                     /gene="rpsO"
                     /locus_tag="VVL53_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU4182830.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            11331..11945
                     /locus_tag="VVL53_000623"
     CDS             11331..11945
                     /locus_tag="VVL53_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU4182831.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(12237..12986)
                     /locus_tag="VVL53_000624"
     CDS             complement(12237..12986)
                     /locus_tag="VVL53_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU4182832.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            13119..15455
                     /locus_tag="VVL53_000625"
     CDS             13119..15455
                     /locus_tag="VVL53_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU4182833.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLIRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            15557..16234
                     /locus_tag="VVL53_000626"
     CDS             15557..16234
                     /locus_tag="VVL53_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU4182834.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            16221..17018
                     /locus_tag="VVL53_000627"
     CDS             16221..17018
                     /locus_tag="VVL53_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU4182835.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            17035..18138
                     /locus_tag="VVL53_000628"
     CDS             17035..18138
                     /locus_tag="VVL53_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4182836.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            18145..18336
                     /locus_tag="VVL53_000629"
     CDS             18145..18336
                     /locus_tag="VVL53_000629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182837.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            18439..19458
                     /gene="galE"
                     /locus_tag="VVL53_000630"
     CDS             18439..19458
                     /gene="galE"
                     /locus_tag="VVL53_000630"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU4182838.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            19460..20425
                     /locus_tag="VVL53_000631"
     CDS             19460..20425
                     /locus_tag="VVL53_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4182839.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(20476..20682)
                     /locus_tag="VVL53_000632"
     CDS             complement(20476..20682)
                     /locus_tag="VVL53_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU4182840.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            20734..21210
                     /locus_tag="VVL53_000633"
     CDS             20734..21210
                     /locus_tag="VVL53_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU4182841.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            21219..21890
                     /locus_tag="VVL53_000634"
     CDS             21219..21890
                     /locus_tag="VVL53_000634"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU4182842.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPQVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            22021..22359
                     /locus_tag="VVL53_000635"
     CDS             22021..22359
                     /locus_tag="VVL53_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU4182843.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            22362..22991
                     /locus_tag="VVL53_000636"
     CDS             22362..22991
                     /locus_tag="VVL53_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU4182844.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            23231..24382
                     /locus_tag="VVL53_000637"
     CDS             23231..24382
                     /locus_tag="VVL53_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4182845.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            24700..25449
                     /gene="truA"
                     /locus_tag="VVL53_000638"
     CDS             24700..25449
                     /gene="truA"
                     /locus_tag="VVL53_000638"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU4182846.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            25439..26212
                     /locus_tag="VVL53_000639"
     CDS             25439..26212
                     /locus_tag="VVL53_000639"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU4182847.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            26190..26651
                     /locus_tag="VVL53_000640"
     CDS             26190..26651
                     /locus_tag="VVL53_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4182848.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSALMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         7591 a         5054 c         6117 g         8068 t
ORIGIN      
        1 agggaagaac acaagctatc attcgaaatc actttcttaa atatgataga gccgtccgat
       61 gtcgcgtcaa aattattact atggatatgt ttagtcctta ctatgactta gctagacaac
      121 ttttcccatg tgctaaaatc gtgttggatc gctttcacat tgtacaacat cttagccgtg
      181 ctatgagtcg tgtgcgtgtt caaatcatga atcaattgga tcgaaagtct catgaataca
      241 aggctatcaa gcgctactgg aagctcatac aacaggatag ccgtaaactg agtgataagc
      301 gattttatcg tcctactttt cgtatgcact taaccaataa agagatttta gacaagcttt
      361 tgagctattc acaagacttg aaacaccact atcagctcta tcaactcttg ctgtttcact
      421 ttcagaataa gaaaccggag aaatttttcg gactcattga ggacaatcta aagcaggttc
      481 atcctatttt tcagactgtc tttaaaacct tcctcaaaga taaagaaaag attgtcaacg
      541 cccttcaact acactattct aatgccaaac tggaagcgac caataatctc atcaaactta
      601 tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt caaaaaacgg atttttatcg
      661 ctctgaatat caaaaaagaa aggacaaaat ttgtcctttc tcgagcttag ctgacttcaa
      721 cccactacag ttgacaaaga gccaaaataa aactttcagg gttggtaggg gacttgagaa
      781 attagttgat tttctcgaca tagagttgtt ttgcaatgtc catattcact tgtaaatcct
      841 cgtcgttact gaggatagtg aaggtattgc tgaagccatc aaactgcttg acttggagct
      901 ggtcaccgat gtgaagtgag tgcttgtcca gataatggag aatgtcaaaa ctatcgtgca
      961 cccgagtcag gcggtaggcg ccagcttcct tgatatcagc tagtgggagg ttattgattt
     1021 caacgagtag ttctcccttg gcaggaatag ttcccccgtg ggggcaggtt ttagggaaac
     1081 ctagcagttt atctagtctt tccacgaaca ggtcagagac agtgtgttcc aagacctcag
     1141 cttcctcgtg aatctggtca cttgtatagt ctaaatgatg aactagaaaa acttcaatca
     1201 agcggtgctt acgatagagc tcagagacca gtttgagacc gaggtcagtc agtagatagc
     1261 cacattcctt gtcctttagg atgagatttt cacttttcat tcgtttgatc atttcagtta
     1321 cggcaggggg agagacttgc atgcgagccg caatttcctt gttggtaatc ttatgcaggt
     1381 ctatgccaat ttcataaata cattttagat agtcttcttt gtttggggtc attcgtttcc
     1441 tcttgtctaa tatctttatc tagtatatca aaaaaagcta gatttctcta gctgtgactt
     1501 tttatgttat acttattgca agagaaaaaa cgaggagatg gatatgacga aaatagctct
     1561 tctttcagat attcatggaa ataccaccgc cttggaggct gttttggcag atgctcggca
     1621 gataggagtg gatgaatact ggcttttggg agatattctc atgccaggga cagggcgtag
     1681 aaggattttg gacttgttgg atcaactacc gattacggct agagttttgg gaaactggga
     1741 agacagtctt tggcatggtg tccgtaagga attggatagt actcgcccca gtcaacgcta
     1801 tctcttgcgc cagtgccagt atgttttaga ggaaatttcc ctagaagaaa ttgaagtgct
     1861 ccacaatcaa cctcttcaaa ttcatcgtca gtttggggat ttgacggtgg gaattagcca
     1921 tcatctgcct gataagaact gggggcgaga gttgattcat actggcaaac aagaggagtt
     1981 tgaccgcttg gtgactcatc ctccctgtga tattgctgtt tatggacata ttcaccagca
     2041 gttgcttcgt tacgggactg gtgggcaatt gattgtcaat ccgggttcga ttgggcaacc
     2101 tttctttttg gacgctcagc tacggaagga cttgcgggcc cagtatatga ttttggagtt
     2161 tgatgacaaa ggcctggtag atatggactt ccgacgggta gactacgatg tggcagctga
     2221 attgcagctg gctaaagacc tgagacttcc ctattttgag gtttactatg aaagtctggt
     2281 caatgggatc caccatactc atcatcagga atttttgaga gaattggctc agaaagaggg
     2341 ctgcgatcgg gagttagacg actggttgaa aagtggtaac gattgacatt acaactaaaa
     2401 agggtataat aatagaaaaa gaagagtaga ggcagggtag cctcgtaaaa aggagaagag
     2461 gatgcaaatt ccaagtagat ttaccattgc gactcatatg ctgataatca ttgccctcga
     2521 agggaaggaa agcaaggtga ccagtgattt tctggctgct agtgtcgggg tcaatcctgt
     2581 cattatcaga aagatcttgt cccagttgaa gaaggcagag ctgatttcag tagcgcgtgg
     2641 aacgggcgga acagagattg tcaaggacct taaggatatt agtcttttag atgtttatca
     2701 ggcggtagaa tgtcttggta agacaggtca actcttcagt ttccatgaca atccgaatcc
     2761 aaattgccct gtaggagctc atattcatga tgttttggat caaaaattgg agagaattca
     2821 gttgactatg gaggcagaac ttggtcaaac cagtctagaa aaagtcgtgg ccgatgcaga
     2881 gagtcagatg aaggattaag agggataaat gccctctttt tttctttgga taattatgat
     2941 aaaatgtagc tattaaagga gtaagaaatg tatctcgtta taggaattgt attagccttt
     3001 atagtgtcat tttggaagta taatagaagt ttgtggaatc cagtattatt tttattatcc
     3061 ctcatttcaa gttattttta tctatcctac cttttttata aaaatggata cgagaatgtt
     3121 cagttagctt tttatgtatt tgcctttgta ttacttcctt tttttgcttt ttctaagtgg
     3181 catcttttta atctataatg gggtcatttt gttgaagcga gagggacgtt ccaagcccca
     3241 ttatctctca atgctctttg atttttattg actattagat tctatttaaa aagacttaga
     3301 atgcacatga aagtatgtat tctaggtctt ttgttttgta tacatttaat tttcaagagt
     3361 attatctatt tttccatacc tggtaacgag tatcaatcaa taactgggta aggcgaccca
     3421 aggtttcttt ttcttctgga gtgagggatt gagcttggac aaagagatga tgaatgtatt
     3481 caagcgcctt taaggaagcc aagtcattga tagagctaat accagcatca agaatagtgc
     3541 caaagaagag gtcgaagtag agctgaagtt cttcgtcagg gactgtggtc acccattctt
     3601 taaaggttgt gtctacttgc cggctatcac tgttggtctt atccagttga acgaagtgct
     3661 tatcctcaat ctgccaacta aaggtatcgt gctgggcgat gccacccagg gcagtactct
     3721 gaacgatgat ttggtgagca ggaatttcca gcatcatacc gataatggaa ccttgtggaa
     3781 tgaatatctt gcttctatcc attatccttt gataacccgc agtctgtgtc aattcttgat
     3841 ggagaccagg tgcatcaaat gtataaactg ctgtgatctg attttgcaaa ctttgctcaa
     3901 tttggctagc agcatagata gcgagatttc ctcccttgga atgcccagcc agaataacct
     3961 tttgcttagg atgatgggca aaaaagttct ttaaatagcg aagggcgtgc ttttgagcag
     4021 gaatttcctt catataggtc aggtggaaat cttccttcca gccaatgata ctgtcatctg
     4081 tcccacgaaa gacaatcaga taggtatcga ggctgacacg ataagtcata gccgcaaatt
     4141 gcttttgcag ttcagggtcg atgtcgttga taaaatggga gagtttgcaa tttttgaagc
     4201 gcttgtgttg agccaattca tctaataatt gaaggcgatt tttgctagta agcatggtgg
     4261 gctctcttgg aacctgaggt gctagatcta aaagacgttg aggaagtgtg gagaccagat
     4321 tatcaaagga gaggtaggtg atttctatta aggttagaat gtctaactca ttcaagggaa
     4381 ggtcgtaata agaatcatat gcgacatctt tcagatagtc aaaaatattg gccataagag
     4441 ctcctttctt tttatttatc ttagcaaatt tccctcaaat tagctagtag catagcctgt
     4501 ttgtactggc taaaaacagg ctatttcaaa ttcagtttca gaccatctag catggaaaaa
     4561 tctgttataa taatggaaaa ggagaagcgc atgcacaaga ttttattaat agaagatgat
     4621 caggtcattc gtcaacagat tgggaaaatg ctctctgaat ggggatttga agtggtcctg
     4681 gtagaagact ttatggaagt tttgagtcta tttgttcagt cggaacctca tctggtcctc
     4741 atggatattg gtttgccctt gtttaatggt tatcactggt gtcaggaaat ccgcaagatt
     4801 tccaaggtac ctatcatgtt tctttcttcg agagaccagg ctatggatat tgtcatggca
     4861 atcaatatgg gggcggatga ctttgtgacc aagccttttg accagcaggt tcttttagct
     4921 aaggttcagg gcttgttgcg tcgttcctat gagtttgggc gtgatgagag tttgctggaa
     4981 tatgctggtg ttatcctcaa taccaaatcc atggatttac attatcaagg gcaagtcttg
     5041 aatttgacca agaatgaatt ccagatttta cgcgtgttat ttgagcatgc aggcaacatc
     5101 gtagcacgtg acgacctgat gcgggaactt tggaacagtg actttttcat tgatgataat
     5161 accctctctg tcaatgtggc tcgtttgcgt aaaaagttgg aggagcaggg attggtagga
     5221 tttatcgaga ccaagaaagg aatagggtac ggattgaagc atgcttgatt ggaaacaatt
     5281 ttttctagcc tatctgcgct cccgtagtcg tctttttatc tatctgcttt ctttggcatt
     5341 tcttgtctta ctctttcagt ttttatttgc cagtctagga atttacttcc tctacttttt
     5401 cttcttgtgt tgctttgtaa ccatattatt tttcacttgg gacatattgg tggaaacgca
     5461 ggtctatcgc caggaacttc tctatggaga gagggaagcc aagtctcctt tggaaatagc
     5521 tttagcagaa aaattagaag cgcgtgagat ggaactctat cagcagaggt caaaagcaga
     5581 aagaaaactg acggatttgc tggattacta taccttgtgg gtccatcaga taaagacccc
     5641 cattgcagcc agtcaactct tagttgcaga agtggtcgac cgccaactga agcagcagct
     5701 agaacaggaa attttcaaaa tcgactccta taccaaccta gttttacagt acctgcgttt
     5761 agaaagtttc catgatgatt tggtcttaaa gcaggttcaa attgaggact tggtcaagga
     5821 aataattcgt aaatatgctc ttttctttat tcaaaaaggc ttaaatgtca atctacatga
     5881 ccttgataaa gaaatcgtga cggataaaaa gtggctgcta gtggttattg agcaaatcat
     5941 ctcaaacagt ctcaagtaca ccaaggaagg tggtctggag atttatatgg atgaccaaga
     6001 gctttgtatc aaagatacgg gaatcgggat aaaaaacagt gatgtcctcc gagtatttga
     6061 acgtggcttt tcaggataca atggccgttt gacccagcag tcctctggac ttggccttta
     6121 tctatctaag aaaatttctg aagaactggg gcaccagatt cgtatcgagt ctgaggtcgg
     6181 aaaaggaacg acagtgcgga ttcagtttgc tcaagtgaac ttagtccttg agtaaaaata
     6241 gatgagaaaa agcctccaga ttggaggctt ttatgctaga ctcttatttc tcaacgcgtg
     6301 atttgttggc gatatcagct aggatagctt gaacaaaatt atcaactgag acagtttgtg
     6361 tttctttttg gccgtagcga cgaacgttga ctgtttcgtc ttccatttct ttgtctccaa
     6421 caattaattg gtaaggaatc ttgctggttt gtgaagcacg gatcttgaac tgcatttttt
     6481 cattgcgctc atctacgtct gcacggacac cgcggtcacg gagtttcttg gctacttccc
     6541 aagcgtagtc cacgtgtttt tcgttagata ctgggataag ggttacttgg tgtggtgcca
     6601 gccatgttgg gaaggcaccc ttgtagttct caatcaagat agctgtgaag cgttccatag
     6661 ttgagataac cccacggtgg atcatgactg gacggtgatc ttcgccatca gctccgatgt
     6721 atttgaggtc gaagcgttct ggcaacaaga aatcaagttg gatagtagaa agggtttctt
     6781 cttttccaag ggcagtttta atctggatat ccaattttgg tccgtagaag gccgcttcac
     6841 cttcggcttc aaagtagtcc acgcccattt catcaagagc tgcacgaagc atggtttggg
     6901 cattttccca catctcatcg ttatcaaagt acttatgagt atcttgaggg tcacgaagag
     6961 agaggcggaa gcggtagtca gtcaagttga agtcttcata aacatcgata atcaactgaa
     7021 gggcacgttg gaattcttct tggatttgtt ctggagtaac gaataggtga ccgtcgttga
     7081 gtgacatttc acgtacacgt tgaaggccag tgagggcacc agatttttcg taacggtgca
     7141 tcataccgat ttcagcgata cggattggca attcacggta agagtgaaca tggtgtttga
     7201 aaacttggat gtggtgcgga cagttcattg gacgaaggac aaattcttcc ccgtcaccca
     7261 tgtccatggt tgggaacatg tcttcttggt aatgatccca gtggccagaa gtcttgtaaa
     7321 gctcaacaga agcaagtggt ggagtgtaga cgtgttggta gccagaagcc aactctttgt
     7381 ttacgatgta gcgttccaat tcacgacgga tagtcgcacc atttggcaac cagaatggca
     7441 aaccttgtcc cacttcttgt gaaatcataa agaggtcaag ctctttacca agtttacggt
     7501 ggtcacgttc cttagcttct tcacgcattt gaaggtagtt tttcaagtct ttcttttcaa
     7561 accaagctgt accgtagata cgttgcatca tagcgttgtc gctgtttcca cgccagtacg
     7621 caccagctac atggagaagg tggaagattt ggatacgacc tgttgatgga acgtgaggtc
     7681 cacggcagag gtctacatat tcaccctgac gatagatagt caaaccgcct tcgtcttctg
     7741 agtgttcttc aatcaattcc aacttgtaag ggtcattttt gaagatttca cgtgcctcgt
     7801 ctttagtcac ttcttcacga atagatggga agttttcttt gacgattttt tgcatttctt
     7861 cttcgatacg aggaaggtct tcgttagaga tttgaccagc tgtgttgtca gtatcgtagt
     7921 agaaaccatc ttcgatggct ggaccaactc ccaagtgaat gtctgggaaa agacgacgag
     7981 ctgcttgggc gaacaagtga gctgctgagt ggcgcaagat tggaagggca tcttcgtgat
     8041 caggtgtcac aatttcgatg cttccatctt cagtgatagc gcgagtagtg tcgatgagtt
     8101 tgccgttgaa tttaccagcc aaggcttttt tagctaggga attgctgata gattgggcaa
     8161 tttcaaaagt tgttacgcca gattcgaatt cacgaacagc gccatctggg aaagtaatgt
     8221 taatcatgat gttctccttt tttattattt catagtttct aagcattgat tttttatcat
     8281 cagactactt ccaagtccta acgttcaatt gcttttcttt atcagagagg aagttttcca
     8341 tttccgtcag aatgtctata tccaaacgtt gagaaagttc taatagccac cagatatttt
     8401 ctgaaagttt ttgttccagt gtgtagggtg tttcatcata gtagcgtcct tgctttgtca
     8461 tcaccagtcg ttggaaattt ccaatatcat tagataaagc caaggggtct tcttctaccg
     8521 tccacttgga atcatgatgc ttaacttcca gttcgtgata agcttgtcgg attgcccaac
     8581 tgcgctccac taattcttgc aactccataa ttagtatcct aataaaaaaa tgcgacatcc
     8641 tcgaaaggac gtcgcaacgt ggttccacct tcatttatgt acctcaaaag agagggacac
     8701 ctctgattgg ctctaacgtg gccaccgttt tatgttttca taaaaaactc aagagtagta
     8761 tcagtttaga cttcctatgc gtttccagca accacgcact ctctagtaga aagggactaa
     8821 gtgacttgtc tctatggatt attatagcat gattgtgaga tttttcaagg attttgtgaa
     8881 agttttttgt ttttcatttt ttgtagaata tgcttgatgc taataccaga atccatgccc
     8941 accagagcaa ctgtttcata gattagcaag taaattagta taaactcacc aaaggttatg
     9001 atagtaaagt aaaagagcag tccgttacca actaaccatc aaagtgagaa acggaaacgg
     9061 tagctataga gtaacgatac aatcaagatt accagaggtg taaagaagag aagattgttt
     9121 atatagagtt ccttgagtgt aagggggtca gaaacaacta gtaagaacaa ctcataaagt
     9181 ggccaataaa agaataatat gaccagatag taggggagat gggaatagaa tctacgcatg
     9241 ataatcacct cgttttaaaa aaacagacaa cttagatctc ttgctatgat tattatagca
     9301 tgtttaaaac aagaaagtaa atttgttggc aaagaagatt tttttttcac tagaatcatg
     9361 gttagttttc ttggtagaaa tattttgata ataattaagc aaaactattg acattgatta
     9421 ttaagagtgt cacaattgtt gtaactaaaa taattacaat aagagaggta taaaaaatga
     9481 aactagcagt tattgcagca aatggacaag cggggaaagc aattgttgaa gaagtagtca
     9541 aacgaggaca tgaagtaacg gctattgttc gttctgaaaa caaaagtcaa tctgagtcta
     9601 ttattaaaaa agatttattt gagttgacaa aagatgatct tactggattt gatgctgtta
     9661 tttcagcgtt tggtgcttat acaccagata cgctaccgct ccacagcaaa tcaatcgaac
     9721 tatttaatca gcttcttgct ggaacacaga cacgttttct agttgtaggg ggagcaggaa
     9781 gtctttatat agatgaaaca aaaacgacac gacttttaga tacgtctgat tttcctgaag
     9841 aatttaagtc actagcaaaa gctcaagcgg atgaattgga tcttcttcgt acaaaaaata
     9901 atctcaactg gacttttgtc agtcctgctg tagattttat tcccgatggt gaaaaaacag
     9961 gaaactacat tctagcagga gaaattttta ctactaatga aaaaggaata agccagatta
    10021 gttatgcaga ttatgctatt ggattggtag atgaacttga aaaaggtcat catataaaag
    10081 aacgtatctc tttacttgaa aaataataac tatgagagta ttaatgtttg acagaaaatt
    10141 taaatcttgg taaaataggg attgtcgtaa agacaaataa cttcttcttg gttacaggca
    10201 tgccaacctg tcactcggat gaagccaaat aaaaaggaga aacatcatgg caatctcaaa
    10261 agagaaaaaa aatgaaatca ttgcacaata tgcacgtcac gaaggtgata caggttcagt
    10321 agaggttcaa gttgctgtcc ttacttggga aatcaaccac cttaacgaac acatcaaaca
    10381 acacaaaaaa gaccacgcta cttaccgtgg attgatgaaa aaaatcggtc gccgtcgtaa
    10441 cttgcttgca tacttgcgta aaaacgacgt taaccgttac cgtgagttga tcaactctct
    10501 aggacttcgt cgctaattca agatacaaag gccgtcaaaa acacaaagca aaaataggaa
    10561 aattgacgaa gaaacttcag tttctaggag attttatctt ttttgccaag tgcttaggcc
    10621 gtgttcaatt gagcatatct tgataataaa gctactctaa attgggtagc tttgttgcgt
    10681 tgtcacctta cctcgctata ctcaagtata atcttcggtt acggttccta gcactgtaag
    10741 gtaaaataaa ctagcttttc tcccttcggg gagatttttt tgtttcactg aaagtttcgt
    10801 acgatcttca cctcgtcccc tagtctataa acgatttatc tagcaagaat catgatgcta
    10861 agggcgtcaa aaatccgtat gaaaataggg aaatgacaca gtgttcgatg agcacaagga
    10921 gtttcatctt tttcactagg attttagccc gggctaaaat cagctctctg acttcagagg
    10981 gcttttttat tgaggtttta tcggtaacaa ttttggagac tacaaaaacc tcaaatggta
    11041 tcagctaatt acaccataaa ggtgcgcagt tactcggctt ttcaagccga gtagctgtat
    11101 gcaagccccc tagaagagcc cactctttac aaagtaaagt atagtatgtt atactttaca
    11161 tggaagtagt cactgaattc cagttagaaa ttactttgta actacgtttt gaggaggagt
    11221 aaaatgcttt cctacgttcg acattaccca ctagcgatag ctaaattaat gtgtctgtgc
    11281 tctcctaaaa tctgctgatt tattactgac taatacagga ggtttttttt atgggacaga
    11341 caatcatatc tgctattggt gtttatattt ccaccagtat cgattattta attattttaa
    11401 ttattttatt tgcacagcta tcacagaata aacagaaatg gcatatttat gcggggcaat
    11461 atctaggcac aggcttactt gtaggggcga gtttagttgc tgcttatgtc gttaatttcg
    11521 tgcctgaaga atggatggtt ggattgcttg gtttaatccc tatctattta gggattcgct
    11581 ttgcaattgt tggagaagat gcggaagaag aagaggaaga aattattgaa agattagaac
    11641 aaagcaaggc aaatcaactg ttttggacag ttacattgct gacaattgcg tctggcggag
    11701 ataatttagg tatctatata ccttattttg cttcgttaga ttggtcacag accctcgtgg
    11761 ccttgcttgt gtttgtaatc ggcataatta tcttttgcga gattagtcgg gtgttatcct
    11821 ctattccgtt aatattcgag acaattgaaa aatacgagcg aatcattgtg cccttagtat
    11881 tcattctact tggactatac atcatgtatg aaaatggcac gatagagact tttctgatcg
    11941 tgtagatttt tttgtttcac taggatttta gcccgagctc aaatcagctc tctgattttc
    12001 agggggcttt ttcattgagt ataaatggac aatcttaaag ttactctatt gtagagtggc
    12061 ttttgatttg attaggagat tcttataaat atagtaaaat gaaataagaa taggacaaat
    12121 cgatcaggac agtcaaatcg atttctaaaa atgttttaga agtagaggtg tactattcta
    12181 gttccaatct actatataaa tgaaaacctt agctgtttgg ccaaggttca tttcttctat
    12241 gcaagttctt ctcttttctt atctgggttc cagatgaagc ttgcgataaa gctaaagatg
    12301 atggttagga tagcgaggat aatagcaagg atgagggcag ggatgcggat aaaccaccag
    12361 agtggatttg gttttttatc attgtgccat tgtttcgttt cttcgtccag acgttggaat
    12421 tctgtttgaa tacgattggc gactgtttca atgccttcat cattcatttt cttgatatct
    12481 gagatatcga ttggatttcc aaagttcata tcgacacgtt cacggctaat caagcccttc
    12541 aaagtcatgg gaccggtgta ggtaaccggc atgatacgga ccttggccat tttggcaatc
    12601 agtgctgcgc cccccttgac atcgtttgag tggcggctac cacttggaaa catgatgaga
    12661 gagcggtcac tttttttgag aacgttgata ggatatttga tggctgaggc gctgggattt
    12721 tcacggtcga tgggaaaggc gccacacata cgaatccacc aaccaaagat acggttggta
    12781 aagagttctt tttttgccat aaagatgaac tgttttggct tggtcgcaaa ggccatataa
    12841 acaggatccc accaggtacg gtgaggcgca actaaaatat aattttcatc ttgattagga
    12901 attttatcag tattatgata gtgagcattg ccattgatgg accataagag caatacaact
    12961 aatccacgca aataagtata aaacatgcga tctccttcaa ttgttttctt gttattatta
    13021 taccttatca aaggagggct ggcaaacttt tcccttgact agatgcatat ttgggatgaa
    13081 attagaattc tgttaaaaaa aatgatataa tagaatttat ggataaaaat aagattatgg
    13141 gattaaccca aagagaagtc aaggaaagac aggctgaggg tttggtcaat gactttaccg
    13201 catcagccag taccagcact tggcaaatcg ttaaacgaaa tgtctttacc ctttttaacg
    13261 ctttgaactt tgccattgct ttggcccttg cctttgtgca ggcttggagc aatctggtct
    13321 tctttgctgt tatctgcttt aacgcttttt ctgggattgt gaccgagcta cgagccaaac
    13381 acatggtgga caagctcaat ctcatgacca aggaaaaggt caaaaccatc cgtgatggtc
    13441 aggaagttgc tcttaatcct gaagaattag tgctaggaga tgtcattcgt ttgtctgcag
    13501 gagagcagat tcctagtgat gccttggttt tggaaggctt tgcggaagtc aatgaagcca
    13561 tgttaacggg agaaagtgat ttggtgcaaa aggaagttga cggcttactt ttgtcaggaa
    13621 gtttcctagc cagtgggtca gttttatctc aagttcacca tgtcggtgca gacaactatg
    13681 ctgccaaact catgcttgag gctaagaccg ttaaacccat caactcccgt atcatgaaat
    13741 cgctggacaa gttggctggt tttactggga agattatcat tccctttggt ctggctctct
    13801 tgctggaagc cttgctttta aaaggcctgc ctctcaagtc atctgttgta aactcgtcga
    13861 cagctctttt gggaatgttg cctaagggaa ttgccctttt gaccattact tcgctcttga
    13921 ctgcagtgat taagctaggt ttgaaaaagg tcttggtgca ggagatgtac tctgttgaga
    13981 ccttggcgcg cgtggatatg ctctgtctgg acaagacggg taccatcacc caaggaaaga
    14041 tgcaggtgga ggctgttctt ccactgacgg aaacttatgg tgaagaggct gttgccagca
    14101 tcctgaccag ctatatggcc catagtgagg ataaaaatcc aacagctcaa gccattcgcc
    14161 agcgttttgt gggagatgtt gcttatccta tgatttccaa tcttcccttc tcgagcgacc
    14221 gcaagtgggg ggctatggag ttagaaggtt tgggaatagt tttcttaggg gcacctgaga
    14281 tgttgctgga ttctgaagtc ccagaagcca gggaggcctt ggagagagga tcacgtgtct
    14341 tggtcttagc tctcagtcag gagaaattag accatcacaa accacagaaa ccatctgata
    14401 ttcaggctct agccttgctg gaaatcttgg accccattcg agagggagca gcagagacgc
    14461 tggactatct ccgttctcag gaggtgggac tcaagattat ctctggtgac aatccagtta
    14521 cggtgtccag cattgcccag aaggctggtt ttgcggacta tcacaactat gtagattgct
    14581 caaaaatcac cgatgaggaa ttgatggcca tggcggagga gacagcgatt ttcggacgtg
    14641 tttcccctca tcaaaagaaa ctcatcatcc aaacgttgaa aaaagcgggt catacaacgg
    14701 ctatgacagg ggacggggtt aatgatatct tggcccttcg tgaggcggat tgttctatcg
    14761 tgatggcgga gggggatcca gcaacccgtc agattgccaa tctggttctc ttaaactcag
    14821 actttaatga tgttcctgag attctcttcg agggtcgtcg cgtggtcaat aacattgccc
    14881 acatcgcccc gattttcttg ataaagacca tctattcctt cctgttagca gtcatctgta
    14941 ttgccagtgc tttactaggt cggtcagagt ggattttgat tttccccttc attccgatcc
    15001 agattaccat gattgaccag tttgtggaag gtttcccacc attcgttttg acttttgagc
    15061 gaaatatcaa acctgtcgag ccaaacttcc tcataagatc catgcttcgt gccctaccaa
    15121 gtgccctcat ggtcgtgttt agcgttcttt ttgtgaaaat ctttggaacg agtcaaggtt
    15181 ggtctgagtt agaaatctca actctcctct attatctctt ggggtcaatt ggtttcttat
    15241 ccgtatttag agcctgcatg ccattcactc tatggcgagt cctcttgatt gtctggtcag
    15301 taggaggctt cctagccaca gctctcttcc caagaatcca aaaactgctt gaaatttcaa
    15361 ccttgacaga acaaacgttg cctgtttatg gtgtcatgat gttggtcttt acagtgattt
    15421 tcatcctgac tagtcgctat caagctagaa aataaagaaa gactgcaatc tgtggattgc
    15481 agttttttta ggtacaagat tgccagccga aatgtggtat aataagaggt aatagagttt
    15541 tggaaagtga gagaagatga tttcaaagag attagaattg gtagcttcct ttgtgtcaca
    15601 gggggctatt ttactagatg tgggaagtga ccatgcttat ctgcctatcg agttggttga
    15661 gagaggccaa atcaaaagcg ctattgcagg tgaggtggtg gaaggtccct atcagtctgc
    15721 ggttaaaaat gttgaggctc acggcctaaa ggagaaaatc caagtccgtt tagccaatgg
    15781 cttggcagct tttgaagaga ctgaccaagt gtctgtcatt accattgctg gcatgggtgg
    15841 tcgtttgatt gctaggattt tagaagaagg tttggggaag ttagctaatg tagaacgttt
    15901 gatcctccag cccaataatc gtgaagacga cttgcgtatc tggctacagg atcatggatt
    15961 ccagattgta gcagaaagca tcttagaaga agctggaaag ttttatgaga ttttggtggt
    16021 ggaagcagga caaatgaagc tatcagccag tgatgttcgc tttggtccct tcttgtccaa
    16081 agaagtcagt ccagtatttg tccaaaaatg gcaaaaagaa gctgagaagc tagagttcgc
    16141 cctcggacaa atcccagaaa aaaatctgga agaacgtcaa gttctagtag ataagattca
    16201 agctatcaag gaggtgctcc atgttagcaa gtgaagtgat ccaagcttat gaagcctttt
    16261 gccctcagga attttctatg gagggagaca gctgtggtct gcaaattggc actttagaca
    16321 agggtatcca aagggtcatg gtggctctcg atattcgtga agagacggtg gctgaagcca
    16381 ttgaaaaggg tgtagacttg attatcgtca agcacgcgcc tatctttcgt cctatcaagg
    16441 acttgcttgc cagccgtccg caaaatcaga tttacatcga cctgattaag catgacatcg
    16501 cagtttatgt cagccatacc aatattgata tcgttgaaaa tgggctcaat gactggtttt
    16561 gtcagatgct aggaatcgag gagacgactt atctgcagga aacaggtcca gaacgtggaa
    16621 ttggacgtat tgggaatatt cagcctcaga cattttggga attggcccaa caggtcaagc
    16681 aagtctttga cctagatagc cttcgaatgg tgcattatca agaggatgat ttgcagaagc
    16741 ctatttcaag agtagcaatt tgtggtggaa gcgggcagtc tttctataag gatgctttgg
    16801 caaagggggc agatgtctat atcactggtg atatctacta ccatactgct caggatatgc
    16861 tgtctgatgg cttgttagca ttggatccag gtcactatat cgaagtgctt tttgtggaaa
    16921 aaatcgcaac actccttact caatggaaag agaaaaaagg ctgggaacta gagattttac
    16981 ctagtcaagc atcgaccaat cctttccgtc atatctagtt agaaagtgaa gacaatgaaa
    17041 aaagttgcca ttattggagc aggaattgtg ggagcaacag ctgcctacta cctctcgaga
    17101 gaaagtgacc tagaggtgac cgtttttgac catgagcaag gtcaagccac caaggccgca
    17161 gcaggaatta tcagtccttg gttttccaaa cgccgtaata aagcctggta caagatggcg
    17221 cgcttggggg ctgattttta tgtggattta ttagctgatt tagagaaatc aggacaagaa
    17281 atcgactttt accagcgttc gggagtcttt ctcttgaaaa aggatgaatc caatttggaa
    17341 gaactttatc aactggccct ccagcgcaga gaagaatctc ccttgatagg gcaattagcc
    17401 attctgaacc aagcctcagc taatgaatta ttccctggtt tgcagggatt tgaccgcctg
    17461 ctctatgctt ctggtggagc gagagtagat ggtcacctct tagtaactcg tttgctggaa
    17521 gccagtcagg tcaagttggt caaagaaaaa gtgactctga cactcttatc atcaggctac
    17581 cagattggcg aagaggtgtt tgatcaggtt attttggcaa cgggagcttg gttgggggac
    17641 atgttagagc ctttaggtta tgaagtggat gtccgtcctc aaaaaggaca actacgagat
    17701 tatcagcttg cccaagacat ggaagattac cctgttgtca tgccagaagg ggagtgggat
    17761 ttgattccct ttgcaggtgg gaaattatcc ttgggcgcta cccatgaaaa tgacatggga
    17821 tttgatttga cggtagatga acccttgctc caacaaatgg aggaggcggc cttgcctcat
    17881 tacccagttt tagctagagt gacttcaagg gctgagcgtg tgggaatccg tgcctatacc
    17941 agtgatttct ctcccttctt tgggcaggtg ccagaattgg caggtgtcta tgcagccagt
    18001 ggactaggtt catcaggcct cacaactggt cctatcattg gttaccatct agcccaactg
    18061 atccaagaca aggagttgac cttggaccct gaaaattacc caattgaaaa ctatgtcaaa
    18121 cgagtaaaaa gcgaataata ctcaatgaaa atcaaagaac aaactaggaa actagccgcg
    18181 ggttactcaa agcactgctt tgaggttgtg gatagaactg acgaagtcag ctcaaaacac
    18241 tgttttgagg ttgtggatag aactgacgaa gtcagtaacc atacccatgg caaggcgacg
    18301 ttgacgtggt ttgaattgga ttttcgaaga gtataagcat tttactgaaa ttttatcagg
    18361 tagtttagga tggcaaatga cattccctgt caaaaatggt aaaataagaa aaaatgatct
    18421 gagaatcgag gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc cggttttatt
    18481 ggaacccaca ctgttattga attgatccaa gcaggccatc aagttgttgt ggtggataat
    18541 cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg agttgagatt
    18601 cctttctatg aggcagatat tcgtgacaca gataccctca gagatatttt caagcaggaa
    18661 gaaccgactg gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc aacacgtatc
    18721 cctcttgcct actatgacaa caatatcgct ggaactgtca gccttttgaa agccatggaa
    18781 gaaaacaact gtaaaaacat catcttcagt tcttctgcga cagtttacgg agatccgcac
    18841 acagtgccca tcttggaaga tttcccactt tcagtgacaa acccatacgg tcgtaccaag
    18901 ctcatgctag aggaaatttt gaccgatatt tacaaagcag actcagaatg gaatgttgtc
    18961 ttgcttcgtt actttaaccc aatcggagcc catgagagtg gtgatttggg agaaaatcca
    19021 aacggtattc caaacaatct cttgccatat gtgactcaag tagccgttgg aaaattagag
    19081 caagtgcaag tgtttggaga cgattacgat acggaagatg gaacaggtgt tcgtgactat
    19141 atccacgttg tcgatttggc taagggtcac gttgcagctt tgaaaaaaat ccaaaaaggt
    19201 tcaggactaa acgtttataa ccttggaact ggtaaaggtt actcagttct tgaaattatc
    19261 caaaacatgg aaaaagcggt gggacgtcct attccttacc gcatcgtaga acgtcgccca
    19321 ggtgatatcg ctgcctgcta ctcagaccca gcaaaagcta aagcagaact cggttgggaa
    19381 gcagaactcg acatcaccca aatgtgtgaa gacgcatggc gttggcaaag caagcatcca
    19441 aatggatttg aagactaaga tgatgatttc aatcatcgtc ccttgtttaa acgaagagga
    19501 agtacttcct cttttttatc aggctctgga agctttactt ccagatttgg aaacagaaat
    19561 cgagtatgtc tttgtcgatg atggatcaag tgatgggacc ttggaactct taaaggccta
    19621 tcgggagcaa aatccggcag tccattatat ttctttctct cgaaattttg gcaaagaagc
    19681 agccctttat gcaggcttgc aatatgcgac aggagatttg gtggtggtga tggatgcaga
    19741 cctccaagat cctcctagta tgttgtttga gatgaaaaat gtactagaca aaaatgtaga
    19801 cttggactgc gttgggacac ggagaactag tcgggaggga gaacccttct ttcgcagttt
    19861 ctgtgctgtt ctcttttatc gcctcatgca aaaaatcagc ccagtagctc tgccgtcggg
    19921 tgtccgtgat tttcgtatga tgagaaggtc tgtagtcgat gccattttaa gcttgaccga
    19981 gtccaatcgt ttttcaaagg ggctcttcgc ctgggtgggt ttcaaaacct actatttgga
    20041 ctatccaaat gtcgaaaggc aggctggcaa gacaagttgg agttttaggc aactcttttt
    20101 ctactccatt gaagggatta ttaatttttc agattttccc ttgattatag cctttgtagc
    20161 tggtctccta tcttgttttc tttctctgct gatgaccttt tttgttgtgg ttcggaccct
    20221 cattttgggc aatccgacat ctggttggac ctctctgatg gctgttattc tctttcttgg
    20281 aggcattcaa ctcttgacca ttgggattct cggcaagtat atcagtaaga tttatttaga
    20341 gactaaaaaa agaccacttt atcttatcaa agaaaaaagt gaccttcctg attttacaga
    20401 aaaaaataaa gtgaaaagac tataatttta catggaaatg tgctaaacta gagggagtgg
    20461 attatcgccc ttgatttagg cttcctggtt tccaatcagg gcgcgagttg ggcaattttt
    20521 aacagcctct aagacatcct gactaggaga aatttctttt tccagttggt cagggtcatc
    20581 gtaaaaacgc acgattccat tatcgtggta atcaaataaa tcagaataag tttggcaaag
    20641 cccacaggcg atacatcgtt caggtataag tgtgattttc atgttcatat ttatattgta
    20701 ataagaaaag taaaaaaaaa caaggagtaa ggtatggcaa aagaaccgtg gcaagaagat
    20761 atctatgatc aagaagaatc aagagcagag cgtcggcatc gaaaccacgg aggggctgat
    20821 aggatggcta atcgtatttt gacgatccta gctagtattt tctttgtaat tgtggtggtg
    20881 atggtcatcg ttctcatcta tctatcatcg gggggaagta atcgcacagc agccttaaaa
    20941 gactttcatg attctgatgc aagtgtagta caaatctcat cttcaagcag ttctcatcct
    21001 gagcagagtt cagagccaga atctacttct agtagttcag aagaagctgc taatcctgaa
    21061 ggaacgatta aagttctcgc aggagaaggg gaagcagcta ttgccgctcg tgcaggaatc
    21121 tccattgctc agttagaggc cttgaatcct gggcacatgg ctacaggatc ttggtttgct
    21181 aatccaggtg atgttataaa aataaaatag gagccgatat gaaaacaatt caaattgcta
    21241 ttgatggtcc tgcttccagc ggtaagagta cggtcgcaaa gattattgct aaggattttg
    21301 gattcaccta ccttgataca ggagctatgt atcgtgcagc gacctatatg gctcttaaga
    21361 accaattagg agttgaagaa gtcgaagccc ttctagcctt gttggaccag catccaatca
    21421 gctttggacg ttcagaaact ggagaccagc ttgtttttgt aggagatgtg gatattaccc
    21481 atcctatccg tgaaaatgaa gtgaccaatc atgtttctgc tattgcagca attcctcaag
    21541 tgcgtgagaa actggtttct ctccaacaag aaattgccca gcaaggcggg attgtcatgg
    21601 atggtcgcga tattggaact gttgtattgc cacaagcaga attgaaaatt ttcctagtag
    21661 cttctgttga tgagagagca gagcgtcgtt acaaggaaaa tattgccaag ggaattgaaa
    21721 cagaccttga aaccctaaaa aaggaaattg ctgcgcgtga ctacaaggat agtcatcgtg
    21781 agacttctcc tctcaaacaa gcagaggatg ctgtctacct tgatacaact ggtttgaaca
    21841 ttcaagaagt agttgaaaaa atcaaagcag aagctgaaaa aagaatgtaa atgtagaaaa
    21901 gccgatagat tgatatcggt ttttttctaa atttgtcaaa atgctgattt tagggtataa
    21961 tagttgctgt tcgagaaatt ttgattttca aagaatagtg aatgatgata aggagaaacc
    22021 atgaacaatt taccaaattg tccaaaatgt aactcagagt atgtctacga agacggtgcc
    22081 ctactggttt gcccagagtg tgctcatgag tggaatcctg ctgaagttgc agaagtagaa
    22141 gaaggtcttg tcgctatcga tgccaacgga aataaattga ctgatggtga tacagtaact
    22201 ctcatcaagg acttgaaagt aaaaggtgcg ccaaaagatt tgaaacaagg gacgcgcgtg
    22261 aaaaatatcc gcatcgtaga aggcgatcac aatatcgact gtaagattga tggttttggt
    22321 gccatgaaac ttaaatcaga gtttgtgagg aagatttaag catgtcaaaa cactataaac
    22381 ttgtatttta tagccgtatc ttcttgtttc tagcggcttt tacgggagtt tatcttgaaa
    22441 ttactaggca tggtggtttt gggatgcttc tctattacac ggttctgtcc aacctcttgg
    22501 taactatttt taccctttat cttctaaagg ttatgagccg tgtaggtgaa aattggcaaa
    22561 ggccaagtct cttgcgctta aaaggtgggg tcaccatgag tatcatgatt acctgtgtga
    22621 tttaccattt cctcttggcg cccattgcga ctaatttcta taccctagaa aatttccttt
    22681 gccactatat cgttcccatc tggtttttag cggataccct cttttttgac aaacagggtc
    22741 aatacaagat ttgggatcca gcagtgtgga ccattttacc ctttctgtat atgatgtttg
    22801 ctctttttaa tggcttggtt ctaaaactca atattccaaa tgccaaggat aatcctttcc
    22861 cttacttctt tttgaatgtg aacaagggtt ggaatgttgt gtttaagtgg tgtctgatta
    22921 tctttgttgc ctatatggta gcaggattta ttttctactt tatcaagcaa atcaagagaa
    22981 agtcatccta atactcttcg aaaatctctt caaaccacgt cagtttccat ctgcaacctc
    23041 aaaacactgt tttgaacaac ctgcggctag cttcctagtt tgctctttga ttttcattga
    23101 gtataagata caagggattc attttcgaat ttaagaagga gtctacaaac caatgaggct
    23161 tcttcttttc ttgcttattt gataactatc aaaaaagtag aagattaaga gaaatataga
    23221 aagagatttc atgaaacaat ttttagaacg ggccagcatt ttggctctct ccctcgtttt
    23281 gattacctcc ttttccattt cgagtgccct accagccatg tttgactatt atcagggtta
    23341 ttctaaggaa caaattgagc tcttggtgag cttgccttcc tttggaatca tgatgatgtt
    23401 actgctaaat ggtttcttag aaaaaatatt tcctgagcgc ttacagatta gtttgggctt
    23461 gctgatttta tcattgagcg gtacagctcc cttctggtac caagcctatc cctttgtctt
    23521 tggaacacgg cttctctttg gtttgggtct tgggatgatc aatgccaagg ccatttctat
    23581 tatcagtgaa cgctaccaag gaaaaaggcg aattcagatg ttagggctac gcgcttctgc
    23641 agaggtcgtt ggagcttctc tcattacctt ggccgtcggt cagttgttgg cctttggttg
    23701 gacagctatc tttctagcct atagtgctgg atttttggtg ctgccccttt atctgctctt
    23761 tgtcccttat ggaaaatcaa agaaagaagt caagaaaaga gcgaaggaag caagtcgttt
    23821 aactcgagaa atgaaaggct tgatttttac cttagctatc gaagcggcag ttgtagtttg
    23881 taccaataca gctattacca tccgtattcc aagtttgatg gtggaaagag gattggggga
    23941 tgcccagtta tctagttttg ttcttagtat catgcagttg atcgggattg tggctggggt
    24001 gagtttttct ttcttgattt ctatctttaa agagaaactg ctcctctggt ctggtattac
    24061 ctttggcttg gggcaaatcg tgattgcctt gtcttcatcc ttgtgggtgg tagtagcagg
    24121 aagtgttctg gctggatttg cctatagtgt agtcttgacg acggtctttc aacttgtctc
    24181 tgaacgaatt ccagctaaac tcctcaatca agcaacttca tttgctgtat taggctgtag
    24241 tttcggagcc tttacgaccc cattcgttct aggtgcaatt ggcttactaa ctcacaatgg
    24301 gatgttggtc tttagtatct taggaggttg gttgattgta atctctatct ttgtcatgta
    24361 cctacttcag aagagagctt aggattgatt cctaagtttt tcttttgata ctcaatgaaa
    24421 atcaaagagc aaactatagt tgattgagtt tggaatagta cgctgtagct gctaaaatat
    24481 ttctagaaat taatttgact ttcctaatag agttgttcat atcttatttc aatctactat
    24541 agaaagctag ccgcaggttg ctcaaaatag tgtgttgagg ttgcagatgg aaactgacgt
    24601 ggtttgaaga gattttcgaa gagtatgaga tttttatacc catacaatat ttgttttagg
    24661 tccttgttta cagactaatt tcttgataaa atagaggcta tgacgagata taaagcaact
    24721 atttcctatg atggttatgc ctttgctggc tttcagcgcc agcctcatgc gcgtagcgtt
    24781 caggaagaaa ttgaaaaaac cttgaccaga ttaaataaag ggcaagccat tactgttcac
    24841 ggtgctggta ggacagatag tggggttcat gccctgggac aggtgattca ttttgacctg
    24901 ccttatcaga tggatgagga gaaactccgt tttgccttgg atacccagtc tcctgaagat
    24961 attgatgtga tttcgattga gcttgtggca gatgattttc attgccgtta tgccaagcat
    25021 agcaagacct atgagtttac tgtggataga ggacgtccca aaaatccgat gcgccgtcac
    25081 tatgccaccc actttcccta cccactcgat gtggaacgaa tgcagattgc aatcaaaaag
    25141 ctagagggaa cccatgattt taccggtttt acagcctctg ggactagtgt agaggataag
    25201 gttcgcacca tcacagaagc tagtttaata gtcgatgaga caggacaatt tttgaccttt
    25261 accttttcag gaaatggttt cttgtataaa cagattcgca atatggtggg gactcttctc
    25321 aaaatcggta acaaccgcat gccagtagag cagattgatc tcatcttgga gaagaaggac
    25381 aggcaacttg caggtcccac tgcagcacca aatggtttgt atttaaagga gattcgttat
    25441 gaagaataat cgtattttag cactttctgg aaatgatatt tttagtggtg gtggactgtc
    25501 agctgatttg gctacctata ccttgaacgg cttgcatggg tttgtagcag tgacttgttt
    25561 gacagccttg acagaaaaag gatttgaagt ctttccaact gatgatacca tttttcaaca
    25621 tgaattagat agcttgcgtg atgtggaatt tgggggaatt aagattggtc ttctccctac
    25681 tgtcagcgtg gctgagaagg ccttggactt tatcaaacaa cgcccaggag tacctgtggt
    25741 gttggatcct gtcttggtct gcaaggaaac gcatgatgta gctgtcagtg agctctgcca
    25801 agagttgatt cgctttttcc cttatgtcag tgtgattacg cctaatctcc cagaagcaga
    25861 attattatcc ggtcaggaaa ttaaaacctt ggaagacatg aaaactgcag cgcagaaatt
    25921 gcatgattta ggagcgccag cagtcattat caagggaggc aatcgtctta gtcaggacaa
    25981 ggctgtggat gtcttttatg atggacagac ctttactatc ctagaaaatc cagttatcca
    26041 aggccaaaat gctggtgcag gttgtacctt tgcctctagc attgccagtc acctggttaa
    26101 aggtgataaa cttttgccag cagtagaaag ctctaaggct ttcgtttatc gtgctattgc
    26161 acaagcagat cagtatggag taagacaata tgaagcaaac aaaaacaact aaaatcgccc
    26221 ttgtatccct attaaccgcc ctttctgtgg ttctaggtta tttcttaaaa atcccaacac
    26281 ctacaggaat tctaactctt ttagatgctg gtgtcttctt tgcggccttt tactttggta
    26341 gtcgtgaagg agcggtagtc ggaggactag caagtttctt gattgacctc ttatcaggct
    26401 accctcagtg gatgttcttt agcttggtca accatggctt gcagggattt ttcgcaggat
    26461 ttaaaggaaa aagtcagtgg ttaggcctta ttttagcaac tattgccatg gtaggaggct
    26521 acgccttggg ttctgctttg atgaatggct gggcagcagc cctcccagaa attctaccga
    26581 attttatgca aaatatggta gggatgattg taggatttat tcttagtcaa agtatcaaga
    26641 agattaagta atactcttcg aaaatctctt caaaccacgt cagcttccat ctgcaacctc
    26701 aaaacactgt tttgagcagc ctgaggctag ctttctagtt tgcactttga ttttcattga
    26761 gtataaagag gttgagtaaa aagtatttca aataagaaaa acgcatagta tcaggtgttg
    26821 aatgaactgc
//
LOCUS       DAVHEW010000015        25403 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVHEW010000015 DAVHEW010000000
VERSION     DAVHEW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.015"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(186..1748)
                     /gene="guaA"
                     /locus_tag="VVL53_000641"
     CDS             complement(186..1748)
                     /gene="guaA"
                     /locus_tag="VVL53_000641"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU4182849.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVH
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1890..2588
                     /locus_tag="VVL53_000642"
     CDS             1890..2588
                     /locus_tag="VVL53_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4182850.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2631..3527)
                     /locus_tag="VVL53_000643"
     CDS             complement(2631..3527)
                     /locus_tag="VVL53_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU4182851.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3536..4471)
                     /locus_tag="VVL53_000644"
     CDS             complement(3536..4471)
                     /locus_tag="VVL53_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU4182852.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4468..5193)
                     /locus_tag="VVL53_000645"
     CDS             complement(4468..5193)
                     /locus_tag="VVL53_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU4182853.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5277..5912)
                     /locus_tag="VVL53_000646"
     CDS             complement(5277..5912)
                     /locus_tag="VVL53_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU4182854.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(5914..6741)
                     /locus_tag="VVL53_000647"
     CDS             complement(5914..6741)
                     /locus_tag="VVL53_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4182855.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6763..7689)
                     /locus_tag="VVL53_000648"
                     /pseudo
     CDS             complement(6763..7689)
                     /locus_tag="VVL53_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8350..8961
                     /locus_tag="VVL53_000649"
     CDS             8350..8961
                     /locus_tag="VVL53_000649"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4182856.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9006..9734
                     /gene="rlmB"
                     /locus_tag="VVL53_000650"
     CDS             9006..9734
                     /gene="rlmB"
                     /locus_tag="VVL53_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU4182857.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="VVL53_000651"
     CDS             complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="VVL53_000651"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU4182858.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10750..10974)
                     /locus_tag="VVL53_000652"
     CDS             complement(10750..10974)
                     /locus_tag="VVL53_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU4182859.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11052..11795)
                     /locus_tag="VVL53_000653"
     CDS             complement(11052..11795)
                     /locus_tag="VVL53_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4182860.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11795..12595)
                     /locus_tag="VVL53_000654"
     CDS             complement(11795..12595)
                     /locus_tag="VVL53_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4182861.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12753..13109)
                     /locus_tag="VVL53_000655"
     CDS             complement(12753..13109)
                     /locus_tag="VVL53_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU4182862.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13103..13633)
                     /locus_tag="VVL53_000656"
     CDS             complement(13103..13633)
                     /locus_tag="VVL53_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU4182863.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(13633..14127)
                     /locus_tag="VVL53_000657"
     CDS             complement(13633..14127)
                     /locus_tag="VVL53_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4182864.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKMPVDGERLAYQ
                     KLKK"
     gene            14262..14708
                     /locus_tag="VVL53_000658"
     CDS             14262..14708
                     /locus_tag="VVL53_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU4182865.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14705..15352
                     /locus_tag="VVL53_000659"
     CDS             14705..15352
                     /locus_tag="VVL53_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU4182866.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="VVL53_000660"
     CDS             complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="VVL53_000660"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU4182867.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="VVL53_000661"
     CDS             complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="VVL53_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU4182868.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16982..18361)
                     /locus_tag="VVL53_000662"
     CDS             complement(16982..18361)
                     /locus_tag="VVL53_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4182869.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18655..19578
                     /locus_tag="VVL53_000663"
     CDS             18655..19578
                     /locus_tag="VVL53_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU4182870.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19638..20243
                     /locus_tag="VVL53_000664"
     CDS             19638..20243
                     /locus_tag="VVL53_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4182871.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20262..21506
                     /locus_tag="VVL53_000665"
     CDS             20262..21506
                     /locus_tag="VVL53_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4182872.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21729..21986)
                     /locus_tag="VVL53_000666"
     CDS             complement(21729..21986)
                     /locus_tag="VVL53_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU4182873.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22028..24064)
                     /locus_tag="VVL53_000667"
     CDS             complement(22028..24064)
                     /locus_tag="VVL53_000667"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU4182874.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="VVL53_000668"
     CDS             complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="VVL53_000668"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU4182875.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQDCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
BASE COUNT         7485 a         5729 c         4641 g         7548 t
ORIGIN      
        1 attctcatta tatatatcaa aagaccgcag cttgaaaact acggtctaat tttttgtcca
       61 aatgtaaacc acttggaact attattcaat ttattctttt tagcaaatgt tcccgtttta
      121 atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt ttaatttttt
      181 tgcgattatt cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg
      241 ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga
      301 attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg
      361 tagtcatacg tacgaccgtc acccataaca ccgactgaat gaacgcctgt gttaacagtg
      421 aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg
      481 tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca
      541 agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta
      601 ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc
      661 atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta
      721 ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc
      781 ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt acgtttttgt
      841 tcagggtcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg
      901 atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga
      961 agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga
     1021 accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg
     1081 acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct
     1141 ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga
     1201 cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg
     1261 tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca
     1321 tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt
     1381 gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc aggaacaact
     1441 tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa aattggaatt
     1501 ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga
     1561 ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta
     1621 tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg
     1681 tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg
     1741 ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa
     1801 gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat
     1861 agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatataaaa atccatgatc
     1921 agataaaaaa ggatattgac gagtaccgtt gggctattgg tgagaggctt cccagtgaaa
     1981 gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat
     2041 tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc
     2101 gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta
     2161 aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg
     2221 ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg
     2281 accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta
     2341 aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc tatcgtatcg
     2401 gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg
     2461 aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta gaagatggta
     2521 ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa
     2581 ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct
     2641 aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga
     2701 aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaat cgccgatatt
     2761 ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg
     2821 aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa
     2881 tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc gaccaaacct
     2941 gctatgacca ttcccacacc aatgaccaag ctgctccccc actttttaat caaggctatg
     3001 ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa cagaccagca
     3061 ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg
     3121 gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta
     3181 taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc
     3241 tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata
     3301 agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta
     3361 gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt agtcaagacc
     3421 agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa cccccaagca
     3481 ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact
     3541 cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt
     3601 ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca
     3661 tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta
     3721 aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat
     3781 ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcac
     3841 tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac
     3901 ctctaactgt tggaacagca agctcaactg gcccaaactt agtttccgtc attccccaag
     3961 gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa
     4021 taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt
     4081 tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa
     4141 aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac
     4201 tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga
     4261 aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc
     4321 ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac tctgtccatt
     4381 gattgtcctt aaaatacgca aaagaggctc cgggataaat ccctgcctcg atttgttctt
     4441 ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt
     4501 tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa
     4561 cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc
     4621 ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga
     4681 tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat
     4741 agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc
     4801 tcctacttct actagacttg ctatgtcatc ttccgcatga atcaaaatca aatcatcttc
     4861 tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc
     4921 tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc
     4981 tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag
     5041 cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt
     5101 cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt
     5161 aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa
     5221 aggattttag aattttttac aagatattat gctcaaattt aacattattt agctatttag
     5281 taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact gaggtgcagt
     5341 ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact
     5401 ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg attccagttc
     5461 tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctct ctcattgaca
     5521 ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg
     5581 atagcttcga gattattgag agcctcattc acaggaacat cctttccgat atcatttgtc
     5641 ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct
     5701 agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt tgaagtccca
     5761 aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt
     5821 tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc ttgttccttt
     5881 aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc
     5941 caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt
     6001 ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac
     6061 atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag
     6121 taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata
     6181 gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga
     6241 actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga
     6301 tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat
     6361 aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt
     6421 tacaacaaac tggtcctgac acgctcgacc cttgaaatga gtcaaagcct tgttgacaat
     6481 ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac gagcgccatt
     6541 agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat
     6601 ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt
     6661 ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt
     6721 cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc
     6781 cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa
     6841 ctggtcacga tgactgccat cctctataaa atcttccaaa atataagttt ggtaatgata
     6901 caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg
     6961 agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag
     7021 aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa ttcgttgatt
     7081 ggaaatgaag ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt
     7141 ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc ccaaatcgat
     7201 catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat
     7261 ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt caaccttcat
     7321 cttatcttcg tttaaatctg aaagataacg attatagtct tcctgagaaa tatactgctc
     7381 aattccattg aaaccatagg aataggctaa caatgcaacc ttgataccat tcacttcctt
     7441 aatgaccagc ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc
     7501 agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg
     7561 attatgagct aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac
     7621 aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa
     7681 atcaccaata gctaaatctg ctgcttagtc cccttgcttg agccaaggag tcacatactc
     7741 aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga aaagtccatc
     7801 gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt cctactcctg
     7861 agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag tcaagggatt
     7921 ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc tagcaagagt
     7981 atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt accaagccct
     8041 tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt atcttcgttc
     8101 tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt atttctttca
     8161 caattgaatt gaacttccta tctattttct ataaatccta aatgccataa tgctttcaat
     8221 tcttgtcatt ttgtgatatc atgtagaggt aatgaaagga aatgaactaa tccacagtgg
     8281 cttattccaa gtataccact tgggctttgg cagtagctaa ctgcactaaa cataatataa
     8341 ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat tgatatgaac
     8401 gatattatcc gtgaagggaa tcctactcta cgcgcgattg ctgaggaagt cactttcccc
     8461 ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa acattcccaa
     8521 gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc tgctccccag
     8581 ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga agaaggcgaa
     8641 actccacagg aagcctacga tttggaagcc attatgtaca atccaaaaat cgtctctcac
     8701 tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg
     8761 cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa agatggagaa
     8821 aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga aattgaccac
     8881 attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc agttaaagat
     8941 ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg tgttataata
     9001 gaggcatgaa aacaaatgat attgtctatg gtgtccacgc cgttaccgaa gccctccttg
     9061 caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat gttgagaaag
     9121 tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa aaatctctct
     9181 ctgagattac tgaaggtgct gttcatcaag gttttgttct acgagtgtct gaatttgcct
     9241 atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaatcca cttctattga
     9301 ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca gccgatgcga
     9361 ccaatgtttc aggtgtcatc attcccaagc accgtactgt cggagtaact cctgtcgttg
     9421 ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtgacc aacctcagtc
     9481 aaaccttaga taaacttaag gatgaaggtt tctggacctt tggaacggat atgaacggta
     9541 ctccttgcca caagtggaat acaaaaggga aaatcgccct catcattgga aatgaaggaa
     9601 aaggtatctc tagcaacatc aaaaaacagg tcgatgaaat gattaccatt ccgatgaatg
     9661 gacatgttca aagccttaat gccagtgttg ctgcggccat tctcatgtac gaagttttcc
     9721 gaaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat gattaactat
     9781 gttctgtaat gaatttatag gcttcttgac cagcgatagc tccatctcca accgctgttg
     9841 ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg tcgactgcag
     9901 ttttcatgtg gttatctgtc acaatccatc ctgcctgatc ttggatattc aattctttaa
     9961 caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag gcttgttctg
    10021 tcacttgacc tgttttcaca ttttcaaata cgactgattc tactcggttt tcacccttga
    10081 tttcccttac tacagaatcc cagataaagc tgattttttc attcgcaaag gcgcgatctt
    10141 gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca gtcttagcaa
    10201 aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact accaataaat
    10261 cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca cgactgttca
    10321 gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct acgataactg
    10381 tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca tggtcttcga
    10441 cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt tcaaacatct
    10501 tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg atatcagatg
    10561 tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt agattgcttc
    10621 gagcagcata caaggccgca gtcatccctg caggtccagc accgataata atagtatcgt
    10681 acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt cttgtcaatc
    10741 acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg ataatactgg
    10801 gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag ccctggctgt
    10861 cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag
    10921 gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta gcatgatttc
    10981 tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg agttttcctt
    11041 tatctattat atcaaatata agtccgtttg taactagcga agaattcttt tgtccgctct
    11101 tctttagggg tgtggataat ctcatccgga gttccagact cgatgatttt ccccttatct
    11161 aagaagagaa ttttatccgc aacttgggct acaaaggaca tgtcatgact gaccaaaatc
    11221 atggtctgac ctgacttagc agcatctgca atagactttt ctacttcacc gaccaattct
    11281 gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat agcaagcgca
    11341 cgcgctaggg caacccgttg cttctgtcca cctgataaat ggcgaggata atggttttca
    11401 cggtccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc ttcttggtca
    11461 gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt tcggcgttca
    11521 aacaaattaa actgttggaa aaccatagac aacttacgac gtagggcaag gatttcttct
    11581 tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc actgtcaggt
    11641 gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga agaaccaatc
    11701 aaggctacaa cttccccttt ttgaatatcc aagtccagat gatccaagac agtctgtcct
    11761 gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct ttcacatctg
    11821 tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga ccgaggcttt
    11881 caattccgat attgactacc caataaacaa gggcaacgga gatgaagcgt tcaaaatagc
    11941 gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca cccgcactaa
    12001 aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta
    12061 ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc atacccagac
    12121 tgcgtgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga atggtttcac
    12181 tagcataagc tgcctcatta aaggcaaagg cgacaatcgc aaaagctgca gctggaatcg
    12241 cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc aaagggattc
    12301 cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga
    12361 aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca aaaagaagcg
    12421 ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt
    12481 ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg ccatccggaa
    12541 tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca ttcataaaaa
    12601 catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa attatttgct
    12661 acggtcagat acagactttg tctacctact agtttatcat actttgaaac agggaagtta
    12721 tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc ctcataagat
    12781 tttcgataac cgatttgctt aacagttcca ttttctacta aaatgggccg ttttaacaac
    12841 ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc taccttatct
    12901 tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt caattcaaat
    12961 cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc cacgatatgg
    13021 acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt acaagttgaa
    13081 catttggggt attcgataaa ttctaacatg tgcttctcct tctattgctt ctatctttaa
    13141 aatctactcc ttcatgctcc aagagccaag ctttcttttc cactcctgca gcataacctg
    13201 tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac caaggattgc
    13261 gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga gcaatttgtc
    13321 cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact ctcttttcaa
    13381 aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg cctttaaagt
    13441 aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact tcttctatcg
    13501 tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt ccatacaaat
    13561 aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagg atttttacat
    13621 acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat ctactggcat
    13681 cttaccgact tgtttaaaac caagtttttc aaaaatatgt tgcataacct tgttttcagc
    13741 atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct ctaagaaggt
    13801 ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa agactagata
    13861 ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct cctctggact
    13921 cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt aggcttgccc
    13981 tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc actggtcact
    14041 accagcatct gctaaacatt ttttggcatc ctccatcacc tgcatgatgg catctacttc
    14101 atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag tttctcccat
    14161 catagcataa tttaagtatt tctacaagca gttaaaactt gactttgtgt ttttgttatt
    14221 ttataatcta gttatcaaaa ctatataaaa aggagttaaa aatgaaaact accttttcct
    14281 acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt ttgctctatg
    14341 tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca gcatctatgg
    14401 tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa taccaacgcc
    14461 aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct attcaagaaa
    14521 tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc tacgatgctt
    14581 ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa accagctgcc
    14641 aaaccgttaa actctatcat caaactctag acttaatcga tcatactcaa gaggaggtaa
    14701 tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg agattgctaa
    14761 tagcgtgacc catgctgtgg gtgcagtcat catgcttatc ttgctgccta tttcatccat
    14821 ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt tcgtcatcag
    14881 tctctttctc atgttcctat catccaccat ttatcactct atggcctatg gttcgaccca
    14941 caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg ccggctcata
    15001 cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta ttgtcatcca
    15061 atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg tcaatgagaa
    15121 atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca ttcctgccat
    15181 tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac tctgttatac
    15241 agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt ggcatctctt
    15301 tatcctagct gcgtccgcac ttcaatacat cgctattgtt tattacatgt aaaaaagttg
    15361 agaaattcaa tctcaacttt tttctttaca catattgata aagtactggt gcaagcgcac
    15421 atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt gggctgcaac
    15481 aatttgattg ttcactgttg ctaaaatttc tacaccctca ccaacactac tgataatcgg
    15541 accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt ccgtgtagaa
    15601 acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag ttcctagatg
    15661 actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac aggtcccaaa
    15721 cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct ggtcacgtaa
    15781 gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca agtcactctg
    15841 atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta attgatctag
    15901 cacttttgca tgttctgcaa aggccccttg caaggccaat attccgattt tcatctattt
    15961 tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca tggcttctcc
    16021 taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca cagccttaac
    16081 aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac cgacaaagac
    16141 cccctctgcc cctaattgca tcattaacgc agcatctgct ggcgttgcaa cacctccagc
    16201 agcgaaattt acaaccggca attttccatg ttcatgaaca tattggacca attctacagg
    16261 gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt gaatgcggcg
    16321 aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc ctgtccctgg
    16381 ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg cttcacccaa
    16441 atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat ggaaacggtc
    16501 atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag cctctaaaat
    16561 ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac taaccgcttc
    16621 ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag ctgcacgaat
    16681 atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt ctgcgatacg
    16741 agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct gtgccaagtt
    16801 tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt gtattggtag
    16861 ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac tataattgta
    16921 tggggacaaa ttagctaaaa actatctgac cttaacttaa ggccagatag ctcattcatt
    16981 tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggttta ttgaagtgtg
    17041 gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct tggatagcaa
    17101 gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt tgggcaccaa
    17161 gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg ttgtcatgtt
    17221 tcatgaattc tggtttttga agatcgttaa agcctgtttc agttgcgttg taaccagcag
    17281 ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag attgagatac
    17341 catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcattg taggcaccaa
    17401 cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct ttacgagcat
    17461 tgtcataaac agtcgcacag tcaccaacag cgtaaacgtc tgggatagat gtttcttgtt
    17521 tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccacct gcaagggctg
    17581 tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt tctttgtcag
    17641 taatcaagcg ttcaacttta ccgtcacctt cgattgcttt aacagtttga cctagagcca
    17701 agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct ttgtcatagt
    17761 aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca agacgttcaa
    17821 aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata cggtcgaggt
    17881 gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac aatttcacga
    17941 attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt tcaacacctt
    18001 cgattggtgg caagattggt gtagagcctg tagcgaaaat caatttttcg tatgattctt
    18061 tgtgctcttt tccttcaact tccgctgtaa ctactttgtt atcatagtcg attgaaagaa
    18121 caggtgagtt catgtaaact ttagcacctt tagcttccaa tttttcttta tcagaataga
    18181 acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca catcctagga
    18241 aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca aaattatcca
    18301 acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg attttactca
    18361 taaaaatttc ctgccttcat tctaaattta ccttactagt ataccattta ccgttagcgt
    18421 ttacaagggg ggtactatta tttcagcatt ttttatcaaa aataattccc catttatcct
    18481 gttttcaagc ctgatgaatt ttatgagcat atttcttgac tttttccaaa ttttgtttgt
    18541 gaaaattcta actttatggt attataataa catgaaaatg aaatgtaagg gcttcaattt
    18601 agatgattat ttgatattac aacttatttt ctataagaga aaggagttgg tagcttgact
    18661 cttcgttcac attctgaacg gctaatgggg actactatca ctatttcatt agtggatgag
    18721 caagccgata tctttctcca aaaatccttt gacttgctca aagaacttga ataccgcttc
    18781 aacgccaata gtcaagaatc tgagttgatg gaaatcaatt atcaagcagg aatagccccc
    18841 gtcacggttc atccagacct gtttgagctg atttcacttg ggttagagca tagcctagcg
    18901 ctctctagcc acctcaatat cagcattggt cccttaattc aaacctggcg tatcggtttt
    18961 tcagatgcca aggtcgccca gcctcaagaa attgaatcgg tgctgcctct aatcaatcct
    19021 catggtatcg agttagattc ttctacttct actgtgtttt taaaacagaa aggaatgaag
    19081 atcgatctag gttgtttagc caaaggatac agtgcggata aggttgccca atttcttagg
    19141 aaagaggggg tgacttctgc cttgatcaat ctgggaggga atatcctgac cattggaaaa
    19201 aatcaggcaa gaggggataa cccatggcaa atcgggattc aagacccagc caatcctagg
    19261 ggaaatcatt taatgaccat ccctgttgtc aataaatctg tcgtgacttc aggcatttat
    19321 gaacgtcacc tgaccgtcga tggacaagat taccatcaca tttttgacag tcaaacagga
    19381 tatcctgttg aaacggaact agcgagtcta acaatcatct ctgataaatc agtcgatggc
    19441 gaaatctgga cgactcgact ctttggagaa agacctgctt ctatcctctg gcaagtcgaa
    19501 agtttggagg gcatcgaagt catcctcatc gataaagaag gccacctaag ctgttcttca
    19561 ggaattccta ctctatagag acaactgttt caatggaatt ttaaaattgt attatgtaaa
    19621 aagagaaagg aaatctcatg ttaaaactta ttgctattgt tggaacaaat tcaaaacgtt
    19681 ctacaaaccg tcaattgctt caatacatgc aaaaacactt tactgacaaa gctgaaattg
    19741 aacttgttga aatcaaggcc attcctgtct tcaacaaacc agctgacaag caagtacctg
    19801 ctgaaatatt ggaaattgct gctaaaatcg aagaggcaga tggcgttatt atcggtactc
    19861 ctgagtatga tcactctatt ccagctgttt tgatgagcgc tcttgcttgg ttgtcttatg
    19921 gtatttaccc acttttgaac aaaccaatca tgattacagg tgcttcttac ggtacgcttg
    19981 gttcatctcg tgcccaattg caacttcgtc aaatcttgaa tgctcctgaa atcaaggcaa
    20041 atgttcttcc agatgaattc ttgctctcac actctcttca agcatttaac ccaagtggcg
    20101 acttggttga ccttgatgtt atcaagaaat tggatgccat ctttgatgac ttccgtatct
    20161 ttgtaaaaat cacagaaaaa ttacgtaatg cacaagaatt acttcgcaaa gatgctgaag
    20221 actttgactg ggaaaatttg taagatagga gaccaaaaag aatgaaattt gttggacttg
    20281 ttggatcaaa ctacgatcaa tcatataacc gtaagctctt ggaatttatc cgtcgctact
    20341 tcaaactcaa atttgaatta gaagttcttg aaattgacga agttccaatg tttaaccaag
    20401 atgaaaaatg ggatgaaagt ttccaattac gttatttata taacaaaatt actcgtgcag
    20461 atggtgttat cattgctact cctgagcata accacactat ctcagcttca ctcaaatctg
    20521 tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg
    20581 gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg
    20641 atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta
    20701 aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa
    20761 cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac
    20821 caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg
    20881 accctgacga tccagaatgg gtagaaaaag ttgcagcatt cactggtgct gtttctggtg
    20941 atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga
    21001 aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca
    21061 acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc
    21121 gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta
    21181 tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg
    21241 caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg
    21301 gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg
    21361 gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg
    21421 ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag
    21481 acgctacagc cggtgcaagt tactaatgat agtttttagg aaccattatg gttccttttt
    21541 tagcgaaaaa agactagcaa acacaaactg ctagtctctt tgttatcgtt tcatctatct
    21601 ttgttaaaag aaagaataca tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    21661 cgcaggctgt acttaagtac ggcaaggtga agctgacgtg gtttgaattt gattttcgaa
    21721 gagtattatt atgaatttgg atcatcaaga ctacggccat gtaatccttt ttcacgttgt
    21781 acttggcgta gtttttctgg tgtaacatcg tttccttctt cgtccacaat tttgattcct
    21841 tcaatgtggt gacgaacagc gcggcgataa ccttcgatgt actcctcacg tagtttggct
    21901 tgttccactt tttcttctgg tgttaagcct tctgtttttt tctttttagc aagctcattg
    21961 atacgagcaa tttttttcgg atccatttct tctcctttgt aataagataa agtccgttta
    22021 tcaaatttta tttagtgtta atttggttaa aacaagcaaa cttagctgct ttcttggtta
    22081 gttgtgacaa gattgccaaa cgattttgac ggacagcctg atcttcagcc attaccatag
    22141 tattttcaaa gaaagcatca atgactgggc taagcgcaaa aagttgtttc aattgctgac
    22201 ttgcaggtcc tgataaaatg agtgtttcta ctgcttctgc caaagctttt tcttggtcat
    22261 tctcaaatag tgctgaatca accgtagcaa ccccttctgc cttctcggcc aggttaaagg
    22321 cacgagaaag tgattcaaca gatggtttaa aatcttcttc cttgcttact tctacgagag
    22381 cacttgctgc ttccaacata tctgccacaa caaagtttga acctgcaaga actgcttcct
    22441 tgatatcttt tggagtagag cccatcatct tatcaacacg agccttgata aagtccataa
    22501 cctctgcttt attttcataa gtcaaactgt caaatttcaa tgcataaagg ctatcaatca
    22561 gctcatccat agcaatgtgc caaccaaagg catccaagat acgaaccaca ccttgagttg
    22621 cacgacgaag ggcataaggg tcattagaac ctgatggaat caatcctact gagaagaaac
    22681 tcaaaatcgt atccaatttg tctgcaatgg ctagaactgc gccgaccttg ctctctggaa
    22741 gttctccttc agctgatgta ggcatgtagt gttcacgaat agcagctgcc accgctggag
    22801 tttcaccagc aagaagggtg tatttttcac ccataattcc ttggagttcg tcaaattcac
    22861 caaccatacc tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca aggtcaactg
    22921 tttcatccac tgacaaacta gctttttctg ccaaaagtac agtgatttga cccgtacgaa
    22981 tcatgtgttc acgaagagaa ccaatcttct catggaaggt gacattgttt aatttttcaa
    23041 caagatctga aatcaccaat ttttggtctt cacgccagaa gaattctccg tcttccaagc
    23101 gggctaccaa gactttttca tttcctttga tgacattttt caaacgctct gcgtttccgt
    23161 tacgaacaga aatgaagttt ggcaagagtt ttccatcttg atcacgaaca acaaagtaac
    23221 gctggtgttc cttcatagaa gtcaccaaga cttcttctgg aacttcaagg tatttagcat
    23281 caaaacttcc catgaaggca gttgggtatt caaccaaatt caagacttca ttcagcaaat
    23341 ccgcatcaat ttcgatacgt acaccatgtt ttgcctcaat ttccttgatt tggtcaacaa
    23401 tcatttgctc acgttcacat ggatctgcga ttacaaactg cttacgaagg tcttcttcat
    23461 agctcaatgc tgactgaatc ttggtttctt gtcccaaaaa acgatggcca cgactcacac
    23521 gacttccctt gatatcaagg aaatccaagt caaactcttg ctcatccaag agaacagtta
    23581 aagtgtgaac agggcggatg tattcaaagc tatttcccgc ccagtgcatg ctgacaggga
    23641 aagtcagtga cttcaagaca tccacaatgc ctggaacaat ggcttcaact gcttgaccaa
    23701 tttcttcctt agtgacatag acatattctt cacccttgat ttcacggaat tcgatatctt
    23761 caacagtcaa acctttccca cggacaaatc cttgagctgc tttggtgaag tttccatcac
    23821 tatctaaggc aattttcttt gctggaccct tgaaatcttc tgttaaatca gactgtttgt
    23881 ctgcaagacc agttacacga acagccaaac gacgtggtgt tgagaaagtt tgaatggctt
    23941 caaaagacag gcgttttccc ttgaggaagg ctgccatttt ttcgcctagt tgtttttcac
    24001 ttggcgtaac aacataggct ggtaattctt caagaccgag ttctactaat aagttttttg
    24061 tcatgttttt tctccgaaaa gtatcttttt attttccagt ttgccttatg tgattggcac
    24121 gcaagcaacc aacttcgttg tttcattgct aaaattggag ccaaaagtct ccaattttgc
    24181 ctagtatcct tagtttcact aaggatacct tcatcactgc ggcaactggc tcagggctca
    24241 ctctgttcgc ccattttcgt aatttgtcac tctctttatt ctgcgtcttc tgctaggagt
    24301 ttagctcttg tttcttcatc caaaagtggg tagcctaggc gtttgcgttc tgcgacaaag
    24361 gttttggcta cgacacgggc caagttacgg atacgagcga tatagcctgc acgctctgtt
    24421 acagatacgg caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt gagaacatag
    24481 tcataggcag ggtgtaccaa gccttcttct aatgcacgac cagcttcttt ttcaaactta
    24541 tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa atgaatattt tgagtgctca
    24601 tactcaggct ggataaagat ttctccgtat tttacaccat cagcccactc gatatcatag
    24661 acagagtcta cttcttgaat gtaagaagcc aagcgctcca aaccataggt aacttccgca
    24721 gtcacagggc cagttgccaa tccaccgact tgttggaaat aagtgaactg agtgatttcc
    24781 attccgtcaa gccaaacttc ccaaccaaga ccagctgaac cagttgatgg gttttcccag
    24841 ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga ttcccaattt ttccaaagac
    24901 tcaaggtaaa gttcttggat atttgatgga gaaggcttca tgaccacctg gaattggtgg
    24961 tgttggtaga gacggttagg gttttcccca taacgaccgt cagcaggacg acgtgatggc
    25021 tctacataag ctgcattcca tggctcaggt ccgatagcac gaaggaaagt gtaaggactc
    25081 attgtccccg cacctttttc attatcataa gcctgcataa gcatacaatc ttggtcattc
    25141 caaaattgtt gcaaagtcaa aataatttct tgaaatgtta atttcttaga cattgaatac
    25201 tccttaataa aaaataattt cttgcgacac gagtctgcct cgtcgattat acgaggcaag
    25261 acgagttagt actgcgtcta gctcaggcac tctagtgctg agcgtagggc agtccgactg
    25321 taaggaggtt tctgccactg acgcagtgaa atgttaattt cttagacatt gaatactcct
    25381 taataaaaaa taattacttg cga
//
LOCUS       DAVHEW010000016        25370 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVHEW010000016 DAVHEW010000000
VERSION     DAVHEW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.016"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..312)
                     /locus_tag="VVL53_000669"
     CDS             complement(<1..312)
                     /locus_tag="VVL53_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182876.1"
                     /translation="MYLDGGAYLSKSACQGICAALAMSPGTFIALTGAAVLTKKLINY
                     IKVGGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFKASKVMKRRIFSLFVLDL
                     MV"
     gene            complement(341..679)
                     /locus_tag="VVL53_000670"
     CDS             complement(341..679)
                     /locus_tag="VVL53_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU4182877.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            1145..2008
                     /locus_tag="VVL53_000671"
     CDS             1145..2008
                     /locus_tag="VVL53_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4182878.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            complement(2195..2665)
                     /locus_tag="VVL53_000672"
     CDS             complement(2195..2665)
                     /locus_tag="VVL53_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU4182879.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVWAVESYVIDVKDEFATDYVLVAHQSHAYYEQKY
                     LLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(2631..3437)
                     /locus_tag="VVL53_000673"
     CDS             complement(2631..3437)
                     /locus_tag="VVL53_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU4182880.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATKLGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKKLKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(3644..4285)
                     /locus_tag="VVL53_000674"
     CDS             complement(3644..4285)
                     /locus_tag="VVL53_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182881.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(4282..6390)
                     /locus_tag="VVL53_000675"
     CDS             complement(4282..6390)
                     /locus_tag="VVL53_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4182882.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPNTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6442..6729)
                     /locus_tag="VVL53_000676"
     CDS             complement(6442..6729)
                     /locus_tag="VVL53_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU4182883.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            7160..7480
                     /locus_tag="VVL53_000677"
     CDS             7160..7480
                     /locus_tag="VVL53_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU4182884.1"
                     /translation="MQRLEVYKNYQRLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVKIYYQ
                     AKER"
     gene            complement(7532..8119)
                     /locus_tag="VVL53_000678"
     CDS             complement(7532..8119)
                     /locus_tag="VVL53_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4182885.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            8361..9032
                     /gene="sdaAB"
                     /locus_tag="VVL53_000679"
     CDS             8361..9032
                     /gene="sdaAB"
                     /locus_tag="VVL53_000679"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU4182886.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            9041..9913
                     /gene="sdaAA"
                     /locus_tag="VVL53_000680"
     CDS             9041..9913
                     /gene="sdaAA"
                     /locus_tag="VVL53_000680"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU4182887.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            9935..10567
                     /locus_tag="VVL53_000681"
     CDS             9935..10567
                     /locus_tag="VVL53_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4182888.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            10617..10874
                     /locus_tag="VVL53_000682"
     CDS             10617..10874
                     /locus_tag="VVL53_000682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182889.1"
                     /translation="MKIKEQTRKLAAGSSKHCFKVVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHIRQGDVDVV"
     gene            complement(10960..12810)
                     /locus_tag="VVL53_000683"
     CDS             complement(10960..12810)
                     /locus_tag="VVL53_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU4182890.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(12849..14009)
                     /locus_tag="VVL53_000684"
     CDS             complement(12849..14009)
                     /locus_tag="VVL53_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4182891.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            14067..15233
                     /locus_tag="VVL53_000685"
     CDS             14067..15233
                     /locus_tag="VVL53_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU4182892.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            15372..15698
                     /locus_tag="VVL53_000686"
     CDS             15372..15698
                     /locus_tag="VVL53_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU4182893.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            15685..16278
                     /locus_tag="VVL53_000687"
     CDS             15685..16278
                     /locus_tag="VVL53_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU4182894.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            16271..17182
                     /locus_tag="VVL53_000688"
     CDS             16271..17182
                     /locus_tag="VVL53_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU4182895.1"
                     /translation="MRKWTKGFLIFGVATTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSANHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            17245..18309
                     /locus_tag="VVL53_000689"
     CDS             17245..18309
                     /locus_tag="VVL53_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182896.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEERAQEVADKAEETIANLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIEAPEDSEARTGIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(18390..19241)
                     /locus_tag="VVL53_000690"
     CDS             complement(18390..19241)
                     /locus_tag="VVL53_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU4182897.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDVPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLA"
     gene            complement(19516..20502)
                     /locus_tag="VVL53_000691"
     CDS             complement(19516..20502)
                     /locus_tag="VVL53_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU4182898.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(20774..22249)
                     /locus_tag="VVL53_000692"
     CDS             complement(20774..22249)
                     /locus_tag="VVL53_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU4182899.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(22507..23430)
                     /locus_tag="VVL53_000693"
     CDS             complement(22507..23430)
                     /locus_tag="VVL53_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4182900.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(23444..24373)
                     /locus_tag="VVL53_000694"
     CDS             complement(23444..24373)
                     /locus_tag="VVL53_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4182901.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(24692..25042)
                     /locus_tag="VVL53_000695"
     CDS             complement(24692..25042)
                     /locus_tag="VVL53_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182902.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTAAELI
                     DNLKEVIAKLKANA"
     gene            25122..25259
                     /locus_tag="VVL53_000696"
                     /pseudo
     CDS             25122..25259
                     /locus_tag="VVL53_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         7686 a         5456 c         4545 g         7683 t
ORIGIN      
        1 gaccatcaaa tctagtacaa aaagagaaaa tatcctcctt ttcattacct tacttgcctt
       61 aaagattctg tttaatagaa cctttatcgt agtatctaca caaattttta tcaaaaagat
      121 aagagccaat acacctgctg ctgcaccaat aagccaacct ccaaggcctc caaccttaat
      181 atagtttatt agtttttttg ttaaaactgc agctccagtc aatgctataa aagttcctgg
      241 gctcatagct aaagctgcgc aaattccttg acaagcactc ttgcttaaat aagccccccc
      301 atcaagatac atcatctcct cttgctcaag agcaacataa ttatttggta atacgagttc
      361 catttatttt ctcctttata ctatgtaata ccacaaaatg attttgacct aaatcattct
      421 taacttgaag aatcgcagga aatgttaatt ttggagtcaa atcttccagt gccactcgaa
      481 cagcttttac tgtaaaattc aacttattca gacctgaaac aataccactg acagtcgttc
      541 catacatgtc tgtaccaata atttcccgaa tcttcatgat tgatagttcc tttttgtaag
      601 ataaaagaac tgttgaaaca actgccgcag cacaatctga ctgatcatgt tgcatcgtac
      661 aaggatattt ttttaacata gaaatactca attctttaga attaatctaa cttttctttg
      721 atgtcaccca aacctcaatc actgcccagt agttcttgaa tcagtttatt ttcaatcgta
      781 tcattctttg ttcttgatat ctttccaagc attgtagtac agcagatttt tgtccaagga
      841 ctaagcctcc tttaacaact caagctccct tacattatat cgaccagccc catggaaaga
      901 ttatggccag actaatcaat gatatcatac atactagaat acaaaatatt tttttattca
      961 tactttttct cctatcttta ttttctacct acatcatacc tcttattaag ctcattttaa
     1021 aattacgtta acttttttca caaatttatt tttactttct tttttacttt ttacactgct
     1081 atgatacaat atttttaaca agattttatt atttgttaac ttttttcaca gaaaggagaa
     1141 aatcatgcgt tatgatttcg gaaaggtcta taaagaaata cgtgagtcaa aggggctgac
     1201 ccaagaagag gtctgtgggg gtgttctctc aagaaccagc ttatcaaaaa tcgaaagtgg
     1261 caagacaacc ccaaaatatg aaaatatgga atttcttctc cgtcaaatca atatgagttt
     1321 tgaagagttt gaatatatct gtcaacttta tcaaccaagt caacgcacag agattatgca
     1381 aacctatctc aatatgcgtt ccattatcgg gaccagcgat cttgtcaatt tatttcaaaa
     1441 atgccaagac tacctcaaga cccaccacga tctccctata gaagaaatca gagatatgct
     1501 tgaagttgtc atttatctcc gtcaacacgg ggcaggggaa ctatcagatc acgcagaaca
     1561 ggttgtcaaa aaactttgga gaaaaattga aaaacaagat acatggtatg agagtgatct
     1621 aaaaatcctc aataccattc ttttcagttt tcccattgaa tacctccatc tcatcactgg
     1681 aaaaatattg caacgcctag aagtctataa aaactatcaa catttatatg acttgcgaat
     1741 agcaatcctg ctaaaccttt ccacactcta tctatacaat caagataaaa acatgtgtaa
     1801 gcaaatctgc tacactttac tggaggacgc caagaataag aaaagctacg ataggctggc
     1861 tatctgctat gtccgcattg ggatttgtac agacgattct aaacttatcc aaaaagggtt
     1921 ctcccttctg gagctgaccg aggaaacttc tatgctgtct catctcaaaa aagaagtaga
     1981 gacccattat caaccaaaga aattataaaa aaagtcgagg gagctcctcg accttttcat
     2041 agaatcgccg aacgatttaa cgagaaagta tgacttttac gtttatccca actcaattat
     2101 gacatttttt tcaaaagtca atatatctca ctttttcaac gacaagaaag aggctgataa
     2161 tctaccaacc tcttattctg aacccatcac tccatcactt tttagcttca ttcgctttct
     2221 tagcgactgc aatctggtat tcgacttggt cattcccctt accggtacaa ccatgagcaa
     2281 ttgtagtcgc tcctatctga tgcgctattt caaccaattt tttagaaatc agagggcggc
     2341 tcaaggcaga taccaagaga tacttttgtt cataataggc atgtgactga tgagccacta
     2401 gcacataatc tgtagcaaat tcgtccttaa catcaatgac ataagattct actgcccaaa
     2461 ccttaagagc cttatcatgg atgaaatcta agtctttccc ttcacccaca tccatacaaa
     2521 ctgaaacaac atcatagtct ttctttaacc aggtaatagc aactgatgtg tccaaaccgc
     2581 cagaataggc caaaattact ttttctttac tcatgttctt ttccttcttt ctatttttca
     2641 atggatgctt taaaggcatc ctcaatgagt ttgtctaatt ccccagactc cttcaacttt
     2701 tgaatggttt tatcgactgc ctctttcaat ttcttgctat cttttttcat ggctaccgcg
     2761 taggaatcat cttgctcttt ttcaaaattg aggtctgcga ttgctaaatc aggattattt
     2821 tccacaaatc ccttggaaac aggttcttca aagataacgg catccacttg tcctgatttt
     2881 aaatctgtga ttaaattccc atttttaggc agagatacga gggaagaatt ttgtagcaaa
     2941 tctttcgcca tcgtctcttg aatcgaacct ttctgcgctc caaccttttt ctgcgccaag
     3001 tcgtttacag actgataagt agtcaagtca gattttttga caatgagttt attttttgca
     3061 gtatagtagg gaatggaaaa gtcaaacacc ttgctccgtt catctgtctt agaaacacct
     3121 gatatggcaa ggtcggcttt tcctgattga acactagcca gtacattatc aaaactcatg
     3181 ggagatagtt ccaattcgac acctagtttt gttgcgatag ccttggctaa ttcgatatct
     3241 gaacccacaa tctgattttt cccatcaacc actttttggt attcaaatgg agcaaaatcc
     3301 ggattgaggg ctacaaccaa ttttcctttg gacttgatgg cttcaattcc agccgattga
     3361 ttgttgctac aagcaaatag tagggggagc atcactaaac caaacatcgt catcaacacc
     3421 ttcttcatct tattcataat aaagcctact ttatgtttat tccttttatt tgtataaaca
     3481 atacatctat atgcatcctt tgtcaagcct aagttgtaat ttttttcaat ttaaaacaga
     3541 aaaacccagg aaaatgacat aaaaatatca ttcctaggcc tatttatgct atttctctct
     3601 gaaaaatatg agtattcagt cggtcaaatg aagctgaacg aactcatttt ccctcgccta
     3661 attcaatgat tcgatgacat tgttgggcta cataagcatc gtgggtcacg ataatgactg
     3721 ttttcccctc tcgattcatc tctaagagaa acttcaagac caaatctcta ttttcaggat
     3781 ccagagaacc tgtcggttca tcggctaaaa tcagctggct gggttttaag atggctctag
     3841 caactgcaat tcgttgttgt tcgcccccag acaactcgga gaccctttga tgcaaagtag
     3901 ctgataaacc tactctctct aaaatctctt ccaccttttt gagcttgtct ttcttaggca
     3961 atttcacata tttcagcgcc agcatgagat tgtactcgac cgtttcatca tcaatcaggg
     4021 caaaattttg aaacagataa gagatatgtt cacggattat tgtttgcgac ttagcagaat
     4081 taaccgctag atttgtctga ccaaaaatct cataccgtcc gctataatca ccatctatca
     4141 aacccaataa atttaacaag gtcgacttcc cactaccact cttaccaaca atagctacca
     4201 aatccccctg atcaatcctg agagataagt tatccaaaat cacttttccc ccaatggttt
     4261 tggtaatatt tttcaactca atcataagat gccccctttc aataactcta ctagacttct
     4321 tttctccatc ctagaagcta agcctagcac aaatagtata tccagacatg taaaacctgc
     4381 aaacagtaga agtggtaaga acgcatgggc aaagaaaatc aagactagaa gagggaaact
     4441 atagcccagc aagagcagaa cgaggagagg acggtagcga tcgaccagtt tccaccccat
     4501 aaacttcttg gtaatgatat ccctgcgctt caataagaaa gttgttacta gtaagaagta
     4561 ggaaatcatc atgctaagga gaccaaacaa agcaaagagt aggttaaaat tccgaacagc
     4621 atctcgataa gaatccactt tctcttgttg aatggcttga atagatgaaa attttaaata
     4681 atttccatct gacaatttct caactaactc tgtaatctct ttttgatgtt gaaccgtatt
     4741 ttcaatttta atcggattat ttaagccagt tgttgacagg gaggctttct catcccacat
     4801 catatcagaa tcattgacca agctaataat tggattggag agattttcct ttcgcttatc
     4861 actatatggg aaaaatgacc aatctccttc ataataggca atctcgacat ccatctcctc
     4921 tatcgttcgt ttttgctgct cttcatactt catcgaatga aaggcaatta acttccccaa
     4981 gagctgattt ttatcttctt cacctttcgt acttgctggc atcaaaataa cttttttaat
     5041 accggtattt ggtagcttga atcccttgct ctttagaaaa ttgcgattgg catagtaaac
     5101 atccaccgta tctgttaact gatattgctg aatctgttct gattggacaa aattttttac
     5161 aggaagactg ctactctgca catagcccgc ctgcgttttt tctaccaaat cctgataaaa
     5221 tcgatagaaa taatctgtag atttccctga ccctgctagc tcttcttgcc acagattatc
     5281 attgagtttg aaggtttcta aggtcaggta attaccttga cttacccact gttgctgata
     5341 agcaagttct ttgttttctt gttctaaact tctgcccacc ccaatcagta aggccgtcag
     5401 taaaatagtt gtccctattt tcatcacata attgaagata agaccaaatt tgaaagatga
     5461 aaaacctttc agcagagagc tgattgtcat tttttggatt aagaggtaag tcaaccaact
     5521 gataaagaga taaagctgca acagcaaaaa atgagacaac cacagcatag gaaacaaatc
     5581 ttttggctta taatcaagca agaaaaacac gcctagattg atcacaagag ccccacctag
     5641 gaggaggtaa aggttgcctt ttacaacatc agctaaaaca gccctatctt gaaaaccaag
     5701 taatttttgt accccaactc ttttcatctc catcatcggt tgatacactg tcactaacac
     5761 aagaagcaaa atagccaaga caaaaacaat ggcagataaa agcaaatctc gatttatgac
     5821 ttccactgca cttttgtagg tcggctctag caaggtagcc tggtctatct tgaaaaaatc
     5881 gctccatttc tgtacaatcc tatccttgtc catctcttgt gtagaagtta tcgtatagcg
     5941 accatttaaa ctacgagatg tatccttgat ataggtttga aaagtcataa gctgaatagg
     6001 tttggctttt agaaaggtcg gaatcgtacc aagtttattg gaaatttctt tattactata
     6061 gactccttca ccatctgtgg taaaatcaag agaagaaatc ccaaactctt ggtaggggaa
     6121 ggtatcttta tcaaaaacac cagacttgac cacctcatca ccactgtctg ttttgatgat
     6181 ggagacttta tactcctttg atacatcctc aaaaaatcga agaacagacg ctgcaggttc
     6241 gttaatatct ttcaaataca aatccaaaga atctacagtc tttcccattt cttgaatccg
     6301 aaccacttct cgtgtatagt ttacattaaa aacaaaaaaa gtggtacaca gaagcaggag
     6361 taacatacag aaattactga tttttttcat aaaagtgcct ccactacagt tttccctctc
     6421 caaataggag agggaagaat tttactcaaa tccatagaaa tattccattc ctgttggcgg
     6481 aatcttggtg agggaagctc tagcccatgc ctcaggtttt gcataatcct tagaggttct
     6541 acccccatgt ctaacagttg ctgtatgata tcgagtagaa tgtaagtaat cagaatatcc
     6601 aaaagttcct gtccaaccta ctccatagtt ccattgacca ccctcaaccc atactgctaa
     6661 agcatttgta gtagcaagta taccacatac cattaaagat gcaacaccaa ttttaactaa
     6721 ttttctcatg atttatttct cctattatcg agagctgttc cgtaaaaaca gctatagtaa
     6781 tttatcaaca agtatcgtag tccattggac tcaccttcct tctatctcat ttcatttttt
     6841 aacaacgaac ttactcttaa ccgggagaat ggtgcgcttc tgtatcaggt ccttgaacaa
     6901 ccagagtagt attaagcaaa ctctaactat tactcaagaa tcagtccaat aaacaatatg
     6961 gatagcgctt tcaaattttt aaactcctat cccgtccttt tatctatata ataagcataa
     7021 gcgaaaatat aataactgtc aagtatctga agtgaatttt ataaaaaaat tacaagccaa
     7081 atttgtaaag tttacactaa gccgctaggg caatcgtcta tcagaatgtc cgtttatttg
     7141 tcaataatcc gagaaaatct tgcaacgctt agaagtctat aaaaactatc aacgtttata
     7201 tgacttgcga atagcaatcc tgctcaacct ttccacactc tatctataca atcaagataa
     7261 aaacatgtgt aagcaaatct gctatacttt actggaggac gccaagaata agaaaagcta
     7321 cgataggctg gctatctgct atgtccctat tgggatttgt acagacgatt ctaaacttat
     7381 ccaaaaaggg ttctcccttc tggagctaac cgaggaaact tctatgctgt ctcatctcaa
     7441 aaaagaagta aagatctatt accaagcgaa ggaaagataa aaaagccgag ggatttcctc
     7501 gactctttac atattatttg ttcgttagtt cttaatacca gccattgtta agccagaagt
     7561 ttttagcagc agtccatgaa ccgtaacgtc ctgcaacgta ggcatctgct acacgttctt
     7621 ggttttcagc tgagtagtca ccgttcaggt atgaatctgt taattggtaa cgtccgatat
     7681 aacgtccatt tgtagctgta tagctaccac ctgattcttt ttgagcgatc cattctttgg
     7741 cttctgcttc agatccgctt acagttgaaa caactgtttc acttactact ggagtttctg
     7801 ctactggagc tgaaacagtt tcatcttgag cggctggtgc cgcataagta gctggcgctg
     7861 gtgttgcaac aggcgctaca gggccatcga taaccaactc ttgatcaaca taaatcaaat
     7921 gaatgttatc aatgtggttg ttttctgcca atttttcaac tgttgtgttg tgagtttcag
     7981 cgatttctga aagtgtatca ccttctttaa cagtgtaagt tgatgattct tgagcagata
     8041 caaatgatgg agcaaatact gcaaacaagg cagctactcc tgcaagagtt gctttaatct
     8101 ttttagttat tgatttcata ttcgaaaatt ctccttcttt ctatagttct atcataccct
     8161 ttaaatatta ccgtttcttg acacttttat gtagaaatat tacaaaatta ttttttattt
     8221 ccctaaataa ggtgtttttt gtcataaaag atactatttt tatgctattt gaggtattag
     8281 aaaggttttc atccacaatt aaagccaagc ccctcattct ttgatataat agagataaga
     8341 atttttataa ggggaaactg atgaaatcac ttcgttttca atctgtcttt gatatcatcg
     8401 gaccagttat gattggccca tctagtagcc ataccgctgg tgctgttcgt attgggaaga
     8461 ttgtctcttc catttttgat gatactccga cagaagttga attccaactt tttaactcat
     8521 ttgccaagac ctatcgtggt cacggaacag acctagccct tgttgcaggt attttaggaa
     8581 tggatacaga tgatcctgaa attccaaata gtctggaaat tgcccacaag cgtggtatca
     8641 agattgtctg gaccattcag aaagacagca atgcccctca ccctaacacc actaaaatta
     8701 ctgttaaaaa tgcccacaag accatcagcg tgactggtat ttctatcggt ggaggaaata
     8761 ttcaggtaac cgaactcaat ggttttgccg tctctctcaa tatgaataca ccgactatca
     8821 tcatcgttca tcaagatatt ccaggtatga ttgcccttgt tacagaggcg ctttcccgct
     8881 atggtatcaa tatcgcccag atgaatgtca ctcgtgaaaa agctggtgaa aaagccatta
     8941 tgattatcga agttgacagt cgcaactgtg atgaggctat cgaagaaatt cgaaaaatcc
     9001 ctcatctcca caatgtcaat ttctttaaat aggaggaagc atgttttatt ctatcaaaga
     9061 attggtcgag caagcagatc tggactttca aggaaatgtc gcagaactca tgattacaac
     9121 agagtttgaa ttgaccggtc gcgaacgtga agaagtcttc cttctcatgg aacgcaatct
     9181 ggaagttatg aaagcctctg tccaacttgg cctcaatgaa aataaatctc gtagtggcct
     9241 gacaggtgga gatgctgcca aattggatca ctacatcgaa aacggaaaaa ctctgtcaga
     9301 ttatacgatt ctctcggctg cccgaaatgc catcgcagtc aatgaacaca atgccaaaat
     9361 gggcttggtc tgtgccactc caaccgctgg aagtgctggc tgtctgccat ccgttctcac
     9421 tgctgctatt gaaaaattag acctcagcca cgagcaacaa ttggatttcc tctttgctgc
     9481 tggtgccttt ggactagtca tcgcaaacaa tgcctccatc tcaggtgctg agggtgggtg
     9541 tcaagctgaa gttggttcag cctctgctat gagtgctgcc gccttgactc tggctgcagg
     9601 tggaacacct tatcaggcca gtcaagctat tgcctttgtc attaaaaata tgctaggcct
     9661 catctgtgac cctgttgcag gtttggtcga agttccctgt gtcaaacgta atgccatggg
     9721 agctagcttt gctttcatcg cagcagacat ggccttggca ggtatcgaat ctaaaatccc
     9781 tgtggatgaa gtgatcgatg ccatgtacca agtaggagca agcatgccaa ctgcctttcg
     9841 tgaaacagct gaaggtggac tcgctaccac ccctactggt cgtcgcctcc aaaaagaaat
     9901 tttcggagaa taagcttatc aaataggaga aaccatgacc tctatcacag cgattttttt
     9961 cgatttggat ggaaccctcg ttgatagttc tatcgggatt cacaatgcct ttacctatac
    10021 ctttaaagag ctgggggtgc ctagccctga tgccaaaact attcgtggtt ttatgggacc
    10081 acctctcgaa agtagttttg cgacctgcct gtccaaagac caaatttctg aagccgtgca
    10141 gatatatcgt tcttactata aggcaaaagg catctatgaa gctcaactct ttcctcagat
    10201 tatagacttg cttgaggagt tatcgagcag ttatccactc tacatcacca cgacaaagga
    10261 tacatctact gctcaagaca tggcaaaaaa cttggaaatc catcatttct ttgatggcat
    10321 ttatggttct agccctgaag caccccataa ggcagatgtc attcaccaag ccttgcagac
    10381 acatcaacta gcaccagaac aagccatcat catcggagat accaagtttg atatgctggg
    10441 agctcgagaa acaggtattc agaaattggc catcacttgg ggatttggag agcaagcaga
    10501 tctactaaac tatcaacctg attatatcgc tcacaaaccc ttagaagttt tagcgtattt
    10561 tcaatagcat agactgggta acaattccat ttcagaagaa aatgaagtaa tactcaatga
    10621 aaatcaaaga gcaaactagg aaactagctg cgggcagctc aaaacactgt tttaaggttg
    10681 tagatggaac tgacgaagtc agctcaaaac actgttttaa ggttgtggat ggaactgacg
    10741 aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agctcaaaac
    10801 actgttttga ggttgtggat agaactgacg aagtcagtaa ccatatacgg caaggcgacg
    10861 ttgacgtggt ttgaggagat tttcgaagag tataagtctc tacataataa aacgcatatt
    10921 accaaggttg tgtaacttga tactatgcgt ttttcttttt tattcaatgt gggttgtttg
    10981 attagcaaag gcgataatag cttgcttcaa ctcatctcca ctgagagtgc ggaactctcc
    11041 aatcttttga ttgatggatt ctaaaggcat gacattaacc ttaccaacga aaatcttatc
    11101 cataacttga ttatcaacga gttcggttga taccaagagt tcttcgtaga gtttttcacc
    11161 tgggcggatt ccaacttcaa cgattggaat ttcactttca gtgtggccac ttagaagcac
    11221 catcttcttg gccaagtcat aaatcttgac tggtttgccc atatcaagga taaagacttc
    11281 cccatctttg gcataagcac cagcatggat aaccagacgg ctagcttctg gaatggtcat
    11341 aaagtaacgg gtcatacgga agtctgtcac cgttacaggc ccaccttcag caatctgacg
    11401 ttcaaagact ggaatgacac taccacggct accaagaaca ttcccaaaac gaactgcaca
    11461 gtaggttgat tggctacgtt ggttaaagcc agtgacaatc aactccgcca cgcgcttggt
    11521 tgctcccata acatttggtg gattgactgc cttatctgtc gaaatcataa ccatcttaga
    11581 cactttagct tcatcaacag ccttagcaac attgtaagtt ccacggatat tgtttttgaa
    11641 ggcttctttt ggattgcgct ccatcatagg aacatgcttg tgggctgccg catgataaac
    11701 aatagcaggt ttgtactgct caaagacttg caacaaacga tcatagtctt gaatgtccgc
    11761 aatcacaggt acataatcaa tcccttggaa cttacgaatc aattcatgat aaacaaggta
    11821 gattgagttt tccccatgac cgagcaagac aatgcgttca ggattgaagc gactaacttg
    11881 acgacagatt tcagaaccga ttgaacctcc agctcctgtg actaagatgg tcttacctgt
    11941 cagttctgca cccagacgcg attcgtcaag acggatttcc tgacgaccca aaaggtccgt
    12001 aatatcaatt ttttggaagc cagtacctgc ttggtgaagg ccctgaacaa cagtttcaac
    12061 cttaggcatc ttgtaacatt tgacacccag cttattacac atctgcaaga tacgctcata
    12121 ttctgacgga tccagcgacg gaatcgcaac gatgacacgc tcgatttgat ggcgtttggc
    12181 taattcaggc agattgtcat aagagcccaa aacaggaata ccaccaagtt tttgaccctt
    12241 tttcttagaa tccttatcca aaataccgac cagttctaat tcactggttg gatgttggta
    12301 actatccata aaaagagccc caccatcacc ggcaccaatc aagaaggtcc gacggtgttc
    12361 tccatcacca ctaccttttt tgcgtctgga gtagattaac tgccaagtaa tccgtggcaa
    12421 taaaatcaag aaggtactca acaagataaa gagaatgatg aaacggatgg agaagagtgg
    12481 caagaaggca taacagatac tatatgacaa gacactgcta gcagtcacac caaaaaagat
    12541 tttcatgaaa tccgtaatct tgctgtaacg actaatgctc gcgttcaatc cccaaaaacc
    12601 aatcatcaat tgatagaaca ggaaggccaa actcgtatag ataatgtagt caacaggtgc
    12661 tggattaatc agcccataaa ataaaatata agatacaatt atggaaacca ccatactgaa
    12721 aatatcaaat attccccaga aaacctgctt ttgttgttta tttaaaattt ccaccagatc
    12781 aatcacataa tctgttagtt ttttattcat agaaatctac tcctccttaa aagagtttta
    12841 ctatattgtt attgtagcat attttgattt atttggctta ttttatccaa ataaattatt
    12901 gttcccttgt actccttctt ccatttaaat cctctaatta aacctacaat cgtcccaaag
    12961 ccataagcaa agtgaatgga aaataaaaga aagggcatca caattagaaa tccattttta
    13021 tgttttaata aagtcagcaa agtgagtagt gacaaaagta gaaaataggc acctaatagt
    13081 aaagttatga atacgaatgt gatcggtaac aatgctagac taaacacaag actcaaaaca
    13141 aataaacaag gaacatagtg aaataatgat aaacacttaa actgaacatg acttgtcaag
    13201 ccaatccaca aaccatttaa atacttttga tgcagcattt tcttgaatgt tggtcgaata
    13261 tactgataag atagaatact tgggctatag cggattttat aaccatattc tcgaattcta
    13321 taatgaatat cattatcttc agttcggcca agttgctcat ttactaaacc aaccttctgg
    13381 aaaacctctc gtttatacat tccatgaaaa atagaagaaa catatctatc ctcagaacta
    13441 tttcgataat tggcaatgct actgccaaac atattttcct caacaagatg caaggtctct
    13501 gcccattttc cttttccttc gacaatcgtc ggtctaggcc ccccacagac aaattcacct
    13561 tgttgaataa tagccacatt gttcattaca aaagtctcag taacttttga atgagcatca
    13621 atttttaaaa taaggtcccc tacagaatgt ttaactccca ggttaaaacc actagcttga
    13681 tttttcttag gattgttata caatctaatt gagttaaact ctgtatcttc ctttataaat
    13741 tgctgaatga tagctgtggt cccatctgtg gacatagcat ttataaatag aatttcaata
    13801 ttctctttag gataggtttg attttttaag tcttcaatta gaccaggcag atatttttct
    13861 tcattgtatg cactgattac aatggatacg agaagacttt caacattttg agtcactact
    13921 actctcctga atttttcctt ccaccattct atcataattt tcaagacttt cccattttta
    13981 tcttgaaagc cagaaacctt acttgacatt atagtgaaaa agccttaaaa taagctgtta
    14041 ttaaaatcag ctagaagagg tattatatga aaaagcaatc actctttttt gttccaggta
    14101 ttatcctgat tggtgtttcc ttgcgaactc cttttactgt tttacccatt attttgggaa
    14161 atatttcgca aggtctggag gtagaagtta gttcgcttgg tgtcttgacc agcctgcctc
    14221 tccttatgtt taccctcttt tcaccatttt ctacccaact ggctcagaaa atcggcttgg
    14281 agcatctctt cacctacagc ctcttcttct tgaccatcgg ctcacttatt cgactaatca
    14341 atctgcccct gctctatcta ggaaccttga tggttggggc aagtgtcgca gtcatcaatg
    14401 tcctgcttcc tagtcttatc caagccaatc aaccaaagaa aattggtttt ctgaccacct
    14461 tatatgtaac gtctatgggg attgcaacgg ctctggcttc ctatctagct gtgcccatta
    14521 cacaagccag ttcttggaaa ggacttatcc ttctcctcac gttactctgt ctagcaactt
    14581 ttttggtctg gctcccaaat caccgctata atcatagact agctccacaa accaaacaaa
    14641 aaagtcaaat aaaggtcatg cgtaataaac aggtttgggc aattattatc ttttcaggtt
    14701 ttcaatcctt gatcttttac accgtcatga cctggttacc taccatgtct atccatgcag
    14761 gtctatccag tcacgaagct ggcttgctga cttctatctt atctctgatt agcattcctt
    14821 tttcaatgac catcccaagc ctgacaacca gtttatctac tcgcaaccgt cagctcatgc
    14881 tcactctggt ttcactagct ggtgtggtcg gcatttccat gctctttttc ccaatcaata
    14941 atttcattta ctggcttgcc atccatctcc tcatcggaac cgcaaccagt gccctcttcc
    15001 cttatctcat ggtcaacttt tcactcaaga caagcgcccc tgaaaagaca gcccaattgt
    15061 ccggcctatc tcaaacagga ggctatatcc tagcagcctt tgggccaacc ctctttggtt
    15121 acagttttga cctgttccac tcttgggtac catctgtagc tgccctcttg ctcatcgata
    15181 tcctgatgac tgtggccctc tttacagtgg acagagcgga taagatcctt taaacttcta
    15241 gttttagcta gaagtttttt atatataaaa attttacaca tttctcttga cagggctttc
    15301 ttttagatgt acaatgtatg tgtagaaaaa ttatatataa aaatcttaca cattagaaaa
    15361 ggaggtttcc catgtacttt ccaacatcct ctgccttgat tgaatttctc atcttggctg
    15421 tactggagca gggtgattct tatggttatg agattagcca aaccattaag ctgatcgcta
    15481 atatcaaaga atccacactc tatcccattc tcaaaaaatt ggaaggcaat agctttctga
    15541 caacctattc tagagagttc caaggtcgca tgcgcaaata ctactccttg acaaacggtg
    15601 gtatagagca gctcttgacc ctaaaagatg aatgggcact ctatacagac accatcaatg
    15661 gcatcataga agggagtatc cgccatgaca agaactgaat acctgactca gctagaactc
    15721 tatctcaaga aactacctga agctgaccgt atcgaagcca tggactattt cagagagctc
    15781 tttgacgatg ctggagtcga aggagaagaa gaactcatcg ctagtttggg aactcccaaa
    15841 gaagcggccc acgaagttct atccaatctt ctcgataaaa aaatcaatga agcacccgct
    15901 caaaaaaata accgacaaat tttacatatc gccttgttag ccctccttgc agcacctatc
    15961 ggcattcctc tgggaattgc catcctcgtg accctgttcg caattcttgt agccgctttg
    16021 actgtcattc tggctttctt tgcagtttcc atactgggta tcatcggcgg attcctattt
    16081 ttagttgaaa gtttcactgt cctcgcccaa gccaaatcag cctttatctt gatttttggt
    16141 tctggtttac tggctatcgg tgcttcttcg ctagttttac ttggcatttc ctatgtaact
    16201 cgcttcttcg gtctactcat tgttcgtctg gtacaatttg ttcttaaaaa aggaaagaga
    16261 ggtaatcagc atgcgtaaat ggacaaaagg atttctcatc tttggtgtgg cgactaccgt
    16321 tatcggcttt atcctgcttt ttgtaggtat ccaatctgac gggattaaga gcctactttc
    16381 catgtccaaa gaacctgtct atgatagccg tacggaaaag ctaacctttg gcaaggaagt
    16441 cgaaaaccta gaaattactc tccaccaaca cgcgctcacc atcacagact ctttcgatga
    16501 tcaaatccac atttcttacc atccatctct ttctgctaac catgatctta tcacaaatca
    16561 aaacgataga actctgagtc tcactgataa gaaactgtct gaaactccgt ttctctcttc
    16621 tggaattggt gggattcttc atatcgcaag tagctactct agtcgttttg aagaagttat
    16681 tctccagcta ccaaaaggga gaactctaaa agggatcaac atctcagcca atcgcggaca
    16741 aaccaccatc ataaatgcta gccttgaaaa tgcgaccctc aatacaaaca gctatatcct
    16801 ccgaattgaa ggaagtcgta tcaaaaacag taaactcaca acgcccaata tcgttaatat
    16861 ctttgataca gttcttacag atagtcagct agagtcaaca gagaatcact tccacgctga
    16921 aaatatccaa gtccatggca aggttgaact gactgccaaa gattatctca gaatcatcct
    16981 agaccagaaa gaaagccaac gaattaactg ggacatctca agcaactatg gttctatctt
    17041 ccaattcaca agagaaaagc ctgaatcaag aggtacggaa ttaagcaacc cttacaaaac
    17101 tgaaaaaacc gatgtcaagg atcaactcat tgcgagatct gatgataata ttgatctaat
    17161 atccacacca agcagacgtt gacaaaaacg cttacatttg ctactatgga aacatattta
    17221 tctaactaaa aaggaggttc taccatgaaa caagaatggt ttgaaagtaa tgattttgta
    17281 aaaacaacaa gcaagaacaa gcctgaagag cgagctcaag aggttgcaga caaggctgaa
    17341 gaaacgatag ccaatctcga tacaccaatt ggaaaaaata ctcaagtaga ggaggaagtc
    17401 tctcaagctg aagtcgaact ggaaagccaa caagaagaga aaattgaagc tcctgaagac
    17461 agtgaagcga gaacaggaat agaagaaaag aaggcatcta attctactga agaagagcca
    17521 gacctttcta aagaaacaga aaaagtcact atagctgaag agagccaaga agctcttcct
    17581 cagcaaaaag caaccacgaa agagccactt cttatcagta aatctttaga aagtccttat
    17641 atccccgacc aagctccaaa atctagggat aaatggaaag agcaagtgct tgatttttgg
    17701 tcttggctag tggaagcgat caaatctcct acaagtaagt tggaaacaag tatcacacac
    17761 agttacacag cctttctctt gctcattctg ttttctgcat cttccttttt ctttagtatc
    17821 tatcacatca aacatgctta ctatggacat atagcaagca ttaacagtcg cttccctgag
    17881 cagctagctc ctttaactct tttttctatc gtctctatcc tagtagcgac aacactcttc
    17941 ttcttttcat tcctcttggg tagtttcgtt gtgagacgat ttatccacca ggaaaaggac
    18001 tggacgctag acaaggttct ccaacaatat agtcaactct tggcaattcc aatcttcctc
    18061 actgctattg ctagtttctt tgctttcttt gatagcctac gatttacagc cctcttgtgt
    18121 gtgattagca ttggaatcat tctgcttgcc agtctccata tcattacaag accaagtcaa
    18181 gcaagtgaaa ccgactcctt ctatcaatta ttcttgtctg tccttgtgaa cggagttatt
    18241 atcctcctct tctttgtagc tgaagtggca ctgattggag attatcttcg tatcttggcc
    18301 tttctttaaa attcaatcct gtcattcttg gcaggatttt ttctatgtca aaaaagcaag
    18361 tcgattgacc tgcttctgct ttacttttct tatgctaatg ttttaaaatc ttgtactagg
    18421 atgggttgta tttctatttg attggcgtct agctggtggt aaaaagatgg gggtaacacc
    18481 tctaagaatt ttgcatagtc caagcgagct atggattcat agtcttctgg tttagagccg
    18541 tctcctgtga tttgaaccaa gtcaatctcc cactggactt ccttatccac caattgctca
    18601 atataggctg caggaacaaa cggttctctc ggtaaaactg tcttgactcc ttgagctaga
    18661 tggtaaacgc catgcacttt tccttctcca tcctgataga aggaaactct cgcaaagtag
    18721 gcatctgtct cttgaaccgc acgcacacgc gcttcaaact gagccaagcc atctgcctct
    18781 aaaaagcttt tcaagttctc acgactaaag aaaataggat taaaaattcc ttggcaaatc
    18841 gtaaaacgag ctacagggtc tgctagatag gcttgaatac ttgcttggaa ataagcttca
    18901 aaatcaaaac catctagtcc ttcaatatca gtccctgtat aagcaagcaa agcatctaaa
    18961 aatgacttga taatctgcca gtctgtcttt gttagtaggt caggaacatc gacacgataa
    19021 gccttttgac catcagcata actgacctta aagagaaatt gtgattgccc cactatcgca
    19081 cactcaatat actcgagacg attaagaggc tgacggagat agactacatc atagctatgt
    19141 gactccaaac cgtctagcaa ggccaagatc gacttggcat tcaaaatctc ttgttctcct
    19201 aaaatgctct gtttatttgg aataaaaaat gttttcgcca taactggact cctttgaaat
    19261 cgtttacata cagtgtacct tatttttctg aaagatacaa aaacaaactt tatattcttc
    19321 gaaaatctct tcaaaccgcg tcagcttcgc cttgccgtag atatatgtaa ctgacttcgt
    19381 cagtcttatc tacaacctca aagcagtgct ttgagcagcc tgcagctagc ttcctagttt
    19441 gctctttgat tttcattgag tattagattt ttgagtacaa agcatagaaa aacagcccct
    19501 aaggactgtt tgatcttata cagtagctgc ctgatccaag ctttcaccga tagcggctag
    19561 gcgctcaatc acttcagctt gtgtcaattc attttttgaa acatagcggt tacgtgggtg
    19621 aacacggcac tcgtgtgagc atccacgaag gtacttgtct tcattttctt ctgatgtcaa
    19681 gatacgacgg ttacagaagg gatttccaca gttgacataa cgttcacatg gtgttccatc
    19741 aaaccagtct ttccctacga tggttgggtt gacatggttg acatcaacgg cgatacgctc
    19801 gtcaaatacg tacatttttc catcccaaag ctcaccttga acttctgggt ctttaccgta
    19861 agttgcgatt cctccgtgca attggccgac atctttgtag ccttcacgga ccatccagcc
    19921 tgagaatttc tcacagcgaa cgccacctgt acagtaaacc acgacacgct tgtccatgaa
    19981 tttttccttg ttatcacgga cccattgtgg taactcacgg aagttgcgaa tatctgggcg
    20041 aatagctcca cggaaatgtc ctaggtcgta ctcataatcg ttacgtgtgt caaggacaac
    20101 ggtatcttta tcaagaagcg cttctttgaa ctcttttgga gacaagtaag cacctgttgt
    20161 ttcaagtggg ttgatgtcat tgtcaaagtc gttgtcttcc aaaccaaggt ggacaatttc
    20221 tttcttgtag cgaacaaaca tcttcttgaa ggcttgttca ttttcttcgt caatcttgaa
    20281 ccaaagttct tccattcctg gaaggctgtg aacgtagtcc atgtattttt gagttgtttc
    20341 atagtcacct gaaactgttc cgttaattcc ctcgtcagcg actaggatac ggcctttaag
    20401 gccgattgat ttacagaaag ccaagtggtc tgcagcaaat tgctctgcat tttcaattgg
    20461 agtataaagg tagtaaagta agacacgaat atcttttgcc ataagatttg ttctcttttc
    20521 tattcttaaa ttttctaaat tttttattta tccatactag tatacctgct tgagaaaacg
    20581 aatgcaattt atttgactgg aaattgtaga gggttctcga aatttcctga atggttaaaa
    20641 taaggacaag agaaaacatg gatatctata tccttgtgcc aaaaaaacca ctgccctccc
    20701 cagaccaacc tgaggaaagc agtgattctt attttaggag ttaggaatga atacacgaaa
    20761 tcaatttagc tgattatttt ttgtttttca agaattcatc gtattgtttt tgcatttcgt
    20821 tcaatacttt ttcgtaggca ccttcagatt tcaatttttc catcaattct ggaatagctt
    20881 tatctgggtc tacagtacca gtgttgatag ctgtatcaaa ttgttgcatt gtgttagcaa
    20941 tagctgagat ttcagatttc acattgtcag tattgaagat aaatccaagc gctggagatt
    21001 ctttagcttc tgccaattct ttcttagaat tttcgatttg ttggtctgta acgttttcgt
    21061 tgatgtaaag gatccagttg ttaccagtgt tccatccacc catgtgagtg tttcctttgt
    21121 agccatcaag aacgcgaaca cggttttctt taccttcaat tttttcccag ttcttgcctt
    21181 ctggaccgta aacaagaccg ttcaagagtt ctggattcgt attcaagagg ttcaagattt
    21241 ccattgattt ttctttgttc ttagagttgt ttgagatgac aaagttagca acttgtgttg
    21301 tttggttttt cttgatgaag ttagtaattg gtttgatttg gatatctttg ttggcaacac
    21361 gtgaaagcaa gctgttaccg tagtcagctg gtcctactgt ttcttcacga acgaaccaag
    21421 tatcttgttg aaggtcaaag gaagtatcgc ttgttgcgac gtcttttgga atgtagccag
    21481 cttcatagaa tttgtgaaga gtcttcaagt gttctttgaa acgaggcact tcgtaacggt
    21541 ttacaacttt agtagtatcg ccttcaaggt cgataacgaa tggaagaccg tttgctactg
    21601 ggtagtcaaa gttatcagat gggatgaaaa ctttaccaat agcaaatggt actacgtctg
    21661 gagctttttc tttaatttgt ttcaagactg gctcaagagt ttcgtaagaa gtaacacctg
    21721 aaatatcgat accatattta gcaaggagag ttccgttgaa ggcaaagttt tgagatgatg
    21781 caacgttggc tgcaactgga acagcgtaaa tcttaccatt tacagtatta cccttgatgt
    21841 aagctgggtc aagtgctttg taaaggtctt taccttcttt tttgtacaat tctgtcaagt
    21901 cagcgtaagc acctttttga gcatttacaa tatagttatc tgcaaaggca atatcatagt
    21961 tttcaccaga tgatgtgata actgacattt tcttaccata gtcaccccag ccaaggtatt
    22021 ggatatccaa tttggcacca actttttctt caatgatttt gttggcattt gctaacaatt
    22081 catccaagtt gtctggtttg tcaccgattt ggtacatttt gataacaggt ttgtcacctg
    22141 aatcagcagc ttttttgctg ttacctgtca aatttccaca agcagcaaga cctgcagcca
    22201 gagcgactac actagcagat gcaaaagcat attttttcca gtttttcatg ataaaaactc
    22261 ctttttttat ttttaaactt ataaacaatg taatgatctt atactcaatg aaaatcaaag
    22321 agcaaactag aaaactagcc gcaggctgct caaagcactg ctttgaggtt gtagataaga
    22381 ctgacgaagt cagttacata tatctacggc aaggcgacgt tgacgcggtt tgaatttgat
    22441 tttcgaagag tattaacttc acacaaggga agttgggaac tgagaaatgt tatttctcaa
    22501 taagcactat tctttcacac caccgatagt caaacctttt acaaagtagc gttggaaaaa
    22561 tggatacaaa atcgcgattg gaagggttgc aaccacaacc atggccatac gacctgtttc
    22621 tttcggtaga gcaactccca gttgaccagt caagccgacc gctttggcaa tgtagtccat
    22681 attttgttgg atttgcatga gcaaatattg caatggatac aagttgtcac tcttgatgta
    22741 aagaagggcg ttgaaccagt cattccagaa accaagagct gtcaaaagcg tgatggttgc
    22801 gatacctggt agtgacaatg gcaaacagat ttggaagaaa atccgggcct cactggcacc
    22861 atcgatacga gccgattcga gaatggcttc tggaatggtc ttcttgaaga aggaacgcat
    22921 caagatgata ttgaatggtg agagaagcat tggaacaatc aaggcccaaa cagtgtcacc
    22981 aagctgaagt acacgggtca ccatgatata acctggtacc aaaccagcgt tgaacaacat
    23041 actgagaagg acgaagatgg taaagaatct gcgatactta aaggttgtcc gtgaaatagc
    23101 gtaggcatag gttgttgtga taaagacatt tgtcaatgtc ccaactacgg ttacaaagac
    23161 agagatgaag agggcttgta ggattttatc cttaaactgt gccaaaaact caaaaccgtc
    23221 taagccaaat tgggatggga agaagctata gccgtattgg aggaggcttt tctcgtctgt
    23281 cactgaaata atgataacga atacaaatgg taggatacaa gagagggcaa tcaaacccga
    23341 aatgatactg aagaagatat ctgctttctt actgaaggag tgaatgccga cattatcaat
    23401 tttttctttt ttaattttct tttttgccat attctcctcc tttctagaac aaagctgagt
    23461 ttggatcgac tcgtcttgca agcaagtttg ataggataac cagaatcaaa ccaacaacgg
    23521 attggtaaag accggctgct gcagccatac cgatatctgc tgtctgagtc aaaccattaa
    23581 agacatatac gtccaaaacg ttggttacat tgtaaagctg accagcattg tgtgggattt
    23641 gatagaagag accgaagtct gcgcggaaga tatttccgac tgcaaggatg gtcaatacag
    23701 ttacaagcgg agtcaactga ggaatggtta cgttgcgaat acgttgccac ttgctagctc
    23761 cgtccactgt cgctgcttcg tagtaggttg gatcaattcc catgatcgtc gcatagtaca
    23821 tgacactgct atatccaaag cctttccaaa tacctaggaa aagtaggaga tagggccaga
    23881 tgcccaggtc agcgtagaaa ttgacttctt tgagaccaag actttccaat agatgattga
    23941 acaccccttt atcaatattt aggaaggcat ctgtaaagaa actgatgata acccaagaca
    24001 agaagtaagg gaacaacata gaagtttgaa aaatcttcac cattctctta gaacggagct
    24061 cgctgaggat aatggcaatc cctacagata caactaaacc tagaaagata aagccaagat
    24121 tgtagaggac agtatttcgt gtgataataa aggcgtctct tgaactaaat aagaatctaa
    24181 aattatcgag tccgacccat ttactattta tgatactatc tatgaaacca ttactggtca
    24241 tgtggtagtc tttgaaggca accacgttcc caaatactgg aatgtaaaag aatagaatca
    24301 accagagtgc ccctggcaaa accatcaaga gaaagatcca gttgtctctc aatgtttttg
    24361 aaaacttttt cataatttcc tcccttttta ttttgatatc catctaaaaa ttctttttta
    24421 gacttttgat aacgattaca ttattagtat actcctattt gcaggttagg ttaaactcct
    24481 aattatagaa aaaactccac aaattatgta gcagatttaa aactttatca ccactatcaa
    24541 acaaatgtcc taaatcaatt gtttatttta tctctattag cccagtgatg gcgtcactct
    24601 gttataagca tccaacaacg gggtatactg aaaaatctcc agactaggga actcagcgat
    24661 agttcctaat ctggagattt ttaatatgtt attaggcgtt tgctttcaac ttagcaataa
    24721 cctctttaag attatcaatc aactctgctg cagtatgctc agagcctttt tcatctgcca
    24781 agaacaaaac tgctttttga agttcttctt gagagttttc aaggacatcc ttatctgctg
    24841 tttctttgcc cgctgtttgt cctccatcct tagttgctga ctggctttcc ttaatggact
    24901 ctagggaagc aatggcatct ttgactgttt gcaagatatc acgtaaacct tgctctgtca
    24961 aactatcatc tgcaagagct ttcttagcct ctgccaaaac cagacgtgct gaatctgtgg
    25021 taggattcga tacacctgtc aatgatctca aaagattttc taaggtttga gtctgcttac
    25081 taatactaga ctcaaatcaa aaagcattat ataacagtga tatgaaatca actaaagaag
    25141 aaatccaaac catcaaaaca cttttaaaag attctcgtac agctaaatat cataaacgcc
    25201 ttcaaatcgt tctatttcgt ctgatgggca aatcttataa agagattata gagcttttat
    25261 agtgatttga aataagatgt gaacaactct atcaggaaag tcaaattaat ttatagaaat
    25321 attttagcag ccaaggtgta ctgttataga ttcaatacac tatacttggt
//
LOCUS       DAVHEW010000017        24730 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVHEW010000017 DAVHEW010000000
VERSION     DAVHEW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.017"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(163..1062)
                     /gene="galU"
                     /locus_tag="VVL53_000697"
     CDS             complement(163..1062)
                     /gene="galU"
                     /locus_tag="VVL53_000697"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU4182903.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(1084..2100)
                     /locus_tag="VVL53_000698"
     CDS             complement(1084..2100)
                     /locus_tag="VVL53_000698"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU4182904.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            2312..2692
                     /locus_tag="VVL53_000699"
                     /pseudo
     CDS             2312..2692
                     /locus_tag="VVL53_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(2866..3291)
                     /locus_tag="VVL53_000700"
     CDS             complement(2866..3291)
                     /locus_tag="VVL53_000700"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4182905.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(3359..4009)
                     /gene="phoU"
                     /locus_tag="VVL53_000701"
     CDS             complement(3359..4009)
                     /gene="phoU"
                     /locus_tag="VVL53_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU4182906.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(4024..4776)
                     /gene="pstB"
                     /locus_tag="VVL53_000702"
     CDS             complement(4024..4776)
                     /gene="pstB"
                     /locus_tag="VVL53_000702"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU4182907.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(4778..5593)
                     /gene="pstA"
                     /locus_tag="VVL53_000703"
     CDS             complement(4778..5593)
                     /gene="pstA"
                     /locus_tag="VVL53_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU4182908.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(5586..6449)
                     /gene="pstC"
                     /locus_tag="VVL53_000704"
     CDS             complement(5586..6449)
                     /gene="pstC"
                     /locus_tag="VVL53_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU4182909.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(6567..7442)
                     /locus_tag="VVL53_000705"
     CDS             complement(6567..7442)
                     /locus_tag="VVL53_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU4182910.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(7609..8940)
                     /locus_tag="VVL53_000706"
     CDS             complement(7609..8940)
                     /locus_tag="VVL53_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU4182911.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(8941..9648)
                     /locus_tag="VVL53_000707"
     CDS             complement(8941..9648)
                     /locus_tag="VVL53_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4182912.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(9732..10160)
                     /locus_tag="VVL53_000708"
     CDS             complement(9732..10160)
                     /locus_tag="VVL53_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4182913.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADGHYEAVTLGLDISKGQQLHYCVPKGTIWGSTVDKDDA
                     LVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            10146..10703
                     /locus_tag="VVL53_000709"
                     /pseudo
     CDS             10146..10703
                     /locus_tag="VVL53_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11018..12709)
                     /locus_tag="VVL53_000710"
     CDS             complement(11018..12709)
                     /locus_tag="VVL53_000710"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU4182914.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGIGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            12845..13291
                     /gene="argR"
                     /locus_tag="VVL53_000711"
     CDS             12845..13291
                     /gene="argR"
                     /locus_tag="VVL53_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU4182915.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            13342..15876
                     /gene="mutS"
                     /locus_tag="VVL53_000712"
     CDS             13342..15876
                     /gene="mutS"
                     /locus_tag="VVL53_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU4182916.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            16023..17717
                     /gene="patA"
                     /locus_tag="VVL53_000713"
     CDS             16023..17717
                     /gene="patA"
                     /locus_tag="VVL53_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU4182917.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(18048..18317)
                     /locus_tag="VVL53_000714"
                     /pseudo
     CDS             complement(18048..18317)
                     /locus_tag="VVL53_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18509..20275
                     /gene="patB"
                     /locus_tag="VVL53_000715"
     CDS             18509..20275
                     /gene="patB"
                     /locus_tag="VVL53_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU4182918.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIVFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            20414..21103
                     /locus_tag="VVL53_000716"
     CDS             20414..21103
                     /locus_tag="VVL53_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4182919.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            21107..21286
                     /locus_tag="VVL53_000717"
     CDS             21107..21286
                     /locus_tag="VVL53_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182920.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            21273..22622
                     /locus_tag="VVL53_000718"
     CDS             21273..22622
                     /locus_tag="VVL53_000718"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU4182921.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            22746..24206
                     /locus_tag="VVL53_000719"
     CDS             22746..24206
                     /locus_tag="VVL53_000719"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU4182922.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREEFIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            24220..24654
                     /locus_tag="VVL53_000720"
     CDS             24220..24654
                     /locus_tag="VVL53_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU4182923.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT         7344 a         4843 c         5287 g         7256 t
ORIGIN      
        1 attcggtgct tttttgtttt actatactaa tttattctac tcttatttta atgaaaaaac
       61 aaatacaagt atagcgtttt cattagtttt tattaaatag gtagattcaa gtagagaatt
      121 tacttaattt atgtgtggct aatctttata taaatgattt tgttattcct tttcagtcaa
      181 ttcttttcca agttggatga ggtaattctt caaatcatct ttgacttgtg ggtgtttgag
      241 ggcgtagtcg atggatgttt tcatgaagcc aaacttgtct ccgacatcgt aacgagcccc
      301 tttgaactca cgagcaaata cacgttgtgt tttattgagg gtgtcgattg catctgtcag
      361 ctgaatttca tttcctgcac ctggagcttg cttttcgaga atctcaaaaa tttcaggcgt
      421 gaggaggtag cgtccgataa tagcaaggtc gctaggagcg tcctctggag ctggtttttc
      481 aacaaaggtt tcaacactgt aaagaccatc ttttccttcg ccttgcggag caataacccc
      541 gtaagcagat acttcgtcat gagggactgg catgacagcg atagtagacg cgtgggtacg
      601 ctcgtagtca tccatgagtt gtttggtaag tggaacagcc ttttcgtctg tgatatccat
      661 caagtcatca ccaagcataa cgacaaaagg ttcatttccg acgaaagcct tggcttgcaa
      721 aacagcatct ccgagaccgc gtggatgagt ttggcggata aaatgcagac gcatgtcagt
      781 tgttttatca actagcttca aaagatctgt tttccctttt tctttgaggt tatattccaa
      841 ttcgaagttt gaatcaaagt ggtcctcaat agaacgtttt gatttaccag tgacaactag
      901 aatatcttca atacctgatt tgagagcttc ttccacgata aactggatag ttggtttgtc
      961 tacgattggc aacatttctt tggcaagggc cttggttgct ggtaaaaatc gagttcctag
     1021 tccagcagca gggatgactg cctttctaac ttttgatgtc ataaaaatcc tttctataga
     1081 gggttaagac cactcatttt ctgctttaaa ttcattgttc atgatgtcat aaatggcatc
     1141 tttgatattg gttccgtgat aaataacttg gtaaatagcc tgtgtaatgg gcatatagac
     1201 tccaagttct tgggctagtt cataggctgc tcgagtcgtt gaaattcctt cgattaccat
     1261 gcccatatta gcttctatat cagctaggga ttctcctcgt ccgagagcat ctccagctct
     1321 ccagttacga gagtggatgg aagttcccgt tacgatcaaa tctcccacac cagataagcc
     1381 gctataggtc aatggactgg ccccgagtgc tacccctagg cgggtgattt ctgctaaacc
     1441 tcgagcgatg atggctgcct tagcattatc accaaatcca agaccatgta aagctccagc
     1501 accgacagca ataatatttt taagagcacc agcagtttca accccgataa catccgtatt
     1561 ggtataaagt cggaagtagt gattactaaa tagcttctga acgtattgag ctgtttgtaa
     1621 atctttagaa gcagcagtta ttaaagttag gtcacgcaca atggtctctt ctgcatgact
     1681 aggccctgaa acaacgacga tatcactacg gagatgttca ggaatttctt cttcaagaat
     1741 ggttgataat cgtttatggc tatcaggttc taatcccttt gatgcgtgca tgatgataac
     1801 cttatggtcc aaggtttgtg caacttgctg ggcaacaagt cgtgtcactt ttgttgggac
     1861 aacaaacaaa atcgcatcca catctttcaa tgtttctgct aagtcggtgt aggcaatgat
     1921 attttcgtct agaacgacat ctttaaagta gtgcttatta gtatggtgtg tattaatttc
     1981 attgatttgc tcgggaagat ttccccaaat acgtacctcg tgtccattgt catttaagac
     2041 ttgtgaaagg gcggttcccc aagaaccagg ccccaagacg gcgacggttt gtttttccat
     2101 gatttcctcc ttgatagagt tctttctctt attctatcat attctagggg attttgcact
     2161 tgcattactg gggtatggtg gaaaaataca caaaaaccga gacgaaggga taaactctta
     2221 gtctttagtt aaagtgagaa gaaatctgat agatagtttg attcagtata tgcttataat
     2281 agaggtaaac aactcaggaa gttcttgtag gttgcgagag tttggcgaaa aaattaaaag
     2341 attacgtttg gctaaaaaat cagtcgttca gaattttgtg gtgatgagtc tgaattaagt
     2401 atccgtcaat taattagaat tgaaaatgga gaatccagac caatactaac aaagttaaaa
     2461 tatattgctg aacgtttggg ggttgaagat tacaagttga tgccaagtta tatagagttg
     2521 gataaggaat acctagaatt gaagtatttc ttgatgagga ctcctacata cgaagatgaa
     2581 actatcgccc aaaagaaaga gagtgttttt gataagattt ttgaagagta ttatgatagg
     2641 ctacctgagg aagaaagatt tatcatccca aattattcat atctggcact aacgaactac
     2701 acagttcaaa aattaccaga aaagctagtt gaaatactgt ccttctggtg attttgctta
     2761 atttctgatg ttcgagacat aattgaggag atttgtcttg attttttcct aaaaaagggc
     2821 agactcctcc cttggtttga tgctcgattt ttaaaataag gtgacttatt ctgttaggca
     2881 tgttttcccc tgccctctag ctctatatgg aacagggagt atcagattta tctggcagat
     2941 tgtatcaaat aaggcttgaa actgttttga ataggcgtat agacttgaga atttgagaat
     3001 atgtcgtcta gcagtagaga catattttcc ggcaatatga aggaagagac gtcgaaaacg
     3061 cttgatagtc aaggacttta cttcatcacc agctagctgc tttaaaaaga ggtagagatt
     3121 ataagccaga cagcccatca tcatacgtac ttcattctta atcatggtcg aactatctgt
     3181 cttatcccca aagaatcctg ctttcctttc tttgataaag ttttcagcat tccctcgttc
     3241 acgatagaat tgaaacaact cctcactatc tacactgctt aaattggtca aatgaacttt
     3301 tttacaacca taaaaaagag tacttaatcg tactcttctc ttttaaggat tagttgaatt
     3361 aattcaaatc cactagttct cctgtttcta ggtagactag gcgttcacaa atgttagcaa
     3421 tgtaatcagc gaagcgttcc agatgcccta tgatataaag gtattgagtt ccattgggaa
     3481 ttgaggtttc ttggtctttc ataagtccaa tgatttcctt tgataaggca taataatatt
     3541 ggtcaatctg ttcatctttt tgagcaatac taatagcttt tgaggcttgg tgcaaaggaa
     3601 aggcaaccaa taaatcggct agcatgctga gggataattt acccatttgg tgtaactgtt
     3661 cttcgtcagg ggctagttga ttttctttta gttgcaaaac agctttggca atgcctgcca
     3721 tatggtctcc catacgttca aggtctgaac aagaagacat gatgctaatc acaaatcgaa
     3781 ggtcagacac ttgtggctgc tgcaaggcca acaaacgggc acaggtcaat tcgatagcgc
     3841 tttgaccttg gttgatagca tgatccttat tgataattag ctctgccatc tccttgtctt
     3901 tggaggctaa ggccagtaag gcttttgaag ctgtttcaag gacaagttgc cctagtccta
     3961 aaaaggattg ttctaattca tgcaattcta agtcaaattg atttctcata ggtttttcct
     4021 tccttatccg aaccgtcctg aaatatagtc ttctgtgcgc tgatcttttg gattggtaaa
     4081 cacgtcaacg gtatctccaa attcgcaaat ttctcctgtt aagaaaaaag cagttttatc
     4141 tgaaatacgt gaagcttgtt gcatgttatg ggtaacaatg ataatcgtat aatccttttt
     4201 tagttgctga atgaggtctt caatttttaa agtggagata gggtctaagg ctgaagtcgg
     4261 ctcatccatt aacagaatat caggttctac tgctaaagct cgcgcaatgc aaaggcgttg
     4321 ctgctgaccg ccagataagg acatggcact ctttttaaga tcatctttga cttcttccca
     4381 aatggctgcc ccttttaaag atttctccac taaggcatct aattgttttt tgtctcgaat
     4441 accatgtgtc cttgggccat aagccacgtt atcatagata gacatggcaa agggattagg
     4501 ctgttgaaaa accatcccta cacgcttacg tagctgatta aggttgaatt tgctactata
     4561 aatatcttgc tcatctaaga ggacttggcc ttcaatatgg caagaaggaa ccaaatcgtt
     4621 catccggtta agggttttta gaaaagttga tttgccacaa ccagatgggc ctatcaaggc
     4681 agtaatctgt ctttctggta attgaatcga aatatttttt aaggcttgaa aatccccgta
     4741 aaataagtct aggtgtctga ctgaaaatgt tcccatacta ggaagctcct ttcacaagtt
     4801 ttcgagataa taagcttgat agagtattta tcattaaaac agtaataatc aaaatcacgc
     4861 cggtagcata ggcttcattg acatgtagcc cctcacttga cagcatatac atatgtaggg
     4921 ctagagaacg gcctgaagac atgagactac ttggcgtatt ggtagaggta cctaatgtat
     4981 acatgagggc agctgtttca ccaacgatac ggccaatagc tagtatcact ccagctaaaa
     5041 tacctggcat ggcaactggt agaacaattc taaaaacagt ccgtaactta cctgccccaa
     5101 gtccataact tgcttgacgc atgctatcac taacagataa aagggcttct tctgttgagc
     5161 gaataatgac tggcaacacc atgataactg aggttaagat tcctgataac agagagtatt
     5221 gaaaacctaa gaagactaca aagaagagca tgccaaacag accaaaaaca atggaaggaa
     5281 tcccagataa ggtatctgag gccaatcgca tgattttaac acaaagggaa tctttttttg
     5341 tatattccac aagataaaaa ccagcaaaaa tccctatggg caaggctaaa agaagagcac
     5401 caaagaccag aataacggtg gaaataatcg ctggcataag ggaaatgttc tcagaagtat
     5461 aagtccaaga aaagagggat agacttagat gaggtaagcc tttgatgagg ataaaaccaa
     5521 tgattaaaaa gagagagcca aaggttaaag ctgaaaaaca ataaacgaga agttttagca
     5581 ggtatttact cataagatga ttttcctttc aagtaggcaa agtaggcatt aatcaagaga
     5641 ataaggaaaa agagaactgc tgaggttgca ataagggctt ccctatgctg acctgatgcg
     5701 taagccattt ccagaacaat attggttgtt aaggttctgg ttcctgaaaa gagtccactt
     5761 ggaataatcg gctggttgcc tgccaccaaa ataactgcca tggtttcacc tactgcgcga
     5821 ccgattccta aaataactgc tgataaaata ccagatctcg cagctggcaa gatgacacta
     5881 aaaatactcc gttcatgact agctcctaga gccaagctac cagaataata cgttttggga
     5941 actgttcgga tagcagattc tgacaaactg ataatggttg gcaaaatcat tattcctaat
     6001 agtaacgaag cggttaggac actcatgcca tttcctaaaa agcttctaat ccaaggcacc
     6061 aataattgta ggccgaaaaa accataaaca atagatggaa tggctgccat caagttgata
     6121 gctgatttta agaagccata gacgggcttt ggacaataat aaaccataaa caccgatgtc
     6181 aagatgcctg ttggcacccc aatcacaatc gctcctaagg taattaataa ggaaccaacg
     6241 atcattggta aaataccata gcttgccgga atgttcgttg gcgaccaatc actgcctaat
     6301 aaaaaacggg caaagccgta gttagctatg aaaggtaagc cattactaaa aataaagaaa
     6361 cagattagca aaatagctac aacagctact gttgcactca tgaaaaaaat tgccctaaaa
     6421 actgcttctt tgaaggcttg ttttgtcaca tcttgtcctt tctagtgaag aaagtaaggg
     6481 agatacgaca cctccctact tgccttcttt atcttattgt acgatgaaac gtctgcatct
     6541 ctttagagat ttatggagca aacattttat ttaatcttgt cccaggtggt taatttgcca
     6601 ctaaaaacgt ctgcaagttc agccatactg acttggcttg ccttattgtc attattgacc
     6661 acaacagcaa taccgtctaa agcaatagca tcatgggtga gactcttacc ttcttcagga
     6721 gttaattccc tagaaaccat accaatatca gcggttttct ccttaacagc ggtaatacct
     6781 gctgaagacc cattagaggt aatatcaatc gtaacttctg gattttcttt tttataagct
     6841 tctgctaatt tttccattaa agaagatact gaagtggaac ctacaacaga caacttgcct
     6901 gataagtgtt ggcttgtata ttccgtggtt tcggttttag cttcaataaa tttattatct
     6961 gtgaccactt gttgaccttg tttggagtgg ataaagctga taaaatcttg acctagcttg
     7021 gaaagattag aagaccaaac aatgttgaag ggacgttgaa gagggtattc accatctaaa
     7081 actgtgtctc gactagcctt gacaccatca atctctaaag ccttgacaga tttcgttaaa
     7141 gatcccaagg agatgtagcc gatagcatta gcattccctt gaactgctga gagaacacct
     7201 tctgtactat tttgaatcac agctgttttg gcagtgttgt caattttttt atcaccgtct
     7261 tttttgagaa tccctgtgat ttctgtgaag gcaccccgtg tcccagagcc attttctcgt
     7321 gaaatcacct caatcgttcc tgaagctgac tgtttggaag cagctgactg attgccacag
     7381 gcaacaagcc caaatcctga taagccaatg gctgcaagag taagcatttt tttgaatttc
     7441 ataataatca cctttatctc tatgtatttt tcttgtgtag gcttactaca tttatagtct
     7501 aacaagtctt tgtaaaggtt tatccctgat tcatgtaaag attgtgtaaa gaatcaaaaa
     7561 aagccacttt tgaaaaatgg ctgctcctaa aaatagcttt aaaaattatt agtcctgtgc
     7621 gaaagattgg ttaggaagaa aaatcgtgaa gcaactgcct ctgccaagct gactcgtcac
     7681 cgtgacttgg ccacctaata attgactgag ttctttgaca atggcaaggc caagaccagt
     7741 gccaccagtt tgtctgcttc gacctttatt aactcggtaa aaacgttcaa aaatacgatc
     7801 ctgctctaat tgactaatac caatccctgt atctgataca gaaatcttaa tgccttcgtt
     7861 caccttttgg gtcttgacct caatttttcc cccttgttca gtgtaacgga tggcattgga
     7921 taaaagattg agtaagattt gggaaagtaa ttgactatct gatacgaggg tgacatcatc
     7981 tggcacctgc acctttagct gtaaatcctt cttcttgagc tgaggttgca agctttgagt
     8041 caaatcctgt acaaattctg ccaaagaaag ggtcgtccat tgtataggca tttgttgagc
     8101 cttagataag gtaagaagat gctcaacaat atgctcaaga cgcaaacttt ctttgtaaat
     8161 aatgtctaga aagtcatcct tgagcgcttc ttcttcagct gacatcccct taatggtttc
     8221 agcaaagccc ttaatcgaag taactggtgt cctcaattca tgggaggcat ttgagacaaa
     8281 ggctaaattt aacttttcat aagttctaat cgttgttaaa tcatatagca agacgagcac
     8341 agcttccaca gattgggtgg ggctaaaaac gggaactgct gtcacttcta aaatcaagtc
     8401 accctcatga aacccactta cttcttgttt taaccttgtt ttttgatcaa aggcttggtg
     8461 aactaaattc cgaatatcca tccgtttgag gtcatcaagt gaacttatgt cgccgtccac
     8521 atcgggaaaa taatgaggca gagagcgact ggataataac atctgacctt gagcggaaac
     8581 taaaaacgtc cccatggtta ggtgcgacag aagaacctcc attgtttcgg ctagatcctt
     8641 gtattgctga tcctgttggg agactttggt ttttaggcca gacacatact gagccaaaga
     8701 ctttaagtct tcttgccctt tttctaaaaa gtattcacta ctggtcaaga gaggttggtg
     8761 caaggtctca aaagcaactt cccatttcca aaggcaaaag agccagtagc cacctagtcc
     8821 caaagaaagg gctagaagaa agagaccgat gcctttactg atccaagtta atgccatccc
     8881 tgcaatcaga atgaggctaa cacttagatt gactagccaa aattgaaggt agcgtttcat
     8941 ctataactcc ttgaacttat aaccataacc ccgaatggtt cgaataaatt gaggggcttt
     9001 aggattgtct tcaatttttt ccctcaactt accaatatga acgtccacca aacgtgtttc
     9061 ctgcccaaag tcataccccc agatacgttc caaaagacgc tctctagtca gtgtcatgtt
     9121 gggatgtttc ataagataga gcaagagttc aaattctttt ggggtcaaac tcagtaactt
     9181 attcgccttg tagacttcat gacgctcagg gtatactttc aaggtcccaa atagccaaga
     9241 atcgtcagcg atattatctg aatcatctcc ttcttgttct cctttagttc gcctgaggac
     9301 agccttgaca cgcgccagca attctctagg gctaaaaggc ttggtcaggt agtcatcagc
     9361 ccctaattcc aaggccaaaa ccttatcaaa ttcatcactt ttcgcagaaa ccatcataat
     9421 tggagttttg acgcctttgg ctctcagccg cttacaaact tccatgccat ctaattgtgg
     9481 taacatgata tcaagcaaga taaaatcaaa gggttctgtt tctgccaaag ctaaggcctt
     9541 ccgtccattt gtcaccaatt gagtagaaaa gccttcctta cttaaatggt agtcaagcaa
     9601 tttcagaatg tgttcttcat catccactaa taagacttgt tttgtcatct attatctcct
     9661 attggtaaca ttataacaca attatcagaa atcctaacat tgctaaatca gattaaattt
     9721 gcctatcaag actagtatct ggtcaaacgc tcaatcatct ccttgtgctc tggataggtc
     9781 gccagtagat ctaccctttc aaataattca aaatcctcaa attcaaaacc aggagcaaca
     9841 agacaagaaa ccagagcatc atccttatca actgttgatc cccaaatagt gcccttagga
     9901 acacagtagt gaagttgttg ccctttggat atgtccaggc ctaaagtgac tgcttcgtag
     9961 tgaccatctg ctgtcaatcg gtgaaaatgt gaaggattcg tttcttctaa taagaaataa
    10021 atactggtat aaagcgccct tcccttacca gcaaggttta tagtgtctga agcttttttt
    10081 gtttgtctaa aatagccacc ttcaatatgg ggagctaact ctagagttct tatcaagtct
    10141 tctttatccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt tttacgattt
    10201 caagaactcc tctcagttct gaggacacgg taatgattga tgcgacggaa gtacaaatca
    10261 atcgccctaa aaaaagaatt agcgaatgat tctggtaaaa aaaatgccac gctatgaagg
    10321 ctcaagcgat tgtcacaagt caagggagaa ttgtttcttt ggatatcgct gtgaactatt
    10381 gtcatgatat gaagttgttc aaaatgagtc gcagaaatat cggacaagct ggtaaaatct
    10441 tggctgacag tggttatcaa gggctcatga agatatatcc tcaagcacaa actccacgta
    10501 aatccagcaa actcaagcca ctaacagttg aagataaagc ctataaccat gcgctatcca
    10561 aggagagaag caaggttgag aacatctttg ccaaagtaaa aacgtttaaa atgatttcaa
    10621 caacctatcg aaatcatcgt aaacacttcg gattacgaat gaatttgatt gctggcatta
    10681 tcaatcatga actaggattc tagttttgca ggaagtctat tatttggtta ggtgaattag
    10741 tgaagcgttt aggcaagtgt ctctggttac gacgtcatgg actctaaatc gattatattt
    10801 aggggtcatg actagtgaag cagttagcta gttcgcatat aagcggctag cgtctaacaa
    10861 ttaggaactt tagttccaat aactttaaga ttacgacgtt ttaggacata aatcgatcat
    10921 atttatgtcc taaaactagt gaagcgccta gccaaagtcc gaataggatt tggcgttagt
    10981 tacttagatt gctttgcaat caagtaactt tggcgattta catcttctct ggcgcttcta
    11041 ctccaagcaa gcgaagggct tctttgagaa cgactgcggt tgcgtagctg agggctagac
    11101 ggctgtcgcg ttctgggctt tcatccaaga tacgtgtatg tgcatagtat ttgttaaagg
    11161 attgagccag gctaattgca aatttagcaa tgatagaagg ttcaaagtta tctgccgcac
    11221 ggttgataat acgtgggaag tcttgaatga gtttaatgat ttcccagctt tcagtatcat
    11281 tcaagctata gttgccagct gtttctggtt tgaaatcggc tttgcgtaag atagattgga
    11341 tacgagcgta ggcatattga acgtaaggtc cagtttcacc ctcgaaggat accatagcct
    11401 ctaggtcgaa gtcgtatcca tttgtacggt cggttttgag gtcatagaat ttaatggctc
    11461 caatcccaac agcatgtgct acttggtctt tgttttctag ttcaggattt ttagcctcga
    11521 tttggacctt ggcacggcta acagcctctg caacagtagg ctctagcaag atgacattcc
    11581 ctttacgagt agagagtttc ttcccttctt ttgtaaccaa accaaaagga acgtgagtaa
    11641 tgtcgtcact ccagtcgtag cccatctctt gcaagacagc tttgagctgt ttaaagtggg
    11701 cagattgttc ttgaccaacg acatagatag atttagcaaa ttggtattcg tttttacggt
    11761 agagggctgc agccaagtca cgtgtgatat agagagttgc accatcagac ttcttgatga
    11821 gggctggatg ttcaattcca tatttctcaa gattcacaac ttgggcacct tctgattcaa
    11881 gaagtagtcc tttttcagaa agaatgtcta caactgcatc catcttatca ttgtagaagg
    11941 cttctccgtt atagctgtca aattcaacct tcaattcatt gtaaaggcgg ttaaattcca
    12001 ctaaactttc atcgcggaac cattgccaaa gagcgagagc ttcctcatct ccattttcaa
    12061 gtttacggaa ccattcgcgc gcttcttcat ccaagctagg gtcattttca gcttcagcgt
    12121 tgatgcggac atagagttta aggagttcat cgattggatg agcttttaca gcttcttcgt
    12181 cgccccattt tttgtaggca acaatcaaca tcccaaattg tttaccccag tctcccaaat
    12241 ggttgacctt gaccgtttga taaccgattt tttggaaaat atgtgacaag ctatctccga
    12301 taacagttga acgcaggtgg ccaatagaaa atggtttagc gatattcgga ctagacatgt
    12361 cgataacaac attttcttgt ttaccaatat tttggtcagc atagtgttct ttttcagtgg
    12421 taacagcttg caatacttga gcagaaatgg cagatttatc aaggaaaaag ttaacgtaag
    12481 gtcctgttgc gacaactttt tcaaaggctt ggctgttcat tttttcagcc agttcagccg
    12541 caatcatttg tggtgcttta cgttcgactt ttgcaagaga aaaagcaggg aaagcaatgt
    12601 ctcccatttc tgagttttta ggggtttcca gtaactttaa aatagcctct tggtccaggc
    12661 tatcaatgat gctagataat tcgctagcaa tcaattcttt tgtattcatt aagagctcct
    12721 ttttggactt ttctactatt ttatcacaat tttaaagaaa gaagaaaaaa tttttgaaat
    12781 ctcctgtttt tttggtataa tatggttata aatatagtta taaatatgca cgcaagagga
    12841 ttttatgaga aaaagagatc gtcatcagtt aataaaaaaa atgattactg aggagaaatt
    12901 aagtacacaa aaagaaattc aagatcggtt ggaggcgcac aatgtttgtg tgacgcagac
    12961 aaccttgtct cgtgatttgc gcgaaatcgg cttgaccaag gtcaagaaaa atgatatggt
    13021 gtattatgta ctagtaaatg agacagaaaa gattgatttg gtggaatttt tgtctcatca
    13081 tttagaaggt gttgcaagag cagagtttac cttggtgctt cataccaaat tgggagaagc
    13141 ctctgttttg gcaaatattg tagatgtaaa caaggatgaa tggattttag gaacagttgc
    13201 tggtgccaat accttattgg ttatttgtcg agatcagcac gttgccaaac tcatggaaga
    13261 tcgtttgcta gatttgatga aagataagta aggtcttggg agttgctctc aagacttatt
    13321 tttgaaaagg agagacagaa aatggcgata gaaaagttat cacccggcat gcaacagtat
    13381 gtggatatta aaaagcaata tccagatgct tttttgctct ttcggatggg tgatttttat
    13441 gaattatttt atgaggatgc ggtcaatgct gcgcagattc tggaaatttc cttaacgagt
    13501 cgcaacaaga atgccgacaa tccgatccct atggcgggtg ttccctatca ttctgcccaa
    13561 cagtatatcg atgtcttgat tgagcagggt tataaggtgg ctatcgcaga gcagatggaa
    13621 gatcctaaac aagcagttgg ggttgttaaa cgagaggttg ttcaggtcat tacgccaggg
    13681 acagtggtcg atagcagtaa gccggacagt cagaataatt ttttggtttc catagaccgc
    13741 gaaggcaatc aatttggcct agcttatatg gatttggtga cgggtgactt ttatgtgaca
    13801 ggtcttttgg atttcacgct ggtttgtggg gaaatccgta acctcaaggc tcgagaagtg
    13861 gtgttgggtt atgacttgtc tgaggaagaa gaacaaatcc tcagccgcca gatgaatctg
    13921 gtactctctt atgaaaaaga aagctttgaa gaccttcatt tattggattt gcgattggca
    13981 acggtggagc aaacggcatc tagtaagctg ctccagtatg ttcatcggac tcagatgagg
    14041 gaattgaacc acctcaaacc tgttatccgc tacgaaatta aggatttctt gcagatggat
    14101 tatgcgacca aggctagtct ggatttggtt gagaatgctc gctcaggtaa gaaacaaggc
    14161 agtcttttct ggcttttgga tgaaaccaaa acggctatgg ggatgcgtct cttgcgttct
    14221 tggattcatc gccccttgat tgataaggaa cgaatcgtcc aacgtcaaga agtagtgcag
    14281 gtctttctcg accatttctt tgagcgtagt gacttgacag acagtctcaa gggtgtttat
    14341 gacattgagc gcttggctag tcgtgtttct tttggcaaaa ccaatccaaa ggatctcttg
    14401 cagttggcga ctaccttgtc tagtgtgcca cggattcgtg cgattttaga agggatggag
    14461 caacctactc tagcctatct catcgcacaa ctggatgcaa tccctgagtt ggagagtttg
    14521 attagcgcag cgattgctcc tgaagctcct catgtgatta cagatggggg aattatccgg
    14581 actggatttg atgagacttt agacaagtat cgttgcgttc tcagagaagg gactagctgg
    14641 attgctgaga ttgaggctaa ggagcgagaa aactctggta tcagcacgct caagattgac
    14701 tacaataaaa aggatggcta ctattttcat gtgaccaatt cgcaactagg aaatgtgcca
    14761 gctcactttt tccgcaaggc gacgctgaaa aactcagaac gctttggaac cgaagaatta
    14821 gcccgtatcg agggagatat gcttgaggcg cgtgagaagt cagccaacct cgaatacgaa
    14881 atatttatgc gcattcgtga agaggtcggc aagtacatcc agcgtttaca agctctagcc
    14941 caaggaattg cgacggttga tgtcttacag agtctggcgg ttgtggctga aacccagcat
    15001 ttgattcgac ctgagtttgg tgacgattca caaattgata tccggaaagg gcgccatgct
    15061 gtcgttgaaa aggttatggg ggctcagacc tatattccaa atacgattca gatggcagaa
    15121 gataccagta ttcaactggt tacagggcca aacatgagtg ggaagtctac ctatatgcgt
    15181 cagttagcca tgacggcggt tatggcccag ctgggttcct atgttcctgc tgaaagcgcc
    15241 catttaccga tttttgatgc gatttttacc cgtatcggag cagcagatga cttggtttcg
    15301 ggtcagtcaa cctttatggt ggagatgatg gaggccaata atgccatttc gcatgcgacc
    15361 aagaactctc tcattctctt tgatgaattg ggacgtggaa ctgcaactta tgacgggatg
    15421 gctcttgctc agtccatcat cgaatatatc catgagcaca tcggagctaa gaccctcttt
    15481 gcgacccact accatgagtt gactagtctg gagtctagtt tacaacactt ggtcaatgtc
    15541 cacgtggcaa ctttggagca ggatgggcag gtcaccttcc ttcacaagat tgaaccggga
    15601 ccagctgata aatcctacgg tatccatgtt gccaagattg ctggcttgcc agcagacctt
    15661 ttagcaaggg cggataagat tttgactcag ctagagaatc aaggaacaga gagtcctcct
    15721 cccatgagac aaactagtgc tgtcactgaa cagatttcac tctttgatag ggcagaagag
    15781 catcctatcc tagcagaatt agctaaactg gatgtgtata atatgacacc tatgcaggtt
    15841 atgaatgtct tagtagagtt aaaacagaaa ctataaaacc aagactcact agttaatcta
    15901 gctgtatcaa ggagacttct ttgacaattc tccacttttt tgctagaata acatcacaca
    15961 aacagaatga aaaggagctg acgcattgtc gctccctttt gtctattttt taaggagaaa
    16021 gtatgctgat tcagaaaata aaaacctaca agtggcaggc cctggcttcg ctcctgatga
    16081 caggcttgat ggttgctagt tcacttctgc aaccgcgtta tctgcaggaa gtcttaggcg
    16141 ccctccttac tgggaaatat gaagctattt atagtatcgg ggcttggttg attggtgtgg
    16201 ccgtagtcgg tctagttgct ggtggactca atgttgtcct cgcagcctat attgcccaag
    16261 gagtttcatc cgaccttcgg gaggatgcct tccgtaaaat tcaaaccttt tcttatgctg
    16321 atattgaaca atttaatgcg ggaaatctag tcgttcgaat gacaaatgat atcaaccaga
    16381 ttcagaacgt tgtcatgatg accttccaaa ttcttttcag acttcccctc ttgttcatcg
    16441 gttcgtttat cctagcggtt caaaccttac cttctctgtg gtgggtgatt gttctcatgg
    16501 tagtcttgat ttttggtttg actgctgtca tgatgggaat gatggggcct cgttttgcca
    16561 agtttcaaac ccttcttgag cgcatcaatg ccattgccaa ggaaaattta cgtggcgttc
    16621 gtgtggtcaa gtcctttgtc caagaaaaag agcaatttgc taagtttaca gaggtctcag
    16681 acgagcttct tggtcaaaac ctttacattg gttatgcctt ttcagtagtg gaacccttta
    16741 tgatgttggt tggttacggg gcggtcttcc tctctatttg gctggtcgcg ggaatggttc
    16801 agtcggatcc gtctgttgtt ggttccatcg cttcttttgt taattaccta agccagatta
    16861 tctttaccat tgttatggtt ggatttttgg gaaattctgt cagccgtgcc atgatttcca
    16921 tgcgtcgtat tcgagaaatt cttgacgcag agccagctat gaccttcaag gatatcccag
    16981 atgaagagtt ggttggaagt cttagctttg aaaatgtgac ctttacctat ccaatggaca
    17041 aggaaccgat gctgaaagat gtgagcttta ctattgaacc tggtcaaatg gttggtgtag
    17101 ttggagcgac tggtgcagga aagtcaacct tggctcaatt gattccacgt ctctttgatc
    17161 cacaggacgg ggccattaaa atcggtggca aggatattcg agaagtgagt gaaggaaccc
    17221 tgcgtaaaac agtttccatc gttctccaac gtgccattct ttttagtgga acgattgcag
    17281 ataacttgag acaggggaag gggaatgcta ctctatttga aatggagcgc gcagccaata
    17341 ttgcccaggc tagtgaattc attcatcgta tggagaaaac ctttgaaagt ccagttgaag
    17401 aacggggaac caatttctct ggtggacaaa aacaaaggat gtcgattgcg cgtgggattg
    17461 tcagcaatcc acgtattctg atttttgatg attcgacctc agccttggat gccaaatcag
    17521 agcgcttggt gcaagaagct ttgaataagg acttgaaggg gacgacaacc attattattg
    17581 ctcaaaaaat tagctcggtt gtccatgcag acaagatctt ggttctaaat caaggacgat
    17641 tgattggtca aggtacgcat gcagacttgg ttgccaacaa tgccgtttac cgtgaaatct
    17701 atgaaacaca gaaatgaaag acaaactata agaaaagtca atagttttat ctaaactatt
    17761 tcttatttca atttgatgat ttggcgatga ttttagagca cggcaaaaag cccttgaaaa
    17821 agtccatttt ttcaaaggta atcctgtgtt aatttcagaa attacatcac tttttgttcg
    17881 tcaaatggca gctctttttt taggatataa aacagggttc ggataagttt ttttgcaagg
    17941 tggatgatgg ctacattgta atgttttcct tgttctaatt tagtcttaag ataggcctta
    18001 aaagcaggcg aaaagcgagg gcatgctttg gcagcttgta tgagtaccta ccgcagatga
    18061 ggggaactcc gtttgaccat tcttcctgct aaatcaatct gatctgactg ataaatagaa
    18121 gaatccagtc cagcgaaagc ttgtaattga gcaggattat caaaggcatg aatatttcga
    18181 atctcagcta aaatgaccgc ccctaaacga tccccaatcc cagtaaccgt cgtgatgacc
    18241 gagttgaact cagccatcaa gtcattgaca catgtttccg ccttgtcaat gagcctcttg
    18301 taatgtttga tgttttcatt acacgagata aaacgtctat gcgttatcaa actcattacc
    18361 aattaaaaca aaaagctgtg gttagatcct ttcggaaatt gtcaagcgat tggaggaaat
    18421 gaactaatcc acagcggctt attccaagta taccacttgg gctttggcag tagctaactg
    18481 cgctaaatat aatataagga ggagtaaaat gaagacagtt caattttttt ggcattattt
    18541 taaggtctac aagttctcat ttgtagttgt catcctgatg attgttctgg cgacttttgc
    18601 ccaagccctc tttccagtct tttctggaca agcggtgacg cagctagcca atttagttca
    18661 agcttatcaa aatggcaatc cagaacttgt atggcaaagc ctatcaggaa tcatggtcaa
    18721 tcttggcctg ctggttttgg ttctatttat ctctagtgta atatacatgt gtctcatgac
    18781 gcgcgtgatt gcagaatcga ccaacgagat gcgcaaaggc ctctttggta agcttgctca
    18841 gttgacggtt tctttctttg accgtcgaca agatggcgat atcctgtctc attttaccag
    18901 tgatttggat aatatcctcc aagcctttaa cgaaagcttg attcaggtca tgagcaatat
    18961 tgttttatac attggtctga ttcttgtcat gttttcgaga aatgtgacgc tggctctcat
    19021 caccattgcc agcaccccat tggctttcct tatgctgatt ttcatcgtga aaatggcacg
    19081 caaatacacc aacctccagc agaaagaggt agggaagctc aacgcctata tggatgagag
    19141 catctcaggc caaaaagccg tgattgtgca aggaattcaa gaggatatga tggcaggatt
    19201 tcttgaacaa aatgagcgcg tgcgcaaggc aacctttaaa ggaagaatgt tctcaggaat
    19261 tcttttccct gtcatgaatg ggatgagcct gattaataca gccatcgtca tctttgctgg
    19321 ttcggctgta cttttgaatg ataagtctat tgaaacaagt acagccctag gtttgattgt
    19381 tatgtttgca caattttcac agcagtacta ccagcctatt atccaagttg cagcgagttg
    19441 gggaagcctt cagttggcct ttactggagc tgaacgaatt caggaaatgt ttgatgcaga
    19501 ggaggaaatc cgacctgaaa aggctccaac cttcactaag ttgcaagaaa gtgttgaaat
    19561 cagtcatatc gttttttcat acttgcctga taaacctatt ttgaaagatg tcagcatttc
    19621 tgcccctaaa ggccagatga cagcagttgt tgggccgaca ggttcaggaa aaacgactat
    19681 tatgaacctc atcaatcgct tttatgatgt tgatgctggt ggtatttatt ttgatggtaa
    19741 agacattcgt ggctatgact tagatagtct tagaagcaag gtgggaattg tattgcaaga
    19801 ttcggtcttg tttagcggaa cgattagaga caatatccga tttggtgtgc cagatgctag
    19861 tcaggaaatg gttgaggtag cagcaaaagc aacccacatt cacgactata tcgaaagttt
    19921 gcctgataag tacgatactc ttattgatga tgaccagagc atcttttcaa cagggcagaa
    19981 gcaattgatt tcaatcgctc gaaccctgat gacagatcca gaagttctca ttctcgatga
    20041 agcaacttca aacgtagata cggtgacaga aagcaagatt cagcatgcca tggaggtggt
    20101 tgtagcaggt agaactagtt tcgtcattgc ccaccgcttg aaaaccattc tcaatgcaga
    20161 tcagattatt gtccttaaag atggagaagt cattgaacgt ggtaaccacc atgaactttt
    20221 gaagctaggt ggcttttatt cagaactcta tcacaatcaa tttgttttcg aataagaaag
    20281 aagttgtcct atgtgggcag ctttttcttg tccataaaaa atgtttatca cagccttaaa
    20341 aaaaacatat tagacgaaag tcattttgag tgatatgata ggactatcgt tagcattcga
    20401 aaggagaggc atcatggcta gaacggttgt aggagttgct gcaaatctat gtcccgtaga
    20461 cgcagaaggc aaaatcattc attcatctgt atcttgtaga ttcgcagaga tcattcgtca
    20521 agtcggtggt ctccctttag tcattcctgt tggtgatgag tcagttgtac gtgattatgt
    20581 ggaaatgatt gacaaactca ttttgacagg aggccaaaat gttcatcctc agttttatgg
    20641 agagaaaaag accgtcgaga gcgatgatta caatctggtc cgtgacgaat ttgaattggc
    20701 actcttgaag gaagcgcttc gtcagaataa accaattatg gcaatctgtc gcggtgtcca
    20761 acttgtcaat gttgcctttg gtggaaccct caatcaagaa atcgaaggtc actggcaagg
    20821 actacctttc gggacatctc actctattga gacagtagaa ggaagcgtgg tggctaagct
    20881 atttgggaaa gaaagtcagg tcaactcagt ccatcgtcaa agcattaaag atttggcacc
    20941 taatttccgt gtaactgcta ttgattcaag agaccaaact attgaagcga ttgagtctat
    21001 cgacgagcac cgcattatcg gtttacagtg gcatccagag tttctggtta atgaagaaga
    21061 tggcaattta gaattatttg agtatttatt gaatgaactg taacgattgg aacatctggt
    21121 cgttctttct tttatttttt caaaattttt agaatgtggc attttacgca aacgtttgaa
    21181 ttctgataaa aattggagaa attcgacaaa aaaacttgaa aaaaacgaag gtaagcgtta
    21241 tgatagaaaa gaagaaatat tggaggaaga acatgtcaca tattaaattt gattattcaa
    21301 aagttttaga caaatttgtt gcaccacatg aagtggaata catgcaatca caagtaacag
    21361 cagcagatga attaatccgt aaaggaactg gtgctggtag cgacttcttg ggatggttgg
    21421 accttcctga aaaatacgac cgcgaagaat ttgaccgcat cttgaaagct gctgagcaaa
    21481 tcaagtcaga cagcgatgtt ttggttgtga tcggtattgg tggatcttac cttggtgcaa
    21541 aagcagcaat cgacttcttg aatcaccatt ttgctaactt gcaaacaaaa gaagaacgca
    21601 aagctccaca aatcctttac gcaggaaact caatctcatc tacttacctt gctgacttgg
    21661 tagagtatgt tgcagacaaa gacttctcag taaacgtaat ttctaaatca ggtacaacaa
    21721 ctgaaccagc gattgctttc cgtgtcttta aagaactctt ggttaagaaa tacggtcaag
    21781 aagaagctaa caaacgtatc tatgcaacaa ctgaccgcca aaagggtgct gttaaggttg
    21841 aagcagacgc taacggttgg ggaacatttg ttgttccaga tgatatcggt ggacgcttct
    21901 cagtattgac agccgttggt ttgcttccaa tcgcagcatc aggagctgac ataaaagctc
    21961 ttatggaagg tgcgaatgca gctcgcaaag actacacttc agacaaaatc tctgaaaacg
    22021 aagcttacca atacgcagct gttcgtaaca tcctttatcg taaaggctat gcaactgaga
    22081 tcttggtaaa ctatgagcca tcacttcaat acttctcaga atggtggaaa caattggctg
    22141 gtgaatcaga aggaaaagac caaaaaggta tctacccaac ttcagccaac ttctcaactg
    22201 acttgcactc acttggtcaa tttatccaag aaggaactcg tatcatgttt gaaacagttg
    22261 tccgtgttga caaacctcgt aaaaacgtgc ttattcctac tttggaagaa gaccttgacg
    22321 gacttggtta ccttcaagga aaagacgttg actttgtaaa caaaaaagca actgacggtg
    22381 ttcttcttgc ccacacagat ggtgatgtac caaacatgta tgtgactctt ccagagcaag
    22441 acgctttcac tcttggttac actatctact tcttcgaatt ggcaattgcc ctttcaggtt
    22501 acttgaatgc tatcaaccca tttgaccaac caggtgttga agcttataaa cgtaacatgt
    22561 ttgcccttct tggaaaacca ggatttgaag aattgagcaa agaacttaac gcacgtctat
    22621 aatagaagaa aagagtggtt tgcccactct ttttactctc tttatccata gaaattggac
    22681 tcagccaaga cttgtgatat aatatagaaa gcaaaaaggc agacgcctag ataataggag
    22741 aaactatgtc aaaagatatc cgcgtacgtt acgcaccaag tccaacagga ctactacaca
    22801 tcggaaatgc tcgtacagca ttgtttaatt acttgtatgc gcgccatcat ggtggaacat
    22861 ttctcatccg tatcgaagat actgaccgta aacgccatgt cgaggatggt gaacgttcac
    22921 aacttgaaaa ccttcgctgg ttaggcatgg attgggatga aagtccagaa tcacatgaga
    22981 attatcgcca gtctgagcgt ttggacttgt atcaaaaata tattgaccaa ctattagctg
    23041 aaggaaaagc ctataaatct tacgttacag aagaagagtt ggcagctgaa cgcgaacgcc
    23101 aagaagtagc tggcgaaaca ccacgctaca tcaatgaata ccttggtatg agtgaagaag
    23161 aaaaagcagc ttacattgca gaacgtgaag cagcagggat catcccaact gttcgtttgg
    23221 ctgtcaatga gtcaggtatc tacaagtggc atgatatggt caaaggcgat atcgaatttg
    23281 aaggtggcaa tatcggtggt gactgggtta tccaaaagaa agacggttac ccaacttaca
    23341 actttgccgt tgttatcgat gaccacgata tgcaaatctc tcatgttatc cgtggagatg
    23401 accatattgc taatacacca aaacagctta tggtctatga agctcttggt tgggaagctc
    23461 cagagttcgg tcacatgacc ttgattatca actctgaaac tggtaagaag ttatctaaac
    23521 gtgatactaa tacacttcaa tttatcgaag attaccgaaa gaaaggttac cttccagaag
    23581 cagtctttaa ctttattgct cttcttggtt ggaacccagg tggtgaagat gagattttct
    23641 ctcgtgaaga attcattaaa ctttttgatg aaaaccgcct cagcaagtca ccagcagcct
    23701 ttgatcagaa aaaactagac tggatgagca atgattatat caagaatgca gaccttgaaa
    23761 ccatctttga aatggcaaaa ccattcttag aggaagcagg ccgtttgact gacaaggctg
    23821 aaaaattagt tgagctctat aaaccacaaa tgaaatcagt agatgagatt atcccattga
    23881 cagatctttt cttctcagat ttcccagaat tgacagaagc agagcgcgaa gtcatgacgg
    23941 gtgaaacagt tccaacagtt cttgaagcat tcaaagcaaa acttgaagcg atgacagatg
    24001 atgaatttgt gacagaaaat atcttcccac aaattaaagc agtccaaaaa gaaacaggta
    24061 ttaaagggaa aaatcttttc atgcctattc gtatcgcagt ttcaggcgaa atgcatgggc
    24121 cagaattacc agatacaatt ttcttgcttg gacgtgaaaa atcaattcag catatcgaaa
    24181 acatgctaaa agaaatctct aaataaagag gatacaataa tggacatctg ggaaaagatg
    24241 tacgaagaag cacagaaact atacaatcca catgaagtat cagactttgt ttatgccaat
    24301 catgttgtag ctgcagtaga agcaggagat ggacaaatat ttacaggatt ctgtatggag
    24361 ggaacctgtg gtgttttcca tctctgcgca gaacgggcgg ccctcttcaa tatgtaccaa
    24421 ttttcaggac aaactaaggt taagaaagta ttagcctttc gagacaaacc accttatggt
    24481 ggaagttcag ccatgccttg cggtgcttgt agagaattcc ttttagagct taatgctgaa
    24541 aataaagatg cagaattcat gatggactat aatataagaa aaacagttaa agtcgcagaa
    24601 ctaatccctt attggtgggg aaaagaacgt gcttctaagt ttaatgagcg atagaagagt
    24661 cagtataatt ctggcttttt ttactataag ttgaaaaaat aaaaaaaaat tagaaaaagt
    24721 agttgacaaa
//
LOCUS       DAVHEW010000018        24667 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVHEW010000018 DAVHEW010000000
VERSION     DAVHEW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.018"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(48..197)
                     /gene="blpO"
                     /locus_tag="VVL53_000721"
     CDS             complement(48..197)
                     /gene="blpO"
                     /locus_tag="VVL53_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU4182924.1"
                     /translation="MNTKMMEQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            479..1066
                     /locus_tag="VVL53_000722"
     CDS             479..1066
                     /locus_tag="VVL53_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU4182925.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1011..1736
                     /locus_tag="VVL53_000723"
     CDS             1011..1736
                     /locus_tag="VVL53_000723"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin ABC transporter"
                     /protein_id="HEU4182926.1"
                     /translation="MVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYILQQ
                     VMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSIIDA
                     LASTILSLFLDVSILILVEGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNHDV
                     MQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQTSL
                     KQGNKISSEYPYPMVWRSISHVE"
     gene            1726..2637
                     /locus_tag="VVL53_000724"
     CDS             1726..2637
                     /locus_tag="VVL53_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4182927.1"
                     /translation="MSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQSAKVANNRL
                     NEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIKQGDKVSLV
                     GVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQAYIFNGSI
                     LENLTLGGNHMISQEDILKACEVAEIRQDIERMPMGYQTQLSDGAGLSGGQKQRIALA
                     RALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTNKTILFVAHRLSIAERTNRVIVL
                     DQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2648..4009
                     /locus_tag="VVL53_000725"
     CDS             2648..4009
                     /locus_tag="VVL53_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU4182928.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVITSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLEKNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4066..4221
                     /gene="blpC"
                     /locus_tag="VVL53_000726"
     CDS             4066..4221
                     /gene="blpC"
                     /locus_tag="VVL53_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU4182929.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(4265..5594)
                     /locus_tag="VVL53_000727"
                     /pseudo
     CDS             complement(4265..5594)
                     /locus_tag="VVL53_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(5608..6345)
                     /locus_tag="VVL53_000728"
     CDS             complement(5608..6345)
                     /locus_tag="VVL53_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4182930.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(6350..6688)
                     /locus_tag="VVL53_000729"
     CDS             complement(6350..6688)
                     /locus_tag="VVL53_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4182931.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKKGE"
     gene            6965..7294
                     /locus_tag="VVL53_000730"
     CDS             6965..7294
                     /locus_tag="VVL53_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU4182932.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7462..8511)
                     /locus_tag="VVL53_000731"
     CDS             complement(7462..8511)
                     /locus_tag="VVL53_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4182933.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     QVLYLPYQVKRQMQD"
     gene            complement(8508..9239)
                     /locus_tag="VVL53_000732"
     CDS             complement(8508..9239)
                     /locus_tag="VVL53_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182934.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9307..9717
                     /locus_tag="VVL53_000733"
     CDS             9307..9717
                     /locus_tag="VVL53_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU4182935.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9727..10014
                     /locus_tag="VVL53_000734"
     CDS             9727..10014
                     /locus_tag="VVL53_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182936.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10176..10370
                     /locus_tag="VVL53_000735"
     CDS             10176..10370
                     /locus_tag="VVL53_000735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182937.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFEVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(10704..11840)
                     /gene="dnaJ"
                     /locus_tag="VVL53_000736"
     CDS             complement(10704..11840)
                     /gene="dnaJ"
                     /locus_tag="VVL53_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU4182938.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12242..12598)
                     /locus_tag="VVL53_000737"
     CDS             complement(12242..12598)
                     /locus_tag="VVL53_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU4182939.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTHKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12600..14423)
                     /gene="dnaK"
                     /locus_tag="VVL53_000738"
     CDS             complement(12600..14423)
                     /gene="dnaK"
                     /locus_tag="VVL53_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU4182940.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14853..15377)
                     /gene="grpE"
                     /locus_tag="VVL53_000739"
     CDS             complement(14853..15377)
                     /gene="grpE"
                     /locus_tag="VVL53_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU4182941.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15404..16438)
                     /gene="hrcA"
                     /locus_tag="VVL53_000740"
     CDS             complement(15404..16438)
                     /gene="hrcA"
                     /locus_tag="VVL53_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU4182942.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16602..17111)
                     /locus_tag="VVL53_000741"
     CDS             complement(16602..17111)
                     /locus_tag="VVL53_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU4182943.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17719..20053
                     /locus_tag="VVL53_000742"
                     /pseudo
     CDS             17719..20053
                     /locus_tag="VVL53_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            20066..21529
                     /locus_tag="VVL53_000743"
     CDS             20066..21529
                     /locus_tag="VVL53_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU4182944.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21529..23097
                     /locus_tag="VVL53_000744"
     CDS             21529..23097
                     /locus_tag="VVL53_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4182945.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     NEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIIRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            23154..23951
                     /gene="psrA"
                     /locus_tag="VVL53_000745"
     CDS             23154..23951
                     /gene="psrA"
                     /locus_tag="VVL53_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU4182946.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLTFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            23962..24571
                     /locus_tag="VVL53_000746"
                     /pseudo
     CDS             23962..24571
                     /locus_tag="VVL53_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(24519..>24667)
                     /locus_tag="VVL53_000747"
     CDS             complement(24519..>24667)
                     /locus_tag="VVL53_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4182947.1"
                     /translation="TKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVN
                     QLWK"
BASE COUNT         7946 a         4857 c         4516 g         7348 t
ORIGIN      
        1 agcaccagtt acacctattc ctatagcaaa tggtcccaat agaaatgtca aaccgttgtt
       61 gcacacccat caattgcgcc atatgcaacc cctgctgcac aactaatttt tcttccccaa
      121 tcaatatctc caccttcaac gcaagcaagc atttcattat ccataacaga aaattgttcc
      181 atcatttttg tattcatgac aaatactcct tttttctttt tttatttttg tcttgttgta
      241 actttgataa gtttagtata tcatcgtttt ttaaaatttt tcatccagat cttgaattgt
      301 catcgaaacg tcttgaatta gcttttttat ttcaagccac ctctaaatgt ttaaaaaaaa
      361 taatttctaa tcactttttt accattcagg aagttttaat gactattcaa gatttcataa
      421 aatatgaact tagttttatg acataataga cctatccact atatgaaagg aattgccaat
      481 gacttcttat aaacgtacat ttgttcctca aatagatgcg agagactgtg gtgtcgctgc
      541 cttagcctcg attgctaaat tctatggttc agatttttct ctagctcact tgagagaact
      601 tgcaaagacc aataaagaag ggacgactgc tcttggcatt gtaaaagccg ctgatgaaat
      661 gggctttgaa acaagacctg ttcaagcaga taaaacgctc tttgacatga gtgatgtccc
      721 ctatccattt atcgttcacg ttaacaaaga aggaaaactc caacattact atgttgtcta
      781 tcaaacaaag aaagactatc tgattattgg tgatcctgac ccttctgtaa aaatcactaa
      841 aatgtcaaaa gaacgctttt tctatgaatg gactggagta gctatttttc tagctaccaa
      901 acccagctat caaccccata aagataaaaa gaatggtcta ctaagcaagc ttccttcctc
      961 tgattttcaa acaaaaatct ctcattgctt acattgttct ctcaagctta ttggtcacta
     1021 ttatcaatat aggtggttct tactatctcc aaggaatctt ggatgaatac attccaaatc
     1081 agatgaaatc aactttagga atcatctcag ttggtctggt tatcacctat atcctccaac
     1141 aagtcatgag cttctccaga gattatctcc taaccgttct gagtcagaga ttaagtattg
     1201 atgtgatttt atcctatatt cgccatattt ttgaacttcc catgtctttc tttgcgacac
     1261 gtcgtacagg agaaatcatt tcacggttca cagatgctaa ctctattata gatgccttgg
     1321 cttctaccat tctttctctt tttctggatg tttctattct gattcttgta gagggagtct
     1381 tgctggcaca aaaccctaat ctcttccttc tttctcttat ttccattcct atatacatgt
     1441 tcatcatctt ttcttttatg aagcctttcg aaaaaatgaa ccatgatgtc atgcaaagta
     1501 attctatggt tagctctgcc attatcgaag atatcaacgg gattgaaact ataaagtcgc
     1561 tcacgagtga agaaaatcgc tatcaaaata tagacagcga atttgtagat tatttggaaa
     1621 aatcctttaa gctcagtaaa tattctattt tacaaacgag tttaaagcag gggaacaaaa
     1681 ttagttctga atatccttat cctatggttt ggcgctcaat tagtcatgtc gagtaaaatt
     1741 tctatcggtc agctgattac ctttaacaca cttctttctt actttacaac tcctatggaa
     1801 aatattatca acctccaaac caaactccaa tctgcgaagg tcgctaataa ccgtttgaac
     1861 gaagtctatc tagtcgaatc tgaatttcaa gttcaagaaa accctgttca ttcacatttt
     1921 ttgatgggcg atattgaatt tgatgacctt tcttataagt atggttttgg acgagatacc
     1981 ttaacagata ttaatctcac gattaaacaa ggagataagg ttagcctagt tggagttagt
     2041 ggttctggta aaacaacttt agccaaaatg attgtcaatt tctttgaacc ctacaaaggg
     2101 catatttcca tcaatcatca ggatattaaa aacattgata aaaaagtctt gcgccgtcat
     2161 attaattacc taccccaaca agcctatatc tttaatggct ctattttgga aaacttaacc
     2221 ttgggcggta atcatatgat tagtcaagaa gatattctaa aagcttgtga agtagctgaa
     2281 atccgtcaag acattgaaag aatgcctatg ggctatcaaa ctcagctctc tgatggagct
     2341 ggtctatcag gaggacagaa gcaacgaatc gctctcgctc gtgctctttt aactaaatct
     2401 cctgttttaa tactagatga agctactagc ggtcttgatg tcttgactga gaaaaaggtt
     2461 atagataatc ttatgtccct aactaataaa accattctct ttgtagccca tcgtctcagt
     2521 atagccgaac gaactaaccg tgtcattgtt cttgaccagg ggaaaatcat tgaagttggt
     2581 agtcaccaag agttaatgca agcgcaagga ttctaccatc atctattcaa taaataagga
     2641 gaatgtcatg aatcctaatc tttttagaag tgtcgagttt tatcagagac gttaccataa
     2701 ctatgcgaca gtattaatca tacctctgtc attactgttt actttcatct tgattttctc
     2761 ccttgttgcc acaaaagaaa ttactgttac atcgcaaggg gaaatcaccc ctacaagtgt
     2821 cattacatct attcagtcga ccagtgataa tcctatccta gctaatcatt tagtggcaaa
     2881 tcaagtagtt gagaaaggtg acttactcat caaatactct gaaacaatgg aagagagtca
     2941 gaaaactgcc ttagcaactc aattacaaag acttgagaag caaaaagaag gacttggaat
     3001 tttgaaacaa agcttagaaa aagcgactga tcttttttct ggcgaggatg agtttggcta
     3061 ccataatacc tttatgaatt ttactaaaca atcccatgat attgaactcg gtatctcaaa
     3121 gactaacact gaagtttcaa atcaagccaa tcttgccaat agcagttcat cagccatcga
     3181 acaagaaatt acaaaagttc aacaacaaat tggagaatat caagagctga gagatgctat
     3241 cataaataac agagctcgct taccaactgg caatccgcac cagtcaattt taaatcgtta
     3301 tcttatagcc tctcaagaac aaacacaagg aacggcagag gagccatttt tatctcaaat
     3361 taatcaaagt attgcaggtc ttgaatcatc tatcgcaagc ctcaaaattc agcaagctgg
     3421 tatcggaagt gtagcaactt atgataatag tttagcaacc aaaattgaag tactccgcac
     3481 tcaattttta caaacagctt ctcagcaaca actaaccgtg gagaatcaat taacagaatt
     3541 aaaagtacaa ctagatcaag cgacacagcg cttagaaaag aataccttaa ccgctccaag
     3601 taaaggtatc gttcatctga acagcgaatt tgaaggtaaa aatagaattc caactggtac
     3661 agaaattgct caaatattcc ctgtcatcac agatacaaga gaagtactaa tcacttacta
     3721 cgtatcatct gactatctcc ccttactaga taaaggacaa actgtaagat taaaactgga
     3781 gaagattgga aatcacggca ccaccatcat cggccaactt cagagtattg atcaaactcc
     3841 taccagaaca gagcaaggca atctctttaa attaaccgct cttgcaaaac tatctaacga
     3901 ggatagtaaa ctcatccaat atggcttaca aggtcgcgtc actagtgtaa ctacaaagaa
     3961 aacatatttt gattatttca aagataaaat tttaacccat tctgattaat tttcagataa
     4021 cactctataa ctatttatta tcttatcaaa aaggagaatc ataacatgga taagaaacaa
     4081 aacctaactt catttcaaga actaacaact accgaactca accaaattat aggtggagga
     4141 tggtgggaag atttcttata tagatttaat ataattgaac aaaaaaatac aaaaggattt
     4201 catcagccaa tacaactata aaaaaacaag accaagagaa cgcactctcg gtcttatttt
     4261 ttcttcattc tatatgtatt acagtaagta cctgacgaaa aacgtgatct tggcaggtgg
     4321 tattgagact ggtattggga tgactttcca caattttcat aacggtatag agaccaactc
     4381 ctctctcctc ccctttagaa cttgctccaa aggagaagat ttcagaaata tcgatgccct
     4441 cttctttgat ggaattttca atgataaagg tctcctgtgc tccatttttt aaaaaggcaa
     4501 ttgaaacatg aggttgacag gcttctacac tagcttcaat agcgttgtca caaaggatag
     4561 acacaacggt tagaaaatca agtagactca tcccctcaac ctgaatctcc tcaggaactt
     4621 caacattaaa gacaatgttc ttttctctag cttttataaa ttttccagct aggagacttt
     4681 tgagggcttt atcacgaata ttcaccaatc gaccaaggtc atatttattg tcctgcaatt
     4741 tttcactaga atcctttaag accgagtcgt agatctcttt tatctgctcc atatcctcct
     4801 cttcaatgcc cagacgtaag ctagtcaaga ggttggtgta gtcatggcga aaactccgta
     4861 cttccttata aagctcctct atatgccgac tatagcgttc catctctctg tagcgcaagt
     4921 cctgctcttg gttcagtctc tcatggagtt tgtccttcaa ataggtatcc aatttcttga
     4981 taatccccat aaaaaagagc aggtaaaaga ctaggatgag atggcgaaca gtcgttgatt
     5041 gaatcccctg ttcatattca aagtaagaca gattttgtat caccaaatag taagccccca
     5101 ttatccagtt aatctgagtc agggactttt gaaaatcttt atcgagaatc ccctttctca
     5161 agctagtgaa atcatagtcc aaccatttca aaaaagctag agaaatgaag aaattgaaaa
     5221 ttattataca taaccaaata aatgagtagt catcatatac ttgcccttgt cccaaaaatg
     5281 gaagcacaaa ataggaaaca cctctataaa agagattcac taatatcatt ggaaagagac
     5341 cataaaagag aaggagtttt ttaggaagtt ctcttaaata agcctatgcc gtacaagggt
     5401 tccataaaat aagataggta aacatttcct accatatagc caagcattcc aaaaacaacg
     5461 gccaacagta tcttaaaaag aaaggcctta aaaatcctct caaaagtgag actaattcca
     5521 tctaccttaa agaatatgac aatttttaat ccgtgagtaa caagtgtata caacaatatc
     5581 caagcaacat tcataaactc tcctagctca gtgtaagtta ttaatagcct cagacacttc
     5641 cctgacctta taacgcgcga ttagacaact tccaccattg ggaaagaaaa gcagtttttc
     5701 tttcttatct aaatgcacca cattggcagg attgatgaga aaagagcgat ggcactgcaa
     5761 gagacgaggc tcctgcttga gaacctcctc tagactcgct gtaaattcca gcctgtctgt
     5821 cttggtatag agaataacac gatggggtct tgtggacgtt tcgagatagt aaacctcttt
     5881 aaaaggatac tggaattgag caaattttga tttaaagtaa aagcaatctt ccgctagact
     5941 tttactgtct tgaccattgg catagaggag ggctgtctcg atacgagatt caaactcctc
     6001 tgccgaaagg gccttatcaa tatagtccag agcagacact tggtagcgaa aggacagggg
     6061 cataaactcc gagtgagtcg tcacaaagac aatcagggca tacggatccc gatcccgaat
     6121 ctttctagcc acttccagtc ccttcatctc ttcatttcga atctcaatat ccaaaaagaa
     6181 tagctgatgg gcccccttct catgcacctc tgccagcagt tggtccggct tgccaaatac
     6241 ctcaaaagag ctaggaatga tatgatgtgc tttcaaaagt ttctcaatcg tcgtttcaat
     6301 tctagtctgt tgggaaaaat catcttctaa aacaaatatt ctcatcttct tactctccct
     6361 ttttcaacca tttttgacga atttctctat agcgacgtct ggcatacttg acagcgtatt
     6421 gaccttcttc tccgagaaaa aggattcttt cttgaaaatc aatcgatttt aatttatcaa
     6481 gattaaccaa acaatttcga tgacatctca tcaaggtttc cccacattgg ttctcaagat
     6541 tacttaaatt ttgaagcata ttaaaactag cttcttctgt aacaatctgt acggtatggg
     6601 ctttagttgg atgtgtttgg atatagtaga tatcgtcgat gtttacttta tagactgttt
     6661 tctgcgtctg aataatcata tatttcattg gattctcctc ctaagaaaag aataacagaa
     6721 aaggtctagc ccaagcacac ccaatcctaa acagtctttg aaaactccta aaaggattat
     6781 tttctacaac atcatgatga gcttacaact attttatcat aaaattcact cagcgcaatt
     6841 caagacattt caatgacaat taaagatttg aataaaaaaa aaagaattaa aagtgataaa
     6901 ctaacagtat caaaacacag ttgccagaag taagactggc acccagatta aaggaggcat
     6961 tcccatgaca gatacagacc ccatcaaaag agctcatact ttgattactg acttaaacaa
     7021 agcctaccaa gcatgcaaac aggcaagcgc tgacgacgtc cgctttcagg agcaattaaa
     7081 ctctattctt ggttttctag ccaaggctga aacagtggat aatcgattct tgattgaact
     7141 agaaaaattt taccagactt ccagtcttct catgggactc agcgctcttg atccagatgc
     7201 tccaactcgc gccgcttggc gggcctatga ccgcttccac tttgaccaag tcaagaccaa
     7261 gttaatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaagc tgtacttgag
     7321 tacggtaaga cgacgctgac gtggtttgaa gagattttcg aagagtataa gtctctacgg
     7381 accaaccatt atcctgtagt aacaaacaag aagctgagac agactgaact caacttcttt
     7441 tttagtgtcg tatcagcaat gttagtcctg catctgacgt tttacctgat aaggcaaata
     7501 caagacttgt aaaaccaaac cagctcccag taaggctaga agggctagtt tttcttgaaa
     7561 ggtaatcaac ccaacaacta attccacaag taaaacaaaa ctggtcaatc ctcgaactac
     7621 ctcctgtaag cctttttcct tttgcccctt gtccagcgga aagagttggg tcaaatactg
     7681 gtagtcaaag gcatgataga gggccagcaa ctggaagagc aagaggtagt taaagagaac
     7741 taccactgct gtcgcaatcc aagcttgctc gataaaaacc tgcgccagta cggaaagtaa
     7801 gagaagacga agactgagag caaagaggtc gccatttcgc agataagaac gcagatagag
     7861 attttgccaa atcttcccag gcaccttctg aacagccttt aagataaagt ccagataggc
     7921 acgacgctta acgctgtttg aaattccctt gacctgcgta aagagggcaa agaaacgaag
     7981 caagacttgc ttacgcttgc tttcttgaga aataacatag tcccaatcca gtccagtttc
     8041 agtgaaaaat ttgctggcct tttgacgaaa gtggaaatat tttcctaccc ccaataaaag
     8101 cacatagagc agaaaaactg gcaagccata acccattgct aaaaataaag gcgcaaataa
     8161 cagcaagaaa agggtctgta caaagagcca aaagactagg gaaatgccag tttgacgctt
     8221 gagatggagc ttgatttcct cttctccaac taagagaaag agcttgtctg gagcctccat
     8281 ataggtggca attcctcccc aaagtaaaag taaaacagag gtaattccta caaacaaaag
     8341 gataggccaa tgattttcag gaaaatgttg taagagttga ctgtactggt aggctagaaa
     8401 gcccaacagt acaagcagga acaagacaaa gtggtcattg agaacatagc gcagataacc
     8461 aagacactcc ttacgaaagg cctgctttct ctttaaaaac aagtctttca taggtcctcc
     8521 tcttttgtca gagccaagta aatatcattc aaactagcct caggcatatc aaaggcttcg
     8581 cgtagttgca ggagattgcc tttggcacgc acctctccct tgtgaagaat gacaaaggca
     8641 tcacacatct tctccgccga atccagcacg tgggtactca tgagaataga cttgcccttt
     8701 tgcttctcca cttccaaaag ctgaatcaaa tcagaaatag ccagcggatc aagaccaagg
     8761 aaaggctcat ccacgatgaa aagacttgga tccgccacaa aagcacagat aatcatgacc
     8821 ttctgcttca tcccttttga aaaatgaaca gggaaccagt ctaatttctg ttccaaacgg
     8881 aacattttta acaagggctc tactcgttcg aaagccactt tttgctcaat accgtaagcc
     8941 atagcaaccg tttcgatatg ctctctgagg gtcaattcct catacagact aggcgtctca
     9001 ggaatgtagc caatctgctt gcggtagcta gtcgcatctc cttgcagagt caggccattg
     9061 atattgatgg agccactata aggtgccaac agaccgataa tctcattgat cgtcgttgat
     9121 ttcccagcac cattgagacc aatcaaaccg accaactgcc cactttcaac agtaaaggac
     9181 acatctttca aaacaggaac atgaacatag ccacctgtca ggtttttaat ttctaacata
     9241 ttttctccaa atctggtata atgtagctat attatatcaa aattcagtac agtagaggta
     9301 gattttatgt cagattgcat tttttgtaaa atcatcgcag gggaaattcc tgcttcaaaa
     9361 gtatatgaag atgagcaggt ccttgccttt cttgatatct ctcaagtaac actaggacac
     9421 accttggtcg tgccaaaaga acactatcgc aatcttttgg agatggatgc tacgagcgcc
     9481 agccaactct ttgcccaagt accaaaagta gctcaaaaag tcatgaaagt cactaaggct
     9541 gctggtatga atatcatttc caactgtgaa gaagtcgctg gtcaaacagt ttttcatact
     9601 cacgttcacc ttgtacctcg ctacagtgct gacgatgacc tcaagattga ttttatcgcc
     9661 cacgaaccag actttgacaa acttgctcaa gtcgctgaaa ccatcaaaaa cgcttaagga
     9721 gttgctatga aattatccaa cctactgcta tttgcaggag ctgcagccgg aagttatctg
     9781 gtcacaaaaa atcgccaaac catcacagat gaagtcttga ataccactga ccgcgttcaa
     9841 gctatcaagg acgatgtgga tattatccaa aacagcctgc aaatcattga ccagcaaaaa
     9901 gaacttatca aggaatacca agaagacttg acttacaagt ttaaggtctt ggaaaaggat
     9961 atccagacta gactagctgt gataaaagaa atgcagggaa ctgaagataa gtaaaaagag
    10021 ccccccggct cttttttaaa acgtaaacaa gttatagtga attgaattta gaatagcaca
    10081 tcatagtttc taaagcattg ttagagatta ctttaaaatc ctcttatcaa tttgttcata
    10141 ttctatttca atctactata tatatcccat caactatgca tcataattta ggtaactcat
    10201 actcaataaa aatcaaaaag caaactagaa agctaggcac agactgctca aaacaccgtt
    10261 ttgaggttgt ggatagaact gacgaagtca gtaaccatac ccatgacaag ccgacgctga
    10321 cgtggtttga agaaattttt gaagagtatc attcaccatt tcacaactaa atcgaagact
    10381 ttctgccgta atgaaaacac ctgaaacagc ttggtttcgg ctgtccggaa actttaagac
    10441 ttaggttcaa agtttaggta tggaacttcg aaggaggtcg ctaccgtccg tcattactta
    10501 ggaaagtctt aaaaaatcta taaagtaaaa agagcccgac agctcttttt gtttatactc
    10561 aatgaaaatc aaaaagcaaa ctaggaaact agccacgggc tgctcaaaac actgttttga
    10621 ggttgtagat acgactgacg aagtcagtca catatatacg gcaaggcaac gctgacgtgg
    10681 tttgaagaga ttttcgaaga gtattattct ccatcaaagg catctttaat atggtcaaag
    10741 aagccttttt tctttggatt tactttcaag tcaccagcag ccgcgaattc tttcaaggct
    10801 actttttggc ggtcgttcaa gcctgtcggt gttacgacat taacagtaac gtattggtca
    10861 ccaactgcac cgccacgaag gctcggtgcc cccttactac gtaggcggaa cttcttacca
    10921 gtctgagttc cctctggaat aaccaattca acatcaccgt gaacagttgg aatatctact
    10981 gtatcaccaa gagccgcttg gacaaagttg aggttgagat tgtagaagat agtcgttcct
    11041 tcacgttcaa acttgtcgct agcttccaca gaaactacta catacaagtc accataaggt
    11101 ccaccgttaa agcctgcttc accttgacca gcgaggcgaa tttgttgacc tgtttccaca
    11161 ccagcaggga ttttcacatg tacgctatga gcttgtttct catgacctgt cccatgacag
    11221 gttgtacatg gatatttgat ttcttttcct cgaccgtgac agacatcaca ggttacttgg
    11281 cgacgcatca taccaagagg agtctgcgta tcgacgttaa tgacaccagc gccatgacag
    11341 cgtccacaag tgactggact tgtccctggc ttagcaccag atccattaca tgtacgacag
    11401 ccagcttcac gatgatactt aacttccttc tcagttccga agatagcttc ttcaaaggtc
    11461 aaattgacac gatactggag atcatctcct tggcgaggag cgtttggatt gcgcgaagaa
    11521 ccgcctccgc cgaagaaact tgagaaaata tcctcaaaac caccgaaacc acctgcccca
    11581 ttgaaaccgc cgaaaccacc agctccacca aaaccaccat tggcgcctgc agcaccatac
    11641 tggtcatagg cagcacgttt ttggtcgtca ctcaaagtct cataggcttc ttgaacttcc
    11701 ttgtacttgt cctcagcacc aggctccttg ttgatatctg ggtgatattt tttggaaagc
    11761 ttacgataag cctttttgat ttcgtctgcc gaagcgtttt tggatacccc cagacgatca
    11821 taaaattcag tattgttcat aattcaagat acaaagggcg tcaaacggac acagccatct
    11881 aggagttttg acgacggacg cttacgtcct agaaaaaact atcttttggg cacagtccgt
    11941 agcccgtgtt cagttacgaa cacccttatt cctttctatt gatgttctga atcgaaccac
    12001 catttaggtc gggttcaatt cctttctttt atatttagta agaaattctg aaacccgtgt
    12061 tcagttacga acactctttg ttcctttcta ttgatgagac aaaacccaaa caaatgagat
    12121 tccgtccctt tttatcgaaa aacagaggct gggttgcaac ctctgcaaat aagtaaatta
    12181 actttatttt gattttaaat atcctataag tacagaaata tttatatatt tttgtctctt
    12241 cttagtattt gcctatataa ttccaattga tcataagaaa tacaactact gtccaaatca
    12301 taccaattat gaacggtatt acaaatttat gtgtttgtat ttctaatgat atcatagctg
    12361 ataccatcgc cgatacaaat gcagacaaca atactgaggt aacaaaattc cttaccgctt
    12421 catcattttt gatacaacta tcatgttcag ctaatataat ttctctatat ctagctaaat
    12481 attctattgg aatattatca ataaaatctt ttgatgttct atagttatca gcttgattac
    12541 aaaaatcatt ataaactttt cgtgtatttt ttagatactc ttcatccaat acactcatct
    12601 tacttttccg taaactctcc gtctacgacg tcatcgcctg cgtttcctgt tgcttgtgcg
    12661 ccttctgctc cttcttgagc ttgttgcgct gctgcggctt gttcgtagag tttaacagca
    12721 agtccttgag ctttttcgtt caatgcttca agttttgctt tcatgtcgtc caagttgttg
    12781 tcttcttgag ctttcttaag gtcatcaagg gcagcttggg cagcgtcacg ttctgcgtcg
    12841 aagcctttac cttcagtttc cttgattgtc ttttcagtcg caaagattgc ttggtctact
    12901 tcattacgaa ggtctacttc ttctttacgt ttcttatcgg cttcagcgtt tgcttctgca
    12961 tctttcatca tgcggtcgat ttcttcgtca gtcaaacctg agttcgattg gatgacaata
    13021 gtttgttctt tttgagttcc aaggtctttg gccttaacag acacgatacc gttcttgtcg
    13081 atgtcaaatg ttacttcgat ttgaggaatt ccacgaggtg cagctgggat atcagtcaat
    13141 tggaagcgtc caagagtctt gttatctgct gccattgggc gttcaccttg aagaacgtgg
    13201 atatcaacgg ctggttggtt gtctgctgct gttgagaaga cttgtgattt agatgttggg
    13261 attgtagtgt tgcgatcgat aagttttgta aatactccac ccattgtttc gataccaagt
    13321 gacaatggcg ttacatcaag aaggacaaca tccttgacat caccagtaat cacaccacct
    13381 tggatagccg cacccatagc aactacttca tcagggttta ctgatttgtt tggttcttta
    13441 ccagtttcag ctttaacagc ttcaacaacg gcagggatac gagttgaacc accaacaagg
    13501 ataacttcgt cgatttctga caagctcaaa cctgcatctg aaagggcttg acgaactgga
    13561 acttttgtac gttcaacaag gtcacgagtc aaatcatcaa atttcgcacg agtcaaagtc
    13621 atttccaagt gaagaggtcc agcctcacct gcagtgataa atggcaagct gatttgtgtt
    13681 gaagttacac cagaaaggtc tttcttcgct ttttcagccg catctttcaa acgttgcatt
    13741 gccatcttgt cagtagacaa gtcgataccg ttttctttct tgaattctgc taccaagtgg
    13801 tcaatgattt tttggtcaaa gtcgtcacca ccaagtttgt tgtcccctgc agttgacaat
    13861 acgtcgaaga caccgtcacc caattcaagg atagagacgt cgaatgtacc accaccaagg
    13921 tcaaatacca agattttttc ttctttgtca gtcttgtcca aaccataagc aagagctgct
    13981 gcagttggtt cgttaacaat acgttctact tcaagaccag caattttacc agcgtctttt
    14041 gttgcttgac gttgagcgtc gttgaagtaa gccggaactg tgataacagc tttggttact
    14101 ttctcaccaa ggtagtcttc agcgtagcct ttcaagtatt gaaggatcat agctgagatt
    14161 tcttgtggag tgtattcttt tccatttgca gaaacttttt cagaagttcc catcttagat
    14221 ttgatagaga taactgtatc tgggtttgtg actgcttgac gttttgcagc atcaccaacg
    14281 atgatttctc cgtttttgaa tgagactaca gatggagttg tgcggtttcc ttctgggttt
    14341 gcgatgattt tgctttcagt tccttcaaga actgcaactg ctgagtttgt tgtacctaag
    14401 tcaataccga taattttaga catgtgtttt tctccttgtt agttaatttt ctatttttct
    14461 ttttgatact cttcttaagt ggcgacgccg tcagagcttg acttcgtcaa tctctttgac
    14521 taaactttga gcctaaggtc tcaaaatttg cgcaaatagc gccacggcga agagtatcgt
    14581 ctttggttcg cttcgctcac tattacaaag ccaaacatat tttatctttc ttcatagttt
    14641 attgtcgttt cggacaagtt ttctattatg taaattgcga cacgaggagt cgaaatcgat
    14701 tttatttcga cgacgagtta gtaaggaagc taggcaaacg ccatagcgat tgccgttttc
    14761 tgacgagttg ctttagctac cgtcagaatt gccgaatcga acacttcgtc aatctcctat
    14821 ttttactgcg agctttttac gagctttgta tcttagttat acaccactac cattgctggg
    14881 cgtaggatgc ggtcatggag tttgtagcct ttttgaaaga cttgggcgat ggtatctact
    14941 gggtgttcat cgtctgctgg gagagtttgg atggccatat ggtagttatg gtcaaattcg
    15001 ccatctgctg cgatttcttc aattccttct tctttcaaag cgtgaatcaa gctttcttgc
    15061 accatcccca agcccttctt cacatcatct gtcaaacctt caactgcaag tgcacgctca
    15121 aggttgtcaa gagatggtaa gattgctttt gccaagtcct ggctacgata acgttgcaag
    15181 ttttgacgtt cttcattggc acggcgttgg atattttgca tttctgcatg agcgcgaaga
    15241 tatttgtttt cgaactcatc tgcacgttca tttgccaagt ccaactcaga cttttcagga
    15301 gttgtttctt cagctgtttc cacaacttcc tcttcttgaa cttcttctac ttcttcattt
    15361 tttatatctt gggccatgtt cgcctccttt aatgatttca atcttaatgt acttcgtaat
    15421 gattactgct gaggtagcgg taaaaatctg tcaacttcat ggtcaaaaca cggttgacca
    15481 cattgacttg attgattagc tgttggtaat ccagattaac tggaccgata atggctagaa
    15541 ttccaactcc ccgataagga atgaggaact tactactgat taccgctagg tcagctaaac
    15601 aggactcttg accgtctgca acacgaacat tttgcatctg atcctcacgc aacccctcac
    15661 gaatctccaa ggccaccttt tgcggttggt caaagaactg ataggctgct agattggcaa
    15721 aattcaagag atggaccttg cccgccatca caatgttttc gttgaacatt tccttaaaga
    15781 tgtgttcaaa gagatcgatg acattatccg ttgttgtaaa gtaacgctgg ataatctgcg
    15841 gaatctccgt ccgaatcttg tagtgaatat ctaaaacggt gtgaccgagg aaacgttcct
    15901 gaatgatgct cttcagtttc agcaaatcct cctgcaagaa gttccttgga atcagaaact
    15961 gactagtaac cgttcgcgac tcgtctaggg taaataccgc caaggctgta tgttgcccca
    16021 aaacaacgat atcaaaggct gtcaaacgtt gcctgctcgg ctcaacatcc agtgccacta
    16081 ccgtacagcc actcaggtct gttagtaagt tagcagcctc ttgcagaata tcctccaatt
    16141 tgaagaattc ctgatcaaag gctttgacaa tctcatatac ctcattttca gccagccggt
    16201 caaaatccag tgagtgtttc acatagtact gaaaaccagc aacacttggc atccgaccgc
    16261 ttgaagtatg agccttctca agcaaccctt gtttttctag ttccgccatg tcattacgaa
    16321 tggttgcact gctagagtta atagactctt gcaaggcttt tgatccgaca ggttcgtgcg
    16381 ttttggtaaa gatgtcaata atcagattta aaatatcctg ctgacgctct gtaaccatct
    16441 catcactcct ctctgtctga tcaattagca ctcaatgcat ccaagtgcta acttatgatt
    16501 ctattataca cccttagaag agaatgtcaa gacaaaaact caaaaaatta gcactctttt
    16561 atcaagagtg ctaaaaatca atctgaagac ctagaacctt gctattcatc tactcggtat
    16621 ttccttttta gtctgaaata accatgaatt agataagaaa tcagaaaagc atataaaaca
    16681 aaaataagaa agaaagacat agaaagatcc tctctaaaca aactcgtaca aacaacaaga
    16741 ccacctaaga aagtagttgt acaggaacga agtctagatc gcataacttc ccatttgagc
    16801 cccttactca tatagacttg atcctctgga agtaaatcag aagacgattt acgaagaatc
    16861 gaacaagaac ctgctcctat caattcccaa cctctatctc taaaatcttg cagttcacgc
    16921 ttatgctctt taagaaatct agaatcatag atacgataga taacatcgtc cggttgacct
    16981 tgctcaaaat agaacaaacc aaaacgacta gttctatacc tccaaccttt caaatgcatc
    17041 tcatgtaaat attcttcttt cttgtccaaa tcaacaatgg tgaaaatccg aaattctact
    17101 ctgctattca ttgtcttacc ccaaaattag aaaacatgcc tagcgttatt tattagataa
    17161 ttctttccac ttttgactca atctccaaaa aatataagaa atctgaatcg caaaaactat
    17221 caataaaacc caatctatta tgaaaatcaa aaacacttcc caactgaaag aactacctcc
    17281 agtgacaaac tttgagaaaa acggtagtag agctaaaaag agaagcaaaa taggaagcat
    17341 ccgcattgtt aaaatccttt tggcataaaa aaatctttat ttaaacgaaa atattatggc
    17401 aaaatttacg ccagtttttg aacggctgat gtagatattt tatactttca aaatgtttaa
    17461 atgtgattat ttatttttga aaaatagctc accagcccga ctgaaagtgc ttatagaatg
    17521 ataataagtc gcctgccgaa aacagcgaaa aatagcggtg ttatgcggag ataatctgac
    17581 gcgatgcgaa agtatattgc atacttattt tcaacaattt agcagagtat ttttataagt
    17641 gtgatataat agaagtataa tttgttctga tagtttattt tatggagaag tagattttta
    17701 gaatgcggag gattcaatat ggttgagttt ataaagtcta agaaagaaat gagtgaggag
    17761 gatattaaag caaatttcat cactcctgct attgtatcca aaggatggaa aaatggtgag
    17821 catatcgctt acgaagaata cttcactgat ggtcgaattg aagttagagg agataaggct
    17881 cgtcgtaaag aaggaaaaaa atcagactat tcactgtatt accaatttgg aactcgaatt
    17941 gcaattgttg aggcaaagga taataaacac agcgttcgag caggattaca acaagctatt
    18001 gaatatggag agattttaga tgttccattt gtttattctt cgaatggtga tggctttatt
    18061 gaacacgacc gtatcacgag agaagaacgt gagctggagt tagacgaatt ccctactcgt
    18121 gaagaattat tttctcgtat gacgaaggaa aaaggattga cgtacgaaat tacagaagct
    18181 atctcaactc catactatac agacgccttc tcaatgaaaa cgccacgcta ttatcagcaa
    18241 atagctatca accgtactat tgaaacagtt gccagaggac aaaaacgagt aatgtttgtg
    18301 atggcaacag gaacggggaa aacgttcatg gcttttcaaa ttattcatcg ccttcgaaaa
    18361 gctggtttgg ctaaacgagt tttattctta gcggatagaa acatcttagt agaccaaacg
    18421 atggctgaag actttaggcc attcgaaaag gtaatgacga aaattacacc aaaacttttg
    18481 actgctcctg aaaaattaaa ttcttttgaa atttatctag ggctttatca gcaactaact
    18541 ggtgaagatg gaactgaaac acattatcaa aaatttgaca aagacttctt tgatttaatc
    18601 gtaattgatg aagcgcaccg tggttcagct aaggaaaaca gtaactggcg taaggtaatt
    18661 gattatttca gttctgcgac acagattggg atgaccgcta ctcctaaaga aaccaagaat
    18721 gcttccaata cggaatactt tggtgagcca atctatactt atagtttaaa acagggaatc
    18781 gaggatggtt ttttggctcc atatcgtgtt atgagggtta atttagatgt ggatgtggat
    18841 ggttatcgtc cagaaactgg aaaagttgat gctaacggac aattaataga agataggtac
    18901 tacggcagga aagattttga taaaaccatt gtcattgatg atagaacgca aagagttgcc
    18961 aagtttgttt ctgattatat gaagcaaaac aatgcacgat ttgataaaac aattgttttt
    19021 ttgtgttgat attgaccatg ccgagcgaat gcgtgctgca cttgtaaaag agaatctaga
    19081 cttagtccaa gaagactatc gttatgtcat gcaagtaact ggtgacaacg ctgaaggaaa
    19141 agctcaactg gataacttta tggatgtcaa ttctaatttt cccgctattg taacaacgtc
    19201 taaattatta acgacaggag ttaatgctaa aacatgtcgt ttgattgttt tagactctaa
    19261 tatccaatcc atgactgaat ttaaacaaat tattggtcgt ggcacacgtc tttatcctca
    19321 aaaggggaaa gaatttttta cgattattga ttttcgaaat gttaccaatt tgtttgctga
    19381 ccctgatttt gatggtgatc cagtgaaggt gctggaaaca ggtgcgaaaa cagtcagtgg
    19441 ttctacgccc ggtttcgtag atgaggaagg tgacccagta gaaaaatata tcgttacaga
    19501 caagcaggtt accattctta attctactgt tcaagtattg gatgaaaacg ggaaactgat
    19561 taccgaaagc ctgaccgact acactcgaaa gaatatctta ggtagctacg ccactttgaa
    19621 cgattttatc acagtttggc atacggcaga taagaagaag cttatcttag acgaacttta
    19681 taaaaaagga gtttatctag atgctattcg agagtcggag ggaatatcag aacaagaaat
    19741 cgatgatttt gatttactcc taaaacttgc ctatggtcaa aaagaattaa ccaaaacgga
    19801 acgtatcaat aaactcaaac aaagcggata tttatataaa tatagtgagg aagcgcgtgc
    19861 tgttttggaa attttactga acaaatacat ggataaaggt attggagaac tcgaaagcat
    19921 tgaaacatta aaacttccag aatttcagat atatggtgga accttcaaaa tcatcaatac
    19981 ttattttgga gataaaaaac gatatttaca agcaattaaa gaattggagc aagagctatt
    20041 tacagtagct taatgaaagg aaagtatgtc aattacatca tttgtaaaaa gaattcaaga
    20101 tatcactcga aacgatgctg gtgttaatgg tgatgctcaa cgtattgagc aaatgtcttg
    20161 gttattattc ttaaaaattt atgatagccg tgaaatggtt tgggaattag aagaagacga
    20221 gtatgagtca attatcccag aggaattaaa atggcgaaat tgggctcatg ctcaaaatgg
    20281 ggaacgggta ttgacaggcg atgaattact tgattttgtc aataacaagt tattcaaaga
    20341 gttgaaagag cttgaaataa cttcaattat gcctattcga aaaacgattg ttaaatcagc
    20401 ttttgaagat gcgaacaact atatgaaaaa tggcgtcttg ttacgccaag tcatcaatgt
    20461 tattgatgaa gttgatttca atagccctga agatcgtcat tcgtttaatg atatttacga
    20521 aaaaattctt aaagatattc aaaatgctgg gaactcagga gaattttata cgccacgtgc
    20581 agcgactgat tttattgccg aagttcttaa cccaaaactt ggagaatcaa tggcagacct
    20641 tgcttgcgga acaggaggct tcttgacttc gactctgaac cgtttaagta gtcaacgtaa
    20701 aactagtgaa gataccaaaa aatataatac agctgttttt ggtattgaaa agaaagcatt
    20761 tcctcatctt ttagcagtta caaatctgtt tcttcacgaa attgatgacc ctaaaattgt
    20821 tcatggaaat actttagaga aaaatgttcg tgaatatacg gatgatgaaa aatttgacat
    20881 tattatgatg aatccacctt ttggagggtc agaattagaa acaataaaaa ataactttcc
    20941 agcagaatta cggagttctg aaacagctga tttatttatg gctgtcatta tgtatcgttt
    21001 gaaagaaaat ggtcgtgttg gagttatttt acctgatggt tttctatttg gtgaaggtgt
    21061 aaaaactcgc ttgaaacaaa aactggtaga tgagttcaac ttgcatacga ttattaggtt
    21121 gcctcatagt gtctttgcac cgtatacagg aatccatacg aacattcttt tctttgataa
    21181 aacaaagaaa acagaagaaa cttggtttta tcgtttagat atgccagatg gttataaaaa
    21241 tttctcgaaa actaagccga tgaagtcaga acacttcaat cctgttcgtg actggtggga
    21301 aaatcgtgaa gagattctgg aaggtaagtt ctacaaatct aaatcattta cacctagtga
    21361 attggctgag ttgaattata atttagacca gtgtggcttt ccaaaagagg aagaggaaat
    21421 cttaaatccc tttgagttga ttcagaatta tcaagcggaa agagcaactt taaatcataa
    21481 gattgataat gtattagctg atattttgca gttgttggag gacaaataat gacaccagaa
    21541 caacttaaag caagtattct ccaaagagcg atggaaggga aattagtgcc gcaaaatccc
    21601 aatgacgaac ctgcaagtga attattaaag agaattaaag ctgaaaaaga aaaacttatc
    21661 aatgaaggaa aaatcaaacg agataaaaag gaaactgaga tatttcgtgg tgatgatggg
    21721 aaacattatg ggaagtttgc tgatggaagt actcaagaaa ttgatgttcc ttatgatatt
    21781 cctgatactt gggagtgggt gaggttttct acattggttg aaattatcag aggtggctct
    21841 ccacgaccaa tcaaagatta tcttacttct gaagtagatg gaataaattg gataaaaata
    21901 ggtgatactg aaaagggtga aaagtatata aataatgtta aagaaaaaat caaaaaatca
    21961 gggcttaaca aaactagatt tgtaaaaaaa ggtacatttt tgttaactaa ttctatgagt
    22021 tttggtagac cttatatttt gaatgttgat ggtgcaatac acgatggatg gttggctatt
    22081 tcgaactatg aaaactcatt aaataaagat tacctattct atattctttc atcaaatgta
    22141 gtttattctc aatttctatc tctaattagt ggagctgttg tgaaaaactt gaatagtgat
    22201 aaagttgctt ctattcttat ccctctcccc ccactatccg aacaacaacg aatagtagaa
    22261 gcaatcgaat cagctttaga aaaagtagat gaatatgctg aaagttataa tagactagaa
    22321 cagctagata aagaatttcc agataaacta aaaaaatcta ttcttcaata tgctatgcaa
    22381 ggaaaattag ttgaacaaga cccaaatgat gaatcggtcg aagttttact tgaaaaaata
    22441 cgagcagaaa aacaaaaact ctttgaagaa ggcaagatta aaaagaaaga tttggacatt
    22501 tctattgttt cccaaggaga tgataactct tattatgagg aagtaccttg tgaaatacca
    22561 gaaagttggg aatgggtgag gttaaacgat ataacctcct acattcaaag agggaagtct
    22621 ccaaaatatt caaatattcc tatataccct gtgattgctc agaaatgtaa tcaatggtct
    22681 ggcttttcta ttgacttagc aagatttatt gatcccgaaa cagttcatag ttaccagaaa
    22741 gaaagattac taagggatgg tgatttaatg tggaattcaa caggtctagg tacgttagga
    22801 cgattagcta tttaccatga aaataaaaat ccttatgcct gggctgttgc agatagccat
    22861 gttacagtta taagagtctt atcgggggtt attaattgcc atttcatcta taattttctt
    22921 tcctctccta ttgttcagtc agttattgaa gaaaaagcgt cgggtagtac aaaacaaaaa
    22981 gaattattaa cgaaaactat aaaagaatat ttaatccccc tcccacccct tcctgaacaa
    23041 tccagaatcg ttgataaaat cgaacaattt ttcgcccata ttgacgcact aatttagtat
    23101 aatagtttta cgtgcggtgg aatttctatc aaacataaga taggagtttt acgttgaaag
    23161 attttgaatt acatttgaag aaagctggtt tagctgaaaa tactgtgcgt tcgtaccttt
    23221 atggtgttcg atttttcttg gagaattacg aactcaaaat ggaagattta tttgaataca
    23281 aaggatattt attagacaat tttaaaccta aaactgtcaa tttgcgatta caaggagtaa
    23341 ataaatatct gacttttatt ggtcatgatg atttgaaatt aaaatttgtc aaagtacagc
    23401 aaaaaccgtt tttagaagat gtcattagcc atgctgatta cctttttctt aaacgtagtt
    23461 tgaaaaaaga tggtatttta aaatggcatt ttgtcgtttg gtttttaggt gcaactgggg
    23521 ctcgtgtaag tgaacttatt aaactaaaag tagagcatgt agaaatcggt tattttgaca
    23581 tttactcaaa aggtggtaaa attcgtagac tgtacattcc taaaaaatta agaaatagtt
    23641 gtcttagttg gttagaatca gaaaatcgtc gaagtggtta tttatttttg aataaattta
    23701 acgaaccaat tacagcaaga ggagttgctc aacagttaaa aaattatgct gataaataca
    23761 aaatgaatcc taaagtaatt taccctcatt cttttaggca tttatttgct aagaattttt
    23821 tagcgaagta taatgatatt gccttgcttg cagatttgat gggacacgaa agtatagaaa
    23881 ctactcgaat ttatctaagg aaaacagcta ctgaacaaca aaatattgta gataaaattg
    23941 ttaattggta aaaaataaca ggtggtcaaa ctgactacct gctatttttg tgattatggc
    24001 tcttattatg ggaacaaaga cgaaacaacc tcttatccta tatatgaaat cccagaagca
    24061 tggagatata ttaaatttgc tagcctagtt aacttcagaa taggaaaaac tcctccacgc
    24121 agtgaagcta ctttttgggg aactgaaata ccttgggtat ctatatcaga tatgcctata
    24181 tctggttatg taactaatac gagagaatct atttctaaat tagcattaaa atcaaagaaa
    24241 atagacattt cacctaaagg aactttatta atgagtttta aattatctat tggaaaagtt
    24301 gccatattgg atattcctgc cactcataat gaagccatta tatcgatatt cccctatgct
    24361 aataaagaaa atattatcag agattactta atgatatttt tgccacttat ctctacttta
    24421 ggtgattcaa aagatgctat caaagggaaa acattaaata gtaccagtat ctccgaatta
    24481 ttaatcccta atttctaatc atgaagagat gaaaagaatc atttccaaag ttgatttact
    24541 ttttcaaaaa gtttctcaac tttttgagta ataagttcct gttcctcaaa aggagctaac
    24601 ggaattaata gctcgctcag tgtagtttta ggaatattat ataaagcttg acctgatagt
    24661 ttagtta
//
LOCUS       DAVHEW010000019        24139 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVHEW010000019 DAVHEW010000000
VERSION     DAVHEW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.019"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..112
                     /locus_tag="VVL53_000748"
     CDS             <1..112
                     /locus_tag="VVL53_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU4182948.1"
                     /translation="LEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
     gene            complement(229..576)
                     /locus_tag="VVL53_000749"
     CDS             complement(229..576)
                     /locus_tag="VVL53_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4182949.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYANDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(687..2462)
                     /gene="spxB"
                     /locus_tag="VVL53_000750"
     CDS             complement(687..2462)
                     /gene="spxB"
                     /locus_tag="VVL53_000750"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU4182950.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2669..4912)
                     /locus_tag="VVL53_000751"
     CDS             complement(2669..4912)
                     /locus_tag="VVL53_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU4182951.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4922..5293)
                     /locus_tag="VVL53_000752"
     CDS             complement(4922..5293)
                     /locus_tag="VVL53_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU4182952.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5304..5699)
                     /locus_tag="VVL53_000753"
     CDS             complement(5304..5699)
                     /locus_tag="VVL53_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU4182953.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVDKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5883..5981
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6065..6856
                     /gene="thiD"
                     /locus_tag="VVL53_000754"
     CDS             6065..6856
                     /gene="thiD"
                     /locus_tag="VVL53_000754"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU4182954.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7066..7698)
                     /locus_tag="VVL53_000755"
     CDS             complement(7066..7698)
                     /locus_tag="VVL53_000755"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU4182955.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(7691..8494)
                     /locus_tag="VVL53_000756"
     CDS             complement(7691..8494)
                     /locus_tag="VVL53_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU4182956.1"
                     /translation="MQEFTNPFPIGSSSLIHCITNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8494..9018)
                     /gene="thiW"
                     /locus_tag="VVL53_000757"
     CDS             complement(8494..9018)
                     /gene="thiW"
                     /locus_tag="VVL53_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU4182957.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9023..9715)
                     /gene="tenA"
                     /locus_tag="VVL53_000758"
     CDS             complement(9023..9715)
                     /gene="tenA"
                     /locus_tag="VVL53_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU4182958.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9726..10376)
                     /locus_tag="VVL53_000759"
     CDS             complement(9726..10376)
                     /locus_tag="VVL53_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU4182959.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALTLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSVSLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10378..11763)
                     /locus_tag="VVL53_000760"
     CDS             complement(10378..11763)
                     /locus_tag="VVL53_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182960.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11764..12324)
                     /locus_tag="VVL53_000761"
     CDS             complement(11764..12324)
                     /locus_tag="VVL53_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4182961.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12586..12674)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12883..13512)
                     /locus_tag="VVL53_000762"
     CDS             complement(12883..13512)
                     /locus_tag="VVL53_000762"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU4182962.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13514..14296)
                     /gene="thiM"
                     /locus_tag="VVL53_000763"
     CDS             complement(13514..14296)
                     /gene="thiM"
                     /locus_tag="VVL53_000763"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU4182963.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLKTAVLVY
                     NIAGEIAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14533..15201)
                     /locus_tag="VVL53_000764"
     CDS             complement(14533..15201)
                     /locus_tag="VVL53_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU4182964.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15416..15504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15758..16894)
                     /gene="lctO"
                     /locus_tag="VVL53_000765"
     CDS             complement(15758..16894)
                     /gene="lctO"
                     /locus_tag="VVL53_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU4182965.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17517..19007)
                     /gene="lysS"
                     /locus_tag="VVL53_000766"
     CDS             complement(17517..19007)
                     /gene="lysS"
                     /locus_tag="VVL53_000766"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU4182966.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19135..19281)
                     /locus_tag="VVL53_000767"
     CDS             complement(19135..19281)
                     /locus_tag="VVL53_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU4182967.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            19507..20175
                     /locus_tag="VVL53_000768"
     CDS             19507..20175
                     /locus_tag="VVL53_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4182968.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            20135..20812
                     /locus_tag="VVL53_000769"
     CDS             20135..20812
                     /locus_tag="VVL53_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4182969.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFRTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILILYFAVCYPISKLSTH
                     LEKHWRN"
     gene            20813..21571
                     /locus_tag="VVL53_000770"
     CDS             20813..21571
                     /locus_tag="VVL53_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4182970.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRYKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAITDRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            21583..22454
                     /locus_tag="VVL53_000771"
                     /pseudo
     CDS             21583..22454
                     /locus_tag="VVL53_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(22541..23188)
                     /locus_tag="VVL53_000772"
     CDS             complement(22541..23188)
                     /locus_tag="VVL53_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4182971.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(23185..23883)
                     /locus_tag="VVL53_000773"
     CDS             complement(23185..23883)
                     /locus_tag="VVL53_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182972.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(23893..>24139)
                     /locus_tag="VVL53_000774"
     CDS             complement(23893..>24139)
                     /locus_tag="VVL53_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4182973.1"
                     /translation="LTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPI
                     FQYMVNSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
BASE COUNT         7316 a         5256 c         4377 g         7190 t
ORIGIN      
        1 tttggaagat gtcatgaatc aactccaaga tgtcatacaa ggattggaga aggaggtgat
       61 aaagtccatc gttaatcgga gatggactag aatgcttttt gaaagcagat gagtattata
      121 tgcaatttct ttatataaaa agaccggatt gctccgatct ttcaatagtt catattctca
      181 atttctattt taaaaatagc taaggttaac gtcaaatgac tacgcgacct atttcatacg
      241 ataaaaatca agcactagac cagcaggtcc ttgaactaat aaggactctg ttccccaatc
      301 ggttacagtt ggtccgtgta aaacctttat accaagctcg ttcaaccgtt tgtagttctg
      361 gtctacatcc tcaacctcga tatgaataat gattcctgac tgaaagtttt ccaaaggaac
      421 caaatgattt tgtgacaaca taaggcagtg actaccaatc gtaaactgag caaaaccatc
      481 attagcataa tctgcctttt tatccaagat atgctccaag tcagcacaga cttggggaac
      541 atttgaaacg ataatatcta attgatttaa attcatttac tctcctccat aaaaagaccg
      601 gattgctccg atcttttaaa gttctgctct atgaaaatca aagaataaag tctacaagtt
      661 tcatatttga ttttcggcga gaggaattat ttaattgcgc gtgattgcaa tccttcttct
      721 tccaagaaga gacggaatgg tacgagttct tctgcttcgt atttttcctt gaaggctttg
      781 atagcttctt ctgagtgaag ttttggatcc aattcaagta cttctactgg aagtggacgg
      841 tgttgagtga tgcgagcatc gatgacaaca gttttacctt ctttgttcaa tttaacagct
      901 tctgcaacaa ctgcatcgat gtcttcgata cggtcaactg tgaatccaac agctccttga
      961 gcttccgcaa ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa gtgtttgttt
     1021 gtatcttcgt atttgttctt gatgaagccg tactcagcat ttgagaagac aaggttgata
     1081 actggaaggt cgtattgaac gtttgtgata acgtctgggt agcacatgtt gaatgctccg
     1141 tcacccatga tgttccatac ttggcgatct ggattgtctt tcttagcagc gataccacca
     1201 ggaagggcaa tacccattgt cgcaaagagt ggagatgtac gccacatgtt cttaggtgtc
     1261 atgtgaaggt gacgagtaga tgtttgagta gtgttaccta cgtcgattga gtagatagcg
     1321 tcttgatcag catgtttgtt gattgcattg taaacttgat acaattgcaa ttcaccctca
     1381 gttttacctt cgagtttgtt catgtaatca cgccagtttt ggttgttctt aacgtttgca
     1441 cgccaccatg gagttgattc aactgggttt actttgtcaa ggatagcttt agctgcttga
     1501 ccagcatcac caaggattga agcgtcaagg gcatgacgtt taccaagttt gtaagggtcg
     1561 atatcgactt ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc agcaaatggg
     1621 aagtttgaac caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt ggctggtttc
     1681 caaccaacac ggtaagcaga acctgtcaaa ccttcatagt tccattcgaa agcttcaaag
     1741 tttttaccag ttgtgatgat tggtgctttg attttacgtg acaattcagt aatcacttca
     1801 ccagctttaa caccaccaaa tccagcatag ataactgggc gttcagcatt gttcaagatt
     1861 tcaacagctt tgtcgatttc aacttcgttc aaagcaggag cgatgaatga gcgttcgtat
     1921 gaacctgaac cgtagtatga gttttcatcg atttcttgga aaccgaagtt tactggaatt
     1981 tcaacaacag ctggaccttt tttagaaact gcagcacggc aggcttcgtc aattactttt
     2041 ggcaattgct cagcgtaagc tacacgtttg ttgtaaacag cgataccgtt gtacattggg
     2101 ttttggttaa gctcttggaa agcatccatg ttcaattcgt taactggacg tgatccaagg
     2161 atcgctagga atggagtgtt atccatagct gcatcgtaaa caccgttaat caagtgagtc
     2221 gcacctggac cacctgaacc aactgcaacc ccgattgagc cgccgaattt agcttgcata
     2281 accgctgcaa gagcacctgt ctcttcgtgg cgaacttgta agaaacggat atctttgtct
     2341 tcagccaaag cgtccatcaa tgagctgagt gttcctgatg ggataccgta gattgtatct
     2401 acgccccatg ttttcaatac gttaagcatt gctgcagatg cagtaatttt cccttgagtc
     2461 ataatgataa ctctccttca atttttttaa acttggagaa tacgattaca tagaattgga
     2521 aacgttctcc aaatttttac tattccactg tatcatattt atgctgactt ttctaaaaat
     2581 ctgctcaaaa ctctctattc tctattctaa tacagttttg aaagttctgt catttctgtt
     2641 ttataacaaa gaaatctagt cattactttt agtctatttt actaaaattt aacagaaggg
     2701 aactggtcag aacagataca gaactaaagg ccatggctag acctgccaat tctgggttga
     2761 gagccagtcc aacacctgaa aagactcctg ctgcaatcgg aattccgaca acattgtaga
     2821 taaaagccca gaaaagattg agtagaattc gatgaaaggt tttcttactc atatcaaagg
     2881 cacgaaccac tcctaaaaga ttattggttg tcaacaccaa atctgctgac tcgatggcga
     2941 tatctgttcc agctcccata gcaatcccca catctgctac actaagggca ggagcgtcat
     3001 tgataccgtc cccaacaaag gctactttcc ctgactgttg cagtttatgg atttcatggg
     3061 ctttttcttc tggcaagacg cctgcaatga cctcttcaat tccgatttga tctgcaatag
     3121 cacgcgccac accagcattg tctcctgtca gcatgactgt tcggagacca cgttttttta
     3181 gctgactgat ggctagctta gcattttcct taggaatatc ttgcaaagca agcaagcctt
     3241 tgatttcatt gtcaacagct aagaacacaa ctgtcttagc ttctttttct agttcttcta
     3301 gtttatcttg ataagtatta gaaatatcca tgccatccag cattttagca tttccaagta
     3361 aaacttgttt tccattgatt cgccctgaaa cacctttccc gtgcaaggac tgaaaatttt
     3421 caacagtttg aaactcaagt ccagcttcac tcgctcgctt aacgatagcc tcagccagtg
     3481 ggtgttgaga agcatcttcc aaggaggctg ccaacccaaa cacttctact tcgtcgccga
     3541 tgacatctgt taccacaggt ttcccttccg tcaaagtccc ggtcttatca aagacaaggg
     3601 tttgaacttt ctggatttcc tgtaagacag ttccattttt gaggagaacc cccatcttgg
     3661 cactacgtcc tgtccccacc ataagggctg tcggtgttgc aagtcccaag gcacaaggac
     3721 aggcgataat caaaaccgcc actccgtaga gaagagagga cacaaagcta gctccaagca
     3781 caaccacact atccctgagc aagacgaacc aaacccaaaa ggtcatgatt cctaaaatga
     3841 caactactgg gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc ggcgcacgac
     3901 ttgtctgagc tttcttcaca aaatccacaa tctgagccaa aacagtctct gagccaactt
     3961 tttctgctct aaagacaagc gttccactat gattgatggt tgagccaatg acagtatctc
     4021 caactgtctt gtccacaggc agactctcac ctgtcaccat ggattcgtca atactagaga
     4081 caccttctac tacgacacca tcaacagcaa tcttttcacc gggacgcact cgaatcaggt
     4141 cgcctacctt gacttgttcc aaaggaactt ggacataact atcatcactc aagacttctg
     4201 cggttttagc ttgcaagtcc agtaatttct ccacagcttg ggacgtattt tttctcattt
     4261 tttcctcaaa aactgctccc aaaagaacga aaaagaggat aaatccagca ctttcgaagt
     4321 aaacagggag accagcaaag agagcaacta ggctatagaa ataagccact agagttccca
     4381 gcgcaaccaa ggtatccatg ttggcattgt gctttttaaa actggcccaa gcactctgga
     4441 tatatggctt acctgcaact aacataatag gcgttgttgc tagaaaggtt ccccaatgca
     4501 tgacttgatg actaatgcta cctgtcaaca tcccaatcat gagaatcaca agaggcacag
     4561 taaagatact agtaatccaa aaacgttgca ggagagatag agattttcga gtcttctcaa
     4621 cgactgtata gcttcccttt tgcatcttca tgccacaaga aaattcatgt cgccctaatt
     4681 cttgaggcgt aaaacgaatg actttctcct catctacgcc gattggttcc aagatacctt
     4741 cttcttcaaa cagaatttcc ttataacagt ttgaaggagt agcacgatga aaggtaatct
     4801 cagctggaat tcccttttga agctggatat gggctggatg atagcctttt tcagctcgga
     4861 tacggatttt ttgaatgcca ttttctaagc ttgctttcac aatttctgtc atagtctcca
     4921 cctactctac aatcatcttg ccgtgcatca tgttcatacc acaagcaaag ccaaactctc
     4981 cagcctgttc aggcgtgatt tccactacat actcttcccc cattggcagg ttcgcatgta
     5041 caccaaaatc tggaaaaaca atttgatcca gacatggtga aggatccttg cggtcaaaga
     5101 caatgcgtgc tggcactgat ttcttgagga caatcaactc aggagtatag cctcccatga
     5161 cttccactcg aatctcttgg tatccgtttt tttgctgggc tttttgtcca gatttttcag
     5221 gctttttgaa aaaccaaaac aagataaacg cgataagggc aatacaaata atggttacaa
     5281 tactatttaa catgacgtct cctttacata caattacatc ttacttctgt tacagcactt
     5341 gatttcttct ctgaaatcac agcttccaag tcttccaagt cagtctgagt aaattcacat
     5401 tctacaatca agtcagccaa caaattccta atcctacggg aacaaacctt gtctttgata
     5461 tcttggacaa gtaaatcccg actttggtct agagttaaaa gggctgaata aacaaaggac
     5521 ttgccttctt ttttccgagt caaacactct ttatcaacca gacgagccaa aagtgtctga
     5581 accgtggact tggaccagtc aaaccgctct gccaaaaccc taatcaaatc tgtactggtc
     5641 tgctccccct gcatccaaat aatcttcatg acctgccatt ctgcatctga aatctgcatt
     5701 accatacctc caaaatctac atttgtcaat tacactcatc agtatactct taaaatctac
     5761 atttgtcaat tatagaaata atattttctt cgaaaaatag aattttaatc atttgaaaaa
     5821 cgatttgcag tcaaatatta ctatataaac aataaaaata tgctatacta aagaaaaaag
     5881 aaaacaacca ctaggggtgc gtaaagctga gattaacgac tgttagatcc ctctgactca
     5941 atctaggtaa tgctagctga tggaagtgga aatgataatg gggactagca gtcttctatt
     6001 gcctttctaa aacagactag cttgttctta agaatacaaa cttcagttgg ttgggaggtt
     6061 ttagatgact tatttacccg ttgctttgac cattgcaggg actgacccta gtggtggtgc
     6121 tggcattatg gcagatttaa agtcattcca agcgagagat gtctatggaa tggctgttgt
     6181 aaccagtctt gtcgctcaaa ataccagagg tgttcagcta atcgagcacg tttctcctca
     6241 aatgttgaaa gcccaattgg agagtgtctt ttctgatatt ccacctcagg ctgtaaaaac
     6301 tggaatgttg gctactactg aaatcatgga aatcatccaa ccctatctta aaaaactgga
     6361 ttgtccctat gtccttgatc ctgttatggt tgctacaagt ggagatgcct tgattgactc
     6421 aaatgctaga gactatctca aaacaaactt actacctcta gcaactatta ttacgccaaa
     6481 tcttcctgaa gcagaagaga ttgttggttt ttcaatccat gaccccgaag acatgcagcg
     6541 tgctggtcgc ctgattttaa aagaatttgg tcctcagtct gtggttatca aaggcggaca
     6601 tctcaaaggt ggtgctaaag atttcctctt taccaagaat gaacaatttg tctgggaaag
     6661 cccacgaatt caaacctgtc acacccatgg tactggatgt acctttgctg cagtgattac
     6721 tgctgaacta gccaagggca agagtcttta ccaggcagtt gataaggcca aggcctttat
     6781 cacaaaagct attcaagatg cccctcaact cggtcatggt tctggtccag tcaaccatac
     6841 aacttttaaa gattaagaaa aaaaactctc tagttcccac tttaagggaa ttagagagtt
     6901 tttatactct tcgaaaatct cttcaaacta cgtcagcttc catctgcagc ctcaaaacac
     6961 tgttttgagc tgacttcgtc agtcttatct aaaacctcaa ggcagtactt tgagcaacct
     7021 gcgactagct ttctagttta ctctttgatt ttcattgagt attaattagg aaagaatgtt
     7081 atgcaacttt tttaaaaagg cttgcgtttt tgcctcaata tcttctgctt gcatcaaatc
     7141 acgtacaaca gctacaccag ctatgccagt gcccataagc tgatcaatat tctccgaagt
     7201 caagcctcca atagcaacta ctggaatggc aaccgtttgg caaattgttt tcaaggtcga
     7261 tatcagagta atgggcgcat tttccttggt ggtggttggg aaaatggctc ctgtacccaa
     7321 gtaatctgca cctgatttct ccgcttccag agctctttta accgttttag cggtgacacc
     7381 gaggattttt tcaggaccca agactttgcg agctaccgaa actggtaatt catcatctcc
     7441 gatatgcaga cctgctgcat caaccgcaag acaaacatcc aaccgatcat cgattatcaa
     7501 gggtacctga taagcatctg ttatttcctt gacttgtttt gccagttgat aatattgatt
     7561 ggttgtgaga tttttttctc gcaattggac tatggtaacc cctgaacggc aggccgtctc
     7621 aacttttgca agaaagcttt ccacggaatc ttgatagcga ttggttacca gatatagtct
     7681 aagtgcttct ctattcataa acctctcctt tgatggtatc tagccaattt tcatctcttc
     7741 ttaggagcga aagctgattg agtacttggt aacgaaattc ttccaatccc attccttgaa
     7801 caactatttt ctcagcagcg atattgagat aagagactgc taagcaagaa gcttcaaaac
     7861 cagtctttcc ttggctgaga aaaacagctg ttaaggctcc aaccaagtct cctgtccctg
     7921 ttatccagtc taattcagta cagccatttc ccagtacagc gacctgattt ttcgaaacga
     7981 cgaggtcctt gggacctgtg actaagaaag acataccagg ataggtctga caccagtctt
     8041 tcaagacttg aagcaaatcc tccgtttctt gatctttagc actcgcatcg accccaacgc
     8101 cgtggtgctt taatccaaca agacttcgaa tttctgacat gtttccttta aggaccgtag
     8161 gtctatagtc taaaaggtct ttaactaagc tcttacgaat ggatgaagtc gttacgccaa
     8221 ccgcatctac taccatcggg agagaagatt ggtttgcata cgaagctgcc atgcggattg
     8281 ctttttcctt ctcagctgac aaatgcccca aattgatgaa gagagcctga ctttgcttag
     8341 taaaatcaag aacttcacgg gaatcatctg ccatgacagg tttgcatccc agagccaaaa
     8401 tcccatttgc cagcatctca caagaaatct cattggtaat gcagtgaatg agggaactag
     8461 agcctatagg aaagggattt gtaaattcct gcatcagtct atcctttcac taaagaaata
     8521 tccctgcact tttttaaaga attcctgctt gattaaaaat cgaaaggcaa taaaggaaat
     8581 cgctgtacca atcaaggttg ctccgaaaaa tcgaggcgtg tagataaacc agctaagctt
     8641 agcagctgat cctgtaaaga gtaccataac aggataggaa acaatggaac caataatacc
     8701 tgttcccaaa atctctccta gagcagaata gtgaaatttt cgaccgtact tataaaagag
     8761 acctgctaga agggctccaa aagtcgctcc tgtgagagct aaaggcggaa tcccttgagt
     8821 cgtcatacgg ataaaggctg tgactgtagc catagccaag gcataaacag gtcccatcat
     8881 gattcctgct agaatattga ctacactgga catcggtgcc attccctcaa ttcgaaagat
     8941 aggtgtaagg actacatcaa gggcaatcat catagataaa atggttaatt tgtgaacttg
     9001 taattggtgc tttctcatgc ttctattctt ctcctttttc taaagactgt aaatcgctct
     9061 tccatgtctg gtgttggtag gccatttccc aaaacttggc ttccatatga acactgatgt
     9121 ggaaggcatc tagcattttt tgcttgtctg tctcgtcact ttctcgatag agctgattga
     9181 ccagtgctcc ctcctctctg atctgttgct ctaactcatc cgtaatataa gtttcaatcc
     9241 attgttgata gagaggattt ggtgatggtt taagattaag tgatttgcct atatcatggt
     9301 ataaccaagg acaaggaagc aagcttgcaa aagcgatggc taagttcggt tctgcaaatt
     9361 gcctataaat atgagaaatg taatgataac aggttggagc gattggatgt tgctccattt
     9421 cctggtcgct gatttccaat tccttgaaaa attgttggcg aataaataac tcaccctcca
     9481 ctaaaccctg agcattttgt ttcaagagtc ttttcatctc ttggtttgaa gtcttatcag
     9541 ccaaaagatg atagatttct gagaaagcct tcagatagta ggcatcctga atcaggtaat
     9601 agcggaaaat ggcaggttct aaattcccct cttgtaattg taaaataaag ggatgatgaa
     9661 aggaagcctg ccaagctttc ttggataatt ccatcgcaat atctgtaaat tccataataa
     9721 ctcctttata aaaatagact ggtttgaagc aataaaaaga aaagcaggta gattaatttt
     9781 gtttttttag gaatataaaa agtccgatag ctattcttca actgtgcatg ttcgtcatat
     9841 ccgtgagcag atagagctct caggtaaaga tggcgccacc taaagactgt catcagaacc
     9901 ttactgtaaa tcaagggcga ccaaaaatgt agttcttgac cacgtaatag gcaagcttct
     9961 ttgagggact tgatttcttg ctgaatgaga ggaaaagaat tgaataccac aatcaaggca
    10021 taggaccaag agcgtgatag ccccttttga gccaagtaca agagaagctc ttttagtgaa
    10081 acagaggaaa caaagacaag gccgatacaa actgtcacaa aggccctcgt tccaagcatg
    10141 actgcctgtg aagcatctcc gtgtaactga actgcccagt agttggcaaa agatggtaaa
    10201 atggcaagta tgatcatcca agctaacatt ttaaatcgac ggtaatagag cataaagaga
    10261 atacaaaatg cgactaccga aagagtcaga gcaatcgaag gaatgaaaga tgtttccaag
    10321 gataaaatca gcaagaagag actgataatc ggtgtctggg ttgctacttt gaccatacta
    10381 tctcacctcc ccttgggtat tgctactctg agatgtaagt ggtttggtaa tggtcacttc
    10441 tttcacatgc cgaagaccct gactagtcat ctcaatccaa taatcaacca cagaaatcaa
    10501 agggtctaaa cgatgactaa tgagcagaaa acttcttcct tgattcctct cctccacaat
    10561 ccacttgcaa aaataatggc aggctctatc atccaaacct gcaaaaggtt catctagcaa
    10621 gatcacggaa gccttactgg tcaagatggt caggagctga agaatttttt gctgaccacc
    10681 acttaattga tagggactct tatcgactgc ctgctccaaa tcaaaatatc gtaaagcttg
    10741 aaaaatccgc tgatttcttt cagaatcagg tccatctaat tgaagctcct ctcgcagact
    10801 gactcggata aactgcttct cagcttcctg aacaacacca gtcagatcac gatacaaact
    10861 ctttttcttt ttcaggaccg aacccttcca agtaatgctc cccttatact tttgaaattg
    10921 aagaatagac cgaaagaggg ttgatttccc gacaccattg tcacccagga tacaggaaat
    10981 cccttgatag aatgtgaaat cagcaattga aaagaggggg cgattaccaa gctcaccagt
    11041 cacacggttc atatggaata gttccgggct agaagcaact tcctttgaag caacctgtgt
    11101 catctcatag gaagggattt gaaacacttc ccttagtttt ccgtctctta gctccaccat
    11161 atggtcgata taggctttat agtcagataa atcatggtcg cacaaaataa ctgtcttccc
    11221 atcatagacc aactctttta gaatctccaa tatctcgatt ctgctcttgc ggtcaatgga
    11281 agcgaagggc tcatccaaga gatagaccct aggattcatg gcaaagagga cagccagcgc
    11341 tgctttttgc ttttccccac ctgataagtg atggatgaga cggtgcaaga tgtccttgca
    11401 acgacattgc tggacaacct ctgctatttt agaatcaatt tcctgaaggt gatagccgat
    11461 attttccatg gtaaaaacca actcctcaaa caagctctcc atggtaaatt gatgattagg
    11521 attttgcaag agaataccaa ccgtctggac acgttcgacg atagaaagct gactgacctc
    11581 gctcccatct atcaggactt gaccgctata gggaagagaa ctaacttggg caatcatttg
    11641 aaagaggctg gattttccag acccactact cccaactaac aaggtaaagg cttgcgcatg
    11701 aaaagtaaaa tcaaacggct cagagaagat tggggactga atcgctcgta gttccagacc
    11761 catctatgct tttcctccag ttgcaaactg atgatagagt ttgacaatgg cacgaaccaa
    11821 gatggtacag aagaaataaa cagaaataaa acgtaccaca agcaaggaaa ggacaaacgg
    11881 aagggaaaag gcgtagtaac ctaacttaat gtattcatag acaaagctaa caagcgtaat
    11941 cccaatacta ttagcagtta gagagagcca actttcatag cgattcttag ttacgataaa
    12001 accaaattca cttcccaaac cttgaacaaa gccagacaaa agagctccta gaccaaattg
    12061 gctaccataa aggacttcag caagcgcagc tagcacttct ccaatcgttg cacttccgac
    12121 tctcggaaca aagatggcag caatgggcgc agccatacac cagagaccga agaggatttc
    12181 attggcaaag gcctgcaaac caagaggtgt taagagtaga ctgagaatat tatacacata
    12241 tcctgaacca acgaaaaccc caccaaaaaa gatagacaag aaagcaagca agataacatc
    12301 ttttaactgc cattttttca acataaaaaa ctcctttttt taaagaaaag tgaggcactc
    12361 aagaagaccg acctaaatac tttgtatagc agactgaatt tagaacagta cacaagaaca
    12421 ctaaaatatt tctagaaatt aatttgaatt ttctaattga tttgttcgca tcttatttca
    12481 atctactata tcatcttcat ccagtttcgt aaaagaaaaa actctaatta cagatacaaa
    12541 ttagagttca gcttacaaga ttagacagtt cttttcgaca tacgaaaaaa acatttcaca
    12601 tttcccttcg ccagtcttaa ctgtatcagg ttcaatgggt atcatctcag cctaaagcac
    12661 cccaaatgtc tttattattt aattatgtga ttattataac acacatttta tactagttca
    12721 agaaattgaa ctggaaatac agccttgcac tcacaaagac agcagatctt tcttttgcaa
    12781 aaaacaaatg acctgtttga tgaattagcc attcaagctg aatctggaca tagcttttta
    12841 aaaaaggaaa atcctactta cttagaatcc aaggatagat atctattgtt cactcatttc
    12901 ccgaacagtt ttttctatat tttttgcata cgatattgcc gaaatgattg aaacgccatc
    12961 catattggtc tttataatgt ctttaatatg tttcgtctgt atcccaccaa ttgcaactaa
    13021 aggcatttgt ggcaatagtt ttctcatcaa ttcaagacct tcataaccta tagtaccacc
    13081 agcatcatcc tttgactggg taccaaatac aggcccaaca cctacataat ctacatattc
    13141 aacttttgat tgttgaaatt cttcttcgtt tcttatagaa agaccaatta ttttatctgg
    13201 catcaatttt ctaatttcat caacaccaat atcatcttga cctacatgta cgccatcggc
    13261 gtcaatttcc attgctaaat ctatatcgtc attaacgata aatggaacat tgtatttttt
    13321 acaaagttct ttaatttgga tagctagctc aagtttttct aagccttcta aagcaccctc
    13381 acctttttct cgaaattgaa ataaggttat accacctttt aaggcttcct caacgactgt
    13441 atatagattt tttccttggc aagtagtcgt tccacaaata aaatatagtt ttagtaattc
    13501 tttatgaaac atcttacttc actcttttga attcctttac atcttcatct gtaatctcgt
    13561 ataaggcatt tataaattca actttaaatg tcccaggaag atgtccattt ggacgttttt
    13621 ctgctatttc tccagcgata ttgtaaacca acactgctgt ttttaatgat ttcaattctt
    13681 gacctttttc tagtccgata aagcttgcta ctacagctcc taataagcat cctgtcccaa
    13741 tgactttcgg catcatagca ctaccattat gaatcattac cacttctcca ttaacagcaa
    13801 tggcatccac ttcacctgtt actactattg gaatattgaa cttctcattt gctgctagag
    13861 caatttcgtc aatattatct acgcccgcac tatctactcc tttagatgcc acatctattc
    13921 ctactaaaga ggcaatctcg ccagcatttc ctctaatcgc tgctagttta taattgttga
    13981 ttagatcatc tgctactttt tttctatatt ctcctgctcc acaggctaca ggatctaaaa
    14041 ctgctgggac attatatttc tctgcaattt tcagagcagc ttggtataat ttccaatttt
    14101 catctgtcaa tgttcctatg tttattaata aaccaccagc atactttaac aaatcctcta
    14161 aatctgctgg aaactcactc atggctggtg aggcgcccag tgctactaat ccatttgctg
    14221 tgaaattttt tactacatca ttggttatac aaatgaccaa tggtgctttt tcttttaata
    14281 attttaaact tgtcatattg aaatccttcc ttttcacttt atacgatcta ctaatttcga
    14341 tttatcttta gttgagaatt tttttcattt acattgaatg atttatactc aatgaaaatc
    14401 aaagagcaaa ctaggaggct aaccgcaggt tgctcaaaac actgttttga ggttgtggat
    14461 agaactgacg tggtttgaag agattttcga agagtcttac ctcatcaaat ttgtaaatat
    14521 catgagcctt ctctagacat cgtaaccaat atcaaaaaaa gctaattcta aagcgactgc
    14581 ttgattccag cgttgctgaa gttctgtcaa atcttctcga tttttaccga cacgattgag
    14641 ttcgtcaacc agaaattgaa cccactctgc aaagaaagga cctctgtgga gattgatcca
    14701 ttccgaatga atatagactt caggtaaagc caaatcttta gaaccccagt ctaaatagag
    14761 accttctgca atgaccagca tgaccaaaag atgggcatag tctgatgaag ccaccgccga
    14821 atacattaga tcctgaaagg cttttgttac agggtgcaaa gtcacttcta gatagtcatt
    14881 ctctgctact tttaactctt taaaagcctt ttggaaataa ccatcttcat ctgcttcaag
    14941 aaagcctagt tgcttggcaa aacgaagctt ggattcaagt ttatctgcgt gagctacgca
    15001 ggcacccagc atggataaga aggcatcaaa gaagtgataa tcttgaatca gatagtcctt
    15061 taagacctta ttctcaattg tccccgcaaa aagttcctta acaaaacgat gattgattgc
    15121 agcctgccaa tccttctgac tgctttttaa taattctcca acagtcaaac ctggctgaaa
    15181 tgcatagtct tgtgtttcca tatttacttc tcctctcttt acttgttagt aattaataaa
    15241 acaccaagaa atatcaagca aaatcgtaat tccacttgat ccttttaaag cacatcgaga
    15301 gcatttgcag agagctaact aaacaagcct atccagttta tataaacaaa aaactccaat
    15361 tacaatcaag aattagagtt gacttacaag attagaccgt tcatttcacc atacgaaaaa
    15421 actgttcaca tttccctttg ccagtcttaa ctgtatcagg ttcaatgggt attatctcag
    15481 cctaaagcac cccaaatgtc tctattattt aactactgaa ccagtatagc aaaaaatgaa
    15541 agccctagca agatatttga ccgaaaaata tctttatata taatatattg aaactagaat
    15601 agtacacctc tacttataaa acattgttag aaatcgattt gactgtcctg attgatttgt
    15661 cctattctta tttcatttta ctatagtttt cgatagcaat ttattcttcc aatacacgaa
    15721 gaaaaacctc cacattcagt ggaggcaatc tgttttatca atacaatttt aagtcacgag
    15781 ggtcaactgg gaaggttggg ttgtatggat tgtgacggag cttgaagtgt ttgacatctt
    15841 caatggtctg agttccagac aattgcataa ctgtcttcaa ttccgcattc aagtgttcaa
    15901 agacttgacg cacaccgaca ctaccaccga gagccaagcc atagatgaca gggcgtccaa
    15961 tagcaaccaa gtctgctcct gatgccaagg ctttaaagac gtgttgacca cgacgaacac
    16021 cagagtcaaa gacaatcggc acacgtctat caactgcttc tgccacttct tgaagcgagt
    16081 caaaggcagc tggtccaccg tcgatttgac gaccaccgtg gttggttacc cagataccag
    16141 aagctcctgc agcaagcgaa cgttcaacgt cctcacggca ttgtggtccc ttgacataca
    16201 caggaagacc agagtattca gcgataaatt ctacatcgcg tggagacaag cgttgtttag
    16261 ctgatttgta aacaaagtcc attgatttac cagcaccttc tggcaggtat tcttcaacaa
    16321 tcggcatgcc aactgggaag acaaaaccat tacgcttatc cacttcacga ttccccccta
    16381 cagtagcatc tgccgtcaag acaatcgctt tataaccttc agccttcaca cggtccatga
    16441 tgtggcggtt gataccgtca tccttactaa agtaaaattg aaaccaatga ggtgtccctt
    16501 ggagggcttc agaaatctct ggaaggtcaa cagtagagta agaactggtt gtataaagag
    16561 aaccaaactc atgcacacca cgcgcagtcg ccacttcccc ctgttcattt gccaatttat
    16621 gagccgcaac aggtgccata atgattggag aagatagttt ttcacctgca aattcaatct
    16681 ctgtacttgg attttctaca ttgcaaagtg tatgaggaac gatgagcttg tggttaaagg
    16741 cacggatatt ctctcttaaa gtgaaagtat cttccgcccc actagcgata tagccaaatg
    16801 ctgctttagg aataacttgt tgcgccattg gctccaaatc ataggtattg atgaagtcta
    16861 catgaccttc tgcattgctt gttttgtatg acataaactg tcctcctcga ttaagtaagc
    16921 gtttgctttt tactatacaa ctataataac tcttttttca atcattttca agagttttgc
    16981 ttaaaaacca tcttattttt tcatacaata tactagccct actttcctta aactctaaac
    17041 cttagtctat attaagtgaa tcgttgagat tttttgctgc caggcatttg aactactttt
    17101 tatttgctga ttctatctac taatactata aaaaacaagt gaataagaca ccagaacatc
    17161 ctctaatagg acctatggac ttatatcaat cccatatata ctgtttaagc aagatactat
    17221 cctagttgac gctaacttcc tatcccactt attaattttt tatttatagt gaatctgaac
    17281 aaatcaattg gggaatccaa gtcaatttct agtacttttt gaaaagtttc agcgtactat
    17341 cgtaaaatga aataaaacat gcacaaattg cttagggaat ttaaaccaat ttctaacaat
    17401 gtcttagaaa tcaaagtgta ctatttcaat ttcaatatac tacacttcat tttctcctta
    17461 aattcagtct ctttttgaat caaaaaatcc tctgataaga cccagaggat aagaatttat
    17521 ttcattgttg ggaagagcaa tacatcacgg atagttgttg tatcagtgag gagcatgcag
    17581 agacggtcga taccgattcc caaaccacct gttggtggca taccgtattc aagagcttca
    17641 atgtagtcat agtcgattcc tgtcgcttca tcatcaccaa gttctttggc tttagcttgg
    17701 gcttcaaaac ggctaagttg gtcgattggg tcgttcaact cagtaaaggc attaccgtac
    17761 tccttagtca tgataaagag ctcgaaacgg tcagtaaagc gttggtcttc aggatttttc
    17821 ttagcgagtg gagatacagc tactggatgt ccatagacaa aggttggttg gattaaagtt
    17881 tcttcaacaa actcttcaaa gaaggcattg atgatgtgac caacctcagt gtagtgtttc
    17941 tcaactggaa ctttcttctc agcagcgata gctttagctt cttccaaagt catgtcttgc
    18001 cagaaatcga caccagtaat ttctctgata gcatccacca tatgaacacg cttaaatggt
    18061 tcgttaatct tgatttcagt accttggtag ttgactgggc catcaccttt gactgattta
    18121 gcagcgtgtt ggataatgcc ttcagtcaag tccatgatgt cttggaagtc tgcataagct
    18181 tggtaaactt cgatagaagt gaactcaggg ttatgagtag cgtccattcc ttcgttacgg
    18241 aagatacggc caatttcata gacacgttcc ataccaccca cgataaggcg ttttaagtga
    18301 agctcagtcg cgatacgaag caccatgtca atgttttggg cattgtggtg ggtgataaat
    18361 ggacgggcag cagcaccacc ggcttcatta tgaagaacag gtgtttccac ttcaaggaat
    18421 cctttttggt caaggtaacg acggatttca gagatgattt ttgaacgagt gacaaagcgt
    18481 tcaaagcttt cacgattaga aatcaagtca aggtaacgtt tacggtaaat tgtttcaacg
    18541 tctgtcaaac catggaattt ctcaggaaga ggacgaagag ccttagacaa gtgtgtgatg
    18601 tgggttgcct tgatagagag ttctcccata tccgtacgca tcacttcacc ttcgacacca
    18661 aggaagtcac caaggtctgc ttttttgaag atttcgtagt tttcttcacc gacagcatcc
    18721 ttacgaacgt agatctgaat ctggccttcg cggtcttgaa ggtgggcaaa accaactttt
    18781 cctttaccac gtttggttat caagcgtcct gcgatagtag ctgtttcgtt tttatcgtgt
    18841 aattgttctt tatcgaggtt ggcatattta tcttttaatt cttgtgaatt tgcagtacgt
    18901 tcaaaacgtt ttccgaaagg atcgattcct tgttcgcgga gcgcagccat tttttcacgg
    18961 cgaacgatct gctggtcatt tagttcttcc atatgttctg tagacatggg atcctcctag
    19021 ttttactcaa aattgaatac gatgagtcat tttttgcgac actcccattt tatcataaat
    19081 aagcaagaaa ttcacggata atctttaaaa actaaaaaga acctgacgct aaaatcactg
    19141 gaaagaagtg aaagtatctc ccgccccact agcgatatag ccaaatgctg ctttaggaat
    19201 aacttgttgc gccattggct ccaaatcata ggtattgatg aaatctacat gaccttctgc
    19261 attgcttgtt ttgtatgaca taaaatgtcc tccttaataa gtaagcgttt actttgtgta
    19321 ttacaaaaat atcttaactc tttttcaaaa cttttaaaat attttgtttg gaaatttcag
    19381 aaattttatg tctatgataa aaatccttat aacggcaata aaaaatagat attatccaaa
    19441 gaagatttta agtgctacaa taactgtatt atttctagat gggaggttct atttttggat
    19501 tgatccattg ttgaacaata tctaccacta tatcaaaagg cattctttct gaccttgcat
    19561 attgcagttt ggggaatttt gggatccttt ctgctcggtt taatcgttag tatcatccga
    19621 cattatcgaa tccttgtttt ggcgcaagta gcgacagcct acattgaatt gtcacgtaat
    19681 acgccccttt tgattcaact cttctttctc tacttcggtc ttccccgaat cgggattgtc
    19741 ctatcttcag aagtctgtgc aacgcttggg cttgtctttt taggaggctc ctatatggca
    19801 gaatctttcc gaagtgggct ggaagccatc agtcaaaccc agcaggagat tggcctcgct
    19861 attggtctga cacctctaca ggtcttttac tatgtggttc ttccgcaagc aacagcggtg
    19921 gcactcccct cctttagtgc caatgtcatt ttccttatca aggaaacctc tgttttctca
    19981 gcagtggctt tggccgacct catgtacgtc gccaaggatt tgattggtct ctactatgag
    20041 acagacattg cgctagctat gttggtagtt gcttatctaa tcatgctgct acccatctca
    20101 ctggtcttta gctggataga aaggaggctc cgccatgcag gattcgggaa tccaagtact
    20161 ctttcaagga aataatctcc tgagaatctt acagggattg ggcgttacga ttgggatatc
    20221 catcctgtct gtcctcttat ccatgatgtt cagaacagtc atgggaatca tcatgacctc
    20281 ccattctaga atcatacgat ttttaacacg attgtatctg gaatttatcc gtatcatgcc
    20341 ccagctggtg ctactcttca tcgtttactt tggcttggct cgaaacttta atatcaatat
    20401 ctcaggtgag acttcagcta ttatcgtttt taccctctgg ggaacagctg aaatgggaga
    20461 cttggtacgt ggagctatca cttctctccc taaacatcag tttgaaagtg gacaggcact
    20521 cggcttgact aatgttcaac tttactacca catcatcatc ccacaagtct taagaagact
    20581 gctaccgcag gctatcaatc ttgtcactcg gatgattaaa accacttcat tagttgtttt
    20641 gattggggtt gtggaagtga ccaaagttgg acaacaaatc atcgatagca atcgcctgac
    20701 catcccaact gcttcatttt ggatttatgg aaccattcta atcttatatt tcgcagtttg
    20761 ctaccctatt tccaaactat ccactcactt agaaaaacat tggagaaact aaatgtctga
    20821 aactatctta gaaatcaagg aactaaaaaa atccttcgga gacaatccca tcctccaagg
    20881 actttctcta gaaatcaaaa aaggggaagt tgttgtcatc ctagggccat ctggttgtgg
    20941 gaaaagtacc ctccttcgtt gcctcaacgg cttagaaagt attcaaggtg gagatattct
    21001 tctggatggt cagtctatcg ttgaaaataa aaaagatttt cacctagttc gccaaaagat
    21061 tggcatggtc tttcaaagtt atgaactctt tccccatctg gatgtcttac aaaacctcat
    21121 cctaggccct atcaaagctc aaggaaggta caagaaagaa gtaacggaag aagctttgca
    21181 attactagag cgtgtcggtt tgctggataa acaacatagc tttgcccgtc aattatctgg
    21241 tggacagaag caacgtgttg caattgtccg tgccctccta atgcatccag aaatcatcct
    21301 ttttgacgag gtgactgctt cgctggatcc agaaatggtg cgtgaggtgc tggaacttat
    21361 caatgatttg gcccaagaag gccgtaccat gattttagta acccacgaaa tgcagtttgc
    21421 ccaagccatt actgaccgga ttatcttcct cgaccaaggg aaaatcgctg aagaaggaac
    21481 agctcaagcc ttctttacca atccgcaaac caaacgagcc caggaatttt taaacgtctt
    21541 tgactttagc caattcggct catatctata aaggagattc ttatgaaact attcaaacca
    21601 ctcttaactg ttttagcact tgcctttgcc cttatcttta tcactgcttg tagctcaggt
    21661 ggaaacgctg gttcatcctc tggaaaaaca actgccaaag ctcgcactat cgatgaaatc
    21721 aaaaaaagcg gtgaactgcg aatcgccgtg tttggagata aaaaaccgtt tggctacgtt
    21781 gacaatgatg gttcttacca agactacgct acgatattga actagggaac caactagctc
    21841 aagaccttgg tgtcaaggtt aaatacattt cagtcgatgc tgccaaccgt gcggaatact
    21901 tgatttcaaa caaggtagat attactcttg ctaactttac agtaactgac gaacgtaaga
    21961 aacaagttga ttttgccctt ccatatatga aagtttctct gggtgtcgta tcacctaaga
    22021 ctggtctcat tacagacgtc aaacaacttg aaggtaaaac cttaattgtc acaaaaggaa
    22081 cgactgctga gacttatttt gaaaagaatc atccagaaat caaactccaa aaatacgacc
    22141 aatacagtga ctcttaccaa gctcttcttg acggacgtgg agatgccttt tcaactgaca
    22201 atacggaagt tctagcttgg gcgcttgaaa ataaaggatt tgaagtagga attacttccc
    22261 tcggtgatcc cgataccatt gcggcagcag ttcaaaaagg caaccaagaa ttgctagact
    22321 tcatcaataa agatattgaa aaattaggca aggaaaactt cttccacaag gcctatgaaa
    22381 agacacttca cccaacctac ggtgacgctg ctaaagcaga tgacctggtt gttgaaggtg
    22441 gaaaagttga ttagtcatta actcttaaaa ggaactggat tttaagctcc aatccctttt
    22501 taagatttta cctataacat cctgagtcta tctaagatgt tcaatctgaa cacagtgtac
    22561 atactttatc ttctattgca tatactttat cacataagat acgaatatcc tcttcactat
    22621 gactagcaat caaaattgtt gtcccttttt cactagagag ctttctaaac aatgttctca
    22681 tattttctac acttgattta tccaaggcat tcataggttc atctagtaaa agaatagagg
    22741 gattctccat aattgcttga gcaatcccta gctttttcct catacctagc gaataagttt
    22801 taactttctg gtctttttgc tcatatagac caactatttt cagtgtatca ttgatttcct
    22861 gattaccaac tactcctcgt atgcttgcca aatattgtaa attcttaaag ccactataat
    22921 aatttataaa accaggttct tcaatcaaag ctcccaaatt agctggaatt tttctctcag
    22981 gaacaatatt ttccccattg attaacactt ctccataaga cggactatat aaaccagcta
    23041 ttaatttaaa caatacactt ttccctgagc cattcgcacc agtaattcct ataatttccc
    23101 cctgtttaca actaaagtta aggttttgaa aaacacatgt cttttttaat ttcaactcaa
    23161 tattttttaa tgtaattatt tcattcattc tataaacctc ctcttttgac gagtgaaata
    23221 gaaaatgctt tgaaaaagaa agactaaaaa tagcaactga agaaataaat ctcgtcctat
    23281 atctccattc cctcgattca aaatataaaa tagataatta gttcgatttc ctacaaatag
    23341 accaccaaac acaatcatga gtaaaaagaa actaacgcaa gcaaagttca tttgtacatt
    23401 cgctcctagt gcgtataaca taaagcaaaa caagactaac acacaagcat tcaaagaatc
    23461 aactataaac aaaactccta tatcccagtt tggtaactgt aacataaatt gaatcactag
    23521 aacttttacc atgattaaaa tgagtccata aagaaaacag gtccaaatct gatacctaat
    23581 ccagagctta atagaagaaa atcgaatcac ttccataaag aaatcggatg ctcttctacg
    23641 atatacaact gctagaaaaa atgtatgaat aataaaccaa tgaagcaacc atatagataa
    23701 gactaaaaaa gagttttgac tcagattagg cccttttaaa atttctataa caagtctatc
    23761 atttccttct aaccaatacc ataatcctaa acaaatcaat atccaaaaaa gaacataacc
    23821 ccaaaagtac ttggtataga ttatctgatt caacctccac atgtgactac ttaactttcc
    23881 catttccaac ttttacactc tcctccagtt tctatgaaca gaaaatacag tcaatggaat
    23941 tatgatagat agtaatgtaa gccaataaat caatgcatac ggattggaat ttaccatata
    24001 ctgaaaaatt ggcaataatc caattatccc attgtccaac tgataaatcc agaataagga
    24061 ttctacaaat aaaaaagtaa acagaaatac taagctcatg tttgattttt taaaccaaga
    24121 aaaactgcta atagtaagt
//
LOCUS       DAVHEW010000020        23206 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVHEW010000020 DAVHEW010000000
VERSION     DAVHEW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.020"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            651..2804
                     /locus_tag="VVL53_000775"
     CDS             651..2804
                     /locus_tag="VVL53_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU4182974.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEMGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLIVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2817..4166
                     /locus_tag="VVL53_000776"
     CDS             2817..4166
                     /locus_tag="VVL53_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU4182975.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4336..5043
                     /locus_tag="VVL53_000777"
     CDS             4336..5043
                     /locus_tag="VVL53_000777"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU4182976.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5100..8825
                     /locus_tag="VVL53_000778"
     CDS             5100..8825
                     /locus_tag="VVL53_000778"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU4182977.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNVGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPLVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8918..10360
                     /locus_tag="VVL53_000779"
     CDS             8918..10360
                     /locus_tag="VVL53_000779"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU4182978.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10397..11419
                     /locus_tag="VVL53_000780"
     CDS             10397..11419
                     /locus_tag="VVL53_000780"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU4182979.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVACTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11416..11961
                     /gene="purN"
                     /locus_tag="VVL53_000781"
     CDS             11416..11961
                     /gene="purN"
                     /locus_tag="VVL53_000781"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU4182980.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12045..12554
                     /locus_tag="VVL53_000782"
     CDS             12045..12554
                     /locus_tag="VVL53_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4182981.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12579..14126
                     /gene="purH"
                     /locus_tag="VVL53_000783"
     CDS             12579..14126
                     /gene="purH"
                     /locus_tag="VVL53_000783"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU4182982.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14248..15510
                     /gene="purD"
                     /locus_tag="VVL53_000784"
     CDS             14248..15510
                     /gene="purD"
                     /locus_tag="VVL53_000784"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU4182983.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15913..16401
                     /gene="purE"
                     /locus_tag="VVL53_000785"
     CDS             15913..16401
                     /gene="purE"
                     /locus_tag="VVL53_000785"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU4182984.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16388..17479
                     /gene="purK"
                     /locus_tag="VVL53_000786"
     CDS             16388..17479
                     /gene="purK"
                     /locus_tag="VVL53_000786"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU4182985.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGEPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17489..17716
                     /locus_tag="VVL53_000787"
     CDS             17489..17716
                     /locus_tag="VVL53_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU4182986.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17779..19077
                     /gene="purB"
                     /locus_tag="VVL53_000788"
     CDS             17779..19077
                     /gene="purB"
                     /locus_tag="VVL53_000788"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU4182987.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCNKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19132..23070)
                     /gene="strH"
                     /locus_tag="VVL53_000789"
     CDS             complement(19132..23070)
                     /gene="strH"
                     /locus_tag="VVL53_000789"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU4182988.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAELVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6294 a         4637 c         5578 g         6697 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttacct
      181 attctgacta tggatagatc ataccagagg cggcttagaa atagcaggga cattagaaat
      241 tgaagtaact tttaatagga tgtcgtaagt gttactatca atgatttatt tgtttcaagc
      301 ttgcctaggg tgacagtaaa aaatcaattt cctttcaata gcatattttt agtgggcagt
      361 actcttgttc tgcctatttt tttatccaaa aagtgcagtt gggagggaga taggctcatt
      421 tgggaaggaa gtccagtttt tgtttagtga ttggggtaag atagttgtta tcagatgagt
      481 ttatactctt cgaaaatcaa attcaaacca cgtcaacgtc gccttgccgt atatatgtga
      541 ctgacttcgt cagtcttata tacaacctca aaacagtgtt ttgagcagcc tgcggctagt
      601 ttcctagttt gctctttgat tttcattgag tttaggaaaa ggagatgaat atgaaatttg
      661 ggaaacgtca ctatcgtccg caagtggatc agatggactg cggtgtagct tcattagcca
      721 tggtttttgg ctactatggt agttattatt ttttggctca cttgcgagaa ttggctaaga
      781 cgaccatgga tgggacgacg gctttgggct tggtcaaggt ggcagaggag atgggttttg
      841 agacgcgagc cattaaggcg gatatgacgc tttttgactt gccggatttg acttttcctt
      901 ttgttgccca tgtgcttaag gaagggaaat tgctccacta ctatgtggtg actgggcagg
      961 ataaggatag cattcatatt gccgatccag atcccggggt gaagttgact aaactgccac
     1021 gtgagcgttt tgaggaagaa tggacaggag tgactctttt tatggcacct agtccagact
     1081 ataagcctta taaggaacaa aaaaatggtc tgctctcttt tatccctata ttagtgaagc
     1141 agcgtggctt gattgccaat atcgttttgg caacactctt ggtaaccgtg attaacattg
     1201 tgggttctta ttatctgcag tctatcattg atacctatgt gccagatcag atgcgttcga
     1261 cactagggat tatttctatt gggctagtca tcgtctacat cctccagcaa atcttgtctt
     1321 acgctcagga gtatctcttg cttgttttgg ggcaacgctt gtcgattgac gtgattttgt
     1381 cctatatcaa gcatgttttt cacctcccta tgtccttctt tgcgacacgc aggacagggg
     1441 agatcgtgtc tcgttttaca gatgctaaca gtatcatcga tgcgctggct tcgaccatcc
     1501 tttcgatttt cctagatgtg tcaacggttg tcattatttc ccttgttcta ttttcacaaa
     1561 ataccaatct ctttttcatg actttattgg cgcttcctat ctacacagtg attatctttg
     1621 cctttatgaa gccgtttgaa aagatgaatc gggataccat ggaagccaat gcggttctgt
     1681 cttcttctat cattgaggac atcaacggta ttgagactat caagtccttg accagtgaaa
     1741 gtcagcgtta ccaaaaaatt gacaaggaat ttgtggatta tctgaagaaa tcctttacct
     1801 atagtcgagc agagagtcag caaaaggctc tgaaaaaggt tgcccatctc ttgcttaatg
     1861 tcggcattct ctggatgggg gctgttctgg tcatggatgg caagatgagt ttggggcagt
     1921 tgattaccta taataccttg ctggtttact ttaccaatcc tttggaaaat atcatcaatc
     1981 tgcaaaccaa gcttcagaca gcgcaggttg ccaataaccg tctaaatgaa gtgtatctag
     2041 tagcttctga gtttgaggag aagaaaacag ttgaggattt gagcttgatg aagggagata
     2101 tgaccttcaa gcaggttcat tacaagtatg gctatggtcg agacgtcttg tcggatatca
     2161 atttaatcgt tccccaaggg tctaaggtgg cttttgtggg gatttcaggg tcaggtaaga
     2221 cgactttggc caagatgatg gttaattttt acgacccaag tcaaggggag attagtctgg
     2281 gtggtgtcaa tctcaatcag attgataaaa aagccctgcg ccagtacatc aactatctgc
     2341 ctcaacagcc ctatgtcttt aacggaacga ttttggagaa tcttcttttg ggagccaagg
     2401 aggggacgac acaggaagat atcttacggg cggtcgaatt ggcagagatt cgagaggata
     2461 tcgagcgcat gccactgaat tatcagacag aattgacttc ggatggggca gggatttcag
     2521 gtggtcaacg tcagagaatc gctttggcgc gtgctctctt gacagatgcg ccggtcttga
     2581 ttttggatga ggcgactagc agtttggata ttttgacaga gaagcggatt gtcgataatc
     2641 tcatagcttt ggacaagacc ttgattttca ttgctcaccg cttgactatt gctgagcgga
     2701 cagagaaggt agttgtcttg gatcagggca agattgtcga agaaggaaag catgctgatt
     2761 tgcttgcaca gggtggcttt tacgcccatt tggtcaatag ctagaaagag gagaggatga
     2821 aaccagaatt tttagaaagt gcggagtttt ataatcgtcg ttaccataat ttttccagta
     2881 gtgtgattgt acccatggcc cttctgctcg tgtttttact tggctttgca actgttgcag
     2941 agaaggagat gagtttgtcc actagagcta ctgtcgaacc tagtcgtatc cttgcaaata
     3001 tccagtcaac tagcaacaat cgtattcttg tcaatcattt ggaagaaaat aagctggtta
     3061 agaaggggga tcttttggtt caataccaag aaggggcaga gggtgtccaa gcggagtcct
     3121 atgccagtca gttggacatg ctaaaggatc aaaaaaagca attggagtat ctgcaaaaga
     3181 gcctgcaaga aggggagaac cactttccag aggaggataa gtttggctac caagccacct
     3241 ttcgcgacta catcagtcaa gcaggcagtc ttagggctag tacatcgcaa caaaatgaga
     3301 ccatcgcgtc ccagaatgca gcagctagcc aaacccaagc cgaaatcggc aacctcatca
     3361 gtcaaacaga ggctaaaatt cgcgattacc agacagctaa gtcagctatt gaaacaggtg
     3421 cttccttggc cggtcagaat ctagcctact ctctttacca gtcctacaag tctcagggcg
     3481 aggaaaatcc ccaaactaag gttcaggcag ttgcacaggt tgaagcacag atttctcagt
     3541 tagaatctag tcttgctact taccgtgtcc agtatgcagg ttcaggtacc cagcaagcct
     3601 atgcgtcagg gttaagcagt caattggaat cccttaaatc ccaacacttg gcaaaggttg
     3661 gtcaggaatt gacccttcta gcccagaaaa tcttggaggc agagtcaggt aagaaggtac
     3721 agggaaatct tttagacaag gggaaaatta cggcaagtga ggatggggtg cttcatctta
     3781 atcctgagac cagtgattct agcatggttg cagaaggtgc cctactagcc caactttatc
     3841 catctttgga aagagaaggg aaagccaaac tcacagccta tctaagttca aaagatgtag
     3901 caagaatcaa ggtcggtgat tctgttcgct atactacgac tcatgatgcc gggaatcaac
     3961 tttttctaga ttctactatt acaagtattg atgcgacagc tactaagact gagaaaggga
     4021 atttctttaa aatcgaggcg gagactaatc taacttcgga gcaggctgaa aaacttaggt
     4081 acggggtgga aggccgtcta cagatgatta cgggcaagaa aagttaccta cgttattatt
     4141 tggatcaatt tttgaacaaa gagtaatgtt cgtgttttta gagttaaata atttttaaac
     4201 tgtgagaaag attcttcttg cagttttttc tttacaattt ttgaaaaaca tctgctattt
     4261 attcggttga attcttgtgt tttttggttt tttgtggtaa aatgtgctca agtaatacga
     4321 aaggcgaact ttaaaatgtc aaaacaattg atctattcgg gaaaagctaa agatatctat
     4381 acaactgagg atgaaaatct tattatttca acttacaagg accaggcgac tgctttcaac
     4441 ggtgtcaaga aggagcagat tgcaggtaag ggagtcttga ataatcagat ttcatctttt
     4501 atttttgaga aattaaatgc ggctggtgtg gcgactcact ttgtggagaa actttcagac
     4561 acggaacaac tcaataaaaa ggttaagatt attcctttgg aagtcgtgct ccgcaactat
     4621 actgctggtt ccttttcaaa acgttttggt gtggatgagg gaatcgcctt ggagactccg
     4681 attgtcgaat tttactacaa aaatgatgat ttggatgatc catttatcaa tgatgagcat
     4741 gtgaaattcc tacagattgc gggtgaccag cagattgcct acttgaagga agaaacgcgt
     4801 cgtatcaatg aactattgaa agtctggttt gctgagattg ggcttaaatt gattgacttt
     4861 aagctagagt tcggttttga caaggatggc aagattatct tggcagacga attttcacca
     4921 gataactgcc gcttgtggga cgctgatggc aaccacatgg ataaggatgt tttccgtaga
     4981 ggattgggag aactaaccga cgtttatgag attgtttggg aaaagttgca gggtttaaaa
     5041 taacaacctc aaggctgttt gggaatattg caagagctga aataaaggaa taagaattga
     5101 tggataaacg tatttttgtt gaaaaaaagg ctgattttca ggtcaagtca gagagtttgg
     5161 ttagagagct ccagcacaac gtgggactgt caagcttgaa aagtattcgt attgtgcaag
     5221 tatatgatgt atttgacttg gctgaggact tgtttgcacc tgcagagaag cacattttct
     5281 ctgagcaggg aactgaccat gttttagatg aagtatctgt gcaggcggat cttgctaact
     5341 atgctttctt tgccattgaa agtctgccag ggcagtttga ccagcgtgca gcttcgtcac
     5401 aggaagcctt gcttttgttg ggaagttcga gtgacgtgac agtcaacaca gcccaacttt
     5461 acttggtgaa taaagatatt gatgcgactg agttggaagc tgtcaaaaac tacctgctca
     5521 atccagttga ttctcgtttc aaggatatca cgacagggat tgccaagcag gagttttcag
     5581 agtcagacaa gaccattcca aaattgactt tctttgaaag ctatacagca gaagactttg
     5641 ctcgctacaa ggccgagcaa gggatggcca tggaagtgga tgatttgctc tttatccaag
     5701 actactttaa gtcaatcggg cgcgtgccaa ctgagactga actcaaggtt ttggacactt
     5761 actggtctga ccactgccgt catacgactt ttgagacaga gttgaaacac atcgactttt
     5821 cagcttctaa atttcaaaag caattgcagt caacctatga caagtatatt gccatgcgcg
     5881 aggagttagg tcggtctgaa aaaccacaaa ccttgatgga tatggcgact attttcggtc
     5941 gttatgagcg tgctaatgga cgattggatg atatggaagt ctctgacgaa atcaatgcct
     6001 gctcagttga aattgaagtg gatgttgatg gtgtcaagga accttggctc ctcatgttta
     6061 aaaatgaaac ccacaaccat ccaacagaaa ttgagccatt tggtggagcg gctacctgta
     6121 tcggtggagc tattcgtgat ccgttgtcag gccgttccta tgtttaccaa gccatgcgta
     6181 tttcaggtgc tggtgatatt acagcaccga tttcggaaac tcgtgctggg aaattgccac
     6241 aacaagtcat ttctaaaaca gcagctcatg gttattcttc atatggtaac cagattgggc
     6301 ttgccacaac ctacgttcgt gaatacttcc acccaggctt tgtagctaaa cgtatggaac
     6361 ttggtgccgt tgttggtgcg gctcccaagg gcaatgttgt ccgtgaaaaa cctgaagcag
     6421 gtgatgtgat catccttctc ggaggcaaaa caggtcgtga tggtgtcggt ggtgcgacgg
     6481 gctcttctaa ggttcaaaca gttgagtctg tagagactgc tggtgctgag gttcaaaaag
     6541 gaaatgccat cgaagaacgc aagattcagc gcctcttccg taatggcaat gtcactcgtc
     6601 tgatcaagaa gtccaatgac tttggggcag gcggcgtctg tgtggctatc ggtgaattgg
     6661 cagacggtct tgaaatcgac ctcaacaagg tgcctcttaa ataccagggc ttgaatggta
     6721 cagaaattgc catctctgaa tcacaagaac ggatggcggt cgtggttcgt cctgaagatg
     6781 tggatgcctt cgttgccgaa tgtaacaaag aaaatattga tgctgttgtg gtggcgacag
     6841 taactgaaaa accaaatctt gtcatgcact ggaatggtga gacaatcgtt gacttggagc
     6901 gtcgtttcct tgacaccaat ggtgtgcgcg tggttgtcga tgccaaagtt gtggacaagg
     6961 atgtcaaact cccagaagag cgtcaaacat ctgctgaaac actggaatca gataccctta
     7021 cggttctatc tgacctcaac catgcaagtc aaaaaggatt acagactgtc tttgactgct
     7081 ctgttggacg ctcaacggtt aatcacccac ttggtggtcg ttaccaactc acaccaactg
     7141 aggcatctgt gcagaaattg ccagttcaac acggtgtgac tcatactgcg tcggtcattg
     7201 ctcaaggttt caacccatat gtagctgaat ggtctccata ccacggtgct gcttacgcgg
     7261 ttatcgaagc aactgctcgt ttggtggctg ctggtgccaa ctggtctaag gctcgtttct
     7321 cttaccaaga gtatttcgag cgtatggata aacaagcaga gcgttttggt cagccagtag
     7381 ccgcccttct aggctctatt gaagctcaga ttcaacttgg cttgccatct atcggtggta
     7441 aggactccat gtctggtacc tttgaagaat tgaccgttcc gccaaccttg gttgcctttg
     7501 gggtgacgac ggcagatagc cgtaaggtgc tctctccaga atttaaagct gttggggaaa
     7561 atatctacta catcccaggt caagccctct ctgcagagat tgattttgac ttgattaaga
     7621 aaaattttgc tcagtttgaa gcaagccaag ctgaccataa agtgacatct gcatcagctg
     7681 tcaaatacgg tggtgtagtt gaaagtttgg ctcttgctac ctttggaaac tatattggtg
     7741 cagaggtgac cttgcctgaa cttaaaacag ctttgacagc tcaattaggc ggctttgtct
     7801 tcacatctcc tgaagaaatt gctggagtag agaagattgg acaaacgaaa gcagacttta
     7861 cactgactgt caacggtgtg aagctagatg gacacaagct tgacagtgca tttcaaggga
     7921 cattggaaga agtttaccca acagaattta cccaagcgaa agaactagaa gaagtaccag
     7981 ctgtggcatc agatgttgtg attaaagcca aagaaaaggt tgaaaaacct ctggtttaca
     8041 tcccagtctt tccaggaacc aactcagaat atgattcagc taaggccttc gaaaaagaag
     8101 gtgcagaggt caatttggtg ccattcgtga ccttgaatga agaagctatt gtcaagtcag
     8161 ttgaaactat ggttgacaat atcgacaaga ctaatattct cttctttgcc ggtggattct
     8221 cggctgcgga tgaaccagat ggttcagcta agtttatcgt caatatcctg cttaatgaaa
     8281 aagtgcgtgt ggctattgat agctttatcg cccgtggtgg cttgattatc ggtatttgta
     8341 atggattcca agccttagtc aaatcgggtc tcctaccata cggaaacttt gaagatgcta
     8401 acagtactag tccaaccctc ttctacaatg atgccaacca acacgtggcc aagatggtgg
     8461 aaactcgcat tgccaatacc aactcaccat ggttggctgg tgtgcaagtg ggcgatatcc
     8521 acgctattcc tgtttcgcac ggtgaaggga agtttgtcgt gacggctgag gaatttgcag
     8581 agctccgtga caatggacaa attttcagcc aatacgttga ctttaacggt aaaccaagta
     8641 tggattctaa gtacaatccg aatggttctg tccatgccat cgaaggaatt accagcaaga
     8701 atggtcaaat catcggtaag atgggccact cagaacgtta tgaggatggt cttttccaaa
     8761 atatcccagg caataaagac caacacctgt tcgcatcagc ggttaaacat ttcactggaa
     8821 aataagactt acagattttc taatagatag tatcagtaat gtaaaagtca tgtaaatcta
     8881 gctcttgatg attacaaatg aaaattaggt ataaaaaatg acatacgaag taaaatctct
     8941 taatgaagaa tgtggtgttt tcggtatttg gggacatcca gatgctgcta agttgaccta
     9001 ttttggactc cacagtcttc aacaccgtgg tcaggagggg gcaggaattc tctccaatga
     9061 tcaaggacaa ctgaagcgcc atcgtgacat ggggctttta tcagaagttt tcagaaatcc
     9121 agccaatttg gataaattga caggagctgg tgcgattggg catgtgcgtt atgcgactgc
     9181 tggcgaagct tctgttgata acatccaacc cttcctcttc cgttttcacg atatgcagtt
     9241 tggcttggct cataatggaa atctgaccaa tgcagcctct ctcaagaaag aactggaaca
     9301 aagaggagca attttcagcg cgacttcgga ctctgaaatc ttggctcacc tcattcgtcg
     9361 cagtcataat cctagcctga tgggcaaaat caaggaagcg ctcagccttg tcaaaggtgg
     9421 ttttgcctat atcttgctgt ttgaggacaa gttgattgcg gctcttgacc ctaatggctt
     9481 tcgtccgctt tctatcggga aaatggccaa cggagcggtg gttgtttcct ctgaaacctg
     9541 tgcttttgag gtcattggtg ccgagtggat tcgtgatttg aagccaggtg agattgtgat
     9601 cattgatgac gagggcattc agtatgacag ctatacagat gatacccagt tggcgatttg
     9661 ttctatggag tatatctact ttgcccgccc tgattctaat atccacggtg tcaatgtcca
     9721 tacggcacgt aagagaatgg gagcgcaatt ggcgcgagaa tttaagcatg aggcagatat
     9781 tgtagttggt gtgcccaatt cttccctaag cgcggctatg ggatttgcgg aagaatcagg
     9841 cttaccaaat gaaatgggtc tgatcaaaaa ccaatacacc cagcgaactt ttatccaacc
     9901 gactcaagaa ttgcgggagc aaggagtgcg gatgaaactg tctgctgttt cgggtgttgt
     9961 caaaggcaaa cgtgtggtca tggtggatga ttccattgta cgtggaacaa cctctcgtcg
    10021 tatcgttcag ctcttgaaag aagcgggtgc gactgaggtt cacgttgcca ttggaagtcc
    10081 tgcactagcg tatccatgtt tctacgggat tgatatccag acccgtcagg agctgattgc
    10141 agccaatcat acggtcgaag aaactcgcca aatcattggt gcggacagta tgacttatct
    10201 ttcaattgat ggcttgattg agtcgattgg tatcgaaaca gatgcgccga acggtggtct
    10261 ctgtgtcgct tactttgacg gtgactaccc aacgcctctc tatgactacg aagaagacta
    10321 tcgtagaagt ttggaagaaa agaccagttt ttacaagtag gcgacagatt ctccattaaa
    10381 gaaaaggaaa aaacaaatga caaataaaaa tgcatatgcc cagtcgggtg tggatgttga
    10441 agcgggttat gaagttgttg aacggattaa aaagcacgtg gcctgtacgg agcgtgcagg
    10501 tgtcatggga gctcttggtg gctttggtgg tatgtttgac ctttccaaga ctggggttaa
    10561 agaacccgtc ttgatttcag ggactgacgg tgtcggaacc aagctcatgt tggctatcaa
    10621 gtacgacaag cacgatacca tcgggcagga ctgtgtggcc atgtgtgtca acgacatcat
    10681 tgctgcaggt gcggaacccc tctattttct cgactacgta gcgacaggga agaatgaacc
    10741 agctaagcta gaacaagtgg ttgctggtgt ggcagaaggt tgtgtgcagg ctggtgctgc
    10801 cctcatcggt ggggaaacgg ctgaaatgcc gggcatgtac ggcgaagacg actatgactt
    10861 ggctggtttt gcggtcggtg tggctgaaaa atctcaaatc attgacggtt caaaggtggt
    10921 agagggagat gttcttctcg gacttgtttc aagtgggatt cactcaaatg gttactcttt
    10981 ggttcgtcgt gtctttgcgg attacacagg tgaggaagtc ctaccagaat tggaaggcaa
    11041 gaaacttaag gaagttctac ttgagccgac tcgtatctat gtcaaggctg tcttgccgct
    11101 catcaaagaa gagttggtca acggcattgc ccacatcaca ggtggtggct ttatcgaaaa
    11161 tgtccctcgt atgtttgcag atgacctagc tgctgaaatt gatgaaagca aagttccagt
    11221 gcttccaatt ttcaaagccc ttgaaaaata tggtcagatt aaacacgaag aaatgtttga
    11281 aatcttcaat atgggtgtgg gacttatgtt ggcggtcagc cctgaaaatg tagagcgtgt
    11341 aaaagaattg ttggatgaag cagtctatga aattggtcgc atcgtcaaga aagaaaacga
    11401 aagtgtcatt atcaaatgaa aaaaatagcg gtttttgcct ctggtaatgg ctcaaatttt
    11461 caggtgattg ccgaagaatt tccagtggag tttgtctttt cagaccatcg tgatgcctat
    11521 gtgcttgagc gtgcaaagca gctcggcgtt ctgtcctatg cttttgaact caaggagttt
    11581 gagagcaaga cagactacga agcagccctt gtcgaactct tggaagaaca ccagattgac
    11641 ttggtttgcc tagcaggcta catgaaaatc gttggaccaa ccttattgtc ggcttatgaa
    11701 ggtcggattg tcaacattca tccagcctac ttgccagaat ttccaggagc tcatgggatt
    11761 gaggatgctt ggaatgctgg cgtgggtcag tctggtgtga ccattcactg ggtggattcg
    11821 ggtgtggata caggccaggt catcaaacag gttcgtgtgc cacgactagc tgatgatacc
    11881 attgacagat ttgaagctcg catccatgaa gcagagtaca ggttgtatcc ggaagtagtg
    11941 aaggctctat ttacagattg actttttgat gattcatatg atatctttga ttttaaattg
    12001 gagtcagtgt ttgttgaaga cggcttcaaa cggaggtatt tgtaatgtta gaatctaaaa
    12061 aaacaactcg atatgtattt tatgtctatc tgatgttatt aacttgggga atcttattta
    12121 agtttgaaac aaatcctgaa tttatagcat ttttcttagc tccaaggtat atcaattgga
    12181 ttccattttc agaaccacta atagtcgatg gaaaaattgt ttttgctgaa atgttattta
    12241 atctgatttc ctttattcca ttaggtgttt gtttcccttt gataaaaact aatttatcta
    12301 gtttaagaat agtcgggaca ggtttcttga ttagtttatt gtttgagtgc ttacagtata
    12361 ttttagcaat aggtataaca gatataacgg atttgacttt aaatacgcta ggtgtctgtg
    12421 taggcttact gatttatcaa atttttataa gagtgttcaa atcacagact agaaaatgga
    12481 tcaatatctt aggtatgctt agccttggtt ttgcttatct tgttttactg ttactgcatt
    12541 taattggtgt ttaactaatg attaaaaagg agaatataat gactaaacgc gtcttaatca
    12601 gcgtctcaga caaagcgggc attgttgaat ttgcccaaga actaaaaaaa cttggttggg
    12661 agattatctc gacaggtgga actaaggttg cccttgataa tgctggggtg gagaccattg
    12721 cgatcgatga tgtgacgggc ttcccagaga tgatggatgg ccgtgttaag actcttcatc
    12781 caaatatcca cgggggtctt ctcgctcgtc gtgatttgga tagccacttg gaagcagcca
    12841 atgagaacca aattgagctt attgaccttg tagtggtcaa cctttatcct ttcaaggaaa
    12901 cgattctcaa accagacgtg acgtacgctg atgctgtgga gaatattgat attggtgggc
    12961 catctatgct tcgttcagca gcgaagaatc atgccagcgt gacggttgtg gtagaccctg
    13021 ctgactatac agtggttttg gacgagttgt cagccaatgg tgaaacgact tacgaaacgc
    13081 gtcaacgttt ggcagccaag gttttccgtc acacagcggc ttatgacgct ttgattgcag
    13141 aatacttcac agctcaagtg ggcgaaagca aacctgaaaa gttgaccttg acctatgacc
    13201 taaaacaagc tatgcgttat ggggaaaatc cccagcagga tgcggacttc taccagaaag
    13261 ctttgccgac tgattactcc attgcatcag ccaaacagct taacggtaag gaattgtcat
    13321 tcaacaatat ccgcgatgca gatgctgcca ttcgtattat ccgtgacttc aaagaccgtc
    13381 caaccgttgt ggctctcaag catatgaatc catgtggtat cggccaggcg gatgacatcg
    13441 agacggcttg ggattatgct tatgagtctg acccagtgtc tatctttggt ggtatcgctg
    13501 ttcttaaccg tgaggtggat gctgcgacag ctgagaagat gcacggcgtt ttcctcgaaa
    13561 tcatcattgc accaagctat acggatgaag cgctagccat tttgatcaat aaaaagaaaa
    13621 acttgcgtat ccttgccttg ccatttaatg ctcaagaggc tagcgaagtg gaagcagaat
    13681 acacaggtgt agtcggtgga cttctcgtgc aaaatcaaga cgtggtcaag gaaagcccag
    13741 ctgactggca agtggtgacc aaacgtcagc caaccgagac agaagcgact gctcttgagt
    13801 tcgcttggaa ggctatcaag tacgtcaaat caaatggtat tatcgtgacc aacgaccaca
    13861 tgacacttgg tgttggtcca ggtcaaacca accgtgtggc ttctgttcgc cttgccattg
    13921 accaagccaa agatcgtctg gacggggcgg tccttgcttc agatgccttc ttcccatttg
    13981 cggataacgt ggaagaaatc gccaaagcag gaattaaggc catcatccag cccggtggct
    14041 ctgtccgtga ccaagaatcc atcgaagcag cggataaata cggcttgact atggtcttta
    14101 caggtgtgag acattttaga cattaagaag ataaaaggga agaaaacagt ttctttcctt
    14161 ttttggctta aaatgctaac tgaaacaaga ttaaaacgaa cttttttgat ataatgttgg
    14221 taaataattc gcaaaagagg ttgaggaatg aaactgcttg ttgtcggttc tggtggtcgt
    14281 gagcatgcga ttgctaaaaa gttacttgaa tcaaaagacg tggaaaaagt ctttgtagct
    14341 cctgggaatg atgggatgac tctggatggt ttggaattgg taaatatctc tatttccgaa
    14401 cattctaaat tgattgactt cgcaaagacc aatgatgttg cttggacctt tatcggtcca
    14461 gatgatgccc ttgctgctgg tatcgtggat gattttaacc aagctggact taaggccttt
    14521 ggtccgacta gggctgcagc ggagctggag tggtccaagg atttcgccaa ggaaatcatg
    14581 gtcaaatacg gcgttccgac agcaacatat ggcacatttt cagatttcga ggaagccaag
    14641 gcctatatcg aaaagcatgg tgcgcctatc gtagtcaagg cggatggctt ggcacttggg
    14701 aagggtgtcg tcgttgctga gacggttgag caagcggtcg aagccgctca tgagatgctt
    14761 ttggacaata aatttggtga ctcaggtgcg cgcgtggtta ttgaggaatt ccttgaagga
    14821 gaggaatttt cactctttgc ctttgtcaat ggtgataagt tctacatcat gccaacggct
    14881 caggaccaca aacgtgccta tgatggcgac aaagggccta acacgggtgg tatgggtgcc
    14941 tatgcgccag tcccacactt accacagagt gtagttgata cagcggttga caccattgtc
    15001 aagccagttc tagaaggggt gattaaagaa ggtcgccctt atctgggagt tctttacgca
    15061 gggcttatcc tgacagctga tggaccgaaa gtcattgagt tcaacgctcg gttcggagat
    15121 ccagaaactc agattatctt gcctcgcttg acctctgact ttgctcaaaa tatcacagat
    15181 atcctggata gcaaggagcc aaatatcatg tggacggaca agggtgtgac tctgggtgtg
    15241 gttgtcgcat ccaagggcta cccgctagac tatgaaaggg gcgttgagtt gccagcaaag
    15301 acagaaggcg atgtcatcac ctactatgca ggggctaagt ttgcggaaaa tagcagagca
    15361 ctgctctcaa acggcggacg agtttatatg ctcgttacca cagcagatac cgtcaaagaa
    15421 gcccaagcca gcatctacca agaactatcc caacaaaaaa tagaaggact cttctaccga
    15481 acagatatcg gaagcaaggc aattaagtaa agatataaga ataacgcgcc gtagtcgcca
    15541 aacacgataa tggtcgtcgt ggtgaaaaga ccagaacagt gaatgttctg gtcaggggga
    15601 aacttggaga ccttaggctc aaagtttagg aatgaaaccg aaggtttgct tccgcctcca
    15661 tcacctaaga ccattatcaa aaagaaaaat aaaaattcac aaaatacgtt aatgatcgta
    15721 tggtttgcga gcgttagcga gctaatatag aacaatcacc gccgttgtga aagaacgatt
    15781 ggatgataat ccaatcgttc agggaaattg gaagaccttg ggtttccaat ttaggcatga
    15841 gacacctttg gtggctgctg ccgtccctca caagctaagg tgattgttga aaaagaggaa
    15901 aaaggagaag aaatgaaacc agtaatttcc atcatcatgg gctcaaaatc cgactgggca
    15961 accatgcaaa aaacagcaga agtcctagac cgcttcggtg tagcctacga aaagaaagtt
    16021 gtttccgcac accgtacacc agacctcatg ttcaaacatg cagaagaagc ccgtagtcgt
    16081 ggcatcaaga tcatcatcgc aggtgctggt ggcgcagcgc atttgccagg catggtagct
    16141 gccaaaacaa cccttccagt cattggtgtg ccagtcaagt ctcgtgctct tagtggagtg
    16201 gattcactct attctatcgt tcagatgccg ggtggggtgc ctgttgcgac catggctatc
    16261 ggtgaagctg gagcgactaa cgcagctctc tttgccctcc gtctcctctc tgtagaagat
    16321 aagtccattg cggatgcact tgccaacttt gctgaagaac aaggaaaaat cgcagaggag
    16381 tcgtcaaatg agctcatcta aaacaatcgg aattatcggt ggcggtcaac tgggtcagat
    16441 gatggccatt tctgctatct acatgggcca caaggttatc gcgctggatc ctgcggcgga
    16501 ttgcccggcc tctcgcgtgg cggaaatcat tgtggcacct tataacgatg tggacgccct
    16561 ccgtcagttg gcagaccgtt gcgatgtcct cacttacgag tttgaaaatg tcgacgctga
    16621 cggtttggat gccgttatca aggatggaca actccctcaa ggaacagatc tgctccgcat
    16681 ttcgcaaaat cgtatttttg aaaaggactt tttgtcaaac aaggctcaag tcactgtggc
    16741 accctacaag gtcgtgactt ctagcctaga cttggcagat atcgacttgt cgaaaaacta
    16801 tgtcctcaaa actgcgactg gtggctacga tggtcatgga caaaaggtta ttcgttcaga
    16861 agcagacttg gaagcagcct atgcgctagc agactcagca gactgcgtct tggaagaatt
    16921 tgtcaacttt gaccttgaga tttctgtcat cgtgtcagga aatggcaagg aggtgacgtt
    16981 tttcccagtt caggaaaata tccaccgcaa caatatcctg tctaagacca tcgtaccagc
    17041 ccgcatttct gaaagtctag cagacaaggc taaagctatg gcagtgcgaa tcgcagaaca
    17101 actcaacttg tctggaactc tctgtgtgga aatgtttgcg actgatgatg acgttattgt
    17161 caatgaaatc gccccacgac cacataactc tgggcactat tctattgaag cctgtgattt
    17221 ctctcagttt gacacccata ttctcggtgt tctcggagaa ccattaccag tcatcaaact
    17281 ccatgcgcca gccgttatgc ttaatgtcct cggtcagcat gtcgaggctg ctgagcaata
    17341 tgtgacagaa aattcaagcg cccacctcca catgtatggt aaaatagaag cgaagcacaa
    17401 ccgcaagatg ggacatgtga ccttgtttag tgatgtaccg gatagtgtgg atgagcttgg
    17461 ggaagggatt gatttttagg acaagtctat gatacaaatt atcgttaata catttattga
    17521 aaaggataag actggagcag ttgttgaagt gttgtatgcc agtgctgacc aagataaggt
    17581 acaagctaaa tatgaagaac tagctgcaca ataccccgaa aattatttag ctatctatga
    17641 tgtaccgctg gatacggatt tgaatacact agatcattac ccgtctgtgt ttattgaaaa
    17701 agaggagttt gagtagaaat cttggtttac ctagatagct tattcccaac agcttaagaa
    17761 gaaaggaaaa attaacacat gatcaaccgt tactctcgcc ctgagatggc gaatatttgg
    17821 agtgaagaaa ataaataccg tgcttggctt gaggtggaaa tcctctctga cgaggcatgg
    17881 gctgagttgg gggaaatccc taaggaagat gtggctttga ttcgcaagaa ggcggacttt
    17941 gacatcgacc gtattttgga aattgagcag gagacgcgcc acgatgtggt ggctttcacg
    18001 cgtgcggttt ctgagactct tggtgaagag cgcaagtggg ttcactatgg gttaacttct
    18061 accgacgtgg tggatactgc ttatggttac ctctacaagc aggccaacga catcatccgt
    18121 cgtgaccttg aaaacttcac taatatcatc gctgacaagg ccaaggagca caagttcacc
    18181 atcatgatgg ggcgtactca tggtgtgcac gctgagccga caacttttgg tcttaaattg
    18241 gcaacttggt atagcgaaat gaaacgcaat atcgagcgtt tcgagcatgc ggctgctggt
    18301 gtagaagctg gtaagatttc tggtgcggtt gggaactttg ccaatatccc accatttgta
    18361 gaggagtatg tctgcaacaa gcttggtatc cgtgctcaag aaatctctac acaagtcctt
    18421 cctcgtgacc ttcacgctga gtactttgca gttcttgcca gcattgcgac ttcaatcgaa
    18481 cgtatggcga ctgagattcg tggtctacaa aaatctgagc aacgcgaagt agaagagttc
    18541 tttgctaaag ggcaaaaagg gtcttcagca atgcctcaca aacgcaaccc aatcggttct
    18601 gaaaatatga ctggtctggc gcgtgtcatt cgtggtcaca tgattacggc ttatgaaaac
    18661 gtcgctctct ggcacgaacg cgatatttcc cactcatcag ctgagcgtat catcacacca
    18721 gatacgacca ttttgattga ctacatgctc aaccgttttg gaaatatcgt caagaacttg
    18781 acagtcttcc cagaaaatat gatccgaaac atgaactcga cttttggtct tatctttagc
    18841 caacgggcta tgttgacatt gattgaaaaa ggcatgaccc gtgagcaagc ctatgacttg
    18901 gtgcaaccaa aaacagccta ctcttgggac aaccaagtag actttaaacc acttcttgag
    18961 gcagattcag aagtaacatc acgtctcact caagaagaaa tcgatgaaat cttcaaccca
    19021 gtttattaca ccaaacgagt ggatgatatc tttgaacgtc ttggactagg tgattaatta
    19081 aaaaataaac agcgagcttc aatctcgctg tttatttttt atcgaaaaga cttagtcttc
    19141 ttttctttta gtgagtccat aggctgctag tgtggacatg agtcctgcga ctactagttc
    19201 tgcagaatcg tgagttcctg tttcaggaag ttttttctct gttaccacag gagctggatc
    19261 ttgaggaaga gctttgcttt cctcagcagg agcagttgat ggagctggtt ggcttgggat
    19321 ttctagtttt ggtttttctt cagcaatagc ggcttgtccg ttttcatcgc ctacatgtgt
    19381 taccatagtt ccgacttcga ctatttgagt aacggcttcc tgtgctacga cactatttac
    19441 aagtgttttc acttccttac catcggcaga agtgctcaca gagtagaagt tgctacgatg
    19501 tccattgacg cccttagtaa tgacttgtgt ttttcctttg agtaagagtg gattttcacg
    19561 agtcactgtg gtaaatggaa tttcttcttt ttggatatcc agtctaggtt ttacctcagt
    19621 agttggtgca agaccacttt catcaccctt gtgagttaca ggagcgccaa cttcaaccac
    19681 ttggtttata acttctttgg ttacctggct atcaaggact gtttctgttg tttttccatt
    19741 ttcagtgagt acagagatgt aatgagttcg ttcacctttg actcctgctg tgataatatt
    19801 ttcctgacca gctgggaggt taggattttc tttcttgata acttcaaatg gaatttcttc
    19861 agttcttgtg atgagttctg gtctggtttc aacattggca gccacttcat tttcatccag
    19921 gcttcctgaa tgagttgcag ctggtttgag gccttgaaga gcggctttta ggttggctac
    19981 aagtgtgtca agctcagctt gtttattacg gttgaggttg taatttagag ctgttttagc
    20041 tgcgtcaagg gcctcaagac tttctttact atatccttct aagtttgtag gaattttagc
    20101 taattcttcg cggagagcat tataattagc acggaagtag tctttgttgt ggtctgcaaa
    20161 ggcagtcatg agttcaaaga tttcctcttc cttgtactca gcgcttggtc tatctgccca
    20221 gattgaaagc atacttccga ctgttggaag atctacttca ggatatttgg tagaagctag
    20281 ttgattgaat ggtgtttttc cagtattctc aatagctttc ttgaggaaac caccaccatc
    20341 ttctggtttt tgaccaagaa tgtagtacca gtcaccgttg gtattcaaga atttatagcc
    20401 tttgcttgct aggtattgag gtgatgcgag gttatatccc caccagcctt tagaccagta
    20461 agaaatcaag acatctttgt caaactgaac atcgtccttg tcttcatagt agaagccatc
    20521 gttgaaggcc attggttgaa gccctctttc tttggccata gctgcgaggg tgttggcata
    20581 ttcggcaaat ttgccataga gttgatacca cttgaggtag taccagcctt gggcactagt
    20641 cgcatcgttg gcgtattcgt cagtaccaaa gttgaaaatc tttgttttac ctgcaaagaa
    20701 gtccatgtat ttaccgatga gggcttttac aaagttcatc gcttcttcgt ttttcaagtc
    20761 catagttgtt tttgaaactt tatcaaagtg ggcttgagga tttttaatac ctaatttttc
    20821 catggcaacc agcatagcat ccatgtgacc tggactgtta atagctggga tgagaccgat
    20881 gtccttagat ttagcgtatt caattagctc tgttacttct gcctgtgtta gtgcagtacc
    20941 gtttggatcg tcgtagtaag ctttagttcc ttcgataata gcttttttaa cgtcatcact
    21001 agcataggtt tttccgttgg cagtaatggt catatcatcg agtagaaagc gaagtccgtc
    21061 atttcctaga aggagatgga catcagaata tccgagctca ctggccttgt ctacgatgcg
    21121 tttgagctgg ttcagagtaa agtatttgcg tccagcatcg attgagatta ccttgttttt
    21181 ggcaagtttt tcaacctcac gtttagcttc ttcttctttt agagcttcag gcgtgagggt
    21241 caagttgttg acagtttctt gaagtttagc aatggcttga tcaatcgtat cttgttgggc
    21301 acggctaagg ttgctatcga gagagcgaat agctttttca gcttctttta cggccgtgac
    21361 gctttctgca gtataacggt tcaggtcttt tggtacctcg ttaagtgctt gctctgcaga
    21421 ttcataatca gctgcgaagt attcagcgtt ggcatttgca aaatgacgca tgagtttgaa
    21481 gaggcgtgat ggtgaataac gtgcagatgg agtgtcagcc caagcagcta ccataccacc
    21541 gatgattggg atatcagctc cttctgtttt tggtacagaa gtgattggtg tgtttttaat
    21601 accattgagc ccctgatcga gattgtacca gccttggcca tcagcgtttc gtccaagaac
    21661 gtagtaccaa gcatcattgg tattaaggat ttggtgacct ttttcagcta gtagtttaga
    21721 agaagcgaca tcgtagcctc cccaaccacc agtccacata gaaacgatga tgtctttgtc
    21781 aaaactacca aagcttgtgt cgctattgta gtagataccg tcgttaaaag ccattggttt
    21841 gagaccgtgc gattttacaa tacgagcgag gtcattggcg taggcaataa atttttcata
    21901 gccttttaca gggtagcctt cgtttggata gtatttatca gcttgaagca cactccaacc
    21961 tttagcatct gtcgcatcat tggcatattc atcaagtccg atgttgaaga tttcagtctt
    22021 tttcgcgaaa taagcagcat acttgtcgat aagggctttt gtaaaagcga cagcttgttc
    22081 gttgtcaaga tcgacagtac gggctgattt cttcccaaaa tagctaaagt tagggttttg
    22141 gattcccaat tctttcatgg cattgagaat cgcatccatg tgtccaggac tatttactgt
    22201 cggaatgaga ccgatacctt tatctttggc atagttaatc agatctgtca tttgactttc
    22261 tgttaagtga ttgccgtttg gatcgttgta ataatcattt gtaccttttt caatggcgcg
    22321 tttgacatcg tcactggcat aggtcttgcc gttagctgtg atgctcatat cgtccaacat
    22381 gaaacggagt ccatcatttc cgactaatag gtgtaaatca gtgtagccat aatgtttcgc
    22441 tttatcgatg atttccttga gttgttctgg tgagaaatat ttacgtccag catcaataga
    22501 aacaattttc tttttcgcta gtttttcatt tacagttgca gcacgttcct ttcctgcctc
    22561 tgtttccggt ttgtcagcct ctgctttcgc ttcatctttt ttagctggtt tatccttgtc
    22621 agtcttgtct gtatttgact ctttagaatc aacctctttc gcttcttcct ttttagggct
    22681 agcttcttct gcctttttat tagcagtttc tttttcagca gaagttggag ttaccacttc
    22741 tgctttatca ctaggagttg aactaacttc ctcttgtggt ttttcttctg tttttggaag
    22801 actagctacc ttatcagtag ctggagtttc tgtttctaca gtttttggag cttctggttg
    22861 aagcactgct ttaggtgttt cctcagtccg attttcggat gattgagggg aatcagaaac
    22921 cgtatggatg gtcggttggt tttctgtagt agtaggagta actccatcgg ctgcaacagt
    22981 ctgtgcttgg aaggcaaatc caattagaac agaagctgct cctacagcgt atttacgaat
    23041 agaaaaacgc tgttgttttt catgtttcat tgccaaacct cctgattgca ttgttatatt
    23101 gatagcgatt atataaatca acgcctttat tttatttctt atattaacga gagtcaagag
    23161 gagatgacaa aaaactataa taagtataaa aaaatataaa atttaa
//
LOCUS       DAVHEW010000021        22714 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVHEW010000021 DAVHEW010000000
VERSION     DAVHEW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.021"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(60..509)
                     /locus_tag="VVL53_000790"
     CDS             complement(60..509)
                     /locus_tag="VVL53_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU4182989.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(511..1269)
                     /locus_tag="VVL53_000791"
     CDS             complement(511..1269)
                     /locus_tag="VVL53_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU4182990.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VVL53_000792"
     CDS             complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VVL53_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU4182991.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3360..4100)
                     /locus_tag="VVL53_000793"
     CDS             complement(3360..4100)
                     /locus_tag="VVL53_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU4182992.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VVL53_000794"
     CDS             complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VVL53_000794"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU4182993.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5421..6356)
                     /locus_tag="VVL53_000795"
     CDS             complement(5421..6356)
                     /locus_tag="VVL53_000795"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU4182994.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6369..8765)
                     /locus_tag="VVL53_000796"
     CDS             complement(6369..8765)
                     /locus_tag="VVL53_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU4182995.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRSGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGSTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8831..9145)
                     /locus_tag="VVL53_000797"
     CDS             complement(8831..9145)
                     /locus_tag="VVL53_000797"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU4182996.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VVL53_000798"
     CDS             complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VVL53_000798"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU4182997.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9926..11539)
                     /locus_tag="VVL53_000799"
     CDS             complement(9926..11539)
                     /locus_tag="VVL53_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU4182998.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11730..11984)
                     /locus_tag="VVL53_000800"
     CDS             complement(11730..11984)
                     /locus_tag="VVL53_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU4182999.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(11988..12242)
                     /locus_tag="VVL53_000801"
     CDS             complement(11988..12242)
                     /locus_tag="VVL53_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU4183000.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12480..13577)
                     /locus_tag="VVL53_000802"
     CDS             complement(12480..13577)
                     /locus_tag="VVL53_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU4183001.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13587..14333)
                     /locus_tag="VVL53_000803"
     CDS             complement(13587..14333)
                     /locus_tag="VVL53_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4183002.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14436..14789)
                     /gene="rsfS"
                     /locus_tag="VVL53_000804"
     CDS             complement(14436..14789)
                     /gene="rsfS"
                     /locus_tag="VVL53_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU4183003.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYLVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(14802..15302)
                     /locus_tag="VVL53_000805"
     CDS             complement(14802..15302)
                     /locus_tag="VVL53_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU4183004.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15303..15896)
                     /gene="yqeK"
                     /locus_tag="VVL53_000806"
     CDS             complement(15303..15896)
                     /gene="yqeK"
                     /locus_tag="VVL53_000806"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU4183005.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLVLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(15896..16525)
                     /locus_tag="VVL53_000807"
     CDS             complement(15896..16525)
                     /locus_tag="VVL53_000807"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU4183006.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16577..16888)
                     /gene="yhbY"
                     /locus_tag="VVL53_000808"
     CDS             complement(16577..16888)
                     /gene="yhbY"
                     /locus_tag="VVL53_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU4183007.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17102..18208)
                     /gene="yqeH"
                     /locus_tag="VVL53_000809"
     CDS             complement(17102..18208)
                     /gene="yqeH"
                     /locus_tag="VVL53_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU4183008.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(18211..18801)
                     /locus_tag="VVL53_000810"
     CDS             complement(18211..18801)
                     /locus_tag="VVL53_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU4183009.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(18753..19661)
                     /locus_tag="VVL53_000811"
     CDS             complement(18753..19661)
                     /locus_tag="VVL53_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU4183010.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESETVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19718..20278)
                     /locus_tag="VVL53_000812"
     CDS             complement(19718..20278)
                     /locus_tag="VVL53_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU4183011.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20417..21626
                     /gene="sstT"
                     /locus_tag="VVL53_000813"
                     /pseudo
     CDS             20417..21626
                     /gene="sstT"
                     /locus_tag="VVL53_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21694..22647
                     /locus_tag="VVL53_000814"
     CDS             21694..22647
                     /locus_tag="VVL53_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU4183012.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT         6101 a         5363 c         4458 g         6792 t
ORIGIN      
        1 gaaagcgtta tcaatttatt tatctcattt ttcagaaaat tcttttattt ctgtaaagtc
       61 tacgatactc gatgtgtttt tatataatga tagagtctga gaatcactgt tccgctagcc
      121 attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc cagtgcttta
      181 ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc tggaaccagc
      241 ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat acttaaaatc
      301 tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac attggtcggt
      361 tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc aggtaagagc
      421 atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa acttgctact
      481 gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg cgacagaagt
      541 tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca taccagagtt
      601 tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg gtacaaacgg
      661 catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt agcgagccca
      721 aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa agggaattcg
      781 gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca ctcctgcccc
      841 aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac taaaggtcgc
      901 agccagagtt acctgcaact tagtataggg aaggggtttg gcttgcaagg ccgtcaattg
      961 cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa tctggttcac
     1021 atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc gcgaaatatt
     1081 ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc aatccacaat
     1141 cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt ctgagccact
     1201 tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta attcttttga
     1261 ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat ccggactcaa
     1321 aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat tatccatctg
     1381 cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga ctctagtctt
     1441 attgaggtct accttttcac ctgctctagg actttgttca acaaccatgc cttctgcact
     1501 acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa tcccaacaat
     1561 ttgaatcaaa ttgttcttag taaactccaa gctagaacca atgtaactcg gcatggcaac
     1621 acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca catcataagt
     1681 cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc tggactcttc
     1741 ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg agattacttc
     1801 tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt tagctactgt
     1861 caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta gactttgctt
     1921 caggaccgtt ccagcctcac tctcattcga ctcttcttcc tcaattttaa tcaaattatc
     1981 tggaactttt ttctctttta attccgcaat gacatcagag gatttccgac cgacataatt
     2041 actaatttgg aaagattgct tgcctgatga gacaaccaaa ttgattttcg ttccttcttt
     2101 tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca ccttttcact
     2161 agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg ccttggcctc
     2221 tgcaactgtc tgacctgcca catctggaat ggcaatggtt gcaggagttc tggatagtat
     2281 ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt aacgcatctt
     2341 aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag cctggcttgg
     2401 gtttttgatt gatttgtgtt ctgtttgcgc ttgaacctta ggaatagatg tcaaggtact
     2461 ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa agattaactt
     2521 actttcattt ctacgattgt aggacaagct actagacaag tccacataca tctctgaaac
     2581 cgagcggtag cgattggtca actttttagc agttgccttg ataataacat tttctaaagc
     2641 ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga aatgctggag
     2701 ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcagca tctcatagaa
     2761 aataatcccc atggcataga tatcactctg cacagtcgcc ttcgaaccac gcgcctgctc
     2821 tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac ttgtctctgc
     2881 aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg tcaagaggat
     2941 attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagc gcatagccaa
     3001 gagaatttgt cccatgatac ggactgcttc ttcattagaa agaggataat gttccttgat
     3061 atagcgtttg aggtccagtc cagccacata ctccatagct aggtactgtt gaccgtcttc
     3121 ctcgccaata tctgttatcc gaacgatatg aggatggtct agatctgcca tagctctcgc
     3181 ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc tcagaacctt
     3241 cactgccact tcttccccat ctaagattaa gtctttggct aggtagacat ccgccatacc
     3301 tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc cgatttggat
     3361 cattctgcat cctcctcgtt catagaaaca agggcaaccg taatgttgtc taaacctcct
     3421 gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat atcactggtt
     3481 acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact attgagcaag
     3541 agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc atctttttgc
     3601 ccaatagact gggtgataat atttttttgc ggatgagctt ctgcctcttc tggtgtcaat
     3661 tgaccagcct tgagcaattc attaaccaag gaatgatcgc tcgtcaactg atggtattct
     3721 tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc ctgattatca
     3781 ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc ctgaccaagc
     3841 tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg cacttcattg
     3901 actgtatcga tctgggtatc aacccaagct acacccaggt ctgtgaccgc catttcacta
     3961 gcgatattcc ctgcgcgatg acctcccatc ccatcagcta aaataatcat ggtacgtcca
     4021 gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg accaacatct
     4081 gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg cgaaattgac
     4141 tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag ccgtctttca
     4201 tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga ctctctaaaa
     4261 aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta taagttatta
     4321 taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga atttcctgca
     4381 aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg cgcaaaagac
     4441 cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg tcaaaaaact
     4501 catgcacctt tctggcatcc aatttttgag tttgaacccg atcttcaact cccagacgtt
     4561 gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca gtaacctgac
     4621 ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca caggcatcaa
     4681 gcactcgctc atcaccttgt aaatcaagcg tcggagcaac cagctgactg gactcatctt
     4741 ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc tgctccttaa
     4801 ccagaccagt ggttgctaaa agggaattat tcgcctccaa caaggcttgg atttcctctt
     4861 ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg ctttcaaaga
     4921 tggcttttgc tctctcctct ccgtattctt ccttgagttt ggcaactagc caaactggga
     4981 gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata tctggccagc
     5041 cttcacgcaa gatacggcga agaacagcgt tgaccaattt ttcactgcct tttttacgga
     5101 gtttggccaa ttccactgct tcattaacca cagcatgatc tggaatcttg tccaaatagc
     5161 ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac tggtctctgt
     5221 cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc gttccataga
     5281 ccagctcggt cactaagccc ttgtctgctg ccaaaagttg acttcccttt agatgcttat
     5341 ttaaggcgat atttgaatat gcttggttca caaaaacatc ctctagcact gctagagcta
     5401 aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa tgtacgtcca
     5461 actccgttga ggaaggaagc aatgtccatc ttaggcttac cagctggctg cacttgtttg
     5521 agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat agagagaatc
     5581 tcacctggat ttccctgacc ttctactggt agggcttcat aaatcttaaa gcggtcgccc
     5641 ttaaggaaag tatgggcaac aggccagggg ttcattccac gaatttggtt aaagagttga
     5701 cgattggttt tgttccagtc cagtttttct tcctctggct ttatatttgg agagaaggta
     5761 acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc aggcagagtg
     5821 tccaaaagca aatcacgacc aactagcgcc aatttttcaa acaaggtgcc aacattgtcc
     5881 tcatctgtga tcggaatgct gcgacgagaa atcatatctc ctgcatccat ttccttaacc
     5941 atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata atggataggc
     6001 gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa gtccatgcta
     6061 tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc atctgctcct
     6121 agcttcataa gatcttccat ctctggactt ccagataatt tttcaggttg gtagatagat
     6181 agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt tttacgacca
     6241 acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc tgtcaaaagt
     6301 ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt tgtcatatct
     6361 tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag ctcactattt
     6421 tcccgttctt gagtcaaggc taaaacctgg ttgagggtcg accccagctc atcttctaaa
     6481 cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat cggttttggc
     6541 gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt catgacttca
     6601 taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc aatcgtgaaa
     6661 tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata aaagccttcg
     6721 taatcttgat ccttggcaaa tcgaatagca tagtgctgcg gattgtagga ctgtatcaag
     6781 acttgacctg ccttttcagc ccgacctgat cgacctgcca cctgagtcaa gagctggaag
     6841 gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt tagaactccg
     6901 actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc aagtaaaata
     6961 tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt ctttcgagtc
     7021 gtatccacat ccatcctcag aatgcgagct tggggaaaga gttctgctag ctcatcataa
     7081 gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacaatt aggacagacc
     7141 tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt atccatatgc
     7201 aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc ccgacacatg
     7261 acaaagctag aataaccacg gcgattgagc atgagaacca cctgctcttt tttaaccaga
     7321 cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt ttgtccgata
     7381 tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt ggcacgttgg
     7441 gttagacgta agtgttgata gacgcctttg ccagcacgtg cccggctctc taagctcggc
     7501 gttgcagatc caagtaccag agttgcttga ttatactgag cccgtaaaat agctacctct
     7561 ctggcatggt aacggggatt gctgtcctgc ttataagccg cttcatgctc ttcatcaata
     7621 atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc aacaacaact
     7681 tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcaccatt ggataatcct
     7741 gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt catctgagga
     7801 gtcaaggaaa tctcaggtac cagcaaaata gctgtcttgc ccttatccag ggcaccttgg
     7861 ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag tagaaaggga
     7921 ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg ttctggattt
     7981 aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg ttgaacttcc
     8041 ttttggacta tggtaacagc accttgatcc acaaagaagt tgacttgctc tcgcgagtag
     8101 gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata atctctcagt
     8161 tccaactttt tcttggcacg tgtagaaatc tcaacacctt ctaattgagc atggtcaacc
     8221 tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc cagaccaagc
     8281 aggccttttc tagtcaaacg catcatttca gcttgcttgg caaggtctag tgaagaaaag
     8341 gctagcgaat cttctgaacc aaacaggcgc actcgttctt cctgactcaa gccttccaga
     8401 ggatagagaa tcttgtcata gctagaattc agaaaccctg gaagcatggc cttgaggata
     8461 gagattttgt aggagaagac agatttgcgt aactcctcag ccagccagag ttgttctggc
     8521 gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg ctccatctct
     8581 tctccatctg attgggactt caaaccaaga acaatccctt gaatcaggcg attaccctta
     8641 ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc ctccggaatc
     8701 ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat cttagctagg
     8761 gccatcttct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat aagattgagt
     8821 ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc tctttgattt
     8881 tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc actgcttcac
     8941 gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct tctaaagcgc
     9001 gaagagttga tttttcagac ttgaaacctt gagttgctgg ggcacctgct tccaattcgt
     9061 gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg tcgagcaagg
     9121 tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt tatcgggtag
     9181 ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca cagaagtgtt
     9241 ctgcttcaat cacacatttg acacgttcag cagctagggg tacctgatcg ttgacaatcg
     9301 cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt cgttgggcaa
     9361 tcacttctgc actatctgtt ccacgaccta ccaagcgatc ttgcaattca tccaaatctg
     9421 gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga agagcaccct
     9481 gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg acataggtca
     9541 gaggagttcc atagtagtta ccgacatatt ctgcgtattc caacatctgt ccttgacgaa
     9601 tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc acttctccag
     9661 gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca gaactctcaa
     9721 aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa acgattagta
     9781 agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat ttctctagaa
     9841 taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac aaaaaagcag
     9901 taatttttca actgctcttt cttatttatt tagcataatc tactgcacga agctcgcgaa
     9961 tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc ttacgaactt
    10021 tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc atgatacgaa
    10081 tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg ttagcaattt
    10141 cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga gcacctggac
    10201 gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc tcagcttcaa
    10261 catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg tacttacggg
    10321 ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg gctttcccga
    10381 tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt tcgctcgcca
    10441 tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt ccatatgaag
    10501 tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt ggcgcaccaa
    10561 tttcataggc agcagcctca ccgtattcac gaatcttatt gtcaatctct tgacggtttt
    10621 tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc aacatttcca
    10681 tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc accacttctg
    10741 gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga atgttacgac
    10801 cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact gttgagtttg
    10861 ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag atgtccttgg
    10921 ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga ctggcaatct
    10981 ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct gcctgagaca
    11041 gcgcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct aattgctctt
    11101 cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt tcaagtgttt
    11161 gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc tctgtcaaac
    11221 gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc acttcttcac
    11281 ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga gacttgcttt
    11341 cacgtttggc ttcattaaca agtaaatccg cttcacgctc agcttgtcca cgtaaattag
    11401 ttgcttcttg ttcagcattt aaaagcatca actctgcagc ttcctgagat gatttcatct
    11461 tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa acagcaatcg
    11521 caagcgacat gatttccatg tttttacctc attttattgt tattccgaat gacatacatt
    11581 cttttacatt ctaccataaa aaagtgattt tcacaaacct aaaatagaat atgttttgag
    11641 gaatttggaa cacatttacc aaaataaact tgttgtttag aaatagtagt ttagtagaga
    11701 cttgagaaaa agcctacctt tcaatagact tagtaatgat ctttaaagga caagaaagcc
    11761 acgctatctc catccatcat ataaatcaag cgattttctg catcaatacg ccgtgaccag
    11821 gctccttggt aatcatattt gagtggttct ggtttaccta ttcctgtaaa gggatcacgt
    11881 tgaatatcct tgattagttt attgattctt tttaacgttt tcttatcctg attttgccag
    11941 tagcaataat ctgcccaggc atcttctgta aacttgagca gcatttctta ctcctcaata
    12001 acatggacct gagtacttcc agcacgaact tgagccattc ctcgcaaaac cttatcagaa
    12061 agttccttat tttgagcaat tctcagggtt tcttggatac tatcccactc actctttgaa
    12121 aggactacaa tgtcctcatc tggattttta ttgaccaccg tcaaaggctc aaattcatca
    12181 tttaccttct tcatgtagtc ctttaaatga tttcggaatg ttgagtaaag gactgcttcc
    12241 ataaccatac ctcgttttag ctcttttcca ctattataca cgaaaagaaa gaaattgtca
    12301 ggaacttgta caagattttc ttttctatct atttatactc aatgaaaatc aaagagcaaa
    12361 ctaggaaact agccgcaggc tgtacttgag tacggcaagg cgacgttgac gcgatttgaa
    12421 tttgattttc gaagagtatt attcgtaaaa aatctcaaaa agcctacctt tcggtagact
    12481 tagtttgttt ctattctaat cggcactctt ccaaaatttt gctctgctat acttggcttc
    12541 cctagttggt aaatctggtc tgctttttgg gtcatagcat cccaaggttc tttgccaatt
    12601 cggctgacta gactgacctg acctttcaaa gacttgagat gttgcctacc tttttcagta
    12661 aatccaagga catgaatggc ttctggcaag tcactttctc tagcctgcat caaaatatag
    12721 gtcaataggc gtctgacacg cgccttggtg taacgtttgg tagtcactaa ctcgaccaat
    12781 tcttccacag actgggctgt tttaatagct tctttaatgc gcacagccat ttcttgattg
    12841 acctgataga tggtggttag gtcgggattt gacaagattt gatagcggag caagggaaaa
    12901 tagtcttccc agatcacctt actggcctgc tcaaataggg caacagaagg cataaaacgt
    12961 tctaagaaat cttggtcctt ctgatgttga cggagggctg tcgccgaggc aaagtccaca
    13021 tctttattca cagaatggta acctgccccc tgacgctgaa tcggatgcag cttgatattc
    13081 cgtcctgcaa ccgccttggc ataggccaga gcaagaacat gattgggggt attacctgaa
    13141 aaatcaagac cagcaaattc cttccacata gcttgggttt tctggggata ggaaagggaa
    13201 tcaggcagat tttccacaaa tttctccatc tcagcacctt tctctgtgta taagtcagca
    13261 attttctgat aatctcgaac ttcctctgtc ccaaaaacta gactatcaat gcccaagcga
    13321 tccaagatat ccatagctcc ttgaccaaag aaatcagctg cctgaacact gactaaaaag
    13381 ggcaattcca ctaccaagtc tgcgccattt tccagcgcca tctgggtcct tgtccactta
    13441 tccacaatag caggctctcc acgctgcatg aaattccctg acatggcaac aattttcagt
    13501 ccctctgcct gatccagcag gtatttatgg ccattatgaa aaggattgaa ctccgcgata
    13561 atacctgtta tggtcatgct aatctcctac ttctgcgcca caaaaaacca acgggtgcta
    13621 gtttctgttg gctccttatc ctcaaagtct gcatagagtt tgaaggactt aaatccagcc
    13681 tgttccagca agatatcata ggtcaagatc tcataggtcc tctcctcatg tacttcatcg
    13741 tggcgactaa aggaaccgtc agcctccttg ataaagaaag tcagctcatg cacgatggag
    13801 tgaggaactt cgtcctcata tgtatcccaa agcatggcaa aatcttccgc attttcatgg
    13861 taggaatagc cagggaatac ttcatctgtc tggtaggtcg agtgcacgtc aaagatgaaa
    13921 actccctctt cattcagcgc attgtacacg tccttaaaga cgtcccctac ttccacctca
    13981 tcctgcatat agcagataga gtccgaataa cacgtgacaa aatcgtattt ccctgccttg
    14041 gacaaattca gcatattacc ttctataaaa gcaatctttt gcttggctga agttgctctc
    14101 ttctccgcaa tcttcaacat atccgcactc aagtcaagtc cagtcacatc aaaaccagcc
    14161 tgagagaagc gcactgattg aattcctgtc ccacaagcca attccaagag tttctttctc
    14221 tccttggtct taggcaaatg acgcagagaa aaattcgtcc atttgtcgta taaactatcg
    14281 tccatgaccg catcatagac cgccgcaaag gtttcataag tcgccataat catgatacta
    14341 agagctccat ggtaaacacc actagccctt tacctttcta tcaatttttc taaaataagc
    14401 aaagaaaccc agttgactac aactgagttc atcttctaag caagagcttc tgaaatatct
    14461 actgaattcg cctcatgcca tagcttctct agattataat gggcacgcat ttcttctgaa
    14521 aagatatgca cgacaacagc accgaggtcc agtaagaccc agcctccagc tgcatcgcct
    14581 tcgacatggc tacctttaaa gcctgcttga gctacttttt cacggatatt agctgcgata
    14641 gcgtccaact gacggctatt cattgagcta gtgatgacca agtagtccgt cacactagtc
    14701 aaatcttgta catcaagtgc gaggatatcc tccgcacgtt tctcatcagc cgctttcacg
    14761 actagttcta gtaattcttt ttcgttcatt taatcctctt tttagaaaat atgtttgtaa
    14821 tctagaacat ctgcaaagcg ctcctcccaa atgtcctcat aaaattcatt aatcgtttct
    14881 gctggaatgt catccccatc aaaggttgtg acagcacctt ctggaacaat aagctgatag
    14941 ccatattcaa aggcaacctt gacagaggta tccacacaat attctgtctg cataccacat
    15001 aaaactaatt tttcaatccc ctgcttatcc aagtattctt ttaagccagt ttctttgaaa
    15061 atactgttat acttcttctg aaagaccttt tcagcaggtt ttcgatttaa aagcgcagat
    15121 aactgccaat cttctgatgt ttgagcttca gagttctcaa tatgttgaac atagataatt
    15181 tcaatattct tgcttctagc ctggttttgt aaataagaaa ttttttccaa tagacttttt
    15241 gtctggaacc cagtttctac taaaacattc tgaacatcaa tgattataaa agccgtcttc
    15301 atttagtcct ctttcaaata gtgcacaaag gcgttatagg tttcaagggt ttggggatag
    15361 atggggaatc cctgatgagc tagatactcc acggtacgag ctgtttcgta ggccaccgcc
    15421 ttattgagcg atagacttgc aatctcacgc gccacatcta ctccaggaaa ggctcgattg
    15481 tgctcgatat agtctgcgac gtagatgacc ttatctaggt ctgtcatttg accagctccg
    15541 actgtatgaa tttcaatggc tcgcagaatt tcagaatcat gcaaatccaa atcttcctga
    15601 attttgtaaa ttccgaccat accatgccag acattattgc cccagttttt gaggtcaggg
    15661 tctagctggt aacggtcaat caagactaga aattcctgat ctgacaactt tttagcatag
    15721 tcatgaagaa gacctgctag acttgctttc tcaacatcta caccaaagcg ctgagctaat
    15781 tccatagcgg cacgctccac acctagacaa tgggttagac gtttctcagg tagaagctct
    15841 gccatttttt ccaacaaggc ctcacgggag cagttgatat agtcttgata ggacatcagt
    15901 agagcccttc cttctcgatg tagtctagca ctggctgagg taggagaaag ttgggtttcc
    15961 gaccttgggc aaggaagtcc cgcaccatgc tggacgagat atccatgagc ggtacgtcca
    16021 cccagataac tggataggaa gtccctacct tgtagcgtgg acgctgaacc cccacaaact
    16081 gaaccatgtc aaccagttca tcaattcggt accacttagg cagatagtca accatgtcgg
    16141 caccgatgat aaagtaataa tccgtatctg gattcttctc tgtcaaaatc ttcatggtgt
    16201 cgtaggtgta ggaaataccc ttgcgctcca actcaatggt ttcaatgact aggccgtcaa
    16261 ttccctcaat tgccaactca agcatcttga gacgatggtg ttcagggatg gtttcctttt
    16321 tatcaacgtg aggaggttgg tattcaggca tgagaagaac ttgatccagt cccaactgtt
    16381 gccgtacttg atccgcaaca atgagatggg cattgtgaac agggttaaaa ttccccccta
    16441 aaatcccaac ttgtttgcgt tttttctcct tgatttctgg ctccaactct accttggtaa
    16501 agggagtcaa tagttcgatt gtcataggct agtccccttt atttctctat aaaaacagtt
    16561 atttggagtt tcggtcttag atttctttga ctttcttaga aatcttgcga ttttctttct
    16621 tgctagattg tttaaacaaa atcaagatgc gtcctatttt ttggactgta tccacaccga
    16681 tttcttcttc caaaatttca gctacttcgt ggatgttttc atctgtgttt tgtaagagag
    16741 taaccttgat taattcacgc gcatcaagag cttgacggac gctggttttg atttggtcgt
    16801 tgagtccatt tttcccgatt tggatgatag gtttgagggt gtgtgcctgg ctgttgagga
    16861 aggcacgttg ttttgatgtt aatgacataa ttttcttcct tgttttttga tagttatttt
    16921 aggtggtgag ggacggtcgg aactaatttt ccaaagattt catctttgaa aaatggattt
    16981 cctgacctca caattgagtc ttggtctcaa ttgtgcccct tgccaatcta cagattggca
    17041 atcacaccac agcaataact atcgctatat gagctcactt tgttcgctca gcgattttgt
    17101 tttaaataat tgcttttcgt gtgacgacgg cgacgccttc tggtgcccag acggcgactt
    17161 ttgctatgcc tgttacacga atccatccta ggcctgagat aactaggtct gtcttatctt
    17221 taatggtaaa gacatgctgg actagccttg ggaaatcttc tttttccttg ctatttggtg
    17281 gtgtcagaag agttcccagg tgcttatcgt agaaagcact agctccttca agcttgctac
    17341 gatggagttt gagttcatta tcaaagaaag cagtaaatcc ttgcttttct cctgctataa
    17401 agtcaaagcg tcccaaacca cctaaaaata gggtttgctc aggattaagc tgataggtct
    17461 taggcttgat ttccttttta ggactgacat acttgaggtt tttggccgtc aagtagtgag
    17521 ccatctggtg gcggtggata attcccggcg tatcgtaaat ataagatccg tcgtcaagcg
    17581 gaatctctat tttgtccaag gttgtccctg ggaagcgtga agtagtgatg acattctgat
    17641 cacccgtgat ttcttggata atagcattga ttagagttga ttttccaacg ttggtcacac
    17701 cgaccacata gacatcgcgg cccttacggt agtgttcaat cttgtcaatg acttccttaa
    17761 tggcatgttt attttgtgct gaagttagga ccacatcgac tggacgaaga ccttcttcat
    17821 gggcacgttt catgagccac tggctaatct taccagactt aactgactta ggaaggatat
    17881 cttttttatt tcctaccaag aggacatcat tgcccgagac gaaacgtggt aaacctggga
    17941 tgacagatcc attaaaatca aagatatcaa taacattgac cactaaagca tcactgtctc
    18001 ccacctcgtg caagagcttg aggaaatcat cgttcgtcaa ctggacatct gtgatttcat
    18061 tgtagtggcg gagacggaaa cagcgttggc aatagacttc gccagtctcc aaaccttttt
    18121 caagtgccga ctggggggta aaaccaagac cagctttatc tgtcgtctga atggttgctc
    18181 cacaaccaat acagagaatt tcttccatag ttaaattcct tttttatatg taatcggtcc
    18241 gtacttttca gtgatttttc tcataacacg acgctcacga gttcggttaa tctgcgtttt
    18301 gattgagtca tgttggacca agggtttgac taaaattgac cgaatccctg cacggtgggc
    18361 tgctcgtata tctgtcatga gttggtcacc aaccatgacc acttcctttt tgtcatagtg
    18421 gaattccttc atagcacggt caataccaaa tgtgaagggc ttcaaggccc agtaaacgta
    18481 atcaatccca aatttctcaa ctgctcgttg aacgcgtttt ttggtgttat ttgacactac
    18541 gataatgcca atacccgcgt cccgaaggtc atgtagccat tgcttcatct ctggcgttcc
    18601 atcagggttg ttccaagcaa tgagggtatt atccaaatcg accaaaacag cctttattcc
    18661 ctgcgcctgc aggcttggga ctgtcagatc atagactgct tccacagcaa aatctggtat
    18721 ataattttca atcgccattc tggctccttt tcttaacttt ttttcgcaat tttccttagt
    18781 aacaaggata aaacaatcca caaacctgca ctagccaaac tgatatagag ccaagcatgg
    18841 ggctcatctg ttaaaggtaa gggaacattc attccgaaaa agcctgtcac gactgccaaa
    18901 acagctagca agactgaaat gatagtcaag gttgtcaaat tgtcattcag attattgttt
    18961 agaatattgt tgtaagaggc tgaaagctgc tgtaaaatct gagagattag gtctgtcatg
    19021 gataccagct gatgagcctc aatcatggca tcatcaaact gttctctctc aatctcatca
    19081 aaactacgat acaaggcatg accttgaata tgctctaaca aaatccgatt ttgtttggca
    19141 gctgccgtca gataaaccat accagtctcc aaatcagaca ggacaaagag gtttttcttg
    19201 gtagttcgct ggcgcaagag gtcattgacc tcatccctac tcttgtccat ctgctcaatg
    19261 acaggatagt aggcattgct gatgatttcc agactggcaa agagaaactt ataaatcgaa
    19321 agcgtgtcat ggttctccag ataacgagtc atctgttcaa tgacataggc gttcttggta
    19381 ttactaatgg taatcaggcg acgatgctcg acaataaagg tcatgggaaa agtctcatag
    19441 taggccttgt ccttttttac gtctaagaca ttatagataa aggtaaccgt ctcactttca
    19501 cggtggtagt ccatgtgggc gcgctcgttt ctatccagtg cgtattcaat ggtttccttg
    19561 tccaaaccgt aaatttcaga aaggtcttct agtttattca actttcctag gtctaggtct
    19621 atccaggtac aaccattgcc caactgcttt tctaaaacca tcttttctcc ttattaaact
    19681 ccctctattg taccacaaat tacttaaaat aacaggtcta gtctgtgcgt gagaaattgc
    19741 taacaactgt gatatttcgg ttagggacaa agtgaagggc ttgctcctcg ctacgaagct
    19801 gtgtcgtacg aattcccaca ctgacaacct tacccgatac agtaatcggt ccatttgtca
    19861 gaacgacctc atctcccaca tccagttgac gttcaaagag gatgaaaaag ccattgatga
    19921 catcagacag aaagccttgg gctcccatac caatcgctac cccagcaata ccagctccag
    19981 ccagcaaact agaaactggc aaacctaaaa tcgacaaaat gcagtagagt aaaaagaaat
    20041 atagcgtata attaaacaca ttttctagta aacgtgagat ggttttttgg cgtccaacat
    20101 catgacgaga catttttaga gaaggtttga caattctctg caccatggta tgaagcattt
    20161 ttttagcaat ataaaataca attaaaagca gtaaaagaga aatgaccttg gttagaatat
    20221 tctcgataat ggttgtcaca tctagctttt caatataagc ctgaataaat ttttgcatat
    20281 aaaactcctt tatttcatta taccatattt tcatcagtct cattctccat aaatattcaa
    20341 aaaatagaca gaaaattctt gattttgtct atcatttata ctataatcat aatcactaca
    20401 caaaaaggag attattatga aaagaatcat tcatgcttgg aataaggcaa gccttgtcaa
    20461 gcgtatcttg attggtatgc tttgtgggga atcctaggac tgacccttcc taatctctca
    20521 gggattgggc tactcgagga tttattcgtt ggaggtctaa aagctgttgc tcctatccta
    20581 gtctttgctc tcgttgccaa tgccctttcc caacatcaaa agggacaaga tagcaatatg
    20641 aaaactgttg ttttcttgta tatcctatga tagggacttt cgccgctgct cttgtagctg
    20701 tactagcaaa tttcatcgtc cctattgaaa ttaccccaaa tagtgccaat actgaaattg
    20761 caccaccaga tgggattggg caggttctca gcaacctctt gctcaaactg gttgacaacc
    20821 cagtcaacgc cctgcttact gctaactata ttagaatctt atcttgggca gtcatttttg
    20881 gaatcgctat gagagaagcc agtaaaaata gtcaagaatt gctaaaaact atcgctgacg
    20941 tgacttctaa aattgtcgaa tggatcatca atctggctcc atttggaatc cttggtcttg
    21001 tttttaaaac catttctgac aagggagtcg gaagccttgc caactacggt attttattgg
    21061 ttctattagt aacgactatg ctttttgttg cccctgtggt caaccctttg attgccttct
    21121 tctttatgag acgcaatcct taccctctag tttggaactg cctccgtgtc agcggtgtga
    21181 cagccttttt cactcgtagt tctgcgacta acattcctgt caacatgaaa ctctgccatg
    21241 accttggact caacccagat acctattctg tttctatccc actcggttct actatcaata
    21301 tggctggagt agcgattacc attaaccttt tgacccttgc tgcagttaac actcttggaa
    21361 ttcctgttga ctttgccaca gcctttgtcc tcagtgtggt agcagctatc tcatcctgtg
    21421 atgcttcagg tattgccgga ggttccctcc ttcttatccc agttgcttgt agccttttcg
    21481 gtatttctaa cgatattgcc atacaaattg ttggggttgg ttttgtgatt ggtgtcatcc
    21541 aagactcatg tgaaacagcc cttaactctt ctacagatgt cctctttacc gccgttgccg
    21601 aatacgcagc aacccgtaaa aaataactca tcaaggcaag cctgctcatg tcttgtcttt
    21661 tacgctttta ttctaactta ttaggaaatt cttatgtcta ttagccaacg tacgaccaag
    21721 ctcatcttag ctacctgtct tgcctgcctg cttgcttatt ttctcaatct ttcgtcagca
    21781 gtttcggctg gaattatcgc tctcttgagc ctatctgata cgcgtagaag tactttaaaa
    21841 ctggctcgca atcgtctttt ttctatgctt ctagctctgg ctatcggtgt tctagctttt
    21901 cacttgagcg gatttcatat ctggagtctc ggcctctatc tggccttcta cgttccttta
    21961 gcctacaaga tgggctggga aattggcatc acaccaagca ctgttttggt tagccatctc
    22021 ttggttcaag agtcaacctc tccagacctt ctagtcaatg aattccttct ctttgctatt
    22081 ggtacaggat ttgccttgct tgttaatctc tatatgcctt cacgagaaga ggaaatccag
    22141 cactaccaca cgctggtgga agaaaagtta aaagatatcc tccagcgctt caaatactat
    22201 ttatccagag gagacggacg caaccgagca cagctggtag cagaattaga cacgcttttg
    22261 aaagaagccc tcagactggt ctatttggat cactctgacc acctctttca ccagacagac
    22321 taccatatcc actactttga gatgagacag cgacaaagtc gtatcctgag aaacatggcc
    22381 caacagatta acacttgtca ccttgccgcc agtgaaagcc tgatcttagc gcaactcttt
    22441 tcaaaaattg caggtcaact gagccagacc aatcctgctt ctgatttgct agatgaaatt
    22501 gaacgttatc tggaagtctt ccggaaccgc agtctgccca agacaagaga agaatttgaa
    22561 acccgcgcca cccttcttca actcctacgt gaagccaaaa ccttcatcca agtaaaagtt
    22621 gatttttacc aaaaatatag acagtaatac tcttcaaacc acgtcagcgt cgccttgctg
    22681 tatatatgtt actgacttcg tcagttctat ccac
//
LOCUS       DAVHEW010000022        22417 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVHEW010000022 DAVHEW010000000
VERSION     DAVHEW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.022"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            28..795
                     /locus_tag="VVL53_000815"
     CDS             28..795
                     /locus_tag="VVL53_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU4183013.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            880..1155
                     /locus_tag="VVL53_000816"
     CDS             880..1155
                     /locus_tag="VVL53_000816"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183014.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFLQQYQQI"
     gene            1245..1559
                     /locus_tag="VVL53_000817"
     CDS             1245..1559
                     /locus_tag="VVL53_000817"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU4183015.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(1660..4212)
                     /locus_tag="VVL53_000818"
     CDS             complement(1660..4212)
                     /locus_tag="VVL53_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU4183016.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQSIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4274..5896)
                     /locus_tag="VVL53_000819"
     CDS             complement(4274..5896)
                     /locus_tag="VVL53_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4183017.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            6102..7127
                     /gene="trpS"
                     /locus_tag="VVL53_000820"
     CDS             6102..7127
                     /gene="trpS"
                     /locus_tag="VVL53_000820"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU4183018.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            7279..8757
                     /gene="guaB"
                     /locus_tag="VVL53_000821"
     CDS             7279..8757
                     /gene="guaB"
                     /locus_tag="VVL53_000821"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU4183019.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(8808..9905)
                     /gene="recF"
                     /locus_tag="VVL53_000822"
     CDS             complement(8808..9905)
                     /gene="recF"
                     /locus_tag="VVL53_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU4183020.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(9908..10276)
                     /gene="yaaA"
                     /locus_tag="VVL53_000823"
     CDS             complement(9908..10276)
                     /gene="yaaA"
                     /locus_tag="VVL53_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU4183021.1"
                     /translation="MEYKLFEEFITLQALFKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            10435..11685
                     /locus_tag="VVL53_000824"
     CDS             10435..11685
                     /locus_tag="VVL53_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4183022.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            11682..12965
                     /locus_tag="VVL53_000825"
     CDS             11682..12965
                     /locus_tag="VVL53_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4183023.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGEIIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13007..13828
                     /locus_tag="VVL53_000826"
     CDS             13007..13828
                     /locus_tag="VVL53_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4183024.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            13839..14384
                     /gene="pgsA"
                     /locus_tag="VVL53_000827"
     CDS             13839..14384
                     /gene="pgsA"
                     /locus_tag="VVL53_000827"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU4183025.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            14381..15208
                     /locus_tag="VVL53_000828"
     CDS             14381..15208
                     /locus_tag="VVL53_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU4183026.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            15193..16032
                     /locus_tag="VVL53_000829"
     CDS             15193..16032
                     /locus_tag="VVL53_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU4183027.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16025..16819
                     /locus_tag="VVL53_000830"
     CDS             16025..16819
                     /locus_tag="VVL53_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU4183028.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SISLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            16878..17696
                     /gene="mreC"
                     /locus_tag="VVL53_000831"
     CDS             16878..17696
                     /gene="mreC"
                     /locus_tag="VVL53_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU4183029.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            17696..18190
                     /gene="mreD"
                     /locus_tag="VVL53_000832"
     CDS             17696..18190
                     /gene="mreD"
                     /locus_tag="VVL53_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU4183030.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLIYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            18284..19462
                     /locus_tag="VVL53_000833"
     CDS             18284..19462
                     /locus_tag="VVL53_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU4183031.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            19686..20465
                     /gene="rpsB"
                     /locus_tag="VVL53_000834"
     CDS             19686..20465
                     /gene="rpsB"
                     /locus_tag="VVL53_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU4183032.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            20544..21584
                     /locus_tag="VVL53_000835"
     CDS             20544..21584
                     /locus_tag="VVL53_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU4183033.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            21830..22153
                     /gene="tnpB"
                     /locus_tag="VVL53_000836"
     CDS             21830..22153
                     /gene="tnpB"
                     /locus_tag="VVL53_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU4183034.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
     gene            complement(22294..>22417)
                     /locus_tag="VVL53_000837"
     CDS             complement(22294..>22417)
                     /locus_tag="VVL53_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU4183035.1"
                     /translation="KRGFKNKAFRTLEDVIQGLEKEVIKSIVNRRRTRMLFENR"
BASE COUNT         7145 a         3608 c         4589 g         7075 t
ORIGIN      
        1 taaaaaaaac caacctttat tattgtcatg atcgggattt ctcttattcc agatctgtac
       61 aatatcatat ttttgtcatc aatgtgggat ccatatgggc aattgtctga cttacctgtg
      121 gcagttgtaa ataatgataa agaggcttcc tataatggta atactatggc aataggaaaa
      181 gacatggtgt ccaatttaaa agaaaataaa accttggatt ttcattttgt agatgaagag
      241 gaaggaaaga agggattgga agatggcgat tactatatgg tagtgacttt accaagtgat
      301 ttatctgaaa aaacaactac attatccaat attcaatcga cagcagctta tcaatcattg
      361 acaagtgagc aacaaactga gataagtgat tctgtatctc aaaattcaac tgatagtatt
      421 caatcggctc agtcaattgt agctttagta caagatttac agggaagttt agaaaactta
      481 caaaatcaat cttctaatct ttcgacttta aaaaatcaat ctaatcaagt atcacctatt
      541 acttctactt ctttgatagg attgtcaagt ggattaacag agatacaagg agatgttact
      601 agcaaattag ttcctgccag tcagtcgatt gcatcaggtg taaacgcata tactacaggt
      661 gttgataaag tttctcaggg cgcaagtcaa ctaagtgaaa aaaatgccac cttgacaggt
      721 agtttggata aactagtttc aggctcaaac accttgacac aaaaatcttc tagattgaca
      781 gcaggagttg gttaattaca atcaggatct gggcaattag cagacaaatc cagtcagtta
      841 ctttcaggtg cttctccatt agagaataga gctaataaat tggcagatgg atctgggaaa
      901 ctagcagaag gtggaacaaa gttaacttct ggattggaag atttacagac aggacttgct
      961 tctttaggac aaggactagg taatgctagt gatcaactca aatcagtatc aacagaatct
     1021 aaaaatgcag agattttgtc aaatccactc aatctttcaa aaacagacaa tgatcaagtt
     1081 cctgtaaatg gaatcgcaat agctccttat atgatatcag ttgctctttt tttgcagcaa
     1141 tatcaacaaa tatgatattt gcgaaattgc cttcaggacg tcatccagag agccgttggg
     1201 cttggttgaa atcttgagct gaaataaatg gtattatagc tgttttggca ggaattttgg
     1261 tatatggagg agttcagctt attggtttaa ctgctaatca tgagatgaga atatttattc
     1321 tcatcatcct aacaagttta gtattcatgt ctatggtgac cactttagca acgtggaata
     1381 gccgtatagg agcttttttc tcacttattt tgcttttact acagttagca tcaagtgcag
     1441 gtacttatcc acttgctttg acaaatgatt tctttagatc tattaatccc tggttaccaa
     1501 tgagctattc agtttcggga ttacgacaaa caatctctat caacaagtca ttttcctagc
     1561 tgtcatacta gttctattta ctagtttagg tatgctagcc tatcaacata agaaaatgga
     1621 agaagattaa aaaaatcgac cgattaactg gtcgattttt tatgccttag atgactttcg
     1681 tctgtgatta tagattccaa atagtaagag agaagtaaag gaacagattg ctccagtaat
     1741 aaaaccattg ggaatgaagg aaagtgtaat agttcctttc cccttgggaa tgtcaacttt
     1801 cataaatcca gtttgagctt gtttaatttc tattttctta ccatcttggt aggcagacca
     1861 acctttgtca taaggaatgg tgaagaaaat agatgtatct tgttggacat catatgtagc
     1921 aaaaaccttg tttttagaag ttgatactgt gacaggttgt tctttaattt tttgaattgc
     1981 ctcggtgaaa gttttggtat ctaaacgata gaaggtagga gattcaaatg atacttgtga
     2041 atttccaggg aaactaacat tgatattgaa agtttttttc tctttagtat atcctagatt
     2101 aaagaaggag aagacattat cagttgtaaa agtctttttt tcaccattta caaggatgtc
     2161 aaccttcttt tgtttatcgt tagaaaagtg aaggtttatg aaagagagat aaacttggct
     2221 gttttctgga acttcaattt gatactggat tgctgcatct tcatttgaag aacttgtgac
     2281 actaatcaaa tcattagtat tttctatttt ttctgttttt tcataaggta ttggagaaaa
     2341 ataatcaaaa ttgacgttag caagttgatt taaaaatgag gcctgattat ccaaggtatg
     2401 ttcattgaac ttgacatcat tgtaaacaga ttgactcgca actgcaatcg gaagagagta
     2461 ttgattttca tatagggtaa gattatcttt ttgatagata tctttaaagc catacttatc
     2521 aataggactg tctgagatat tgtactggat accaaataaa ctatcagcca aaatactatt
     2581 atttgcatat cggagattga gattagtccc agaggattta aaaccaagtt tatctaaagt
     2641 agagcttgat gaacgatttc gaacagatga aaattgagag attccattgt agttgaattt
     2701 catactgtca tttcctgtct gagtttgtag tttttcagta cgagtaaatt gatttccaat
     2761 atatgttgag aaagattcca tagctgggat atctcgacta taagcacttc gagaagcaaa
     2821 tccccattcc ttagcaattc cgtccatttg agatgaagca tttaaactca tttcaaccag
     2881 tataaataaa gagattagaa tggcaaatag attcacagat ataaactttt tgataactgc
     2941 aaggagtaaa agagaataga caaccaaaaa ttcaagagta agcagaatat tcaaatctgt
     3001 taaaaaagaa taatgcgatt ttagatagat ggtagctaaa aatcctgcta ctacaagaaa
     3061 aagcgaaact aaaaaattcc agactttaag ttctttcaga cgctttaaga cttctgctgc
     3121 tgtgtaaatt aacaaggtag agaaaatcca agcatagcga tgtaaaaaca tgtttggagt
     3181 atgcatgcct tgccaaaata agtcaagagc ttctatgtaa aagcttgcaa ttagaaatgc
     3241 aaagaatatt acatatatga gtttcacgtg aaacttaata gatttcagcg taaaaaataa
     3301 aatggtcaaa ataaagggaa atagtccaac aaaaatcatt gggatggccc catactttgt
     3361 tgtgtcaaag gaaccaatga attgcttagc aaagagatca agataccagc tactttcagt
     3421 ttgaaacttt gtaacttcag tcaatttttc cccatgtgtc tgtaaatcaa atagagtggg
     3481 aagagtcata atcaaactag ccataccagc taaaaaggag ataactatga aatcaagaac
     3541 agatgatttt cgagtcttaa agtcccacga aatttgacag agataccaga aaataagaaa
     3601 caatactgtc atatatccaa aataataatt ttgaataaat aagattgaca gacttgtaaa
     3661 gtacaatagg agtttctttt cagttatcag tagatgtaaa ccagttataa ttaaaggaat
     3721 caagataaaa acatctagcc aggtttttat ctctaattga ctgacagtga aactcatcag
     3781 agcataggaa gtagataagg ctagttttaa aatctgaggg atagattgaa acaatttatt
     3841 caaactaaaa aaggttgaca gaccaatcaa tccaaatttt aagagagttg tcagatagat
     3901 agcatctggc atattcgtta gatcaaaaaa gtaaaccaga ggcgcgagaa aactacccaa
     3961 gtaataacta gatagggcat agaagtttag ccctagacca cttgtaaagg tgtaaaacag
     4021 attactattt ccatgtagga tatttcgtaa ggctacatca aaaataacgt attgatgaaa
     4081 gccatctcct aatagaggag agttgtcgct attccagtag atactttgag atagatatac
     4141 tccagacata atcactacag gaatgatgaa agaaataaaa taggttcgat atgtttttaa
     4201 aaatgatttc atgttacctc gtagaatgat agaaaactca gttggttaac ccaactgagt
     4261 tttgaagttt tatttagtct ttccaaagtt ctttaacttt tgcttgtact tctgcatttt
     4321 ctaggaattc atcgtaggtt tcatcgatac ggtcaatgac gccattttta gataagacaa
     4381 tgatatggtt agccaaagtt tgaataaatt cgtggtcatg gctggcaaag atgattgatt
     4441 ctttaaagtt tttcaatcca tcattcaagc ttgagataga ttccaagtcc aagtgatttg
     4501 ttggatcatc aagtacaagg acatttgatt ttaagagcat gagttttgaa agcatgacac
     4561 gaactttttc tccccctgac aagacattta caggtttgtt aacttcatct ccagagaaga
     4621 gcatacggcc gaggaagcca cgtaggaaag tattgtcatc ttcttcttta cttgcgaatt
     4681 gacgcaacca gtcaagaatt gattctcctc ctgcaaaatc agctgagtta tcttttggta
     4741 ggtaagattg actagttgta actccccact tgacagttcc ttcatagtca atatctccca
     4801 tgattgcacg aattaatgca gtcgtttgaa tatcattttg tccaataagt gctgtcttat
     4861 catctggacg caagatgaaa ctaatattat ccaagatagt ttcaccatca atctttacag
     4921 ttaaattttc tactgtcaag agatcattac caatctcacg ttccgcttta aagttgataa
     4981 atggatattt acgactagat ggcacaatct cttctagctc aatcttatca agcattttct
     5041 tacgtgatgt tgcctgcctt gacttagaag cattggcaga gaaacgagca acaaattctt
     5101 gcaattgttt aattttttct tctgctttag cattacggtc tgctagcaat ttagcagcaa
     5161 gctcagaaga ttccttccag aagtcgtagt ttccgacata gagtttgatt tttccaaagt
     5221 caaggtcggc catgtgagta caaactttgt ttaagaagtg acggtcgtgg gatactacga
     5281 taactgtgtt atcaaagtca atcaagaagt cttctaacca agtaatcgat tggatatcca
     5341 aaccgttagt aggctcgtcc aagagaagaa catctggttt accaaaaagt gctttggcga
     5401 ggagaacctt tactttttca ccgttggcca attcgctcat gttttggtag tgtaattctt
     5461 ctggaatgtt taggttttga agtagttgag aggcttcact ctctgcttcc caacctccaa
     5521 gttcggcaaa ctctccttcg agttcggcag cacgaacccc gtcctcgtct gagaaatctt
     5581 ccttcatgta gatagcatct ttctctttca tgatgctata aagtttttca tttcccatga
     5641 taacgacatc aatggcacgt tcatcttcgt agtcaaagtg attttgacga agaacagaga
     5701 gacgttcatc tggaccaaga gagatgtgac cagtagtagg ttcgatatct ccagctaaaa
     5761 tttttaaaaa ggttgatttt ccggcaccat tagcaccgat taatccgtaa gtatttcctt
     5821 ctgtaaattt gatattgaca tcatcaaaaa gtttgcgatc actaaaacgt agtgaaacat
     5881 cagatactgt aagcaatgtt tttctcctat atgtgtaata tatttattct actagaaaat
     5941 acagaaatat tcaaattttt atttgtcaat tttgtgtaaa ttatatttac agtatccttt
     6001 acacaaatct gtaaaaagca aggctgattt attttgataa attacggtta tttcattaaa
     6061 aaaatgctat aattgaaagg actatatcga aggagaacaa aatgactaaa cccattattt
     6121 taacaggaga ccgtccaaca ggaaaattgc atattggaca ttatgttgga agtctcaaaa
     6181 atcgagtatt attacaggaa gaggataagt atgatatgtt tgtgttcttg gctgaccaac
     6241 aagccttgac agatcatgcc aaagatcctc aaaccattgt agagtctatc ggaaatgtgg
     6301 ctttggatta tcttgcagtt ggattggatc caaataagtc aactattttt attcaaagcc
     6361 agattccaga gttggctgag ttgtctatgt attatatgaa tctagtttcg ttagcacgtt
     6421 tggagcgaaa tccaacagtc aagacagaga tttctcagaa aggatttgga gaaagcattc
     6481 cgacaggatt cttggtctat ccaatcgctc aagcagctga tatcacagct ttcaaggcta
     6541 attatgttcc tgttgggaca gatcagaaac caatgattga gcaaactcgt gaaattgttc
     6601 gttcttttaa caatgcatat aactgtgatg tcttggtaga gccggaaggt atttatccag
     6661 aaaatgagag agcagggcgt ttgcctggtt tagatggaaa tgctaaaatg tctaaatcac
     6721 taaataatgg tatttattta gctgatgatg cggatacttt gcgtaaaaaa gtaatgagta
     6781 tgtatacaga tccagatcat atccgcgttg aggatccagg taagattgag ggaaatatgg
     6841 ttttccatta tctagatgtt tttggtcgtc cagaagatgc tcaagaaatt gctgatatga
     6901 aagaacgtta tcaacgaggt ggtcttggtg atgtgaagac caagcgttat ctacttgaaa
     6961 tattagaacg tgaactgggt cctattcgtg agcgccgtat tgaatttgct aaggatatgg
     7021 gagaagttta taatatgatt caaaaaggta gtgaaagagc gcgtgaagtt gcgggtcaaa
     7081 ccctatctga ggtaaaaggg gcaatgggac ttcattactt taactaagtt tattttacaa
     7141 gaaactatct tttctatctc gaagaaaaga tagtttttta tttacccctg taacatttga
     7201 caaattttta tagaatggta tgatagatac aatataaaaa gagtcaagct caaaaagaaa
     7261 gaaaagagga aacttcgaat gtctaattgg gacactaaat ttttgaaaaa aggttttacc
     7321 tttgatgatg tattgcttat tccagctgaa agtcatgtgt tgcctaacga tgcagattta
     7381 acaactaaat tggcagataa tttgacttta aatatcccaa ttattaccgc tgccatggac
     7441 acagttacag agagtcaaat ggccattgct attgctcgtg caggcggtct cggagttatc
     7501 cataaaaaca tgtcaattgc tcaacaagca gacgaggttc gtaaggtaaa acgttctgaa
     7561 aatggagtta ttattgatcc gttcttcttg acgcctgaac atacaattgc tgaagcagat
     7621 gagcttatgg gtcgttaccg catcagtggt gttccagttg ttgaaacact tgaaaatcgt
     7681 aaattggttg gtattttgac aaaccgagat cttcgtttta tttcagatta taatcaacca
     7741 atttcaaacc atatgactag tgaaaatctt gttactgctc ctgtgggtac ggatcttgca
     7801 acggctgaga gtattcttca agagcatcgt attgaaaaac ttccgttggt cgatgaagaa
     7861 ggcagtcttt ctggtttgat cactatcaaa gatattgaaa aagttattga gtttccaaat
     7921 gcggctaaag atgagtttgg tcgtcttcta gttgcaggtg cagtaggtgt tacttcagat
     7981 acatttgaac gtgcagaggc tctttttgag gcaggagcgg atgcgattgt tattgatact
     8041 gcacatggtc attctgcagg tgtcttgcgt aaaattgccg agattcgtgc tcatttccca
     8101 gatcggactt tgattgctgg aaatattgct actgctgaag gtgcacgtgc cctttatgaa
     8161 gcgggtgtag acgttgttaa ggttggtatt ggaccaggtt ctatctgtac tactcgtgtg
     8221 attgctggtg ttggtgttcc gcaagtaaca gctatctacg atgctgcagc tgttgcgcgc
     8281 gaatatggta aaacgattat tgctgacggt gggatcaagt attctggaga tattgtaaaa
     8341 gcacttgctg caggtggaaa tgctgttatg cttggatcta tgtttgctgg aactgatgaa
     8401 gctccaggcg aaactgaaat cttccaagga cgtaaattca agacttaccg tggtatggga
     8461 tcaattgctg ctatgaagaa aggttcaagc gaccgttatt tccaaggttc tgtcaatgaa
     8521 gcaaacaagc ttgttccaga aggaattgaa ggtcgtgttg cttataaagg agcggcagct
     8581 gatattgttt tccaaatgat tggtggtatt cgctctggta tgggttactg tggtgcagct
     8641 aaccttaaag aactacacga taatgctcaa tttattgaaa tgtctggtgc tggtttgaaa
     8701 gaaagccatc ctcatgatgt gcaaattact aatgaggcac caaattattc tatgtaaaaa
     8761 acaatgaaaa gaactccagt gaaaacagga gttcttttac aatgttgtca atttccattt
     8821 acagcagctt taccatcctg aatagtgaag atacttagat tttctggcag attttgaaga
     8881 tggtctaagc ttgttgttgt gataaaggtt tggattgatt gagaaatcgt ttctaataat
     8941 tttaactgtc tagtgttgtc aagttcactc atcacatcgt caagcagtaa tataggagat
     9001 tctgtggtaa tgctttccat taattcgatt tctgctaatt ttatcgagag gacgagacta
     9061 cgatgttgac cttggcttcc gaaactagca tccatcccat ttatataaaa agaaatgtca
     9121 tctcgatgag gaccgacacc agtattcttt ttaaataaat ctctggatct acttttttct
     9181 aaagcaattt tgaaagattc ggataagttt tgtttgtcag ttatattgac agaagattga
     9241 taggatattg acaactcttc gatctgatta gagagttcaa aatgtttctt acgcccaaat
     9301 gattctagtt tttttatgaa atctaagcgg tgattcatta cacgacatcc ataatcaact
     9361 agctgatcat ctaacacaga aaggaatgtt tcatctattt tttgagctga ttttaggtaa
     9421 gtgtttcttt gctttaggat gtggttataa ttggttaagt cagataaata gattggctta
     9481 atttgcccaa gttccatatc aatgaatttt cgtcgaatcg aaggtgctcc tttaattagt
     9541 tgtaaatctt caggagcaaa taagacaaca ttcatgtgtc ctacataatc tgaaaggcgt
     9601 gcctgtttta agtgattaac ttttgttaca cgaccttttt gtgttagttc gatttctagg
     9661 ggaatggatc cagttttttt ctgaacgaga cctgaaagat gaagttgttc ctcatcaaaa
     9721 tgaatgagat ttttatctgt tcgagttcga tgactacgcg ttaaggctaa aaaatagata
     9781 gcctctaaca tgtttgtttt tccttgtgca ttacgtccca aaaagacatt caatttagga
     9841 ttaaagtcta ttttcgtttc tttgtaatta cgaaaagtct tgagagatag atgttgtagc
     9901 cacatgatta tctaccaggg aatcgtggag cttgttggct tttaggtgat gaagcaggtt
     9961 tcgatttgtc ttttttaact cccttattca tctttttgac aagtttagcg atccgttctt
    10021 tttcaacttt atcagcttgg tattcatctt gttcttcaga agtaggttgt gtcaacaaga
    10081 tgtcaatgtt catgtcaggg atgtcaactt tatcgccaat acgaagtttt tttccacgac
    10141 ggctctctaa ttccctatta aagtaaacag aatgttcaga gagaaatgat ttaatagctc
    10201 ctccgctatg tgtaattcca agttctttga atagtgcttg gagggtaata aattcttcaa
    10261 ataatttgta ttccataatt cacctcaatc tttggtatta taccatattt tcctaaaaat
    10321 agtgagagag acagcgagaa atgtaagcga tttttatttc aaaagtgtta cagggaacaa
    10381 gaaaatttca ggttttcgtg atataataga agtctgtata taaggaggta aatcatggag
    10441 ttagtgcatg gaatttcaac acattttatc caatcaaaaa agtttaaaac aaacaaaatt
    10501 accgtgcgtt ttaccgctcc attatccctt gatacgattg caggtcacat gttgagtgca
    10561 agtatgctag agactgctaa tcagatgtac cccacttctc aagatttgag gagacacttg
    10621 gccagtctat acggtacaga tatgtcaacc aattgtttca gaagagggca aagccacatt
    10681 atagaattga catttaccta tgttcgtgat gagtttttaa gtaggaaaaa cgtgctaacc
    10741 tctcagattt tggaacttgt aaaagaaact cttttttcac ccgtagtagt tgataatggg
    10801 tttgatccgg ccttatttga aattgagaaa aaacaattgc tagcaagttt agcagctgat
    10861 atggatgatt ctttttattt tgcacataaa gaattggata aattgttttt tcatgatgaa
    10921 cgtcttcaat tggaatatag tgatttacga aatcgtattt tagctgaaac tccacaaagt
    10981 tcttattctt gtttccaaga atttttagcc aatgatcgaa tagatttctt tttcctaggt
    11041 gattttaatg aggttgaaat tcaaaatgta ttagaatcat ttggctttaa aggtcgaaaa
    11101 ggagatgtga aggttcagta ttgtcaacct tattctaata tccttcagga aggtatggtt
    11161 cggaaaaatg tgggacaatc cattttggaa ttaggttatc attactgttc taaatatggt
    11221 gatgagcaac atttacccat gattgtaatg aatggtttac ttggtggatt tgctcactct
    11281 aagctcttta caaatgtccg tgaaaatgct ggattagctt ataccatttc aagtgagctt
    11341 gatttattta gtggattctt gaggatgtat gctggtatca atcgagaaaa tcgtaaccag
    11401 gctcgtaaaa tgatgaataa tcaactgctt gatttaaaaa aaggttattt tacagagttt
    11461 gagttaaatc agaccaagga aatgattcgt tggtcgttgt tactttctca agataatcaa
    11521 tcttcattga ttgaacgtgc ttatcaaaat gccttatttg gaaaatcttc agcagacttt
    11581 aaaagttgga ttgcaaagct tgaacaaatt gacaaagatg ctatttgtag agtagctaat
    11641 aatgtgaaac tacaagcgat ttactttatg gaaggaatag aatgacaaag gttgtttttg
    11701 aagaaaaata ctatccagct gtaaaagaaa tggtttatcg aactcgtttg gccaacggat
    11761 tgacagttgc tcttttgcct aaaaaggaat ttaaagaggt ttacgggagt gtcactgtac
    11821 agtttggttc ggtagatacg tttgtcacag aagttgacgg agatgtaaaa caatatcctg
    11881 gaggaattgc tcattttctt gaacataaat tatttgagag agaagattct agtgatttga
    11941 tgtcggcttt tacgagtcta ggtgcagata gtaatgcctt tacaagcttt acaaaaacaa
    12001 actatctttt ttcagcaacg gattattttt tagaaaattt agatttactt gatgaattgg
    12061 taacatcagc acactttact gaagcttcca ttctgacaga gcaggatatt attcagcaag
    12121 aacgagaaat gtaccaagat gatccagatt cgtgtttatt cttttcaact ttagcgaatt
    12181 tgtatcctgg tacaccttta gcaactgata tagttggaag tgaggagtcc atttcccaaa
    12241 tcaatctaac taatttgcaa gaaaatttta caaagtttta caaacctgta aacatgtctc
    12301 tgtttttagt tggtaatttt gatgtggagc gagtacagga ctattttgaa agcaaagaac
    12361 tgaaagattc agattttcag gaagtagcaa gagaaaagtt gtttttacag cctgtaaagc
    12421 caacagatag tatgagaatg gaagtatctt ctcccaaact agcgattgga gttagaggta
    12481 agcgagaagt ttctgaagcg gattgctatc gacatcatat tttattaaaa ttattgtttg
    12541 caatgatgtt tggttggact tcggatcgtt ttcaaaaatg ttatgaatca ggtaaaattg
    12601 atgcgtcctt atctctggaa gttgaaataa caagtcgctt tcattttgtc atgttgacaa
    12661 tggatacgaa agagccagtt gctttgtctc atcaatttag gaaggctatt cgtaatttta
    12721 caaaggattt agatattaca gaggaacatt tagatattat caaaagagag atgtttggcg
    12781 aatttttcag tagcatgaac tctcttgaat ttattgcaac gcaatatgat gcttttgaaa
    12841 atggtgagat aatttttgat ttgccgaaaa ttttacagga aattacttta gaggatgtcc
    12901 ttgatgctgg acatcattta atagatgatg gtgacatagt tgattttaca atattcccat
    12961 cgtagtaacc tattataata gacactagaa agaagggatg acaagtatga gaaaaaaaac
    13021 aattggagag gttttacgat tagctagaat caatcaggga ttgagtttag atgaattgca
    13081 gaaaaagaca gaaatccagt tagatatgtt ggaagcaatg gaagcagacg atttcgatca
    13141 acttccaagt cctttttaca cgcgttcttt cttgaaaaaa tatgcatggg ctgttgagtt
    13201 agatgaccaa attgttttgg atgcttatga ttctgggagt atgattactt atgaggaagt
    13261 agatgttgat gaagatgagt tgacaggtcg tagacgttca agtaagaaaa agaagaaaaa
    13321 aacatcattt ttacctttat tttattttat cctttttgct ttatcgattt taatttttgt
    13381 gacttattat gtttggaact atattcaaac tcaaccagag gagccttctc tttctaatta
    13441 cagtgtggtt caatcaacaa gttcaactag ctctgttccc cactcctcaa gtagtagttc
    13501 ttctagtata gaatcagcta taagtgtatc aggcgaagga aatcatgtag aaatcgctta
    13561 taagacaagt aaggaaacag ttaaattgca attggcagtt tcagatgtta caagttgggt
    13621 cagtgtttca gaaagcgaac ttgagggcgg tgtaacctta tcgccaaaga agaaaagtgc
    13681 agaagcaaca gttgcaacta aaagtcctgt aacaattacg ttaggtgttg taaaaggtgt
    13741 tgatttgaca gtagataatc agactgttga tttatcgaaa ttaacagctc agactggaca
    13801 aatcactgta acctttacta aaaattaagg aaaaacgaat gaaaaaagaa caaattccca
    13861 atctcttaac aataggtcga attctcttta tacctatttt tatctttatt ttaacgatag
    13921 gaaattcgat agagagtcat atagttgcag ctattatctt tgctgttgcc agtattaccg
    13981 actatttaga tggatattta gctcgtaaat ggaatgtggt cagtaatttt ggtaaatttg
    14041 cagatcctat ggcggataag ttactagtta tgtcggcttt tattatgttg attgagttag
    14101 gtatggctcc ggcttggatt gttgcagtga ttatctgtcg tgagttagct gtgacaggtt
    14161 taaggctttt attggttgaa actggtggaa caattttagc agcagcaatg cctggaaaaa
    14221 ttaaaacttt tagtcagatg tttgccatta ttttcttgct attacattgg actttgcttg
    14281 gtcaagttct actttatgta gccttatttt tcactatcta ctctggctat gactatttca
    14341 agggtagtgc ctatgtattt aaagggacat ttggttcgaa atgaaatcaa taattgatgt
    14401 aaaaaatctt tcttttcgct ataaagaaaa tcagaactac tacgatgtga aggatattac
    14461 gtttcacgtg aaacgtggag aatggctttc gattgtaggg cataatggta gtggtaaatc
    14521 aacgacggtt cgattaattg atggcttact ggaagcagaa tccggagaga ttgtaattga
    14581 tggccaacgt ttgactgagg aaaatgtttg gaatatacgt cgtcaaatcg gtatggtttt
    14641 tcaaaatcca gacaatcaat ttgttggagc gactgttgaa gatgatgttg cttttggttt
    14701 ggaaaatcag ggactttctc gtcaagaaat gaaaaagaga gtggaagaag ctctggcttt
    14761 agttggcatg ttggacttta aaaagagaga gccagcgcgt ctatcaggtg gccaaaagca
    14821 acgtgtggcc attgcaggtg ttgtagccct aagaccagct attttaatct tagatgaagc
    14881 aacgagtatg ttggatcctg aggggcgtag agaacttatt gggacagtaa aaggaattcg
    14941 aaaagactat gatatgacgg tcatttctat tacccatgat ttggaagaag tcgccatgag
    15001 tgatcgcgta ttggtcatga aaaaagggga aattgaatca actagtagtc caagggagct
    15061 tttctctcga aatgatttag atcaaattgg attagacgat ccttttgcta atcaattaaa
    15121 aaaatctttg agccagaatg gctatgattt acctgaaaat tatttgacag aaagtgagct
    15181 agaggataag ctatgggaat tgctctagaa aatgtgaatt ttacatatca agaaggtact
    15241 cccttagctt cagcagcttt gtcggatgtt tctttgacga ttgaagatgg ctcttataca
    15301 gctttaattg ggcacacagg tagtggtaaa tcaactattt tacaactctt aaatggttta
    15361 ttggtgccaa gtcaagggag tgtgagggtt tttgatacct taatcacctc gacttctaaa
    15421 aataaagata ttcgtcaaat tagaaaacag gttggcttgg tatttcagtt tgctgaaaat
    15481 cagatttttg aagaaacggt tttgaaggac gttgcttttg gaccgcaaaa ttttggagtt
    15541 tctgaagaag atgctgtgaa gactgcgcgt gagaaactgg ctctggttgg aattgatgaa
    15601 tcactttttg atcgtagtcc gtttgagctg tcagggggac aaatgagacg tgttgccatt
    15661 gcaggcatac ttgccatgga gccagctata ttagtcttag atgagccaac agctggtcta
    15721 gatcctctag ggagaaaaga gttgatgacc ctgttcaaaa aactccacca gtcagggatg
    15781 accatcgtct tggtaacgca tttgatggat gatgttgctg aatatgcgaa tcaagtctat
    15841 gtaatggaaa agggacgttt agtaaagggg ggcaaaccaa gtgatgtctt tcaagacgtt
    15901 gtttttatgg aagaagttca gttgggagta cctaaaatta cggccttttg taaacgattg
    15961 gctgatagag gcgtgtcatt taaacgatta ccgattaaga tagaggagtt caaggagtcg
    16021 ctaaatggat agtatgattt tagggcgtta tatcccaggg gattcgattg ttcaccgatt
    16081 ggatccacgt agcaaattgc ttgctatgat gctactgatt ttgattgtgt tttgggctaa
    16141 taaccccttg acgaatctga ttctttttat agcgacaggg atatttattg ccttgtcagg
    16201 agtatctctt tcatttttta ttcagggctt gaagtctatg tttttcttga ttgccttcac
    16261 aactattttt caactatttt tcatttctaa tgggaatgtt ttatttgagt tttcgtttgt
    16321 gagaatcacg gattatgctt tgcaacaagc tgggattatt ttttgtcgct ttgtattgat
    16381 tattttcttt tcaactttgt taaccttaac aaccatgcct ttaagtttgg catcagctgt
    16441 cgaagcttta ttagcacctt taaagcgtgt gaaagttcca gttcatgaaa ttggattgat
    16501 gctgtctata agtttgcgtt ttgtcccaac cttgatggat gatacgactc ggattatgaa
    16561 tgcacagaaa gctcgtggag tggattttgg agaaggaagc atcgttcaaa aagtaaaggc
    16621 gatgattccc attttgattc ctctttttgc gacaagttta aaacgtgcag attccttggc
    16681 tatcgctatg gaagcgcgtg gctatcaggg tggaaaaggc agaagtcaat accgacaatt
    16741 gaaatggact ctaaaggata cgctgaccat tcttgttatt ctcgtacttg gttgttgttt
    16801 atttttctta aaatcttagt agctttcagg aattgataaa aagttactgt aacagttttt
    16861 gatataaagg tagggatatg aaccgtttta aaaaatcaaa atatgtcatt attgtttttg
    16921 tcactgttct gcttgtgtca gctctcttag cgacgactta ttcaagtaca attgtgacaa
    16981 aattaggaga tggaatctca ttggttgata gagttgtaca aaaacctttt cagtggtttg
    17041 attctgtcaa atcagatttg gctcatttga cacgaacata taatgaaaat gaaagtttga
    17101 agaaacagct ttaccaatta gaagttaaat caaatgaggt ggaaagttta aagacagaaa
    17161 atgaacaact gcgccaattg cttgatatga agtctaaatt gcaagccaca aagactttag
    17221 cagcagatgt tattatgcgt tctccggtat cttggaagca ggagttgacc ttagatgcag
    17281 gtagatcaaa aggtgcttct gagaacatgt tagctattgc aaatggtggc ttgattggga
    17341 gtgtttcaaa agtagaggag aactctacta tagtcaacct tctgacaaat acggaaaatg
    17401 ctgataagat ttctgttaaa attcaacatg gctctactac aatttatgga attattattg
    17461 gctatgacaa ggaaaatgac gttcttaaaa ttagccaatt aaatagtaat agcgatatta
    17521 gtgcgggaga taaggtgact actggtggat taggaaactt taacgttgct gatattcctg
    17581 ttggtgaagt ggttgccaca acgcatagta cagactattt gacacgagaa gtaactgtta
    17641 aattgagtgc agatactcat aatgtagatg tgatagaatt agtggggaat tcataatgag
    17701 acagttgaag cgagttggag tatttttatt gcttcctttc tttgttctaa ttgacgccca
    17761 tattagccag cttctgggct catttttccc ccatgtacat ttggctagtc attttctttt
    17821 tctatttctc ttatttgaga cgatagaagt atcagagtat ctctacctag tctattgttt
    17881 tgttataggc ttgatttatg atgtttactt tttccatcta atagggatta caactctctt
    17941 atttatctta ttgggagcct tccttcataa attgaatagt gttattttgt tgaatcgttg
    18001 gacaagaatg ctagctatga ttgtgctgac attcctgttt gaaatgggta gttatctttt
    18061 ggcttttatg gtagggttga cagtagatag catgtcgatt tttatagtct atagcttggt
    18121 accgacgatg attttaaatt ttttatggat tactgttttt caatttattt ttgaaaaata
    18181 ttatctataa gaacgacata taaatgtaac aaaggcgtaa tatttattag gccttttttt
    18241 ggtatactag tattgtcttt aaaagaagga gtatctacgt aatatgaaga aaaaaatctt
    18301 agcgtcactt ttattaagta cagtaatggt ttctcaagta gctgttttaa caactgcgca
    18361 tgcagaaacg actgatgaca aaattgctgc tcaagataat aaaattagta acttaacagc
    18421 acaacaacaa gaagcccaaa aacaagttga ccaaattcag gagcaagtat cagctattca
    18481 agctgagcag tctaacttgc aagctgaaaa tgatagatta caagcagaat ctaagaaact
    18541 cgagggtgag attacagaac tttctaaaaa cattgtttct cgtaaccaat cgttggaaaa
    18601 acaagctcgt agtgctcaaa caaatggagc cgtaactagc tatatcaata ccattgtaaa
    18661 ctcaaaatca attacagaag ctatttcacg tgttgctgca atgagtgaaa tcgtatctgc
    18721 aaacaacaaa atgttagaac aacaaaaggc agataaaaaa gctatttctg aaaaacaagt
    18781 agcaaataat gatgctatca atactgtaat tgctaatcaa caaaaattgg ctgatgatgc
    18841 tcaagcattg actacgaaac aggcagaact aaaagctgct gaattaagtc ttgctgctga
    18901 gaaagcgaca gctgaagggg aaaaagcaag tctattagag caaaaagcag cagctgaggc
    18961 agaggctcgt gcagctgcgg tagcagaagc agcttataaa gaaaaacgag ctagccaaca
    19021 acaatcagta cttgcttcag caaacactaa cttaacagct caagtgcaag cagtatctga
    19081 atctgcagca gcacctgtcc gtgcaaaagt tcgtccaaca tacagtacaa acgcttcaag
    19141 ttatccaatt ggagaatgta catggggagt aaaaacattg gcaccttggg ctggagacta
    19201 ctggggtaat ggagcacagt gggctacaag tgcagcagca gcaggtttcc gtacaggttc
    19261 aacacctcaa gttggagcaa ttgcatgttg gaatgatggt ggatatggtc acgtagcggt
    19321 tgttacagct gttgaatcaa caacacgtat ccaagtatca gaatcaaatt atgcaggtaa
    19381 tcgtacaatt ggaaatcacc gtggatggtt caatccaaca acaacttctg aaggttttgt
    19441 tacatatatt tatgcagatt aatttacaga gggactcgaa tagagccctc ttttcaggtt
    19501 ttaccgtgac aatccctatt aaaaattata tcaaaatagc ttgaaaatat tggaaaagta
    19561 tggtagaatg aaaattgtcg tgtgaacgat aatactcatt cttgatgaat tgtgaagcag
    19621 ttgcccttgg gtcgttttgc gagttgaagt caagaagagg aaaaaaacaa aaaggagaaa
    19681 tactcatggc agtaatttca atgaaacaac ttcttgaggc tggtgtacac tttggtcacc
    19741 aaactcgtcg ctggaatcct aagatggcta agtacatctt tactgaacgt aacggaatcc
    19801 acgttatcga cttgcaacaa actgtaaaat acgctgacca agcatacgac ttcatgcgtg
    19861 atgcagcagc taacgatgca gttgtattgt tcgttggtac taagaaacaa gcagctgatg
    19921 cagttgctga agaagcagta cgttcaggtc aatacttcat caaccaccgt tggttgggtg
    19981 gaactcttac aaactgggga acaatccaaa aacgtatcgc tcgtttgaaa gaaattaaac
    20041 gtatggaaga agatggaact ttcgaagttc ttcctaagaa agaagttgca cttcttaaca
    20101 aacaacgtgc gcgtcttgaa aaattcttgg gcggtatcga agatatgcct cgtatcccag
    20161 atgtgatgta cgtagttgac ccacataaag agcaaatcgc tgttaaagaa gctaaaaaat
    20221 tgggaatccc agttgtagcg atggttgaca ccaatactga tccagatgat atcgatgtaa
    20281 tcatcccagc taacgatgac gctatccgtg ctgttaaatt gatcacagct aaattggctg
    20341 acgctattat cgaaggacgt caaggtgagg atgcagtagc agttgaagca gaatttgcag
    20401 ctttagaaac tcaagcagat tcaattgaag aaatcgttga agttgtagaa ggcgacaacg
    20461 cttaatttat acaaatagta attacctagg agggcggggc ttagcccggc tctcctattt
    20521 tcaaaaaata taggagaatt aaaatggcag aaattacagc taaacttgta aaagagttgc
    20581 gtgaaaaatc tggtgccggt gttatggacg ctaaaaaagc gcttgtagaa acagacggtg
    20641 acatcgaaaa agcgattgaa ttgcttcgtg aaaaaggtat ggctaaggca gctaagaaag
    20701 ctgaccgtgt tgctgcagaa ggtttgactg gtgtttatgt taacggtaat gttgcagcag
    20761 ttattgaagt aaacgctgaa actgacttcg ttgcaaaaaa cgctcaattc gttgaattgg
    20821 taaatactac agctaaagtc attgctgaag gaaaacctgc taacaacgaa gaagcacttg
    20881 ctttgataat gccttcaggt gaaactcttg aagctgcata cgtatctgca acagcaacta
    20941 tcggagagaa aatctcattc cgtcgctttg cattgattga aaaaacagac gcacaacact
    21001 ttggagcata ccaacataac ggtggacgta tcggtgttat ttcagttgtt gaaggtggag
    21061 acgaagcact tgctaaacaa ttgtcaatgc acatcgcagc gatgaaacca acagttcttt
    21121 cttacaaaga attggatgag caattcgtta aagatgagtt ggcacaattg aatcacgtta
    21181 tcgaccaaga caacgaaagc cgtgcaatgg ttaataaacc agctcttcca cacttgaagt
    21241 atggatcaaa agctcaatta actgatgatg ttattgctca agctgaagct gacatcaaag
    21301 ctgaattggc tgcagaaggc aaaccagaaa aaatctggga caaaattatt ccaggtaaaa
    21361 tggatcgctt catgcttgat aacactaaag ttgaccaagc ttacacactt cttgcacaag
    21421 tttacatcat ggatgacagc aagacagttg aagcatacct tgaatcagtt aacgcttcgg
    21481 tagttgagtt tgctcgcttt gaagttggtg aaggtatcga gaaagctgca aacgacttcg
    21541 aagctgaagt tgcagctaca atggcagcag ccttgaataa ctaataatag aaaggaagca
    21601 aattgcttcc ttttttgtat agggcttaga tttttcctga aattgttttt tccccagtct
    21661 cactcgatta gaataattaa aacaaaaagc cctataataa gggctaagtg catttaggag
    21721 actatacttc aaattcatag taagcgcgtc ataacaaggt atctatccta attccccaac
    21781 ttccagtata ccagataaaa aataattatc ggaggaaagg tattgtacta tgataattca
    21841 actaagtgat ttaggtcaag ttcaccttgt ttgtggcaag acagatatga ggcagggaat
    21901 agactcatta gcctatgtag ttaaaaccca ctttgaattg gatcctttct ccgatcaagt
    21961 ctttctcttt tgtggtggac gtaaagaccg ctttaaagtt ctttactggg atggtcaagg
    22021 attttggata ttatataaac gctttgagaa cggaaaactg acttggtcca gtacagaaaa
    22081 ggatgtcaaa gctctcgcac ctgaacaagt agattggctg atgaagggct tttctatcac
    22141 tccccaaata tagtggattt aaactagaat agtacacctc tgcttctaaa acattgttag
    22201 aaatcgattt gactatcctg ttcttatttc attttactat aaattcatca gaaagtcgtg
    22261 atttctattg aaatgaggac tttcttttta tactcatctg ttttcaaaaa gcattctagt
    22321 ccgtctccga ttaacgatgg actttatcac ctccttctcc agtccttgta tgacatcttc
    22381 caaagttcga aaggctttat tcttaaatcc acgttta
//
LOCUS       DAVHEW010000023        22262 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVHEW010000023 DAVHEW010000000
VERSION     DAVHEW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.023"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            98..2320
                     /gene="recJ"
                     /locus_tag="VVL53_000838"
     CDS             98..2320
                     /gene="recJ"
                     /locus_tag="VVL53_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU4183036.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2495..4156
                     /locus_tag="VVL53_000839"
     CDS             2495..4156
                     /locus_tag="VVL53_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU4183037.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4225..5004
                     /locus_tag="VVL53_000840"
     CDS             4225..5004
                     /locus_tag="VVL53_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU4183038.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5116..6336
                     /gene="murM"
                     /locus_tag="VVL53_000841"
     CDS             5116..6336
                     /gene="murM"
                     /locus_tag="VVL53_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU4183039.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRVV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6341..7573
                     /gene="murN"
                     /locus_tag="VVL53_000842"
     CDS             6341..7573
                     /gene="murN"
                     /locus_tag="VVL53_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU4183040.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7652..8353
                     /locus_tag="VVL53_000843"
     CDS             7652..8353
                     /locus_tag="VVL53_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU4183041.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8393..10240
                     /gene="uvrC"
                     /locus_tag="VVL53_000844"
     CDS             8393..10240
                     /gene="uvrC"
                     /locus_tag="VVL53_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU4183042.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10230..11063
                     /locus_tag="VVL53_000845"
                     /pseudo
     CDS             10230..11063
                     /locus_tag="VVL53_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11247..12047)
                     /locus_tag="VVL53_000846"
     CDS             complement(11247..12047)
                     /locus_tag="VVL53_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4183043.1"
                     /translation="MKKFSLLLAILPFLVACGNQVTPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12218..12823
                     /locus_tag="VVL53_000847"
     CDS             12218..12823
                     /locus_tag="VVL53_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU4183044.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12836..14236
                     /gene="pepV"
                     /locus_tag="VVL53_000848"
     CDS             12836..14236
                     /gene="pepV"
                     /locus_tag="VVL53_000848"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU4183045.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14290..14868
                     /locus_tag="VVL53_000849"
     CDS             14290..14868
                     /locus_tag="VVL53_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU4183046.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15028..15180
                     /locus_tag="VVL53_000850"
     CDS             15028..15180
                     /locus_tag="VVL53_000850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4183047.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15223..15315
                     /locus_tag="VVL53_000851"
     CDS             15223..15315
                     /locus_tag="VVL53_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4183048.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15486..16811
                     /gene="brnQ"
                     /locus_tag="VVL53_000852"
     CDS             15486..16811
                     /gene="brnQ"
                     /locus_tag="VVL53_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU4183049.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIANVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            17016..18053
                     /locus_tag="VVL53_000853"
     CDS             17016..18053
                     /locus_tag="VVL53_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU4183050.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            18055..18525
                     /locus_tag="VVL53_000854"
     CDS             18055..18525
                     /locus_tag="VVL53_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU4183051.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLA
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            18528..19244
                     /locus_tag="VVL53_000855"
     CDS             18528..19244
                     /locus_tag="VVL53_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU4183052.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            complement(19284..19421)
                     /locus_tag="VVL53_000856"
     CDS             complement(19284..19421)
                     /locus_tag="VVL53_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183053.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDVVS
                     R"
     gene            19553..19978
                     /gene="rplK"
                     /locus_tag="VVL53_000857"
     CDS             19553..19978
                     /gene="rplK"
                     /locus_tag="VVL53_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU4183054.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            20186..20875
                     /gene="rplA"
                     /locus_tag="VVL53_000858"
     CDS             20186..20875
                     /gene="rplA"
                     /locus_tag="VVL53_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU4183055.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(21584..21664)
                     /locus_tag="VVL53_000859"
                     /pseudo
     CDS             complement(21584..21664)
                     /locus_tag="VVL53_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            21672..22034
                     /locus_tag="VVL53_000860"
     CDS             21672..22034
                     /locus_tag="VVL53_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU4183056.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         6699 a         4121 c         5100 g         6342 t
ORIGIN      
        1 tactataaaa tgttataaaa aagcaatctg gatagagaaa acgtctaaat catgttataa
       61 tgaagcaata gaattcttag aaagagtgga tgtctttttg ataacaccta cttatgaatg
      121 gcagtttgcc ctgcaggtag aagatgcgga ttttacaaag atagccaaga aggctggact
      181 gggtcctgag gtggctcggt tattgtttga gagagggatt caggaccaag aaagtctgaa
      241 gaagttttta gaaccttcct tggaggactt acatgatgct tatctgctcc atgatatgga
      301 caaggcagtg gagcggattc gtcaggctat tgaagaaggg gaaaatattc tcgtttatgg
      361 agactatgat gcggatggca tgacttcggc ttctattgtg aaggaaagtt tggaacaact
      421 tggtgctgag tgccgagttt acctgccaaa tcgttttacc gatggctatg gccctaatgc
      481 tagtgtttat aaatacttta tcgagcaaga aggaatttcc ttgattgtga cggtggacaa
      541 tggggttgct ggtcatgagg ctattgcatt ggctcagtct atgggagtag atgtcattgt
      601 gacagaccat cattccatgc ctgaaaccct gccagatgct tatgctattg tccatcctga
      661 acatccagat gcggattatc cttttaaata tttggctggt tgtggagttg ctttcaagtt
      721 ggcttgtgcc ctgttagaag aagtgcaagt ggaattgctt gatttggtcg ctattggaac
      781 tattgcagat atggtgagtc tgacggatga aaatcgtatc ttagttcaat atggtctgga
      841 aatgttgggt catacccagc gcattggtct gcaagaaatg ctggacatgg ctgggattgc
      901 tgccaacgaa gtaacagaag aaacggttgg tttccagatt gctcctcgtt tgaatgcctt
      961 gggccgcttg gatgatccca atcctgccat tgatttgttg actggatttg atgatgagga
     1021 agcgcatgag attgccctta tgattcacca gaaaaacgaa gagcgcaagg aaatcgttca
     1081 gtctatctat gaagaagcca agaccatggt ggatcctgag aagaaggttc aggtcttggc
     1141 caaggaaggc tggaatcctg gggttctagg aatcgtggct ggtcgtttat tggaagaatt
     1201 gggacagaca gtcattgttc ttaatataga agacggtcgt gccaagggca gtgctcgtag
     1261 tgtggaagcg gtcgatattt ttgaagctct ggatccctat cgagacctct tcatcgcctt
     1321 tggaggtcat gcaggtgcag cgggtatgac gctggaagtt gagcaactct cagatttatc
     1381 tcaggttttg gaagattatg ttcgtgaaaa aggtgcagat gctggtggca agaataagtt
     1441 aaacctagat gaagagttgg atttggaggc acttagcttg gaaacggtca aaagttttga
     1501 acgtttagct ccttttggaa tggataatca gaaacctatt ttttatatca agaattttca
     1561 ggtcgaaagt gctcgtacta tgggggcagg taatgcccat ctaaagctga aaatttccaa
     1621 gggtgaggcg agttttgaag tggtagcctt tggtcaaggc agatgggcga cagagttttc
     1681 tcaaaccaag aatctagagt tagcggttaa attgtctgtc aaccaatgga atggccaaac
     1741 tgccctccag ttgatgatgg tggatgcgcg agtggaaggt gttcagcttt ttaacattcg
     1801 tggaaaaaat gcagtcttgc cagaaggtgt tccagtcttg gattttcctg gagaactgcc
     1861 aaatcttgcg gctagtgaag ctgttgtcgt aaaaaacatt ccagaggata ttactcagct
     1921 gaagaccatt tttcaggaac agcatttctc tgctgtctat ttcaaaaatg atattgacaa
     1981 ggcttattat ctgacaggtt atgggactag agatcagttt gccaaattgt acaagactat
     2041 ttaccagttc ccagagtttg atattcgcta caagctgaaa gatttggctg catatcttaa
     2101 tattcaacaa atcttgctgg tcaagatgat tcaagtattt gaagaactag gctttgtgac
     2161 gataaaagat ggtgtgatga cagtcaataa agaggcgcca aagcgggaga taggagaaag
     2221 tcaaatttac caaaatctca aacaaaccgt taaagaccaa gaaatgatgg cgctgggtac
     2281 ggtgcaagaa atttatgatt ttttgatgga aaaagagtag aagttaggaa agagttggga
     2341 aatcaactct tttttgaaaa cagaccttca ttttgaaaat catcaaaaaa atggtataat
     2401 ggtaggaaaa gattcggctg aaagtatcag aacttttaga ataagagggt agaattgccc
     2461 tataatcaag ataaactaag attttggagg aaaaatgagt aatatcagtt taacaacact
     2521 tggtggtgtg cgtgagaatg gaaaaaatat gtacattgct gaaattggag agtccatttt
     2581 tgttttgaat gtagggttaa aatatcctga aaatgaacaa ttaggggtcg atgtggtgat
     2641 tccaaacatg gattaccttt ttgaaaatag cgaccgtatt gctggggttt tcttgaccca
     2701 cgggcatgcg gatgccattg gtgctctacc ttatctcttg gcagaggcta aagttcctgt
     2761 atttgggtct gagttgacca ttgagttggc aaagctcttt gtcaaaggaa atgatgccgt
     2821 taagaaattt aatgatttcc atgtcattga tgagaatacg gagattgatt ttggtgggac
     2881 agtggtttcc ttcttcccta cgacttactc cgttccagag agtctgggaa ttgtcttgaa
     2941 gacatcggaa ggaagcatcg tttatacagg tgacttcaaa tttgaccaaa cggctagtga
     3001 atcttatgca actgattttg ctcgtttggc agagattggt cgtgacggcg tcctggctct
     3061 cctcagtgat tcggccaatg cagacagcaa tattcaggtg gctagtgaaa gtgaagttag
     3121 ggatgaaatt acccaaacta ttgctgactg ggaaggtcgt atcatcgttg cagctgtttc
     3181 cagtaatctt tctcgtattc agcagatttt tgacgctgcg gataaaacag gtcgacgtat
     3241 cgtcttgaca ggatttgata ttgaaaatat cgtccgcaca gcgattcgtc ttaagaagtt
     3301 gtctttagcc aacgaaattc tcttgattaa gcctaaagat atgtctcgct ttgaagacca
     3361 tgagttgatt attcttgaga caggtcgtat gggtgaacct atcaatggac ttcgtaagat
     3421 gtcgattggt cgccatcgtt atgtagaaat caaggatggg gacctggtct atattgctac
     3481 ggctccgtct attgctaaag aagcctttgt tgcgcgtgtg gaaaatatga tttatcaggc
     3541 aggtggggtt gtcaaattga ttacccaaag tttacatgta tcagggcacg gaaatgtgcg
     3601 tgatttgcag ctgatgatca atcttttgca acctaagtac ctcttccctg tccaagggga
     3661 gtatcgtgag ttggatgctc acgctaaggc tgccatggca gttgggatgt tgccagaacg
     3721 catcttcatt cctaaaaagg ggacgaccat ggcttacgag aatggagact ttgttccagc
     3781 tggatcggtt tcagcaggag atatcttgat tgatgggaat gccattggtg atgttggaaa
     3841 tgttgttctt cgtgaccgta aggtcttgtc agaggatgga attttcatcg tggctattac
     3901 agtcaaccgt cgtgagaaga aaattgtggc tagggctcgt gttcacacgc gtggatttgt
     3961 ttatctcaag aagagtcgcg atattctccg tgaaagttca gaattgatta accaaacggt
     4021 agaagagtat cttcaaggag atgactttga ctgggcagat ctcaaaggta aggttcgtga
     4081 caatctgacc aagtacctct ttgatcaaac caagcgtcgc ccagccattt taccagtagt
     4141 catggaagca aaataatcgt tgaaataaac agagagaaag tcgagtttcg gctttttctt
     4201 atagaaaaat agaaggagaa aatcatggca gtaatgaaaa tcgagtatta ctcacaagta
     4261 ttggatatgg agtggggggt gaatgtcctc taccctgatg ccaatcgagt ggaagaacca
     4321 gagtgtgaag atattcccgt cttgtacctt ttgcacggga tgtctggaaa tcataatagt
     4381 tggcttaagc ggaccaatgt agaacgcttg cttcgaggaa ctaatctcat cgttgttatg
     4441 cccaatacca gcaatggttg gtacaccgat acccagtatg gttttgacta ctacacggct
     4501 ctagcagagg aattgccaca ggttctgaaa cgcttcttcc ctaatatgac gagcaagcgt
     4561 gaaaagacct ttatcgctgg tctttctatg ggaggctacg gctgcttcaa actggctctt
     4621 acgacaaatc gtttttctca tgcagctagt ttttcaggtg ccctcagctt tcaaaacttt
     4681 tctcctgaaa gtcaaaatct gggaagtcca gcctactgga gaggtgtttt tggagagatt
     4741 agagactgga caactagtcc ctattctctt gaaagtctgg ctaaaaaatc ggataaaaag
     4801 accaaacttt gggcgtggtg tggcgaacag gatttcttgt acgaagccaa taatctcgca
     4861 gtgaaaaatc tcaaaaaact aggttttgat gtgacctata gccatagcgc tggaactcac
     4921 gagtggtact actgggaaaa acaattggaa gtttttttaa caaccctacc aattgatttc
     4981 aaattagaag agagactgac ttagtttgaa cttcagcata gggggagtag aactaaaata
     5041 aaatatgttt tcactagact tttcaaacga aagtagtaga atagtaataa aatactggag
     5101 gaaagagagt aggaaatgta ccgttatcaa attggcattc ccacattaga atatgatcag
     5161 tttgtcaaag aacatgaatt agccaatgta ttacaaagta gtgcttggga ggaagttaag
     5221 tctaattggc aacatgagaa gtttggtgtt tacagggaag aaaaattact ggcgacagct
     5281 agtattttga ttagaactct tccgctaggc tataaaatgt tttacatccc aagaggacct
     5341 atattggatt atggggataa agaactcttg aattttgcca ttcagtctat taagtcctat
     5401 gctcgcagta agagagtggt ttttgtgact tttgacccaa gtatttgcct atctcaaagt
     5461 ttaatcaatc aggaaaagac agaatttcct gaaaatctgg ctattattga tagtttgcaa
     5521 caaatgggag taaggtggtc aggaaaaacg gaggaaatgg gagacaccat tcaacctcgt
     5581 attcaggcga aaatatacaa ggaaaatttt gaagaagata aactttccaa gtcaacaaaa
     5641 caggctattc gaacagcacg aaacaaaggg cttgagattc aatatggtgg actggaacta
     5701 ttagattcat tttcggagtt gatgaaaaaa actgagaagc gaaaagagat tcatttgagg
     5761 aatgaagcct attataaaaa attgttagat aattttaagg acaaggccta tatcaccttg
     5821 gccaccttgg atgtttctaa acgttcgcaa gaattagaag aacagttagc gaaaaataga
     5881 gccttggaag agacctttac tgagtcgact cgaacttcaa aagtagaagc gcagaagaag
     5941 gaaaaagaac gtttgttaga ggaattgacc ttcttgcagg aatatataga tgtaggtcaa
     6001 gcgagagttc ctttagcggc tactttgagt ttggaatttg gtactacctc tgtcaatata
     6061 tatgctggta tggatgatga ttttaaacgt tacaatgcac caattttaac atggtatgaa
     6121 acggctcgct atgcctttga gcgaggtatg gtctggcaaa atttaggtgg tgttgaaaac
     6181 tctctcaatg gtggacttta tcattttaag gaaaaattta atccaacgat tgaagaatac
     6241 ttgggtgaat ttacaatgcc cactcatcct ctctatcctc tgttaagact tgctcttgat
     6301 ttccgtaaaa cattaagaaa aaaacataga aagtaagtat atggcactaa caacactcac
     6361 gaaagaagag tttcagactt attctgatca ggtttcttct cgttccttta tgcaatctgt
     6421 ccagatgggg gatttgctag aaaaaagagg ggctcgaatt gtttatcttg ctttgaaaca
     6481 agaaggagaa attcaagttg cagctctggt ttatagcttg cccatgctgg gtggtctgca
     6541 tatggaactc aattcggggc cgatttatac ccaacaagat gctcttccag ttttttatgc
     6601 agagttaaaa gaatatgcca agcaaaatgg tgtattagag ttgcttgtaa aaccttatga
     6661 aacttatcaa acttttgata gccaaggtaa tccaatagat gctgagaaaa aaagtattat
     6721 tcaaggtttg actgatttag gttatcaatt tgatggctta acaacaggtt acccaggtgg
     6781 agaaccagat tggttatact ataaagattt aactgaatta actgaaaaga gtttgcttaa
     6841 aagttttagc aaaaagggta aacccttggt gaaaaaggct gaaacctttg gcattcggtt
     6901 gaaaaagtta aaacgtgaag aactatcgat ttttaagaat ataacaaaag aaacctctga
     6961 acgtagagaa tatagtgata aaagtttaga atattatgag catttttatg atacttttgg
     7021 agaacaagcg gagtttctca tagcaagctt aaatttttcg gactatatga gcaaattgca
     7081 aggtgaacaa agtaaactag aagaaaactt ggacaagttg cgacttgatt tgagtaaaaa
     7141 tcctcattct gagaaaaaac aaaatcaact gagagaatat tctagtcaat ttgaaacgtt
     7201 tgaagttcga aaagcagaag cgcgagactt gattgaaaaa tatggagaag aagatattgt
     7261 tttagctggg agtttatttg tttatatgcc tcaggaaacg acttatctct ttagtggttc
     7321 ctacactgag tttaataagt tctatgcccc tgcactgctt caaaaatatg ttatgttgga
     7381 aagcataaaa cgtggaatac ctaaatacaa cttcctaggc attcaaggga tttttgatgg
     7441 aagtgatggt gttttgcgtt ttaaacagaa ttttaatggc tatattgtac gcaaagcagg
     7501 tactttccgt taccatccat cgcctttaaa atacaaagct atccagttac tcaaaaaaat
     7561 agtaggacgt taagatgaaa aagtcagtat ttagatttct tttagcttct tttagtaaaa
     7621 taattcttat ttgctagaaa ggtggagaga catgcgctgg ctttttcgtt tgataggggc
     7681 tttcttttct tttgtgtggc gtttgttttg gcgtctggtt tggatagttg tgctcttatg
     7741 tgtgcttgct ttcggacttc tctggtatct gaacggagat tttcaaggag cgctaaagca
     7801 agcagaacgg tcagtaaaaa ttggtcaaca aagtattgac caatgggaga aaacagggca
     7861 actgcctaag ttaagccaga cagatagtca ccagcattct gaaggaaggt gggcacaggc
     7921 ctctgctcgt atttacctgg atccgcagat ggattcacgc tttcaagagg cttatttaga
     7981 agcaatccag aactggaatc aaactggtgc ttttaacttt gaactcgtga ctgagtctag
     8041 taaggcggat attacggcta cggagatgaa cgacggaggc actcctgtgg caggagaggc
     8101 ggaaagtcaa actaatctct taacagggca attcttgtcc gtaacggtgc ggttgaatca
     8161 ttattatttg tccaatccat actatggcta ctcctatgaa cgccttgtcc atacggcaga
     8221 acatgagtta ggtcatgcga ttggcttgga ccatacagat gagaagtctg tcatgcaacc
     8281 agcaggttcc ttttatggta tccaggaaga ggatgttgca aacctccgaa aaatatatga
     8341 gactagtgag tagggtacta tctttcccta ctttttttgc tataatggaa ctatgaacaa
     8401 cttgattaaa tcaaaactag agctcttgcc gaccagccct ggttgctaca ttcataagga
     8461 taaaaatggc accattatct atgtaggaaa ggctaaaaat ctgcgtaatc gagtacggtc
     8521 ctattttcgt ggaagtcatg ataccaagac agaggctctg gtgtctgaaa ttgtggattt
     8581 tgaatttatt gttacggagt ctaatattga ggcacttctc ctagaaatca acctgatcaa
     8641 ggaaaacaag cccaagtaca atatcatgct caaggatgac aagtcctatc ctttcatcaa
     8701 aatcaccaat gagcgctatc cacgcttgat tatcactcgt caggtcaaaa aggacggagg
     8761 tctttatttt ggaccctatc ccgatgtggg ggcagccaat gaaatcaagc ggttgctgga
     8821 tcggatattc ccttttcgta agtgtaccaa cccgccctct aaggtctgtt tttattacca
     8881 tatcggtcaa tgcatggccc acaccatctg taagaaggat gagatttatt tcaagtctat
     8941 ggcccaggag gtttctgatt ttctgaaagg gcaggataac aaaatcattg atgagctaaa
     9001 gggcaaaatg gcagcggcag cccagactat ggagtttgaa cgtgcggcgg aataccgtga
     9061 cctgattcag gctattggaa cgcttcgaac caagcaacgg gtcatggcta aggatctcca
     9121 aaatcgcgat gtctttggct actatgtgga taagggctgg atgtgtgtgc aggttttctt
     9181 tgtccgtcag ggtaagctca tcgagcgcga tgtcaatctc ttcccctact tcaatgatcc
     9241 agatgaggat tttttgacct atgtaggaca attctatcaa gaaaaatccc atctagttcc
     9301 caatgaggta ctgattccgc aagatattga cgaagaggct gtcaaggcct tggtggattc
     9361 caagattctt aagcctcaac gtggagagaa aaagcagctg gtcaatttag ctataaaaaa
     9421 tgcccgtgtt agcctagagc agaagttcaa cctgctagag aaatctgtcg agaagaccca
     9481 aggagctatt gaaaatctag gacgcttgct tcaaatccca accccagttc gcatcgagtc
     9541 cttcgataac tccaacatca tgggaactag tcctgtttca gccatggtgg tctttgtcaa
     9601 cggcaagcct agtaagaagg attaccgtaa gtataaaatc aagaccgtag tcgggccaga
     9661 cgactatgcc agtatgcgag aggtcattcg cagacgctat ggtcgagtac agcgtgaggc
     9721 tttgactcct ccagatttga ttgtgattga tggggggcaa ggtcaagtca atattgctaa
     9781 gcaagtcatc caagaagaac tgggcttgga tattccaatt gcaggtctgc aaaagaatga
     9841 taagcaccaa acccatgaat tgctctttgg agatccgctg gaagtggtgg acttgtctcg
     9901 caattctcag gaatttttcc tcctccaacg cattcaagat gaggtccacc gctttgccat
     9961 taccttccac cgccaactgc gctccaaaaa ttctttctca tctcaattgg atgggattga
    10021 tggtctggga cctaaacgca agcaaaatct catgaggcac ttcaagtctc tgaccaaaat
    10081 caaggaagcc agtgtggatg agattgtcga agttggagta ccaagagcag tagcagaggc
    10141 tgtgcaaacc aagttgaacc cgcaggaaac agaaatcttg cttcaagtgg cggaagaaag
    10201 agtagattac caaacggaag gaaaccacaa taaaccataa aatcgcaatt ttatcagatg
    10261 ttcatggcaa tgcgacggcg ctagaagcag tgattgcaga tgctaaaact caaggagcca
    10321 gtgaatattg gctcatggga gacattttcc tccctggtcc aggtgcaaat gacttggtcg
    10381 ctctgttaaa ggaacttcct atcacggcaa gtgttcgagg caattgggat gattgtgtcc
    10441 ttgaggcttt agatgggcaa tatggcttag aagacccaca ggaagttcag ctcttgcgta
    10501 tgacacagta tttgatggag cgaatggatc ctgcaacgat tgtctggcta cgaagcttgc
    10561 ctttgctgga aaagaaagaa attgacggat tgcgcttttc tatctctcat aatttacctg
    10621 acaaaaacta tggtggtgac ttgctagttg agaatgatac agagaaattt gaccaactgc
    10681 tagatgcgga aacggacgtg gcagtttatg gtcatgttca caagtagttg cttcgttatg
    10741 gtagtcaagg gcaacaaatc atcaatccag ggtcgattgg catgccctat tttaattggg
    10801 aggcgttaaa aaatcaccgt gcccagtatg ccgtgataga ggttgaagat ggggaattgg
    10861 taaatatcct atttcgtaaa gtcgcttatg attacgaggc tgagttagaa tttgccaagt
    10921 ccaaggggtt tccctttatc gaaatgtatg aagaactacg tcgtgaagat aactatcaga
    10981 ggcacaatct agaactatta gcaagcttaa tagaaaagca taggtatgta gaggatgtga
    11041 agaatttttt tgattttttg taagagtttc ctaaaataac caatgcaaac taaaaaagcg
    11101 attggctgat cccatcgctt ttaatatatc ttatactcaa tatgaaaatc aaagagcaaa
    11161 ctaggaagct agccgtaggt tgctcaaagc acagctttga ggttgcagat aaagctgacg
    11221 tggtttgaag agattttcga agagtgttat tgtaactgag attgatctgg gaggtaagaa
    11281 ccacctagat aggtattgct gagtttttca agggttccgt cttgatagag ttctttgagc
    11341 gctttatcaa attgctcttt aaactctttt tggtcgcttg agaaaatgat ataattgctg
    11401 gggctatctg cagaaggtaa atcaacgact gagaggtcta aaccacggtc cttgataatc
    11461 ttttgaacgg ataccttgtc aaaaactagg aaatcaaact ctccgttagc aaggtctagg
    11521 attcgtttac caatatcctc accagaaaaa ttaattgtag cgggattatc agtgtgtttc
    11581 tgattccagt tattgatgaa ttgagcgtta gaagttccgg tatcctcttg tgttgtttta
    11641 ccagcgatct ggtcaagaga agtcaaagga tttttcttgt tgctgacaag gacgagggga
    11701 ttgttggaaa tcggaagcga gtaaaggtat ttttcagcac gctcttttgt gtaactcaaa
    11761 ttattggccg cagcctgata gtgaccagaa tcaagtcctg ggaagatact ttcccaggcg
    11821 gttctttgaa attgaatctc gtagtcgctg agtttttcat ctactgcctt tagaacttcg
    11881 atatcgaagc ctgtcagatt gcctttatct tcgtagtcaa atggtggcac gtcgccagct
    11941 gtagcaagga cgattgtctt ttgagcgcta gtctctttgg gtgtaacttg attcccacag
    12001 gcaaccaaaa atggtaggat agctagtaat aggctaaatt ttttcatact gtctccattc
    12061 aaatgtaaag tacttgctag tttatcagaa actttcctga taaaccaata tatatctttt
    12121 atgtctgtga tagaaaaact taatcatgaa aaatccctgc aagctctttc aaattatggt
    12181 agaatggaag cagtagaatt agaaaaggaa atcgattatg aaatttcttg aattaaataa
    12241 aaaacgtcat gcgaccaagc atttcactga taagcctgtt gatccaaaag atgtgcgtac
    12301 ggctatcgaa attgcaacct tggcgccaag cgcccacaac agccagcctt ggaaatttgt
    12361 agttgttcgt gagaaaaatg ctgaactggc aaagttggct tatggttcaa actttgagca
    12421 ggtatcatca gcacctgtaa ccattgccct atttacagat acagaccttg ccaaacgcgc
    12481 tcgtaagatt gcccgagttg gtggtgctaa caatttctca gaagaacaac ttcaatactt
    12541 catgaaaaat ttgccagctg aatttgcacg ctacaatgaa caacaagtca gcgactacct
    12601 agcactcaat gcaggtttgg ttgctatgaa cttggttctc gctcttacag accaaggaat
    12661 tggttccaat attatccttg gttttgacaa atcaaaagtc aatgaggttt tggaaattga
    12721 agaccgtttc cgcccagaac tcttgatcac agtgggttac acagacgaca aattggaacc
    12781 aagctaccgc ttgccagtag atgaaatcat cgaaaaaaga taggaagaag aaaaaatgat
    12841 agcaattgat tttacagcag aagtagaaaa acgcaaagaa gacctcttgg ctgacttgtt
    12901 tagccttttg gaaatcaact cagaacgtga tgatagcaag gctgatgccc aacatccatt
    12961 tggacctggt ccagtaaaag ccttggaaaa attccttgaa atcgcagacc gtgatggtta
    13021 tccaactaag aatgtcgata actacgcagg acattttgag tttggtgatg gagaagaagt
    13081 tctcggaatc tttgcccata tggacgtggt tccagctggt agcggttggg atacagatcc
    13141 ttacacacca actatcaaag atggtcgcct ttatgcgcgc ggggcttcgg acgataaggg
    13201 tcctacaaca gcttgttact atggtttgaa aatcatcaaa gaattgggtc ttccaacttc
    13261 taagaaagtt cgcttcatcg ttggaacaga cgaagaatca ggctgggcag acatggacta
    13321 ctactttgag cacgtaggac ttgccaaacc agatttcggt ttctcaccag atgctgaatt
    13381 tccaatcatc aatggtgaaa aaggaaatat cacggaatac ctccactttg caggagaaaa
    13441 tacaggtgct gcccgtcttc acagctttac aggtggttta cgtgaaaata tggtaccaga
    13501 atcagcaaca gcagtcgttt caggtgattt ggctgacttg caagctaaac tagatgcctt
    13561 tgttgcagaa cacaaactta gaggagaact ccaagaagaa gctggcaaat acaaggtgac
    13621 gatcattggt aaatcagccc acggtgctat gcctgcttca ggtgtcaatg gcgcaactta
    13681 ccttgccctc ttcctcagcc agtttggctt tgctggtcca gccaaagact accttgacat
    13741 cgcaggtaaa attctcttga acgatcatga gggtgaaaat ctcaagattg ctcatgtgga
    13801 tgaaaagatg ggtgctcttt ctatgaatgc cggagtcttc cgcttcgatg aaacaagtgc
    13861 tgataatacc attgccctta acatccgcta tccaaaagga acaagtccag aacaaatcaa
    13921 atcaatccta gaaaacttgc cagttgcttc tgtcagtcta tcagaacatg gtcatacccc
    13981 tcactatgtg ccaatggaag atccacttgt gcaaaccttg ttgaatgtct atgaaaaaca
    14041 aacaggccta caaggtcatg aacaagtcat cggtggtgga acctttggtc gcttgttaga
    14101 gcgcggagtt gcctatggtg ctatgttccc agactcaatt gataccatgc accaagccaa
    14161 tgaatttatt gccttggacg atctcttccg agcagcagca atttatgccg aagctattta
    14221 cgaattgatc aaataaaacg atagaagtct gagatcttat gcttggactt ctttttggag
    14281 ggaaagtaga tgtctcaaat cgaaagaatc aaacaggcta tcatggcgga ttcgcagaat
    14341 gccagctata cagagcgtgg cattgagcct ctctttgcag cgccaaaaac tgctcgcatc
    14401 aatatcatcg gtcaggctcc gggacttaaa actcaagaag caggccttta ctggaaagat
    14461 aaaagtggtg accgcttgcg ggactggcta ggtgtggatg aagatacctt ttacaattca
    14521 ggttattttg ctgttttgcc tatggatttc tactttccag gacatggcaa gtcgggtgat
    14581 cttccgcctc gtacaggttt tgcagaaaaa tggcatccgc agctcttaca ggaattgcct
    14641 gatattcagt taaccctctt gattgggcaa tatgcccaag cctactattt acaggagaaa
    14701 atcagtggga aggtaacgga gagggtgaaa cactataaag actatctgcc agcctatttt
    14761 ccgctagttc acccatcacc acgaaatcaa atctggatgg ccaaaaatcc ttggtttgag
    14821 gcagaagtag tgccagattt gaaaaaaaga attaaaacca ttttatagtc aatgaaaatc
    14881 aaagagcaaa ctaggaagct agtcgtaggc tgctcaaagt acagctttga agttgcagat
    14941 aaaactgacg aagtcggtaa catacgcacg gtaaggcgac gctgacgtgg tttgaagaga
    15001 ttttcgaaga gtattagaag aaaaagaatg aaagaaatag cctttgacgc attttaccag
    15061 ctttaccaaa acgaccagct ttctctagtg gatgtgagag aagtggatga gtttgcagct
    15121 cttcatttag aaggtgccca caacctaccg cttagtcaat tggctgatag ttatgattaa
    15181 ttggacaaag atcgcttgca ttatattatt tgcaaatctg gaatgagatc ggcgcgtgct
    15241 tgccaattcc tgttagaaca aggttataat gttatcaatg tccagggtgg catgttagcc
    15301 tttgaagaac tttaaaattt tgcatttctc ctacttggtg tggactgggt aggagagttt
    15361 tatttttaga taattcttat ttttaagaaa attgaaaaca tttaatattt gtctcgtgat
    15421 gcttttttca gactcctaat cgtggtatac taggtcagta ttttataaat atgaaggaga
    15481 tttttatggc taaaaaaggt accctaacag gtttgctcct gtttggaata ttttttggtg
    15541 cggggaactt gatttttccg ccttctctag gtgctctatc tggagaacat tttcttcctg
    15601 ccatagcagg ttttgtcttt tcaggcgttg gtatcgccgt cttgaccctt attattggaa
    15661 cgctaaatcc taaaggatat atctacgaga tttcaacgaa gatagcgcct tggtttgcga
    15721 ctctttacct ctcagttctt tacttgtcaa tcggtccatt ctttgctacc ccacgtactg
    15781 ctacaacagc ttacgaagta gggattagcc cccttttgtc ggatgcaaat aaaggacttg
    15841 gcttgattgt atttacggtt ctgtattttg cggcagccta tttgatttcg cttaatccat
    15901 caaaaatctt agaccgcatt ggacgtattt taacgccagt ctttgcaatt ttgattgtta
    15961 tcttggtcgt tctgggagct atcaaatatg gtggaacaag tcctcaagct gcttcagctg
    16021 cttatcaagc ttctgccttt ggtacaggtt tcctagaagg ttataatacc ttggacgccc
    16081 ttgcctcagt ggcctttagc gtaatcgcag ttcaaacctt gaaacaactt ggattttcaa
    16141 gtaagaaaga atacatttca actatttggg ttgttggtat cgttgttgcc cttgccttca
    16201 gcgctcttta catcggttta ggttttcttg gaaatcattt cccagtacca gctgaagcga
    16261 tgaagggtgg aacaccaggt gtttacatct tgtcacaagc cactcaagaa atctttggct
    16321 caacagctca actcttcctt gcagctatgg ttaccgtaac ctgcttcaca acgactgttg
    16381 gtttgattgt gtcaacagct gagttcttta atgagcgctt cccacaaatc agctacaagg
    16441 tttatgcgac agcctttacc ttgattggat ttgctattgc caatttgggt cttgatgcga
    16501 ttatcaagta ctcaattcca gtactggtta tcttgtaccc aatcacgatt gctatcgtta
    16561 tgattgtcat tgtcaacaaa tttgttgccc tttcaaaacc aggtatgcag ttgacaattg
    16621 ctgtggttac agttattgcc attgcaaacg tactaggaag ctcgtttaag gttgagtttc
    16681 ttgcaaatct tgttagcgtt cttccttttg ccaaggcatc tctcccatgg ttggtgccag
    16741 ccattgttgg aatcttgctc tcattggttc taccaaacaa gcaagaaagt gatgtttttg
    16801 aaatggaata atcactaata ctcttcgaaa atcaaattca aaccgcgtca acgtcgcctt
    16861 gtcgtactca agtacagcct gcggctagtt tcctagtttg ctctttgatt ttcattgagt
    16921 ataaaatcac tttttgtagt caagtctaca ggagtgattt tcttttttta tccgatgata
    16981 aatgtgttat aataggtagc aaaagaggtg aagaaatgaa tcaaacagta gaatatatca
    17041 aagaactgac agccattgcg tcgccaacag gctttactcg tgagattgcg gactatttag
    17101 tcaagactct agaaggtttt ggttaccagc cggttcgcac atccaagggc ggtgtcaatg
    17161 taactattaa aggtcaaaat gatgagcaac atcgctatgt gactgcccat gtagatacgc
    17221 ttggtgctat tgtccgtgct gtcaaaccag acggccgtct caaaatggac cgtatcggtg
    17281 gctttccttg gaacatgatt gaaggagaaa actgtaccat tcatgtggct agcacaggtg
    17341 aaaaagtatc aggaaccatc ctcatccacc aaacttcttg ccatgtctat aaggatgcag
    17401 gaactgcaga acgcacgcaa gacaatatgg aagtgcgttt ggacgccaaa gtaactagtg
    17461 aaaaagaaac tcgtgctctt ggcattgagg tcggtgattt tatcagtttt gacccacgaa
    17521 ctgtcgtgac agagacaggt tttatcaagt ctcgccattt ggatgacaag gtcagtgcgg
    17581 cgattttgct caatctcctt cgcatttata aggaagagaa gattgaattg cccgtaacaa
    17641 ctcattttgc tttttcagtc tttgaagaag tgggacacgg tgcaaactct aacattcctg
    17701 ctcaggtagt agaatatctg gctgtggata tgggagccat gggagatgac cagcaaacag
    17761 acgaatatac agtgtctatc tgtgtcaagg atgcttctgg accttatcac tatgacttcc
    17821 gtcaacattt ggtggctttg gcgaaagagc aagatattcc atttaagctg gatatctatc
    17881 cattttatgg ttcggacgct tcagcggcta tgtctgcagg ggcagaagtc aaacacgccc
    17941 ttctcggtgc tggtatagag tctagccatt cctatgagcg tacccatatt gactcggtga
    18001 tcgcaacaga acgaatggtc gatgcttatc ttaagagcac gttggtggac taatatgtgc
    18061 cttatttgtc agagaattga cctcatcaag aaggaagaaa atccttactt tgtcaaagag
    18121 ttggaaacag gctatcttgt ggttggagac caccagtatt ttgaaggcta tagtctcttt
    18181 ctagccaagg agcatgtcag cgaattgcac catttgaaaa aggagacaag actccgtttt
    18241 ctagaagaaa tgagtttagt ccaagaggca gttgccaagg cctttgctgc tgagaaaatg
    18301 aatatcgaac tgctaggaaa tggcgatgct catcttcatt ggcatctgtt tccacgacgg
    18361 acaggtgata tgaatggtca tggtctcaag ggtcgtggac cagtctggtg ggttcccttt
    18421 gaagaaatga cagcagaaac ctgccaagca aaaccggatg agattaaaag attagtcaaa
    18481 cgtttatcgt cagaagtaga taaactatta gaaataaagg agtagaaatg aagaaaagat
    18541 acctagtctt gacagctttg ctagccttga gtctagcagc ttgttcacaa gaaaaaacaa
    18601 aaaatgaaga tggagaaact aagacagaac agacagccaa agctgatgga acagtcggta
    18661 gtaagtctca aggagctgcc cagaagaaag cagaagtggt caataaaggt gattactaca
    18721 gcattcaagg gaaatacgat gaaatcatcg tagccaacaa acactatcca ttgtctaaag
    18781 actataatcc aggggaaaat ccaacagcca aggcagagtt ggtcaaactc atcaaagcca
    18841 tgcaagaggc aggtttcccc attagcgatc attacagtgg ttttagaagt tatgaaactc
    18901 agaccaaact ctatcaagat tatgttaatc aagatgggaa agaagctgcc gaccgttatt
    18961 ctgctcgacc tggttatagc gaacaccaga caggcttggc ctttgatgtg attgggactg
    19021 atggtgattt ggtaacagaa gaaaaagcag cccaatggct tttggaccat gcagctgatt
    19081 atggctttgt tgtccgttat ctcaaaggta aggaaaaaga aacaggctat atggctgagg
    19141 aatggcacct gcgttatgtc ggaaaagaag caaaagaaat tgctgagagt ggtctcagtt
    19201 tggaagaata ctatggcttt gaaggcggag actacgtcga ttaatactct tcgaaaatct
    19261 cttcaaacca cgtcagcgtc gccttaccta ctgactacgt cggttctatt cacaacctca
    19321 aaacagtgtt ttgagctgac ttcgtcagtt ttatctgcaa cctcaaagct gtactttgag
    19381 cagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtacaa aaaataaact
    19441 tttctcttgc aattccagat aaatagtgta taatggatgg gtatgtgaaa aacatacttg
    19501 tgggaggtaa aaatctctaa ttaccgccaa aaccacaaag gaggatttaa aaatggctaa
    19561 aaaagtcgaa aaacttgtaa aattgcaaat ccctgctggt aaagctacac cagctccacc
    19621 ggttggacct gctcttggtc aagctggtat caacatcatg ggattcacaa aagagttcaa
    19681 cgctcgtaca gctgaccaag ctggtatgat cattccagtt gttatctcag tttacgaaga
    19741 taaatcattt actttcgtta cgaaaacacc accagctgct gttcttttga aaaaagctgc
    19801 aggtgttgaa aaaggatcag gtacacctaa taaaactaaa gttgctacag ttactcgtgc
    19861 acaagtacaa gaaattgcag aaactaagat gccagatttg aacgcagcaa acgtagagtc
    19921 tgcaatgcgt atgatcgaag gtactgctcg ttctatggga ttcactgttg ttgactaatc
    19981 aataacacag tagaaaatct cacagcagtc tattagttgc ttttcatact aggcaagtga
    20041 ctgaggcttg tacttgggta cagcaaggga gcttaaggcc gtaggaagag aaaaatagta
    20101 gactgaaaac ccgcaagact tcatcatttc gagaagtgac gtgggagatg aaaatcgatt
    20161 gaaccactta caaggagaat agaaaatggc taaaaaaagc aaacaacttc gtgctgctct
    20221 tgagaaaatc gacagcacaa aagcatacag tgtagaagaa gctgtagcac ttgcaaaaga
    20281 aactaacttt gcaaaatttg atgcaactgt agaagttgct tacaacttga acatcgacgt
    20341 taaaaaagct gaccaacaaa tccgtggagc aatggtattg ccaaacggta ctggtaaaac
    20401 ttcacgtgtt cttgttttcg cacgtggtgc aaaagctgaa gaagcaaaag ctgctggtgc
    20461 agactttgtt ggtgaagatg accttgttgc taaaatcaac gacggttggt tggacttcga
    20521 cgtagttatc gctacacctg atatgatggc tcttgttgga cgtcttggac gtgtccttgg
    20581 accacgtaac ttgatgccaa accctaaaac tggtactgta acaatggatg ttgctaaagc
    20641 agttgaagag tctaaaggtg gtaaaatcac ttaccgtgct gaccgtgcag gtaacgttca
    20701 agcaatcatc ggtaaagtat catttgaagc tgaaaaattg gttgaaaact ttaaagcttt
    20761 caacgaaaca atccaaaaag caaaaccagc tacagctaaa ggaacttacg taacaaactt
    20821 gactatcaca actactcaag gtgttggtat caaagttgac gtaaactcac tttaatcagt
    20881 agttaaacta atgtaaaaaa gttgaagaca ctatgtctca actttttttg atgtacgacg
    20941 ggcatgtcgt atatctgagg tgtaagtcct cggtgggcac ctactaccgg tgaacccaat
    21001 agcgattccc aagtctgacg ctggaaataa gaatcgtcag aggaagggat agcgaaatcg
    21061 tggctctacg aacaggaacg tgataataag gcctatatag cggataaggt atctggctgg
    21121 ggcgaccact atcaattata gtaaaatgaa ataagagtag gacgaatcga tcagggcagc
    21181 caaatcgatt tctaacaatg ttttagaaga agagatgtac tattctagtt tcaatctact
    21241 atagtagctc agaagtcggt acttaaacgt gctatatcaa aaccagtcct tgaaaaacgt
    21301 gaacaggttt cgtgtttgga ttattacctt gaacgacatg cgttaaaagt tagttgaacc
    21361 gccgtatgcc gaacggcacg tacggtggtg tgagaggggc tagagattat cccctactcg
    21421 atttcgaaat ctagtggaat gaatctggaa tagtccatcg agctttctaa tactctttga
    21481 aaatctcttc aaaccacgtc aacgtcgcct tgccgtgcgt atggttactg actacgtcag
    21541 ttctatccac aacctcaaaa cagtgttttg agctgactac gtcagttcca tctacaacct
    21601 caaaacagtg ttttgagcaa cctgtggcta gtttcctagt ctgctctttg gttttcattg
    21661 agtataacac attgttagaa gttggtttaa attccctaat cagtttgttc acatttacct
    21721 tcgatatatt atatcccata gttaaggttg gtcatacaga tgattatagt catggagccg
    21781 taaaacttag tgtttcttta gttgacaaag atgccatgaa aaaaatatct gtaactgtaa
    21841 taggatattt tgaaataaat atagatgaaa atatcaccga tattctatac gtaaatggta
    21901 ctgctattct ttatccttat ttacgttcta ttgtttcaat agtttcggca attgatagca
    21961 gtgaagcaat gttgctacct accattaatg ttttagagtt actagataaa tctcaacctt
    22021 ttgaagaaga ataatttatt agctcactaa attgagggta aggaaaagta aaatcagtaa
    22081 gaaaaatgtc ttgcattata cagcaacctt ttgggaatga gtagatggat tgaataaaat
    22141 ttgattaaga gtggatgatt tatctgtaga ttattattgg acagttagtc ttgaagtagt
    22201 ctaagaatta ggttataatc agtagaagcc ttgctaataa tgaggaggtt agtttatgta
    22261 ta
//
LOCUS       DAVHEW010000024        22192 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVHEW010000024 DAVHEW010000000
VERSION     DAVHEW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.024"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(100..324)
                     /locus_tag="VVL53_000861"
     CDS             complement(100..324)
                     /locus_tag="VVL53_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU4183057.1"
                     /translation="MIDILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(329..508)
                     /gene="asp4"
                     /locus_tag="VVL53_000862"
     CDS             complement(329..508)
                     /gene="asp4"
                     /locus_tag="VVL53_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU4183058.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(510..1847)
                     /gene="gtfB"
                     /locus_tag="VVL53_000863"
     CDS             complement(510..1847)
                     /gene="gtfB"
                     /locus_tag="VVL53_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU4183059.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGTCFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINYSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGRQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1840..3351)
                     /gene="gtfA"
                     /locus_tag="VVL53_000864"
     CDS             complement(1840..3351)
                     /gene="gtfA"
                     /locus_tag="VVL53_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU4183060.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDEDKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3363..5735)
                     /gene="secA2"
                     /locus_tag="VVL53_000865"
     CDS             complement(3363..5735)
                     /gene="secA2"
                     /locus_tag="VVL53_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU4183061.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAILAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKEYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5728..6168)
                     /gene="asp3"
                     /locus_tag="VVL53_000866"
     CDS             complement(5728..6168)
                     /gene="asp3"
                     /locus_tag="VVL53_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU4183062.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGSTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6165..7700)
                     /gene="asp2"
                     /locus_tag="VVL53_000867"
     CDS             complement(6165..7700)
                     /gene="asp2"
                     /locus_tag="VVL53_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU4183063.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKSLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7684..9264)
                     /gene="asp1"
                     /locus_tag="VVL53_000868"
     CDS             complement(7684..9264)
                     /gene="asp1"
                     /locus_tag="VVL53_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU4183064.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHFHHDQLVLDRLPSTNPKLLSLFIGRNSQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRIQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9274..10491)
                     /gene="secY2"
                     /locus_tag="VVL53_000869"
     CDS             complement(9274..10491)
                     /gene="secY2"
                     /locus_tag="VVL53_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU4183065.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     KMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVS
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(10548..11225)
                     /locus_tag="VVL53_000870"
     CDS             complement(10548..11225)
                     /locus_tag="VVL53_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4183066.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11210..11452)
                     /locus_tag="VVL53_000871"
     CDS             complement(11210..11452)
                     /locus_tag="VVL53_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4183067.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(11452..12648)
                     /locus_tag="VVL53_000872"
     CDS             complement(11452..12648)
                     /locus_tag="VVL53_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU4183068.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGIDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFVFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12657..13877)
                     /locus_tag="VVL53_000873"
     CDS             complement(12657..13877)
                     /locus_tag="VVL53_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU4183069.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13908..16352)
                     /locus_tag="VVL53_000874"
     CDS             complement(13908..16352)
                     /locus_tag="VVL53_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU4183070.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IITTKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQYIEQLDYLAGQLPNVVFQIAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHELEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16362..17372)
                     /locus_tag="VVL53_000875"
     CDS             complement(16362..17372)
                     /locus_tag="VVL53_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4183071.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYLYMYNLSDILIVPSEQMKERLIEEGLTTKKILIQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWFQDTPLRVFSNKGEASSNARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(17401..17991)
                     /locus_tag="VVL53_000876"
     CDS             complement(17401..17991)
                     /locus_tag="VVL53_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU4183072.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(18006..18275)
                     /locus_tag="VVL53_000877"
     CDS             complement(18006..18275)
                     /locus_tag="VVL53_000877"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4183073.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGRTLLL"
     gene            complement(18315..19193)
                     /locus_tag="VVL53_000878"
     CDS             complement(18315..19193)
                     /locus_tag="VVL53_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU4183074.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLENLPNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENCHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVMIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKRRD"
     gene            complement(19190..21274)
                     /locus_tag="VVL53_000879"
     CDS             complement(19190..21274)
                     /locus_tag="VVL53_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4183075.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIISQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGISAARNTGLNNMSGNYVTFVDSDDWIEQDYVET
                     LYKKIVEYQADIAVGNYYSFNESEGMFYFHISGDSYYEKVYDNVSIFENLYETQEMKS
                     FALISAWGKLYKARLFEQLRFDIGKLGEYGYLNQKVYLLSEKVIYLNKGLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     ATYKEFEMKQRLLNQLSRQEESEKKAIVLAANYGYVDQVLTTIRSICYHNRSLRFYLI
                     HSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKTDISYTVFLRYFIADFVQEDK
                     ALYLDCDLLVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDLTNEWHNKVEQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYAVDQV
                     QAMIEKLREISK"
     gene            complement(21335..>22192)
                     /locus_tag="VVL53_000880"
     CDS             complement(21335..>22192)
                     /locus_tag="VVL53_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4183076.1"
                     /translation="SSSASTSASESASTSASASASTSASASASTSASASASTSASASA
                     STSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAST
                     SASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSA
                     SESASTSASASASTSASASASTSASASASTSASASASTSASASVSTSASESASTSASA
                     SASTSVSNSANHSNSQVGNASGSTGKSQKELPNTGTESSIGSVLLGVLAAVTGIGLVA
                     KRRKRDEEE"
BASE COUNT         7030 a         4750 c         3540 g         6872 t
ORIGIN      
        1 gcctgcggct agcttcctag tttgctcttt gattttcatt gagtataaaa aagaaaactt
       61 cagaccaatc cacaaaaggt gaatatctga agttttcttt tatttataag taatcaccat
      121 aaaggttaat agtagaacaa ataaagccaa actcaccaat aaagtgagca ccttgaccaa
      181 ggtgttgctc tgccagtagc ttggtttacc aatattacta gtggcatcca tggaaaatag
      241 ttgattttga cggggttgta tagttaccaa tacaatcaaa gcaagagata ggataatagc
      301 taaaacaatt agtatgtcaa tcatagatct acctcaaaat tcccagcaaa gtaaagagca
      361 aaccaatcag aatcatcaaa cccagtaaaa gtgaaaaagt cttactaatc ttttcccctc
      421 gggtttcttt ttccttcttg atgggttttt gtttcaactc ttccatgcgg ccttcaacgt
      481 ctttgtaaaa taaatcttcc tctgacatgt tagcctccta aaacagtttg catggtttcc
      541 tgatatctca ccaagtcaac ataatttgca tgttggcctt gtctgccaag tgcctgacgc
      601 atttgatcaa catctgaaag ggccaattta atggtctcaa ccaaagcagc aacttcacta
      661 ctttcaaata gatggtctgg agcgatataa agtctattgt gaaccgtttg attaaatcca
      721 agaatcaaga gattgtgctc aaaggcctga cgaactgcct gcaataactc attgctatag
      781 ttgatatcca agtaaatatc cgacagttga tacaactcct gaatcttctg tggactagca
      841 ttctggtaaa gaaccacatt aggatagcga agcatgtcta agagcttaga agacatctct
      901 gtcaccgctg cgatacggaa ggtaacatca ggcaaggctt ctacgattgc ttctacttgc
      961 tcaatctgat ctgaattggt caagattaag gcgtctcgtc ttaggaaatt atcacgtttg
     1021 aactggtaat gataacccaa gtgcacaaac tgatcatggt atttctcgtc agttaactct
     1081 aaagcgcgct cataagtcgc cttatttgga ataatgatct tcttagtacg cacattatca
     1141 ctttccaaaa tcaactgcat attacctgga atggcatcat aaagaggttc ctgccatacc
     1201 aagacatccg agccagattt atctggataa tgaaaggaaa ccaagaaagg agtcgctaga
     1261 gtattaaaga taagctgact ggtgtctatt tccaaatctt gcaaaaagaa ggtgataaat
     1321 tcaactttat ttgcaaagta acgcatggac tgaccaggca aagtcaataa gatatcaccc
     1381 gtcacatggt tttccagtaa aacttgttga ccattgacat cttggtaaac tgtcataatc
     1441 ggctcgctat ctgcgctata agtggtttta gcaaaacaag ttccgaaacg attatagtgg
     1501 tcaacctgac gtactcgacc ttctaggtct ttccagtcta cctgtttaac caagcgagcc
     1561 tgcattccat cagcataatg aatgacagcc ctctcctgcg tcacatcttc aatacaagca
     1621 gactgattat ctcctaaaat ttcccaaaaa tctgaaacgg gaacttggtt aaaatagaga
     1681 ggttttccat cctcgtaacc tagataatag gtaaaaggag aaagcagacc atcaggcaga
     1741 aaaccatctg catcgatgac cactccaagt tgagaaagac cagtagcgac tagactttca
     1801 tgtaaatctc gactttcctg actgtaactg tcataaagtt caatcatgga gcacctcctc
     1861 tactgttttc ttccactttt ctaaaatttc tttggtcaag aagccttctg caatttggta
     1921 agaataggca cgcatagctt ccaaacgatt ttcttgatac aattgacaaa tcttagcggc
     1981 ataagcttgc ttgatttggt cttctacatg gtcagatgaa cttggaatca aataaccatt
     2041 ttgcccatcc tctataaagg tctgattacc ataaggcaca tcaaaaccaa ttagaggtag
     2101 acctgaacca atagcttcca tcaaggtcag accaaatcct tcgctggtag aagccgttaa
     2161 gtagacctca tactggctat aaatctgcga aagttccgca tgccccttga gttggatata
     2221 gtcctctgcc tgatgatttg caataatttc tctaagcaga gaatcttctc caccactacc
     2281 atagatatca aaggttagtt ccggtaactc cttatgagct tcaatcacag ctttcacaag
     2341 ccaatcaatg tgtttttctt tggcaagacg tgaagccgta atcaatgaaa atggtttgcg
     2401 cccttgactt gaatctgtca aggaatcaat actgcctaca ggaatggtaa caatctttgg
     2461 ttgatgctga gtatatttgg caaattgctc ttgtagaact tcattttgtc tatcagtaga
     2521 cacgataaag aagtcaacct tatctgcatt ggtaaactga tagtcataat agttattcca
     2581 aaggatatag tcctcatttg tagcattttc actataatgc tccgcatgaa caactaccgc
     2641 tagatgtgct gtctgtgctt cctcaaacac aacctgtcca atacctgtct ccctatccag
     2701 aataaccaaa tcagacttgt tcaaattcaa agatttcata aaggcacgca caaaagcttg
     2761 ctttccatag aaaatcttat ccttgaaatg ataaacttct tccttccctt gattcatcaa
     2821 gatatcatag actggagtcc cgtcttcatt gtaaaaagtt cgttggtata agactgcaac
     2881 attgtccttt ggagcaaaat actcgctaca ataacgcgta taagaaaagt aatccttccg
     2941 aatcaggttt cccttaaaaa catactcggc atgttgaacc aagtccttgt cctcatccac
     3001 caaataacag gttacaaact tatcttggtc aaaaaagaag acacgtaaaa ccttgccatt
     3061 tttttctctg tgactttctt caccaccaaa gtaagccaag acatcatcca ctgtcacgct
     3121 agtaggcgca attttgatgt ctgtgaaatg attataaagc cagataacct gattatcatc
     3181 aaaaccaata ttggctgtta agtgctgaat attatcggct aaaatcatat ctgtaaagat
     3241 aaacttagag gacagattta atttccgaaa aacaccagca cgataggctt gagcgtattc
     3301 aacaccgcta ctagcccaac caattcctaa atttatattg taaattgtca tattttctcc
     3361 tcttatggaa aatgagtcac gatttcacct tttggagtga cctcaaccag ccccatcagg
     3421 agattacgca ccatatccgc acggatttgt tctttcatag cttcaaagcc cgcgtaggct
     3481 tcttgatagt actctacgat tggatttttc tgactagcgg attgaccacc gatagccatg
     3541 gatagctgtt gtagatagtc tacctgctct acccagttgt catcaatggc tttaagcagt
     3601 gaaagtcgta aaaactgttc atataagtca tgttgattaa gtaattcttt cttttcagaa
     3661 agttctttat caatcacctg cttcataaag ctacgaactg cagttttgtc agttacatct
     3721 atataatctg gaacctcttt aacatgaaaa ctaatattgg tcacaataaa gtgaaacagt
     3781 aattcacgac tagcatagtg atcagccgct acctcttctg tatatctctc aatgatatcc
     3841 acaacaacat cctctaagtc acgagaacca tctattagac gattacgctc cttgtaaatc
     3901 atatcccgtt gtatattcat actttcagca tattctagag tctgacgacg tgccgaacgg
     3961 ccagcactat cactagcatg ttgagccttt tcgactagtt tccggtattt acgacctttc
     4021 aatacttccg gttgagtcat atcttgaacc tgatagtctt tgtacttttt atgcacccaa
     4081 gatggaccaa atttcttgat aacatcatcc tctaaggata caaaaaattt actcatacca
     4141 ggatctccct gacgaccaga acggccacga atttgtaggt cgatccgctg actttccatc
     4201 cgctcagtcc caataacaat caagccccca agctctgcga ctcctttacc aagcttgata
     4261 tccgtaccac gtcctgccat agaggtagcc actgtcacag cccccatctg acctgactcg
     4321 gagataatct gagcctcacg cgccgcatta ttagcattta ggacattatg ggcaatccct
     4381 tcacgaaaca agagagacga atagagttga gacatttcaa ctgagcctac aaaaacgagt
     4441 aaaggatttc ccttagcatg gtattccttg atgtactcca aggatgcata cactttttca
     4501 ggtaaagtga tatatagatt atctggatag tcaatccgtt gtctcggacg attggttgga
     4561 atgcgtacta cagacatatt gtaagtttca ataaactctt tttccgcgac cttacctgtc
     4621 cctgtcatac cagatatctt attaaacatc ttaaaaagac tctgataggt gatcgaggcc
     4681 atagcccgcg tctcaggaga taatttgaca tgttccttgg cttcaatagc ctgatggaga
     4741 cctccttgaa gtttagtcat ttccattaga cgccctgttc ccttatcaac cagtaccatc
     4801 tcatttccac gaatgatata gtccttatct ttagtaaaga gcttatgagc tagaatcgca
     4861 taaaccaaat gacgagcaaa agacgcatgc tcttccttgt ataaattatc aatccctagg
     4921 aaattctcag cagacttggc ccccttagta gtgagccaaa cctcctcttt ctcctcttta
     4981 aagatataat cctctccttc gaccaaggtt gttacaagtg tatcaatgat cgcatagtaa
     5041 ttagactgaa cacgaggaga acccgcaata atcagaggag tttgtgcact atcaagcaag
     5101 atatcatcaa tttcatcaat aatcacatag ttaaacggtc gtaaaaactt accttcttca
     5161 ttcgaggcta ggttatcatt tagataatca aaacctaaat tactattggt tgtgtagatg
     5221 atatccgaag catagataag cttcttttct tcagctttca tttccttctt tggatcttcc
     5281 gtaaatggta caccaatggt caatcctaga aaacgataaa cttggcccat ttcctcggca
     5341 tcacgctttg ataaatactc attaggagtc acaaccatca ctccttctcc tgaaaaagcg
     5401 ttcaaataga caggcatggt agctgtcaag gtcttacctt cccccgtatt catctcagca
     5461 acatttccat agtgcatgac aatagctccc atgacttgaa catcataggg gaacatccct
     5521 aaaatccgct tatctgcttc acgcaccaca gcaaaagctt caaccaaaat atcgtctaga
     5581 ctttctccct cggaaagacg ctgacgaaac tctactgttt tagctactaa ttcttgatcc
     5641 gaaagagagg acatcttgcc tttcagggca ttaatctgct ttaaaatctt tttcactttc
     5701 ctaagctgga aatcttgacc taaacgtcta aacacttctg atctccttga tagaaaaatg
     5761 ataaaaagtt aaggactcaa atccagcact taaaagagaa acttgataag tataactatc
     5821 ttctggataa gtaaatatga aatctttcgc tttttctacc tttttttcta aaatatcacc
     5881 atagcggtca aaaaaagtta tcttggtata aactccattt tcaggctgac aatcaaaaac
     5941 cagtgatagt tgatactcct gctttctttt taataaggga agctgaggtt gggttcgtgc
     6001 agcctggtaa ttgacactag aaaaccaagt ctttagaatc tcaccagatg gcaatagagg
     6061 attatacaga cgaacacttt tgtcagggta ataagataca gttgatccat acatataagt
     6121 cccaccaact tctccccaat gaatagactg tctctgtgta ataatcatct tttctccctt
     6181 ccaaaaccag tctctaagac cattctataa aagtgcaaaa accaagaaat attggtatct
     6241 gtatcatcat tgtgtcgtcc agcagttcct ttagataaaa tcttcgcacc tgtattacac
     6301 agataagaca ctaattgttc ataggcttgt ggatccattt cttcatcttt catataggat
     6361 aaaccaaagg ttgtctttga aaaatttgct tgtttaaaaa ttttccaaaa acgctgatcc
     6421 aactccgtca tatcttgaga acttgtccca cctgtatgat gaattaaaca atcaaaagct
     6481 aagttagaga cacccggtgc gtccaaacgt ccacgactag ctatagttcc taaattagct
     6541 aagggcttac caactatgat ggcatggggt tcaaaagaag caccataata gagagccgga
     6601 aacgttccca tagaaagacc tgataaaatc aaatccttat gatcaaagcc taaataatca
     6661 agatagtgag tgatcgtatc ctttactttt ccctctagct catccgttcc gagataaaaa
     6721 gctcccccct ctaaacgtgg atcagaaaat aaaatgaagg gacatccaag agttttcatc
     6781 ataaagtaac cctcgaatcc ttctgcaggt cgataacctg caaaatagac agtcaaaggc
     6841 ggtttaaaat cacctggatg gaagaaatag tttatttcat cacgcttgga atcatgtagg
     6901 atatttccac caagtacaaa cttaccaaat tgttttcgac tccatcgttg gtgaagatta
     6961 cctagtttga tagttccacg acctcttgct tcaacagata tcgagagaaa gtagtctccg
     7021 tctcgactat ccataaaaag tgcttgctcc aagtcttttt cagatactat tacttcttca
     7081 aagaaatcat caacagaccc actccacatt ttacggatga ccaaacgaaa gtcacaattg
     7141 ccttccttct catattcaag ccacaactca atagggagtg ttttttgcac cataatatta
     7201 taagcccaat aggcaagctg ggcaaaatct tgcccaaact caccttccaa actgacataa
     7261 tccaatcctt gataagaaat agctcctgta aaatttggat tgacttgtat cgtcggagga
     7321 aagagtttat caccataccc accgccaaaa agagatttac ttaaatcatt gattaattgt
     7381 tggggactta aaaaatcaat tgcttgtgca catcgttttt ttagaaaatc cacaacctct
     7441 ctatcattta tttgtagatt ctggttatag aacaaagtat aaggttcaaa atattttaca
     7501 taggaaaata agtctctgga atactgacca tcttctatta aaactgcatg aaaacggtta
     7561 atatcttctt tttcgattat ttcacgaagg gattttgatg agtttgggta aaaataatac
     7621 caactcatat tttcaggtat gtcatagtga ttttcccaat tggcaagtcc tatctgtaaa
     7681 atattaagtt cctttgacat ttttaacctc ctctatccat ttctctaatt ttccaagaaa
     7741 ttgttgtcct gtgtgctcct taatcttatc aattgaatat atcaaggact cattccactc
     7801 cttcaaccta tctgtgtaat aatgtgcagc cttagaaaat tctgtaacat ctgctaacaa
     7861 ataaccattt tttaaatgtt caacatagac ggtttcaact aggttgattt gaggaattcc
     7921 tgcactaatc ccagcaatct gtgtgtagag atgaggatgt ctatttaaat ccacaatcaa
     7981 acggacaaat tctagtgttt ttatcaaatc taactcatca ttcaagttga caaactgtat
     8041 acgtaagtcc tggtgttgat tttcttccag gggattttct gcatcaccat aatcaatcgc
     8101 ctttcccaga ttttcggatt gaatgttttc ttggatgaaa gactcaacaa tcccttcaac
     8161 ctcattcatt tgctcctgac tagcagcaaa ggctccaaaa atcacctcag tatccttatt
     8221 ttcagcgaca aaggacaaga cttgaagcag agcttgatta tcaatcccct gctcaaaatc
     8281 cagttgaaaa taaatgatgg attctttctt agtttggctt cgtcccaatc gtaatcgggt
     8341 gtcaaaagat gataaatgat aacattgatg cattcgttca ggatacaact cctgcaacaa
     8401 tcgtaaacta tcctctctat ccaccaaaat caaatccgat ttttcaaaag ttacatctaa
     8461 atccctaaag gtatcttgag aattacgtcc aatgaacaga ctcaacagtt taggattagt
     8521 actaggtaaa cgatctagta ccaactggtc atgatgaaaa tgagaaggaa gcataaaaat
     8581 atcctgatcc ttcacatacg tttgcagata attctcaaaa aattctgcca ccagatctcc
     8641 catattttga taggtaagca ttttaaagcg ataacccaaa attggattga cttctacctg
     8701 tcctccttct tttaaacgct ctctaaattg ccaaactccc ttaggattca aatattcttg
     8761 ataggctgct tgcccatctt caaaaaaaat aatactcgat ataaaccctc gatcatccat
     8821 gatatggtgc atatgtattt gaccattcgg tccaaaatac tgtatatcac tgatgaagcc
     8881 ttcaactcca tgttcaacct tagcaaattt cttcccattt ttttgaacga taatcgtaaa
     8941 gggactataa ataaattcac agtcatcatc ccactcaata tctctaatgc ttaaaacttg
     9001 ggtatggaga ttatgaaaat cttgcataac atcaaaaacg gaataagtat ccgtttctaa
     9061 cacaccatgt ctatataaaa aataacgtag atgaggctgg taagctaata ctaatagacg
     9121 agaatctata tcttgctctt gaaagagccg aatctggtga aaggtatcat caaactctag
     9181 acgaaaatga gaaaaatacc atggagtgat atctgcatgc catgttcttt ctgacccata
     9241 ccaagctgga ataaaataat acataaaagt ctcctaaatc aaaggtctat aggtctcatt
     9301 tagccttaaa gccttgacct cgtctctaat cgtaaaaatc atgcccccga acatcataaa
     9361 taaacctgga atcattgcca atcgtaataa cttttcatca aacaaaacaa aaagcatggg
     9421 accacctgcc ataaccacat taaagagacc acctatgact gagaaacgaa ggaccaatcg
     9481 attaataaat cgactagtat ccgcacctgg ataaattcca taaatgtatt ctccagattt
     9541 tttcatacgg tctgcaatct cttctccatt catcgtaaca aaagcaaaaa tgatactaaa
     9601 taaaaataag accgaaatat aaacatagac ccacaaaggc ttcccaacca taaattcctt
     9661 tgataaagcc gctagccctg aatgattagg gaaaataaat cccaacaaga tgaacaaata
     9721 agctggtaca ctaagaaaac tcatcacata catataaggc atcccacctg caggattcaa
     9781 catgatttcg agataagaat agcgtttaaa tcgattgtgt aagccaattt tattaacaga
     9841 aaccaaatag cgagctcgat acataagggc aagcaagtaa gaaaatataa tggtcataaa
     9901 aacaagcaag acaacaatcc ctgtagggat ataaaccagt ttaaaagttt caataacatc
     9961 ctgagggata tttaaaacaa tgctggatag gaggattaca atagaacccc caattcccat
    10021 gctcgcattt aaatctgaca accaaacaag aaaaaacgtt cctgctatga gcaacaaggt
    10081 attcattaga gcaaccaaaa ttaccgagta ggaggattgt actggcaagc tcaagctaac
    10141 tgccaaggat tgaatcacag caattagcaa ggtcagatac atttttcgac gatcttgaat
    10201 ttcaatcgct gtagaactta gccccaattt tttagaatat gaaaacatct gccacaaaat
    10261 catagctgac atccaagggg ataatccaac agaaaaaatt gacaaacttc ttagatttcc
    10321 acctgttagg gcggctgaga aggctagata ggctgttgaa cctcctaaaa aatctttagt
    10381 atttaggtca acaaaaggga gagtaatacg acttcccaat acatagataa acagtagaaa
    10441 taaggtaaat agtccttttt ttactgctat tgacgaataa atcttagtca taaaacactc
    10501 ccctcacctt cattgtttgt gatatggtaa ataacaattc ttttatttca acttttacca
    10561 tttctttgtt ctgcatcaaa aaacaacttt aatctgaaac aaatatcttt atattcctca
    10621 gtatctgtta attgatggta ttctaaagtt tgcttataat cttttaaaag attgacaaat
    10681 cgaatacgca gaacggagac atccaaacca gccaagacac aatccgatat tttcttagaa
    10741 aacacttcaa caatatctct tgcccaggaa agatcaaatt ccgttctcat gatgcttcct
    10801 gaacgtcttc tatacagata gagattatcg tttataaaga cgattttaga ggctaaaaga
    10861 taaaagcgat gcatagttgc ttcatcatca aagcggcgac catttgaaaa gtgaacttca
    10921 ttgaataatt cttttttaat caacttaaat gtcggcaata taaaggccga gctattgaat
    10981 ttccaatctc ctgcttgacg gttcataatt tcttgtatag ctaattcttc gacgcaaaaa
    11041 tcttgccctg ttacataaaa ataaaaattc ccgtcacttt cgtcataatt atagtaattc
    11101 cctataacaa catctgcatc cttttcttga acttgctgat aaagagtttc aatcatattt
    11161 tcttttacga tatcatctga atcaacaatc gtgatgtact ctcccttagc tacactaatc
    11221 cccatatttt tagcttgtgc tgccccccct tattttcttg atgcaatact cgcacacgat
    11281 tatcttgcat cataaaagca tcacaaattt ccccagaatt atccgtagaa ccgtcatcaa
    11341 ctaaaataat ttctatattt tgataggttt gcttcagaat atgctcgaca cactccacta
    11401 aatattttcc cgtattgtat atcggtacca caacactaat taattcactc attactctat
    11461 ctctatattc ttcattctat ccactaaatc atctggtctc tccacagaaa aaataccgtc
    11521 atagagtcca tcatccatac ttttacgtgt gatatcaaaa acgaagattt tcttgccact
    11581 ttcttgtgcc cttgtaacta cattaaaaac ctctccacct gtattaatat ctaaataaac
    11641 tatagaatcg tccaatagcc aatcaattct actatgtaat acattttgat atactgttac
    11701 attcgtatac tgtgatagag aggtcagctc ctcagaacaa tcacacggtg cagccaaatg
    11761 aaaatgccaa tcaggtaacc gttgtactaa atactctaaa tgttttatat ctgcactcca
    11821 tgtcacaagc atcacttgtt ttttagactg attgcttctt tcaaaataat ttaaatcgga
    11881 acgttgaaaa gcaatctctg accaatccaa atctctataa acccaccata attcacgtag
    11941 tctagatata ctatatgtat tccaaggttt atcatgacta gcatagtgaa caattgtagg
    12001 tggattgtca tctagacgtt cacattcttg agcaaggtga taaatatcaa tacccaccat
    12061 ataattatat gttttatcta aggctagcca attatcctca aaataaatat ttaaaatact
    12121 ctgatcccca agatgaacaa cttgattctg ctcggccgct aattccaata aactattgac
    12181 aatagaatgt tctttccact ttgcaacatc cattagtaac ataccagtat taaatccaga
    12241 ttttcgtcct tcataggcat agacatcatc aacagcacca attgaatatc ctttgagatc
    12301 tatctcaaac aaagtagcta gttccccagt tacaatgata tcggaatcca gatacaatac
    12361 cctatcagat tcaaccactt ctgtcgcaaa aaatctaaag taagaagcat aatttatatg
    12421 aggtcctgtt ttatagcttt caaaaagttc tttatcaatg tgtacactgc gaattgtaga
    12481 attcacaact tccatttttc tccccaataa tttaaaccat tcaggagcta tatcactgtt
    12541 gagaatataa aaatcaacat ctctattgtg atacaataca gactttatag tcgtctctaa
    12601 aggaataaga taggcattat ctgccgctaa tactattgat tttctcatac tgttctctac
    12661 ctcatgtaag atctgattgc tgcaaccatt tttttcggaa tggagcttgg atagatacct
    12721 tcatatgaaa tctcagaaag attttcagag caagtattgt cgaaagctat cattggtttt
    12781 ttgtacttaa gcacaaactc atatgcatct tctaattttc gatcatgatt caaatccaaa
    12841 tacaaatctg aagctattac tatttgttcc aataacattg gtaaggaatt tggatatacg
    12901 gtaacattag gaaaacgcgt taaagctagt agttcatttg ccatatttgt ataagcaaca
    12961 atatgaaaat gaacttctgg caattgctct gccaaataat cgattttctc gacacaccaa
    13021 gaattcgtta aattaacaca ttgcaactta aatatttgtg atttactagg gtacttcaca
    13081 ctctttgaaa accattcatt taaaataaca gaccaatcca tcaaagagta ttcccaccaa
    13141 acttctctta gacgtccaac agaaaattga ttccaaggct tatcctgaga aatatagtgt
    13201 aaaattagtg gcagtggttc gagcggaata tcaaaaatga attgatgttt aaaggctgcc
    13261 gccccataat catatcctat ttgaaaatta tattgatctt caagggaact atattgatct
    13321 ttaaacaaca tattcaaaat tgactggtct ccttcttcca cattctcatg ttctttttct
    13381 gttaagtcaa tcaatttttg tcgaatagtt tcagatcccc attttttgtt gttaatcaag
    13441 agaacaccag cattgaagcc gactcctgct ccaaagcaag aacgagctgc tgccaaataa
    13501 ttttcaccta agtctaattc aaacaaatcg gtcaaatcac cagtcacaat caaatcacta
    13561 tctagataga gaactttatc ttctgttaca aaatctggaa taaaatagcg tgcaaatgtc
    13621 atatgattga tatgaggtaa tttattagac caattcattt gaaactgtga gccaattaac
    13681 ttgcagtcaa tcaagtcgcc ccccatctct tgtaaatata ttcttatttg actaaaccat
    13741 tcctgaggaa tgtcctgatt tagcagataa attttcaaat gactattgtg tctacagagt
    13801 gacttcatcg ccgtttcgat ttgtcgaata taagcgtaat cacctgcaaa tactacagcg
    13861 cgtttggtat ttctcatttt aaatccttat catttatttt aaatagtcta gtttaatatt
    13921 cgtgcaacaa tctgactgtt ataggtttca tcatcaataa attcaatatc ttggtcgaaa
    13981 ttatgttctg cagtatgttt atgagaaaat ttaaccttag ttttagctcc cattttcatc
    14041 cattcatact ctgagtcaat atggcctaca tccaaaactt gatagcccat ctggcataga
    14101 ttataactca gaacttttgc tgtaggtcca agcatacata aaatcaagcg accaccagca
    14161 tacttttcaa tttcttgttc aagttcatga actctagaaa aggcactatg agaaggacag
    14221 ataattcgct taatagaatt tgcctcatcg aataaatcat ttccgacacc tgaacgagaa
    14281 gtcgcacctt cgactatcag taagtcacgg ttttcccaaa tgcttttcaa tttttcaaat
    14341 tgagctttag cttgactctt gtcttcaaaa tcgatataag ggcgagatac aaaggttgag
    14401 ccataccatg aatcactgca taactctctg taaaaatcca tataatgatc catgtgatct
    14461 ttccaaaatg gaatcgccca cgatgtaaac ctaaaacgat ctgtaaaagc atcaggaagg
    14521 cacactacta aatcttctcg actttcttgg ccgataatgt ccctcatgat tgaaaccaac
    14581 tcttcatcat aatcctggta gggaattgaa tgccctgcaa gcatattgat ttccccatct
    14641 ccaaaacgaa ctaaagaaga gttgtgttca ataatataat ccaaggtttc atctatccct
    14701 ttcacctgga ttatatcaag cggtttttct agcatatcct tagttatcaa tttttgcaaa
    14761 tcagccaaca ttcgttcagg atgttcactt gaatacaacc tttggccatt attcccattt
    14821 tgagatttat aaaaggctag tataggttta gatagatgag ccatttctgc aacgatatcg
    14881 gatgttgaag tcagtaggtt gatatctaaa taagcatcca tcttctcctt caactcgtct
    14941 agcttatctc tagaaattgc cggatagagc tgaatattag gataacgatt agacaaatca
    15001 tataattttg gccccatagc tgtccaagca gcaatttgaa aaacaacatt tggcaactgt
    15061 ccagccaagt aatccaattg ttcgatatac tgagaagccg taaacgttaa acaagttaaa
    15121 cttggaagtt gtcgaaaaca ggtaagatgt ccgtttttct taaaatcttt aatcgtatca
    15181 atcataaagg aaactttccc attttcgaaa acgatttgtc ctaactctcc gtgctgagtc
    15241 gattgaaaag ctagtagcgt tttttcttgt tcttgcaaag attttaaaaa gttctcatct
    15301 gcactaccat gattgatatc caaatataga tttgttgact ttttcagctt atctaagacc
    15361 ggtggaacaa tttgtggatg caatctcaca ttattatata cagctaattt ttttaattta
    15421 tctcccatat ccgtccaagc tgcgatatga aataccacct caggtagagc tgtaactagc
    15481 tcttctattc cttctaaatc ttgggaattc gttaaggtta agcaagaaaa ttccttatct
    15541 agaggcgata ttagttcaaa ttctcccata tggtgttgta aaatctgact ccactccaaa
    15601 tcatggaatt cccaccataa atcacgataa cgattggctg tcaaggtagt ccagggtttt
    15661 ctgtaggtcg taaaatgaat caccactggt ttatcattaa aagccagatg ttcctgccaa
    15721 ttgttataca aagccacaat atcatgccct acttgtaaat tataagctcg acctagttct
    15781 aaccaatcat cttgcaagac ctgattgaaa attgtttgat caccattaaa atgctcaaag
    15841 cggccttctt caacttcctt tgttgttatt atagactgtt caattagtcg ttcttttaat
    15901 ttctcttgac gccatttttt attgttgatt aatagaacac ctgtattaaa tgtaattcca
    15961 tctgtatctt taactgctgc gagtgatttt tcttctaaac aaatactaaa taatttctct
    16021 aaagaagtat ttacaatcaa atcactgtct aaatataaaa ccttatcttc ttggatataa
    16081 tctgcaataa aatatctagc ataggtaatg ctactaatgt ggtcttgctt ttcccaatct
    16141 tgaaacacag tttgttcagg tagcttcaca tcgataatct cactacctaa catgcgagct
    16201 atttttcgtg gctttcgaaa ccaatctggc atgatatctt gatttaaaat ataaatctta
    16261 acatctcgat tgtggtataa aatagatttt atggttgttt ctaactgcct ggtgtaattg
    16321 cgatcccctg ctagtacaat tgttttgttc atttcattcc cttaatcaat tcccaagtga
    16381 tagattgcat ctaccaataa ctttttagtg aaatacccct cttttaacaa atagctaaag
    16441 gtcttgatac gattcgtcat ttcttgatat tcttgtagat tcattttatc aactatctca
    16501 tgaacctctt ccagactatc cgccataaag cccaagcctt gatctactat aaatttagca
    16561 gttgacaagc tacttggtac aatgactgga atgcccgctg tcaaataggt actaacctta
    16621 tgagatatat tcaaggtata gtattggtta ctttctccct cattttgatg ggttccccag
    16681 acaagaccaa agccaccttt tgataattct agcaacagtt cctcatcttt tttccatcct
    16741 tcaatgctaa gatttctagc attggaacta gcttctcctt tatttgaaaa caccctcaaa
    16801 ggcgtatctt gaaaccaatt ttgtaagtct ggaaaacgct ctaaacttcc agcaaaaaaa
    16861 agttcttttt taaaagcagg ggtgtataag gataaatcat gaggatgatc ccacatccct
    16921 tgaatgagaa tcttctttgt tgtcaagcct tcttctatca ggcgttcttt catctgttca
    16981 gatggaacaa tcaaaatatc cgatagatta tacatataca gatagtcttt catgagatag
    17041 taattactat caaacatgag aggaacaaca tcatggataa aacaaataat tttcacctgc
    17101 atatccttca gcttatcaaa caagagacga tcaaactcaa agccattcca agttggagac
    17161 tgaaaaacta ggatatctcc aatagagata ctggccataa tgccatccag acgcttattc
    17221 atttcagaag gactatctga agcaatgttg taaaaataaa taccaacctc tctaaatccc
    17281 agttgactag ctatcttttg aacagcattt tgagctaaga taaccgtact atcaccagcc
    17341 atgccgtata aatttgttaa atgtagtttc atattacgct atctcatttt ttattactat
    17401 ctacggaagt aaagacaatt tttccatcat tcttctgtaa atctctgtga actgcatatt
    17461 ttgttcttct aattttgcta tcactctttg taatctattt ttataaatca aaatatgatt
    17521 ggtcaagtca tagtctgaac ttgctaaaat agctatcttt tcttccctag ccccaatttc
    17581 atcatacata cgcttttcac tccatgtatt cgataaagta tcagataaac caacacggta
    17641 ccagtaaaca caacgattca aatagactat cctcgaagct cttagaagta ccttccatgt
    17701 ccagtaagta tcctctgcaa tttttcctat aggaaatggt aaatcttgta gtaactctct
    17761 cttgaataac ttcaagacag ctacagtcca gttcccattt ctgacttctt cgacaccttc
    17821 cctatccata attgctttac cttctatcac ttctagagaa tcatctggat ccgtaacata
    17881 agtcatatac acatagcgtg tttcatcata agaattataa cgcccgatac taatatctgc
    17941 gttttccttt ttcaaagcac catataatcg gtctaaagca tcatgttcca accaatcatc
    18001 agagtctaca aaagtaatgt acgccccccc gaacattcaa tacctaggtt acgagctgat
    18061 gaaagaccgc cgtttgcttt ctcaaaatat ttgaaacgag aatctttctc tacaaattct
    18121 tcacatattt tggatgaatg atctggagag ccatcattga ttaataaaca ctcaaaattt
    18181 tgatacgtct gattctgaat gctatccaaa cacattcgca aataattctc aacgttgtag
    18241 attgggacta caatactaat tagttcattt tccatatact aactttccta gacttgattt
    18301 attctatttc cctcttaatc tcttctcttt acaaaaagat ttccattatt ataaactaca
    18361 ctgtttgatg gaatatcatt gcgtatgaga caacctgctc caataatggt attatcccca
    18421 atcatcactc cttttaaaat cgtgacatta cttcctatcc agcagtctct gccaactcta
    18481 atcggcgctg tagtccactg ccacttttca atcttttcag cagtataaat atggtcgtga
    18541 tcataaaaac gaacaccctc tcccatcatg gtaccagctc cgatttcaat tctttccata
    18601 cagttaaaac tacaagagtt gttgataaaa acaccatcat gcagtattaa cttaccagaa
    18661 gagacatgac aattttcaaa atttctgcag atgacatttg ctcctacaat gaagtgagcc
    18721 ttatcatcga ttttaaagac attccaagtc ccatcttgga tgattttttc ttgatgattg
    18781 gtgcctactc tgacttcatt cagatagtct ctcactctag aaaccaattt atctggtgta
    18841 cttgatagaa aaacttcctg cccctgattt ccatggactg tattgtcaaa tgcaaagaca
    18901 gattttccag cctgtacaaa cttggagaca atagcatcta cttcttgaaa gtgattgata
    18961 tctagtaaaa agtcacaggt atcgactaga aagtttagct gttcttcatt tgtaatcgtt
    19021 cggagacgga cattatagtt cgtttctaag ctacggattt tctcggaaaa ctggacaggc
    19081 gctgcaatat gaaaacagat atttggcaag ttttcaagga gataatccaa ctcttcaatc
    19141 tgatcactaa ccgtcaaaat cagagcgtct cctactaaat gatttttctt catttgctta
    19201 tttctctcaa tttctcaatc attgcttgaa cttggtcaac agcatatgcc tcctgaccta
    19261 cttcataggt tttagtattt tcaaaggata agataggctt gccaagatta gcaaattgat
    19321 cgagaatttc ttctgtcttt tcaccatgat taatatctaa aagtacttga ctggtttcta
    19381 ccaattcatt atcgacatct accaaatagt gaattccgtt aaatatagtc acgtttggat
    19441 aaattgtcat ctgagccaat cgatcactaa ctattactct agctgctatc ttaaactgaa
    19501 tatcaggcaa ggattgaacc aatgtctcaa tttgttcaat atggtctgag gcagtataga
    19561 ttagacaagt gaaaggttcc tttattggat agatgtgaga tctttgtaat ggatgtaaat
    19621 gatggttttg tcccaattct gtccattcaa gcccatgata gtaccaccaa acttcacgat
    19681 aggtttgggc cgccaaatct ttccacggtt tccgatgaga aagatagtga ataatagcag
    19741 gataatcctg accctcaggc aattgataat cagcgaactg tttatgaatg acaatatgat
    19801 tataatcaaa gtccaattcc aaccatttat gttcaaaaag catattcaaa atactttgat
    19861 cagcctgttc caccttatta tgccattcat tagtcaaatc aattaatttt tgggtcatat
    19921 tctctttttt ccaaaaagca ttgtttacca agagaacacc ggcattaaag atttcttgac
    19981 caaaataagc tctgccccca aaatctctaa cagcagccaa aggataatct tgtaagtctg
    20041 tagcaaacaa atcatccaga tttttcgtta caagtaaatc acagtccaaa tagagggcct
    20101 tatcttcttg cacgaaatca gctatgaaat agcgtaaaaa aactgtgtaa ctaatatccg
    20161 ttttataaca tgaaatttgc tcagaagtta cccgacaatt aataatttct gagtcaaact
    20221 tctctaagcg cttatttaat tgcttaatcc attcatttgg aaaatcgcta tgaatcaaat
    20281 aaaaacgaag cgagcgattg tggtaacaga tagacctgat tgtcgttaaa acttggtcta
    20341 catagccata gtttgctgcg aggacaatgg ctttcttttc actttcctct tgtctcgata
    20401 gctgatttaa aagcctttgt ttcatttcaa actctttata cgttgctgtg tcagataaac
    20461 cactagcttg accgttggcg agactgactt ccaacatctg acgataaact gccaagtgtt
    20521 tctctagagg ataacccata ttagctagta gcgtaatacg ttcagacata gcatcaacta
    20581 aggcgtgcat ccacttttct gtccaaactc ttgataaact accttttcta atccgataag
    20641 cataaagacc tttatttaaa taaattacct tttccgataa taaatatacc ttttgattga
    20701 ggtaaccata ttctcctaat ttacctatgt caaagcgcaa ctgctcaaac aatcttgcct
    20761 tatagagttt accccaagca gatatcaaag caaaactctt catttcttga gtttcataca
    20821 agttctcaaa gatggaaaca ttgtcatata ccttctcata ataggagtct cctgatatat
    20881 gaaagtagaa cattccttca ctttcgttga aagaataata attaccaact gcaatatcag
    20941 cctgatactc tactattttt ttatatagag tttctacata atcttgctca atccaatcat
    21001 ccgagtccac aaaggtcaca taatttccgg acatattatt cagaccggtg tttcgtgcgg
    21061 cagaaatacc agcattttct tgttctatat agagaattcg gtgatccatt tctgaaaatt
    21121 ctttacaaat ttcacctgaa gcatccgtag aaccatcatt aacgacaaca atctcaatat
    21181 ttttatatgt ttgagaaata atactatcta gacatttcct cagatagttt tctacattgt
    21241 atactggtac aatgaccgta attttatcat ccactagcaa ttttctcctt gatttatgtg
    21301 cgcgagttag tttagcctaa ctttacaggt tgtcttactc ttcttcatca cgtttacggc
    21361 gtttcgcaac caatccaata cctgtaacag ctgctagaac tccaagtaac acagatccaa
    21421 ttgacgactc agtacctgta ttaggcaatt ctttttggga tttacctgtc gatccagaag
    21481 catttccaac ttgtgagttc gaatggtttg ctgaatttga aactgatgta cttgctgaag
    21541 ctgacgcact ggttgatgct gactcagaag ctgatgtgct tactgaggct gaggcactgg
    21601 ttgacgctga agctgaggcg ctggttgatg ctgaggctga ggcgctagta cttgctgaag
    21661 ccgaggcact tgttgatgcg gaggctgatg cgctggttga tgccgattca gacgctgagg
    21721 tgcttgctga ggccgaagca ctcgttgatg ctgaagctga ggcactggtg cttgctgaag
    21781 ccgacgcact cgttgatgct gaagccgacg cactggtgct tgctgaagct gacgcacttg
    21841 ttgatgcgga agccgaagca ctcgttgagg ctgaagctga cgcactggta cttgctgaag
    21901 ccgaggcgct tgtgcttgct gaagccgaag cactcgttga tgctgaagcc gaggcactcg
    21961 ttgatgctga agccgacgca ctcgttgatg ctgaggctga cgcactggta cttgctgaag
    22021 ccgaagcact ggttgaggcc gattcagacg ctgaggtact tgctgaagcc gacgcactcg
    22081 ttgatgcgga agctgacgca ctggttgacg ctgaagctga ggcgcttgtg cttgctgagg
    22141 ctgacgcact ggttgatgcg gattcagatg ctgatgtgct tgctgaggat ga
//
LOCUS       DAVHEW010000025        21463 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVHEW010000025 DAVHEW010000000
VERSION     DAVHEW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.025"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            81..302
                     /locus_tag="VVL53_000881"
     CDS             81..302
                     /locus_tag="VVL53_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183077.1"
                     /translation="MYRRLRDLREDHDLPQKQIATILSFTNSAYAKIERGEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            749..1051
                     /locus_tag="VVL53_000882"
                     /pseudo
     CDS             749..1051
                     /locus_tag="VVL53_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1207..2436
                     /locus_tag="VVL53_000883"
     CDS             1207..2436
                     /locus_tag="VVL53_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU4183078.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2456..5152
                     /gene="ppc"
                     /locus_tag="VVL53_000884"
     CDS             2456..5152
                     /gene="ppc"
                     /locus_tag="VVL53_000884"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU4183079.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYETIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5235..5452
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5565..6569
                     /locus_tag="VVL53_000885"
     CDS             5565..6569
                     /locus_tag="VVL53_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4183080.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6638..7504
                     /locus_tag="VVL53_000886"
     CDS             6638..7504
                     /locus_tag="VVL53_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183081.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQNVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7501..8259
                     /locus_tag="VVL53_000887"
     CDS             7501..8259
                     /locus_tag="VVL53_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183082.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8349..10109
                     /gene="dnaG"
                     /locus_tag="VVL53_000888"
     CDS             8349..10109
                     /gene="dnaG"
                     /locus_tag="VVL53_000888"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU4183083.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10112..11221
                     /gene="rpoD"
                     /locus_tag="VVL53_000889"
     CDS             10112..11221
                     /gene="rpoD"
                     /locus_tag="VVL53_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU4183084.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11235..11564
                     /locus_tag="VVL53_000890"
     CDS             11235..11564
                     /locus_tag="VVL53_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU4183085.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11679..12722
                     /gene="cpoA"
                     /locus_tag="VVL53_000891"
     CDS             11679..12722
                     /gene="cpoA"
                     /locus_tag="VVL53_000891"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU4183086.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12738..14063
                     /locus_tag="VVL53_000892"
     CDS             12738..14063
                     /locus_tag="VVL53_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4183087.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14094..14270
                     /locus_tag="VVL53_000893"
     CDS             14094..14270
                     /locus_tag="VVL53_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU4183088.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14329..14463
                     /locus_tag="VVL53_000894"
     CDS             14329..14463
                     /locus_tag="VVL53_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU4183089.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14527..15837
                     /gene="obgE"
                     /locus_tag="VVL53_000895"
     CDS             14527..15837
                     /gene="obgE"
                     /locus_tag="VVL53_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU4183090.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15847..16005
                     /locus_tag="VVL53_000896"
     CDS             15847..16005
                     /locus_tag="VVL53_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183091.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17148..18407
                     /locus_tag="VVL53_000897"
     CDS             17148..18407
                     /locus_tag="VVL53_000897"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4183092.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18423..18974
                     /locus_tag="VVL53_000898"
     CDS             18423..18974
                     /locus_tag="VVL53_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183093.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18967..20244
                     /locus_tag="VVL53_000899"
     CDS             18967..20244
                     /locus_tag="VVL53_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4183094.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20260..21120
                     /locus_tag="VVL53_000900"
     CDS             20260..21120
                     /locus_tag="VVL53_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU4183095.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6557 a         3690 c         4818 g         6398 t
ORIGIN      
        1 aaaattcctg ttgtccgatt tggacaatat cctaaatagt tacaatatat ggtctatact
       61 ttttcttagg agaaagctag atgtacagac gtttgagaga tttgagggag gatcatgatc
      121 tgccccaaaa gcaaatagct acaatacttt cgtttacaaa ttcagcttat gccaaaattg
      181 aacggggtga gcatgcgttg acggctgatg tattggttaa actctcagat ttctatgacg
      241 tcagtacaga ctatttattg ggattaactg attttcctga taaaattcgc tttagaaaat
      301 aatctcctca atttcataga gtttgaaaat gagtgagatt ttttatttgc cctttgacaa
      361 ctgaatagcc taaaatggta ctttcctcat ttgtggagca aatttgaatg gctcgccatg
      421 ataagagcga ttttaaaatc atcaataaaa tagagcgata ctttatatgc catgatacaa
      481 atgatataca atgatacttc tgaccgttca gcctgccaac gtaaaagagc agcaagtgaa
      541 attcttatga tgacttcatc agtcatgcca cgttgaatgt gtgagtttgt tagataaacg
      601 caattaatcc tcaaaaggtt ccccgaacct tttgagttct acagacgcat cacgtggagt
      661 gtgtaagctt gttgctaaaa gcgtaaaaac cttggaacga aaggaataat agactttctg
      721 cgaaacaaaa atataataca ataaaactat gaatgatgaa gcaagtaaac aattgagcga
      781 tagccgtttc aagatccttg taggtgttca gcgcacgact tttgaagaga tgttagctgt
      841 gttaaaaaca gcttatcaac gtaaacgcgc aaaaggtgga cgaaaaagca aattaagcct
      901 agacgatctc cttatggtaa ctattcaata catgcgagaa tagagcactt atgaacaaat
      961 tgcggctgat tttggcattc acgaaagcaa cttaatccgt cggagtcaat gggttgaagc
     1021 aactcttatt caaaatggtt ttacgatttc aaattctgcc ttaattctgt aaaaacagta
     1081 aaattcgaag gattgtaagg taagagtttt tttctttctg aaaaaatggt ataatagcaa
     1141 tcaaaactag aaaataaaac ggaatttgga acagatttgt ctgtatccta gtagagtggt
     1201 gatactatga agattagtaa gaggcactta ttaaattatt ccatcttgat tccctacttg
     1261 cttttatcta ttttgggctt gattgtggtc tattcgacca ccagtgctat tttaattgaa
     1321 gaaggcaaga gcgccttgca gttggttcga aaccaaggaa tcttttggat tgttagtttg
     1381 atactgattg ccttaattta taaattgaga ctagattttt tgagaaatga gcgactaatc
     1441 attttagtta tattaataga aatgctttta ttgttcttgg ctcgttttat tggtatttcc
     1501 gtaaacgggg catacggttg gatttcggtt gcaggaataa ctattcagcc agctgagtac
     1561 ttaaaaatca ttattatttg gtatttagct caccgattct ccaaacagca agaagaaata
     1621 gctacttatg attttcaagt tttgactcaa aatcaatggc ttccccgtgc ttttaatgat
     1681 tggcgattcg ttctcctagt tctgattgga agtttgggaa ttttccctga tttaggaaat
     1741 gcgactattt tagtcttggt ttccttgatt atgtatacag ttagtggaat cgcttatcgc
     1801 tggttttcaa ccattctggc gctcgtatct gccgcttctg tctttgtctt gaccactatc
     1861 agcctaatcg gtgttgagac cttttcaaaa attccagtat tcggctatgt agccaagcgc
     1921 tttagtgcct tttttaatcc ttttgccgat cgtgctgatg caggtcacca gttagctaat
     1981 tcttattttg ccatggtcaa tggcggttgg tttggtctag gtcttggaaa ctcgattgaa
     2041 aaacgaggtt atttgccaga agctcataca gactttgtct tttctatcgt gattgaagaa
     2101 tttggctttg ttggtgccag tcttatttta gctctcttgt ttttcatgat tttgcggatt
     2161 atcttggtcg gtatccgagc ggagaatcct ttcaatgcca tggttgcact cggtgtcgga
     2221 gggatgatgt tggttcaggt atttgtcaat atcggaggga tttcgggctt gattccatct
     2281 acaggagtga ctttcccctt cttatcccag ggtggaaata gtcttctagt cttatcagtg
     2341 gcagtagcct ttgtcttaaa tattgatgcc agtgaaaaac gcgctaaatt gtaccgagaa
     2401 ttggaaaatc aaccaatgaa ccttctgttg aagtaggata aagaaaggat agtttatgtc
     2461 tcttcaaaaa ttagaaaatt atagtaataa aagtgttgtg caagaagaag tcttgattct
     2521 aacagaatta ctggaagata ttactaaaaa tatgcttgcc ccagagacct ttgaaaaaat
     2581 aatacagttg aaagaattat caacgcagga agattatcaa ggtctaaacc gtctagtgac
     2641 tagcttatca aatgatgaaa tggtctatat ttcacgctat ttctctatct tgcctctttt
     2701 gattaatatt tcagaggatg tggatttagc ttatgaaatc aatcatcaaa ataatattga
     2761 tcaggactat ttaggtaaat tatctacaac gattaaattg gtagcagaaa aggaaaatgc
     2821 cgttgagatc ctagaacact tgaatgttgt ccctgttttg acagcccatc caacacaagt
     2881 gcaacgcaaa agtatgttgg atttaacaaa tcatattcat agtcttttgc gtaaataccg
     2941 tgatgttaag ttggggttga tcaataaaga taaatggtac aatgatttgc gtcgttacat
     3001 cgaaattatc atgcagacag acatgattcg tgagaaaaaa ttaaaagtga ctaacgaaat
     3061 cacgaatgct atggaatatt ataacagctc ctttttgaaa gctgtacctc atttgacgac
     3121 ggagtataag cgcttagcgc aagcgcatgg tctgaattta aaacaggcta aaccaatcac
     3181 catgggtatg tggataggtg gtgaccgtga tggaaatcca tttgttacag caaagacctt
     3241 gaagcagtct gcactcactc agtgtgaagt catcatgaac tactatgata aaaagattta
     3301 ccaactttat cgtgaatttt ctctttcaac tagcattgtc aacgtcagca agcaagtcag
     3361 agaaatggct cgtcaatcca aggataactc gatttaccgc gaaaaagagc tttaccgtcg
     3421 tgccttgttt gatattcaat caaaaattca ggcaactaaa acctatctga ttgaggatga
     3481 agaagttggg actcgttatg aaaccgccaa tgatttctac aaggatttga ttgccattcg
     3541 agattctcta ctagaaaata agggcgagtc cttgatttca ggtgattttg tggaattatt
     3601 gcaggcagta gagatatttg gtttttactt agcatcaatt gatatgcgac aagactctag
     3661 cgtctatgaa gcctgtgtgg cagaactctt gaaatcagca ggaattcatt ctcgttatag
     3721 cgagttgagc gaagaagaaa agtgtgacct tctcttgaaa gaattagaag aagatccccg
     3781 aattctttct gcgactcacg cagaaaaatc agaattatta gcaaaagaat tagctatttt
     3841 taagacggct cgtgttttga aagataagtt gggagatgat gtcatccgtc agaccatcat
     3901 ttcacatgca accagccttt ctgatatgct agaattagct attctgttaa aagaagtagg
     3961 actggtggat acggaaaggg cgcgtgttca gattgttccc ctttttgaaa caattgaaga
     4021 cttggatcat tcagaggaaa caatgagaaa atatctttct cttagccttg ccaaaaaatg
     4081 gattgactca cgaaataact accaagaaat catgcttggc tactctgaca gtaataaaga
     4141 tggcggttac ttgtcatcat gttggaccct ctacaaggct caacaacaat tgactgctat
     4201 tggagatgaa tttggcgtta aggttacctt cttccatggt cgtggtggta ctgtcggtcg
     4261 tggtggtggg ccaacctatg aagccattac atctcaaccg ctcaagtcta tcaaggatcg
     4321 tatccgcttg acggagcagg gtgaagtaat tgggaataaa tacggtaaca aagacgccgc
     4381 ttactataac cttgaaatgc tagtatcggc agctattaac cgtatgatta ctcagaagaa
     4441 gagcgatacc aataccccaa atcgttatga aaccattatg gatcaagtag tggaccgtag
     4501 ttacgatatc taccgtgatt tggtctttgg taatgagcat ttctatgatt atttcttcga
     4561 gtcaagtcca atcaaggcta tttcaagttt taatattggt tctcgtccag ccgctcgtaa
     4621 gactattact gaaatcggtg gtttgcgtgc catcccttgg gtattctcat ggtcacagag
     4681 tcgtgttatg ttccctggat ggtacggggt tggttcaagc ttcaaggaat ttatcaataa
     4741 aaatccagag aatattgcta tcttacgaga tatgtaccaa aattggcctt tcttccaatc
     4801 gcttctttca aatgttgata tggttttgtc aaaatcaaat atgaatattg cttttgaata
     4861 tgctaaactt tgtgaagacg agcaagttaa ggccatctat gagactattt taaatgaatg
     4921 gcaagttact aagaacgtta tcttggctat tgaaggacat gacgaactct tagctgacaa
     4981 tccatatcta aaagctagtc tggattaccg tatgccttac tttaatattc tcaactatat
     5041 tcagttggag ttgattaaac gccaacgtcg tggagaattg tccagtgatc aagaacgatt
     5101 gattcatatc accatcaacg gaattgcgac aggattgcgt aattcaggtt gataattttc
     5161 aagagtgaat gctaaaagtg aatatcaaaa aaattctaat agactattga caagtagttt
     5221 aaaaatgata taatttaacc attcagaaaa gtaatcatac aaacttttta gagagtctgt
     5281 ggtagctgaa aacagataag tggcaatgat gaaaattggg ctgaatgcta tttagaattt
     5341 gaaattataa aaattcggta agcacacctt acagtgcatc tcgttattgc gagactgagc
     5401 gatagggaaa ttccctataa ttgaggtggt accgcgcatc gacgtcctca cacaagtttt
     5461 ttgtgtgagg atttttttga tggaggttag tatggaaaga aaacgatggc gtcgcttgtt
     5521 tagataagtg aaatatgtta aaggaaataa aaaggagaaa cagaatgaaa aataaacgtt
     5581 taattggaat tattgctgca ttagcagtct tagtagcagg aagcttgatt tattcttcaa
     5641 tgaataaatc agaagctcag aataataagg atgagaagaa aataaccaag attggtgtgc
     5701 ttcaatttgt gagccatcca tcccttgatt tgatttataa agggatccaa gatggacttg
     5761 cagaagaagg atataaagat gatcaagtta aaattgattt tatgaactca gaaggtgacc
     5821 aaagtaaggt tgcgacaatg agtaaacaat tggttgcaaa tgggaatgac cttgtggttg
     5881 gtatcgcaac accagcagcc caagggttgg ctagtgcaac aaaagaccta ccggttatca
     5941 tggccgctat tacagaccca attggtgcta acttggttaa agatttgaaa aaaccaggtg
     6001 gcaacgttac aggggtatct gaccacaatc cagctcaaca acaagttgaa ctcatcaagg
     6061 ctctgacacc gaatgtgaaa acaatcggag ctctttactc aagtagcgaa gacaattcaa
     6121 aaacacaggt cgaagaattt aaggcttatg ctgaaaaagc aggtctgaca gtggaaacat
     6181 ttgcagttcc ttcaacaaat gaaattgcct caactgtcac tgttatgact agcaaggtag
     6241 atgctatttg ggttccaatt gataacacca ttgcatcagg atttccaacg gttgtctcta
     6301 gcaatcaaag ttctaagaaa ccaatttatc ccagtgcgac agctatggta gaagtaggtg
     6361 gtttggcatc agttgtaatt gaccaacatg accttggtgt ggcaacaggt aaaatgattg
     6421 tgcaagtctt gaaaggtgca aaaccagccg ataccccagt caatgtcttt tcaactggta
     6481 agtcagtcat caataaaaaa atagcacaag aactaggtat tactattcct gagtctgttc
     6541 tcaaagaagc aggacaagtc atcgaataaa taaggtaggg aggaggagac tcttcccatt
     6601 ttttaaaaag atgaatatag aaaggattaa ataaaatatg attgtttcca ttatttctca
     6661 aggatttgtc tgggctattc taggtctggg aatctttatg acatttagga ttttaaactt
     6721 tccagatatg acgacagaag gttccttccc tcttggggga gctgttgctg tcactttgat
     6781 aaccaaaggc gtgaacccat ttttagcgac acttgttgct gtaggagcag gttgtttggc
     6841 tggaatggca gcaggccttc tttatacaaa agggaagatc ccaaccttgc tctcagggat
     6901 tttggtgatg acttcttgtc actcaatcat gctcttgatt atgggacgtg cgaatttagg
     6961 cctgcttgga accaagcaaa ttcagaatgt tttgcctttt gattcggatt tgaatcaact
     7021 cttgacaggt ctcatctttg tgagtattgt tattgctctc atgctctttt tcttggacac
     7081 taaactcgga caagcctata ttgctacagg ggataatcct gatatggcta gaagtttcgg
     7141 gattcatact ggacgcatgg agctcatggg cttggtctta tcaaatggtg tgattgccct
     7201 tgcaggtgcc ctcattgctc agcaagaagg ttatgccgat gtgtctcgag ggatcggggt
     7261 tatcgttgtg gggcttgcaa gtttgattat tggagaagtt attttcaaga gtttgagctt
     7321 ggcagagcgt ttggttacta tcgttgtagg ttctatcgct tatcaatttt tagtgtgggc
     7381 agttatcgca cttggcttta atacaagtta ccttcgttta tacagtgcct tgattttagc
     7441 agtctgcctc atgattccaa catttaagca aacaatcttg aaaggagcca agttaagcaa
     7501 atgacagcaa ttgtagaatt aaaaaatgca accaaaatcg ttaaaaatgg ctttgatgaa
     7561 gaaaagatta ttttaaatga tgtttcctta gaaatttttg aacgggactt tatcacgatt
     7621 ttgggcggaa atggtgctgg aaaatcaact ctctttaaca ctatagcagg gaccttatca
     7681 ctaactagtg gaactatccg tattttaggt gaagatctca ctaagttttc acccgagaag
     7741 cgtgccaagt acctgtctcg tgtcttccaa gatccaaaga tggggacagc tccccgtatg
     7801 acggtcgctg aaaatctttt aatcgccaag tttcgtggtg aaaagcgtgg attgttacca
     7861 cgacgcttga ctagctataa ggatgaattt caggcaacca ttgaaaaagt aggaaatggt
     7921 cttgagaaac acttgaatac accgattgag ttcttatcag gtggacaaag acaggctttg
     7981 agtctcttga tggcaacctt gaagcgacct gaattactcc tgttagatga gcatactgct
     8041 gccctggatc caaagactag tgttgctttg atggaattga cagatgaatt tgttaagaaa
     8101 gatcagctaa cagcccttat gattactcat catatggaag atgctctcaa atacggcaat
     8161 cgcttgattg tcatgaaaga aggacgaatt atccaagatt taaaccaaga agaaaaagca
     8221 aaaatgaaaa tctctgatta ttatcaactc tttgaataaa gtagggaaaa tgagtgaaat
     8281 ggtttacttt ttttctgaaa taaagtatac tatataaagt aaactatgat aacatggagg
     8341 tattgtgtat ggttgacaaa caagtcattg aagaaatcaa aaacaatgcc aacattgtgg
     8401 aagtcatagg agatgtgatt tctttacaaa aggcaggacg gaactatcta gggctctgtc
     8461 cttttcatgg tgaaaaaaca ccatctttca atgttgtaga ggacaagcag ttttatcact
     8521 gtttcggttg tggtcgctca ggtgatgtct ttaagttcat cgaggagtac caaggggttc
     8581 cctttataga agcagtccaa atcttaggtc agcgtgttgg aattgaggtt gaaaaaccgc
     8641 tttatagtga gcagaagtca gcctcacctc accaagctct ttatgatatg cacgaagatg
     8701 cagccaaatt ttaccatgct attctcatga caacgactat gggcgaagag gccagaaatt
     8761 acctttatca gcgggggttg acagatgaag tgcttaaaca tttttggatt ggtttagcac
     8821 ctccagaacg aaactatctc tatcaacgtt tgtctgatca gtatcgtgaa gaggatttac
     8881 tggattcagg cctgttttat ctctcggatg ccaatcaatt tgtagacacc tttcacaatc
     8941 gcattatgtt tcccctgaca aatgaccaag gaaaggtcat tgccttctca ggtcgtatct
     9001 ggcaaaaaac ggattcacaa acttctaagt ataaaaacag ccgatcgact gcaattttta
     9061 acaaaagtta cgaattatat catatggata gggcaaaaag atcttctgga aaagctagtg
     9121 agatttacct gatggaagga ttcatggatg ttattgcagc ctatcgggct ggaatcgaaa
     9181 atgctgtggc gtcgatggga acagccttga gtcgagagca tgttgagcat ctgaaaaggt
     9241 taaccaagaa attggttctt gtttacgatg gagataaggc tgggcaagcc gcgacattga
     9301 aagcattgga tgaaattggt gatatgcctg tgcaaatcgt cagcatgcct gataacttgg
     9361 atcctgatga gtatctacaa aaaaatggtc cagaagactt ggcctatcta ttaacgaaaa
     9421 ctcgtattag tccgattgag ttctacattc atcagtacaa acctgaaaac agtgaaaatc
     9481 tgcaggctca gattgagttt cttgaaaaaa tagctccctt gattgttcaa gaaaagtcca
     9541 tcgctgctca aaacagctat attcatattt tagctgacag tctggcgtcc tttgattata
     9601 cccagattga gcagattgtt aatgagagtc gtcaggtgca aaggcagaat cgcatggaag
     9661 gaatttccag accgacgcca atcaccatgc ctgtcaccaa gcagttatcg gctattatga
     9721 gggcagaagc ccatctactc tatcggatga tggaatcccc tcttgttttg aacgattacc
     9781 gtttgcgaga agactttgca tttgctacac ctgaatttca ggtcttatat gacttgcttg
     9841 gccagtatgg aaatcttcct ccagaagttt tagcagagca gacagaggaa gttgaaagag
     9901 cttggtacca agttttagct caggatttgc ctgctgagat atcgccgcag gaacttagtg
     9961 aagtagagat gactcgaaac aaggctctct tgaatcagga caatatgaga atcaaaaaga
    10021 aggtgcagga agctagccat gtaggagata cagatacagc cctagaagaa ttggaacgtt
    10081 taatttccca aaagagaaga atggagtaat aatggcaaca aaacaaaaag aagtaacaac
    10141 atttgacgta caggtagcag aatttatccg taatcataag caaaaaggga cagcaacaga
    10201 tgatgaaatc aatgcaagtc tggttattcc ttttaccttg gacgctgatg ggattgaaga
    10261 tctcttgcaa cggattcagg atgcaggcat ttctatcaca gataacgaag gaaatccaag
    10321 tgcgcgtgtt ctcagcaatg aagaagaacc agaactcagc gatgaggact tgattgggtc
    10381 aacttctgct aaggtcaatg accctgtccg tatgtacttg aaagaaatag gggtcgttcc
    10441 tctcttgacc aatgaagagg agaaagagtt ggcactggct gttgaagctg gtgatatcga
    10501 agccaaacaa cgtcttgcgg aagccaatct tcgtttggtt gtttccattg ccaaacgcta
    10561 tgtcggtcgt ggtatgcagt tccttgactt gattcaagaa ggaaatatgg gcttgatgaa
    10621 ggcggttgac aagtttgact attctaaagg gttcaagttt tcaacttatg caacttggtg
    10681 gattcgtcag gctatcactc gtgctattgc ggaccaagct cgtaccatcc gtatcccagt
    10741 tcacatggtt gaaactatca ataaattggt tcgtgaacag cggaatctcc ttcaagaatt
    10801 ggggcaagat ccgacaccag aacagattgc tgaacgaatg gatatgacac ctgataaggt
    10861 tcgtgaaatc ttgaagattg cccaagaacc agtatctctt gaaactccta tcggtgaaga
    10921 ggacgatagc caccttggag actttatcga agatgaagtg attgaaaatc cagtggatta
    10981 tacgactcgt atcgtcttgc gtgagcaatt ggatgaaatc ttagatactc ttacagaccg
    11041 tgaagaaaat gttctgcgtc tacgttttgg actagatgat ggaaaaatgc gcacacttga
    11101 agatgtgggg aaagtcttta acgtaactcg tgagcgtatc cgtcagattg aagcaaaggc
    11161 tttgagaaaa ctacgccaac caagtcgtag caaaccgctt cgtgatttta ttgaagacta
    11221 agagtgagga aaatatggct tatacagaag agcaaattga aaacatcaaa acacggattt
    11281 taacagcctt ggaagaagtc atcgaccctg agttgggaat cgatattgtc aatcttggtt
    11341 tgatctatga gattcgtttt gacggtgaca cagggcaaac agagattgac atgactttga
    11401 caactatggg ttgtccctta gcagaccttt tgacagacca gatttatgat gctatgatag
    11461 aggttccaga agtaacggat actgaggtta aattagtctg gtatccagcc tggacagttg
    11521 aaaaaatgag tcgctatgcc cgcattgccc taggcattaa gtaaacatat aaaagcatgt
    11581 gagagactgt tggaaaagcg aggaaatttc ccctcttttc ctctagtctc tcctttcttt
    11641 tgctgatttt attcaaagaa aatgatataa tagtagttat ggagaaaaag aaattacgca
    11701 tcaatatgtt gagttcaagt gagaaagtag caggacaggg agtttcaggt gcttaccgtg
    11761 aattagttcg tcttcttcac cgtgctgcca aggaccaatt gattgttaca gaaaatcttc
    11821 caatcgaggc agatgtgact cactttcata cgattgattt tccctattat ttatcaacct
    11881 tccaaaagaa acgctcaggg agaaagattg gctatgtgca tttcttgcca gctacacttg
    11941 agggaagttt gaaaattcca tttttcttaa agggaattgt gaaacgctat gtattttctt
    12001 tttacaaccg gatggagcac ttggttgtgg tcaatcctat gtttattgag gatttggtag
    12061 cagctggtat tccacgtgaa aaagtgacct atattcctaa ctttgtcaac aaggaaaaat
    12121 ggcatcctct accacaagaa gaggtagtca gactgcgcac agatcttggt cttagtgaca
    12181 atcagtttat cgtagtaggt gctgggcaag ttcagaaacg taaagggatt gatgacttta
    12241 tccgtctggc tgaggaattg cctcagatta cctttatctg ggctggtggc ttctcttttg
    12301 gtggtatgac agatggttat gaacactata agaaaattat ggaaaatccc cctaaaaatt
    12361 tgatttttcc aggcattgta tcgccagagc ggatgcgcga attgtatgct ctagcggatc
    12421 ttttcttgtt gcctagttac aatgagctct ttcctatgac tattttagaa gctgcgagtt
    12481 gtgaggctcc tattatgttg cgtgatttag atctctataa ggtgattttg gagggaaatt
    12541 atcgggcgac agcgggtaga gaagagatga aagaggctat tttggaatat caagcaaatc
    12601 ctgctgtctt aaaagatctc aaagaaaagg ctaagaatat ttccagagag tattctgaag
    12661 agcatctgtt acaaatctgg ttggactttt atgagaaaca agccgcttta gggagaaagt
    12721 aaaaagtgag gtaatctatg cgaattggtt tatttacaga tacctatttt cctcaggttt
    12781 ctggtgttgc gaccagtatt cgaaccttga aaacagaact tgaaaagcag ggacatgctg
    12841 tttttatctt tacgacgaca gataaggatg tcaatcgcta cgaagattgg caaattatcc
    12901 gcattccaag tgttcctttc tttgctttta aggatcgtcg ctttgcctac cgaggtttta
    12961 gcaaggcact tgaaattgct aaacagtatc agctagatat tatccatact cagacagaat
    13021 tttctcttgg cctgttgggg atttggattg cgcgtgaatt gaaaattcca gtcatccata
    13081 cctatcacac ccagtatgaa gactatgtcc attatattgc taaggggatg ttgatccggc
    13141 cgagtatggt caagtatctg gttagaggtt tcctgcatga tgtggatggg gttatttgcc
    13201 ctagtgagat tgtccgtgac ttgctatctg attataaggt caaggttgaa aaacgggtca
    13261 ttcctactgg gattgaatta gccaagtttg agcgtccgga aatcaagcag gaaaatttga
    13321 aagaactgcg tagtaaacta gggattcaag atggtgaaaa gacgttgctt agtctttcga
    13381 gaatctccta tgaaaaaaat attcaagcag ttttagcagc ctttgctgat gttctgaaag
    13441 aggaagacaa ggttaaactg gtagtagctg gggatggccc ttatctgaat gacctcaaag
    13501 agcaagccca gaacctagag attcaagact cagtcatctt tacagggatg attgctccta
    13561 gtgagacggc tctttactat aaagcggcgg atttcttcat ttcggcatcg acaagcgaaa
    13621 cgcaaggttt gacctacttg gaaagcttag ccagtggaac acctgtcatt gctcacggaa
    13681 atccttattt gaacaacctc atcagtgata aaatgtttgg aaccttgtac tatggagaac
    13741 atgatttggc tggtgctatt ttggaagccc tgattgcaac accagacatg aacgagcata
    13801 ccttatcaga gaaattgtat gagatttcag ctgagaactt tgggaaacga gtgcatgagt
    13861 tttatctgga tgccattatt tcaaataact tccagaaaga tttggctaaa gatgatacgg
    13921 tcagtcagcg tatctttaag acagttttgt atcttcagca acaggtggtt gctgtacctg
    13981 taaaaggatc tagacgcatg ttgaaggctt caaaaacaca gttgatcagt atgagagact
    14041 attggaaaga ccatgaagaa tagaaagagg aacagctatg aaaaaaacaa ttaatgagaa
    14101 gcggtcgtga taaaaagatt gcgggtgttt gtgctggggt ggcccattat ctggatatgg
    14161 atccgactat cgttcaagtc atttggggtg ttcttacttg ctgttacgga gctggaattg
    14221 tagcttacat tattttatgg attatcgcac cagtagcaac tgactattga aaactagctc
    14281 aattcatgct aagataatag aaaatagaat gtaacgaagg agagaaaaat ggcatttgga
    14341 gataatggaa atcgtaaaaa aactatgttt gagaaaataa ccttgtttat cgtgattatc
    14401 atgctagtag caagtttatt gggaattttt gcaactgcaa ttggtgccct cagtaatcta
    14461 taaaatagat tcaagaaaat ttagtgactg ggatttccca gccctttttt aaagtgagaa
    14521 gaaataatga gtatgttttt agatacagct aagattaagg tcaaggctgg taatggtggc
    14581 gatggtatgg ttgcctttcg tcgtgaaaaa tatgtcccta atggaggccc ttggggtggt
    14641 gatggtggtc gtggaggcaa tgtggtcttc gttgtagacg aaggactacg taccttgatg
    14701 gatttccgct acaatcgtca tttcaaggct gattctggtg aaaaagggat gaccaaaggg
    14761 atgcatggtc gtggtgctga ggaccttaga gttcgagtac cacaaggtac gactgttcgt
    14821 gatgcggaga ctggcaaggt tttaacagat ttgattgaac atgggcaaga atttatcgtt
    14881 gcccacggtg gtcgtggtgg acgtggaaat attcgtttcg cgacaccaaa aaatcctgca
    14941 ccggaaatct ctgaaaatgg agaaccaggt caggaacgtg agttacaatt ggaactaaaa
    15001 atcttggcag atgtcggttt agtaggattc ccatctgtag ggaagtcaac acttttaagt
    15061 gttattacct cagctaagcc taaaattggt gcctaccact ttaccactat tgtaccaaat
    15121 ttaggtatgg ttcgcaccca atcaggtgaa tcctttgcag tagccgactt gccaggtttg
    15181 attgaagggg ctagtcaagg tgttggtttg ggaactcagt tcctccgtca catcgagcgt
    15241 acacgtgtta tccttcacat cattgatatg tcagctagcg agggccgtga tccatatgag
    15301 gactacctag ctatcaataa agagctggag tcttacaatc ttcgcctcat ggagcgtcca
    15361 cagattattg tagctaataa gatggacatg cctgagagtc aggaaaatct tgaagacttt
    15421 aagaaaaaat tggctgaaaa ttatgatgaa tttgaagagt taccagctat cttcccaatt
    15481 tctggattga ccaagcaagg tctggcaaca cttttagatg ctacagctga attgttagac
    15541 aagacaccag aatttttgct ctacgacgag tccgatatgg aagaagaagc ttactatgga
    15601 tttgacgaag aagaaaaagc ctttgaaatt agtcgtgatg acgatgcgac atgggtactt
    15661 tctggtgaaa aactcatgaa actctttaat atgaccaact ttgatcgtga tgaatctgtc
    15721 atgaaatttg cccgtcagct tcgtggtatg ggggttgatg aagcccttcg tgcgcgtgga
    15781 gctaaagatg gggatttggt ccgcattggt aaatttgagt ttgaatttgt agactaggag
    15841 actggtatgg gagataaacc gatatctttc cgagatgcgg atggtaattt tgtttccgcc
    15901 gcagacgttt ggaatgaaaa gaaattggaa gaactattta atcgtctcaa tccaaatcgt
    15961 gccttgagat tggcacgaac taaaaaggaa aatccatctc agtaaagaag ctaaaaaatc
    16021 ccgtgcctca tcagacacgg gattttgtgg tacgacaggc atgtatagca aactgaatct
    16081 ggaatagcac agcatatctt ctaaaatata gtaaaatgaa atgagaacag gacaaatcga
    16141 tcaggacagt aaaatcgatt tctaacaatg ttttagaagc agagatgtac tattctagtt
    16201 tcaatcaact atattgttat aaattgattt gaatttcaaa attaaattgt ttgattctta
    16261 tttcaatttg ttatagtata tctgatgtca aagttctcgg cgagtcaaat agcgattccc
    16321 aagcctgact atcgtgaggt agcggattaa aatggtctgg ggatagaccg ttttaagtct
    16381 gacgctggaa ataagaattg tcagaagaag ggatagcgaa atcgtggctc tacgaacagg
    16441 aacgtgataa taaggcgtat atagcggata agagggcatc aaactctaaa gtccaaaaag
    16501 gtagtcgtaa cctatatgcg taaatcacga gagtaattga attcgtacta agattttcta
    16561 ttttcactgt aaccttttaa cgcccttata tcttgtatac acgaggaaag atgtacgact
    16621 tatcccgtga ggtctatcac tataaagaga aaacgacaga tagaagtgat cctgagtcac
    16681 ggttatctgt ctgataggac ggtatgtata aaacgcttct gtgaactgag agaaggggga
    16741 gaagttcttg ctaaaattta gttgaacagc cgtattccga tacttagata agagatctag
    16801 tcttagctcc tactcagttt taggggataa aaaaggggca atagcgattc gagaaagatt
    16861 atactcttcg aaaatctctt caaatcacgt caatatcgcc ttgtcgtatg tgtaggatac
    16921 tgactacgtc agttccatct acaacctcaa aacagtgttt tgagcaacct gcggctagtt
    16981 tcctagtttg atctttgatt ttcattgagt attagtaatt cagttactaa ctcgtcaact
    17041 ctgatttatc caataaaatt gaaaaggatg gaaaaaagga taaatttatg atatacttta
    17101 ttttgaagac cttattagaa atcttgaaag agtattgaaa acttagaatg agaaaaattg
    17161 ttatcaatgg tggattacca ctgcaaggtg aaatcactat tagtggtgct aaaaatagtg
    17221 tcgttgcctt aattccagct attatcttgg ctgatgatgt ggtgactttg gattgcgttc
    17281 cagatatttc ggatgtagcc agtcttgtcg aaatcatgga attgatggga gctactgtta
    17341 agcgttatga cgatgtattg gagattgacc caagaggtgt tcaaaatatt ccaatgcctt
    17401 atggtaaaat taacagtctt cgtgcatctt actattttta tgggagcctc ttaggccgtt
    17461 ttggtgaagc gacagttggt ctaccgggag gatgtgatct tggtcctcgt ccgattgact
    17521 tacaccttaa ggcgtttgaa gctatgggtg ccactgctag ctacgaggga gataacatga
    17581 agttatctgc taaagataca ggacttcatg gtgcaagtat ttacatggat acggttagtg
    17641 tgggagcaac gattaatacg atgattgctg cggttaaagc aaatggtcgt actattattg
    17701 aaaatgcagc ccgtgaacct gagattattg atgtagctac tctcttgaat aatatgggtg
    17761 cccatatccg tggggcagga actaatatca tcattattga tggtgttgaa agattacatg
    17821 ggacacgtca tcaggtgatt ccagaccgca ttgaagctgg aacatatata tctttagctg
    17881 ctgcagttgg taaaggaatt cgtataaata atgttcttta cgaacacctg gaagggttta
    17941 ttgctaagtt ggaagaaatg ggagtgagaa tgactgtatc tgaagacagc atttttgtcg
    18001 aggaacagtc taatttgaaa gcaatcaata ttaagacagc tccttaccca ggctttgcaa
    18061 ctgatttgca acaaccgctt acccctcttt tactaagagc gaatggtcgt ggtacaattg
    18121 tcgatacgat ttacgaaaaa cgtgtaaatc atgtttttga actagcaaag atggatgcgg
    18181 atatttcgac aacaaatggt catattttgt acacgggtgg acgtgattta cgtggggcca
    18241 gtgttaaagc gaccgactta agagctgggg ctgcactagt cattgctggg cttatggctg
    18301 aaggtaaaac tgaaattacc aatatcgagt ttatcttacg tggttattct gatattatcg
    18361 aaaaattacg taatttagga gcggatatta gacttgttga ggattaaacc gtagaggtgt
    18421 ttatgaatat ttggaccaaa ttagcaatgt tttctttttt tgaaacggat cgcttgtatt
    18481 tgcgtccttt cttttttagt gatagtcagg acttccgcga gatagcttca aatccagaaa
    18541 atcttcaatt tattttccca acgcaggcaa gtctggaaga aagtcaatat gcactggcca
    18601 attactttat gaagtcccct ttgggagtgt gggcaatttg tgaccagaaa aatcaacaaa
    18661 tgattggttc tattaaattt gagaagttag atgaaatcaa aaaagaagct gagcttggct
    18721 attttttgag aaaagatgct tggtcgcaag gatttatgac agaggttgtt agaaaaattt
    18781 gtcagctttc ttttgaggaa tttggcttaa aacaattatt tatcattacc caccttgaaa
    18841 ataaagctag ccaaagagtt gctcttaagt ctggatttag tttgttccgt cagtttaagg
    18901 gaagtgatcg ttacacaaga aaaatgcggg attatcttga atttcggtat gtaaaaggag
    18961 agttcaatga gtaagcatca ggaaattcta agctatttgg aggaattacc agtaggtaaa
    19021 agggtcagtg ttcgtagcat ttcgaatcat ctaggagtta gtgatggaac agcctatcgg
    19081 gctattaaag aagctgaaaa ccgtggaatt gtggagaccc gtcctagaag tggaacaatt
    19141 cgtgttaaat cccagaaagt tgctatagag agattaacgt ttgctgaaat tgcagaagtg
    19201 acttcttctg aggttctggc tgggcaagaa ggtttagaga gagaatttag taagttttca
    19261 attggtgcca tgactgaaca aaatatcttg tcttaccttc atgatggggg gctcttgatt
    19321 gtcggagacc gaacccgtat tcagttgcta gccttggaaa atgaaaatgc agttctggtt
    19381 acagggggat ttcaggttca tgatgatgtg cttaaactgg ccaatcaaaa agggattcct
    19441 gttctaagaa gtaagcatga tacctttacc gtcgcgacca tgatcaataa agccttgtca
    19501 aatgtccaaa tcaagactga tattctgaca gttgagaaac tttatcgccc tagtcatgag
    19561 tatggttttc tgagagagac agatacagtt aaagattatt tggacttggt tcgtaagaat
    19621 cgtagcagcc gtttccctgt tatcaatcaa catcaggtcg ttgttggtgt tgtaaccatg
    19681 agagacgctg gtgataaatc accaagcacg acaattgata aggttatgtc tcgtagtcta
    19741 tttttggttg gattatcgac aaatattgcc aatgtgagtc aacggatgat cgcagaagac
    19801 tttgaaatgg taccagttgt tcgaagcaat caaactttgc ttggcgttgt gacgcgacga
    19861 gatgtcatgg agaagatgag ccgttcccaa gtttcggctc taccaacttt ttctgagcag
    19921 attggacaaa agctctctta tcaccatgat gaagtagtca ttacagtgga accctttatg
    19981 ctagaaaaaa atggagtttt ggctaatggt gtattggcag aaattctgac ccacatgacc
    20041 caagatttag ttgttaatag tggtcgcaat ctcattatcg agcagatgct gatctacttt
    20101 ttgcaggctg ttcagataga tgatatattg cgcattcagg cacggattat tcatcatacg
    20161 agacggtcag ctataattga ttacgatatt tatcatggtc accagattgt ttcaaaagca
    20221 aatgtgactg ttaaaattaa ttagaaacta ggagaaaaga tgataacatt aaaatcagct
    20281 cgtgaaatcg aagctatgga caaggctggt gattttctag caagtattca tataggctta
    20341 cgtgatttga ttaagccagg cgtagatatg tgggaagttg aagaatatgt ccgccgtcgt
    20401 tgtaaagaag aaaatttcct tccacttcag attggggttg acggtgccat gatggactat
    20461 ccttatgcta cctgttgctc tcttaacgat gaagtggctc acgctttccc tcgtcattat
    20521 atcttgaaag atggtgattt gctcaaagtt gatatggttt tgggaggtcc cattgctaaa
    20581 tctgacctaa atgtctcaaa attaaacttc aacaatgttg aacaaatgaa aaaatacact
    20641 cagagctatt ctggtggttt agcagactca tgttgggctt atgctgttgg tacaccgtcc
    20701 gaagaagtca aaaacttgat ggatgtaacc aaagaagcta tgtacaaggg tattgagcaa
    20761 gctgttgttg gaaatcgtat cggtgatatc ggtgcggcta ttcaagaata cgctgaaagt
    20821 cgtggttacg gtgtagtgcg tgatttggtt ggtcatggtg ttggcccaac tatgcacgaa
    20881 gaaccaatgg ttcctaacta tggtattgca ggtcgtggac tccgtcttcg tgaaggaatg
    20941 gtcttaacca ttgaaccaat gatcaataca ggcgattggg aaattgatac agatatgaaa
    21001 actggttggg cgcataagac cattgacggt ggattgtcat gtcagtatga acaccaattt
    21061 gtcattacga aagatggacc tgttatcttg actagccaag gtgaagaagg aacttattaa
    21121 taaaaagtga aaagactact ggaagtttat tttgataaaa aatccagtag atcttttcat
    21181 aataaaacgc attgtatcaa gtgttagggg ctgatatcat gcgtttttct gcttttaaga
    21241 ttttttccaa ctctgtttgt aagcgcatca taacaaaggg tctaggattc agggctctcc
    21301 tcctatatac tattagtaaa gtaaaactaa gggaggatat tttagtgtcg cagtctattg
    21361 ttcctgtaga gattccacaa tattgtcgtt ttgattctaa aaagagaaat ggaattctgt
    21421 ttaatgttcg tattgccaat cttaaattta cttttttata tta
//
LOCUS       DAVHEW010000026        20811 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVHEW010000026 DAVHEW010000000
VERSION     DAVHEW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.026"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            159..2141
                     /locus_tag="VVL53_000901"
     CDS             159..2141
                     /locus_tag="VVL53_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4183096.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2442..7745
                     /locus_tag="VVL53_000902"
     CDS             2442..7745
                     /locus_tag="VVL53_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU4183097.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTASTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMEALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQSSMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKMPDYKQEIA
                     RPETPKQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(7912..10071)
                     /locus_tag="VVL53_000903"
     CDS             complement(7912..10071)
                     /locus_tag="VVL53_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU4183098.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTDTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSLHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLVYGIGRGAYLPWLPQAGKTGTSNYTDE
                     EIEKYIKNTGYVAPDEMFVGYTRKYAMAVWTGYSNRLTPLVGDGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10068..10664)
                     /gene="recU"
                     /locus_tag="VVL53_000904"
     CDS             complement(10068..10664)
                     /gene="recU"
                     /locus_tag="VVL53_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU4183099.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10730..11257
                     /locus_tag="VVL53_000905"
     CDS             10730..11257
                     /locus_tag="VVL53_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU4183100.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            11327..11656
                     /gene="gpsB"
                     /locus_tag="VVL53_000906"
     CDS             11327..11656
                     /gene="gpsB"
                     /locus_tag="VVL53_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU4183101.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11677..12057
                     /gene="rnpB"
                     /locus_tag="VVL53_000907"
     ncRNA           11677..12057
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VVL53_000907"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12142..13299
                     /locus_tag="VVL53_000908"
     CDS             12142..13299
                     /locus_tag="VVL53_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU4183102.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13312..14706
                     /gene="mapZ"
                     /locus_tag="VVL53_000909"
     CDS             13312..14706
                     /gene="mapZ"
                     /locus_tag="VVL53_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU4183103.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVESGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14782..16206
                     /gene="gndA"
                     /locus_tag="VVL53_000910"
     CDS             14782..16206
                     /gene="gndA"
                     /locus_tag="VVL53_000910"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU4183104.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16218..16907
                     /locus_tag="VVL53_000911"
     CDS             16218..16907
                     /locus_tag="VVL53_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183105.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17006..18028
                     /gene="cbpC"
                     /locus_tag="VVL53_000912"
     CDS             17006..18028
                     /gene="cbpC"
                     /locus_tag="VVL53_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU4183106.1"
                     /translation="MKLLKKMMQVALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            18046..19044
                     /gene="cbpJ"
                     /locus_tag="VVL53_000913"
     CDS             18046..19044
                     /gene="cbpJ"
                     /locus_tag="VVL53_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU4183107.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(19162..20394)
                     /locus_tag="VVL53_000914"
     CDS             complement(19162..20394)
                     /locus_tag="VVL53_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4183108.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20437..>20811)
                     /locus_tag="VVL53_000915"
     CDS             complement(20437..>20811)
                     /locus_tag="VVL53_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU4183109.1"
                     /translation="LLEKVTSNTENLGNLQLSWCKGISGIILYLCMYDCDGNKDIISK
                     YQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILACSERDDHGLLMFQ
                     GDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         6494 a         3754 c         4720 g         5843 t
ORIGIN      
        1 ttccttgact atgtgatata gttgagggat ttttaaatga tattcatatt ttttgcaaag
       61 atgttgtttg aaaaataatt ttcaaaaatt ctgaaaattc tgttgacaac tttctgaaaa
      121 gagtctataa tggagagaaa gttttaaagg agaaaatgat gaaaagttca aaactacttg
      181 cccttgcggg cgtgacatta ttggcggcga ctactttagc tgcatgctct ggatcaggtt
      241 caagcgctaa aggtgagaag acattctcat acatttatga gacagaccct gataacctca
      301 actatttgac aactgctaag gctgcgacag caaatattac cagtaacgtg gttgatggtt
      361 tgctagaaaa tgatcgctac gggaactttg tgccgtctat ggctgaggat tggtctgtat
      421 ccaaggatgg attgacttac acttatacta tccgtaagga tgcaaaatgg tatacttctg
      481 aaggtgaaga atacgcggca gtcaaagctc aagactttgt aacaggacta aaatatgctg
      541 ctgataaaaa atcagatgct ctttaccttg ttcaagaatc aatcaaaggg ttggatgcct
      601 atgtaaaagg ggaaatcaaa gatttctcac aagtaggaat taaggctctg gatgaacaga
      661 cagttcagta cactttgaac aaaccagaaa gcttctggaa ttctaagaca accatgggtg
      721 tgcttgcgcc agttaatgaa gagtttttga attcaaaagg agatgatttt gccaaagcta
      781 cggatccaag tagtctcttg tataacggtc cttatttgtt gaaatccatt gtgaccaaat
      841 cctctgttga atttgcgaaa aatccgaact actgggataa ggacaatgtg catattgaca
      901 aagttaaatt gtcattctgg gatggtcaag ataccagcaa acctgcagaa aactttaaag
      961 atggtagcct tacagcagct cgtctctatc caacaagtgc aagtttcgca gagcttgaga
     1021 agagtatgaa ggacaatatt gtctatactc aacaagactc tattacgtat ctagttggta
     1081 caaatattga ccgtcagtcc tataaataca catctaagac cagcgacgaa caaaaggcat
     1141 cgactaaaaa ggctctctta aacaaggatt tccgtcaggc tattgcattt ggatttgacc
     1201 gtacagccta tgcctctcag ttgaatggac aaactggagc aagtaaaatc ttgcgtaatc
     1261 tctttgtgcc accaacattt gttcaagcag atggtaaaaa ctttggcgat atggtcaaag
     1321 agaaattggt cacttatggg gatgaatgga aggatgttaa tcttgcagat tctcaggatg
     1381 gtctttacaa tccagaaaaa gccaaggctg aatttgctaa agctaaatca gccttacaag
     1441 cagaaggtgt gacattccca attcatttgg atatgccagt tgaccaaaca gcaactacaa
     1501 aagttcagcg cgtccaatct atgaaacaat ccttggaagc aactttagga gctgataatg
     1561 tcattattga tattcaacaa ctacaaaaag acgaagtaaa caatattaca tattttgctg
     1621 aaaatgctgc tggcgaagac tgggatttat cagataatgt cggttggggt ccagactttg
     1681 ccgatccatc aacctacctt gatattatca aaccttctgt aggagaaagt actaaaacat
     1741 atttagggtt tgactcaggg gaagataatg tagctgctaa aaaagtaggt ctatatgact
     1801 acgaaaaatt ggttactgag gctggtgatg aggctacaga tgttgctaaa cgctatgata
     1861 aatacgctgc agcccaagct tggttgacag atagtgcttt gattattcca actacatctc
     1921 gtacagggcg tccaatcttg tctaagatgg taccatttac aataccattt gcattgtcag
     1981 gaaataaagg tacaagtgaa ccagtcttat ataaatactt ggaacttcaa gacaaggcag
     2041 tcactgtaga tgaataccaa aaagctcagg aaaaatggat gaaagaaaaa gaagagtcta
     2101 ataaaaaggc tcaagaagat ctcgcaaaac atgtgaaata actgttgcaa aatataagaa
     2161 aggatttagt atttctcttg aatgctgaat ccttttttac atttgtaaag aaagattcta
     2221 aatgtattga cccccaaaag ttggagcctc tttttgtcag aatagagaaa atttttgtta
     2281 attttacttg tttcctattg ctttctcagc tattatttgt tatattaaaa gtataattat
     2341 tttttattta tcagggttaa gcattgcact ttcagaggaa ggagtatttt ttaaaaagga
     2401 aatgtaaacg cttgctcaaa aatgaaagga tttagaagtt tatgaataaa ggattatttg
     2461 aaaaacgttg taaatatagt attcggaaat tttcattagg tgttgcttct gttatgattg
     2521 gagctgcatt ctttgggaca agtccggttc ttgcagatag cgtgcagtct ggttccacgg
     2581 cgaacttacc agctgattta gctactgctc ttgcaacagc aaaagagaat gatgggcgtg
     2641 attttgaagc gcctaaggtg ggagaagacc aaggttctcc agaagttaca gatggaccta
     2701 agacagaaga agaactatta gcacttgaaa aagaaaaacc ggctgaagaa aaaccaaaag
     2761 aggataaacc tgcagctgct aaacctgaaa cacctaagac ggtaacccct gaatggcaaa
     2821 cggtagagaa aaaagaacaa cagggaacag tcactatccg agaagaaaaa ggtgtccgct
     2881 acaaccaatt atcctcaact gctcaaaatg ataacgcagg taaaccagcc ctgtttgaaa
     2941 agaagggctt gaccgttgat gccaatggaa atgcaactgt tgatttaacc ttcaaagatg
     3001 attctgaaaa gggcaaatca cgctttggtg tcttcttgaa atttaaagat accaataata
     3061 atgtttttgt cggttatgac aaggatggct ggttctggga gtataaatct ccaacagcta
     3121 gcacttggta tagaggtagt cgtgttgctg ctcctgaaac aggatcaaca aaccgtctct
     3181 ctatcactct caagtcagac ggtcagctaa atgccagcaa taacgatgtc aatctctttg
     3241 acacagtgac tctaccagct gcggtcaatg accatcttaa aaatgagaag aagattcttc
     3301 tcaaggcggg ctcttatgac gatgagcgaa cagttgttag cgttaaaacg gataaccaag
     3361 agggggtaaa aacagaggat acccctgctg aaaaagaaac aggtcctgaa gttgatgata
     3421 gcaaggtgac ttatgacacg attcagtcta aggttctcaa agcagtgatt gaccaagcct
     3481 tccctcgtgt caaggaatac agcttgaatg gacatacttt gccaggacag gttcaacagt
     3541 tcaaccaagt ctttatcaat aaccaccgaa tcacccctga agtcacttat aagaaaatca
     3601 atgagacaac agcagagtac ttgatgaagc ttcgcgatga tgctcactta atcaatgcgg
     3661 aaatgacagt acgcttgcaa gttgtggaca atcaattgca ctttgatgtg accaagattg
     3721 tcaaccacaa tcaagtcact ccaggtcaaa agattgatga cgaaagaaaa ctactttctt
     3781 ctattagttt cctcggcaat gctttagtct ctgtttctag tgatcaaact ggtgctaagt
     3841 ttgatggggc aaccatgtca aacaatacgc atgtcagcgg agatgatcat atcgatgtaa
     3901 ccaatccaat gaaagaccta gccaagggtt acatgtatgg atttgtttct acagataagc
     3961 ttgctgctgg tgtttggagt aactctcaaa acagctatgg tggtggttcg aatgactgga
     4021 ctcgtttgac agcctataaa gaaacagtcg gaaatgccaa ctatgtagga atccacagct
     4081 ctgaatggca atgggaaaaa gcttataagg gcattgtttt cccagaatac acgaaggaac
     4141 ttccaagtgc taaggttgtt atcactgaag atgccaatgc agacaagaaa gtcgattggc
     4201 aggatggtgc cattgcttat cgtagcatta tgaacaatcc tcaaggttgg gaaaaagtta
     4261 aggatatcac agcttaccgt atcgcgatga actttggttc tcaagcacaa aacccattcc
     4321 ttatgacctt ggatggtatc aagaaaatca atctccacac agatggtctt gggcaaggtg
     4381 ttctccttaa aggatatggt agcgaaggcc atgactctgg tcacttgaac tatgctgata
     4441 ttggtaagcg tatcggtggt gtcgaagact tcaagaccct aattgagaag gctaagaaat
     4501 atggagctca tctaggtatc cacgttaacg cttcagaaac ttatcctgag tctaaatact
     4561 tcaatgaaaa aattctccgt aagaatccag atggaagcta tagctatggt tggaactggc
     4621 tagatcaagg tatcaacatt gatgctgcct atgacctagc tcatggtcgt ttggcacgtt
     4681 gggaagattt gaagaaaaaa cttggtgacg gtctcgactt tatctatgtg gacgtttggg
     4741 gtaatggtca atcaggtgat aacggtgcct gggctaccca cgttcttgct aaagaaatta
     4801 acaaacaagg ctggcgcttt gcgatcgagt ggggccatgg tggtgagtac gactctacct
     4861 tccatcactg ggcagctgac ttgacctacg gtggctacac caataaaggt atcaacagtg
     4921 ccatcacccg ctttatacgt aaccaccaaa aagatgcttg ggtaggggac tacagaagtt
     4981 atggtggtgc agccaactat ccactgctag gtggctacag catgaaagac tttgaaggct
     5041 ggcaaggaag aagtgactac aatggctatg taactaactt atttgcccat gacgtcatga
     5101 ccaagtactt ccaacacttc actgtaagta aatgggaaaa tggtacaccg gtgactatga
     5161 ccgataacgg tagcacctat aaatggactc cagaaatgcg agtggaattg gtagatgctg
     5221 acaataataa agtagttgta actcgtaagt caaatgatgt caatagtcca caatatcgcg
     5281 aacgtacagt aactctcaac ggacgtgtca tccaagatgg ttcagcttac ttgactcctt
     5341 ggaactggga tgcaaatggt aagaaacttt ctactgataa ggaaaagatg tactacttca
     5401 atacgcaggc cggtgcaaca acttggaccc ttccaagcga ttgggcaaag agcaaggttt
     5461 acctttacaa gctaactgac caaggtaaga cagaagagca agaactaact gtaaaagatg
     5521 gtaaaattac cctagatctt ctagcaaatc aaccatacgt tctctatcgt tcgaaacaaa
     5581 ccaatcctga aatgtcatgg agtgaaggca tgcacatcta tgaccaagga tttaacagtg
     5641 gtaccttgaa acattggacc atttcaggcg atgcttctaa ggcagaaatt gtcaagtctc
     5701 aaggggcaaa cgatatgctt cgtattcaag gaaacaaaga aaaagttagt ctcactcaga
     5761 aattaactgg cttgaaacca aataccaagt atgccgttta tgtcggtgtc gataaccgta
     5821 gtaatgccaa ggcgagcatc actgtaaata ctggtgaaaa agaagtgact acttatacca
     5881 ataagtctct cgccctcaac tatgtaaaag cctatgccca caatacacgt cgtaacaatg
     5941 ctacagttga cgatacaagt tacttccaaa acatgtacgc cttctttaca actggatcgg
     6001 acgtatcaaa tgttactctg acattgagtc gtgaagctgg tgatgaagca acttactttg
     6061 atgaaattcg tacctttgaa aacaattcaa gcatgtacgg agacaagcat gatacaggta
     6121 aaggcacctt caagcaagac tttgaaaatg ttgctcaggg tatcttccca tttgtagtgg
     6181 gtggtgtcga aggtgtcgaa gacaaccgca ctcacttgtc tgaaaaacac gatccatata
     6241 cacaacgtgg ttggaatggt aagaaagtcg atgatgttat cgaaggaaat tggtcactca
     6301 agacaaatgg actagtgagc cgtcgtaact tggtttacca aaccatccca caaaacttcc
     6361 gttttgaagc aggtaagacc taccgtgtaa cctttgaata cgaagcaggt tcagacaata
     6421 cctatgcttt tgtagtcggt aagggagaat tccagtcagg tcgtcgtggt actcaagcaa
     6481 gcaacttgga aatgcatgaa ttgccaaata cttggacaga ttctaagaaa gccaagaagg
     6541 caaccttcct cgtgacaggt gcagaaacag gggatacttg ggtaggtatc tactcaactg
     6601 gaaatgcaag taatactcgt ggtgattctg gtggaaatgc caacttccgt ggttataacg
     6661 acttcatgat ggataatctt caaatcgaag aaattaccct aacaggtaag atgttgacag
     6721 aaaatgctct gaagaactac ttgccaacgg ttgccatgac taactacacc aaagagtcta
     6781 tggaggcttt gaaagaggcg gtctttaacc tcagtcaggc cgatgatgat atcagtgtgg
     6841 aagaagcgcg tgcagagatt gccaagattg aagccttgaa gaatgctttg gttcagaaga
     6901 aaacggcttt ggtagcagat gactttgcaa gtcttacagc tcctgctcag gctcaagaag
     6961 gtcttgcaaa tgcctttgat ggaaacttat ctagtttatg gcatacatca tggggcggag
     7021 gagatgtagg caagcctgca accatggtct tgaaagaacc aactgaaata actggacttc
     7081 gttatgttcc acgtggatca ggttcaaatg gtaacttgcg tgatgtgaaa cttgttgtga
     7141 cagatgagtc tggcaaggag cataccttta ctgcaactga ttggccagat aacaataagc
     7201 caaaagacat tgactttggt aagacaatta aggctaagaa aattgtcctt acaggtacta
     7261 agacttacgg agatggtggc gataaatacc aatctgcagc ggaacttatc tttactcgtc
     7321 cacaggtagc agaaacacct cttgacttgt caggctatga agcagctttg gctaaggctc
     7381 agaaattaac agacaaagac aatcaagagg aagtagctag cgttcagtca agcatgaaat
     7441 atgcgacgga taaccatctc ttgacggaaa gaatggtgga atactttgca gattatctca
     7501 accaattaaa agattctgct acgaaaccag atgctccaac tgtagagaaa cctgagttta
     7561 aacttagctc tttagcttcc gatcaaggta agatgccaga ttataagcaa gaaatagcta
     7621 gaccagaaac acctaaacaa atcttgccag caacaggtga gagtcaatct gacacagccc
     7681 tcttcctagc aggtgttagc ctagccctat ctgctctctt tgtagtaaaa acgaagaaag
     7741 actagtattt agtaaaacct cttaacaaga ttacggaagc agtctctatc ttttccaatg
     7801 aggtttatag tacagaaaaa gcctgagaag atgtcttctc aggcttttgt taagcacata
     7861 aatacaatag tgctatgaaa aaatcaccca gaaaaatctg ggtgataaat gttatggttg
     7921 tgctggttga ggattctgat tttgttgatc aggggttgta tttgattgtt gcgtattatt
     7981 gttaggattg gtagtcgtac tattatttgt gcttggagtg gttgagctag actgtgaagt
     8041 tgaactatct gatgatgagc ttgaactttc agttgatggg ggttgttgtg gagcaggtga
     8101 gttccacgta gaacgagcac catttttaaa tacgaattct ccatttctgt agagcccctc
     8161 tggtatattc caatcttctg gattgcttcc ttcagacagg taggtcatca tagagcggta
     8221 aactttagca gcgaccgtaa ggccatcgcc tacaagtggt gtcagacggt tagaatagcc
     8281 tgtccataca gccattgcat atttgcgggt ataccctaca aacatttcat ctggagctac
     8341 gtaaccagtg ttcttgatat acttttcaat ttcttcgtca gtatagttag aagtacctgt
     8401 tttacctgct tgtggaagcc aaggtaggta ggctccacgt ccgattccgt atactaagac
     8461 agttttcatc atgtcggtca tcatataggc tgtcgtttcc ttcatggcac gagttccgac
     8521 attagagaac tctttttcac tcccatcact aaagacgact ttatggatat acattggttt
     8581 atagtaagtt ccaccatttg caaaggcagc gtaagcagca gccatctttt cactacttgc
     8641 tccatatttt ttgtctgact cggttgtgtt acttgaaatg gcatttgagt agtgaagact
     8701 tgggtagtcg attccgagac catttaggaa agtcttggca cggttgagtc cgaccttgtt
     8761 tagcgtttcc acggctggga cgtttcgcga ttgttgcagg gcgtattgca aggtgatatt
     8821 gccaaagtag cccctatccc agttataaac aggggtatct gtcccaggat agttataggg
     8881 ctcatcgtga acgatagtag cagttgaatc gtagacaccg tattccaagg caggagcata
     8941 gtctgtgatc ggtttcatag ttgatcccca gtcgcggttt gtttccactg cttggttaat
     9001 tccgaaggaa acattacttg actgatggcg tgctcctagc tgggcaatga ctttaccgtt
     9061 agaaacatca acaatggtag aagcgacttg caattcatcg tctggatagg caacgtattc
     9121 gtctgtattg taaatatccc acagatgttt ttgagcttct tggtctacat ttgtgtagac
     9181 atccatccca gttgtgagca ggttatatcc tgtttcttct tcaacttgat tgatgacttc
     9241 cttgaggtaa ttatccatgt aagcagggta attacttgct gatttgagac tttgtagtcc
     9301 atcagtaatt ggtgtattga ctgctttctc atactgttca gcagagatgt aaccttgatt
     9361 tttcatttca gataagacca agtttcggcg gtcttgggct gcttctggat gtgaataggg
     9421 gtcatattgg tttggtgcct gaggcattcc agccagcaag gctaactgag gtaaacttaa
     9481 attattgagg tctttaccat agtagttttg agctgctgtc tgcattccat agttgccatt
     9541 agacatgtag accttattta tatagtaggt caagatttct tgcttggttg ctttttgttc
     9601 taactgaatc gctaaccaag cttcctgagc cttacgagaa atagtctggt cggaagtcga
     9661 ggttgaaaag taagtcaact taatcaactg ttgggtgaga gttgatccac cttggaggga
     9721 attgctttgc agattgcgca agaaagctcc caggatacgg atggtatcaa tccccctgtg
     9781 gtcgaagaag cgatggtctt cgatagaaac gattgcctta accaaatctg tgggaatatc
     9841 attagcttgg gcattgacgc ggcgttcaga acccaagtca gcaatgagtt gatttttatt
     9901 gtcgtagatt ttactagaag ttgttgcaac tagtttactc tcggataggc taggagcctt
     9961 gctaacgtag tagaaaaaaa ctcctccgcc taagacaatg gctgcgataa ccaagcttaa
    10021 gaagctaatg ctcagatact tgattaggcg cagaatcgtt ggtttgttca tcttgtttta
    10081 ccacctaata aatgttcttt gataacattg agataaggaa tttgagggaa ggcaccagcc
    10141 ttgatttcat atccatattc tcgaatatat tcaagtggca ttgatttttg tcccttatct
    10201 tgatgataga agcgaatcaa atcgaatgcc ggcaataagt aggtttcttg ctgagaagaa
    10261 aagtgaagaa ggacaaagca gattccttgt tgggcaagga cttgttccat atgctgaatc
    10321 tgatgtggat gaaaattttt catcggaatc gcacgttttt gttttgtttc cttgacttca
    10381 aagtcgatgt aatatccatt ataaacgcca gaatagtccg tcgttgaagc ttgtcgaaaa
    10441 taggcttcaa caatcttggc acgacttcgt tgtggatagt ccacttgtac gatttgaata
    10501 ggagttggtt tcttatgtat aacagccaag ccctgagaca aatagtagtc gttggtagca
    10561 ttgatcatct tttcaaaaga cattcctcga tttgcgaaat ttttgggttg agaaagagat
    10621 gtttgtcttt tttgtgatga aactttatgt ggatagttga ccataattct ccttattggt
    10681 acaataacat cactttatta taccataatt tgcacagaaa gggttaaaaa tggctacagc
    10741 tttggtttta ggctattctg cttttgattt gggtttattt tctgataagg atcctcgtct
    10801 taaattgatt aaaaaagcga tacgtaaaga tttagaggct atggcagcag atggggtgtc
    10861 ttggcttgtg tttacaggga gtttgggttt tgaatattgg gttttagagg ttgctcagga
    10921 gatgaaaact gaatacggtt tccagttggc caccattttt gcttttgaaa cccatgggga
    10981 aaattggaat gaaggcaatc agatgaaact gagtcgcttt aagcaggtag actttgtcaa
    11041 atatgcctat cctcgctatg aacacaaggg gcagttaaga gattaccagc agtttttgct
    11101 ggaaaacacg actagttcct atctttttta tgatgaagaa aatgaaacaa aactagctta
    11161 tttttaccaa aagatgaaaa atcaagagga ctattttata aagagattaa catttgatga
    11221 attaaatgaa cttgctgaaa atttttccga aaattgaagc tttgaccttg atttttgttt
    11281 gccttttttt atataataat actagcaagc gagaatggag agagacatgg caagtattat
    11341 tttttcagcg aaagatattt ttgaacaaga gtttggacgt gaggtccgtg gctataataa
    11401 agtagaagtt gacgagtttt tagacgatgt catcaaggac tatgaaacct atgctgcctt
    11461 ggtcaagtca cttcgtcagg aaattgcgga tttgaaggaa gaattaactc gtaaaccgaa
    11521 accttcacca gttcaagcag aaccccttga agcggcaatt acaagttcta tgacgaattt
    11581 tgatattttg aaacgcctga atagattgga aaaagaagtt tttggtaaac aaattttaga
    11641 taactcagat ttttaagtag ttatttgaga tgtgcaattt ttggataatc gcgtgaggag
    11701 aattgtttct catgaggaaa gtccatgcta gcacaggctg tgatgcctgt agtgtttgtg
    11761 ctaggcgaaa ccataagcct agggacgaga aatcgttacg gcagttgaaa tggctaagtc
    11821 cttggatagg ccagagtagg cttgaaagtg ccacagtgac ggagtctttc tggaaacaga
    11881 gagagtggaa cgcggtaaac ccctcaagct agcaacccaa attttggtcg gggcatggag
    11941 tacgcggaaa cgaacgtagt attctgactg ctatcagcta gagctgttag tggtagacag
    12001 atgattatcg aaggaagtgg tcctagtcac ttctggaaca aaacatggct tatagaaaat
    12061 tgcatatagg ttggggctga gaaattttct caacctcatt ttttaaagtg gacatataga
    12121 aaggtcttgc aagactgtaa catgaaaaaa gaatttaatt taattgcaac tgtggcagca
    12181 gggcttgagg ctgtcgttgg tcgtgaagtg cgagagttgg gctacgattg tcaggttgaa
    12241 aatggacgtg ttcgttttca aggagacgtg agagctatta tcgaaaccaa cctttggctt
    12301 cgggcagcag atcgtatcaa aattatcgta ggaacgttcc cagctaagac ttttgaagag
    12361 ctatttcagg gagttttcgc tttggattgg gaaaattatt taccacttgg agctcggttc
    12421 ccgatttcaa aagctaaatg tgttaagtcc aaacttcaca atgagcccag tgttcaggct
    12481 atttctaaga aagctgttgt caagaaattg cagaaacact atgctcgccc agaaggggtt
    12541 cctctgatgg agaatggccc agagtttaag attgaggtct ctattctcaa agatgtggca
    12601 actgtcatga ttgatacgac cgggtctagc ctctttaaac gtggttatcg taccgaaaaa
    12661 ggtggcgctc ctatcaagga aaatatggca gcagccattt tacaactttc taactggtat
    12721 ccagacaagc ctttgattga tccgacctgt ggttcgggga ctttctgtat tgaggcagtt
    12781 atgattgcta gaaagatggc gccaggtctt cgtcgctctt ttgcatttga ggaatggaac
    12841 tggatcagcg atcgcttgat tcaagaagtg cgcacagaag cggctaaaaa agtagaccgt
    12901 gagcttgagc tggatatcat gggctgtgat attgatgctc gcatggtgga aattgctaag
    12961 gccaatgctc aggtagctgg tgttgcagga gacattactt ttaagcagat gcgcgtgcag
    13021 gatttacgtt ccgataaaat caatggagta atcatttcca atccgcctta tggtgaacgt
    13081 ttgtcagatg atgcaggggt gaccaagctc tatgctgaga tggggcaagt atttgcaccg
    13141 ctgaaaactt ggagcaaatt tatcctgact agtgatgaag cttttgaaag caagtatggt
    13201 agccaagcag ataagaagcg taagttatac aacggaacct tgaaagtgga tctatatcaa
    13261 tattttggtc agcgtgtcaa acggcaagag gtaaaataga aagggatact catgagtaaa
    13321 aaaagacgaa atcgtcataa aaaagaaggt caagaaccgc aatttgattt tgatgaagca
    13381 aaagagctaa cagttggtca agctattcgt aaaaatgaag aagtggaatc aggagtcttg
    13441 cctgaggatt ccattttgga caagtatgtt aagcaacaca gagatgaaat tgaggcggat
    13501 aagtttgcga ctcgtcaata caaaaaagag gagttcgttg aaactcagag tctggatgat
    13561 ttaattcaag agatgcgtga ggctgtagag aagtcagaag cttcttcgga ggaagttcca
    13621 tcttctgaag acatcttact acccttgcct ctggacgatg aggagcaagg cttggatcct
    13681 ctattgctag atgatgaaaa tccaacagaa atgactgaag aagtggaaga ggagcaaaac
    13741 ctttctcgtc tggatcaaga ggactcagaa aagaaaagta aaaaaggctt tattttgacc
    13801 gttttggcgc ttgtatcagt aattatttgt gtcagtgctt attatgtcta ccgtcaagtg
    13861 gctcgttcga ctaaggaaat tgaaacttct caatcaacta cagccaatca atcggatgtg
    13921 gatgatttta atacacttta tgacgccttt tacacagata gcaataaaac ggctttgaaa
    13981 aatagccagt ttgataaact gagtcaactc aagactttac ttgataagct agaaggtagt
    14041 cgtgaacata cgcttgccaa atctaaatat gatagtctag caacgcaaat caaggctatt
    14101 caagatgtca atgctcaatt tgagaaacca gctattgtgg atggtgtgtt ggataccaat
    14161 gccaaagcca aatcggatgc taaatttacg gatattaaaa ctggaaatac ggagcttgat
    14221 aaagtgctag ataaggctat cagtcttggt aagagccagc aaacaagtac ttctagctca
    14281 agttcaagtc aaactagcag ctcaagttca agtcaagcaa gttcaaatac gactagtgag
    14341 ccaaaaccaa gtagttcaaa tgagactaga agtagtcgca gtgaagtcaa tatgggtctc
    14401 tcgagtgcag gggttgctgt tcaaagaagt gccagtcgtg ttgcctataa tcagtctgct
    14461 attgatgata gtaataactc tgcctgggat tttgcggatg gtgtcttgga acaaattcta
    14521 gcgacttcac gttcacgtgg ctatatcact ggagaccaat atatccttga acgtgtcaat
    14581 atcgttaacg gcaatggtta ttacaacctc tacaagccag atggaaccta tctctttacc
    14641 cttaactgta agacaggcta ctttgtcgga aatggcgctg gtcatgcgga tgacttagat
    14701 tactaagcag tcgttacaaa attctttctt ttcaaaagta aaaatgataa aataaaacaa
    14761 atgaaacaag aggagtgtca aatgacaaaa gctaactttg gtgtcgtagg tatggccgta
    14821 atgggtcgta accttgccct taatattgaa tctcgtggtt acacagttgc tatctacaac
    14881 cgtagtaaag aaaaaacgga agatgtgatt gcttgccatc ctgaaaagaa ctttgtacca
    14941 agctatgacg ttgaaagttt tgtaaactca atcgaaaaac ctcgtcgtat catgctgatg
    15001 gttcaagctg gacctggtac agatgctact atccaagccc ttcttccaca ccttgacaag
    15061 ggtgatatct tgattgacgg aggaaatact ttctacaaag ataccatccg tcgtaatgaa
    15121 gaattggcaa actctggtat caactttatc ggtactgggg tttctggtgg tgaaaaaggt
    15181 gcccttgaag gtccttctat catgcctggt ggacaaaaag aagcctacga attggttgcg
    15241 gatgttcttg aagaaatctc agctaaagca ccagaagatg gcaaaccatg tgtgacttac
    15301 atcggtcctg atggagctgg tcactatgtg aaaatggttc acaatggtat tgagtacggt
    15361 gatatgcaat tgatcgcaga aagctatgac ttgatgcaac acttgctagg cctttctgca
    15421 gaagatatgg ctgaaatctt tactgagtgg aacaagggtg aattagacag ctacttgatt
    15481 gaaatcacag ctgatatctt gagccgtaaa gacgatgaag gccaagatgg accaatcgta
    15541 gactacatcc ttgatgctgc aggtaacaag ggaactggta aatggactag ccaatcatct
    15601 cttgaccttg gtgtaccatt gtcactgatt actgagtcag tgtttgcacg ctacatttca
    15661 acttacaaag aagaacgtgt acatgctagc aaggtgcttc caaaaccagc tgccttcaac
    15721 tttgaaggag acaaggctga attgattgaa aagatccgtc aagcccttta cttctcaaaa
    15781 atcatttcat acgcacaagg atttgctcaa ttgcgtgtag cctctaaaga aaacaactgg
    15841 aacttgccat ttgcagatat cgcatctatc tggcgtgatg gctgtatcat ccgttctcgt
    15901 ttcttgcaaa agattacaga tgcttacaac cgcgatgcag atcttgccaa ccttcttttg
    15961 gacgagtact tcttggatgt tactgctaag taccaacaag cagtacgtga tatcgtagct
    16021 cttgcggttc aagcaggtgt gccagtgcca actttctcag cagctattac ttactttgat
    16081 agctaccgtt cagctgacct tccagctaac ttgatccaag cacaacgtga ctactttggt
    16141 gctcacactt accaacgtaa agacaaagaa ggaaccttcc actactcttg gtatgacgaa
    16201 aaataagtag gtcagccatg gggaaacgga ttttattact tgagaaagaa cgaaatctag
    16261 ctcatttttt aagtttggaa ctccagaaag agcagtatcg ggttgatctg gtagaggagg
    16321 ggcaaaaagc cctctccatg gctcttcaga cagactatga tttgatgtta ttgaacgtta
    16381 atctgggaga tatgatggct caggattttg cagaaaaatt gagccgaact aaacctgcct
    16441 cagtcatcat gattttagat cattgggaag acttgcaaga agagctggaa gttgttcagc
    16501 gttttgcagt ttcatacatc tataagccag tccttatcga aaatctggta gcgcgtattt
    16561 cggcgatctt ccgaggtcgg gacttcattg atcaacactg cagtctgatg aaagttccaa
    16621 ggacctaccg caatcttagg atagatgttg aacatcacac ggtttatcgt ggtgaagaga
    16681 tgattgctct gacacgccgt gagtatgacc ttttggcgac acttatggga agcaagaaag
    16741 tattgactcg tgagcaattg ttggaaagtg tttggaagta tgaaagtgcg accgagacaa
    16801 atatcgtaga tgtctatatc cgctatctac ggagcaagct tgatgttaaa ggccaaaaaa
    16861 gctacattaa aactgtgcgt ggtgttggat ataccatgca agaatagaaa agcagttgca
    16921 gttgtgtaac tgcttttttg aggagttttt atatattgac atacaatcta gtttttacta
    16981 caatcgtttt aggaggatat ataaaatgaa gcttttgaaa aaaatgatgc aagtcgcatt
    17041 agcagtcttt ttctttggtt tgctagctac aaatacggta tttgcgaata ccacaggtgg
    17101 ccgatttgtt gataaggata atagaaaata ttatgtaaaa gatgatcata aagcaatcta
    17161 ttggcataaa atagacggta aaacttacta ttttggtgat attggagaga tggttgtcgg
    17221 ttggcaatac ttagaaattc ctggaacagg ttatcgtgat aatttattcg ataaccaacc
    17281 agttaatgaa attggccttc aggagaagtg gtactatttt ggacaagatg gtgctttgct
    17341 agaacaaaca gataaacaag tactagaggc aaaaacgtct gaaaatacag gaaaagtata
    17401 cggtgaacaa tatcctctat ctgctgaaaa gagaacttat tattttgata ataattatgc
    17461 tgtaaagaca ggctggattt atgaagaggg tcattggtat tatttaaata agctaggaaa
    17521 ttttggcgat gattcttaca atccactacc aattggtgaa gttgctaagg gttggactca
    17581 agattttcat gttactattg acattgatag aagcaaacct gctccatggt actacctaga
    17641 tgcttcaggt aagatgctta cagattggca aaaagtaaac ggaaaatggt attattttgg
    17701 ctcctctggt tctatggcaa caggttggaa atatgtacga ggcaaatggt attacttaga
    17761 taataaaaat ggtgatatga aaacaggatg gcaatacctt ggtaacaagt ggtactacct
    17821 ccgttcatca ggagctatgg taactggctg gtatcaagat ggttcaactt ggtactattt
    17881 agatccttct aatggagata tgaaaatagg ttggacaaaa gtaaatggaa aatggtatta
    17941 tctcaattca aatggagcaa tggttacagg tagccaaact atcgatggta aagtttataa
    18001 tttcgcctca tctggtgagt ggatttaata ttggagggta tatagatgaa aattttgaaa
    18061 aaaactatgc aagttggact gacagtattt ttctttggtt tgctagggac cagtacagta
    18121 tttgcagatg attctgaagg atggcagttt gtccaagaaa acggaagaac ctactacaaa
    18181 aagggggacc tcaaagaaac ctactggcga gtgattgatg gtaagtacta ttattttgat
    18241 tctctatctg gagagatggt tgtcggctgg caatatatcc cgtttccatc taaaggtagt
    18301 acaattggtc cttacccaaa tggtatcaga ttagaaggtt ttccaaagtc agagtggtac
    18361 tacttcgata aaaatggagt gctacaagag tttgttggtt ggaaaacatt agagattaaa
    18421 actaaagaca gtgttggaag aaagtacggg gaaaaacgtg aagattcaga agataaagaa
    18481 gagaagcgtt attatacgaa ctattacttt aatcaaaatc attctttaga gacaggttgg
    18541 ctttatgatc agtctaactg gtattatcta gctaagacgg aaattaatgg agaaaactac
    18601 cttggtggtg aaagacgtgc ggggtggata aacgatgatt cgacttggta ctacctagat
    18661 ccaacaactg gtattatgca aacaggttgg caatatctag gtaataagtg gtactacctc
    18721 cgttcatcag gagcaatggc cactggctgg tatcaggaag gtaccacttg gtattattta
    18781 gaccacccaa atggcgatat gaaaacaggt tggcaaaacc ttgggaacaa atggtactat
    18841 ctccgttcat caggagctat ggcaactggt tggtatcaag atggttcaac ttggtactac
    18901 ctaaatgcag gtaatggaga catgaagaca ggttggttcc aggtcaatgg caactggtac
    18961 tatgcttata gctcaggtgc tttggcagtg aatacgaccg tagatggcta ttctgtcaac
    19021 tataatggcg aatgggttcg gtaatgacag ggcgcttata attatattag aacactcgct
    19081 taatcaaaga agtgactttg ttgtacttga cctgacgatt ggggagatta aaagcatgtg
    19141 aaatctacac atgctttttc gttagttatg agtgttgaac ttgactaacc attgacaaaa
    19201 gataagagca attaaaataa gatacaaaaa tatcaatgct aactgcaccg aaacgcccga
    19261 tgctatggat agggctaata gggagagcag ggacggggcg agaatgtcca ctgtgctcag
    19321 agcagagccc accgtcccca tcttgtcact ggggatatgg gcaaagacag ctgcttgtag
    19381 gcgagggctg agaatgccga taatcgtaga actgatgaaa ctggtcatga aaattacgat
    19441 ataggcttga caaaggatac tagttattag agtcaatagg gaaatctcac agaaaaccaa
    19501 gaggtgcgtg aaacggatat ttttaaatag attactgctg gtcatattgc caaggatagc
    19561 tccacaagac atgatgatgg agagcagggc gaggagttgc ccaatgctca gttgtgctag
    19621 tgtctgttgg atgaagcgga gggcaatcag ccgaggggac acatccaaga tggcaatcat
    19681 actggtcgtc agaaacagta gtatgacggt atcttttaac ctcaggagca cagccagcga
    19741 ctgtttaaga tgttgaaaat atttcttaaa actcagtgct gtatgtgaca tttcaattct
    19801 tttttcaacc tcatacaagg tatgtcgaat aacatacagg cccaaaaagg caatgacaaa
    19861 agtcagagtg ttgataaggg aaatagtttg aatacttata acattgataa ggaatgcgcc
    19921 agccagattg gcgacaatac ggacaaccct catcagagac tgcctgaacc ccatggcatc
    19981 atgcaggtcg tccttaatta cactgatgta gagggcgttc atcatgtagg cagacaggta
    20041 gctcaaggtg tcagagatga ggttgatgat gacaatcata atgactgaaa ctaatgaaaa
    20101 gggcgacaag acgagaaata ctgtcaaaat agcgtagagc aggattttga ttttggcaat
    20161 ccagagtgcg tgtttgactc tattttcttg aaaatctcct agaatcccga gaaagagttg
    20221 cgatagtagg ggaaccatct ctgaaatgga aatcagtaaa atcgctagcg gagcgaaatt
    20281 gtactgagcc acataattga taatggcgag gtagaaaata ctgtcgccga atgatgaaaa
    20341 acagctgttt agcagcaact gtctatatat gttgtttgtc caaaataatt tcatactttt
    20401 ctccttatgt ctgcacatcg aatgggaatt tattatttaa tagacaccaa tataccccat
    20461 gcttcctatt ccgaagtcaa acaaatctgc tttaccacta tctccttgaa acatcagtaa
    20521 accgtgatca tctcgttcag aacatgctaa aattacctgt tggatttttt tcatcagtaa
    20581 tttgttttga ttgtagacag tggtttgtag taagctagtt attccgtggc aatatcctgt
    20641 catcattttt agatgatggt taaaaacaaa ttcttgatat ttactaataa tatctttgtt
    20701 tccgtcacaa tcgtacatac aaagatataa gattattccg gaaattcctt tgcaccaaga
    20761 aagttgtaaa tttcctaaat tttcagtatt agaagtaact ttttctaata a
//
LOCUS       DAVHEW010000027        20497 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVHEW010000027 DAVHEW010000000
VERSION     DAVHEW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.027"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            305..1168
                     /locus_tag="VVL53_000916"
     CDS             305..1168
                     /locus_tag="VVL53_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU4183110.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKLLWQFLKKMSIFLQTKGSNQKEIENFIQSLQVLENPQLTALFE
                     LRFQQYKELID"
     gene            1459..1692
                     /locus_tag="VVL53_000917"
     CDS             1459..1692
                     /locus_tag="VVL53_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183111.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            1714..2391
                     /locus_tag="VVL53_000918"
     CDS             1714..2391
                     /locus_tag="VVL53_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183112.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            2403..3068
                     /locus_tag="VVL53_000919"
     CDS             2403..3068
                     /locus_tag="VVL53_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183113.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3140..4366
                     /locus_tag="VVL53_000920"
     CDS             3140..4366
                     /locus_tag="VVL53_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4183114.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4763..4840)
                     /locus_tag="VVL53_000921"
                     /pseudo
     CDS             complement(4763..4840)
                     /locus_tag="VVL53_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4911..5567
                     /locus_tag="VVL53_000922"
     CDS             4911..5567
                     /locus_tag="VVL53_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU4183115.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            5557..5880
                     /locus_tag="VVL53_000923"
     CDS             5557..5880
                     /locus_tag="VVL53_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU4183116.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            5984..6814
                     /locus_tag="VVL53_000924"
     CDS             5984..6814
                     /locus_tag="VVL53_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4183117.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            6968..7822
                     /locus_tag="VVL53_000925"
     CDS             6968..7822
                     /locus_tag="VVL53_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4183118.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            7922..9295
                     /locus_tag="VVL53_000926"
     CDS             7922..9295
                     /locus_tag="VVL53_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4183119.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPNAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            9288..10349
                     /locus_tag="VVL53_000927"
     CDS             9288..10349
                     /locus_tag="VVL53_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU4183120.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            10351..11043
                     /locus_tag="VVL53_000928"
     CDS             10351..11043
                     /locus_tag="VVL53_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183121.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11073..11642)
                     /locus_tag="VVL53_000929"
     CDS             complement(11073..11642)
                     /locus_tag="VVL53_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU4183122.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11777..12391
                     /locus_tag="VVL53_000930"
     CDS             11777..12391
                     /locus_tag="VVL53_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU4183123.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12393..14039
                     /locus_tag="VVL53_000931"
     CDS             12393..14039
                     /locus_tag="VVL53_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4183124.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14051..15337
                     /locus_tag="VVL53_000932"
     CDS             14051..15337
                     /locus_tag="VVL53_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183125.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGHPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFDQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKAFFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15389..15922
                     /locus_tag="VVL53_000933"
     CDS             15389..15922
                     /locus_tag="VVL53_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183126.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            16000..16470
                     /gene="nrdI"
                     /locus_tag="VVL53_000934"
     CDS             16000..16470
                     /gene="nrdI"
                     /locus_tag="VVL53_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU4183127.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16763..18025)
                     /locus_tag="VVL53_000935"
     CDS             complement(16763..18025)
                     /locus_tag="VVL53_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU4183128.1"
                     /translation="MSQAITLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18335..18793)
                     /locus_tag="VVL53_000936"
     CDS             complement(18335..18793)
                     /locus_tag="VVL53_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU4183129.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(18790..19230)
                     /locus_tag="VVL53_000937"
     CDS             complement(18790..19230)
                     /locus_tag="VVL53_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183130.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            19663..>20497
                     /locus_tag="VVL53_000938"
     CDS             19663..>20497
                     /locus_tag="VVL53_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183131.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LK"
BASE COUNT         5979 a         3718 c         4515 g         6285 t
ORIGIN      
        1 taggttcttt gtaaccgctc cattataaaa tcctttcact cttccggtat actattagga
       61 aaaacataac tggaggatat tttgagctca cccctcatcg ttccagtggc tgttccccaa
      121 gctcgtgtac tgcccaaaaa agttagacat tctatttaga acgaggattg agttctgtat
      181 tgtacaaggc tcggtccttt tagagtcagc ttaaggctgg ctttttcaat caccaaagtg
      241 tcagaatgtt ttgacaaaac tataaaaaca atgatatact atataaaaaa taggaggtgg
      301 taggatgatt gaaaaaatgg aattggggga attttacaag gaattacgtt tggcgagaaa
      361 gctcaagcag tcagatgtgg cttgtgacgg gctaacagct tctcagttgt ccaagtttga
      421 gctagggcag tccatgttgt ctgctgataa gctcatatta gccatccaag ggatcaatgt
      481 gacctttgat gagtttgggc acaagctcaa caactatcaa gaatctccac atatgcagat
      541 tggtagaaag gttgtggatc gctttgctca tcaagatata gctggcttag agcaactgtt
      601 ggaggaagtc gagcaagaac agatggcaga gacttatcgt cgtttgaatg ctattgtaat
      661 caaagatgcc atccactcac tagataaaag ctacccacta gcagaggagg atagcgagtt
      721 tttgaccacc tatctctatg ctatcgagtc ttggacctgg tttgaactct atctcttttg
      781 caatactctg cccttcttga gcaatcaaga tctgatcttt ttatcaacct ccttactcga
      841 aaaatccaaa gaatttaaag agttagtaca caatcgattg tatatgaagc aaggactctt
      901 aaatatcatc tcagagctca tggagcgcaa acttttttct tatatcccaa tctttgaagc
      961 cgagctggag agtatgctcc gtccatacga tgtttttgag aaattattgt ggcagttttt
     1021 aaagaagatg agcattttcc ttcaaaccaa gggaagcaac caaaaagaga ttgaaaattt
     1081 tatccaatct ctgcaagtat tagaaaatcc acaattaaca gccctcttcg aattgcgttt
     1141 tcagcaatat aaagaactta tagattagtt aaaaatggaa tgacaataac ccactccgaa
     1201 ataagtagga gtatcagttg gatttttctt ttcttcatct attatattcc tccttgttag
     1261 taataacttt attataccag ggaaataatc aaatctatca aaatcgcaaa taagaaattt
     1321 ctataagaaa aaatatcaaa tatgcgattt tttaaaataa gccaattttc gtgttatact
     1381 gtacttgtaa agcacttgaa gcaaatccta ggtcgcagaa gtggtttaca aatgaagata
     1441 attgaaggag tgtaaaagat gttaaattta caatttgcag aaacaatgga attgacagaa
     1501 gctgagttgc aagatgttag aggaggcaac ctcgtaaatt ctatgggagg tggtggaaga
     1561 agtggtatat ctggttgggg cgtcccagga atttaccctg gttggggcaa tcaagggatg
     1621 agtcctaatc gtggagcttt tgattggact atagatttag cagatggatt gtttggccgc
     1681 cgtagaagat aggctcttat aggagataat aggatgaaga aaatgaaaga agtgaaattt
     1741 cacctagcaa ctggcttgtt gattttgact tattacctca tttttaatgt cacatcagac
     1801 ttagatttta tggttgcttt gtctgataat atgtactatg tgttccaagt tttattggta
     1861 cttattctgg gaactatagc tacaattgct tttgtcaaaa gtgaacattg gaaagagtgt
     1921 ggtcgtttcc agtttcgttg gtcttacctg ggtgttttcc ttctttcttt tttcttattg
     1981 ttcgtgtggg caaatctaac aacctatatc tttcctcgta cccagaatgg aagtacagtc
     2041 gtagaagtag ccaccaattt aacaggaatt agctattttg ttacgcgaat actttatact
     2101 agtattatcg cacctgtatc agaagaggtt gtttgtcgtg gtcttttgat gacaagttta
     2161 tctaaagtta agagatacta tcttgatgtt cttgtttccg ctgccatatt tggcgctatg
     2221 catgttttgc agtatggttg gataacgaca gatttcataa agtattttgg aatgggcttg
     2281 atattttgta tgatgtttcg ctacacccga tccatctatt gggctatagc tctgcatgct
     2341 tcatggaata gtttcttact cattgttact ttattggtat ttggatatta gaaaggtgat
     2401 atatgaaaaa gataatctca caccgctact ttattatagt ttttctacta gttattgctg
     2461 accagaagtt cagtgttcta gttttacgca gcgaccttgt tactgggcta tctgactttg
     2521 cctattatct gtcggatatg atgttgaatt ttcttgtggt tttatttgct cttattgcta
     2581 tgatttggtc ggggaaatgg cagaaaatca atagtagaaa gtttaaagga tcctatcttt
     2641 tctattcctt cctagctctg cttgcttttg ttgtttggaa tttcgttacc ttttttcttt
     2701 tcccacctac tcgaaatgaa atttcttatc aacatgctgc tccgactttt acaggggcta
     2761 cagcattttt gatgtatttt ttttatcctg tgattgcagg tcccattttt gaagacatga
     2821 tttatcgtgg attagtgatg actgctctgg aaaaaggaaa gaaatggggg ctagatgtac
     2881 ttggttccgc tgttttattt ggggtctcgc acattagtaa tcacggttgg gtcttgacag
     2941 actttgtctt ctatatgggt ggaggtctta tatttgcagt cttatttaga atgacaaagt
     3001 caatttattg gcctattgga ctgcatatag tctacaatgg cattggtcag cttttgatgt
     3061 tactgtaatt ttggttgtta gtagtatcgc ggtctatcct tggggaaaga ttcccatttg
     3121 aacttaagaa ggagttgtga tgaaagtatt tcttcaaaat agagatttta ggcaattaac
     3181 tatcaaccag tggatttcaa cgcttgggga tacgattttt tatctggcct tcctgaatta
     3241 tgtggcagat gcatcttttg cccctttggc gattttactc atcacgattt cagaaaccct
     3301 gccccaagtt ctacaaatct ttctgggagt tttggcggat tttcaacatc atcgtgtctt
     3361 gaaatacaca gtcattagtt ttgcaaaatt tttgctttac tctatagtgt ctttatcact
     3421 ttcagggcag tctttctcct tgttgctagt agcatttatt tgtctgatta acctcttgtc
     3481 tgacacattg agttattttt caggcgccat gctcactccg attttcatta gaattattgg
     3541 gcaagaccat ctggcagaag ctattggctt taaacagtca actgttagtt tagtgaaaac
     3601 aatcagtaat atcctaggag gagtcttact aggcattcta tccatccagt ttatttcctt
     3661 actgaacgct cttacctttt taatcgcatt tttaggtatc ctcttcataa aaactgacct
     3721 cttgaaagta gaaaaaacga ttagctatca agaaggactc tctgtaaaat ccttttgcca
     3781 gcatttgctc caatcatcga aattgatatg gaatatgaat aaggtgctct tggttttgtt
     3841 tattatctct actagtcaag cagtgataaa tgttacggtt cctatttcca ctctattttt
     3901 aaggaaccag ccctttttga atttacaaac tggtcaatct cttgccttgt tagccacact
     3961 agaattatca gccctgattg tcggaagcct tgtaagtggc tatctgcaac ataccatttc
     4021 cataaaaaca gctctctatg cctcacttgt catccagttg cttcttctag taggatttgc
     4081 cacagttcgt tttgactgga ttctcatctt tagtaccttg gatgcctttt tcgcaggtgt
     4141 tctctctcct agattgcaag aactcgtttt taaacaaata cctgaggagt caatgggagc
     4201 agttcaatcc tctatcggtg ccattacggt tgttttacct agcttattta caatagcttt
     4261 ggtaaccatt gctactagct ttggaactct ggcagttagc tttgttttat tgctatttct
     4321 tctagttgcc tttgttatgc ttctgaatat tcgtgaaagt atttagtgga gaatttctat
     4381 aatatatgtt tgagactgga ttgccagtct ttttatgatg gtcaaaatca acagactttt
     4441 ttctcttttc tgaatgtgtt ataatagact gaatctaaaa tagtacgaaa caattgctaa
     4501 aacatttata gaaattaatt ttactttccc aatcgatttg ttctcatctt atttcaatct
     4561 gctatataat agtccatgct gtggctggat tcttttcagc ctacttgtac tcttcgaaac
     4621 tctcttcaaa ccacgtcagc ttcactttgc cgtaggtatg gttactgact tcgtcagttc
     4681 tatccacaac ctcaaaacgg tgttttgagc tgacttcgtc agttctatcc acaacctcaa
     4741 aacagtgttt tgagctgact tcatcagttc tatctgcaac ctcaaaacag tgttttgagc
     4801 aacctgcggc tagtttccta gtttgctctt tgattttcat tgagtattat taagacaata
     4861 tgaggagaaa gatgaaagaa aaaggatttt aggagggggc gcaggcagcc atgccaacgg
     4921 ctcttggcta tgtcagtatc ggcctggcct gtggaattat cggtgcgccc tatgtgacac
     4981 ctgttgagat gggcttgatg agtctctttg tttatgctgg gagtgcccag tttgccatgt
     5041 tggcactgat tgtggttcaa gctcctgtgg cagctattgc tatgacggtt tttctaatca
     5101 acttgcgtct ctttttgttg agtttacacg catcgactta tttccgtcat accagtcttt
     5161 ggtacaatat cggtatgtct agtatcttga cagatgagac ctatggcgtt ttgatgggtg
     5221 aattggccca tacagacaag gtaaatccta tgtggatgca cggaaacaat cttaacagct
     5281 atgtggcttg gtttgtgggg acagtagtcg gaacggctct gggtggcctg ctaccaaatc
     5341 cagaaatctt tggcttggat tttgccctgg ttgggatgtt tattggtatt tttgcttcgc
     5401 aatttcagat tatgcaaaga cggattcctg tccgcaatct gctcattatc ctagcagttg
     5461 ttgcggtgtc cttctttttg ctcttgacag tggtgtctca gtcactagct gttctgtttg
     5521 cgacgctact tggttgtagc atgggggtgg ttttagatgg tcagtaagta tcttttatta
     5581 gcagttattt tctctggctt ggtgacttgg attctccgta tgattccctt catcttagtc
     5641 aagtataagg gcttgcctgc aatcgttgag cgttttttga agttcttgcc cgtttccatt
     5701 atctttgcct tgattctttc aagcgtagtg acaggtaagg ttgggagcct tcctcaaatt
     5761 aaatggctag acttcttagc cgtctttcca acagcttggg tagcctttcg ctaccgcaat
     5821 ctagtcggaa cagttctctt tggagtggtc ttgattgcca tcttgcgttt ggtcttttaa
     5881 attacccata aaaaaaactt atcacagaga tagatatcat ataatggcgt aaatgctctt
     5941 tttctgttaa gattataagg tattctattt tggaggaaat gacatgaaaa aaatcgttaa
     6001 atactcatct cttgctgccc taggacttgt tgctgcaggt gtgcttgcgg cttgctcagg
     6061 gggtgctaag aaagaaggag aagcagctag caagaaagaa atcatcgttg caaccaatgg
     6121 atcaccaagg ccatttatct atgaagaaaa tggcgaattg actggttacg agattgaagt
     6181 cgttcgcgct atctttaaag attctgacaa atatgatgtc aagtttgaaa agacagaatg
     6241 gtcaggtgtc tttgctggtc ttgacgctga ccgttacaat atggctgtca acaatcttag
     6301 ctacactaaa gaacgtgcgg agaaatacct ctatgccgca ccaattgccc aaaatcctaa
     6361 tgtccttgtc gtgaagaaag atgactctag tatcaagtct ctcgatgata tcggtggaaa
     6421 atcgacggaa gtcgttcaag ccactacatc agctaagcag ttagaagcat acaacgctga
     6481 acacacggac aacccaacta tccttaacta tactaaggca gatttccaac aaatcatggt
     6541 acgtttgagc gatggacaat ttgactataa gatttttgat aaaatcggtg ttgaaacagt
     6601 tatcaagaac caaggtttgg acaacttgaa agttatcgaa cttccaagcg accaacaacc
     6661 gtacgtttac ccacttcttg ctcaaggtca agatgagttg aaatcatttg tagacaaacg
     6721 cattaaagaa ctctacaaag acggaacgct tgaaaaattg tctaaacaat tcttcggaga
     6781 tacttatcta ccagcagagg ctgatattaa ataatttctt gaaatcatcc attctgagta
     6841 agatgggtga tttctcagtt tttagggata tagttttaaa ctatatatct gattatctaa
     6901 taacaatttg tgaaatcaaa caaattgtga gatactagta cggtattatt tttaaggaga
     6961 aagaatcatg aaaatcaaaa aatggcttgg cctagtagcc cttgctacag tcgcaggttt
     7021 ggctcttgca gcttgcggaa actcagaaaa gaaagcagac aatgcaacaa ctatcaaaat
     7081 cgcaactgtt aaccgtagcg gttctgaaga aaaacgttgg gacaaaatcc aagaattggt
     7141 taaaaaagac ggaattacct tggaatttac agagttcaca gactactcac aaccaaacaa
     7201 agcaactgct gatggcgaag tagatttgaa cgctttccaa cactataact tcttgaacaa
     7261 ctggaacaaa gaaaacggaa aagaccttgt agcgattgca gatacttaca tctctccaat
     7321 ccgcctttac tcaggtttga atggaagtgc caacaagtac actaaagtag aagacatccc
     7381 agcaaacgga gaaatcgctg taccgaatga cgctacaaac gaaagccgtg cgctttactt
     7441 gcttcaatca gctggcttga ttaaattgga tgtttctgga actgctcttg caacagttgc
     7501 caacatcaaa gaaaatccaa agaacttgaa aatcactgaa ttggacgcta gccaaacagc
     7561 tcgttcattg tcatcagttg acgctgccgt tgtaaacaat accttcgtta cagaagcaaa
     7621 attggactac aagaaagcac ttttcaaaga acaagctgat gaaaactcaa aacaatggta
     7681 caacatcatt gttgcgaaaa aagattggga aacatcacct aaggctgatg ctatcaagaa
     7741 agtaatcgca gcttaccaca cagatgacgt gaaaaaagtt atcgaagaat catcagacgg
     7801 tttggatcaa ccagtttggt aataagaaac agggaggtgg gagagagaat tccacctctt
     7861 gctcttgtat agagcacgga gtgtaaagaa gactattcgt tcatagaaag gtagagagaa
     7921 tatggttttt cctagcgaac aagaacagat tgaaaaattt gaaaaggatc atgtagccca
     7981 gcattatttt gaggttttgc gtaccttgat ttctaagaaa tcagtctttg cccagcaggt
     8041 tggactcaag gaagtcgcaa attatctggg tgagattttc aagcgtgttg gagctgaagt
     8101 ggagattgat gagagctata cagcgccctt tgtcatggca catttcaaga gttcgcgtcc
     8161 aaatgccaag accttgattt tctataacca ctatgacact gtgccagcgg atggggatca
     8221 ggtctggaca gaggatcctt ttacgctttc ggtccgcaat ggcttcatgt atgggcgtgg
     8281 agttgatgac gacaagggtc atatcacagc tcgcttgagt gctttgagaa aatatatgca
     8341 gcaccatgat gatttacctg tcaatatcag ctttatcatg gagggagcgg aggaatcggc
     8401 ttcaacagac ctagataagt atttggaaaa gcatgcagac aaactccgtg gggcggattt
     8461 gttggtctgg gaacaaggga ccaaaaatgc cttggaacag ctggaaattt ctggtggcaa
     8521 taaggggatt gtgacctttg atgccaaggt aaaaagcgct gatgtggata tccactcgag
     8581 ttatggtggt gttgtggaat cagctccttg gtatctcctc caagccttac agtctcttcg
     8641 tgctgcggat ggccgtatct tggttgaagg cttgtacgaa gaagtacaag agcccaatga
     8701 acgagaaatg gccttgctag aaacttatgg tcaacgaaac ccagaggaag ttagtcggat
     8761 ttatggattg gagttgcctc tcttacagga ggagcggatg gcctttctaa aacgtttctt
     8821 tttcgatcca gcgcttaata tcgaaggaat ccagtctggt tatcaaggtc agggtgttaa
     8881 gactatttta cctgcagaag ccagtgccaa gctagaggtt cgtctggttc cgggcctaga
     8941 accgcatgat gttctggaaa aaattcggaa acagctagac aaaaatggct ttgataaggt
     9001 agaattatac tataccttgg gagagatgag ctatcgaagc gatatgagcg caccagccat
     9061 tctcaatgtg atcgagttgg ccaagaaatt ctatccacag ggcgtttcag tcttgccgac
     9121 gacagcgggg acaggaccta tgcatacggt ctttgatgcc ctagaggtac caatggttgc
     9181 attcggtcta ggaaatgcca atagccgaga ccacggtgga gatgaaaatg tgcgaatcgc
     9241 tgattattac acccatatcg aattagtaga ggagctgatt agaagctatg agtagagata
     9301 ttatcaagtt agatcagatc gatgtgactt ttcaccaaaa gaagagaacc atcacagcgg
     9361 ttaaggatgt gaccattcac atccaagaag gggatatcta cggaatcgtt ggatattctg
     9421 gagcaggaaa atcaaccctt gtacgggtga ttaatctctt gcaaaaacca tctgcaggga
     9481 aaattaccat tgacgacgat gtgatttttg acggcaaggt gaccttgacg gcagagcagt
     9541 tgcgtcgtaa acgtcaagat atcggaatga ttttccagca ttttaacctg atgagccaaa
     9601 agacagcaga ggagaatgta gcctttgccc ttaaacactc tgaactcagc aaggaagaaa
     9661 agaaggctaa agtagctaag ttgttggact tggttggttt ggcagatcgt gctgaaaact
     9721 acccttcaca actatctgga gggcaaaaac agcgtgtggc aattgcgcgt gccttggcca
     9781 atgatccaaa aatcttgatt tcagacgagt caacttctgc ccttgatccg aagacaacca
     9841 agcagatttt ggccttgttg caagatttga accaaaaatt aggcttgact gttgtcttga
     9901 ttacgcatga aatgcagatt gtcaaagaca ttgccaaccg tgttgcagtt atgcaggatg
     9961 ggcatttgat tgaagagggt agtgtgcttg aaatcttctc aaaccctaaa caacctttga
    10021 ctcaagactt tatctcaaca gctacaggta ttgacgaagc catggtcaaa atcgagaagc
    10081 aagaaatcgt ggaacacttg tctgaaaaca gtctcttggt gcaactcaag tacgctggag
    10141 cttcaacaga cgagccactt ttgaatgaat tgtacaagca ttaccaagta atggctaata
    10201 ttctctatgg gaatatcgaa attctcgatg gtactcctgt tggagaattg gtggtggttt
    10261 tgtcaggtga aaaagcagcg ttggcaggtg cccaagaagc cattcgtcaa gcaggtgtac
    10321 aactaaaagt attgaaggga gtacagtaag atggaatcat tgattcaaac ctatttacca
    10381 aatgtctata agatgggttg ggctggtcag gcaggctggg gaacggctat ctacttaact
    10441 ctttatatga cagttctttc cttcattatc ggaggcttct tggggctagt ggcaggtctc
    10501 tttctcgtct tgacagcgcc aggtggtgtc ttggagaata aagtcgtatt ctggatttta
    10561 gacaaaatta cctcaatttt tcgtgcggtt ccctttatca tcctcttggc aatcttgtca
    10621 ccactttctc acttgattgt taaaacaagt atcgggccaa atgcagccct tgtcccactt
    10681 tcttttgcag tctttgcctt ctttgcccgt caggtgcagg ttgtcttggc tgaattggat
    10741 ggcggtgtca ttgaggcggc tcaagcgagc ggagcgactt tctgggacat cgtgggtgtt
    10801 tacctatcag aaggtcttcc agatttgatc cgtgtgacga ctgtgacctt gatttccctt
    10861 gttggggaaa cagctatggc cggtgcggtt ggagctggtg gtatcggtaa cgtagccatc
    10921 gcttatggat ttaaccgcta caatcacgat gtgaccatct tggcaaccat tattatcatt
    10981 ttgattatct ttgcaatcca attcttggga gatttcttga ctaagaaatt gagccataaa
    11041 taaaaaagag ccgcgtggct ctttttaact gatcagattt tctgggcaaa ttttttactc
    11101 aaggcttgtc caatcaaggc acccactagg gctccgatga caatacttgc gataaataga
    11161 aggacagttc cagggtttgg agcgaccatg atacggtcga tatattcttg ggattttcct
    11221 cttgccagaa gagtagccat ataggctttg ggcgcaatcc acataagcaa gattggtcct
    11281 gttgtactaa aggcgaaaat aatgaaagaa aggaagttct ttgttttgtc cttgtatttt
    11341 cctaaatgag ctactccatc tgctaggagg ccacagataa ttccaggaag gaaggaacca
    11401 gcaccgtgtt tagttcccaa gaaaaagagg gcaatgacaa ggccgatagt ggtaatggct
    11461 ccaaagcgcg gaacttttgc gactaggatc atatagacgc taccgccgac aagggcagta
    11521 aaggcaggcg cataaaacat gtttccagtt tggtcaaaga gattgcccaa aaggacacca
    11581 atccccatgc agaggaagta aagaactgca aaaagcagtg tagttaagat agatttcttc
    11641 atgaattgtc tcctgataat tttttcacaa ttctcatttt atcatggttt ggtgggattg
    11701 taaatggata ctagaagttt ttgaaaacgc ttgaaatatg atataatggt ttcatagtta
    11761 tttttagggg aatatcatgg gtagattttt agactttgtc tttaatcgtt tctttttagg
    11821 gatgattgcg acagccttct tttggctatt aactttagca ggagggatta tccttggtct
    11881 agcgccggct agtgccacct tgatgagctt atatgcagaa catggttata gctttcggga
    11941 atacagtttg aaggaggctt ggtctcttta caagcaaaat tttgtctcaa gcaacctgat
    12001 tttctatagc tttttaggtg tgggtctagt tttgacctat ggtttgtatc tcttggtgca
    12061 attgcctcat cagaccattg ttcatttgat tgcgaccctt ttgaatgtcc tagtagttgc
    12121 cctgatcttt ttggcttata cagtatcttt aaaattacaa gtttattttg ccttgtccta
    12181 tcgaaatagt ctcaaattat ccttgattgg catctttatg agtctagcag ctgtggctaa
    12241 ggttctcctt gggactgtgc tacttgtagc aattggttat tatatgcctg ccctgctatt
    12301 ttttgtagga attgggatgt ggcatttctt tatcagtgat atgttggaac ctgtctatga
    12361 aatcatccat gaaaaattgg cgacaaaata gaatgaagca gttttggcta catacgcttc
    12421 taagaaccta tagttcagtg atgatcatta tcattgcgag ttttgcaatc ttactctctt
    12481 acgctgactg ggattcacgt gaaaaggaag cccagagagt agcccagcgt gtaactgcta
    12541 gaacagtgag tgaaattgaa tattaccata gagagtcaac ccagatagct caggctttag
    12601 ttgaaaatca agctcgtatc gagggaatct ataaatactt tagccttagc atgccagact
    12661 atttttactg gcaattagag cggaaagctt cgccttatat atcagtctct ctgtatgaaa
    12721 atgttgatga cctctatgtt cgaaatgatt ttgtaactgg ggtggccatt gcttttcaag
    12781 attacaagga agtctatgtt tctactaaag acaaacgtag tggagaaaaa atcagggctg
    12841 aggatttcaa accagcagga aatagttttg ccattccagt gtcagatcca gtgtcagatc
    12901 aagacttagg agtgatttac atctccttgg atcctgctgt tttataccat gccattgata
    12961 atactagagg tcatactccg atggcagtaa cagtgacctc accttttgat acggagattt
    13021 ttcatatggg tgagacagtt gataaggaga gtgaaaattg gctagttggc ttaacttctc
    13081 atggttatca ggttcaggtg gcagttccca aaaactttgt tttacaagga acggtgacca
    13141 gctctgcttt gattgtgggc ttgagccttc tctttattgt cattctttat ctgactttga
    13201 ggcagacctt tgctaattat caaaagcagg tagtggattt ggtggattcc atccaagcta
    13261 ttgcccaagg acaagaaggt cttcgcattg atacgcttga aaaggatcag gaattgctcc
    13321 taatcgcgga gacgaccaat gatatgttgg atcgattgga aaagaatatc catgatattt
    13381 accagttaga actcagtcaa aaagatgcca atatgcgggc cttgcaggcg caaatcaacc
    13441 ctcacttcat gtataatacg ctggagttct tgcgtatgta cgcagtcatg cagagtcaag
    13501 atgagttggc agatatcatt tatgaattca gtagtctctt gcgtaacaat atttccgacg
    13561 aaagagagac cctcctcaaa caggaattag aattttgccg taaatacagc tatctctgca
    13621 tggttcgcta tcccaagtcc attgcctatg gtttcaagat agatccagag ttagagaata
    13681 tgaagattcc caagtttacc ttgcaaccgc tggtagaaaa ctatttcgcg catggtgttg
    13741 accacaggcg gacagataat gtgattagca tcaaggctct taaacaggat gggtttgtgg
    13801 aaattttggt ggtcgataat ggtagaggaa tgtcggctga aaagttggca aatatccgag
    13861 aaaaattaag tcagaaatat tttgaacacc aagccagcta cagtgatcaa aggcagtcta
    13921 tcgggattgt caatgtacac gagcgttttg tgctctattt tggagaccgc tatgccatta
    13981 ctatagagtc tgcagagcaa gccggtgttc agtatcgtat tacaattcaa gatgagtaga
    14041 aagggagaaa atgtataaag tattattagt agatgatgag tacatggtga cagaaggtct
    14101 gaagcgtttg attccctttg ataagtggga tatggaggtc gtcgcaacag ccagtcatgc
    14161 cgatgaagct ctagaatatg ttcaggaaaa tcctgtcgat gtcatcattt ccgatgtcaa
    14221 tatgccagac aaaacagggc ttgatatgat tcgggagatg aaagagatct taccagatgc
    14281 tgcctatatc ctgctctcag gttatcagga gtttgattat gtaaaaagag caatgaacct
    14341 tagtgtggtg gactatttgg tcaagcctgt tgataaggta gagctgggaa atctgctgga
    14401 gaagattgca ggtcagctcg gcgagagagg gaagaaaagt cagactctta gtcaagaatt
    14461 agacgaggct ggatttgtta gttatttagg ggataaggag aattggtgga taggtctatc
    14521 caaggaaaaa caaggttcct tcaccattcc ctactatgtc ttgggtcaag actggcagat
    14581 tttcatttct ggccaccccc tagatggttt agtcgttaca ccttttgaag ctccttatca
    14641 agaacatttt gaacgctgga agctgaatgc tgagaaaacc ctcttttacg gttctgtaaa
    14701 tctgcagcag tctgagagtc tctttgccta ttacgaaccg atttataggg ttatcattca
    14761 gggaaatctc aatcaaatcg tagaagagtt aaatctcttg gagaaggtag ttcttgaaaa
    14821 tacacctcgt gtttcgatta ctaaacagct ttttatccag tttgtcatgg atgttttcca
    14881 tttatttgaa catctcaaag ctgatgatat gacggacatt gtcaaaacca ttcatgctat
    14941 tcaatccttc gatgaattgg tttcttatat caaggaaact ctgatcagct ttttcgatca
    15001 ataccgtatg aatgaaaatg tggtcagtgt gctggaagtc attggtcgtg attaccaaaa
    15061 agagctttcc ctcaaggata tcagtaaggc cttctttatc aatcctgtct atctagggca
    15121 gttgattaag cgtgaaaccg attcgacctt tgcagagtta ctaaataaac aacgtattaa
    15181 ggctgcccag cagctcttgc tttcaactag tgacagcatc gaagatattt gttatgctgt
    15241 tggttacagt aaccttggat atttctataa agttttccga aaattgtgcg gaaaatcgcc
    15301 aaaagcctac cgaaaacagg tagaaactat actataagat ttgtattcct ttacaaaagg
    15361 tgctataata ttatcaggag gtcctatcat gaaaaagaaa ccaatttatc tatgggtttt
    15421 gctaatcttg tctgccctta tttcagttcc gtctctgttt ggaattgtga gtcccttgcc
    15481 tagtaaagaa gcccttcgtg ctgctcagaa acaagttgca ggggtcaatg ctcagcaatt
    15541 agaagaccag cttaattata cctatagagt agcagaagca tctcattcta tttttaatgt
    15601 tgctttgatt gtgctatcta ctattttagt ggtcgtagcg attgtcttcc ttgttcgtaa
    15661 aaatttgcaa tatgcaaact atacttacgt tggctatgtt ttgttagcta ttattggttc
    15721 gatttatggc tatgttggtt tacaagacgc tgtgcagttg gttcaagatg agagcatgcg
    15781 tttgacagtg agtattggtt caaaagctgt tagcattttc tatatcgtca tcaatgttct
    15841 cttcctagcc cttgtcttct ataagatgtg gcgacaacag aaagccttgg cagaagaaga
    15901 ggaaacagaa gagcttacct aagtattgac aaaaaagaac tatcaagata caatcttggt
    15961 agttgttttt tgattataga taaaatcatg gaggtacata tgaagacaat ttctttagtt
    16021 tatattagtt tgagtggcaa cactgaaagt tttgtgacgc gcttgaaaga ctatctcttg
    16081 tcccagtaca aagggattga ggttcaaaag attcatatca aggatttggt caaggaaggc
    16141 aaaaatttct atgaaatgga ccatccctat gtcgcttttt tgccgaccta tctcgaaggt
    16201 gggaatggcg tggataacgg agatgttgag attttgacga caccggtggg agattttatt
    16261 gcctatggta acaatgctag taagtgtttt ggtgtggttg ggtcaggaaa tcgtaacttt
    16321 aataaccaat actgcctaac agccaagcaa tacagtcaac gctttggctt ccctgtattg
    16381 gctgactttg aaatgcgagg catgctggaa gatatcaaac atgtcgcagc tattatcgca
    16441 gatttgtatg agttggaaaa ggagaattaa tactcaatga aaatcaaaga gcaaactagg
    16501 aagctagtcg caggttgctc aaagcacagc tttgaggttg cagatagagc tgacgtggtt
    16561 tgaagagatt ctcgaatagt ataaagttct tataagaggt ggttgaagtt gttcaactgt
    16621 tttttgagta taaacagtct ttaagaacgt aaaaaagcat aagttcaggt cttcaaaacc
    16681 ttgttcttat gctttttatc atagaaatat ttgctgaatt ttctcttgaa atgaaacctt
    16741 atcttcatct cgcgcttgtc ccttaccctg cttttccgat tgcgagtgcg tagataaaga
    16801 agatggcgaa gccaaagagg aaaatcaatc ccgcaatggc aaggtgtttg agccaagaag
    16861 gaagattttt gttttcacgc gttaccaagg cgtaattcaa aagagcaaag aaaggtgttg
    16921 tcaggaaaga gccaatcatg gcaaagcgga gcatggttga aacctgacca gcgaagaact
    16981 tgataatgac gataccgatg atagcagtga tggtcatcca gatgttcaaa gatttacgat
    17041 tgtcctcttt ttgactgatt agcagtcgga gagattcctg attaacgcga gaatagccat
    17101 cgataacagt tataactgtt ccaaagatac agaggaaggc aataaaggta atcaagtaac
    17161 gggaccattc gccaagaaca gaggcataca tgcccacgaa ttgagagatg tatttggctg
    17221 aagcagcttc aaccgcctgc cctgtaggat actgaatcag tgctcccagt gccacaaaga
    17281 agacggctag gatagctgtt ccaatataac cagtgttaaa gtcaaacaga gcgtcttctg
    17341 tgttaaagtt gacggtcttt ctcttttcag ctgaccaaag tgaattgatg gctgaaattt
    17401 caataggagc cggcatccat cctaggaggg agacgatgaa gggcagagct gccatttgcc
    17461 aaggtgtctt ctcgacaaaa tcagaactgt attctggatg cttgaccgcc gcaatgataa
    17521 ctgcaagaac agtcgcaatg gttaaggcag acataatcca tttgaccatg ccgtctaaaa
    17581 gtttgtagcc tccaaagagt agcatagccc aaatgattgc aacgagaatg agggaccact
    17641 gagtaatgct aagtccaatc attgggaaag cactggcgat gatagctgag cacagaatgg
    17701 caacaccagc cgtgttgacc atagccgaaa agacattgag gataaagaaa atccagagat
    17761 agagttttcc tttttcggca taaccttcaa ccaaagtctt tccagtatca gctgtgtatt
    17821 cagcaccaaa acggaaaaat ggatatttaa agacattggc taagatgacc aagagaagta
    17881 gagaccaacc gtaagaaccg ccagcttgag ttgaggatac aatgtgggaa cctccaacag
    17941 cggcagttgc cattaggatt ccaggtccca ttgcttttag cttgcttgcc caggttgatt
    18001 gatttaaggt aatagcttgc gacatgataa atactccttt ttgaattttc agaataatct
    18061 gtatatgtaa tctatattct acaaaaaatt ttggaaaaat gcaagaatat tttgaaaaaa
    18121 gatagaaaat ttacaaagaa aatctgaaat aattgttatg atatagcaga ttgaaactag
    18181 aatagtacac ctttgcttct aaaacattgt tagaaatcta tttgactgtc ctgatcgatt
    18241 tatcctgttc ttatttcatt tcactatagt aaggtgcagc aggataaaga aaaaccgaga
    18301 agtttctttc tcggatttgc ttattatgag gttcctattt tctatgctcc aagagttgat
    18361 tgatgtgatc tactttttta gcctctcttt tatagagaat agcccagatg atgagataga
    18421 caatcgcaaa ctcgataatg agctggaggt agaaaatcca gtggaaaggg aaccaaccag
    18481 caagagttgc tagtgggaca aagccagcca gcataaggaa gaaatgagtc agagtggcac
    18541 gaagcatgct ccagtcacgg ctaaataagc ggttgccaaa gttgaagagc ataccgatgg
    18601 ttgcccagat aagtgtgcag tagagcaaga ccagggcacc gtgaacctga tgctgatcca
    18661 tcacttggcc gatgagagag tagggattta gtggtgcgta ggtatttggt gcataaatga
    18721 gtgaaaagag gatagagagg atgaggccga taagaacacc ggtagctgca tcgtgaaata
    18781 cttgtttttt catagttcta atttctcctt gatggttttt agataacggc gtgaagagta
    18841 ggtgaagctt tcgtttttca agaaaatttc taccagaccg tttggcgtga gcttgaggtg
    18901 agagatggaa tcgatattga tgatttctga ttgggaaatt tggataaaat tggttggcaa
    18961 gagtttaaga acctgataga gtcgcaaatc aatgctgtag gtctgactcg cggtttctgc
    19021 tagaaccttc cgattctcga tatagaagcg ctgaatcttg ccaatctcaa ctagatagac
    19081 ctgatcatcg atttttcctt tgattttttc tctttggtcc agattttctg cgaactcgat
    19141 gactttctgg actttttcgg tttcttgagg tgcttggaca atcagctttt cctcctcgta
    19201 agtctcacta atctgtagtt ctactttcat agttttctct ccttttcagt tatacaaggt
    19261 tgtgatcact tcctgtatat ccttattaaa cactatttta tagcccatgg caaggaacct
    19321 tgtctatgtg gagggattgg gctcctatgt ggtggagctt ttctgttctt tctgaaatat
    19381 ggtataatag cactaatcaa tttctaggaa aatagatacg gaaaggggct gaaagaacta
    19441 ggggaacagt tggaattaga aagtgatagg tatgaaaagt ggcgttgaag ctgaataagt
    19501 ttataaagcg tttgaatgaa gaaatggaaa gtaaataagt tgacgatgaa ttacaaaata
    19561 gattttatct acaatttgta tctttcaaat cattgatttg aattttagat ttaatcatgt
    19621 tacaattaaa actgaaatca aatagaagag gaaaatgcaa acgtgggcac actattagca
    19681 acaagattaa aaaatagacg gaaagaatta aaaatgtctc agcgagaact agctgaaggt
    19741 atctgtaaac aggggcaaat cagtcgatta gagagcggag agtttacccc tggagcggat
    19801 ttcctgaatg cccttgctaa gaagttaaaa gtcagtatgg attatttttt tgatgagcag
    19861 gttgtagaga aagttgagga gttatcagaa tttaagaaat tagcacagac atttattaca
    19921 aatcgcaatt atgagtcttt gaaatatata tatgaattgg agaatgtaaa agctcaccgc
    19981 ctatctttag cagataaaat ctacatggaa tggataaaat ctctgataga tttttatttt
    20041 tacggacaga aagaagatgc gattgtacga ttagaggggg tattatcaca gttaagtgta
    20101 tctgatttga actatctcca agtttcaaac accctattca atttttatta tgatattgga
    20161 gatttaggtc gtttcaataa aattcgtgaa aaactagagt atcaagtaaa tcacctcaat
    20221 ttgagtacaa tcgaagagtt agaactatct attaagttta actacaacgt ttgtcgttat
    20281 ctatggttac agaaaaatat tgaagaagct attactaaaa ttacagctac tataaaacaa
    20341 tgtaaggctt acagaacgaa ttatcttttg gctgatttgt acttattgat gggaaatgta
    20401 agtgaaaatt tttcttctaa aagttcggtg aaagaatatt ttgagacggc atattttcta
    20461 tacaaacttg aggagaacat gtcaatgact ctgaaag
//
LOCUS       DAVHEW010000028        20480 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVHEW010000028 DAVHEW010000000
VERSION     DAVHEW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.028"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            28..1350
                     /locus_tag="VVL53_000939"
     CDS             28..1350
                     /locus_tag="VVL53_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4183132.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLSGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            1352..3238
                     /locus_tag="VVL53_000940"
     CDS             1352..3238
                     /locus_tag="VVL53_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU4183133.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            3406..3750
                     /locus_tag="VVL53_000941"
     CDS             3406..3750
                     /locus_tag="VVL53_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4183134.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            3760..5172
                     /gene="lacG"
                     /locus_tag="VVL53_000942"
     CDS             3760..5172
                     /gene="lacG"
                     /locus_tag="VVL53_000942"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU4183135.1"
                     /translation="MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            5241..6920
                     /locus_tag="VVL53_000943"
     CDS             5241..6920
                     /locus_tag="VVL53_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU4183136.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKV
                     INDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWTFPGPLGLLIGTNF
                     QLISFVFLSLILVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIIPGEIDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(7046..8485)
                     /locus_tag="VVL53_000944"
     CDS             complement(7046..8485)
                     /locus_tag="VVL53_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU4183137.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(8489..9838)
                     /gene="trkA"
                     /locus_tag="VVL53_000945"
     CDS             complement(8489..9838)
                     /gene="trkA"
                     /locus_tag="VVL53_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU4183138.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            10026..10874
                     /locus_tag="VVL53_000946"
     CDS             10026..10874
                     /locus_tag="VVL53_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU4183139.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10889..11437
                     /locus_tag="VVL53_000947"
     CDS             10889..11437
                     /locus_tag="VVL53_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU4183140.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            11444..11701
                     /locus_tag="VVL53_000948"
     CDS             11444..11701
                     /locus_tag="VVL53_000948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183141.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKQCFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            11803..13485
                     /locus_tag="VVL53_000949"
     CDS             11803..13485
                     /locus_tag="VVL53_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183142.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            13470..14300
                     /locus_tag="VVL53_000950"
     CDS             13470..14300
                     /locus_tag="VVL53_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU4183143.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            14493..14996
                     /locus_tag="VVL53_000951"
     CDS             14493..14996
                     /locus_tag="VVL53_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU4183144.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      15050..15284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15449..16012
                     /locus_tag="VVL53_000952"
     CDS             15449..16012
                     /locus_tag="VVL53_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4183145.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            16026..16652
                     /locus_tag="VVL53_000953"
     CDS             16026..16652
                     /locus_tag="VVL53_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4183146.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            16680..17069
                     /locus_tag="VVL53_000954"
     CDS             16680..17069
                     /locus_tag="VVL53_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU4183147.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            17074..17523
                     /locus_tag="VVL53_000955"
     CDS             17074..17523
                     /locus_tag="VVL53_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183148.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            17651..18238
                     /locus_tag="VVL53_000956"
     CDS             17651..18238
                     /locus_tag="VVL53_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU4183149.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            18628..20235
                     /locus_tag="VVL53_000957"
     CDS             18628..20235
                     /locus_tag="VVL53_000957"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU4183150.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6054 a         3522 c         4578 g         6326 t
ORIGIN      
        1 taaaaaataa aaaaaggagt tccggtgatg aacaatattt tagcgttttt agaaacaaaa
       61 gtcgctccgt ttggtgaaaa agttggcaac caacggcatt tgaaagctat tcgtgaagga
      121 tttatgatgg caatgccttt gattttagtc ggctctttat ttcttattct aatcagttgg
      181 cctcaagagg cttttacaaa ttggctgaat agtgttggat tgctaagtat cttgacaact
      241 atgaatcagt caacagtagc gattatctcc ttggtcgctt gtttcggtat tgcctacagg
      301 ttgtcggaag gatatggtac agatggtccg tcggcaggga tcatagcctt atccagtttt
      361 gtattgatgg cacctcgttt ttcgagtatg gtttatgata aaaatgggga gcaggtcaag
      421 cagttatttg gcggcgcaat accattttct agcctgaatg catcttcttt gtttatggcg
      481 attactattg gattggttac agcagagatt tatcgtatgt ttatccagcg cggaattacg
      541 ataaaaatgc caagtggtgt cccagatgta gtaagtaaat cattttcagc tcttttatct
      601 ggttttacta cttttgtttt gtgggctttg gtcttaaaag gtcttgaagc ggcaggagtt
      661 gcaggaggtc tcaacggact cctaggtgca attgttggaa caccgcttaa gttaattgca
      721 ggaacgcttc caggtatgat tctatgtgtt attgtaaact cattcttttg gttctgtgga
      781 gttaatgggg gacaagtttt aaatgctttt gtagacccag tttggttaca atttactaca
      841 gaaaaccaag aagctgtggc tgcaggacaa acactccaac acattattac attaccgttt
      901 aaagatttat ttgtatttat tggtggcggt ggagcgacta ttggtcttgc gatttgtctc
      961 ttcctattta gtaagagtcg tgcgaataaa acattaggta agctagctat tataccgtct
     1021 atttttaata tcaatacagc tattctattt acgtttccaa cagttttaaa tccgattatg
     1081 ctgattccgt ttattgctac tcctacaatc aatgccttga ttacctatgt atcaatggct
     1141 gtaggattag taccctatac aacaggtgta atccttccgt ggacaatgcc accgattata
     1201 ggaggcttcc ttgcaacagg ggctagttgg cgaggagctc tattacaagt tgttttgatt
     1261 ttggtttctg tagcaattta ttatccattc ttcaaaattg cagataaacg caatcttgaa
     1321 aaagaaaaag ctactgttgg agggaaataa gatggttatc agagtatttg atcaacagaa
     1381 aaatacttat tctagctttg ccttagagga attaagttac tatatgaatc gggtctttaa
     1441 gactaacata gagcttgtcg aggagaagga agcggatatt tttgtaggat tagtcaataa
     1501 agaggacaga aaagaccatg ttcttatctc attagacaag ggtaagggga gaattgagtc
     1561 taatacaatt gtaggtttac ttattggaat ttaccgaatg tttcatgaat ttggggttgt
     1621 gtatactaga ccagggcgca gacatgactt tgttccagag ttacgatttg aagatttttt
     1681 agataaacag ctatctatag atgaaacagc cagttactat cataggggag tatgtataga
     1741 gggagcggat tcatttgaaa atatactaga tttcattgat tggctaccta agattgggat
     1801 gaacagtttt ttcatccagt ttgaaaatcc ttactctttt ttgaaacgtt ggtatgaaca
     1861 tgaatttaat ccatatctaa ataaagaaca attttcaaat gaattagtac aagaattgag
     1921 tgataggttg gataaagaat tgcaaaaaag aggtcttatt catcatcgtg ttggtcatgg
     1981 atggacaggt gaagttttag gttactcttc aaaatttggc tgggaatcag gtcttagtat
     2041 ttcagaggag aagaaaccct atgtcgctga aataaacggg aaacgagaat tgtttaatac
     2101 ggctccgatt ttaaccagcc tggatttttc aaatccagat gtagctgata agatggtaga
     2161 aattatcaag gattatgcca agaaaagacc tgatgttaac tacttacatg tatggttgtc
     2221 ggatgctcgt aataatattt gtgaatgcga aaactgtaga caagaattgg tttcggatca
     2281 gtatattcgt attctcaatc aattggatag ggctttaacg agtgagggat tagatacaaa
     2341 gatttgtttt ctgctttatc atgagttgtt atgggcacct cagaaagaaa aattagataa
     2401 tcctgaacgc tttaccatga tgtttgcacc gattacaaga acatttgaaa tgagttatgc
     2461 agatgtagat tttgacaatt ccatacctac gcctaaacct tatatgcgta ataaaattat
     2521 acttccgaat tctcttgagg aaaatttatc ttatcttttt gagtggcaaa aagcatttaa
     2581 aggagatagt ttcgtatatg actatccttt agggcgtgct cattatggcg atttaggcta
     2641 tatgaaaatt agtcaaacta tttacagaga tgtatcttat ctttccaacc tacatttgaa
     2701 cgggtacatt tcgtgtcaag aattacgtgc cggattccct cataattttc ctaattatgt
     2761 catgggggaa atgctctgga agaagacaag aagttatgaa gaattgattg aagaatactt
     2821 ttctgctttg tatggggaaa attggcagtc tgttgttgaa tatttagaaa aattatccat
     2881 ttattcctct tgtgattatt ttaatgcaat tggcagccgt caaagtgatg ttttagcgaa
     2941 tcattattat atagcttaca atctagctga taatttttta ccaattattg aggaaaatat
     3001 ttctaagtta ttaaatagtc aaaaggatga atggaaacag ctcagttatc atcgtgaata
     3061 tgttgttaag atggcgaagg ctttatatct tcaagcaact ggaaaaacaa ggcaagctca
     3121 agatgaatgg agaaatgtgt tgaattatat ccgtgggcac gaattgctat ttcaatctaa
     3181 tttggatgtt tatcgtgtaa ttgaagtagc aaaaaattac gctggtttcc acttataaat
     3241 cataagtata gaaaatgaac taaggtattc agagaagatt gatcctaaat attatgaaat
     3301 ttaaggattt ttaagatatt tagggtcaac tttctattta tatcgtagcg aagtcatttt
     3361 aataatgatg tgtaaaagat ggatcaagat tgaggaggaa gaaagatgaa atcaaaagaa
     3421 gaaataaata tgcttggttt tacaattgtc gcttacgcag gagatgcaag gtcagatttg
     3481 atggatgctt tggcgtttgc gagagatgga tattttgaac aggcaagaga attggttgag
     3541 tctgcaaacg actcaatagt gtctgcccat cgagaacaga ctaatttatt agcggaggag
     3601 gcatatggag ataattttga agtgagcttt attatgattc atggtcaaga tactttgatg
     3661 acaacgatgc tattgtatga tcaggtaaag ttttttattg atgaatatga acgaattcga
     3721 aagattgaag aacatattgg tttgcaatga ggattagtca tggaaaattt acaggttaaa
     3781 gccttaccga aggagttttt attaggaact gctaccgctg cttatcaagt agagggtgca
     3841 actagggtag atggcaaagg aataaatatg tgggatgttt atttgcaaga aaatagtccg
     3901 ttcttaccag atccagctag tgatttttat tatcgttacg aagaggatat agctttggcg
     3961 gcagaacatg gtttgcaggc tttgcgttta tctatttctt gggttcgtat atttcctgat
     4021 atagatgggg atgctaatgt attagctgtt cattattacc atagagtttt tcagtcttgc
     4081 ttaaaacata atgtgattcc gtttgtttct ttacatcatt ttgattcgcc tcagaaaatg
     4141 ttagaaacag gggattggtt gaacagagag aatattgatc gtttcatacg atatgctcgc
     4201 ttttgtttcc aagaatttac agaagtcaag cattggttta caatcaatga actgatgtct
     4261 cttgctgcag gtcaatatat aggaggtcag tttcctccaa atcatcattt tcaattatct
     4321 gaagcaattc aagcgaatca taatatgttg ttggcgcatg ctcttgcagt cctcgaattt
     4381 catcaattag ggattgaggg aaaggtaggt tgtattcatg ctttaaagcc aggctatcct
     4441 attgatgggc aaaaagaaaa tattttggca gctaaacggt atgatgttta taataataaa
     4501 tttctattag atggaacttt tttgggctac tacagtgagg acacgctttt tcacttgaat
     4561 caaatattgg aagctaataa ttctagcttt attattgaag atggtgattt agaaattatg
     4621 aagagagctg cacctcttaa tacgatgttt gggatgaatt attatcgttc agaatttatt
     4681 cgtgaataca aaggtgaaaa tagacaagaa tttaattcaa caggaataaa aggacagtct
     4741 tcttttaaat taaatgctct aggtgaattt gtaaaaaaac ctggtattcc gacaacagat
     4801 tgggattgga atatttatcc tcaagggtta tttgatatgt tgcttcgtat caaagaagaa
     4861 tatcctcaac atccggtcat ttatttaact gaaaatggta cagcccttaa agaagttaag
     4921 ccagagggcg agaatgatat tattgatgac agtaagagaa tccgttatat tgagcaacat
     4981 ttacacaaag ttttagaggc tcgagataga ggagtcaata ttcaaggcta ttttatatgg
     5041 tctttgcaag atcaattttc ttgggcgaat ggctacaata agcgatatgg tcttttcttt
     5101 gttgattatg aaacacagaa gagatatatt aagaaaagtg ctctttgggt aaaagggcta
     5161 aaacggaatt aaggttagcg atttgactga tgtttaatat gttttaaata tgaggttgaa
     5221 ttttttatag gaggagtttt atggataagc tagtcgctgc cattgaaaag caacaaggga
     5281 aatttgaaaa aatttctact aataactata tgatggctat taaagatgga ttcattgcta
     5341 ctatgccttt aattatgttt tcaagctttt tgatgattat tattatgatt cctaaaaatt
     5401 tcggagtaga gttaccgagt ccagctattg tctggatgag aaaagtgtat atgttaacca
     5461 tgggagtttt gggtattatt gtttcaggga ctgttggaaa gtcattagtt ggaaatgtta
     5521 acagaaaaat gcctcacgga aaggtaataa atgatatttc tgcaatgttg gcagccatat
     5581 gtagttatct ggtattaact gtaacgcttg tagttgatga gaagacggga tctacaagtt
     5641 tgtcgacaaa ctatttagga tctcaaggat tgataacttc gtttgtcagt gcctttatta
     5701 ctgtaaatgt ttaccgattc tgtattaagc gagacattac tattcattta cctaaggaag
     5761 ttcctggggc tatatcacaa gcttttagag atattttccc tttttctttt gttttactta
     5821 ttagtggttt gttagatatt gtatctcggt ttagtttaga tgttcctttt gcccaagtat
     5881 ttcaacaact attgactcct atttttaagg gggcagaatc atatcctgct atgatgttga
     5941 tttggtttat gtgtgctttg ctttggtttg ttggaattca tggaccatct attgtcttac
     6001 ctgctgttac agctttgcaa ctgagcaata tggaagagaa tgctcaactt cttgcaaatg
     6061 ggcagttccc ttatcattct ttaacaccta atttcgggaa ttatatcgct gctattggag
     6121 gaacgggggc tacctttgtt gtaccattta ttttgatttt ctttatgcgg tctaaacaat
     6181 taaaatcggt aggtaaagct acaattactc ctgttttatt tgcggtaaat gaacctcttc
     6241 tatttggtat gcctgttatt ttgaatccct atctttttgt cccttttttg atgactccac
     6301 cagtgaatgt atttctagga aaggtcttta ttgatttctt tggaatgaat ggattttata
     6361 tccagttacc ttggaccttt cctggtccct tgggattgtt aattggaacg aattttcaac
     6421 ttatctcctt tgtattttta tctttgattt tagttgtcga catattgatt tatttgccat
     6481 tctgtagagc gtatgataga cagttactgg tgaaagaaga tattgcaagc tcaaatgata
     6541 ttattttaga ggaggataca agtgaaataa ttcctggtga gatagatgaa ataaaaagta
     6601 aggagttgaa agtactggtt ctttgtgcag ggtctggaac aagtgcgcaa ttagccaatg
     6661 caattaacga gggcgctcaa ttagcagaag ttagagtgat tgcgaattca ggagcgtacg
     6721 gagctcatta tgatattatg ggtgtttatg atttaattat tctggcccca caagttcgga
     6781 gttattatag agagatgaag gtggatgcag aaagattagg tattcagata gttgctacca
     6841 gaggaatgga atatattcat ttaacaaaga gtccaagtaa agccttacaa tttgtattgg
     6901 agcattacca agctgtgtag taagtttttc catcttttat ttgagtaaag attttgctta
     6961 cagataggct tggatttaaa aacgttcccc cttttttaat ataagaatcc ctctttcaca
     7021 attgtaaaaa gagggatttt gtattttatc tcttagacca agttctcttc ataaagagaa
     7081 ggaggattgg gtaaatctcc aagcgccctg caatcattgc aaaggatagg agaatttttg
     7141 agatgggact aaagattgag aaactagaag tggttcctag aataggcccg atattattga
     7201 aacagctaaa gacagcgctg gtcacgacca gaaaatcatt gctatctagg ctgacaataa
     7261 agataagcgc tagcaaaatc atagcataga tgacaaagta cttgagaatc ttatgctggg
     7321 tatctttgtc aatcaccgtt tgattaacat ggagggtcaa aacacggtgg ggcgatagga
     7381 ttgacaaaat ttggtttttg gcaatttttg aaaggatgag gcctcgaata atcttgagtc
     7441 cacctgcagt tgatccagca gagccaccga ttgccatgag gaaaaggagg ataaactggg
     7501 agaagagggg ccagttggta atatctccat atccaaaacc agttgttgta atgatgttgg
     7561 aaacctggaa gaaggtcatt tcaaagctct ttgaaaaccc tgggtagagg tagagggtgt
     7621 tgaggctaat caagcctgta gaaaccagta caatgaccaa gtaagcccga agttcttcgt
     7681 caccaaagaa ggccttgatg cgacggagca tgaggtagta gtagaggttg aaatttaccc
     7741 caaaaaccag aactccgata ctgaccagat aggtaatcag tgagctgcca tagtgggcaa
     7801 ttccgtcgtt atagacggta aagcctccag ttcccgctgt ccccatagca atgacaaaac
     7861 tatcgtagag aggcatacca gctagataat agatgatgac aaagagggag aagagagcta
     7921 gataaaggag atagagaatc tgggcagtgt tttttagttt ggatacaacc ttgccaaaaa
     7981 caggacctgg aacctcagcc ttcatcacct ctaggtggct atttttggca ttgtccataa
     8041 tagcaagtgc aaaaacaagc actcccatcc ctccaatcaa gtgggtaaaa cttcgccaga
     8101 agaggaggga acggctgaga accgaaatgt cgttcaaaat acttgctcca gtagttgtaa
     8161 acccagaact aatttcaaaa aaggcatcaa taacgctagg gatttgccca gaaaagacaa
     8221 aggggagacc accaaagaaa gaccaaagga tccaacagag ggcaacgatc aagactccct
     8281 ccttggcata aatccgttga ttttttggct tctgtaaact ccctgaaccg cctaacaata
     8341 cgagaatccc tatggtcgaa aagagggctg taaagacttg gctcgattca cggtaataga
     8401 cagcgatagc cacaggaacc aagaggagaa cagcttcaat caaaagtaac tttgaaagga
     8461 ggtaacgaat catactttta ttcatttctt acctcgcaat caagtcataa atcttggtga
     8521 tgtttggcaa caaggttgtt actaggagct tgtctccaac ttccaacata tcctccccag
     8581 ttgggaaaat agtcttgccc tttcgaataa tggctgcaat aagaacccct tttttcaatt
     8641 tcagttgaga aagaggtttg gcagtcattt tattggcttc cttgatatgg aattgcaggg
     8701 tttcaatttg gccattggct agatggtgca tggcttgaag gtctgaatac tgggcattaa
     8761 ctcgaccacg aataaagtgc ataatcgtat ctacagcgat gcttttaggt gtgatgatac
     8821 ttgaaaaatc aggcgcattg ataatctcga ggagactggt acgattgacc ttagtaatat
     8881 ttttctgtac acctaccctg tcaaggaaca tagatgtaat cagattttcc tcatcgacac
     8941 ctgttagagt cgcaacggca tcatagtgtt gagcactttc ttccagcagg atatcttttg
     9001 cggttccatc tccttgaacg atgtagagat ttgggaattt ctcgctaaag aagctggcga
     9061 tttcaggatt gatttcaatg acttttgtat cgatacgact atctttgaga ataccaagta
     9121 gataataggc aattctacct gccccaacga tgagaaggct cttcacggcg cgtgatttaa
     9181 aataattatg gaagagtatc atatcgacac ggttaccagt gacaaagatt ctatctttat
     9241 cctgtacagt catgtcaccg cttggaatga taatttgatg atccctctct atcgcacaga
     9301 caatgacatt accaaatttt ttacgaaaat cagaaatggg catttggcaa agaccgctgg
     9361 tggacttgac gacaaattcc atgaggctaa cgcgtccacc agcaaagcgt tcgacagaca
     9421 gggcgttggg gaagtcaatg atattcgcga tagcgcgggc agccaagagc tcaggattaa
     9481 cgataagaga aaaaccgaga atattctttt ccttgaaata agagttagaa tattcagggt
     9541 tccgcacccg aacgatagtt tctttagctc ccattttctt ggctagaact gctgcaatca
     9601 tgttgacttc atcgtgctca gtcagggcga taaagatatc acaatcttgg acgctggctt
     9661 gctcaagaat ggcaaaatcg gccccgttac caaggatacc aatgatatca aagcgactga
     9721 caatatgatt gagaacagct tcgtcttgct caatcagcaa aacatcatgc ttttctgcaa
     9781 ccaaggagcg acagagggca aaaccaactt ttccccctcc gacaaggata attttcataa
     9841 taaaacctac tttttcatga tgtaactatc ataccctttt tcaagaaaaa atgcacctac
     9901 tagctaataa caagagcttt tagtgaaaat tcgctataag gtaaaactat accctaacca
     9961 attgaaatag ctattagcga ctttctctga aatatggtat gataaaggat atacaaggag
    10021 ataaaatgaa taataattta ctggtattac aatcagactt tggtctggtt gatggtgcgg
    10081 tatcggctat gattggagtg gctttagaag agtctccaac cttaaaaatc catcacttga
    10141 cgcacgatat cacgccttat aatatttttg aggggagcta tcgtctcttt cagacggtgg
    10201 attactggcc tgagggaacg acgtttgtat cggttgtcga tccaggtgtc ggttcgaaac
    10261 gtaagagtgt agttgccaag actgcaaaaa atcaatacat tgtcacgcca gataatggga
    10321 cgctttcctt tatcaagaaa cacgttggca ttgtagccat tcgtgagatt tctgaggtgg
    10381 ccaataggcg tcaaaacaca gagcattctt ataccttcca cggtcgtgat gtctatgcct
    10441 atactggtgc taaactggcc agtggtcaca ttacttttga ggaagtaggg ccagagctca
    10501 gtgtggaaca gattgtagag cttccagtcg tagcgaccat catagaagat catctggtga
    10561 agggagccat tgatattctg gatgtgcgtt tcggttcgct ttggacctct atcacacggg
    10621 aagaatttta caagctggaa ccagaatttg gtgatcgttt tgaagtgacc atctatcatg
    10681 ctgatatgct ggtctatcaa aatcaggttg tctatggcaa atcatttgca gatgtgagaa
    10741 ttgggcaacc catcctttac atcaactctc tctatcgttt aggtttggct atcaaccaag
    10801 gttcctttgc caaggcctat aatgtaggtg tcggttcatc ttggaccatt gaaataaaga
    10861 aaatagaagg ataaaatagg agaatggtat ggaaatcaaa tttacaatta aacaagttgt
    10921 tgctgtcgga attggcgctg ccctctttgt cgtcatcggg atgatcaaca ttccaacccc
    10981 tgttccaaat acaagcatcc agcttcagta tgcggtacaa gcgctacttt ctattatttt
    11041 tggaccgatt atcggtttgc ttgtcgggtt gattggtcat gcaatcaagg actctcttgc
    11101 tggttatggt ctatggtgga cttggattat cgctagcgga ctctttggtc tagttgtggg
    11161 actttttaga aagtacgttc gagtgatcaa tggtgttttt gactggaaag atattcttat
    11221 ttttaacctc attcaactac ttgcaaatgc ccttgtttgg ggtgtcttgg caccacttgg
    11281 agatgttgtg atttatcaag aagcggcaga aaaagtattt gcacaaggga ttgttgcggg
    11341 aattgccaat ggtgtatctg tagctattgc aggaactctt ctcttacttg cctatgcagg
    11401 aacccaaact cgtgcaggaa gtttgaaaaa ggactaatac tcaatgaaaa tcaaagagca
    11461 aactaggaag ctagccgcag gttgctcaaa acagtgtttt gagattgtgg atagaactga
    11521 cgaagtcagc tcaaaacagt gttttgaggt tgtagatgaa actgacgaag tcagtaacca
    11581 tacctacggc aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaatgat
    11641 ggggaaggct gggcaactag tctttttcga tgtttataga ttagttaggc aagtaagcta
    11701 ggataggaat ctttttggcg ctaagatttt aagagaagag actgcaagaa gggcttgatt
    11761 tatgataaaa tagaagtcta agaagcgaat gaagagagta agatgaaaga agctataatt
    11821 gagtggaagg atttctcttt ccggtatgaa acacaacaag aaccgacctt gcaagggata
    11881 gacttgacca tttacaaggg agaaaaagtc ttaattgttg gaccatctgg gtcaggtaaa
    11941 tctaccttgg gtcagtgttt gaatgggatt attcccaata tttacaaggg tcagacatat
    12001 ggagaatttt tgataaaggg tcaagtagcc tttgatatga gcatctatga taagtctcat
    12061 ctggttagca cagttttgca ggatacagat gggcagttta ttggcttgtc tgtggcagaa
    12121 gatttggcgt ttgctctgga aaatgatgtg acagccctag atgagatgaa aggtcgtgtt
    12181 tataaatggg ctgaaaagct ggaccttctt cctttactgg atcagcgtcc tcaggatttg
    12241 tcaggtggac aaaagcagcg agtcagtctg gctggtgtct tgattgatga aagtccgatt
    12301 ctcttgtttg atgagccact cgccaatcta gatcccaagt caggtcagga tattatcgaa
    12361 ttgattgacc agattcataa ggaagagggg acgacgactc tgattatcga gcaccgtttg
    12421 gaggatgttc tgcatcgccc tgtggatcgg attgtcttga taaacgatgg tcgtatcctt
    12481 tttaatggga gccctgacca gttgcttgcg actgatttat tgactcaaaa tggaattcga
    12541 gaaccccttt atctaacgac tctccgtcaa ttaggtgtgg acttagtcaa ggaagaacaa
    12601 ttagcgaatc tggataactt gtctatctca aaaggtcagg ttcagttgca gaatgaactg
    12661 gcaaaagaaa ccccagcatt gcagtcactc tttagactag aggaagtatc tttttcttat
    12721 gatgatagac cgattttaaa atccctacat ttagatatta aaaagggtga aaagattgct
    12781 attgtcggaa aaaatggagc agggaaatca actctagcca aggctataag tagctttatt
    12841 cagacggaag gacgctatct ttgggaaaaa caggatataa aaggcgattc tgttgcagag
    12901 cgggcggaac gagtaggcta tgtgctacaa aatcctaatc aaatgatttc aaccaatatg
    12961 atttttgatg aggtggctct agggctccgt ttgcgaggtg tggatgagaa ggaaattgaa
    13021 acgagagtct atgaaacctt gaaaatctgt ggactttatg aattccgtaa ttggcctatt
    13081 tctgccctgt catttggtca gaaaaaacgt gtcaccattg cttcaatttt ggtcttagga
    13141 gctgaaatta ttctcctaga tgaaccgact gcaggtcaag atcagaagaa ctatactgag
    13201 attatggaat ttctcgaaga gttacatcaa aaagggcata ccattgtcat gattacccat
    13261 gatatgcaat tgatgctgga ttattcagac cgggtccttg tcatggtgga tggagaattg
    13321 attgccgata ctgttccagc cagtctgttg agcgatcctg agctgttagt aaaagccaat
    13381 ctaaaagaaa cctccatctt taacttggct aagaaactag atgtggatcc actggattta
    13441 acggcatttt acaaagaaag gagagaggga tgcaagctaa attaatcggt taccagcata
    13501 gagatactgt gattcatcgc ttgtcaggag ctgggaaact tctctttttc attctggtat
    13561 cattggcggc catgattagc tatgatacca gaatgctctt gctgattgcc atcttttcag
    13621 tctttctcct ctatttatca gaaattcgct ttaaagatgt ttcctttgta gccgtttttg
    13681 cgacggtatt tgccgtttta aacgttttga tggtctatct cttttctccc gagtatgggg
    13741 ttggacttta tggagagaga agtgtgattt ggcagggaat cggtgtctac actctaacca
    13801 gccaggagct cttttatctg ctaaatttgg ccattaagta tctttgcacc attcctctgg
    13861 ctattatctt tttgatgaca acccatccta gtcagtttgc ttccagttta aatcaaattg
    13921 gtgtgcccta taagattgct tattctgtca gcctgacctt gcgctatatt ccagatttgc
    13981 aggaagaatt ctttactatc aagatgtctc aggaggcgcg tgggatggaa ttatccaaga
    14041 aagcttctct tatgcaacga atcaaaggca atctgctcat tattacgccc ttgattttta
    14101 gctcgctaga acgcattgat accattgcga ctgccatgga gcttcgccgc tttgggaaag
    14161 agaaaaaacg cacatggtat agttatcagg ccttgaaaaa aggagactat attaccttgc
    14221 tcttggcaac cttgttttta gtagttagtt tactacttat cttgcagaat cagggacgat
    14281 tttacaaccc ttggaaatag cttgaaaaaa ttgaaaaaat caagtcattt ctattgacaa
    14341 tgattctgaa agtgttatac taagaaagta gtttcgctga tttacttcaa acctgttgtg
    14401 aggtaagtta acgatgcctt aaccacgctg tttgctgagc ttgactccgg gcagtgtggc
    14461 tatttttttg caatggtgaa aggaagcaag tcatgacaaa tcacattgta ttatttgaac
    14521 ctcaaattcc acaaaataca ggcaatatcg cgcgtacttg cgctgcgacc aattctcccc
    14581 tccacatcat caagcctatg ggctttccta ttgatgaccg caagatgaag cgggctggat
    14641 tggattactg ggataagcta gagatttatt tttacgagag cttagaagat ttcatgtctc
    14701 agatgaaggg aaaactctat ctgatttcga aattcgcgga aaaaatctat tctgaggtgg
    14761 atttatcgac tgacgaggac cattattttc tctttggacg tgaagacaag ggcttacctg
    14821 aagattttat gcgagaacat cctgagaagg ctcttcgtat tcccatgaat gatgaacatg
    14881 tccgcagtct caatgtgtct aataccgtct gcatgattgt ctatgaggct cttcgccagc
    14941 agaactttgc aggtcttgaa ctcgtccata cctatgaagt ggataaattg aaataaaaaa
    15001 tgaaaatgaa caaaatgctt gcgcttgcaa gcgttttttg ttatgataaa agagtcttca
    15061 gggcagggtg tgattcccga ccggcggtga ctttaactag gaaatgatct tttcctttta
    15121 tactttgttg acaagctttg cctaaccaga agttatgcct acagcttgtc gcctagtcta
    15181 aaagaaaaat ctctatttcc ttctctttaa agaagtccgc gagcgcaagc tgatgtggtg
    15241 agattccaca accgacagta tagtctggat gggagaagac gaaagaatag ctttgtctgt
    15301 tttgatagat ttatagccgg attgtaacca cttgctttgt ttttctttaa tagagtaaga
    15361 gggaactttt gggatataaa aagtgagaat agatagagga atcctttcca acttcttctg
    15421 attttataga aaattggagg aacctgttat gacaaacaca cgtcgacttt cgaccattgc
    15481 aattctatca gccatctcat ttgtgctgat gtactttgac tttccgcttt taccagcggc
    15541 atctttcctc aagatcgaat ttagtatctt gccagtcctt gtgggtctgg tggtcatgga
    15601 tttgcctgct gctctaggag tgctcttgct tcgctcacta ttgaaattgc ttcttaacag
    15661 ccaaggagtg aatacttaca ttggtttgcc gatgaatatc gtagctttgg gagtttttgt
    15721 catcgtattt gctttgattt ggaaaaagga acggacaacc cttcgtttcc tactaggctc
    15781 tctagctgga actgttggtt taaccttggc catgttggtt ctcaactatg tttatgctgt
    15841 tcctttgtac gctaagtttg ctaactttga tattggaaaa attttgggac tttccaacta
    15901 cctaatgacc atggtattac cttttaactt gattgaaggt gttatctttt ccgtttcatt
    15961 ctggttgttg tatgttctct tgaaaccaac cttaaaacat tatgagagat aaacaaacat
    16021 ttttaatgaa gggcagtttt gcccttttac ttttcgttat tcttggctac atggtcaaat
    16081 tttaccctga aacgctggtc aattttgacc aatcgattca gactgccatt cgaggagact
    16141 taccagatta tttgactatt ctttttcgag ccctcacacg tctgattgat atcccagtga
    16201 ttatcacttg ggttgtcatt acagcttttg tcttttatcg taagcgatgg aagatagaaa
    16261 gtttcttcat gctgggaaat ctggctttgg caggtctttt aatcgtgacc tttaaaaata
    16321 tctaccagcg cccacgacca gctatcttac atctggtgga agagaaggga ttttccttcc
    16381 caagtgggca ttctctggct gtaactttga tggtagggac tttgattgtt attcttagtc
    16441 agcggattaa agatccagtc tggagaaaaa tcgtacaaat cgtccttggc ctctacctag
    16501 tcagtgtgct ggtatcaaga gtctatctgg gagttcatta cccatcagac gtccttgcca
    16561 gtctctgtgt gggcttggga gtcctgttta tcgagtttcc cttctatgac aagctccgct
    16621 tccaatggcg atttaaaggc aaacagaagt gagtatcgaa ttcccttgag gagaaagaaa
    16681 tgaaagtcaa tatagcagat cttcatccga ctcaactata tttatcagaa aagaagttgc
    16741 aagatattca gatgctttac cagtcggcag aaacaatcca agtcgatcca atcagtattc
    16801 ttgcctttgg agattgtttg ctgattacag atgggcatca cagggcttat caggctttat
    16861 tggcaggtcg ggatacgatt tctgctgagt gggatagaga tggtggtgat gaactatatc
    16921 atctctatgc gcaagcttgc gaggaaagaa agatttactc tgtttttgat ttagaagatc
    16981 gtatcctagc tcaagatggg tatgaagcaa aatggtataa ctggtgtgat ggttttaatc
    17041 aagcagcaac tcttttgttg aaaaggtagt gcattgtgtt ttgttcctta ttataaggtc
    17101 aattattgtg aggaagcttt tgcttggtat caggatgtga acttggttta cctcgtagat
    17161 ggggtgaagc ttccttacag tcaagcgacc ttggaagcca tgtattccta tttggatcgg
    17221 catggtgagc ttttttggat tgaagtcaag gagaagggag aatggtttcc aattggggat
    17281 attacactat ctcaggataa tctccccatt gtgattggga attccgctta ccaacatcga
    17341 ggacttggaa aaaagattct aagtgctttg attgaattgg ctcgagtaaa aggatggaaa
    17401 gaattgagag tcaaggaaat ctacacctac aatcatgctt ctaggaggtg tttcaagtcg
    17461 cttggatttg tggaaaatgg agcaacagaa aaaggaagga gttttatatt ggaattagtc
    17521 taatcctgcc actttctccc ccttaacctt tgactattcc gcaaaattat cgtaaaataa
    17581 agagtaaatg ataaaatgag gtcagagtct gttcgctctg gcgatagtag tataaatgag
    17641 gagaaacgct ttggaattag aagtatttgc tgggcaagaa aaaagtgaac tatctatgat
    17701 tgaggtagcg cgtgctatct tggaacttcg tggtcgcgat catgagatgc attttagcga
    17761 tcttgtaaac gaaattcaaa actaccttgg aacatcaaac agcgatatcc gcgaagcttt
    17821 gcctttgttc tacacagagt tgaactttga cggtagcttc atctcacttg gggacaacaa
    17881 atggggtctt cgttcatggt atggtgtgga cgaaatcgac gaagaaatca tcgctcttga
    17941 agaaaatgac gacgatgaag tagcaccaaa agctaagaaa aaacgtgtca atgcctttat
    18001 ggatggtgat tcagatgcca ttgactacaa tgcagatgat ccagaagacg aagatgcata
    18061 cgaagcagat ccagctcttt catacgatga tgaaaatcca gatgatgaaa aaaatgaagt
    18121 ggaagcttat gatgcagaaa tcaacgaaat cgctccagat gacttgggag aagatgtgga
    18181 tctcaacgaa gacgacgacg agttttcaga tgacgacgct gaaaccagcg aggaataaga
    18241 gttagctatt gacaattatc ctatttttag gtatcatatt gtttgggcac ctcttttaga
    18301 ggtcggggct ccctagttct tagggagcta tttttgtttt ttcaagaagt tatcttcttg
    18361 tattttatac tcaatgaaaa tcaaagtgca agctaggaaa ctagccgtag gctgctcaaa
    18421 acactgtttt gaggttgtag ataagactga caaagtcagg aacacatatc tacggcaagg
    18481 cgacgttgac gcggtttgaa gagattttcg aagagtatta gttgtgaatc tggtgcagtc
    18541 gtcccagatt attcttatta gtagggtctt gttttctata tcccctcgta gttaacaaga
    18601 ccttgagcat tttagaaaga ggaatctatg tctacgaaat atatttttgt aactggtggt
    18661 gtggtatcgt ctattgggaa agggattgtg gcagcgagtc taggccgtct cttgaaaaat
    18721 cgtggtctca aagtaaccat tcaaaagttt gacccttata tcaatattga tccgggaacc
    18781 atgagtcctt accagcacgg ggaagttttt gtgacagatg acggagctga gacagatttg
    18841 gacttgggtc actatgaacg tttcatcgat atcaatctca acaaatattc caacgtgaca
    18901 actgggaaaa tttacagtga agttcttcgt aaagaacgcc gtggagaata ccttggggca
    18961 actgttcaag tcattcctca tatcacagat gctttgaaag aaaaaatcaa gcgtgccgct
    19021 ctaacgaccg actctgatgt cattatcaca gaggttggtg gaacagtagg agatatcgag
    19081 tccttgccat tcctagaggc tcttcgtcag atgaaggcag atgtgggtgc ggataatgtc
    19141 atgtatatcc atacaacctt gcttccttac ctcaaggctg ctggtgaaat gaaaaccaaa
    19201 ccaacccaac actctgtcaa agaattgcgt ggcttgggaa tccaaccaaa tatgttggtt
    19261 attcgtacag aagagccagc tggtcaagga attaaaaata aactggccca gttctgtgat
    19321 gtggcaccag aagccgttat cgaatcgttg gatgttgaac acctttacca aattccactg
    19381 aacttgcagg cacaagggat ggaccaaatt gtttgtgatc atttgaaatt agacgcacca
    19441 gcagcggata tgacagaatg gtcagccatg gtggacaagg tcatgaacct caagaaacaa
    19501 gttaagattt cccttgttgg taagtatgtg gagttgcaag atgcctatat ctcagtggtc
    19561 gaagccttga aacactctgg ctatgtcaat gatgtagaag ttaaaatcaa ttgggtcaat
    19621 gccaatgatg tgacagcaga gaatgtagca gaactcttgt ctgatgcgga cgggatcatc
    19681 gtaccaggtg gttttggtca acgtggtaca gaagggaaaa tccaagccat ccgctatgcg
    19741 cgtgaaaatg atgttccaat gttgggagtc tgcttgggaa tgcagttgac atgtatcgag
    19801 tttgctcgtc acgttttagg tcttgaaggt gccaattctg cagagcttgc accagaaaca
    19861 aaatacccta tcattgatat catgcgtgat cagattgata ttgaggatat gggtggaacc
    19921 cttcgtttgg gactttatcc gtctaagttg aaacgtggct ctaaggctgc tgctgcttat
    19981 cacaatcaag aagtggtgca acgccgtcac cgtcaccgtt atgagtttaa taatgccttc
    20041 cgtgagcagt ttgaggcagc aggttttgtc ttttcaggag tttctccaga caatcgtttg
    20101 gtagaaatcg tggaaattcc tgaaaataaa ttctttgtag cttgtcagta tcaccctgaa
    20161 ctgtcaagcc gtccaaaccg accagaagaa ctctacactg cctttgttac tgcagcggtt
    20221 gagaacagca attagcaaaa tcagaacctt tgagaaaaat ctcagaggtt ttttgcatac
    20281 gatgatattg cagtatatct gaggtaggag tcctctgtat gtacctgcta ccgttgaaat
    20341 caatagcgac tccctcttgc cctgtgctag tgaatggatt tatcagtata ttgaaatgaa
    20401 ataaaatttg aacaaattaa ttcggaaagc caaatcaatt tctagcaaag ttttaggaac
    20461 tggattgtat agtgaattga
//
LOCUS       DAVHEW010000029        20372 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVHEW010000029 DAVHEW010000000
VERSION     DAVHEW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.029"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            63..605
                     /locus_tag="VVL53_000958"
     CDS             63..605
                     /locus_tag="VVL53_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU4183151.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(646..719)
                     /locus_tag="VVL53_000959"
     tRNA            complement(646..719)
                     /locus_tag="VVL53_000959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Asn,seq:gtt)
     gene            complement(725..796)
                     /locus_tag="VVL53_000960"
     tRNA            complement(725..796)
                     /locus_tag="VVL53_000960"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Glu,seq:ttc)
     gene            complement(848..1600)
                     /locus_tag="VVL53_000961"
     CDS             complement(848..1600)
                     /locus_tag="VVL53_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183152.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1597..2922)
                     /locus_tag="VVL53_000962"
     CDS             complement(1597..2922)
                     /locus_tag="VVL53_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4183153.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVIFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2943..3068)
                     /gene="comC"
                     /locus_tag="VVL53_000963"
     CDS             complement(2943..3068)
                     /gene="comC"
                     /locus_tag="VVL53_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU4183154.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            complement(3233..3306)
                     /locus_tag="VVL53_000964"
     tRNA            complement(3233..3306)
                     /locus_tag="VVL53_000964"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3270..3272),aa:Arg,seq:tct)
     gene            complement(3350..3829)
                     /gene="rlmH"
                     /locus_tag="VVL53_000965"
     CDS             complement(3350..3829)
                     /gene="rlmH"
                     /locus_tag="VVL53_000965"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU4183155.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4012..5193
                     /locus_tag="VVL53_000966"
     CDS             4012..5193
                     /locus_tag="VVL53_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4183156.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5251..6009
                     /locus_tag="VVL53_000967"
     CDS             5251..6009
                     /locus_tag="VVL53_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU4183157.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6222..7583
                     /gene="dnaA"
                     /locus_tag="VVL53_000968"
     CDS             6222..7583
                     /gene="dnaA"
                     /locus_tag="VVL53_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU4183158.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            7742..8878
                     /gene="dnaN"
                     /locus_tag="VVL53_000969"
     CDS             7742..8878
                     /gene="dnaN"
                     /locus_tag="VVL53_000969"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU4183159.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            8943..9137
                     /locus_tag="VVL53_000970"
     CDS             8943..9137
                     /locus_tag="VVL53_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU4183160.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            9221..10336
                     /gene="ychF"
                     /locus_tag="VVL53_000971"
     CDS             9221..10336
                     /gene="ychF"
                     /locus_tag="VVL53_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU4183161.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELNDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            10407..10976
                     /locus_tag="VVL53_000972"
     CDS             10407..10976
                     /locus_tag="VVL53_000972"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU4183162.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            10977..14486
                     /gene="mfd"
                     /locus_tag="VVL53_000973"
     CDS             10977..14486
                     /gene="mfd"
                     /locus_tag="VVL53_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU4183163.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            14544..14810
                     /locus_tag="VVL53_000974"
     CDS             14544..14810
                     /locus_tag="VVL53_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU4183164.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            14803..15171
                     /locus_tag="VVL53_000975"
     CDS             14803..15171
                     /locus_tag="VVL53_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU4183165.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            15291..16559
                     /locus_tag="VVL53_000976"
     CDS             15291..16559
                     /locus_tag="VVL53_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU4183166.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            16556..17833
                     /gene="tilS"
                     /locus_tag="VVL53_000977"
     CDS             16556..17833
                     /gene="tilS"
                     /locus_tag="VVL53_000977"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU4183167.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            17837..18379
                     /gene="hpt"
                     /locus_tag="VVL53_000978"
     CDS             17837..18379
                     /gene="hpt"
                     /locus_tag="VVL53_000978"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU4183168.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            18395..20353
                     /gene="ftsH"
                     /locus_tag="VVL53_000979"
     CDS             18395..20353
                     /gene="ftsH"
                     /locus_tag="VVL53_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU4183169.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         7046 a         3292 c         3787 g         6247 t
ORIGIN      
        1 aaattacaag ataaaaaatc tatttttaga catatagtct aatttgttta caaggaggaa
       61 atatgcaaga aagtaacaaa cgcttaaaaa caaagcgaac tattgaaaat gctatggtac
      121 aattactgat ggaacagcca tttgataaaa tttctactgt caaattagta gaaaaagccg
      181 gaattagtcg ttccagcttc tatactcact ataaggataa gtatgatatg attgagcact
      241 atcaaagcaa gctattccat acatttgaat atatttttca aaaacatgct catcacaaaa
      301 gagacgctat tttagaagtt tttgaatatc tagagtcaga accacttctg gctgcccttc
      361 tttctgaaaa tgggactaaa gaaatccaaa atttcttacg aaataaactt catatcatgc
      421 ttagtacaga tttacaaaag cgatttatgc aactgaatct aaataccact gaattagaat
      481 atagtagcat ctatctaact catgcacttt ttggtgtctg ccaaacttgg attgcacatg
      541 gaaaaaaaga aagttctcaa gaaataacag acttccttat gaaaatgctt ggtgatacaa
      601 attgatacaa aaagaggaac acagctgtgt tcccttttat ctatactccg ccagtaggac
      661 tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc tatggcggat
      721 aaaatagtcc gtacgggatt cgaacccgtg ttaccgccgt gaaaaggcgg tgtcttaacc
      781 ccttgaccaa cggaccttct atctgtagca gatataacca ttatatcaat ttcttgctaa
      841 ttgtcaatca cttttgagat tttttctcta aaatatcttt taattttcta atttttaatc
      901 ttgaaatagg acaacgatgg tcttcataga aaacaatttc taagtttttt cgatcaattt
      961 ctctgatatt acctatattt accaaaaatg acttgtgagg agaataaaat cgctgagtat
     1021 gtttgtcctt ttcctgaata tctgtcatgg taccataaaa ctcttttgca aaattcttac
     1081 caataatgcg caatttatga gatacccctg ttgtttcaat atacaaaata tcatggtaag
     1141 gaatttttaa atcatttccc ttgtaattgt agtcgaaata atctacaaca tcttcatttt
     1201 caagtaacat actcttcgtg tagaagatat tttgctcaat tctcttctta aacatctcat
     1261 cattgatatc cttatcaaca aaatctaggg ctgatacctg gtatttatag gttagagtcg
     1321 caaactctga tcgactagtg ataaagacga taatagcgta aggattgtaa tgacgaatga
     1381 gctgagccac ttcaaatccc tttttctcaa ttccatgaat atcgatatct aggaaataaa
     1441 gctgatttac ttcatcattt tcaatgtatt cttcaaattc acggactttt cccgttgtct
     1501 tgtatgatat tggaatattc gattctttcg aaatttcatc caatattctc tctagtctca
     1561 cttgatgttc aataacatct tctaaaatta aaactttcat tcaaattccc tcttaaatct
     1621 aatgatttgt ctaaatgtac tgccttccat ctctgtttct aaaataatat tgttgtactt
     1681 atctagtagt tctttcacat tatttaatcc gactccgcga tttcttccct tagtggagaa
     1741 tcctaaggca aatagatctc ctgaaggagt catcgtcatt ttacatgaat tctgaatcac
     1801 aataactgtt tcagtttcca tcttaataac tgctacttcc atctgctttt tatagctatc
     1861 agccgatcct tcgacagcat tattcaataa aacgctcatg atacgaacca aatccaatag
     1921 ttcaattgga agcttggtaa tcgtatcttt tacttccagt gtaaactcta caccattatt
     1981 tcgagcatag acaattgact gagcaaccaa acttcgtaaa gctgagtctt ctatgttgtt
     2041 caaatcaaag taagtgtact tatctgaacg caatttatga tttgctttga ctaaaacttc
     2101 attgtaaatt ctgtcaattt cctgtaaatt accactgtca attgccatct gcatgctgac
     2161 aagcattcca gcataatcat gtcgaaaacc acggatttca ttatacagac caacaatttc
     2221 atctgtgtaa ttctgtaaat gtttctgttc aaatttcttc tgcttcaaag caatctcttt
     2281 ctccatttga actttatgag aattcattgc aaagaaggtc aaaaggagag agataaagac
     2341 aatagatgac aaaatacttc caaaactatt caaatgttta atcgtactta ccatatctga
     2401 aacgaaagat acaatatgta gcaatagtaa agcaaaaaat acttttttca agaaaggata
     2461 aaggtagtcc ttgtcaaaat aggctagttc caaatggaaa tagtaaatga tttttaatgt
     2521 aacaaaatag gttaacaccg tcacaacgaa aaagaatggg aaatgatatt gtaaaacaaa
     2581 attatctcct gttatagagg agaaaattac ggacagaaag ttatgagtgc tctcatataa
     2641 aagagatagt agtaaactta ggaatagtcc tctatccctc tcatactgtt tcatccatcg
     2701 aaaataggaa tataagccca aaggaaataa aaatctttca atccctattt tatctaaata
     2761 tagaagataa aaggaaaatt caagtactat ttcagttagt aatgtataag caccaaaaac
     2821 gtataattct tttctattta ttcgaccttt acaaattaaa cggtaactgt gactaataat
     2881 taaaaaatga acaataactg tcccaaatcc aagtaaatcc attactcttt ctccttattt
     2941 cattactttt ttcgtaggaa aagaaaatca aggatgattc ttgaaatcct catctcccca
     3001 cctttaatct tttgtaagtc tttttccttc aaagctacaa actgttccaa tttaactgtg
     3061 tttttcataa taaaatctcc taaaatgttt tttcttgtaa gctaacttac aaaaaccatt
     3121 atacaaaatg gaatttcgtt ttagataaaa ttctctcaac tgtcattttt ttctcccaaa
     3181 gtgtactttt ttaagaaaaa agccgggaaa attcccagct ttgctattat attgatccca
     3241 gcaggattcg aacctgcgac cgttcgctta gaaggcgaat gctctatcca gctgagctat
     3301 gagacctaat acaattattc taccaaaaat tcaattaaaa gtcaattttc tatttatggt
     3361 aggggaatcc ctgctgaatc gtaaaagcgc gatagatttg ttcaacaaga actagtctca
     3421 ttaactgatg gggtaaggtt aggcgaccaa aactgacaga aagattggct ctatttttta
     3481 cagatgatga taatcctaaa cttcccccaa taataaaagt aagagtagaa aatcctttta
     3541 tagaagtttc ttctaactgc ttactaaatt cttctgagaa gaaagttttc ccttcaatgg
     3601 ctaacacaat aacgaaatca cggtcagcaa tttttgataa aattctctga ccttctattt
     3661 ctaaaatctt ttgattttct gattcactgg ccttatctgg tgttttttca tctgataact
     3721 caatcatttc aaacttagca aatctagaaa ttcgttttga atactctgcg ataccatctt
     3781 ttaaatactt ttctttcagt ttcccaactg ttacaacttt aattttcatg actctattct
     3841 aacatattct ctattttttc acatcttatt cacaaaataa aaaatagatt tcaattaaga
     3901 aaatcacaat ttcaaaagag ttatccacag tttgtgtaaa acttttgtgt ttaagttata
     3961 attaagctag tcagtttata ctttcagtaa ttcaaacata tggaggcaaa tatgaaacat
     4021 ctaaaaacat tttacaaaaa atggtttcaa ttattagtcg ttatcgtcat tagctttttt
     4081 agtggagcct tgggtagttt ttcaataact caactaactc aaaaaagtag tgtaaacaac
     4141 tctaacaaca atagtactat tacacaaact gcctataaga acgaaaattc aacaacacag
     4201 gctgttaaca aagtaaaaga tgctgttgtt tctgttatta cttattcggc aaacagacaa
     4261 aatagcgtat ttggcaatga tgatactgac acagattctc agcgaatctc tagtgaagga
     4321 tctggagtta tttataaaaa gaatgataaa gaagcttaca tcgtcaccaa caatcacgtt
     4381 attaatggcg ccagcaaagt agatattcga ttgtcagatg ggactaaagt acctggagaa
     4441 attgtcggag ctgacacttt ctctgatatt gctgtcgtca aaatctcttc agaaaaagtg
     4501 acaacagtag ctgagtttgg tgattctagt aagttaactg taggagaaac tgctattgcc
     4561 atcggtagcc cgttaggttc tgaatatgca aatactgtca ctcaaggtat cgtatccagt
     4621 ctcaatagaa atgtatcctt aaaatcggaa gatggacaag ctatttctac aaaagccatc
     4681 caaactgata ctgctattaa cccaggtaac tctggcggcc cactgatcaa tattcaaggg
     4741 caggttatcg gaattacctc aagtaaaatt gctacaaatg gaggaacatc tgtagaaggt
     4801 cttggtttcg caattcctgc aaatgatgct atcaatatta ttgaacagtt agaaaaaaac
     4861 ggaaaagtga cgcgtccagc tttgggaatc cagatggtta atttatctaa tgtgagtaca
     4921 agcgacatca gaagactcaa tattccaagt aatgttacat ctggtgtaat tgttcgttcg
     4981 gtacaaagta atatgcctgc caatggtcac cttgaaaaat acgatgtaat tacaaaagta
     5041 gatgacaaag agattgcttc atcaacagac ttacaaagtg ctctttacaa ccattctatc
     5101 ggagacacca ttaagataac ctactatcgt aacgggaaag aagaaactac ctctatcaaa
     5161 cttaacaaga gttcaggtga tttagaatct taattgacat ctatgtaaag aaagctttac
     5221 ataagagaaa agatgtgtta gtgtagaatc atggaaaaat ttgaaatgat ttctatcaca
     5281 gatatacaaa aaaatcccta tcaaccccga aaagaatttg atagagaaaa actagatgaa
     5341 ctagcacagt ctatcaaaga aaatggggtc attcaaccga ttattgttcg tcaatctcct
     5401 gttattggtt atgaaatcct tgcaggagag agacgctatc gggcttcact tttagctggt
     5461 ctacggtcta tcccagctgt tgttaaacag atttcagacc aagagatgat ggtccagtcc
     5521 attattgaaa atttacagag agaaaattta aacccaatag aagaagcacg cgcctatgaa
     5581 tctctcgtag agaaaggatt cacccatgct gaaattgcag ataagatggg caagtctcgt
     5641 ccatatatca gcaactccat tcgtttactt tccttgccag aacagattct ttcagaagta
     5701 gaaaatggca aactatcaca agcccatgcg cgttccctag ttgggttaaa taaggaacaa
     5761 caagactatt tctttcaacg gattatagaa gaagatattt ctgtaaggaa attagaagct
     5821 cttctgacag agaaaaaaca aaagaaacag caaaaaacta atcatttcat acaaaatgaa
     5881 gaaaaacagt taagaaaact actcggatta gatgtagaaa ttaaactatc taaaaaagac
     5941 agtggaaaaa tcattatttc tttttcaaat caagaagaat atagtagaat tatcaacagc
     6001 ctgaaataag gctgttcttt tattttttta tctcacaagg ttatccacta tgtttttcga
     6061 taaaaagctt aataaatcaa taatttcttc ttttatcccc aacctgtgga taaagtttgg
     6121 taacattgtg gattattttt cacagcttgt ggaaaattct tgctatctat ggtaaaatat
     6181 ctctagtatt aaacttttaa atagtaaagg aggagaaagg attgaaagaa aaacaatttt
     6241 ggaatcgtat attagaattt gcacaagaaa gactgactcg atccatgtat gatttctatg
     6301 ctattcaagc tgaactcatc aaggtagagg aaaatgttgc cactatattt ctacctcgct
     6361 ctgaaatgga aatggtctgg gaaaaacaac taaaagatat tattgtagta gctggttttg
     6421 aaatttatga cgctgaaata actccccact atattttcac caaacctcaa gatacgacta
     6481 gctcacaagt tgaagaagct acaaatttaa ctctttataa ctatagtcca aagttagtat
     6541 ctattcctta ttcagatacg ggattaaaag aaaagtatac ctttgataac tttattcaag
     6601 gggatggaaa tgtttgggct gtatcagccg ctttagctgt ctctgaagat ttggctctga
     6661 cctataaccc tctttttatc tatggaggac caggccttgg taagactcac ttattaaacg
     6721 ctattggaaa tgaaattcta aaaaatattc ctaatgcgcg tgttaaatat atccctgccg
     6781 aaagctttat taatgacttt cttgatcacc taagacttgg ggaaatggaa aagtttaaaa
     6841 agacctatcg tagtcttgat cttttgttaa tcgatgatat ccagtcactc agcggaaaaa
     6901 aagtcgcaac tcaggaagaa tttttcaata cctttaacgc ccttcatgac aagcaaaaac
     6961 agattgtcct aacgagtgat cgtagtccaa aacatctaga agggctcgag gagaggcttg
     7021 tcacgcgttt tagttgggga ttgacacaaa ctatcacccc ccctgacttt gaaacacgta
     7081 ttgccatttt acaaagtaag acggaacatt taggctacaa tttccaaagt gatactctag
     7141 aatacctagc tgggcaattt gattcaaatg ttcgagatct tgagggagcc atcaacgaca
     7201 tcactttaat tgccagagta aaaaaaatca aggatatcac tattgatatt gctgcagaag
     7261 ccattagagc ccgcaaacaa gatgttagcc aaatgctcgt catcccaatt gataaaatcc
     7321 aaactgaagt tggtaacttt tatggtgtta gtatcaaaga aatgaaggga agtagacgcc
     7381 ttcaaaatat tgttttggcc cgtcaagtag ccatgtattt atctagagaa ctaacagata
     7441 atagtcttcc aaaaattggg aaggaatttg ggggaaaaga tcataccaca gtcattcatg
     7501 cccatgccaa aataaaatct ttgattgatc aagacgataa tttacgttta gaaattgaat
     7561 caatcaaaaa gaaaatcaaa taatttgtgg ataactttta gttttttatc ttttttatcc
     7621 acatttttta aacaagctaa aaaacttgat atgacttgtt taaaggctgt tttccacaga
     7681 tttcacagac tctattatta ctattatctt tctaatacta aaaataaata aaggagaatc
     7741 catgattcat ttttcaatta ataaaaattt atttctacaa gcattaaata ctactaagag
     7801 agctattagt tctaaaaatg ccattcctat tttatcaaca gtaaaaattg acgtgaccaa
     7861 tgaaggtatt actttaattg gttcaaatgg tcaaatttca attgaaaatt ttatttctca
     7921 aaaaaatgaa gatgctggtt tgttaattac ttctttaggt tcgatccttc ttgaagcttc
     7981 tttctttatc aatgtagtat ctagtttacc tgatgtaact cttgatttta aagaaattga
     8041 acaaaatcaa attgttttaa ccagtggcaa atcagaaatt accctaaaag gaaaagatag
     8101 cgaacaatat ccacgaatcc aagaaatttc agcaagcact cctttaatac ttgaaacaaa
     8161 attactcaag aaaattatta atgaaacagc ctttgctgca agtacacaag agagtcgtcc
     8221 gattttaaca ggtgtccact tcgtattgag tcaacacaaa gagttaaaaa cagttgcaac
     8281 agactctcat cgcctaagcc agaaaaaatt gactcttgaa aaaaatagtg atgattttga
     8341 tgtcgtaatt cctagccgtt ctctacgcga attttcagcg gtatttacag atgatatcga
     8401 aactgtagag attttctttg ccaataacca aatcctcttt agaagcgaaa atattagctt
     8461 ctatactcgt ctcctagaag gaaactatcc tgatacagat cgcttgattc caacagactt
     8521 taacactact attactttta atgtggtaaa cttacgccag tcaatggagc gtgcccgtct
     8581 tttatcaagt gcgactcaaa atggtactgt gaaacttgaa attaaggatg gggttgttag
     8641 cgcccatgtt cactctccag aagttggtaa agtaaacgaa gaaatcgata ctgatcaggt
     8701 tactggtgaa gatttgacca ttagtttcaa cccaacttac ttgattgatt ctcttaaagc
     8761 tttaaatagc gaaaaggtga ctattagctt tatctcagct gttcgtccat ttactcttgt
     8821 gccagcagat actgacgaag acttcatgca gctcattaca ccagttcgta caaattaagt
     8881 gaaagaggtt gagcctggct cgcctctttt atgatataat cgaaaaagaa aaggagagta
     8941 gtatgtatca agttggaaat tttgttgaga tgaaaaaatc acacgcttgt acaatcaagt
     9001 cgactggtaa aaaggctaat cgttgggaaa ttacacgtgt aggagcagat atcaaaataa
     9061 aatgtagtaa ttgtgagcat gttgtcatga tggggcgata tgattttgag cgaaaaatga
     9121 ataaaattat tgactgagaa cccttagtta gagggttagc actttatccc tttttgtgtt
     9181 ataatattag ggattgaaat gaaaacggag aatgagaaat atggctttga cagcaggtat
     9241 cgttggtttg ccaaacgttg gtaaatcaac actatttaat gcaattacaa aagcaggagc
     9301 agaggcagca aactacccat ttgcgacgat tgatccaaat gttggaatgg tggaagttcc
     9361 agatgaacgc ctacaaaaac taactgaaat gataactcct aaaaagacag ttcccacaac
     9421 atttgaattt acagatattg cagggattgt aaaaggagct tcaaaaggag aggggctagg
     9481 gaataaattc ttggccaata ttcgtgaagt agatgcgatt gttcacgtag ttcgtgcttt
     9541 tgatgatgaa aatgtaatgc gcgagcaagg acgtgaagac gcctttgtag atccacttgc
     9601 agatattgat accattaatc tggaattgat tcttgctgac ttagaatcag tgaacaaacg
     9661 atatgcgcgt gtagaaaaga tggcacgtac gcaaaaagat aaagaatcag tagcagaatt
     9721 caatgttctt caaaagatta aaccagtcct agaagacggg aaatcagctc gtaccattga
     9781 atttacagat gaggaacaaa aggttgtcaa aggtcttttc cttttgacga ctaaaccagt
     9841 tctttatgta gctaatgtgg acgaggatgt ggtttcagaa cctgactcta tcgactatgt
     9901 caaacaaatt cgtgaatttg cagcgacaga aaatgctgaa gtagtcgtta tttctgcgcg
     9961 tgctgaggaa gaaatttctg aattgaatga tgaagataaa aaagagtttc ttgaagccat
    10021 tggtttgaca gaatcaggtg tagataagtt gacgcgtgca gcttaccact tgcttggatt
    10081 gggaacttac ttcacagctg gtgaaaaaga agttcgcgct tggactttca aacgtggtat
    10141 gaaggctcct caagcagctg gtattatcca ctcagacttt gaaaaaggct ttattcgtgc
    10201 agtaaccatg tcatatgaag atctagtgaa atacggatct gaaaaggccg taaaagaagc
    10261 tggacgcttg cgtgaagaag gaaaagaata tatcgttcaa gatggcgata tcatggaatt
    10321 ccgctttaat gtctaaaaat taataaatgg tgtcaattag gttggaaaaa aattccaacc
    10381 cttttggctt ttgaaaggaa aaataaatga ccaaattact tgtaggcttg ggaaatccag
    10441 gggataaata ttttgaaaca aaacacaatg ttggttttat gttgattgat caactagcga
    10501 agaaacagaa tgtcactttt acacacgata agatatttca agctgaccta gcatcctttt
    10561 tcctaaatgg agaaaaaatt tatctggtta aaccaacgac ctttatgaat gaaagtggaa
    10621 aagcagttca tgctttatta acttactatg gtttggatat tgacgattta cttatcattt
    10681 acgatgatct tgacatggaa gttgggaaaa ttcgtttaag agcaaaaggc tcagcaggtg
    10741 gtcataatgg tatcaagtct attattcaac atataggaac tcaggtcttt aaccgtgtta
    10801 agattggaat tggaagacct aaaaatggta tgtcagttgt tcatcatgtt ttgagtaagt
    10861 ttgacaggga tgattatatc ggtattttac agtctgttga caaagttgac gattctgtaa
    10921 actactattt acaagagaaa aattttgaga aaacaatgca gaggtataac ggataaatgg
    10981 tgaccttatt agatttattc tcagaaaatg atcagattaa aaaatggcat caaaatttaa
    11041 cagataagaa aagacaacta atacttggtt tatcaacatc tactaaggct cttgcaattg
    11101 caagcagttt agaaaaagaa gataggattg tgttattgac gtcaacttat ggagaagcag
    11161 aaggacttgt tagtgatctt atttctatct tgggtgagga actcgtctat ccatttttgg
    11221 tagatgatgc tcctatggtg gagtttttga tgtcttcaca ggaaaaaatt atttcacggg
    11281 ttgaagcctt gcgttttttg actgattcat ctaagaaagg gattttagtt tgtaatatcg
    11341 cagcaagtcg attgatttta ccgtctccca atgcattcaa agatagtatt gtaaaaatct
    11401 cagttggtga agaatatgat caacacgcgt ttatccatca gttaaaggaa aatggctatc
    11461 gaaaagttac tcaagtacaa actcagggcg aatttagtct tcgaggagat attttagata
    11521 tttttgaaat atcccagtta gaaccttgtc gaattgagtt ttttggtgat gaaattgatg
    11581 gtatcaggtc atttgaagta gaaacacaat tatcgaaaga aaataagaca gaactcacta
    11641 tctttccagc tagtgatatg cttttgagag aaaaggatta tcaacgagga cagtcagctt
    11701 tagaaaaaca aatttcaaaa actttatcac ctattttgaa atcataccta gaagaaattc
    11761 tttcaagttt tcaccaaaaa caaagtcatg cagactctcg gaagttttta tctttgtgct
    11821 atgataagac atggactgtc tttgattata ttgaaaaaga tactccaata ttctttgatg
    11881 attatcaaaa attgatgaat cagtatgaag tctttgaaag agacttagcg cagtacttta
    11941 cagaagaatt acagaatagt aaagcatttt ctgatatgca gtatttttct gatattgaac
    12001 aaatctataa aaaacaaagt ccagtgacct ttttctctaa tcttcaaaag ggtttaggaa
    12061 atctcaaatt tgacaaaatt tatcaattca atcaatatcc tatgcaggaa tttttcaatc
    12121 agttttcttt tctaaaagaa gaaattgaac gatataaaaa aatggattac accattattc
    12181 tgcagtctag caattcaatg ggaagtaaaa cattggagga tatgttagag gaatatcaga
    12241 ttaaattgga ttctagagat aagacaaata tctgtaaaga atctgtaaac ttaatagagg
    12301 gtaatctcag acatggtttt cattttgtag atgaaaagat tttattgata actgaacatg
    12361 agatttttca aaagaaatta aagcgtcgtt ttcgaagaca acatgtttca aatgcagaga
    12421 gattaaaaga ttacaatgaa cttgaaaaag gggactatgt tgtccatcat atccatggga
    12481 ttggtcaata tctaggaatt gaaaccattg aaatcaaggg aattcatcgc gattatgtca
    12541 gtgtccaata ccaaaatggt gatcaaattt ctatccccgt ggaacagatt catctactgt
    12601 ccaaatatat ttcaagtgat ggtaaagctc caaaactcaa taaattaaat gacggtcatt
    12661 ttaaaaaggc caagcaaaag gttaagaacc aggtagagga tatagctgat gatttaatca
    12721 aactctactc tgaacgtagt cagttgaagg gttttgcttt ctcagctgat gatgatgatc
    12781 aagatgcctt tgatgatgct ttcccttatg ttgaaacgga tgatcaactt cgtagtattg
    12841 aggaaatcaa gagggatatg caggcttctc agccaatgga tcgactttta gttggggatg
    12901 ttggttttgg aaagactgaa gttgctatgc gtgcagcctt taaagcagtc aatgatcaca
    12961 aacaggttgt cattctagtt ccgacgacgg ttttagcgca acagcactat acgaatttta
    13021 aggaacgatt ccaaaatttt gcagttaata ttgatgtgtt gagtcgcttt agaagtaaaa
    13081 aagagcagac tgcaacactt gaaaaattga aaaacggtca agtcgatatt ttgattggaa
    13141 cacatcgtgt tttgtcaaaa gatgttgtgt ttgctgattt gggcttgatg attattgatg
    13201 aggaacagcg atttggtgtc aagcataagg aaactttgaa agaactgaag aaacaagtgg
    13261 atgtcctaac cttgaccgct acgccaatcc ctcgtaccct ccatatgtct atgctgggaa
    13321 tcagagattt atctgttatt gaaactccgc cgactaatcg ctatcctgtt cagacctatg
    13381 ttttggaaaa gaatgatagt gtcattcgtg atgctgtctt gcgtgaaatg gagcgtggag
    13441 gtcaagttta ttatctttac aacaaagttg acacaattgt tcagaaggtt tcagaattac
    13501 aggagttgat tccggaggct tcgattggat atgttcatgg tcgaatgagt gaagtccagt
    13561 tggaaaatac tctattagac tttattgagg gacaatacga tatcttggtg acgactacta
    13621 ttattgagac aggggtggac attccaaatg ctaatacttt atttattgaa aatgcggacc
    13681 atatgggctt gtcaacctta tatcagttaa gaggaagagt cggtcgtagt aatcgtattg
    13741 cttatgctta tctcatgtat cgtccagaaa aatcaatcag tgaagtctct gaaaagagat
    13801 tagaagcgat taaaggattt acagaattgg gctctggctt taagattgca atgcgagatc
    13861 tttcgattcg tggagcagga aatcttttag gaaaatccca gtctggtttc attgattctg
    13921 ttggttttga attgtattcg cagttattag aggaagctat tgctaaacga aacggtaatg
    13981 ctaacgctaa cacaagaacc aaagggaatg ctgagttgat tttgcaaatt gatgcctatc
    14041 ttcctgatac ttatatttct gatcaacgac ataagattga aatttacaag aaaattcgtc
    14101 aaattgacaa ccgtgtcaat tatgaagagt tacaagagga gttgatagac cgttttggag
    14161 aatacccaga tgtagtagcc tatctgttag agattggttt ggtcaaatca tacttggaca
    14221 aggtctttgt tcaacgtgtg gaaagaaaag ataataaaat tacaattcaa tttgaaaaag
    14281 tcactcaacg actgttttta gctcaagatt attttaaagc tttatccgta acgaacttaa
    14341 aagcaggcat cgctgagaat aagggattaa tggagcttgt atttgatgtc caaaataaga
    14401 aagattatga aattttagaa ggtttgctga tttttggaga aagtttatta gagataaaag
    14461 agtctaagga agaaaattcc atttgatatt tttcttctat aaaatagata aaaatggtac
    14521 aataataaat tgaggtaata aggatgagat tagataaata tttaaaagta tcgcgaatta
    14581 tcaagcgtcg tacagtcgca aaggaagtag cagataaagg tagaatcaag gttaatggaa
    14641 tcttggccaa aagttcaacg gacttgaaag ttaatgacca agttgaaatt cgctttggca
    14701 ataagttgct gcttgtaaaa gtactagaga tgaaagatag tacaaaaaaa gaagatgcag
    14761 caggaatgta tgaaattatc agtgaaacac gggtagaaga aaatgtctaa aaatattgta
    14821 caattgaata attcttttat tcaaaatgaa taccaacgtc gtcgctacct gatgaaagaa
    14881 cgacaaaaac ggaatcgttt tatgggaggg gtattgattt tgattatgct attatttatc
    14941 ttgccaactt ttaatttagc gcagagttat cagcaattac tccaaagacg tcagcaatta
    15001 gcagacttgc aaactcagta tcaaactttg agtgatgaaa aggataagga gacagcattt
    15061 gctaccaagt tgaaagatga agattatgct gctaaatata cacgagcgaa gtactattat
    15121 tctaagtcga gggaaaaagt ttatacgatt cctgacttgc ttcaaaggtg ataaaatgga
    15181 aaatttatta gacgtaatag agcaattttt gagtttgtca gatgaaaagc tggaagaatt
    15241 ggctgataaa aatcaattat tgcgtttaca agaagaaaag gaaaggaaga atgcgtaaat
    15301 tcttaattat tttgttgcta ccaagttttt tgaccatttc aaaagtcgtt agcacagaaa
    15361 aagaagtcgt ctatacttcg aaagaaattt attacctttc acaatctgac tttggtattt
    15421 attttagaga aaaattaagt tctcccatgg tttatggaga ggttcctgtt tatgcgaatg
    15481 aagatttagt agtggaatct gggaaattga ctcccaaaac aagttttcaa ataaccgagt
    15541 ggcgcttaaa taaacaagga attccagtat ttaagctatc aaatcatcaa tttatagctg
    15601 cggacaaacg atttttatat gatcaatcag aggtaactcc aacaataaaa aaagtatggt
    15661 tagaatctga ctttaaactg tacaatagtc cttatgattt aaaagaagtg aaatcatcct
    15721 tatcagctta ttcgcaagta tcaatcgaca agaccatgtt tgtagaagga agagaatttc
    15781 tacatattga tcaggctgga tgggtagcta aagaatcaac ttctgaagaa gataatcgga
    15841 tgagtaaagt tcaagaaatg ttatctgaaa aatatcagaa agattctttc tctatttatg
    15901 ttaagcaact gactactgga aaagaagctg gtatcaatca agatgaaaag atgtatgcag
    15961 ccagcgtttt gaaactctct tatctctatt atacgcaaga aaaaataaat gagggtcttt
    16021 atcagttaga tacgactgta aaatacgtat ctgcagtcaa tgattttcca ggttcttata
    16081 aaccagaggg aagtggtagt cttcctaaaa aagaagataa taaagaatat tctttaaagg
    16141 atttaattac gaaagtatca aaagaatctg ataatgtagc tcataatcta ttgggatatt
    16201 acatttcaaa ccaatctgat gccacattca aatccaagat gtctgccatt atgggagatg
    16261 attgggatcc aaaagaaaaa ttgatttctt ctaagatggc cgggaagttt atggaagcta
    16321 tttataatca aaatggattt gtgctagagt ctttgactaa aacagatttt gatagtcagc
    16381 gaattgccaa aggtgtttct gttaaagtag ctcataaaat tggagatgcg gatgaattta
    16441 agcatgatac gggtgttgtc tatgcagatt ctccatttat tctttctatt ttcactaaga
    16501 attctgatta tgatacgatt tctaagatag ccaaggatgt ttatgaggtt ctaaaatgag
    16561 ggaaccagat tttttaaatc attttctcaa gaagggatat ttcaaaaagc atgctaaggc
    16621 ggttctagct ctttctggtg gattagattc catgtttcta tttaaggtat tgtctactta
    16681 tcaaaaagag ttagagattg aattgattct agctcatgtg aatcataagc agagaattga
    16741 atcagattgg gaagaaaagg aattaaggaa gttggctgct gaagcagagc ttcctattta
    16801 tatcagcaat ttttcaggag aattttcaga agcgcgtgca cgaaattttc gttatgattt
    16861 ttttcaagag gtcatgaaaa agacaggtgc gacagcttta gtcactgccc accatgctga
    16921 tgatcaggtg gaaacgattt ttatgcgctt gattcgagga actcgcttgc gctatctatc
    16981 aggaattaag gagaagcaag tagtcggaga gatagaaatc attcgtccct tcttgcattt
    17041 tcagaaaaaa gactttccat caatttttca ctttgaagat acatcaaatc aggagaatca
    17101 ttattttcga aatcgtattc gaaattctta cttaccagaa ttggaaaaag aaaatcctcg
    17161 atttagggat gcaatcttag gcattggcaa tgaaatttta gattatgatt tggcaatagc
    17221 tgaattatct aacaatatta atgtggaaga tttacagcag ttattttctt actctgagtc
    17281 tacacaaaga gttttacttc aaacttatct gaatcgtttt ccagatttga atcttacaaa
    17341 agctcagttt gctgaagttc agcagatttt aaaatctaaa agccagtatc gtcatccgat
    17401 taaaaatggc tatgaattga taaaagagta ccaacagttt cagatttgta aaatcagtcc
    17461 gcaggctgat gaaaaggaag atgaacttgt gttacactat caaaatcagg tagcttatca
    17521 aggatattta ttttcttttg gacttccatt agaaggtgaa ttaattcaac aaatacctgt
    17581 ttcacgtgaa acatccatac acattcgtca tcgaaaaaca ggagatgttt tgattaaaaa
    17641 tgggcataga aaaaaactca gacgtttatt tattgatttg aaaatcccta tggaaaagag
    17701 aaactctgct cttattattg agcaatttgg tgaaattgtc tcaattttgg gaattgcgac
    17761 caataatttg agtaaaaaaa cgaaaaatga tataatgaac actgtacttt atatagaaaa
    17821 aatagatagg taaaaaatgt tagaaaacga tattaaaaaa gtcctcgttt cacacgatga
    17881 aattacagaa gcagctaaaa aactaggtgc tcaattaact aaagactatg caggaaaaaa
    17941 tccaatctta gttgggattt taaaaggatc tattcctttt atggctgaat tggtcaaaca
    18001 tattgataca catattgaaa tggacttcat gatggtttct agctaccatg gtggaacagc
    18061 aagtagtggt gttatcaata ttaaacaaga tgtgactcaa gatatcaaag gaagacatgt
    18121 tctatttgta gaagatatca ttgatacagg tcaaactttg aagaatttgc gagatatgtt
    18181 taaagaaaga gaagcagctt ctgttaaaat tgcaaccttg ttggataaac cagaaggacg
    18241 tgttgtagaa attgaggcag actatacttg ctttactatc ccaaatgagt ttgtagtagg
    18301 ttatggttta gactacaaag aaaattatcg taatcttcct tatattggag tattgaaaga
    18361 ggaagtgtat tcaaattaga aagaataatc tttaatgaaa aaacaaaata atggtttaat
    18421 taaaaatcct tttctatggt tattatttat ctttttcctt gtgacaggat tccagtattt
    18481 ctattctggg aataactcag gaggaagtca gcaaatcaac tatactgagt tggtacaaga
    18541 aattaccgat ggtaatgtaa aagaattaac ttaccaacca aatggtagtg ttatcgaagt
    18601 ttctggtgtc tataaaaatc ctaaaacaag taaagaagaa acaggtattc agtttttcac
    18661 gccatctgtt actaaggtag agaaatttac cagcactatt cttcctgcag atactaccgt
    18721 atcagaattg caaaaacttg ctactgacca taaagcagaa gtaactgtta agcatgaaag
    18781 ttcaagtggt atatggatta atctactcgt atccattgtg ccatttggaa ttctattctt
    18841 cttcctattc tctatgatgg gaaatatggg aggaggcaat ggccgtaatc caatgagttt
    18901 tggacgtagt aaggctaaag cagcaaataa agaagatatt aaagtaagat tttcagatgt
    18961 tgctggagct gaggaagaaa aacaagaact agttgaagtt gttgagttct taaaagatcc
    19021 aaaacgattc acaaaacttg gagcccgtat tccagcaggt gttcttttgg agggacctcc
    19081 ggggacaggt aaaactttgc ttgctaaggc agtcgctgga gaagcaggtg ttccattctt
    19141 tagtatctca ggttctgact ttgtagaaat gtttgtcgga gttggagcta gtcgtgttcg
    19201 ctctcttttt gaggatgcca aaaaagcagc accagctatc atctttatcg atgaaattga
    19261 tgctgttgga cgtcaacgtg gagtcggtct cggcggaggt aatgacgaac gtgaacaaac
    19321 cttgaaccaa cttttgattg agatggatgg ttttgaggga aatgaaggga ttatcgtcat
    19381 cgctgcgaca aaccgttcag atgtacttga ccctgccctt ttgcgtccag gacgttttga
    19441 tagaaaagta ttggttggtc gtcctgatgt taaaggtcgt gaagcaatct tgaaagttca
    19501 cgctaagaat aagcctttag cagaagatgt tgatttgaaa ttagtggctc aacaaactcc
    19561 aggctttgtt ggtgctgatt tagagaatgt cttgaatgaa gcagctttag ttgctgctcg
    19621 tcgcaataaa tcgataattg atgcttcaga tattgatgaa gcagaagata gagttattgc
    19681 tggaccttct aagaaagata agacagtttc acaaaaagaa cgagaattgg ttgcttacca
    19741 tgaggcagga cataccattg ttggtctagt cttgtcgaat gctcgcgttg tccataaggt
    19801 tacaattgta ccacgcggcc gtgcaggcgg atacatgatt gcacttccta aagaggatca
    19861 aatgcttcta tctaaagaag atatgaaaga gcaattggct ggcttaatgg gtggacgtgt
    19921 agctgaagaa attatcttta atgtccaaac cacaggagct tcaaacgact ttgaacaagc
    19981 gacacaaatg gcacgtgcaa tggttacaga gtacggtatg agtgaaaaac ttggcccagt
    20041 acaatatgaa ggaaaccatg ctatgcttgg tgcacagagt cctcaaaaat caatttcaga
    20101 acaaacagct tatgaaattg atgaagaggt tcgttcatta ttaaatgagg cacgaaataa
    20161 agctgctgaa attattcagt caaatcgtga aactcacaag ttaattgcag aagcattatt
    20221 gaaatacgaa acattggata gtacacaaat taaagctctt tacgaaacag gaaagatgcc
    20281 tgaagcagta gaagaggaat ctcatgcact atcctatgat gaagtaaagt caaaaatgaa
    20341 tgacgaaaaa taaccctgag agaggctgga gc
//
LOCUS       DAVHEW010000030        20259 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVHEW010000030 DAVHEW010000000
VERSION     DAVHEW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.030"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(147..1127)
                     /locus_tag="VVL53_000980"
     CDS             complement(147..1127)
                     /locus_tag="VVL53_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU4183170.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1465..1614)
                     /locus_tag="VVL53_000981"
     CDS             complement(1465..1614)
                     /locus_tag="VVL53_000981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183171.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHRFEVADRTDEVS
                     NIYIR"
     gene            complement(1642..3657)
                     /gene="recG"
                     /locus_tag="VVL53_000982"
     CDS             complement(1642..3657)
                     /gene="recG"
                     /locus_tag="VVL53_000982"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU4183172.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKYLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3676..4779)
                     /locus_tag="VVL53_000983"
     CDS             complement(3676..4779)
                     /locus_tag="VVL53_000983"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU4183173.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4769..5137)
                     /locus_tag="VVL53_000984"
     CDS             complement(4769..5137)
                     /locus_tag="VVL53_000984"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU4183174.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5176..6207)
                     /locus_tag="VVL53_000985"
     CDS             complement(5176..6207)
                     /locus_tag="VVL53_000985"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4183175.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6209..7240)
                     /locus_tag="VVL53_000986"
     CDS             complement(6209..7240)
                     /locus_tag="VVL53_000986"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4183176.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7321..9834)
                     /gene="secA"
                     /locus_tag="VVL53_000987"
     CDS             complement(7321..9834)
                     /gene="secA"
                     /locus_tag="VVL53_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU4183177.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9980..10267)
                     /locus_tag="VVL53_000988"
     CDS             complement(9980..10267)
                     /locus_tag="VVL53_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183178.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10295..10927)
                     /locus_tag="VVL53_000989"
     CDS             complement(10295..10927)
                     /locus_tag="VVL53_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4183179.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10931..11572)
                     /locus_tag="VVL53_000990"
     CDS             complement(10931..11572)
                     /locus_tag="VVL53_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183180.1"
                     /translation="MFKNFNNILLNRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11565..12356)
                     /locus_tag="VVL53_000991"
     CDS             complement(11565..12356)
                     /locus_tag="VVL53_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183181.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     VKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12541..12876)
                     /locus_tag="VVL53_000992"
     CDS             complement(12541..12876)
                     /locus_tag="VVL53_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183182.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(13016..13351)
                     /locus_tag="VVL53_000993"
     CDS             complement(13016..13351)
                     /locus_tag="VVL53_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183183.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13440..14750)
                     /gene="der"
                     /locus_tag="VVL53_000994"
     CDS             complement(13440..14750)
                     /gene="der"
                     /locus_tag="VVL53_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU4183184.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14764..15477)
                     /locus_tag="VVL53_000995"
     CDS             complement(14764..15477)
                     /locus_tag="VVL53_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU4183185.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15474..16370)
                     /gene="dnaI"
                     /locus_tag="VVL53_000996"
     CDS             complement(15474..16370)
                     /gene="dnaI"
                     /locus_tag="VVL53_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU4183186.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPKEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16371..17540)
                     /locus_tag="VVL53_000997"
     CDS             complement(16371..17540)
                     /locus_tag="VVL53_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU4183187.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17541..18014)
                     /gene="nrdR"
                     /locus_tag="VVL53_000998"
     CDS             complement(17541..18014)
                     /gene="nrdR"
                     /locus_tag="VVL53_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU4183188.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18154..18519
                     /locus_tag="VVL53_000999"
     CDS             18154..18519
                     /locus_tag="VVL53_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4183189.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18524..19219
                     /locus_tag="VVL53_001000"
     CDS             18524..19219
                     /locus_tag="VVL53_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183190.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19236..20003
                     /locus_tag="VVL53_001001"
     CDS             19236..20003
                     /locus_tag="VVL53_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183191.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
BASE COUNT         5912 a         4563 c         3797 g         5987 t
ORIGIN      
        1 tcgatttgac tgtcctgatc gatttgtcct gttcttattt cattttacta tatttccatg
       61 ataaaacgca tagtataaag ttttcttaat cccctgatac tatgcgtgtt tgtaattttt
      121 aagattttgt gcttagagtc gactccttat tttagatatt taaaaggaat ctcacttcca
      181 cagagccagt tgtagacttg gtcattgaca aatacattca tggcttcgtg agcatactca
      241 ggcatgatgc gataggtttt atcgcaggtc agacgattat aaatcgcaaa ctgggtaatg
      301 ggatagcaaa catcgtcgtc caagcccgta atcatcttaa cctcaccttg gatacgatgg
      361 gcaagatttt tgacatcgat ataggcaagg gtcgccatga tttcctcctc tgtttcatgg
      421 aaggggtcgt gaaacttgaa ataacggaaa agttcgtcgt aagcctcgct agtattacca
      481 atctcaatca cccgtctgaa gtctgacaag aagggataaa tggcaactgt tttctgaatt
      541 cgaggattga gcgctgctgc aactagagct agagcccctc cttgtgaggc accatagcta
      601 gaaagacgct tctcatcaac ctgagacaga ctagcaacaa tttcgaccaa ctggtaaata
      661 tccagataaa catccttata aaagaggtgg tcccgacctt ccacagcacc acggataata
      721 tgccccttca cggtatttcc taaaggagaa cgcaagccgt cttgtgagta acctgactgg
      781 ccccgcacat ccatggaaac aacaccgtaa ccagctacgg tgaagcccag catgtcggcc
      841 cagtcccagc cacgtcccat ataaccatga aaatggaaga ttaatgggac cttctcctca
      901 ctctttggaa gaacaatgcg tgcatagacc tttccttcct tgcttccttc aaatgttaac
      961 tcatagcact tgacttgagg aatgtggaaa tctctttcct ccaagtggta ggatggaagc
     1021 gtggaaacat ttttcacttc cccatcccag aaatcatcaa agtcttccgg aacctcatcc
     1081 cttcctctat aggtcttaat ttcttctagc aaagctggat ttttcatagc atgctccttt
     1141 tattttgtaa tcgttaccac aactgtagca tatctatgta atcaatgcaa gaaagaaggg
     1201 taaatagaaa gtgaatttag attctttact tttaggatga tgggctgaaa gtagttttag
     1261 aaacgaaaaa agagcatttc gctctctctt ctgtttatac tagttttttg cttccttctt
     1321 ttggaaagtg gtttggtaat ttactttaat cgttactgtc atatgagagt cctcgtttct
     1381 ttttgatgac tctagtataa gagccaaacc taaaaactag cttaatactc ttcgaaaatc
     1441 tcttcaaacc gcgtcagcgt cgccttaccg tatatatatg ttactgactt cgtcagttct
     1501 atctgccacc tcaaaacggt gttttgagct gacttcgtca gtttcatcca caacctcaaa
     1561 acagtgtttt gagcaacctg cggttagctt cctagtttgc tctttgattt tcattgagta
     1621 taagttttcc ttagagaaag cttaatccag atgttctttc ttttccagat gaagggcaat
     1681 catgcgccac tctggatctt cttgccaagc ttctatagaa ctaatgtagc tagcaacctt
     1741 tcttgcttct tctaaaatcg gaaaatcttc gataatatca gccacttgga actctggaag
     1801 tcctgactgt ctggttccaa aaatctcacc agaaccacgc attttcaaat cttcctccgc
     1861 aaggacaaat ccattggtcg tttctgtcat gatacgcatg cggtctttcc cagaatccgt
     1921 cttgggatta gcaacgagaa cagcgtagga ctgcttgtcc ccccgaccga cacgacctct
     1981 aagctggtga agttgactga gaccgaagcg atcggcatcc atgataatca tgacagtcgc
     2041 attgggaacg ttgaccccaa cctcaataac cgtcgtcgaa accagaatat ccgtctttct
     2101 ctccttgaaa tcctgcatga tctggtcttt ttcgtcactc ttcatcctac catgtagaag
     2161 agccacctct gccttgcctg caaaatgagt cgtcaactcc tctgataagg caatggcatt
     2221 tttcaaatct agagcttctg attcttcaat caaaggagag atgacataga cttgggaacc
     2281 tttttgaatt tccccctcta accaagtcaa gacctgaggt agttgctcat gtttgatcca
     2341 gcgcgtcaca ataggcttcc gacctgctgg catctggtcg ataatggaaa catccatatc
     2401 tccaaaggct gtgatggcaa gcgtccgtgg aatgggagtc gccgtcatca tgaggacatc
     2461 tggattgtca cctttttccc gtaaaatacg cctttgccct acaccaaaac ggtgctgctc
     2521 atcgataata atcaaaccaa gacgagcata ctccacccca tcttgtatca gagcgtgagt
     2581 tcctataatc aaatcagcct cacccttggc aatggtctcc aagacttctc tcttttctgc
     2641 agctttcaag gaacctgtca agagagccag tttcaaattg ggaaaaaggt tctgtaaact
     2701 ctcaaagtgt tgctctgcga ggatttctgt tggtaccatt agggcagcct gataacctgc
     2761 tgtcactgcc gcaaacatgg ccaagccagc gactaccgtt tttccactcc ccacatcccc
     2821 ttgtaggaga cgattcatgt ggtggtcgga cttcatatca gttaaaattt cctgcaaact
     2881 cttttcctga gcttgggtca gggcaaaagg aagacttact ttaactgctg tcactttttc
     2941 ctgagaccaa ttcagaacca gaccacttcc ctgaactcta ttttcagact tgagcatctg
     3001 cagctgcatt tggaaataaa agagttcctc aaactttata cggcgaagag cctgcttgta
     3061 ttctgccaaa tactttggaa aatgcatagc acggactgcc tgacaacggg acatgagttt
     3121 gtatttgtct agtaaagact ggggcagatt ttcttctatc aagaggtcca gtccctgatc
     3181 aaaagccgtc ttgatgacct tgaccagact ggcctgactg attccctgag ccagacgata
     3241 gacaggctgg aggtcatctt ctacctgagc cagaaccttc atcccagtca gactagcctt
     3301 agcgcggtcc cattttccaa agacagcaag ggttgctccc aactctattt tatcagccag
     3361 atagggctgg ttaaagaaat tcaccgcaaa aacgacctct ccctgcttga gactaaaacg
     3421 caggcgattg cgcttgaaac cataatactg gacactagca ggagtcacta cctgaccaga
     3481 aagaactgcc ttctcaccgt cttccagctc cagcacctgc ttggttttga agtcttcata
     3541 acggaaagga aagtagagca agagatcttg caagttttca attcctagtt tggcgtattt
     3601 ttctgctgac tttggtccca caccaggcaa gacatgcaag ggttgatgta gattcatgct
     3661 ccactccttt cttttctaat aatattctct cggaatacgg tcgctgagga ggcaaaccac
     3721 ctcatagtta atggttacgc ggtaggtcgc tacctgagtt gcagtgattt ccttatcccc
     3781 attggagcca atcaaggtta ccttggttcc tagcggataa agcttaggca atcgaatagt
     3841 gatttggtcc atcgaaaccc tgccgacaat tgggcaagct tggccatcta ccaagacaga
     3901 gaaattttgc atgtctcttg tccatccatc tgcatacccg attggcacgg tcgcgatgac
     3961 ttgctcgcta tccgcttgat aagttgctcc atagcccatg caagctccag ctggaactgt
     4021 cttgacatga accagagcag actccaaggt caaggccggt atcaaatcat aaggcaaatc
     4081 caagaccgct ccacttggat tgaggccata catggcatct cccatacgaa ccgcattgaa
     4141 aatagtctct acatgccaaa gagtcgttgc agaattgcta gcatgaacca gctctggaac
     4201 ttccttcata ctagctaaaa tagttttaaa ccgttctaac tgggcattaa aatagtcatc
     4261 tgattcctca tcagcagtag caaagtgggt aaagattcct tcaacacaaa caccgtgttg
     4321 ttggagcaaa tcttgagcct gctcaacctc acttgcctct ctaaaaccaa tccgtcccat
     4381 ccctgaatca atcttgaggt ggactgtcaa tccagttagg tccacttcct tatctaagag
     4441 tgcttgaatc cactccagtc cagccactgt caaggtgaag tcatattctt tagctagagc
     4501 aacagcttcg atttcagaaa ctcctaaaat gaggattggc ttgctgagtc cagcttgtct
     4561 gagttcaatg gcttcatcga tattggaaac gcaaaagcca tcaacatcat cttgaattgc
     4621 cttggcaacg gcaacagctc catgaccata agcattggcc ttaaccacag ccaacttgag
     4681 cgttccttga gggatatgag cccccatttg ctgaatattt tgtcgaatag ctcccagatg
     4741 aatcagagcc ttggttggtc tatgtggact agctttcatg attttcctcc aaaatgacac
     4801 tggctgtcac aaactgatcg gtgtggctga tagacagcca aatctttcct gaaaatggtg
     4861 cctgactaaa ataaggcgcc ccacgttcat tgttcaagac ttccaaatcc tgaaaaccga
     4921 gcttgctaat gcccgttccc atagccttgg aaaaggcctc cttagccgac cagcgaccag
     4981 ctaaatattc tatttgcctg cgtcctttga gactggtgaa gcgctccatt tcctgagcgg
     5041 tcagtacacg cttagcaaat ccttcatgtc gtgtaactgc gctttctatc gaagccaatt
     5101 cttcgatgtc aattccgtgt ccaactatca ttctcatcaa aaggagttga gattccccaa
     5161 ctccatcctt ttcacttatt ttgtcaaggt aacataaatc tcttctacca aggcctctgt
     5221 attttcccaa cctaggcaag ggtcagtaat agagcaacca aagatctctg gttggttttg
     5281 acgaccatct gctaggtaag attcaatcat aaatcctcga accgtctttt taattttctc
     5341 attccaatca cgattctgca aggtctggcg aacaattcga atctgctcca tatattgctt
     5401 gcctgagtta tcatggttgg tgtcaatgag gataaaagga ttttcaagtc ccatggtttc
     5461 atagcgttca atggcttgga gtagattttc atagtagtaa ttcggcatat aattgccata
     5521 ctcgttgact gctccacgga ggataacatg ggccaaagga ttacctgatg tctcaacttc
     5581 ctgcccatga taaaggaagg tttgcttgtt ttgagcagca tagatggcgt taaacataac
     5641 acccaaattt cctgaggttg gatttttcat ccctactggt gcatcaatcc cagaagccac
     5701 aaagcggtgc tcttggtctt ccacagaacg agctccaacg gcatggtagc tgaccaagtc
     5761 atccaccaag atcagatttg acggataaag catctcatct gccgttgtca aaccagtctc
     5821 tgtaatcacg cggtagtgca actggcgcac agcctgcaag ccattaatca ggcttggagc
     5881 cttagaagta tctggctggt gaactaatcc tttatagccg tctccattgg tacgaggctt
     5941 agcagtatac acgcgcatga ccatgaaaat cttatccgct accttctttt gcaaggcaga
     6001 taaacggcga gcatattcca agaccgcctc ttcattatca gaagagcaag gaccaatcac
     6061 caagagaata cggtcatctt cccctgaaat aatatctgcc aattccctgt cacggctttc
     6121 cttgagtctc aaggcttccg cagacaattg ggtttcagcc ttgatgactt ccatatcgat
     6181 ttcttgacct ttttcaataa atgccatctt attctcctaa cgttttgtaa atttctctga
     6241 caagagcttc tgtgttatcc caacccaggc aagggtctgt gatagactta ccaaatactt
     6301 ctggctcatt ttgtcgacca tcttccagat aagactcaat cataaaacca cgaacgaact
     6361 gcttaatttt ttcattccaa gcacggttaa tcaaggtctg gcggacaatt cggatctgtt
     6421 caatatactg cttaccagaa ttgtcatgat tggtatcaat gatgataaaa ggattttcca
     6481 agcccatttt ctcatactgg gcaatggtat caattaaatt gtcataatag tagttgggaa
     6541 tattttttcc atactcatta agagcaccac gaagaatagc gtgtgaaagc gggttcccag
     6601 ttgtttctac ttcttttcct aagaaaagga aactttgttt gttttgagca gcataaatcc
     6661 cattaaacat gacattgaga tttccagagg ttggattttt aaaaccagta gaaaatcctg
     6721 ccccacttgc cacaaagcgg tgttgctggt cttcaactga acgggcacca actgccatgt
     6781 aagaaatcaa atcatctaca agcggaaggt tttcaggata aagcatttca tcagcagttg
     6841 tcatccctgt ttctgtgatg acacgatagt gaagatggcg aacggctttg attccattga
     6901 taagactagg cgcttctgtc gcgttaggct ggtgaatcaa gcccttatag ccatctccgt
     6961 tggtacgggg tttggcagta taaacacgca taaccataaa gatacgatct gccacttctt
     7021 cttgtaggac tgccaaacgc ttagcgtatt caaggacagc ttcttcgttg tcagatgagc
     7081 atggcccgat taccaagaga attcgctggt cttctccacg tataatggct tctagctctt
     7141 gatctcgctg tgatttcctc tccaaagcct gaccttctaa ttttgacaag gcacgaactt
     7201 cttctatatt tattttagag ctttttgctg taaataccat aactcatctc cttataaagc
     7261 aaacggatac ccagtaaaaa tttacttttc acaagatatc cgcctctaaa ctatctcatt
     7321 ttattgtctt ttaccgtgac agtttttaaa tttcttacca gaaccacatg ggcaaagttc
     7381 attgcgtcca atctggctca aatccaaatc ttctggcata cttgcttggt gagcagcgat
     7441 attgcgagtc gctgttgtac tgatatgacg ttctgcctgt ggtctttctt gttcatgaat
     7501 ttgtgctttc atcatcaagc gtgtcacatc aaactcaatc gaaccaatca tatcattaaa
     7561 catacggaaa ccttctgcct gatactcaac aacagggttg ttctgagcat agccacgaag
     7621 tccaaccgcg ttacgcaatt gatcaagggc atcgatatga tctgtccact tgttatccac
     7681 cactcgtaga atcaaaactt tttggaattc tttaactgct tcttcatcgc gtagttttga
     7741 aacctgacta tcgtaaacct tcaaggaacg ttggaaaagc tcttccttga tggccttatc
     7801 agacaagcct gacaagtctt ccatcgtaat agaatcttca ggaagcaagt tgtacttagc
     7861 aaagttcaaa attgcctcta gtttttcatc ttgtttggca cgcgcatgac catcaacgac
     7921 acgttcaatc gtgcgtttga tcatagactg aatttcaggt gccaagtcac gatctgcagt
     7981 gatgacatcg taacgttgag catagataat ctcacgttgt tcacgcatga catcatcgta
     8041 ttgaaggact tgtttacggg tatcgtagtt atttccttcg acacgtttct gagctgcttc
     8101 aacctgacgc gtcaacatgc gagactcaat ggcctcttca gacatgttca agcgttcaaa
     8161 gattcccttc aagcgttcag aaccaaaacg tttcatcaaa tcatcttcaa gagataggta
     8221 gaattgtgac tcacctggat ctccttgacg acctgaacgt ccacgaagct ggttatcgat
     8281 acgacgactt tcatgacgtt ctgtaccaat aacacaaagt cctccaagtt cacgaacacc
     8341 ttcaccaagc ttgatgtcgg taccacgacc cgccatgttg gttgcgatgg taacggcacc
     8401 acgttgacca gcattcatga tgatttgggc ttctctatag tggtttttgg cattcaagac
     8461 ttcgtgagga acaccagctg caaccaattt cttagaaatg tagtcactag tttcaaccgc
     8521 tactgtacca accaagacag gttgaccctt ttggtaacga gccttaacgt cttcgacaac
     8581 cgctttaaac ttagattcga tacttgcata aagaaggtct gagtggtcaa tacgttgaac
     8641 aggacggttt gttgggattg gaataacacg aatgttgtag atttcacgga attcttcttc
     8701 ctcagtctta cctgtacccg tcataccaga caatttcttg tacatacgga agaggttttg
     8761 gtacgtgatt gaggcagatg tcttggtttc atcctggatt ggcacacctt ctttggcttc
     8821 aatagcttgg tgcaatccat cagaataacg acgaccttcc atggtacgac ctgtaaattg
     8881 gtcgacaatc aagatttctt gctcttcgct caccacatag tcaatatcga gaagcatgat
     8941 gtagttggca cgaagggcgt tatcgataaa gtgagtcaaa gccacgtttt cgatgtcata
     9001 gaggttttca agtttgaagt agctttcagc cctgtcaatc cctgaatcag acaaaccaat
     9061 agtcttagac tgcacatcga tgatgtagtc atctttgttc aaagatttta catagtggtc
     9121 tgccatgtga tacaactgac tggtttcaac cgcattggca cctgatacaa tcaaaggtgt
     9181 acgagcctcg tcaatcaaga tagagtcaac ctcatcgacc aaggcatagt taagcggacg
     9241 ttgtaccatg ttttcggcgc gaacgaccat gttgtcacga aggtagtcaa atccgatttc
     9301 tgagttagtt gagtaagtaa tatcacactc ataggcttct tttttctcca ttggagattt
     9361 ggtagccaag ttaatcccta ctgacaaacc aagccaagag tacaattcac ccatctcagt
     9421 cgcgtcacgt tctgacaggt attcattaac cgtaactacg tgaacccctt tacctgaaag
     9481 ggcattgagg tataccggca tggtcgcagt caaggttttc ccttcccctg tacgcatctc
     9541 tggcacgtca ccatggtgaa gaacaatccc ccccatgacc tgaaccttat aagggaagag
     9601 acctaggaca cgtttggcac cttcacggac aaccgcaaat gcttcgtaaa gcaatgaatc
     9661 cagtgattct ccattttgat aacgttcctt aaattcaact gtttttgctt ttagttggtc
     9721 gtcagtcaaa gcagccattt ggtcttcgta tttgaaaacc ttgtcagcca tcttttccag
     9781 acgacggatt tctcctttat cattttcgat aattgttttt aaaatattag ccatgttttt
     9841 ccttacttta aattccgaat attttagaat gttcctttaa ttttagcaca attactgatt
     9901 attttcaagg aagaatcccc attttgaaaa ggaaaaagag gttagttttc aagctaacct
     9961 ctccgacttt tatgactgtt taattgccaa aaatcggcaa aatggcaaat gtgataaata
    10021 agttaatcca aagagaaaga cagcaaatca gtccaaaaac aaagagactg actttcttgg
    10081 acagcaaggc catgagaatg gacagaattc caaaagctag caagattttc tgtatgatga
    10141 agatgtcaat ccttatcccc agaagtggtt tatcccaagg gataaagaag aaagacagcc
    10201 agatcagcag aactaaacca atataaacct tggaatatcg tgtaataaat gattttagat
    10261 gtgccataag ccatctccta ggattgtata tcctttacat ttgtatatgt ccattttcga
    10321 caactaatac atctgtacaa aggtctgaaa tatcttccga tatgtgcgac gtcaggataa
    10381 ccagaccatt ttcttttttc aggtaagata aaatgacctg tttggttaac ctcacactct
    10441 tctcatccaa agcattcata ggttcatcga gaaagagtat agaaggagag gaaataaagg
    10501 cttgaatcaa ggccattttt tgctttgttc ctaaggataa atgacggtat tcaatgtctt
    10561 caaattcctg taaatcatag tattgaatcc aataggcaat tcttttttcc gtaactttag
    10621 atgaaaaata ccttaacagt tccaaatttt ctctcaggga taaatgagat aaaaactcga
    10681 ctttttcaat taaaattcct gcatcctgaa tataatgatt ttttaccccg taaactttac
    10741 catcttgaag aacttttcct ttgtcaagct taatataacc agcaagtatc tttaaaagaa
    10801 ccgtcttgcc agatccatta tcacctttaa gtccataaat ttttcctgat tgaaacacca
    10861 aattcaactg atttaaaata ggtcttttcc cataaatttt ctgtccattt ataatttcaa
    10921 gtctcatcgt ttatcctttc ttaaatttct cttccaagcg actaacaatc aacataacta
    10981 ataaacttaa gagtactaaa tacactgtta caatatgatc catcatccat tcttgtccca
    11041 caaatgctaa aaaatagaga aaaaaggaaa aaccagcgcc cgcactaatc agtgctatca
    11101 gataagacag tagtaagtac aacatcatta actgagcaag taaaaagaaa agtgcgactg
    11161 ttttgaaatg aattcctaga ggaagtaaac caagaaacca gacaaacaaa tcttgagtaa
    11221 aaaatttaga aatgacaacc aatcgtttcc ttgtataatc gaaaaaggag cttgattgat
    11281 agcgaatcaa gtgatggtaa tggtctgcca aatcactttc aagtaaaatg acagagagcc
    11341 atcctaccat gaaaagcgaa aggtttaaca atagtttggg gatttctaaa ttcgcttcag
    11401 aatagtcatt ataggaaaaa attgaaatca attctctctt gaaaaagata acagcatcct
    11461 gcttttgaac cgctgttgac aatagatggt ttatcaaaat catcatcaga actatacgaa
    11521 gtagtaaaac aatctttcta tttagcaaaa tattgttaaa attcttaaac attatcttca
    11581 tatttttcaa gcaccatgta agggacaata taaaggatat aaggagtaaa gagtttcatc
    11641 aaacttacat ccccatagct agcttgcatc aaactcgtca taggaaccat atagtaaggc
    11701 aaggcactat aaagcagcat agaaccaagc caaagaaaca tcaaataggt gattgccgaa
    11761 cgagtcacat tccccacata atcaactatt tgtaaaaaat agattgcatt gatgaaaata
    11821 ccgattagta ggacacaagt aaagaacaaa tagcttttta tctctccatc taggagtctt
    11881 tgtaaaccac ttccatcaga aaatagagaa ttccatccaa gaggagaaaa agtcccaaag
    11941 aaataggtta taattgcaat tatcagcaca gtcactaaat atatcaaaca agggatactt
    12001 gcaacttgca aagtcaactt tctttttaac ccttgataac tcacttctct tccaatactc
    12061 aatcgtaaaa ctttattttt cagctcaata tattgaaaac ctagcaaaac aatgatgatt
    12121 ggaataataa atgattgata aaaacctaga ttgaactgga gaatacgaaa atctaatgct
    12181 gattttaata agtcattttt aattgcttgt ccatcgaatt taactagatc gggcgcatga
    12241 atatcaaaaa agaaataagt catcaaaaca tcacgataaa ccaaagcaag aaaaagcaaa
    12301 gtaagcaaga cataaacaaa tttatgcctt gcaaaagtta agagaggaat cttcataagc
    12361 cacctcctac aaatagaaag taatataata ctcaatgaaa atcaaagagc aaactaggaa
    12421 actagccgca agttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag
    12481 ttacatatat aatccaaggc gacgttgacg tggtttgaag agattttcga agagtataaa
    12541 tcaatgcctt ccgcctttag acttccctag ttcccgtctc aagtcaagca tttttttgaa
    12601 aaagacatag gctaaccagt cactagcaaa agcgataagt agtaataata gccaaatacc
    12661 ccataacttg atatctgtca aatttctcaa gacgatattg aaaaacagaa ctgaaaaaac
    12721 tgtccaagca aggctaaaaa gagcatagag ggtgatgtaa atccagtaaa aatagtaaaa
    12781 aattgggaaa aatttacttt ctctattatc cttgtcaatc cagctgaaaa agtaaaaaca
    12841 gggtaataag attatcattt taaacaagtg ttccatcaca aacctcctct tttaaattat
    12901 tttctttagt ttcgttctat taaatgttat taccaatata aaccttggaa tagcgtgtaa
    12961 taaatgattt tagaggtacc ataagccacc tcctacaaat agaaaccgat ataaatcaat
    13021 gccttccacc cttagacttc cctagttcct gtctcaagcg aaacatttct ttgaaacagg
    13081 aataagttaa ccaattcata ccaatagcta gcagaataaa aagaaaccaa atgccccata
    13141 acttgatatc tgtcacattt ctcaagacgg tattgaaaaa cagaactgaa acaactgtcc
    13201 aagcaaggct aaaaagagaa tagaagggga tgtaaaacca gtaaaaataa taaaaaattg
    13261 gaaaaaactt actatttctg ttggcctttt caatccagtt atcaaaataa aagtacggtg
    13321 ctaaaagtaa gaatttaaac aaatgttcca tcaccgacat ccccccttct tttgatagcg
    13381 ttttctatta ttttattata tcaaaaaaat ccggaactgt cattccagat tctacttttt
    13441 tatttgcgtt ttcttgcgat gagatgaatc ggtgttccct caaaaacaaa ggccttgcgg
    13501 atttgatttt ccaagaaacg caggtaagaa aagtgcatga gttcttcttc attgacaaag
    13561 atgacaaagg ttggtggttt ggttgccact tgggtcgcat agaaaatctt gagacgtttt
    13621 cctttgtctg tcggtgttgg gttgatggca atggcatcca tgatgacatc gttcaagaca
    13681 gctgatggaa tacgtgtatt ttgactttcg ctgatttgct taatcatctc aggaagtttg
    13741 tggagacgtt gcttggttaa agctgataca aagataatcg gtgcgtaagg caggtattgg
    13801 aactgctcac ggatatcttc ttcccagttt ttcatagtgt ggttatcttt ttcaagcgta
    13861 tcccacttgt tgaccacgat aatcatccct ttaccagctt catgggcaaa tcctgcgata
    13921 cgcttgtcgt actcacgaat gccttcttcc gcattgatga ccatcaagac cacatctgaa
    13981 cggtcaatag cacgcatggc acgcataaca gagtatttct cagtattttc ataaacctta
    14041 ccagacttac gcataccagc cgtatcaatc atggtaaact cttgaccatc tgtatctgta
    14101 aagtgggtat caatggcatc acgagttgtt ccagcaacag gactagcaat aacacggtct
    14161 tctcccaaga tagcattgat caagcttgat tttccaacgt taggacgacc aatcaagcta
    14221 aacttaatga catctggatt ttcttcctca tattcatttg gaagattttc tacgatcgca
    14281 tctagcacat cccctgtacc gattccatgg acagatgaga taggcaatgg ttcacccaaa
    14341 ccgagagcat agaaatcata tatatcattt ctcatctcag ggttgtccac cttgttgact
    14401 gcgaggataa ctggtttgtg ggtcttataa agcttacgag ctacgtattc gtctgcatca
    14461 gtaattcctt ccttaccaga cacgacaaaa acgataacat ctgcttcttc catggcaatt
    14521 tctgcctggt gcttgatttg ttccatgaaa ggagcatcga catcatcaat tcctcctgta
    14581 tcaatcatgc taaaagaacg attgagccac tcacccgttg cataaatacg gtcacgtgtc
    14641 actccttcga catcttctac aatggagatt cgctcaccag cgatccgatt aaatagggtt
    14701 gatttcccaa cattgggacg tcctacaatg gcaatagttg gtagggccat aatttctcac
    14761 tttctacaat aatttcttct gttcaagatt ttttctagtt gagcttggtt cagcttgacc
    14821 aaactgttct gctaggcgct gactccagct tgtggtcgca cgcgccccag catagtcagc
    14881 ctgaacacgg tcataagctt ggattgcctc agttgactgt tcttggtatt cttcctcaaa
    14941 gacaacattc tctagtggca gtctcggttt catatcatga tgttgatttg gcacacccag
    15001 tgccatccca aagacagaat aggtgtagtc aggtaggtta aagagctctg ccacttcttc
    15061 agacttgtat cgaaccaaac cgataatcac accaccatag cccaagcttt cagctgccaa
    15121 caaggcgttt tgtccagcaa gagctgcatc gaccgaacta atcaagagac cttccacacc
    15181 ttggggttgg aaggtgtcgg tatgaagtcg ggctcccttt tctgctcggt tcaaatctcc
    15241 gacaaagaga aggaaaacag cagactggcg aatggcttct tgaggtacca attcatacaa
    15301 ggcatctttc ttctcttgac ttcgtaccac aatcacagag taggattgga aattcttcca
    15361 agatgatgcc atctgggctg ctgtcaaaat ctcatttaag tctacttggg gaatttcttg
    15421 ctctttaaac ctgcgcactg aagtatgagc cttcatcaat ttaatggttt ctgtcatcga
    15481 cggtttactc cttctaaacg agtctcctca gccaaataac ggatgcgttc catgacccgt
    15541 ctggcttccc aggtttcgtc atttccatgt ttcactttcg caaaatgctt ctccaaatct
    15601 tcaaagttga agttggatgt gaaaaaggtc ggtaaatttt cctgcatccg atattggaga
    15661 atgacctgca ggatttcgtc acgcacccaa acggttgatt gctcggcgcc aatatcatct
    15721 aaaatcagga cctcagacag cttaatctca tccaccaagg tcttaacatt gccatcactg
    15781 atagcatttt tgacatcaat gacaaagcta ggatagtgga ggagagttga tgaaacacca
    15841 cgtttttctg ataaatcatg agctaaggcc gccaccatga aacttttacc cacaccaaag
    15901 tctccatata agtaaagacc ttttcgaata gctggatatt gctccacgaa ggctagtagc
    15961 ttttcaaaaa ctggtaagcg ccccaaatca tccaagtcaa cttgagccaa actagctttc
    16021 ttgagactgg ctggtagatt gattaacttg agacggttct taatagccgc ttctttttca
    16081 gccgcgatta gctcaggagt ttcttcatat gaaacatctg cataaccatg attcttaacc
    16141 aaaatcggct tgtagccttt ggcaatataa tccgtatccc cacggagaaa cttgtcacgc
    16201 tcggtgatgt actgattaaa cttggagata ctgcgattta attcctttgg agttaaggat
    16261 tcttgctgga taaaggccgc aacatcaggg tccttcatga ttttctggac caaatcttga
    16321 taataaaaac ggctgggttg acgtttgagt acgtctccga cactttccat ctaatctcct
    16381 cctttttcta atcgagctaa tagttcttgc ttcttacgtt ctagttccag acgagtttcc
    16441 tcgctggttt cattcttata ttcaggatta ctccatttag gaacattggt tttttctggg
    16501 gcagtctgat tctgtttttg tgtttttgct ttctgccctc gatcacgaat tcgtaaaacg
    16561 gcctcttctg ccgaatgaat cttttgatag gcatagtcat tggctacctt catggcatat
    16621 ttctcattga tatttgccga atccacctta ttaaaggtca ataagagaat aatattgatg
    16681 acttcgtcca gtaagcccaa gccagccatc tgttgcaaga gttctctttc tgtttgggta
    16741 atggttccct tgcgtgtttg cttgatttct gctaagaact gcagggcagt tttactttta
    16801 gcttctttga taatggtcgc ttccttaaga ctaaagtcag aggaaactgg tttttgagca
    16861 attttttcac gcatgcgttt ggttgaaata acctgggaaa cagctgttga cttggccaat
    16921 tgataggttt caaaccaagt ccatttcttc tcctcggcaa tagcaaagag gtttaagaca
    16981 tcggactgct catccgcaaa acgaagtcca tctcgagcca tcagctggcg aaaatgttcc
    17041 aagtcaaaat cattggccac tttcttcttg agaccaaggt cttcttgact gcctagttct
    17101 gccaattctg gaaagacttg attgagtgag acaggtattt cttcaccatc agcactttca
    17161 actttcaaat cctccacagc tacatcgcca atctttttct ctaagagtct gcgataaaca
    17221 ggatgcccca agaagtcttg actagataga ggagcatgga gggctagctg ataaacatca
    17281 cccttttgat agagggtcaa gagattaaaa gcagataaga ttttcaatga ttttatcagt
    17341 ctatccatcc caaagttgag atggttgaga atgcttgaaa aaagatattc ctttctacca
    17401 ttatcccaaa aactgattgt ataaagataa aggctcagtg cctcctgacc gataatcggg
    17461 aggtagcact gtaccagaga tgaggtatct tgcgacaccc gattattctt tagataagaa
    17521 aaacggtcaa ttggcttcat ttatctttcc tttttctttt tagaggactg ggtgatttgt
    17581 tggagcaagc tctctaactc actgacatcc ttaaaactac gatagacact agcaaaacgt
    17641 acataggtaa tctcgtccaa ttcagccaac tcctccatga cgagtgaacc aatgtcctca
    17701 ctttgaattt cattttcatt tcgaccacgg agtttctgtt cgatacgatt gactaccatg
    17761 ttgatttcat cacttgacac aggacgtttc tgggctgagc ggataatccc attaaagatt
    17821 ttatctctgg agaattgttc ccgtgtgcca tcttttttaa caaccactaa ggttctttct
    17881 tctactcgtt cgtaggttgt aaaacggtgt tggcattcgt cgcactcacg tcttctacga
    17941 atggtgttcc cttcttctgc ttggcgacta tcgataacac ttgacttggt agccccacat
    18001 tttggacaac gcatcctttc cctccttatc gttttctttt cattatacca ttttttaagc
    18061 gattcccaaa acaattcttc tttttgcttg acaagttttt tgttttgttg tattatttaa
    18121 ttaagacaac aaggtaaaag aaaggagact aagatgtcct ggacatttga caacaaaaaa
    18181 cccatctatt tacagattat ggagaaaatc aagcttcaga ttgtttccca tacactggag
    18241 cccaatcaac aacttccaac cgtgagggag ctagctagcg aggctggtgt caatcccaat
    18301 accatccaaa gagccttatc agaccttgaa cgagaaggat ttgtctacag caagcgaaca
    18361 actggacgat ttgtgactaa ggataaggag ctaatcgccc agtcacgcaa acaattatca
    18421 gaagaagaat tggaacactt cgtttcctcc atgacccatt ttggctatga aaaagaagaa
    18481 ctaccaggcg tagtcagtga ttatattaaa ggagtttaag cctatgtcat tactagtatt
    18541 tgaaaatgta tccaaatcat atggagcaac accagccctt gaaaatgttt ctcttgacat
    18601 tccagctgga aaaattgtcg gccttcttgg gccaaacggc tcaggaaaaa caaccctgat
    18661 taaactaatt aatggcctct tacaaccaga tcaaggacgt gtcctcatca acgacatgga
    18721 cccaagccca gcaaccaagg ccgttgtagc ttatttgcct gatacgacct atctcaatga
    18781 gcaaatgaag gtcaaagaag ccctaaccta cttcaagacc ttctataaag atttcaatct
    18841 tgaacgcgcc catcatctac ttgcagacct gggcattgat gaaaatagtc gtctcaagaa
    18901 actatcaaaa ggaaacaaag aaaaggttca actgattttg gttatgagcc gtgatgctcg
    18961 tctctatgtt ttggacgaac ccattggtgg ggtggatcca gcagcccgtg cttatatcct
    19021 caataccatt atcaacaact actcaccaac ttctaccgtt ttgatttcta cccacttgat
    19081 ttctgatatc gagccaatct tggatgaaat tgtcttccta aaagacggaa aagtcgtccg
    19141 tcaaggaaat gtagatgata ttcgctacga gtcaggtgaa tccattgacc aactcttccg
    19201 tcaggaattt aaggcctaag caaaggagat tatttatgtt ttggaattta gttcgctacg
    19261 aatttaaaaa tgttaacaag tggtatttag ccctctacgc agccgtgcta gtcctttctg
    19321 ccctcatcgg aatacagaca caaggcttta aaaatctacc ttaccaagaa agtcaggcta
    19381 ctatgctact ttttctagct acagtctttg gtggcttgat gcttacactt gggatttcaa
    19441 ccattttctt gattattaaa cgcttcaaag gtagtgtcta cgaccgacaa ggctatctga
    19501 ctttgacctt gccagtttct gaacaccata tcatcacagc caaactaatc ggtgccttta
    19561 tctggtcatt gattagcacc gctgtattgg ctctaagtgc tgttattatt ctggctttaa
    19621 cagctccaga atggattcct ctttcttatg tgattacatt tgtagaaaca catctccctc
    19681 agatctttct tacaggtata tccttcctac taaatactat ttcaggaatc ctctgcatct
    19741 acctggctat ttccattgga cagcttttca atgaataccg tacagcactc gctgttgcag
    19801 tctacattgg tatccaaatc gtcattggat ttattgaact tttcttcaat cttagttcta
    19861 atttctatgt caattcactg gtaggactca atgaccattt ctatatggga gcaggtatag
    19921 ccattgttga agaactcata ttcatagcta tcttttatct cggaacctac tacatcttga
    19981 gaaataaggt taatttgctt taaataattt ttacctagat atgtaacata ctcatagaac
    20041 aaaagagacc aggcaaaaag tctttaaaat tagaaaacgc atagtatcag gtgttgaata
    20101 tgtactgccc cccaaaagtt agattttttc tgtctaactt ttgggggcag ttcataagaa
    20161 ccttggtaat atgcgttttt tgtgagctga cttatttcct ttcactatat cgcaaaatga
    20221 aataagaacg gaacgatggg attttggaat tcaaatcaa
//
LOCUS       DAVHEW010000031        19305 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVHEW010000031 DAVHEW010000000
VERSION     DAVHEW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.031"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(35..496)
                     /locus_tag="VVL53_001002"
     CDS             complement(35..496)
                     /locus_tag="VVL53_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4183192.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(463..606)
                     /locus_tag="VVL53_001003"
     CDS             complement(463..606)
                     /locus_tag="VVL53_001003"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU4183193.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            875..1255
                     /locus_tag="VVL53_001004"
     CDS             875..1255
                     /locus_tag="VVL53_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4183194.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1354..1575
                     /locus_tag="VVL53_001005"
     CDS             1354..1575
                     /locus_tag="VVL53_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU4183195.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1592..1906
                     /gene="trxA"
                     /locus_tag="VVL53_001006"
     CDS             1592..1906
                     /gene="trxA"
                     /locus_tag="VVL53_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4183196.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2281..2745
                     /gene="queF"
                     /locus_tag="VVL53_001007"
     CDS             2281..2745
                     /gene="queF"
                     /locus_tag="VVL53_001007"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU4183197.1"
                     /translation="MQHYETVEAVTFAYGQRHHLEIQITREIAKEQGIRHHILDMSLL
                     GQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLD
                     PRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR"
     gene            2807..3199
                     /locus_tag="VVL53_001008"
     CDS             2807..3199
                     /locus_tag="VVL53_001008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183198.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDEVSLKHCFEVADRTDEVSLK
                     HCFEVVDRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVS
                     NHTYDKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(3224..3892)
                     /locus_tag="VVL53_001009"
     CDS             complement(3224..3892)
                     /locus_tag="VVL53_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU4183199.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(4003..4794)
                     /locus_tag="VVL53_001010"
     CDS             complement(4003..4794)
                     /locus_tag="VVL53_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU4183200.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AIGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(5041..6837)
                     /gene="pepF"
                     /locus_tag="VVL53_001011"
     CDS             complement(5041..6837)
                     /gene="pepF"
                     /locus_tag="VVL53_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU4183201.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(6848..7591)
                     /locus_tag="VVL53_001012"
     CDS             complement(6848..7591)
                     /locus_tag="VVL53_001012"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU4183202.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGSKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7593..8543)
                     /locus_tag="VVL53_001013"
     CDS             complement(7593..8543)
                     /locus_tag="VVL53_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU4183203.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNMD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(8681..9109)
                     /locus_tag="VVL53_001014"
     CDS             complement(8681..9109)
                     /locus_tag="VVL53_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4183204.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(9111..10178)
                     /locus_tag="VVL53_001015"
     CDS             complement(9111..10178)
                     /locus_tag="VVL53_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4183205.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            complement(10197..10667)
                     /locus_tag="VVL53_001016"
     CDS             complement(10197..10667)
                     /locus_tag="VVL53_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU4183206.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(10958..11410)
                     /locus_tag="VVL53_001017"
     CDS             complement(10958..11410)
                     /locus_tag="VVL53_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU4183207.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(11447..11620)
                     /locus_tag="VVL53_001018"
     CDS             complement(11447..11620)
                     /locus_tag="VVL53_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU4183208.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            complement(11763..11942)
                     /locus_tag="VVL53_001019"
     CDS             complement(11763..11942)
                     /locus_tag="VVL53_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183209.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(12107..12345)
                     /locus_tag="VVL53_001020"
                     /pseudo
     CDS             complement(12107..12345)
                     /locus_tag="VVL53_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12615..13886
                     /locus_tag="VVL53_001021"
     CDS             12615..13886
                     /locus_tag="VVL53_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU4183210.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKASGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            14150..14222
                     /locus_tag="VVL53_001022"
     tRNA            14150..14222
                     /locus_tag="VVL53_001022"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14183..14185,aa:Lys,seq:ctt)
     gene            complement(14432..15751)
                     /locus_tag="VVL53_001023"
     CDS             complement(14432..15751)
                     /locus_tag="VVL53_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU4183211.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKNGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSIVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(15761..17377)
                     /locus_tag="VVL53_001024"
     CDS             complement(15761..17377)
                     /locus_tag="VVL53_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4183212.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            complement(17406..18296)
                     /locus_tag="VVL53_001025"
     CDS             complement(17406..18296)
                     /locus_tag="VVL53_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4183213.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            complement(18307..19224)
                     /locus_tag="VVL53_001026"
     CDS             complement(18307..19224)
                     /locus_tag="VVL53_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4183214.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
BASE COUNT         5786 a         4306 c         3491 g         5722 t
ORIGIN      
        1 gtttacattc ctatttttat gcataattat tctgtcattc tctaatttgt gaatcaatat
       61 atctgattag aaaactacca ttgagttttt gctccacctc attagtccgc tcaaaacgcc
      121 gtccgctaat cttgtcatag actttctccc ctctaggagc ccctgcataa ggacgataga
      181 ggtagcggtt gattcccgtt tcgtcaatat ataggactgg taaatctttt aggttatcca
      241 aaatatcaag aaactcagaa actttctcag gcttttgttc cctaaaactg gtcgtctttt
      301 ttaaaatgac cttaatctgc tttaaaactg cccatacgga gggcaaagca caatcaaaac
      361 gggccgcaat ttcccgtaaa aaagcgtctg gatgagcctc tacaaaggct ttcaattctt
      421 ctaaagggat cttacgcttt ttgacgactc gctttttccg ctctaagttc ccttgctcac
      481 gtctttcttt tcccacgtga agagagttct gacgccgaca tcaaaaactt tggccgcctc
      541 gacatgtctg tgccctcctt tgatggaatc taatgctcct tgtttaaaat cagtactata
      601 tgccatagct ttattagacc atgcttattt taaactaagc aactatagat agagaaatga
      661 aataaaatct gaattcgaat gtgataagga gttagaaatt gaaacaatag gattccccca
      721 ttattcacac aaaaataaaa ccactaaaaa acagagcttg ccagcattct tgaaaagtag
      781 tataattatt gcatgcgcaa tcatcttgtg acgcagagac tgcccgttaa tggactttct
      841 tctattttat gactctaaaa agaaaggaga cactatgacc tatttggaaa aatggtttga
      901 cttcaatcgg cgtcagaaag aaattgaaag tctcttggaa gagaccattg cccaacagag
      961 cgagcaaagt ctgaccttga aagagttcta cctgctctac tatctggact tggctgaaga
     1021 aaaatctcta cgccagattg acctgccaga taagcttcat ctgagcccga gcgcagtttc
     1081 taggatggtg gctcgcttgg aagctaaaaa ttgcggtcta ctcagtcgca tgtgttgcca
     1141 tcaagataga cgctctagtt ttatctgcct gacaaatgat gggcaaaaga cactggcctc
     1201 gctacaaaag actgtcgaag aaagcttgga agcaggtttg gatttcttga tttaagatga
     1261 tttttaaaaa agttgcgtgc gcaatcattt ttcttgacat tctcttttat aaggagtaaa
     1321 ataaagttag cattaaacaa aggagtttta aatatgattg aaattaccta tatagatgcc
     1381 agcaagaacg aaagaactgt gactttcgag tcttatgaag actttgaacg ttcgcaacaa
     1441 gcttgcctta tcggcgtcgc agactactac cctgtccaaa aattaaccta caagggtcat
     1501 aatttggact accatgggac ttatggagat atcttcttct atctcatgaa acaagattta
     1561 agccaatata actaaaaaaa ggagaaatac aatggcaaaa gcaattacag atgcaacatt
     1621 cgaacaagaa acaaaagacg gtttggtctt agtagacttc tgggcaactt ggtgtggtcc
     1681 atgtcgtatg caaggtccaa tcttggacaa attgtctgaa gaactttcag aagatgtctt
     1741 gaaaatcgtt aaaatggacg ttgatgaaaa tccaaacaca gctcgtgctt ttggaatcat
     1801 gtctattcca actcttctct tcaaaaaaga cggccaagtt gtcaaacaag ttgcaggtgt
     1861 tcacacagca gaacaaatca aggccatcat tgctgaattg agctaatcaa acgagagacc
     1921 aagtactttc tttggtctct tttttcttgc cctttgccat ttttcaaaaa atatgctaga
     1981 ctatagtaga ttgaaactag aatagtacat ctctacttct aaaacattgt tagaaatcga
     2041 tttgactgtc ctgatcgatt tgtcctgttc ttatttcact ttactatata aatagaatat
     2101 tacgatattt gggatatgcc atcgctaaaa aaaacctcga tttggtattt tttagctccc
     2161 taatgggagc tttttacgtg ctctgaacat ttcccatttt ggaaggagta ctatgaaacg
     2221 tcaatcagcc ttggtcgtct tagtggtggt caagattcaa aaacctgcct tttctggacc
     2281 atgcaacact atgaaacagt cgaagctgtc acctttgcct acggccaacg tcatcacctc
     2341 gaaattcaaa ttactagaga aatcgctaag gaacagggca ttcgtcacca tatcctcgat
     2401 atgtctctgc tgggacaaat cactgctcag ccagactttg cgacgattca tatttcctac
     2461 attcctgaca agctctgtgt cgagtcaaaa tccctcaaac tatatctatt tagctaccga
     2521 aaccacggag atttccacga aaactgtatc aacaccatcg ggaaagactt ggtcaacttg
     2581 ctagaccctc gctatttaga agtctgggga aaattcactc cgcgcggtgg catttcaatc
     2641 gacccctact ataactacgg taagcaagga actaagtatg agggcttggc agaacaacgc
     2701 ctcttccaac acgaccttta tccagagaaa attgacaacc gctaaagtca tactcaatga
     2761 aaatcaaaga gcaaactagg aagttagccg caggttactc aaagcagtgt ttgaggttgt
     2821 agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgcagata gaactgacga
     2881 agtcagctta aaacactgtt ttgaggttgc agatagaact gacgaagtca gcttaaaaca
     2941 ctgttttgag gttgtggata gaactgacga agtcagctta aaacactgtt ttgaggttgc
     3001 agatagaact gacgaagtca gcttaaaaca ctgttttgag gttgcagata gaactgacga
     3061 agtcagctta aaacactgtt ttgaggttgc agatagaact gacgaagtca gtaaccatac
     3121 ctacgacaag gtgaagctga cgtggtttga agagattttc gaagagtatc atacgaaaaa
     3181 gccctgttcc tcaagatgag gagcaaggct ttttgagttt caattattct tctgttccaa
     3241 ggaaattttt tgcaacaagg gctgcaagaa ctccaccagc gattggtgca aggatgaaaa
     3301 tccaaacttg ttgaagggct gcgccgccta ccaagacagc tggtgccaag ctacgagctg
     3361 ggtttactga aagtccagta atcttcaatc cgacaagaat catcgccatc aatgacaaac
     3421 cgattaccaa accagcaatc gggccattgc ccttgctttc tgaagtcaca gtcatgataa
     3481 ccaatacaaa caagaaagtt gcgatgactt caaacaagaa accaccaaag acagtgacac
     3541 cgtttgccaa ggcattttca ccaagactag cagttgacat acctgagtta gccaagagga
     3601 agaagacagc gccagaagcg atgaaagctc caacaacctg accaaggatg tagtttacaa
     3661 gttctgaaga tgacaaacgt ttgtttacaa acatagcaat cgaaacagcc gggttcaagt
     3721 gagcacctga aacagttccg attgagtagg ctgccaccac gattgccaaa ccaaaggcaa
     3781 aggcgattcc aaggtgacca aggccatcaa aaccatttcc aaaaacaaca gctcctgtcc
     3841 caacgaacac aagcatgaac gtaccgatta actcagcaac aaattttttc attttctttc
     3901 tccttttttc aaaaactaga tactagtcta tcaaaagtag gaaagggttt caagaaaatt
     3961 gattggaaat tttttgaaaa tcatagaact attagctaat ctctagtatt gaaaagactg
     4021 gatagcttct ttcaactcat cttgtaaact atttctctgg tcaagttgga catagacttc
     4081 caccagacag gatctaaagt tggaaaattt ggaaaaatcc tccctttctt ctatcggaaa
     4141 atcaacagtt tttatccaag aagctacttg ttcttgctcc aacttccctt gtaaaatagg
     4201 ttcatagatc actcttgcta aacgccaatc ctcatcatct gtaaagcgaa tcgacattct
     4261 tttaaatagt tggccaagta tgtcaaatac ttcatgaatt ctgtttatag gaaagtctgg
     4321 atgacagacc acctctgtca gtaaatcggc tccatgtgca aaagcatgaa cccaaccata
     4381 ctgacttgag aaacctgttg tatccttttc ttttgaaaga tagtgcaagc cttgatttga
     4441 aaagacatta cgaatttcta attgtaatcc ctgataaaaa accgattgct ggttggcatc
     4501 tgcatacaag agatttgcat aaacaagtgc cctaaaagaa cgttcaagtg ttggcagacc
     4561 taccttatca atctctttat ctagtcctac atcagatgaa accccttcag caatgaaatg
     4621 aaattgctcc tgtgtaaata gctcttcctg aatccctata gcaaagcttg taaaaacaag
     4681 gtcatcgcga atttctggag aaggatctcc caaatgatca agcaaccact ggatttcctc
     4741 ctggttataa tttggttttt cttctgctat ttttcttagt aaatcttgat acatggtcaa
     4801 tacctctaca tttctagcaa ctgttcaaaa aggcagtctt aaatgactca atattgaatt
     4861 ctcaattaaa tacaatctga tataaaatga cgtaaataac tatcaatacc agttctacag
     4921 taagttcaaa tttaacatca cgaccttcaa cgacattttt gaaaatagct acaactaaga
     4981 caaatagaat gacgcttaac aagcccataa acatcattct aaaaaatttt tctattcccc
     5041 ttactccccc aactcagcac tgtaggcgat aatctggtca actgtgtcag acaagaattg
     5101 gatggtatca cggagtggtt tgtctgttga aatatcagca ccgataatca tggctgactc
     5161 aagtggtgtc ttgctaccac ctgatttgag gagattgagc cagtcttcag ctccagtttc
     5221 tgagttttta agatgaaggt aaccagctgt cgagatgaca agaccagcag agtaagtgta
     5281 actatacaag cccatatagt agtgagcttg gcgcatccaa gtcaaagctg catcgtcgtc
     5341 gatttcaatg gcatcgcccc aaaaatctgt caaaacttcc ttcataatac tgttgagttt
     5401 gcttgctccg aaggtttctc cttcttcaat caatgtatac accttacgct ggaaggcggc
     5461 ttccaagagg tgggtgataa agttatggaa gtaggtgtct gtcaagcgat gagccagagc
     5521 aaagcgtttt tgacgtgggt cattagattg gttctctaag taatcactga gtagcaattc
     5581 attgaaggtt gacggtgctt caacatagta ggtcgacata tgggcattga agtaactttg
     5641 atgattgtct gaaaagatga attgaccaga atgcccgatt tcatgaatca aggtatagac
     5701 atcgctcaaa cggcctgtcc agctcatgag gacataaggg tgtacgcgat atgggtccgc
     5761 cgcataacca ccggaatcct tgccactgtt agcagcaaag tccacccagc gctcttcttg
     5821 gtaacgagca acttcctgac aatattcttg ccccaaaggt tctaccgact tcatgaccaa
     5881 atcataggca tcgtcaatag tcacttcagg attcagggcg ctgtccaagt ccaatttcca
     5941 gtctgcaaag gtcatctttt caagaccatt taccttggca acatgcttga ggtatctctg
     6001 agcgactggt gcaaagtcct tcatgatgag gtcaatctgg cggtcaaaca tgacacggtc
     6061 cacttcttgt tcagctagaa gatagtcaaa gacagagtcg tatcccttca tatcagccaa
     6121 gagtttttca gacttgacct gagccagata ggctgctgca gccgtatttt ggtgcttacg
     6181 aagtccctct gagaaggaac ggaaggattt ctcacgaacc tcagcatcct cgtgattttg
     6241 gtagaaattc tcataggtga caaagctgtt tttataggtc ttgccatggg cttcaaagtc
     6301 agccatttca aaatccccag ctcgcatctt agtataaatg tcctgcggac tatagaaaac
     6361 ttcaccgaga ttggtcaagg ccttctccac atcagcccct agatagtggg cttttttgat
     6421 tttggcctga cgaatagctg ccgtcaagtg aggcaattca cccaaacggt ccaagacttc
     6481 ctcgtctgct accaccaagg catcgtcaaa gaaggtcaag gctacgctgg catctgtttc
     6541 aaattccatc ccagcttggg caatattggc aaattcgtca ttgctatagt ccgtcgtctg
     6601 aggcataaaa gcatagttgc caatatggct catctgaatg tagatctgtt ccaattccgc
     6661 aaaggccttc tcgaaatcct caaaagtatg aagattaccc ttgtagtcac ggctaaattg
     6721 gttgatgtct tcacgagctt tctcgattgc acgcaagaaa tcctcacggt cttggtatag
     6781 ggctgttaag tcccagagtt ccttctctgg aaattctgaa cggtgttttt gttccatttt
     6841 cttcctctta tttctctaat tctactaaaa cactaagggc tgataaggcg taaagcggtg
     6901 ctgtttctgc tcgcaaaata cgaggaccta ggcctgccaa aacggctcct ttagcttcaa
     6961 aactttcgat ttctgcaggt gacagaccac cttctggacc aaagataaag agcagtttgg
     7021 atcctgtttc aagaccagtg actgcttgca gaagcgcagc ggcttctcct tctttagctg
     7081 attcttcata ggctactatg atagagtcaa actggtccag ctgagctaga aaatctgctt
     7141 ttttctcgaa aagtttaata cttggtacaa tattacgctt gctttgctcg gctgctccaa
     7201 gggcaatttt ttctagtttt tcaacttttt tacccaattt cttgccatcc cacttggcaa
     7261 ctgaccagtc tgcaggaaag gcccagattt ggctagcacc cagttcggtt actttttgag
     7321 cgatgaactc cagcttgtct cccttgggaa atccagatgc gatggtcact tggactggta
     7381 gttccacatt gtcatttaat tcttggacca actcaaactg acgattttcc atatccagca
     7441 cgcgcgccaa gcgcttgatg ccatcatcaa agactaaggt aacctcatcc tcttctttca
     7501 agcgcataac ctgaaacata tgcttactgg tttccttgtc ctcgatagtg acaggagaga
     7561 tagcactgcc ttttacaaaa tactgctgca tgctagcctc caatcacacc agagatatcc
     7621 ttggttttct taaagacaca ggtattccat tccccttgaa ccatgtgagt ttcgaggaaa
     7681 aatccagctg attcagccga ctcgcgcacc atgtcccact tatccttgat aatgccactc
     7741 atgatcaggt agccttcgtc cttaaccaaa cgataggcat cgtctattag atgaatgagg
     7801 atatccgcca agatattagc cacaatcaca tctgcctcaa tttccacacc cttaagcaaa
     7861 tctccagccg ctacatggat attttccatg ccagggttga gctcaatatt ttcctgagcc
     7921 acacgaaccg ccacatcatc caggtcatag gcgaaaattt ctttagcccc cagaagcgag
     7981 ctggcaatag agagaacccc tgaaccagtc cccacatcta gcaccgtttc gccaccacga
     8041 agaacctgtt ccaaggcaaa aaggctcatc ttggtagttg ggtgggttcc agtaccaaaa
     8101 gccatgccag gatccagctt gataatcatt tcccccgcag tcgcctcata gtctgtccaa
     8161 gagggaacga tggtcaaatc atgagtgata cgagctggct catagtattt cttccagttg
     8221 tctgcccagt cttcctcagc caaggcagtc gtacctattt ttaactctcc caaatccata
     8281 aaatccgtca attctgctaa gcgagcctgc aagtccgcct caaccactgt cacatccacc
     8341 gtgtcagggt agtaggctgt cactacgatt tcttcttgct gctccacctc tgggaaaatc
     8401 tcgccaaagc ggtccatatt tcccacatag tccatgctgt cttcaatcgc aacaccttgc
     8461 gctcctagct caatcaagag attggaaacc aactcctctc cctcacgctt cactgtaact
     8521 tttaactctt gccatgtttc cattattaag ataccaagcc cgtaaaacac aaaaccaaaa
     8581 taggaaattc tctgaagacg tttgtgtcta agagaagttt atctttttgg caccgtgttt
     8641 agggcgggtt cagtttagaa atttaaccga accatccttt ctaatcactt acttttaaat
     8701 aatcttttaa tctctcttgc aactgaggca caacttgact ggaactaaga aattcctcaa
     8761 cattcatcag ctgatagccc tgtccttcat ctccaaatac gatactgtca aactggttct
     8821 gtgtcaactg accaactaga aaaacggact gtttgtctgc aaaaagcata cttgggtaaa
     8881 tcttactcca aagcagacaa tcttcagtca gatgaatgcc cagttcttca taaacttcac
     8941 gcgctacaca ctcaaaagga ctttcatcac cttcacgtcc accacctggc agttcccaca
     9001 tattggccca gggaatgctt gccttatcat cgcgtaagat agtcaaaagc ttatccccac
     9061 aaaacaaagc aatcttgcaa cctgtgaaat cagaaatctc tatttccata ttagactcct
     9121 tcggctaatt ccttttccag ctctgctaac aagttttcat aatagcgttt ctcgtccctc
     9181 aacattcgac tactatccat tttctgtcta gcaatcttga gagccttacg agttcgatct
     9241 acatctttct tcacctttaa ttgataccag gcttgtatca cttgaagatt ggacagtttg
     9301 agagacagaa acgatttgac ctgtcgaata ctggcatatt gctccgcttg ctcaaaatct
     9361 ccttccaaca aggcgatatg aagcagggat agttgggcaa ctgtctgcat catcggagta
     9421 gttgtcctct caagtaatgc ttgaaactgc tgtttagcta cttcttcctt cccttccaaa
     9481 atggaaactt caccttgcat acctaataca ccatccgcaa aactccctcg tgcatcctca
     9541 ggaactgctt gaacaaagtc tttcaaatca tattcttgag gagccagcaa agtttgagca
     9601 gaatgtctca ataccaggta ggcgtatttg gtattttcag ggtgttgtag taattcccaa
     9661 atttttgctc catcggtgat gtcgactggc aaaatgttat ttaggaagaa agataaatta
     9721 agaaaaatcc aagtccctgc aaaataccag cttcttgtca aaaatccaaa cactatcgcc
     9781 aataatatca agccgagatg aaccatcaag cctcctgaaa gcatcaggat gattctttga
     9841 tcgctttcat cctcttttaa accaatgtat tgagcaccaa catttttcag aatggctgtt
     9901 ctactaagat gaaacctgcc tgactttttg gtcaaaataa aatgtcctaa tccaaaagcc
     9961 accagccgat agcctgtcaa gtagccacaa aaagcatgac ccagctcatg aagaataaag
    10021 attaaataca tgcttagaag agcgaaggca taaccaaaag taaaggctaa aactgcggaa
    10081 taccccaact ctgcaaatgc gattgttcca caagcaaaag ctagcataat aaagacaaca
    10141 gctagcacat aaaccaaata agtcccaatt ttcttcataa cacctccaac caactcctag
    10201 tatcttggat aaggataaaa ttctccctct tccaagccaa cttttccttc ttcaaagact
    10261 tcttgattcc attccataac aaattcctct gcttctggat cttccaaaaa gtccatgagg
    10321 gtatctagcc caacctccgc agtatctttg aggaaaaggg caaaataagc taaaaactcg
    10381 cgagaaaatc cttttttagg tagataaggg ataaccgtta aatagtcttc tgcattgacg
    10441 gttgacttgg caggattgta gaaaaggacc gcttcctcaa aaagaatgtc atctgatgaa
    10501 acctctccgt cttcatccac catctccaca cctgcagcat tttgcgcttc caatagaaaa
    10561 ctcacttcta ccgcgtggtt acgcttgtcc caactaatct caaagtcaaa gggaaagttc
    10621 ttgtccaact cttcctctaa aatatctaaa aatccgtatg ttgccatttt gtcctctttc
    10681 tatgcgactc tttaatcgcc ccgattgctc ggaaatatgc taaaatagat actaccatct
    10741 taccacaaaa ttattttatg tcctaattat accatattac ctcatttaaa cccttggtat
    10801 cagtgatttt cttaaaagtc tgatttcttc atttctcata aaaatcaata taaaaagccc
    10861 tcgaaagggc taataaatct ataaaatcaa taggcgagta actagcacaa gtggacgtgc
    10921 ttttttattg actattacca cgataccacg cttaatctta ggcttgaact ttcttatctg
    10981 caatagcgtc tgtcaaagtc tgagaaaagt taagtcccat ttctcgtccc aacttatctg
    11041 cccattttgg tatggtcaaa gtctttttaa tgggttcctg acttcctagg tattctgata
    11101 catcaacaga taccatagaa ataaaagatt tatcaaggtc ataggttgac acgaaatctt
    11161 catcatcttt aaaaggatca ttatcaatta aagacaagct attgatatct gatggctgag
    11221 gtaactctcc atcactctct atcaaatctg caacagttat ccctagccac tccgacccca
    11281 tagccaaagc ctcagaaatc ccctctcctt gtgtagctga gtattcaaaa tctgggaaat
    11341 ggacaaaata agtcgcttct gttccgtctg tgtcgtcata ataaaataaa gctggatacg
    11401 taactaacat ttcactacct ccatatcaaa aagcagggac tgaattttac aacccagctt
    11461 gctttcctat ccctctttca gtgtacttat tcagctcacc atgaaggatt gtgataggtc
    11521 tttccccttg cttctcgatt ttaatatggg agcctttacc gcctctagtc tttatccaac
    11581 catgggccgt aaggagttta accatctctt tttgtgtcat aggtatagcg cttttacctc
    11641 ctgacaacac cattataaca cgtgttacac gtattgtaaa ggagtgatac ttattattct
    11701 attatacata aaagccccta gatgtggttc taagggaaac caatttattc atacctattt
    11761 ttctaatgag tagtaaaaac tgcttcttta tcgagcaatt catcatctgt atagtcaatt
    11821 gtaaaagtat ctcgatctaa gacagattga ggcggagttg aatgaatcat aggaccactg
    11881 cgtactctat attttttatc tccaattttt acaaactgat actcttcgaa aatcaaattc
    11941 aaaccacgtc aacgtcgcct taccgtactc aagtacagcc tgcggctagt ttcctagttt
    12001 gctctttgat tttcattgag tatgattaac tctcaagtct tcgaaatcag gattttcaac
    12061 agttattaca aggaggcgat ttactacttc aaaaacatca attattctat ttttcatatt
    12121 ttttcaaccc attattagaa tgaacttctt ggtaagcaaa atcaagttta gatttaatgt
    12181 tttcgtacaa atctaaaatc tcttttggag tatcttccgg aagaaaagtt ttcttttccc
    12241 tgaaataact tgatcactaa gaatccaatg acgaatttgt tttgtaaaaa tcaaaatttc
    12301 ctgacttggt ccatcatttc cattgcttat cacctctctt ttcattatag ttcatacaat
    12361 gacattcagc aatattattt ctcaagtcag cacttccact tctttaggct caactatcct
    12421 attttgagct ttaaggaaaa tcaaatctct catgctgata cctctcctca ttaaattaaa
    12481 tagtaaaaaa gattctatct cactccctga ttattacaaa accattgaaa tatcacaact
    12541 aataggctag aatggacata gtaagatata gtagatgagt cattctactc aaatccacgc
    12601 tagaaaggac tgctatgcca gacaatctcg cgcttcgcat gcgccctaaa accatcgacc
    12661 aggtcatcgg tcaggagcat ctggtcggac ctggaaaaat catccgccgc atggtggaag
    12721 ccaaccgcct gtcctccatg attctatatg gccctccggg aatcggcaaa accagtattg
    12781 cctctgccat cgctggaacg accaagtatg cctttcgaac tttcaatgcg acagttgata
    12841 gtaaaaagag actgcaagaa atctcggaag aagctaaatt ttctggtggt ctcgtcctat
    12901 tgctagacga aattcatcga ctagataaga ccaagcaaga cttcctcttg cctctcttgg
    12961 aaagtggact ggtcatcatg attggagcaa cgactgaaaa tcctttcttt tccgtcactc
    13021 ctgccattcg tagccgtgtt caaatttttg agttggaacc tctgtctaac caagacgtca
    13081 aagaggccct gcagatagct ctaagtaacc ctgaacgtgg ttttgatttt ccaatagaac
    13141 tagatgagga tgcgctggat ttcatcgcaa cctctacaaa cggagacctt cgctctgcct
    13201 ttaactcact ggacttggct gttctctcta cccctgagaa tgacgagggc attcgccaca
    13261 tcaccttaga catcatggaa aatagtcttc agagaagcta catcactatg gacaaggatg
    13321 gagacggtca ctatgatgtt ctatctgccc tgcaaaagtc tatccgtggc tcagatgtgg
    13381 atgccagtct ccactatgct gcccgcttga ttgaggctgg ggatctgcct agtctcgctc
    13441 gtcgcttgac tgttatcgcc tatgaagata tcggtttagc caatcctgag gcccagattc
    13501 ataccgtgac tgctctggat gctgcacaaa ggattggttt cccagaagcc cgcattctca
    13561 tcgccaatgt cgtgattgat ttggcccttt ctccaaaatc caactcagcc tatgtagcta
    13621 tggataaggc acttgctgac ctcaaagcat cagggcactt gcctattccg cgacacctgc
    13681 gtgatgggca ctacagtgga agcaaggaac tggggaatgc ccaagactat ctctatccac
    13741 acaactatcc tggaaattgg gtcaagcaag actatctgcc agaaaaaatt cgtaatcatc
    13801 actatttcca agcagaagat actggtaaat atgaacgggc tttggctcaa agaaaggaag
    13861 ctatcgacca tttgcgaaaa atctgaaatc cttttcaaaa aactgcactt tcctcttgat
    13921 tttttttgaa aaagtggtat catataaata tagaaacgct gtggtgtacg acttcacact
    13981 taagtgttga ccgactattt tttgtattat tagggaaaca aaagtcttct aacagcatgt
    14041 aggccgtctc acacggaaac agcttcagtt atagcgagtt gcccacctgc ttaattgcgc
    14101 gggttcaata caaaccgtga agtttcggca ccaatacagc tttttatttg ccttcttagc
    14161 tcagttggta gagcagtaga ctcttaatct atgggtcgca ggttcgagcc ctgcaggggg
    14221 catctaaatc aacaggaaaa agccttataa tatagggctt tttttgctta aataaaattt
    14281 ttttcaaatt ctttagaaaa ctagtttaaa tcataatcca acaccaattt tacaaatacc
    14341 ataaactaag cttatatacc caaaacacaa aaagtcctct ttccactatt ttagaaagag
    14401 aactttttct ttaggagaaa taacttaatt tttaatcaat tcgtgatagc tcagctggca
    14461 ccgttaagta ataagttgca gtcaagcttg cttcaccttg attgacaaaa atctcgatgg
    14521 aatttttatc tagaacaact tccaattctt tagcttcaat atctacatac cgtcgactag
    14581 tgtcctgttc ttcttcacct agaatttttt gaataagatg gctacgatca atgtaaactt
    14641 gctgcgcatt actgtcataa ccaaattcaa gataatctgt atcatttcct aagtggtaat
    14701 gacaatcttt atctatttgg atttgatatt ggcctttttt aacagggaat tgtcttagtt
    14761 tgccatcttc caatcttaga attctaggta aagtcatggc acatgcccac ttgtgttctt
    14821 ggtcatgggt tggaagggta cgcccccatg tctgcatcca agcaatcagg atacgacgat
    14881 tttgatcgtc caacaatgtt tgaggcgcat agaagtcttg gccatgatca atttcttgaa
    14941 ctgattctgg gataaaacat ttttctcccc actctacctt acccgtgaac aaaagcgatg
    15001 agttgatgtt atgatatgag tctccctcac gctgataacg catgggtgac ataataaggc
    15061 aatctttccc atctaactcg aagtaatctg ggcattccca cataaaccct tggtgttcta
    15121 ccccttttaa aaagatggat tcgaactgcc attctactag gttatcggac cctagtagaa
    15181 cgatacagcc cacattatcc ttgtgtttgg cagctactat ggagtaatag cgtccatctt
    15241 tttcaaagag ttttggatca cggaaatcag cagcaatcaa ctcatctggc aagtctgctc
    15301 ctgttgcaac tggattttgt tcaatctttt caaagtgaat cccgtcgtct gaaaatgcca
    15361 tattttgcac ttggcggaca ccgttttttt cttcgatatg cccagtgtac atgagccaga
    15421 ggcgatcatc cttgacaatg gcagagcctg agaaacaacc atttcggtca taatcttgat
    15481 caggagcaag tgccactggc aagtgctccc aagtcaccaa gtccttactt ttagcatgtc
    15541 cccagtgcat aggtccccaa acactatcat atggatagaa ttgataaaag agatggtatt
    15601 ctccacgaaa ataaacaaat ccatttggat cattgatcca accaatctca gctgaaaaat
    15661 gttcttcagg cttatattga gtattaacga gatgtttatt ttctgctata aaacgattgg
    15721 ctttttctac tgtgtatttc ttatctccca taatcaacct ctagtttttg tttgcttggt
    15781 attggtcgta gtatttttgt ttaatagcga ggtaatcaga aagtccgtat ttttcaagtt
    15841 ctttcttgta atcatcccac tcagtatcaa tattgccatt tacaatccat tcagcacgtt
    15901 tacggtagat atagtcattc atatctgctt cgatatgggc aatcttgtcc aaatcttcct
    15961 gtgtcataaa gactcttgga tagttattgt cattgctcat gtaaggaaca taatattctt
    16021 tgataagatc caaacgccat ttggcatcat caggcatggt tgttacttta ccatagtatg
    16081 aatctaggat agctagtggt cctcctactt cagtcttttg acgaagttct gctggtgcag
    16141 ttccgtttag tggtaagtgt tttagactat ttgacgcttg atccaattca aagatgtttt
    16201 gttgtttgtc atctccgtaa gttccccagt tattttgcac agattggagt ggagcgtatt
    16261 gtgcatcaat ccatttagct gtcaattcta ggtttttgtt tacactggta ataaccatct
    16321 tgtcacgtgc aaatcccata ccgtttgtac gagctacgtg tttttgacca cttggtccag
    16381 caagtactgg taaaacatca taactttcgt tacttccagt aacattattc ttatcccatg
    16441 taaagtaaac accaaatttc tgatcatgac ctttagcaat gtaactattc caatcatgtt
    16501 cgaaagcttc tttatcaatc aagccttttt cttgcaattg acggataaat ttgacacctt
    16561 ctttatagtt atcgttatct gctgtgaaat caactttgcc atcatttcct actactaaat
    16621 gatcatcgtt atcccctata ccaaatgcag caaataggaa tttaaaatct tcgtttccgt
    16681 taccactaat aaatgaaaat ggaatttcat cagcctctcc atttccattt ggatccccgt
    16741 ttttgaaagc ttctaggact ttaatcaaat catcagtagt ttttggcatt tcaagaccaa
    16801 gtttcttaag ccaatctttg ttaatccaag ccatatcgtt gacgctgtga atagactctt
    16861 taccatctcc aagctcttca atccatggga atgagtaaat gtgcccatca ggtgctgtca
    16921 tcaaggcctt gtactctggt ttctcatcca aaattttctt aagatttggc atgtatttat
    16981 caatcaaatc ttcaactgga ataataacac cttttttagc ccagttcatc aagtccacat
    17041 ctgaagctcc gtcgttgtgg atagcatctg gtaaatcacc actagaaata tccaagttac
    17101 gtttttctgc aaagtcggat tggtagttgg tccagtcaat atgaacgcca gtttccttct
    17161 ccaaacgttg caaaattaac ttttcatttg ggtctttagg agataacggt gaactggctg
    17221 tcataaactt caatgttttc ttttcttgaa gcgggaatgt tacaccttcc aacttataat
    17281 ctggacttga agctgtattt tttgagccac aggctgcaag tactgctaaa ctagctgtca
    17341 acaaaactgc tgattttgag aatgttttga atttcatgag aaaactcctt ttattttttc
    17401 tttttttatc ctttaagtga accagccata attcctttat caaagtattt ttggaagaat
    17461 ggatacataa caatcaatgg caagctggaa atgacaatag ttgcgtattt aatcaattca
    17521 gctaaacgtt tcatttcatt catagccgct tgtgctccaa tcatgtcttg acctggttgg
    17581 ctctgaatga gaattttacg aagtacaagt tgcaatggtt ccaagtttgg atccttgata
    17641 taaatcattg catcaaagta tgagttccac tgtcctacaa aagcataaag gaagagaaca
    17701 aacataattg gttttgcaag aggaagcatg attttgaaga aaatctgtaa atcatttgca
    17761 ccatcaatga cagcagcttc aactaattct tcaggcaatc cttggaaata ggccctagca
    17821 agaataatat tccaaacatt gacagcacct ggaacaatga tagcccatgg agtattgagc
    17881 attcccaatt cttttacgag caagtaagtt gggactaaac caccaccaaa gaacatagtt
    17941 acaatcaaga agtagttaat ccaacgacgt ccaaccaagt ctttcttaga aagaggataa
    18001 gctgtaaaca cagatagcaa gactgttaaa gctgcaaatc caaaagagta tagtagagaa
    18061 ttgataaaac ctcttagaat agattggtca ctgaatacac gctggtaacc ttctacagtc
    18121 caatcggctg gattaaagct aatccctcta ctaaccagaa ccttaggatc cataaaggat
    18181 gctacgacga tataaagtaa aggaagcaaa gtcatcaaaa cgataaagac aataatgatt
    18241 ttatttaaga gtaagatacg tctatcaaat tttgtatcca taatcgaatt tttcatactt
    18301 tcctccttaa attccttcac cattattcat gcgtttaacg atttggttaa ctgcaacaag
    18361 caatactacg ttaatcactg cattaaacaa accaaccgct gttgagtaag aatagtctcc
    18421 tgatacaaga ccaactttat agacatatgt cgagataatt tcagaagttg gcaaatttaa
    18481 cgatgtctgc atcaagaatg ctttttcata tccgacattc ataattccac ctgcagctaa
    18541 aacaaattgg ataaccataa taggcttaag agctggaata tcaatgtgcc agattcgttg
    18601 gaagatattg gctccatcca gtcgagctgc ttctactaag gctggatcta catttaccaa
    18661 tgttgccgtg tagagcgttg aagcccagcc cattccttgc cagataccac taaagatgta
    18721 taagggtcta aagaagtctg gatttgtcaa gaagtcagcc ttcattccaa acatagaaag
    18781 aaaattgttg attggtcctc ccactgaaaa gaagaggaaa atcataccga caataacaac
    18841 gactgagata aagtttggtg cgtataaaat gagctgaatt cgttttttga ctttttcact
    18901 caagagttga ttgagcataa tcgcaagaat gattggtggt aaaaagccaa gcaataaacc
    18961 atagatactt aattttaatg tgttggctaa caagatacca aagttgggag aggatatgaa
    19021 ttttgtaaac tcagaaaaac caatccaatc actccctaaa attcctttta aaggattgta
    19081 atctttaaat gcgattaaaa ccccatacat agggatgtat ttaaagataa aggttaaaac
    19141 taaaccgggg acagtcatca ataacaagag tttttgtttc ttgattcttt cccaaagaga
    19201 aacttgcttc gctttgctat tcatagcaac ctcctttttt tgaaacgttt cattttttct
    19261 ataaaagata gcgcttggcg ctgtcttttt atttcttttt ttgaa
//
LOCUS       DAVHEW010000032        19014 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVHEW010000032 DAVHEW010000000
VERSION     DAVHEW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.032"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            282..665
                     /locus_tag="VVL53_001027"
     CDS             282..665
                     /locus_tag="VVL53_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU4183215.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            717..1406
                     /locus_tag="VVL53_001028"
     CDS             717..1406
                     /locus_tag="VVL53_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183216.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1448..1696
                     /locus_tag="VVL53_001029"
     CDS             1448..1696
                     /locus_tag="VVL53_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183217.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            1726..2337
                     /locus_tag="VVL53_001030"
     CDS             1726..2337
                     /locus_tag="VVL53_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183218.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2498..3292
                     /locus_tag="VVL53_001031"
     CDS             2498..3292
                     /locus_tag="VVL53_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU4183219.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3289..3924
                     /gene="trmB"
                     /locus_tag="VVL53_001032"
     CDS             3289..3924
                     /gene="trmB"
                     /locus_tag="VVL53_001032"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU4183220.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4050..4529
                     /gene="rimP"
                     /locus_tag="VVL53_001033"
     CDS             4050..4529
                     /gene="rimP"
                     /locus_tag="VVL53_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU4183221.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4573..5709
                     /gene="nusA"
                     /locus_tag="VVL53_001034"
     CDS             4573..5709
                     /gene="nusA"
                     /locus_tag="VVL53_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU4183222.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5731..6024
                     /locus_tag="VVL53_001035"
     CDS             5731..6024
                     /locus_tag="VVL53_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU4183223.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6017..6316
                     /locus_tag="VVL53_001036"
     CDS             6017..6316
                     /locus_tag="VVL53_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU4183224.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6333..9209
                     /gene="infB"
                     /locus_tag="VVL53_001037"
     CDS             6333..9209
                     /gene="infB"
                     /locus_tag="VVL53_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU4183225.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAAPKASAEKKAE
                     KSEPVKPAVAKEEAKPAEPVTPKTEKVAAKPQSRNFKAEREARAKEQAERRKQNKGNN
                     RDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQQGQQKRRNERRQQEDKRSNQA
                     APRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKEALAQANKRKEPEEIFEEAAKL
                     AEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRDDYDHEEDGPRKQQKNRSSQNQ
                     VRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFHELPTEFEYTDGMTVAEIAKRI
                     KREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYGIEAKQKVEVDNADIERFFVED
                     GYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIVENG
                     KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIV
                     AINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVEISAKFNQNIEELLETVLLVAE
                     IQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTN
                     DLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEKSARAAGEERAKRALMKQRQAT
                     QRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSASLQKIDVEGVKVTIVHSAVGA
                     INESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVEIRLHSIIYKVIEEMEEAMKGM
                     LDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKVARDSKVRVIRDGVVIYDGELA
                     SLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAYVMEEIKR"
     gene            9460..9810
                     /gene="rbfA"
                     /locus_tag="VVL53_001038"
     CDS             9460..9810
                     /gene="rbfA"
                     /locus_tag="VVL53_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU4183226.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTIIDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10089..10868
                     /locus_tag="VVL53_001039"
     CDS             10089..10868
                     /locus_tag="VVL53_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183227.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRVGSITIAK
                     QVMKIVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            10870..11052
                     /locus_tag="VVL53_001040"
     CDS             10870..11052
                     /locus_tag="VVL53_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183228.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11407..11481)
                     /locus_tag="VVL53_001041"
                     /pseudo
     CDS             complement(11407..11481)
                     /locus_tag="VVL53_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11512..11745
                     /locus_tag="VVL53_001042"
     CDS             11512..11745
                     /locus_tag="VVL53_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU4183229.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11745..13079
                     /locus_tag="VVL53_001043"
     CDS             11745..13079
                     /locus_tag="VVL53_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU4183230.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13089..13343
                     /locus_tag="VVL53_001044"
     CDS             13089..13343
                     /locus_tag="VVL53_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU4183231.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            13439..13834
                     /locus_tag="VVL53_001045"
     CDS             13439..13834
                     /locus_tag="VVL53_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU4183232.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            14247..14696
                     /locus_tag="VVL53_001046"
     CDS             14247..14696
                     /locus_tag="VVL53_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU4183233.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            14683..15243
                     /locus_tag="VVL53_001047"
     CDS             14683..15243
                     /locus_tag="VVL53_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183234.1"
                     /translation="MARTELPDKIETERLVLRVRTVVDAEDIHTYASLPEVAYPAGFP
                     PVKILEDEIYYLEHILPERTQKENLPAGYGIVVKGTDKVIGSVDFNHLHADDVLELGY
                     TLHPDYWGRGYVPEAACSLIDLAFKELGLHKIELTCFGYNLQSQRVSEKLGFTLEARI
                     RDRKDAQGNCCDDLRYGLLRSEWEVI"
     gene            15243..15821
                     /locus_tag="VVL53_001048"
     CDS             15243..15821
                     /locus_tag="VVL53_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4183235.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQDLLDNFHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQHQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            15814..18465
                     /locus_tag="VVL53_001049"
     CDS             15814..18465
                     /locus_tag="VVL53_001049"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU4183236.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            18546..18710
                     /locus_tag="VVL53_001050"
     CDS             18546..18710
                     /locus_tag="VVL53_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU4183237.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            18761..>19014
                     /locus_tag="VVL53_001051"
     CDS             18761..>19014
                     /locus_tag="VVL53_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4183238.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEK"
BASE COUNT         5730 a         3565 c         4346 g         5373 t
ORIGIN      
        1 aagtaaatga tgatttggtt agtcctagaa ccaatcaact tatgtttatc ggcttggcag
       61 attttatgtt tgtcatttgt ttgtatagag gtatttcaga aacagaattc tatcaacaac
      121 taatcgcttg tataggtgta ttctctgctt gtttatcacg cttctgctca tgtggggctt
      181 gaagtattat aaaaagtagc tttctatcct tttctgacag gatatttgag tagaaggctc
      241 tattaaattg ttagtgaaat aaatgaatgg aggtatttaa tatgaaatac agattatttt
      301 ttgttatttt cttgagtagt gtgttggata ttcttttagg gacattttta caaatctcta
      361 tcgtatctat tgggtggctt gttctttaca gtggtttgtt tgaagcagga gttttccttc
      421 ttgctaataa aggggtggcg gtaaaaatca aggaagtaga tattcgaaat cgctttaaat
      481 ttatttttgg aaaaacctta tggtttcaaa ttcttttgct catctttttg ataatcaaac
      541 tttatcttgg tttggatgcg aggttgattt tattctatgg acatattttc attgtcttta
      601 atgccttaat gtatctatta tctagtagtc aggttagcct taaaaaaaac aaactgtctt
      661 cttaatctta cagcagtaga gcctgacaag gtgaggtcat agtagggagg atttctatga
      721 aaaagtatca acttctattc aaaataagtg cagtcttctc ttacttattt ttcgtatttg
      781 gtctttctca gctgacgctt atcgtccaaa actattggca attttcttct cagataggca
      841 atttattctg gattcaaaat atcttgagtt tactttttat tggagtcatg attgtggttc
      901 ttgttaagac aggccatggt tatctcttcc gcattccaag aaaaaaatgg ctttggtatt
      961 cgattttgac agtattagtg gtagtgttcc agatctcttt taacgttcag acagctaaac
     1021 atgttcagtc aactgcggaa ggttgggctg tattgattgg ttatagtggg actaactttg
     1081 cagagctagg tatttatata gccctgttct ttctggttcc actgatggaa gaattgattt
     1141 atagaggatt actgcaacat gctttcttta agcattcgcg atttggtctt gatttgcttc
     1201 ttccttctat tttatttgct ctccctcatt tttcaagcct gcctagtctg ttagatatct
     1261 tcgtctttgc aacagttgga atcatctttg ctggtttgac ccgctatacc aagagcattt
     1321 atccatccta tgcggtgcat gtgatcaata atattgtagc gaccttcccg tttttgctca
     1381 cttttctaca tagggtcttg gggtaaaaaa acaaaaggct tgcttttcag ccatagagga
     1441 ggtcatcatg tataaacact tatttttcct agattccaaa actttagatc ggttgacacc
     1501 ctatattcta gtcttggctt ctgacaccat tgcttttaat gtttttgtgc taacctttgt
     1561 atctgcggtg gtttttaatt tcctaaattc catgctagct ttaatggcta tattcatagg
     1621 ggctggctat gtggtcggat tttggttgct aaaatggttt gttttggaaa ggctagatct
     1681 aaagaatgac gtgtaggaag tctgtttgtt gaggaggata attttatgga gttttttgat
     1741 aaatttcatg ccttgtgttt tggattttta gtactaataa ttgtcattac agttccttat
     1801 acgattaacc atgggggttt ttttcaaaat gaatctgcat tgattcttgt aagtcttctt
     1861 gtaacctcgc tgagtgttgc ttatgctaga aagtttgaaa tgatttcttt tgggatgtta
     1921 agcaagaaac aacttttgct tttcattgca atctttcttc taagtgtact tgagacgctg
     1981 gtttatattc atttcttcgc tgtttcttct ggctcagggg tccaacactt ggcggaagtc
     2041 agcagaggaa tttccctgtc tttgatttta actacctcag tttttggccc catccaggag
     2101 gaactcattt tcagaggact tcttcaaggt gcggtttttg acaattcttg gttagggctt
     2161 gtgctaactt cctctctctt ttctttcatg catggacctt ctaatgtccc ttcgtttatt
     2221 ttttatctac ttgggggttt gttgctgggc tttgcttata aaaagagtca aaacctatgg
     2281 gtttctactc tagtccatat gctttacaac agttggccac tcttatatta tttataaaaa
     2341 ttatgaaaag gtaagcagaa gacggtgctt acctttttca agctataatg atacccaagc
     2401 caatccagag gcttttcttt gagaatcggg ttagagtggt tgacgaatag gccaaaaact
     2461 agtagaatag taaggaaact ttatacggag gaaagaaatg gatttgggtg ataatgagct
     2521 aacactgact cccatacctg ggaaaagtgg caaggcttat atgggtagct atcctgatgg
     2581 gaagcgcatc tttgtaaaaa tgaacacctc tccaatccta cctggtctag ctagagaaca
     2641 aattgctcca caattattat ggagtcgtcg tttggcagat gggcgtgata tgtgtgctca
     2701 agaatggttg acaggcaaga tattgacccc ctatgatatg aatcgtaagc aaatcgtcaa
     2761 tattttaacc cgtcttcatc gctcacgtcc gttgatgaca caattgagtc gtttgggcta
     2821 tgccatggaa acacctgtag atttactaca gtcttggcag gaaacggctc cagatgcttt
     2881 gcgtaaaaat cattttatca gtgaagtgat ggctgattta cgtcagacta ttccaggatt
     2941 tagagaggac catgcgacca ttgtccatgg agatgtacga catagtaatt ggattgagac
     3001 agatagtggc ttgatttatt tagtagattg ggattcggtt cgcttgaccg atcgcatgtt
     3061 tgatgtggcc catatgctct gccattatat ttcagaacat cagtggaagg aatggttgac
     3121 ctactacggt tacaagtaca atcaaacggt attaagtaaa ttgtattggt atggtcaatt
     3181 gtcttatttg agtcagattt ccaagtatta tatgaaccaa gatttagaaa atgtcaatcg
     3241 ggagattcat ggtttgcgtc atttccgaga caagtatgga aagagaagat gagagttaga
     3301 aatcgtaaag gggcaacaga attactagag gcaaatcccc agtatgtggt cctcaatccc
     3361 ttggaagcca aggcaaaatg gcgggacttg tttggcaatg ataatcccat tcatgtggaa
     3421 gttggaagtg gaaagggtgc ctttgtttca ggtatggcca agcaaaaccc tgacatcaac
     3481 tatatcggga ttgatattca aaagtctgtt ttgagctacg ctttggacaa ggtgcttgaa
     3541 gctggagtgt ctaacatcaa gctcttgtgg gtagatggtt ctgacttgac tgactacttt
     3601 gaagacggtg agattgatcg cttgtatctg aacttttcag atccatggcc gaaaaaacgc
     3661 catgaaaagc gtcgtttgac ctacaagacc tttttggata ccttcaaacg tatcttgcct
     3721 gaaaatggag aaattcattt caagacggat aaccgtggct tgtttgagta cagtttagtg
     3781 agcttttctc aatatggcat gaaactcaat ggtgtctggc tagatttgca tgccagtgat
     3841 tttgaaggca atgtcatgac agaatacgag caaaaattct caaacaaggg gcaagttatc
     3901 taccgagttg aggcagaatt ttaagagata gcctaaaatt aggctgtaca agtgcttttg
     3961 ctttacataa gttggcaaac gtgctatact gatagtaaga atatgaaaag tgaggcgggg
     4021 aaatatcttc gcctcttgct tatgaggagg tggacgcaat cgcaacaatc gtagaattag
     4081 tcagagaagt tgtagaacct gtcatagaag ctccttttga actcgtggat atcgagtatg
     4141 gaaagattgg cagtgacatg attctcagta tttttgtaga taaacccgaa ggaattacct
     4201 tgaacgacac ggcagacttg acagaaatga tcagtcctgt cctagacacc atcaagccag
     4261 atcccttccc agaacaatat ttcctagaaa ttaccagtcc aggtttggaa cgtcctttga
     4321 aaaccaagga tgccgtcgct ggagcggttg gaaaatacat ccatgtcggg ctctaccaag
     4381 ccatcgataa gcaaaaggtc tttgaaggaa ccttgttggc cttcgaagag gacgagttga
     4441 ctatggaata tatggacaag acgcgtaaga aaaccgtcca aattccatac agtttagtat
     4501 caaaagcacg tttagcagtt aaattataga aaaagaaagg atagcttttg aggattcaaa
     4561 agtgaagaaa acatgagtaa agaaatgcta gaggccttcc gcattttgga agaagacaag
     4621 ggaatcaaaa aagaagatat catcgacgca gtagtagagt cgcttcgttc cgcttatcgc
     4681 agacgctatg gtcagtcaga cagcgtagct attgacttca acgaaaaaac aggtgacttt
     4741 acagtttata ctgtccgtga agttgttgat gaagtatttg atagccgttt ggaaatcagc
     4801 ttgaaagatg ctcttgccat taattcagct tatgaacttg gagacaaaat caagtttgaa
     4861 gaagcaccag ctgagtttgg tcgtgtagca gcccaatctg ccaaacaaac catcatggaa
     4921 aaaatgcgca agcaaacacg tgccatcact tacaatactt acaaagaaca tgagcaagaa
     4981 atcatgtctg gtacagtaga acgctttgac aaccgcttta tctatgtcaa ccttggtagc
     5041 atcgaagccc aattgtcaaa acaagaccaa attcctggag aagtttttgc ttctcatgat
     5101 cgtatcgaag tttatgttta caaggttgaa gacaaccctc gtggtgtgaa cgtctttgtt
     5161 agccgtagcc atccagaaat gatcaaacgt ttgatggagc aagaaattcc agaagtttat
     5221 gatggaactg ttgaaatcat gagcgtggct cgtgaagcag gtgaccgtac gaaggttgct
     5281 gttcgtagtc acaatccaaa cgtggatgct atcggtacaa tcgttggacg tggtggtgct
     5341 aatatcaaga agattactag caaattccac ccagctcgtt acgatgctaa aaatgaccgc
     5401 atggtaccaa tcgaagaaaa tatcgatgtt atcgagtggg tagcagatcc agctgaattt
     5461 atctacaatg ccatcgctcc tgctgaggtt gaccaagtta tctttgatga aaacgacagc
     5521 aaacgtgcct tggtggttgt tccagataac aagctttctc ttgccattgg tcgtcgtgga
     5581 caaaacgtgc gcttggcggc tcacttgact ggttaccgta tcgatatcaa gtctgctagc
     5641 gaatttgaag ccatggaaga cgctgcttca gtagagttgg aagtagaaaa cgatactgta
     5701 gaagaataaa agctgctaga ggagggaaag atgaaaacaa gaaaaatccc tttgcgcaag
     5761 tctgttgtgt ctaacgaagt gattgataag cgtgatttgc tccgcattgt caagaacaag
     5821 gaaggacaag tctttattga tcctacgggc aaggccaatg gccgcggcgc ttatatcaaa
     5881 ctagacaatg cagaagccct agaggcgaaa aagaagaagg tctttaaccg cagctttagc
     5941 atggaagtgg aagaaagctt ttatgacgag ttgatcgctt atgtggatca caaagtgaaa
     6001 agaagagagt tgggacttga ataagcaaaa gataagtaat ctcttggggc ttgctcagcg
     6061 agcagggcgc atcatatcgg gtgaagaatt ggtggtcaag gccattcaag acggcaaggc
     6121 caagttggtc tttctagctc atgatgctgg acccaatctg accaagaaga ttcaagataa
     6181 aagtcattat tatcaagtag aaattgtaac cgtgttttca acactggaat taagcatagc
     6241 agtcgggaaa tcgagaaagg ttttggctgt aacagatgct ggatttacaa agaaaatgag
     6301 gtctcttatg gaatagaaga ggaggacatg atttgtctaa gaaaagattg tacgaaatcg
     6361 caaaagaact tggaaaagaa agtaaagaag ttgtagcgcg tgcaaaagag ttgggcttgg
     6421 atgtgaaaag ccactcatca agtgtggaag aagctgtcgc tgcaaaaatt gctgccagct
     6481 ttaagcctgc agctgctccg aaagtagaag caaaacctgc agccccaaaa gtaagtgcag
     6541 aaaagaaagc cgaaaaatct gagccagcta aaccagctgt agctaaggaa gaggcaaaac
     6601 ctgcagcccc aaaagcaagt gcagaaaaga aagccgaaaa gtctgaacca gtaaaaccag
     6661 ctgtagccaa ggaagaggca aaaccagctg agccagtcac tccgaaaaca gaaaaagtag
     6721 cggctaaacc gcaaagtcgt aatttcaagg ctgagcgtga agcacgtgct aaagagcagg
     6781 cagagcgacg caagcaaaat aagggcaata accgtgacca acaacaaaac ggaaaccgtc
     6841 agaaaaacga cggccgtaat ggtggaaaac aaggtcaaag caaccgcgac aatcgtcgct
     6901 ttaatgacca agctaagaag cagcaaggtc agcaaaaacg tagaaatgag cgccgtcagc
     6961 aagaggataa acgttcaaat caagcggctc cacgtattga ctttaaagcc cgtgcagcag
     7021 ccctaaaagc agagcaaaat gcagagtacg ctcgttcaag tgaggaacgc ttcaagcagt
     7081 atcaggctgc taaagaagcc ttggctcaag ctaacaaacg caaggaacca gaggaaatct
     7141 ttgaagaagc ggctaagtta gctgaacaag cacagcaagt tcaagcagtg gttgaagtcg
     7201 tccctgagaa aaaagaacct gcagtggata cacgtcgtaa aaaacaagct cgaccagaca
     7261 aaaatcgtga cgattatgat catgaagaag atggtcctag aaaacaacaa aagaatcgaa
     7321 gtagtcaaaa tcaagtgaga aatcaaaaga atagtaactg gaataacaac aaaaagaaca
     7381 aaaaaggcaa taacaagaac aaccgtaatc agactccaaa acctgttacg gagcgtaaat
     7441 tccatgaatt gccaacagaa tttgaatata cagatggtat gaccgttgcg gaaatcgcaa
     7501 aacgtatcaa acgtgaacca gctgaaattg ttaagaaact tttcatgatg ggtgtcatgg
     7561 ccacacaaaa ccaatccttg gatggggaaa caattgaact cctcatggtg gattacggta
     7621 tcgaagccaa acaaaaggtt gaagtggata atgctgacat cgaacgtttc tttgtcgaag
     7681 atggttatct caatgaagat gaattggttg agcgtccacc agttgttact atcatgggac
     7741 acgttgacca cggtaaaaca acccttttgg atactcttcg taactcacgt gttgcgacag
     7801 gtgaagcagg tggtattact cagcatatcg gtgcctacca aatcgtggaa aatggtaaga
     7861 agattacctt ccttgataca ccaggacacg cggcctttac atcaatgcgt gcgcgtggtg
     7921 cttctgttac cgatattacg atcttggtcg tagcggcaga tgacggggtt atgcctcaga
     7981 ctattgaagc catcaaccac tcaaaagcag ctaacgttcc aatcatcgta gctattaaca
     8041 agattgataa accaggtgct aacccagaac gcgttatcgg tgaattggca gagcatggtg
     8101 tgatgtcaac tgcttggggt ggagattctg aatttgttga aatttcggct aaattcaacc
     8161 aaaatatcga agaattgttg gaaacagtcc ttcttgtggc tgaaatccaa gaactcaaag
     8221 cagacccaac agttcgtgcg atcggtacgg ttatcgaagc gcgcttggat aaaggaaaag
     8281 gtgcggtcgc aacccttctt gtacaacaag gtaccttgaa tgttcaagac ccaatcgttg
     8341 tcggaaatac cttcggtcgt gtccgtgcta tgaccaacga ccttggtcgt cgtgttaaag
     8401 ttgctggacc atcaacacca gtctctatca caggtttgaa cgaagcaccg atggcgggtg
     8461 accactttgc cgtttacgag gatgaaaaat ctgcgcgtgc agcaggtgaa gagcgtgcca
     8521 aacgtgccct catgaaacaa cgtcaagcta cccaacgtgt tagccttgaa aacctctttg
     8581 atacccttaa agctggggaa ctcaaatctg ttaatgttat catcaaggct gatgtacaag
     8641 gttctgttga agccctttct gcctcacttc aaaagattga cgtggaaggt gtcaaagtga
     8701 ctatcgtcca ctcagcggtc ggtgctatca acgaatcaga cgtgaccctt gccgaagctt
     8761 caaatgcctt tatcgttggt ttcaacgtac gccctacacc acaagctcgt caacaagcag
     8821 aagctgacga tgtggaaatc cgtcttcaca gcattatcta caaggttatc gaagagatgg
     8881 aagaagctat gaaagggatg cttgatccag aatttgaaga aaaagttatt ggtgaagcgg
     8941 ttatccgtga aaccttcaag gtgtctaaag tgggaactat cggtggattt atggttatca
     9001 acggtaaggt tgcccgtgac tctaaagtcc gtgttatccg tgatggtgtc gttatctatg
     9061 atggtgaact cgcaagcttg aaacactata aagacgacgt gaaagaagtg acaaacggtc
     9121 gtgaaggtgg attgatgatc gacggctaca atgatattaa gatggatgat gtgattgagg
     9181 cgtatgtcat ggaagaaatc aagagataag attttttgct cctttcttag gtggtgaggg
     9241 acgcaagcaa accgatggtt tcattgctta tttttgagcc tagggtctca aaaatcccct
     9301 gtgatgggac tgataaatca gttccatcac tttcaccacg gcgaaagaag cagatgactt
     9361 caaattgaac ttcgtttcaa tttaaactga aaatcaagaa gtttaaaata gctaggtctg
     9421 ctggcctagc ttttggttca aagtagagaa aggaatatca tggcaaatca tttccgtaca
     9481 gatcgtgtgg gcatggaaat caagcgtgaa gtcaatgaga ttttgcaaaa gaaagtccgt
     9541 gatccacgtg tccaaggtgt gaccatcata gatgttcaga tgctgggtga cttgtctgtt
     9601 gccaaggttt attacaccat tttgagtaac cttgcttcgg ataaccaaaa agcccaaatc
     9661 gggcttgaaa aagcaactgg taccatcaaa cgtgaacttg gtcgcaattt gaaattgtac
     9721 aaaatcccag atttgacctt cgtcaaagac gagtccatcg agtatggaaa caagattgac
     9781 gagatgctac gcaatctgga taagaactaa agaagagggg ttgcccctct tttttggtgg
     9841 aggaaaatag gttgaatttg aaatggaaaa atattctttt ataatagatt gaaactagaa
     9901 tagtacgcct ctacttctaa aatattgtta gaaatcgatt tgactgtcct gatcgatttg
     9961 tcctgttctt gtttcatttt aatataaaaa agggattctg tattttttaa tgttatctaa
    10021 ttagaaaatg ctttttttgt aggaaatata atatgataag gtgcaaaaaa gaaataagga
    10081 gtttgtatat ggctgaacaa gacttagcta tgcaagtatt gcaacaagtg gtgaaactac
    10141 ctgttgttaa ggttgatcgt tcgaaatttt tagtggataa gttttccaaa gaattggatc
    10201 caaaagatat tcctacctta ttggaacaag gtccaacgac tcttctatct caagaaatat
    10261 tagatcgtgt agctaatgct tgtattcggg acaatgtatt attagcgagt gggacttctg
    10321 ttttggcagg attacctgga gggcttgcta tggcaattac cattccagct gatgtggctc
    10381 aattttatgc tttctctctg aaattggctc aagaattagg ttatatttat ggttatgagg
    10441 atctttgggc ttcacgagag gagttgagtg aagatgctca aaataccctc ttgctttatc
    10501 taggcgtaat gttaggggtg aatggaaccg ctgctttgct acgtgttggt agtataacaa
    10561 ttgccaaaca ggtaatgaaa atagtgccta ataaagcttt aacaaagacg ctttggtacc
    10621 ctattttgaa aaaagtctta aaaatatttg gtgtgaatct taccaaggga gggttggcca
    10681 aaggaatggg gaaatttatt cctatcttgg gtggtatcat ttcaggtggt ttaacctttg
    10741 caactatgaa accaatgggg gaaagcttgc agaaagaatt atccaagcta gtcaactata
    10801 gtgaagttca atatcaagaa gatgttgaaa caatccgaaa agaggctgaa atcatcaaag
    10861 gagagtaata tgaatcctat caaagctttt gctaaaattt atggtaatta ctttttgacc
    10921 gtgcaaggtg taaaagtgat gaaaacgata aagaaagctg accatgtcgt tgttggtctg
    10981 gggaaacttt ttattgccga caagttaatg gatacggctc ggtggctcat taagccagag
    11041 gagagagaat gaaatttttt tggtcttctt gctattcttt ttatcaaacc gattattggg
    11101 attgtgaaat tcttttggat gatcatctct tttgcagtcc aattgctgtt ttacaagata
    11161 gtgtttaaga tattggattg gctctttaaa cttatctaga tggtaatcca agttgcagag
    11221 aactagcagg aactccactg ctagtttttt attctctttc catatggtat aatataagca
    11281 gtaaaatcat tttatactct tcgaaaatct cttcaaacca cgtcagcttc accttgcagt
    11341 atatatgtta ctgacttcgt cagttctatc cacaacctca aaacggtgtt ttgagctgac
    11401 ttcgtcagtt ctatctacaa cctcaaaaca ctgttttgag caacctgcgg ctagcttcct
    11461 agtttgctct ttgattttca ttgagtatta gaacatacaa tggaggtcgt catggacaat
    11521 atcatcgatg tgtcaattcc tgttgcagaa gtggtggaca agcatccaga agtcttggaa
    11581 attctagtgg agttgggttt taaacccctt gccaatccct taatgcgcaa tacagttggt
    11641 cgtaaagtat cacttaaaca gggttctaag ctagcaggaa ctcctatgga caagattgta
    11701 cgcacactgg aagcgaatgg ctacgaagtg attggattag actaatgaca gatgaacgga
    11761 ttcatatcct acgggatatt ttgttagaat tgcacaatgg cgcctctcct gagtcggttc
    11821 aagatcgctt tgatgcgacc tttacgggcg tgtcagccat cgagatttcc cttatggagc
    11881 acgagctgat gaactcggat tcgggcgtca cttttgaaga tgttatggaa ctctgtgatg
    11941 tccatgccaa tctttttaaa aatgctatca aaggtgtcga agtttcagat actgagcatc
    12001 caggtcaccc agttcgtgtc ttcaaagaag aaaatctggc tctccgtgcg gccttgattc
    12061 gcattcgtag attgttagat acctatgagt ctatggaaga cgaggaaatg ctggcggaga
    12121 tgcgtaaggg tttggtgcgt cagatgggac ttgtgggtca atttgacatc cattaccaac
    12181 gtaaggaaga actcttcttt cctatcatgg agcgctatgg acacgattca cctcccaaag
    12241 ttatgtgggg agtggatgat cagattaggg aactctttca aacagctcta acgacagcca
    12301 agtcactacc agaagtgtca attagcagtg taaaggaagc ttttgaagct tttgcgacag
    12361 agtttgaaag tatgattttc aaggaagagt ccatcctcct catgattctc cttgagtctt
    12421 ttactcagga tgactggctt cagattgcgg aggagagcga tgcctatggc tatgccatca
    12481 tccgtccgtc agagaaatgg gtgccagaac gacagagctt tattgaggaa aagattgcag
    12541 aggagcctgt acagctagat acggcagaag gtcaagttca acaagtcata gatacgccag
    12601 aaggccattt taccattacc tttaccccta aggaaaagga agctgtgctg gaccgccata
    12661 gtcaacaggc ttttggtaat ggctatcttt cagtcgagca ggccaatctc atcctcaatc
    12721 atctccctat ggagattacc tttgtcaata aagaagatat tttccagtat tacaatgaca
    12781 atacgccagc tgatgagatg attttcaaac ggacgccgtc ccaagtcggg cgcaatgtcg
    12841 aactctgcca tccgcctaag tacttggaca aggtcaaaac tatcatgaag gggcttcgtg
    12901 agggaagcaa agacaagtat gaaatgtggt tcaagtctga gtcgcgaggt aagtttgtcc
    12961 acatcaccta tgctgcagta cacgatgaag acggagaatt ccaaggagtg ttggagtatg
    13021 ttcaggatat ccagccctac cgtgagattg atacggacta ttttcgtgga ttagaataag
    13081 gagaaaaaat gagttacgaa caagaattta tgaaggaatt tgaagcttgg gtcaataccc
    13141 aaatcatgat taacgacatg gcgcacaagg aaagccaaaa agtttacgaa gaagaccagg
    13201 acgagcgtgc caaagatgcc atgattcgct acgagagtcg cttggatgct tatcagttct
    13261 tgcttggtaa gtttgaaaac ttcaaagtag gcaagggatt ccatgatttg ccagaaggct
    13321 tgtttggtga gcgaaattat taaacgagaa agattcttga tttttcacta aaatcttgat
    13381 agaatgttta tgttaaatcc ttgtcagagc agggattttt tattgaaagg attttatcat
    13441 gtcaaagaaa ctcaatcgta aaaaacaatt acgaaatggc ctccgtcgcg caggtgcctt
    13501 ttcaagtacg gtgactaagg ttgtagatga gacaaaaaaa gtcgtgaagc gtgcagaaca
    13561 gtcagcaagc gcagctggta aggctgtttc taaaaaagtt gaacaagcag tagaagctac
    13621 caaagagcaa gctcaaaaag tagctaattc tgtagaagat tttgcagcaa atttgggtgg
    13681 acttccactt gatcgtgcca agactttcta tgatgaagga atcaagtctg cttcagattt
    13741 caaaaactgg actgaaaaag aactccttgc cttgaaagga atcggcccag ctaccatcaa
    13801 gaaattgaaa gaaaatggca tcaagttcaa gtaatttttc ttgagccttg catttccgaa
    13861 aaaatcttgc tacaatagag ccattagagg tgttttgaat cccacatttt acagaaagtg
    13921 gcggcgctga gaagtccaca aatgtgtcaa aactggttgc taatggatga aaaattgaaa
    13981 taaaagtgtc tttttgcttt aaagacgaga gttgcgggca actgcccgaa attgggtggt
    14041 accgcggata aacacattcg tccctgtcat gtagatggca gggcttttct tttacgtctt
    14101 agtcaaaggg ggttgttatg aagcaatctt ttaacacaag taaactatat tatggttttc
    14161 ctgtttttat tttaggatat caagattaga attttggcta taacatcacg acctgtagtt
    14221 cctcttatag tctaggagat tgggttgtga ttggagtcgt tgcgagagag aatgccgcag
    14281 agcagatcaa acagtatcaa aaatttactg tgaatatttc tgatgaaact tctatgcttg
    14341 cgatggagca ggctggtttt atcagtcatc aggagaaatt ggaacgtttg ggagtgcatt
    14401 atgaaatttc tgaacgaact cagactccta ttttagacgc ctgtccactt gttttagatt
    14461 gtcgggtaga taggattgtt gaggaagacg gtatttgcca catctttgcc aagattcttg
    14521 agcgacttgt tgccccagaa ctcctggatg aaaagggaca ttttaaaaat caactgtttg
    14581 ccccaaccta tttcatggga gatggatatc agcgcgttta tcgctatctg gacaagcgtg
    14641 tagatatgaa gggcagtttc atcaaaaaag cgaggaagaa ggatggcaag aactgagctg
    14701 ccagataaaa tcgaaacaga acgtctcgtt ttacgagtcc gcacagtggt ggatgctgag
    14761 gatatccata cctacgctag tttgccagag gtcgcctatc cagcaggttt tcctccagtc
    14821 aagatcttgg aagatgagat ttattatctg gagcacattc ttccggagcg tactcaaaag
    14881 gaaaatctcc cagctggcta tgggattgtc gtcaaaggaa ccgataaagt cattggttct
    14941 gttgacttca accatcttca tgcggatgat gtgctagagc ttggctatac cttgcaccca
    15001 gactattggg gtcgaggtta tgttccagaa gcagcgtgtt ccttgattga tctagctttt
    15061 aaagaacttg gtcttcacaa gattgaacta acttgctttg gatataacct tcaaagtcaa
    15121 cgagtctcgg aaaagcttgg ctttaccctc gaagctcgca taagagaccg caaagatgct
    15181 caaggcaact gctgtgatga tttgagatat ggcttgctga ggagtgagtg ggaggtgatt
    15241 tgatgcatct tttcatcatt ggtgctccag catcaggaaa aatgaccatt ggtcaagaat
    15301 tatctcgact gacggatgct accctatttt ataaccatca agccattgat tttgcactag
    15361 agatctatca ggattatacc gaggagatgt gggaatttgt tcgtggaatt accttttctt
    15421 tccttggagc aagtgcaaga aatcagcgat cagtaatttt aacagacgta attgattttt
    15481 caaatcagta ccagctgatg tatttaaagc aaattcagga tttattggat aactttcatc
    15541 aagagattct gtttgttgaa ttggaaacaa gtcttgaaga gtgcatacgt cgaaatcgaa
    15601 cggagaatcg attaaagcac aaacccttaa aacgacatat tgaggtatct gaaagagaaa
    15661 ttttagagac cgctgaaaca cttcaattaa attcccaaca tcaaccgaat gagttgcacc
    15721 actactttaa aataaataat acgaatttgt ctgcagaaga agctgctaag caaattcaaa
    15781 ataaaataaa caaaatagag aaaggacaca cacatgtcta aagaactttc acctaaatac
    15841 aatccagccg aggttgaggc tggtcgttac caaaaatggc ttgatgcgga tgttttcaag
    15901 ccttcaggcg atcaaaaggc taagccttat tcaatcgtga ttccaccacc aaacgtaact
    15961 gggaaacttc accttggtca cgcttgggat acgactttgc aggatatcat catccgtcaa
    16021 aaacgcatgc aaggctttga taccctttgg cttccaggta tggaccacgc tggtattgcg
    16081 actcaggcta aggttgagga gcgcttacgt ggtgagggta ttacgcgtta tgacctaggt
    16141 cgtgagtctt tcttgacgaa ggtctgggaa tggaaagacg aatatgccac taccatcaag
    16201 gaacaatggg gcaagatggg gctttctgta gactattctc gtgagcgttt cactcttgat
    16261 gaaggtttgt caaaagctgt tcgtaaggtc tttgtgaacc tttacaagaa aggctggatc
    16321 tatcgtggtg agtttatcat caactgggac ccagcagctc gcacagccct ttctgatatt
    16381 gaggtgattc acaaggatgt agaaggtgcc ttctaccaca tgaattacat gctggaagat
    16441 ggttcacgcg cccttgaagt tgctacaact cgtcctgaga ctatgtttgg ggacgttgcg
    16501 gttgcggtta atccagaaga cccgcgctac aaggacttga ttggtaaaaa cgtcatcctt
    16561 ccaatcgcta ataaactcat cccaatcgtt ggagatgagc acgcagatcc tgagcttggt
    16621 actggtgtcg tgaaaatcac acctgcccac gatccaaatg acttcttggt tggccaacgt
    16681 cataacttgc cacaagtcaa cgtcatgaac gacgacggaa ctatgaatga gcttgccttt
    16741 gaattttcag gcatggatcg ttttgaagct cgtaaggcag tcgttgctaa gttggaagaa
    16801 atcggtgccc ttgtcaaaat cgaaaaacgt gtccacagtg ttggtcactc agaacgtaca
    16861 ggtgtcgtag ttgagccacg cttgtctact caatggttcg tcaagatgga ccaattggct
    16921 aagaacgcca ttgctaacca agacacagag gacaaggtcg aattctaccc acctcgtttc
    16981 aacgatacct tccttcaatg gatggaaaat gtccacgact gggttatctc tcgtcagctt
    17041 tggtggggtc accaaatccc tgcctggtac aatgctgatg gtgaaatgta tgtcggcgaa
    17101 gaagctccag aaggtgacgg atggactcag gacgaagacg tcttggatac ttggttcagt
    17161 tctgccctct ggccattttc aaccatgggc tggcctgaag tcgactcaga agactttaaa
    17221 cgttatttcc caacttcaac cttggtaaca ggttacgaca tcatcttctt ctgggtgtct
    17281 cgtatgatct tccagtcctt ggaatttact ggtcgtcagc cattccaaaa cgtgcttatc
    17341 cacggtctca tccgtgacga gcaaggacgc aagatgtcta aatctctcgg taacgggatt
    17401 gacccaatgg atgtcatcga gaaatacggt gctgatgccc ttcgttggtt cctttcaaac
    17461 ggttccgcac caggtcaaga cgttcgtttc tcttacgaga aaatggatgc ttcatggaac
    17521 tttattaaca aaatttggaa catttctcgc tatatcctca tgaacaatga aggtttgacg
    17581 ctggatgtgg cgcatgacaa tgtcacaaaa gttgcaacag gtgaggctgg taatgtgacg
    17641 gaccgctgga ttctccacaa tctcaacgaa accattgcaa aagttactga aaactttgat
    17701 aagttcgagt ttggtgtggc tggtcatatc ctttacaact tcatctggga agaatttgcc
    17761 aactggtatg ttgagttgac caaggaagtt ctatacagcg acaatgaaga cgataaggtc
    17821 attactcgat ctgttctcct ctatacgctg gacaagatcc ttcgtctcct tcacccaatc
    17881 atgccattcg tgacagagga aatctttgga caaatctcag aaggttctat cgtgacagct
    17941 gcatacccaa ctgttaatct agcctttgaa gaccttgcgg ctcacactgg tgtggaaagt
    18001 ctcaaagact tgattcgtgc tgttcggaat gcgcgtgcgg aagtaaatgt agcaccaagc
    18061 aagcctatca ccatccttgt taagacaagc gatagcgact tggaagcctt ctttaacagc
    18121 aatgtcaact atatcaaacg ctttacaaat ccagaacact tggaaatcgc atcaaccatc
    18181 cctgcacctg aactggctat gtcaagtgtc atcacaggag cagaaatcta cctgccactg
    18241 gccgatctcc tcaatgttga agaagaactg gcccgtctcg acaaggaact tgctaaatgg
    18301 caaaaagaac tggacatggt tggtaagaaa ctctctaacg aacgcttcgt agccaacgcc
    18361 aaaccagaag tcgtccaaaa agaatgcgac aaacaagctg actaccaagc taaatatgac
    18421 gcgaccgtcg cacgtattga tgagatgaag aagttggtga aatgatgtta aaaacctaac
    18481 cctatcgggt tgggttttac atttgataat gttataataa aaaggagaaa ctttaggaga
    18541 atactatggg gaaaattgtt gctattgatt tatttaacgg tgcaggtggc actacaagtg
    18601 gccttaaaaa atcaggcata gatgttcaag ttgctgttga aattgattcc gtagctgtta
    18661 aaacttataa gttaaataat cctgaggtgt ctgtaataga tatggagtaa ataaaacttc
    18721 aattttacaa gctttatatg gttctccagc caataaaagt gtgtctgaat attggtttag
    18781 tacaaatgtt gatcaaatag atgaagtgga tgggaaacaa tgtttaatct attcatatta
    18841 taacattaaa gcttctagaa atgtagaagt tcttaaaggc cgttctggaa caaaaaaagg
    18901 attagattat tgggaaccat atgctcctca aaaacaatat gagatggaaa gattacccaa
    18961 aaataaatat attggaagtt cttcgacaga cagatgggat ggcattgaaa aaaa
//
LOCUS       DAVHEW010000033        18685 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVHEW010000033 DAVHEW010000000
VERSION     DAVHEW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.033"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(229..1035)
                     /gene="yidA"
                     /locus_tag="VVL53_001052"
     CDS             complement(229..1035)
                     /gene="yidA"
                     /locus_tag="VVL53_001052"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU4183239.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1048..2352)
                     /locus_tag="VVL53_001053"
     CDS             complement(1048..2352)
                     /locus_tag="VVL53_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4183240.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2424..2801
                     /locus_tag="VVL53_001054"
     CDS             2424..2801
                     /locus_tag="VVL53_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU4183241.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2892..3224
                     /locus_tag="VVL53_001055"
     CDS             2892..3224
                     /locus_tag="VVL53_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU4183242.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3236..4807
                     /gene="ffh"
                     /locus_tag="VVL53_001056"
     CDS             3236..4807
                     /gene="ffh"
                     /locus_tag="VVL53_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU4183243.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5002..6285)
                     /locus_tag="VVL53_001057"
     CDS             complement(5002..6285)
                     /locus_tag="VVL53_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU4183244.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6588..7301)
                     /gene="rsmG"
                     /locus_tag="VVL53_001058"
     CDS             complement(6588..7301)
                     /gene="rsmG"
                     /locus_tag="VVL53_001058"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU4183245.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7395..7955
                     /locus_tag="VVL53_001059"
     CDS             7395..7955
                     /locus_tag="VVL53_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU4183246.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7957..8856
                     /gene="htpX"
                     /locus_tag="VVL53_001060"
     CDS             7957..8856
                     /gene="htpX"
                     /locus_tag="VVL53_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU4183247.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8900..10459)
                     /locus_tag="VVL53_001061"
     CDS             complement(8900..10459)
                     /locus_tag="VVL53_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4183248.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLSKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10698..11015)
                     /locus_tag="VVL53_001062"
     CDS             complement(10698..11015)
                     /locus_tag="VVL53_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU4183249.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FEF"
     gene            11185..11727
                     /locus_tag="VVL53_001063"
     CDS             11185..11727
                     /locus_tag="VVL53_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU4183250.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11727..12356
                     /gene="nth"
                     /locus_tag="VVL53_001064"
     CDS             11727..12356
                     /gene="nth"
                     /locus_tag="VVL53_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4183251.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12567..13088
                     /gene="pyrR"
                     /locus_tag="VVL53_001065"
     CDS             12567..13088
                     /gene="pyrR"
                     /locus_tag="VVL53_001065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU4183252.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13107..14030
                     /locus_tag="VVL53_001066"
     CDS             13107..14030
                     /locus_tag="VVL53_001066"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU4183253.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14080..15159
                     /gene="carA"
                     /locus_tag="VVL53_001067"
     CDS             14080..15159
                     /gene="carA"
                     /locus_tag="VVL53_001067"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU4183254.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15471..18647
                     /gene="carB"
                     /locus_tag="VVL53_001068"
     CDS             15471..18647
                     /gene="carB"
                     /locus_tag="VVL53_001068"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU4183255.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTRLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAIAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5435 a         3598 c         4205 g         5447 t
ORIGIN      
        1 agagtattta aaatatcttg ggaaattata atttctccct gttttttgag tccaatctat
       61 gagtatgagt tgcccaggag taaggccaca ttctaattcg tttaggtaaa aattgttaca
      121 atcagaattt gtgtctctat tatgattatt ttgttttaaa ttaaatagtt tgctaaaaaa
      181 attcattgga ttttattgct aatcaattct tattgaaaaa tgatactttt acagtaccca
      241 tgttcggatg gcatgggcaa cgccggattc gtcatttgtt ttggtgatgt atttggcgat
      301 ttttttgatt tctggatttc cattttccat gacaacgggg tttccaacga cttccagcat
      361 ggcacggtca ttttcttcat caccgattgc catggtttca tctttggtca atccgagttt
      421 ttcagccaag tgagtaatgg ctgaaccctt gtctacattc tttttaagga gttcgaggta
      481 gaaaggagca gatttgttga tggagtagcg ctcgtaaaat tctgctggaa ttttttcaat
      541 cgcagcatcg agaatttctg gttcatcgat aaacatacat ttaacaattt ctttgccagc
      601 catttcttca ggggtacggt agaagatagg catgctgacg agggttgatt cgtgtacagt
      661 gtattttccg atattgcgat ttgcagtata gataccgtcc ttggtaatgg catgcatgtg
      721 gacaccgagc ttgcgactga ggaattccat atctagataa tcctcataag tcaaggattc
      781 gctgataatc tcatgtcctg tagcagtttc ttggacaagg gcaccgttga aggttaccac
      841 atagtccccc tcgtctctca actgcaagtc gtctagaagt ttggcaacgc ctgcgatagg
      901 gcggccagtt gcaatcacga ctttgacacc agcttctttg gcatcttgga tggcagaaaa
      961 aacttcagga gtgatttcct tttggctgtt gacaagggtt ccgtcgatat caacggcaat
     1021 tagtttaata ctcataaatt tcctcttcta gtttttattt ggggtaaaat gatcgttctc
     1081 aatcaagtgt aaaaattgct gggtaatgct tgcaaagatg ctgttttggt ccaacatttc
     1141 ttttggaaaa taaaagcgat tatctccgtg gcgactgcca gcaagggatt ggacgatagg
     1201 agacaggcta gagagttcgg ccagttctcc atttttttgt aaaatctcaa tctgtgtccg
     1261 tgggttttca gattcgggac gatagatatc ataagggagg tcaaagttct tatgaatggc
     1321 agtgtagtag tcgggatcaa agccgatatc ctcaaccaat tttctcatgc tagtaagttg
     1381 atcttggtcc tcttgtgaaa aggtaatgga tttaaagacc ttgcggttga caaagcgatg
     1441 cgataaatct gcaagaatct tgtcaggact ggtcatccaa agctggaagt aggtattcat
     1501 cacgccatca tccagagcca gatagtcagt caaggtcaca tttttttcga agaaaggcag
     1561 gaggtgtgga gaagttcggg caaagaaatc cttgtcctca ggatagagtt ccttggcgcg
     1621 tttgagaaga ttctgtagga gaacttccat ggcgcgtgtt gcggggtgga aataaacctg
     1681 catgtacatc tggtagcgac tgaggacgta gtcttcgatg gcgtgcatgc cattgcgctg
     1741 aaaggcgata ccattttcga taggacgaat gactcggagg attcgagtca ggtcaaattc
     1801 cccataggat gctcctgtaa aataggagtc gcgcaagaga tagtccatgc gatctgcgtc
     1861 aatctgacta gaaatgagct gcacgacctg cttattagga taggtatggt caatgacact
     1921 ggccaccttt tctgggaaat caggtgccac ttgtagcagg acttggtgaa tctctgtctc
     1981 aggattttga ataatctcct gagtaatggc ttcatggtct gtatcaaaga gatgttcaaa
     2041 agtatgggag taggcaccat gcccaaggtc gtgtaggaga gcagcggtca tggtcaagag
     2101 agactcggca ggattccatt cctcaggata tttttcttcg aaaatctctg tgatgcgtcg
     2161 tgcaatttca tagactccta gacagtgaga gaagcgactg tgttctccac cgtggaaggt
     2221 ataactggaa gttcccagtt gtttgatccg gcgcaaacgc tgaaattctt ttgtattaat
     2281 caagtcatag atgatttgat tattgacatg gatgtagttg tgaacagggt cacggaatac
     2341 tttttcgttc atatgaccat tatagcaaaa tcctgctctt tttggtaaaa tagtaggaca
     2401 agagacaaga aagagggcct gatgtgaaga ttcggatgcg aaatacgatt cagtttgatg
     2461 agcagttgga agtgattgac cagctttatg atgtggaagt gcatgaaaaa ggagattata
     2521 gctacctgct tttctataat gaggaaaagg aaaaagtagt gattaaattt catggtcaag
     2581 aacttgtgat gagccgattt tctaatccca agaccattat gcgctttcta aaggatagcg
     2641 atagtttagc ctatattcct acacctatgg gcatgcagga gtttatcatc caaacgagcc
     2701 attatcaagt tgataggcaa aagattgaac tagattatca actacaaaat caagagggac
     2761 atccctttgc cagctatcaa ttggaaatta cttggggcta ggctcatgtc tttttcaaat
     2821 ttagctatct atcttcttgg atcggcttcc tagtgtggta gattaggcta atttttggta
     2881 taataaaagt tatggaaatc gaaaaaacca atcgtatgaa tgcgctcttt gaattttatg
     2941 cggcgctttt gacagataag caaatgaatt atatcgagct ctactacgct gatgattaca
     3001 gccttgctga aattgccgag gagttcggtg tcagtcgtca ggctgtctat gacaatatca
     3061 agcgaacaga aaagattctg gaagattatg agatgaaatt gcacatgtac tcggactata
     3121 ttgtccgcag tcagattttt gatcagattt tggagcgcta tcccaaggat gactttctgc
     3181 aggagcagat agaaatttta acaagcattg ataatagaga ataagaggaa gaaaaatggc
     3241 atttgaaagt ttaacagaac gtttgcagaa cgtctttaaa aatctacgta aaaaaggaaa
     3301 aatctctgaa tctgatgtcc aagaggcaac caaagaaatt cgcttggcct tgctcgaggc
     3361 cgacgttgcc ttgcctgttg taaaggactt tatcaagaaa gttcgtgagc gtgcagtcgg
     3421 gcatgaggtc attgatacac ttaatcctgc gcaacagatt attaaaatcg ttgatgagga
     3481 actgacagcc gttttaggtt ctgatacggc agaaattatc aagtcaccta agattccaac
     3541 catcatcatg atggttggtt tacaaggggc tggtaaaaca acctttgctg gtaaattggc
     3601 caacaaactc aagaaagaag aaaatgctcg tcctttgatg attgcggcgg atatttatcg
     3661 tccagctgcc attgaccagc ttaagacctt gggacaacag attgatgtgc ctgtctttgc
     3721 acttggaaca gaagtaccag ctgttgagat tgtacgtcaa ggtttggagc aagcccaaac
     3781 taatcataac gactatgtct tgattgatac tgcgggtcgt ttgcagattg atgagctcct
     3841 catgaatgag cttcgtgatg tgaaagcatt ggctcaacca aatgaaatct tgcttgtcgt
     3901 tgatgctatg attggtcagg aagcagccaa tgttgcgcgt gagtttaatg ctcagttgga
     3961 agtgactggg gtcatcctta ccaagattga tggcgatact cgtggtggtg ctgctctgtc
     4021 tgttcgtcac attactggaa aaccaatcaa gttcactggt acaggtgaaa agattacgga
     4081 cattgaaacc ttccacccag accgcatgtc tagccgtatc cttggtatgg gggatatgct
     4141 cactttgatt gagaaagctt ctcaggaata cgatgaacaa aaagcccttg aaatggctga
     4201 gaagatgcgc gaaaacacct ttgattttaa tgatttcatc gatcaattag atcaggtgca
     4261 aaatatgggg ccgatggaag acttgctcaa gatgattcca ggtatggcca acaatccagc
     4321 ccttcaaaac atgaaggtgg atgaacgcca gattgctcgt aaacgtgcca ttgtgtcttc
     4381 gatgacacct gaagagcgtg aaaacccaga tttgttaaat ccaagccgtc gccgtcgtat
     4441 tgctgctggt tctggaaata cattcgtcga agtcaataaa ttcatcaagg actttaacca
     4501 ggctaaacag ctcatgcagg gtgttatgtc tggggatatg aataaaatga tgaagcaaat
     4561 ggggattaat ccaaataacc ttcctaaaaa tatgccaaat atgggaggaa tggatatgtc
     4621 tgcccttgaa ggaatgatgg gacaaggcgg tatgcctgac ttatcagctc tcggaggagc
     4681 aggaatgcca gatatgagcc agatgtttgg tggcggtttg aaaggtaaaa ttggtgaatt
     4741 tgccatgaaa cagtccatga aacgtatggc taacaaaatg aagaaagcga agaagaaacg
     4801 caagtaagac aagagattga tgcaaatctc atttgatgag aaaataaagt aaaaatttct
     4861 atgataaaac gtacagtatc aaggttttag aatatctgat attatacgtt ttttgatttt
     4921 aataattttg ttaagacact tttattatgg tatacgaaaa atccactcag ctgtctgagt
     4981 ggattaggtg tatttagact cttagtcttt attttcgtat ggcaagatca agttcaagat
     5041 gattcctgtc atggctgaaa gggcagtacc tgaaagtgta actggaccaa gtttaaggat
     5101 agctcctcca agtccaagaa ccaacatagc acttgcgatg atgaggtttc gcatttgagc
     5161 gaaatcaaca cgttctttaa tcaagacttt caaaccattg ctggcgataa ccccatagag
     5221 aaggattgac ataccaccaa gtacagcgtt tggaatagtt gaaatcaagg cagtgaattt
     5281 accaaggaag ctgagggcaa tcgcgatgaa ggcagcgtta cggataactg agacagaagc
     5341 gatacgagtc ataccgataa cccctgtatt ttctccgtaa gttgtattgg ctggtccacc
     5401 aaggaaggca gaaacagaag ttgcgatacc gtcaccaaga agagtacggt gaagacctgg
     5461 ttcttttaag aattgacgac cacagatttg acccaaaaca gtatggtctc cgatatgttc
     5521 agaaattgtt acgatagcga ttggcaagat agcgatggct tctggaccaa agtaaagatt
     5581 gtactcttta aaggcaccac ctgtgctaaa tggcaagtag aaaccaggaa tttcgaacca
     5641 gttggcttta agaactggtg taaagtcaac caagccaaga gttagtgcga aaaggtaacc
     5701 accgataatg gcaaagagga atggaatgat tcgtaggaag ccttttcctt ttgtattgat
     5761 aaaggcagca attaggaaag taacaacggc taccagagca tttttccaat ttccgtctgc
     5821 tacaagacct gcattggtaa cagctgaacc tgcaagtcca agaccgataa cgatgatcat
     5881 aggaccgata atgattggtg gcaagagttt atcaatccat tttgttccta caaatcggat
     5941 gctggtagca acaaggacat agaccaaacc agtcaagata acccctgttt gggcagcaga
     6001 tacatccccc cccatttctt tcatagccag tgacatagct gtgataaagg caaatgaaga
     6061 acctagataa actggaactt taaaaccagt agcaatcatg tagatgagtg ttccaacacc
     6121 tgaagcaaaa agggcaacag atacaggcat tcccaaaatc aatggtacca agatggtcgc
     6181 accaaacatg gcgaaaacgt gttggaaact aaggagaatt ccttttccag ccgaaggacg
     6241 ttggtcaacg tctagtaaca aatcaacagt tgattcttgt ttcatataaa cctcactttt
     6301 gggcatcaaa aaagagccca ttattttaca gcaataggcc ctcagagtaa gacttcttta
     6361 aaatttagat tttaaagagt tttaaatatt tctgcgtctt cgtcacctca cgggatgaca
     6421 ttaaaaacct ttgcttagga tagtatagca gaaaaaaatg attttgtaaa tcattttttc
     6481 ccgagcctag aaataaaaga gcgaggctga ttttgtaaat cattttttcc cgagcctaga
     6541 aataaaagag cgaggctgat tttgtaaaca tttgtttact aaaaaattta aagtgggcgt
     6601 ttatttggca taccagcctt acgcggatat ttatttggtg tttctttttt cttttctacc
     6661 actgtgatat agcgcggatc tctattcggt agggcgtagc tgagattgtc ttcgacctta
     6721 ctaaaaagga gattgagggc attcttagct tctaataatt cctcaggcgc attgctggcc
     6781 ttgagtgcca atagttttcc accaacctta aggtagggaa tggtcaattc agataggacc
     6841 tgcatacggg caaccgcacg agctgttaca aaatcatatt gagcacggaa gttcttgtct
     6901 tgggcaaaat cttcggcacg tccgtggtag aaatgaactc cgttcaaatc cagttcttga
     6961 gccaagagtt gtaggaagtt gatgcgctta ttgagtgaat caataatggt cacatctaac
     7021 tccggataga gaattttcat tggtagacta ggaaatcctg ccccagcccc gatatcaaga
     7081 agtttgatag tttcattggg aatcaaacct tgaagaatgg gtgcaatcga atcgtaaaaa
     7141 tgtttgagat aaacttcttc cttgtccgta atcgccgtca aattaatctt ctcattccac
     7201 tcgaccaaga gctcaaaata acgttcaaat tgttcttttt gctggtccga aagtggaaga
     7261 ttctgctcgg caagcaagtt gtaaaatgtt tttggtttca tagttatttc cttctttagt
     7321 atcatcttat tttaccacaa tcatagaaaa tttgatatac tggtactaat ctaaaagcag
     7381 aaaggactta tattatgact tggattattc ttggagttat cgctcttatt gttatttttg
     7441 tgattgttag ctataacggt ttggttaaaa atcgtatgca aaccaaggag gcttggagtc
     7501 agattgatgt tcagttgaaa cgtcgaaatg acctcttgcc aaacttgatt gagactgtaa
     7561 aaggttatgc caaatatgaa ggttctaccc ttgaaaaggt ggcagaacta cgtaaccaag
     7621 tggcggcagc gacttcacca gcagaagcta tgaaagccag tgatgccctc actcgtcagg
     7681 tttcaggtat ttttgcagtt gcagaaagct atccagattt gaaagctagt gctaactttg
     7741 ttaaattgca agaggagttg acaaacacag aaaataaaat ttcttactct cgtcaactct
     7801 ataacagtgt tgtcagcaac tacaatgtaa aattagaaac tttcccgagc aatattatcg
     7861 ctggaatgtt tggatttaaa gcggcagatt tccttcaaac acctgaagag gaaaagtcgg
     7921 ttcctaaagt tgattttagc ggtttaggtg actaagatgt tgtttgatca aattgcaagc
     7981 aataaacgaa aaacctggat tttgttgctg gtatttttcc tactcttagc tcttgttggt
     8041 tatgcggttg gttatctctt tataagatct ggacttggtg gtttggttat tgcactgatt
     8101 atcggcttta tctacgcttt gtctatgatt tttcaatcga cagagattgt catgtccatg
     8161 aatggagcgc gtgaggtgga tgagcaaacg gcaccagacc tctaccatgt agtggaagat
     8221 atggctctgg tcgctcagat tcctatgccc cgtgttttca tcattgatga tccagcctta
     8281 aatgcctttg cgacaggttc taatcctcaa aatgcggctg ttgctgcgac ttcaggtcta
     8341 ctagctatca tgaatcgtga agaactagaa gctgttatgg gacatgaagt cagtcatatt
     8401 cgtaattatg atatccgtat ttcgactatt gcagttgccc ttgctagtgc tatcaccatg
     8461 ctttctggta tggcaggtcg tatgatgtgg tggggtggag caggtcgcag acgaagtgat
     8521 gatgaccgag atggaaatgg tcttgaaatc attatgctag tggtttccct actagctatt
     8581 gtactggcac ctctcgctgc aaccttggtt cagctcgcta tttctcgtca gagggaattt
     8641 ctggcagatg catctagtgt tgagctgact cgcaatcccc agggaatgat taatgcccta
     8701 gataagttgg acaatagcaa acctatgagt cgccacgtcg atgatgctag cagtgccctt
     8761 tatatcaatg atcctaagaa aggtgggggg ttccaaaaac tcttttatac ccacccacct
     8821 atctcagaac ggattgaacg tttaaaacag atgtaaaaga atccagaggt cgtctctgga
     8881 tttttgctat gttgcaaatt tataaatctt ctaaattaac tagcttcaaa ccgtgttctg
     8941 tcatgcgata gatgatcgag cagacttctt ttaagaaacg acggtcatgc gaaacagtga
     9001 tgagaccgcc tggataggtt gcaaagagtt ttctgatttg gggttgagaa gtgggagaaa
     9061 agtttcgtgt gggttcatcc agcaggagaa agtttggttt gcgcaggact aaatccaaaa
     9121 gcaggagttt tccctgttgt ccgccagata aggagcgaat ttgatgctgc atttctggat
     9181 aactgaaatt gagactggct aggtgagagt ggattttctg aagttcctct ttttccccag
     9241 ttttgctgat ataagctatt ggtgataaat ccaattgtag ttttttgtgg taatcttgtg
     9301 gcataaaacc aagggaaatc tctcttttat cattcagaag ttgctttaat ttggctaaca
     9361 gagttgattt cccaacacca tttggcccga taataccgat tttttcttgc ccacggacag
     9421 ttagttgtag tttttgaacc aaaactcggt cgtcaatgga caaattttct ttttccagtt
     9481 ggactaagac tttagaagct ggtaatggtt ggatgtctga aaagaaaagt tggatttgtt
     9541 cctcttcaag tggtttttga gtcatggact gagctgcctt ttcgtagcgt ttttcttgtg
     9601 agaggacagt tttcatcttt ttagacaata ggcgaccggc agtactatct ttggtagctc
     9661 gaagcgcagt ttctacattt tgcttaactc tccgatgttt ttccatggtt ttatcgtagg
     9721 ctctttggtt gttagcagct tgctgacttt gtttggcaaa attagccttt ctctgctcac
     9781 tatagctatc atagtctaaa tgctctacta gcgtttccgc ttctttacgg tgtttgacca
     9841 gtcgcaagtg aacaatagtg tctgccgttt cagaaagaaa gtcttcatca tgggaaatga
     9901 aaataacggt ttgcctggtc ttttgaatct ggccttttag ccaatcaact gtctcaaggt
     9961 ctaggtcatt tgaaggttca tctaaaaata gaatctcaaa gggtttggct aactcatgga
    10021 taagctgaat tttcaaagct tctccccctg atagattgcc aatctcttgg tcacttgcga
    10081 aacgattgct atcaaaatgc aattcctccg ccaaacgata gaggatactg tagtctaaat
    10141 caatagaatc taaaaagaag tagtcgtgta aagttttctt tttcagctcc tcgggaagtt
    10201 tttgaggaat gtaggccagt gactgatagt cagattggat gtttcccttg atagtgaaat
    10261 cagacaaagc ttcccccatt aaaattttaa gtaaggttga tttgccattt ccttcttcac
    10321 caataatagc aaccttttcc ccgtcttgga tggtcatggt taggtcagat acaagatctc
    10381 gtaaatcttt gttttgtgtg atggttagat gattgatttt tatcatatga tttccctttc
    10441 tagaatgttc atagtgcata acaaaaagct ctactttacc tagtagagcc caaagtctat
    10501 gtcaaaaaaa ctctattaac atgccagaaa aagagcataa aagagcacac aaaaagagac
    10561 aaaaacaaga tctactaaat aaaggttctt tatttgatag acttagttgt tcatgtctcc
    10621 cttacctccg agtattagta tctttatatt atacctttaa ggaaattttg tcaacagaaa
    10681 actggaaatt ctatgctcta aaattcaaaa acaggtccat agagacttag tacgtgtttg
    10741 acatgctcgt aatggaattt atcatagttt cgccaagcag tgcgtgcttg attgtttagt
    10801 tttaggctac ccagtccaat cagaggactg gtgggttggt aaaatttctc aagatcaatt
    10861 aagatactgt tgttcaactt ttccgttttt ttgagctctt gcatagtagt gtttagcagc
    10921 tcttgtagtc gaatatcatc tgccgtaccc tgtttacagt tttggtagct tttatttaac
    10981 ttggatagga gttgaatgag tttcttgtct tccatgtgag catcctcctt gatctgatgt
    11041 actctttccc tatagtatag cactaaaaaa caaaactatc atggtaaata tgttaaaatt
    11101 tcgaatttta attacaaagt ttggtgtgct gatttgtagc cttttcatgg tataatcaag
    11161 agagtaaatc tttatggagg aagtatgaaa ttaaatattc aagaaattcg taagcagtct
    11221 gaaggtttga actttgaaca aacgttagat ttagttgatg acctgcgtgc acgtaatcaa
    11281 gaaattttag atgtaaaaga tatccttgca gttgggaaag tacaatatga agaccgtatg
    11341 tatttcttag attatcaact atcttatacc attgttcttg cttcgagtcg cagtatggag
    11401 ccagttgagt tagttgaatc ttatccagtc acggaagttt tcatggaagg cgcaactaac
    11461 cagctagatc aagaagtttt agatgatgac ttggtcttgc ccatcgaaaa tggggagctt
    11521 gaccttgctg agagtgtatc agacaatatc ctgctaaaca ttcctatcaa ggtcttgacg
    11581 gctgaagaag aagctggtca aggatttatc tcaggaaatg actggcaaat catgacagag
    11641 gaagaatacc aagctcaaaa agcagtaaag aaagaagaaa acagtccttt tgctggctta
    11701 caaggactat ttgacggaga tgaataatgg tcttgtcaaa aaaacgagca cgaaaggtgc
    11761 tagaagaaat cattgccctc ttcccagatg ccaagcctag tcttgatttt accaatcatt
    11821 ttgaactcct ggttgcggtc atgttatcag cccagacgac agatgcagcg gtaaataagg
    11881 ccacaccagg tctctttgtt gcctttccaa caccccaagc tatgtctgta gcgacagaga
    11941 gtgagattgc ttcacatatt tctcgcttgg gattgtatcg gaataaagct aaattcctta
    12001 aaaaatgtgc ccaacagtta ctagacgatt ttgatggtca agtccctcag acacgtgaag
    12061 aattggagag tttggcaggt gttggtcgca agacagccaa tgttgtcatg agtgtaggat
    12121 ttgggattcc agcctttgca gtggatactc atgtggagcg tatttgcaaa caccacgata
    12181 ttgtcaaaaa atcagcgacg ccacttgagg tggaaaagcg ggtcatggat atcttgccgc
    12241 ctgagcagtg gttagctgcc catcaggcca tgatttattt tggaagagcc atttgccatc
    12301 caaaaaatcc agagtgtgac cagtacccac aattatatga ttttagcaat ttgtaagatg
    12361 ctacattgaa actttttgct tgattttaag catgctttgt gctatagtaa atgataacta
    12421 aatattcctt taaacctgtc ccgtgaggca ggcaaggagc gcgataaagt agaagggtga
    12481 agtctatact gtgtgcagta gggctcgctt ggctataaat ttcaagtctc cttgtttaag
    12541 gagacttttc tttttggagg tttgtcatga aaacaaaaga agttgtagac gaattgaccg
    12601 tcaaacgagc gattacgcgt attacttatg agattatcga acgcaacaaa gatttgaata
    12661 agatcgtctt ggctggtatt aaaactcgtg gtgtctttat tgcccaccga atccaagaac
    12721 gtttgaagca gttagaaaat ctttcagttc ctgttgtgga attggatact aaacctttcc
    12781 gtgatgatgt taaaagtgga gaagatactt ctttggtttc tgtcgatgtg acagaccgcg
    12841 aagttatctt ggtggatgat gtgctctata caggtcgtac catccgtgct gctattgata
    12901 atattgtcgg tcatggtcgt cctgcgcgtg tgagtttagc agttctagtc gatcgtggac
    12961 atagagaatt gccaatccgt ccagattacg ttggaaaaaa tatcccaacc agtcgttctg
    13021 aagaaatcat cgtagagatg acagaacttg atgaccaaga cagagttctg attactgaag
    13081 aagcttagaa agttaaagga gtagccatgt cagaaaatca acaagcattg aaccatgtgg
    13141 tgtccatgga agacctcact gtcgatcaag taatgaaatt gatcaagcga ggaattgagt
    13201 ttaaaaatgg agcccagctt ccctatgaag accatccgat tgtttccaat ctcttctttg
    13261 aggattctac acggacacat aagtcctttg aagtcgcaga gattaaactt ggattggaac
    13321 gacttgactt tgatgtgaag actagttcgg ttaataaggg tgagacactt tatgacacca
    13381 ttttgactct gtctgcttta ggagtggatg tctgtgtgat tcgccatcca gaggtcgact
    13441 actatagaga attgattgct agtccaacga ttacgacttc catcatcaat ggtggagatg
    13501 gttcgggcca acaccctagc cagagcttgc ttgatttgat gaccatttat gaggaatttg
    13561 gccactttga gggtcttaaa gttgctattg caggtgactt ggaccactca cgcgttgcca
    13621 aatccaatat gcagattttg aaacgcttgg gagctgaact ctttttcgct ggacctgagg
    13681 aatggagaag tcaagagttt gcagactatg gaaagtttgt aaccattgat gaaatcattg
    13741 atcaggttga tgtcatgatg tttctccgtg tgcaacacga acgccatgat agtggagctg
    13801 tattttcaaa agaagactac catgcccaac atggcttgac tcaagaacgt tacgatcgtt
    13861 tgaaagaaac agcaatcctc atgcacccag ctccaatcaa tcgtgatgta gaaatagcag
    13921 accacttggt tgaagcacca aaatcacgga ttgtccaaca aatgaccaat ggtgtctttg
    13981 ttcgaatggc aatcttagaa tccgtactag cgagtagaaa cgccaactaa aacacaagac
    14041 gttaaaagac gccagtgagc gtccacgaga ggtgaaaata tgacaaaaag aattctagta
    14101 ttagaagatg gcacagtttt tgaaggcaag gccttcggag cagatattga tgtaacaggc
    14161 gaaatcgtct ttaatacagg gatgaccggc taccaagaat ccattacaga ccagtcttat
    14221 aatggacaaa tcttgacctt tacttatcct ttggtaggaa attatggtat taatcgtgat
    14281 gattatgaat ccattattcc aacttgtaag ggagtcgttg tttttgaaga agcgcgtaga
    14341 gctagcaact ggcgcaacca aatgaccttg gatgaatttt tgaaagccaa gaaaattcca
    14401 gggatttcag gaattgatac gcgtgctctt accaagatta tccgtaagca tggtactatg
    14461 cgtgcaacct tgacccatgt tggggacagt atggaccatg tgacggacca gctccaagca
    14521 acagttttac cgacagacaa tatcaaacag gtttctacta aaacgtctta tccagctcca
    14581 ggagttggtt tgagcgtggt gctagtggac tttggtctca agcactcaat cctacgtgaa
    14641 ctttctaaac gcaactgtaa cgtgacagtt gttccttatt cgacaacggc tgaagaaatt
    14701 ctccatctca atcctgatgg agttatgttg tcaaatggtc caggtaaccc agaagacgtt
    14761 ccacaagcac tcgacatgat tcgcggtgta caaggaaaaa ttccaatctt tggtatttgt
    14821 atgggacacc aactctttgc aatggcaaac ggggctaaga cctataagat gaagtttggc
    14881 caccgtggat tcaaccatgc ggtacgtgaa atcgcaacag gacgagtaga cttcaccagc
    14941 cagaaccatg gttatgcagt cagccgcgag gatttgccag agcacttgat tattacccac
    15001 gaagaaatca atgacaagtc agtggaaggt gtgcgccaca gataccaacc tgctttctct
    15061 gttcaatacc acccagatgc agctcctggg ccacacgacg ctagctacct atttgacgag
    15121 tttatcgaga tgatggaagt ttttaaacaa tcaaactaag aacgagtaaa agctcgtcgg
    15181 agaatttatg taactgaaca cggacggaaa gctcagaatg tagagaaacc aacttggatt
    15241 gtcaatcctt ccttgatttt ctaaaattca atcgctttct attcgtcctt tgtatcttat
    15301 ttaatgtcgc cctgagaggc gacgaataga aaggagaaga tacattgttc gcggaagtga
    15361 acacgcccta agaactgtgt gaaaaagata aacatcgtct tgacgctgaa ggcgtctgcg
    15421 ccgagtttcc tattttcact gtgttcttta cgggctttgt atcataaact atgcctaaac
    15481 gtactgatat tcaaaaaatt atggtgattg gttctggtcc gattattatt ggtcaggctg
    15541 ctgagtttga ctacgctggg acccaggctt gcttgtcgtt gaaagaggaa ggttatgagg
    15601 ttgttttggt taactcaaat cctgcaacca tcatgacgga caaggaaatt gctgataagg
    15661 tttacattga accgattaca cttgagtttg tgacacgtat tcttcgtaag gaacgtccag
    15721 atgccttgct accaacactc ggtggtcaga caggtctcaa catggccatg gaattgtcta
    15781 aaaatggtat cttagatgag cttggtgtag agcttctcgg tactagattg tctgccattg
    15841 accaagcgga ggaccgtgac ctctttaaac aattgatgga agagcttgag caacccattc
    15901 cagaatctga aattgttaac acagtagaag aagcaattgc ctttgcagcg acaattggct
    15961 acccagtcat cgttcgtcca gcctttaccc ttggtggtac tggtggtggt atgtgtgcca
    16021 acgagaaaga attgcgtgaa atcactgaaa atggtttgaa attgtcacct gttacccaat
    16081 gtttgattga gcgttcgatt gccggtttca aggaaattga atacgaagtg atgcgtgact
    16141 cagctgacaa tgccttggtt gtttgtaaca tggaaaactt tgacccagtt gggattcaca
    16201 caggggattc cattgtattt gcccctgcgc aaaccatgtc agactatgaa aaccaaatgc
    16261 ttcgtgatgc gagcttgagc attattcgtg ccctcaagat tgaaggtgga tgtaatgttc
    16321 agctagctct tgatcctaac agcttcaagt attatgttat cgaagtaaac cctcgtgtat
    16381 cacgttcgtc agcccttgct tctaaagcga caggatatcc gattgctaag ttggctgcca
    16441 agattgcagt aggtttgacc ttggatgagg ttataaaccc agttacaggt tcaacctatg
    16501 ccatgtttga accagccctt gactacgtag ttgctaagat tccacgtttc ccatttgaca
    16561 agtttgaaaa gggtgaacgc cgtctcggta cacaaatgaa ggcgactgga gaagtcatgg
    16621 caatcggtcg taacatcgag gaatctcttc tcaaggcctg ccgctccctt gaaattgggg
    16681 tgcaccacaa tgaaatacct gaacttgcag cagtttctga tgatgccttg attgaaaagg
    16741 ttgtcaaagc ccaagatgac cgtctcttct atgtgtcaga agccattcgc cgtggttaca
    16801 cacctgaaga aattgcagag cttacaaaaa ttgatatctt ctatcttgat aaactcttgc
    16861 atatctttga aattgagcaa gaattgggtg ctcatccaca agatctagaa gttttgaaaa
    16921 cagccaaact taatggtttt tcagaccgta agattgctga actctgggga acgactgatg
    16981 ataaagttcg ccaacttcgc ttggaaaaca agattgttcc agtttataag atggtagaca
    17041 cttgtgcggc agagttcgac tccgaaacgc catatttcta ttcaacctat ggttgggaaa
    17101 atgaatctat cagatctgat aaagaatctg tacttgtcct aggttcaggt ccaatccgta
    17161 tcggtcaagg ggttgagttt gactacgcaa ccgttcactc tgttaaggct atccaggcag
    17221 ctggttatga agctatcatc atgaactcaa acccagagac cgtttcaaca gacttctctg
    17281 tatcagataa gctttacttt gagccattga cattcgaaga tgttatgaac gtcattgact
    17341 tggaacaacc aaagggagtt atcgttcagt tcggtggtca aacagccatc aaccttgcgg
    17401 agccattggc aaaagcaggt gtgaccatcc ttggtacaca ggttgctgac ctagaccgtg
    17461 ccgaagaccg tgacctcttc gagcaagctc tcaaagaatt ggatattcca cagccaccag
    17521 gacaaacggc taccaatgaa gaagaagcag ctcttgcagc tcgtaagatt ggcttcccag
    17581 ttcttgtccg cccatcttat gtacttggtg gacgtgccat ggaaatcgtt gaaaacgaag
    17641 aagaccttcg ttcttacatg cgaactgctg ttaaggctag tccagaccac ccagttcttg
    17701 tcgactctta tatcgttggg caagagtgcg aagttgatgc catttcagat ggtaaaaatg
    17761 tactcattcc tggtatcatg gagcatatcg aacgtgctgg tgtccactca ggtgactcaa
    17821 tggctgttta cccaccacaa accttgtcgc aaaaggttca ggagaccatc gcagactaca
    17881 ccaaacgctt agcaatcggt cttcactgcc ttggaatgat gaacatccag tttgttatca
    17941 aggatgaaaa agtctacgtt attgaggtca atccacgtgc cagccgtacc gttccattcc
    18001 tatctaaggt aaccaatatt cctatggctc aagtagcgac caagctcatt cttggtcaaa
    18061 gtttatcaga acttggctac caaaatggac tttaccctga aagcacccgt gttcatatca
    18121 aggcacctgt cttctccttt accaaactag ctaaggtaga cagcttgctc ggtcctgaaa
    18181 tgaagtcaac aggtgaagtt atgggttctg atgctacttt ggaaaaagct ctctataaag
    18241 cctttgaagc ttcttatcta cacttgccaa cttttggtaa tgtcgtcttt accatcgctg
    18301 atgatgccaa agaagaagcc ttgaacttgg ctcgtcgttt ccaaaacatt ggttatggaa
    18361 tccttgcgac agaagggaca gcagccttct ttgctagtca tggcttgcaa gcccaacctg
    18421 ttggtaagat tggtgatgac gataaggata tcccaagttt tgtacgcaaa ggaagaattc
    18481 aagctatcat taacacagta ggaacaaaac gaactgctga cgaagatggt gagcaaattc
    18541 gtcgttcagc cattgaacac ggggtgcccc tcttcacagc gctagataca gctaatgcta
    18601 tgcttaaggt acttgaaagt cgtagctttg ttacagaagc gatttaaata gaaaaataag
    18661 atatagcaaa ctgaatttag aacag
//
LOCUS       DAVHEW010000034        18388 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVHEW010000034 DAVHEW010000000
VERSION     DAVHEW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.034"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            367..663
                     /locus_tag="VVL53_001069"
     CDS             367..663
                     /locus_tag="VVL53_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU4183256.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIYSNGASIG"
     gene            784..1728
                     /gene="manA"
                     /locus_tag="VVL53_001070"
     CDS             784..1728
                     /gene="manA"
                     /locus_tag="VVL53_001070"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU4183257.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1768..3115)
                     /locus_tag="VVL53_001071"
                     /pseudo
     CDS             complement(1768..3115)
                     /locus_tag="VVL53_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3343..4083
                     /locus_tag="VVL53_001072"
     CDS             3343..4083
                     /locus_tag="VVL53_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4183258.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAVYVQTNAE"
     gene            complement(4112..4567)
                     /locus_tag="VVL53_001073"
     CDS             complement(4112..4567)
                     /locus_tag="VVL53_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU4183259.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVNPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4587..5762)
                     /locus_tag="VVL53_001074"
     CDS             complement(4587..5762)
                     /locus_tag="VVL53_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4183260.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5821..6666)
                     /locus_tag="VVL53_001075"
     CDS             complement(5821..6666)
                     /locus_tag="VVL53_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU4183261.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6791..7348
                     /locus_tag="VVL53_001076"
     CDS             6791..7348
                     /locus_tag="VVL53_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4183262.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7367..7834
                     /locus_tag="VVL53_001077"
     CDS             7367..7834
                     /locus_tag="VVL53_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU4183263.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7923..8552
                     /gene="upp"
                     /locus_tag="VVL53_001078"
     CDS             7923..8552
                     /gene="upp"
                     /locus_tag="VVL53_001078"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU4183264.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLNLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8726..9316
                     /gene="clpP"
                     /locus_tag="VVL53_001079"
     CDS             8726..9316
                     /gene="clpP"
                     /locus_tag="VVL53_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU4183265.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9395..9643
                     /locus_tag="VVL53_001080"
     CDS             9395..9643
                     /locus_tag="VVL53_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU4183266.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9744..10904
                     /locus_tag="VVL53_001081"
     CDS             9744..10904
                     /locus_tag="VVL53_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4183267.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11172..12041
                     /locus_tag="VVL53_001082"
     CDS             11172..12041
                     /locus_tag="VVL53_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU4183268.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12045..13001
                     /locus_tag="VVL53_001083"
     CDS             12045..13001
                     /locus_tag="VVL53_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU4183269.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13001..13765
                     /locus_tag="VVL53_001084"
     CDS             13001..13765
                     /locus_tag="VVL53_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183270.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13765..14475
                     /locus_tag="VVL53_001085"
     CDS             13765..14475
                     /locus_tag="VVL53_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183271.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGRIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14783..15439
                     /locus_tag="VVL53_001086"
     CDS             14783..15439
                     /locus_tag="VVL53_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4183272.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15547..16642
                     /gene="prfB"
                     /locus_tag="VVL53_001087"
     CDS             join(15547..15618,15620..16642)
                     /gene="prfB"
                     /locus_tag="VVL53_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU4183273.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16660..17352
                     /gene="ftsE"
                     /locus_tag="VVL53_001088"
     CDS             16660..17352
                     /gene="ftsE"
                     /locus_tag="VVL53_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU4183274.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17345..18271
                     /locus_tag="VVL53_001089"
     CDS             17345..18271
                     /locus_tag="VVL53_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183275.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5647 a         3462 c         3986 g         5293 t
ORIGIN      
        1 ttctataaat taatttgact ttcctgatag agttgttcac atcttatttc aaactactat
       61 aaaagttcta taatctcttt ataagatttg cccatcagac gaaatatagt agattgaaac
      121 tagaatagta cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatcg
      181 atttgtactg ttcttatttc attttactat agaacgattt gaaggcgttt atgatattta
      241 gctgtacgag agtcttttaa aagtgttttg atggtttgga tttcttcttt agttgatttc
      301 atatcactat tatataatgc tttttgattt tagtttagta ttatcgtaga aaatggactc
      361 ggtgctatgg atacaccgga tgagaatggt tatgtagcag atgactatcg gattacttac
      421 ttagaggccc acatcaaggc catgcgagat gccatttacc aagacggggt tgacttgctt
      481 ggttatacga cttggggctg tatcgatccg gtttcagctg gcacagggga aatgaataag
      541 cgctatggct ttatctatgt agatcgagat aatgtaggaa acggagctct caaacgtagc
      601 aagaagaagt ccttctattg gtacaaggat gtcatttata gcaatggtgc aagcatcggg
      661 tagagcacat ttgtttacta tttttataaa atcttctccc tgctttataa gatgtaaaaa
      721 agagttcaat tcgaactctt tttttgctat aatgagggga gaaaaatcag acaggagatc
      781 gacatgtcag aaccattatt tttacaatca gttatgcaag aaaaaatctg gggtggagcc
      841 aagctacgtg atgagtttgg ctacgacatc ccaagtgaaa aaatcggaga atattgggcc
      901 atctcagccc atccaaatgg agtctctaaa gttgccaatg gtcgttacga gggaacagat
      961 cttgctactt tgtatgcgga acaccgtgaa ttatttggca atcgtccaga acctgtattt
     1021 ccacttttga ccaagatcct cgatgccaac gactggctca gtgtccaagt tcacccagac
     1081 gatgcttatg gactcgagca tgaaggcgaa ctcggaaaaa cagaatgctg gtacattatc
     1141 gcagcggatg aaggttcaga gattatctac ggtcacaatg ccaagtcaaa agaagaactc
     1201 cgccagcaaa tcgaggacaa gaactgggat gacttgttga ccaaagtccc tgttaaggct
     1261 ggagatttct tctatgtacc aagcgggact atgcacgcta tcggggcggg tatcttaatc
     1321 cttgaaaccc agcagtctag cgataccacc tatcgcgtct atgactttga ccgcaaggat
     1381 gacaaaggca acttgcgtga acttcatctt gaaaaatcca tcgatgtctt gaacattggt
     1441 gagccagcaa atagccgtcc tgtaactgtt aaagcagatg atttgcgttc cactctcctt
     1501 gtatctaatg atttcttcgc agtttacaag tgggaaatta ctggaaaagt tgactttgaa
     1561 aagacagctg actacagctt attgagtgtc ttggctggtc aaggtcagct aactgttgac
     1621 gggaaaaatt atccaattca aaaaggcagc cactttatcc taccaagtga tgttgaagct
     1681 tggactctgg aagggcaagg tttggaattg attgttagcc atccataaaa agaaagggcc
     1741 tgagttcact actcaagtcc tttttgatta cataaattgg gcaatgaaga ccacaatgat
     1801 gatgattgga atgacgaaac gaagaaacga agaaggaaga gccagacttg gaacagtcct
     1861 tgtttccatg ctctttcatc gagatggagt tcctccattg caagagcctt tttaaagata
     1921 tagcctgtaa aaagtgaaag gtagagagct ccaaatggca tgaggagatt ggaaaccaag
     1981 aagtccatag cgtcaaagaa ggtcttacca aaaatgtgaa catccgccat gacaccgtaa
     2041 gatagggctg aaggaatgcc aaagacaaag gtcaaaattc ctaaaataac actccatttg
     2101 gcacgtttgc tgttatcctg gttggtgata ttgtctacat tgatttccag catcacgaca
     2161 gaaaaagtga ctgtcgcaaa aaggaagagc aagaggaaga ggacgtagaa aatggttcca
     2221 aaaggcatct tgtcaaagag ttgaggcaag acgataaaga gcaggctggg tcccccttca
     2281 gactggatat tgaaggggga tcgagcttgg aaaatggcta gacctgccat gatggatatc
     2341 gagatattca tggctacgat ggagattcct gactggacta gattggtttt cttgtctaag
     2401 taagaagcat aggtcaacat gactgtaacc cctagtgaga gggcaaagaa agattgtccc
     2461 agagcataga ggagaccagt gctagtcagt tttgaaaagt ctggtttgag gaagtaaaga
     2521 accccttcca tggcatttgg caaactgaga gagcgaccga tgataaaaac aaagacgata
     2581 aagagcaggg gcatcatgac tttcgaagct ctttcaatcc ctttttgaac cccacgtgat
     2641 acaatgaaga tattcaatag gataaaggcc gcttgagctc ctagggcaat ggctggattt
     2701 gaaatgattg aagtaaataa ctgagcataa tcacccgttc caccaagttg gaacaatttc
     2761 ccaaactcaa tacctagata gactagaatc catcctccga taacactgta aaaagataag
     2821 aggataaaga gggcaaaggc gccaatccaa ccgataaagt tgtacttgtt attcttgccc
     2881 agttttccaa aggttttgat agcggaaacg ccagcactac ggccaagggc aaactcagcc
     2941 agcaggagag ggaaaccgat taaaatagtg gaaatgagaa agattagtaa aaagcctcca
     3001 ccgccattag cagcagtcat gtagggaaac ttccaaacgg aaccaagccc gatggccagc
     3061 cagcagatgc tagaataaaa ccaagtttcg acccccattg tgatttttct gacatagtta
     3121 aactctgatc tcctttttca aaataagaaa aagctacttg agttgtcaaa tagccttctg
     3181 tttgattgat agacttgact atcctatcat gttttctaag gtctgtcaag aaaatcattt
     3241 tcaagttttc acacctttct caaaaaagtt aaaaaatttt ctcaaaaacg cttgactctg
     3301 acctaaggcg aggggttata ctatcattgt aaggaggaaa tcatgtacca tataaaagaa
     3361 gctgcgcagc tttcaggtgt ctctgtcaag accctgcatc actatgacaa gataggactc
     3421 ttggtcccct taaagtcgga aaacggctat cgaacctaca gtcaagagga tttggaacgc
     3481 cttcaggtca ttctttacta caaatatcta ggcttttctt tagagaaaat agcagagctg
     3541 ttaaaggaag aaaggacaga tttattgccc catttgacta ggcagttgga ctatctaact
     3601 cgcgaaaggc aacatctgga taccttgatt tccaccttgc aaaaaactat tcaagaacaa
     3661 aaaggagaaa gaaaaatgac cattgaggaa aaattcacgg gatttagcta tcaagacaat
     3721 caaaaatacc accaagaagc ggtagagaaa tatggtcaag aagtcatggg acaagcgctc
     3781 gaacgccaaa aaggtcacga agacgaggct acggccgcct ttaaccaagt ctttcaaact
     3841 ttggcacaaa atcttcaagt tggtttacct gcaacagcaa ccgaaaacca ggagcaagca
     3901 gccaagctct tgcaagccat tcgcacttat ggatttgact gctctattga ggtattcggt
     3961 catatcggta aaggttacgt ctacaaccca gagtttaagg aaaacattga caagtttggt
     4021 tctgaaacag cccagtacac gtcagatgcc attgcggttt acgttcagac aaatgcagaa
     4081 taaataggct aggaatttcc tagcctattt tttacttcaa atcataaagc cagtcgtcac
     4141 cgtttttgta gtaaaagaat tcactgagat cttcttctag aaacacacga agcatatcag
     4201 acatatcatc ggttgcaagt tttagatgag aaagattttc aaagtcctcc caccaaactt
     4261 tcccttcgtc tgaagactgg agttcaccag taaagtgttc tgtcttgtaa aaaaggacga
     4321 cataacgata atccttgtcg tcataccagt ttttgatacc acagagttgg ggtttggaaa
     4381 tgatcagacc agtttcttct ttcacttcac gaatgacagc atcgacaaag gattcgccac
     4441 gttcaacatg accaccagga aaagtaatgc cagaccagtc gggattaact cggtcttgga
     4501 ccaggacctt atctccgttt ttaatcatac acatgttaac aaattcgact gcctctcttc
     4561 tgttcattct tcacaacctt taatctttaa tcataatgca gacttcccgc cacccagccg
     4621 gtacagaggg cagaagtgat gttaaagcca cccgtgtggg cattgatatc cataacttcg
     4681 cctgcaaagt ggaggccagg taccagctta ctttcaaggg ttttaggatt gatttccttg
     4741 agactgactc cacccttggt aacaaaggac tttgcaaggg acatttttcc agttacagga
     4801 attttaagtt ctttaatgga ctggacaagt tgttctcgtt ccttttcagt cagttgtttg
     4861 actttttcag gatatccttg tacaaaaaat tcggccaagc gttctggtaa caaggttttt
     4921 aaagcgtttt tcaaggattt ttcccgattt tcttctagaa atgtaaccaa gtccttctca
     4981 gaaagttgag gcaaaacatc gagtgagaga acctccccac ctttgacaaa gctagacatg
     5041 cgtagggcag caggacctga caaaccaaag tgggtaaaga gtaaatcatg agtgatgaca
     5101 tgcttaccat aacttagggt cacatcgtcc agagaaatac cttgtaaggc tttatgtgga
     5161 aaatctgtta ataaaggact ttcagcagcc tcaagatcgg tgatggtatg cttaaaatgg
     5221 cgagcaatct cgtgaccaaa accagtcgaa ccagtcgaag gataagactt accacctgtt
     5281 gtgacaatga gtttctcaca agtgaaggtt tgatccgctg acttaaggac aaactggtca
     5341 tctacttttt taacagaaac gatttctatt tgagtagcaa cttgaccacc tagttcggtg
     5401 attttctttt ccaaagcttc gataatagtc cgagacttgt cactggctgg aaagacgcgt
     5461 ccgtggtctt cgaccttaag tttaacacca ttttctgtaa aaaagttgat gatgtcatga
     5521 ttatcgaact gggagaaaac actgtaaaga aagcgtccgt ttccaggaat tccagctagc
     5581 aggttgtcta agctaccatt gttggtcaca ttgcaacgtc ccccaccagt cccagctaat
     5641 ttttttccaa gtttccgatt tttttcgatg aggagggttt tctgtccata aaagctactg
     5701 gaaatcgtag ccatcatacc agcaggtccc ccaccgatga caatagtatc aaaatgtttc
     5761 atagctctat tgtaccacaa aaaaacaaga gatgatggtc acctcttgtc aagaatgcaa
     5821 ttaatcaatt tcatagccca tcagcaaacc gccctcttct gcatagaaac tgcagagacc
     5881 agaggttggt agaattttaa tatccgcttg tgggaaggtt tcacggattc gctctgagag
     5941 ctgttgacaa catttttcgt tattgcgttg ggccatgaca atacggccac cagcatatcc
     6001 agcttttact aactcatcat aggcagcttg aactgatttc tttgatcccc ttgctttttg
     6061 tagcaattcg agagtcccag tttcactagc ttttccgacc atacgaatgt tgagaaggcc
     6121 aacgaccgta ccgataagct tgctcaaacg gccgttcttc accaagttat cgactttggc
     6181 taggacaaag agcaacttag ttttttcttg ataggcggtg atagcttcaa ccacttcttc
     6241 aaaagacaag ccctggtcaa tcaagtcatt caatttttct acgagtaggt caacttcacc
     6301 accagcagat aaactatcaa tcacatgaat cttagtgtca ggatggtctt ccagataaat
     6361 attctttgct agttgagcac tattgtgact gccagaaagg gtacctgtga tggttactag
     6421 gaaaatgttt ttggcacctt caaatgctcg caaatagtca tctgggcttg gacaagccga
     6481 ttttgaagct tctgcagttg catacatggt ttccatcatt tggtcaatat cgagactggc
     6541 gtcatcaaca aagacctgat cagctacttg aatggttaag gggacactta caaaggttgt
     6601 gttaatagct ggtgttggca gttgacgata atcacaacca gagtcagcaa taatcttcca
     6661 agtcatagaa attctccatc tttgtcagtt atacattgac aaaggttctg tcttttttta
     6721 caattatatc atgaaaaccc ttgaaacaaa agcatcatcc gtctgttgtg acaagtagaa
     6781 agaaaatgtt atgtctgaac gtagaatctc tgaaaagtct cttgaaaatc tcagaaaatc
     6841 aaaccaagaa tccaatttat taaccagaga agccattgaa acagccctct tgcaactctt
     6901 ggaaaaaaag gaactgacca agattagtat ttctgaattg gtcaaacgtg caggtgtttc
     6961 gcgtgcggcc ttttatcgca attatgactc caaagaggaa attttagaga gcgtctttaa
     7021 acgaactgtc cacaatatta tggaacagct gcatcattac gatttaaaga cagaccttta
     7081 tttggtttgg gttcaccttt tccgggaggc cagaaaggaa gctagagtaa ttcaattggc
     7141 cttggattac catctggaaa aaatctttgt ccaagccatg caggaatttc tagaaaaata
     7201 ccatgggaaa tcaaaaggtg tcagctctta tcttcattcc ttctggagct cagccatcgt
     7261 ctctgtcctt ctaaaatgga tcaaggatgg catgaaggta ccagctgaaa agattgcaga
     7321 tttaggttta ccatttttta aaaaatagag aaaaaggaga aaagagatga ctgaaaaaag
     7381 actggcctgg gatgagtatt ttgcagccca agctctacta attgcgaatc gttccacttg
     7441 taaacgtgct aaagtgggcg cgattctggt aaaagataat aaggttattt ccactggtta
     7501 caatggttcg gtgtcaggga ctgagcattg tattgatcac gaatgtttgg taattgaagg
     7561 ccactgcgtt cgcacccttc acgctgaggt caatgctatc cttcaaggtg ctgagcgtgg
     7621 tgttcccaaa ggctttacag cctatgtaac ccattttcct tgtctcaact gtacaaaaca
     7681 attgcttcag gtcggctgca agcgcgtggt ttatatcaac cagtaccgaa tggacgacta
     7741 cgcccaatac ctttatcaag aaaaagggac agaattgacc catttaccac ttgagacagt
     7801 acagacagct cttaaagagg ctgatctaat gtaaaaatta tcaaaaataa atggtttaga
     7861 aagattttta aaccgttttt tggtataata agaagaataa attgaaagaa ggaattccaa
     7921 aaatgggaaa aattgaagtt attaatcacc cactgattca acacaaattg tcaatcttgc
     7981 gtcgtacaga tacttctaca aaagcttttc gtgagctagt agatgagatt gcaatgttga
     8041 tggggtatga agtacttcgt gatcttccac tagaagatgt ggaaatcgaa acaccaatta
     8101 caaaaacagt tcaaaaacaa ttggcaggta agaaattggc catcgtccca atcttgcgtg
     8161 caggtatcgg gatggttgat ggtctcttga acttggttcc agctgctaaa gttggccaca
     8221 tcggtatgta ccgtgatgaa gaaacacttc aaccagttga atacttggtg aaattgcctg
     8281 aggacattga ccaacgtcaa atttttgtag tagacccaat gttggcaaca ggtggctcag
     8341 caatcttggc tgttgattct cttaaaaaac gtggcgcatc aaatatcaaa tttgtctgcc
     8401 ttgtatctgc tccagagggt gtaaaagccc ttcaagaagc tcatccagat gtagaaatct
     8461 ttacagcagc cttggatgaa cgtttgaacg aacacggtta tatcgttcca ggtcttggag
     8521 atgctggaga ccgcttgttc ggtacaaaat aagatcgaaa agaagaatga cttggaaaag
     8581 tatttccagt cacgaaagga ggttgggttt ttgtttctgt ctaatgaaag cagagcaaaa
     8641 atttgacctt ttttgaccaa gatattataa tagtcttatc ttcagtcatt tgaccaacaa
     8701 aattaaaact caaaaggaga aatgaatgat tcctgtagtt attgaacaaa caagccgtgg
     8761 agaacgttct tacgatattt actcacgtct tctcaaagac cgcatcatta tgctgacagg
     8821 tccggttgaa gacaatatgg ctaactctgt tattgcccaa ttgcttttct tggatgccca
     8881 agatagtaca aaagatattt acctttatgt caatacacca ggtggttctg tttcagctgg
     8941 tttggcaatc gtagatacca tgaactttat caaggcagat gtccaaacca ttgttatggg
     9001 aatggctgca tctatgggga ctgtcatcgc atcaagtgga gcaaaaggca aacgtttcat
     9061 gcttccaaat gctgaataca tgattcacca accaatgggt ggtacaggtg gtggtaccca
     9121 acaaactgat atggctatcg ctgcagaaca cttgctcaaa actcgtaata ccttggaaaa
     9181 aatcttggct gaaaattcag gtcagtcaat ggaaaaagtc catgcagatg cagaacgtga
     9241 taactggatg agcgcccagg aaacacttga atatggcttt attgatgaaa ttatggccaa
     9301 caattcattg aactaatgat gatagaaggc aaactcgact gggtttgctt tttttggtat
     9361 aatagggaga gatttcttag aaagaggatt tatcatgttt gaaaaagtca atcgctctgg
     9421 cttgattatc tatctttact ataatcgtga tgccaaaaaa ctgcaggatt atggagatat
     9481 tacctatcat tccaagaaac atcgttactt acaactctat gttccaactc aagaagtgga
     9541 gcaattggtc ggacgcttga gcaaggaaaa atttattaaa aaagttaggg tttgtcatat
     9601 ccaagagttg gaaacaccct ttgtgggcaa tctttatcga taggaaaacg ttatcatcga
     9661 aaaagttcaa ggaaaacatt gacaattttc tgataattcg gtatattctt aacagactat
     9721 ttaagaaata aggagacaaa aagatgaaga aaaaatttgc cctatcgttt gtggcgcttg
     9781 caagtgtagc acttcttgca gcctgtggag aagtgaagtc tggagcagtc aacactgctg
     9841 gtaactcagt agaggaaaag acaattaaaa tcgggtttaa ctttgaagaa tcaggttctt
     9901 tagctgcata cggaacagct gaacaaaaag gtgcccaatt ggctgttgat gaaatcaatg
     9961 ccgcaggtgg tatcgatgga aaacaaatcg aagtagtcga taaagataat aagtctgaaa
    10021 cagctgaggc tgcttcagtt acaactaacc ttgtaaccca atctaaagta tcagcagtcg
    10081 taggacctgc gacatctggt gcgactgcag ctgcggtagc gaacgctaca aaagcaggtg
    10141 ttccattgat ctcaccaagt gcgactcaag atggattgac taaaggtcaa gattacctct
    10201 ttattggaac tttccaagat agcttccaag gaaaaattat ctcaaactat gtttctgaaa
    10261 aattaaatgc taagaaagtt gttctttaca ctgacaatgc cagtgactat gctaaaggga
    10321 ttgcaaaatc tttccgcgag tcatacaagg gtgaaatcgt tgcagatgaa actttcgtag
    10381 caggtgacac agacttccaa gcagccctta caaaaatgaa agggaaagac tttgatgcta
    10441 tcgttgttcc tggttactat aatgaggctg gtaaaattgt aaaccaagcg cgtggcatgg
    10501 gaattgacaa accaatcgtt ggtggtgatg gattcaacgg tgaggagttt gtacaacaag
    10561 caactgctga aaaagcatca aacatctact ttatctcagg cttctcaact actgtagaag
    10621 tttcagctaa agctaaagcc ttccttgacg cttaccgtgc taagtacaat gaagagcctt
    10681 caacatttgc agccttggct tatgattcag ttcaccttgt agcaaacgca gcaaaaggtg
    10741 ctaaaaattc aggtgaaatc aagaataacc ttgctaaaac aaaagatttt gaaggtgtaa
    10801 ctggtcaaac aagcttcgat gcagaccaca acacagtcaa aactgcttac atgatgacca
    10861 tgaacaatgg taaagttgaa gcagcagaag ttgtaaaacc ataatagaaa aatgttgaaa
    10921 tagggaatga gcctttgact cactccctgt ttcgatattt aatactcttc gaaaatctct
    10981 tcaaactgcg tcaacgtcgc cttggattat atatgtgact gacttcgtca gtcttatcta
    11041 caacctcaaa gcagtgcttt gagcaacctg cggttagttt cctagtttgc tctttgattt
    11101 tcattgagta taagaaccta tcaaaaagtg agggaaaacc ctcggaatta taaatagaaa
    11161 gagtgaatct tatgctccaa caactcgtaa atggtttgat tctaggtagt gtttacgcgc
    11221 tgttagccct aggatatacc atggtttacg gaattatcaa gctcatcaac ttcgcccatg
    11281 gtgatattta tatgatggga gcctttatcg gttatttctt gatcaattct ttccaaatga
    11341 atttctttgt agcgcttatt gtagctatgc tagcgacagc tattcttggt gtcgtgattg
    11401 agtttcttgc ttaccgacct ttgcgccact ctactcgtat tgctgttttg attacggcta
    11461 ttggggtttc tttcctattg gagtatggaa tggtctatct ggttggtgcc aatacccgtg
    11521 ccttccctca agcgattcaa acagttcgat atgatttggg accaattagc ttaacaaatg
    11581 tgcagttaat gattttggcc atttccttga ttttgatgat tttgttacaa gtcattgtcc
    11641 aaaagactaa gatggggaaa gccatgcgtg cagtatcagt agatagcgac gcggcgcaat
    11701 tgatggggat caatgtaaac cgtacgatta gctttacctt cgctttgggt tctgctcttg
    11761 cgggtgcggc tggtgttctg attgctcttt attataactc tcttgagcct ttgatggggg
    11821 ttactccagg tcttaaatct ttcgttgccg cagtacttgg tggtatcgga attattcctg
    11881 gtgcggctct tggtggcttt gtgattggtc tattggaaac ctttgcgact gcctttggga
    11941 tgtcagattt ccgtgatgcc attgtttatg gaatcttgtt gttgatcttg attgtccgcc
    12001 cagctggtat ccttggtaag aatgtgaaag agaaggtgta aacgatgaag gaaaatttaa
    12061 aagttaatat tctatggtta ctccttttgt tagctggcta tagcttgatt agtgtactgg
    12121 tttcagtcgg agtacttaat ctattctatg tacagatttt acaacaaatt ggaattaata
    12181 ttattttggc tgttggtctc aacttaatcg ttggtttttc aggacaattt tcacttggtc
    12241 atgctggttt catggcgatt ggtgcctatg cagcagctat tattggttct aaatcaccaa
    12301 cctacggtgc cttctttgga gctatgcttg taggggcttt gctttcagga gcagttgcct
    12361 tacttgtcgg cattccaacc ttgcgcttga agggggacta tcttgcggta gcaactctgg
    12421 gtgtttctga aattatccgt atctttatca tcaatggtgg aagccttaca aatggtgcgg
    12481 caggtatctt agggattcct aactttacaa cttggcaaat ggtttacttc tttgtcgtga
    12541 ttacaaccat tgcaaccttg aacttcttgc gtagcccaat tggtcgttca accctctctg
    12601 ttcgtgaaga tgaaatcgct gctgagtcag ttggggttaa tacgactaaa attaaaatca
    12661 tcgcttttgt ctttggtgcc attactgcaa gtattgctgg gtcacttcag gcaggattta
    12721 tcgggtctgt tgtaccgaaa gattacacct tcatcaactc aatcaacgtt ttgattattg
    12781 ttgtatttgg tggactcggt tccattacag gtgcgattgt ttcggctatt gttctgggaa
    12841 ttttgaatat gcttctccaa gatgttgcta gtgtgcgtat gattatttac gctttggcct
    12901 tggtattggt aatgattttc agaccaggtg gactccttgg aacatgggaa ctgagcctat
    12961 cacgtttctt taaaaaatct aagaaggagg aacaaaacta atggcattac ttgaagtaaa
    13021 acagttaacc aaacattttg gtggtctaac agctgttgga gatgtgactc ttgaattgaa
    13081 cgaaggggaa ctggttggat taatcggtcc aaacggagct gggaaaacca cccttttcaa
    13141 ccttttgacc ggtgtttatg aaccaagcga gggaacagta accctagatg gtcacctttt
    13201 gaatgggaaa tcaccttata agattgcctc tttgggactt ggacgtactt tccaaaatat
    13261 ccgtctcttt aaagatttaa cagttttaga taatgttttg attgcttttg gaaaccatca
    13321 caaacagcat gtttttacta gtttcttacg cttaccagct ttttacaaga gtgaaaaaga
    13381 attaaaggct aaagctttgg aattgttgaa aatctttgat ttagatggtg atgcagagac
    13441 tcttgctaaa aatctttcct acggacaaca acgtcgtttg gaaattgttc gtgcccttgc
    13501 tacggaacct aaaattctct tcttagatga accagcagca ggtatgaacc cacaggaaac
    13561 agccgaattg actgagttaa ttcgtcgtat caaagatgag tttaagatta caatcatgtt
    13621 gattgaacac gatatgaatc tggtcatgga agtaacagaa cgtatctacg tacttgaata
    13681 tggccgttta atcgctcaag gaactccaga cgaaattaag accaataaac gcgttatcga
    13741 agcttatcta ggaggtgaag cctaatgtct atgttaaaag ttgaaaatct ttctgtgcat
    13801 tacggtatga tccaagcagt tcgtgatgta agctttgaag ttaatgaagg agaagttgtt
    13861 tcccttatcg gtgccaacgg tgcaggtaag acaactattc ttcgcacctt gtcaggtttg
    13921 gttcgaccaa gttcaggaaa gattgaattt ttaggtcaag aaatccaaaa aatgccagct
    13981 cagaaaatcg tggcaagtgg tctttcacaa gttccagaag gacgccacgt ctttcctggc
    14041 ttgactgtta tggaaaatct tgaaatggga gctttcttaa agaaaaatcg tgaagaaaat
    14101 caagctaact tgaagaaggt tttctcacgc tttcctcgtc ttgaagaacg gaagaaccaa
    14161 gatgcagcca ctctttcagg gggggaacaa caaatgcttg ccatgggacg cgccctcatg
    14221 tcaacaccaa aacttcttct tttagatgaa ccatcaatgg gacttgcccc aatctttatc
    14281 caagaaattt ttgatatcat tcaagatatt cagaagcaag gaacaacggt cctcttgatt
    14341 gaacaaaatg ccaataaagc acttgcaatc tctgaccgag gatatgtact ggaaacaggg
    14401 agaatcgtcc tatcaggaac aggaaaagaa ctcgcttcat cagaagaagt cagaaaagca
    14461 tatctaggtg gctaaaacaa tccagtggat tgttttagtc ggcagatgga gattacgaag
    14521 taatcatcaa tatagtccgg gggacctttt tagtcggtag attgagattg caaacaaatc
    14581 tgcatctaca ttgaaagctt aatttctaat aattgaaaaa atcgaatgaa aaatttctta
    14641 ccttcattca cagagctcga tttcagagct ctttttgcta gcttattcat acttttctga
    14701 atttcgaaaa agaaatgtaa gcgtttgata gatttacaaa aagattgtat aatagggata
    14761 agaatagaaa aggagaagtc tcatggcagt taaagatttt atgacccgca aggtagttta
    14821 tattagtcca gatacaacag tatctcatgc agcagatttg atgagagagc aaggtttgca
    14881 ccgtctgcct gttatcgaaa atgatcaatt agttggtttg gtgactgagg gaaccattgc
    14941 acaagcaagt ccatctaaag caacaagtct ttctatctat gagatgaatt atcttctgaa
    15001 taagacaaaa gtaaaagatg tcatgattcg cgatgttgtc actgtctcag gctatgctag
    15061 tctagaagat gcaacttatc tgatgttgaa aaataagatt agtattctcc ctgtcgtaga
    15121 taaccatcaa gtatacggag ttattactga ccgtgacgtt ttccaagcct ttcttgaaat
    15181 tgcaggttat ggcgaagaag ggattcgtgt acgctttgtt acagaagatg aagttggtgt
    15241 tcttggaaaa attgtttctt tgattgtaga agaaaatttg aatatctccc atacagtcaa
    15301 tattccgcgt aaggatggta aggtgattat cgaagtgcaa atcgatggat caattgattt
    15361 accagccttg aaagaaaaat ttgaagcaaa tggtattcaa gtggaagaaa tcgctcgcac
    15421 ttcagcaaaa gtcttgtaag aagggaagcc caaaggcttc ttttttcatg aaaaggggat
    15481 tagagcaaaa gatggaaaga aatgataaaa tatgctataa tgaaataatg taaaaaagga
    15541 gtatttatgg acatttcagt aattcgtcag aaaattgacg caaatcgtga aaaattagct
    15601 tctttcaggg ggtctctttg acctcgaagg gctagaggaa gagattgcca tcttggaaaa
    15661 caagatgaca gaacctgatt tttggaacga taatattgcg gcccaaaaaa cgtcgcaaga
    15721 attaaatgaa ttaaaaaaca cttacaatac cttccataag atggaagagt tgcaggatga
    15781 agtcgaaatt ttattggatt ttttggctga agacgagtca gtgcatgatg aactggtagc
    15841 gcagttagcc gaacttgata agataatgac cagctacgag atgactctac tcttgtcaga
    15901 accttatgac cacaacaatg ccatcttgga aatccatcca ggttctggtg gtactgaggc
    15961 gcaggactgg ggtgatatgt tgcttcgtat gtatactcgt tatggtaatg ctaaaggctt
    16021 taaagtggaa gtgttggatt accaagcagg tgatgaggct ggtattaagt cggtaacttt
    16081 atcatttgaa gggcctaatg cctatggtct cctcaagtca gaaatgggtg ttcaccgctt
    16141 agtgcgaatc tcaccatttg actctgccaa acgtcgccat acctctttca catctgtaga
    16201 agtgatgcca gaattggatg atactattga agtggaaatc cgtgaagatg atatcaagat
    16261 ggataccttc cgttcaggtg gtgccggtgg acaaaacgtc aataaggttt caacaggtgt
    16321 acgtttaacc cacattccaa ctggaattgt tgtccaatca acagtagatc gtacccagta
    16381 tggaaataga gatcgtgcca tgaagatgtt gcaggctaag ctctatcaaa tggagcaaga
    16441 taagaaggct gcggaggtag attctctcaa aggtgagaaa aaggagatca cttggggaag
    16501 ccaaatccgt tcttatgtct tcacgcctta tactatggta aaagatcacc gaactagctt
    16561 tgaggttgct caggtagata aggttatgga tggggaccta gatggtttta tcgatgctta
    16621 tctcaagtgg cgaattagct aagatagaaa ggaactcaca tgtcaattat tgaaatgaga
    16681 gatgtcgtta aaaaatacga caacggaaca actgctctac gcggtgtttc ggttagcgtt
    16741 caaccggggg aatttgctta catcgtagga ccttcaggag cagggaagtc aacttttatt
    16801 cgttctctgt atcgtgaagt aaaaatcgat aaaggaagcc tatcagttgc tggttttaat
    16861 ctggttaaga tcaaaaagaa agatgtcccg cttctacgtc gtagtgttgg ggttgtcttc
    16921 caggattata aattgttacc aaagaaaact gtctatgaaa atattgctta cgctatggaa
    16981 gtaatcgggg aaaatcgccg taatatcaaa agacgagtga tggaagtttt ggacttggtt
    17041 ggattgaagc ataaggttcg ttctttccca aatgaactct caggtgggga gcaacagcgg
    17101 attgcgattg cgcgtgcaat tgtaaataat cccaaagtat tgatagctga tgagccaaca
    17161 ggaaatctgg atccggataa ttcatgggaa attatgaatc tcttggaacg gattaaccta
    17221 caaggaacaa ctattttgat ggcgactcat aatagccaga ttgtaaatac cttgcgccac
    17281 cgtgtcattg ccattgaaaa tggccgtgtc gttcgtgacg aatcaaaagg agagtatgga
    17341 tacgatgatt agtagatttt ttcgccattt atttgaagcc ttaaaaagtt tgaaacgaaa
    17401 tggttggatg acagtagctg ctgtcagttc agtcatgatt actttgacct tggtggcaat
    17461 atttgcatct gttattttca atacagcgaa actagctaca gatattgaaa ataatgtccg
    17521 tgtagtagtt tatatccgaa aggatgtgga agataatagt cagacaattg aaaaagaagg
    17581 tcaaactgtt acaaataatg actaccacaa ggtatatgat tctttgaaga acatgtctac
    17641 ggttaaaagt gttacctttt caagtaaaga agaacaatat gaaaaattaa ccgagataat
    17701 gggagataac tggaaaatct ttgaaggaga tgccaatcct ctctatgatg cctatattgt
    17761 agaggcaaac actccaaatg atgtaaaaac tatagccgaa gatgctaaaa aaattgaagg
    17821 tgtctctgag gttcaagatg gcggtgccaa tacagaaaga ctcttcaagt tagcttcatt
    17881 tatccgtgtt tggggactag ggattgctgc tttgttaatt tttatcgcag ttttcttgat
    17941 ttcaaatacc attcgtatta ccattatttc ccgcagtcgc gaaattcaaa tcatgcgctt
    18001 ggtcggagct aaaaacagtt atatccgtgg accgttcttg ttagaaggag cctttatcgg
    18061 tttattggga gctatcgcac catctgtttt ggtctttatt gtttatcaaa ttgtttacca
    18121 atctgtcaac aaatcgttgg tagggcaaaa tctatccatg attagtccag atttatttag
    18181 tccgttgatg attgccctac tatttgtgat tggggttttc attggttcat tgggatcagg
    18241 aatatccatg cgccgattct tgaagattta ggtaaaatag ctgcttttat gaggagattg
    18301 taaaatctcc ttttttgcta caagagtttt tgaaaagaga tgcgcagaag aaaagagctt
    18361 ccaaagaagt cccccagaga agacttct
//
LOCUS       DAVHEW010000035        18164 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVHEW010000035 DAVHEW010000000
VERSION     DAVHEW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18164
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.035"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..814
                     /locus_tag="VVL53_001090"
                     /pseudo
     CDS             1..814
                     /locus_tag="VVL53_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249392.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(889..1815)
                     /locus_tag="VVL53_001091"
     CDS             complement(889..1815)
                     /locus_tag="VVL53_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183276.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(1826..2893)
                     /locus_tag="VVL53_001092"
     CDS             complement(1826..2893)
                     /locus_tag="VVL53_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183277.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(2902..3828)
                     /locus_tag="VVL53_001093"
     CDS             complement(2902..3828)
                     /locus_tag="VVL53_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183278.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(3828..5324)
                     /locus_tag="VVL53_001094"
     CDS             complement(3828..5324)
                     /locus_tag="VVL53_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183279.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPAAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(5391..7370)
                     /locus_tag="VVL53_001095"
     CDS             complement(5391..7370)
                     /locus_tag="VVL53_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4183280.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(8057..9250)
                     /locus_tag="VVL53_001096"
     CDS             complement(8057..9250)
                     /locus_tag="VVL53_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4183281.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(9388..10830)
                     /gene="gtfA"
                     /locus_tag="VVL53_001097"
     CDS             complement(9388..10830)
                     /gene="gtfA"
                     /locus_tag="VVL53_001097"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU4183282.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTIEIETPTVHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            complement(11079..11912)
                     /locus_tag="VVL53_001098"
     CDS             complement(11079..11912)
                     /locus_tag="VVL53_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4183283.1"
                     /translation="MKKEEKLNQFWKYVLLIVGGILILVPLLVTVFSSFKTTKDIMNH
                     FFGLPNPFTLSNYERLVSDGIGGYFWNLAVITVLSLIVVAFFIPAAAYSIARNMSKKK
                     AFAIMYSLLILGIFVPFQVIMIPITVMMSKLGLTNMWGLVLLYLTYAIPQTLFLYVGY
                     IKISVPDSLDEAAEIDGADRFTTYRRIIFPMLKPMHATTLIINALWFWNDFMLPLLIL
                     NKDSKMWTLPLFQYNYQGQYFNDYGPSFASYIVGIVTITVVYLIFQKHIISGMSNGAV
                     K"
     gene            complement(11929..12801)
                     /locus_tag="VVL53_001099"
     CDS             complement(11929..12801)
                     /locus_tag="VVL53_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4183284.1"
                     /translation="MAIRKFLNKYWGWTFLLIPLALQAIFFYFPMVQGAFYSLTNWTG
                     LTYNYKFVGLNNYKLLMIDGKFFTAIAFTLILTLALIIGEITIGMVVARALNSKMKGQ
                     TFFRAWFFFPAVLSGLTVSLIFKQFFNYGLPTIGKILGISFLQESLLGTPIGAVVATI
                     FVLLWQGVAMPIILFLAGLQSIPSDILEAASIDGATSKQTFWKIELPYLLPTISMVFI
                     LALKSGLTAFDQIFALTSGGPNNATTSLGLLVYNYAFKSNQYGYANAIALILFLIIGI
                     VSFIQIKLSKKFEI"
     gene            complement(12820..14070)
                     /locus_tag="VVL53_001100"
     CDS             complement(12820..14070)
                     /locus_tag="VVL53_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019313951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4183285.1"
                     /translation="MKWYKKAGFLLVAGASLLGLAACGQNNQSTDGKITIEFFNQKTE
                     MADTLQKIVDNFEKDHPTIDVKLTTVPAAGIVLKTRILSGDVPDIVNIYPQNMDFQEW
                     AKAGYFADMTGKSYLENIKNDYAEKYAINNKIYSVPLTANLYGIYYNKTKFKELGLEE
                     PKTFKEFQEIVKKIKDRGNSPFAVAGNEGWTLNGYHQLSLITITGSGDAANNYLRFSK
                     PNAISVDDTILKADAERLDLLADNAQDGWRGASYNDAVVAFSNEKALMMPQGSWALAA
                     INQQDPKFDVGMFAFPGEEVGKDVTVGAGDMALSTSATTKHPKETEEFISYMTSPKAM
                     QAYYDVDGSPVAVKGVQEKEDSALAEISKLAFTDKHYVWLGQHWNSEEDFFNLSAGYL
                     MDKNLKNMANNLNAFFNPMKADLD"
     gene            complement(14105..16267)
                     /locus_tag="VVL53_001101"
     CDS             complement(14105..16267)
                     /locus_tag="VVL53_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU4183286.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDKEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYKNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     ELQENGEVYSGAELMYAGLTVILSQGDFLSKQYIFRRL"
     gene            16375..17235
                     /locus_tag="VVL53_001102"
     CDS             16375..17235
                     /locus_tag="VVL53_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4183287.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            17232..>18164
                     /gene="birA"
                     /locus_tag="VVL53_001103"
     CDS             17232..>18164
                     /gene="birA"
                     /locus_tag="VVL53_001103"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEU4183288.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKK
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
BASE COUNT         5880 a         3812 c         3090 g         5382 t
ORIGIN      
        1 agatgagctt tattgcgcaa gattttaaca atttcaatat catcactgtt cttgaaggta
       61 gaacacaagc tgtcatccga gatcactttc ttaaatatga tagagccgtc cgatgtcgcg
      121 tcaaaatcat tactatggct atgtttagcc cttactatga cttggctaaa cagcttcgct
      181 ttcaaatttc taagctcagg ctgaaacagt ctcccaggct gtttcactcc cgaatgctaa
      241 aatcgttctt gatcgctttc acattgtaca acatcttagc cgtgctatga gtcgtgtgca
      301 tgtccaaatc atgaatcagt ttcatcgaaa atcccatgaa tacaaggcta tcaagcgcta
      361 ctggaaactc attcaacagg atagccgtaa actgagtgat aagcgatttt atcgccctac
      421 ttttcgcatg cacttaacaa ataaagaaat tcttgacaag attttaagct attcagaaga
      481 cttgaaacac cactatcaga tctatcaact cttacttttt cactttcaga acaaagaccc
      541 tgagaaattt ttcggactca ttgaggacaa tctgaagcag gttcatcctc tttttcagac
      601 tgtctttaaa acctttctca aagataaaga aaagattatc aacgcccttc aactacacta
      661 ttctaatgcc aaactggaag cgaccaataa tctcatcaaa cttatcaagc gcaatgcctt
      721 tggttttcga aactttgaaa acttcaaaaa acggattttt atcgctttga acatcaaaaa
      781 agaaaggacg aaatttgtcc tttctcgagc ttagctgact tcaacccact acagttgaca
      841 aagagcctaa tttccataaa aattgacatg gaaattataa aaccattact agtttagtcc
      901 tttttgataa cgtgccaatt cggcttggtt cgcccaaaca tagtgacctg gacggatttc
      961 taccatagat ggcttatcag tctcatagtc gtgttgactt ggatcgtaaa ccttcaagac
     1021 cttcttacgt tccaagattg gatctgggat tggtaccgct gaaagcaagg cttgagtata
     1081 tgggtgaatt ggattgttaa acaattcttc tgtttctgca acctctacaa taacaccctt
     1141 gtaaataact gcgatacgat ctgaaataaa gcgaacaacc gacaagtcat gggcgatgaa
     1201 gagataggtc aggccgagct ctttttggaa ttttttgagc aagttcaaga cttgggcacg
     1261 tacagaaacg tccaaggctg aaattggctc atctgcaata acaaagtctg gttgcatgac
     1321 caaggcacgg gcaataccga tacgttgacg ttgaccgcct gagaattcat gagggtaacg
     1381 agtcaagtgc tcagcaagaa gacctacttc acggataata ttttgaactt tctctttacg
     1441 ttcttcttca tccttaaata aacggtgatt gtaaagacct tcagaaataa tataatcaac
     1501 agtcgcacgt tcattcaaac ttgcggcagg gtcttggaaa atcatctgga ttcgacgaat
     1561 caattccgca gcttgttcac gcgatttctt accattaatc ttttgaccat caaaaatgat
     1621 atctccatta cttgtatcat ttagaccgat gatagcacga ccaatagttg ttttcccact
     1681 accggactca cctacaagcg agaaagtttc tcccttgttg ataaagaagt tagcattttt
     1741 aaccgcgaca aacttcttac ttccttcacc gaaggaaatt tctaaatctt tgatttctac
     1801 taatttttca gacatttcct tcctcctagt cagccagatg ggcaaatccc attttttcac
     1861 ggatcttatc atggagattt gcaatcacag ctggtttttc tactttcgga gcatcctcat
     1921 gaagaagcca agttttagcc caatgtgtct ctgatactga gaattgagga gctttttgtt
     1981 cgaagtcaat ctgcattgcg tagtcagaac gcaaggcaaa agcatcccct ttcaggtcag
     2041 tataaagtga cggaggtgtt cctgggattg agtaaagatc ccctttatca tcagcaagct
     2101 gaggcaagct agacaagaga ctccatgtat atggatggcg agggtcatag aagacttcct
     2161 caaccgttcc atactcaacg atttctcctg catacataac cgctacctta tccgcaatac
     2221 ttgccaccac accaaggtcg tgggtaataa agattgttgt gaaatgatac tcgttttgta
     2281 aagattttag caaatcaata atctgagctt gaatagttac atccaaggca gttgttggct
     2341 catcacagat caagacatca ggtcggcagg caagggcaat agcaataacg atacgttgac
     2401 gcattcctcc agaatattgg aatgggtatt cattaaaacg tctatctgcg tctggaatgc
     2461 caaccttatt catgtagtca atggccaatt ctttcgcttc tttagctgtt tttccttggt
     2521 gttttacaat aacttctgta atctgactac caattgtttt aatggggtcc aaactagtca
     2581 ttgggtcctg gaagatagtc gcaatcttag caccacgaat ttgttcccaa tccttgtgag
     2641 aagataaagc tgtcaagtcc tgaccacggt agtcaatact accttgggca atacgaccat
     2701 tttcttcgag catacctgtg aaggtctttg tcaaaacaga tttacctgat cctgactcac
     2761 ctaccaaggc taatacttct ccttcgacta gttcaaggga aacgccgcga atggctgtca
     2821 atactttgtc acgaacgtca aattccacga caatatcgcg agcagtcaaa attacatttt
     2881 tttcttttgt catttctact cctatctatg tgtacgtgga tcactagcat ccgctaagtt
     2941 ttgaccaact acgaaaaggg acaaggatac caagacaagg gttgtcaatg gaatccagaa
     3001 caagtaagca ttggttgtta cgttttgtga ataatccgaa atcaaacgac ccaaacttgg
     3061 cactgtaatc ggtaatccaa gaccgaagaa agacaagaag gcttcgtatg agataaagct
     3121 tggaagcatt tgagtcatgg ttgtcacaat aacagatacc aattgaggca tgatattttt
     3181 ggcaacaatc ttcaaggttg gtgttcccaa agtacgtgac gccaagttgt attccaagtc
     3241 acgatagcgc aagatttgca cacggatcat gaaggcaata ccaatccatg ttgttacgct
     3301 catggcaaaa atcagattcc agaatccagc tccgattgag taagtcaaga caataacaat
     3361 caaaagaggt gggatgtttg agatgacgtt gtaaacttcc atcatgacac ggtcaactga
     3421 ttttgaaata ccccaaatac caccgacaaa aacaccgata accaagttaa tcactgtcgc
     3481 aatcacagaa atgaggatgg agttacgagc tccgaaccag acaccgtcaa agagcgattt
     3541 accgttactg tcagtaccga accaatgctc cgcatttggc ttgatataac gaacactaaa
     3601 gtcgtttacc ttgctgacat cattgaaatc aaacttagaa aacattgggt agatgaaact
     3661 tatcaaaatg atggctacca agattcccaa catgactaca gttgattttt tcttcataaa
     3721 ttgtttaaac actgatttcc agtaagaata tgctggcgca tcaatagttt cagaggcaaa
     3781 atcgtcacgt tttacaaact gaaatttttc tttatcgatt gtagacatta tttgcctcct
     3841 ttctcagtca atttaatacg tgggtcaata atagtcatcc aaatatctcc caaaagacgt
     3901 gagaagatag aaatacatgt aaagatgaag acaagaccaa cgaccataga gttattagat
     3961 gcttttacag agtcaatcaa cattttaccc atacctggga aggcgaagac tgtttcagta
     4021 agggttgcac caccgataac cccaataatg gcagcaggaa ttcctgaaac cagcggaacc
     4081 atggcatttt taaagatgtg tttgtttgaa atttcttttt cagacaaacc ttttgcacga
     4141 gcgaaacgaa caaagtcttg agattgcaag tcaatcatgt aacgacgaat ccaaatggct
     4201 gtaccaggag cacccaacaa accaaggatg actgctggta aaacgtaaga acgccaatct
     4261 ccagctccca agatagggaa tgaatctgga agggcaatag atgatccaat caatcgaacg
     4321 atgtaaacca aggcaatcgt tggaagagca agcaagaagg tcaaagcccc tgttgagagg
     4381 ctatcaatcc aagtgttctt gaaacgagcc atggctgaac caagtggcac ggcaagagca
     4441 taggcaagaa ccaaaccaat caaaccagta atagcagagc tgacaatcat agatggatat
     4501 tggtaattac tttcagtcgc tgtataagga tcatctttcc catagctagc tacttcacga
     4561 gagtcagcct gactaggtga cttgtaggtt cttgagtaaa tatttacaga agacgttttc
     4621 ttacctgttg ggaactgaac ttgggcagtt ttggtttgtc cttgaccttg agtaataacc
     4681 tgaagaactg gtgtattagc ataggttggg taagagtcac ctaaattcaa gttcacaaag
     4741 ttttgatgaa caaatgggaa ctgactgtta aagtacaaga gatatttatg tttagttcct
     4801 gaaccgacca atgaccatcc gatagctgga tcattttcaa aacgaaggta gcgtttcaag
     4861 tctggatttt cagggtcttg gattttattt gtatggtcaa tgtcaatcaa gttagcatag
     4921 aagtgaaaaa cacgttcaaa aattggaatt tcacgagtag catagaattg accactttca
     4981 gtaaattctc ccaaagtcca accatgacct aattgattga tgtacttttc ataaatagct
     5041 ttattggtcg catttgcttc tactgttaca gaagaatcca tgctacttgc cttttcttgc
     5101 aactctttag tatcgtaata ctcaatgtag cccatacgct caaacacagt attttcatag
     5161 ttatcacgtt tatcagccgt tgtcgcaatt ttattatagt taggatcctg cttgaaaatc
     5221 aattttcgag gaaccaaggt atagataatc gtgtaggtca aagtcgttac taagaaaatc
     5281 gaaaccaatg accgcaaaac acgcataaaa atatattttt tcatattatt tcctttaaaa
     5341 atcccaaaag aaccttctcc tcatggagag aaagttctat tagaaattat ttacttcaca
     5401 tgacttgcca attctttttg agctttctca tttgattcag ctttttcttt caaccatttt
     5461 tcacgagctt tttcatactc ttccttagtc accactttat cttgtaattt caaatatttg
     5521 aagtaaacat ctgacccctt agagcctgtt tgcgcagaag ctccagtaaa tggaacaatt
     5581 cgtgaaagca ctggtgctgc accagaagaa gccatagcag gaataaagag tgaactatct
     5641 gtcaaccatg cttgagccgc tgcatatttt tcataacgga cattcaagtc gcttgtctct
     5701 ctggcagctt catcaactaa tttatcgtat tctttcaaac caacttgaac tactgaaggg
     5761 ctatttggat tatcaaatcc taaatatgtt tttgtagttt cactgctagt tgtttttaaa
     5821 atatccaggt aagtagatgg gtcttgatag tctggccccc atgaaactcc tcctgataca
     5881 tcccaatcct cagatgaagc attggcagca tagtaagtaa tattaaggaa ttcatcactt
     5941 gtcatttgtt gaatatcaac aacgacattt tcaacaccaa gaactgtttc tacagattgt
     6001 ttaaaggact gaatacgaga tatgtagttt tttgatgctt ggtctactgg aacgtccaga
     6061 tgaataggaa actgaacgcc gtctgcttct aaagctttct tagctttcgc aaactctgcc
     6121 ttggccttgt cagcattgaa taaaccatcc tgcccatcag ctaaattcac acctttccac
     6181 tcatcaccat aagcaggaag ttgagcagcg actaaatcac caaaggtctt ctcaccagct
     6241 gaaacaaagt ctggttttac aaataaatta cgaactgcta aagctgctcc atctttacca
     6301 ttgatttgag ctgagtaagc tgagcgatca agagcaaaat tcaaggcttg acggaaatct
     6361 ttgttaagca atgccttctt agtagctact ttctctgaat ctgtagtttt agaagtatag
     6421 ttgtaacttt ggcgatcaat attcacaccc agaccagcaa tcccagagcc tgattgtgtg
     6481 taatagatat tgtccttgta ttcttctgca accttagaat agttggagct ggtagggtaa
     6541 agacgggcat aactataagc tccactagtg aagttacgct ctagcgactc ctgatctgat
     6601 ccatcatagt aagctagatt gatagtatct aggtggacat tttctttatc ccaatattgc
     6661 tcatttttta caaactctac agaagatttt gcagtcaacc ctttcaacaa gaatggacca
     6721 ttataaagca aggatgtcgg atctgttggt ttagcaaaat cgcttccttt tgatgtttcg
     6781 aattcttcat tcagaggcca gaaaatagaa taggtcaact tagagttcca gaacggttca
     6841 ggctggttca aagtgtattg taacgtataa tcatcaaccg ccttgacacc aactgttgaa
     6901 aaatctgttg aagttcctga tagataatct gccaagcctt taaccgaatt ttcagctaaa
     6961 tacatagctt ctgatttttt atctgctgcg tgttttaaac cgttcacgaa atctttagcc
     7021 gtcacctctg catattcttc tccatcagag gtaaaccatt taaccccttt acgaatctta
     7081 taagtgtagg tcaaaccatc cttagagact tcccaatcct ctgcaactgc aggagcaaga
     7141 ttaccgtaat tatcgttagt gaataaacca tcaatcccat ttgaagtcac tactgttgta
     7201 ctatttttac ttgaaatcag gtagtccaag gtttctgggt ctgctgtata aacatagcca
     7261 taagctttag gggctgatga atcagatgat tttgaagaac tgcatgctgc aagtacacct
     7321 gctgctaata aaacaagacc tgctgtagca aatacacgat tttttttcat tttctactcc
     7381 tctgtttatg tgaattatag attgacaacc attatatcac attatccatt aaaaatcaaa
     7441 caaattttca gaatatttag gcttgttggc acaaattttt catttttttt tgaatatatg
     7501 attcaaattg tcgttcgaag tgtcaaagac tacagtgaaa ataggaaatt tgacgcagaa
     7561 actttggagt ttaggaagac atacagtaaa atgaaatacg gacggaacaa tgtgattttg
     7621 gaattcaaat taaattataa caatattgta gaagtatcat tctagtattc aagattcagt
     7681 ttactatgtc ttttcacacc aaccttattc cgaattcaat tacttttgtg atttacatat
     7741 atagattaag actatctttt atactttaaa atttctcgct accttatcca ctatatgctc
     7801 ctcgctatca cgtttctatt catagcctac gatttcacta ttgctttctc tgacaattct
     7861 tatttcctgc gtcagactta aaacgatcta tccccagacc attttaatcc gctacctcac
     7921 gatagtcagg cttggggagc gctattgtat tcaccggtag tggatcccta cagaggactt
     7981 acacctcaga tgcacgacat gcccatcgta taaaaaaatc tcctacccaa ggtagaagat
     8041 ttcaaactta taaaacttaa tccgtcatgt ccgataccaa cattcgatgc tccaatggaa
     8101 tactgcacat aactagcaag aaaataaagc ctgactgaat ccagaagaga gccaagtcaa
     8161 aaattccgtg cacagcaacc actgtaagga aagatagata aaggccgata atcggacgtt
     8221 tccccgactc ctgactcata tccatcatca agcgaacagg agcaacagaa gacaaaacta
     8281 ataaaatggt acccacaatt ccgtaactca gaatcgtatc aatataaaga ctgtgggcat
     8341 gttcatgata aggagcatgt atccgaggat aagagtgcat ataggtcaat ggcccttcac
     8401 cccaaaaagg attttgctta aacaaggcca tcccagcatc ccagatagaa atgcgttctt
     8461 ccatagaaga gtctaaagta cccattcgaa ctcccaaatc actagaaaag aggaaactca
     8521 aaccaatcgc gaagacccca atactaagcc aaaaggcctt ccagttttta atagtcgtaa
     8581 agagatagat aattgctcca gcgataatag caggaaaggc agttcgattt tgagtaaagt
     8641 tcaaaccaaa gagattaaca aagcctgcaa tcacacagaa tactttcaac caattcaact
     8701 tggtcgttgt aaacagatag aaagcaatca taatacagaa acaacaaata attccataat
     8761 aattaggatt aaagaaagtc acttctgccc ggttctgatg ccacacctgc atattgggtg
     8821 aaagaaaagc atagttaaat ttcttcacaa tttggaaatg ttctaaactg gcaaaagcag
     8881 ctgacaagac actaccaaac aagacgaact gcaaaatcaa tcgaaagaat ttatgggata
     8941 aaatcgactg atagtgcaaa aagaaaatag taaatagaaa cattcctact gaagccacaa
     9001 gacccatcca attttgtgca agaatggata taacagtact atagctaaga aaaagaagca
     9061 gcatcggatg ctcccccatt ttctgaagaa tacttttcat gtctcctgta aaaatcaaac
     9121 tgataatata taaacagagt acaactacaa aaagataaaa gggtaaaaag atacttagga
     9181 taattcccaa taaaatcagc tctttactag acaacccctt cagcttttca ataaagccta
     9241 ttgatttcaa aatgaatcct ttctctccaa atcagctgat tcagataata gtaagctatc
     9301 ctatattgta ccactttttt agcaatttga aaacaaagga aacgttttcc aaaataaaaa
     9361 ccctatttta tccaccatat caaggcttca aaatgttact tcaactccat tctcaattac
     9421 ccgataagtc tgattttgca aatcaatttc tgctactgct gttacggact tatctttatt
     9481 ttgacgtttg attacaatgc tgtgaactgt tggtgtctct atctcaatag tcccttctag
     9541 atcaaaggct tctgaacggt tacggaaaga aaatagattg agaagggcct tcacaacaga
     9601 tcgttgcact tcttttgcta tttcctcgtt gctatagtaa tgacgattaa tatttcgacc
     9661 ttctttagtt tcttctaata atttcaagtc attcttgcct gctaatagac ccacatagta
     9721 aacctgagga atacctgggg caaaagcttg aattagacga gcgagaaaat acttgacatc
     9781 atcatctcca agcgctgaat agtaggttga attgatttgg tagatatcta agttgttata
     9841 ctcggcacta gagtacttac gtttgacatt ggctccaacc ttatagagtt catttgaagc
     9901 atagtcaatc tcctcatcgg tcaggatatc cttgacatct actactccaa tcccatcatg
     9961 ggtatctagc gtcgtaaatt gcttcatcgg gctcatcttt aaccacttag ccaaacgctc
    10021 tgttctggaa ctgtaaagag tataaagtgt caccattgga agagcaaaat cataaacata
    10081 gtaatcatgg tctgctattt taaactgaat cgaatagtgt tcatgaatct caggtaaaag
    10141 ctctgtccca tactcagcag cgatatctcg aactttgtcc aataaatccc aaatatctgg
    10201 ttccacaaag aaatcattag tatccaattt cttcactgca taagcaaagg catctagacg
    10261 aatcaaatca cacccattac ttgccaagtg ctgaatggtc ttacggataa attccatagt
    10321 tacttctttg gtcacatcaa gatcaatctg ctcctcacca aaggtattcc acaaatgttc
    10381 cactgaacca tcttcaaaca caatctcttg ctttggtgca cgatccttac gcttgtaaat
    10441 taaatctaca tcagactgtg tcggacggtt ttctggccaa aacttatccc agtttaaaaa
    10501 gagatcttta aattcactgg cttcatgttt ttcttgatag tccttataat acttggattg
    10561 acgagaaata tgattaatca taaaatcaaa cataagataa tatctctcac ctaaacgctt
    10621 aacatcctcc caatcaccaa aagctgagtc cacttcgtcg tagtcaactg gcgcaaatcc
    10681 acgatcacct gttgatggga aaaatggtaa aaggtgaact cctccaatag catctccaaa
    10741 atgctcttcc aaattatcat ataagtcttt aagattattt ccaaggctat cagaataggt
    10801 aatcaacatg gttttattct gaattggcat cattactctc ctttttttaa ttgaagccaa
    10861 gtctcatatg acttggcttt tatgagttta tgtattttaa gaattgcttc atagggtacg
    10921 attgaaatca gcaagctgaa tcgtcccttt gtatacggaa ttgaacacgg gactaatttc
    10981 ttaggaaaaa agataaatct tcttgtgtgc aagcacacta cgtcaatttc ctatttttct
    11041 acagaaattt tcagcaagct gaatcgtccc tttgtatctt atttcactgc tccgttgctc
    11101 attcctgaaa taatatgttt ttggaagatg aggtagacga cagtaattgt tacaattcct
    11161 acaatatagg atgcaaaact tggcccatag tcattaaagt actgaccttg gtagttgtac
    11221 tggaagagag gcaaagtcca catcttggaa tccttattca aaatcaacaa tggcaacata
    11281 aagtcattcc agaaccataa tgcattgata atcaaggttg tagcatgcat gggcttcagc
    11341 attggaaaga taattcgacg gtaagttgta aatcgatcag ccccatcaat ttctgcagct
    11401 tcatctaaac tatctggtac actgatttta atataaccaa catacaagaa gagggtctgt
    11461 ggaattgcat aagttagata aagtagtacc aatccccaca tatttgtcag gccgagtttg
    11521 ctcatcataa ctgttatcgg aatcatgatc acctgaaatg gaacaaatat ccctaagatc
    11581 aagagcgaat acataattgc aaaggctttt ttcttggaca tatttcgagc aatggaatag
    11641 gctgcagctg gtataaagaa ggctactaca atcaatgata acaccgtaat aacagctaaa
    11701 ttccagaaat agcctccaat cccatccgaa accaatcgtt cataattgct taatgtaaaa
    11761 ggattgggca aaccaaagaa atgattcatg atatccttgg ttgtcttgaa ggaactaaaa
    11821 accgttacca acaaaggaac aagtatgaga ataccaccta ctatcaagag gacatacttc
    11881 caaaactgat tcaatttttc ttcttttttc atgtgtaaga ctcccctatt agatttcaaa
    11941 tttctttgat agtttgattt ggataaaaga aacaattcca ataattaaga ataaaatcaa
    12001 ggcaattgca ttcgcatatc catattgatt actcttgaag gcatagttat agactaaaag
    12061 accaagagac gttgtagcat tattcgggcc accactcgtc aaggcaaaaa tttggtcaaa
    12121 ggcagtcaaa ccagacttaa gagctaggat aaaaaccata gaaatcgttg gtagcaaata
    12181 tggtaattca atcttccaaa aagtttgttt gcttgttgca ccatcaattg atgccgcctc
    12241 caaaatatca cttggaatac tctgaagacc agcaagaaag agaataattg gcattgctac
    12301 tccttgccat agaagaacaa aaatggttgc tactaccgca ccgatcggtg ttcctaatag
    12361 actttcttgt aaaaaactaa tccctaaaat ttttccaatc gttggaagac catagttaaa
    12421 gaattgttta aaaatcaagg aaactgtcaa accagacaaa accgctggga aaaagaacca
    12481 tgctctaaag aaggtctgcc ctttcatctt agaattcaag gcccgagcca caaccatccc
    12541 aattgtaatc tctccaataa tcaatgccag tgttaaaatc aaagtaaaag caatggctgt
    12601 aaagaatttc ccatcaatca taagcaattt gtagttattc agaccaacaa atttataatt
    12661 ataggtcaat ccagtccagt tagtcaaact atagaaagca ccttgtacca tcggaaagta
    12721 aaagaagata gcttgtaatg caagcgggat gagcaaaaaa gtccaacccc agtatttatt
    12781 taaaaatttt cgaatagcca tatcatctta actcctattc tagtccaaat ctgccttcat
    12841 tggattaaag aaagcattga gattgtttgc catatttttc agatttttat ccatcaagta
    12901 acctgcactt agattgaaaa aatcttcttc agaattccag tgttggccca accaaacata
    12961 atgtttatcc gtaaatgcta gtttagaaat ctctgcaagc gctgaatctt ctttttcctg
    13021 tacacctttt acagcaactg gtgatccatc cacatcataa tatgcttgca tagcttttgg
    13081 actagtcata tagctgataa attcctcggt ttctttagga tgtttggtag tagctgaagt
    13141 tgataatgcc atgtcccctg caccaacagt aacatctttt cctacttctt ctcctgggaa
    13201 ggcaaacatc cccacatcaa attttggatc ctgttgatta attgccgcta atgcccatga
    13261 tccttgtggc atcatcaagg ctttttcatt cgagaatgct accacagcat cattataaga
    13321 ggcaccacgc catccatctt gagcattgtc tgccaacaaa tctagtcgtt ctgcatctgc
    13381 ttttaaaata gtatcatcta cagaaatagc atttggtttt gaaaagcgaa gatagttatt
    13441 agctgcgtct ccactgcctg taatagtaat gagagaaagt tggtgataac cgtttaatgt
    13501 ccatccttca ttgcctgcaa ctgcaaacgg agaattccct ctatctttta tctttttaac
    13561 aatctcttga aactctttaa aagtcttcgg ttcctcaagt cctaattctt taaactttgt
    13621 tttattgtaa tagattccat aaaggttagc agttaaaggt acactataaa tcttgttatt
    13681 gattgcatac ttttcggcat agtcattctt aatattctca agataggact tccctgtcat
    13741 atctgcaaag taacctgctt ttgcccactc ctgaaaatcc atattttgag gatagatatt
    13801 aacaatatct ggaacatctc ctgataaaat ccgagtcttc agaacaattc cagctgccgg
    13861 aacagtcgtc agttttacat caatagtagg atgatccttt tcaaaattat ctacaatttt
    13921 ttgcaaagta tccgccattt cggtcttctg gttaaaaaat tcaatcgtta tctttccatc
    13981 agtcgattga ttattctgac cacaagctgc taggcctagt aaactagccc cagctactaa
    14041 aagaaaacct gctttcttat accatttcat tggaaagcct cctttataaa tttatacacc
    14101 tttattatag tcttctaaaa atatactgtt tactcaaaaa atctccctgg gataagataa
    14161 cagttaaacc tgcatacatg agttctgcac ctgagtaaac ttcgccattt tcctgtaatt
    14221 catacagtcc ctcttcatcc aaatctttta actttaaagt tgtttccatg gtctctacaa
    14281 ctgataaaac gcgaacgtag gttacaatcg tttgattttt gtaattaaat tgtacagctg
    14341 cttcattgga ggcagtatca ggattaatta gtctatactg ctgtcctaac tgaactactg
    14401 gtcgtaattc tttatacaag ttcacctgat tagcaatcgt agctttctct ttatctgata
    14461 aatttgtcaa atcaagctca tagcccaaat ttcccatcat tgctacatgg ccacgtgttt
    14521 ctaatggtgt cattcgtccc atctgatgat tcggtactgc tgacacatga gcccccatag
    14581 aaatggttgg atagagatag gatgaaccgt attgaattgg taaacgtgca atggcatcag
    14641 tattatcact agcccagact tgtgggaaat agcgcatcat accaagatca tttcgtccac
    14701 caccaccaga gcaggactca aagagaatat ggctgtgctt ctctgtcaga taagaaacga
    14761 gttcgtaaag ccccagcatg tactgatgag attgcatctg tgtctctaga taagttaatc
    14821 cattccctag cttagtgata ttgcggttca tatcccattt aatgtaatca atctcatgat
    14881 aagataggag ttgatctaag acacttttca agtattctac tacctgagga ttggcaagat
    14941 taagtactaa ttgattccga gaataagtat gctcatagcc aggaacctga atagcccagt
    15001 cagggtgttg acgatacaaa tcactatcta cagaaatcat ttcgggttct aaccaaagtc
    15061 caaactgcaa acctctttca tggatagctg aaatcagact ttctagactt ccacccagtt
    15121 tttcctcatt aacaacccaa tcacctaaag cacgattatc atcaaaacga ttgccaaacc
    15181 aaccatcatc taatacaaaa agttcaatgc caactttctt agcttcatct gctaactcta
    15241 acagtttttc tctctgaaag tcaaagtaag tagcttccca gttattgatt agaattggac
    15301 gttctttttt agaaaattca cttagcataa tgtgcttcag tacaaaattc tgactttcat
    15361 gactaatacc agttaatccc tgatctgaat gggtcactaa agctaccggt gtttcaaagt
    15421 attcctcagg agctaacttc caagaaaagt tttctggatt aatgccaata gccacccgaa
    15481 cttcattcaa ttgatttttt tgaacaaaag cttcaaagtt gccactatac attagttgaa
    15541 tagcaaacac attcccagca tcctctgtga ctccttgttc gcatagtaga agagctggtg
    15601 tttgagcatg accagaagca cctcggtttg agctaatcga aaagattcct tgttctacct
    15661 gttgacgtct aacagtcttt tcacgagcat aagcaccctg cagagttact atttcgtaat
    15721 ctgcagctgg aaaatcagcc ataaaagaaa aatctttatg gatgacaact tcctgattac
    15781 tattattatc taatttactg tagctagcaa tagtcgcatc attattaaaa gtagtataat
    15841 acaaagtcag actaagttga gccttagaat cttctaacat taagacaaga gtctctgtat
    15901 cgtccatgct atgtggagaa ggtaagccct gtggaccatt ctgacctttt aaaatctttg
    15961 cttctacaaa tcgaaagtct gttacttcag ttacattatg ctgaacctgt atggttggtt
    16021 tcctaaaatc tcctaagcca tgttgtccaa aaatctgtcg ctgagtatct aaactaaagg
    16081 ttcgattagt agccgttgga tttcctgaaa aggcatggtc tcgttcataa acactattgg
    16141 aacctttata gttcttaata gtctttccta aatgtttcaa aagtaagtag ccatttcgat
    16201 tttcaataat caaacttaga tttttactct caacataaaa tagattattc tctatcctaa
    16261 ctcccattta cttcacctca tcactttatt gattatattt tatcacctga aaccgctttc
    16321 caaaatagaa aaatgtctca agaatatggt aaaatgttag gtaggaggta gcacatgcta
    16381 gttttttcag aataccagac tggaacaatt gaccttgccc taagctttta tggatatgag
    16441 gaatgcacac ctaattactc ttttggtcca gccattcgtg atacatacgt cctacattac
    16501 attactaaag gacaaggaaa atttcattac aagggtaaaa ttgttgattt aaaagaagga
    16561 gatttctttc tattaaaacc agaggaacta accttttatc aagcagatag taaagaacct
    16621 tgggcctact actggttagg aatcactgga gggaaagccc ctgattattt tgctctttcc
    16681 caaatttctg atcaatccta tctcatccaa tctgaaactt gtcataccca gactactgca
    16741 aaactcatct cagacattgt ccgcttcgct cagattacaa aatcaagtga attagctcaa
    16801 ctccatatca tgggacaact tcatgaactg atgtttcatc tgggaactat tgctcccaat
    16861 cagaaaaaaa agaatatttc atcaacccac caactctatc ttgaatgcaa acgattaatt
    16921 gatagccact atcctcaatc acttacaatt caagatttag caaaagaact atccgttcac
    16981 agaagctact tatcaagcgt attcaaagaa tttaatacct tatcacccaa agaataccta
    17041 ctctacgttc gaatgcaccg agctagacaa cttctcgaaa atacccaaga gtccatcaag
    17101 gtaattgcat actcggtagg tttttcagat ccactccatt tttcgaaagc ttataaacaa
    17161 tactttaatc agactccaag tcatacaaga aaagaatact ctcaatacca actagtaaga
    17221 aaggcaacat tatgaaatcc taccaagctg tctaccaaat cctatctaaa gaaaccgact
    17281 atatcagcgg agaaaaaatc gcagaaaaac tatccctaag ccgaacagca atttggaaag
    17341 ccatcaagcg actagaacaa gaaggcattg aaattgatag tatcaaaaat agaggatata
    17401 aactgatgaa tggtgacctt attcttccag agattctaga agaaaatctt ccaattaaag
    17461 tcagctttaa acccgaaaca aaatcaacac aactagatgc aaaagaagca attgatttag
    17521 gccatgaagc aaataccctc tatctagctt cctatcaaac agcaggccga ggccgttttc
    17581 aacgttcctt ctactcacca caaggtggta tttatatgac actccatctt aaaccaaatc
    17641 tcccctatga caaattacca tcctacacac tacttgtagc tggagctgtc tacaaagcca
    17701 ttaagaacct aactttaata gatgtcgaca taaaatgggt caatgatatc tatctaaaca
    17761 atcataaaat tggaggaatc cttactgaag caatgacctc tgtagaaact ggcttagtca
    17821 cagatatcat tattggagta ggtatcaatt tcactattaa agacttccct caggaattaa
    17881 aaaaaaaagc tgccagctta tttaaagcta cagctcctat aacaaggaat gaattgatca
    17941 tagaaatctg gcgtgctttc ttcgaaacac cagcagaaga gctattatac ctatacaaaa
    18001 aacagtcatt cattctagga aaagaagtca ctttcacact agagcaaaaa gactacaagg
    18061 gacttgctaa agacatctca gaaaatggaa aacttttagt tcaatgtgat aacggaaaag
    18121 aaatctggct aaatagtggc gaaatttctc tcaatagttg gaag
//
LOCUS       DAVHEW010000036        18072 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVHEW010000036 DAVHEW010000000
VERSION     DAVHEW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.036"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(63..1685)
                     /gene="groL"
                     /locus_tag="VVL53_001104"
     CDS             complement(63..1685)
                     /gene="groL"
                     /locus_tag="VVL53_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU4183289.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1701..1985)
                     /gene="groES"
                     /locus_tag="VVL53_001105"
     CDS             complement(1701..1985)
                     /gene="groES"
                     /locus_tag="VVL53_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU4183290.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2141..2536)
                     /locus_tag="VVL53_001106"
     CDS             complement(2141..2536)
                     /locus_tag="VVL53_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4183291.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2614..3375)
                     /locus_tag="VVL53_001107"
     CDS             complement(2614..3375)
                     /locus_tag="VVL53_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4183292.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTMVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3417..4043)
                     /locus_tag="VVL53_001108"
     CDS             complement(3417..4043)
                     /locus_tag="VVL53_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU4183293.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4059..4376)
                     /locus_tag="VVL53_001109"
     CDS             complement(4059..4376)
                     /locus_tag="VVL53_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU4183294.1"
                     /translation="MIQPASLEELASLVEKAGKKVFIFVADWCSDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(4373..4672)
                     /locus_tag="VVL53_001110"
     CDS             complement(4373..4672)
                     /locus_tag="VVL53_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU4183295.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5009..5200)
                     /locus_tag="VVL53_001111"
     CDS             complement(5009..5200)
                     /locus_tag="VVL53_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU4183296.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5361..6035)
                     /locus_tag="VVL53_001112"
     CDS             complement(5361..6035)
                     /locus_tag="VVL53_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183297.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6041..6487)
                     /locus_tag="VVL53_001113"
     CDS             complement(6041..6487)
                     /locus_tag="VVL53_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183298.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6601..7104)
                     /locus_tag="VVL53_001114"
     CDS             complement(6601..7104)
                     /locus_tag="VVL53_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4183299.1"
                     /translation="MKNYQEWYRNISSRLTSCPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7101..7463)
                     /locus_tag="VVL53_001115"
     CDS             complement(7101..7463)
                     /locus_tag="VVL53_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU4183300.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7529..9388)
                     /locus_tag="VVL53_001116"
                     /pseudo
     CDS             complement(7529..9388)
                     /locus_tag="VVL53_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9427..9876)
                     /locus_tag="VVL53_001117"
     CDS             complement(9427..9876)
                     /locus_tag="VVL53_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU4183301.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKPVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10000..10716)
                     /locus_tag="VVL53_001118"
     CDS             complement(10000..10716)
                     /locus_tag="VVL53_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4183302.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11597..13012)
                     /gene="ply"
                     /locus_tag="VVL53_001119"
     CDS             complement(11597..13012)
                     /gene="ply"
                     /locus_tag="VVL53_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU4183303.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13023..13355)
                     /locus_tag="VVL53_001120"
     CDS             complement(13023..13355)
                     /locus_tag="VVL53_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183304.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13426..14034)
                     /locus_tag="VVL53_001121"
     CDS             complement(13426..14034)
                     /locus_tag="VVL53_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183305.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14047..14505)
                     /locus_tag="VVL53_001122"
     CDS             complement(14047..14505)
                     /locus_tag="VVL53_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU4183306.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            14630..15432
                     /locus_tag="VVL53_001123"
     CDS             join(14630..15080,15080..15432)
                     /locus_tag="VVL53_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU4183307.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLNLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANYSGKKKCHAMKAQAIVTSRGRFVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKAYNHA
                     LSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIFNHELGF"
     gene            complement(16170..16430)
                     /locus_tag="VVL53_001124"
     CDS             complement(16170..16430)
                     /locus_tag="VVL53_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183308.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(16489..17040)
                     /locus_tag="VVL53_001125"
     CDS             complement(16489..17040)
                     /locus_tag="VVL53_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183309.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(17030..17227)
                     /locus_tag="VVL53_001126"
     CDS             complement(17030..17227)
                     /locus_tag="VVL53_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183310.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(17231..18025)
                     /locus_tag="VVL53_001127"
     CDS             complement(17231..18025)
                     /locus_tag="VVL53_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183311.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
BASE COUNT         5374 a         4032 c         3234 g         5432 t
ORIGIN      
        1 aaaaagaagg gttagtcatt ccctcctttt gtgtttttta taagttgttt tctatagaaa
       61 gcttacatca tcccgcccat catgcttgga tccattgctg gagctggggc tactggttct
      121 ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc tggctacaga tgctgcattt
      181 tgtagggctg aacgactcac tttaactgga tcaatgatac cttgatcaat catgttaacc
      241 cactcgccag ttgctgcgtt aaatcctata ccaagctcag catttttcaa acgatcgata
      301 acgatagatc cttcaaatcc tgcattgtga gcaatttgac gaacgggttc ttccaaagca
      361 cggagaacaa tattacgtcc tgttgcttca tctcctgtca attccaaggt agcaacagct
      421 ggaatcacat tggcaagagc tgttccacca cctgcaacaa taccttcttc aacagctgca
      481 cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt ctttcaactc agtttcagtt
      541 gcggctccaa ccttaataac cgctacacca cctgacaatt tggccaagcg ttcttgcaat
      601 ttttcacggt caaattcaga agttgtagtt tcgatttgag acttgataac cgcaacacgg
      661 tgagaaatcg cttcaggatt tcctgcacct tctacaataa ccgtgctatc tttgtccacg
      721 gtcactctcg ctgcttgacc aagagcttca attgtcgcat ctttcaactc aagaccaagg
      781 tcttctgtga taactgttcc gcctgttaag atggcgatat cttcaagcat ggctttgcga
      841 cggtcaccaa aaccaggtgc cttgactgct actacgttga aggttccacg aatcttgttc
      901 aaaacaagag ttggaagagc ctcgccatcc acatcatccg caataatcaa gagtggacga
      961 ttgctttgga gaatgctttc caaaagtggc aagatttctt ggatattgga aattttcttg
     1021 tctgtaatca aaatgtacgg attttcaagg tcagccacca ttttttcgct atctgtcacc
     1081 atgtactgtg aaaggtaacc acggtcaaac tgcattcctt ctacgacttc aagctctgtt
     1141 tccataccac gtgactcttc gatggtgatg acaccgtctt tgccaacttt ttccattgct
     1201 tcagagatgt actcaccaac tttttcagaa cgagaagata cggctgcaac ttgagcgata
     1261 gcttctttat tggcaacagg gatggcgttg tttttcaaag cttcaactgc tgcggcaact
     1321 gctgtttcaa tcccacgacg aataccgatt ggatttgcac ctgctgtgac gtttttgatt
     1381 ccttcacgga cgatagcttg ggtcaagact gttgcagtcg tagttccgtc acctgcgata
     1441 tcattggttt tagaagctac ttctgatact aacttagcac ccatattttc aaaatggtct
     1501 tccaattcga tttctttggc aatggtcaca ccgtcattgg taatcaaggg tgaaccgaat
     1561 gacttttcaa gaacgacatt gcgacctttt ggtcccaagg ttactttaac agtgtctgca
     1621 aggatatcga caccacgaac catggctgaa cgggcatctg atgaaaattt aatttctttt
     1681 gacatactta ctttctcctt ctattcctca atgattgcca aaatgttagc ttcgcctacg
     1741 atgatgtact tttcatcgcc atctttgaca tcaagacctg cgtgggcttc aactaagaca
     1801 cgatctccag ttttaacact tggagcaacc aagtcaccgt tcaaggtacg aacaccttgt
     1861 ccagtagcca caacttgagc tgttttggtt ttttcttggg ctgagcctgc aaggacaaag
     1921 cctccaacag tttgttcttt ttcttctatt tttaagacca cacggtcccc taatggtttc
     1981 aacatctgat ttcctccata atgagataga tattattagc actctttgaa cttgagtgct
     2041 aattcatagt tctattgtat cacttggtca gaaataatca agaaaaaagt ctgactttct
     2101 caagataaaa agcctgagac caactcagac tttttaattc ttaaaatggc aattcttcct
     2161 cttccaagac caaatctgcc aaatcttggc ctgcattatt ttcacgcata gcacgttggg
     2221 cacgactttc caagagttgg aatcctgtga caagtacttc ggtcacgtag ttcatttggc
     2281 catttttctc aaagcgacgg gtacgcaatt ctccatcaac ggaaatgaga ctacctttgg
     2341 ttgcgtagct tgccaaagtt tctgctagtc tgccccatag gaccatattg acaaaatcag
     2401 cttcacgttc accgttttgg tctttgtaac gacggttcac agcgatagtt gctcgcgcta
     2461 ccgacttgtc attgttggtt ttgtgcaatt ctggtgtaga cgttaaacgt ccaatcaaga
     2521 taactttatt atacatattt tcttcctcct acttatctat tcgtaggaaa tcaaaaaaag
     2581 ttacagaaat ttgtaacttt tcgagaaaat tttttatttt ttatgaacca tgaaacctgt
     2641 cgcctgttga ttggccataa tggtcatatc tgtaatctga acacgacgag gttgactagt
     2701 cacatagact actgtatctg caatatcctg agcttgcaaa gcttctattc cttggtaaac
     2761 ggacgcagct cgttctttat caccatgaaa acgcactgta gaaaaatctg tttcgacaat
     2821 tccaggctga atggtcgtca ccttgatatc cgttgcgatg gtatcaattc gcagtccatc
     2881 tgaaaaggtc ttaactgccg ccttggtggc tgagtaaaca gctgcaccag cataggcata
     2941 aattcctgcg gttgacccca tattgataat atgaccttga ttggctttta ccattgctgg
     3001 caagaaacag cgagtgactg ccatcaaacc tttgacattg gtatccaaca tggtcagcat
     3061 atccaactct tcatagtctt gatagggagc taagccaaga gccagtcctg cgttattgac
     3121 caggatgtca atctgaccta tcgtttctaa aatatcagag cagacagtct ttaccattgt
     3181 catatccgtg acatctagga gaaaagtcca aactgtttga tttggaaaag tttctgcaaa
     3241 ctccgcctta agagcttcta gtctgtctat ccgtcgtcct gttagaacga catcctcacc
     3301 ctgctccaga taagcacgcg caatcgcttc accgattcct gatgtcgctc ctgtaatcac
     3361 aacatttttt gccatcttat ttccttctag ctggtctatc agatattaac aacttcttag
     3421 gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     3481 agcaccccac gtttttgtgg agcatttggc agatgcaatt cacgaggact gcacatcata
     3541 ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt tggcatcata
     3601 gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc tcctgcaacg
     3661 atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     3721 ggatgggcta ccatctcaac aatttcacct acaacaaact taggttcctt atcattaaca
     3781 atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac ttgctcatct
     3841 gtcaaaaaga cttgaccgcg ctctgcaatt tcaaataaac ttgaaacttc gaaaatattc
     3901 caagccactg tttccccatt atctttgaga aaaacacggg ctaccttgcc tttgcgctcc
     3961 acatccagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     4021 tctttattat atgtaaaaat cattgtttcc tttttctcct atttcagtcc tgctaaaaag
     4081 tcattgattt gttgcttgct tttacggtcg cgattgacaa aacgaccgat ttccttgtcc
     4141 ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc caaatccata
     4201 tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc aatctctggt
     4261 aaggcaggat aaatataacg acaatcgcta caccagtctg ccacaaaaat gaagaccttc
     4321 ttgcccgctt tttccactaa agatgctaat tcttctaaac ttgctggctg tatcataaga
     4381 cttcctcctc atagactagg tcttcatttt catagacaaa ggtataatga cggccatcct
     4441 caaaaatgac gccaccaacc aagctctcca gactgctttc gtaaacttga acataaaggg
     4501 tcgcaatttc ccccatgtcg gaaaaatggt ctcgcacaat ctctgtcaac tcttcctgag
     4561 tcttcatgag cttacggtca tctgcaactt ttttcgtagc aagagcaagg cttccgatac
     4621 ctagcagagc caagcctgcc atccacattt ttttagcttt cataccattc attttaacac
     4681 aaaaaaggct tcaggacaaa tgaggaagca gcagaaaagc aagtaaaaag cctcttcctt
     4741 taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagga agctagccgc
     4801 aggctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gctcaaaaca
     4861 ctgttttgag gttgtggatg aagctgacgt ggtttgaaga gattttcgaa gagtattatt
     4921 cttattgcca ggcacctaag ttgccaacgt agtaactatc aggtgtgtag gtattgcgaa
     4981 gcatcttacc tgatgaagcc agataatact acttgccatt gtctttgacc caatcattcg
     5041 caatcatgga accagaagaa cttacataat accattctcc cttgtcataa acccaagtac
     5101 tgactttcat ggttcctgag caattaaagg caaaaaaact gtccaatatc attcgttttt
     5161 taaaagcatt tgacactaca tcatctacgc tgtagtacat agatacatca aacgtaaaag
     5221 ccaaaatatc gaaaaatata caacttttaa aggcagcatt tcatttttta aagtctaaca
     5281 accatagaac tgaaatcaga gcaaggcatc aatttcctac tcttagtacg tctattttga
     5341 tggttgaagt gaagattttt ttatttaacg tagacaattt ccaacttccc aaaagccaca
     5401 tctccacgga taatcaaggt tttgctggtt ggggctacag gagcgtcagc cttagctgca
     5461 ccaaaggagg tttctacttt caaatctaca cgccagtgtt gtggaacata gacggttgca
     5521 ttcccgaagg ccacttcaat atttaaagtt gcaaaatcac ctagcatctc tgcattatca
     5581 tagtagattt tagcatcccc aaaagcgact tccacttggt catctacgag atcttgatct
     5641 tgcttataga aggtcccgct accaaaagcg acttccttat ccgtgacgac tgtttttttc
     5701 ccattgtacc accatttttt ttcattccag aacttacttg aatgcgtcag ataaccaaca
     5761 ccaagtacca ccaagatgct agcccaaata agagaatgat tggtaactgg taacaagtca
     5821 taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa aaactgccga agtgagatga
     5881 cgtctaagga tggactcaat ggacttataa gcaaaaaaaa caatacctag taaaggccat
     5941 attttaccat ccaaagaagg aattccaaaa ttcccttgca gcaagatcca agctgctaaa
     6001 accaataaaa caataccaaa tgctttcttt ttcatgtttt ttacctcatg tttcttagat
     6061 tttcttttag gagggattgg taatgccgtg agacatgaac ctccttgtgc gtctgataaa
     6121 aggaaatggt gcccgttcct gaaaaggact tgtccactga gtaaatctga cggatgtttg
     6181 cgatcgttga cttggaaact cgattaaaat agcgaggcaa gatagactcc aactcataga
     6241 gcttgaggcg aacctcataa gcttccttct ggttatgagc gtagatcttg ctcccttctg
     6301 tttcaaagaa gagaatttct gacaggtcta gataatattc acctgtcccc ttgtaaaaag
     6361 tcaacctcgg agcctttgac tcttgcaaga ggcgttgcaa gtctgcaatt tcatctgtca
     6421 aagtgggggt cttgatgaca atttcagtct cctctaaatt gccgtcaatc tcaattcgta
     6481 acttcatcac atctcctttc tgactctact atatcaaagc taagttagga atacaagggc
     6541 aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta ggactagctt aacttacatg
     6601 tcagaatagg aagaaaagga atccccacac caaaccacaa gcatagccaa ccaagacatc
     6661 cttgggataa tgaacacccc ctaaaaccct caccaacccc agcaaagctg aaaagatcaa
     6721 gcatactaag ccaataggta gactagcatg cagacaggcc atggagatga tagttgccga
     6781 aaagacatgg cgactgggca tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat
     6841 atcccaagtt tcataaggtc tcgggtgatt gattttctta cgaaacaggg acaaggccac
     6901 aaaacctgat gcaggaataa gcacataaac cccaacttgc tgaccaagtc ctagttgcag
     6961 gtaagtggtg actagcaaga tcagatagac caggggcatg gcgactgtca tcaaacgatt
     7021 gaaactgcgt aataggaata aaagaattgg acaactagtt agtctggagc tgatatttcg
     7081 ataccattct tgataatttt tcatttatgc ttctcatact tttctagaac ttgttgagat
     7141 gttttcccct tggcaagcca atccactagc ttgtccaagc gacgtatttc ctgcatcaga
     7201 ggatcatcaa tttcctcaat acggacaccg caaatcttcc ctgtaattgc aattcgttca
     7261 gggacataat atggtgcttg tctaaaaaaa tctccgtaag taacatccct atccagacaa
     7321 gcaagaacat cactcacttc ataccctgtc aaccaactgg tcacttggtg aactgactct
     7381 gcctttccac cctttcgctc aacttttgca atcaaggcta agtagacagc agcaaatttc
     7441 atattgtata atttctgact catacttctc tctttctatg tgtactagta taaataagaa
     7501 aaagaaggcc gtcaagcctt cttttgattt attcttctgc ttcatcttct gtaaattgac
     7561 tattgtacaa gtcagcgtag aagccacctt gcgccatcag ttcctcatag ttgccttgct
     7621 cgatgatatt tccatctttc atgaccaaga tcaagtctgc atttcggatg gttgacaagc
     7681 ggtgggcaat gacaaaggat gtgcgtcctt ccatcaaacg gtccatggct ttttggatca
     7741 attcctctgt ccgtgtgtca acagaagaag tcgcctcatc caaaatcaaa agcggtgcat
     7801 ccttaagaag ggcacgagca atagtcaata gttgtttttg tcttacagac aaggtcacgg
     7861 tgtcatccaa gatggtatca tagccatctg gcaaggtcat aataaagtgg tgaattccca
     7921 cagccttact agcttccatc attcgttcat cactaatccc tatttgatta tagatgagat
     7981 tgtctcgaat agttccttca aagagccagg tatcctgcaa gaccattgaa aaggcatcat
     8041 gcacttctga acgcgtcata gccttggtat ccacaccatc aatgcgaata cttcccttat
     8101 caatctcata gaatttcatc aaaagattga caatggttgt cttaccagcc ccagtcggcc
     8161 caacaatggc aaccttttga ccagcatgag ctgtcgcaga gaagtcatag tcttgaacat
     8221 tgacaccgtc caccagaatt tctcctgctg acacgtcgta gaaacgtgga atcagattga
     8281 ccagagttga tttaccagaa cctgttgacc caataaaggc cactgtttga ccagtttctg
     8341 ctttaaagct aacatgttca ataactgcct ccgaatttgc cgcatagcgg aaggtcacat
     8401 ccttaaactc gacctgacct ttgaagtttt catcagtcag ctgcacttga acagggtttt
     8461 ggatagaaga atgcaaatct aaaacttgat taatccgctt agcagagacc atagttcggg
     8521 gaagaacgat gaagagtgct cccatgagaa ggaagcccat gacaacctac atggcataag
     8581 acatgaaaac aatcatgtca ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa
     8641 tcacataggc cccaatccag taaatcgcca cactcaaacc acttgaaatc cccatcatga
     8701 taggattcaa aatagccata agacggttga caaacaaatt caaacgggtc aattcatcat
     8761 ttactgctgc aaatttttca ttttgataat cctctgcatt gtaggcacga acgacacgaa
     8821 tacctgttaa actctcacga gtgatactgt tcagtttatc tgtcagcccc tgaatcaagg
     8881 actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat caggacgttg ataatcactg
     8941 ccacaagtac ggcccagagc cagtattctg aatgacctaa aatcttccca atagcccaga
     9001 tagccataat tgaaccacgc gttaccactt gcaagcccat agtaatcaac atttgaactt
     9061 gagtaatgtc attggtagta cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg
     9121 tctgcgagta atccaaaact cggttaaaaa tatcacttct cagcctacta gtataagaag
     9181 ccgccactcg ggatgcaaaa aatccaactg caactacgga caagaaggca agaaaggaca
     9241 ttcccatcat catgcttgcc gactgccaca actcatctaa attagtttct tgactaccta
     9301 gcaaatccgt aattttcgag atataggtcg gcacttccaa ctctagatag accgaaaagc
     9361 aagtaaagag aatggctagt aaaatcatcc cccattcttt tctactaatt cttttggcta
     9421 atttctttat tctctcctcc tattcccttg atattttgcc tgtagttgac cgagaacctt
     9481 ctcaaaaatc agtaattcat cttcatcaat gtcttccatc aactgcttgt ctatgcgttc
     9541 aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg tccgtcagac gaacaaactt
     9601 agcccgctta tcaacaggac tcgcctccaa ttccaccaaa ccattttgca ctatacgctt
     9661 aaccagatta ctagcaacag gcttggtaat attgagttcc tgctcgatat ctttaatcaa
     9721 gaccaagtct tggtttttct cgcgattatc caaaaaacgc acaacctgac cttgcggccc
     9781 acccataaat tcaatgccgc aacgtttggc ttccttttgc accatcaggt gaatttgatg
     9841 accaaaacgc ttaaagacta acatcggttt atccataatc tcccccttct aaataaaaat
     9901 agttctctgg agaataatta aatttctatg agaactattt tcttgattaa aaaaatccca
     9961 agtgattttc tcacttagga tcatgttcta taggttaaat taaaacccat ctacgttcgt
    10021 ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg taaagttttt caaaggtttc
    10081 aaggtcttcg cctgacaatt ccacttctga ctgaggaatc atttccaatt cagtcacttg
    10141 gaattcttca ataccagact cacggagggc aacgatagcc ttgtgaaggt cagttggcgc
    10201 tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca tccacatcca catccgcttc
    10261 gagcaattgc tcaaagactg cgtccgcatc ttcacctcca aatacaataa cacctttgtt
    10321 gtcaaagagg taagaaacag aacctgaagc gcccatgttt ccgccgtttt taccaaaggc
    10381 tgcacggaca ttggctgctg tacggttgac gttagaagtc aaagtatcca caattagcat
    10441 agagccattt ggcccaaaac cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc
    10501 tttggcttta tcaatcgctt tatcgataat gtgttttggc acttgggctt gtttagcacg
    10561 gtcgataacg aatttcaaag ctgagtttga ttctggatct ggatcacctt ttttagctgc
    10621 tacatagatt tctacaccaa attttgcata tactttagag ttagctccat ctttagccgt
    10681 tttcttggct acgatattgg cccatttacg tcccattagg aatctccttt tttcacattt
    10741 taatctttct tattataaca caagtttttt tgattttcac tagaggaaat ggattttatt
    10801 agcaaatcaa gctaggatag cactttacct gctaagatgg tcttgccttt ctatctttat
    10861 caacaggcac tcatccacat tcaaaaaaca aactagacca ttatctgcaa atagaaagtt
    10921 tcagccaagt ttgacaaagt cagctcaaat tactgtttga agtttgtaga tataagcgac
    10981 aaaaacaatc atactgcacc ttttgttgac agtctactcc agacatatca tagttcaagt
    11041 aaatactttg aaattcaaca gttcttatag gcgctattgt attctaagaa atcaatagaa
    11101 gagtttctaa gcaaacctct aatactcaat aaaaatcaaa gagcaaacta gaaagctagc
    11161 ctcaggttgc tcaaaacact gttttgaggt tgcggatggg gctgacatgg tttgaagaga
    11221 ttttcgaaga gtataattta cgtgttccca agatggagaa gttagactag tacactggca
    11281 cttctaaaac attgctagca attgatttgt tcatatttaa tttcattttt tccataaatg
    11341 ggtattagat ataaacagca aaatatttcc gatacgtgtc gttcttgaat ttccaatcat
    11401 ctaaaacaag taaaggataa tcaatcccct gtatatcaag gaattggcta ccctttttac
    11461 ttttttacac attctgtttg atagattcat tttaacatca cgagcatact ccaatggaaa
    11521 tcgctaggca agagataaac tttcagatat ccgcagagag atcatcgcct ctttttgtcg
    11581 caagcattct cctctcctag tcattttcta ccttatcttc tacctgagga tagagagttg
    11641 ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa atcggttttt tcataaaccg
    11701 tacgccacca ttcccaggca agcccggtac actctctaat tttgacagag agattacgaa
    11761 cattcccttt taaaggaata ctagtggtaa agtgagccgt taaatcctgc ccatttctgt
    11821 cccaagcctt aggagtcaag acttccttac cttgatgatc ataggataat tcattccaag
    11881 taatataata ttgggcaaca taggcaccac tatgatccag cagtaaatct ccgtttctgt
    11941 aagctgtaac cttagtctca acatagtctg tactattttg aaaggtcgca actacattgt
    12001 cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg atgatctgct gtaaagcgac
    12061 tgccttcttg aatcaagtcc tctaccatat ccaccttgcc tgttacaact cgggcacccg
    12121 aacttgggtc gccccctaaa ataaccgcct tcacttctgt attgtccaaa atctgtttcc
    12181 actctgtctg aggagctacc ttgactcctt ttatcaaagc ttcaaaagca gcctctactt
    12241 catcactctt actcgtggtt tccaacttga gatagacttg gcgcccataa gcaacactcg
    12301 aaatatagac caaaggacgc tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta
    12361 cagtatcttg aaacacatct cctggatttt taacagcatc tacgctgact gtataataaa
    12421 tctgcttaaa attaacaatc tgaatctgct tttcgcctga atggacagag ttaaaatcaa
    12481 tatcaagaga attccctgtc ttttcaaagt cagaaccaaa cttgaccttg agttgttcca
    12541 tgctgtgagc cgtgattttt tcatactgca ttctagctgg gacattattg acctgaccat
    12601 aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg
    12661 ggtcttccac ttggagaaag ctatcgctac ttgccaaacc aggcaaatca atactataag
    12721 tcatcggagc acgatcgacc gcaagaagag tgggattatt ctctaacaag gtctcatcca
    12781 ctacgagaag tgctccagga tagaggcgac tgtcgttggt agctgttaca gaaatatcac
    12841 ttgtatttgt cgacaagctc cgcttctttc tttcgataac aacaaactca tcgggtagct
    12901 gattaccctc tttgatgaaa cgattttcaa tactttctcc ctgatgggtc aagagtttct
    12961 ttttatcgta attcatagct agtataaagt catttactgc tttatttgcc atcttctacc
    13021 tcctaataag ttcctggatt gagttgcata aactcagact tgttcagcga aatcagccgt
    13081 ggttggacta agtaatccaa aatttcctcg tacaattctt ctgagacatt gcgtcgccgt
    13141 ctggctaaat aagaagtcgg aatgaccgta ttatccaaca taaatacctt atctaagtca
    13201 atcaaggttg gtcttgtaaa aggattacga gctagatccg gctcttctat cataaagttc
    13261 ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct gatttttaac caactctttg
    13321 tttttagtca tgctgttgtc aatacagata tacatatgat tcttcacagc caaatcgcta
    13381 ctaatagtcg gaaaaggcaa ataaagagct acaacatctc ctctcttaat caagcaagag
    13441 cacccccttt tctcctaatg taacatagac aggattgacc aagtcttctg attgactcag
    13501 aatttccaaa gtttgagttt ggcgcgctgt caatttagta gcatcttgtc tcttcaatac
    13561 aaaatgcttg tcgccaataa ccttgacaat ataatccttc tccaaagctg actggtaaat
    13621 ccacatcaga tgttgtctgt cctgagaact caagagagaa ggattttcaa gcctcccgat
    13681 agtctgataa aaatcaaaaa caggagctaa ctcctgccaa tctgattggc tagttgtcaa
    13741 ggctagaaaa agggctttgc gagctgatac ttcttggtta gccttgagag ttactttccc
    13801 ctccaagttt tttagaaatc gggaaactcc agaaagcaaa tttttctcta actgcgagaa
    13861 ataaaaacct ttcgttccca gacataagtc tttcatgtcg ctttctctag caaataagag
    13921 ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg gtcgcgctca tcttttcctt
    13981 atcggcttct ttttttaacc agagcaaggg cgacaggtag ctggattgag acatttcctc
    14041 tacctcctac tcttttttaa ctggagcatc tgcactagct gccacttctt ttgactggat
    14101 actttcccac tggttaatct cctctgagat aagaccttcg catgtcttga caaatagggc
    14161 aaaagccttg gtctttcctg catatttctc cgtttggcat tgatagagga atttttcttt
    14221 ctccaggagt tgcgcagttt tttggtaaga aatccaattt tcctttgcat tatacaaatt
    14281 gataatcccc tcacacagca agccgagact ggataaggca accgaaatca aacggtagcg
    14341 atcacctggc ataggaatag cacaaaagac agctatgagg aaacctgcca cgatttctgt
    14401 tatttttaat accttatagc gcctacgatg ttgaacgctt ttctttaaaa aatgagctat
    14461 ctgtacgtct aatcgctctg tcaggtacat ttcttctggc gtcatattcg taactccttt
    14521 catttacttt gataatcata gtataacata tttgaatatc aaagtatagt aaaaactgga
    14581 ctataactta ttagacttcc tgcgaaacaa aatatggtat agtagttcta tgaattatga
    14641 agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc agcgcacgac
    14701 ttttgaagag atattagctg tattaaaaac agcttatcaa cttaaacacg caaaaggtgg
    14761 acgaaaacct aaattaaacc tagaagacct tcttatggcc actcttcaat atgtgcgaga
    14821 atatcgaact tatgaacaaa ttgcggctga ttttggtatt cacgaaagca acttaatccg
    14881 tcggagccaa tgggttgaag taactcttgt tcaaagtggt gttacgattt caagaactcc
    14941 tctcagttct gaggacacgg taatgattga tgcgacggaa gtacaaatca atcgccctaa
    15001 aaaagaatta gcgaattatt ctggtaaaaa gaaatgccac gctatgaagg ctcaagcgat
    15061 tgtcacaagt cgagggagat tgtttctttg gatatcactg tgaactattg tcatgatatg
    15121 aagttgttca aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt ggctgacagt
    15181 ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa
    15241 ctcaagccgc taacagctga agataaagcc tataaccatg cgctatctaa ggagagaagc
    15301 aaggttgaga acatctttgc caaagtagaa acgtttaaaa tgttttcaac aacctatcga
    15361 aatcatcgta aacgctttgg attacgaatg aatttgattg ctggtatttt caatcatgaa
    15421 ctaggattct agttttgcag gaagtctatt atatatgcac agacatatga aatctttttg
    15481 agatagcagt tatgatatat ttatccattc acaacatctt ctgtaccaat actacaatat
    15541 tggtacattt tgattacaca atcgtttcaa aaaatatgat ggaagcctga gtattttata
    15601 gtaactccct tcctatcaac tagatcaacc agttgagtgt tgaatgttga aacaccctag
    15661 cctcagcaaa ggactatgag cagcatttcc tagctaatag gacgctccaa atcacctatt
    15721 ctaaatccat cactagcttg gtcagtaaga tacagactgc aaggcaagac tgacgtggtt
    15781 gatgagattt tggaagagta ttacaatcat agattcacca gtagacttga ccgcagattt
    15841 aactatagta gattgaaact agaatagtac acttctgctt ctaaaacatt gttagaaatc
    15901 gatttgactg tcctgatcaa tttgtcctgt tctcgtttca ttctactata ctaaaaaacc
    15961 acctgaaaca aatcaagtga ctagaggata aaaacgaaga ctgggcaaaa agttttgaaa
    16021 agcaaaaaac gcataatatc aggtcttgaa aaaccttgat actatacgtt tgattgtggg
    16081 aagattgact tcattttctc ctgaaattga agttttttct agtttccaga tatagacgaa
    16141 ttcaatatag ccatctcgct acaattattt taaccatcga gaaaattttt cagaatttca
    16201 aaaatttcat tttgatccaa tgaaatgtca ttattttgaa gaatagttga cttcacaggc
    16261 tctaattcta tcacaaagcg gtgcatgtct gcttttaccg cactacttga tgttatagcc
    16321 gataactttt catctcctag caaggaagct gtcaaacggg taaggtcatt caaatgcttt
    16381 ttaatggact tacttaaacc ttgagcacct gtggcagagc gctccctcat atccagccaa
    16441 gctttagcct taaaaataat taatactcaa tgaaaatcaa agagcaaact aggaagctag
    16501 ctgcaggctg ctcaaaacac tgttttgagg ttgtagatgg aagctgacgt ggtttgaaga
    16561 gattttcgaa gagtataaac cacaattact caataccgaa tacccctgaa tggtttcttt
    16621 ttcatgcacc aatatattat aataatcttc atccaataat aaggctgata aactagcatc
    16681 ttggtcaaaa tgtaagggaa tttctcgacc gtccttcttt aatggatatt ctggtaagat
    16741 actaaatagt tcaatcatag aaggaaactc aggattagtt gttgtaaatc gaaaaagctg
    16801 cgctttctca tctttctgac ttccttgata ctctcccaat tctaaaaaat gattcaaggt
    16861 agtataaaat tccttatttt ttacttcatc aatgatgacc atatcataat cttttgttgt
    16921 gcgactttta aatccttgcg aatccaatac gatagaggta gcagttcccc caatcagaac
    16981 ataatagttc tgaaaatccg caaacatttc ttgaaaaata actttcgtat tagctggcat
    17041 catcttctcc cagatactgt aatatcatat tttctagtgc ttcactctct tcctctatac
    17101 gtgggtcatc atcatctttt aaggtcaaat aaagagaaat cggatctaca aattgtttat
    17161 catgattatt tttaaaatca ttccaaaact cagatacaaa aggacgatat ttccatatct
    17221 ctagcatctt tcattttaaa acatgctgag aaagtggcaa ggataactga ttgaattttc
    17281 tctgccatat gacatagcta atattttcat ccgtttcagc taaaaaagtt gaatgcgaca
    17341 aagcataagc accaccatat agaaggttag aaacagattt tatctgcttt atatcgccat
    17401 ctggcaataa aatccgtttt ttgatgggat taaataaaca tgacacggat tttaagaata
    17461 attctttctt tgacaccgta tatgtgtaaa gcttattttg cttgtttaac caatataaag
    17521 ctttaaaagt cctcaaacac ctataaattg ttgagtttgg aagtccagtg acttgtgaaa
    17581 gcaaatcaac atctactact ttttgacctt ttgtcaataa aaaggcaatc cacgttaatt
    17641 gttcgctagg tgttaattcc ttagggactt cagtatcatt cgcattgagt actagtccca
    17701 atggagggaa gaagaggttt cccttaaagt ctacaaacgg aactctagct tgaagtaatt
    17761 gctttttttc actgtctgat aacttcgaaa acaccaaaac aacatccata ttggcttttt
    17821 cacccatagt gcgagcctga gtaacaaatg aactcaaact cccccttctc ttttctttaa
    17881 tcaataaaaa tgattcctta tgaaaagtca tttgagtata ctcaaacctt tttacaaaac
    17941 tgattggtaa agtcaaagtc agttcctcaa tactgacatc aacataaaag gattgaaaaa
    18001 ttgacattat ctttttgaca ttcatgtttt ttccttattc tatcattttt attatatttt
    18061 atcattcggt at
//
LOCUS       DAVHEW010000037        17805 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVHEW010000037 DAVHEW010000000
VERSION     DAVHEW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.037"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            281..1327
                     /gene="pheS"
                     /locus_tag="VVL53_001128"
     CDS             281..1327
                     /gene="pheS"
                     /locus_tag="VVL53_001128"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU4183312.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1327..1836
                     /locus_tag="VVL53_001129"
     CDS             1327..1836
                     /locus_tag="VVL53_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183313.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1913..4318
                     /locus_tag="VVL53_001130"
     CDS             1913..4318
                     /locus_tag="VVL53_001130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU4183314.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILLEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVIIEPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPREELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4386..5387)
                     /locus_tag="VVL53_001131"
     CDS             complement(4386..5387)
                     /locus_tag="VVL53_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4183315.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5543..5958
                     /locus_tag="VVL53_001132"
                     /pseudo
     CDS             5543..5958
                     /locus_tag="VVL53_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6084..6350
                     /locus_tag="VVL53_001133"
     CDS             6084..6350
                     /locus_tag="VVL53_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183316.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6611..8860
                     /gene="metE"
                     /locus_tag="VVL53_001134"
     CDS             6611..8860
                     /gene="metE"
                     /locus_tag="VVL53_001134"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU4183317.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSTCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEANRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8924..9790
                     /gene="metF"
                     /locus_tag="VVL53_001135"
     CDS             8924..9790
                     /gene="metF"
                     /locus_tag="VVL53_001135"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU4183318.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10475..12688
                     /gene="pnp"
                     /locus_tag="VVL53_001136"
     CDS             10475..12688
                     /gene="pnp"
                     /locus_tag="VVL53_001136"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU4183319.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            12704..13321
                     /gene="cysE"
                     /locus_tag="VVL53_001137"
     CDS             12704..13321
                     /gene="cysE"
                     /locus_tag="VVL53_001137"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU4183320.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13333..14217
                     /locus_tag="VVL53_001138"
     CDS             13333..14217
                     /locus_tag="VVL53_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183321.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14299..15642
                     /locus_tag="VVL53_001139"
     CDS             14299..15642
                     /locus_tag="VVL53_001139"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU4183322.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15635..16021
                     /locus_tag="VVL53_001140"
     CDS             15635..16021
                     /locus_tag="VVL53_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU4183323.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16025..16909
                     /locus_tag="VVL53_001141"
     CDS             16025..16909
                     /locus_tag="VVL53_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU4183324.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17045..17500
                     /locus_tag="VVL53_001142"
     CDS             17045..17500
                     /locus_tag="VVL53_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183325.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            17497..17643
                     /locus_tag="VVL53_001143"
     CDS             17497..17643
                     /locus_tag="VVL53_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183326.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
BASE COUNT         5344 a         3440 c         4035 g         4986 t
ORIGIN      
        1 agaagtaaga taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat
       61 aggaatctga aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca
      121 agctctctat atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg
      181 acttgtcaga tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat
      241 gtggttttct ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca
      301 attaaaagcg cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga
      361 aaaagagatg caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat
      421 cctcaaaggg atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa
      481 tgaagctcgt gatgtcttga ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa
      541 gaaagtcgcg gctcaactgg ctagcgagag tatagatgtg acgcttccag gtcgtccagt
      601 tgcgactggt caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat
      661 cggtatgggt tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga
      721 acgtatgaac cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac
      781 tgaagaaatc ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca
      841 tgatttttct aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac
      901 ggacgatgcg acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa
      961 tatctctatg gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga
     1021 agagcgtcaa attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt
     1081 ggatgtttct tgcttcaagt gtggtggaga aggctgtaat gtatgtaaga aaacaggttg
     1141 gatcgaaatt atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga
     1201 tgcgactgta tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg
     1261 ttatggaatc aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt
     1321 taaataatga ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa
     1381 caagcctttc gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt
     1441 gaagaggctt atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc
     1501 tatttcatta tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct
     1561 caaactgaga gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa
     1621 ttccaaggat ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa
     1681 aatagttttt cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt
     1741 tataaccgat atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta
     1801 gatacggatt ggttactgag aaagaaatta aggtaagaag agaattcttc tattgaaatg
     1861 ataggaaagg aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttgt
     1921 atcttataaa tggttaaaag aattggtgga cattgatgtg ccatcacaag agttggctga
     1981 aaaaatgtca actacaggga tcgaggtaga gggtgtcgaa tcaccagctg ctggtctctc
     2041 aaaaattgtc gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc acctccatgt
     2101 ttgtcagatt aacgttggcg aagaagaaga gcgtcagatc gtttgtggtg ccccaaatgt
     2161 gcgtgctggg atcaaggtca tggtggctct tccaggagct cgtatcgctg ataactacaa
     2221 aatcaaaaaa ggaaaaatcc gtggtttgga gtcacttgga atgatctgtt cacttggtga
     2281 attgggaatt tctgactcag ttgtgcctaa ggaattcgca gatggcatcc aaatcttgct
     2341 tgaagatgcc gtgccaggtg aggaagtctt ttcttaccta gacttggatg atgaaatcat
     2401 cgaactttcc atcacaccaa accgtgcaga tgccctttct atgtgtggag tggctcacga
     2461 agtggcagcc atctatgaca aggcagtcaa ctttaaagaa tttactctaa cagaaactaa
     2521 tgaagctgcg gcagatgccc tttctgtcag cattgggaca gacaaggcgc cttactatgc
     2581 agctcgtatc ttggacaatg tgattatcga accaagtcca caatggttgc aaaaccttct
     2641 catgaacgaa ggcatccgtc ccatcaataa cgttgtagac gtgacaaact acatcctgct
     2701 ctactttggt caacctatgc atgcttttga cttggacaca tttgaaggga ctgacatccg
     2761 tgtgcgtgaa gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt
     2821 ggacgtgaat gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat
     2881 gggtggtcaa gcaacagaaa tctctgaaaa atctagtcgt gttgtccttg aagctgctgt
     2941 tttcaatggt aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt cagaatcgtc
     3001 ttctcgcttt gaaaaaggca tcaatgtggc aacagtcaat gaagcccttg atgcggcagc
     3061 tagcatgatt gcagaacttg caggtgcgac ggtgcgtaag ggtatcgttt cagcgggtga
     3121 gcttgatacc tctgatgtgg aagtttcttc aacccttgct gatgttaacc gtgtcctcgg
     3181 aactgagctg tcttatgctg atgtagaaga cgtcttccgt cgtcttggtt ttggtctttc
     3241 tggaaatgca gacagcttta cagtcagcgt accacgtcgt cgttgggata tcacaatcga
     3301 agctgacctc tttgaagaaa tcgctcgtat ctatggatat gaccgcttgc caactagtct
     3361 accaaaagac gatggtacag ctggtgaatt gactgtgaca caaaaactcc gccgtcaagt
     3421 tcgtaccatt gctgaaggag caggtttgac agaaatcatc acctatgctc taacaactcc
     3481 tgaaaaagca gttgagttta cggctcaacc aagtaacctt acggaactca tgtggccaat
     3541 gactgtggat cgttcagtcc tccgtcaaaa tatgatttca ggtatccttg ataccgttgc
     3601 ctacaacgtg gctcgtaaga ataaaaactt ggccctttac gagattggaa aagtctttga
     3661 acaaacaggt aatccaagag aagaacttcc aaatgaaatt aacagttttg cctttgcctt
     3721 gacaggcttg gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta
     3781 tgctaaggga atccttgaag ccctatttac tcgcttggga ctccaagtaa cctatacagc
     3841 aacatctgaa atcgctagcc ttcatccagg tcgtacagca gtcatttcac tcggtgacca
     3901 agttcttggt ttccttggcc aagtgcatcc agtcactgct aaggcttatg atattccaga
     3961 aacgtatgta gctgagctta acctttcagc tatcgaagct gcgcttcagc cagcgactcc
     4021 atttgtagaa atcaccaaat tcccggcagt cagccgtgac gttgcccttc tcctcaaggc
     4081 agaagtgact catcaagaag ttgtagatgc tatccaagct gccggcgtga aacgtttgac
     4141 agatatcaaa ctctttgacg tcttctcagg tgaaaaactg ggacttggta tgaagtcaat
     4201 ggcttatagc ttgaccttcc aaaatccaga agacagctta acggacgaag aagtcgcacg
     4261 ctatatggaa aaaatccaag cgtctctcga agaaaaagtc aatgcagaag tgcgttaaac
     4321 gatagtagga tacaaattcc ccctctagtt tatgaacatt agaggggtga ttttatataa
     4381 aataattaaa atgtgatttc tgtaaaaata catctatgat cagagttttg aaaacttaca
     4441 atgtctaaat ctttaacata gtagtggttt ttaggcaata aaatatgatc tattgttgca
     4501 ttaaaaagtt ttggactttg gctattccaa gttcctcttt caaaaggtgg cagtgcattt
     4561 aatgcgtccc tatgagagct tatttttgca agtgctccat gacgcatagt tgcattaaaa
     4621 tcacctgcaa taatagcctt tggatatttt gaagccaatt gattatgaat gatgtttaag
     4681 tcttgcttcc agatttccat taaacctggc agaggaggag cagtatgcaa ggcaatgata
     4741 tctggtatat tttgttttct cgaatgtaat acaattgtcc cgaaccgtgt tgtatgaaaa
     4801 gttttagctt ctgtatagaa accataactt ttcttgacaa tcacagtcac aggagctatt
     4861 ccactattgg taggtggaga agtgaaaata tcatagttgg ccatagaaag tccaacttga
     4921 tgaaacaata gtttgattct ctggttttct tgctcacctc tgatattggt agctagttca
     4981 gggaatatag ccatatcggc gtcaaaatgg ctaaaaattc gctcaatatg ttgtgcttct
     5041 atttgattag cgacgttcca agttactaat tttaaggttt tagtcttatt tgatgcagaa
     5101 gaaagggttt tatctgttcc gaataatagt aatatgagcg atagtaagag caaaatgcca
     5161 gagtaaaacc gtatttgttt atttttcttg taaaaagata ggaaaaaaaa taagaaactt
     5221 aaaatagcag ccatagttgc agtagctaag gggaaggcat aaatctgtgc caagataggt
     5281 tgggtagata gccaaatgtt aagattagaa gatgggaaaa aagaataagt tatgataggg
     5341 atagaaaaga gaatgaccga aagggcagag agaaatgttt taatcattgt gtgagtacct
     5401 caatttgcaa tggttgctcg gagctaatgt gactttgtga ctttacttga ttgatattct
     5461 ttttcttgtc ttcctcatag attaagtaca acccaagtaa aaaaagtcca atttaagatt
     5521 gaaatgatta aaaggcacag aaatgcaaga caattatact acaaaaggca aacatttgac
     5581 aatcgatagc cgtcgcttaa tcgaacgatg gaaaaaagaa ggaaaatcaa atagagaaat
     5641 tgcctctcta cttggaaaag ctcctcaaac tatccacact gaaatcaagc gtgggacagt
     5701 ccgacaatgt cttggaaaag ggcgcttcaa agagatttat tctgccgact acgctcaaca
     5761 gtcttaagaa actaaaggaa aagattctcc actatacata accaaaaaca taaccaaaaa
     5821 ttttcgcctg aaatgatggt gatggctaaa ggggttaacg tgggaatttc aaccatttac
     5881 tattggattc atcgtggaaa attggggtta agcaagcagg aactgcttta tcctagaaaa
     5941 ggaaaatcgc ttaagaaata ggctagcacc aactttaaac ccactggtca atctatcgaa
     6001 tcataattcg ctcatttttt taaaaataca tagacgtaaa aaaaagaggg ttaagtgatg
     6061 aagaatagtg ctattgggag taattggaag gatgtccatc tgagctcttt agcaaggagg
     6121 aaatccttga aagtgatatg cgagtggcta tcatgagcga gttgattgag gctaggtatg
     6181 aacaaggaat cagtcagaaa aagctggaag aagtcagtgg aataagccag cctgtcatag
     6241 ctaggatgga gacaggaaag actagtcctc agttggatac agtcttaaaa gtcctagcca
     6301 gtttaggaaa gacactagca atcgtcccac ttgaacaggg aaaaagttga ctagaatcag
     6361 ttatagtctt ttctaataac aggtcatagt cacttataag aattactaat aacatgtttt
     6421 agtattctaa ttggaatata gctgtaaagt aagaattaca aaggatttga gacgtttgct
     6481 tcaagtcttt ttcttttgtc cgaaacagat atatagtttc aaactatatc aaaccctaat
     6541 tttttacaat tatacagacg ctttcttttc tgttaagata gtttcaacaa caaattttgg
     6601 aggacacatc atgtcaacta caatcatcgg tttccctcgt ttgggcgaat tccgtgaatt
     6661 aaaatttaca actgaaaaat actttagaaa agaaatctca gaagaagaac ttttagcagc
     6721 agcaaaagac ttgcgtgcta aacactggaa cattgtcaaa gaaaaaggca tcactgaaat
     6781 tccatcaaat gacttttctc actatgataa cttcctagat gcggctttcc ttttcaacgt
     6841 ggtgcctgca tccgttcaaa acttggactt gtctgacctt gagcgctact tcgctttggg
     6901 acgtggttac cagggagaaa aaggggacgt tcgcgccctt ccaatgaaga aatggttcaa
     6961 cactaactac cactacatcg ttcctaaatt tgaaaaagac actcaagtaa aattggctgg
     7021 tcacaagatt ttcgatgagt tccaagaagc caaagaactt ggattgaaca ctcgtcctgt
     7081 tcttgtaggt cccttcactt tccttcaatt gtcagacttt gaagaaggcg tgaaagcaga
     7141 cgacttcgta gatagcttag tggctgctta ccaagaagtt tttgctaaat tggctgaact
     7201 tggtgcgact cgcatccaat tggatgaagc ggctcttgta aaagacttga cagctgaaga
     7261 aaaagctctc ttcttgaacc tttacaacaa actcttggct gacaaaaaag gtcttgaagt
     7321 cttgcttcaa acttatttcg gtgatgttcg tgacgtatac gctgaccttg taaacttacc
     7381 agtagatgct atcggtcttg actttgttga aggtaagaaa actcttgaac tcgttaaagg
     7441 tggcttccca gctgacaaga ctctctatgt aggtattgtc aatggtaaaa acatctggcg
     7501 caacaactac gaaaagagct tggctgttct tgagcaaatt ccagctgaaa acatcgtttt
     7561 gacaagcact tgctcacttc ttcatgtgcc atttacaact gctaatgaag aatttgaacc
     7621 agcactcttg aaccactttg cctttgcagt tgaaaaattg gatgaaatcc gtgacttgga
     7681 tgctatccga aatggtcaag gttcagaagc tcttgcagcc aacaaagaac tctttgcgac
     7741 tgagcgtgtt ggtgaaaatg ctgaacttcg tgcgcgtatc gctggcttga cagacgcaga
     7801 ctacactcgt ttgccagcct ttgcagaacg tgaagctatc caagaagaag ctttcaaact
     7861 tccagctctt ccaacaacaa cgattggttc attccctcaa acaaaagaag ttcgtgccaa
     7921 acgtttggct taccgtaaag gtgaattgtc tcaaaaagag tacgacgctt tccttgctga
     7981 aacgattgac gaatggatca agtggcaaga agatattgac tttgatgtcc ttgttcacgg
     8041 tgaatttgag cgtaatgaca tggttgagta cttcggtcaa aacttgtcag gatacctctt
     8101 ctctaaaaat ggatgggtac aatcatacgg tatgcgtggg gtaaaaccac caatcatctg
     8161 gggtgatgtc actcgtctta accctatcac tgttaaatgg tctagctatg cacaaagtcg
     8221 tacaaacaaa cctgttaaag gtatgttgac tggacctgtt accatcctta actggtcatt
     8281 cccacgtgaa gacatctcta tcaaggattc aactctccaa atcgcccttg ctatcaagga
     8341 tgaagtactt gaccttgaag ctgctggtgt gaaaatcatc caaatcgacg aggctgctct
     8401 tcgtgaaaaa ttgccactcc gccgtagcga ctggtacgaa gactaccttg actgggctat
     8461 tcctgccttc cgcttggtac actcaacagt agcgccagac acacaaatcc acactcacat
     8521 gtgttactca gaatttacag atatcatccc agctatcgat aacatggatg cagacgttat
     8581 ttcctttgaa gctaaccgtt caaaccttga aatcttggac gaactcaaag cgaaaaactt
     8641 ccaaacagaa gtgggacctg gggtttacga tatccactca cctcgtgtac caaatgaagg
     8701 cgaaatcgac aacacaatcg aagccatcct tgctaaagtg ccaagcaaga aagtttggat
     8761 caaccctgac tgtggtttga aaacacgtgg tatcccagaa acaaaagaaa gcttgatccg
     8821 ccttgttgaa gcagctaaag ctgcgcgtga gaaattgtaa gattggattt ctctccatac
     8881 agtggttgtt caaaaagaaa tcaactagaa aaggttatta catatgtcac gccaaacacc
     8941 gtcactctca tttgaagtgt tccctccaaa cccagccgtg ggtaatgata acattatttc
     9001 agccttgcag gatatgcagg agttggctcc ccattttatc agtgtaactg ccagcaataa
     9061 taaatttaat atcaaggaaa cgacggttcg tttggctgac tttatccaaa atgatttggc
     9121 gattccgacc attgcccact tgccagctat ttatctaact aaggacaagg ttgctgaaac
     9181 tattgctgac ttggacaaag ttggggtgca gaaaatcttg gctcttcgtg gggatattat
     9241 tccagacgtg gaaccacaaa aggatttccg ctatgcaacc gacttgattg agtttatcaa
     9301 ggaacaaacc cctcactttg atattattgg agcttgctat ccagaagggc atccagattc
     9361 gccaaatcag atttcagata ttcaaaatct taagaagaaa gtggatgcag gctgttcgag
     9421 cctcgtaact cagctcttct ttgacaatga gcgcttctat gattttcagg acaagtgtat
     9481 cttggctggg attgatgttc ccattcatgc aggaattatg ccaattctga atcgaaatca
     9541 ggctctccga ctcttgaaga cttgtgagaa tatccatctt ccacgcaaat ttaaagccat
     9601 cttagacaag tatgagcatg accctgagtc gctcagagca gcaggacttg cctatgcagt
     9661 ggaccaaatc gtggacttgg taactcagga tgttgccggt gtgcatctct atacgatgaa
     9721 taatgctgat acagcaaaat acatccatca agcaacccat gccttgttta atcaccagtc
     9781 tctaggataa taaaagcaaa ccattcttct caggtgaggg gaatggttcc tttttaatgg
     9841 taaagacctc actttttaag aaaaatatga taaaataaat tctgtacgta cttgatacaa
     9901 agatgaaggt attaaataat acaatgcatt taaactgtaa gatttaacga cacgagtgag
     9961 tcaaatcgat gttatttgac gagagagtta gtaaagcatt tagctaatcg cccgatagcg
    10021 attagcgtga aagctttaga tactttagta ttaaaccttt ctaatgatgt atcttgaaaa
    10081 ctgaacttgc ctacaactct gtggaaaaag ataaatctcc ctaggagtag tcgctccgtc
    10141 gttcgatttc ctatcttcct ttgagttgct tgacggcttg gtatcttgat gacttgaatt
    10201 ttggtctcat ttcttaggaa aaaagataag cttcctagca ctcgtcgagt gcgacgtcac
    10261 cttcctattt ttctacagaa atgttcggca agccgaaccg tccaaaatat cttgtgtaat
    10321 tgaacacggc cgaaaagctg tgtaaaaaag ataaactgtc ttgtcttcat cgaagacttc
    10381 gtcagtttcc tatttttact ttgcttttga cgtccttggt atcttgatct ttgtaggcaa
    10441 ggcgtataat ttcatcaatc caaaggggat taaaatggca aaacaagtgt ttcaaacgac
    10501 ttttgcgggt cgtgagttaa ttgtagagac tggtcaggtt gctaagcaag caaatggatc
    10561 tgttgtcgta cgttacggtg agtcaactgt cttgactgct gccgttatgt ctaagaaaat
    10621 ggcaactggg gatttcttcc cactccaagt caactacgaa gaaaaaatgt atgcggctgg
    10681 gaagtttcct ggtggcttta tgaaacgtga aggacgtcct tcaacagatg cgaccttgac
    10741 agcgcgtttg attgaccgtc cgattcgtcc tatgtttgcg gaaggtttcc gtaatgaagt
    10801 ccaagtcatc aatacagtgc tttcttatga tgaaaatgca tctgcaccaa tggctgctat
    10861 gtttggttca tctttggcac tgtctatttc agatattcca tttgacggac caattgctgg
    10921 ggtacaagtg ggatatgtag atggccaaat catcatcaac ccaagtcaag aacaagcaga
    10981 gcaatctctt cttgaattga cagtagctgg aaccaagcac gctatcaaca tggtagagtc
    11041 tggtgccaaa gaattgtcag aagaaatcat gttggaagcg ctccttaaag ggcacgaagc
    11101 tgtcaaagaa ttgattgcct tccaagaaga aatcgttgct gctgtcggta aagaaaaagc
    11161 agaagtggaa ttgcttcacg tggatgctga attgcaagct gaaatcattg cagcctacaa
    11221 cagtgacctc caaaaggcag ttcaagtaga agagaaattg gcccgtgaag ctgcgactca
    11281 agtagtgaaa gaccaagtga ctgccgttta cgaagaaaaa tatgcggacc acgaagaatt
    11341 tgaccgtatt atgcgtgatg tggctgaaat cttggaacaa atggaacacg cagaagtgcg
    11401 acgtttaatt acagaagaca aggtgcgtcc tgatggtcgt aaggtcgatg aaatccgtcc
    11461 tttggatgcg gttgttgact tccttcctcg tgtacatggt tcaggtctct ttactcgtgg
    11521 gcaaactcaa gctctttcag tcttgacctt ggctccgatg ggagaaactc aaatcattga
    11581 tggtttggat ccagagtaca agaaacgctt tatgcaccac tataacttcc ctcaatattc
    11641 tgtaggggaa acaggtcgtt acggtgcgcc aggtcgtcgt gaaatcggtc acggtgccct
    11701 tggtgagcgt gctcttgctc aagtcttgcc aagcttggaa gaattcccct acgctatccg
    11761 tctagtagca gaagttttgg aatcaaacgg ttcttcatct caagcttcta tctgtgcggg
    11821 aactcttgcc cttatggctg gtggtgtgcc aatcaaggcg ccagtagctg gtattgctat
    11881 ggggcttatc tcagatggaa ataactacac agtattgaca gatatccaag gtttggaaga
    11941 tcactttgga gatatggact tcaaggttgc aggtactcgt gatgggatta cagcccttca
    12001 aatggatatc aagattcaag ggattactgc agaaatcttg acggaggctc ttgctcaagc
    12061 caagaaagcg cgttttgaaa tccttgatgt cattgaagca accattccag aagttcgtcc
    12121 agaattggct ccaactgctc cgaaaattga tacgatcaag attgatgtgg acaagattaa
    12181 gattgtcatc ggtaagggtg gagaaaccat cgacaagatt atcgctgaaa caggtgttaa
    12241 gattgatata gacgaagaag gaaatgtgtc tatctactct agtgaccaag atgctattaa
    12301 ccgtgccaaa gaaattattg ctggtttggt tcgtgaagcc aaagtggatg aagtttaccg
    12361 tgctaaagtc gttcgtatcg agaaatttgg tgcctttgtt aacctctttg ataagacaga
    12421 tgcccttgtt catatctctg agatggcttg gactcgtacc aatcgtgtag aggatttggt
    12481 agaaatcggg gatgaagttg atgttaaggt tatcaaaatt gatgaaaaag gccgtatcga
    12541 tgcctctatg aaggctcttc tacctcgtcc gccaaaacct gagcatgatg aaaaaggtga
    12601 aaagtctgag cgccctcacc gcccacgtca tcaaaaggat tacaaaccta agaaagaatt
    12661 tacagaaaca tcaaaagatt cagaataaga aaaggagaaa tgtatggggt ggtggcgcga
    12721 aaccattgat attgtaaaag aaaatgatcc agcggcccgc accactttgg aggttttgct
    12781 gacttatcca ggtgtcaagg ccttggcggc ccaccgtctc tcgcattttc tctggaagca
    12841 cggcttcaaa ctattagctc gtatgtacag tcagttttgg cgcttttgga ctcagattga
    12901 gattcatcca ggagcccaga ttgattcagg tgtttttatt gaccatggtt ctggtctggt
    12961 gattggagag acagcgattg ttgaaaaagg cgttcttctc tatcacggag tgactctcgg
    13021 gggaacaggg aaagactgtg gcaaacgcca tccgactgta cgaaagggag ccctcatatc
    13081 agcccatgcc caagttatcg ggcctgtgga aattggtgaa aatgccaagg tcggtgctgc
    13141 agcagttgtc gtagcagatg tacctagtga cgtgacggtt gtcggtattc cggccaagat
    13201 tgtccgtctt catggtaaga aagatgagcc tgtcattcac gaagtcgaag aaaaacgaga
    13261 gtattatgtc aataaactcg agcaggctaa agatgccagt cacagatcgt ctggtttgta
    13321 gaggatacct atatgattca agcaagaaac aagttaagcc aagaggagct atctgaggcg
    13381 aaaaaagtaa ttaactgttg ccaaaactat gacggtacct atcgcgatcc ctatctctct
    13441 aacatgctta attttgaccc aaacatgccc gcctttttcc tttattatga aaaaggcgaa
    13501 cttgttggtt tattaactgt ctatgcagat gaccaagatg tagaagtgac gatactggtt
    13561 catccaggtc atcgccgtca ggggattgcg cgtgcattgt ttactagttt tgagagagaa
    13621 acagcttctt tccccattcg gtcagtcact tttcagacag aacgtatttt tttagagaat
    13681 catcctgact ttgccagtaa ctggggacta atcgaggatg aagagacaga aacttggtta
    13741 ggtaaggata gaagacctta tccgttagca aatgtttccc atcttgaagt tttgttagca
    13801 gatagttcgt atcaggatca aattagtcaa ttaaaatttc aggcattttc agaggaacat
    13861 gaatcgagag aagttgtgga tagatatgtc gctgaagctc tgaaagatcc agaaagccga
    13921 ctatatattt tattaaaagc tggtcaggtt attggaactt gtacggttga tttatcgact
    13981 aatacgaatt acctctacgg tttagcaata ttggaacctg aacgtggaaa aggctatgga
    14041 agctacttag caaaatccct cgtcaaccaa ctaattgagc aaaatgacaa ggaatttcag
    14101 attgcagtgg aagatagcaa tgtaggtgcc aaacgtttgt atgaaaaaat tggctttgtc
    14161 aaacagactc aggtggttta tctgaatgag aaaggagcaa gggattccga agtgtagaga
    14221 tattcggact gaaatttact tgaactttta gtgatgaaac taattgttct tggatttcag
    14281 ctttcctgat tatgatttat gattaaaatc tatgacacca tgtctcgtga tttgcgagaa
    14341 tttgtcccga ttgaggacgg caagatcaag atgtatgttt gtgggccaac ggtgtacaac
    14401 tatatccacg taggaaacgc ccgttcgacg gtagcttttg atacgattcg tcgctatttt
    14461 gagtaccgtg ggtataaggt tgcctatatt tccaatttta cagatgtgga tgataagatt
    14521 atcaaccgtg ccagggaaga aggtatcacg cctcaggagg ttgcggataa gtacatcgct
    14581 gcctttcgtg aggatgtgac ggcattgggc gtgaaacctg cgactcgcca tccgcgtgta
    14641 gtggagttta tggcagacat catccgtttt gtggaagact tgatcgagaa aggctttgcc
    14701 tatgagagtc aaggggatgt ctatttccgt gtagaaaaat cccacaacta tgctaaattg
    14761 gctaataaaa ccttggaaga tttggagcta ggtgcttcag gtcgtaccga tgaagaaacg
    14821 gctcgtaagg aaaatcctgt agactttgct ctatggaaat cttccaaacc aggtgagatt
    14881 tcttgggata gtccttgggg acctggacgt ccgggttggc atattgagtg ttcagtcatg
    14941 tcgacagaga ttttaggtga taccattgat atccacggtg gtggagctga cctagagttt
    15001 ccacaccaca ccaatgaaat tgcccagtct gaagctaaaa caggcaaggc ttttgctaac
    15061 tactggatgc acaatggctt tgtcaatatc gacaatgtca aaatgtctaa gtccttgggt
    15121 aacttcatta cggtacacga tgcccttaaa actcttgatg ggcaagtact tcgtttcttc
    15181 tttgcgaccc agcactaccg taagcctatc aacttcacgg aaaaggcagt tcgcgatgcc
    15241 gagaccaatc tcaagtatct caagaatact tacgaacaac catttacagg aaatgtggat
    15301 gcccaagagt tacaaaactt taaagataag tttgtagcag ctatggatga agattttaat
    15361 gctgccaacg gtatcacagt tgtctttgaa atggccaaat ggatcaactc agggaactat
    15421 gatgcaagtg tcaagcaagc tcttgcagat atgttagaaa tttttggaat tgtctttgtt
    15481 gaggaagttt tggatgcaga gattgaagac ttgattcaaa aacgccaaga ggcgcgtgcc
    15541 aatcgtgact ttgcgacagc agaccaaatc cgtgaccaat tggttactca aggaattaag
    15601 ctccttgata ccaaggatgg agtgaggtgg acacgtgatt gatgtcaatc tcattaacgg
    15661 gattgcgcta gcctttgagg gggatgcggt gtattctatg tatattcgcc gtcacctcat
    15721 cctcaaaggt atgaccaaac ccaataaact ccatcaagaa gcaactaagt acgtgtcagc
    15781 caaggctcag gctcgcctga ttgctctcat gttggaggag caggtcctaa cggaaaaaga
    15841 agaagaaatc tacaaacgtg gccgcaatac caatagccac acaaaggcta aaaatgcaga
    15901 tgtcgtgact tatcgtatgt ccacgggatt tgaagcggtt atgggctatc tccatatgac
    15961 tgagaatctg gaacgtcttg agagtttggt ttcatggtgc atccaaaaag tggagggcta
    16021 gaacatgagg gcaaaagaac tacaagactg gtttcctgag gctcggattt cagaccaacc
    16081 agtagagaaa gagggctatc tcacgctccc tttagcttct cagcagtgga ttttgctgga
    16141 ggaagctggg ctcagcgagc gtgaaaagca gttggttgcc cttttgaccc agcaggagca
    16201 ggctcgttcg ctaaaccctt ggtattccta tctggttgag ggcaagggac aggcaccgca
    16261 agtttttaaa aagattcagt tggtttattg ccatctttct tattttcagc aggaaaatct
    16321 ggcttcttgg ctagatatga tgcggactct ttttccgaat tgtcagacag tgctacaggt
    16381 cggagctcag gattatgttt tcgtgcttca acaagacaaa tacacttctg taagagatat
    16441 tttaagtgat acgattgaag cggttgagta tgactttgga cttcgtcttt ctatcatgtt
    16501 gggtcaggtt tggtctcaga cgggacatca agccctatca gacttaatca aagctgagcg
    16561 ggatttgttc aagacatggt ggcgtcaggg tcaccaaggt gttcatactt tttctcagct
    16621 ctatctttgg agtatgggag aaagactcgt ggacttgaag ccaatcaagg aatgtctaca
    16681 ccagatgatt ttggatcaag atcagattca ggaaatcatt ctctctcttt gggaaaatag
    16741 tgctgttctc actaaaacag cccagcaact ctatctgcac cgcaattctc tccaatacaa
    16801 gattgataaa tgggaagagt tgacagggct tcagttgaaa gagttgaccg acctgacctt
    16861 gtgttatcaa ttgattttag gttctttgtc aactatagtt ggtttgtaaa gaagttaata
    16921 tttggagaag aggattgcca tcttctccat tttatgtgca gagttatagt ggcttgatgc
    16981 tgggaaagta cactgtgact gctaaaacat tcctagaagc tgctttgatt tccctaatct
    17041 atttatgcaa attttatgtt attttactat aacagttgtt gctaagccaa ataatagtgg
    17101 ggaagttcat ttagacgtaa gcattgaaga taatcaggga ggtagtgggt ataatttcag
    17161 ttctgtttca agtagctcac aaacagctaa atatgaagga actgtttata ataacaattc
    17221 atcattatat ataacgattg ataaaacgtc tgatgcaaca gctcttttga aattaaagtt
    17281 gaataatgtt gataatcaac ctgctactga agttcctagt tcaggaatta ctgtaaaatt
    17341 aaatgctaaa gataatgctg gaaactggac aagtgcttcg aataaaaaag aagtaacagt
    17401 aaaaattgtt tctgctaaac cgacatatcc agacaaaatc ttagtgaaaa atcctgataa
    17461 tataaaagat acagaaaaaa tgccattatt gaaaaattga aagaggcaaa taaaaatcat
    17521 ccagcaggag ctccaacctt tgctaaaggt gaaggagagc atgcaaatga tattgtagca
    17581 acttattcag atggtacaac ttattatgta ccgttaaatg atgtgacaaa atatgcgagg
    17641 tagtggctgt accactcact tattcacctc cccgtgattt gtagtagtga taggttttct
    17701 cactattatt ataaaacaaa ataaagatca caacactttt tcattctgtg ttgtgccttg
    17761 agtgaaacga aaggaatgaa ttataaatat gaaaagtata gtcac
//
LOCUS       DAVHEW010000038        17421 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVHEW010000038 DAVHEW010000000
VERSION     DAVHEW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.038"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(34..264)
                     /gene="blpU"
                     /locus_tag="VVL53_001144"
     CDS             complement(34..264)
                     /gene="blpU"
                     /locus_tag="VVL53_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU4183327.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGVARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(467..757)
                     /locus_tag="VVL53_001145"
                     /pseudo
     CDS             complement(467..757)
                     /locus_tag="VVL53_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(803..1036)
                     /locus_tag="VVL53_001146"
     CDS             complement(803..1036)
                     /locus_tag="VVL53_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4183328.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            complement(1042..2034)
                     /gene="plsX"
                     /locus_tag="VVL53_001147"
     CDS             complement(1042..2034)
                     /gene="plsX"
                     /locus_tag="VVL53_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU4183329.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMDLLKTAITGGGLRAKLGALLLKDSLRGLKKQIN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETEVVAQTASEFSGE"
     gene            complement(2031..2801)
                     /gene="recO"
                     /locus_tag="VVL53_001148"
     CDS             complement(2031..2801)
                     /gene="recO"
                     /locus_tag="VVL53_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU4183330.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2798..3967)
                     /locus_tag="VVL53_001149"
     CDS             complement(2798..3967)
                     /locus_tag="VVL53_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4183331.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            4116..5126
                     /locus_tag="VVL53_001150"
     CDS             4116..5126
                     /locus_tag="VVL53_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU4183332.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(5327..5404)
                     /locus_tag="VVL53_001151"
                     /pseudo
     CDS             complement(5327..5404)
                     /locus_tag="VVL53_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5445..5882)
                     /locus_tag="VVL53_001152"
     CDS             complement(5445..5882)
                     /locus_tag="VVL53_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU4183333.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5967..8600)
                     /gene="polA"
                     /locus_tag="VVL53_001153"
     CDS             complement(5967..8600)
                     /gene="polA"
                     /locus_tag="VVL53_001153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU4183334.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNVAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYDLAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFVWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            8856..9206
                     /locus_tag="VVL53_001154"
     CDS             8856..9206
                     /locus_tag="VVL53_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU4183335.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            9421..9537
                     /locus_tag="VVL53_001155"
     CDS             9421..9537
                     /locus_tag="VVL53_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU4183336.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            9530..9763
                     /locus_tag="VVL53_001156"
     CDS             9530..9763
                     /locus_tag="VVL53_001156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183337.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            9890..10171
                     /locus_tag="VVL53_001157"
                     /pseudo
     CDS             9890..10171
                     /locus_tag="VVL53_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10305..11273)
                     /locus_tag="VVL53_001158"
     CDS             complement(10305..11273)
                     /locus_tag="VVL53_001158"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU4183338.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(11418..12041)
                     /locus_tag="VVL53_001159"
     CDS             complement(11418..12041)
                     /locus_tag="VVL53_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU4183339.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSPFLNA
                     FESAIAAPLVEEPLKLLSLVFVLALIPVRKLKSLFLLGIASGLGFQMIKDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(12023..12232)
                     /locus_tag="VVL53_001160"
     CDS             complement(12023..12232)
                     /locus_tag="VVL53_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183340.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(12257..12754)
                     /locus_tag="VVL53_001161"
     CDS             complement(12257..12754)
                     /locus_tag="VVL53_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU4183341.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(12827..14188)
                     /gene="radA"
                     /locus_tag="VVL53_001162"
     CDS             complement(12827..14188)
                     /gene="radA"
                     /locus_tag="VVL53_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU4183342.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGFWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(14202..14717)
                     /locus_tag="VVL53_001163"
     CDS             complement(14202..14717)
                     /locus_tag="VVL53_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4183343.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(14719..15162)
                     /locus_tag="VVL53_001164"
     CDS             complement(14719..15162)
                     /locus_tag="VVL53_001164"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU4183344.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(15219..15316)
                     /gene="ffs"
                     /locus_tag="VVL53_001165"
     ncRNA           complement(15219..15316)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VVL53_001165"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(15349..15816)
                     /locus_tag="VVL53_001166"
     CDS             complement(15349..15816)
                     /locus_tag="VVL53_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU4183345.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(16016..17302)
                     /locus_tag="VVL53_001167"
     CDS             complement(16016..17302)
                     /locus_tag="VVL53_001167"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU4183346.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         5162 a         4298 c         3052 g         4909 t
ORIGIN      
        1 ctaaaccaat aataaaactt ttaaaatcca taattaccac caacatgtcg ctgcataggc
       61 cacacctcca agtatagctc ctcccgcagc accagttgct gcaccttgcc atgttcttgt
      121 tttaattcct agctgaagac ctcgtgctac tcctcctcca acacctgctt tggcaaaatc
      181 tccccaattg catccgccac cttcaacgca agcaagcatc tcagtatcca taatttcaaa
      241 ttgtgacatc ttttttgtat tcataacgaa tacctctttt ttatttttaa tatttgtctt
      301 gttacaaact tgacaagttt agtataacag tatctattaa tttttttcat ccaaatcttg
      361 aattggcatc gaaacgtctt gaattagctt ttttgtttca aaatcatctc tatttttaaa
      421 aaagatgttt tctaatcact tttttactat tagacttcct gcaaaactag aatcctagtt
      481 catgattgat aataccagca atcaaattca ttcgtaatcc gaagcgttta cgatgatttc
      541 gataggttgt tgaaaacatt ttaaacgttt ttactttggc aaagatattc tcaaccttgt
      601 ttctctcctt agatagcgca tggttacagg ctttatcttc aactgttagc ggcttgagtt
      661 tgctggattt acgtggagtt tgtgcttgag gatatatctt catgagccct tgataaccac
      721 tgtcagccaa gattttacca gcttgtccga tatttctcat ttctaaaaac catctacttc
      781 cgttgacttg gaactccgat tgctattttc cttgaatgat tttaaccaca tctcctacac
      841 tttggagttg gtcaatttcc tcatcgctga tttcgatact aaattcatcc tccagcgtca
      901 agataaactc catcaaatca actgagtcag catccaagtc gtctttcaga ctcaaggatt
      961 ctgtcacgac aaaatcctct ccctgtctct cttggataat ggtcacaata cggtcaaaaa
     1021 tttctttttc tgtcatctct tttattctcc tgaaaattca ctcgcagtct gggcaactac
     1081 ttctgtttct agcatggtac gaatctggcg aatcgtacta taaacagcct tggcatcgct
     1141 tgagccatga gtcttgacaa caggtgcctt aacaccaaac aagactgctc caccaacatc
     1201 tgaatagttg atctgttttt tcaaacctct gaggctgtcc ttgagaagga gtgcacctag
     1261 tttcgctcga agaccaccac ctgtaatagc agtcttgagc aagtccataa ttcccatggc
     1321 tgtcccttca atagatttga gcacagcgtt tcccgtgaaa ccatctgcca caacaacatc
     1381 cgcaacgcca ttcatcaaat cacgtgcttc cacgtttccg ataaagttca aactttcatc
     1441 agccgccagt aattcataag tttccttacg aagcgggtcg cccttgctac tctctgttcc
     1501 gttgttgagc aaaccaacgc gtggttgcgc aatgccacgg acatttttag catagaagga
     1561 acctagaacc gcatattgat ggaggtgctg ggctgtattt tctgcattgg caccaaggtc
     1621 tagcatgtca aaaccttttc catcaacggt aggcaaggta gacatgagtc caggacggtc
     1681 gatattcttg atacgaccca cgatgaagaa tcctgctgcc aacaaggcac ctgtattccc
     1741 agccgaaagg acagcgtctg cttcaccatc tttgacagcc ttggctgcca ataccatact
     1801 ggcatttttc ttattccgaa tagctctcgt aggttcatca tccgaatcaa tcttctcatc
     1861 cgtatggata atgctgacgc gctctgtcgc tgtcagatat tgcttgattt tagcttcatc
     1921 tccgtaaagt tgaacctcga tatctgaaaa gtcagatagg gcttgattga caccctcaac
     1981 aatggcctga ggtgcgtaat cgccccccat ggcatctact gcgatttttt tcatttcttt
     2041 tcctctttta gtaattgtcc ccagtctgct agggaatcaa taaatttctt tgattttagg
     2101 tgaatcccaa cgtactcttc atataattga tccataaatt ggcgtagctc ttgcttgatt
     2161 ccaggcttga gcgaaatggt ctccaaagtc tcaaaatcaa tagcttgaaa ttgattgagc
     2221 agatagggga tattgggatt gagatgacaa cgtctcttat cctcatgata atgctctgga
     2281 cagaggcagg ctccatattt gaaagaaaag tcaaaagcct gaccaacccg atggcagaag
     2341 acacactcat taaaattgag gctgattcca aatcgagtca aaatttgaat ttcaaaaata
     2401 ttggtcaaaa cctgataatc caagcctgct tccatcaact ccaaagtctt ttgcaaaaaa
     2461 gcaaacaagg gagcatcctg ctgattgtcc tgcaaactag catctgcaag agctgccaca
     2521 taggtcgcat aggccatgac aaagaggtca ctattaatct tgggaaaagt catgacctca
     2581 tgatagtctt cgatgtaact gagtccgtca tcattgattc gcaagagaaa tcgtgccagc
     2641 accaagggct gaataacagg cgccagctta gactgaccag cgtgtttgac aaaaaacatg
     2701 catttgccaa cctgctctgt aaaaattttg acaagcttgt catcctcacg aaaattgcga
     2761 ttgtaaagca ccaagccttg actcgtgata gactgaatca tgcttctctc atatactcct
     2821 caagtcgttt catggcttct ttgatagtct ccatgctagc tgcataagat aggcggacgt
     2881 agccttcccc gtaacgtcca aaggctgcac cagggataaa ggcaacggcc ttcttctgag
     2941 caaaatcctt cagaaaagca aaggagtctt gattgtagcc cgctggaatt ttagcaaaaa
     3001 tatagaaggc accgtctggt ttgataatct caaaaccaag agcagtcatt ttttcgatga
     3061 tatagtcccg acgctggata tattccttct tcatgggctc cgcatcattt ttaccagccg
     3121 tcaaggcttc taccgcagca tgttgcgcca tggtatttgc ggcagtgacc aagtattggt
     3181 gactcttgat taactgggct gtgaaggccg caggagcgaa aatcagcccc aaacgccaac
     3241 ctgtcatggc atgcgattta gacaaaccat tgataataat agcctggtct ctcaacatcg
     3301 ttcctagaga cacatgggct tcgcctgtgt aggtcaattc tgagtaaacc tcatcacaga
     3361 caacaaaaat ttcgtacttg cgtaaaacag ctgccaaggc ctctaactgc tctcgactgt
     3421 aggtaattcc tgtcggattg gctggatagt tgagaataac cgccttgagc ttatcaccct
     3481 gctccaaaat ggccttctcc aacatctcag gagtcaagac aaaaccattt tcagtcgtat
     3541 caatctcaac aatttctgcc ccaactaagt taacaatcgg ttcatagcct ggataagcag
     3601 gagctggcaa aagtaccttg tctccctctt ccaaaatagc cgtcaaagtc gcagataaag
     3661 cctctgtcgc cccaattgta accaagattt cattttcagg agcatagtcc agttggtact
     3721 tttccttaac aaagtcacta gctgcctgac gtagagtcag cagaccactc atccctgtat
     3781 agtaggattg gttctgatca atcgctcgct tggccgcctc cttgacatgg tctggcgttg
     3841 taaaatcagg ttcccccaag gtcaaacgca agaccccagg aatctccgaa atagcctggt
     3901 caaactgacg aatcaacgaa acttgaattt tatctaactg tttattaaag cgcttagtta
     3961 agtccataga tacctcctac tttcaaaacg tatctctatt atactacaaa agccccccga
     4021 ccgcaaaaat acattagaga atagctttcc actttctatt ttacttttcc tgtttatagg
     4081 tctataatgg taatagattt tatttggagg tttttatgtc atttctatca aaaaatggag
     4141 caggtatctt ggcctgcctt ctcatttcta tcgtatcttg gtacttagga ggattcttcc
     4201 ctgtgattgg cgcgcccgtt tttgccattt tcataggcat gctcctacat ccctttctct
     4261 cgtcctataa acaactggat gctggtttga cctttagttc caagaagttg ctccaatatg
     4321 ccgttgtctt gcttggtttt ggtctcaata tctcgcaggt cttcgcagtt ggccaatctt
     4381 cactccctgt catcctgtcc actatctcaa tagctctgat tattgcctac ctcttccagc
     4441 gtttctttgc cctggataca aaactggcta ccttggttgg agtaggttct tctatctgtg
     4501 ggggttctgc cattgcagcg acagcgcccg ttattgatgc taaggaaaag gaagtagccc
     4561 aagccatttc cgttatcttt ttcttcaatg tcttggctgc gctcatcttt ccaaccctcg
     4621 gcacctggct tcatctatcc aatgaaggct tcgccctctt tgcagggact gcggtcaacg
     4681 acacttcctc tgtaacggct gccgccagcg cttgggacag tctttaccaa agcaataccc
     4741 tcgagtctgc aaccattgtt aaactcacac gtactttggc cattatccct atcacgctct
     4801 ttctatccta ctggcaaagt cgccaacaag aaaacaagca aagcctgcaa ctgaaaaaag
     4861 tcttcccact ttttatcctt tactttatcc ttgcctctct cctcactaca ctactcacct
     4921 ctctaggtgt gtccagtagt ttctttactc ctctcaaaga actctctaaa ttccttattg
     4981 tcatggccat gagtgctatc ggtctcaaaa ccaatctggt cgctatggtc aaatccagtg
     5041 gaaaatccat tcttcttgga gccatctgct ggattgccat catcctcacc actcttggta
     5101 tgcagaccct tatcggcatt ttctaatact cttcgaaaat ctcttcaaac cacgtcaacg
     5161 tcgccttgcc gtaggtatat gttactgact tcgtcagttc tatctgcaac ctcaaaacgg
     5221 tgtttgagct gacttcgtca gttctatctg caacctcaaa acggtgtttt gagctgactt
     5281 cgtcagtcgt atctacaacc tcaaaacagt gttttgagct gacttcgtca gttctatctg
     5341 caacctcaaa acagtgtttt gagcagcccg tggctagttt cctagtttgc tctttgattt
     5401 tcattgagta taacacaaaa ggtagcccat cagctacctt tttcttatgc ttcctcaatc
     5461 aagcgagtat gttctctctt gatacagcga ttcatcacga tatcatcaca tccaccatca
     5521 cgcaaaatct ctttcgcttc taaactttca agtcctagct gtgcccaaaa aatcttggca
     5581 tcagctttga gaaaatcacg cgccacatcg ggcagaaatt cactgcgacg ataaacattg
     5641 acaatatcta caggaaaagg aatttcagcg aggctagcat aagccttttc acccaagatt
     5701 tcgccacctg ccgccttggg attgactggg atgattttat agccccgagc ctgcatttcc
     5761 tttgttactc gattgctggt tgtttcttca cggtcagaca aacccaccac agcaagggtt
     5821 ttactcgttg cgagatactg acgaatcacg ccatcacttg gattgataaa ttcttgactc
     5881 atagaaatcc tcctttttca tcagtatagc acattttgaa aaggtttgca gaattatact
     5941 acaaaaaagg aggactagcc ccctttttat ttagcctcgt accaggttgc cccttcattc
     6001 tcatctgcga taagaggaac actgagttga atggcttctt ccatggtttg tttcaccaat
     6061 tttttcatct ctaccaattc agatttaggc acttcaagga cgatttcatc gtgcacttgt
     6121 aacagcatct tagtctgata accacctgca accaaggctt tatccagctg aatcatggca
     6181 atcttgagaa tatctgctgc cgaaccctgg ataggtgagt tgatagcagt tcgctccgca
     6241 aaaccacgaa tattgaagtt gcgcgaattg atatctggca actcacggcg acgcttaaag
     6301 agggtctcta catagccctt atcacgcgcc tcccgcacca cttcatccat gtagttttta
     6361 atacctggaa aacgttcaaa gtaggtatca atgtaggctt tggcttcctt acgactaatt
     6421 cccaaattat tagacaagcc aaagtctgaa atcccataaa ccactccaaa gttaactgcc
     6481 ttggcattgc gacggtcgtt tgcagtcaca tcatcaggac gctcaatgcc aaagacccgc
     6541 atggctgtcg aagtatggat atctgccccc tcttggaagg ccttaatcaa gtgctcatcc
     6601 ttagaaatat gcgccaaaac gcgcaattca atctgtgaat agtcagagct gagtagcaca
     6661 ctatcctccc actctggcac aaaagccttc cgaatcaagc gcccctgttc caatcgggca
     6721 ggaatatttt gcaagtttgg atccacacta gacaaacgcc cggtctgggt caaatcctgc
     6781 acatagcgag tatgaatctt tccatcagcc aaaatccagt cctgcaagcc aattacataa
     6841 gtagattgaa tcttagcaat ttgacggtaa tccaggattt tcttaacaat cggagcaata
     6901 ggagcgagac gctctaaaac atccactgct gtcgaataac ctgtcttggt tttcttagtg
     6961 tattctagag gaagtcccaa tttctcaaag agaagcacgc ccaactgctt aggcgagttg
     7021 acattaaact cctcaccagc cagctcgtaa atctcttgag tcagtttttc aatgacaagc
     7081 tcattttcag cctgcatctc aagcaaggtc tctttcttga ccataatccc agcaatttcc
     7141 atcttggcaa ggacaaaagc cagaggttgc tccatatcat aaagaagctc taattgccca
     7201 ttttcgctga gtttttcaag taaaataggc tctgtttcta ccaaaacagc aagtttacaa
     7261 gctaagtgtt ccaagaattt ctcacgttca ggaatggcct ttttaacacc cttaccgtag
     7321 aaagtttcat catcaaccaa gtaagtctga ccataaagac tagcgatggt cgcaatttca
     7381 ttgtcctcca cagtcgaaag gaggtattta gccaaacgga tgtcaaaagc aggcgcctgc
     7441 aaatccacac caaaacgttg caaaagaact ttaaccttct taaagtcata aactctcaga
     7501 gatgtttttt ctaagaaatc cttgaaaatc gggtcttgca acagctcaag cttgtctgtg
     7561 gcatagagct tatccccaca agaccagaca aatccaacca aattatccgt atggtaattc
     7621 tcaccaaaaa gctcaaagtg gaagatagac tcttcactca gcatatcttg actgatttgg
     7681 tcaacaatag taaaatccaa actctcagac acatcagctg acgacacatt taaagcctgc
     7741 tttagctgtt tgaagcccat ctcatcgtag aatttcccaa gattttcaac atctggacca
     7801 ctatagacca agtcctctaa accaatcgca atcggtgcct tggtatcaat ggtcgctagt
     7861 gttttagaca aaaaggcctg ttccttgtca ttgatgagat tttccttcat cttagaagtc
     7921 ttcattccat caatattttc ataaatcccc tcaagcgaac catgctccag caagagctta
     7981 atacccgtct tttcaccgac tttggtcacc ccagggatat tatccgactt atcacccatg
     8041 agcgccttga gatcgataaa ctgagctggt gtgaggccca tttcttccat gaggtaatct
     8101 ggcgtaaagg cctcaaactc agccacacct ttcttggaaa tttcaaccac cgtatgctca
     8161 tccgtcagct gaatcaaatc cttgtcccca ctgacaatag taatatcaaa accatcctgc
     8221 tctgctagct tatccagcgt cccaatgatg tcatccgcct catactgagc cagatcatag
     8281 tgacgaatcc ccatatgatc cagcaactca cgaatgaaag gaaattgctc acgaaactca
     8341 tcaggagtct tggcccgacc acccttatag tccgcataca tctctgtccg gaaggtcgtc
     8401 tttcccgcat caaaagccac caaaatatga ctcggctcaa cccgctccaa taaatgactc
     8461 aacatcaact gaaaaccata aatcgcattg gtatgcaaac cagccacatt cttaaaacgg
     8521 tccaactgct gatacagcgc aaaaaacgcc cgaaaagcta cagaagaccc atcaatcaat
     8581 aataattttt tcttatccat acacccatta taaaggaaag aatcaaaaaa taccattggg
     8641 aagagctaga gcaagtattt ttcaaacttt ttccgaataa atagatagag ccagagaatt
     8701 tagtaaacct agatttaaaa atgtgctata atatagtata ttgaatctat aatagtacac
     8761 cttgactgct aaaatatttc tataaattaa tttgactttc ctgatagagt tattcacatc
     8821 ttatttcaac tcactataga aggaggaata ggaggattct cagacatccg ggcatcagcc
     8881 caactaatga tttgattgct aagaaaatat tcagcaatcc agaaatcact tgtcaattta
     8941 ttcgcgatat gctggacttg ccagcaaaaa atgtgaccat tttggaggga agcgatattc
     9001 acgtattact ctccatgcct tactcggtgc aggattttta taccagtata gacgtcttgg
     9061 cggagttgga taacggtact caagtaatta ttgagattca agtccatcat cagaattttt
     9121 tcatcaatca cttgtgggct tacctgtgca gtcaggttaa tcaaaatctt gaaaaaattc
     9181 gtcagcgaga aggtgatact cactagagct acaaacacat cgctcctgtt tacgccattg
     9241 ctatcgtgga tagtaattat ttctcagatg acctggcttt tcatagcttt agtatgcgcg
     9301 aagacacaac aggtgaggta ttggcgatta ccaacaatgg acaggaaaac catctggtta
     9361 agatggcatt cttggaatta aaaaatacag agaaaccagc aaagacaagg ttcgcaagcc
     9421 atggttggag tttttcggca acaagccctt tacccagcaa ccgcaacgag ccattaccca
     9481 agcaaatcaa ctgctggact acaagagctg gtccgaggag gacaggaaaa tgtttagtca
     9541 actacatatg cgagaagaac aagtcttgtt agcacaggac tatgccttgg aaactgctag
     9601 ggctgaaggc cttgaacaag gactagagcg tgggaaagtt gaaggaaggg cagaaaggaa
     9661 actttttgcc ttcctagaca tagtacgcca aggtcttctg acttctgagg ttgccagcca
     9721 gcaattaggt atgtcagtat ctgaatttga ggcactgttg taaaatggct ccataatatc
     9781 catagtgggt aaatccccta tggatattat ggagcctatt ttgtgtagaa aaaaagtccc
     9841 atatgaccta taatgaaaag cgacaaaaca actcattaga aagaatcata tggaacaatt
     9901 acattttatc acaaaattac tagacattaa agaccctaat atccagattt tagacatcat
     9961 caataaggat acacacaagg aaatcatcgc caaactggac tacgacgccc catcttgccc
    10021 tgagtgcgga aaccaattga agaaatatga ctttcaaaaa ccttctaaaa ttccttatct
    10081 tgaaacgact ggtatgccta caagaattct ccttagaaag cgtcgattca agtgctatca
    10141 ctgttcaaaa atgatggtcg ctgaaacttc tgatgacgta cagtcatatt tcttctcttt
    10201 ttattatatc acagttttaa atctagcttt actagattca ccgctactat ctatttattc
    10261 ggaaaaaaga cgaaaaaacc tgagaatcat ctcaggcttg gtcattaaat ttttttctca
    10321 atatcgaaaa gtggagaaag tggtcgtttt tcatgaatac gtacgatagc atcccctagg
    10381 agatgagcga ttgaaatctg ctcaatctta tcaatcaaac gctcttctgg cagatagatg
    10441 gtatccaaaa caaccaattt cttaatagct gatttttgga tattgtccgt agcaggacca
    10501 gaaagaactg ggtgcgtaca gcttgcatag acttcaacag caccagcttc cgcaagagca
    10561 tctgccgcat gacaaatcgt tccagcggta tcaatcatat catcaatcaa gatacaagtc
    10621 ttgccttcaa ccttaccgat gatattcata acttcactag tattcatctt atcaacgcta
    10681 cgacgtttat caataatagc gatagatgtt ttcaaaaatt ctgccaactt acgagcacga
    10741 gtcacccctc catggtccgg gctgacaacc acatagtcag aaccaaccat accacgacgc
    10801 tcaaaataat ctgcaatcag aggagcaccc atcaaatgat ccacaggaat atcaaagaat
    10861 ccttgaattt gcgcagcatg caagtcgatg gtcaataaac gatccactcc agctacttca
    10921 agcatatttg cgacaagttt tgaagtgatt ggctcacgcg ctctcgcctt tctatcctga
    10981 cgtgcatacc catagtaagg catgacaaca ttgacagatt ctgcactcgc acgcttcaaa
    11041 gcatctacca taatcaaaat ttcaagcaga ttgtcattta caggcgaact agttgattgt
    11101 aagataaaga cgtgtttccc acggattgat tcttcaatgt tgacctgaat ctctccatct
    11161 gaaaattggc gaacacttga tttccccaac tctatcccaa tctcctgcgc cacacgttct
    11221 gccaattctt tattagaaga aagggcaaac agctttaaat cagaaaaaga catgatttcc
    11281 tccggtatat atgtataact tgtgcttttc acaagatttt ccatctacca ttgtagcgct
    11341 ttttgcacta tttttcaatc aaaaataaaa gaagggcacc atatttgtac ccttgcatca
    11401 ttcttttgaa aaatattcta ggtcatcaac tcattgtgtt tctcaacaaa gcaataagca
    11461 tgataaaaac catagagagc aatagccgta accactggaa tcgctaaagg caactctgtt
    11521 tccaactcca caaaaggaga gttaaacaag aagtgagttc ccaaggctaa acctagaaaa
    11581 ataaggccct gtttcttgcc aaccttctgt cctttatagg ctctgtaaag caagtaaaca
    11641 cctactacag ctagacctga aaaagtccag tgagaggcaa ttcctgagat gatacgctct
    11701 aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat ccgtacgaat ataaccaata
    11761 tccttaatca tttggaatcc caaaccggaa gcaattccaa gtaaaaacaa agattttaat
    11821 tttcgcacag gaatcaaagc caaaacaaaa acaagtgaca ataatttcaa gggttcttct
    11881 accaaaggag ccgcaatagc actttcaaag gcatttaaaa atggactatc tgggaaaaga
    11941 acccccagta aatcatggat ataagtatta gcaaaactag acaaccagcc tgaaaggaac
    12001 atccctccca ataaagacag aatcaaaacc ttctttggca attcccattt ttcccaatac
    12061 ggaagagaaa ataaagagcc ggaatcatgt aaaagagagc tagaaagata gaaactccca
    12121 ttagtccata ttccgcacct gacctcgaac cgtccgtata gtagatggtt tcatactgta
    12181 aaccaataca tagcaataaa ataaaaataa ataaaatact gcttttcttc atacactttc
    12241 tttctaaatg aagtatttat aattctacga ctgtcatact tcctgtatca acattgtaaa
    12301 tggcaccaga gataatgaca tcgtctggta ttaggggaga ctcgataagc agttgcatat
    12361 cctcgcgtac actctcttct atatcttgga agggcaagaa gtcctggtct gacacatcga
    12421 cacccaattc ttccttcaaa tactcctgaa aaggttcatt ttcaaaggtc tgagcaccac
    12481 agtctgtatg atgcaatacc acaatttctc ttgtccccat ttgttgctgg gaaataacta
    12541 gagaacgaat catatcctca gtcactcgac cacctgcatt ccgcaaaata tgagcatccc
    12601 caagtgccaa acctagagct tgcgcaacgt gcaaacgtga gtccatacag gtcacaatgg
    12661 ctactctggt tttaggttta agtggcagat ttaactgccc atgtagggca acataagcct
    12721 gattggcttg cataaactgt tcaaaatacg acacgattcc ctccttgaaa atttgatagt
    12781 caaatatttc tcctatctta tcatttttaa gagaatttgt cacggattat gcaaagacct
    12841 ttttcaagac ttcctgaatc gttgtcacgc caatgacctg aatttcctta ggcagagtga
    12901 ttcctgtcaa ggaattctta ggtacataaa tcttagtaaa gcccagttta gcagcttcgt
    12961 tgatgcgttg ctcaatacga ttcacgcgcc gaatctctcc tgtcaagccc agttctccga
    13021 caaaacattc ctgaggatta gttggcttgt ctttgtagct cgaagcaata gcaactgcaa
    13081 cagccaagtc aatcgcaggt tcatccaatt taacaccacc agcagatttg agataggcat
    13141 cctgattttg caagagaagc cctgcccgtt tttccaaaac agccataatc aagctagcac
    13201 ggttaaaatc aagtcctgtc gtagtacgct tggcatttcc aaacatggtc ggtgttacca
    13261 aagcctgaac ctccgccaaa atcggacgcg tcccttccat ggttacaacg atggaggaac
    13321 cagtcgcccc atccaaacgc tcttctagga aaacttgact cggattgagt acctcaacca
    13381 agccgcccga ctgcatctca aaaatcccaa tctcattagt ggaaccaaaa cgatttttga
    13441 ccgctctcaa aatacgaaag gtgtggtgac gctccccttc aaagtaaagc accgtatcca
    13501 ccatatgctc caacatacga ggcccagcca aggttccttc tttggtcaca tgacctacga
    13561 taaagatggc aatgttattg gtcttggcca actgcatgag ttcagcggtc acttcacgca
    13621 cctgagaaac agacccctgc acccctgaaa tctcaggaga catgatggtc tggatggaat
    13681 caataatgag aaagtctggc tggatacgct ccacttctgc acgaacactc tgcatattgg
    13741 tctctgcata gagataaaac tcactatcaa tatcacctaa gcgctctgca cgtagtttaa
    13801 tctgctgggc agactcctcc ccactgacat agagaactgt ccccacttgg gacaactggg
    13861 ttgagacttg taggagaaga gttgatttcc caatcccagg atccccaccg ataaggacga
    13921 gacttcctgg taccactccg cctccaagca cacggttgaa ttcctccatc tccgtcttgg
    13981 ttcgattgac attgatggaa gtcacctcag ctagtttcat gggcttggtt ttctcacctg
    14041 tcaaggacac acgcgcattc ttaacttcgg caacctcaac ctcttccaca aaagaagacc
    14101 aaaacccaca gttggggcaa cgtcccagat atttagggga attataccca caattttgac
    14161 atacaaatgt cgcttttttc tttgcgatga caaacctctt tctatatctc taactcacac
    14221 tcaatcactt ggcaaaaatc aatcttctca tttggcacaa actggcgcat gagcattcga
    14281 tgagcaacaa ctaccacagt ctgatgttct cgatacttag acatacattc tagaaaccga
    14341 gacttcattt ccgtagctgt ctcatattga ataggactat taggaagcaa ctcccccttg
    14401 ttttctaaaa acagtcttct agctgtttca aagttttcta ttcctgtttt atagacctgc
    14461 cattcatgta ataaaggctc tactcttaaa ggaagacccg tagcacagac cacatacgaa
    14521 gccgtttcta aagctcttgt gactgcagaa gatacgatta tttcagctga cgagagtaaa
    14581 ggatttttgc tcaatttctg gacttgctgc cgtcccatct cagacaaggg tgccaaatct
    14641 atcccaaatc ctatataaga acgctcctct aactcacggt aatctggctc cccatgacgt
    14701 acaaagataa tcttcattct agtgccctgt cgatccaaat ccaccagttc gaacgccatc
    14761 agctgcatct ccatctgcaa ttaagaaagt agcaaaaaca gcctggacaa tacgctcccc
    14821 aacttcaaga acaacctctt ggtctgtgat attcttcatc tgcgcaaaaa tatgcccttc
    14881 atttccagga tttccataat aatccccatc aatgactcca actgagttaa ttaaaaccaa
    14941 gcccttctta cgaggatttg aagaacgatc atagaggtag agaacctcag tcggctgcat
    15001 ataagcctta acccctgtcg gaaccaagac aatctctcct ggcgcaacaa ctgtacgcac
    15061 agcaaccttt aagtcgtaac cagccgcatg cgctgtctca cgcttgggca ataaattttc
    15121 atctgtaaaa ctcgaaacca attcaaaacc acgaattttc ataattttct cttttctatt
    15181 atcatttatt ctagattatt ctatcttatt tattcggaaa aagcacgaaa aaaagagcac
    15241 acaattcaaa tcgcttaggg ctgctggatt cctcccctga cccgcttcac gcagaactgt
    15301 tgctccacta tttattatat cacattccta ttcattttaa aagcaaaatt attttttccg
    15361 tctatttcta aaaaaatcct gcatgatagc tgcgcattca tcttccaaaa ttcctatttc
    15421 aacctccaca cgatgattga gacgctcatc tgtcaagata ttgtacaaac tcccagcagc
    15481 gccaaatttc tggtttttag ccccatagac cacatttgga atgcgggcaa gcccaatcgc
    15541 tccactacac atgacacaag gttcaatggt cacaaaaagt gtgcaatcca gcaagcgcca
    15601 gctctcctca ctcaagttcg catcctctat agccataatt tccgcatgca taaccgctcg
    15661 ctgtaattcc tcacgcgcat tatgcccacg accaatgatt tccccatctt tgacaatcac
    15721 acaaccaatt ggaatttcat cgtgttcaag agcaatctca gcctctctca aagcctccct
    15781 cataaagact tctttttctt caagcgtata atacatctct tttctctttt cctacttatc
    15841 gattttatta ttatatcatg aatccaacac aaaaaaagcc accgaatgcg gtgactctat
    15901 agggagatta ttatgaaaaa gaaaagttta ggatatttgt tacaacaagt taggaggtct
    15961 tcttgtaacc gtctatagta tacccgacct atcttaaaca aatcttaaaa atctcttagg
    16021 accaaacact ttctaaaata tttgtttgtt cacgaccagg acctactgag aaagtagaaa
    16081 tacgaacgcc aaccaattca ctcacacgac gaacatagtt acgcgcattc tcaggaagat
    16141 cttccaaatt gcgaactccg gtaatatctt ctgaccaacc tggcaactct tcatagatag
    16201 gcttgcaacg tttcaattgc tcaagactag ctggatagta gtcaatacgt tgaccgtcaa
    16261 gatcataggc cacacagatt ttcacagtat ccaaaccgct caaaacatca atagagttca
    16321 aagaaaggtt agtaatacca gaaacacgac ggctatgacg catcacaact gagtcaaacc
    16381 aacctacacg acgtggacga ccagttgttg taccatactc atgacccact tcacggatac
    16441 gttctcccac ttcatcaaac aactcagttg ggaaaggacc atctcctaca cgactcgtat
    16501 aagctttaca tacacctaca accttgtcaa tcttgcttgg accgacacca gaaccaattg
    16561 tcacaccacc agctacaggg tttgatgacg taacaaatgg ataagtacct tggtcgatat
    16621 ctagcataac accttgtgca ccttcaaaaa gcacacgttt gccattatca agcgcatcat
    16681 tcaagataac agatgtatct atcacgtatt tcttgatttg ttgaccatat tcgtaatatt
    16741 cttcaaaaat atcatcgaaa acaatcgctt tactgtcata caatttttca aaaagacgat
    16801 tcttttcagc aaggttacgt tctaaacgct cacggaaaat atctttatct aaaagatctg
    16861 caatacgaat tccaacacga gcagccttgt ccatataagc tggaccaatt cccttaattg
    16921 tcgtaccaat cttattgtcg cccttagctt cttcttgcaa gcgatccaac tcgatatgat
    16981 aaggcaaaat aacatgcgca cgatcagaaa tacgcaagtt atcagttgtt acaccttcct
    17041 catgaagata gctcaactct tttacaagag atttaggatt tacaaccata ccattcccaa
    17101 tgacagatat tttttcaggg aagaaaatcc cagatggaat caagtgcaac ttaaatttct
    17161 taccgtcaat cacaatcgtg tgaccagcat tatcaccacc ttggtaacgt gcaatcactt
    17221 ctgcattcgc tgaaaggaag tctgtaatct tccctttacc ttcatcaccc cattgggtac
    17281 ctacaacaac aactgaagtc ataattttgt ctgagccctc aggctcttcc tttctcacat
    17341 acatggcagg actctcacct gcaattatat cttacaattt attataataa aaaatcgcct
    17401 ttttatcaag aagaaacaat a
//
LOCUS       DAVHEW010000039        16758 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVHEW010000039 DAVHEW010000000
VERSION     DAVHEW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.039"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            389..1567
                     /locus_tag="VVL53_001168"
     CDS             389..1567
                     /locus_tag="VVL53_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4183347.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMEIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            1881..2012
                     /locus_tag="VVL53_001169"
     CDS             1881..2012
                     /locus_tag="VVL53_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU4183348.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2033..3583
                     /gene="dltA"
                     /locus_tag="VVL53_001170"
     CDS             2033..3583
                     /gene="dltA"
                     /locus_tag="VVL53_001170"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU4183349.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3580..4824
                     /gene="dltB"
                     /locus_tag="VVL53_001171"
     CDS             3580..4824
                     /gene="dltB"
                     /locus_tag="VVL53_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU4183350.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIDLRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4838..5077
                     /gene="dltC"
                     /locus_tag="VVL53_001172"
     CDS             4838..5077
                     /gene="dltC"
                     /locus_tag="VVL53_001172"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU4183351.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5070..6338
                     /gene="dltD"
                     /locus_tag="VVL53_001173"
     CDS             5070..6338
                     /gene="dltD"
                     /locus_tag="VVL53_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU4183352.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMNYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6493..6933
                     /gene="adcR"
                     /locus_tag="VVL53_001174"
     CDS             6493..6933
                     /gene="adcR"
                     /locus_tag="VVL53_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU4183353.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6933..7637
                     /locus_tag="VVL53_001175"
     CDS             6933..7637
                     /locus_tag="VVL53_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU4183354.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGNKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVSHA"
     gene            7630..8436
                     /locus_tag="VVL53_001176"
     CDS             7630..8436
                     /locus_tag="VVL53_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU4183355.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8446..9951
                     /gene="adcA"
                     /locus_tag="VVL53_001177"
     CDS             8446..9951
                     /gene="adcA"
                     /locus_tag="VVL53_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU4183356.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGKEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11111..11884)
                     /locus_tag="VVL53_001178"
     CDS             complement(11111..11884)
                     /locus_tag="VVL53_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU4183357.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            12060..13463
                     /locus_tag="VVL53_001179"
     CDS             12060..13463
                     /locus_tag="VVL53_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU4183358.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13578..14216
                     /locus_tag="VVL53_001180"
     CDS             13578..14216
                     /locus_tag="VVL53_001180"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU4183359.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            14228..14671
                     /locus_tag="VVL53_001181"
     CDS             14228..14671
                     /locus_tag="VVL53_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU4183360.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENVQ"
     gene            14661..15092
                     /locus_tag="VVL53_001182"
     CDS             14661..15092
                     /locus_tag="VVL53_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4183361.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15083..15553
                     /locus_tag="VVL53_001183"
     CDS             15083..15553
                     /locus_tag="VVL53_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4183362.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15578..16351
                     /locus_tag="VVL53_001184"
     CDS             15578..16351
                     /locus_tag="VVL53_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU4183363.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16348..>16758
                     /locus_tag="VVL53_001185"
     CDS             16348..>16758
                     /locus_tag="VVL53_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4183364.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIF"
BASE COUNT         5060 a         2879 c         3530 g         5289 t
ORIGIN      
        1 actcccctga aatttatttt aattatgcaa atttcacgta tttttgatgc tgagacgacg
       61 atcctgggaa cttttcagat atttttttga ctatctaaat ctatcattag aaaagcttag
      121 agcgccaaag gatttgagcg tttttctgat ttttaagact ttttccagtc tctttttcga
      181 ttgaagatgt aattattcta ctaactaact aacttcttag tactagccaa caacgataat
      241 cataattcct cctaaaatta ggaataataa aggcaatagt ttttgttttt tcatgtaaaa
      301 aacctcactt ttgttttctg ctattttatg ctaaaatatt aaaaatcaaa tttaattcca
      361 aagtttgtaa ctaaaggggg agcgctacat gtctaattca tttgtcaagt tgttagtctc
      421 tcaattattt gcaaatttag cagatatttt ctttagagta acaatcattg ctaacatata
      481 cattatttca aaatcagtaa ttgccacatc actagttcct atcttaatag gaatatcctc
      541 ttttgttgcg agtcttttag ttccgttggt tactaaaagg ttagcgctaa atagggtttt
      601 atctttatct caatttggaa agactatatt attggcgata ctggtaggaa tgtttaccgt
      661 aatgcaatcc gtagcgcctt tggtgaccta tctatttgtt gttgcaattt ccatactaga
      721 tggttttgca gcacccgttt cctatgctat tgtgccacgc tatgcgaccg atttgggtaa
      781 ggctaattca gccttatcaa tgactggtga agctgttcaa ttgataggtt ggggattagg
      841 tggactcttg tttgcaacaa ttggtctgtt acctaccacg tgtatcaatt tagtcttgta
      901 tatcatttct agctttctga tgttatttct tcctaacgct gaagtggagg tgttagagtc
      961 agaaactaat cttgaaattt tgctcaaagg ttggaagtta gttgctagaa atcctagatt
     1021 aagacttttt gtatcagcaa atttattgga aattttttca aatacgattt gggtttcttc
     1081 cattatactt gtttttgtaa cggagttatt aaataaaacg gaaagttact ggggatattc
     1141 taatacagca tactctattg gtattataat tagtggctta attgctttta ggctatctga
     1201 aaagttcctt gctgctaaat gggaaagtat tctttttcct cttgtagcta tggaaatagt
     1261 gacactgact attctatatt ttccaaacgc acagatgttt ttattatttt cagctttagt
     1321 tggtatgtta tcgcaactaa aagaagttcc tgaaagtgta tttcttcagg aaacagtaga
     1381 ggaaaatcat ttagttaatg tctattccgt tcttgaagtg atttctacat tagcattttc
     1441 agtatttgtt ttgctaatga gctatattac tgagagtttt ggtattagca tcagtttttg
     1501 gctatcagcc atttgtctga tgatagaagc tattttaatt tatatcagac gagattattt
     1561 taaatgagag agtggttaaa aagtctttaa aaatcagaaa agcgcatagt atcaggtgtt
     1621 gaaaaaactt gatattgtgt gttttattat gggaagattt acttcattgt ctcctgaaat
     1681 ggagttttga agcaggatca ctctatttga aaatatacat agaaaaactg tataatcctt
     1741 aaaataagct gaaatctatt ggaaacatac ggtaaacaat tgaaaagata ctatataaag
     1801 tggtacaatg gtagaaaaat aagctagtaa gaatcactct tattttagcg aaaaattaca
     1861 gaaatgaatg gtttttcttg atgaaacaga gaaaagaatt gtacctcttt cttggtcgga
     1921 cagccttgta ttttcttatc tttctagggc tgctttactt ctttagctat cttggtcagg
     1981 gtcaaggaag ctttatctat aatgaatttt aacttgaagg agaatcccta ttgtgtcaaa
     2041 taaaccaata gcagatatga ttgaaaccat tgagcatttt gctcagacac agcctagcta
     2101 tcctgtctat aatgttttgg ggcaggaaca cacttatggc gatttaaagg ctgattcgga
     2161 tagtttggct gcagtcattg accaactagg cttgcctgag aagtctcctg tggttgtttt
     2221 tggtggccaa gaatatgaaa tgttggcaac ctttgtagcg ctgactaagt caggtcatgc
     2281 ctacattcca attgatagcc attcggcctt ggagcgagtt tcagctattt tagaagtagc
     2341 agagccaagc ttgattattg ccatttcagc ctttcccttg gagcaggttt ctacaccaat
     2401 gataaatcta gctcaggttc aagaagcctt tgcccaaggg aataactatg aaatcacgca
     2461 tccagtcaag ggagatgata attactacat tatctttact tctggtacga ctggtaagcc
     2521 taagggagtg cagatttcac atgataatct cctcagcttt acaaactgga tgattacgga
     2581 taaggaattt gcgacaccga gtcgtccgca aatgctggca cagccacctt attcttttga
     2641 cttgtctgtc atgtattggg caccgacctt ggcacttggt ggtacgcttt tcactcttcc
     2701 ttcagtcatc actcaggact ttaagcaact ctttgcggct atcttttcat tgccaatcgc
     2761 tatctggaca tcaacaccat cctttgcaga tatggccatg ttgtctgaat acttcaacag
     2821 tgagaaaatg cctggaatca cgcatttcta ctttgatggt gaagaattga cggtcaaaac
     2881 agctcaaaaa ctgcgcgagc gtttcccaaa tgcccgtatc atcaatgctt acggcccaac
     2941 agaagcgaca gtagctctgt cagcagttgc cgtgacagac gagatgttag cgactctcaa
     3001 acgcctacca atcggctata ccaaggctga ttctccaacc tttatcattg acgaggaagg
     3061 aaataaactg ccaaatggtg agcagggaga aatcattgtt tctgggccag ctgtttcaaa
     3121 aggttatatg aacaatcctg aaaaaacagc agaagccttc tttgagtttg aagatctgcc
     3181 agcctatcac acaggcgatg tgggaaccat gacagatgag ggcttgcttc tctacggcgg
     3241 acgcatggac ttccagatta agtttaacgg ttaccgcatt gagttagaag atgtctctca
     3301 aaacctcaac aagtctcgct ttatcgaatc tgctgtcgca gtaccgcgct ataacaagga
     3361 ccacaaggta caaaatctat tggcttatgt catcttaaaa gacggtgttc gtgagcagtt
     3421 tgagcgagat atcgatatta ccaaggccat caaggaagac ctgacagaca tcatgatgtc
     3481 ctatatgatg ccatctaaat tcctttaccg agacagtttg ccactaactc caaatggaaa
     3541 gattgacatc aaaggattga ttaacgaggt gaataagaga tgatggagtt ttttcaacag
     3601 cttcctcatt tagagccata tggcaatcct cagtattttg tttatgtgat tgctgcaacc
     3661 ttgcccatct ttataggtct ctttttcaag aaacgctttg cctggtatga agtgttggta
     3721 agtctcttct ttattgtcac catgttggtg ggtggaaaga ccaatcaact agctgccttg
     3781 ggtatttacc tttgctggga aatattgctc ctgcttttct acaagcatta tcgaaaaagc
     3841 aaggatggca agtgggtctt ctacttagtt agttttctgt ccctacttcc gattatcttt
     3901 gtcaaggtgc aaccagctat caatggaacg cagtctttgc ttgggttctt gggaatttct
     3961 tacctgacct ttcgttcggt tggaattgtc atcgatctga gagatggagt gattaaggat
     4021 tttaccctct gggaattcct ccgtttcctt ctcttcatgc caactttctc gagtggtcca
     4081 atcgatcgct ttaagcgatt taatgaaaat tatcaggcta ttcctgagcg agatgagttg
     4141 atggatatgc tggatgaatc tgtccgctat atcatgtggg gctttttgta taagtttatc
     4201 ctagctcatg ttttaggaga gaccttacta cctcctctga agaatttagc cttgcagtca
     4261 ggtggcttct ttaatctcta tgccttggca gttatgtata cttttggtct ggaactcttc
     4321 tttgactttg caggttattc tatgtttgct ttggccatct caaacttgat gggaatccgt
     4381 agccctatca actttaacaa gcccttttta tcaagggatt taaaggagtt ttggaatcgc
     4441 tggcatatga gtctgtcctt ctggttccgt gactttgtct ttatgcgaat ggtgatggtg
     4501 ttaaccagaa agaaagtctt taaaaatcgt aatgtaacct caagcatggc ctacattgta
     4561 aatatgctga ttatgggatt ttggcatggt gtgacctggt actatatcgc ctatggactc
     4621 tttcatggac taggcttggt catcaatgat gcctgggttc gcaagaaaaa aacgctcaat
     4681 aaggaacgga aaaaagcagg gaaggctgcc ctacctgaga atcgctggat tcagttgctt
     4741 ggcatggttg tcactttcca tgttgtcatg ttgtcattct taatcttttc tggattcttg
     4801 aataatctat ggtttaaaaa ataaaaggaa ataaaatatg gatatcaaat cagaagttat
     4861 tgaaattatt gatgagttgt ttatggaaga tgtttctgac atgatggatg aagatctttt
     4921 tgatgcaggt gtcttggata gtatgggaac ggttgagttg attgtggaga ttgagaaccg
     4981 ttttgacatt cgtgtccctg taacagagtt tggtcgtgac gactggaata cagctaataa
     5041 aatcatagct ggtattgtgg agctacaaaa tgcttaaacg cttatggatg atcttcggac
     5101 cggtcttgat cgctggtttg ttggtttttc tgctcatttt cttttatcct actgagatgc
     5161 atcataatct aggagctgaa aagcgttcag cagtggctac tactatcgat agttttaagg
     5221 agcgaagtca aaaagtcaga gcactatctg atccaaatgt gcgttttgtt cccttctttg
     5281 gctctagtga atggcttcgt tttgacggtg ctcatcctgc ggtattagct gagaaataca
     5341 atcgttccta ccgtccttat cttttaggac aggggggagc tgcatcgctt aaccaatatt
     5401 ttggaatgca acagatgtta ccacagctgg agaataaaca agttgtgtat gttatctcac
     5461 ctcagtggtt cagtaaaaat ggctatgatc cagcagcctt ccagcagtat tttaatggag
     5521 accagttgac tagttttctg aaacatcaat ctggggatca ggctagtcaa tatgcagcga
     5581 ctcgcttact gcaacagttc ccaaacgtag ctatgaagga cctggttcag aagttggcaa
     5641 gtaaagaaga attgtcgaca gcagacaatg aaatgattga attattggct cgttttaatg
     5701 aacgccaagc ttcctttttt ggtcagtttt cggttagagg ctatgttaac tacgataagc
     5761 atgtagctaa gtatttaaaa atcttgccag accagttttc ttatcaggca atagaagatg
     5821 ttgtcaaagc agatgctgaa aaaaatactt ccaataatga gatgggaatg gaaaattatt
     5881 tctataatga gcagatcaag aaggatttga agaaattaaa ggattctcag aaaagcttta
     5941 cctatctcaa gtcgccagag tataatgact tgcagttggt tttaacacag ttttctaaat
     6001 ctaaggtaaa cccgattttt atcattccac ctgttaataa aaaatggatg aactatgctg
     6061 gtctacgaga ggatatgtac caacaaacgg tgcagaagat tcgctaccag ttagaaagtc
     6121 aaggttttac caatatagca gatttttcta aggacggcgg ggagcctttc tttatgaagg
     6181 acaccattca ccttggttgg ttgggttggt tggcttttga caaggcagtt gatcctttcc
     6241 tatccaatcc cacaccagct ccgacttacc atctgaatga gcgctttttc agcaaagatt
     6301 gggcgactta tgatggagat gtcaaagaat ttcaatagat aatataatat agaagagttc
     6361 aagaatgaaa ctagttttca cgttttttct tgactctttt tttgcttgtg atataattaa
     6421 ctggtaaaca aaatgtcaaa gaatagtatt tttctcttaa aaaagagaag tctaaaagga
     6481 aaggagtcag atatgagaca gctagcaaag gatatcaatg cttttttgaa tgaggtgatt
     6541 ttgcaggcgg aaaatcagca tgaaatccta ataggtcatt gcactagcga ggtggccctg
     6601 accaatactc aggagcatat ccttatgctc ttgtcagagg aatctttaac aaattcagaa
     6661 ttggcccgtc gtctcaatgt cagtcaggcg gcagttacca aggccattaa gtctttggtc
     6721 aaggaaggga tgttggaaac atctaaagat tctaaagatg cgcgtgtgat tttttatcag
     6781 ttgactgact tggctcgtcc aatcgctgag gagcaccacc atcaccatga gcatacactt
     6841 ttaacctatg aacaagtggc gactcagttt actccaaatg aacaaaaagt gattcagcgg
     6901 tttttgactg ctttagtagg agaaatcaaa taatgagata tattacggta gaggatttgt
     6961 ccttctatta tgataaggag cctgttcttg aacatatcaa ttatagtgtt gatagtgggg
     7021 aatttgttac cttgactggg gaaaatggag cggctaagac gacgctcatc aaggccagtc
     7081 ttggaattct gcaaccacgc attggaaagg tggctatttc aaagacaaat acgcaaggta
     7141 acaaattgag aatagcctat cttcctcaac aaattgccag ttttaatgct ggttttccaa
     7201 gtacggtcta tgaatttgtc aagtcgggtc gctatccgag aaaaggctgg ttccgtcgtt
     7261 tgaatgctca tgatgaggag catatcaagg ccagtctgga ctcagttggc atgtgggaac
     7321 atcgagacaa acgcttgggg tctctatctg ggggacaaaa gcagcgagcg gtaattgcgc
     7381 gtatgtttgc ttctgaccct gatgtgttta tcctagacga gccgacaacg gggatggatg
     7441 caggaagtaa aaatgaattt tacgaactca tgcaccacag cgcccatcat catggcaagg
     7501 ctgttttgat gattacccat gaccctgaag aagttaagga ttatgcggat cgcaatattc
     7561 atctagtccg taaccaagac tcgccatggc gttgtttcaa cgttcatgag aatggccagg
     7621 aggtgagcca tgcttagttt attatcttac gactttatac aacgcgcctt tttggcggtt
     7681 attgctatga gtcttttctc accggtattg ggaaccttcc tcatcttgcg tcgtcagagt
     7741 ttgatgagtg atacccttag ccacgtctca ctttcaggtg tagcctttgg tctggttttg
     7801 gggatttctc caactgtttc tactattgcc attgtcttga ttgcggcggt ctttctggag
     7861 tatctccgta cggtttacaa gagctttatg gaaatcggga cagctatcct catgtcaaca
     7921 ggtctggctg tttctctgat tgtcatgagc aagggtaaaa gctcgagttc aatgagtttg
     7981 gaccaatatc tctttggttc gatcgtgact atcagtgaag aacaggtcat ttccctcttt
     8041 gtcattgcgg cggttgtttt gattttgacc tttctctttc ttcgtcctat gtatatctta
     8101 acttttgacg aagatacggc ctttgtggat ggcttgccag ttcgtaccat gtccattctt
     8161 tttaacatgg tgacaggggt ggctattgcc cttatgattc ctgcagcagg agctcttctg
     8221 gtatcgacca ttatggtctt gccagctagt attgccctgc gtctggggaa aaactttaaa
     8281 tcggttatgc tgcttgccag tgcgattggc tttttgggaa tggtagcagg actttacatt
     8341 tcctactatg cagaaacacc tgcaagtgca agtattacca ttatttttgt aactgtcttt
     8401 atactaatca gtttagtaag acgttttatc aaataggaga cgaacgtgaa aaaaattagc
     8461 ttattactag ccagtctatg tgccttgttt ttagtggctt gttccaatca aaaacaggca
     8521 gatggtaaac tcaatatcgt gacaaccttt taccctgtct atgaatttac caagcaagtc
     8581 gcaggagata cggctaatgt agaactccta atcggtgctg ggacagaacc tcatgaatac
     8641 gaaccatctg ccaaggcagt tgccaaaatc caagatgcag ataccttcgt ttatgaaaat
     8701 gaaaacatgg aaacatgggt acctaaattg ctagatacct tggataagaa aaaagtgaaa
     8761 accatcaagg cgacaggcga tatgttgctc ttgccaggtg gcaaggaaga agagggagac
     8821 catgaccatg gagaagaagg tcatcaccat gagtttgacc cccatgtttg gttatcacca
     8881 gttcgtgcca ttaaactagt agagcacatc cgcgacagct tgtcagcaga ttatcctgat
     8941 aaaaaagaga cctttgagaa gaatgcagct gcctatatcg aaaaattgca agccttggat
     9001 aaggcttacg cagaaggctt gtctcaagca aaacaaaaga gctttgtgac tcaacacgca
     9061 gcctttaact atcttgcctt ggactatgga ctcaaacaag tcgcaatctc aggactttct
     9121 ccagatgcag agccatcagc tgctcgcttg gcagaattga cagagtatgt caagaaaaat
     9181 aaaatcgcct atatctattt tgaagaaaat gcctcacaag cccttgctaa cacactttca
     9241 aaagaagcag gtgtcaaaac tgatgtcctc aatcctttag aaagtctgac agaagaggac
     9301 accaaggctg gagaaaacta catttccgtg atggagaaaa acctcaaggc tttgaaacaa
     9361 acaacagacc aagaaggccc agcaatcgaa cctgaaaagg cagaggatac caagacagtc
     9421 caaaatggtt acttcgagga tgcagctgtc aaggaccgca ccttgagtga ctatgcaggt
     9481 aactggcaat cagtttatcc tttccttgaa gacggcacgt ttgaccaagt ctttgactac
     9541 aaggctaagt tgactggtaa gatgacccag gctgagtaca aggcttacta tacaaaaggc
     9601 tatcagacag atgtgactaa gattaacatt actgataata ctatggaatt tgttcaaggt
     9661 ggacaaagca agaaatacac ttacaagtat gtcggtaaga aaattttgac ttacaagaaa
     9721 ggcaatcgtg gcgtgcgttt cctctttgaa gccacagatg ctgacgctgg acaattcaag
     9781 tatgttcagt ttagtgacca caatgttgcc ccagttaagg cagaacattt ccatatcttc
     9841 tttggaggca caagccaaga agccctcttt gaagaaatgg acaactggcc aacctactac
     9901 ccagataacc tatctggcca agaaatcgcc caagaaatgt tggcgcattg atgagaaatc
     9961 gactagttca taagagctag tcggtttttt gatactagat ggctaacttc attatagacc
    10021 tttcagaact ttaaacaata aataaaactc ttgaaatatc tgggcttata tgctagaatg
    10081 tagttagtgt ataaggttca ttagaacacc aaagccccta actagtgcca tagttaaggg
    10141 cttttttgac gtatcttagc gatttaacgg gtgtttatag gctagtcatt cggtctactt
    10201 cttaccatct agccatttgc agacgaaata caagataatt ccagctataa catctgctat
    10261 aatagtaaga gcaagctctg gtataaggct cattagtttc acctcctctc acgaaccgta
    10321 aggaacgtaa tcggtaaccg atgacaaaaa tagtatatca cactacattc aggtcatcaa
    10381 ccttacttaa tgcttgaact attgtagatt atatgctaga atgtagttaa ttgataaggt
    10441 tcattagaac accaaagccc ctaactatgg ccgtagttaa gggctttttt gacgtatctt
    10501 agcgatttaa cgggtgttta taggctagtc agtcggtcta ctccttacta tctagccatc
    10561 tgcagacgaa atacaagata attccagcta taacatccgc tataatagta agagcgagct
    10621 ctatgataag gctcattagt ttcacctcct ctcacgaacc cataggaacg taatcggtaa
    10681 ccgatgacaa aaatagtata ccacaataca tttagatcat caaggtcact taattcttga
    10741 aatatcagat ctaagagaaa aatctttaaa atcagaaaaa cgcataatat caggtgtgca
    10801 aaaacttgat actatgcgtt ttattgtggg aaggtttact ccattttctc ctgaaattga
    10861 gtttttgtcc agcctctgtt tttagggttg ctaagaaaat aatgtcatgt ggtgaatatt
    10921 tgtaaatcag tcagcagaca gaacgatact cttcgaaaat ctcttcacat catgtcagct
    10981 tcgtctttcc gtatatatgt gactgacttc atcagttcta tctacaacct caaaacagtg
    11041 tttcgagctg acttgatcaa ttttcaaatc tgtactttga gcaagctgag actagcttcc
    11101 tatttgattt tcattgaata tcagaaaccc attctccatc aaataattcg actgcgtcta
    11161 ataatttttg atctggcacg gtgtctgaaa taaaggttgt gtatttggag aggggattaa
    11221 ttttaaaaaa tccagtcttg taaaatttag aactatcaat cagtaagatg gtttcatggg
    11281 ctttgtcaat aatattcttt tttgaaatag cttggctgag agaagcttca taaacatatt
    11341 ggtcatcaat acctcttgct gaacaaaatg ctaaatcgat attaaaatga tctaataaag
    11401 aattttcctt atcatagttg accacggaac aggattgatg tttgacctcg ccagatgtga
    11461 taaagatttt ggagctatct ttaacagttt cagatagggt ttgtgcagta tgtaaaccat
    11521 ttgtaaaaat aatcaaatta tcaagttcag aaagataggg acagagttcg tagacagtag
    11581 tactagaatc tagatagata cacataccag accgaataaa gtctttagcg agactagcga
    11641 ttagtctttt ttgcctagta ctttctcctt cacgtatttg atgagaaagt tcaattgtgt
    11701 tcatagagga cagggtcacg tatccgtgct ttcttttgat aagaccttga ttttctaaga
    11761 aaattaaatc acgacgtaag gtacttgtgc tggagaaagt gatttctgcc agctctttta
    11821 cggcaattct ttttttcttt ttgataattt caatcaattc aagtacacgt tcatctttta
    11881 tcataagctc ctcctaattt atcatttcaa ctatattata gcacaaattg gaggaatttg
    11941 aattattttt atgaatattg ggttaacatt tgaacattat tcaagtaagc gttcacatat
    12001 tgaaaaaata aaacgtgggg attataataa agttaatcaa ggacgaagag agaagaaaaa
    12061 tggaagcggt tttagcaata gatttaggtg cgacttctgg aagagcaatc gttggttacc
    12121 tttctgaaaa taaactagta atggaagaaa taaatcgctt ttctaatcta cctattagag
    12181 taaaagggca tttatcttgg gatattgact ttctactagc taaaattctt gaaagtatcc
    12241 gcttggctaa tactagttac aagattttat ctatcggtat tgacacatgg ggagttgatt
    12301 ttggactgat tgataatgaa ggtaagctgt tattacaacc tgttcattat cgtgatgaaa
    12361 gaacaaaggg agtgttaaag gaaatatctg aaatgactga attagaaaaa ctgtattcag
    12421 agacaggaaa tcagattatg gagataaata ccttgtttca actctttaag gcacgtcaag
    12481 aatctcctga ctctttctat aagaccaata agattctttt aatgccagat ttgtttaatt
    12541 atctcttgac aggtaagttt gctacagaaa aaagcattgc ttcaacaact caattatttg
    12601 atcctaggag tcaaaattgg aatcagaata tcttaaaact atttgaattg gattcatctt
    12661 tacttcctga aattgtttca gagggaaatg ttcttggaag gataaaagag gagtatggtt
    12721 taggcgatat tcctgttgtg aatgtttgta gtcatgatac agcaagcgcg attgtctcag
    12781 tacctaagac agaaggtagt ttatttattt catcaggtac ttggtctttg gttggagtgg
    12841 aacttacttc accgattctt actaccgaat ccttcagtta tggatttaca aatgaagtcg
    12901 gtaaagatgg agtgattaca tttctgaaga attgtacagg gttgtggatc atagaggaac
    12961 taagacgttc atttgaacga agagggaaag cctattcttt tgatgatatt aggacaatgg
    13021 tggagaaaga aaaagaaaat cttcctctga ttgatactga atcaactgaa tttgcaacag
    13081 aatctgatat gcacaagact ttgacagaat atctagctta tcatcatgaa actagagagt
    13141 ggacagatgg acaactattt aagattgttt atgaaagcct agctgaaacg tataggaaag
    13201 cgatagagtt actagaagaa ttaactcata aggtttataa gaggatatat gtgattggag
    13261 gaggtgctag agccagttac tttaaccaaa tgattgctga tagaactggt aaagaggttc
    13321 ttacaggttt gactgaggct acagctgtgg ggaatattgt tgtgcagctc atagctatgg
    13381 gacaattaaa agggatggaa gaggctcacc atgttattga ggagtttcta caattagaga
    13441 gttattactc ccaaaagaat taaaaagatt gagagtttgt aaatttgcct ccctccccct
    13501 tcttagcttt tgtgcaggaa ggggggataa ttggtgaatt gaaaaatatt tagtgttttg
    13561 atatgaggag gacaaggatg tcagatgtaa aacaagaatt aattaaatat ggtaagaagc
    13621 tagtagaaac agatttgacg aaaggaacag gtgggaatct cagcgttttc gatcgtgaaa
    13681 aacaattgat ggcaattacc ccgtcgggta ttgatttctt tgaaatcaaa gaatccgata
    13741 ttgtagtgat ggatattaat ggaaatgttg tagagggaga acgcttgcca tctagcgaat
    13801 ggtatatgca tttgattcaa tatcaaactc gtgatgatat cgatgcaatt atccatgctc
    13861 atacaactta tgcaacagta ttagcttgtc tcagagaacc acttccagcg agtcattata
    13921 tgattgcagt ggcagggaaa gatgttcggg tagctgagta tgcaacatat ggcacgaaag
    13981 aattggctgt gaatgcagct aaagcaatgg aaggtcgtag agcagtttta ctagcgaatc
    14041 atggaatttt agcaggtgca caaaatttat tgaatgcatt taatattgtt gaagaagttg
    14101 aatattgtgc aaaaatttat tgtttagcta agaattttgg agagccagta gttcttcctg
    14161 atgaggagat ggaattgatg gcagaaaaat ttaaaacata cggtcagaga aaatagggag
    14221 gatattaatg ttaaaacata taccgaaaaa tatttctcca gatttattga agactttaat
    14281 ggaaatggga catggagatg aaatagtatt agctgacgcg aattatcctt ctgcctcatg
    14341 tgcaaataag ctaattcgtt gtgatggtgt aaatattcca gaattattag attccattct
    14401 gtatttaatg ccattagata gttacgtcga tagttcaatt cagtttatga acgttgtttc
    14461 gggtgatgat attcctaaga tatggggtac ctatagacag atgattgaag gtcatggtac
    14521 agatcttaaa acgattactt atcttagaag agaagacttt tatgaacgta gtaagaaagc
    14581 ttatgctatt gttgctacag gagaaacttc actttatgct aatattatcc ttaagaaagg
    14641 agtagttgtt gaaagagaaa atgttcaata gaggaatttt agttgccagt catggtaatt
    14701 ttgctagcgg agctctcatg accgcagaaa tgtttgttgg tgagacaaca aatgatagag
    14761 ttaggacatt aggtttgatg cctggagaga atattgtaga gtttgagcat tattttaaaa
    14821 atcaagtgga tgaactgtta gactcaaatc aagaggttat cgttttgact gacttgattg
    14881 gaggaagtcc taataatgtg gctttgtcac ggtttttaaa tttggattca gttgatattg
    14941 taacagggtt taatatccct ctcctagtgg aattaatatc aagttatgat tcaaaaatca
    15001 atttagaaga aattgttcac aatgctcaaa atagtttgtt taatgttaaa caacaactta
    15061 acgtagagga ggaagaagat ttatgtctat agagtttgtt cgtattgatg accgtctggt
    15121 acatggtcaa gttgtcacta cgtggctaaa aaagtatgat attgagcaag ttatcattgt
    15181 taatgatcgc atctcagaag ataaaacacg acaatctatt ttaaagattt ctgcaccggt
    15241 aggtttaaaa attgttttct ttagtgtaaa acggtttgtg gaagttttaa actctgtgcc
    15301 aataaaaaag agaacaatgc tgatatatac aaatccaaaa gatgtgtatg attctattga
    15361 aggaaattta aaattggagt acctcaatgt aggacagatg agtaaaacgg aggaaaatga
    15421 aaaggtaacg ggaggtgtag ctctaggtga agaagacaaa tattatttta agaaaatagt
    15481 tgataaggga acgagagttg aaattcaaat ggttcctaat gataaagtta caatgttgga
    15541 aaaattttta taaaaataat ttaaggaggt acagtatatg ctattcacac aagcattact
    15601 ggtgacatta gttgggatta ttgccactat tgactataat ggaccgttat ttatgattca
    15661 ccgtccgtta gttacaagtg caatggttgg cttagtatta ggagatttca cccaaggtgt
    15721 tcttattggt tcagctcttg aattaacttg gctcggtgta acaggtattg gaggttatac
    15781 tccaccagat actatttcag gtgcgattat tggtactgca tttggtattt tatctggtca
    15841 aggagaaact gctggtatcg ctatagcagt tccaattgca gttgctaccc aacagttgga
    15901 tgttcttgca aaaactttag atgtttattt tgtgaaaaaa gctgataatg atgctaaaaa
    15961 cggagattat tcaaagatcg gtttttatca ttattcaagt ttggttttaa tcacgttatt
    16021 taaaattgta ccaattttcc tagctattat gcttggaggg gaatatgtgg cagacttgtt
    16081 tgctaaggtt ccaccaatcg ttatgcaggg acttaactct gcaggtgctt tactaccttc
    16141 aattggtttt ggtatgcttt taaatatgat gctcaagaaa aatatgtggg tattcttgtt
    16201 gattggattc atttgttctg tgtatggagg aatgtcaacc attgggatct cactagttgg
    16261 tattgcggta gcatacttct acgatatgat tggaagcaaa ccacaagaaa caacttcaag
    16321 tagtgatgtt gaggaggatc ttgatctatg atgaataata aagtaactaa agttgaactt
    16381 aaaaaagttt tcaaacgaag ttttatgtat ggttcttcat ggaactatga gagaatgcag
    16441 aacctaggtt ttctatatac aattcttcca gtattgaaaa aactataccc agacaaagat
    16501 tcagcttctc ctgcaatgaa acgtcacctt gagtttttca atactcatca aacagcggca
    16561 ccatttattc ttggagttac ttccgctatg gaagaacaag aaggaaatga aggtgcagct
    16621 tcaattactg gtattaaagt tggcttgatg gggccactgg ctggtctagg agatagtttg
    16681 ttctggctga cactagttcc tatctgtttt agtattggtg cgtcttattc taaagacggc
    16741 ggtgctttag gtatcttt
//
LOCUS       DAVHEW010000040        16736 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVHEW010000040 DAVHEW010000000
VERSION     DAVHEW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.040"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            138..686
                     /locus_tag="VVL53_001186"
     CDS             138..686
                     /locus_tag="VVL53_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU4183365.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            796..1695
                     /locus_tag="VVL53_001187"
     CDS             796..1695
                     /locus_tag="VVL53_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU4183366.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1720..2994)
                     /gene="serS"
                     /locus_tag="VVL53_001188"
     CDS             complement(1720..2994)
                     /gene="serS"
                     /locus_tag="VVL53_001188"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU4183367.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3207..3578)
                     /locus_tag="VVL53_001189"
     CDS             complement(3207..3578)
                     /locus_tag="VVL53_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU4183368.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3581..4945)
                     /locus_tag="VVL53_001190"
     CDS             complement(3581..4945)
                     /locus_tag="VVL53_001190"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU4183369.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKNQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5270..6055
                     /locus_tag="VVL53_001191"
     CDS             5270..6055
                     /locus_tag="VVL53_001191"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU4183370.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6149..6583
                     /locus_tag="VVL53_001192"
     CDS             6149..6583
                     /locus_tag="VVL53_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4183371.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6583..7557
                     /locus_tag="VVL53_001193"
     CDS             6583..7557
                     /locus_tag="VVL53_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU4183372.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7617..7841
                     /locus_tag="VVL53_001194"
     CDS             7617..7841
                     /locus_tag="VVL53_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4183373.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7960..8934
                     /gene="fabK"
                     /locus_tag="VVL53_001195"
     CDS             7960..8934
                     /gene="fabK"
                     /locus_tag="VVL53_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU4183374.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            8927..9847
                     /gene="fabD"
                     /locus_tag="VVL53_001196"
     CDS             8927..9847
                     /gene="fabD"
                     /locus_tag="VVL53_001196"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU4183375.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9881..10612
                     /gene="fabG"
                     /locus_tag="VVL53_001197"
     CDS             9881..10612
                     /gene="fabG"
                     /locus_tag="VVL53_001197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU4183376.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            10634..11869
                     /gene="fabF"
                     /locus_tag="VVL53_001198"
     CDS             10634..11869
                     /gene="fabF"
                     /locus_tag="VVL53_001198"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU4183377.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11872..12357
                     /locus_tag="VVL53_001199"
     CDS             11872..12357
                     /locus_tag="VVL53_001199"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU4183378.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12354..12776
                     /gene="fabZ"
                     /locus_tag="VVL53_001200"
     CDS             12354..12776
                     /gene="fabZ"
                     /locus_tag="VVL53_001200"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU4183379.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVVIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            12788..14155
                     /gene="accC"
                     /locus_tag="VVL53_001201"
     CDS             12788..14155
                     /gene="accC"
                     /locus_tag="VVL53_001201"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU4183380.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14192..15058
                     /locus_tag="VVL53_001202"
     CDS             14192..15058
                     /locus_tag="VVL53_001202"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU4183381.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15055..15822
                     /locus_tag="VVL53_001203"
     CDS             15055..15822
                     /locus_tag="VVL53_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU4183382.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            15981..16166
                     /gene="briC"
                     /locus_tag="VVL53_001204"
     CDS             15981..16166
                     /gene="briC"
                     /locus_tag="VVL53_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU4183383.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            16184..16657
                     /locus_tag="VVL53_001205"
     CDS             16184..16657
                     /locus_tag="VVL53_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183384.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
BASE COUNT         4797 a         3155 c         3880 g         4904 t
ORIGIN      
        1 attggaagtg tgataaacta atataacaac gaaaatctta tgagagaata aagctttatc
       61 ttagataaac ttaggaatca ggattttata agattttcca ataatattag tgtcaataat
      121 aagaaaagag gtatcttatg acaacattta ctatccatac agtagagtca gcaccagcag
      181 aagtgaaaga aattcttgaa acagtagaaa aagacaacaa tggctatatt cccaacctaa
      241 tcggtctctt ggccaatgcc ccgactgttt tagaagccta ccaaattgtc tcatctatcc
      301 accgtcgcaa cagcctgaca cccgttgagc gtgaagtggt gcaaatcacg gcagccgtga
      361 ccaatggttg tgccttctgt gtcgcaggtc acacagcctt ttccatcaaa caaatccaga
      421 tgaatgatga cttgattcaa gctcttcgca atcgtactcc aattgaaaca gatcctaaat
      481 tggataccct agctaagttt accttggcag ttatcaatac caagggtcgt gtaggagatg
      541 aagccttgtc tgagttttta gaagctggct acactcaaca aaatgccttg gatgtggttt
      601 ttggtgtcag tctagcaatc ctctgtaact atgccaacaa cttagctaat acaccaatta
      661 atccagaatt gcaaccttat gcctaattca tatctgagta aaatgaagtc tgaaataagc
      721 ggacttcgtt tttttaaatc ctcatttaag cgcttttatg ttatactgaa actaacatga
      781 ttattggagg ttaggatgaa aaaactcccc ttagtatttt caggttgttt gctaggtttg
      841 gcaggagctg gaaatcttat tttagatacg ttgccggttc tatcccatct ttttagtctg
      901 actggtttgg ttttatggat ttactttcta attctgcatc tctttaattg gaaagaaacc
      961 aagcaagaat tgaccaagcc ccctcttttg tcaggaatgg caacctttcc tatggctggg
     1021 atgattttat cgacctatgt ctttcgcgta ttctcttatc ttcctttggt agcacaaggg
     1081 atttggtggt tttcatttct cttggatttg accttgattg ctggttttac catcaagttt
     1141 gcttgtccag ggcggagggt tcatgccact ccaagctgga cggttctcta tgtggggata
     1201 gcagtggctg ccttgaccta tcctctggta ggtattatcg aaattgccta tgcgaccttg
     1261 agttttggtt ttctcttgac cttctatctc tatcccctta tttatagcga tttaaagaaa
     1321 catccactcc cactagcctt gcttggacaa gaaggaatct actgtgctcc tttctctcta
     1381 ctcttggctt ctctagttcg agtaggagga accagcctgc cgacttgggt cttgattgtc
     1441 atgattttgg cttctcaatc cttctttttc tttgttttaa ctcgtctgcc caacatttta
     1501 aaacaaggtt ttcaaccagc cttctcagcc ctcaccttcc caaccattat cacagcgacc
     1561 tcgctcaaga tggctcaggg aattttgaaa cttccatttc tggattacct ggtattggct
     1621 gaaaccatta tatgcctaac tattttattc tttgtactag gtgcttatct gatttggtta
     1681 cgaaaaaagg tctagctaga aatagctaaa ccttattttt tatggtttga tgacttcagc
     1741 tccacccatg tatggacgaa gtgcttctgg gatggtcaca gaaccatctt cattttggta
     1801 attttcaaga attgcagcca ctgtacgtcc aactgcaagt ccagaaccgt tcaaggtatg
     1861 aaggagtttc accttgccat ctgcttcatc acggtaacgg atttgggcac gacgggcttg
     1921 gaaatcttct gtgtttgaac agcttgagat ttcacggtaa ttgttttgtg ctggaatcca
     1981 cacttccaag tcgtaagtct tcgcagctga gaagcccata tctccagtag agagagcaac
     2041 gacacggtat ggaaggttga gtttttgaag aatgttttca gcgttggctg tcattttttc
     2101 caattcttcg taagattctt ctggtttggc aaatttgacc atttcaacct tgtggaattg
     2161 gtgcaaacgg atcaagccac gcgtatcacg accggcagaa ccagcctcag aacggaatga
     2221 cggactcatg gcagtgaagt agattggaag atctttgccg tctaagattt catcacggta
     2281 gtagtttgtc agaggaactt cagcagttgg aatcaagaca aagttggtat cgctgagttc
     2341 aaaagtatct tccttaaatt ttggatactg accagtacca aacatagaat catggttgac
     2401 tatgtaaggt gtgatgactt cagtatagcc ttcttttcca tgttcatcca acataaagtt
     2461 gtagatagca cgttccaaac gagcaccgag gcctttatag aagaggaagc gagcgcctgt
     2521 taccttacca ccgcgttccc agtcaaggat accaaggtct tcaccgagat cccagtgagc
     2581 tttaggttcg aagtcaaact cgcgtggagt accccaacgg cgaacttcca cattgtcgtc
     2641 ttcgtcagcc ccaacaggaa cgctgtcagc tgggatattt ggaagagtcg ttgtaaattc
     2701 tgtcaattta gcatcgattt ctgccaattc agcatccaag gctttaacct cagcagatag
     2761 attttgcatg gcagcaatct tgtcatctgt attttccttg ttgcgcttag cttgggcaat
     2821 ctcagcagaa actgtgttac gttctgcttt gagagtttca accttgacca agatgttacg
     2881 acgtttagca tcgatttctt tcatttcatt caagacagca gcatctacac cacgtgtagc
     2941 taatttttct gcgacagctt caaaatctgt acgaatacgt ttgatatcta acataagaac
     3001 tcctttatga aaaaagcacc ctgacaaaac gttggagtgg cagggccacg gttccatcca
     3061 acttcacagg tgtgcacttg attgtgtatg taattgtcac taacggtaga atttcaccta
     3121 tccctcctat ctgctcgcag tacccgcaga ctttctgaaa gaagaagata acctacttat
     3181 ccgttgctat gattatacta aagtttctac ttttttgcaa atagattttt aaatttttgg
     3241 ctaattgtct gaatcagggt cggaagtttg acgaccttgt cattgcctag tttttcgcgt
     3301 gcaattttga gaatggcacc tgagtctttt gaagcaaaga ggaattttcc tttgtctgta
     3361 aagacttcga agtggcggct gattttgcgt ccagtgacat tggctccaat ctgattgata
     3421 tggctccaag gaatctggat aaattgttcg acattgacat ctgggtaaaa ttccaaagcc
     3481 tgatctccga caaggaattt cccaactttc ccagcgatag agaggtagga agtgcctgtc
     3541 gtactgagga gtactgtttt gttaagtgat tgggccatgc ttagtcttcc ttactttctc
     3601 caaaaaaggc attgtagagg gctttaattg ctgctttctc ttggtcttta ttgacaacaa
     3661 acataataga aacttcacta gaaccttgag acatcatctg gatgttgatt ttgttttcag
     3721 atagagcgcg tgtcgcagta gcagtcactc cgatatggct cttcattttt tcaccaacaa
     3781 tcataatgat agaaaggtcg tgttcgattt ctgcatgatc tactttagcc ttttgaacca
     3841 actgacgcag gatttcttct tccttgatgg gagttagttg gcgagaacgg agaatgatag
     3901 aaagatcgtc gatacctgtt ggcatatgtt cccaaccgat gttaagttct tccaggattt
     3961 gcagaacctt gcggccaaat ccaacctcac ggttcatgag gtatttcgac atgttaatgc
     4021 tgacaaagcc tgagtcacca gcaattccca caactggaaa ttcatcatta ctgtgtttta
     4081 gaacgatacg agtacctgga tggtcagggt tgttggtatt cttgataacc agaggaattt
     4141 ttccacggta ggcaggaaga agagcctcgt catgaaggac tgagaagcct gcataggcca
     4201 actcgcgcat ttcacggtag gtcaactcag gaatcgagtg tggttggtgg ataataccag
     4261 ggtgggctgc aaagatacca tcaacgtccg taaagttttc atagaggtca gctttgacac
     4321 cagcagcaat gatagaacct gtaatatcag aacctccacg tgagaaagta cagatttgat
     4381 tttccttagt gacaccaaag aaaccaggaa tgacaaggac ttcattggtg tttgtcaatt
     4441 cttcaatctt gtcataactt gatggaatga tgcgagcgtg accaggttca cttgtgacca
     4501 caatcccagc ttctctaggg tgcatatagc gtgcatcgat accattttgg ttaaagtagg
     4561 cagcaatcaa tttggcattg ttattttcac cggctgctag gaaagtatcg tagagaaatt
     4621 cattttcttc aataggaaga gtggccaagg cgtgaatgct tttagaaatt ttttctagca
     4681 cagctggttt tagtcccaat tcactaacca tagcagcata gcggtcgata atccagcttt
     4741 ggttcttgct aatatcgtta cccgcaacat agtcgcggta gtatttaatc agggcatccg
     4801 taaccttagt atcttcagca ttgcgtttac caggcgcaga aacgactaca aaacgacgct
     4861 ctgaatcgct tttgacgatg tttaaaactt tttctaattg actagcagag gcaagagaac
     4921 tacctccaaa tttaacaact ttcataagaa ctcctaaagt aagtatttta tacgattata
     4981 gcagaaagaa aggctttttt caatgaagaa aataagatgc tttctagcct aaagcgagcc
     5041 tttcttatcg gctgagacac tttcagtcga ttttttcttg ctttctgctc ctaaaaaaga
     5101 tatactttga cagtgaaata atttaacact caatgaaaat caaagagcaa actaagaagc
     5161 tagacgaagt cagctcaaaa tactgttttg aggttgcaga tggaagctga cgcggtttaa
     5221 agagattttc gaagagtata aactgcttat aaaataaaaa ggagccctga tggaacacat
     5281 tatttatcaa cttgaagagg acttggcaat ccttaccttg aaccgtcctg aggtcgcaaa
     5341 tggttttcat attcccatgt gtgaggagat tttagaagct ctgactttgg cagaagaaaa
     5401 tccagctgtg cattttatct taatcaatgc caatggaaag gtcttctcag ttgggggaga
     5461 tttggtagag atgaagcggg cagtggatga ggatgatatt ccatcattga caaaaatcgc
     5521 agagttggtc aatacgattt cttataaaat caagcaaata gccaaacctg ttttaatgga
     5581 agttgacggt gctgttgcag gtgccgcagc gaatatggct gttgcggcag atttctgttt
     5641 ggcgacggat aaggctaagt ttatccaagc ctttgttggt gttggtttgg ctccagatgc
     5701 aggggggatt catctcttga gccgcagtat cggtgtgacg cgtgctgctc aattagctat
     5761 gacaggagag gctttaacgg cagaaaaagc cctagaatgg gggttggttt accgcgtttc
     5821 tgaagctgaa aaacttgaaa agacgagaga acagcttctt aaaaaattaa gacgtgcttc
     5881 aagtaattcc tatgctgcca ttaagaagtt ggtttgggag agccaattta aagactggca
     5941 gggttacgct actttagaac tgaacctaca gaaatcctta gctcaaacag aggatttcaa
     6001 agaaggagtg cgagctcatt cggagagaag aagacctaaa tttacaggaa aataaaaaat
     6061 acttgcatca ttctttgaat tttgatataa ttctttctgt caaatgtttt gattgtaaaa
     6121 gttttttgaa gaaggaggga tatgaaaaat ggactaccaa cgaatcaatg aatatttaac
     6181 atctatattt aacaatgtcc ttgtaattga ggaagtgaac ttgagaggta gtcgttttaa
     6241 ggatatctcc atcaaagaaa tgcatacgat tgatgtcatc ggtaaggctc cagacgtgac
     6301 tccaagtcaa gtgtcaaaag agttgatggt aactcttgga actgttacga caagtttgaa
     6361 caatttagag cgtaagggct acattgagcg agttcggtca gaacaggatc gtcgtgtggt
     6421 gcatctgcat ttgacaaaga agggtcgctt gattcataga ctgcataaac gcttccacaa
     6481 ggccatggta gaaaaaatta ttgatggcat gagcgaggaa gaaattgctg tcatgggtaa
     6541 aggtttgact aatctttacc aatttttgga ggatttgaaa taatggcttt tgcaaaaata
     6601 agtcaggttg ctcattatgt gccagagcaa gtggttacaa atcacgactt ggctcagatt
     6661 atggatacca atgatgagtg gatttcaagt cgaacgggaa tacgacaaag gcatatttca
     6721 agaacagaat ctaccagtga tttggctaca gaggttgcta agaaactgat ggcaaaagct
     6781 ggaataacag gagaagaact ggattttatc atcctagcta ccattactcc agattcgatg
     6841 atgccctcta cagctgctcg tgttcaagct aatattggtg ctaataaagc ctttgctttt
     6901 gacttaaccg cggcttgcag tggatttgta tttgctcttt caactgctga aaagtttatc
     6961 gcttctggtc gctttcaaaa aggcttggtg attggtagtg aaaccctctc taaggcagtc
     7021 gattggtcgg atcgatcaac agctgtgttg tttggagatg gtgctggtgg tgtcttgtta
     7081 gaagctagcg agcaagagca tttcttagct gagagtctca atagcgatgg aagtcgcagc
     7141 gagtgtttaa cttatgggca ttcaggttta cattctccat tttcagatca agaaagtgca
     7201 gattcgtttt tgaagatgga tggacgcaca gtctttgatt ttgccattcg agatgtagcc
     7261 aagtctatca agcagactat tgatgaatct cctatagagg tgacagactt ggattatctg
     7321 ctacttcatc aagccaatga ccgtattttg gataagatgg ctagaaaaat tggtgttgac
     7381 cgagccaaac ttccagccaa tatgatggaa tatggcaata ccagtgcagc cagtatcccg
     7441 attttacttt cagagtgtgt agaacaaggt ctcatccctt tagatggtag ccagactgtt
     7501 cttctatcag gcttcggtgg aggcttgacc tggggcacgc tcattcttac aatttaggta
     7561 atcatgtggt gaacacattg ttataaaaaa actatttatt ttaaaggagt cctatcatgg
     7621 cagtatttga aaaagtacaa gaaattatcg ttgaagaact tggaaaagac gcatcagaag
     7681 taacacttga atcaactttt gatgatttgg acgcagattc attggacttg ttccaagtaa
     7741 tctcagaaat cgaagatgct tttgatatcc aaatcgaagc agaaaatgac ttgaaaacag
     7801 ttggtgactt ggttgcttac gttgaagagc aagcaaaata agcagaatat tagtagaagg
     7861 agtagggaga aacccactcc ctcttgttta ggtaatagtt tgactgtcaa attatggtga
     7921 aatcgaacta ttacgtaagc aaggaaggtt ggaggcacta tgaaaacgcg tattacagaa
     7981 ttattgaaga ttgattatcc tattttccaa ggagggatgg cctgggttgc tgatggtgat
     8041 ttggcagggg ctgtttccaa ggctggagga ttaggaatta tcggtggggg aaatgccccg
     8101 aaagaagttg tcaaggccaa tattgataaa atcaaatcat tgactgataa accctttggg
     8161 gtcaacatca tgctcttatc tccctttgtg gaagacatcg tggatctcgt tattgaagaa
     8221 ggtgttaaag ttgtcacaac aggagcagga aatccaagca agtatatgga acgtttccat
     8281 gaagctggga taatcgttat tcctgttgtt cctagtgtcg ctttagctaa acgcatggaa
     8341 aaaatcggtg cagacgctgt tattgcagaa ggaatggaag ctggggggca tatcggtaaa
     8401 ttaacaacca tgaccttggt gcgacaggta gccacagcta tatctattcc tgttattgct
     8461 gcaggaggaa ttgcggatgg tgaaggtgct gcggctggct ttatgctagg tgcagaggct
     8521 gtacaggtgg ggacacggtt tgtagttgca aaagagtcga atgcccatcc aaactacaag
     8581 gagaaaattt taaaagcaag ggatattgat actacgattt cagctcagca ctttggtcat
     8641 gctgttcgtg ctattaaaaa tcagttgact agagattttg aactggctga aaaagatgcc
     8701 tttaagcaag aagatcctga tttagaaatc tttgaacaaa tgggagcagg tgctctagcc
     8761 aaagcagttg ttcacggtga tgtggatggt ggctctgtta tggcaggtca aatcgcaggg
     8821 cttgtttcta aagaagaaac agctgaagaa atcctaaaag atttgtatta cggagccgct
     8881 aagaaaattc aagaagaagc ctctcgctgg acaggagttg taagaaatga ctaaaacagc
     8941 ctttttattt gctggtcaag gtgcccagta tctagggatg ggacgggatt tctatgatca
     9001 gtatccgatt gttaaagaaa cgattgatcg agcgagccag gtgctcggtt atgatttgcg
     9061 ttatctcatc gatacggaag aagacaaact caatcagacc cgctatacgc aaccagccat
     9121 tctagcgact tcggttgcta tctaccgttt attgcaagaa aagggctatc agcctgatat
     9181 ggttgctggt ttgtctcttg gagaatactc tgccttggtg gcaagtggcg ccttggattt
     9241 tgaagatgcg gttgccttgg tagctaagcg tggagcctat atggaagaag cggctcctgc
     9301 tgactctggc aagatggtag cagttctcaa tacgccagta gaggtcattg aagaagcctg
     9361 tcaaaaagct tctgaacttg gagtggttac tccagccaac tataacacac ctgcacaaat
     9421 cgtcattgct ggagaagtgg ttgcagttga tcgagcggtt gaacttttgc aagaagcagg
     9481 tgccaaacgc ttgattcctc ttaaggtgtc aggtcccttt cacaccgctc tccttgaacc
     9541 tgctagccag aaactagctg aaactctagc tcaggtaagt ttttcagatt ttacttgtcc
     9601 cctagtcggc aatacagaag ctgctgtgat gcaaaaagag gacattgctc agctcttgac
     9661 gcgtcaggtc aaggaacccg ttcgtttcta tgaaagtatt ggggtcatgc aagaagcagg
     9721 cataagcaac tttatcgaga ttggaccggg aaaagtcttg tcaggttttg ttaaaaaaat
     9781 tgatcaaact gctcacttag ctcatgtgga agatcaagcg agtttagtag cacttttaga
     9841 aaaatagact aaaataagta gaagttttga aaggaaaaaa atgaaactag aacataaaaa
     9901 tatctttatt acaggttcga gtcgtggaat tggtcttgcc atcgcccaca agtttgctca
     9961 agcaggagcc aacattgtct taaacagtcg tggggcaatc tcagaagaat tgctcgctga
    10021 gttttcaaac tatggtatca aggtggttcc catttcagga gatgtatcag attttgcaga
    10081 cgctaagcgt atgattgatc aagctattgc agaactgggt tcagtagatg ttttggtcaa
    10141 caatgcaggg attacccaag atactcttat gctcaagatg acagaagcag attttgaaaa
    10201 agtgctcaag gtcaatctga ctggtgcctt taatatgaca caatcagtct tgaaaccgat
    10261 gatgaaagcc agagaaggtg ctatcattaa tatgtctagt gttgttggtt tgatggggaa
    10321 tattggtcaa gctaattatg ctgcttctaa ggctggcttg attggcttta ccaagtctgt
    10381 ggcacgcgag gtcgctagtc ggaatatacg agtcaatgtg attgctccag gaatgattga
    10441 gtctgatatg accgctatcc tgtcagataa gattaaggaa gctacactag ctcagattcc
    10501 gatgaaagaa tttgggcagg cagagcaggt tgcagatttg acagtatttt tagcaggcca
    10561 agattatcta actggacaag tggttgccat tgatggtggc ttaagtatgt agcgaaagct
    10621 agaggtgaaa agaatgaaac taaatcgagt agtggtaaca ggttatggag taacatctcc
    10681 aatcggaaat acaccagaag aattttggaa tagtttagca actgggaaaa tcggcattgg
    10741 tggcattaca aaatttgatc atagtgactt tgatgtgcat aatgcggcag aaatccaaga
    10801 ttttccgttc gataaatact ttgtaaaaaa agataccaac cgttttgata actattcttt
    10861 atatgccttg tatgcagccc aagaggctgt aaatcatgcc aatcttgatg tagaggctct
    10921 taatagggat cgttttggtg ttatcgttgc atctggtatt ggtggaatca aggaaattga
    10981 agatcaggta cttcgccttc atgaaaaagg acccaaacgt gtcaaaccaa tgactcttcc
    11041 aaaagcttta ccaaatatgg cttctgggaa tgtagccatg cgttttggtg caaacggtgt
    11101 ttgtaaatct atcaatactg cctgctcttc atcaaatgat gcgattgggg atgccttccg
    11161 ctccattaag tttggtttcc aagatgtgat gttggtggga ggaacagaag cttctatcac
    11221 accttttgcc atcgctggtt tccaagcctt aacagctctc tctactacag aggatccaac
    11281 tcgtgcttcg atcccatttg ataaggatcg caatgggttt gttatgggtg aaggttcagg
    11341 gatgttggtt ctagaaagtc ttgaacacgc tgaaaaacgt ggagctacta tcctggctga
    11401 agtggttggt tacggaaata cttgtgatgc ctaccacatg acttctccac atccagaagg
    11461 tcagggagct atcaaggcca tcaaactagc cttggaagaa gctgagattt ctccagagca
    11521 agtagcctat gtcaatgctc acggaacgtc aactcctgcc aatgaaaaag gagaaagtgg
    11581 tgctatcgta gctgttcttg gtaaggaagt acctgtatca tcaaccaagt cttttacagg
    11641 acatttgctg ggggctgcgg gtgcagtaga agctatcgtc accatcgaag ctatgcgtca
    11701 taactttgta ccaatgacag ctgggacaag tgaagtatca gattatatcg aagctaatgt
    11761 cgtttatgga caaggcttgg agaaagaaat tccatacgct atttcaaata cttttggttt
    11821 tggaggccac aatgcagttc ttgctttcaa acgttgggag aatagataag tatgaattta
    11881 aacgatatta aagacttgat gactcaattt gaccagtcaa gtttgagaga attttcttat
    11941 aaaaatggga cggatgagtt gcagtttagc aagaatgaag caagacctgt gcctgaagtt
    12001 gcaactcaag tcgctccagc acccgttcta gcaacaccga gtccagtagc tcctacatct
    12061 gctccagcag agactgtagc agaagaagtt ccagctccag ctgaagcaag tgtggctact
    12121 gagggaaatc ttgtagagag tccacttgtt ggagtggttt acttggctgc tggaccagat
    12181 aaacctgcct tcgttacagt tggtgatagt gtcaaaaaag gtcaaacatt ggtaattatc
    12241 gaagccatga aagtcatgaa tgaaatccca gctcctaagg atggtgtggt aacggaaatt
    12301 ctcgtctcta acgaagaaat ggttgagttt ggtaaaggat tggtacgtat caaatgatcg
    12361 atattcaagg aatcaaagaa gctcttcccc accgttatcc tatgcttcta gtggaccgtg
    12421 tcttggaagt gagcgaggat accattgttg ctatcaaaaa tgtgaccatc aacgagcctt
    12481 tctttaacgg ccactttcct caatacccag ttatgccagg tgttgtgatt atggaagcct
    12541 tggcgcaaac tgccggtgtg ttggagttat caaaacctga aaataaagga aaactggtct
    12601 tttacgctgg tatggacaag gttaagttca agaagcaagt tgtaccaggc gaccaattgg
    12661 ttatgacagc gacttttgta aaacgtcgtg gcaccatagc tgtggttgaa gcaaaggctg
    12721 aagtggatgg caagcttgca gccagtggta cccttacttt tgcaattggg aactaaggag
    12781 gttctccatg tttcgaaaaa ttttaattgc caatcgtggt gaaattgcgg ttcgtattat
    12841 ccgtgcggca cgtgaattgg ggattgcgac ggtagcggtt tattcaactg ctgataagga
    12901 agctcttcat acgcttttgg cagatgaagc agtttgtatt ggtcctggca aggcaacaga
    12961 gtcttatctc aatattaatg cagttctatc agctgcagtc ttgactgagg cagaagctat
    13021 tcaccctggt tttggatttc tcagtgaaaa ttccaaattt gcgaccatgt gtgaagaagt
    13081 aggtatcaag tttatcggtc catctggtca tgttatggat atgatggggg ataagatcaa
    13141 tgcgcgtgct cagatgatta aagcaggtgt gcctgttata ccaggttcag atggagaagt
    13201 gcataactct gaagaagctt tgattgttgc tgaaaaaatt ggctatcctg ttatgctcaa
    13261 ggcttcagca ggtggaggtg gtaaagggat tcgtaaggtt gaaaaaccag atgacctcgt
    13321 ttctgccttt gaaactgcct ctagtgaggc caaggccaat tatggcaatg gtgccatgta
    13381 catagaacgg gttatctatc cagctcggca cattgaggtt caaatcctag gtgatgagca
    13441 tggacatgtg attcacttgg gtgaacggga ttgttctctt caaaggaata accaaaaggt
    13501 tttggaagaa agtccctcga ttgcaatcgg aaaaacgctg cgtcatgaaa taggtgctgc
    13561 tgctgttcga gcggcagagt ttgttggcta tgagaatgca ggaaccattg aatttcttct
    13621 tgatgaagca agtagcaatt tctatttcat ggagatgaat actcgtgttc aggtagaaca
    13681 tccagtaaca gagtttgttt caggtgttga tatcgttaag gaacagattt gcattgcggc
    13741 aggtcagcct ttgtctgtta agcaagaaga tattgtccta cgcggtcatg ccatcgagtg
    13801 tcgtatcaat gcagaaaacc cagcctttaa ctttgctcca agtccaggta agattactaa
    13861 tctctatctg ccaagtggtg gagttggctt gcgcgtggat tcagcagttt atccaggtta
    13921 taccattccg ccttattatg atagtatgat tgccaaaatc atagtacacg gcgaaaatcg
    13981 ttttgacgcc ttgatgaaaa tgcaacgtgc cctctatgaa ttagaaattg aaggagtgca
    14041 gaccaatgca gatttccagc ttgacctcat ttcagatcgc aatgtcattg ctggggatta
    14101 tgatacttcc ttcttgatgg aaaccttctt acctaaatat caagaaaaag aataaaatga
    14161 ctttaagagt ttaaaccgaa aaggggaatc aatggctcta tttagtaaaa aagataagta
    14221 tattcgaatc aatcccaatc gttcggttag ggaaaaacct caagctaagc cagaggttcc
    14281 agatgaatta ttttcccagt gtccaggctg taagcatacc atctatcaga aggatctggg
    14341 aagtgaacgt atctgtccgc actgtagcta tacctttcgt atttctgccc aagaacgctt
    14401 ggctttgacg attgatatgg gaaccttcaa agaattgttt acagggattg aaagcaagga
    14461 tcccttgcat ttccctggtt accaaaagaa actggcatct atgcgtgaaa aaacaggtct
    14521 gcatgaagcc gttgtgacag gaactgctct tattaaaggt cagactgtgg ctcttgggat
    14581 tatggattct aactttatca tggcttctat gggtacggtt gtaggtgaaa aaatcactcg
    14641 tttgtttgag tatgcgactg tcgaaaaatt gccagttgtc ctattcacag cctctggtgg
    14701 agcccgtatg caggaaggaa tcatgagtct catgcagatg gctaagatct ctgcggcggt
    14761 taaacgccat tcaaatgctg gtctctttta cctgaccatt ttgacagatc caacgactgg
    14821 tggtgtgaca gcttctttcg ctatggaagg cgatatcatt ctggctgaac cacagagctt
    14881 ggttggtttt gctgggcgtc gtgtgattga aaatacggtt cgtgaaagct tgcctgagga
    14941 tttccaaaag gcagaattcc tattagaaca tggctttgtg gatgctattg tcaaaagaag
    15001 agacttacca gatacgattg ctagcctagt cagattgcat ggagggagtc ctagatgaat
    15061 attgcaaaaa tagtcagaga agcgcgtgag cagagtcgct tgacaacctt ggactttgcg
    15121 acaggcattt ttgatgaatt tatccaatta catggtgacc gttcttttcg tgatgatggt
    15181 gcagttgttg gtggtattgg ttggcttgga gaccaagctg taacagtggt tggtatccaa
    15241 aaaggcaaga gtttgcaaga caacctcaaa cggaattttg gccaaccaca tccagaaggc
    15301 taccgaaagg cactgcggtt gatgaaacag gctgagaaat ttggccgtcc agttgtgacc
    15361 tttatcaata cagcaggtgc ttatcctggt gtcggagcgg aagaacgtgg tcaaggggaa
    15421 gctatcgctc gcaatctcat ggaaatgagt gacctgaaag ttcctattat cgccattatt
    15481 atcggtgaag gtggttcagg cggggctctg gctctagctg tcgcggaccg tgtctggatg
    15541 ctggaaaatt ctatctatgc cattctcagt ccagaaggct ttgcttccat tttatggaag
    15601 gacggtactc gcgctatgga agcagcagaa ctgatgaaaa tcacttcgca tgaactgtta
    15661 gaaatggacg tggtggataa ggtgatttct gaaataggac tttctagtaa agaactgatt
    15721 aagagtgtca aaaaagaact ccaaacggag ctggctagac tttcacaaaa accgctagaa
    15781 gagttgctgg aagaacgcta tcaacgattt agaaaatact aagaatgact aaatgagact
    15841 agaaaggtct cattttttat ggaaaaatgt aattcaggtc gaaaaattgc cagttgagga
    15901 gatgggctat aaaatagaca aaaaaagaaa tatactataa atgtgagcct acaacaatct
    15961 attttaagaa ggtacttgca atgacaggta caaatacatt tacagttctt tcaactgagg
    16021 acttggagca aacttcaggt ggtcttgctg tttgggaaga tggatatagt agatggttat
    16081 attatagaga atttgctccc tatatgaggc aaggggcact taattcttat atagatgctt
    16141 ggaagtacgg cttccgagca gggtaatctt tcaaaaaagt acgatgagaa acacaaaaaa
    16201 actaagacaa tttggaatat ttttgcttat tattttacta tctacctatt taccacaaac
    16261 aattggattg tatgtgacga tcattttagg gttaggagct gatgtctatt ctcttatttt
    16321 aacaatggga ttagtgggaa gtttccttct attaatttgg aggttaaaaa agaaaaagat
    16381 gctctttatc tttgagaaaa agagctggaa ctggtcattt gtcttctact tatttgcaac
    16441 ttatgtggtt tatcagattc tcggtaattt ttgggcacgc tatgctcatt tgattaatca
    16501 taggaatatt catgatgagt attttaccgt gcttttttca aatggacaac caaccttttt
    16561 atcgactatc ttatcttttg tcctgcctgt aatcatcggt cctgtttttg aggagactct
    16621 tgatagaggg tattttatga ataccttctt tccctagtcc aagtactatt tggatgttat
    16681 cctatctgct atcatctttg ggattagcca tttgatacta tctcacagag atccaa
//
LOCUS       DAVHEW010000041        16370 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVHEW010000041 DAVHEW010000000
VERSION     DAVHEW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.041"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..213
                     /locus_tag="VVL53_001206"
                     /pseudo
     CDS             1..213
                     /locus_tag="VVL53_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828316.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            564..1777
                     /locus_tag="VVL53_001207"
                     /pseudo
     CDS             564..1777
                     /locus_tag="VVL53_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
     gene            1764..3080
                     /locus_tag="VVL53_001208"
     CDS             1764..3080
                     /locus_tag="VVL53_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU4183385.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            3255..4241
                     /locus_tag="VVL53_001209"
     CDS             3255..4241
                     /locus_tag="VVL53_001209"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU4183386.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4305..4376
                     /locus_tag="VVL53_001210"
     tRNA            4305..4376
                     /locus_tag="VVL53_001210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4338..4340,aa:Arg,seq:ccg)
     gene            4559..5257
                     /locus_tag="VVL53_001211"
     CDS             4559..5257
                     /locus_tag="VVL53_001211"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU4183387.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTYLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5248..8787
                     /gene="smc"
                     /locus_tag="VVL53_001212"
     CDS             5248..8787
                     /gene="smc"
                     /locus_tag="VVL53_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU4183388.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELELTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKENCQERLAAISSDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKIDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEAIEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLDSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8784..9578
                     /locus_tag="VVL53_001213"
     CDS             8784..9578
                     /locus_tag="VVL53_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4183389.1"
                     /translation="MTIKLVATDMDGTFLDENGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            9578..10396
                     /locus_tag="VVL53_001214"
     CDS             9578..10396
                     /locus_tag="VVL53_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4183390.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYADVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVMPMTNDEEAVAWAIEEYVLKEN"
     gene            10400..11689
                     /gene="ftsY"
                     /locus_tag="VVL53_001215"
     CDS             10400..11689
                     /gene="ftsY"
                     /locus_tag="VVL53_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU4183391.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11733..13220)
                     /locus_tag="VVL53_001216"
     CDS             complement(11733..13220)
                     /locus_tag="VVL53_001216"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU4183392.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPID
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(13369..14109)
                     /locus_tag="VVL53_001217"
     CDS             complement(13369..14109)
                     /locus_tag="VVL53_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4183393.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14109..16274)
                     /locus_tag="VVL53_001218"
     CDS             complement(14109..16274)
                     /locus_tag="VVL53_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU4183394.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5094 a         2934 c         3627 g         4715 t
ORIGIN      
        1 caatgtcaat cgatgaatat gtaataggaa agggacagca aaataagtct ttatgctacg
       61 ctcttgagaa gggaaaatac aaaaatctat ttcttggaat ttctggtggc tcagcttcaa
      121 aatttggtat ttattggaat aaaaaaacaa acaaatataa agatcaagct aataatgaga
      181 tttcagagtt ggatcagcga ttttcaaaat taaaatcaga tttgtatgaa attatcaaag
      241 aaggtattcg ttttaacttt gaaaatccta tttttgatat gaaaagatca acaaatgaat
      301 ttattggtcg ttctgctatg gtgacaaaat tactttgtat ctattctgag ggagatcctt
      361 tctttggtgt aaatattaat agtcagaaag aattttggaa ccactttgtt tctcagacaa
      421 atcaaggtgg accttatctg caaaatcata aaataattga actggtgtcc aaaacttatc
      481 ctgagttgga gccatcgaaa ttaggaacta tgctttttga gtattctaag ctttttatgg
      541 aaaataagga agacaatagt acaatggatt catcaaacaa ttttcgtcat caattaactc
      601 aatctctatt aaagtctcca aaccttatcc ttcgtggtgc tcctggcaca ggaaagactt
      661 atcttgctaa agaaattgcc aaagaattaa cggatggcaa cgaagaccaa atcggatttg
      721 tacaatttca cccatcatat gattatacgg attttgtaga aggtttaaga ccagtatcaa
      781 atggggatgg agctattgag tttaggctac aggacggtat ttttaaagat ttttgtcaga
      841 aagcaaaaga aacccaattg attggaggac aagataattt tgatgaggct tgggattctt
      901 acttagaata tataaatgtt gctgaagaaa aagaatatat aacaaaaaca tcttacttat
      961 ctattaatag tagacaaata atagtagaca aaatttgtca gtaaattatg atagtggtgt
     1021 ttcaggatgg tcactaccta gcaaatatgt ttacgagttg tataaagata aaaattataa
     1081 taagcaagaa tactacaaaa gtggtggaaa aactgtccta gaaacattga gaaagagatt
     1141 tggtttgaaa gactatgttt ccccaacaga aattgatact gataagaaat tcgtcttcat
     1201 catcgatgaa atcaatcgtg gggagatttc taagattttt ggcgaactct ttttctctat
     1261 cgaccctggc tatcgtggtg aaaaaggaag tgtttctacc caatatgcaa atctacacga
     1321 aactgatgaa aagttctata tccccgaaaa tgtttacatc atcggaacta tgaatgatat
     1381 tgatcgttca gtggatacct ttgattttgc tatgcgtcgt cgttttcgtt ttgttgaagt
     1441 tactgccgag ggtcaagttg gcatgttgga taaagagctg aatatccatg cagaagaagc
     1501 aaaaattcgt ctaagaaact tgaacgctgc tatcgaaaat gttcaggaat taaacagtca
     1561 ttatcatatt ggaccaagtt attttcttaa gttgaaggat gtagattttg actatgaatt
     1621 actctggtct gattatatta agcctctcct agaagactac ttgcgaggtt cttatgatga
     1681 ggttgaaact ttggaaactt tgaaaaaagc atttgaactg acaaataatg atcaaactgg
     1741 tcaacaagat actggtgata atgatgcgga ttactgataa tcaatacaag attattaaag
     1801 aaaaatttgt tgaagaatat cctaaactaa gcaatcttct tttagacaga accttggaaa
     1861 gtctatccca agatgaacgt attttcattt ttccaaatga tttgactcat actcctgatt
     1921 tggataagga ccaaaagatt tttgaaacag tcaatcagaa aatcaagaca gggaacgtga
     1981 ttggttttct tggatatggt caggaaagat taacgatttc ctcacgattt tctgatgaga
     2041 gtaatgacca ttttttgcat tatctcttaa acaaggttct tcatatcaat ctcactagtt
     2101 tagatgttgc tttgtctcgt gaagagaggc tttatcaact tttgatgtat ctcttcccca
     2161 agtatctaca agctgctatt cgaaaaggtc tttataagga ataccagaga ttttctcata
     2221 acgacagtca tgttaaggga gtgattgatg taagaaacca tctcaagaaa aatcttcctt
     2281 tcacgggaaa tattgcctac acaacgagag agttcaccta tgataatccc ctcatgcagt
     2341 tggtccgtca cactattgaa tacattaaga atcagaaaag cattggtcaa ggggtactag
     2401 ataatctctc aactagtcgt gaaaacgtat ctgaaatcgt gcgtgtaacg ccctcttata
     2461 aactagctga tcgtgctaag attattcggg gaaatcaatc taaacctata cgtcatgcat
     2521 actttcacga gtacagaaac ttacaagaac tttgtctgat gatcctaaac caagaaaagc
     2581 acggtttagg gtatcaagat caaaaaatct atggtattct ctttgatgtt gcctggcttt
     2641 gggaagagta tgtttacacc ttgttgccaa aaggttttgt acatcccaga aataaggata
     2701 agacggatgg aatttcagta ttttctgttg ggaaacgaaa agtatatcca gatttttatg
     2761 acagagaacg aaagattgtt ctagatgcaa aatataaaaa actggaattg actgaaaaag
     2821 gaatcaaccg tgaggactta ttccagctga tttcctattc ttatatttta aaagctgaga
     2881 aggctggact tgtttttccg agcaaggaca aggttattga taatgagatg ggaaacctag
     2941 caggttatgg agctttgttg aagaaatggt ctatccaaat tcctcagaag tcttcatctt
     3001 atagtgcatt ttgtaaaatg atggaaaatt cagaagaaaa ttttaaagcg attattgatg
     3061 aagaagtggg gagaaaataa tttcctctct ttcttttatt tttaacacaa gtaaaacccg
     3121 aacaatatat ttcaattcat aaaataattt gacaaaaact gtactttggt ttataataaa
     3181 ttaaggaaac gtcggaaaag acgtagtgat gcgagagcat aggtaggtca ttacaaaaga
     3241 aacgagacat cgatatgtta aatgaatttc caatttttga ttacgaagat attcaattga
     3301 ttccaaataa atgtgtcatt aaaagccgtg cagaagcgga tacaagtgtc actttaggaa
     3361 atcacacctt taaactacct gttgtgccag cgaatatgca gacgattttg gatgaaaatg
     3421 tagcagagca actggctaaa ggtggttact tctacattat gcaccgtttt gatgaggcag
     3481 gacgcattcc ttttattaaa cgaatgcacg atcaagggct cattgcttct atctctgtcg
     3541 gtgttaagga ctatgagtat gatttcgtta gacagctcaa gactgatgct cctgagtata
     3601 tcactattga tattgctcat ggtcatgcgg atagcgtgat ttctatgatt caacacatca
     3661 agaaagaatt gccagatacc tttgtcattg ctggaaatgt gggaacacca gaagctgtgc
     3721 gtgaattgga aaatgctggt gcggatgcta ctaaggtcgg aatcggtcct ggtaaggttt
     3781 gtatcaccaa ggttaagact ggttttggta caggtggttg gcagttggct gctctacgct
     3841 ggtgtgccaa ggctgcgcgt aaaccgatta tcgctgatgg aggaattcgt actcacggtg
     3901 atattgccaa gtctatccgc ttcggtgcta gcatgatcat gattggttcc ctctttgcag
     3961 gacacattga aagtccaggg aaaacgattg aagtcgatgg tgaacagttc aaagaatatt
     4021 atggttcagc ctcacaatat cagaaaggtg cttacaaaaa tgtggaaggc aaacgtatct
     4081 tgcttcctgc taaaggtcat ctgcaagaca ctttaactga gatggaacaa gaccttcaaa
     4141 gtgctatttc ttatgcaggt ggacgtcagg ttgctgacct taaacacgtt gattatgtga
     4201 tcgtgaaaaa ctcaatctgg aatggggatg cttcccacta agacggggtc tttgactaaa
     4261 aaacttgtta ttagagcaaa tttctgttat aataaaacaa gtttccaccc ttagtgtaat
     4321 ggatatcacg taagattccg gttcttgaga tgggggttcg attccctcag ggtggatgta
     4381 aacatcctaa aaagccttta atagggcttt tttctttaca ctgctctaaa tttgccgcta
     4441 aaactgagaa tctctgtcta aagaattccc aaatgctttc cagtcccgtt ttagagtgac
     4501 tcagggggct ttttttgata taataaaaag gactgttatc agttagaaag aggttggtat
     4561 gaaagaatta caaactgtac taaagaacca ttttgcaatc gaatttacag acaaaaattt
     4621 actggaaact gcctttactc atacgagtta tgccaatgag caccgcctct taaaaatttc
     4681 acacaatgaa cgcttggaat ttttaggaga cgctgttcta cagttattga tttcagaata
     4741 tctgtataaa aaatatccta aaaagcctga aggtgaccta tcaaaactcc gtgctatgat
     4801 tgtccgtgag gagagtttag ctggttttgc gcgtgattgc cagtttgacc agtttatcaa
     4861 gttgggtaaa ggggaagaaa aatcaggtgg tcgcaatcgt gacaccattc ttggtgatgc
     4921 ctttgaagcc tttcttggtg cccttctttt ggataaggat gtggccaagg tcaaggaatt
     4981 tatctatcaa gtcatgattc ctaaggttga agcaggcgag tttgagatga ttacagacta
     5041 taaaacctat ctccaagagt tgcttcaggt caatggtgat gtggctattc gttatcaggt
     5101 gatttctgaa acagggcctg ctcacgataa ggtctttgat gtagaagttc ttgttgaagg
     5161 taagagcatc ggtcaaggcc aaggtcgttc taagaaatta gcagagcagg aagctgccaa
     5221 aaatgccgtt gagaaagggc tggattcatg tatttaaagg aaatcgaaat tcaggggttc
     5281 aagtcttttg ctgataagac caaggtcgtt tttgaccaag gtgtgacggc agttgttgga
     5341 cccaatggat ctggaaagtc caatattaca gaaagtctgc gttgggcttt gggggagtct
     5401 agtgtcaaga gtctccgtgg gggcaagatg ccggatgtca tctttgctgg aaccgaaagt
     5461 cgcaaaccgc tcaattatgc ttctgtagtt gtgactctgg ataatcatga cggatttatc
     5521 aaggatgcag gtcaagaaat cagggtggaa cgccatatct atcgtagtgg agatagcgaa
     5581 tacaagattg acggcaagaa agtccgtctg cgtgatattc atgacctctt cttggatact
     5641 ggattgggac gagattcctt ctctattatt tcccaaggga aggttgagga gatttttaat
     5701 tccaagtctg aggaacgacg agctattttt gaagaagctg ctggagtttt aaaatacaag
     5761 actcgcagaa aagaaaccga gagtaaactg cagcaaactc aggataatct ggaccgctta
     5821 gaggacatta tctacgagtt ggataatcaa atcaagcctc ttgagaagca agctgagaat
     5881 gcccgtaagt ttttagactt ggaaggacaa cgtaaggcta tttatttaga cgttctggtt
     5941 gctcaaatca aggaaaataa ggcagaacta gagttgacag aagaagagct ggctcaggtt
     6001 caagaactct tgatgagtta ttaccaaaag cgtgaaaaat tagaagaaga aaatcaaact
     6061 cttaaaaagc aacgccaaga tttacaggct gaaatggcca aagaccaagg cagtttgatg
     6121 gacttgacta gtctgattag tgatttagaa agaaaattag ccctatcgaa actggagtcc
     6181 gagcaagtgg ccttgaatca acaggaggca caagctcgtt tggctgcttt ggaggataag
     6241 agaaattcac tcagcaaaga aaagtatgat aaagaaagct ctttagctct gttagaggga
     6301 aatctagtcc aaaataatca aaaactcaat cgtttagaag ctgaattgct ggctttctca
     6361 gacgatcctg atcagatgat tgagctctta cgtgaacgct ttgtagctct tttacaagaa
     6421 gaagcggatg tctcaaacca gttgacccgt attgagaatg agttggaaaa tagtcgtcag
     6481 ctttctcaaa aacaagcaga tcaactagaa aagctgaaag agcaattggc tatagctaaa
     6541 gagaaggcta gtcagcaaaa ggaagagctt gaaactgcca aggagcaggt tcagaaatta
     6601 ttggctgact accaagctat tgccaaggag caagaggagc agaaaacttc ctatcaagct
     6661 caacaaagcc aactctttga ccgtctggat aatctcaaaa acaagcaggc cagagctcaa
     6721 agtttggaaa atatcctgag aaatcatagt aacttttatg caggtgttaa gagtgttctc
     6781 caagaaaaag atcgcctagg tgggattatt ggtgcagtca gtgagcatct gacctttgat
     6841 gtttattatc aaactgccct agagattgcc ttaggggcaa gtagccagca tatcatcgta
     6901 gaagatgaag agtcggcaac caaggctatt gacttcctta aacggaacag agccggtcgt
     6961 gcaacctttc ttcctttgac cactatcaag gcgcgtacga tttctagtca gaaccaagat
     7021 gctatcgctg taagcccagg tttccttggg atggcagatg agttggtgac ttttgatact
     7081 agactggaag ccattttcaa gaacttgcta gctacgacgg ctatctttga taccgtagaa
     7141 catgcgcgtg cagcagctcg ccaagttcgt tatcaggttc gtatggtgac attggatggg
     7201 acagagttgc gcacaggtgg ttcttatgca ggtggtgcca atcgccagaa taacagcatt
     7261 tttatcaagc cagaactgga gcaattacaa aaagaaattg ctgaagaaga agcaagcttg
     7321 ggttcagaag aggcgacttt gaagaccttg caagatgaga tggccagatt gaccgagtca
     7381 ttagaagcta ttaaatctca aggagagcag gcacgtattc aggagcaagg cttgttcctc
     7441 gcttatcagc aaactagtca gcaagttgaa gaactggaaa ctctttggaa actccaagaa
     7501 gaggaaatag atcgtctttc cgagggagat tggcaagcgg ataaggaaaa ctgccaagag
     7561 cgtcttgctg caatctccag tgacaagcaa aatctggaag ctgagattga agagattaag
     7621 tctaacaaaa atgccatcca agaacgctat caaaacttgc aggaagagct agcgcaagct
     7681 cgtttgctta agacagaact gcaagggcaa aaacgttatg aagttgctga tattgaacgc
     7741 ttaggcaagg aattggacaa tcttgatttt gaacaagagg aaatccagcg ccttcttcaa
     7801 gaaaagattg ataatcttga gaaggttgat acagaattgc tcagtcaaca ggcggaagaa
     7861 tccaaaactc agaaaacgaa cctccaacaa ggtttgattc gcaaacagtt tgagttggat
     7921 gatatagaag gtcagctgga tgatattgct agtcatttgg atcaggctcg ccagcagaat
     7981 gaggagtgga ttcgcaagca aacacgtgct gaagctaaga aagaaaaggt cagcgagcgc
     8041 ttgcgccatc tacaaagtca attaacagac cagtaccaga ttagctatac tgaagctata
     8101 gaaaaagcgc atgaattgga aaacctcaat ctggcagagc aagaagttaa ggatttagag
     8161 aaggctattc gctcactggg tcctgtcaac ttggaagcta ttgaccagta cgaagaagtt
     8221 cacaatcgtc tggatttctt ggatagccaa cgtgatgaca ttttgtcagc gaaaaacttg
     8281 ctccttgaga ccattacaga gatgaatgac gaggtcaagg aacgctttaa atcaaccttt
     8341 gaagctattc gtgagtcctt taaagtgacc ttcaagcaga tgtttggcgg aggtcaggca
     8401 gacttgatat tgactgaggg cgacctttta acagctggtg tggagatttc tgttcaacct
     8461 ccaggtaaga aaatccagtc gcttaacctc atgagtggtg gtgaaaaagc tctatcggct
     8521 cttgccttgc tcttctccat tattcgtgtc aagaccattc catttgtgat tttggatgag
     8581 gtagaggctg cgctagacga agccaatgtc aaacgctttg gagattatct caaccgcttt
     8641 gacaaggaca gccagtttat cgtcgtaacc caccgtaagg gaaccatggc agcggccgat
     8701 tccatctatg gagtgaccat gcaagaatcg ggtgtttcaa agattgtttc agttaagtta
     8761 aaagatttag aaagtattga aggatgacaa ttaaactagt agcaacggat atggacggaa
     8821 ccttcctaga tgagaatggg cgctttgata tggaccgcct caagtctctc ttggtttcct
     8881 acaaggaaaa agggatttac tttgcggtgg cttcgggtcg gggatttctg tctctggaaa
     8941 aattatttgc tggtgttcgt gatgacatta ttttcatcgc ggaaaatggc agtttggtag
     9001 agtatcaagg tcaggacttg tatgaagcga ctatgtctcg tgacttttat ctggcaactt
     9061 ttgaaaagct gaaaacgtca ccttatatag atatcaataa actgctcttg acgggtaaga
     9121 agggttcata tgttctagat acggttgatg agacctattt gaaagtgagt cagcattata
     9181 atgaaaatat ccaaaaagta gcgagtttgg aagatatcac agatgacatt ttcaaattta
     9241 caaccaactt cacagaagaa acgctagaag ctggtgaagc ttgggtcaat gataatgtcc
     9301 ctggtgtcaa ggctatgaca actggctttg aatctattga tattgttctg gactatgtcg
     9361 ataagggtgt agctattgtt gaattagcta aaaaacttgg catcacaatg gatcaggtca
     9421 tggcttttgg agacaatctg aatgacttac atatgatgca ggttgtggga catcctgtag
     9481 ctcctgaaaa tgcacgacca gagattttag aattagcaaa gactgtgatt ggtcaccata
     9541 aggaccagtc ggttatagct tatatggagg gcttataatg gcagatataa aattaatcgc
     9601 attggacttg gacggtacct tgctgactac tgataaaagg ctgacggatc gcaccaaggc
     9661 aactttgaaa gctgcgcgtg atcgtggtat caaggtcgta ttgacaactg gtcgtccctt
     9721 aaaagccatg gacttctttc tccatgagtt agggactgac ggtcaggaag atgagtatac
     9781 cattactttt aatggtggtt tggttcagaa aaatacaggt gaaatccttg ataaaacagt
     9841 cttttcatat gctgatgtgg cacgcttgta tgagaaaaca gagaaattat cactgccact
     9901 tgatgccatc tcagaaggaa cagtttatca aatccaatcg gaccaagaaa gtctttatgc
     9961 caaattcaat ccagctttga cttttgttcc agttgacttt gaagacctgt ctagtcaaat
    10021 gacctacaac aaatgcgtga ctgcctttgc tcaagaaccc ttggatgcag ccattcagaa
    10081 gatttctcca gaattgtttg accaatatga aatctttaaa tcacgtgaaa tgttgctaga
    10141 gtggtcacca aagaatgttc ataaagcaac aggtttggca aaactaatca gccatcttgg
    10201 aatcaaccaa agccaagtta tggcctgtgg tgacgaggcc aatgaccttt ctatgattga
    10261 atgggcaggt cttggtgttg ctatgcaaaa cgctgttcct gaagtaaagg cagccgcaaa
    10321 tgtagtaatg ccgatgacca acgatgagga agctgtcgcc tgggctatcg aagaatatgt
    10381 gctaaaggag aactaagata tgggattgtt tgaccgtcta ttcggaaaaa aagaagaacc
    10441 taaaatcgaa gaagttgtaa aagaagctct ggaaaatctt gatttgtctg aagatgttga
    10501 tcctaccttc acagaagttg aggaagttcc tcaggaagaa gcagaggttg aaattgttga
    10561 acaagctgtg ttccaagagg aggaaatcca agacacagtt gaagaaagtc aggattcaga
    10621 gccagctgta ggggtttctc aagaagaaat agaagaatta ccaaactcag aagaattacc
    10681 agaggaagag aagcttgagc acgaaggaac tgtagaagaa aataattctg aagttcttga
    10741 accagaaagg cctcaaacag aagaaactgt tcaggaaaaa tatgaccgca gtctcaagaa
    10801 gactcgtacg ggcttcggtg ctcgcttgaa cgccttcttt gctaacttcc gctctgttga
    10861 cgaagaattt ttcgaggaac tggaagaact gctgattatg agtgatgttg gtgtccaagt
    10921 cgcttctaac ttaacggagg aactacgtta cgaagccaag cttgaaaatg ccaagaaacc
    10981 tgatgcactt cgtcgtgtca tcattgagaa attggttgag ctttatgaaa aggatggtag
    11041 ctacgatgaa agcatccact tccaagataa cttgacagtt atgctctttg ttggtgtgaa
    11101 tggtgttggg aaaacaactt ctattggaaa actagcccac cgctacaaac gaactggtaa
    11161 gaaggtcatg ctggttgcag cagatacctt ccgtgcgggt gcagtagctc agctagctga
    11221 atggggccga cgagtagatg ttccagtagt aactggacct gaaaaagctg atccagccag
    11281 cgtggtcttt gatggtatgg aacgtgccgt ggctgaaggt atcgatattc tcatgattga
    11341 tactgctggt cgtctgcaaa ataaggataa ccttatggct gagttggaaa agattggtcg
    11401 tattatcaaa cgtgttgtgc cagaagcacc acatgaaacc ttcttggcac ttgatgcatc
    11461 aacaggtcaa aatgccctag tacaggccaa agaattttcg aaaatcacac ctttaacggg
    11521 aattgttttg actaagattg atggaactgc tcgaggaggt gtggttctag ccattcgtga
    11581 agaactcaat attcctgtaa aattgattgg ttttggtgaa aaaatcgatg atattggaga
    11641 gtttaactca gaaaacttta tgaaaggtct cttggaaggc ttaatctaat cagaagcaaa
    11701 aatcctgcaa ggtacaaact tgcaggattt ttttattcga aacgaccatc ttgacgatag
    11761 gcgatatctg gttgccaagt ccatttggca tcgaattttt caagtaggtc aaagctggct
    11821 tgaggtccca tgcttccagc tttatagtca tgaagtgggg caccattttc ggcccagagc
    11881 tcttcaatac ggtcaatcaa cttccatgac gcaccaacct catcccagtg gctaaagtta
    11941 gttgagttgt tatttaggac atcataaatc aatttctcgt atggttctgg agaagcacca
    12001 gttgcagtcg cgtctgtacg ataatcaagt gagttaggag ccaagttaaa ttcttctcct
    12061 acttgcttcc catttaggct aagagagaag ccttctgttg gttgaatata gatggtcaaa
    12121 atatttggag caagtggttc tccaaagata gaatccattt gtttaaagac gatgttgaca
    12181 tgagttcctt tttcagtcag tcgtttacct gtacggaaaa agaaaggaac accacggaat
    12241 cgatcgctgt ctacaaagaa ggcaccagat gtaaaggttt cagttgttga ttctggattc
    12301 acatttggtt cgctacgata agagatgtat ttcatgccat caatcttacc agagcggtat
    12361 tgtccacgga taaagtgttc tttgagttct tcatcaattg gatgatagag gtttttaaag
    12421 accttaatct tttcagcacg aatctcgtct tttgtgaagc ttgctggttt gtccatggcg
    12481 aggagcgaaa gaagttgtag agtgtggttt tggaccatgt cacggagggc accggattgg
    12541 tcatagtagc caccacgttc ttctacaccc aagcgctccg caaaggtaat ttgaacattg
    12601 tcgataaaat ccttgttcca aacgttttca aaaatcaagt ttgcaaagcg aactgcaaag
    12661 atgctttgga tcatttcctt gccaagataa tggtcgatac ggaaaatttg ttcttcgtca
    12721 aatgttgcta ggagttcgtc attcaacttg cttgcagttg cgtaatctgt accaaatggt
    12781 ttttcaacga tcaagcgctc aaaacctttg ccatcgacaa tgttttcaga tttgaggtgt
    12841 ttggcaatgg ttccaaagaa ctgaggtgcc atagacaaga agaagagctt attgtgttca
    12901 gcttggtatt tttcattaag ctcagcttgt aattgacgca aagcaatata atgttccgaa
    12961 tcattgacat catggctttg atagtagaag tggctagcaa attcttgggc ttgctcggta
    13021 ctatctgcca aatcaaggat ggactcgaca actacagatt caaaatattc cttactccaa
    13081 ggtctacggg cagttccaat aacggcaaag tgcttggaaa gattgccgga tttatatagt
    13141 ctaaaaaggg aagggtagag cttgcgttta gccaggtctc cactcgcacc gaaaattgta
    13201 acaataacct tagatgacat ctagctacct aatttctatt tttattccta gtcttgctag
    13261 gtatgaggaa acctcatttc ataaacttaa ttctaacata attttccgaa aaatcaaaaa
    13321 ttccaatacg aggtagaaaa actgcaagga aatccttaca gtttgagttt agacgtttaa
    13381 gaccttatct aagaactctt tcagacgagg gtgttgtggg ttatcaaaga tttggtcagg
    13441 tgttccgtct tcaaggaact cgccatctgc agtaaagata acgcggttgg caacctgacg
    13501 agcaaatccc atctcatggg ttacgataat catggtcatg ccttgctcag ccaattcctt
    13561 cataacgtta agtacgtctc caaccatctc agggtcaagg gcagaagttg gttcatcgaa
    13621 gagcatgatg tctggattca ttgctaggcc acgagcgatg gccacacgtt gtttttgacc
    13681 acctgatagg ctatctggat tggcattagc tttatctgct agtccaacct tttcaagcaa
    13741 ctccattccc aattcctcag cttcttcctt agtcatcaac ttgtgctcaa taggagcaaa
    13801 ggtgatgttg tccaatacag acatatgagg gaagaggttg aagtgttgga ataccatgcc
    13861 gatattttca cggacgtggt caacattggt tgttttttca gttaaatcat agccgttcac
    13921 agtgatgtga ccgctagtga cttcttctaa aagattgagg ctacggagga aagttgactt
    13981 accagaacct gaaggaccga tgatacaaac aacatctcct tcatagaact tagtcgtaat
    14041 tccttttagg acttcatttt ttccatagtg cttgtgtaaa tcatttacat caatttttaa
    14101 ttttgccatt aacgaatcct cttttctaag cgtttcgcta gtctagtcaa aagtgtgata
    14161 attacaagat agaagatagc aaggattgca tacatcttga aactttggta gttacgagca
    14221 atgataatct taccagtttg gaagagttca accaaaccga tagcagatac gatagttgta
    14281 tctttaagag cgataacgaa ttggttgaca aagtttggca acatcaattt agttgcttgt
    14341 ggcaagataa tcttacgcat ggtttttcca taagagatac ccaagcttcg gctggcttcc
    14401 atttggccaa ctggaacggc ctgaatacca ccacgaacga tttcagcgat ataagcagcc
    14461 gcattgagtg agagggcaat tgttccagct acaaagtcgt taattgggct ttgttggcct
    14521 gtgatagact cgatgaagtt tggaattccc cagaagatga aggctgcaag aatcatcaat
    14581 ggaataccac gaataacgtc aacgaaaatc tcagagataa cgcgaagaga tttgtatggg
    14641 ctaacgctaa acataccgaa gataattccg atgacaatgg caatagcaaa tgagataaga
    14701 gctagagcaa gagtgatacc aagaccgcta aggagttgtt tgtagttgtt ttgaagcaag
    14761 ccccagagcg ttgtttcgtc aacagtactt gttgaagcag ttgaagattc gctagctagg
    14821 tatttgtcaa gaatcttttg gaattcaccg tttgctttaa ggtttgcaag tccgttgttg
    14881 aacatttcaa tcagttctgg atttgctcct tttttaacgg caaaggctgt ttcaccgatt
    14941 ggagttccag agattggagt tttcaatttt tgaccttggc tgatagaata tttgagaaca
    15001 ggttcatcat ccataacggc atcaatggca ccagtgttta aactgtcata cattgaagaa
    15061 ccatcagcaa aggttttgat tttgtagccg tatttgcttt gattttctgt taggaaggtt
    15121 tgagaagcag ttccgttttt aacaccgact gtctttcctt ttagatcttc ataagaagca
    15181 atattgcttg attctttgac accaagaatg gtattagcag tgtagtatga ttctgagaag
    15241 tcaaaagttg ccttacgagc atctgtgaca gacataccag cgatgatacc atcggcttga
    15301 ccagcttgga cagcactgat agcagcatca aaaccagggt tggtgatttc aatttcaaaa
    15361 ccttggtctt tagcgattgc cttaatcaat tccatatcaa taccagtgta ttggttgctt
    15421 gaattttgga aaacaaaagg tgcaaaagaa gaatcgctgg caatgatata tttagcctta
    15481 acaggaatag cttttaatcc tgcgagagta gttgtagttg gcactgctga agatgatgaa
    15541 gcagtccatt tcttgataat tttatcaaga ctaccatctt ttttcatttc agacaaggct
    15601 tggttaaatt cagtaaccag gtgctcgtat ttacttcctt ttttcacacc gaaagcaaaa
    15661 cttcctacag cttcaccatc catttcaata tggaggtctt gaccttggtt aatggcatat
    15721 tcgataacag gtttgtcatc catcatggca tcgatggcac cagcactcaa gctgttgttc
    15781 attaaatcac cagtgtcaaa tgttttaata gtaaagccgt atttatcttt gattgtttca
    15841 aggaaacgtt gagcggcagt tccgttttta acaccaacgg ttttgccagt taattggtcg
    15901 tacttgctaa ttttgtgtga ctttgtagta gcaatgacaa cttttgtatc atagtaagta
    15961 tcagacatgg tgaagacttt ttcacgttct ttagtctttg tcatccctgc catgatagcg
    16021 tcggcttgcc cagcttgaac cgcattgact gctgcgtcaa atccaggata ggacatctga
    16081 atgttccagc ctttaatctc agcgactttg ttaataatgt caacatcaat tcctttataa
    16141 gtttgatctg aatctttaaa ctcaaaaggt gcataggcgg tatcagaaac aatcttaatc
    16201 gtttctgctt tggcaatacc taatgagaaa attgggaata aaattagcaa aaatgctaga
    16261 aattttttct tcatttttag tctccttttc cgaatattct ctcattatat cagaaaaagt
    16321 aaaaaaaggc aaatttttat attttttgtg tcagaaaata taacatcgat
//
LOCUS       DAVHEW010000042        15709 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVHEW010000042 DAVHEW010000000
VERSION     DAVHEW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.042"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..252
                     /locus_tag="VVL53_001219"
     CDS             <1..252
                     /locus_tag="VVL53_001219"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183395.1"
                     /translation="YFKEVKDKATKGLTTFEVNGSSVSSYDDLLTLFKEAVKKDAETL
                     KQEANGNKTVSMNNTVKLKEAVYKKLLQQTNSFKTSIFK"
     gene            313..2034
                     /gene="pabB"
                     /locus_tag="VVL53_001220"
     CDS             313..2034
                     /gene="pabB"
                     /locus_tag="VVL53_001220"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU4183396.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDIVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMTVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            2035..2652
                     /locus_tag="VVL53_001221"
     CDS             2035..2652
                     /locus_tag="VVL53_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4183397.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTYPYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            2702..3700
                     /locus_tag="VVL53_001222"
     CDS             2702..3700
                     /locus_tag="VVL53_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU4183398.1"
                     /translation="MNKRLFSKMSLVTLPILALFSQSVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEVQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            3827..4786
                     /locus_tag="VVL53_001223"
     CDS             3827..4786
                     /locus_tag="VVL53_001223"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU4183399.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            4883..5722
                     /locus_tag="VVL53_001224"
     CDS             4883..5722
                     /locus_tag="VVL53_001224"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU4183400.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(5770..5940)
                     /locus_tag="VVL53_001225"
     CDS             complement(5770..5940)
                     /locus_tag="VVL53_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU4183401.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            6061..6945
                     /gene="miaA"
                     /locus_tag="VVL53_001226"
     CDS             6061..6945
                     /gene="miaA"
                     /locus_tag="VVL53_001226"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU4183402.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            6938..8176
                     /gene="hflX"
                     /locus_tag="VVL53_001227"
     CDS             6938..8176
                     /gene="hflX"
                     /locus_tag="VVL53_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU4183403.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            8169..8792
                     /locus_tag="VVL53_001228"
     CDS             8169..8792
                     /locus_tag="VVL53_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU4183404.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            8807..9736
                     /locus_tag="VVL53_001229"
     CDS             8807..9736
                     /locus_tag="VVL53_001229"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU4183405.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            9757..10512
                     /locus_tag="VVL53_001230"
     CDS             9757..10512
                     /locus_tag="VVL53_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4183406.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(10566..11474)
                     /locus_tag="VVL53_001231"
     CDS             complement(10566..11474)
                     /locus_tag="VVL53_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU4183407.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            11538..11807
                     /locus_tag="VVL53_001232"
     CDS             11538..11807
                     /locus_tag="VVL53_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU4183408.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            11807..12187
                     /locus_tag="VVL53_001233"
     CDS             11807..12187
                     /locus_tag="VVL53_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4183409.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            12276..12503
                     /locus_tag="VVL53_001234"
     CDS             12276..12503
                     /locus_tag="VVL53_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183410.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(12543..13268)
                     /locus_tag="VVL53_001235"
     CDS             complement(12543..13268)
                     /locus_tag="VVL53_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU4183411.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGAVTANKDKELPTVMDLLPSNIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDDTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            13430..15271
                     /gene="typA"
                     /locus_tag="VVL53_001236"
     CDS             13430..15271
                     /gene="typA"
                     /locus_tag="VVL53_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU4183412.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGIKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            15291..15545
                     /locus_tag="VVL53_001237"
     CDS             15291..15545
                     /locus_tag="VVL53_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU4183413.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         4651 a         2985 c         3563 g         4510 t
ORIGIN      
        1 tacttcaaag aagttaaaga taaagcaacg aaaggattaa caacattcga agtaaatggt
       61 tcttccgttt catcatacga tgatttactg acattgttta aagaagctgt taaaaaagat
      121 gccgaaactc ttaaacaaga agcaaacggt aataaaacag tatctatgaa taatacagtt
      181 aaattaaaag aagctgttta taagaaactt cttcaacaaa caaatagctt taaaacttca
      241 atctttaaat aattgaaatc tctcatctgc tttgcggatg agggttttct tattttatgc
      301 tatacttaag gtatgcatag aaaaacagtg attgatttta ggactttggg ggagagatat
      361 acctttaccc agcctattaa agagttgaaa acgagaaatg tagaagaagt ggcagatttg
      421 ctggcacaag tggaaagcta ccaagagcaa ggttattatg tggtggggta tgtcagctac
      481 gaggctgcac ctgcttttga ggaaaaatta gcagttcaca aggatcccct actgggagag
      541 tacctgcttt actttactgt tcatgatatc gtagaaactt cccctattcc tctgacttat
      601 gaggatattg atttgccctc aaattggcag gaagtaacgt ctgcagcaga ctatgaaaag
      661 gccattgccc aaattcacca tcatttacgg cagggagata cctatcaggt caactacacc
      721 gtccaactta agcaaaagtt aaatgccaat ccttttgcca tctacaatcg tatggtggta
      781 gagcaggagg cgggctacaa tgcctatgtg gaacatgacg agatgacagt gatttccatg
      841 agcccagagc tcttttttga gcaaaatgat cgcgagttga caacacgacc aatgaagggg
      901 acgactcagc gtggggtaac tgaccaagaa gatcttgaac aggccagttg gttggaacag
      961 gatcccaaaa atcgctctga aaatatgatg attgtggacc tcttgcgcaa tgatatgaac
     1021 cgtatttctg aagttgggag cgagcacgtg gagcgtctgt gtcaggtaga gcagtattca
     1081 actgtttggc agatgacttc gaccatcaag agtcagttgc gagaggatgt ggaccttgtt
     1141 gaaatcttcc gctcactctt tccttgtggt tccataacgg gtgcaccgaa aattgcgaca
     1201 atggagatta tcaaggactt ggagcctcaa ccgcgtggag tctactgtgg aacgattggt
     1261 ctcttgcttc caaatggacg acggattttt aatgtggcca ttcgtaccat tcaacttcac
     1321 aaaggtcaag ccatttatgg agttggcgga gggattactt gggatagcac atgggaatct
     1381 gaataccgag aggttcatca aaaggcagca gttctctatc gtaaacaagc tcgcttccaa
     1441 ttaattacaa ctggaaaaat cagccaaaaa caactgatgt ttgaagaaca acatctggaa
     1501 aggctgagaa aagctagtcg ttattttgct tttccttttg atgcagaaga cttgggacac
     1561 aagattgagg aagagtgtca ggattgtgaa gctaatcgag attaccgctt gcgaatcagc
     1621 cttagcaaat ctggagagat agaagtcaat cgtcaagtat taacccctct cagtacaagc
     1681 ttttgtcagg cccaagtctg tcttcaggaa gctgctttga atcaatcctt tacctacttt
     1741 aaaaccactc accgaccgca tttgagccta ggagaacaag agaagattta ccacaataag
     1801 tcaggagaac tgcttgaaac ctctatagga aatttggttc tgaaaatcgc tggaaaactc
     1861 tacacaccgc ctatccgact tggaatcttg ccaggaattt accgtcagca tttgctagaa
     1921 acaggacagg tagaagagaa agtcttgacc ttggcagatt taacccaagc agaagctatt
     1981 tacggctgta atgcagtgag aggcttgtat gaattaagcc ttgaggagaa ctagatgaaa
     2041 ttaatatttt tgcatgggtt agggcagtca gcagagtctt ggaaagaagt tcggaatcta
     2101 ctgacagatt atccttctga ggctatcgag ttatttcctt ctggagttag taactatcaa
     2161 caagctaaag agcgagttta tcagcaccta gctcaagaga cagaaccttt tgttttgatt
     2221 ggattgtcct taggtgctgc cctgacacta gaactttcca gttacgattt accaaatctt
     2281 cgagctttga ttctgtcagg atgcccgctt aaattagcag ggaatatcct attttatctt
     2341 caattgctga tttttaaact acttcccaaa agagtatttg aaaaacaggg agcagacaag
     2401 actcttatgg tcggagtttc tgaggagttg aagacacttg atttaacgga tatagcagga
     2461 acttaccctt atccaacctt actaatttgt ggcagcaaag ataaaccgaa tcttagttct
     2521 atgaaagctc tccatagact gttaacagat tcgcagtttc agatcattcc tgatggccct
     2581 catgtcttga ataaggcaaa accaaaggag tttgtagaaa agaccagaag tttccttgaa
     2641 ttgctgaaat aagtttgata aaataatatt gaaatcgata tcataattat gggagaaaga
     2701 aatgaataaa cgtctatttt caaaaatgag tctggtgacg ttgccaattt tagccttgtt
     2761 ttcacaatca gttttggcgg aagaaaacat ccatttttcg agctgtaagg aagcttgggc
     2821 gaatggctat tcggatattc acgagggaga acctggttat tctgccaagt tagaccgtga
     2881 tcatgatggt gtggcttgcg aattgaaaaa tgctcctaag ggtgctttta aagcaaaaca
     2941 gtcaacggct attcaaatca acacaagttc agcaacaaca agtggttggg ttaagcagga
     3001 cggcgcttgg tactactttg atggaaatgg aaatctagtg aaaaatgcat ggcagggaag
     3061 ctattacctg aaagctgatg gtaaaatggc acagagtgaa tggatttatg actcttctta
     3121 tcaagcttgg tattatttga aatcagatgg ttcttatgca aaaaatgcat ggcaaggagc
     3181 ttattacctt aaatcaaacg gtaaaatggc acaaggtgag tgggtttatg attcttctta
     3241 ccaagcatgg tattacttga aatcagatgg ttcatatgct cgcaatgcat ggcaaggaaa
     3301 ctactatttg aaatcagatg gtaaaatggc taaaggtgaa tgggtttatg atgccaccta
     3361 tcaagcttgg tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa
     3421 ttactatcta aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta
     3481 ttatgttggc gctgacggag tttggaagga agttcaagca agtacagctt cttctagtaa
     3541 tgatagcaat agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt
     3601 ccacatgtca aaaaaacgta tgtatagaca attaacttct gattttgata aattttcaaa
     3661 tgatgcagct caatatgcca ttgatcattt agatgattaa cagatactgg taggcgaaaa
     3721 aattccttat aattcattca cgcaaacctt tgcataagtg aatggatttt gatatactag
     3781 tactgtaagc attttcaatt actacacaat gaaaaaggag aatatgatga gtcaaaagat
     3841 tattgggatt gaccttggtg gaacttctat caaatttgca atcttaacaa cagcaggaga
     3901 aatccaagga aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga
     3961 tgatatgatt gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt
     4021 ccaaggcatt ggaatgggat caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg
     4081 tgcctacaac ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcttt
     4141 gggcattcca tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat
     4201 gggtgctgga gataaccaac cagacgttgt ctttatgaca ctcggtactg gtgttggtgg
     4261 tggtattgtc gcagaaggca aattgcttca tggtgttgct ggtgcagcag gtgaacttgg
     4321 tcatatcact gttgactttg accaaccaat ctcatgtact tgtggtaaaa aaggctgcct
     4381 tgagacagtt gcttcagcaa cagggattgt caacttgact cgtcgctatg ccgatgaata
     4441 cgaaggcgat gcagccttga aacgcttgat tgataacgga gaagaagtaa ctgctaagac
     4501 tgtctttgat ctcgcaaaag aaggagacga ccttgctttg attgtttacc gtaacttctc
     4561 acgttacttg ggaatcgctt gtgctaacat tggttcaatc ctaaacccat caacaatcgt
     4621 catcggtggt ggtgtgtcag ctgcgggaga attccttcta caaggtgttc aaaaagttta
     4681 cgatgaaaat agtttcccac aagtacgcac atctactaaa ttagctcttg caactctagg
     4741 aaatgacgct ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg
     4801 aaaatgctaa cctagagtta gtaatgttcc tcttttattt tttatgctat actagttagg
     4861 acaatgaatg aggtgaaacg aaatgacaaa agcagatacg atttttaaag agaatattga
     4921 acgcattcta aaggaaggtg ttttctcaga gcaggctcgt cctaagtaca aggatgggac
     4981 tgttgctaat tccaagtatg taactggtgc cttttccgag tacgacttat ctaaaggaga
     5041 atttcctatc acaaccttgc gtcccattgc aatcaaatcc gccatcaaag aagttctctg
     5101 gatttaccaa gaccagtcaa atagcttaga agtgcttaat gacaagtaca atgttcacta
     5161 ctggaatgac tgggaagttg gagacacggg aaccattggt gagcgctatg gtgccgttgt
     5221 taagaaacac gacattatca ataagcttct caaacagttg gaaaccaatc cttggaaccg
     5281 ccgcaatatt atttcgctct gggattacca agctttcgaa gaaacagatg ggctgctccc
     5341 gtgcgccttt cagaccatgt ttgatgttcg gcgtgttgat ggggaaatct atctggatgc
     5401 gaccttgacc cagcgctcca atgatatgct ggtggcccac cacatcaacg ctatgcagta
     5461 tgtggctttg cagatgatga ttgccaaaca ttttggctgg aaggttggga agttcttcta
     5521 cttcatcaac aacctccata tctatgataa tcaatttgaa caagctcagg aattgctccg
     5581 tcgggagccg tcaaactgcc aaccacgctt ggttttaaat gttcctgatg ggactaattt
     5641 ctttgatatc aaagcagaag attttgagtt ggtggattat gaccctgtta agccacagtt
     5701 gaagtttgac ctagctattt aaaagaatag aaaaaagaag ttgagaataa tcccaacttc
     5761 ttttgtttct taacgtgata cgcggcgacg agctgctttt ttacggtttt cttcgatgaa
     5821 agctgctttt tgctcttctg gttcgattac tttcttttta aatgcgtata ctgcacctgc
     5881 aacggcagcg acagttcctg cgacacctgt tacaagacct ttagcgaatc ctttagccat
     5941 gagtcttcct cctttatatt ctcaatcagc cagcctcctc aagaggtcac atttttctga
     6001 ctgacctttt tgtgttataa taatagtaac gaaaaaatgg gaatttttca aggaaaaaag
     6061 atgaaaacaa aaataattgt gattgttgga ccgactgctg ttggaaagac ggctctagct
     6121 attgaagttg caaagcgttt taatggcgaa gtggttagtg gagatagcca gcaagtctat
     6181 cgaggacttg atattgggac ggccaaggct agtccagaag agcaggcagc tgttcctcat
     6241 catttaatcg atgttagaga gataaccgag tcttactcgg cttttgattt tgtttcagaa
     6301 gctaagatga ctattgaggg tattcacaac cgtggcaagc tagccattat cgccggtggg
     6361 actggacttt atatccagag cttgctagaa ggttaccacc taggtgggga gactcctcat
     6421 gaggagattt tagcttatcg agctagtttg gagccatatt cagatgagga attagcccat
     6481 ttggtggatc aagcaggcct tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc
     6541 ttagaaattg cccattttgg tcaggatttg gaaaatcaag agaccttgta tgaaccgctg
     6601 attatctgct tggatgatga acgtagtcaa ctttatgagc gtatcaacca ccgagtggat
     6661 ttgatgtttg aggctgggct tttggatgag gccaagtggc tgtttgacca ttcccctaat
     6721 gtacaggctg ctaaaggaat tggctataag gaactctttc cttatttccg tggagagcag
     6781 accttggagg aagctagtga gagtcttaaa caggcgactc gtcgttttgc caagcgtcag
     6841 ctgacctggt tccgtaatcg catgcaggtc accttttatc agattggaga atctggtgta
     6901 caagaccgca ttttaagcca gatagaggag tttttagatg attgaaacgg agaaaaaaga
     6961 ggagcgagtc ctgctgattg gtgtggaatt gcagggtatg gacagttttg acctctccat
     7021 ggaagaattg gctagtttag cgaaaacggc aggggcagtc gttgtagata gctacagaca
     7081 aaaacgtgaa aaatatgatt ccaagacctt cgtcggctct ggtaagttgg aagagattgc
     7141 gcttatggtg gatgcagaag aaatcactac tgtcatcgtc aacaatcgtc tgaccccaag
     7201 gcagaatgtc aatctagagg aagttctcgg tgttaaggtc attgaccgta tgcagttgat
     7261 tttggatatc tttgccatgc gggctcgaag ccatgaaggg aagctccaag tccacctagc
     7321 ccaactcaaa taccttttgc ctcgcttggt tggtcagggg attatgctca gccgtcaggc
     7381 agggggaatt ggttcccgtg gtcctggtga aagccagctg gagctgaacc gtcgtagcgt
     7441 tcgcaatcaa atcacggata tcgagcgcca gctcaaggtg gttgagaaaa atcgtgcgac
     7501 tgtcagagaa aaacgtttgg agtctagcac ttttaagatt ggtttgattg gttatactaa
     7561 tgctgggaaa tcaactatca tgaacatctt gaccagtaag acccagtatg aagcagatga
     7621 gctctttgcg actctggatg cgacaaccaa gagtattcat ctgggaggca atctccaagt
     7681 aactttgaca gataccgttg gctttatcca agatttgccg acagagttgg tgtccagttt
     7741 caagtcaacc ttggaagaaa gcaagcatgt ggaccttctg gttcatgtta tcgatgctag
     7801 caatccttac cacgaggagc atgaaaaaac ggttctctcc atcatgaaag acctggacat
     7861 ggaagatatt cctcacttga cgctttataa taaagcggat ttggtggagg atttcacgcc
     7921 tacccaaacg ccatataccc tcatttctgc caagtctgag gacagtcgtg aaaacttgca
     7981 agcattattg ctagataaga ttaaggaaat ttttgaagca tttaccctgc gagtgccttt
     8041 ttcaaagtcc tacaagattc atgatttaga gagtgttgca attctggaag aacgtgatta
     8101 tcaggaagac ggcgaagtga ttacaggcta catttccgag aaaaataaat ggaggttaga
     8161 agaattttat gactgatatt aaaactttgg ctctaaagta tggaggttat acaagtctgg
     8221 acaaggtcta tctggatcag cttctagctg gtaaaacaga tcaggagcag ttaacactta
     8281 ttacacctcc gccaagtgtt gttaatgcct actttgcaga actctatcag aaaaagagtc
     8341 ctcaggctgc gacggattat tttgcagaac tcagtcagga actgaatctt tacaatactg
     8401 agccaagttt caccctagaa aataagcctt ttattcggct taatctgtct ggtaaatcct
     8461 ttggtttttg ttatgagagt gacggccttg gtcggatttt ctttgaaaat gaggagaaaa
     8521 tctcggatga cttgctcttt gaaattgcgc aaattttccc ccatcaatta gtctttgaag
     8581 agtctggtaa gatttacatg aagcctgtcg aggacgagga agttatcagc ctagaaagtc
     8641 tcacagcttt gactgatttg gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca
     8701 gccaagagga tttattgcaa gaagctgctg ctttttctgg caaacgctat ttccgatcgg
     8761 aaaaccgcac agccatgtta tatattgatt aattagaaag tatcgaatgg atattcaatt
     8821 tttaggaacg ggggctggtc agccctctaa agcccgcaac gtttcaagtc tcgccctgaa
     8881 acttttggat gagattaacg aagtttggct ctttgactgt ggagaaggta cgcaaaatcg
     8941 cattctggaa accacaattc gaccacgtaa ggtcagcaaa atctttatta cccatctgca
     9001 tggagaccac atttttggtt tgccaggttt cctttctagc cgtgcctttc aggccaatga
     9061 agagcagaca gatttggaaa tctacggacc tcaaggaatc aagtcatttg tcttaaccag
     9121 ccttcgtgtg tcaggttctc gtctgcccta ccgcattcat ttccatgagt ttgaccaaga
     9181 ttctctgggt aaaattcttg aaaccgataa attcactgtg tatgcagagg agctggacca
     9241 cactattttc tgtgttggct atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc
     9301 tgaaaaactc aaggctgctg gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg
     9361 ccaggatctt gttttggaag acggaactga aatcaaggca gcagactata tctcagcgcc
     9421 acgtccaggt aagattatca ctattttagg agacactcga aaaacgggtg ccagtgtgcg
     9481 tctggctgtc aatgcagatg tcctagttca tgagtccact tatggcaagg gtgatgaaaa
     9541 aattgctcgt aaccatggtc actcaactaa tatgcaagct gcacaagtag cggtagaagc
     9601 aggtgccaaa cgcctcctac tcaaccatat cagtgcccgt ttcctctcaa aagatattag
     9661 caaactcaag aaagacgctg ccacaatttt tgaaaatgtc catgtggtca aagacttgga
     9721 agaagtggaa atctagcagt cacagaaagg ataagtatgc ctactattct cattaccgga
     9781 gctagcggtg gtctagctca agaaatggtc aaactcctgc ccaatgacca actcatcttg
     9841 cttggtagaa ataaggaaaa attagcccaa ctctacggaa attattccca tgcagaattg
     9901 attgaaattg atattaccga cgattcagcc ctagaagctc tggtaactga tctttatctc
     9961 cgctatggca agattgatgt cttgattaac aacgctggtt acgggatttt tgagggattt
    10021 gaccagattg ctgataaaga tattcaccag atgtttgagg tcaatacctt tgccctgatg
    10081 aatctgtctc gtcaccttgc ggctcgtatg aaggaaagca gcaaagggca tatcatcaac
    10141 atcgtcagca tggcaggtct aatagctact ggcaagtcta gtctttactc agcgaccaag
    10201 tttgcggcta ttggtttttc aaatgctctg cgactcgaac ttatgcccta tggagtctat
    10261 gtgacaacag tcaatccagg accaatccgt acaggatttt tcgaccaagc cgacccagat
    10321 ggaacttatc ttaaatcggt tgatcgcttc ctgctagaac cagatgcagt ggctaaaaag
    10381 attgtcaaga ttataggaaa aaacaaacga gaactcaatc tcccgatttt gttgaaccta
    10441 gcccataagt tttatactct ctttcccaag ctagctgata agttggcagg ggaaactttt
    10501 aattataagt aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc
    10561 atttctcaac tatcaaactg aacttcttct aggagatagt ctataaagcg ttcgcccatc
    10621 ttagataggc tggttttctc atgctggata tagaccagct cgatcgggtc atcaatatcc
    10681 agtgggatag aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg
    10741 gtataaccat ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg
    10801 gatttcttgt ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc
    10861 ccttggtcat agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc
    10921 tttgccagag ggttggtctt gctgacaaag atatgcggtt gcgctgtgaa gagatggtgg
    10981 gctagcaggt gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag
    11041 aagaggaccc cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga
    11101 gtttcacgaa ggaagagttc gtatttctcc atatcgcttt tcttgagcaa agagacaaag
    11161 gcattgacca caaaggcata gtgttgagac gaaacgctaa agagttcgcg gtgggcgaca
    11221 ggatttttat agcgttcctc cagaagctgg gtctgctcga caacctgacg ggcataagag
    11281 agaaactcca tgccatcacg ggtcaaggtg attcccttgg gattgcggat aaagatctca
    11341 atgcccattt cattttccaa atctcgcact gcattggaga gacttggctg agtgataaag
    11401 agttgcttgg ctgcctcatt catggagcca gtttcgacga ttttgataat ataatgtaat
    11461 tgttgaattc tcatgttttt attgtaccat acttgcctta gaatcgctaa agaagatagg
    11521 aaaataagga gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga
    11581 gtcttatgaa aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt
    11641 acatgggaaa acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg
    11701 atgcagggtt gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc
    11761 acaaggtaga attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat
    11821 tttgtgggca cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg
    11881 ccgtgcggct aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt
    11941 gattgatttg cgcgacccag cagaattcca cagaaaacat atccttggtg cacgcaatat
    12001 tccttcaagt cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct
    12061 ctacgaaaac caacgtgcgc aacgagttac aaatgcagct ctttacttga aaaaacaagg
    12121 tttttctgag atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac
    12181 tagctaacct tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata
    12241 attaatttgt ataaaattta aggagttctt agaacataaa agaaaagaag aaaaatccac
    12301 tttttgtggg tatcttgtcg attattcttg gtttgttatt tccaattgta ggcctgattt
    12361 tgggtatcat aggattggtc ttggctattt cataccaaaa agagtcacaa ttagactata
    12421 aaatagaaaa gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag
    12481 caatcgcctt aatttttcgg taaataaaag aaaaaagctt tagttttaaa gctttttttg
    12541 ttttatcgac tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg
    12601 cggtagttac cttctgctaa atctgggttc aatgtgaagt ccccaaattg gattcttttg
    12661 aggctagtca ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct
    12721 tctgaaatgg tgatagaggc ttgactgagg gagggacttg cagatagaat ctctagtttt
    12781 gcgggtttac agacagtgtc atctaaaaag acaattcctt tttgaaaggt ttggatatgg
    12841 tcaggtgtta gaagtccatt aacctcaact tggtaagtct tatcgacatg atattgggga
    12901 tggaggagct gaaagcccaa gggaccgtta tcggtcaaga ggaggagtcc cgttgtatct
    12961 cggtccagtc ggccaacggc atagagcttg tcagactgga tgttagatgg aagcaggtcc
    13021 atgacggtcg gaagttcctt gtctttgttg gctgtaacgg caccagcagg tttatgaagc
    13081 ataagatagg tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt
    13141 cctgtatcga tattttgagc tagggagcgg gctgggcaac catcgactag aatttcccct
    13201 ctgaggagtg cttgcttcat ggcctttcgg ctgatttttt cttgagctaa taaattatct
    13261 aaacgcatac tgtgcctatc ttatcataag tcgctcgctt tgaaaaggct tgacgtgaaa
    13321 agaaaagcag agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg
    13381 gtataatcgt tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt
    13441 aagagaagat atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct
    13501 ggttgacgaa ttattgaaac aatcagaaac gcttgatgca cggactgaat tggcagagcg
    13561 tgctatggac tcaaacgata tcgaaaaaga gcgtggaatt accatccttg ctaaaaatac
    13621 tgccgttgct tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt
    13681 tggtggagaa gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc
    13741 ctatgaagga accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct
    13801 tgtcccaatc gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt
    13861 ggatgaagtc ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc
    13921 agtggtttat gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca
    13981 agaagcgact atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga
    14041 taactcagat gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg
    14101 acgtatcggt atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct
    14161 ttctaaactt gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg
    14221 tttggaacgt cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat
    14281 ggaagacatc tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat
    14341 cctacacatc gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc
    14401 tggtaaagaa ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt
    14461 gcaaacagac gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc
    14521 aggacgtgga gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga
    14581 acttcaagta tctcgtccag aagttatcgt aaaagaaatc gacggtatta aatgtgaacc
    14641 atttgaacgt gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct
    14701 ttctgaacgt aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt
    14761 ggtcttcctt gttccagcgc gtggtttgat tggatactca actgagttct tatcaatgac
    14821 tcgtggttac ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga
    14881 aattggtgga cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta
    14941 ctcaatcatg tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta
    15001 tgaaggaatg atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa
    15061 ggcaaaacaa atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac
    15121 gcctcgtatc ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga
    15181 agtaacgcct gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa
    15241 agctaacaag aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt
    15301 tagtcctagg aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag
    15361 ggactgtcaa tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc
    15421 tatcttttct gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttga
    15481 tcctagctta ctttagtgtt agagacatca cactcatgcc agtcaaaaaa agtaaaagaa
    15541 gataaaaaag agagtttcgg ctctcttttt gctaggaatt ttacaatagt ttgaattgct
    15601 ttttagttaa caccatctct tttacttgta tagtatgaat gggaaaaact tgaataaaat
    15661 tcgttaatgg catctatttt atatttggtt agtactggtt gcatattat
//
LOCUS       DAVHEW010000043        15288 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVHEW010000043 DAVHEW010000000
VERSION     DAVHEW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.043"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            35..850
                     /locus_tag="VVL53_001238"
     CDS             35..850
                     /locus_tag="VVL53_001238"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU4183414.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNTEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            869..1510
                     /locus_tag="VVL53_001239"
     CDS             869..1510
                     /locus_tag="VVL53_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU4183415.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKIIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1541..2542
                     /locus_tag="VVL53_001240"
     CDS             1541..2542
                     /locus_tag="VVL53_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU4183416.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSSTSIENLLANAQDIIR"
     gene            2552..3181
                     /locus_tag="VVL53_001241"
     CDS             2552..3181
                     /locus_tag="VVL53_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU4183417.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(3560..3945)
                     /gene="tnpA"
                     /locus_tag="VVL53_001242"
                     /pseudo
     CDS             complement(3560..3945)
                     /gene="tnpA"
                     /locus_tag="VVL53_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4329..7529)
                     /locus_tag="VVL53_001243"
     CDS             complement(4329..7529)
                     /locus_tag="VVL53_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU4183418.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTIGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESGNPYKVYVNDKEASLTEQEKDYPETQSVFLESFDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNRDSYGYML
                     IPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7752..8228
                     /locus_tag="VVL53_001244"
     CDS             7752..8228
                     /locus_tag="VVL53_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU4183419.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(8450..10663)
                     /locus_tag="VVL53_001245"
     CDS             complement(8450..10663)
                     /locus_tag="VVL53_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU4183420.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(11064..11240)
                     /locus_tag="VVL53_001246"
     CDS             complement(11064..11240)
                     /locus_tag="VVL53_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183421.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            complement(11619..12965)
                     /gene="celB"
                     /locus_tag="VVL53_001247"
     CDS             complement(11619..12965)
                     /gene="celB"
                     /locus_tag="VVL53_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU4183422.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NVVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            complement(13045..13551)
                     /locus_tag="VVL53_001248"
     CDS             complement(13045..13551)
                     /locus_tag="VVL53_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183423.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            complement(13582..13896)
                     /locus_tag="VVL53_001249"
     CDS             complement(13582..13896)
                     /locus_tag="VVL53_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU4183424.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(13906..>15288)
                     /locus_tag="VVL53_001250"
     CDS             complement(13906..>15288)
                     /locus_tag="VVL53_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU4183425.1"
                     /translation="FARIVLDECQEANLKLSDFVLQNLVVHIALSVIRLKSGFEIKNI
                     DCQMTDDAIERKVAQRILSKVREVTNQEFPVQEIDYITLHLLAKSQQCQKNQKNISEE
                     VLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLITHLMTLQVRLESRITLNNPLVDEIKQ
                     NYSDIFFMTREILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATG
                     FGAAQMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPVFK
                     VSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINKFENNGFRQYFSKENFLICTESDKVN
                     LLEKMVEGLSVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEGKVSVA
                     ICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNNFE
                     DFINIFEKIK"
BASE COUNT         4694 a         3192 c         2519 g         4883 t
ORIGIN      
        1 gagaagtctt aacaaatttg aaaggaagta tcaaatgaca aatacatcat tctcaattga
       61 gcagttttct ttaaaaggaa aaattgctct catcaccggc gcttcttatg gaattggatt
      121 tgctattgcc aaatcctacg ctgaagccgg cgctactatt gtctttaacg atatcaatca
      181 agatctggtc aataaaggga ttgaagctta tcgtgaagtt ggcatccaag cccatggata
      241 tgtctgtgac gtgacagacg aggacggtat ccaagccatg gtcaagcaaa tcgaacaaga
      301 ggttggtgtc attgacatcc tcgttaataa cgctggtatt atccgccgag ttccaatgtg
      361 cgaaatgagc gccgctgatt tccgtaaggt catcgatatt gacttaaacg caccatttat
      421 cgtttcaaag gcagttattc cttctatgat aaagaaaggg catggaaaga ttatcaatat
      481 ttgttcgatg atgagcgaac tgggacgtga aacagttagc gcttatgctg ctgctaaagg
      541 gggcttgaaa atgttgaccc gcaacattgc gtctgaatac ggtggagcca atatccaatg
      601 taacggaatt ggaccgggtt atattgccac tcctcaaaca gcacctcttc gtgaattgca
      661 agaagatggt tctcgccacc catttgacca gttcatcatt gcaaaaacac ctgctgcacg
      721 ttggggaaat actgaagatt tgatgggccc tgctgtcttt ctcgctagtg atgccagcaa
      781 ttttgtcaat ggccacatcc tatatgtaga tggcggtatc ttagcctaca tcggaaaaca
      841 acctgagtaa aaatagaaag aagatcttat gaaaatcgca ttaatcaatg aaaatagtca
      901 agctagcaag aatcacatta tttacgatag tctaaaagaa gcgacagata aaaaaggcta
      961 ccaattattt aactatggta tgcgtggaga agaaggagaa agtcaattaa cttatgtgca
     1021 gaacggacta atggctgcca tccttttaaa tacaaaggca gttgactttg ttgttaccgg
     1081 ctgtggtacg ggtgtagggg ctatgcttgc tttaaacagc ttccctggtg ttgtctgtgg
     1141 tctagcagtg gacccaactg acgcttacct ttattctcaa atcaatggtg gtaacgcctt
     1201 gtctatccct tatgccaaag gatttggctg gggggcagaa ctgaccctca aattgatgtt
     1261 tgaacgctta tttgctgaag aaatgggcgg tggctaccca agagaacgtg taatccctga
     1321 acaacgcaac gctcgtatct taaacgaggt gaaacaaatc acccacaatg atttgatgac
     1381 catccttaaa ataatcgacc aagacttcct caaagacacc atctctggca aatacttcca
     1441 agaatacttc tttgaaaact gccaagatga tgaagttgct gcttatttga aagaagtatt
     1501 agccaagtaa agctattcta aaccagaaag gaactaatgg atgacgaaaa tattactgtt
     1561 tggcgaacca ttaattcgaa tttcaccatt agatgccacc agtatcggcg atcatgttgc
     1621 cagttcgact tattttggcg gatcagaaat taacatcgct tgtaatttgc aagccctggg
     1681 tatctcaacg aaagttttta ccgcactccc tgccaacgag attggagatc gttttctcac
     1741 attcttgaaa cagcaccaaa tcgataccag ttcaatctgt cggcttggcg atcgaatcgg
     1801 cctctactat ttggagaacg gctttggttg tcgtcaaagt gaagttttct acgatcgtaa
     1861 gcatacgagt atcagccaga ttcggccaaa catgctagat atggattctc tctttcaggg
     1921 gattagccat tttcatttta gtggaatcac cgtagctatc ggtcaagagg tccgtgcgat
     1981 ccttctccta ctcttggaag aagccaagcg ccgaggaatt gtcgtttcaa tggatctcaa
     2041 tctgagaaca aagatgattt cagtcctaga agccaagtat gaattttcta agtttgcacg
     2101 ttttactgac tattgcttcg gtattgatcc tctcatgatt gatgaccaaa atctagagat
     2161 gtttccaaga gacagtgcta gcctagaaga ggtggaaaat cgcatgcgac ttttaaaaga
     2221 agcctatggt ttcaaggcca ttttccatac cctccgctct agtgatgagc aagacaaaaa
     2281 tgtctatcaa gcctatgctc tagaagaact atttgaagag tctgtccaac taaaaactgc
     2341 agtctatcaa cgaattggta gcggggatgc ctttatatct ggtgcccttt accaactact
     2401 ccatcattcc tccctaaaaa ctaccattga ctttgcagtt gcgagcgcaa ctctcaaatg
     2461 cactcttcca ggagaccatc tctccacttc ctcaactagt attgaaaatt tactggcaaa
     2521 tgcacaagat atcattcgtt aggagaatta catgaccaaa tcagatacga ttattgaact
     2581 aaaaaaacaa aaaattgtcg ctgttattcg aggaaataca aaggaagaag gactacaagc
     2641 ctcgattgct tgtatcaagg gcggtatcaa agctattgaa atcgcctata ccaatcagta
     2701 tgcaggacaa atcatcaagg aacttgtaga cttgtatcag gacgatcaga gtgtttgtat
     2761 cggtgcaggt actgtgcttg atgccgtaac tgctagagat gccattctag ctggagcaaa
     2821 ttacgttgtt tctccatctt tccatgctga aactgcgaaa atgtgcaatc tctacagcac
     2881 accgtacatt ccaggctgta ttaccctcac agagatcacg actgcacttg aagccggtag
     2941 tgaaatcatc aaactcttcc caggtagtac tctcagtcca gcatatatct ctgcagtcaa
     3001 ggcaccgatc ccacaagttt ccgtaatggt aaccggagga gtcggcctaa acaacatccc
     3061 tcaatggttc gctgctggtg cagatgccgt tggaattggt ggcgaactca ataaactcgc
     3121 ttcccaaggc aactttgacc gcatcagcga gattgcccaa cagtatatta cactcagata
     3181 aaatcataac tacccgtcta acgggtggtt tatctcagag ctataagccc aaatcatcag
     3241 ccagcgccta aagacgctgg ctttcacgtt gttcaagcct tattgctctt gactcgtcac
     3301 ttgcctcttt aagagacttt ggtattactt accactatcc ctaaagggat cctcatattc
     3361 ttttacactc aatttatcta gtgctatagt agattgaaac tggaatagta cacctctgct
     3421 tctaaaacat tgttaaaaat cgatttgact gtcctgatcg attttgtcct gttcttattt
     3481 cattttacta tatatcatac tttactcgtt ctcaaatttt catactcatg aagaaatcat
     3541 ccactcgata atttctttaa tcttgactat atttcttaat tgtggcttca ttaagcccta
     3601 ctggacttac ataataacct tcctcccaga aatgccgatt cccaaacttg tacttgagat
     3661 tggcgtgttt gtcaaacatc atgagtgcac ttttgccttt taaatacccc ataaaacttg
     3721 aaacacttag cctcgacgga atactgacta acatgtgtac atggtctggc attaagtgac
     3781 cctcgatcat ttcaacacct ttataactac acaagcgatg aaatatttcg tctaaactac
     3841 ttctatattg attatagatg acttttcgtc tatacttagg ggtgaacaca atatgataga
     3901 acacctccac tttgtgtatg ataaactatg agtcttttgt gccatatttt ttctcctttc
     3961 gctttacaat tggattgaac acctttattg tatcgcgttt ggagtttttt tggtataacc
     4021 ttcgacgcgc acccgtatag cgggtggttg ttttgtctcg cacctcacgg agcgagacgg
     4081 actaatatag tggagtgaaa taggatacga acaaattgat taggaaaatc aaatgaattt
     4141 atagaaatct tttagcagtt ataacgttct attctagttt caaaacgcta tagtcacata
     4201 ataatgaagt aaaaaaggat aagtatcaac ttatcctttt ttaaaagaaa aatccgaaga
     4261 tatttggcct tcttcggatt ttttctattt tccacagttt catgtaattc atctagatga
     4321 tgaacaaatt agttgttctt tcctctacgg aatagataaa atgccccaag tagcaagaac
     4381 cctagacttg ccaagattga ctgaccttct cctgtctgag ggagattctt ttgatccgaa
     4441 tggttctttt cctcttcaga tttttccttt tcttttgaat tctgtacttg tggctgagct
     4501 gcttgctcta gctttttaaa gacttcctga tctggagctg attcctgggt ttcaggatta
     4561 tagtaggcaa tcttatattc atccccttct tttcgaatgg tatagactcc acgtttcaaa
     4621 acttggaatt ggttggaaat agtagagaca gaatcatcat atttcacaat gccccaaact
     4681 ccttgtttag catcataaac agactgaagg gtttcgttat tttcgatgag gctactttct
     4741 aactctttta tcatttgatt gaaggtggca cgatccacgt taggaatgag catatagcca
     4801 taagaatctc tattttgctt atgagcctga ctaatcgtaa gaaattcatt ttcaacttcc
     4861 ttgtctgact gtccttcatt gatatccttc caggctccct tttgcaaagc cttactcata
     4921 ctgattgaac tcttcttaaa gaaaaagtaa ccaatattct ttttcgaatc gaacgattct
     4981 aaaaagacac tttgggtttc aggataatcc ttttcttgtt ctgtaaggga ggcttcttta
     5041 tcattgacat agactttata tggattacct gattccagtt ttctctggtc aattgtagtt
     5101 gcagcagtat ctgttgaagt gttttggata ttgcttccta aaaaggcgat cttatccttt
     5161 agcataaacc agctcttatg agcagtcaat gtttgattcc agttggtgaa atccatggtt
     5221 gctgtcgcat tggcatcatc tagtttgctc gttccaacga aagcagacgg taaaacttta
     5281 cctgtatcgc tatccgctct cttagcatcc gtctctgttg taccaggcat cttatatgga
     5341 ttaactgttg gccagtagcc atcgctatag tgactcaaat cgccattgta aagatagaac
     5401 atcccatcac tcgtatacca accacgttta ttttccttgt tcatgtgttc gtaattcaag
     5461 gtacgactgg aaaagagtga caagccaaat ccaaaccctt tctctgcatt gtacatggct
     5521 gttttatcca tcttgttaaa ggcagatagg taacttggtc ttggaacact tgcgactcct
     5581 gcatcactta acaaggattg catcaaactg atatccttat aagtcttcaa attcttaaag
     5641 acatcataat aactatccga ttgaacaatg gtcttcacaa gactctgcaa acgttgtttg
     5701 gtttctcctt cagacatatc cgctattcgg tgaatccctc ttagtacttc tactgcggcc
     5761 acgtgcccct cgctatttgc acgactgatc gagcgtccac gactcatatc catcaactct
     5821 ccattcacca gcaaaggagc aaacgattta tcaatccagt ggtacatggt ttgcatttta
     5881 tctttatcga ttggattctt ggtcttttga atgactggca acagttgaga caggccatca
     5941 atcaaaacat tcccataagc acccgtatag gcaacattgg tgtggtcgat ataggatcca
     6001 tcttgataaa aaccttcacc ttggtctacc aacttgaaca cttgctcaat cgagcgaatg
     6061 gtagaagaaa tttcttgatc atccttacgc agtaaaccag ctattacttt tacccttccc
     6121 atatcaacta agtttccacc tagagccttg aatgggttat cagtcgtctt tcggaaatgt
     6181 tcgggatctg gtacaaattt ttcaatcaca tctgtatatt ttttaatttc ctcatcagag
     6241 aagtattctt tcatcagaga caaggtattg ttgatggcac gaggtgtacc gatttcataa
     6301 tcccaccagt tcccaacaat gctcttttca ctattgtaga catgtttatg catccattcc
     6361 atggaatccc tgactgttcg aacgacagtt tcatcttgat aataacgaga agaaggattg
     6421 gtcacttgct tggccatctc ctccaatttc cgataagtgg cagtcagatt tgcagacgtt
     6481 ttataatttg aaaatttttc ccacaaatag gtgcggtccg cctgacttga aatactggat
     6541 aggctatcag ctacctttcc ttccaattcc tggtttaatt tggccatctg ttcattttta
     6601 gaatcatagt attgattccc agcgatgatg ccattccagt catccaaacg gtctgtgtat
     6661 gcatccttaa cagaggccag aatcttcaaa ggaatctttt tcacttcctt gccatcttta
     6721 ctgacaatga cattggttgt cccttcctta agaggttcta aaattccatt tttgactgaa
     6781 gcaacgtcag gattttctac cttataagta tagtccgcaa gagaaaaaac atgttttttt
     6841 ccaattggta aatcaatctt ttcctcaagc tgtttatctg tttgagaatc ctcagaaagc
     6901 tggtctgcta cctctaccag ctcaatatcc ttaaaggaaa cagtcccagt tcctgtttca
     6961 tagaataact ccagcttgat tttatcaaca tctaaagtcg ggctatagtc tgcttcaatg
     7021 gtctgccagt cctttgttcc tgacgtcgtt gcagaattcc acaatcgctt gtccttacca
     7081 ctttcctcaa tgatacgaac tttggcaatc ccgattttat tatctgtttt aatcttgaaa
     7141 cgcagtttat actttttctt agcttcaata ggaaccatac ggtgaagcgc tgcccttaat
     7201 ttctcatggc ttgagatagt gatagcccca tccttagcct caatgactcg agttgaggca
     7261 tctgcactat tcttctggtc tacccaagct gaccacccct gagctttgct tcctgtccac
     7321 ggactgcttc cgtcctccgt ctgaccaaaa tcaccatttt caactaaatt ttttgtatcc
     7381 ctttgctgat actgaacttc cttctggctt tgctgaatgg tattcgtagt cgtttcttcc
     7441 gctccaattg tcatataatg gtttaataaa aatcctgata aaacaagtga agacaaactc
     7501 acaatgagct tctttgtttt tgtttgcatg tatacactcc aataaacata ttgtaaaatc
     7561 aataggaact aaatctcaaa ttagagattt atagcaacta agtaaaccgg tttaattaaa
     7621 tcataccatt tttaaagtta tttgtaaacg gtttctatca tattgaaggc ttattcttca
     7681 acaatatctt ccatccgact cgacgaaaaa agtaaaaact gttacaatgt agaaaaagaa
     7741 aagaggccct catgacttca ctttatgatt tttccgtttt gaaccaaaac aaccaagcaa
     7801 ctcccttgga tagctatcgt ggcaaagttc tcttgattgt caacactgct actggatgtg
     7861 gtttaacgcc ccagtaccag ggacttcaag aactctatga acgctatcaa gatcagggct
     7921 tcgaaatatt ggatttccct tgcaatcagt ttatgggaca agcacccggc agcgcagagg
     7981 aaatcaacgc cttctgtagc ctacattttc aaaccacctt cccacgtttt gccaagatta
     8041 aggtcaacgg taaggaagca gaccctctct atgtctggtt acaagaccag aaatccggcc
     8101 cactaggaaa acgagtcgaa tggaatttcg ctaagtttct catcggtcga gatgggcaag
     8161 tctttgaacg cttttcttca aaaacagacc caaaacaaat tgaagaggcg atacaaactc
     8221 tactataatt cacaatctca ctatgattag gtttccttta acctgatgaa tagtgagatt
     8281 ttttgatggg ctttgactta aatagaaaaa caccccatga tatgaaacat gaagtgttgt
     8341 aaagtctatg ttgtaggtgc ttatttcaca atttcaatgt gaccagtgat aacgaatacc
     8401 atacagaatc ttcatataca ctaaacaaat gactttctaa ttatttcaat tagttttggc
     8461 tagtaaatat catttccaac aaacgccctc tcaattcctt atcctgatga tgcaagatat
     8521 tcattaagtc atgagagttt ttcgcattga tgaattgatt taacaatcta tcttttaatt
     8581 catatggaag agaagctgtc tttagtagtc taaaaacttc gtcatttaaa gatgtccttt
     8641 tattatcttt ccattcaaat ttagctgtat cattcttatt tggcaattca attatagaca
     8701 cattcgttcc tttaaaatga attctatgtt ttctattgct tggaacgata ctagaatctc
     8761 cttgtaatgc taactctacc attcccattt cccaatcgat tgataatctt gttttatatc
     8821 tttgaccatt ttgatcttca agcatttcaa aagaatgttg ttttcctggg aatacatacc
     8881 aatctacaac ttcaggtaaa tcaacaccca tacctatctc agaaccaacc aagggaatga
     8941 ttgcaccact ttttgcaaac acaggcgtag tcgagatgtc cctataaaca cttaacttca
     9001 caccacctgt gtattttttc tctgaaaaga agtcatacca ttcaccttca gggaaccata
     9061 catctacttt tgcagattgg aatgtcaaat ccatcttttc tacaatggga gccaccatca
     9121 gttctgttcc aaaaaagtat tggtttggaa cattatagct ctcatcattc tctggataga
     9181 aataatagat tggactgatt aatggggcac cttcctcatg tgtctgtaca ttcatggtat
     9241 atagataggg aatcatctga tgtctcaaac gaaggtattt cttcataatc ttagatgttg
     9301 tttctgaaaa aaaccaaggt tctttactat taaaaggact tctagaacta tgtaatcgag
     9361 taatcggact aaaaacacca aactgtagcc atctagtttg tagctcttcg tcataatccc
     9421 ccaacatatg tccaccgata tcatgactcc accaactata accgatatta gatgctgtcg
     9481 ctgtaaaata gggttgaaat cttaaggaat tccaactaat aatagtatcc cctgaaaaac
     9541 caacagggta gcggtgacta ccaggacctg catatcttga taaaatcaaa ccaccttctg
     9601 catttttaca actatcctga tagtgataat ggtttaaaag ccaaagtgga tctagcatac
     9661 cttgtgtccc ttgttgccag tcaatccacc aaaaatctac tccctgcttt tctagttcat
     9721 aatgaacatc tttaaagtag gcttccctaa aagagggatt aaaaaaatca aaaatagcag
     9781 gttcttctag ttctacattt aaccccaacc gttttgcgat ttgaggataa gcttcttcat
     9841 aagcccgtat cccatcagca ggatggacat ttaaggagag ttttagcttt ctatcatgaa
     9901 gttgttgcaa taactgttct ggatttggta ttaagtttct attccaacta tatcctgtcc
     9961 agccacttcc aaagcgagct ggaatgtcag ttatatgcca atccatatct aacacaccga
    10021 tagataatgg aattttctct gtttcaaatc tgtctattaa atccaagtat tcatccgacg
    10081 tataaggcca atatctactc caccaattgc ctaaagcata tcttggcaac aagggtgttg
    10141 aaccagtcaa atggtaaaaa tctctgattg ctcctctata atcatgccca taggcaaaga
    10201 aatacaggtc aatttgattt tctctctcaa tataaccaga ttgttcatcc caaataaatc
    10261 cttgagaatc atccaataag gctataccat ttcggctaat aattccatct tctaacgaga
    10321 ttgctccatc tgccttatcc agagtccgag ctgttccttt taacgtttca atagattcac
    10381 caaaatacca gcgactacca tatacggcaa aatttccttt taattctata aataaatttt
    10441 cggcgttaaa ttctccttta ttaaagtgca gatgaaaata gtccgtcata atatctagta
    10501 cgtttgatgt ctcgatataa tctaacgaaa tttggccaaa atctctatta tagataagtt
    10561 gtgtcgttct atcctcaaaa cttccagttt gagagtattc taaccttact agcttgtctg
    10621 ttaatacaga gattcgataa aactctccct taaaaatttt caatttgttt tcctcctttt
    10681 atggtagcat aaaaacagaa cgcaccattt ttgatgcgtt tttcattatt ctgaatgcaa
    10741 tgttctatct gttatatcta tgacaaataa tagtcaattg aaaaaatgca gtggacaaaa
    10801 tatcttttaa caaaccaaga gtttattaaa gagttatcac ttttcaactt ttctaagctt
    10861 atgcagttgt gaaacaaact acttttaaac tattaactaa gataggattg ataaataatt
    10921 tcaaactctt actagcaatc atacgatatt caagctcacg tgcttttttc cttcctgctt
    10981 atttcttaga actgaagaac ccggatcggt atataaatta tccggatcaa catagtcata
    11041 agattcataa cagttgcgct tcattaagtc atccccagag caagagcttc atctcgtaat
    11101 ttttcaacat cactaaccgt aggtcgccat ccttcaatca tatttgtact taaagcatac
    11161 caaacactct taaaaacgga tcggttttca aaagctattc ccatgattgt catcttttct
    11221 ttatctatat ctaaggacat atgctacctc ctttagatac attataccat gtttctctgt
    11281 agcttttaaa aattttattt tgtttgtcat atctaagttt tcagcacgct tatcctattt
    11341 tataagcctc aaacccaaat ataaaacgca ttctttttgc ttttttacta ttgtatcgta
    11401 ttctacgata acatacttta ctttattgtt tttttaaata acagcagttc cctgtttatc
    11461 aactattcga actactttct attttgcttc ataccctaca tagcgaaaaa atatgaaaaa
    11521 gcagagaaga atatcttaaa aagacctctt cactgctaat attaacactc attatttaaa
    11581 ctatatggat tctatcatcg agtatacttt tttacttatt agataccttg ctcttctttc
    11641 accaattttt gatcatatac acggatgaat ggaagataga ctaggaatgc tgcaaatgca
    11701 catactagag caactaatac agctcgaaga tctgctgtcc ctaagaaagc tccaatccct
    11761 actggagttg gccatggaac ctgtgcgata attggcttaa taaagtttag agaattcgct
    11821 acgtaataaa tagtagcagt aaccattggt gctaaaataa atggtatagc caaggctgga
    11881 ttatagataa taggtaatcc aaaaattaat ggttcattaa tattaaataa ggctggaact
    11941 acagatgctc gtcctattgc tttaagctgt tcagatttag aggcaaaagc aatatataaa
    12001 catagtccta aagttgcacc agaaccacct gcaattacaa acatattaga aaattcacct
    12061 gcaacagcga agtgcccgcc agcagcattt tcagccatgt tagcaagagc aattggacta
    12121 acaaatgcaa aaacaatgtt cgcaccgtgg atacctacaa tccaaagtag ttgagtcaat
    12181 agataaataa tcattaaacc aatccacgaa ttagtcagat tggatacaaa accaaatgga
    12241 attgcaatga ctttaaaaat atctgttccc attgctacaa gaagaccgtt gataaagata
    12301 acaacaaatg caacaacaaa tcccggaacc aaagcggtaa atccacgaga aactccttct
    12361 ggaacagctt caggcatttt aataacccaa ttatgtttaa cacacatacg ataaataaga
    12421 acagtcacaa ttgccataat gattgcggta aaaatccctg ttgtcccaaa acgtgcgact
    12481 acatttccca ttgcccatcc atctgcaatt actgcacctt cttttagact tgtcacagtc
    12541 ttcatcattc caccatcaaa aatgatttgc ggtactgtca tgacaaaagc catcaaggca
    12601 agcaaggcac cattaagagg attcatattg agttcttctt cctctgcata aatttttgtc
    12661 aattcatatg caagtgatag aacgaaataa agagatagag aacccatagt cgcatagttt
    12721 gcaaccatgt aaagtgatgt gaatttatca aatgaagcag agaaaatatc tgccacaatt
    12781 ggccaaaatg agaaagcttg tggcaaaata ctgaatacca aaaacattga tcctacaata
    12841 gtaaatggta cagcagccat acctgcagcc gtgatagcac gtactacttt aaactgagca
    12901 agtttgccca ttggtcccat aacatggttt tcaagaaaac caaacaaccc gttttgttga
    12961 tccataaata gacctcctta ataaaacata ataattttta ctttctaaag actagtttca
    13021 aatacaaatt atactagatc aggattataa actaagtgag ttcttttcca attggacaaa
    13081 ttgttgataa gccttatctg ttcgtttata aattttttta attcttctaa tgtctaacaa
    13141 actcagaact aaacctaata gaagaactac aaaaacaaat aaacgtgcta cttggttatt
    13201 ttcaaaaatc ggaaaaagat tcttaaacca acttgtccaa gttaaaacaa gtaatcctat
    13261 tgaaataagc atttgtattc taacaaacat tagtgttatt cccaactttt ctttcctatt
    13321 tccataaagt ttaaattgtt caatagttgc taaaatagaa aatactatga gcataatggg
    13381 gaaaataata ataggcgagg gattaataaa ctgattcaaa agccaataaa tattcccaaa
    13441 aaagaagagt gctattgaat aacgtagaag aagatatcga ttaaaaaaag tattagttag
    13501 agccatctct cgacgttgtt gttcaatttt ttgtcgttct tttttatcca tatcatttcc
    13561 tccttatata acaacacata tttagttaac tttcttataa agagctaaca tttcctttgc
    13621 tacttctaat aatgtcatag tggtcattaa atgatcttga gcatgtacca tgataatttc
    13681 aattttaatt tccactccac ttgcgtattc ttgcaagagt ttggtttgtg catgatgcgc
    13741 ttcaagaatt atctcatttg attgatttaa tttactttct gcatcatcaa aactaccttc
    13801 tctcattttt gcaaatgctt catgtatttc tgaccttgca tttcccgaat gcaggataat
    13861 ttcaaatgct gcaacctgca gttcctcttg attcatatga acctcctatt ttatcttctc
    13921 aaatatgtta ataaaatctt caaagttatt gcaagatatt agctgatttt gcaattcatc
    13981 attctctgtc agagagacta tctttttagt cacagttgct aaaccttcgt tcccatatat
    14041 tgatggagat agaagaaata ctagctggac atgtgaactt tgattatccc agagtaacga
    14101 atctttacaa attgcaaccg aaacctttcc ctctgtacca aagggctgaa taggatgcgg
    14161 aactgcaatt ttttcagaaa aaacaactga acttaattct tcgcgctgtt taattccata
    14221 aagtaaagat tgttcaaact catttgattc accaacagat aaaccctcaa ccatcttttc
    14281 aagtaaattt accttgtctg attcagtaca tattaaaaaa ttttctttac taaaatactg
    14341 tctaaagccg ttgttttcaa atttgttaat ctttgatgat tgtacataac tagaaacttg
    14401 catctgatcc atagcttttc taatcatttc catctcatca cttttaagaa acacactaac
    14461 tttaaaaact gggatttgaa aatatagatt tgataaatca acagctgaca ctataaaatc
    14521 tattccttta agtttttctt gattcaattc atagtagcct attacatcaa caacttctac
    14581 tcgcttccca aactccgttt ccaaacgatt tcttaacatt tgggctgcac caaatcctgt
    14641 tgcacaaata gcaagaatat taaacttagt actctctttg ctacgttcca tagcagctaa
    14701 aaagtgaaga cttacatatg ctacttcatc atctgatatt gtccactcca agaacttgtc
    14761 catatttgca agaatttctc tagtcataaa gaatatatca ctataattct gtttaatttc
    14821 atctaccaaa gggttattta aggtaatccg gctttctaaa cgtacttgta gtgtcattag
    14881 atgagttatc aatccttcaa ttagttggaa atctgaagaa aagttataca tatcatctaa
    14941 tcctaaattc tgaaatgttt taaataaaga tttttttaaa acttcttcag aaatattctt
    15001 ctgatttttt tgacattgtt gactcttagc taacaaatgc aaagtaatgt agtctatttc
    15061 ctgaactgga aattcctgat ttgttacttc tcttacttta gaaagaattc tttgggcaac
    15121 ctttctctct attgcatcat cagtcatctg acagtctata ttttttattt caaatccgga
    15181 ttttaaacga atcacagaca atgctatgtg aactactaaa ttctgtagta caaaatcaga
    15241 tagttttagg ttggcctctt ggcattcatc caaaacaatt ctagcaaa
//
LOCUS       DAVHEW010000044        14894 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVHEW010000044 DAVHEW010000000
VERSION     DAVHEW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.044"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..156)
                     /locus_tag="VVL53_001251"
     CDS             complement(<1..156)
                     /locus_tag="VVL53_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4183426.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGT"
     gene            522..1262
                     /locus_tag="VVL53_001252"
     CDS             522..1262
                     /locus_tag="VVL53_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU4183427.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1259..2170
                     /gene="pfkB"
                     /locus_tag="VVL53_001253"
     CDS             1259..2170
                     /gene="pfkB"
                     /locus_tag="VVL53_001253"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU4183428.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2167..4119
                     /locus_tag="VVL53_001254"
     CDS             2167..4119
                     /locus_tag="VVL53_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4183429.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4283..6508
                     /locus_tag="VVL53_001255"
     CDS             4283..6508
                     /locus_tag="VVL53_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU4183430.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(6553..6981)
                     /locus_tag="VVL53_001256"
     CDS             complement(6553..6981)
                     /locus_tag="VVL53_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183431.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7119..8261
                     /locus_tag="VVL53_001257"
     CDS             7119..8261
                     /locus_tag="VVL53_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4183432.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8270..9484
                     /gene="thiI"
                     /locus_tag="VVL53_001258"
     CDS             8270..9484
                     /gene="thiI"
                     /locus_tag="VVL53_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU4183433.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            9619..10443
                     /locus_tag="VVL53_001259"
     CDS             9619..10443
                     /locus_tag="VVL53_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4183434.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            10465..11204
                     /locus_tag="VVL53_001260"
                     /pseudo
     CDS             10465..11204
                     /locus_tag="VVL53_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11217..12383
                     /locus_tag="VVL53_001261"
     CDS             11217..12383
                     /locus_tag="VVL53_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4183435.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            12891..14384
                     /locus_tag="VVL53_001262"
     CDS             12891..14384
                     /locus_tag="VVL53_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU4183436.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            14397..>14894
                     /locus_tag="VVL53_001263"
     CDS             14397..>14894
                     /locus_tag="VVL53_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4183437.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDEL"
BASE COUNT         4447 a         2788 c         3319 g         4340 t
ORIGIN      
        1 tgtacctctt ttgacttcat tatgaatcgt ttgaggcgcc tttcctaaca agcctgcaat
       61 ttcacgattg gacttatttt cattcttcca ccgctcaatc aagcgacggt tatctattgt
      121 caaatgtttc ccttttgggg tataatgttc ttgcatctct gtgcctttcc ttgtgtttgt
      181 ggtgaacaac aagtatagca cagaggtgtt ttcttatacc ttaaatcaca tttcatttga
      241 caacaggaac ccttgaacct gttttttcag ctaaaccaag gctatgatta gccttggcca
      301 cctagcctaa cagtttcaaa cctgttatca aatgaaatca tcaacttact caaacatagt
      361 gaaacatcta attcttgact aaggtaaggt ggctaacttc attatagaac tttcaaaata
      421 agaaaaaatg atagattttg aaagaaaatg ataaaaaatg cttgacttcg aaagaaagta
      481 tgatagaatg aatagtgtaa acgataacag gaggtgattc agtgttaaaa acagaacgta
      541 aacaactaat tttagaggag ttaaatcaac atcatgtagt ttctctagaa aaattagtta
      601 gtttgctaga aacgtcagaa tcaacggttc gaagagactt ggatgagttg gaagcggaaa
      661 acaagcttcg tcgtgtgcat ggtggagcag aactccccca ctccttacag gaagaagaaa
      721 ccattcaaga aaaatctgtc aaaaaccttc aagaaaagaa gttgctggct cagaaagcag
      781 cctctctcat taaagaaaaa gatgtcatct ttatcgatgc tggaacaaca actgcttttt
      841 tgattcatga attggtcaat aagaatgtta cagttgtgac caactccatt caccatgccg
      901 ctcagttggt tgaaaagcag attccaactg tcatggttgg aggaaacgtc aagacggcga
      961 cagatgctag tatcgggggc gttgctctta accagattaa ccaattgcac tttgaccgtg
     1021 cctttatcgg aatgaatggt gttgacgatg gctattatac gactcctgat atggaggaag
     1081 gagctgtgaa aagagctatt ttggagaatg ccaagcagac ctacgtcttg gtggattcgt
     1141 caaaaattgg acaaacttgc tttgccaagg tagccccact caaacgcgct atcgttatca
     1201 ctagtcaagg gcatgagctc ttgcaggtta ttaaggagaa aacggaggta atagaagtat
     1261 gatttataca gtcacactca atccatccat tgactatatc gttcgtttgg accaagtcaa
     1321 agttggtagt gtcaatcgta tggacagtga tgataagttt gctggtggga aaggaatcaa
     1381 tgtcagccgt gtcttgaaac gtttgaatat accaaataca gcgacgggat ttatcggtgg
     1441 ctttactggt aaatttatca cagatacttt agcagaggaa gaaatcgaga cacgttttgt
     1501 ccaggtggca gaagatactc gtatcaatgt taaaatcaaa gcagaccaag aaacagaaat
     1561 caacggaacg ggtccaactg ttgaatcggt tcagctagaa gaattgaaag ctattttatc
     1621 tagtctgaca gcagaagata cagttgtctt tgcaggttca agtgctaaaa atctaggcaa
     1681 tgttatctat aaggatttga tttccttgac gcgccagact ggtgcgcaag tggtctgtga
     1741 ctttgaagga cagaccttaa ttgatagttt ggactaccag cctcttcttg taaaaccaaa
     1801 caatcatgaa cttggagcga tttttggggt taaactcgaa agtttagatg aaattgagaa
     1861 atacgctcgt gagttactgg ctaagggtgc tcaaaatgtt attatctcta tggctggtga
     1921 tggtgccctt cttgtcacat ctgagggagc ttacttcgct aaaccaatca aaggaacagt
     1981 caaaaattca gttggagctg gtgattctat ggttgctgga ttcacaggtg aatttgtcaa
     2041 atcaaaagac gtagtagaag ccttcaaatg gggagtggct tgcggaacgg caactacctt
     2101 ctcagatgac ttggcaacgg cggaatttat taaagaaaca tatggaaaag ttgaggtaga
     2161 aaaacgatga aaattcaaga cctattgaga aaagatgtca tgttgctaga tttgcaggca
     2221 actgaaaaaa cagctgtcat cgacgagatg attaaaaatt tgacagacca cggttatgta
     2281 acagattttg aaacatttaa agaaggaatt ttggcgcgtg aagctttgac ttctactggt
     2341 ttgggtgatg gaatcgcaat gcctcacagc aaaaacgctg ctgtcaaaga agcgacagtt
     2401 ctatttgcta agtcaaataa gggtgttgac tacgagagct tggatggaca agcaactgac
     2461 ctcttcttca tgattgcagc tccagaaggt gccaatgata ctcacttggc agccttggca
     2521 gaattgtctc aatacttgat gaaagacggt tttgcagaca aacttcgtca agcaacatct
     2581 gcagaccaag ttatcgaact ttttgaccaa gcttcagaaa aaactgagga acttgttcaa
     2641 gcacctgcta atgactctgg tgactttatc gtagctgtta cagcttgtac aacaggtatt
     2701 gcccacactt acatggccca agaagccctt caaaaagtag ctgctgaaat gggggttggt
     2761 atcaaggtcg aaaccaacgg tgctagcggt gttggaaatc aactaactgc agaagatatc
     2821 cgtaaggcta aagctattat cattgcagca gacaaggccg ttgaaatgga tcgatttgat
     2881 ggaaaaccat tgatcaatcg tccagttgct gacggtatcc gtaagacaga agagctaatt
     2941 aacttggctc tttcaggaga tactgaagtc taccgtgccg ctaatggtgc caaagctgca
     3001 acagcctcta acgaaaaaca aagccttggt ggtgccttgt acaaacactt gatgagtggt
     3061 gtatctcaaa tgttaccatt cgttatcggt ggtggtatca tgattgccct tgccttcttg
     3121 attgacggtg ctttgggtgt tccaaatgaa aaccttggca atcttggttc ttaccatgag
     3181 ttagcttcta tgttcatgaa aattggtgga gctgcctttg gtttgatgct tccagtcttt
     3241 gcgggttatg ttgcctactc tattgctgaa aaaccgggtt tggtagcagg tttcgtggct
     3301 ggtgctattg ccaaagaagg ttttgccttt ggtaaaattc cttatgccgc aggtggtgaa
     3361 gcaacttcaa ctcttgcagg tgtctcatct ggtttcctag gtgcccttgt tggtggattt
     3421 atcgcaggtg ccttggttct tgccatcaag aaatacgtta aagttcctcg ttcactcgaa
     3481 ggtgctaaat caatccttct attgccactt cttggaacaa tcttgacagg atttgttatg
     3541 ctagctgtga atatcccaat ggctgcaatc aacactgcta tgaatgactt cctaggcggt
     3601 cttggaggag gttcagctgt ccttcttggt atcgtccttg gtggaatgat ggctgttgac
     3661 atgggtggac cagttaataa agcagcttat gtctttggta caggtacgct tgcagcaact
     3721 gtttcttcag gtggttctgt agccatggca gcagttatgg ctggaggaat ggtgccacca
     3781 cttgcaatct ttgtcgcaac tcttcttttc aaagataaat ttactaagga agaacgtaac
     3841 tctggtttga caaacatcat catgggcttg tcatttatca ctgagggagc gattccattt
     3901 ggtgccgctg acccagctcg tgcgattcca agcttcatcc ttggttcagc agtagcaggt
     3961 ggactcgttg gtcttactgg tatcaaactc atggcgccac acggaggaat cttcgttatc
     4021 gcccttactt caaatgctct cctttacctc gtttctgtct tggtaggagc aatcgtaagt
     4081 ggtgtggttt atggttacct acgcaaacca caagcataaa aaatagaaaa atgaaaagat
     4141 tggaccgttt ggtgcagtct ttttctcttc ccgaaatgcc tgtgaaatat ggtataatag
     4201 aagaatggca aacaagaata caagtacaac aagacggaga ccgtctaaag cagaactgga
     4261 aagaaaagaa gcgattcaac gaatgttgat ttcgttagga attgcgattt tattgatttt
     4321 cgcagccttc aaattagggg ctgcaggtat aaccctttat aatttaattc gcttgctagt
     4381 gggtagccta gcttatctgg cgatattcgg cctattaatc tatctcttct ttttcaagtg
     4441 gatacgaaaa caggaaggac tcttatctgg ctttttcacc atatttgctg gcttactctt
     4501 gatttttgag gcctacttgg tttggaaata tggtttggac aagtccgttc taaaagggac
     4561 catggctcag gttgtgacag atctgactgg ttttcgaacg actagctttg ctggaggggg
     4621 cttgatcggg gtcgctcttt atattccaac agcctttctc ttttcaaata tcggaactta
     4681 ctttattggt tctatcttga ttttagtggg ttctctccta gtcagccctt ggtctgttta
     4741 cgatattgct gaatttttca gtagaggctt tgccaaatgg tgggaagggc acgagcgtcg
     4801 aaaagaggaa cgctttgtca aacaagaaga aaaagctcgc caaaaggctg agaaagaggc
     4861 tagattagaa caagaagaga ctgaaaaagc cttactcgat ttgcctcctg ttgatatgga
     4921 aacgggtgaa attctgacag aggaagctgt tcaaaatctt ccacctattc cagaagaaaa
     4981 gtgggtggaa ccagaaatca tcctgcctca agctgaactt aaattccctg aacaggaaga
     5041 tgactcagat gacgaagatg ttcaggtcga tttttcagcc aaagaagccc ttgaatacaa
     5101 acttccaagc ttacaactct ttgcaccaga taaaccaaaa gatcagtcta aagagaagaa
     5161 aattgtcaga gaaaatatca aaatcttaga agcaaccttt gctagctttg gtattaaggt
     5221 aacagttgaa cgggccgaaa ttgggccatc agtgaccaag tatgaagtca agccggctgt
     5281 tggtgtaagg gtcaaccgca tttccaatct atcagatgac ctcgctctag ccttggctgc
     5341 caaagatgtc cggattgaag caccaatccc tgggaaatcc ctaatcggaa ttgaagtgcc
     5401 caactccgat attgccactg tatctttccg agaactatgg gaacaatcgc aaacgaaagc
     5461 agaaaatttc ttggaaattc ctttagggaa ggctgttaat ggaaccgcaa gagcttttga
     5521 cctttctaaa atgccccact tgctagttgc aggttcaacg ggttcaggga agtcagtagc
     5581 agttaacggc attattgcta gcattctcat gaaggcgaga ccagatcaag ttaaatttat
     5641 gatggtcgat cccaagatgg ttgagttatc tgtttacaat gatattcccc acctcttgat
     5701 tccagtcgtg accaatccac gcaaagccag caaggctctg caaaaggttg tggatgaaat
     5761 ggaaaaccgt tatgaactct ttgccaaggt gggagttcgg aatattgcag gttttaatgc
     5821 caaggtagaa gagttcaatt cccagtctga gtacaagcaa attccgctac cattcattgt
     5881 cgtgattgtg gatgagttgg ctgacctcat gatggtggcc agcaaggaag tggaagatgc
     5941 tatcatccgt cttgggcaga aggcgcgtgc tgcaggtatc cacatgattc ttgcaactca
     6001 gcgtccatct gttgatgtca tctctggttt gattaaggcc aatgttccat ctcgtgtagc
     6061 atttgcggtt tcatcaggaa cagactcccg tacgattttg gatgaaaatg gagcagaaaa
     6121 acttcttggt cgaggagaca tgctctttaa accgattgat gaaaatcatc cagttcgtct
     6181 ccaaggctcc tttatctcgg atgacgatgt tgagcgcatt gtgaacttca tcaagactca
     6241 ggcagatgca gactacgatg agagttttga tccaggtgag gtttctgaaa atgaaggaga
     6301 attttcggat ggagatgctg gtggtgatcc gctttttgaa gaagctaagt ctttggttat
     6361 cgaaacacag aaagccagtg cgtctatgat tcagcgtcgt ttatcagttg gatttaaccg
     6421 tgcgacccgt ctcatggaag aactggagat agcaggtgtc atcggtccag ctgaaggtac
     6481 caaacctcga aaagtgttac aacaataaaa aaatagcttc tttccaagtt tggagggaag
     6541 ctattttagt ggctattgat tgcttttatt ttctgaagtt ggcgcattgg actgtttttc
     6601 gttttcagta gcaggtttac ttgaagcagg agtagaagag tcctgagttg ctgttttctg
     6661 atcttctttt ttctcttcct tgacgctaga ttttggtgtt tcctcttgct gtgttttttc
     6721 ttgactagtg ttagtctctt tagttggact ggtgttttcc ttaggggatt ccttttggat
     6781 ttctttgaca atggttgtcg tctggcttgt cgtaggttct tttttaatat ttttgttatt
     6841 atccaaggcg ttcataatcg tgatacttgc ggtaatcaag ccaaggatac tagcgattgt
     6901 agcaacggtt ttttgaaaga ctgtaaagcc ttttttgatg tgttctgttt ttggttttga
     6961 agttctacga tagttagaca tacattctct cctttgatta tgatttatta tactctgttt
     7021 ctttaaaaaa atcttaaaaa aagagaaatt tccctttctt gtcgcaagct ccgtttcatg
     7081 atacaataga ggaagtgatt ttagaaagcg tgagaaacat gatttacttt gataattcag
     7141 cgacgaccaa gccttatcct gaagcccttg aaacctatat gcaggtcgct tcaaaaattt
     7201 taggaaaccc atctagtctc catcgtttgg gagaccaagc aacacgaatc ttagatgctt
     7261 ctcgccaaca gattgcagat ttaatcggta agaaaagcga tgaaatcttc tttacctcgg
     7321 gtggaacaga aggggataac tggcttatca agggtgtggc ctttgaaaaa gctcagtttg
     7381 gcaagcacat cattgtttca gccattgaac atccagcagt caaagagtca gccctctggt
     7441 tgaaaagtca aggatttgaa gtggattttg ctccagttga taagaaaggc ttggtcgatg
     7501 ttgaggcgtt agcaggtttg atacggcatg atacaatcct cgtttccatc atggctgtga
     7561 acaatgaaat cggctctatc caacctattg aggctatttc agaattcttg gcagacaagc
     7621 cgactatttc cttccacgtt gatgcggttc aggcgcttgc caaaattccg actgaaaagt
     7681 atctgacaga acgggtggat tgcgcgactt tctctagtca caagttccac ggggttcgag
     7741 gtgttggctt tgtctatatc aaatctggca agaagattac acctcttctt acaggtggtg
     7801 gccaggagcg agattatcgt tcgacaactg aaaatgtggc agggattgca gcgacagcca
     7861 aggccctccg tttgtctatg gaaaagctag atatctttag gagcaagact gggcagatga
     7921 aggcagtgat tcgccaagct cttctgaact atccggatat ttttgtcttt tcagatgagg
     7981 aaaactttgc acctcatatt ctgacttttg gaatcaaagg tgttcgaggt gaagtcatcg
     8041 ttcacgcctt tgaagactat gatattttca tctcaacaac ctcagcttgt tcatctaagg
     8101 caggaaaacc agccggtacc ttgattgcca tgggagtgga caaagataag gccaagtcag
     8161 ctgtgcgtct tagcctagac ttggaaaatg atatgagtca ggtcgagcag tttttgacca
     8221 agttaaaatt gatttacaat caaactagaa aagtaagata ggagcattca tgcagtattc
     8281 agaaattatg attcgctacg gagagttgtc aaccaagggt aaaaaccgta tgcgtttcat
     8341 caataaactt cgtaataata tttcggacgt tttgtctatc tatacccaag ttaaggtaac
     8401 agcagatcgc gaccgtgccc acgcttacct caatggagct gattacacag cagttgcaga
     8461 atctctcaaa caagtttttg gaattcaaaa cttttctcct gtttataagg ttgaaaaatc
     8521 tgtagaagtt ttgaagtctt ctgtccaaga gattatgcgg gacatctaca aggaaggtat
     8581 gacctttaag atttctagca agcgtagcga ccacaacttt gaacttgata gtcgtgaact
     8641 caaccaaaca cttggagggg ctgtattcga agccattcca aatgtgcaag ttcaaatgaa
     8701 aagtcctgac atcaatcttc aggtggagat tcgtgaagaa gcagcctatc tttcttatga
     8761 aaccattcgt ggggctggtg gtttgccagt tggaacttca ggtaaaggga tgctcatgtt
     8821 gtcaggaggg attgactcac ctgtagcagg ttatcttgct cttaagcgtg gggtggatat
     8881 cgaggcagtt cactttgcta gtccaccata tactagtcct ggtgccctca agaaagcgca
     8941 ggacttgacc cgtaaattga ccaagtttgg cggaaatatc cagtttatag aggtgccttt
     9001 cacagagatt caagaggaaa tcaaagccaa agcgccagaa gcttatttga tgactctaac
     9061 tcgtcgcttt atgatgcgga ttactgaccg tattcgtgag gtacgaaatg gtttggttat
     9121 catcaatggg gaaagtctag gtcaagtagc cagccaaacc cttgaaagta tgaaggctat
     9181 caatgctgtt accaacactc ccatcattcg tcctgtggtt accatggaca agttggaaat
     9241 cattgacatc gcccaggaaa tcgatacctt tgacatttca atccaaccgt ttgaagactg
     9301 ttgtaccatt tttgcaccag atcgtccaaa aacaaatcct aaaattaaga atgcggagca
     9361 gtacgaagcg cgtatggatg ttgaaggctt ggttgagcga gcagtggctg gaatcatgat
     9421 tactgaaatc acacctcaag ccgaaaaaga tgaagttgat gacttgattg acaatctgct
     9481 ctaattcaga aaatccaaaa gaatagcgaa aatcagtaaa aaaagttagt tttttctcta
     9541 aaaacaggta aaaaactaac tttttttatt tttatgatat aatgatataa aattttgaat
     9601 atagagagtt ttctgacaat gaatcaatcc tacttttatc taaaaatgaa agaacacaaa
     9661 ctcaaggttc cttatacagg taaggagcgc cgtgtacgta ttcttcttcc taaagattat
     9721 gagaaagata cagaccgttc ctatcctgtt gtatactttc atgacgggca aaatgttttt
     9781 aatagcaaag agtctttcat tggacattca tggaagatta tcccagctat caaacgaaat
     9841 ccggatatca gtcgcatgat tgtcgttgct attgacaatg atggtatggg gcggatgaat
     9901 gagtatgcgg cttggaagtt ccaagaatct cctatcccag ggcagcagtt tggtggtaag
     9961 ggtgtggagt atgctgagtt tgtcatggag gtggtcaagc cttttatcga tgagacctat
    10021 cgtacaaaag cagactgcca gcatacggct atgattggtt cctcactagg aggcaatatt
    10081 acccagttta tcggtttgga ataccaagac caaattggtt gcttgggcgt tttttcatct
    10141 gcaaactggc tccaccaaga agcctttaac cgctatttcg agtgccagaa actatcgcct
    10201 gaccagcgca tcttcatcta tgtaggaaca gaagaagcag atgatacaga caagaccttg
    10261 atggatggca atatcaaaca agcctatatc gactcgtcgc tttgctatta ccatgatttg
    10321 atagcagggg gagtacatct ggataatctt gtgctaaaag ttcagtctgg tgccatccat
    10381 agtgaaatcc cttggtcaga aaatctacca gattgtctga gattttttgc agaaaaatgg
    10441 taagttaaga aaggaaaaaa cgaaatgcat attgaacatc ttagccactg gagtggtcat
    10501 cttaaccgtg aaatgtacct taaccgttat ggacatggtg ggattccagt tgtggtcttt
    10561 gcttcatcag gtggtagtca caacgaatac tatgattttg gcatgattga tgcctgtgct
    10621 tcctttatcg aggaaggcct tgtccagttc tttaccctat ctagtttgga tagtgagagc
    10681 tggttggcta cttggaaaaa tgctcatgac caagcggaaa tgcaccgtgc ctacgaacgt
    10741 tatgtgattg aggaggccat tcttttatca agcacaagac aggttggttt gatggcatga
    10801 tgacgacagg ttgctctatg ggagcctatc atgcactcaa tttcttcctc cagcatccag
    10861 atgtctttac caaagtgatt gctctcagtg gtgtttacga cgcacgtttc tttgtcggtg
    10921 attactacaa cgatgatgct atttaccaaa actcgccagt agattatatt tggaaccaaa
    10981 acgacggctg gtttattgac cgttaccgtc aggcagagat tgtgctgtgt acggggcttg
    11041 gagcctggga acaagatggt ttgccatcct tttacaagct caaagaagcc tttgacaaga
    11101 aacaaattcc agcctggttt gctgaatggg gacatgatgt cgcccatgac tgggaatggt
    11161 ggcgtaaaca aatgccttat ttcctcggta atctctattt ataaaaggag ttacctatga
    11221 attaccttgt tatttctccc tactatccac aaaactttca acagtttacc atcgaactag
    11281 ctaataaagg catcacagtc ttgggaattg gtcaagagtc ttacgagcaa ttggatgagc
    11341 ccttgcgcaa tagcttgacc gagtattttc gtgttgataa tcttgagaac atagatgaag
    11401 tcaaacgtgc agttgctttt ctcttttata aacatggtcc aattggccgc atcgagtctc
    11461 acaatgaata ctggcttgag ctagacgcaa cactcagaga acaattcaat gtttttggtg
    11521 ccaaaccaga ggatctcaaa aagacgaaat ataagtctga aatgaagaaa cttttcaaaa
    11581 aagcaggtgt tcctgtggta cctggagctg ttatcaagac ggaagcagat gttgatcaag
    11641 cagtgaaaga aatcggtctt ccaatgattg ccaaacctga taatggagtg ggagcagccg
    11701 caacctttaa acttgagaca gaagacgata tcaatcactt caagcaagaa tgggaccatt
    11761 caacccttta tttctttgaa aaatttgtca cttccagcga aatctgtacc tttgacgggc
    11821 tcgtggacaa ggatggaaag attgtcttct caacaacctt tgactacgcc tatacaccgc
    11881 ttgacctcat gatttataag atggacaatt cttattatgt gctcaaggat atggatccta
    11941 aactgcgcaa gtatggggaa gcaattgtca aagaatttgg tatgaaagaa cggtttttcc
    12001 atattgagtt cttccgtgag ggggacgatt atattaccat cgagtacaat aaccgccctg
    12061 caggtggttt taccattgat gtttataact ttgctcattc cttggacctt tatcgtggct
    12121 atgcagctat tgtcgcagga gaggagttcc cggcgtcaga ctttgaaact cagtattgtt
    12181 tggctacttc tcgccgtgca aatgctcact atgtttattc agaagaggat ttgcttgcca
    12241 aatatagcca gcagttcaag gttaaaaaag tcatgccagc tgccttcgcg gaacttcaag
    12301 gagattacct gtatatgctg accactccga gtcgacaaga aatggagcag atgattgcag
    12361 atttcggaca acgtcaagaa taagaactat cggattaagg aaattaactc ccttaatcct
    12421 tttgttttgt ctgataaaaa ataagagcat cccaacaagg tagctatcat aaaacttgtt
    12481 cgataactat ttgaagcagg attaggtggt cagaaattaa attttaatat ttcaattgag
    12541 tcatagtatt gtgtttgcgt atccttaaat cagctaaaag gatccatgac gacacctata
    12601 cgatatagtt ttcaagatac caaacaagtc tattaatatt caatgaaaat caaagagcaa
    12661 actaggaagc tagccgcagg tttctcaaaa cactgttttg aggttgtgga tagaactgac
    12721 agagtcagta tcatatacct acggcaaggt gaagctgacg tggtttgaag agattttcga
    12781 agagtataaa atattcaggt gacgcataga tatagttaat tgaagctttg tttgaaatct
    12841 gataaaataa tgatattact aagttttaaa aactaaagaa aagggaagat atgattacag
    12901 gcgaattaaa aaataaaatc gatcagctgt gggaaattct ttggacagaa ggaaacgcaa
    12961 atcctttaac aaatattgaa cagttgactt atctcttatt tatgaaagat ttggatagtg
    13021 tcgagcttgg acgtgaaagt gatgctgaat ttctagggat tccttatgag ggagtttttc
    13081 caaaagataa acctgaatac cgttggtcaa cttttaaaaa tataggagat gctcaggaag
    13141 tttatcgttt aatgactcag gagatttttc cgtttattaa aaatctcaag ggggatacag
    13201 atgatacagc cttttcacga tatatgcgag aagctatttt tcaaataaat aaacctgcta
    13261 cgcttcaaaa ggcaatttct atcttagatg tttttccaac taggggatta gatgtagatt
    13321 ttgataatga caaacaaagt attactgata tcggagatat ctatgaatat ctgttatcaa
    13381 aattgtcgac cgcaggtaaa aatggacagt tccgtacacc tcgtcacatc atcgatatga
    13441 tggttgagtt gatgcaaccg actatcaaag atatcatctc agatcccgct atgggttctg
    13501 ctggcttctt agtatctgct agccgttact taaagcgtaa gaaagatgaa tgggaaacca
    13561 atacagataa tatcaatcat tttcataatc agatgtttca tggaaatgat acggatacga
    13621 ctatgttgag acttggggcg atgaacatga tgctacatgg agtagaaaat ccacaaatca
    13681 gttaccttga ctcgctgtct caagataatg aagaagccga taaatatact ttggttttag
    13741 caaatcctcc ttttaagggc tcacttgact acaattcaac ctctaatgac cttcttgcaa
    13801 ccgtaaaaac caaaaaaaca gaattactct ttctttctct tttcttgcga actttaaaac
    13861 caggtggacg agcagcagtt atcgtacctg atggtgtcct ttttggttcg tctaaagctc
    13921 ataaaggaat tcgtcaggaa attgtagaga atcataagct tgatgctgta atctcaatgc
    13981 ctagtggtgt gttcaagcct tatgctggag tttcaactgc cattctcatc tttacaaaaa
    14041 ctggtaatgg tggtactgac aaagtctggt tttacgatat gaaagcggat ggtttaagtt
    14101 tggatgataa gcgacaaccg attagcgaca atgatattcc agatattatc gaacgctttc
    14161 atcatcttga aaaagaagca gaacgtcaga gaacggatca atctttcttt gttccagttg
    14221 ctgagataaa ggaaaatgat tatgatttgt ctatcaataa atataaagag attgagtatg
    14281 aaaaagttga gtatgaacca acagaggtca tcttaaagaa aatcaatgat ttagaaaaag
    14341 aaattcaagc tggcttggct gaattagaaa aattactcaa gtagggaggt ggctgtatga
    14401 aaaaagtgaa gttgggggaa gtcttatctc taaaaaaagg caagaaagcc actgtacttg
    14461 ctgaacaaac aactctaagc caacgttata ttcaaataga tgatttaaga aataataata
    14521 atttaaaatt cactgaaagt ttaaatatga ctgaagcact cccagatgat attctgatag
    14581 catgggatgg agctaatgca ggaacagttg gttatggatt atcgggagct gttggtagta
    14641 caattacggt cttaaaaaag aatgagcgat acaaagaaaa aattatatca gattacttgg
    14701 gagtcttttt ggaaagtaaa tcgcagtatt tacgagaaca ttcaacaggt gcaacaattc
    14761 ctcatttaaa caagaatata ttacttgatt tacaattaga attgctaggt atcgaagaac
    14821 aagagaacat tatctgtatt cttaatacga ttaaagggct tattactaaa agaaaattgc
    14881 agttagatga acta
//
LOCUS       DAVHEW010000045        14665 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVHEW010000045 DAVHEW010000000
VERSION     DAVHEW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.045"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..422
                     /locus_tag="VVL53_001264"
     CDS             <1..422
                     /locus_tag="VVL53_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4183438.1"
                     /translation="SDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHF
                     QNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEKIVNALQLHYSNAKLEATNNLIK
                     LIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(472..1182)
                     /gene="deoD"
                     /locus_tag="VVL53_001265"
     CDS             complement(472..1182)
                     /gene="deoD"
                     /locus_tag="VVL53_001265"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU4183439.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1836..2348)
                     /locus_tag="VVL53_001266"
                     /pseudo
     CDS             complement(1836..2348)
                     /locus_tag="VVL53_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
     gene            complement(2373..3353)
                     /locus_tag="VVL53_001267"
                     /pseudo
     CDS             complement(2373..3353)
                     /locus_tag="VVL53_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(3829..4638)
                     /locus_tag="VVL53_001268"
     CDS             complement(3829..4638)
                     /locus_tag="VVL53_001268"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU4183440.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(4654..5196)
                     /locus_tag="VVL53_001269"
     CDS             complement(4654..5196)
                     /locus_tag="VVL53_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU4183441.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQIFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVENLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            complement(5198..6409)
                     /locus_tag="VVL53_001270"
     CDS             complement(5198..6409)
                     /locus_tag="VVL53_001270"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU4183442.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLDVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(6423..7106)
                     /gene="rpiA"
                     /locus_tag="VVL53_001271"
     CDS             complement(6423..7106)
                     /gene="rpiA"
                     /locus_tag="VVL53_001271"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU4183443.1"
                     /translation="MENLKKMAGITAAEFIKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTSKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(7404..8243)
                     /locus_tag="VVL53_001272"
     CDS             complement(7404..8243)
                     /locus_tag="VVL53_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU4183444.1"
                     /translation="MKVIDQTLLEKVIIERSCTSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLQEQLEKAEVVLLGPGL
                     RDDTFGENLVKQVFASLKKNQILIVDGGALTILARTSLLFPSNQLILTPHQKEWEKLS
                     GIAIEKQNEGTTSSALTSFPQGTILVEKGPATRIWQVGQSDYYQLKVGGPYQATGGMG
                     DTLAGMIAGFAGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(8258..9079)
                     /locus_tag="VVL53_001273"
     CDS             complement(8258..9079)
                     /locus_tag="VVL53_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEU4183445.1"
                     /translation="MILKIYNGEYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGRLLSAVKAFGRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVPGYIFDGYHAVKIKDMLNLLS
                     ELYICIIPTHNKSQFENIIPTKIQDRVYYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(9217..10074)
                     /locus_tag="VVL53_001274"
     CDS             complement(9217..10074)
                     /locus_tag="VVL53_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU4183446.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(10253..10987)
                     /locus_tag="VVL53_001275"
     CDS             complement(10253..10987)
                     /locus_tag="VVL53_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4183447.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGDSKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQGQRTKDFLSKVL"
     gene            complement(10987..11670)
                     /locus_tag="VVL53_001276"
     CDS             complement(10987..11670)
                     /locus_tag="VVL53_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4183448.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            11860..12090
                     /locus_tag="VVL53_001277"
     CDS             11860..12090
                     /locus_tag="VVL53_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU4183449.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            12319..14577
                     /locus_tag="VVL53_001278"
     CDS             12319..14577
                     /locus_tag="VVL53_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU4183450.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRSSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPSISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         4411 a         3286 c         2637 g         4331 t
ORIGIN      
        1 tgagcgataa acgtttttat cgccctactt ttcgcatgca cttaacaaat aaagaaattc
       61 ttgacaagat tttaagctat tcagaagact tgaaacacca ctatcagatc tatcaactct
      121 tactttttca ctttcagaac aaagaccctg agaaattttt cggactcatt gaggacaatc
      181 tgaagcaggt tcatcctctt tttcagactg tctttaaaac ctttctaaag aacaaagaga
      241 aaatcgtcaa cgcccttcaa ctacactatt ctaatgccaa actggaagcg accaataatc
      301 tcatcaaact tatcaagcgc aatgcctttg gttttcggaa ctttgaaaac ttcaaaaaac
      361 ggatttttat cgccctgaat atcaaaaaag aaaggacgaa atttgtcctt tctcaagctt
      421 agcttttttt caacccacta cagttgacaa agagcccctt tttggctata attattctgc
      481 aatcaaggtt tccaaaccaa ccttcatcat atcagtgaag gtattttgac gttcttctgc
      541 agttgtgtct tcgtctggat tgaccaagct atcagagatg gtcatgatag ctagcgcatc
      601 aacatggtat tgggcagcaa gatagtaaag agctgctgct tccatttcca cagccttgac
      661 tccccattta ccaagctcga tattcttttc aaagtaattt gagtaaaaga catcagatga
      721 caaaacgttc ccaacgtgag tagtcatacc aagttttttg gcgatatggt aggctttatc
      781 aagcaaatca aagctagcaa tttgtggaaa atcgtactgt ggccagtcat tacgaacgat
      841 gtttgagttg gttgcagccg cctgcgccaa aactaattca cgaacatgaa cctcttcatt
      901 caaagaacct gcagttccca cacgaatcaa tttcttcaca ccgtagtcta cgattaactc
      961 acgcgcataa atcgaaatag atggcattcc catcccagtt cccatgacag atacacagtg
     1021 acccttgtaa gtaccagtgt aaccaaacat gttacgcact tcgttaaaac aaacagcatc
     1081 atcaaggaaa ttctccgcaa taaacttagc acgaagagga tccccaggaa gaagaatttt
     1141 atcagcaatt tcaccctgct gagcagcaat atggatagac ataatttatg atacaaagag
     1201 cgagaagaaa acgactgaaa attaggaacc tgacgagaaa tcctgatttt tcagtcagat
     1261 tatctatttt ccgagttttc cgctcgtgtt caaatcaaaa cacacgctct acctttcttt
     1321 attttatatt ttatattgag aaagatacca aacccatcaa aaagcgaagg gaaaatagga
     1381 gttgggcgca gtgagcgatg ctcgctagat caactatctt tttcccacta cttttagggt
     1441 ggggtcaatt cctttcttaa ttttgattta gaggagagtc gcccgtattc agttcagcga
     1501 atacagttta cccatccttt cgtttttgtt tttagaaaag ttttctactc gtgttcaaat
     1561 tagaacacgc gctctacctt tctgtttata ctcttcgaaa atctcttcaa accacgtcaa
     1621 cgtcgccttg gattatatat gtgactgact tcgtcagtct tatctacaac ctcaaagcag
     1681 tgctttgagc aacctgcggc tagtttctta gtttgctctt tgattttcat tgagtattaa
     1741 ttttgttctt tcatagagag ctacctgagt cctattcaag ctcaggtagt actttcttat
     1801 aaactagaca aactaactgt cattctacca tcagattaca agacatcatc gtcactcacc
     1861 ttggaattca atgtcgtacc ccaatgggta attttacggt ggggttgagc taaaattggt
     1921 ctgttttcat agattgtttg ccatctattc catagtaggc ccgtcttttt ctcaatctta
     1981 actcgcagat ttctcatatt ttctttgatt gggaggttga ggacaaaacc tgcagtctgg
     2041 ttgcgaccgc ttccttccca agaatgacta cgaacaactt ggtttccatc tttatctact
     2101 ggaacttctt cccaagttat ggagtagcgg gcaatgtaag ctccactgtg ttgaattgtc
     2161 aatgttttat ccttcacagg agtctgactg attggttgaa ctggcttaga aacttgtgtc
     2221 gccgtttcag cattcgtagc tataaagcag aaaacttcaa cttctgcaag actgagggca
     2281 ttatagcctt ctagctaaat ccgaacatag cgcgcttttt tatggttaat cgtaatttgt
     2341 gctgacacat ctttaggaga tgttatacgt tttcactcaa tttctttacc agaactgtct
     2401 aaaagaatga catcaaagtt tgccaatcta tcctgggcag tgtctgttcg gttgtaaata
     2461 ttgatttggc gaatggtttc ttctttagcc aaatctactt gccaccaagg cttagattgg
     2521 aagtttgtat gagtgacaga attgtgacca tagttaccat cgactttgcc atccacagct
     2581 cttctagcat ctcctcagaa agctgtcgaa ctttgagtaa cctgttttcc tgaggcaata
     2641 ttttccattg gttcagcgct tggttgacta gaagatcgag gcagagcctg tttcttaaag
     2701 aatcgcactt tatccaattt cggatcacga taaccatgtt tatccaaggt agcttgttta
     2761 ccaatattaa agttaggaat atcattcatt ggactttcaa aatagccacg agaacggtgt
     2821 ttaaagcgtg aattacctgt aaatgtaaca atatcacgat tattatgaat caagggagtc
     2881 acaacattgt cattatcaac cacataatgc ttaatttttc cactaacagc taatttacga
     2941 ctttccaaac caacatccca gaaaccattc ttagcattcc aaacagttct ggaagtaatc
     3001 actttaggag cactgaaagt tgtcactttc ctcaatacat ggttataaaa atcaggatat
     3061 ttttggtaag cttcaaccgc tgcaatctga gctaggtaac atccaagaga atgacctgtc
     3121 atatacaact tagtaatact tatatccttg gctaattccc caacaacctt gcggatatca
     3181 tccgcttgag ctggttatgt tatctctact tttgactctt cccttttgtt ttcaagaact
     3241 tcacttgctt ccggaagcgt ttgaggagct agtcttggct cctctctaag aggagtttct
     3301 agttttggag catcattttc tgactctttc aattcctcct tagaatctag cacacctatt
     3361 accacttcag atgttacatc ttgctttact tcattggctg aaattccagc tggagctaga
     3421 aatcaaagcc gactacaaca gatacaactc cgatacttaa ttttttatgc caaagcgcat
     3481 aattcgttga ccaattttca ttatttctta tctttctttt cgtttattta caagcagact
     3541 caagcgaaat ctcattgtgc tatatatgat aagactgaga aattcagaaa gtcaattcat
     3601 tctcatctct ccttatcata ctagtctgct tggctaggaa gctaatgcta gtatcctaga
     3661 atcttgtctt tcacaatgct ggctatcaga tcagacatac ctattcttcc gcttctttca
     3721 gttataagag caggaagaag ctcatagtat tcctctgttt cttctttaat ccggataggc
     3781 agtcaatcct gaaaaaacag aggcactccc ccttttaaat cttttttctt acaattcagc
     3841 aagaatcgct ttaagcaagc ctttgaaatc acctttaaca cgttcagtca cttctacaac
     3901 ttcttcgtga ttgagttctt cttggaaacc agccgcaaag ttagtgatac atgaaattcc
     3961 cagaactttc aagccagagt gggctgccac gataacttca ggaaccgtag acataccaac
     4021 tgcatctgct cccagtgtct tataggaacg aatttctgct ggtgtttcat aagtcggacc
     4081 agtaactccg atatagacac cttcatcaag cttgatatta agttttttag ccacttcatg
     4141 ggcagtggca cggtattctg gtgtgtaggc cctagacata tctgggaaac gtgggccaaa
     4201 gtcatccaag ttttcaccca tcaatggatt ttgccccgtc atgttgatat ggtctgagat
     4261 agccatcaag gtaccaggac caaatccgat accgccagct gcattggtta caataacacc
     4321 ttcacatcca agaactttca tcacacgaac tgggaaagtc accacttcca gaggattccc
     4381 ttcatagaaa tggaaacgac cttgaagagc caagaccttg cgacctgcca gttcaccata
     4441 taccaattta ccagcatgac cgactactgt tgaacggccc cagtttggaa tctcagcata
     4501 gtctactaca actggatttt cgatttcttc tgccaattct ccaagtcctg acccaaggat
     4561 tagaccgaac tcaggggctg caattccctt ttctttcagg aaagtagcag tttcatggat
     4621 tttgtttaaa aatgtcattg tgtgtctcct ttattatttt tttagcattt gaccaatttt
     4681 gtcaaaggtt ttagcattac gaatagtaat gtggcggtag aaaggcacct tcagcaagta
     4741 cttatgatag gcagtcttag aataggattc ttcagaaaat ttcccccaga aaatcccaag
     4801 ttttccaaaa taaagcactt catctttcag ctctaaattt tcaactgtcg cgatgacttg
     4861 gtccacatcc aaaccctcag tgtaaaagag aaaatctttt cgtgccaagt ctctgctcca
     4921 ccaagctggt agattttcaa gttccgcctc aaagtcctct agactcagta aagaaaagat
     4981 ctgaataaat ggataatgga ctgcaaagaa agtctctagc ttttcaacca attgggcttt
     5041 ggaatctatc gaagtaaaga aaatattgcc actgttgatg tagctctcaa ccttttccag
     5101 tcctaagttt gtcaattctt gacgaagctc cgccatgacg accttattct taccaccaac
     5161 attgatacct ctcaccagca aagcatagcg cgtcatctta taccaattta tctaagaaac
     5221 tttccccaat catagcagtt tccacaccaa agttatcggc aacagtcgct gaaatatctg
     5281 caaaatgtcc tactggaatg agaccatttc ctttaaaggc agggctatag gccaacaatg
     5341 gaatatattc ccgagtgtga tccgttcctg catacgttgg gtcatttcca tggtccgcag
     5401 taatcaagag aaggtcattc tctctcatag ctgcgataat ttcaggtaag cgttcatcaa
     5461 actcatgcaa gcaatcacgg taaccgtgag cattacgacg atggccgtaa agggcatcaa
     5521 agtcaactag gtttgtgaat gagaatcctt tttcaaactc agcaagtccc atagtcttca
     5581 atagtgtatc aattccatga ctatttgact tgttgtgacc catgtcatgg ttgataccag
     5641 caccgttaaa gatatcgttg attttaccca cagcataagt atcgatacca gcctcattca
     5701 atttatccaa aacagttggg gaaaatggag atacagccaa gtcacgacgg tttgccgtac
     5761 gagtgaagtt acctggttca cctacataag ggcgagcaat gatgcgacca agaagggcag
     5821 gacgctcaag ggtaatcgaa cgagcgtatt cacagatacg gtacaattca tccaaaggaa
     5881 taatgtcttc gtgggcagca atctgcaaaa cagggtcagc tgaagtatag ataatcaact
     5941 ctccagtttc catctgacgt ggtccaaaat catcgataac agccgttcct gaataaggtt
     6001 tgttggcttc acgaataacc ttgcgtcctg agaattcttc gatttttgtc aggatttctt
     6061 ctgggaatcc gttccagaaa gtatcgaaag gctcagtaat gttgagtccc atgatttccc
     6121 agtgtccagt catagtatcc ttaccaagag atacttcctc taattttgtt gcatatccag
     6181 ttggattgct ttcagctgct acagtcttaa gaggagtttc acgaggaata tttccaagac
     6241 ctattttagc catgtttggg acatccaaac caactgtttt tgaaatgtgt cccagtgtgt
     6301 cagaagctcc atctggaacc cctgcattga caaagttatt agcatctggt gctgcaccga
     6361 ttcctacaga atccagtacc accaaatgaa tacgattaaa tttagacatc ttatgccccc
     6421 ttctattttc ctttttttga agttgaaatc tgaactccat ctcgtccagc aacgattacc
     6481 ttatccacca tttggttgaa taaaccatgc tccacaacac caacgacatg gtccaattct
     6541 tgtccaaaag caattggatt ttcaatgaca tccaaggcga ggtcaatgat aaaattctgc
     6601 atatccgtca caaaacgttg gccgtctttt tcacggaaac ttggtttgta gccagctcgt
     6661 tcaaaatgac gaaagacctg ctctgcacca tactgaacca cttctactgg caatttaaaa
     6721 gcacctagtt tttcgaccag cttgctttca tccaccaccc aaatgtattc ttttgatggt
     6781 gttgcgacca ccttttccat gagaagggca ccaccaccgc ctttgattcc attaaactga
     6841 ctatccactt catccgcccc gtcgactgtc acatcgacaa agtctacttg gtcaatagac
     6901 ttgagcggga tattgagccc ttcagcctgt ttactggtca cactagaagt cgtcacagct
     6961 gtaatctgca agccttcttc cttgattcga cgaccgattt cttcgacaaa ataataggca
     7021 gtagaacctg ttcctagccc tacaaccatc ccatccttga taaattcagc agccgtgata
     7081 cctgccattt tcttcagatt ttccactcaa acacctccat taaagagcta cctctattat
     7141 accatgtaaa ggcttttatt tcatctaaaa gtacctttag aatactgctt atttatcctt
     7201 gaaattgaat ttttttaata aaagtagagc tctaacaaat caatttaagt acggatgtaa
     7261 accaaatcaa ctctaacaaa aatagattta caagagtaaa aaaacgaaca aatttagttt
     7321 cttagcaaga agaatactaa acgttcgttt tttgtgaatt aagaataatg atacaaatta
     7381 tcaacatttt ttgtctaact attttagaca tatcttttca ttactttagg aagacaatta
     7441 ctaatttccg tcggcaagac cacataattt tcttgagata gttcttgggc tatggctgaa
     7501 tgaagatggg ttgctactgc cacacgttcg tagagactgg cctgtcgaaa ttggcctgca
     7561 aatcctgcaa tcattccagc cagtgtatca cccataccac cagtcgcctg atagggaccg
     7621 ccaaccttta actggtaata atcagactgg ccaacttgcc aaatacgagt agctggacct
     7681 ttctctacca aaattgttcc ttgagggaaa gaagtcaggg cactagatgt tgtaccttcg
     7741 ttttgctttt caatagcaat accagacagt ttttcccatt ctttttggtg gggagttaag
     7801 ataagctggt tagatggaaa caacaaactt gtcctagcaa ggatggttaa ggcccctcca
     7861 tctacaatca aaatctgatt cttttttaag ctagcaaaga cctgttttac aagattttct
     7921 ccaaacgtat cgtctcgtaa accaggcccc agcaagacaa cttctgcctt ctccaattgc
     7981 tcttgtaaca attgctgatc ttgcagagaa aaggccatag cctcaggcaa atggctgtgt
     8041 agagcaggga tattttccct gtccgttcca acggttacca atcctgcacc gctttttaca
     8101 gctgctaaag cagccatgat gatggcacca ccataaggat aagtcccacc aagcaacagc
     8161 agacgaccgt agtctccttt atgacttgta caagaacgtt caataatgac tttttctagt
     8221 aaggtttgat caatcacttt catcgttttt tcctcaacta tcctttatct gattgtttta
     8281 aaacaacttg atagactttc ttagtccact cttctaagtc ttctccagca tagtcaagat
     8341 aatacaccct atcttgtatt ttggttggaa taatattttc aaattggctc ttattatgag
     8401 ttggaataat gcaaatatac aactcactta ataaattaag catgtcctta atttttacag
     8461 catggtaacc atcaaaaata taaccaggaa ctttaatcaa atctgtatag ataaaatgaa
     8521 aacttactcc aggaataaac ttgtcttgta attctttctc tgaaggagct cgacctaata
     8581 aacgctccat cgccaatcga taaccagaac ttgtatttga ccaccctaac atgacatagt
     8641 caaaataatc tatcggatca gatgcagcat ttcgactatc caaaaccaac tcctcagcat
     8701 ctcgcccaaa ggctttcaca gcagataaaa gacgaccagt tttaaaaata gaaatcgctg
     8761 catctatagt cgtagtatga ctacagtagt ctgaagtcac taacctctta tgtaggtcat
     8821 aagtcgaggc tactctttta tctgtaggag taaatggtgg cagaaaggga tgctgacaga
     8881 tgtaatctaa aatttctttt tttaaacaag aatcagcaca ctcaatactt gtttgacgtt
     8941 tatgaagttt ttcgtaagct ataaatttag caattttttc taattcccac tcttgaatat
     9001 ttggataatc aattagtgct aagtagtata ttccatccca ttgtaaacta tattccccat
     9061 tataaatttt taaaatcatc taatcacctc actccattat acaacaaaaa ggaggcgcaa
     9121 acctcctttt ataaacttat ttctaaaatt taatattcat ttctatcatt ttagatatag
     9181 ctatagaaaa tacactttcc tcagaaaatt tttatcttat tttctatcca atgtcctaag
     9241 tgctgcctga taggtttgct ccatcagcat agtaatggtc ataggaccga cacctccagg
     9301 gactggcgta atgtggctag caagtggggc aaccgcctca taatcaacat ccccacagag
     9361 cttaccattt tcatcgcggt tcatcccaac gtcaatgact accgcacctg gtttgacaaa
     9421 gtcagcagtc acaaacttgg cacgaccgat tgcaacaacc agaatatctg cttttgcagc
     9481 caccttggaa agattatgag tacgtgagtg agtcaaggtt actgttgcat tctttgccaa
     9541 aagaagctgg gccataggtt ttccgacaat attggatcga ccgatgacga ctgcattttt
     9601 accttccaag tcaatcccat attcatggaa catttccata attcctgccg gtgtcgaagg
     9661 aatcatgact ggatgaccag accaaagacg ccccatgttt agaggatgga aaccatccac
     9721 atccttttct gggtcaatag ccaatagaac cgcctcttca tcaatgtgtt ttggtaatgg
     9781 caactgaacc aaaatcccat gccaagctgg atcctgattg tatttagcaa tcaggtctaa
     9841 caattcctct tgagtaatgg tctctggaac ccgtactact tcgctacgga aaccagccgc
     9901 aagggctgac ctctccttgt tgcgaacgta gacttggctg gctggattgt ccccaaccaa
     9961 aatcactacc aaaccaggca ctagacctgt ttcttccttt aattttgcag tcttttcagc
    10021 caactgcccc tgcaatttgg ccgctaaagc tttcccatca ataatctgtg tcatatcact
    10081 tctcttttct tacaaatatc tttaattata ccaaaaaata caatgtagct ctaacacttc
    10141 tttcctaggc aatcctatag ctttaaacta taagaaaaag ctctggttga gcttttcact
    10201 aatcttctct ttaacgttta aagctggcta caaatagcta aacaaagcta acttataaaa
    10261 ccttactcaa gaagtcttta gtcctttgtc cttgggtttg ttcaaaaatc tgctcaggtg
    10321 ttccgtcttc aacaaccaca ccgtctgcca taaagatgac acgatctgcc acctcacggg
    10381 caaatcccat ctcatgtgtt acgataacca tggtcattcc tgacttggct agatcttgca
    10441 taacagccag aacttctcca accatctcag gatctagggc tgaagttggc tcgtcaaaga
    10501 gcaaaacatc tggttccata gccaacccac gcgcgatggc aatccgctgt tgctggccac
    10561 ctgacaaact ctgtggataa gcgtctgcct tatctggcaa accaactttt tccaaaagtt
    10621 cctgagctct tttctctgca acggccttac tgtcaccttt ggtcttgata ggggacaagg
    10681 tgatattttc catcacagtc atattaggaa agagattgaa ttgttgaaaa accatgccca
    10741 tcttctcacg catggcaaac aggtcattct tcttgtccgt aatatcgact ccctcaaaga
    10801 taaccttccc cttggttgct tcttccaaca aattcataga gcgaagcaag gtagatttcc
    10861 cgctccctga aggaccgatg ataacgacaa cttctcctct tttaatctcg aggttgatgc
    10921 ccttcaatac ttcattcttt ccaaaggatt tatgtaaatt ttcaattttt atcaaggttt
    10981 ctgtcattat ttcttatctc cttgtcccat atgtttttca aaagctttca aggctactgt
    11041 cagaatagag gtcataatca agtagtaaaa tgctgcaaat aaaagtggtg ttaaaggtag
    11101 ataggttgtt gtagaaactg ttgtagcccc attccacaac tccatgaccc caatagctga
    11161 taagagggag ctgtccttga taatggtgat aaattcgttc cccaatgctg gcaagatatt
    11221 tttgactgct tgtggcaaaa tcacataacg catcgcattt ttaggacgaa tccctagcga
    11281 ataagccgct tctagctgac cttttggaac cgcattgatt ccggcacgaa cagtctccga
    11341 aacataagca ccactattca tagagataat caaaatccct ggaatcagac gcgaaaaatc
    11401 aacacctaaa attccaatct gaatagtcgg agcattgata tgcataagag caaaggcaat
    11461 cataatttga accatcatcg gtgtcccacg gaaaatccaa acgtacaagt tggccaacca
    11521 aacaagcggt ttaaactttg aacgttgccc aaaagccaag acaacaccca aaatagttcc
    11581 caaaaagata acacagatag aaataagaat cgtcacaaca gccccatagt taaaataagg
    11641 taaatactta ggtaaaaaag aaaaattcat agtcacactg ctttctaaaa tagaatactg
    11701 tatgattata acatgttttg aattaaaatt taaatttttt atccaattta tttaaaaata
    11761 tttaaactag cagaattagc gagaaatctt agttttttct agtcaagttg gcctccttta
    11821 tggtaaaata gtaacgataa gaaatgaagg agaatcaaaa tggaatcaca tttggttaga
    11881 atcatcaacc gccttgaagc aatggcaaac gatggcggaa atttaaaacg taattttgaa
    11941 cgcgaaggag ttgttgttgc agaagttgcg tacagccacg acgaagaaaa cggcccaatc
    12001 tttacccttc gtgacgtaga agatcgcgaa acgtatacgt ttgatagcat tgacttaatc
    12061 gcaatggaaa tttacgaact tctctactaa tctaaaacaa gctgggattg tccctgcatg
    12121 tctaagtaaa atcctttttg tctcacaaat aggatttttt tatagtccaa attcacaaaa
    12181 attttcattg taacaacttc tcaaagcttc aatcttttta cttgctttac accccaatcc
    12241 tgttataatg agagtataga atttgactat ttttgacctt taaacaagtc aggctaaaga
    12301 aagaaatgag gtagatgtat gctttgtcaa aactgtaaaa ttaacgactc aacaattcat
    12361 ctttacacca atctcaatgg aaaacaaaaa caaattgacc tctgtcaaaa ctgctataag
    12421 attatcaaaa cagatcctaa caatagcctc ttcaaaggta tgacggatct gaacaatcgt
    12481 gacttcgatc cctttggtga tttcttcaat gatctaaaca atttcagatc ttctagcaat
    12541 actcctccta ttcccccaac ccaatcaggt ggaggttacg gtggaaacgg cggttatggt
    12601 tcccaaaatc gtggatctgc tcaaactccg ccacctagcc aagaaaaagg cctgctggaa
    12661 gaatttggta ttaatgtaac tgaaattgcc cgtcgtggag acattgaccc cgttattggg
    12721 cgcgacgatg agattatccg tgtcatcgag attctcaatc gtagaaccaa gaataatcct
    12781 gtccttatcg gtgaacctgg tgtcggaaaa acggccgttg tcgaaggtct agctcagaaa
    12841 attgtcgatg gcgatgtgcc acataaactc caaggtaaac aagtcatccg tctggatgtg
    12901 gttagcttag ttcaaggaac ggggattcga ggacaatttg aagaacgcat gcaaaaactc
    12961 atggaagaaa ttcgcaaacg tgaagacatc atcctcttta tcgatgaaat ccatgaaatt
    13021 gttggtgctg gttctgcgag tgatggtaat atggacgcag gaaatatcct caagccagcc
    13081 cttgctcgtg gagaactgca actagtcggt gctactaccc tcaatgaata ccgtatcatt
    13141 gaaaaggatg ctgccctcga gcgtcgtatg cagcctgtta aagtcgatga accaacggtg
    13201 gacgaaacaa tcactattct caaagggatt caaaagaaat acgaagatta ccaccacgtt
    13261 caatatacag atgctgcgat tgaagcagct gcaactcttt ccaatcgcta catccaagat
    13321 cgcttcttgc ctgacaaggc cattgacctc ctagatgaag ctggttctaa gatgaacttg
    13381 accttgaatt ttgtggatcc taaagtaatt gatcagcgct tgattgaggc tgaaaatctc
    13441 aagtctcaag ctacacgaga agaagatttt gagaaggcgg cctacttccg cgaccagatt
    13501 gccaagtata aggaaatgca aaagaaaaag atcacagacc aggatactcc tagcatcagc
    13561 gagaaaacta ttgagcacat tatcgagcag aaaaccaata tccctgttgg tgatttgaaa
    13621 gagaaagaac aatctcaact catccatcta gccgaagatc tcaagtctca tgttattggt
    13681 caagatgatg cagtcgataa gattgccaag gctattcgcc gtaatcgtgt cggacttggt
    13741 acccctaacc gcccaatcgg aagcttcctc ttcgttgggc caactggtgt cggtaagaca
    13801 gaactttcca aacaactggc tatcgaactt tttggttctg ctgatagtat gattcgcttt
    13861 gatatgagtg aatacatgga aaaacatagt gtggctaagt tggtcggcgc tcctccaggt
    13921 tatgttggct atgatgaggc tggtcaatta actgaaaaag ttcgccacaa tccatattct
    13981 ctcatccttc tcgatgaagt ggaaaaagct cacccagatg ttatgcacat gtttcttcaa
    14041 gtcttggacg atggtcgttt gacagacggg caaggacgca ccgttagctt caaggatgcc
    14101 atcattatca tgacctcaaa tgcaggtaca ggaaagaccg aagctagcgt tggatttggt
    14161 gctgctagag aaggacgtac caattctgtc ctcggtgaac tcggtaactt ctttagccca
    14221 gagtttatga accgttttga tggcattatc gaatttaagg ctctcagcaa ggataacctc
    14281 cttcagattg tcgagctcat gctagcagat gttaacaagc gcctctctag caacaacatt
    14341 cgtttggatg taactgataa ggtcaaggaa aagttggttg acctaggtta tgatccaaaa
    14401 atgggagcac gcccacttcg tcggactatt caagactata ttgaggacac aatcactgac
    14461 tactaccttg aaaatccaag cgaaaaagat ctcaaagcag ttatgactag caagggaaac
    14521 attcagatta aatctgccaa aaaagctgaa gttaaaagtt ctgaaaaaga aaaataaatc
    14581 ctataaaaaa ggagtagaaa atgaaatttt tctgcttctt tttttactaa aataactgta
    14641 atttcttgac agcttgccct ttgtc
//
LOCUS       DAVHEW010000046        14623 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVHEW010000046 DAVHEW010000000
VERSION     DAVHEW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.046"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..300)
                     /locus_tag="VVL53_001279"
     CDS             complement(<1..300)
                     /locus_tag="VVL53_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 family protein"
                     /protein_id="HEU4183451.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAG"
     gene            392..1348
                     /locus_tag="VVL53_001280"
                     /pseudo
     CDS             392..1348
                     /locus_tag="VVL53_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            1332..1889
                     /locus_tag="VVL53_001281"
     CDS             1332..1889
                     /locus_tag="VVL53_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4183452.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            1882..2136
                     /locus_tag="VVL53_001282"
     CDS             1882..2136
                     /locus_tag="VVL53_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183453.1"
                     /translation="MDKQYLHEKLDVMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKDQKEIFESCCKKD"
     gene            2148..4202
                     /locus_tag="VVL53_001283"
     CDS             2148..4202
                     /locus_tag="VVL53_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4183454.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            4381..5241
                     /locus_tag="VVL53_001284"
     CDS             4381..5241
                     /locus_tag="VVL53_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4183455.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            5452..6162
                     /gene="ccdA2"
                     /locus_tag="VVL53_001285"
     CDS             5452..6162
                     /gene="ccdA2"
                     /locus_tag="VVL53_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU4183456.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            6182..6748
                     /locus_tag="VVL53_001286"
     CDS             6182..6748
                     /locus_tag="VVL53_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU4183457.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            6759..7871
                     /gene="msrB"
                     /locus_tag="VVL53_001287"
     CDS             6759..7871
                     /gene="msrB"
                     /locus_tag="VVL53_001287"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4183458.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQSISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            8229..8966
                     /locus_tag="VVL53_001288"
     CDS             8229..8966
                     /locus_tag="VVL53_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183459.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGENR"
     gene            8963..10654
                     /locus_tag="VVL53_001289"
     CDS             8963..10654
                     /locus_tag="VVL53_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4183460.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            10770..11333
                     /locus_tag="VVL53_001290"
     CDS             10770..11333
                     /locus_tag="VVL53_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU4183461.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKF"
     gene            11355..>14623
                     /locus_tag="VVL53_001291"
     CDS             11355..>14623
                     /locus_tag="VVL53_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU4183462.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSITNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEQAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TVNQSIEQPKVETPAVEKQTEPTEEPKVEQAGEPVAPREDEQAPTAPVEPEKQPEVPE
                     EEKAVEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLET
                     AKGVYASEPVKQPEVNSETNKLKTAIDALNVDKTELNNTIADAKTKVKEHYSDRSWQN
                     LQTEVTKAEKVAANTDAKQSEVNEAVEKLTATIEKLVELSEKPILTLTSTDKKILERE
                     AVAKYTLENQNKTKIKSITAELKKGEEVINTVVLTDDKVTTETISAAFKNLEYYKEYT
                     LSTTMIYDRGNGEETETLENQNIQLDLKKVELKNIKRTDLIKYENGKETNESLITTIP
                     DDKSNYYLKITSNNQKTTLLAVKNIEETTVNGTPVYKVTAIADNLVSRTADNKFEEEY
                     VHYIEKPKVHEDNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTG
                     KLLSSEGKQFAITELEHPLFNVITNATINNVNFENVEIERSGQDNIASLANTMKGSSV
                     ITNVKITGTLSGRNNVAGFVNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAGTNYRG
                     IVRKAYVDATITGNKTRASLLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVSNPVEV
                     GAIASKTWPVGTVSNSVSYAKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGYSSGNR
                     SFRKSKTFTKLTKEQADAKVTTFNITADKLESDLSPLAKLNEEKAYSSIQDYNAEYNQ
                     A"
BASE COUNT         4478 a         2676 c         3235 g         4234 t
ORIGIN      
        1 tcctgcacgc cagaagaatt tgatgaattt aggatagtaa aagctgcgtt tttttaaaag
       61 gaagaaaaag acgagaataa tggctaggaa cgagagggct agccccagtc tagggaggaa
      121 attatgggta aaggttttag ctgtaatcaa gtaatactca aataccaaga ctggaaaagc
      181 caaatccgca aagtttcgtc ctagtttttt taatctaaaa agtttggtta ggataatgca
      241 gacgactaag gtcagtatca ataataaaat agatgctaat ttcattaaaa tcatacccat
      301 attgtatcat aaaaaaaggc taatggaaac gagaaacaga ggaatttata ggaaagtcag
      361 gcttttgaga ttgtgggtga tttttgttat aatgaaagtt ataaaatctt ataatacctt
      421 gaatgattat tatcgaaaac tgtttggaga aaagactttt aaagtcccta ttgatgcggg
      481 atttgactgt cccaatcgtg atggaactgt ggctcatgga ggctgtactt tttgtacggt
      541 ttcaggttct ggagatgcta ttgtagcccc agatgcgcct atccgtgagc aattttataa
      601 ggaaattgac tttatgcacc gtaagtggcc ggatgttcag aagtatctgg tttattttca
      661 aaattttacc aacacccatg aaaaggtgga agttattcga gagcgttatg aacaggctat
      721 caatgagcca ggtgtggtag gtatcaatat tggaacgcgc ccagactgtt taccagacga
      781 aaccatcgaa tatttggctg agttatcgga gtgcatgcat gtgatggtta aattgggctt
      841 gtagactact tatgaagcaa cttctgacct gattaaccgt gcgcattctt atgaattgta
      901 ggtggaaacg gtcaagcgtt tgagaaagta tcccaagatt gagattgttt cccatttgat
      961 caatggtttg cctggtgaaa cccatgagat gatggttgaa aatgtccgcc gttgtgtcac
     1021 gaataacgat attcaaggga ttaaactgca tttgctccat cttatgacaa atactcgtat
     1081 gcaacgagat taccatgagg gacgtttaca attgatgagt caggacgaat atgtcagggt
     1141 catctgtgac caactggaaa tcattcccaa acatatcgcc atccatcgaa ttacaggaga
     1201 tgcgcctagg gatatgctgc ttgggcttat gtggagcctc aataaatggg aagctctcaa
     1261 cagcattgag atggagatgc gacgtcgtgg aagtgttcaa ggatgcaagg ctgtaaaaca
     1321 ggagtttgaa aatgaaaaga ccacttgaga tggcacatga ttttttggct gaggtcgtga
     1381 caaaagagga tgtcgtagtg gatgcgacta tgggaaatgg tcatgacacg ctttttttag
     1441 ccaagctagc caagcaagtc tatgcctttg atattcagaa gcaagccttg gaaaagaccc
     1501 aagagcgttt gcatcaggct ggcttgacaa atgcccagtt aatcttgcaa ggccatgaga
     1561 cactggacca gtttgtgaca aaagctaagg cagggatttt taatttgggc tatttgccgt
     1621 cagctgataa gtctgtcatc acccgaccgc agacaacgat tgaggcatta gaaaagctat
     1681 gtggcttact tgtcaaaggt ggacgaattg ctattatgat ttactatggt catgaaggag
     1741 gcgacctcga gagagatgct gtcttggatt ttgtgatcca gttgaaccaa caagagtaca
     1801 cagctgccat ttaccgaact ttaaaccaag tcaacaaccc gccgttttta gtgatgattg
     1861 aaaaattaga gagatacaga catggataaa caatacctac acgaaaagct ggatgtcatg
     1921 cgccagaatt ttgttgaatc aacccaccac gaacgagcgg tgggcgtgct agaccaagcg
     1981 catatgagca aaaaaatgct taaaatcaag aaaaaattag ttgctcttga aatggaacgg
     2041 tgccagagaa aaattgagca caaagattgt tccaagattg accaaaaaat caaagatcag
     2101 aaggagatat ttgaatcctg ttgtaaaaaa gattaaggag gacgtgcgtg gaattactga
     2161 tttacctcat tctattttta ctggttttga ttgtctcaag tacaaccaat aagctcctgc
     2221 cctttttgcc tctccctttg gtgcagattc ttttgggaat tgtgattggt ctctttttac
     2281 ccaatactga ctttcacctt aatacggagt tgtttttggc actggttatc ggccccttgc
     2341 ttttccgaga ggctgaagaa gcagatgtta cggctatttt aaaacactgg cgaatcattg
     2401 tttatctcat atttccagtg atttttatct cgaccctgag tttgggtggc ttggcccatc
     2461 ttctttggtt cagccttccc ttggcagctt gcttggctgt tggggcagcc cttggtccta
     2521 cggacttggt ggcctttgcc tctctttcgg agcgttttag ctttcctaag cgcgtgtcca
     2581 atattcttaa gggcgaagga ctcttgaatg atgcttctgg tttggtggct tttcaggtag
     2641 ctttgacagc ttggacaact ggagcttttt ctctggggca agctagcagt tcgctcatct
     2701 tttcaatcct aggcggtttt ttaattggat ttttaacagc catgaccaac cgcttcctcc
     2761 ataccttctt gctaagtgtg cgcgcaacgg atattgccag tgaactttta ttagaattga
     2821 gtttgcctct agtgaccttc tttctggcag aagaagtcca tgtttcaggt attattgccg
     2881 tcgtagttgc tggaatttta aaggcaagtc gcttcaagaa aatcacgctc ctcgaagccc
     2941 aagtggatac ggtgaccgag acggtctggc atacagtgac ctttatgctc aacggttctg
     3001 tctttgtgat tttagggatg gagttggaaa tgatagcaga acctatcttg accaatccaa
     3061 tctataatcc tctactttta ttgctatctc tcatcgccct tacctttgtc ctctttgtca
     3121 ttcgttttat tatgatctat ggctattatg cctatagaac ccgacgcctc aagaaaaagc
     3181 taaataagta tatgaaggac atgtttctct tgaccttttc aggtgttaag ggaacggtgt
     3241 cgattgctac gattctcttg ataccaagta atctagaaca ggagtatcct ctcttgcttt
     3301 tccttgttgc aggtgtgacg cttgtcagct ttttaacagg tctcttggtc ttgcctcatc
     3361 tttctgatga agaggaagaa agcaaggatt atctcatgca tatcgccatt ttgaatgaag
     3421 taacgctaga gttggaaaaa gagttggaag acaccagaaa taaacttccc ctctatgcgg
     3481 ctattgacaa ttatcatgga cgtattgaaa atctcatttt aagccaagaa aaccaggatg
     3541 atcaagaaga ctgggctgct ttgaaactct tgattcttag tattgaaagt gatggtttgg
     3601 aacaggccta tgaagagggg aacattagca atcgtgctta ccgagtttac caacgttatc
     3661 tgaaaaatat agaacaagga atcaatcgta aacttgcctc aagactgacc tattattttc
     3721 ttgtttcctt gaggatttta cgttttctcc ttcatgaagt ttttactctt ggaaagacct
     3781 tccgtagctg gaaggacaag gagcaaagcc gtctccgtgc tcttgattat gaccaaattg
     3841 cagaactcta tcttgccaat acagagatga ttattgaaag tttggaaaac ctgaagggag
     3901 tctacagacg ctctttgatt agttttatgc aggagtctcg tcttcgagaa acagctatta
     3961 tcagcagtgg tgcctttgtc gaacgggtta tcaatcgtgt caaacccaac aatatcaatg
     4021 aaatgctgag aggctattat ctggagcgca agttgatttt cgaatacgaa gaaaaacgat
     4081 taattacaac taagtatgcc aagaaattac gacaaaatgt aaataactta gagaactatt
     4141 ccttgaagga agctgccaat accctgccgt atgatatggt ggaattggta agaagaaatt
     4201 agttaatact ctttgaaaat ctcttcaaac cacgtcagcg tcgccttacc gtatgtatgt
     4261 gactgacttc gtcagtctta tctacaacct caaaacagtg ttttgagcag cctgtggcta
     4321 gcttcctagt ttgctctttg attttcattg agtataagat tataagtgaa ggagtgtgac
     4381 atgaaaaagt ggtggaaaga gctgatagac aagcctttat taaaagcttt tttgcattat
     4441 tatcaagcat cagatagtga gttgaccagt gtcgcagtag cctactattg gttgatttcg
     4501 attttccccc tgcttttggt ggtggtcaat atcctcccct attttcagat tcctgtggga
     4561 gaatttctgg gctttatgaa agacgtcctg cccccaagcc tctatgaagg tgtggaaaaa
     4621 atagccagag aagtcttgac gcaaccttca acaggtttat tgagtttttc tgttttatcg
     4681 gcgttatgga gtttttcaaa atctatgaat ttcttgcaaa aagcttttaa caaggcttat
     4741 ggcgtcgaaa agagtcgtgg acttatttcc catcagatgc taagtctcct agtcagtttt
     4801 ggcttgcagc tcctctttgc gtttgccttg tttttgatct tatttggtca gatgattttg
     4861 gctttacttg ctcattattg gacaaaagat ggcattatct atcaggcttt acaagggttg
     4921 gcaggtcctc tgatttacgc cctcctcttt gctatcttgg tcatgcttta ttattttctt
     4981 cccaatctat ctaacaggaa aattagctat accctaccag gcagtgcctt tgttctctta
     5041 gtgattttag gtttactaac tctgttctct agttatctca attactatgt ccatcatttg
     5101 gtggatgtcc gaattttagg ttccgttctt cttgttgtta tgatgttttg gtttatcttg
     5161 attgctaaaa ttgttatcct aggcgcggtg atcaatgcca gtatgcagag tttgaaagat
     5221 ccggtcttta aaaaagacta actatttatc cattacaaaa gcgcatagta tcaaggcttt
     5281 atagaatctt gatactatgc gcttttgtgc tttgacagtt ttttgtatag tttttgtcca
     5341 agatttctaa atcatctgat taaattccta ttttccccta tcattgtccc tgttttttcg
     5401 aaaatggaag gagtataata aatctatcaa taaaatgata ggaggaactt tatgggtcat
     5461 attttctttt ttctaagtgt ctttttggca gggattctat ccttcttttc tccttgtatc
     5521 ttacctttgt taccggtcta tacaggagtg ttactagatg ataaggatgg tgctcaggct
     5581 tctagcggca aattttcaat ctcagttact agtttattac gaactctggc ctttatagca
     5641 ggaatttcct ttatatttat tttgttgggc tatggagctg gttttttagg cgatttgctt
     5701 tatgcttctt ggttccaata tcttactggg gcaattatta tccttcttgg tttgcaccaa
     5761 atggagattc tacactttaa ggggctttat aaggaaaaga ggctacaact gcaaggacag
     5821 gggcaaaatg gtaagggcta tagtcaggca tttttattgg gcttgacctt tagttttgct
     5881 tggacgcctt gcgtggggcc ggttctgggg tctgttttgg ccttggcggc ttcaggtggt
     5941 tcaggagctt ggcagggagc tggtctcatg ttggtgtata cgctgggctt ggcgctacca
     6001 ttcttgcttc tagctctgac ctctagttat gttttgaaac atttccgaaa acttcatccc
     6061 tatctcggaa tcctcaaaaa agtgggtggt tttctcatta ttgtgatggg cttcttggtt
     6121 ctgtttggaa atgcttcaat tttaagtcaa ttatttgaat aaaatggaaa ggaatatcaa
     6181 tatgaaaaaa tggcaaacat gtgttcttgg agcaggttcg ctcctttgtt tgacggcttg
     6241 ttcaggcaag tccgtgacta gtgaacacca aacgaaagat gaaatgaaga cggagcagac
     6301 agctagtaaa acaagcgcag ctaaagggaa agaggtggct gattttgaat tgatgggagt
     6361 agatggcaag acctaccgtt tatctgatta caagggcaag aaagtctatc tcaaattctg
     6421 ggcttcttgg tgttccatct gtctggctag tcttccagat acggatgaga ttgctaaaga
     6481 agctggtgat gactatgtgg tcttgacagt agtgtcacca ggacataagg gagagcaatc
     6541 tgaagcggac tttaagaatt ggtataaggg attggattat aaaaatctcc cagtcctagt
     6601 tgacccatca ggcaaacttt tggaaactta tggtgtccgt tcttacccaa cccaagcctt
     6661 tatagacaaa gaaggcaagc tggtcaaaac acatccagga ttcatggaaa aagatgcaat
     6721 tttgcaaact ttgaaggaat tagcctagga ggcgtcttat gaatgataag ttaaaaatct
     6781 tcttgttgct aggagtattt tttctagcca taaccggttt ctatgttcta ttgatacgaa
     6841 atgcagggca gacagatgcc tcgcaaattg aaaaggcggc agttagccaa ggaggaaaag
     6901 cagtgaaaaa aacagaaatt agtaaagacg cagacttgca cgaaatttat ctagctggag
     6961 gttgtttctg gggagtggag gaatatttct cacgtgttcc cggggtgacg gatgccgttt
     7021 caggctatgc aaatggtaga ggagaaacaa ccaagtacga attgattaac caaacaggtc
     7081 atgcagaaac cgtccatgtc acctatgatg ccaagcaaat ttctctcaag gaaatcctgc
     7141 ttcactattt ccgcattatc aatccaacca gcaaaaataa acaaggaaat gatgtgggga
     7201 cccagtaccg tactggtgtt tattacacag atgacaagga tttggaagtg attaaccaag
     7261 tctttgatga ggtggctaag aaatacgatc aacctctagc agttgaaaag gaaaacttga
     7321 agaattttgt ggtggctgag gattaccatc aagactatct caagaaaaat ccaaatggct
     7381 actgccatat caatgttaat caggcggcct atcctgtcat tgatgccagc aaatatccaa
     7441 aaccaagtga tgaggaattg aaaaagaccc tgtcacctga ggagtatgca gttacccagg
     7501 aaaatcaaac agaacgagct ttctcaaacc gttactggga taaatttgaa tccggtatct
     7561 atgtggatat agcaactggg gaacctctct tttcatcaaa agacaaattt gagtctggtt
     7621 gtggctggcc tagttttacc caatccatca gtccagatgt tgtcacctac aaggaagata
     7681 agtcctacaa tatgacgcgt atggaagtgc ggagccgagt aggagattct caccttgggc
     7741 atgtctttac ggatggtcca caggacaagg gcggcttacg ttactgtatc aatagcctct
     7801 ctatccgctt tattcccaaa gaccaaatgg aagaaaaagg ctacgcttat ttactagatt
     7861 atgttgatta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtatg
     7921 tagggttact gacttcgtca gttttatcta caacctcaaa gctatacttt gagctgactt
     7981 cgtcagtttt atttacaacc tcaaagctat actttgagct gacttcgtca gttttatcta
     8041 caacctcaaa gctatacttt gagctgactt cgtcagtttt atttacaacc tcaaagcggt
     8101 actttgagaa acctgtggct atcttcctag tttacttttt gatttttatt gagtataaga
     8161 ggtctttcct aagcagttag aggagaattt tgctatactg atactagtaa gtggtaaagg
     8221 agcagagcat gacctacaca atcttaatcg tagaagatga atatctggta agacaaggtt
     8281 tgactaaact ggtcaatgta gcagcctacg atatggaaat catcggtcag gctgaaaatg
     8341 gaaggcaggc ttgggaattg atccaaaagc aggtgccaga tatcatttta accgatatca
     8401 acatgcctca tctaaatggc atccagttgg ccagtctggt acgagaaacc tatcctcagg
     8461 ttcatttggt ctttttaaca ggttacgatg attttgatta tgccttgtct gctgtcaaac
     8521 taggtgtgga cgactacctg ctcaaaccct tttctcgtca ggatattgag gaaatgttgg
     8581 ggaaaatcaa acaaaaacta gacaaggaag agaaagaaga gcagttacaa gatttattga
     8641 ctaataggtt tgaaggaaac atggcccaga aaatccagtc tcatctggct gatagccaat
     8701 ttagtttaaa gtctttagcc agtgacttag gttttagtcc gacctatctg agttccttga
     8761 ttaagaaaga gttgggcttg ccttttcagg attatctggt gagagaacgt gttaaacaag
     8821 ccaagctctt gcttttaact acagatttga agatttatga gatcgcagag aaggttggtt
     8881 ttgaagatat gaactatttt acccaacgtt ttaagcagat tgcaggtgtg acacctcgtc
     8941 agtttaagaa gggagaaaac cgatgaagcg ttcttctctt ttagttagaa tggttatttc
     9001 catctttctg gtctttctca ttctcctagc tctggttgga actttctact atcaatcaag
     9061 ttcttcagcc attgaggcca ccattgaggg caacagccaa acgaccatca gccagactag
     9121 ccactttatt cagtcttata tcaaaaaact agaaaccacc tcgactggtt tgacccagca
     9181 gacggatgtt ctggcctatg ctgagaatcc cagtcaagac aaggtcgagg gaatccgaga
     9241 tttgtttttg accatcttga agtcagataa ggacttgaaa actgttgtgc tggtgaccaa
     9301 atctggtcag gtcatttcta cagatgacag tgtgcagatg aaaacttcct ctgatatgat
     9361 ggctgaggat tggtaccaaa aggccattca tcagggagct atgcctgttt tgactccagc
     9421 tcgtaaatca gatagtcagt gggtcatttc tgtcactcaa gaacttgttg atgcaaaggg
     9481 agccaatctt ggtgtgcttc gtttggatat ttcttatgaa actctggaag cctatctcaa
     9541 tcaactccag ttggggcagc agggctttgc cttcattatc aatgaaaacc atgaatttgt
     9601 ctaccatcct caacacacag tttatagttc gtctagcaaa atggaggcta tgaaacccta
     9661 catcgataca ggtcagggtt atactcctgg tcacaaatcc tacgtcagtc aagagaagat
     9721 tgcaggaact gattggacgg tgcttggcgt gtcatcattg gaaaagttag accaggttcg
     9781 gagtcagctc ttgtggacct tgcttggggc cagtgtcaca tctcttcttg tctgtctctg
     9841 cttagtgtgg ttcagtctta aacgctggat tgctcctttg aaggatttga gagaaaccat
     9901 gttggaaatt gcttctggtg ctcaaaatct tcgtgccaag gaagttggtg cctatgaact
     9961 gagagaagta actcgccaat ttaatgctat gttggatcag attgatcagt tgatggtagc
    10021 tattcgtagc caggaagaaa cgacccgtca gtaccaactt caagcccttt cgagccagat
    10081 taatccacat ttcctctata acactttgga caccatcatc tggatggctg aatttcatga
    10141 tagtcagcga gtggtgcagg tgaccaagtc cttggcaacc tatttccgct tggcgctcaa
    10201 tcaaggcaag gacttgattt gtctctctga cgaaatcaat catgtccgcc agtatctctt
    10261 tatccagaaa caacgctatg gagataagct ggaatacgaa attaatgaaa atgttgcctt
    10321 tgataattta gtcttaccca agctggtcct acaacccctt gtagaaaatg ctctttacca
    10381 tggcattaag gaaaaggaag gtcagggcca tattaaactt tctgtccaga aacaggattc
    10441 gggattggtc atccgtattg aggatgatgg cgttggcttc caagatgctg gtgatagtag
    10501 tcaaagtcaa ctcaaacgtg ggggagttgg tcttcaaaat gtcgatcaac ggctcaaact
    10561 tcattttgga gccaattacc atatgaagat tgattctaga ccccaaaaag ggacgaaagt
    10621 tgaaatatat ataaatagaa tagaaactag ctaactccca gtcaattctg ggagttttat
    10681 gcgtaattgg gcaaactttc taggttgtct atcttgctaa aaacaagaaa aaacggtatg
    10741 atagaagagt gacaacagag gtaacagaga tgaacgagac acagattcaa agagaaacac
    10801 gtcaggtagt ggaagatgtt ttagaaaaga ccaatttgaa gcagggggcc ctttttgttt
    10861 taggtctatc ttctagtgag gtgctaggtg gtcatattgg gaaagaatcc agtcaagaga
    10921 ttggtgagct gatagtggag actatcttgg atattctagg tagtagaggt atccatttag
    10981 cagttcaagg ttgtgagcat gtcaatcggg ctctcgttgt tgaacgtcag gtggcagagc
    11041 agtttggtct ggaaattgtc agtgtccatc ctactcttca tgcaggaggt tcggggcagt
    11101 tggcagcctt taagtttatg caggatccag ttgaggttga atttatcaag gctcatgctg
    11161 gattggatat cggagacact gcaattggca tgcatgtcaa gcatgttcag gttccgattc
    11221 gccctatttt gagagagatt ggtcatgccc atgtaacggc actggctagt cgtccaaaat
    11281 taatcggagg tgcgcgtgcg cactatccgc aagatgctat taggaagttt tgagaatgaa
    11341 taaaggagat aaaagtgttt aaaaaagacc gtttttcaat tcgtaagatt aagggagttg
    11401 taggctctgt atttcttgga agccttttga tggctccttc tgtagtggac gcagccacct
    11461 atcactatgt aaataaagag attatttcac aagaagctaa agatttaatt cagacaggaa
    11521 agcctgacag gaatgaagtt gtatatggtt tggtgtatca aaaagatcag ttgcctcaaa
    11581 cagggacaga agcatctgtt ttgacagctt ttggtttgct gactgttggg agcttgcttt
    11641 taatctacaa gagaaagaaa attgctagcg tctttctagt tggagctatg ggattggtag
    11701 ttcttcctag tgcaggggct gtagacccag ttgcgaccct agcgctggct agtcgagagg
    11761 gtgttgttga aatggagggc tatcgctatg ttggttatct atcaggtgac atcctcaaaa
    11821 cgcttggctt ggacactgtt ttagaagaaa cctcagcaaa acctggagag gtgactgtgg
    11881 tcgaagttga gactcctcaa tcaataacaa atcaggagca agctaggaca gaaaaccaag
    11941 tagtagagac agaggaagct ccaaaagaag aagcacctaa aacagaagaa agtccaaagg
    12001 aagaaccaaa atcggaggta aaacctactg acgacaccct tcctaaagta gaagagggga
    12061 aagaagattc agcagaacca gctccagttg aagaagtagg tggagaagtt gagtcaaaac
    12121 cagaggaaaa agtagcagtt aagccagaaa gtcaaccatc agacaaacca gctgaggaat
    12181 caaaagttga acaagcaggt gaaccagtcg cgccaagaga agacgaacag gcaccagtcg
    12241 agccagaaaa gcaaccagaa gctcctgaag aagagaaggc tgtagaggaa acaccgaaac
    12301 aagaagagtc aactccagat accaaggctg aagaaactgt agaaccaaaa gaggagactg
    12361 ttaatcaatc tattgaacaa ccaaaagttg aaacgcctgc tgtagaaaaa caaacagaac
    12421 caacagagga accaaaagtt gaacaagcag gtgaaccagt cgcgccaaga gaagacgaac
    12481 aggcaccaac ggcaccagtt gagccagaaa agcaaccaga agttcctgaa gaagagaagg
    12541 ctgtagagga aacaccgaaa ccagaagata aaataaaggg tattggtact aaagaaccag
    12601 ttgataaaag tgagttaaat aatcaaattg ataaagctag ttcagtttct cctactgatt
    12661 attctacagc aagttacaat gctcttggac ctgttttaga aactgcaaaa ggtgtctatg
    12721 cttcagagcc tgtaaaacag cctgaggtaa atagcgagac aaataaactt aaaacggcta
    12781 ttgacgctct aaacgttgat aaaactgaat taaacaatac gattgcagat gcaaaaacaa
    12841 aggtaaaaga acattacagt gatagaagtt ggcaaaacct ccaaactgaa gttacaaagg
    12901 ctgaaaaagt tgcagctaat acagatgcta aacaaagtga agttaacgaa gctgttgaaa
    12961 aattaactgc aactattgaa aaattggttg aattatctga aaagccaata ttaacattga
    13021 ctagtaccga taagaaaata ttggaacgtg aagctgttgc taagtatact ctagaaaatc
    13081 aaaacaaaac aaaaatcaaa tcaatcacag ctgaattgaa aaaaggagaa gaagttatta
    13141 atactgtagt ccttacagat gacaaggtaa caacagaaac tataagcgct gcatttaaga
    13201 acctagagta ctacaaagaa tacaccctat ctacaactat gatttacgac agaggtaacg
    13261 gtgaagaaac tgaaactcta gaaaatcaaa atattcaatt agatcttaaa aaagttgagc
    13321 ttaaaaatat taaacgtaca gatttaatca aatacgaaaa tggaaaagaa actaatgaat
    13381 cactgataac aactattcct gatgataaga gcaattatta tttaaaaata acttcaaata
    13441 atcagaaaac tacattacta gctgttaaaa atatagaaga aactacggtt aacggaacac
    13501 ctgtatataa agttacagca atcgcagaca atttagtctc tagaactgct gataataaat
    13561 ttgaagaaga atacgttcac tatattgaaa aacctaaagt ccacgaagat aatgtatatt
    13621 ataatttcaa agaattagtg gaagctattc aaaacgatcc ttcaaaagaa tatcgtctgg
    13681 gacaatcaat gagcgctaga aatgttgttc ctaatggaaa atcatatatc actaaagaat
    13741 tcacaggaaa acttttaagt tctgaaggaa aacaatttgc tattactgaa ttggaacatc
    13801 cattatttaa tgtgataaca aacgcaacga taaataatgt gaattttgaa aatgtagaga
    13861 tagaacgttc tggtcaagat aatattgcat cattagccaa tactatgaaa ggttcttcag
    13921 ttattacaaa tgtcaaaatt acaggcacac tttcaggtcg taataatgtt gctggatttg
    13981 taaataatat gaatgatgga actcgtattg aaaatgttgc tttctttggc aaactacact
    14041 ctacaagtgg aaatggctct catacagggg gaattgcagg tacaaactat agaggaattg
    14101 ttagaaaagc atatgttgat gctactatta caggaaacaa aacacgcgcc agcttgttag
    14161 ttcctaaagt agattatgga ttaactctag accatcttat tggtacaaaa gctctcctaa
    14221 ctgagtcggt tgtaaaaggt aaaatagatg tttcaaatcc agtagaagtt ggagcaatag
    14281 caagtaagac ttggcctgta ggtacggtaa gtaattctgt cagctatgct aagattatcc
    14341 gtggagagga gttattcggc tctaacgacg ttgatgattc tgattatgct agtgctcata
    14401 taaaagattt atatgcggta gagggatatt cgtcaggtaa tagatcattt aggaaatcta
    14461 aaacatttac taaattaact aaagaacaag ctgatgctaa agttactact ttcaatatta
    14521 ctgctgataa attagaaagt gatctatctc ctcttgcaaa acttaatgaa gaaaaagcct
    14581 attctagtat tcaagattat aacgctgaat ataaccaagc cta
//
LOCUS       DAVHEW010000047        14402 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVHEW010000047 DAVHEW010000000
VERSION     DAVHEW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.047"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..1288)
                     /locus_tag="VVL53_001292"
     CDS             complement(<1..1288)
                     /locus_tag="VVL53_001292"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU4183463.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1366..2613)
                     /locus_tag="VVL53_001293"
     CDS             complement(1366..2613)
                     /locus_tag="VVL53_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU4183464.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VVL53_001294"
                     /pseudo
     CDS             complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VVL53_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3778..4119
                     /locus_tag="VVL53_001295"
     CDS             3778..4119
                     /locus_tag="VVL53_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU4183465.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4177..4257
                     /locus_tag="VVL53_001296"
                     /pseudo
     CDS             4177..4257
                     /locus_tag="VVL53_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4463..6166)
                     /gene="ilvD"
                     /locus_tag="VVL53_001297"
     CDS             complement(4463..6166)
                     /gene="ilvD"
                     /locus_tag="VVL53_001297"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU4183466.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6399..7331)
                     /locus_tag="VVL53_001298"
     CDS             complement(6399..7331)
                     /locus_tag="VVL53_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU4183467.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7328..8185)
                     /locus_tag="VVL53_001299"
     CDS             complement(7328..8185)
                     /locus_tag="VVL53_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU4183468.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8189..9535)
                     /locus_tag="VVL53_001300"
     CDS             complement(8189..9535)
                     /locus_tag="VVL53_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU4183469.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(9548..9832)
                     /locus_tag="VVL53_001301"
     CDS             complement(9548..9832)
                     /locus_tag="VVL53_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4183470.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9836..11866)
                     /locus_tag="VVL53_001302"
     CDS             complement(9836..11866)
                     /locus_tag="VVL53_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU4183471.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            12058..13065
                     /locus_tag="VVL53_001303"
     CDS             12058..13065
                     /locus_tag="VVL53_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4183472.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13068..13268
                     /locus_tag="VVL53_001304"
     CDS             13068..13268
                     /locus_tag="VVL53_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU4183473.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13420..13602
                     /gene="rpmF"
                     /locus_tag="VVL53_001305"
     CDS             13420..13602
                     /gene="rpmF"
                     /locus_tag="VVL53_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU4183474.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            13618..13767
                     /gene="rpmG"
                     /locus_tag="VVL53_001306"
     CDS             13618..13767
                     /gene="rpmG"
                     /locus_tag="VVL53_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4183475.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14247..>14402)
                     /locus_tag="VVL53_001307"
     CDS             complement(14247..>14402)
                     /locus_tag="VVL53_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183476.1"
                     /translation="LWYYLNKSGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVN
                     ANGEWVG"
BASE COUNT         4651 a         3039 c         2487 g         4225 t
ORIGIN      
        1 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
       61 gtgacacttg cacttcttct cgggtttggt tgttcttgac cgtcacttgt ccgctttcga
      121 cttcgctctc tcctagggtg atgagggtct tagccgcaaa gacatcggct gacttgaact
      181 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
      241 cctgtaccaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
      301 ctagggcgtt ttcgataggg agggtcacac cttgcttttc aaggatgaga agcaggcgct
      361 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaagtaa gcaaccaaac
      421 catcgtagcg accacccgca cagacggtca ggtcattgcc ctcaatctct gtgataaact
      481 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
      541 ctactccaag attttccaac atctgacgca cagcatcaaa atgagcttgg ctttcttcat
      601 caagaaagtc caagatagac ggcgcattct ctactgccac cttgtcttct ttttccttag
      661 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
      721 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
      781 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
      841 ctgccatagc gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
      901 caatctggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
      961 ccatgtagta gaacttgctt ggcttttgca cttctggggc gaaaagttta ttttccacat
     1021 aggaacggac aacgggtgca gttccttctg gacggagggt aatatgacgg tcacccttgt
     1081 cataaaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcgactga
     1141 taacctcgta atgctcaaaa ataggcgtgc gcacttctgc atagttgtag cgtttgaaaa
     1201 tctcacgggc aaagccctca acgtactgcc acttagcaga ctcagcaggt aaaatatcct
     1261 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
     1321 ttaccataaa tagagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
     1381 gagattaaga attgtcaaaa aaatagctag caaggaaaga ccaacaaata gcatccaagt
     1441 caactgtata ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
     1501 ggtaaacaat agacctaaaa aattactagt acgagctaga acctctggag ctagattttt
     1561 catgagcatg gcactaatct ttggttgaac tttaccagac acatacagag taaagaagag
     1621 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaagtcc
     1681 tacggtctcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
     1741 aaggctactg atgatgactg atactaaaac acagaattga ttgataaata gtgcctctgt
     1801 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
     1861 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc
     1921 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga
     1981 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
     2041 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
     2101 gagagaaaaa ttgttgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat
     2161 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcagat catcttcagc
     2221 tactttttcc ttaataagag gcatacgcag gccacctgca aaatcactga tgatatcact
     2281 aatgacattg atcaaacaca ggctagaaaa ggcaaagaga ctagcttgct gaacaactag
     2341 ggctgctaga aaaaatagaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt
     2401 gtccctcgtg taatctgccc gaatccctgc aaaaactgta aagagggtcg gaagaatcat
     2461 gacaatattc gccatagcaa cagcaaaaga tgcttgtgac aaggtcgatg catagacgat
     2521 aaagaccagg ttgaaaatcg aaacaccaaa agcattgaag aagcgtgata aagtcaaaat
     2581 tctataagct ggatttctaa acaatagttt catagataat ctccctcctt gttctaagtt
     2641 ctacatcaag aatagcttta gtatagcact tatgaatttg cgaaacgatt cttttgaata
     2701 tatgcaacaa acaaaatgag caaaaaaacg atagaacttt tgtaatgacc tgagagaggg
     2761 caacagttct atcgcttcaa attttttctt ggtttgcaga tattcaagaa tcgggagttt
     2821 ttctatagta ttcggcagat ttattacagc caagcatctc aaaaatacgg acagcatcct
     2881 ccatcttttt ctggccttcc ttgactctac cttgcttgct atcaaggaga ccttctgccc
     2941 acagataaac aattcggaaa taggtctcat tttccttgta gaaatgctct tcgataacac
     3001 gtttaaaata ataggcattg gtaaattctt cacactcaat actagctaaa aagccattca
     3061 atagtatagt atgaaaaagg tttcgattgc cagacatttc cattagaaaa tcagatttac
     3121 gtaccatttc tcgtacatat ctagtaaaaa gagaaacaga taaaaatgga gaactgactg
     3181 aaaataaatt gagttcatag attccccaga tctcggtaga aaacaaataa tcatgaagga
     3241 cttttccttc ctctgctgtt aagtctaccc tttcatctat gctctttata taagacttga
     3301 taataatggc atttagaata tgtttctgtt tgttgtgaga atgggcatgc ttttatactc
     3361 cctgcgatat aagtcctcaa gaggtgctat attctttggt tccaagacat ctgtaatttc
     3421 ttttctcaac tcagaatctg tatcatactg gaaacctctt gccagaaaga ggatctcctc
     3481 cacactggca gatatatttt ccagagcaaa tagaaacttt tccaccgaaa gctcactctg
     3541 acctgtttca aaacgggaca acatagacgg cgaaaattgt cctccggttg cttgtctcag
     3601 tgagatattt cttgactctc gtaattgtct aaagactttt ccaatctgct ccatagactt
     3661 ccccttgatt ccgtattttc ttcattttat catatttttc agaaaattca tcaaaaactt
     3721 gccaaattgt cagaattatg agaaaataga ggatatttat cacgtggagg gactgctatg
     3781 agagacgata tcaaaatcaa tgaccgtgct ttggccttgc aagaccaaat tatcgaaaaa
     3841 ctagagaaag tttttgatac agatgtggaa ttggatgttt acaatctagg tctgatttat
     3901 gaaatcaatc tggatgaaac ggggctctgc aagattgtca tgaccttcac cgatactgcc
     3961 tgtgattgcg ccgaaagcct gcctattgaa atcgtggcag gtctgaaaca aatcgagggt
     4021 atcaaagata tcaaggttga agttacctgg tcgcctgctt ggaaaatcac acgaatcagt
     4081 cgctatggcc gtattgccct tggactacca cctcgttaag cagaccaatc acttttaaag
     4141 atgaaaaaag caagccgaaa atggcttgct ttttgatact caatgaaaat caaagggcaa
     4201 actagaaaac tagccgcagg ttgcccaaaa cactgttttg aggttgtgga tagaactgac
     4261 gaagtcagcc caaaacactg ttttgaggtt gtagatagaa ctgacgaagt cagctcaaaa
     4321 cactgttttg aagttatgga tagaactgac gaagtcagct caaaacactg ttttgaggtt
     4381 gtagatagaa ctgacgaagt cagtaaccat acctacggca aggcgacgtt gacgtgattt
     4441 gaagagattt tcgagtatga gtttattttt tacctgactt gtccatattc cagaagtctg
     4501 tcacggctcc gcgtgaagca gatgatacga tgtgggcata tttaccgagg acaccacggc
     4561 tgtaaagtgg tggcaaggtt gtttctgcct tgcgtttttc aagttcttct tcggatacgg
     4621 ccatggaaat ttctttggta tcttggtcaa ccgtaacgat atcgccggta cggagatagg
     4681 caattggtcc accatcctga gcttcaggag cgatatgtcc aacaaccaga ccataagtac
     4741 caccagagaa acgtccgtcc gtcaagaggg ccaccttatc tccctgacct ttaccaacaa
     4801 tcattgaaga aagtgacagc atctcaggca taccaggacc acctttaggt ccaacaaaac
     4861 gaacaacgac tacatcgcca tcaacgattt catctgtcag aacggcctga atcgcatctt
     4921 cttctgagtc aaagacctta gctggcccaa cgtgacgacg cactttaaca cctgatacct
     4981 tggcaactgc accgtcagga gcaaggttcc cgttcaagat gataagcgga ccatccgcac
     5041 gttttggatt ttcaagtggc atgataactt tttggcctgg agtcaagtct gcaaagtcag
     5101 ccaagttttc agctacagtc ttaccagtac atgtgatgcg atctccgtga aggaaaccat
     5161 ttgccaacaa gtacttcata accgcaggga caccaccgac ttcgtagagg tcttggaaga
     5221 catactgacc agatggtttc aagtcggcca agtgaggcac acgttcttga atcgtattga
     5281 agtcctcaag tgacaagtca acatttgcgg catgggcaat ggcgagcaag tgaagagtgg
     5341 cgtttgtaga accaccgaga gccatcgtta cagtaatagc atcttcaaag gcttcacgag
     5401 tcaagatatc tgatggtttg agaccaagtt ccaacatctt aacaacagca cgtcctgctg
     5461 cttcgatatc ttctttctta tcagctgatt cagctgggtg agaggatgac cctggcaaac
     5521 tcatccctag aacttcgata gcagttgcca tggtattagc agtatacata ccaccacagc
     5581 caccagggcc agggcaggca ttacattcaa gacgtttcac gtcctcagct gtcatgtcac
     5641 cgtggttcca ttttccgata ccttcaaaga cagaaaccaa gtcgatatct ttaccatcaa
     5701 gatttcccgg tgcaatagtt ccaccatagg cgaaaatagc tgggatatcc atattagcaa
     5761 tagcaatcat agatccaggc atgttcttgt cacagccacc gatagcgaca aaggcatcca
     5821 cgttgtgacc actcatagcc gcctcgatgg agtccgcgat gatgtcacga gatgttagag
     5881 agaaacgcat accaggcgtt cccatagcga tcccgtctgc tacggtaatg gttccaaact
     5941 gtacaggcca agcgcctgca gatttgacac cttctttagc cagtttcccg aaatcatgca
     6001 agtgaatgtt acatggtgta ttttccgccc aagtcgaaat cactcccaca atcggtgttt
     6061 caaagtcctt atctgtcata ccagtcgcac gaagcatagc acggttaggt gatttaacca
     6121 tgctgtcata aatgctactg cggtgacgtt tatctaattc agtcatctta tccctcccat
     6181 ttcagttttt actattatag cacaattttc gcatgaagaa cagaataaaa atcttgaatt
     6241 ttcagaaaat tctatacaca tgtgaaatat ttaaaattaa aaacaacaaa gcggattagt
     6301 gcactttctg atgaccagaa tatgcttttt aatccgcttt ctttaaataa cgtactgtaa
     6361 tttttacaga aattctttca gataagtgta tttaacatct atcttgcatt ataaatttct
     6421 agaaccttct cttttatatt cgattcactc aaaccatact cattaagaag ataatccatt
     6481 ttccctactt gaccgaatct ttcttgaaca cccatccgat gaatttttgt cattccatca
     6541 tcagagaata attcacataa agaactgcca attccaccta tctgattgtg gttttctaca
     6601 gtaaatatag tttttccact taacattgtt tttatctgtt ctggtatcgg tttgattcta
     6661 aataaatcta tcacacttac tgaataacct aatttagaca gttcatctgc aactcgaata
     6721 cttggagcaa ccattatacc agaagcaacg attacaagat cttcaccatg ccttaactca
     6781 atgtagcctt tagaaaaatc ttctccacct tgatacacag gaactggagc ttttctaatt
     6841 gttcgaatat attttagtcc ttttaagtct aatgtctggt tcaatatttc acgaaattgg
     6901 atatcatcag ttgcttcaaa aatgatcgat ttaggaatta aacgtaacaa tccaatttct
     6961 tcaaatggca tatgtgtccc accattcatt tctgcagtta ctcctgcatc tgatccaatc
     7021 acagtggcat ccaattgtgc gtatccaaga gaaataaata attgatcaaa tactcttcgt
     7081 gaagcaaaag gaccaaatgt atgaagataa ggtctaaacc cctgaataga caagcctgct
     7141 gcaaggccga ccatttctgc ttccataatc ccaacattca cataacggtc tccaaaatcc
     7201 ttttcaagat tattagtagc catcgaactt gacaaatcgg cttctaagac tactatatca
     7261 gaataacttt gattagcctc taaaaggaag tctctatata catgccgtaa ttctttcgtg
     7321 cttctcatca ttctgtttcc tccaattcct gacttaatct ttctacaact gaagttaaca
     7381 tttgtctctc ctctacagta gggcgaagat gatggttgga tttcatttct tccagctctt
     7441 gaaccccttg acctttaata gtatctaata caatgcactt aggtgatgaa ttatttgact
     7501 gttttaattg gacaatccct tcataaattt ctctaatatc tgaacccttg accctaatgg
     7561 attcaaatcc aaatgctgaa aatttttcta cgaaatcacc tggattacaa atatcctttg
     7621 taaaaccatc taattgtttt ttgttatcat caacaaatac aattaagttg gataactgtt
     7681 gatgagaagc aaactgtata gcctcccaac attgtccctc atttaactca ccatctccaa
     7741 caatagcgta agtataaaag ggactctttc ttattctctg accatatgca agtccagttg
     7801 caacactaat tccttgtcct aaagagcccg ttgtcatatc tatgcctggc gttagatttc
     7861 tatcaggatg agacggtaat ttggttccat ttgtatttaa agaatataag aattctttgt
     7921 caaagaaacc attcaaatag agtgtactgt atagagctgg tcctccgtga ccttttgata
     7981 atatgaaata atctctatct cgtgctgcaa atatttctgg agtcattggc attatttcac
     8041 cataaagcac cgctaaaact tctacgatag acagacttcc tccgtaatgt ccgaatccaa
     8101 gatgattcaa tgttctaaga gtatttaatc ggatgttagt cgcaaatttt cttaacccat
     8161 cttctctatt tttacttaaa atcatccctt attcctccgt tgcagatggc tttttaataa
     8221 aggatactcc aaacataact gctagaataa gaacaagacc aatcacaatg cctgcttgtg
     8281 agccaaattg atttaacatt cctaaaataa ttcctgatag accaaaatct gcatctgaga
     8341 aagttgatcc ttggaaacca agtcctccca aaactggcat taaaaagact ggaagaaaac
     8401 tgattaaaat accttgtaaa aatgctccaa tagtggctcc acgaacacca ccagatgcat
     8461 tcccaatgac acctgcagtc gctccacaga agaaatgagg cacaacacct ggtaagataa
     8521 caaccgttcc tgaagcaatc ataattacca tacttactaa accaccaaca aaactagaga
     8581 taaatccaat tagaactgca ttgggtgcat aagtataaac aatcggacaa tccaaagcag
     8641 gttttgaatt aggtacaaga cgctctgaaa tacctttaaa ggctggaaca atttcgccca
     8701 aaataaggcg aacacctgct aaaataacaa atacccctgc tgcaaattga cctgctaatt
     8761 gtaaagcata aactagacca cttgtaccac tactgatttc tttttctata tattctgacc
     8821 ctgcaaagat agctacaata atgtaaataa ctgccatgga taaagtaata ctaacagtac
     8881 tatcacgtaa aaaagctaaa ctctttggaa atttaatgtc ctctgttgat tttgatttgt
     8941 caccgataag gctaccagta aaaccactca accaatatcc caaagaactg aaatgaccta
     9001 aagctacctt gtcatttcca gttaattgaa ccatatattt ttgcacaaat gctggggaaa
     9061 tactcataat aataccgagt gctaatcctc ctagtaagat gagaggcaag ctagtaaagc
     9121 cagcaactga taaaatgacc gcaatcatac atgccatata tagagtgtgg tgccctgtta
     9181 aaaaaatata tttaaatcga gtaaaacgag cgattaagat attgaacacc atgcctgcaa
     9241 acataatcat tgcagtagct gagccatatg ttgttaaagc tacagctaca attgcttcat
     9301 tattcggcac aacgccagat aaatgaaaag catgctcaaa catggtacca aatggattta
     9361 aagaattttg tacaattcct gcaccaccag atacaactaa gaaaccaaca aaggtcttaa
     9421 ttccaccttt aataatatca ggtaatttct tcttctgaag aactaatcct aagattgcaa
     9481 ttaaagctac taaaatagct ggtgtactaa caatatccaa tatgaacttc atcatgacgc
     9541 tagcctccta tataagtcct ttttcttcac aaagtttagt aattaattct cgtagttcat
     9601 ccatatcaat aatactattt aagatacgaa catctccaag atgactagct gaatcagcta
     9661 gatcacgacc aacaatccaa atatcagctg catttggatc tgctccacct aaatcataat
     9721 gttcaacttc tacatccgaa acattcaaat cactcaatac agattcaata ttcatctgta
     9781 ccataaaact tgaacctaat cctgaaccac aagctgtacc aatttttaac attatctaat
     9841 cctcctgttt aattatcatt ttaatgtcat catagttttt tgatgatatt aaagtttgaa
     9901 catgattttt atctcttaaa attgttgtta aatgtgacaa agcctttaaa tgactctcat
     9961 tatcaatggc tgcaatacaa atcaacaatc ttacctcttg ttctggatta tccaataaat
    10021 aaatcggttc ttccaaaact aacattgaca ttcctatttc attcacacct tcatctggcc
    10081 gagcgtgagg aattgctact cccttcccta aattaataaa aggcccaaac tcttctactt
    10141 tttgaatcat tgcctcaggg tagttctcag ttatcttatc ttgatccaaa agcggtttag
    10201 ctgctaaacg aatcgcctcc ttccatccta atttttgcga actaacctga taggtttctt
    10261 tggtaatgag ttgttctagc actggtacaa tttcctttct atcatttttt tggtaaagat
    10321 aattctttaa cgccaatctt aattccaatt cttgtgtaat aattccatat cttttgacaa
    10381 tattcaggat ttgttcaatc tcaaaatccc catactctaa attcggaaaa tcttttaaca
    10441 ctaattctac tagttgtatt gcttgctctt cagtcatcat aaccgaaact agataatttg
    10501 gcttttctgt ctccaccttt atggtagaaa aaaccatatc atagtcacta ctagctttca
    10561 cctgtaaatc atcaatcttt gaggttccta taaactcaat ttgaggaaat aatgctaata
    10621 gattctcttt taacatcaat gaagaactaa caccattagg acaaatgatt gctgctttat
    10681 accatttttg aggcaaagta tctgctttct ttaaataacc tccgaaatgg ataacaaaat
    10741 atgctgtttc actatcaggt atgggattgt caatagcgtc catcaagggc atcaaagaat
    10801 ctttgactaa ttcaaataaa tcaggataat gttctttaac atgcaataca tattcatttg
    10861 aactaggtag gccgaacttt aatctatagt aagccggtat aaggtggcgg cgaagatttt
    10921 ctctcaatcc ttccctttgt ttaaaatgta acaaagaaat atcttccatt ctacttataa
    10981 tagcctctgt taattgatta aagtaaaccg gagcaacatc tacttcacct tcaaagcaac
    11041 ttgataataa aacggtgata tagcgataat catcctcaga aaacaccgta tctataattc
    11101 ccaaatcaac cactgtatcc aataaaatag tggttatatc ttgaataaca ggagatacta
    11161 atgtctctga aagacatact ctttcaacat ccctttgata cctacacaga atgaatacta
    11221 aaccgaaaag gtaaactttt aattgattaa caataggtac tagctgtagc ttctcataat
    11281 aatctttaac tacctgatca atcaaatcat aagttaatga atacccccaa ctggataaaa
    11341 cataatccaa accccaaatc cctatggagg attccagcaa ctcactaacc atttgaaaag
    11401 ctaagcggtg cttattccac tctgaaccgt gtaaagtata accttttgct ctactgtacc
    11461 ctagctccaa atcattatct aacataatct ttcttaatga ttgaatatca gataaggttg
    11521 tattcttact tactttcaaa aagtcttggt aatgactatt cgatataaaa tctaatcggc
    11581 aaaaagtgta aagatagatt aaagctaagc gagtcgactt tggtaaaacc aattcatccg
    11641 acttaataat atctgtcaaa gactgcttcg tacgatttga taaactatag cgaccttgct
    11701 ttttatccag cactatccct ttattagcta gataaggcac taaataatct attccttctt
    11761 tgacttcctt tataggtaag ctcaccttaa cagataattc atataatgat agctcacaat
    11821 gatccatcaa agtcatcaaa ataactagtg ctctataatc aaacatttct cttcctttct
    11881 aactacaatt atagtgtaaa aattataaga ataaaagagt atttgagcac aatataatct
    11941 caccttttaa aagaaattat tgggctgaaa gtttttagtg attatttttc aactttctat
    12001 tgactttata aattaaagtg atatcattta gatatcaaat aacaggaggt cattcttatg
    12061 actgaaaaac taatcaattc aaaaccaaat ggtgtattcg cattgattct cattgagttg
    12121 acaatcgtac ttggtatctt tatatttata atgggggttg gttcggaaaa catttttgga
    12181 attattatcg gacctttact aatcgtaatt gcaggtctag ctcatgctgg tttaaaagtt
    12241 gtcaaacctc aagaagctct ggttctgaca ctctttggta actatacagg taccatcaaa
    12301 gaacctggct tttactttgt caatcccttc agcgtagcag tcaaccctgc aaaccacact
    12361 cgacttggac aaagtggtga tgttagcaca aaatctcctt ttttaggagc taaatcatca
    12421 aatgacaatg atgtaaatct tgaaattggc aagaaacaga tttccctcaa agtcatgacc
    12481 ttgagcaatt ctcgtcaaaa aatcaatgat tgcttaggaa accctgtaga aatcggtatc
    12541 gcggtaactt ggagagttgt cgataccgct aaggcagtct tcaacgttga taactacaaa
    12601 gaatatcttt cattgcaatg tgatagcgcc ctccgtaata ttgtccgcat ctatccttac
    12661 gatgtgtctc ctaatgtgga tactacgggt gatgggcaag cagatgaagg tagtctccgt
    12721 ggctctagcg aaattgttgc taaccgtatt cgtgaagaaa tccaaagtcg tgttgaggat
    12781 gctggcttgg aaatccttga agcacgtatc acttacctag cttatgctcc agaaattgct
    12841 gccgttatgc ttcaacgcca acaagcatct gccattattg atgcacggaa gatgattgta
    12901 gatggtgctg taggaatggt tgaaatggca ctagaacggc tcaatgaagg ggaattggta
    12961 gaacttgacg aagaacgaaa agctgccatg gtttcaaatc tccttgtcgt tctttgtggc
    13021 aatcatgatg cacaaccaat tgtcaacaca ggaagtcttt actaaagatg gctgaagcta
    13081 aaaaaaagca gatccccctt cgactctcaa caaagttata cgctgcactc gcatcatggg
    13141 cagaagacga ctttcgttca gtcaatgggc aaatcgaata tctcctcaca gagtgtgtca
    13201 agcaacggaa gaaagacgga aaatacgtat cagaaaccat tgacgaacca tttgagattg
    13261 atatttaaca ccttctcctg tctgccgtgt cggataggag aattttttca tctttttttg
    13321 tcaagtaact ttactttaca aaaaaaatgt gttatcctag tatggttgat gaaaatcagt
    13381 agattgaatc gaatataaat acctaaagga gaaatcaaaa tggcagtacc tgcacgtcgc
    13441 acttcaaaag cgaagaaaaa caaacgtcgt acacactaca aagtaacagc tccatctgta
    13501 aactttgacg aaactactgg agattactca cgttctcacc gtgtatcact taaaggatac
    13561 tacaaaggac gtaaaatcgc taaagctgca tcagctgaat aatagaaggg agataccatg
    13621 cgcgtaaata ttacacttga acacaaagaa tctggtgaac gcttgtacct tacttctaaa
    13681 aacaaacgta acactccaga ccgccttcaa ttgaagaaat actcaccaaa acttcgcaaa
    13741 cacgttgtgt ttacagaagt gaagtaagca ttcaatacga atcaatacag aagaaaaacg
    13801 cttgatcagc gttttttctt tttgttaatt tcactatatt gcaagggaaa tcattcgaat
    13861 gactaccttt tttgaaataa gaatacggat tcggacaatt aaaagcaaga ggaactcaaa
    13921 tgtaagttgg ggttcctttt ttgtatattt ttcaaataac gcctctacac tatttgtagc
    13981 aaattcacca actacagttg tatcttagtt aaaataagtt ataatatgta agtgagtacc
    14041 agatatacca agacatcgtc accatctaag gtatattcaa aatacaaaag ttgaccaact
    14101 agatttctga atatccttat atatccattc ttaaaattgg tttaaatagc gtagtctttt
    14161 aaactagttt tgagaatcca aaaaatcttc ctacatatgt aagaagattt tttagttcag
    14221 aatgattaga tttagctaat ggatacctat cctacccact caccgttagc attgactcga
    14281 tagccatcgg gtgtagtcgt gtttactaat aaatctcctg aattataggt atagtaccat
    14341 ttaccagaaa ctgtaaacca accagtagcc attgaacctg atttatttaa gtaataccat
    14401 aa
//
LOCUS       DAVHEW010000048        14183 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVHEW010000048 DAVHEW010000000
VERSION     DAVHEW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.048"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            83..880
                     /locus_tag="VVL53_001308"
     CDS             83..880
                     /locus_tag="VVL53_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU4183477.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..1150
                     /locus_tag="VVL53_001309"
     CDS             1028..1150
                     /locus_tag="VVL53_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU4183478.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1172..2212
                     /locus_tag="VVL53_001310"
     CDS             1172..2212
                     /locus_tag="VVL53_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU4183479.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2383..3657
                     /locus_tag="VVL53_001311"
     CDS             2383..3657
                     /locus_tag="VVL53_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU4183480.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VVL53_001312"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VVL53_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU4183481.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLSPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VVL53_001313"
     CDS             4786..5088
                     /locus_tag="VVL53_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU4183482.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VVL53_001314"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VVL53_001314"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU4183483.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VVL53_001315"
     CDS             5962..7302
                     /locus_tag="VVL53_001315"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU4183484.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VVL53_001316"
     CDS             7280..7492
                     /locus_tag="VVL53_001316"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU4183485.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VVL53_001317"
     CDS             7489..8364
                     /locus_tag="VVL53_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU4183486.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VVL53_001318"
     CDS             8357..9172
                     /locus_tag="VVL53_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU4183487.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VVL53_001319"
     CDS             9165..9596
                     /locus_tag="VVL53_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU4183488.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VVL53_001320"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VVL53_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU4183489.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VVL53_001321"
     CDS             11272..12000
                     /locus_tag="VVL53_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU4183490.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VVL53_001322"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VVL53_001322"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU4183491.1"
                     /translation="MNLEELKKRQRKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13983..>14183)
                     /locus_tag="VVL53_001323"
     CDS             complement(13983..>14183)
                     /locus_tag="VVL53_001323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183492.1"
                     /translation="SKHSFEVVDKTDEVSSKHDFEVVDETDEVSSKHGSEVVDETDEV
                     SNHTYGKATLTWFEEIFEEYKN"
BASE COUNT         4186 a         2596 c         3373 g         4028 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcggaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt tttgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg acttgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgattgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttt tcgatattga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgtgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgagg gtccaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa acctcttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtggcgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caaccactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaact aaattcgttt
     2221 gtcaatcggt cttgaaaatg tagaagattt gattgaagac ttgcgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg cttcgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaacg atatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gagagtcagt atgccgaact cttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaagcagga actggccttg
     2521 gctgagcaaa aggccatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattac agagtcagat ccaaaacttt gatacagaaa aagaattggc caagaaagag
     2641 gttgaacaga caagccatga ggctctcttg gctaaggaca aggaagtaca gctcttagaa
     2701 aatcagttgg ctaccttgcg tttggagcat gaaaatcaac tacaaaagac cctttctgac
     2761 ctagaaaaag aacgggatca ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa
     2821 ttatctttgg cttctgttaa gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgacc
     3541 acatctgaaa accaactccg tttagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat taacttaaaa aaggaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaatcactc tgggctattc cacgaagact
     3901 gaggatgcta gtggggaagt ggtcgcagaa acccctgttt tgtctctctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta ctcagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac gtacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaagtcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctgat gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaatcggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggaacctcaa gatgtcttta
     5521 tcgttgaggg tattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctgccccaa
     6361 ttttctaaga gaattggtgt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggtaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttacca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttagagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagactcat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagattaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tagcagggca agttttggat atggagggcg aacaccagca cttgtctttg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ctgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattagaag aaattgccca gcagcttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 ttttcgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta agattcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaaa
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattaccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgatacttt tgcagacaag atttttagtt gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgcctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctga
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacggtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tctcaaaacc
    11341 tgggatggtc agccccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtcc
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcgtcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatatttgt tcacgcaggt
    11701 attgatttga ctttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacagag gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac gggaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggat
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aaatacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttatcca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat
    14041 gtatggttac tgacttcgtc agtttcatct acaacctcag aaccatgttt tgagctgact
    14101 tcgtcagttt catctacaac ctcaaaatca tgttttgagc tgacttcgtc agtcttatct
    14161 acaacctcaa aactgtgttt tga
//
LOCUS       DAVHEW010000049        13638 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVHEW010000049 DAVHEW010000000
VERSION     DAVHEW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.049"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(671..1519)
                     /locus_tag="VVL53_001324"
     CDS             complement(671..1519)
                     /locus_tag="VVL53_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4183493.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLVPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSKLTEITIEAEI
                     LVGKAF"
     gene            1671..1949
                     /locus_tag="VVL53_001325"
     CDS             1671..1949
                     /locus_tag="VVL53_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183494.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1951..4014
                     /locus_tag="VVL53_001326"
     CDS             1951..4014
                     /locus_tag="VVL53_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU4183495.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(4092..5348)
                     /locus_tag="VVL53_001327"
     CDS             complement(4092..5348)
                     /locus_tag="VVL53_001327"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU4183496.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5490..7955
                     /locus_tag="VVL53_001328"
     CDS             5490..7955
                     /locus_tag="VVL53_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU4183497.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     VIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENIGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPEKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            8020..8931
                     /locus_tag="VVL53_001329"
     CDS             8020..8931
                     /locus_tag="VVL53_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU4183498.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            9161..9859
                     /gene="dapD"
                     /locus_tag="VVL53_001330"
     CDS             9161..9859
                     /gene="dapD"
                     /locus_tag="VVL53_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU4183499.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGSVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9927..11057
                     /locus_tag="VVL53_001331"
     CDS             9927..11057
                     /locus_tag="VVL53_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU4183500.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            11069..11608
                     /locus_tag="VVL53_001332"
     CDS             11069..11608
                     /locus_tag="VVL53_001332"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU4183501.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLQHPFYQEAKVI
                     ATYLSFSHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTFFALLELQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11592..12269
                     /locus_tag="VVL53_001333"
     CDS             11592..12269
                     /locus_tag="VVL53_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4183502.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGL"
     gene            12283..12360
                     /locus_tag="VVL53_001334"
                     /pseudo
     CDS             12283..12360
                     /locus_tag="VVL53_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(12570..13511)
                     /locus_tag="VVL53_001335"
     CDS             complement(12570..13511)
                     /locus_tag="VVL53_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183503.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLVLGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDDKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAAEKAKESSSQSNSSGSASNESYNGSSNS
                     NVDYSSSEQTNGYSNNYGGQDYSGSGDSSTNGGSSEQYSSSNSNSGANNVYRYKGTGA
                     DGYQRYYYKDHNNGDVYDDDGNYLGNFGGGIAEPSQR"
BASE COUNT         3864 a         2453 c         3038 g         4283 t
ORIGIN      
        1 ttactctcaa tcatcaagaa ttctcttttc gtggtagcgt ttggggtctg gtactggcct
       61 tatatcactt actattcatt gataagtttg ttatatcgaa tcgaaaataa agattagagc
      121 tatgcttgac tgtgtacttt taggatttat tttggaggaa gattttgtct ctattattta
      181 ttattttaaa tttatttatt ttgtataaga tctattcttt gaggcgggaa aaatcgaaat
      241 acttgattta tacggcctat atcatatttg gggtaaatgt agtatatggt attcaatggt
      301 tattaaaaga actgatttca actatttccc cttaatgtgt atggaaacta taacaaaacg
      361 cataagatta aggtttgtgt acctgttctt atacgttttt gtcatattga agattttcgg
      421 taagttttct acttttgcga agtctagttt atactcaatg aaaatcaaag agtaaactag
      481 ccgtaggttg ctcaaagtac agctttgagg ttgcagataa aactgacgaa gtcagctcaa
      541 aacatggttt tgaggttgta gatgaaactg acgaagtcag ctcaaaacat ggttttgagg
      601 ttgtagatga aactgacgaa gtcagtaacc atacgtacgg caaggcgacg ttgacgtggt
      661 ttgaagagta ttagaatgct ttcccaacca aaatctctgc ttcgatggta atctctgtca
      721 gtttgctcca gtcaattttg ctctggtcaa cgtgaaagag gagaggagtc atactgagta
      781 gggcttggag ttgttctgct gtgatagtct tagtcagaga ggcagtttga ctagatagga
      841 tggtaaagtg ttcctggaaa tgctccttga tatcttggtt agaatactcc ttgtttgtca
      901 gctggtcctg tactctttga cggatttctt tgaggtgatt ttcagttggg ataaccttta
      961 tcaagatacc gtctttggat aaaacgcgac gaaattctcc atagttggca ggtgagaaga
     1021 tatcaagcag aatatccatg ttagcgtctt ttataggaag tcgtgccaag tcgccaacga
     1081 accaattgac tgcccagttg ggttcacttt tagcagcgat ttggactgaa tctttggaga
     1141 tgtcaaaggc atagaaagtt ttttcagagt gactttcttg tagtttgcga gaatagaatc
     1201 cttcaccaca accgatatcc aaaattgttg tggtagtttt tgagcttgca agcaagtcag
     1261 atacagcatc taagatagct tggtaaaagc cggcttctag gatttgttga cggttttgaa
     1321 aattttcctt gtcgtagtta gcagattgct tgatttgagg gactagattg acatagccaa
     1381 atttcgccaa gtcaaaagaa tgacggttgc agcacttgaa attagtctct aacagagtca
     1441 gattttcttg acagatagga caggcaaagg cagtcgcaga agcaaaacgc tgaagtttgg
     1501 gcttgagatt tgtattcata gtttaagtgt atcaaaagag gcaaatgaaa gcaactttag
     1561 gaataagtag atgggagatt cagtagaaat aaaactaatt ctccaattta tagaatgaaa
     1621 ttgcttttat atctaaattg ctatataata atgataagga ggaaataagt atgttaaaag
     1681 aagtattaac cgttgcaaaa gttgcgaaaa aatcttcatt atttttgggt ggtgtcgcat
     1741 ttggtaccct tggtttgaaa atcttagcaa gtaaggaagc taaaaaaggt tattctaaag
     1801 ctttggctaa ggcttacaag ttgaaagacg agctagatgc atctgtttct gttgtgaagc
     1861 aacatggaga cgatgtcttg caagatgcca aatatttgta cgagcaagag aaaaaagaag
     1921 agcaattaga tagccttata ggtgaataat atgtctttta aagtgctaca tagaggatac
     1981 caacatatcc gactatcatc ttctttttca cttaccttgg atattcaaga ctatcttcgt
     2041 tccttggcga gagatgaaaa ggggattgag tctatccagt tttacatgga tcaacagcac
     2101 tttactctac gcataaaaga aggcttttct gtattagata atgcagaagc ctttttaaaa
     2161 agaattgata aagggaaagt ttctgagttg atgactcttc ccattcgtag agaagagagt
     2221 gcttattcta ttgtttcagg tgcagcggtt aagcgtgtac tttttcgtag ttttgtgccg
     2281 tatcctattc gctatatatg gacttgttat caggctttgg gttatattag agaagcctat
     2341 caaacactag cgcgtaagga actaacgatg gaagtcttgg actgttcggc tattttattg
     2401 tctttgttta tgaaccaatc caagacagct agcaatatca tgtttatgct tgatttgggg
     2461 aatcatttag atcagtggtc cttgaaaaaa actgcaacag atttagaaca gagtcttctt
     2521 gcaaaagaga gcgatgtatt cctagtacag ggcgatacgg ttgttagtat caagagttcc
     2581 gatgttcaaa taggagatgt cttgatctta tctcaaggaa atgaaattct gtttgatgga
     2641 caagtagttt caggtttagg tatggtcaac gaaagttcct tgacaggaga gagttttcca
     2701 gttgaaaaaa gagagtctga tttggtttgt gcaaatacag tattagaaac tggagagtta
     2761 cgcattcgtg taacagataa tcagatgaac agccgtattt tacagctgat tgagttgatg
     2821 aagaaatctg aagaaaacaa gaaaacgaaa caacgctatt tcatcaagat ggcggataaa
     2881 gtcgtcaaat ataatttctt gggggctggg ctgacttacc tattgacagg ttctttttct
     2941 aaggctattt ctttcctatt ggtcgatttc tcctgcgctt tgaaaatctc tactcctgta
     3001 gcttatttga cagttatcaa ggaagggttg aaccgtgaaa tggtgattaa ggatggagat
     3061 gttctggaga aatatctgga agttgatact ttcttgtttg ataagacagg aacaatcaca
     3121 actagttatc ctatagttga aaaggtgtta ccttttggag actatagtga ggaagatatt
     3181 ctcagaatca gtgcctgtct tgaggaacac atttatcatc ctattgctaa tgccatcgtc
     3241 aagcaagctg agatagaggg aattgaacat gaggaaatgc atgggaaact ccaatatatc
     3301 gcaagcaagg ggatcaaatc tcatatagat ggccaaccag ttcttattgg gaattatgtc
     3361 ttgatgcagg atgagcagat tcatatcagt tcagaacaaa atgctttaat tgaagagtac
     3421 aagagtcact acaatctctt attcttggct tatcaaaatg aattgattgg aatgttctgc
     3481 attcatactc ctttgagaaa agaagcaaaa acagccttgg ataaacttaa ggcacagggg
     3541 aaaaaattga ttctggcaac aggagacacc ttgattagga cagaggaatt agtcaaagat
     3601 ttgccctttg atcaggtcta tacagacttg aaacctgatg ggaaatttga gttagtagag
     3661 aaactgcaga aagcaggtca cactattttg atggttggag atggattgaa tgactcagcg
     3721 gctttaactc tatcagatat cggtgtggtg atgaatgaga gtgcagatat ttctaagcag
     3781 atgagtgata tcttattgtt agataatcgt ttggatttct tccaagagtt ggattcgtta
     3841 tcatcatctt tgcaaacact catcaagaaa aatattcagg ataccgttgt cgtaaatagt
     3901 agtttgattg gctttggctt atttaattgg ttcagtcctt caaatctctc tatcttacat
     3961 aatctaacaa ccttgcgcat tgtcctgcgt agcctgtcta ttaaaaatag atagatgaga
     4021 gttattaaca gcaaaaagga gttacctttc tcgaggttaa ctcctttgtt tatagataaa
     4081 tgttgaatag tttagtaagt caatacaaag tattttttct tcccacgacg gataacagtc
     4141 agttcattct ctaacttatc agcgtcactc aagacatagt caagctcttg gatgcggtcg
     4201 ccgtttacgt agatggctcc gttttggacg tcttcacggg cttggcgttt tgagttaact
     4261 ataccagatg agacgagcag ttccacgata ttgttgtttt cgtctgcctg tacttggtag
     4321 ttgggcacac cacgaagtcc ttgtttgagc tctttgacag aaaggttttt gatgtttcct
     4381 gcaaagagtt gctcagtgat gttaagtgct tctttgtagg cttcttctcc gtgaacaagt
     4441 gtaacaactt cacgagccaa gactttttga gccaagcgtt cgtgtggcgc tgcttcaaat
     4501 tgtttacgaa tatcttcaat ctcatcaagt gacaagaaag taaagatttt caagaagcga
     4561 acagcgtcag cgtccatcac gttcatccag aattggtaca tttcgtatgg agaagtcttt
     4621 tcgggattga gccagacggc atttccttct gatttaccaa atttcttacc agttgcatct
     4681 gtgattagtg gaacagtgat aacgtgacca gtcttgtccg ccttacgacg aagcaattcg
     4741 gtaccagctg tcatatttcc ccactggtca gaaccaccga tttgaagagt gacattatgg
     4801 tcttggttaa ggacgaagaa gtcataccct tgcatgattt ggtaagcgaa ctcagtgtaa
     4861 gaaattcctg tttcgatccg ttttttaaca gattccttac tcatcatgta gttgaccgtg
     4921 aagtattttc caatatcacg gaggaagtca atgaagctga tgctgccaaa ccagtcgtag
     4981 ttgttgacca tgacagcctt gttttcgcca ttttcaaagt caagaaaacg agaaagttgt
     5041 ccttggatag acttgaccca gccatctact gtgtcttttg tttggagact acgttcagca
     5101 tctttgaagg acggatctcc gatgagacct gtagcaccgc caacgagcgc ataaggtttg
     5161 tgacctgcta gttgcaagcg acgacttgtc aagattgcga caaggtggcc taggtgaagg
     5221 ctgtcagcag ttggatcgta gccagtataa taagaaactt gaccttcttc tagggcttta
     5281 cgcaaagctt cttcatcagt cgtttgaaat atcaaaccac gctcttttag ctcatcaaaa
     5341 atgtgcatgt gttttttctc ctttttaaaa ttattgtttc tacctattgt atcacaaact
     5401 tgggcaatag cctagcagaa tagggaagaa agtggctatt ttattgaaaa tttccctttt
     5461 gtggtataat agataaagtg aggaaatcca tgcaaaatca attaaatgaa ttaaaacgaa
     5521 aaatgctgga atttttccag caaaaacaaa aaaataaaaa atcagctaga cctggcaaga
     5581 aaggttcaag taccaaaaaa tctaagacct tagataagtc agtcattttc ccagctattt
     5641 tactgagtat aaaagcctta tttaacttac tctttgtact cggttttcta ggaggaatgt
     5701 tgggagctgg gattgctttg ggatacggag tggccttatt tgacaaggtt cgggtgcctc
     5761 agacagaaga attggtgaat caggtcaagg acatctcttc tatttcagag attacctatt
     5821 cggacgggac ggtgattgct tccatagaga gtgatttgtt gcgcacttct atctcatctg
     5881 agcaaatttc ggaaaatctg aagaaggcta tcattgcgac agaagatgaa cactttaaag
     5941 aacataaggg tgtagtaccc aaggcggtga ttcgtgcgac cttggggaaa tttgtaggtt
     6001 tgggttcctc tagtgggggt tcaaccttga cccagcaact aattaaacag caggtggttg
     6061 gggatgcgcc gaccttggct cgtaaggcgg cagagattgt ggatgctctt gccttggaac
     6121 gcgccatgaa taaagatgag attttaacga cctatctcaa tgtggctccc tttggccgaa
     6181 ataataaggg acagaatatt gcaggggctc ggcaagcagc tgagggaatt ttcggtgtag
     6241 atgccagtca gttgactgtt cctcaagcag catttttagc aggacttcca cagagtccca
     6301 ttacttactc tccttatgaa aatattgggg agttgaagag tgatgaagac ctagaaattg
     6361 gcttaagacg ggctaaggca gttctttaca gtatgtatcg tacaggtgca ttaagcaaag
     6421 acgagtattc tcagtacaag gattatgacc ttaaacagga ctttttacca tcgggcacgg
     6481 ttacaggaat ttcacgagac tatttatact ttacaacttt ggcagaagct caagaacgta
     6541 tgtatgacta tctagctcag agagacaatg tctccgctaa ggagttgaaa aatgaggcaa
     6601 ctcagaagtt ttatcgagat ttggcagcca aggaaattga aaatggtggt tataagatta
     6661 ctactaccat agatcagaaa attcattctg ccatgcaaag tgcggttgct gattatggct
     6721 atcttttaga cgatggaaca ggtcgtgtag aagtagggaa tgtcttgatg gataaccaaa
     6781 caggtgctat tctaggcttt gtaggtggtc gtaattatca agaaaatcaa aataatcatg
     6841 cctttgatac caaacgttcg ccagcttcta ctaccaagcc cttgctggcc tacggtattg
     6901 ctattgacca gggcttgatg ggaagtgaaa cgattctatc taactatcca acaaactttg
     6961 ctaatggcaa tccgattatg tatgctaata gcaagggaac aggaatgatg accttgggag
     7021 aagctctgaa ctattcatgg aatatccctg cttactggac ctatcgtatg ctccgtgaaa
     7081 agggtgttga tgtcaagggt tatatggaaa agatgggtta cgagattcct gagtacggta
     7141 ttgagagctt gccaatgggt ggtggtattg aagtcacagt tgcccagcat accaatggct
     7201 atcagacctt agctaataat ggagtttatc atcagaagca tgtgatttca aagattgaag
     7261 cagcagatgg tagagtggtg tatgagtatc aggataaacc ggttcaagtc tattcaaaag
     7321 ctactgcgac gattatgcag ggattgctac gagaagttct atcctctcgt gtgacaacaa
     7381 ccttcaagtc taacctgact tctttaaatc ctactctggc taatgcagat tggattggga
     7441 agactggtac aaccaaccaa gacgaaaata tgtggctcat gctttcgaca cctagattaa
     7501 ccctaggtgg ctggattggg catgatgata atcattcatt gtcacgtaga gcaggttatt
     7561 ctaataactc taattacatg gctcatctgg taaatgcgat tcagcaagct tccccaagca
     7621 tttgggggaa cgagcgcttt gctttagatc ctagtgtagt gaaatcggaa gtcttgaaat
     7681 caacaggtca aaaaccagag aaggtttctg ttgaaggaaa agaagtagag gtcacaggtt
     7741 cgactgttac cagctattgg gctaataagt caggagcgcc agcgacaagt tatcgctttg
     7801 ctattggcgg aagtgatgcg gattatcaga atgcttggtc tagtattgtg gggagtctac
     7861 caactccatc cagctccagc agttcaagta gtagttctag cgatagcagt aactcaagta
     7921 ctacacgacc ttcttcttca agggcgagac gataattctc taaatgaagt ggccaatcaa
     7981 tggattgcca cttttttcct ttcgtgttac aataagggta tgaatcagta tcagaaaaag
     8041 attgttaacg gaaaaattta ttcgctccta tccggcttaa tatggggaat ctgtggaatt
     8101 ttaggagagt acttctttac tcattatcag gtgtcttcgg gctggattac ctctatgcgt
     8161 ttgacactgg cagggagtct tgtactcatt tggtctgcaa tacaattaaa atcgcaagtg
     8221 ctagatattt ggcgagacaa gaaaaattac ctgccctttt tagcctatgc tattttgggg
     8281 attttttcag ttcagtattt tttctatctc tgtgtagaat actcaaatgc tacgacagca
     8341 actattttac agtttattag ccctgtcttt atcctctttt acaatcgctt ggtttatcaa
     8401 aaacgagcgt caaaaagcgc tgttttctat gttttggttg ccatgctggg tgtttgcttg
     8461 atggcgacaa agggagacct ctctcagtta tccatgacgc cgctagctct tataacaggt
     8521 ttgctgagtg ccatgggtgt tatgtttaat gttatcttgc cccaaccttt tgctaagcgt
     8581 tatggttttg ttcctacggt tgggtggggg atgattttgg caggtttgtt tagcaatgtc
     8641 ctctcgccgg tttatcagct ttcctttact cttgatattt ggagtatctt gatttgcctc
     8701 attatcgctt tctttggaac ggcttttgct tttttcattt ccatgaaggc tgtgtccttg
     8761 gtttctcctt tggtggttgc cgttatcagt gccagtgaac ctctctcttc tgctctcttg
     8821 agtgttttgt tcttaggatt agtagtggat tggtccctcc ttctagctat agccttgatt
     8881 attttaccca tgatattttt gtctatagaa gaagcgaaag aaagtagata aaaagtcttt
     8941 tcttaattct aaaagtgtgc tatactggaa tagtcaataa aacacgggga gatagttgtg
     9001 aagagtgttt ttaggttgta tcggtagggg cttgctttta ccgacagcac gttttatctc
     9061 acatccctaa aaaatcatac ctaaaaacag acaaaaaggt ttcaaatttg aggccttttt
     9121 tggtagaata ggtatcatta taacgaacca ggaggcacct atgactgcta caaaaatgaa
     9181 cgctcaagaa attatccaat ttatcgccaa tgctgaaaag aaaaccagtg tcaaagtaac
     9241 ctttgagggg caactcgcaa ctgctgtgcc tagctctgtt gtcaaactag gaaatgtcct
     9301 attcggagac tggaaggatg tggctccgct tcttgaaggt ttggtagaaa atcaagatta
     9361 tgttgtcgag caagatgctc gtaattctgc agttcctttg ctagataagc gtgctatcaa
     9421 cgctcgtatc gagccaggtg cgattatccg tgaccaggtg gaaattggtg acaatgctgt
     9481 tatcatgatg ggatctgtta tcaatatcgg tgctgaaatc ggtgctggaa ccatgattga
     9541 catgggtgcc atccttggtg gccgtgccat cgttggaaaa aatagccacg ttggtgcagg
     9601 tgcagttttg gcaggtgtga ttgagccagc tagtgctgaa ccagtccgtg tcggagacaa
     9661 tgttcttatc ggtgctaatg cagtggttat cgaaggagtc caaatcggta gtggttcagt
     9721 tgtcgcagca ggagctattg ttacccaaga tgtcccagaa aacgtggtag tagcaggtgt
     9781 tccagctcgt attatcaaag aaattgatgc ccaaactcaa caaaaaacag cgctagagga
     9841 tgcgcttcgt accttgtaat tgtaaaagta aaaaagaggc ggaacccttt ttccagcctc
     9901 tttctgctat atcggaggac agatagatgt tagatttgat tcagactaga cgagatttac
     9961 accagattcc agagattggc ttggaggagt tcaagactca ggcttatttg ctggatgtga
    10021 ttgagaaatt gactacgggc aaggattttg ttcaaattcg tacttggcgg acagggattt
    10081 tggtctacct gcagggaagt cagccggagc gaaccattgg ttggcggaca gatattgatg
    10141 gcctgcctat cgtcgaacaa acaggtctac cttttgcttc tcaacaccaa ggtcgcatgc
    10201 atgcctgtgg ccatgatttt catatgacca ttgccttagg ctgtcttgag cgcgcccttg
    10261 aggagcaacc caagaataat ctgctctttc tatttcagcc tgctgaagaa aatgaagctg
    10321 gtggtatgct catgtatgag gacggtgctt ttggagattg gttgccagat caattctatg
    10381 gtctccatgt tcgtccagat ttgaaagtcg gccagattgc gactaatact catacactct
    10441 ttgcaggaac ttgcgaggtg aagatccgtt tcaaaggcaa aggtggccac gcagcttttc
    10501 ctcatgaagc caatgacgcc ttggtggcgg ctagttactt tgtgacccaa gtgcagtcag
    10561 ttgttagccg caatgtcaac ccaatcgagg gagcagtggt gaccttcggc gtttttcaag
    10621 ctggaaccac caacaatgtc atcacagaca cagctttttt acatggaact attcgtgcct
    10681 tgacacagga catgagtctc ttggtgcaaa agagggtaaa aacagttgca gaaggggttg
    10741 cagctgcctt tgatatggaa gtcgaagtgg aactcaaaca aggtggatac ctacctgttg
    10801 agaacaatcc agccttggcg cgtgaactga tggacttctt tgacgaaaaa gacggaatcg
    10861 agttgattga tatcgaacct gctatgacag gcgaggactt tggttatctc ctttcaaagg
    10921 ttgatggcgt gatgttttgg ctaggtatcg atagtcccta cgctcttcat caccctcaaa
    10981 tgagtcctaa ggaagaagtc ttagctattg gggtggctgc ggtgtctagt ttcctgaaaa
    11041 agaaggcagc agaatagagg gcttgtctat gaaatcggaa ttacgcaagc aagtcttgca
    11101 tgaaatgaag gctatatctc aagagcaaaa acaggctata gaccaagctt taaccgagcg
    11161 gcttttacaa cacccctttt atcaagaagc caaggtcatc gcaacctacc tctctttttc
    11221 tcatgagttt caaacgcagg aactgattga gcaggcgctg aaggacggca agaaggtttt
    11281 aatacccaaa acttatccca aggggcgcat ggactttgtg gtctatgatc cgcaacagtt
    11341 ggtaaaaact ttctttgcct tactggagct acagggagat ttggaagtgg tggatgcatc
    11401 tcagattgat ttgattcatg ttcctggtct ggcttttacg acgaaaggat atcggatagg
    11461 atacggtgga ggctattatg accgctatct ggaacatttt tctggtcgta ctttgagtac
    11521 gatatatcct tgtcaaattc aggactttat acctgaaaac catgatattc ctgttcagga
    11581 ggtattaatt gatgaaggaa atctttgata gacgttaccc tgtgacgagt ttcttcctct
    11641 tagtgacggc cttggtattt ttactaatgt tggtcactgc aggcggaaac tttgacaggg
    11701 cagatacatt atttcgattt ggagccatgt atgggccagc tattcgcctc tttcccgagc
    11761 aggtttggcg tctcttgtct gccatttttg ttcatattgg gtgggaacat ttcattgtta
    11821 atatgctttc actttattat cttggaaggc aggtagagga gattttcggt tctaagcagt
    11881 ttttctttct ctatctttta tcaggaatga tgggcaatct ctttgttttt gtatttagtc
    11941 ctaaatcctt agcagcaggc gcctctacct ctctttatgg gctatttgcc gcgattattg
    12001 ttcttcgcta tgcaactcgc aatccttata tccaacagct agggcaatcc tatctgacac
    12061 tttttgtggt taacattatt ggaagtgttc tgattccagg aatcagccta gcaggccata
    12121 tcggtggtgc agttggtggc gcatttctag cagttatctt tccagttaga ggagaaaaac
    12181 ggatgtataa caccagccag agattaggag cggtagtctt gttcgtagga ctcgccattt
    12241 tgcttttcta caagggaatg ggattgtgac gactaatact caatgaaaat caaagagcaa
    12301 actaggaagc tagccgcagg ttgctcaaag cactgctttg aggttgtaga tagaactgac
    12361 gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt cagtaaccat
    12421 acctacggta aggcgatgct gacgtggttt gaatttgatt ttcattgagt ataatatgta
    12481 aacttgaata agaaaaaagc tacttaaaaa atgagtagct tttaatgtgt tttggaacca
    12541 ttcgaaacaa cacagctcta aaatagttat tagcgttgac taggttctgc aatgccgcca
    12601 ccaaagttcc caaggtaatt tccatcgtca tcatacacat ctccattatt atgatctttg
    12661 tagtagtatc tttgatagcc gtcagcacca gtgcctttat atctgtagac attatttgct
    12721 ccgctgtttg aattgctaga tgaatattgt tctgatgatc caccatttgt tgaactatct
    12781 cctgaaccag aataatcttg accgccataa ttatttgaat atccattagt ttgttcagat
    12841 gaactataat ctacatttga attggaagat ccattataag actcattaga agcactacca
    12901 gaagaattac tttgactaga agattccttt gctttttcag cagctttttc agcagctttt
    12961 tcttcttctt tcttcaaaag ttcgatttgt ttttgatatt tatcaacaag atcatttaga
    13021 gatttaagag tttcttccaa attctcttgt ttaaaagttt catcaacttt tttgataaaa
    13081 tcaatttgtt ttgtaatttc ttttacattt gtttcaagag ttgctttatc tttaaatttg
    13141 tcatctgaat ctttaacatc ataagccaaa cctttttgca aatgaaaatc tacaattgct
    13201 ttagatgatt ctttgacttt gttattaaga tagtctttac ctgcatcggt tttaagtgaa
    13261 gtttcagata gttcttttaa tttagaaata gattcatctg aagatgcatc aaatgattca
    13321 aatagtttat caacttgttc tttgtctgat ttgctatctt tttctaattg tacaattttt
    13381 gcttgttgtt ctgcttgttg ttggttatat gaataccatc caaaccctaa taccaataca
    13441 acaccaactg caactgatga aatgattact tttttattgt tttcgataaa ttctacaaat
    13501 ttgttctcca taatcttttt ctttgatagt gtccgcaatt actttaaaat taaaattaca
    13561 cctatctttc tcccttatta tatttttatt aatagcttcc acaaaattaa acaacttatg
    13621 ttcggctcgg ttgattta
//
LOCUS       DAVHEW010000050        13196 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVHEW010000050 DAVHEW010000000
VERSION     DAVHEW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.050"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..666
                     /locus_tag="VVL53_001336"
                     /pseudo
     CDS             1..666
                     /locus_tag="VVL53_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(752..1954)
                     /gene="rpsA"
                     /locus_tag="VVL53_001337"
     CDS             complement(752..1954)
                     /gene="rpsA"
                     /locus_tag="VVL53_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU4183504.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2116..2187)
                     /locus_tag="VVL53_001338"
     tRNA            complement(2116..2187)
                     /locus_tag="VVL53_001338"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2153..2155),aa:Gln,seq:ttg)
     gene            complement(2197..2277)
                     /locus_tag="VVL53_001339"
     tRNA            complement(2197..2277)
                     /locus_tag="VVL53_001339"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2241..2243),aa:Tyr,seq:gta)
     gene            complement(2327..2557)
                     /locus_tag="VVL53_001340"
     CDS             complement(2327..2557)
                     /locus_tag="VVL53_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU4183505.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2725..3369)
                     /gene="pcp"
                     /locus_tag="VVL53_001341"
     CDS             complement(2725..3369)
                     /gene="pcp"
                     /locus_tag="VVL53_001341"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU4183506.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3384..4307)
                     /locus_tag="VVL53_001342"
     CDS             complement(3384..4307)
                     /locus_tag="VVL53_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU4183507.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4304..4993)
                     /locus_tag="VVL53_001343"
     CDS             complement(4304..4993)
                     /locus_tag="VVL53_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU4183508.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5058..5762)
                     /locus_tag="VVL53_001344"
     CDS             complement(5058..5762)
                     /locus_tag="VVL53_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4183509.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASHLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(5818..6840)
                     /locus_tag="VVL53_001345"
     CDS             complement(5818..6840)
                     /locus_tag="VVL53_001345"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU4183510.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6970..9441)
                     /gene="parC"
                     /locus_tag="VVL53_001346"
     CDS             complement(6970..9441)
                     /gene="parC"
                     /locus_tag="VVL53_001346"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU4183511.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9861..11804)
                     /gene="parE"
                     /locus_tag="VVL53_001347"
     CDS             complement(9861..11804)
                     /gene="parE"
                     /locus_tag="VVL53_001347"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU4183512.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSNWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGFKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAVVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11940..12581
                     /gene="plsY"
                     /locus_tag="VVL53_001348"
     CDS             11940..12581
                     /gene="plsY"
                     /locus_tag="VVL53_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU4183513.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12624..>13196)
                     /locus_tag="VVL53_001349"
     CDS             complement(12624..>13196)
                     /locus_tag="VVL53_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4183514.1"
                     /translation="KRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPAS
                     TQKLYLSPVLDGFNSEIIAFNLSCSPNLEQVQTMLEQAFKEKHYENTILHSDQGWQYQ
                     HDSYHRFLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYGYEKTFKSLNQLEQAI
                     IDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT         3816 a         2967 c         2306 g         4107 t
ORIGIN      
        1 cgatatcatc actgttcttg aaggtagaac acaagctatc atccgagatc actttcttaa
       61 atatgataga gccgtccgat gtcgcgtcaa aatcattact atggatatgt ttagccctta
      121 ctatgactta gctagacaac ttcgctttca aatttctaga tcatgaatca gtttcatcga
      181 aagtctcatg aatacaaggc tatcaagcgc tactggaagc tcatacaaca ggatagtcgt
      241 aaactgagtg ataagcgatt ttatcgtcct acttttcgta tgcacttaac caataaagag
      301 attttaaaca agcttttgag ttattcggaa gacttgaaac accactatca gctctatcaa
      361 ctcttgctgt ttcactttca gaataaggaa ccggagaaat ttttcggact tattgaggac
      421 aatcttaagc aggttcatcc tatttttcag actgtcttta aaaccttcct caaagataaa
      481 gaaaagcttg tcaacaccct tcaactacac tattctaacg ccaaactgga agcgaccaat
      541 aatctcatca aacttatcaa gcgcaatgcc tttggttttc ggaactttga aaacttcaaa
      601 aaacggattt ttatcgctct gaatatcaaa aaagaaagga caaaatttgt cctttctcga
      661 gcttagcttt tcttcaaccc actacagttg acaaagagcc agaaaaatcc cttgtttagt
      721 aaacaaagga ttttgtgaat tttcaatttg attaaagttc gatatcacca aacaaatcag
      781 ccattgaaaa tcctgtttgt gtttctggaa gttcgaaatc acgcttttct tgacgtcttg
      841 gacgacgtgg acgagcagca cgtttttctt ctttttgtcc ttcttcttgg gctggacgtt
      901 cttcaagagc tttaatagaa agtgacacgc gttctgcatc tgcgttaact tcaagaactt
      961 taacttgaac ttcttgacca actttaagag cttcttttgg attttcaatc cgtttgtgtg
     1021 aaatttgtga tacgtgaaca agtccatcga tacctggcaa tacttcaaca aatgcaccga
     1081 agtcagtcaa acgtttaact gttccttcta ctacatcacc tttagccaat ttttgctcaa
     1141 cgccatccca tggtcctggt actgttgctt taagtgaaag tgatacacgt ccttcttctt
     1201 cgttaagatc aaggattttc acttcaattt cttcaccaac agttacaact gattttggtg
     1261 atacattacg ttcatgtgac aattcagtca agtgaaccaa tccgtcaaca ccaccaaggt
     1321 cgacgaaagc gccgaagctt gtgatacgag caactttacc agttacaaca tcaccaacag
     1381 ccaatttacc gaatacttca gcgcgagctg ctgcagtagc tgcttcaaca acttcacgac
     1441 gtgaaaggat gaagcggttt tctttagcgt taacttcttt gattttagta tcaaattctt
     1501 gacctacaaa acgctcagcg ttacgtacga aacgagtatc caacattgaa gctgggataa
     1561 atccacgaac accttcaaat tctactgaaa gtccaccttt aacggcacgc gttcctttaa
     1621 cagtaacaac ttcttcttcg cgaccaacaa gtttgtccca tgctttgcga gcttcaaggc
     1681 gttttttaga tacaaggtat gtaactgtat cagtatcttt accaactact tgacgaagta
     1741 caagaacatc caatacttct cctactttaa caaagtcatt gatatctgca tcacgatcgt
     1801 ttgtcaattc gcgaagagtc aagacacctt caacaccagt tccagagatt gcaacgttag
     1861 cttgagtcgc atcaactgtc aatacttcag cactaacaac atcaccagtc tcaacttgac
     1921 taacgctatt tagcaaatct tcaaattcgt tcatctaaaa aatcctccaa caatcaagtt
     1981 tttcttaaac ttgacatact tataattttt ttcctaagca cccgcaagga catgttcata
     2041 tcattcacgt ctttcaatta taaaaataaa ataccctaag atattttgct ttctatcttg
     2101 attattacct aagaactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
     2161 cttgccttac cgcttggcta taccccatta atgaaatgga gagagaggga ttcgaacccc
     2221 cgaacccgaa ggagcggatt tacagtccgc cgcgtttagc ctcttcgcta tctctcctac
     2281 aatcaacatg gactattata tcatgaaaat ttcaaaaaaa caagacttat tttgctaaat
     2341 tataattttc aatcaaaatt tccacagctt tttccaattt tttataaaaa ctatcgacat
     2401 tcccattctt tataatggca aattgtccgt ctaattcggc aatttctccg ataacttttt
     2461 taccgatagt caatgtataa ccttcaaaac tgtctttacc aacattaact ttggcatccg
     2521 ctacttgaat ttcgattttt ttatctttct tgctcatttc atacctctct tcactttttc
     2581 tattttacaa aaaaatccta aaactagcaa gaaaaaactc tatatcaggc ttctctgata
     2641 tagagtttta tgtaaacaac ctcaattgat ttagcatagt taatagttag taaacaagca
     2701 taattgacta tcctttttag gttatcagtg agtctcaccg cctactaact gcaaatcctt
     2761 atctttgtag tctacaatag cttcaatagc tacctctatg cctcttacaa tatcacatag
     2821 actcattcct gcagtattcg gcttatttac tacttgttcc atcatatatg gaatatgcat
     2881 gaaacctgct ctcatattag ggaatttttt atccactaaa taaagagctt ggtacatcaa
     2941 atgattgcaa acaaaggttc ctgcactatt ggatacaact gccggaagtc cctgtttttt
     3001 gatagcttgt accatcgctt tgataggtaa actactaaaa taggccgatg ctccatcaat
     3061 acgaatcggt gtatcaattg gttgattgcc ttcgttatca ggtatgcgag catcatcttg
     3121 attaatagcc actcgttcag gtgttaagcc ggtcctgccg cctgcttgtc caatacaaag
     3181 tacagcatct ggttgatatc gtaatatttc tgcctctaaa acttctgacg acttataaaa
     3241 aaccgttgga atttctaccc agcgaacttc agccccatta atctcagatg gtaataattt
     3301 tacagcctcc aaagctggat taatcttttc acctccaaaa ggattaaaac ctgtaaccaa
     3361 tattttcatt ttattttcct ttactaaaat gcgagaaagt acattaagaa tatgtgaata
     3421 acaatcatta ctagagcaac acctgcttga gcctttataa cgccattctg atctttcata
     3481 tccatcaatg ctgctggtag agcgttaaaa ttagcagcca ttggggtcaa taaggtccca
     3541 caataacctg ctgtcatggc aagagcacca gccacaattg gattagctcc cagagcaaat
     3601 acaaagggaa ctccaacacc tgctgtaata acggtgaatg ctgcaaaagc atttcccata
     3661 atcattgtga atagaaccat tccaagaaca taggccaaaa ctcctataaa gcgactatct
     3721 gaaggaacaa taccgctaat cagatgagag ataacatcac caacacctgc tacagtaaaa
     3781 atagccccca aagcccctaa taattgagga acaatcccac ttgttgaaac ttgctgagtc
     3841 attcgattat tttctgataa cagactctta gggtgactat tggtaatcac aagaacagaa
     3901 attgtagcaa acaaggcggc aaggctaatc gaaatcttgc taaattctgg aatcatttgc
     3961 gctaagacca acgcaagtat tgccatcagc ataactggaa taaaaatttt atttttcaac
     4021 ctgttagatt caatattggc tttcatttca tctaaggatg gcaaggttcc gatacggact
     4081 tgcttaaaca atgttaacag cgataatagg attacaataa taccaatact catatttggc
     4141 atataggaac cacctataaa cgtaatagac aatagagtcc aaaatgcaga tgtcccaagt
     4201 cgaactgggt ttgttttatc tttataacta caataggctg tatggagaaa ttgacaacca
     4261 atcacaatat aggtcaactc taatagttgc tttgccaact ctgtcatttt tgttctcctc
     4321 ccctagtctt ttttgatatc aattttttat caaataaata attataaatc cccactacaa
     4381 taagtgttat aacagcaaca ataatagatg tagaagcaat ccctgcataa ttgctttcat
     4441 agcctaactg atctaatgtt ccccctatca agaggactcc cccagcacct acaaacgtat
     4501 tttgagcaaa gaaatttcca aaattttcat tcgcagccgc acgcgctttt attgtctcat
     4561 cttcaacctc tgttaacttt ctacctaatt gagactctgc agctgcttct cccataggtt
     4621 gaaccaaagg tctgacaaac tgagggtgtc ctcctagacg aattgaaaag aaaccagcta
     4681 actctcgaat aaagaaataa actgtataga agtttccaac tgtcagacct ttaatctttc
     4741 gaatcaaatc gattgatcgt tgcttgagtc caaaggtttc tgacagcccc acaagaggca
     4801 aggtaaccat aaaaatcgtg agcactcgct gattgctaaa ttcttttccc aaaatctcca
     4861 aaaattcaac gagagaaaca cctgaaacta aagctgtaac caaaccagct aagactactg
     4921 ttgcaattgt atcaaatttt aaaataaaac ccacaacaat gattgctatt cctattaatc
     4981 taatccactc catatcaaac tcctttatat tcaaaatgac agtattttta aaattttatc
     5041 aagatcaata ccattcctta tttaatgtgt ttttctagtt ctttttggta tttgctattg
     5101 gattccaatt tttctttttg ccatttttta aaaacctcgt tatattcttt tgttgtaaca
     5161 atatcttttt gcaatttcat tcctttaaag atatatggat cccccttaat accaacttgt
     5221 gagtatggtt ttgagaatgg tactacgtta cttacaactg gagaaccacc agatgaagct
     5281 gttggcatca ataatgaact atctgtcgac caagcttgag ctttggcata tttttcatat
     5341 cttttctcta ggtcagtggt ctcagaaaca gcatcttcta acaatttctt atatttatcc
     5401 aaaccaggtt tagctacaac atccttatct tttcctttcg taataccaag gtgtttcatg
     5461 gcagaaccag attttggatc tataatattc aagtgagacg ctggatcttg atagcttgga
     5521 gcccatcctg tactgttcaa atcatagtct ttttgagaag gagcaacatt gccgtattta
     5581 tcattttcca tcaaaccatc aataacattt ccaataacgt ctgtcctcga tgttcgagtc
     5641 gctatactgt agcccaatga tgctggatct actgcataga cataagaaaa tgttgtcggt
     5701 gcatctgctt ctttatcagt ttttccacaa gccactaaaa tagctgacgt gctcaggacc
     5761 actcctgctg ttaagagcca ctttttctat ttcataaaga atctcctttg gtttatttta
     5821 atctactttt acaatccaac cttctggcgc ttcaatatcg ccaaactgaa tacccgtcaa
     5881 ttcattatat aatttacgcg tcacaggacc tacttctgtt tcactataga atacatggaa
     5941 atcatcacca tgttgaatac ctccaattgg agaaataacc gctgctgtac cacaggcacc
     6001 tgcctctaca aaacggtcaa gattatcaat tggaacatca ccctcaatag gagttaatcc
     6061 caagcgatgt tctgccaaat aaagcaagga atacttggta atagatggca agatagatgg
     6121 actcaatggt gttacaaatt cattatcagc tgtaattcca aagaagttag ctgatccgac
     6181 ttcttcaatc tttgtatgag ttgatgggtc cagatagata acatctgaga aatgacgtga
     6241 cttggccatt tttcctggta agagacttgc agcatagttt ccaccaacct tagccgcacc
     6301 tgtaccattt ggtgctgcac ggtcgtactc atcctgaatc aagaagttgg ttgggaccaa
     6361 accaccttta aagtaatttc caactggcat agcaaagatg gtgaaaatgt actcttctgc
     6421 cggttttacc ccgataatat ctccgacacc aatcaaaaga gggcgaagat ataaggttcc
     6481 acctgttccg tatggtggta cgtattcttc attcgcacgg acaactgctt tacaagcttc
     6541 tacaaacatg tctgtcggaa cttgtggcat caagagacgg tcacatgtac gttgcagacg
     6601 tttagcattt tcatcaggac ggaacagttg aacactgcca tccttagtac gataagcttt
     6661 caaaccttca aatgcttgtt gtccatagtg aagacttgga gaagactctg aaatatgcaa
     6721 agttgcatcc tctgtaagct ctccttgatc ccattgtcca tttttgaaat gagcaagata
     6781 gcgataaggt aatttcatat aggaaaaacc gaggttttcc caatcaatcg ttactgtcat
     6841 attccactcc ttattctaaa aacctatttc ttatattcta cactattttt ctaaaatagc
     6901 aagtatattt tgtaattttc agaaaattcc tccaataaaa accatcttag aactgattct
     6961 tcatttcact tatttatctt cagtaactac ttcctgaaga taagcgtcaa aaacttcttc
     7021 atctgaaatc gtgtcagaaa tgaagcttcc attgctagtg cgttctgaca agttcaagtc
     7081 ttgcaatcgg ctttcataga ttgttccttt attggattgg acaagcagag tttggtcgtt
     7141 cacatccact tccgtactga agaaatcgcc aacaaatcct tgctctgcaa ctgctcctgc
     7201 caagaagaca cgatgcggtt tgtttttcaa ctcacgcaag acttgtaatc ctcgtttggc
     7261 acggctggtt gctagaattt cctcaatgga aacacgtttc aagcttccac gctgggtcaa
     7321 gaggtagaag gacgaagtat tacagataaa gccagattgg aggacatcat cttctttcaa
     7381 attcatagcc ttgacacctg ctgccttagc accgacaacc ggaacctctt cgatattgaa
     7441 acgcagggca taaccatttt gactaaccaa gacaacatca tctagtttaa tcggagccac
     7501 tgctacaatc tgatctgtat cgtctttgag cttagcatac ttgacagact tagatctata
     7561 ggtccgccat ggagtgaatt cttttcgctc tacccgtttg atttgaccaa ggcgagtcgc
     7621 tgcaaagtag gttgtcgcat cgtcaaactg atccagtact tccacataaa ggatttcttc
     7681 attcgtttca aagtttgtga tggtttggct cagatgctct ccgatgtcct tccaacgaat
     7741 atctgccaac tcatggattg gtctgtagat gacatttcca agacttgtga acatcaagag
     7801 gtgctgggtt gtcttggcag attgaacaaa aatcaaacgg tcatcatcac gcttgccaat
     7861 ttcttccaag gtggaagccg caaaggaacg tggactggta cgcttgatgt aacctgcctt
     7921 ggtcacgctg acgtaggtat cttcctcagc gataagacta gctgtatcaa tctcaattgc
     7981 tttcgcagtg tcttctaaag aactcaaacg aggagttgca aatttcttct tgacctcacg
     8041 aagttctttc ttcatgagat tgtacatagt cctttcatca ccaataatag ccgccagcat
     8101 agcaatcttc tcacgaagct ctgcttcttc ttcctgcaag acaaccacat cggtattggt
     8161 caaacggtac agttgcaaag ttacgatagc ctcagcctgt tcttccgtaa aatcatagct
     8221 aactttgagg ttttccttgg cgtccgcctt attctcagaa gcacggataa gagcaatgac
     8281 ttcatccaaa atcgaaatca cacgaatcaa accttcgacg atatggagac gtttctcagc
     8341 cttttctttg tcaaagcgtg aacgcgccaa aatcacttct cgacggtgag cgatgtagct
     8401 agacaggatt ggaacaatcc caacctgacg aggtgtgaaa ttgtcaatcg ccaccatatt
     8461 aaagttgtag ttgatttgta ggtcggtgta cttaaataag tagttgagaa caagctcagt
     8521 attagcgtct ttcttaagtt cgatagcgat acgaagacca tcacggtcag actcatcacg
     8581 aacctcagca atcccagcta ccttgttatt aacacgaaca tcatcgattt tcttgactag
     8641 attggcctta ttgatttcat aaggaatctc agtaataacg atttgttcct taccaccttt
     8701 tagcttttca atttcagtct tggaacgaac aaccacgcgc cctttcccag tctcataagc
     8761 tttcttgatt tcatcacgac cctgaataat agcccctgta gggaagtctg gtccaggcaa
     8821 gaattccatg agtttatcaa tctttgcagt tgggtggtca atcatgtaaa ctgcagcatc
     8881 tatgacctca gctaaattat ggggaggaat gtctgtggca taaccagccg aaatcccagt
     8941 cgaaccattg accaagaggt ttggaaaggc tgctggcaag accgttggtt ctttctccgt
     9001 atcgtcaaag ttccatgcaa aaggaactgt ctttttctcg atatcctgaa gaaggtagcc
     9061 tgcaatttca gacaaacgtg cctcagtata acgcatagcc gcaggaggat ctccatccat
     9121 agaaccgtta ttaccgtgca tttcaactag aatctcacga tttttccagt tctgtgacat
     9181 acgaaccatg gcatcataga tagaagaatc cccgtgtggg tggaaattcc ccatgatgtt
     9241 cccgactgac ttggccgact tacggtagct cttgtcaaaa gtattgctat ccttattcat
     9301 agaataaaga atacggcgct gaaccggctt caacccatca cgaatatctg gcaaagcccg
     9361 gtcttgaata atgtacttgg agtagcgacc aaagcgctct cccatgatgt cctccaggga
     9421 catgttttga atgttagaca taagatacaa agcccataaa ataccaagtg aaaatagaaa
     9481 attcttgaag taagcaaact cacaagagaa tttatctttt tcacacagta tctagggcgt
     9541 gttcaactcc tttcaaagaa tgtagagtag gtttttatgc agtaaaagat attttacggg
     9601 aattcctccc gtgttcagtt acgataagta accaaactat cctgtttgta tttttcaata
     9661 tgaaaatctg gttttccaaa attagtctta gtttgtgtct taaccgctcc cttaagcgcc
     9721 tctttgagat aagcactcat agcagattct tcattaataa tcctgcaatt ttttcaaacc
     9781 aagattttca aactgctttt tcacatagtc attcacatcc gactctaatt tccagtttac
     9841 taacatatta ttttctttca ttaaaacact gtcgcttctt ctagcgtaaa cttgacatta
     9901 tcttcaatcc atttacggcg tggttctacc ttatctccca tgagaacatt gacgcggcgt
     9961 tcggcgcgcg ctaaatcttc aattgtgaca cggatgaggg tacgtgtttc tgggttcatg
    10021 gttgtttccc agagctggtc cgcattcatc tcaccaagtc ctttgtatcg ttggagggta
    10081 gcgcctttac cgaactgttt acggagttct tctagttctc cgtccgtcca agcgtaggcc
    10141 acttcttctt tcttgccttt acctttggac atcttataaa gaggtgggag ggcaatatag
    10201 acatgacctg cctcgactag cggacgcatg taacggtaga aaaatgtcaa gagcaaggtc
    10261 tggatatggg caccgtcggt atccgcatcg gtcatgataa tgatcttatc atagttggca
    10321 tcttcaatag agaagtctgc tccaacaccc gcaccaatgg tataaatcat ggtattaatc
    10381 tcttcatttt tgaggatatc cgccatcttg gccttggctg tattgacaac cttaccacga
    10441 agaggtagaa tagcctggaa cttgcggtca cgaccttgtt tggcagaacc accggcagag
    10501 tccccctcaa ctagatagag ttcattctta gcaggattct tagattgggc tggggtcaat
    10561 ttcccagaca acaagccctt atctttcttg tttttcttcc catttcggct ctcatcacgc
    10621 gccttacgtg ctgcttcacg agcatcacgg gccttgatag ccttgcggat gaggttagaa
    10681 gctaattccc cattttccat aaggaaaaag gtcaacttat cagccactat tccatccaca
    10741 actgggcgag ctagggggct tcctagttta tccttggtct gtccttcaaa ctgcaagtgt
    10801 tcttcaggaa ctaagataga aagaacgacc gctagtccct cacgatagtc tgaaccttca
    10861 aggtttttat ctttttcctt gagaagacct gttttacgtg catagtcatt catgaccttg
    10921 gtaatggcag acttgaatcc tgtctcgtgc gttccaccgt ccttggtgcg aacgttattg
    10981 acaaaggata gaatgttatc tgagaatccg tcattgtact ggagggctac ttccacttga
    11041 aaaccattgt cttccccttc aaagtaaaga actggcgtca agatttcctt atcttcgttg
    11101 agataagaaa caaaatcttg tactccattc tcatagtgga actcaatcgc ttcatctgtt
    11161 cgcttgtccg ttaaagacaa ggtcacattt ttcaagagaa aggctgattc attaaggcgc
    11221 tctgaaatgg tattgtactt gaaatctgtc gtagaaaaga tagtcgcgtc aggcataaaa
    11281 gtaactttgg tgcctgtttt agacttgggt gctgtaccga ttttcttcaa agtcgtgaca
    11341 ggttttccac cattttcgaa acgttgcttg taaactgcac catcacgggt aatttcaact
    11401 tctaaccagt tagaaagggc gttaacaacg gaagaaccca ctccgtgaag tccacctgat
    11461 gtcttatagc caccttgacc gaatttccct ccggcatgaa gaatggtaaa gataacctca
    11521 acagttggaa ttcccatagc gtgcatacct gtcggcatcc cacgtccatg gtcttgaacc
    11581 gttagactac cgtctttatt gatagttaca tcaatacgat caccaaaccc agacaaggct
    11641 tcatcgactg cattatcaac gatttcccaa actaggtgat gaagaccagc gccatcggtc
    11701 gatccaatat acatccctgg acgttttcgg accgcatcca acccttctag cacctgaata
    11761 gcatcatcat tataattgtt aatattgatt tccttttttg acacaaggaa cctcctattc
    11821 gttcatcttt actattctac aggttttcca aggattttgc aaaatttttc tttctccgat
    11881 gtgacaattt cagcagagat tctctgcttt tctttcccaa ttcatgatat aataggagta
    11941 tgattacaat agttttatta atcctagcct atctgctggg ttcgattcca tctggtctct
    12001 ggattggaca agtattcttt caaatcaatc tacgcgagca tggttctggt aacactggaa
    12061 cgaccaatac cttccgcatt ttaggtaaga aagctggtat ggcaaccttt gtgattgact
    12121 ttttcaaagg aaccctagca acgctgcttc cgattatttt tcatctacaa ggcgtttctc
    12181 ctctcatctt tggacttttg gctgttatcg gccatacctt ccctatcttt gcaggattta
    12241 aaggtggtaa ggctgtcgca accagtgctg gagtgatttt cggatttgcg cctatcttct
    12301 gtctctacct tgcgattatc ttctttggag ctctctatct tggcagtatg atttcactgt
    12361 ctagtgtcac agcatcgatt gcggctgtta tcggggttct gctctttcca ctttttggtt
    12421 ttatcctgag taactatgac tctctcttca tcgctattat cttagcactt gctagtttga
    12481 ttatcattcg tcataaggac aatatagctc gtatcaaaaa taaaactgaa aatttggtcc
    12541 cttggggatt gaacctaacc catcaagatc ctaaaaaata aaatgccagt tctgtactgc
    12601 ccccaaacag ttagacaaat aatttatcca aaggatttag ttctgtactg cacaggacta
    12661 agtcctttta gttttacctt aattcgtttg ttgttgtagt aatcaatata gtctataatg
    12721 gcttgttcca attgattaag tgatttaaat gttttctcat agccataaaa catttcggat
    12781 tttaaaatgc caaagaaaga ttccatccta ccgttgtctt ggctgttgcc cttacgtgac
    12841 atggatgctt gaattccctt actctctagg aaccgatgat aagaatcgtg ttgatattgc
    12901 cagccttggt cactatggag aatcgtattc tcgtagtgct tctctttgaa tgcctgttcc
    12961 aacattgttt gtacttgttc taaattaggc gaacaagaaa gattaaaagc aataatttca
    13021 ctgttaaagc catctaaaac tggtgataag taaagctttt gagtacttgc tggaatggca
    13081 aattctgtca catctgtgta gcacttttcc attgttttag agccttcaaa ttgtccttga
    13141 atgagattct ctgccttctt gccaacgtct cctttatgag aagaatattt tcgttt
//
LOCUS       DAVHEW010000051        13017 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVHEW010000051 DAVHEW010000000
VERSION     DAVHEW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.051"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            18..5588
                     /gene="zmpC"
                     /locus_tag="VVL53_001350"
     CDS             18..5588
                     /gene="zmpC"
                     /locus_tag="VVL53_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HEU4183515.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            5944..6798
                     /locus_tag="VVL53_001351"
     CDS             5944..6798
                     /locus_tag="VVL53_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4183516.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            6904..7461
                     /locus_tag="VVL53_001352"
     CDS             6904..7461
                     /locus_tag="VVL53_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEU4183517.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            7678..8442
                     /locus_tag="VVL53_001353"
     CDS             7678..8442
                     /locus_tag="VVL53_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU4183518.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            9009..10388
                     /locus_tag="VVL53_001354"
     CDS             9009..10388
                     /locus_tag="VVL53_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU4183519.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            10402..11067
                     /locus_tag="VVL53_001355"
     CDS             10402..11067
                     /locus_tag="VVL53_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU4183520.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            11202..11360
                     /locus_tag="VVL53_001356"
     CDS             11202..11360
                     /locus_tag="VVL53_001356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183521.1"
                     /translation="MKIKDQTRKLATGCLKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRC"
     gene            11422..11595
                     /locus_tag="VVL53_001357"
     CDS             11422..11595
                     /locus_tag="VVL53_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU4183522.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGWDQVGNFSKGQYIMMHS
                     LIYRTDLLRASQF"
     gene            11662..12072
                     /locus_tag="VVL53_001358"
     CDS             11662..12072
                     /locus_tag="VVL53_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU4183523.1"
                     /translation="MYYLPVDFYRYLIGREGQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            12188..>13017
                     /gene="pavB"
                     /locus_tag="VVL53_001359"
     CDS             12188..>13017
                     /gene="pavB"
                     /locus_tag="VVL53_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU4183524.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     A"
BASE COUNT         4160 a         2268 c         2908 g         3681 t
ORIGIN      
        1 gaaaaagcga ggttaatatg agccgaaaaa gcattggtga gaaacgccat agtttctcga
       61 tgagaaagtt gtcagtggga ttggtatcag ttactgtatc tagtttcttt ttgatgagtc
      121 aagggattca atcggtatcg gccgataata tggaaagtcc aattcattat aagtatatga
      181 ccgagggtaa attgacagac gaggaaaaat ccttgctggt agaggccctt ccacaactgg
      241 ctgaagaatc agatgatact tattacttgg tttatagatc tcaacagttt ttaccgaata
      301 caggttttaa cccaactgtt ggtactttcc tttttactgc aggattgagc ttgttagttt
      361 tattggtttc taaaagggaa aatggaaaga aacgacttgt tcattttctg ctgttgacta
      421 gcatgggagt tcaattgttg ccggccagtg cttttgggtt gaccagccag attttatctg
      481 cctataatag tcagctttct atcggagtcg gggaacattt accagagcct ctgaaaatcg
      541 aaggttatca atatattggt tatatcaaaa ctaagaaaca ggataataca gagctttcaa
      601 ggacagttga tgggaaatac tctgctcaaa gagatagtca accaaactct acaaaaacat
      661 cagatgtagt tcattcagct gatttagaat ggaaccaagg acaggggaag gttagtttac
      721 aaggtgaagc atcaggggat gatggacttt cagaaaaatc ttctatagca gcagacaatc
      781 tatcttctaa tgattcattc gcaagtcaag ttgagcagaa tccggatcac aaaggagaat
      841 ctgtagttcg accaacagtg ccagaacaag gaaatcctgt gtctgctaca acggtgcaga
      901 gtgcggaaga ggaagtattg gcgacgacaa atgatcgacc agagtataaa cttccattgg
      961 aaaccaaagg cacgcaagaa cccggtcatg agggtgaagc cgcagtccgt gaagacttac
     1021 cagtctacac taagccacta gaaaccaaag gtacacaagg acccggacat gaaggtgaag
     1081 ctgcagttcg cgaggaagaa ccagcttaca cagaaccgtt agcaacgaaa ggcacgcaag
     1141 agccaggtca tgagggcaaa gctacagtcc gcgaagagac tctagagtac acggaaccgg
     1201 tagcgacaaa aggcacacaa gaacccgaac atgagggcga agcggcagta gaagaagaac
     1261 ttccggcttt agaggtcact acacgaaata gaacggaaat ccagaatatt ccttatacaa
     1321 cagaagaaat tcaggatcca acacttctga aaaatcgtcg taagattgaa cgacaagggc
     1381 aagcagggac acgtacaatt caatatgaag actacatcgt aaatggtaat gtcgtagaaa
     1441 ctaaagaagt gtcacgaact gaagtagctc cggtcaacga agtcgttaaa gtaggaacac
     1501 ttgtgaaagt taaacctaca gtagaaatta caaacttaac aaaagttgag aacaaaaaat
     1561 ctataactgt aagttataac ttaatagaca ctacctcagc atatgtttct gcaaaaacgc
     1621 aagttttcca tggagacaag ctagttaaag aggtggatat agaaaatcct gccaaagagc
     1681 aagtaatatc aggtttagat tactacacac cgtatacagt taaaacacac ctaacttata
     1741 atttgggtga aaataatgag gaaaatactg aaacatcaac tcaagatttc caattagagt
     1801 ataagaaaat agagattaaa gatattgatt cagtagaatt atacggtaaa gaaaatgatc
     1861 gttatcgtag atatttaagt ctaagtgaag cgccgactga tacggctaaa tactttgtaa
     1921 aagtgaaatc agatcgcttc aaagaaatgt acctacctgt aaaatctatt acagaaaata
     1981 cggatggaac gtataaagtg acggtagccg ttgatcaact tgtcgaagaa ggtacagacg
     2041 gttacaaaga tgattacaca tttactgtag ctaaatctaa agcagagcaa ccaggagttt
     2101 acacatcctt taaacagctg gtaacagcca tgcaaagcaa tctgtctggt gtctatacat
     2161 tggcttcaga tatgaccgca gatgaggtga gcttaggcga taagcagaca agttatctca
     2221 caggtgcatt tacagggagc ttgatcggtt ctgatggaac aaaatcgtat gccatttatg
     2281 atttgaaaaa accattattt gatacattaa atggtgctac agttagagat ttggatatta
     2341 aaactgtttc tgctgatagt aaagaaaatg tcgcagcgct ggcgaaggca gcgaatagcg
     2401 cgaatattaa taatgttgca gtagaaggaa aaatctcagg tgcgaaatct gttgcgggat
     2461 tagtagcgag cgcaacaaat acagtgatag aaaacagctc gtttacaggg aaacttatcg
     2521 caaatcacca ggacagtaat aaaaatgata ctggaggaat agtaggtaat ataacaggaa
     2581 atagttcgag agttaataaa gttagggtag atgccttaat ctctactaat gcacgcaata
     2641 ataaccaaac agctggaggg atagtaggta gattagaaaa tggtgcattg atatctaatt
     2701 cggttgctac tggagaaata cgaaatggtc aaggatattc tagagtcgga ggaatagtag
     2761 gatctacgtg gcaaaacggt cgagtaaata atgttgtgag taacgtagat gttggagatg
     2821 gttatgttat caccggtgat caatacgcag cagcagatgt gaaaaatgca agtacatcag
     2881 ttgataatag aaaagcagac agattcgcta caaaattatc aaaagaccaa atagacgcga
     2941 aagttgctga ttatggaatc acagtaactc ttgatgatac tgggcaagat ttaaaacgta
     3001 atctaagaga agttgattat acaagactaa ataaagcaga agctgaaaga aaagtagctt
     3061 atagcaacat agaaaaactg atgccattct acaataaaga cctagtagtt cactatggta
     3121 acaaagtagc gacaacagat aaactttaca ctacagaatt gttagatgtt gtgccgatga
     3181 aagatgatga agtagtaacg gatattaata ataagaaaaa ttcaataaat aaagttatgt
     3241 tacatttcaa agataataca gtagaatacc tagatgtaac attcaaagaa aacttcataa
     3301 acagtcaagt aatcgaatac aatgttacag gaaaagaata tatattcaca ccagaagcat
     3361 ttgtttcaga ctatacagcg ataacgaata acgtactaag cgacttgcaa aatgtaacac
     3421 ttaactcaga agctactaaa aaagtactag gagcagcgaa tgatgcagcc ttagataacc
     3481 tatacttaga tagacaattt gaagaagtta aagctaatat agcagaacac ctaagaaaag
     3541 tattagcgat ggataaatca atcaatacta caggagacgg tgtagttgaa tacgtaagtg
     3601 agaaaatcaa aaataacaaa gaagcattta tgctaggtct tacttatatg aaccgttggt
     3661 acgatattaa ttatggtaaa atgaatacaa aagatttatc tacgtacaag tttgacttta
     3721 acggaaataa tgagacttca acgttggata ctattgtcgc attaggaaat agtggactag
     3781 ataacctgag agcttcaaat actgtaggtt tatatgcgaa taaacttgca tcggtaaaag
     3841 gagaagattc agtctttgac ttcgtagaag cgtatagaaa actgttctta ccaaacaaaa
     3901 caaataacga gtggtttaaa gaaaatacaa aggcatatat agtcgaaatg aagtctgata
     3961 ttgcagaagt acgagaaaaa caagaatcac caacagccga tagaaaatat tcattaggag
     4021 tttacgatag aatatcagca ccaagttggg ggcataagag tatgttatta ccactactaa
     4081 ctttacctga agaatctgtg tatatttcat cgaatatgtc tacacttgca ttcggttcgt
     4141 atgaaagata tcgtgatagt gtggatggag ttattctttc aggagatgct ttacgaactt
     4201 atgtaagaaa tagagttgat atagcagcga aaaggcatag agaccattat gatatttggt
     4261 acaatcttct tgacagtgct tcaaaagaaa aacttttccg ttctgtgata gtttatgatg
     4321 gattcaatgt aaaagatgag acaggaagaa cttattgggc aaggttaacg gataaaaaca
     4381 tcggctctat taaagaattc ttcggacctg ttgggaaatg gtatgagtat aatagtagtg
     4441 caggagcgta tgcgaatgga agtttaacgc actttgtgtt agatagatta ttagatgctt
     4501 atggaacgtc ggtttatact catgaaatgg ttcataattc tgattctgca atctactttg
     4561 aaggaaatgg tagacgtgaa ggattgggag cggagttata cgcacttggt ttactgcaat
     4621 ctgtagatag tgtaaattct catattttag ctttaaatac gttatataaa gcagaaaaag
     4681 atgatttgaa tagattgcat acatataatc cggtggaacg tttcgattcg gatgaggcgc
     4741 ttcaaagtta tatgcatgga tcatatgatg taatgtatac acttgatgcg atggaagcaa
     4801 aagcgatatt agctcaaaat aatgatgtta agaaaaaatg gtttagaaaa atagaaaatt
     4861 attacgttcg tgatactaga cataataaag atacacatgc aggaaataaa gtccgtccat
     4921 taacagatga agaagtagct aacttaacat cgttaaactc attaatcgac aacgacatca
     4981 taaatagacg tagctatgat gataatagag aatataaacg aaatggctac tatactataa
     5041 gtatgttctc tcctgtatac gcagcgctaa gcaattcgaa aggtgctcct ggagatatta
     5101 tgtttagaaa aatagcttat gaattacttg cggaaaaagg ttatcacaaa ggattcctac
     5161 cttatgtttc taatcagtac ggagcagaag catttgccag cggaagcaaa acattctcat
     5221 catggcatgg aagagatgtt gctttagtga cagatgattt agtatttaag aaagtattca
     5281 atggtgagta ctcatcatgg gctgatttca aaaaagcaat gtttaaacaa cgtatagata
     5341 aacaagataa tctgaaacca ataacaattc aatacgaatt aggtaatcct aatagtacaa
     5401 aagaagtaac tataacaacg gctgcacaaa tgcaacaatt aattaatgaa gcggctgcga
     5461 aagatattac taatatagat cgtgcaacga gtcatacccc agcaagttgg gtgcatttat
     5521 taaaacaaaa aatctataat gcatatcttc gcactacaga tgactttaga aattctatat
     5581 ataaataaga ttgtagagtt tcattgttga gtagtgtttc ttgtaaggat gaggagtcag
     5641 atgacaaatc gactcctttt tcttatggat cgatgtagag atttgattga atgcagattg
     5701 caggaatcat cttcaactca tcaacgacca atggtgacaa ggtggatttc aatcccacag
     5761 aaaatgttga tttgagaaat aactttgcta gtctagtaaa ataaatacaa aacaatccta
     5821 gaagattttt tctgggattg ttttttgctg agtgggatgc ttcaagttgt ctggcttgac
     5881 tttcttgagg gaagttatat aatggttgta ataattaaag ttacaataaa agaggaaata
     5941 tttatgacat atgaatacca gagccacatt tatttagcag aggcagtttt aaatgtgaag
     6001 gatttggtca gtcaaacagt tttttatcag cagattattg gtttagaaat cctatctcaa
     6061 acggatacag aggtcgttct gggacttgga ggaaaagcct tggtacactt gattcaagca
     6121 caagagggtg gagaagtaag ggaacattat ggtctttacc atctggctat tcttttgccg
     6181 acacgaaagg ctttggctga tgtcttgaag cacctgacgg atttacagat tcctcttgtt
     6241 ggcggtgcag atcacggtta cagtgaggcc ctttacttag aggacttgga gggaaatggc
     6301 attgaactct atcgagataa gccagtttcc acatgggata ttcgagaaga tggacgtatt
     6361 atcggggtga ctgaagtcct tgcggctcag gatatctatg agttggggga aagagtagag
     6421 ccttttatcc tagcagaggg tacgagaatg gggcatattc atctttctgt caaggatagt
     6481 cgaaagtcca gacagtttta tcaaacggtg ttagggctcg aggataaatt cagtgtgcct
     6541 agtgctagtt ggatcgcagc tggggactac catcatcatt tagcagtcaa cgaatgggga
     6601 ggaaaaggtc tggatccgcg taaacaagtc ctaccaggtt tagcctacta tgtcatcgaa
     6661 gtcgcacata aagaagaact gttaacgatt gcccaacgag cacaagaagt tgacgcacca
     6721 atcaaatgga tgacatcgat ccaattggaa atcacagact cagatggcat cgtgacccgt
     6781 attcgtttag ctagatagat ggtatgtgat gaaggtagag catcaattgt agaaagtgct
     6841 ccttattcta gtcagatacg tggaaaattt gatatgatag tagggaataa ggagaagaat
     6901 aatatgacta gcgaatacca gaaaatgata gcaggagagt tttaccgtcc atcagaccca
     6961 gaactgagag ccttagctca ggcttctcgc caaaaacagg ccgcctttaa caaggaagag
     7021 aaccccttga agggagccga aatcatcaag acttggtttg cctcaaccgg gaaaaatctt
     7081 tacatcaaca ctcgcttgat ggtggactac ggtgtcaaca tccatctagg ggaaaatttt
     7141 tattctaatt ggaacttgac catgctggat atctgtccca ttcgtatcgg ggacaatgct
     7201 atgattggtc ctaattgtca gtttttgaca cccctccatc cactagatcc acaggaacgc
     7261 aattcaggta tcgagtacgg aaagcctatc acaatcggag ataatttctg gactggtggt
     7321 ggcgtcattg tccttcctgg agtgacactg ggaaataatg tcgttgcagg agcaggggca
     7381 gtaattacca aatcttttgg cgacaacgtt gtcctagctg gcaatcctgc gcgcgtgatt
     7441 aaggaaatac ctgttaaata gaagtaaaaa ggaacagctg gggttgtttc ttttttgtag
     7501 gtttcatcat tttttaccca gttcacattt acctactcta tctcttagca agtctgtttc
     7561 attaagcaag ttcaaagcat ctcgtaagtg ggatgttttt ctcctcagtt catcagcttc
     7621 ctccttgaca ctcggtcaga ttttgataca atagtacaaa attagaggag gcaggctatg
     7681 attcagaaac atgcgattcc tattttagag tttgatgaca atcctcaggc ggttatcatg
     7741 cccaatcacg aggggctgga cttgcagttg ccaaagaagt gtgtttatgc atttttaggt
     7801 gaggagattg accgctatgc gagggaagta ggggcgaact gtgttggcga atttgtttct
     7861 gccaccaaga cctatccagt ttatgtcgtg aactacaagg acgaggagat ttgtctggtt
     7921 caggctcctg tcggttcagc tccagcagcc cagtttatgg attggttgat tggctatggt
     7981 gtggagcaga ttatctctac tgggacctgt ggtgtcctag ctgatataga ggaaaatgcc
     8041 tttctagtcc ctgttcgcgc tctgcgagat gaaggagcca gttaccacta tgtggcacct
     8101 tctcgttata tggaaatgca gccagaggct attgctgcca ttgagcgagt tttggatgat
     8161 agagggattc cttatgaaga agtcatgacc tggtccacag atggttttta ccgagaaacg
     8221 gctgaaaagg tggcttatcg caaggaagaa gactgtgctg ttgtggagat ggagtgttcc
     8281 gctcttgcgg cagtagctca attgcgtggg gttctctggg gtgaattgtt gttcacagca
     8341 gattctctag cggacttgga ccagtacgac agtcgtgact ggggctcgga agcttttaat
     8401 aaggcgctag aactgagttt agcaagtgtt caccaccttt agttgtactg gcaaaggatt
     8461 tgttttatca taaaatgtct agctcatact tttcaaaaat atgtttaaac gaggtcacct
     8521 tcctcttgtc ctaggcatgt tgaggttggg aaaaatcttt aaaatcagaa aaacgtatca
     8581 tatcaggtga tgaaaacttt gacactatgc gttttatgtc gataagattt agagtgagat
     8641 gaaatgatac tcttcgaaaa tctcttcaaa ccaggtcagc ttcaccttgc cgtaggtata
     8701 tgttactgac ttcgtcagtt ctatttgcaa cctcaaaaca gtgttttgag caacctgtga
     8761 ctagctttct aatcgatgcc ttggttttca ttgcctataa tcaaaaagag aaattttctc
     8821 ctgaaaagca tatagagtag ctggcgttaa aagctcctgt cttgcttttt tgacctatag
     8881 tcacatctat caagtattgt tcttgcctaa gctatcaata aaaaggtggc attttttagg
     8941 cttggtgtta gtagattttg ccttatccta tctaagtcat ttcgaacttt ttatggtaca
     9001 atggaaacat gttattcaaa ttatctaagg aaaaaataga gctaggctta tctcgtttat
     9061 cgccagcccg tcgtattttt ttgagttttg ccttggtcat tttactaggc tctcttcttt
     9121 tgagcttgcc ctttgtccaa gttgaaagct cacgagcgac ttattttgat catcttttca
     9181 ctgctgtatc tgcagtctgt gtgacgggtc tctcaaccct tccagtagct cacacctata
     9241 atatatgggg tcaaataatc tgtttgctct tgattcagat cggtggtcta gggctcatga
     9301 cctttattgg ggttttctat atccagagca agcaaaagct tagtcttcgt agccgtgcaa
     9361 ctattcagga tagttttagt tatggagaaa ctcgatcttt gagaaagttt gtctattcta
     9421 tttttctcac gacctttttg gttgagagct tgggagctat tttgcttagt tttcgcctta
     9481 ttcctcaact tggctgggga cgtggtcttt ttagttccat ttttctagcg atctcagcct
     9541 tctgtaatgc cggttttgat aatttaggga gcaccagttt atttgctttt cagaccgatt
     9601 tactggtcaa tctggtgatt gcaggcttaa ttattacagg cggccttggt tttatggtct
     9661 ggtttgattt ggctggtcat gtaggaagaa agaaaaaagg acgtctgcac tttcatacga
     9721 agcttgtact attgttgact ataggtttgt tgttatttgg aacggcaact actctctttc
     9781 ttgagtggaa caatgctgga acgattggca atctccctgt tgccgataag gttttagtta
     9841 gcttttttca aacagtgacg atgcgaacag ctggcttttc tacgatagat tatactcagg
     9901 ctcatcctgt gactcttttg atttatatct tacagatgtt tctaggtggg gcacctggag
     9961 gaacagctgg gggactcaag attacgacat tttttgtcct cttggtcttt gcacgaagtg
    10021 agcttctagg cttgcctcat gccaatgttg cgagacgaac gattgcgccg cgaacggttc
    10081 aaaaatcctt tagtgtcttt attatctttt tgatgagctt cttaatagga ttgattctgc
    10141 tagggataac agccaaaggc aatcctccct ttatccacct catatttgaa accatttcag
    10201 ctcttagtac agttggtgta acggcaaatc tgactcctga ccttgggaaa ttggctctca
    10261 gtgttatcat gccacttatg tttatgggac gaattggtcc cttgaccttg tttgttagct
    10321 tggcagatta ccatccagaa aagaaagata tgattcacta tatgaaagca gatattagta
    10381 ttggttaaga aaggaaagag catgtcagat cgtacgattg gaattttggg cttgggaatt
    10441 tttgggagca gtgtcctagc tgccctagcc aagcaggata tgaatattat cgctattgat
    10501 gaccacgcag agcgcatcaa tcagtttgag ccagttttgg cgcgtggagt gattggtgac
    10561 atcacagatg aagaattatt gagatcagca gggattgata cctgcgatac cgttgtagtc
    10621 gcgacaggtg aaaatctgga gtcgagtgtg cttgcggtta tgcactgtaa gagtttgggg
    10681 gtaccgactg ttattgctaa ggtcaaaagt cagaccgcta agaaagtgct agaaaagatt
    10741 ggagctgact cggttatctc gccagagtat gaaatggggc agtctctagc acagaccatt
    10801 cttttccata atagtgttga tgtctttcag ttggataaaa atgtgtctat cgtggagatg
    10861 aaaattcctc agtcttgggc aggtcaaagt ctgagtaaat tagacctccg tggcaaatac
    10921 aatctgaata ttttgggttt ccgagagcag gaaaattccc cattggatgt tgaatttgga
    10981 ccagatgacc tcttgaaagc agatacctat attttggcag tcatcaacaa ccagtatttg
    11041 gataccctag tagcattgaa ttcgtaaaga gggatgaccc ctcttttttg atgcccaaga
    11101 tggcaaatag agacagaagc cccttgtctt ctagtaaaag ttcttcaaag gctggacttt
    11161 atggtaaaat agaaggaagt gacaagagag agtaatactc aatgaaaatc aaagatcaaa
    11221 ctaggaaact agctacgggc tgcttaaaac actgttttga ggttgtggat agaactgacg
    11281 aagtcagctc aaaacactgt tttgaggttg cagatagaac tgacgaagtc agtaacatct
    11341 atacggcaag gcgatgttga cgcggtttga agagattttc gaagagtata agaaaaaatc
    11401 agtcccctaa aggagcagat tatgaagtta ttgtctatcg ccatttctag ctataatgca
    11461 gcagcctatc ttcattactg tgtggagtcg ctagtgattg gctgggacca ggtcggaaat
    11521 ttctccaaag gccagtatat catgatgcac tcgctgattt atcggacaga tttgttgcgt
    11581 gctagccagt tctaattgcc tgaacatact ttttatgtcg ataatctctt tgtctttacg
    11641 ccccttcagc aggtcaagac catgtactat ctgcctgtcg atttctatcg ttatttgatt
    11701 gggcgtgagg gccagtctgt caatgagcaa gtgatgatta agtgcattga ccagcaactc
    11761 aaggtcaatc gactcttggt agaccagctt gatttgtccc aagtgagtca tcccaaaatg
    11821 cgagaatatc tgctgaatca tattgaaatc acgacggtga tttccagtac cctgctcaac
    11881 cgatctgaaa cagcggagca tctggcaaaa aaacgccaat tgtggaccta tattcagcag
    11941 aaaaatccag aagtctttca ggctattcgt aagaccatgt tgagccgttt gaccaaacat
    12001 tctgtcttgc cagatcgcaa actgtccaat gtcgtctatc aaatcaccaa atctgtttat
    12061 ggatttaatt aatataagtg ttttataaga gggatttaag aaaaatttta actttttctt
    12121 agtccttttt aatttcagga gattatacta gagtcatcaa ataaagaaag actctaagga
    12181 gaatcctatg aaattcaatc caaatcaaag atatactcgt tggtctattc gccgtctcag
    12241 tgtcggtgtt gcctcagttg ttgtggctag tggcttcttt gtcctagttg gtcagccaag
    12301 ttctgtacgt gccgatgtgg tcaatccgac cccaggtcaa gtcttacctg aagagacatc
    12361 gggaacgaaa gagggtgact tatcagaaaa accaggagac accgttctca ctcaagcgaa
    12421 acctgagggc gttactggaa atacgaattc acttccgaca cctacagaaa gaactgaagt
    12481 gagcgaggaa acaaactctt ctagtctgga tacacttttt gaaaaagatg aagaagctca
    12541 aaaaaatcca gagctaacag atgtcttaaa agaaactgta gatacagctg atgtggatgg
    12601 gacacaagca agtccagcag aaactactcc tgaacaagta aaaggtggag tgaaagaaaa
    12661 tacaaaagac agcatcgatg ttcctgctgc ttatcttgaa aaagctgaag ggaaaggtcc
    12721 tttcactgcc ggtgtaaacc aagtaatccc ttatgaacta ttcgctggtg atggtatgtt
    12781 aactcgtcta ttactaaaag cttcggataa tgctccttgg tctgacaatg gtactgctaa
    12841 aaatcctgct ttacctcctc ttgaaggatt aacaaaaggg aaatacttct atgaagtaga
    12901 cttaaatggc aatactgttg gtaaacaagg tcaagcttta attgatcaac ttcgcgctaa
    12961 tggtactcaa acttataaag ctactgttaa agtttacgga aataaagacg gtaaagc
//
LOCUS       DAVHEW010000052        12591 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVHEW010000052 DAVHEW010000000
VERSION     DAVHEW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.052"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            473..1594
                     /gene="mnmA"
                     /locus_tag="VVL53_001360"
     CDS             473..1594
                     /gene="mnmA"
                     /locus_tag="VVL53_001360"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU4183525.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1735..2190
                     /locus_tag="VVL53_001361"
     CDS             1735..2190
                     /locus_tag="VVL53_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4183526.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPVFLKTALPDWEGQLRHIHLEE"
     gene            2200..4113
                     /gene="mnmG"
                     /locus_tag="VVL53_001362"
     CDS             2200..4113
                     /gene="mnmG"
                     /locus_tag="VVL53_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU4183527.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4320..4412)
                     /locus_tag="VVL53_001363"
                     /pseudo
     CDS             complement(4320..4412)
                     /locus_tag="VVL53_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4466..6145)
                     /locus_tag="VVL53_001364"
     CDS             complement(4466..6145)
                     /locus_tag="VVL53_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU4183528.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6147..6380)
                     /locus_tag="VVL53_001365"
     CDS             complement(6147..6380)
                     /locus_tag="VVL53_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU4183529.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6660..6875
                     /locus_tag="VVL53_001366"
     CDS             6660..6875
                     /locus_tag="VVL53_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183530.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VVL53_001367"
     CDS             complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VVL53_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU4183531.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VVL53_001368"
     CDS             complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VVL53_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU4183532.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7715..8398
                     /gene="tsaB"
                     /locus_tag="VVL53_001369"
     CDS             7715..8398
                     /gene="tsaB"
                     /locus_tag="VVL53_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU4183533.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8395..8832
                     /gene="rimI"
                     /locus_tag="VVL53_001370"
     CDS             8395..8832
                     /gene="rimI"
                     /locus_tag="VVL53_001370"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU4183534.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMTVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8822..9832
                     /gene="tsaD"
                     /locus_tag="VVL53_001371"
     CDS             8822..9832
                     /gene="tsaD"
                     /locus_tag="VVL53_001371"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU4183535.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKILVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9878..11178)
                     /locus_tag="VVL53_001372"
                     /pseudo
     CDS             complement(9878..11178)
                     /locus_tag="VVL53_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11454..11805)
                     /locus_tag="VVL53_001373"
                     /pseudo
     CDS             complement(11454..11805)
                     /locus_tag="VVL53_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11912..12205)
                     /locus_tag="VVL53_001374"
                     /pseudo
     CDS             complement(11912..12205)
                     /locus_tag="VVL53_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3564 a         2503 c         2932 g         3592 t
ORIGIN      
        1 ggcttaggtg cccttgcagt caacacaact gtagatggct ataaagtcaa tgccaatggt
       61 gaatgggttt aagccgatta aattaaatca tgttaagaac atttgacatt ttaattttga
      121 aacaaagata aggttcgatt gaatagattt atgttcgtat tctttaggta cctatcttat
      181 gatttcagga aatgtcatta aaaaaacgac tcattttctc taacttgaaa aatagattag
      241 agaaaatggg ttgttttatt tattatagtt atttgaatga agataagaag aaggtatact
      301 cacatcattc acataatctg tatattgact ataagtttta aaaaacaatt tttaagctct
      361 tccttgtctt ctctaaccaa gcgtgttata atgaatactg ctcaagcgac cttcaatcgt
      421 gaagcacaca cgaccttcaa tcgtgaataa acgaatagat gggagactta ccatgagtga
      481 taactctaaa acacgtgttg tcgtggggat gagtggtggt gttgattcgt cggtgacggc
      541 tcttttgctc aaggagcagg gctacgatgt gatcggtatc ttcatgaaga actgggatga
      601 cacagatgaa aacggcgtct gtacggcgac cgaagattac aaggatgtgg ttgcggtggc
      661 agaccagatt ggcattccct actactctgt caattttgaa aaagagtact gggaccgcgt
      721 ttttgagtat ttcctagcgg aataccgtgc agggcgcacg ccaaatccgg acgttatgtg
      781 caacaaggaa atcaagttca aggccttttt ggactatgcc ataaccttgg gggcagacta
      841 tgtagcgact gggcattatg ctcgagtggc gcgtgatgag gatggtaccg ttcacatgct
      901 tcgtggcgtg gacaatggca aggatcagac ctatttcctc agccaacttt cgcaagaaca
      961 acttcaaaaa accatgttcc cactaggaca tttggaaaag cctgaagtac gcagactagc
     1021 agaagaagca ggcctttcga ctgctaagaa gaaagactcg acagggattt gctttatcgg
     1081 agaaaagaac tttaaaaact ttctcagcaa ctacctgcca gctcagcctg gtcgcatgat
     1141 gactgtggat ggtcgcgata tgggcgagca tgcaggtctt atgtactata caatcggtca
     1201 gcgtggcgga ctcggtatcg gtgggcaaca cggcggtgac aatgcccctt ggttcgttgt
     1261 cggaaaagat ctaagcaaga atattctcta tgtaggacaa ggattctacc atgattcgct
     1321 catgtcaact agcctagaag ccagtcaagt ccactttact cgtgaaatgc cagaagagtt
     1381 tacgctagaa tgtacggcta aattccgtta ccgtcagcct gactctaagg tgaccgttca
     1441 tgtcaaagga gaaaagacag aggtcatctt tgcggaacca caacgcgcga ttacaccagg
     1501 acaggcagtt gtcttttacg atggcgaaga gtgtctaggt ggcggtttga ttgacaatgc
     1561 ttaccgcgat ggacaagttt gtcagtacat ttagattgac aaattttctc aatttgctac
     1621 aataataaca gcaatagaaa tgatggtcaa agctcatgga tgttgcaggc ttttttgtcc
     1681 tgcacttctt tgtagttttg actgtttttg tgtcgtttaa gggaaaggac aagaatgact
     1741 caacaagact ttcggacaaa agtagacaat acggtttttg gtgttcgggc tacagccttg
     1801 attgtccaaa atcacaagct tctagttacc aaagacaagg gcaagtatta cactattgga
     1861 ggtgcgattc aagtcaatga aagcacggaa gacgcggtag tccgtgaagt gaaggaagaa
     1921 ctgggtgtca aagctcaagc tgggcagcta gcttttgtgg ttgaaaatcg ttttgaagtg
     1981 gacggcgttt cctatcacaa tatcgagttt cattatctgg tggatttgct tgaagatgcc
     2041 ccgttgacca tgcaggaaga tgagaaaagg cagccctgtg agtggattga cttggataag
     2101 ctccagaata tccagctagt tccagtcttt ttaaaaacag ccctaccaga ttgggaaggc
     2161 caactaagac acattcatct tgaggaatag gagagaaaca tgacttatca ttttactgaa
     2221 gaatacgata ttattgtaat tggtgcggga cacgctgggg ttgaggcttc cttggccgct
     2281 agccgtatgg gctgtaaggt cctgcttgcg accatcaata ttgaaatgct ggctttcatg
     2341 ccttgtaatc cctctatcgg tggttctgcc aaggggattg tcgtgcgtga agtcgatgcc
     2401 ctcggtggcg agatggccaa aaccattgac aagacttaca tccagatgaa gatgctaaac
     2461 acagggaagg ggccagctgt ccgtgccctt cgtgcgcagg ctgacaagga actttactct
     2521 aaggagatgc gcaagacggt tgaaaaccaa gaaaatctga cccttcgtca aaccatgatt
     2581 gatgagattt tggtggaaga tggcaaggtt gtcggtgtgc gtacagccac ccatcaagaa
     2641 tatgctgcta aggctgttat tgtgacgaca gggactgctc tccgtgggga aattatcatc
     2701 ggagacctca agtactcatc aggtcctaac cacagcttgg cttctattaa cctagctgac
     2761 aatctcaagg aactgggtct cgaaatcggt cgtttcaaga caggaacccc tccacgtgtc
     2821 aaggcttctt ctatcaatta cgatgtgaca gaaattcagc caggagacga agtgcctaat
     2881 catttctcat acacttcacg tgatgaggat tatgtcaagg accaagtacc atgctggttg
     2941 acctatacca atggtaccag tcatgagatt atccaaaaca acctccaccg tgcgcctatg
     3001 tttacaggtg tggtcaaggg agtggggcct cgttactgtc cgtcgattga agacaagatt
     3061 gtgcgctttg cggacaagga acgtcaccaa ctcttccttg agccagaagg gcgcaatact
     3121 gaggaagtct atgtgcaagg actttcaacc agtctgcctg aggatgtcca gcgtgacttg
     3181 gtgcattcca tcaaaggttt ggaaaatgca gagatgatgc ggacaggtta tgctattgag
     3241 tatgatatgg tcttgcctca tcagttgcgt gcgactttgg aaaccaagaa aatctcaggt
     3301 ctcttcactg ctggtcagac aaatggaaca tcaggttacg aagaggcagc aggccaaggg
     3361 attatcgcgg gtatcaatgc ggctctgaaa atccaaggca agcctgaatt gattttgaag
     3421 cgcagtgatg gttatatcgg ggtgatgatc gacgacttgg tgaccaaggg aaccattgaa
     3481 ccctaccgtc tcttgaccag tcgtgctgaa taccgtctca ttcttcgtca tgacaatgct
     3541 gatatgcgct tgactgagat gggacgcgag attggccttg tggacgatga acgctgggct
     3601 cgttttgaaa tcaagaaaaa tcaatttgat aatgagatga agcgcctaga cagtatcaaa
     3661 ctcaagccag tcaaggaaac caatgccaag gttgaggaga tgggcttcaa acccttgacc
     3721 gatgcagtga cagccaagga attccttcgc cgtccagaag tttcttacca agatgtggtg
     3781 gccttcatcg gaccagctgc agaagacttg gatgacaaga ttatcgaatt gattgaaaca
     3841 gaaatcaagt atgaaggcta tatttccaaa gccatggacc aggttgccaa gatgaaacgc
     3901 atggaagaaa aacgcattcc ggccaatatc gactgggatg acattgattc tatcgcaacc
     3961 gaagcccgtc agaagttcaa actcatcaat ccagaaacca tcggccaagc cagccgtatt
     4021 tcgggagtaa acccagcaga tatttctatt ttgatggtgt atctggaagg taaaaatcgt
     4081 agtatttcta aaactcttca aaaatcaaaa tgatacgtcg tcggcttctt acgaatgagt
     4141 tcaaagcttg gctttgattc atctccagcc tcccatagtt tcccgaacta tgggagctaa
     4201 ctcaagttat gctttcgctt agccttctag tctgatttcg atttttattg atactcttcg
     4261 aaaatctctt caaaccgcgt caacgtcgcc ttgccgtagg tatggctact gacttcgtca
     4321 gttctatcta caacctcaaa gcagtgcttt gagcaaccta cggctagctt cctagtttgc
     4381 tctttgattt tcattgagta tgagttttaa tctcaaaaga aagcatagag aaaaacagct
     4441 ccgaggacgg ggctgttttc ttgctttatt cttcatctgg tgtgaggatc atcgggatga
     4501 tgatcggttc acgttcggta ttttcataga ggaaggggcg aatagcgttg acaatggcac
     4561 cattgacaga ttgcacgcta gcatccttat ttttcagtgc gatacgaatg gcattgaaga
     4621 ggatacgctg gctttggcga atcaagtcgc cagactctct catgtagaca aagcctcggc
     4681 tgaggatgtc tggaccagat agaatcatct gcgatttgaa gtcaacagtt gcaactgcca
     4741 gaacgacacc gtcttcagat agatcgcgac gatctttgag gacagctgcg ccaatttcac
     4801 cgatacgatt tccatcgaca tagatatctt gggcgttgaa atgacctgcg atacgagctg
     4861 agtcagcagt aagggcaagc acatcgccat tgctcatgat aaagatattg tccttctcaa
     4921 caccagtatc cactgctagt ccagcgtgga ctttttgcat gcggtattca ccgtggacag
     4981 gcatgaagta ttttggctta atcaagcaga gcatgagttt ttgctcttgc tgaccaccgt
     5041 gtccagatgt atggatattg ttcactttac cgtggataac ttcgacacca gcttcagaaa
     5101 tgatgttaat cagcttgttg acactagtag tgtttccagg gatgggacta gaagagaaga
     5161 taacggtatc acctggttgt aattgtactt gacggtgggt tccgttggcg atacgagaga
     5221 gggctgccat aggctcaccc tgactacctg tacagaggat aagaacttct cctgcaggat
     5281 aatctttgat ttcatttggc tcgataaagg ttcccttagg agctttgatg tagccaagat
     5341 cgattccgtt gacaatggcc ttttccatag aacgaccaaa gaccgcaatc ttgcgtccag
     5401 tcttaacagc agcttctgtt gcctgctgga gacggaagat atttgaggca aaggatgcaa
     5461 agatgatacg tccttcaata ccttggataa tcttcataat ggactgacca acgacttttt
     5521 cagagttggt aaaggttggt acttccgcat ttgtcgagtc agacaggaga cagagcacgc
     5581 cttcttcacc aagcgcagcc atacgatgca agtccgcagg ttctccaact ggagtaaagt
     5641 cgaacttaaa gtcacccgta cagacgattt tcccttgagg agtatgaatg acaatcccca
     5701 aaggctctgg aatagagtga gtcgttctaa agaaagttgc cttgagattt ttaaaggtca
     5761 actcggtgtt gtggttgatt tcgtaaagtt tggcgttgcg caagaggccg tgttcttcga
     5821 gtttcccacg gatcaaagcc aaggcaagcg gtccagcata aatagggaca tttgcttgct
     5881 tgagtaggaa cggaatccca ccaatgtggt cctcgtgtcc gtgtgtgatt aaaacagcct
     5941 tgacgcggtc gatattgtcc acgatgtaag agtagtcagg aatgacatag tcgataccaa
     6001 gcaagtcatc ttctgggaat ttaatcccag catcgacgat gataatctcg tcttggtatt
     6061 caattccgta agtgtttttc ccgatttctc ctagaccacc gatggcaaaa acgccgactt
     6121 cttcaggttt aagagtgtag gccatattag aactccgtaa tttcgaaggc gccagtttct
     6181 ttttcgtaat cgagcaattt gtcagacaag agttcgatat actcgatatt gtactctggg
     6241 cgattttctt cgacaagttg gcgagcagtg atacggccct caagttctga gctggcatcg
     6301 atgtctaggt aaagcgtgcg tgttgtttca cggcgtgggc tacgttcttt tgtttcttga
     6361 taaaaaactt tgtaaatcat atagttcctt tctatttaca ggtcagctta ttccaaactt
     6421 ttagcagata tttgcttgat ttcattccat tttgtgtcta gattgacctt gattcgttac
     6481 aattatttca attataccac aaaatgaaaa attagtaaaa gacggaaacg agaaagttag
     6541 gggacgattt ctttggtaag cgttttcaaa aataaagaaa atgtattatg ttgataacgg
     6601 aagtgaaatt tattgcttgt cataattcca tatagatttt aagtcaagga ggttgcccta
     6661 tgtataactt atttgctttt gctatcggag ctcgcttggt agcttttatt gtttttgttg
     6721 cttgcgttta tgcggggaat cttctatttg ccaaactgga acgacgacaa accaagttct
     6781 tgtggaaaat gacctttgtg actctctact cgctttttct cctcctcgtg acctatgtcg
     6841 tttggtttgt attttacttt gaattaaatg cttagatgcc ctgcttatgc tggggttttt
     6901 tgtttgggaa tgtagagata gtagatttac gacacgaaaa ttccacccca tctatcaaga
     6961 tgaggtggtc tttgcatttt tctaacatga catgacaatg gaattttcaa agcttattat
     7021 ttttatggga gtcattgttc tgatcaaaaa tagagttgaa gcataaaaat aaaacaatac
     7081 aaacagataa atcgaatggt aaagggatgg tagtaatgtt caaatcattg agaatgctga
     7141 gcaaactgta agagatgaag acaataaaat ttatccttct aaaaatgtgt ttcttatcta
     7201 gcatgactta ccaaactttt tacgaaggtc ttttccaatg gtataacctg ccataaataa
     7261 agcgatacca acaccgacac caaagccacc cttgatttct tgcaattcct tattagaaat
     7321 ttcacgataa ttgttcaaat ctttcatcat attaatctcc tcctaaaaaa taaccagctg
     7381 ccaaaccaga acctataagt gctgttccac ctgcaatagc cttccaagat actgctactc
     7441 caaagataac caagggagcg agaccgccat caatatctga taattcattt tcagataagg
     7501 ataaaaattg attgtcaaga atgtcaaaat ttgtcattga actttcctcc ttttttcttg
     7561 ttcactttta tattcggaaa agaaaaggaa aagtgagcat ttttttaaat tatttttagt
     7621 taagtgaggg cactcttgtc tgggggcaaa gaaaattccc atgtgccagc ttttgtagta
     7681 taatagtaag cagacaaaaa gataggaatg tgttatgaaa gtattagctt ttgatacgtc
     7741 cagcaaggct ctttctctgg ctattttaga ggataagcag gttcttgccg agacgacgat
     7801 taatattaag aaaaatcaca gtattactct tatgcctgcc atcgattttt tgatggcaag
     7861 tttggattgg acacccaagg atttggaccg aatcgtggta gctgaagggc cgggtagcta
     7921 tacaggcttg cgaattgcgg tagcaactgc taagacctta gctcacaccc tgaacatcga
     7981 gttggttggt atgtcgagtc tcttggctct ggtgccccat caacaagaag gtttgtttgt
     8041 ccccttgatg gatgcgcgtc gcaataatgt ttatgcagga ttttatgaaa atgccaaacc
     8101 tgtcatggca gaagcgcacc tatcttttga agaggtgcta gaaaaagtca agggtactag
     8161 tcaggtaacc tttgtcggag aagttggccc ctttgttgag cagattcaaa aacacttgcc
     8221 aaggactgat tacaaagaaa cattgcccaa tgcagctaat ctagctcttt tggcctggga
     8281 caaggaagca gactccttgc atgattttgt gccgaattac ctcaaacgag tcgaggctga
     8341 ggaaaactgg ctcaagaacc ataccgagtc tggcgagtct tacattaaac gcctatgata
     8401 gaaatcaagc gaattcaaca acagcctgac ctagctcaag ccatctacgc tgttatggca
     8461 gctgtttacc tagtcagtcc ttggactctg gagcaaatcc aagcagatct gtcccaagac
     8521 cagacttggt atgcattggc ttatgatggg gcagaagtga ttggatttct agctgtgcag
     8581 gagaatcttt ttgaagcaga agtcctgcaa atcgctgtca aaggagctta tcagggtcag
     8641 gggattgcgt cagccttgtt tgctcaattg ccgacagaca aggaaatttt cctcgaagtc
     8701 agacagtcaa atcaacgagc gcaagcattt tacaagaaag aaaagatgac agttatcgct
     8761 gagcgaaagg cctactacca tgacccagtc gaggacgcca ttatcatgaa gagagaaata
     8821 gatgaaggat agatatattt tagcatttga gacatcctgt gatgagacca gtgtcgccgt
     8881 cttgaaaaac gacgatgagc tcttgtccaa tgtcattgct agtcaaattg agagtcacaa
     8941 acgttttggt ggcgtagtgc ccgaagtagc cagtcgtcac catgtcgagg tcattacagc
     9001 ctgtatcgag gaggcattgg cagaagcagg gattaccgaa gaggacgtga cagctgttgc
     9061 ggttacctac ggaccaggct tggtcggagc cttgctagtt ggtttgtcag ctgccaaggc
     9121 ctttgcttgg gctcacggac ttccactgat tcctgttaat cacatggctg ggcacctcat
     9181 ggcagctcag agtgtggagc ctttggagtt tcccttgcta gccctcttgg tcagcggcgg
     9241 acacacagag ttggtttatg tttcggaggc aggagattat aagattgttg gggaaacccg
     9301 tgatgatgcg gttggtgagg cttatgataa ggtcggccgt gtcatgggct tgacctatcc
     9361 tgcaggtcgt gagattgacg agctggctca tcaggggcag gatatttatg atttcccccg
     9421 tgccatgatt aaggaagata atctggagtt ctccttctca ggtttgaaat ctgcctttat
     9481 caatcttcat cacaatgccg agcaaaaggg agaaagcctg tctacagaag atttgtgtgc
     9541 ttccttccaa gcagcagtta tggacattct catggcaaaa accaagaagg ctttggagaa
     9601 atatcctgtt aaaatcctag ttgtggcagg tggtgtggca gccaataaag gtctcagaga
     9661 acgcctagca gccgaaatca cagatgtcaa ggttatcatc ccccctctgc gactctgcgg
     9721 agacaatgca ggtatgattg cctatgccag cgtcagcgag tggaacaaag aaaacttcgc
     9781 aggctgggac ctcaatgcca aaccaagtct tgcctttgat accatggaat aaagagtggc
     9841 tctttgtcaa gtgtagtggg taggcgaaaa gctgcaatct ggagagtacg aaattcgtac
     9901 tctctttttt gatgttcaaa gtcatcaaaa tccgtttttt aaaattgtta aagttccgga
     9961 aaccaaaggc tttgcgcttg ataatcttaa tcaaattatt ggtcgcttca agtttagcgt
    10021 tggaataagg cagcttaagt gcgttgataa tcttatctct atcccttaaa aaagtccaaa
    10081 agaccgtttg aaaaagcgga tgaaccgttg gcagttcttg ctcaatcaat ccaaagaaat
    10141 ggtcggcatt cttctcttga aaatgaaaga gcaggagttg atagagttcg tagtgaacct
    10201 gtaatccctc agagtaagaa agcagcttat ctcgtacttc ccgatgggtt aagtgcattc
    10261 gaaacgtggg ctgatagaat cgtttctgac agagcttgaa gctatactgg tgtagtaaat
    10321 cagtccagtg gactgattta gcccgagcct agaaacgaaa aagcgagggt tccaaaagcg
    10381 cttgagcgcc cgatactcca aggattttcg gtcaaattgg ttcatgattt ggattcgtac
    10441 tcggttcata gctcggctca gatgttggac aatgtggaag cggtcaagaa caatcttcgc
    10501 ctttggaaag agttgcttag cgagccgata ataagggctg tacatgtcca tggtgatgac
    10561 ctcgaccagc tcccgaacct ctctctgata gcgttggaaa tggtttcgaa tcaccgcatg
    10621 agttcgcccg tctaaaattg cgaggatgga tttggactca aaatcttggg caatgaagct
    10681 cattttgctc tttttgaagc tatactcatc ccaactcata acttttggca acttggtcca
    10741 atcggtttca aacttaaatt ccctcagttt tcggatgacg gtggaagttg agaccgccaa
    10801 tctgtgggca atctcagtca tagactgctt ttcaaggagg agctgagcga ttttttgtct
    10861 caccatgttg gaaatctggc aatttttctt gacaatggat gtttgagaaa cgaccacctt
    10921 aaggcaattc ttgcactgaa agcggcgctt tttgagatgc agtaccgtgg gtaaaccctg
    10981 acagtcgaga agcggaattt tagaggcttt ctggaagtcg tatttgatca tcttcccttg
    11041 acaatgagga caaggaggag cgggggaatc caactttgcc tgaacgacta gatgggtttg
    11101 gtatttcaga acagacaaga ttttgatatt tttgtcttcc aatccgagca aatctgtggt
    11161 attcttaagt tgttccatat gaaccctttc taatgagttg gttagacgct tttcattata
    11221 ggtcatatgg ggcttttttt ctacaagaaa cgaccctata attcctgggg tgggattacc
    11281 cactacagaa attatagagc caaaacattc caaagtcttg tctgactctg gaatgttttc
    11341 tgttcacagt tgttttgtaa ctgcctaata agtaaccgcg ctataacgag gtatctaccg
    11401 agattgtcaa ttctccagta tactgttata aaaaacaaaa ctggaggatt tttttatgtc
    11461 cccccatcgt cccatcgact gttccgtagc tgcccaataa cggagtatag tcggctgaaa
    11521 caagaatagg acaaaagtgc ctcaagaaaa gcatcgtaat ttgacaatat tattctgaga
    11581 tgtttttgat gtaagcccat attttctcaa tgggattata ctcgggtgaa taggtaggaa
    11641 gtggtaacag tctatgccct gctccttaca catgttcatt ctgtgaaacc ttgcattgtc
    11701 cataatgata agggatggtg tatctaaagt aggtagtaag aatttttgga agcaagcttc
    11761 gaaaaagtca ctcgtcatcg tatctttata ggtcatcgga gccatagtat attgaaacaa
    11821 aaaagggcat tagtacttct ataacagtat cgcaatctct ggtgtgcgtt gtctcgatct
    11881 taattcaaat aattatatct tcttccagag agactaactg cctgctcaag gctcgatcat
    11941 attcgagata aaaataggtc tcaacccctg tctcataaat ataaatagga gtcaggtagc
    12001 ttaagtgatt caattcttta aggaaccgat ttactttttc tgggtcttgt tcgcagtagg
    12061 tacggctctt ttttagacta tatcccatag cctttagagc gtaatgaata gttgttggag
    12121 gacagttgaa ttcagcagct atttcagtca aataagcatc gggatgagtt tcaatataga
    12181 tttttaattt atctctatca attttttcaa ttaatagtgg aagttttctt gaagttagtg
    12241 tattgaaacg tttttataca gtgctaaaat ttgaagtgta gtgcagatgg atttgtaata
    12301 gtattatagc aattctttgg agaaggtcga gacaagataa aataggtatc tttgagatat
    12361 aagcatgatt tactataaaa ttattgagtt attcataaaa atttctctat caaatatcat
    12421 aatgcgtcgt ttaaggagat ggttacaatg caatatagtt gtggaaaaat aaatattaat
    12481 atccctgatg gatatggaga cattaaggat atagtgtttt cggctcatat tatagttagg
    12541 tataataatg gacattgtgg aggtatagat ccgcatataa ttggactttg t
//
LOCUS       DAVHEW010000053        12494 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVHEW010000053 DAVHEW010000000
VERSION     DAVHEW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.053"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..138
                     /locus_tag="VVL53_001375"
     CDS             <1..138
                     /locus_tag="VVL53_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4183536.1"
                     /translation="RSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLSAGIINHELG
                     F"
     gene            329..2620
                     /gene="pcrA"
                     /locus_tag="VVL53_001376"
     CDS             329..2620
                     /gene="pcrA"
                     /locus_tag="VVL53_001376"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU4183537.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2663..3343
                     /gene="radC"
                     /locus_tag="VVL53_001377"
     CDS             2663..3343
                     /gene="radC"
                     /locus_tag="VVL53_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU4183538.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3340..4029)
                     /locus_tag="VVL53_001378"
     CDS             complement(3340..4029)
                     /locus_tag="VVL53_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4183539.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4047..4688)
                     /locus_tag="VVL53_001379"
     CDS             complement(4047..4688)
                     /locus_tag="VVL53_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU4183540.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4771..4986)
                     /locus_tag="VVL53_001380"
     CDS             complement(4771..4986)
                     /locus_tag="VVL53_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU4183541.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4994..5341)
                     /locus_tag="VVL53_001381"
     CDS             complement(4994..5341)
                     /locus_tag="VVL53_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU4183542.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5346..6461)
                     /locus_tag="VVL53_001382"
     CDS             complement(5346..6461)
                     /locus_tag="VVL53_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4183543.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6471..7430)
                     /locus_tag="VVL53_001383"
     CDS             complement(6471..7430)
                     /locus_tag="VVL53_001383"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU4183544.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7543..8112)
                     /locus_tag="VVL53_001384"
     CDS             complement(7543..8112)
                     /locus_tag="VVL53_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU4183545.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8240..8911
                     /locus_tag="VVL53_001385"
     CDS             8240..8911
                     /locus_tag="VVL53_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU4183546.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8895..9713
                     /locus_tag="VVL53_001386"
     CDS             8895..9713
                     /locus_tag="VVL53_001386"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU4183547.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9710..10606
                     /locus_tag="VVL53_001387"
     CDS             9710..10606
                     /locus_tag="VVL53_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4183548.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10650..11624
                     /gene="pta"
                     /locus_tag="VVL53_001388"
     CDS             10650..11624
                     /gene="pta"
                     /locus_tag="VVL53_001388"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU4183549.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(11886..>12494)
                     /locus_tag="VVL53_001389"
     CDS             complement(11886..>12494)
                     /locus_tag="VVL53_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4183550.1"
                     /translation="TVSQAVSLPNAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSH
                     EYKAIKRYWKLIQQDSRKLSDKRFYRPIFRMHLTNKEILNKLLSYSEDLKHHYQLYQL
                     LLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIINALQLHYSNAKLEAT
                     NNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERMKFVLSRA"
BASE COUNT         3650 a         2373 c         2695 g         3776 t
ORIGIN      
        1 agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatatt ttcaacaacc
       61 tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgagtgctgg tattatcaat
      121 catgaactag gattctagtt ttgcaggaag tctattgagg tattgagcta gtttatgaaa
      181 aaattgggtg aaaagtcgag tgttttagaa acccacagtg tagtattcta gtttcaatcc
      241 actatatttt gctactcccc gtaaagtttc tattttccct gatttctgat ataatagaaa
      301 tattgacttc aagagtaagg aagagaagat gaacgcatta ttaaatggaa tgaatgaccg
      361 tcaggctgag gcggtgcaaa cgacagaagg tcccttgcta atcatggcag gggctggttc
      421 tggaaagact cgtgttttga cccaccgtat cgcttatttg attgatgaaa agctggtcaa
      481 tccttggaat atcttggcca ttacctttac caacaaggct gcgcgtgaga tgaaagagcg
      541 tgcttatagc ctcaatccag cgactcagga ctgtctgatt gcgaccttcc actccatgtg
      601 tgtgcgtatt ttgcgtcgcg atgcggacca tattggctac aatcgtaatt ttacaattgt
      661 ggatcctggt gaacagcgaa cgctcatgaa acgtattctc aaacagttga acttggaccc
      721 taaaaaatgg aatgaacgaa ctattttggg gaccatttcc aatgctaaga atgatttgat
      781 tgatgatgtt gcttatgctg cccaagctgg cgatatgtat acgcaaattg tggcccagtg
      841 ttatacagcc tatcaaaaag aacttcgtca gtctgaatcc gttgactttg atgatttgat
      901 tatgctgacc ttgcgtctct ttgatcaaaa tcctgatgtt ttgacctact accagcaaaa
      961 attccaatac atccacgttg atgagtacca agataccaac cacgctcagt accaattggt
     1021 caaactcttg gcttcccgtt ttaaaaatat ctgtgtggtt ggggatgcgg accagtctat
     1081 ctacggttgg cgtggtgctg atatgcagaa tatcttggac tttgaaaagg attaccccaa
     1141 agccaaggtt gttttgttgg aggaaaatta ccgctcaacc aaaaccattc tccaagcggc
     1201 caacgaggtt attaaaaata ataaaaatcg ccgtcctaaa aatctctgga ctcaaaacgc
     1261 tgatggggag caaatcgttt actatcgtgc cgatgatgag ctggatgagg ctgtatttgt
     1321 agccagaacc atcgatgaac ttagtcgcag tcaaaacttc cttcataagg attttgcagt
     1381 tctctatcgg actaatgccc agtcccgtac aattgaggaa gccctgctca agtctaacat
     1441 tccttatacc atggttggcg gaaccaaatt ctacagccgt aaggaaattc gcgatattat
     1501 tgcttatctc aaccttattg ctaatttgag tgacaatatt agttttgagc gtattatcaa
     1561 cgagcctaaa cgtggaattg gtctaggtac agttgagaaa atccgtgatt ttgcaaattt
     1621 gcaaaatatg tctatgctgg atgcttctgc taatattatg ttgtctggta tcaagggtaa
     1681 ggcagcccaa tctatctggg attttgccaa tatgatgctt gatttgcggg agcagctaga
     1741 ccacttaagc attacagagt tggttgagtc cgtcctagaa aaaacaggtt atgtcgatat
     1801 tcttaacgcc caagcgactc tagaaagcaa ggcacgggtt gaaaatatcg aagagtttct
     1861 ttctgttacg aagaactttg atgacaccac ggatgtgaca gaagaggaaa ctggtctgga
     1921 caaactgagt cgtttcttaa atgacttggc tttgattgcc gacacagatt caggtagtca
     1981 ggagacatca gaagtgacct tgatgaccct gcatgctgcc aaaggtctcg aatttccagt
     2041 tgtctttttg attgggatgg aagaaaatgt ctttccactt agtcgtgcga ctgaagattc
     2101 agatgaatta gaagaagagc gccgtctagc ctatgtaggt atcacgcgtg cagagaaaat
     2161 tctctatctg accaatgcca actcacgctt gctttttggt cgtaccaatt ataaccgtcc
     2221 gactcgtttt attaacgaaa tcagttcaga cttgcttgag tatcaaggtc tggctcgtcc
     2281 tgcaaataca agctttaagg catcatatag cagtggtagt atttcctttg gtcaaggtat
     2341 gagtttggct caggctcttc aagaccgtaa acgcggtgct gccccaaaat caatccagtc
     2401 aagcggtctt ccatttggtc aatttacagc tggcgcaaaa ccagcatcta gcgaggcaaa
     2461 ttggtccatt ggtgatattg ctctccacaa gaaatgggga gagggaaccg ttctggaagt
     2521 ttcaggtagc ggtgctaggc aggaattgaa aatcaatttc ccagaagtag gtttgaaaaa
     2581 acttttagcc agtgtggctc caattgagaa aaaaatctaa ttttccatcc ttctcacgaa
     2641 taataaagtg aggaggattt ttatgtacag tatttcattc caagaagatt cactattacc
     2701 aagagaaagg ctggccaagg aaggagttga agcgcttagt aaccaagagt tgctagctat
     2761 tttactcagg acaggaacac gtcaagctag cgtttttgaa attgcccaaa aagtcttgaa
     2821 caatctttca agcctaacgg atttgaaaaa aatgaccctg caggaattgc agagtttgtc
     2881 tggtattggg cgtgttaagg ccatagaatt acaagctatg attgaactgg ggcatcgtat
     2941 tcacaaacac gagactcttg aaatggaaag tattctcagc agtcaaaagt tggccaagaa
     3001 gatgcagcag gaattagggg ataaaaaaca agagcacctg gtggcactct atctcaatac
     3061 tcaaaatcaa atcatccatc agcagaccat ttttatcggg tctgtaactc gtagtatcgc
     3121 tgaaccgcga gagattcttc actatgcaat caagcatatg gcgacttctc ttatcttggt
     3181 ccacaatcat ccttcaggag cggtagcgcc tagccaaaat gatgatcatg tcactaaact
     3241 tgttaaagaa gcctgcgaat tgatggggat tgttctcttg gaccatttga ttgtctctca
     3301 ttctaattac tttagttatc gtgaaaagac agatttaatc taaagttcat taacgacata
     3361 gtcaaagagt tttttatctt tgggacgatt ttcaaaaaga agttctggat gccattggac
     3421 accgagaaag gcgacatcat ccgtactcat gacagcctca atgataccat ctttaggatc
     3481 atgagccaca acttttaaat ttggtgctaa gtccttgatg ctctggtggt ggaaggagtt
     3541 gatatgagag atttctccat agatttcttg gagaacggta tctggttctg ttaccaagcg
     3601 ttgagttgtg tactcaacag aagaatcctg ccaatggtct tcgatatctt ggtacaaagt
     3661 tccacccatg gcaacgttaa agagttgggt accacggcag acagagaaaa tgggcttttt
     3721 ctgtttaata gcttccttga tgagggccag ttcgaagata tctctttgaa ggtgatagtc
     3781 atcactatca atggttttgg gttcgccata aaattttgga tcgacatttt gcccacctgt
     3841 caagatgagc ttgtcaatca aactgatata gtggcaggcc atttcttgat caccaatcgg
     3901 taggatgatg ggaatccctc cagcatcttt aacgccttca acaaagcctt ttgctgcgta
     3961 gctcatcatg atgtcatcat ctggatgagt tttttcgttt cctgtaatcc caataactgg
     4021 ttttttcata aaatgatttt cgctttctaa tcctcttttc gcatgaagta gaggagggtt
     4081 tggagttcac ttgtcaaatc gacatactga acgaccacgt cttttggtaa atgcagatgg
     4141 actggtgaaa aactgagaat tcctttcaca ccagcatcaa ccaagagatt agcaacctct
     4201 tgtgacttga cgctgggaac agttaggata gcagtcttca catcagcatc cttgatttta
     4261 tccttgatct gagaaatccc gtaaatggga atcccgtcag gagtttgggt accgacttca
     4321 ggatggtcgt ctaggtcaaa ggccatgata atcttcatct tgttacgttc gtggaagcgg
     4381 tagtggagaa gggcatggcc catatttcca ataccaacca gcatgacatt ggtaatagag
     4441 ttgtcattga gcaaatcggc aaaaaatgtc attagttttt tgacatcata tccaaaacca
     4501 cgacgaccaa gttcaccaaa ataggaaaaa tcacgacgta cggtcgctga atcaataccg
     4561 atagcctctg caatttgctt agagttggca cgttcaatct tttctgcatg aaatctctta
     4621 aaaattcgat agtagagaga gagtcttttt gctgtagctt ttggaatagc aaactgttta
     4681 tctttcacaa aatcacaacc tttctattct tctattttat agaaacattg tgaaaaaatc
     4741 aacaaaaata agaaaaaact aagaaaaatc ttagttttga tgtaaaaaat ctgcatgaga
     4801 tagaaaacgg tagaggtctc cgaccagccc ctgataaact tttttgcccc taaaagtcag
     4861 agaagtcaca taaagtgtat ctggtaaggt tacacatcct gacaaagtca acatgagagc
     4921 ctcatgatcc tcatacttga gagtacgctc tacatgatag cagtccttat aggtcagttc
     4981 aaacattttg gctctatctt tccgattttg taaagacacc acgttctacc aagctatcca
     5041 tgaggaagta gaatttttcc tgatgaatat ggtggtcttc tgatttgaaa atatcaacta
     5101 gacgaaggcc aaacttgtca gtgatattga ttttagcccc tgtaagttcc ttgttaatga
     5161 tgattttgag ttggaagcct tcaccgctgt ttggcacttt ttccaaaagg cgagtcagtt
     5221 catagttacc aaccttagtt tcaaaaaagg tgttatcttt gagggtgaat tttttaacag
     5281 aagggctaag agtgtaatcg taacgacaat tttttaactg aatgattttt tcaaatgcca
     5341 tatggctaac ctccgataat ttcttttaag gtttttgcga gggtttgtag gtcttcaacg
     5401 gtattttgtg gcgacaaact gatgcgaagg gattccttca agcgttctga atttgcgcca
     5461 tacatggctt taagaacatg gctggattgg acaacgcctg cagtacaggc tgagccagta
     5521 gagattgaaa ttccagctaa atctagccga aggagtaaga ggtcattttt ctgaccagga
     5581 aatccaatat tgagaacata agggagatga tgttttcctc tattcaagta atactgaatg
     5641 ccctccagct ctgccagaaa ggcagtttct agattttgta catgttgaaa atgttcttct
     5701 tgtttttcta ggtcttcttt tagggctgca accatgccta cgatggcagg cagattttca
     5761 gttcctgcac gttttttctg ttcctgatct ccgccatgta gataggaatc aaagtccatg
     5821 ctagatgcgt agagaaaacc gattccctta ggaccatgga atttgtgggc agaagcagtg
     5881 agaaaatcaa tgcccaattc ttctgaatga attgggattt taccaatagc ctgaactgca
     5941 tcaacatgat aggcagcagg gtgttgcttg agtatttggc caatttcagc gatgggaagt
     6001 aggtttcctg tctcattatt ggcaaacata gtagaaacca aaatcgtatc gtcacgtaaa
     6061 gccttttgaa tttgctgggc tgtgatttct tgattttctg gctggataat ggttgcttca
     6121 aacccaaagt gttgaaccaa gtaatcaatt gtttcaagga cagcatggtg ctcgatggca
     6181 gttgtgatga tatgttttcc ttgttcttgg tgacgaagac agtagccaat gatggtagta
     6241 ttattgcctt cagtcccacc agaagtgaaa aagatatgtt gaggttttgt ccttagtaac
     6301 tgggctagtt cctgacgggc ttctcgcaag agtttgccag cttgacgacc atgaccatga
     6361 atactagaag gatttccgtg ggtttcttgc ataaccttgg tcatagctga aatagcaact
     6421 gctgacatag gagtcgttgc agcattgtcc aaataaatca aagaatcacc ttatttcttt
     6481 ttattgtagg caaagagtgg gctgactggt tttctttcgt gaatacggac gatagcatca
     6541 ccaattaact cactagcagt gatgtagcat acatttttag gagttttttc ttttgttgct
     6601 actgaatcag tcacaagaat ttctttaata ttagtattgt caagaagctc agcagctccc
     6661 tcgacgaaga gaccgtggct agaaacagca taaatttctg tagctccttc acgttcaacg
     6721 attttagaag cttcagagaa ggtacgtcct gtatttaaaa tatcatcaat caagatagct
     6781 ttcttacctt caacatcacc aataatataa ccttcgttac gagttgcatc gtcttgaggg
     6841 tagtcgataa tggcgatagg agcatcaaga tattcagcca ggctacgcgc acgtttgaca
     6901 cctgaatttt tagggctaac gacaacaaca tctgaaccaa gcaatccttt atcgcagtaa
     6961 tgttttgcga ataggggaac agtgaaaaga ttatccactg gaatatcaaa gaaaccttga
     7021 acctgaacgg catgcaaatc aagagtcagg atacgatcaa ctccagcctt aaccagcata
     7081 ttggcaacta gttttgctgt aagtggctca cgaggacaag caatgcggtc ttgacgtgca
     7141 tagccaaaat atggaaggac aacgttgata ctgtgggcac ttgcacgcac acaagcatcg
     7201 accatgatta acaattccat taggtggttg ttgacaggga aacttgttga ttggatgatg
     7261 taaacatcat aaccacggac actttcttcg atatttactt ggatttctcc gtctgaaaat
     7321 tgacgtgatg atagttttcc aagtgggaca ccaacagctt gggcaatttt ttgtgcaatc
     7381 tcttggttag agttgagtgc gaaaagtttc atgttttttc tatctgacat tatagaccgt
     7441 cctctgtaaa ctttataaat cctagttata tttaccttac atatatgaac tgggatttgt
     7501 gtatttttat cttttctatt ttaccaaaaa atggagatta tttcagctat ttttcatact
     7561 tttgacaaat cgaaccaatt ttgaaggagc tttttggtag gaaatctgat ttttctctaa
     7621 aaattgtcgg aaatcctgtt tgccttgctc atgattttcc acttcaagct ccaattcgta
     7681 atctgtcata tcaaagtatc ggctctgatc cagtgccatg agaccaatag ctgttttcat
     7741 ttcatagcga agcgttgtta gacaaccaag aacctgccag ttcttacttt ggataccatg
     7801 tttcgccaat tcatccagta ctagcccttg aggaagttct tccttactca gatagttctc
     7861 agcatctttt agttgcaatt tttggttgta ttccatgttt ccaacactct gcgggacttt
     7921 gagtgtcaac tcagcccagt cttcaaaggt tcgaatgcgc atagcgactt tcttttctcg
     7981 cagttcaaaa tcaggcgtgt cgatgtagta atttgtttga agaacaggag tgacacctgt
     8041 gaactggtct tttagacgat tgtattcatc ttttttcaat agtgttttca attcaatttc
     8101 taaatgtttc atttttctta ccttttttta tcgttgaaag cggatttatg gtataataag
     8161 cattgtattt attgtatatg aatctggaga aaaaatcaaa gatatttttg acggataata
     8221 tgagaacaag ggagaatata tgaccttaga atgggaagaa tttctagatc cttacattca
     8281 agctgttggt gagttaaaga ttaaacttcg tggtattcgt aagcaatatc gtaagcaaaa
     8341 taagcattct ccaattgagt ttgtgaccgg tcgagtcaag ccaattgaga gcatcaaaga
     8401 aaaaatggct cgtcgtggca ttacttatgc gaccttggaa cacgatttgc aggatattgc
     8461 tggcttacgt gtgatggttc agtttgtaga tgacgtcaag gaagtagtgg atattttgca
     8521 caagcgtcag gatatgcgaa tcatacagga gcgagattac attactcata gaaaagcatc
     8581 aggctatcgt tcctatcatg tggtagtaga atatacggtt gataccatca atggagctaa
     8641 gactattttg gcagaaattc aaattcgtac tttggccatg aatttctggg caacgataga
     8701 acattctctc aactacaagt accaagggga tttcccagat gagattaaga agcgactgga
     8761 aattacagct agaatcgccc atcagttgga tgaagaaatg ggtgaaattc gtgatgatat
     8821 ccaagaagcc caggcacttt ttgatccttt gagtagaaaa ttaaatgacg gtgtaggaaa
     8881 cagtgacgat acagatgaag aatacaggta aacgaattga tctgatagcc aatagaaaac
     8941 cgcagagtca aagggttttg tatgaattgc gagatcgttt gaagagaaat cagtttatac
     9001 tcaatgatac caatccggat attgtcattt ccattggcgg ggatggtatg ctcttgtcgg
     9061 cctttcataa gtacgaaaat cagcttgaca aggtccgctt tatcggtctt catactggac
     9121 atttgggctt ctatacagat tatcgtgatt ttgagttgga caagctagtg actaatttgc
     9181 agctagatac tggggcaagg gtttcttacc ctgttctgaa tgtgaaggtc tttcttgaaa
     9241 atggtgaagt taagattttc agagcactca acgaagccag catccgcagg tctgatcgaa
     9301 ccatggtggc agatattgta ataaatggtg ttccctttga acgttttcgt ggagacgggc
     9361 taacagtttc gacaccgact ggtagtactg cctataacaa gtctcttggc ggtgctgttt
     9421 tacaccctac cattgaagct ttgcaattaa cggaaattgc cagccttaat aatcgtgttt
     9481 atcgaacact gggctcttcc attattgtgc ctaagaagga taagattgaa cttattccaa
     9541 caagaaacga ttatcatact atttcggttg acaatagcgt ttattctttc cgtaatattg
     9601 agcgtattga gtatcaaatc gaccatcata agattcactt tgtcgcgact cctagccata
     9661 ccagtttctg gaaccgtgtt aaggatgcct ttatcggtga ggtggatgaa tgaggtttga
     9721 atttatcgca gatgaacatg tcaaggttaa gaccttttta aaaaagcacg aggtttctaa
     9781 gggattgctg gccaagatta agtttcgagg tggagctatt ctggtcaata atcaaccgca
     9841 aaatgcaacg tatctattgg acgttggaga ctacgttacc atttacattc ccgctgagga
     9901 aggctttgaa accttggagg ctattgagca tccattagat attctctatg aggatgacca
     9961 ctttctagtc ttgaataaac cctatggagt ggcttctatt cctagtgtca atcactctaa
    10021 taccattgcc aattttatca agggttacta tgtcaagcaa aattatgaaa atcagcaggt
    10081 tcacattgtt accagactag atagggacac ttctggcttg atgctctttg ccaagcacgg
    10141 ttatgcccat gcacgattag acaagcagtt gcagaagaaa tctatcgaga aacgctactt
    10201 tgctttggtt aagggagatg gacatttgga gccagaaggg gaaattattg ctccgattgc
    10261 gcgtgatgaa gattccatta ttaccagacg agtggctaaa ggcggaaagt atgcccatac
    10321 ttcatacaag attgtagctt cttatggaaa tattcacttg gtctatattc acctgcacac
    10381 tggtcgaacc catcaaatcc gagtccattt ttctcatatc ggttttcctt tgctgggaga
    10441 tgatttgtat ggtggtagtc tggaagatgg tattcaacgt caggctctgc attgccatta
    10501 cctatccttt tatcatccat ttttagagca agacttgcag ttagaaagtc ccttgccgga
    10561 tgatttcagt aaccttatta cccagttatc aactaatact ctataaaaac tgtctcagag
    10621 tataattatt atcttaaagg agaaaactca tggaagtttt tgaaagtctc aaagccaacc
    10681 ttgttggtaa aaatgctcgt atcgttctcc ctgaagggga agagcctcgt attcttcaag
    10741 caacaaaacg cttagtaaaa gaaacagaag tgattcctgt tttgcttgga aatcctgaaa
    10801 aaattaaaat ttatcttgaa attgaaggaa tcatggatgg ttatgaggtc atcgaccctc
    10861 aacattatcc tcaatttgaa gaaatggttt ctgccttggt ggagcgtcgc aagggcaaaa
    10921 tgactgaaga agatgtacgc aaggttttgg ttgaagatgt caactacttt ggtgtgatgt
    10981 tggtttactt gggcttggtt gatggaatgg tgtcaggagc gattcactca acagcttcaa
    11041 cagttcgccc agctctacaa atcatcaaaa ctcgtccaaa tgtaactcgt acttcaggag
    11101 ccttcctcat ggttcgtggt acggaacgtt acctatttgg agactgtgcc attaatatca
    11161 atccagatgc agaagccttg gctgaaattg ccatcaactc agcaatcaca gctaagatgt
    11221 ttggcatcga acctaaaatt gccatgttga gctattctac taaaggttca gggtttggtg
    11281 aaagcgttga taaggtcgtt gaagcaacta aaattgctca cgacttgcgt cctgaccttg
    11341 aaatcgatgg tgagttgcaa tttgatgcgg cctttgttcc cgaaactgca gctctgaaag
    11401 ctccgggaag tacagtagct ggtcaagcaa atgtcttcat cttcccaggt atcgaggcag
    11461 gaaatatcgg ttacaagatg gctgaacgcc tgggtggctt tgcggctgta ggacctgttt
    11521 tgcaaggttt aaacaagcca gttaatgatc tttctcgtgg atgtaatgca gatgatgttt
    11581 acaagttgac cctcatcaca gcagctcaag cagttcatca atagtgaaaa ctataaagtg
    11641 atatactatg ctatactgta gttatgaaac tatgtacgaa aagcactgcc attaattcct
    11701 gagaactaaa ttactgattg gtgtcaaaaa ggaaaacttc caagcgatga tatcctgtct
    11761 atacacgacc tatagaaatc tgtaatatac atatccgtaa aacgataaat tccctttttg
    11821 attttaaatg agtatgaaaa gagaattttt tggctctttg tcaactgtag tgggttgaag
    11881 aaaagctaag ctcgagaaag gacaaatttc atcctttctt ttttgatatt cagagcgata
    11941 aaaatccgtt ttttgaagtt ttcaaagttc cgaaaaccaa aggcattgcg cttgataagt
    12001 ttgatgagat tattggtcgc ttccagtttg gcgttagaat agtgtagttg aagggcgttg
    12061 ataatctttt ctttatcttt gaggaaggtt ttaaagacag tctgaaaaat aggatgaacc
    12121 tgcttaagat tgtcctcaat aagtccgaaa aatttctctg gttccttatt ctggaagtga
    12181 aaaagcaaga gttgatagag ctgatagtgg tgtttcaagt cttccgaata gctcaaaagc
    12241 ttgtttaaaa tctctttatt ggttaagtgc atacgaaaaa taggacgata aaatcgctta
    12301 tcactcagtt tacggctatc ctgttgaatg agtttccagt agcgcttgat agccttgtat
    12361 tcatgggatt ttcgatgaaa ctgattcatg atttggacac gcacacgact catagcacgg
    12421 ctaagatgtt gtacaatgtg aaagcgatca agaacgattt tagcattcgg gagtgaaaca
    12481 gcctgggaga ctgt
//
LOCUS       DAVHEW010000054        12359 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVHEW010000054 DAVHEW010000000
VERSION     DAVHEW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.054"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            95..1303
                     /locus_tag="VVL53_001390"
     CDS             95..1303
                     /locus_tag="VVL53_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4183551.1"
                     /translation="MNYIKRPHYLDFLRKHRDRPIIKVVSGVRRAGKSVLFQLYKEEL
                     LATGVDEDQIIFINFEDLSYYDLRHFQTLFAYIKDQLVSKKTYYIFLDEIQYVEKFEL
                     VADSLFILANVDLYLTGSNAYFMSSQLATNLTGRYVEIEVLPLSFEEYLSGQSLTENL
                     NTTEIFNNYLFSAFPYLLQTSSYDEKIDYLRGIYNSILLNDIVTRLGKPNPTIIERIV
                     RTLLSSTGSLISTNKIRNTLVSQNVSISHNTLENYLTTLTDSLLFYSVPRFDVKGRAL
                     LQRLEKYYPVDLGLRHLLLPDQKEDIRHILENMVYLELRRRYSQVYVGNLDKYEVDFV
                     VVTDLGHYAYYQVSETTLAPETLERELRPLEAIKDQFPKYLLTMDTIQPTANYNGIEK
                     KSIIDWLLEK"
     gene            1679..2083
                     /locus_tag="VVL53_001391"
     CDS             1679..2083
                     /locus_tag="VVL53_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4183552.1"
                     /translation="MLFFSISNDLLQLLTSWEKQQRYELAKFGILSNPEQFVFTYIDT
                     KGNINKPLHADYLNNKMKSIRKRHKELTHATPHKLRHTGATLAKKAGMSLEAISEALT
                     HSDTVTTKTYVNTSNIVPLSAGQVAYQHLKNK"
     gene            complement(2346..2738)
                     /gene="rpsI"
                     /locus_tag="VVL53_001392"
     CDS             complement(2346..2738)
                     /gene="rpsI"
                     /locus_tag="VVL53_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU4183553.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(2757..3203)
                     /gene="rplM"
                     /locus_tag="VVL53_001393"
     CDS             complement(2757..3203)
                     /gene="rplM"
                     /locus_tag="VVL53_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU4183554.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            3559..3876
                     /locus_tag="VVL53_001394"
     CDS             3559..3876
                     /locus_tag="VVL53_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU4183555.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(4026..4838)
                     /gene="folK"
                     /locus_tag="VVL53_001395"
     CDS             complement(4026..4838)
                     /gene="folK"
                     /locus_tag="VVL53_001395"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU4183556.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFETYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFTNQVVEVETWLPAQVLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(4881..5435)
                     /gene="folE"
                     /locus_tag="VVL53_001396"
     CDS             complement(4881..5435)
                     /gene="folE"
                     /locus_tag="VVL53_001396"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU4183557.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(5416..6738)
                     /locus_tag="VVL53_001397"
     CDS             complement(5416..6738)
                     /locus_tag="VVL53_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU4183558.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTIRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(6740..7714)
                     /gene="folP"
                     /locus_tag="VVL53_001398"
     CDS             complement(6740..7714)
                     /gene="folP"
                     /locus_tag="VVL53_001398"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU4183559.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFNQTFTVEELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(7809..8516)
                     /locus_tag="VVL53_001399"
     CDS             complement(7809..8516)
                     /locus_tag="VVL53_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183560.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(8569..9987)
                     /locus_tag="VVL53_001400"
     CDS             complement(8569..9987)
                     /locus_tag="VVL53_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU4183561.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            10177..10989
                     /locus_tag="VVL53_001401"
     CDS             10177..10989
                     /locus_tag="VVL53_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4183562.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYSLAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            11329..12348
                     /gene="adhP"
                     /locus_tag="VVL53_001402"
     CDS             11329..12348
                     /gene="adhP"
                     /locus_tag="VVL53_001402"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU4183563.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3794 a         2661 c         2344 g         3560 t
ORIGIN      
        1 taaacactta gaaataatca aacaaattaa atattatttc taaatatttg aaaataacat
       61 ctatttgtat tatactatct ttgaggtaac tattatgaac tatatcaaaa gaccacatta
      121 tttagatttt ttaagaaaac atcgtgaccg accaatcatc aaagttgtga gtggagttag
      181 acgagctggt aaatctgtgc tttttcaact ctataaagag gagttactag caactggggt
      241 agacgaggat cagattatat tcatcaattt cgaagatttg agttactatg atctgcgaca
      301 ttttcaaaca ttattcgctt atataaaaga tcaattagtt agcaagaaaa catactatat
      361 ctttttagat gaaattcaat atgttgaaaa atttgaactg gtagcagata gtctattcat
      421 cttagcaaat gtagacctct atttgactgg atctaacgcc tactttatga gtagccaatt
      481 agcaacaaac ttgactggtc ggtatgttga gatagaggtt cttcctttgt catttgaaga
      541 atatctatca ggtcaatctc tcacagagaa tctgaataca acagaaattt ttaacaatta
      601 tctctttagt gctttccctt acttattgca aacatcatct tacgatgaaa aaattgacta
      661 tctcagagga atatataact ccatactgtt aaatgatatt gtcactagat tgggaaaacc
      721 aaatcctact attattgagc gcattgtccg aacccttctc agtagtacag gtagcttaat
      781 atcaacaaat aagattcgca ataccctagt cagccaaaat gtttcaatat cccataatac
      841 tttggaaaat tatttgacaa ctttgacaga tagtttactt ttttattccg ttccacgttt
      901 tgatgtaaaa ggtagagcat tattgcaacg tttagaaaaa tattatcccg ttgatttagg
      961 tttacgacat ctcttattac cagaccagaa agaagacatt aggcatatct tggaaaatat
     1021 ggtatatttg gaattgagac gtagatattc acaagtatat gttggtaatt tagataagta
     1081 tgaggttgat tttgttgttg taactgatct tggccactac gcttattatc aggtcagtga
     1141 aacaacactt gctccagaaa cactagaaag agaacttaga ccactagaag ccattaaaga
     1201 tcaattccct aaatatctat taacaatgga tacgattcag ccaacagcca attacaatgg
     1261 aatcgagaag aaaagcatta tagattggtt actagaaaaa tagataaata taaatcatac
     1321 agctaattag atttgcaaca gtctgttatc aatgattcta cccaaatcct aacaagatat
     1381 agtgaatttc gaatacgcta tataatacgg acacttgaaa atagaaattg gggatgaaag
     1441 gggatctata atttctggaa gtactatcaa aaattaatat catagtctta ttagagaata
     1501 gcatcaccca ctttctcaaa taagattaaa ttgtaactga attataatga aaaagagact
     1561 gagcaatcag tctttaaaat cagaaaagcg catagtatca ggtattgaac aaccttgata
     1621 atatgcgttt tattatggaa atatttgctt cattttctcc tgaaatagag cttttgctat
     1681 cctatttttc tctatttcta atgatttact tcaacttctt acctcttggg aaaaacaaca
     1741 aagatatgaa ctagcaaagt ttggcatcct cagtaatcca gaacaatttg tttttacata
     1801 tatcgacaca aaaggaaaca tcaacaagcc tttacatgcc gactatctga acaataaaat
     1861 gaaaagcatt agaaagcgac ataaagaact gacacacgct acaccgcata aattacgaca
     1921 tacaggagcg acacttgcta aaaaagcagg aatgagctta gaagccattt ctgaagcatt
     1981 aactcatagc gataccgtta caactaagac ttatgttaat acttcaaaca tagttccatt
     2041 atctgctgga caagttgcct accaacatct taaaaataag tagggtaatt ttagggcaaa
     2101 ttttagggta aactttttta gaaagtatag aaaaaagcac ccatagggta aacccttgag
     2161 tgctttgaaa cgttgatata atcgtattga ttaacgtttt gaggcaactg accatgtcag
     2221 gttgcagcca catttgtaag cgactaaagt cgcaacaact gtgtcaattg caccaattta
     2281 gtaagaaagt ataaaaaaag aacaccccga aaggtgctct gtataagtat agtaattctt
     2341 tcgaattaac gtttactaaa ttgtgatgct ttacgagctt tcttaagacc tggtttctta
     2401 cgttcaactt tacgtgagtc acgtgtaaga agtcctgcgc gtttcaatga atcgcggaag
     2461 tctgggtcta cttgaagaag ggcacgagcg ataccgtgac ggatagctcc tgattgacca
     2521 gcgtatccac cacctataac gttaacgaaa acgtcgtatg aacctacagt tgaagtaact
     2581 gcgaatggtt ggttgatgac aagacgaagg tcagcgtgtg ggatgtactc ttcaacatct
     2641 tttttgttaa cagtgatttt accagttcct ggaacaaggc gaacgcgtgc aacagcgttt
     2701 ttacgacgtc cagtacctgc atattgtgct tgtgacatac tttattgttc ctttccttag
     2761 ataagtcctg aaatgtcaag aacttctggt tgttgtgcag cgtgagtgtg ctcagctcca
     2821 acaaatactt tcaacttcat accttgagcg cgtccaagtg tattgtgtgg aagcatacct
     2881 ttaactgatt tctcaatcaa acgtactgca tttttagaac gaagttcacc tgcagagatt
     2941 tgtttcaatc cacctgggtg gtttgagtga gtgtagtaga ttttatcagt tgctttttta
     3001 ccagtcaatt taactttttc agcattgata acaatcacga agtcacctgt atcagtgtgt
     3061 ggtgtaaatg ttggtttgtt ttttccgcga agtacgctag caactactgc agaaagacgt
     3121 ccaagtggta catcagttgc gtcaactacg taccatttac gttcaacttg gcctggttta
     3181 gccataaatg ttgttttgtt catgatttct cctatatata gttcgatttt tgtttacggt
     3241 gatgggtgtt ccttcccatc gaaaagtatt tggaaggttc cggggccttt caaatggggt
     3301 aaacaatacc gcctactatc ataccaaatt ttacccctaa aagtcaatag atatgaaaaa
     3361 tatttttcgg taacttctca aagtaactga aagttatata atcaagttaa tttgggaatt
     3421 tacctattaa cttcggtaga tttttaggaa ttttttccct atttctccct gtaactaaaa
     3481 tccttttcaa aaaatcacct tttcttgatt tttaatccta tttgctgtat gataagggaa
     3541 aagaaagggg acagagatat ggcttttacc aatacccaca tgcgatctgc tagttttggt
     3601 attgttacca gcttgcctga tgacatcatt gactcttttt ggtatatcat cgaccatttc
     3661 ttaaaaaatg tctttgaatt ggaagaagaa ctcgagtttc aattgcttaa taaccaagga
     3721 aagattacct tccacttttc aagtcaacac ctccctacag ccattgattt tgactttaac
     3781 catcctttcg accctcgtta tcccccaaga gtactggttt tagacatgga cggtagagaa
     3841 actatcctcc tcccagaaga aaatgaccta ttttaatact caatgaaaat caaagagcaa
     3901 actaggaagc tagccgcaag ctgtacttga gtacggtaag gcgacgctga cgtggtttga
     3961 agagattttc gaagagtata aaaactctag ccttcagttg caagtaactg aaaactagag
     4021 tttttctatt ttttcaaagc atcatacaag ttgcggatcg gttgttttaa tatcggatgg
     4081 ataaaatgag gcgcaatttc ctgtaaggac tcaaggacaa aaaggcgttc cgctatgtaa
     4141 ggatgaggca atatgaggtc gtctgtataa aggatctggt cctccacaaa gagcaagtcc
     4201 aaatcaatca aacgagggcc ccaatgcact tctctcaccc gtcccagctc tgactcaatg
     4261 gctaacaagg tttctaatag cacttgggca ggcaaccatg tttctacttc aaccacttga
     4321 ttggtaaagc tatcctgctc aacaccaccc caaggctccg tcgctaagac actggactct
     4381 ttgagaatat ggatgccacg agctcgcagt ttgtcaatgg cttgcttcaa gtttgcttgt
     4441 ttatctccca tattgcttcc tagggcgata aaggctcgtt gcttgcggcg atgaatggtt
     4501 accgagcaag tatctagtga caaatgcacc ggtgcccaag gttttttcag ttccaacttc
     4561 atttcttgga caagaggata ggtctcaaag gtacgttcca ccagtttata ggctaccgtt
     4621 tcaatcaagt cttcactcgt ttcctgaaac caagtcgtcc actgctgaca caattctccg
     4681 taatggacag aggctgttaa atccaagtct gtagctgcct tggtcatatc ataggatagg
     4741 atggctgaaa cgacaaattt ctgccccaat tctttctcac taggaaaaag accatgatag
     4801 gcaaacattt ccaaatcctt aatctgcagt tgatccataa tgattccttt ctagaaaaat
     4861 ccgcttgaag cggattcttt ttatagcccc attaaacgat aagcttggtc gcggaggtcc
     4921 ttatctgttt caaatagacc acgagctact gtcgtcaagg ttgcagtgcc tggttttcta
     4981 acaccacgca tactcataca catatgttcc gcctcaatga caacaaaggc tcctttagca
     5041 cctagatagt ccatcaaggc atcggccact tcgatattca aacgttcttg aatttgtggt
     5101 tttttcgaat aaacttcaac cgtacgggct agcttagaca agcccgccac acgaccatct
     5161 ggaatgtagg caatgtgcgc tctaccataa aatggcaaga agtggtgttc acacatggta
     5221 tggaaaaaga tatccttttc taccaccata ttatcgtcaa taatttcaaa ggattttgac
     5281 aaatgttcct ctgctgtttg accaagacct gaaaaaatct cttgatacat acgggctaca
     5341 cgagcaggtg tttcctgcaa gccctcgcga ttagcgtcct ctcctacagc ctcgataatc
     5401 atttttacag ctgcttcaat cttttgtgta tccattctcg ttcttcctct ccatcagata
     5461 ggccctcact tggctcaaga aatacaagga acctgtgaca atcctaattg tttgtttctc
     5521 ttctttttta tctgtcaaat tctgctctag aaaatcatgc caatcttggt agctgagatt
     5581 tctagactta gctgcctctt tcagcacgct ttcatccgtc gcccgactat ccgcaaaatg
     5641 tgttagagta agctcggtat ctggcatggc tcccagcaag tccaacatat cctccaaggc
     5701 cttggttttg atacaagtga agaggatttc cttatgataa tccgcaaaac gttcttgcaa
     5761 ggttaccaac aaggccttga tagcatgggg attgtgggct ccatccaaaa tcatcaaggg
     5821 atctcttgac acgatttcca aacgccctgg ccaacttgtt tcttccaagg cttgaccaag
     5881 aaagtcattg cttgctagct ctcgaccatc ttcttgacaa aaagtatcaa gtaaagctat
     5941 ggccatccca gcattctcta tttggtacaa accaagcagg ctagtctgga agcgaccttg
     6001 tctgacagca cttgtatagt caaagacttc ccctgtcacc acactttctt gatgacgaac
     6061 ctgataatct gtcccgtagg caagtctcgg cgcatctttc ccttccgcaa tgcggtcaat
     6121 cacagccaag gcttctggag caatacgccc tgttaccaag ggcatccctt gtttgataat
     6181 acctgccttc tgctctgcta tggcctccaa agtgtcacca agtaaggcca catgatccaa
     6241 gccaatagtt gtaattcctg tcaaaatggg ctgacagaca ttggtactat caaaaagtcc
     6301 acccatgcca acttccatga tggccacatc tacttgctct gaggcaaagt agtcataggc
     6361 cagggctgtg ataatctcaa actcggttgt gccctgtaaa ttggcgaccg cttctccctc
     6421 cagcaaagac tgatagtctg ccatgagagc ttctagcctc gcttctgaga tcgattcccc
     6481 attgatgcta atctggtctg tgtaatgaat gagatagggc gagctaaaca cgccaactct
     6541 cagccctagc ttttctagca tctttttcaa aaaagcaata gtcgagccct tgccgttagt
     6601 tcctccgata tggaggacct tgagtttgag atggggattg ccacgcaaag ctaacagttc
     6661 caccattcgt tccaagccaa aatgcggttg atccgtccgg tagttagcaa tccactgatt
     6721 gttttcaatt tctttcatct tatttatatt gttttaaatc taaattttcc gcttcatcag
     6781 ccagacgaat ggcagaggca atttcaactg ccatcctgtg actagctacg tcatgcacgc
     6841 gcaccacttc tacaccctgt ctcgcagcga tactagttac atgagccgaa gccgtgtccc
     6901 gatttcggaa accaagctct gtctcaggat tgacttcaaa cccactttct tcaaggatat
     6961 tgatgacaaa tcgcttgcgc gacactccga gaaagattgg atagcccttc tgatgtagtt
     7021 tatccagatc ccgtaaaagg aggagatttt ctttcttggt cagaccaaag ccaattcctg
     7081 gatccaacag gatattttct ggtgcaatac cagcttctgc cgctctcgct agtgctcgtt
     7141 caaagaaagc caccatcaag tcttcgattg gcaatgtttc aaagtcagct aactcttcta
     7201 ctgtaaaagt ttgattaaat ccaaaatgag ggaatatgag cgagctaggg tgttgaggtc
     7261 gcgccataac tggattaaac atgatgacca cttgcgctct cgcttcagct accacatgag
     7321 ccattttttc atcgcccatg agaccagtga tatcattaac tagattggca ccagcagcca
     7381 aagcagcctc tgctacttga ctcttccaag tatcaataga gatgaggaca tcactttcct
     7441 tgcgaatcgc tttgatcact ggaacaacac gctggatttc ctcttctatc tcaacatagc
     7501 tacttcccgg ccgagtcgat tctccgccga tatctagcat actggctcct tctgctatca
     7561 atttacgagc ctgctggagc gcctgctcaa gagcaaaaaa ttgaccaccg tccgaaaagg
     7621 agtctggggt tacattgata attccgcaaa taactgtctt tgcatgattg gctttacttg
     7681 acatatcggt cactccctca aggattttca tcatattatt tttctatttt accataaaaa
     7741 gaaaaagatg gacacgattg cattcatctt tccctggtag aaagaggtcg gttcctacat
     7801 aaaaactttt aaacagaaat tcctaatgtc cgactcataa tcaccacaag agccaacaaa
     7861 cagaaagcaa tcccattaac aatcatgtga agcaagatcg acatttccaa acgttgggtc
     7921 ttgtaggctg tccaagatag aactgtcgac atacctccat aaatcaataa agaaggtaaa
     7981 ttacttggtt gatgcaataa agcaaacaca atcgtaccga ctacaaatcc caagttctcc
     8041 ttgcctcgga aaatcttttt aggaacaatc ccacgacaca agatttcctc acaaatcgga
     8101 gcaagcaagg ctagcaagaa gaaactggaa atcaacgaac tattttgaac catatcatta
     8161 atctgagact ggttagctgt tgtcgtctca tttgacagtt gcaataaaat ggaaccaagt
     8221 atatttgacc cgacaataac tagataactc aagcccaaac gtgccaaatc tttagctcta
     8281 aaaaaagaaa aattaaaact agctaacttg gttttacgag ctcccataat aaatagagcc
     8341 agaaccacaa ttgaaaggcc agcaactatc agccctgact gtagcagggc tacttctttt
     8401 aaagtcaaaa tagaggtaac cactagggga acctgatata aaaggacggc aagtaaaaag
     8461 accaaaatcc agcctgcacg attcaacaat tctttccaca tatttttctc tttcatcacc
     8521 tatctccttt gtattaaaaa aggggggaaa tccccctggg tcattctatt ataaggccat
     8581 gctgatgtag ttaaggataa acaaggcatc caaaatccaa atcatgacat gaacatcttt
     8641 aacttgacct ttaacaagct tagtcaaagt gtaagtcaag aaaccaactg caatcccttg
     8701 agtgatagag tagctgaatc ccataaagat agatgtgaag aaggcaggaa ctgcttcaga
     8761 catatcatcc caatggatat ttttcaagct accaagcatc ataatcccaa cgataatcaa
     8821 gattggagct gtagccgctg ttggtacgat cgctagaagt gggctaaaga agcttgaaat
     8881 cgcaaaacag atagctacaa ccaaggctgt caaaccagta cgtccacctg caccgatacc
     8941 agcagcagac tcaacataag tcgttacgtt tgaagtacct gcaatggcac cgactgtcgt
     9001 tccaatcaaa tcagagtaaa gagccttatc caatttggct gattggtgat tttcaccatt
     9061 tgtcgctacg ataccaactt tttcacctgt accgatcaag gtaccaattg tgtcaaaaat
     9121 atctgtcaat gagaaggcaa gaatggccat cagagtttca ggcaagcgag ctgtatctga
     9181 aaccaaagct cccaatcctt ctgaaccaag agctgcacca aagattgtct tcaaatcttc
     9241 aaaagctgca ccaacatggt tattagcaaa atcgatacta gacaaatcaa ccaaaccaac
     9301 tgcaatagca agaacagttg ttgtcaagat agagagaata attccccctt taatcccttt
     9361 gatgacaaag aagatagtaa tggcaagtcc tgcaagagcc actaaaacag ctggattatt
     9421 aaagctgacc aatcctggaa ctgctgaaga gtttgctgca atcgttgctt gagctttgtc
     9481 agccccttct cctacaacag tatagttgcc tggatcaatc gtgaatttca aaagtccagc
     9541 attcttaatc cctacatagg caaggaagac accgatacca gctgaaatag ctgagcgaag
     9601 agcattggga atcgattcaa tgatcatttt acgaacattt gtcaaggtaa taatcaatga
     9661 aataatccca cagatgaaga ccatagctag ggcttcttgc caagaataac caagcccgaa
     9721 tacaactgta aaggtaaaga aggcattgag tcccatacct ggcgcttggg cataaggtaa
     9781 gttagcataa aaagccatca tcaaggtacc cgctactgca ccaataatcg tcgctaggaa
     9841 gacgccctga gcaggcattc ctgtttgtga aagtatttgt gggtttacaa agagaatata
     9901 gctcattgca aagaaagttg ttaaaccagc gagaacctct gtacgaacgt ctgtaccgtt
     9961 ctcttttagt ttaaataatt tgtccatcat caatctcctt ttaattttta cgaacaataa
    10021 tataattata tcatttatca ttcacttttt caatgtcttt gtttgatttg tttaagaaat
    10081 taatccaatt tcattttttg tttggaatct gcttttctgc ctgctttctg ttataataga
    10141 agacatctta tctgaaaaca cactagaaag gtccatatga cgaaaaaaat tattgcagtt
    10201 gacctggatg gaaccctgct caactcagac agtcaaattt ctgactttac caaaagaacc
    10261 atcaaaaaag ttgctgaaaa aggccatcag gttattatta cgacaggtcg cccttaccgt
    10321 atgtcaaaag atttttaccg tgaactgggc ttagacactc ctatgattaa cttcaacgga
    10381 tcccttactc atttaccaga ccaagtttgg gattttgaaa agtgtttgac tgtagacaaa
    10441 aaatatctgc tagatatggt tcaacgttca gaggacattc aagccgattt tatcgctgga
    10501 gaatatcgta aaaaattcta cattacaaat cccaatgaag aaattgccaa tcccaaacta
    10561 tttggtgtag aagctttcca gcctgaagat caattccagc ctgaattggt gaccaaggac
    10621 cctaactgta tcctcttgca gactagagcc agtgacaaat attccttggc aaaagaaatg
    10681 aacgccttct accagcatca actttctatc aatacctggg gaggtccgct caatatcctt
    10741 gaatgtaccc caaaaggtgt caacaaggcc tttgctttgg actacttgct caagataatg
    10801 aatcgtgaca aaaaagattt gattgccttt ggagatgaac acaatgatac cgaaatgctc
    10861 gcttttgctg ggaagggtta tgccatgaaa aatgccaatc cagagctact cccttatgca
    10921 gatgagcaaa tttcccttac caacgaccaa gatggggttg ccaaaaccct acaagactta
    10981 ttcttataac ctatactgat actcaatgag aatcaaagag caaacttagg aagctagccg
    11041 taggttgctc aaagcactgc tttgaggttg tagatgaagc tgacgtggtt tgaagagatt
    11101 ttcgaagagt atgaccagct tgcaagctgg tctttgtgct cttttcacag tctcatcaac
    11161 cagttaatcg attgttctac tttttgtctc caaaaccttg gaaaaggctt tcttttgtga
    11221 tatacttcac atataaatac aagagagctc gtcacactcg actctgttga caaaaaatca
    11281 atccagtccc gttgtccctg ttctcggtca atatcaagga ggatagctat gaaagctgtt
    11341 gttgtaaatc cagaaagcac tggtgttgct attgaagaaa aagtactccg tccacttgaa
    11401 actggggaag cacttgtaga agttgaatac tgtggcgttt gccacaccga cctccacgtt
    11461 gctcatggtg actttggtca agtcccagga cgtgttcttg ggcacgaagg tatcggtatc
    11521 gttaaagaga ttgctccaga tgtgaaaagc cttaaagtcg gtgaccgcgt cagcgttgct
    11581 tggttctttg aaggatgtgg cacttgcgaa tactgtacaa ctggtcgcga aaccctttgc
    11641 cgtacagtga aaaatgctgg ctactcagta gacggtggta tggctgaaca atgtatcgta
    11701 actgctgact atgctgtcaa agttcctgac ggacttgatc cagcccaagc ttcttctatc
    11761 acatgtgctg gagtaacaac ctataaagct atcaaagaag caaaagttga accaggccaa
    11821 tgggttgttc tttacggtgc tggtggactt ggtaacctcg ctgttcaata cgctaaaaaa
    11881 gtattcaatg ctcatgttat cgcagtcgat atcaacaatg acaaacttgc ccttgcaaaa
    11941 gaagtaggcg ctgacattgt gattaacggc ctcgaagttg aagatgtagc tggactcatt
    12001 aaagaaaaaa ctgatggagg agctcattca gctgtcgtaa ctgctgtgtc taaagttgcc
    12061 ttcaaccagg ctgttgactc cattcgtgct ggtggtcgcg tcgtcgctgt tggtcttcct
    12121 tctgaaatga tggaactcag catcgttaaa acagtcctcg atggaatcca agtcatcggt
    12181 tctcttgtcg gaactcgtaa agacttagaa gaagccttcc aatttggtgc agaaggtctg
    12241 gtagtcccag ttgttcaaaa acgtccagta gaagatgctg ttgccatttt cgacgaaatg
    12301 gaaaaaggcc aaatccaagg acgtatggta ctcgacttca cccactaatc taatagaaa
//
LOCUS       DAVHEW010000055        12300 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVHEW010000055 DAVHEW010000000
VERSION     DAVHEW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.055"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            153..1382
                     /gene="arcA"
                     /locus_tag="VVL53_001403"
     CDS             153..1382
                     /gene="arcA"
                     /locus_tag="VVL53_001403"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU4183564.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVISKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1440..2456
                     /gene="argF"
                     /locus_tag="VVL53_001404"
     CDS             1440..2456
                     /gene="argF"
                     /locus_tag="VVL53_001404"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU4183565.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2621..3568
                     /gene="arcC"
                     /locus_tag="VVL53_001405"
     CDS             2621..3568
                     /gene="arcC"
                     /locus_tag="VVL53_001405"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU4183566.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3777..5288
                     /locus_tag="VVL53_001406"
     CDS             3777..5288
                     /locus_tag="VVL53_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU4183567.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5310..6641
                     /locus_tag="VVL53_001407"
     CDS             5310..6641
                     /locus_tag="VVL53_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU4183568.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGIKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPEAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            6735..6862
                     /locus_tag="VVL53_001408"
                     /pseudo
     CDS             6735..6862
                     /locus_tag="VVL53_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(7045..7335)
                     /locus_tag="VVL53_001409"
     CDS             complement(7045..7335)
                     /locus_tag="VVL53_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183569.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQTEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQKLMNKAVKDEVAQI"
     gene            complement(7323..7574)
                     /locus_tag="VVL53_001410"
     CDS             complement(7323..7574)
                     /locus_tag="VVL53_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU4183570.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQATE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            7778..7936
                     /locus_tag="VVL53_001411"
     CDS             7778..7936
                     /locus_tag="VVL53_001411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183571.1"
                     /translation="MKIKEQTRKLATGCSKHRFEVVDKTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            complement(7973..8857)
                     /locus_tag="VVL53_001412"
     CDS             complement(7973..8857)
                     /locus_tag="VVL53_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4183572.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(9002..10153)
                     /locus_tag="VVL53_001413"
     CDS             complement(9002..10153)
                     /locus_tag="VVL53_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU4183573.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGDMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10299..12065)
                     /locus_tag="VVL53_001414"
     CDS             complement(10299..12065)
                     /locus_tag="VVL53_001414"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU4183574.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEVSDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYCACQLLGPLHK"
BASE COUNT         3608 a         2566 c         2396 g         3730 t
ORIGIN      
        1 tgtataataa cataaaattg tgctaaatat cataatttat attattttcc gagaaattat
       61 agccattgac attacctagg aattgcgtta taacatacat gtaagcggta cccaaaatgg
      121 tgccaagtca aaatttttaa ggaggaaaat acatgtcttc acatccaatt caggtcttct
      181 cagaaattgg gaaactgaaa aaagttatgt tgcaccgtcc aggcaaggag ttagaaaact
      241 tgttgccgga ctatcttgaa aggcttcttt ttgatgatat tcctttcttg gaagatgctc
      301 aaaaagaaca tgatgcattt gcccaagctc ttcgcgatga aggaattgag gttctctatc
      361 tagaacaact cgctgctgaa tcattgacct ctccagaaat ccgcgatcaa tttatcgagg
      421 aatacttaga cgaagccaac atccgtgatc gtcaaaccaa ggttgctatt cgtgaattgc
      481 ttcacggcat caaggacaac caagaattgg ttgaaaaaac aatggctggg attcaaaaag
      541 ttgaattgcc agaaattcct gacgaagcta aagatctaac tgacttagtt gaatcagagt
      601 atccatttgc aattgacccg atgccaaacc tctatttcac tcgcgaccca tttgcaacaa
      661 ttggaaacgc cgtatcgctt aaccacatgt ttgcagacac tcgtaaccgt gaaacactct
      721 acggtaagta tatcttcaaa taccacccaa tctatggcgg aaaagtggat ttggtctaca
      781 accgtgaaga agatacgcgt atcgaaggtg gagacgagct agttatttct aaagacgtcc
      841 ttgcagtagg tatctctcaa cgtacagacg cagcttctat cgaaaaactt ttggtcaaca
      901 tcttcaagaa aaatgttggc ttcaagaaag ttttggcctt tgaatttgct aacaaccgta
      961 aattcatgca cttggatact gtcttcacta tggtagacta tgacaagttc actattcacc
     1021 cagaaatcga aggcgacctt cacgtttact cagttactta cgaaaacgaa aaacttaaaa
     1081 tcgttgaaga gaaaggtgac ttagctgaac ttcttgctca aaaccttggt gtagaaaaag
     1141 ttcatttgat tcgttgcggt ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg
     1201 acggttctaa cactttgacc atcgcacctg gtgtggtagt tgtttatgac cgcaataccg
     1261 tgaccaataa gattttggaa gaatacgggc ttcgcttgat taagattcgc ggaagtgaat
     1321 tggttcgggg ccgtggtgga cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt
     1381 aatcgctgtt cggtattcgt caatagaaaa tgtaaaaaat agaaagagga aataataaaa
     1441 tgacaaattc agtattccaa ggacgtagct tcttagcaga aaaagacttt acccgtgcag
     1501 agttagaata ccttattggt ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc
     1561 aacaccacta ccttgctggc aagaatatcg ctctcctatt tgaaaaaaca tctactcgta
     1621 ctcgtgcagc ctttacaact gcggctatcg accttggtgc tcacccagaa tacctcggag
     1681 caaatgatat tcagttgggc aaaaaagaat ctactgaaga tactgctaaa gtattgggac
     1741 gtatgtttga cgggattgaa ttccgcggat tcagccaacg tatggttgaa gaattggcag
     1801 aattctcagg cgttccagta tggaacggtc taactgacga atggcaccca actcaaatgc
     1861 tcgctgacta cttgactgtt caagaaaact tcggtcgctt ggaaggcttg acattggtat
     1921 actgtggtga tggacgtaac aacgttgcca acagcttgct cgtaacaggt gctatccttg
     1981 gtgtcaatgt tcacatcttc tcaccaaaag aactcttccc agaaaaagaa atcgttgaat
     2041 tggcagaagg atttgctaaa gaaagtggcg cacatgttct catcactgaa gatgctgatg
     2101 aagcagttaa agatgcagac gttctttaca cagacgtttg ggtatcaatg ggtgaagaag
     2161 acaaattcgc agaacgtgta gctcttctta aaccttacca agtcaatatg gacttagtta
     2221 aaaaagcagg caatgaaaac ttgatcttcc tacactgctt gccagcattc cacgatactc
     2281 acactgttta tggtaaagac gttgctgaaa aatttggtgt agaagaaatg gaagtaacag
     2341 acgaagtctt ccgcagcaag tacgctcgcc acttcgatca agcagaaaac cgtatgcaca
     2401 ctatcaaagc tgttatggct gctacacttg gtaaccttta tattcctaaa gtataatttt
     2461 agataataaa ccgtctacca acagctatga gggctgcgac taatagcttt agtccggtcc
     2521 tcttttatat aatggtaatc tattatttct tataaaatat gtgaaaaatc attaaattga
     2581 aatctaaacg cattctattg agtgtgataa aggagaattt atggcaaatc gtaaaattgt
     2641 agtagctttg ggaggaaatg cgattctttc ttctgaccca tcagcaaagg ctcaacaaga
     2701 agctttagtt gaaacagcta agcatcttgt aaaattgatt aaaaatggag atgatttgat
     2761 tatcactcac ggtaatggac ctcaagttgg gaatctcttg ctccaacatt tggcatcaga
     2821 ctctgaaaag aaccctgcct tcccactcga ctcacttgtc gctatgacag aaggtagcat
     2881 cggtttctgg ttgaaaaatg ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa
     2941 tgttgcctct gttgtaacgc aagttgtcgt agataaaaat gatccagctt ttgttaactt
     3001 gagtaaacca atcggtcctt tctattcaga agaagaagca aaagcagaag ccgaaaagag
     3061 cggagcgact ttcaaggaag atgctggccg tggctggcgt aaggtcgttg cctcaccaaa
     3121 acctgttgac atcaaagaaa ttgaaaccat ccgtactctt ttaaataatg gtcaagtcgt
     3181 cgtagctgca ggtggtggcg gtattcccgt cgtcaaagaa aacaatggac atttgactgg
     3241 tgtcgaagcg gttattgata aagacttcgc ttcccaacgt ttggcagaat tggttgatgc
     3301 agacctcttc atcgttttga caggtgtaga ttatgtattt gttaactaca acaagccaaa
     3361 ccaggaaaaa ttggaacatg tgaatgttgc ccagctggaa gaatatatca aacaagatca
     3421 gtttgcacca ggtagcatgc ttccaaaagt agaagcagct atcgcttttg tcaatggtcg
     3481 tccagaagga aaagcagtta ttacttccct tgaaaatcta ggcgccttga ttgaatctga
     3541 aagcggaaca attattgaaa aaggataagt tgttttacta ataagatgta ttctatttct
     3601 agtatcttta tatcaaatta gaaattattc ttgaaaacat gtacaatatt tcaaaagata
     3661 ctagttttag actttaatat ggtaaaacaa atataaatag aaagcgtttt cttgaatgtt
     3721 tatttaagaa agtagttggt tttttacact ttgttagaca tcaggaggaa aaacaaatga
     3781 gtgaaaaagc taaaaaaggg tttaagatgc cttcatctta caccgtatta ttgataatca
     3841 ttgctattat ggcagtgcta acttggttta tccctgcggg ggcctttata gaaggtattt
     3901 acgaggctca gcctcaaaat ccacaaggga tttgggatgt cctcatggca ccgattcggg
     3961 ctatgctagg tactcatcca gaggaaggtt cgctcattaa agaaacgagc gcagcgattg
     4021 atgtagcctt cttcatcctt atggttggtg gtttccttgg cattgtcaac aaaactggtg
     4081 ctcttgacgt agggattgcc tctatcgtga agaagtataa gggccgcgaa aaaatgttaa
     4141 ttttggtact gatgcctttg tttgccctcg gtggtacaac ttatggtatg ggtgaagaaa
     4201 caatggcctt ctatccactc cttgtgccag ttatgatggc cgttggtttt gatagcctga
     4261 ctggtgttgc aattattttg ctcggttctc aaatcggctg tttggcatct actctgaatc
     4321 catttgcgac aggtattgct tcagcgactg cgggagttgg tacaggggac ggtatcgtac
     4381 ttcgtctgat cttctgggtt accttgactg ctcttagtac ttggtttgtt taccgttatg
     4441 cggataagat tcaaaaagat ccgactaagt cactggttta tagtactcgc aaagaagatt
     4501 tgaaacactt taacgtagaa gaatcttcat ctgtagaatc tacacttagc agcaaacaaa
     4561 aatcagttct cttcttattt gtgttgacat tcatcttgat ggtattgagc ttcattccat
     4621 ggacagacct tggcgttacc atttttgatg actttaatgc ttggttgact ggtcttccag
     4681 ttattggtaa tattgtcggt tcatctactt ctgcactagg tacttggtac ttcccagaag
     4741 gcgccatgct ctttgccttt atgggtatcc tgattggtgt tatttatggt cttaaagaag
     4801 ataagattat ctcttccttc atgaatggtg ctgctgactt gctcagtgtt gccttgatcg
     4861 tagcgattgc tcgtggtatt caagttatca tgaacgacgg tatgattacc gatacaatcc
     4921 tcaactgggg taaagaaggc ttgagcggtc tatcttcaca agtctttatc gttgtaactt
     4981 atatcttcta tctacctatg tcattcttga tcccatcttc atctggtctt gccagcgcaa
     5041 ctatgggtat catggctcca cttggagaat ttgtaaatgt ccgtcctagc ttgattatca
     5101 ctgcttacca atctgcttca ggtgtcttga acttgattgc accaacatct ggtattgtga
     5161 tgggagctct tgcacttgga cgtatcaaca ttggtacttg gtggaaattc atgggcaaac
     5221 tcgtagtcgc tattattgta gtgaccatcg cccttcttct ccttggaacc ttccttccat
     5281 tcctataaaa tagtgagtga ggtgattcca tgaaaataga tataacaaat caagttaaag
     5341 atgaatttct tatatcatta aaaaccttga tttcctatcc ttcagtactc aatgaaggag
     5401 aaaatggaac accttttgga caagcaatcc aagatgtcct agaaaaaact ttagagattt
     5461 gtcgagacat aggtttcact acctatcttg accctaaagg ttattacgga tatgcagaaa
     5521 tcggtcaggg agcagagctt ctggccattc tctgtcattt ggatgttgtt ccatcaggtg
     5581 atgaagcaga ttggcagaca ccgccatttg aagcaactat caaagacggc tgggtattcg
     5641 gacgtggtgt ccaagatgat aaaggccctt cgctcgcagc tctctatgca gtaaaaagct
     5701 tgctggacca aggtattcag ttcaaaaagc gcgtacgctt tatctttggt accgatgagg
     5761 aaaccctctg gcgctgcatg gcacgctaca ataccatcga agaacaggcc agtatgggct
     5821 ttgcacctga ctcatctttt cctctgacct atgctgaaaa agggcttcta caggtcaaac
     5881 ttcatggccc tggatcggat caactagagc ttgaagtagg aggcgccttt aacgttgtac
     5941 cagacaaggc caactaccaa ggtctcctct atgaacaggt ttgtaacggt atcaaagaag
     6001 ctggttatga ttaccaaacc actgaacaaa ccgtaacggt tctcggagtg ccaaagcatg
     6061 ctaaggatgc tagtcaaggt atcaatgctg tcatccgact agctaccatt cttgctcctc
     6121 tccaagaaca ccctgctctc agttttcttg caacacaagc aggtcaagac ggcacaggaa
     6181 gacaaatctt tggtgatata gcagatgaac cttctggtca cctatccttt aatgtcgcag
     6241 gtctcatgat caatcatgaa cgttctgaaa tccgtattga cattcggact cctgtcttag
     6301 ctgacaagga agaactagta aagttgctta caagatgtgc acaaaactac caactccgct
     6361 acgaagagtt tgactatcta gcgcctctat acgtcgcaaa agacagtaaa ctcgttagca
     6421 cactgatgca aatctaccaa gaaaagactg gcgataacag tcctgctatt tcatccggtg
     6481 gtgccacttt tgctcgcacc atgccaaatt gtgtagcctt cggcgcctta ttcccagaag
     6541 cgaagcagac agaacatcag gcaaatgaat gtgccgttct agaagatttg taccgtgcta
     6601 tggatattta tgccgaagcc gtctatcgac ttgcaactta atcaggcaac tgtttctacc
     6661 aaaaaaatcg accgattaat gaactgcacc ccaaaagtta gacagaataa atctaacttt
     6721 tggggtgttt tattatgaaa ttgagttatg aagataaagt tcagatctat gaactaagaa
     6781 agcaaggaca aagcttcaaa cagctttcaa aaagatttat gtggatgttt ctggtctaaa
     6841 gtcatctgaa tctttgagat gagctttata aatcgctttt ttcagttttt gcactggtgt
     6901 ttcgataaac tcaaactttt tagccgtggt attgcctgat tttatagtat attgaaacta
     6961 gaatagtaca cctctccttc taaaacattt ttagaaatcg atttgactgt cctgatcgat
     7021 ttgtcctgtt cttatttcat tttactatat ttgagccact tcgtctttaa cggctttatt
     7081 cataagcttt tgtaattttt ctttactatc aattacttct gattttccgt tgtaatttat
     7141 tgtaataggt tttaacttac ctaatttctc gacacgctca ttaatttgat cttttttgaa
     7201 ggctgcatat gtttttccta agattttttc gaaaatatat ttatcagata gcggtttgcc
     7261 ttcttcttca gtttggtttt tgtattgatt tgaaacataa ggaacaaatc cttcatagta
     7321 acctaatgct cccataagtt caaaagcttg ttttctaatt caaaccattg caactcagat
     7381 ttcagctttt cagataaatc ctgctcatcc aaataatgac ttgaaattag tgctgaactc
     7441 gtttctgtag cctgtacagg ctgagcaccc ataccagcaa aaaataaact cgttcctagc
     7501 aagaccgaac aagctcctat tgcatatttc ctcaaagaaa aacgctgctt tctctcaaat
     7561 tgaaattctt tcatcccatc tcccatcatt cattattact gtatattttg tatatcagaa
     7621 atagtttgta ttcacaaatc tttctagtta ttcccttatc attcctaatt aagggagatg
     7681 acatacaata atttttagtt aaatgtatat cgatgttttt tgtttttctt aataaacgca
     7741 atacaaaaag agcctgttgc caagctcttt gcactcaatg aaaatcaaag agcaaactag
     7801 gaaactagcc acaggttgct caaaacaccg ttttgaggta gtagataaga ctgacgaagt
     7861 cagctcaaaa cactgttttg aggttgcaga tagaactgac gaagtcagta acatctatac
     7921 ggcaaggcga cgctgacgtg gtttgaagag attttcgaag agtattagtc tattatttct
     7981 tctcagcgcg aagggctgac aagatttgtg ttcggatatc atccacacca tttggagtat
     8041 ttggtaaaaa gatggtttga tttcctttag aggcaaaggt attcaaggta tccaaatact
     8101 ggttggtcaa gaggatagac atgatttgtt cttctgtcat gccaacattg gcttccttga
     8161 gttcggtgat agactctgcc aatccatcca caatcgcctt acgttgttgg gcaatcccca
     8221 caccatgaag gcggtctttt tctgcttctg cttcagctgc agtgacaatt ttaatcttgt
     8281 cagcttccgc caattcttgt gctgcgaccc gcttacgttg cgccgcattg atttcattca
     8341 tggattgctt aacttctgca tctggttcga ccttggtaat caaggttttc acgataatgt
     8401 agccgtaagt ggtcatttct tctgctactt ggtgttgaac ctcaagggca atctcatctt
     8461 ttttctcaaa caattcatcc aaggttaatt ttggaacaga agagcgaaga gcatcttcga
     8521 tataagattt aatctgagat tctggacgta tgagtttata gtaagcatct gtcacgctct
     8581 gctcgttgac acggtactga gtcgctacat tcatcataac gaacacattg tccttggtct
     8641 tagtctcaac cacaatatca ctttgcaaca agcgcaactg aatccgtgct gcaatcgagt
     8701 caatcccaaa aggcaagcga atatgaatac cgctattagc aaccttttgg tatttcccaa
     8761 agcgttcaat aatcgccacc gactgctgac gaaccacata aactgtactc agtgtgacta
     8821 tcaccaatag gagcacacaa acaatcagaa aaatcatgaa aaatattgcc ataatggaac
     8881 ctccacaagt atttttctag tattatagca catttaaaga aggctgtgcc gtttttactg
     8941 cgatttttcc tgaaatgtca ataattagag gtgaattgtc ctattgtcgt ccaatctctt
     9001 gctaaaataa ctctttataa aaggcaatcg tttcttctaa ggttggcata aatggatttc
     9061 ctggtgcgca ggcatcaatc aaggcattct tagaaaggta ttcaaagtcg aaatcttttt
     9121 cttcaatacc aagttcagtc agtttcttag gaatacctac tgtctcagaa agcttctcaa
     9181 tctcagcaat cgcataatcg gcacattctt gatctgattt accttctaca tgaagtccca
     9241 aggctttggc aacattgcgg aaagcttctg gtacacgttt agcattttca cgttctataa
     9301 ctggtagcaa catggcacag cacacgccat gtggcaaatt atatactgca ccgagttggt
     9361 gagccattga gtgaacataa ccaagaccag cattattaaa gctcatatca ccaaggaaga
     9421 tggcattgac cataccttca cgcgcttcaa tatcatgtcc attttctaca gcacgaggaa
     9481 gatattcctt gataagctca atagcaccaa tagacagttt cttggttaca tcataagcac
     9541 caggtgttac caaagcttca acagcgtgag tcagagcatc cataccagta gcagcagtca
     9601 ggcctttagg tttggaaagc atgagttccg ggtcattaac agagataaga gcaaggctat
     9661 tcttgtcaac cattaccatc ttaaccttgc gttcttcatc agtaatcaca tagttaatag
     9721 tgatttctgc agaagttcct gccgttgtat tgatagccac aactggcaag ccttttttag
     9781 cagacttatg aagcccttca taatcctgtg gttttccacc atttgtagcc atgatagaaa
     9841 tacaactagc tgcatcctgt ggagaacccc cgccaagact gatgataaag tcacagccat
     9901 gttcttgcaa agcagctacc ccatctgtga catttttaca agtagggttt ggctctacat
     9961 cgctaaagat gacatattcg attccttctg tatccagtgg ttttaaagtc ttaggtaaaa
    10021 tatcactgcc ttcgatgtac ttatctgtaa ccaaaagtgc ctttttatag ccaagttcct
    10081 tgatataagg acccacttca tttacaacac cttttccaat aaggttgact gccggaacat
    10141 aaaatgtagc catataattt tcctcctgcg cctccgcgct atctatttaa catcattata
    10201 ttactttttg tattcgtttt taattatttg tgaattgttt aacaaatctt tttaaaagga
    10261 taggagggac gtaagttcac ctcctatata aacaagtttt atttatgtag tggtcccaac
    10321 aactgacatg cacaatagtc tgctgattct agatcttctg ttccaaataa ggaccaattt
    10381 ttaggtctaa agatatcttc ctcaggtaca ttatgcatat tgacaggaat tctcaacata
    10441 gaagccaagg taatcaagtc tgctccaatg tgtccatatg ttatggctcc gtgattagct
    10501 ccccaattat tcatgacgtc atagacagac ttgaaagcac cttttcctgt caaacgtgga
    10561 gcaaaccaag tagttggcca tcctggatct gtacgattat ctaaagtatg gtgaacatct
    10621 tcaggaagtt caagtgtata accttctgca atttgtagca ctggaccaac cccttttaga
    10681 agattgagac gtaccattgt tactggcata tcccccttcg tcaagaaacg agttgagaat
    10741 cctcctccac ggaagtattc gcggtttgct ggtgggaagt ctgtattttc aagcatagcc
    10801 tgcacttcac tttcttccaa ctcccagaat ggtttcataa caggtttgcc atctcgagta
    10861 gcttgacctg taccatccaa tgtacaagaa ccagagttga ttagatgtaa gaagccagct
    10921 gcagcacgac cctctaaagt atgtcccgtt acacgtttaa cagcctctgg gctccaataa
    10981 gtacgcacat cagcaaagat ttgtggagta tttgttaata gataattaaa gagcatagac
    11041 acaccattta gtgaatcatt ctctgtcgca aatacaaatg gttttcgaat accattccag
    11101 tcaaactgag tattgaggaa agtttccata aaatctccat ttggaaaatg gtctgtccac
    11161 tgacgttgac cttggaaacc agctactaaa gcatggtgac caactgcttc ttcctcaaaa
    11221 ccaagttcag caagtcttgg gttaccaacc attaagtcac gtccaatcat gaacatctta
    11281 ataacaaatt cccattgtct atctttttct tcacggctta aaacaaggtc ttcacggtta
    11341 tggtcgaatc cttcttttac gttttctttc acccatttga gcgcacgttc gaactcttca
    11401 gggtcgtaaa tactacgatc catacggcgc gtgaactccg tcatatctac cgattcattt
    11461 cgcattccta ggtattcttg gaagaaatcc ggatttacaa tagaaccacc aatccccatc
    11521 gaaacactac ccattgatag gtaagcagtg tctctcatca agccagttgc aagagctgca
    11581 cgcgcatagc gtaaaagttt ttctttgaca tcttctggaa tatctgtgtc actaacttcc
    11641 tgaacatctc ttccataaat cccaaaggct ggaatccctt tttgagcatg tgaagctagt
    11701 acagctgcaa gatagacagc tcctgggcgt tctgtcccat taaatcccca aatagcatga
    11761 ggaatatctg gagacatatc catagtttca ctaccataac accagcatgg tgtaactgta
    11821 attgttgcgc aaacatttga ttttttaaac aactcatggg aagctgcagc ctctggtaca
    11881 cggccaatag tagatggaga aatcacgcat tccacaggtt ccccatctgg atatttcaat
    11941 gtgcttgaaa tcaaatctgc cacactttta gccatgttca ttgtttgcac ttcaagtgat
    12001 tcgcgtacac cttgacgacg accatcaata gtcggacgaa tcccaatacg tggatgttga
    12061 atcatactat tttcctctta tttttctgtt tctttcatac taccatctcc attatattta
    12121 cggtagcctg ggtgataatt tttaaaatta cttataagta taaacctcta cctctgctaa
    12181 acttaaagga attcctgcgc ttctcaaaga aattctaacc ttttttccct tcagtccttt
    12241 taagtctatg gatagattat tgctttctaa actgtcaata tgttgtgtaa aaacttcata
//
LOCUS       DAVHEW010000056        12280 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVHEW010000056 DAVHEW010000000
VERSION     DAVHEW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.056"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            211..2871
                     /gene="rrgA"
                     /locus_tag="VVL53_001415"
     CDS             211..2871
                     /gene="rrgA"
                     /locus_tag="VVL53_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU4183575.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDSENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRGAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3095..5092
                     /gene="rrgB"
                     /locus_tag="VVL53_001416"
     CDS             3095..5092
                     /gene="rrgB"
                     /locus_tag="VVL53_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HEU4183576.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAAIMGIAVYAYVKNNKDEDQLA"
     gene            5139..6320
                     /gene="rrgC"
                     /locus_tag="VVL53_001417"
     CDS             5139..6320
                     /gene="rrgC"
                     /locus_tag="VVL53_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU4183577.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDDRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKTDTMTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDVSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIFVTNLPLGNY
                     RFKEVEPLAGYAVTTLDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEESGHYTPVLQNGKEVVVTSGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKPN
                     N"
     gene            6658..7560
                     /gene="srtC-1"
                     /locus_tag="VVL53_001418"
     CDS             6658..7560
                     /gene="srtC-1"
                     /locus_tag="VVL53_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU4183578.1"
                     /translation="MVKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            7547..8440
                     /gene="srtC-2"
                     /locus_tag="VVL53_001419"
     CDS             7547..8440
                     /gene="srtC-2"
                     /locus_tag="VVL53_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU4183579.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            8427..9278
                     /gene="srtC-3"
                     /locus_tag="VVL53_001420"
     CDS             8427..9278
                     /gene="srtC-3"
                     /locus_tag="VVL53_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEU4183580.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(9351..10627)
                     /locus_tag="VVL53_001421"
                     /pseudo
     CDS             complement(9351..10627)
                     /locus_tag="VVL53_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10742..10882)
                     /locus_tag="VVL53_001422"
     CDS             complement(10742..10882)
                     /locus_tag="VVL53_001422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183581.1"
                     /translation="MKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVDAFM
                     AL"
     gene            complement(10884..11168)
                     /locus_tag="VVL53_001423"
     CDS             complement(10884..11168)
                     /locus_tag="VVL53_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183582.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
BASE COUNT         3894 a         1996 c         2761 g         3629 t
ORIGIN      
        1 tatacaagtg tgtcattgcc aggttgagaa gatagctata acgcactttt atacgctttt
       61 gctacgtttg ttagtgaacg gattaactca gtgagataaa ttttatcaga acataagtaa
      121 tccgtttctt cgtgtataca gattgaaagt acctatgaat catagaagga ttaacttgtt
      181 ctatgaataa tgcttaacag ggagacacac atgaaaaaag taagaaagat atttcagaag
      241 gcagttgcag gactgtgctg tatatctcag ttgacagctt tttcttcgat agttgcttta
      301 gcagaaacgc ctgaaaccag tccagcgata ggaaaagtag tgattaagga gacaggcgaa
      361 ggaggagcgc ttctaggaga tgccgtcttt gagttgaaaa acaatacgga tggcacaact
      421 gtttcgcaaa ggacagaggc gcaaacagga gaagcgatat tttcaaacat aaaacctggg
      481 acatacacct tgacagaagc ccaacctcca gttggttata aaccctctac taaacaatgg
      541 actgttgaag ttgagaagaa tggtcggacg actgtccaag gtgaacaggt agaaaatcga
      601 gaagaggctc tatctgacca gtatccacaa acagggactt atccagatgt tcaaacacct
      661 tatcagatta ttaaggtaga tggttcggaa aaaaacggac agcacaaggc gttgaatccg
      721 aatccatatg aacgtgtgat tccagaaggt acactttcaa agagaattta tcaagtgaat
      781 aatttggatg ataaccaata tggaatcgaa ttgacggtta gtgggaaaac agtgtatgaa
      841 caaaaagata agtctgtgcc gctggatgtc gttatcttgc tcgataactc aaatagtatg
      901 agtaacattc gaaacaagaa tgctcgacgt gcggaaagag ctggtgaggc gacacgttct
      961 cttattgata aaattacatc tgattcagaa aatagggtag cgcttgtgac ttatgcttcc
     1021 actatctttg atgggaccga gtttacagta gaaaaagggg tagcagataa aaacggaaag
     1081 cgattgaatg attctctttt ttggaattat gatcagacga gttttacaac caataccaaa
     1141 gattatagtt atttaaagct gactaatgat aagaatgaca ttgtagaatt aaaaaataag
     1201 gtacctaccg aggcagaaga ccatgatgga aatagattga tgtaccaatt cggtgccact
     1261 tttactcaga aagctttgat gaaggcagat gagattttga cacaacaagc gagacaaaat
     1321 agtcaaaaag tcattttcca tattacggat ggtgtcccaa ctatgtcgta tccgattaat
     1381 tttaatcatg ctacgtttgc tccatcatat caaaatcaac taaatgcatt ttttagtaaa
     1441 tctcctaata aagatggaat actattaagt gattttatta cgcaagcaac tagtggagaa
     1501 catacaattg tacgcggaga tgggcaaagt taccagatgt ttacagataa gacagtttat
     1561 gaaaaaggtg ctcctgcagc tttcccagtt aaacctgaaa aatattctga aatgaaggcg
     1621 gctggttatg cagttatagg cgatccaatt aatggtggat atatttggct taattggaga
     1681 gagagtattc tggcttatcc gtttaattct aatactgcta aaattaccaa tcatggtgac
     1741 cctacaagat ggtactataa cgggaatatt gctcctgatg ggtatgatgt ctttacggta
     1801 ggtattggta ttaacggaga tcctggtacg gatgaagcaa cggctactag ttttatgcaa
     1861 agtatttcta gtaaacctga aaactatacc aatgttactg acacgacaaa aatattggaa
     1921 cagttgaatc gttatttcca caccatcgta actgaaaaga aatcaattga gaatggtacg
     1981 attacagatc cgatgggtga gttaattgat ttgcaattgg gcacagatgg aagatttgat
     2041 ccagcagatt acactttaac tgcaaacgat ggtagtcgct tggagaatgg acaagctgta
     2101 ggtggtccac aaaatgatgg tggtttgtta aaaaatgcaa aagtgctcta tgatacgact
     2161 gagaaaagga ttcgtgtaac aggtctgtac cttggaacgg atgaaaaagt tacgttgacc
     2221 tacaatgttc gtttgaatga tgagtttgta agcaataaat tttatgatac caatggtcga
     2281 acaaccttac atcctaagga agtagaacag aacacagtgc gcgacttccc gattcctaag
     2341 attcgtgatg tgcggaagta tccagaaatc acaatttcaa aagagaaaaa acttggtgac
     2401 attgagttta ttaaggtcaa taaaaatgat aaaaaaccac tgagaggtgc ggtctttagt
     2461 cttcaaaaac aacatccgga ttatccagat atttatggag ctattgatca aaatggcact
     2521 tatcaaaatg tgagaacagg tgaagatggt aagttgacct ttaaaaatct gtcagatggg
     2581 aaatatcgat tatttgaaaa ttctgaacca gctggttata aacccgttca aaataagcct
     2641 atcgttgcct tccaaatagt aaatggagaa gtcagagatg tgacttcaat cgttccacaa
     2701 gatataccag cgggttacga gtttacgaat gataagcact atattaccaa tgaacctatt
     2761 cctccaaaga gagaatatcc tcgaactggt ggtatcggaa tgttgccatt ctatctgata
     2821 ggttgcatga tgatgggagg agttctatta tacacacgga aacatccgta aagtgtagaa
     2881 atgataatat ctatgttctg aacgatactt ttaagaagta gcactcaaga agagatttaa
     2941 gtttacttgg tgaaacctgt tttattcgta agtaaactat cattgaaagg ggagatgttt
     3001 tcgaaaactt gcacagaaaa aggattatta ttgtcatgtg taattcatta cattgctcac
     3061 agttgatttt aagagatatg aataaggaga aatcatgaaa tcaatcaaca aatttttaac
     3121 aatgcttgct gccttattac tgacagcgag tagcctgttt tcagctgcaa cagtttttgc
     3181 ggctgggacg acaacaacat ctgttaccgt tcataaacta ttggcaacag atggggatat
     3241 ggataaaatt gcaaatgagt tagaaacagg taactatgct ggtaataaag tgggtgttct
     3301 acctgcaaat gcaaaagaaa ttgccggtgt tatgttcgtt tggacaaata ctaataatga
     3361 aattattgat gaaaatggcc aaactctagg agtgaatatt gatccacaaa catttaaact
     3421 ctcaggggca atgccggcaa ctgcaatgaa aaaattaaca gaagctgaag gagctaaatt
     3481 taacacggca aatttaccag ctgctaagta taaaatttat gaaattcaca gtttatcaac
     3541 ttatgtcggt gaagatggag caaccttaac aggttctaaa gcagttccaa ttgaaattga
     3601 attaccattg aacgatgttg tggatgcgca tgtgtatcca aaaaatacag aagcaaagcc
     3661 aaaaattgat aaagatttca aaggtaaagc aaatccagat acaccacgtg tagataaaga
     3721 tacacctgtg aaccaccaag ttggagatgt tgtagagtac gaaattgtta caaaaattcc
     3781 agcacttgct aattatgcaa cagcaaactg gagcgataga atgactgaag gtttggcatt
     3841 caacaaaggt acagtgaaag taactgttga tgatgttgca cttgaagcag gtgattatgc
     3901 tctaacagaa gtagcaactg gttttgattt gaaattaaca gatgctggtt tagctaaagt
     3961 gaatgaccaa aacgctgaaa aaactgtgaa aatcacttat tcggcaacat tgaatgacaa
     4021 agcaattgta gaagtaccag aatctaatga tgtaacattt aactatggta ataatccaga
     4081 tcacgggaat actccaaagc cgaataagcc aaatgaaaac ggcgatttga cattgaccaa
     4141 gacatgggtt gatgctacag gtgcaccaat tccggctgga gctgaagcaa cgttcgattt
     4201 ggttaatgct cagactggta aagttgtaca aactgtaact ttgacaacag acaaaaatac
     4261 agttactgtt aacggattgg ataaaaatac agaatataaa ttcgttgaac gtagtataaa
     4321 agggtattca gcagattatc aagaaatcac tacagctgga gaaattgctg tcaagaactg
     4381 gaaagacgaa aatccaaaac cacttgatcc aacagagcca aaagttgtta catatggtaa
     4441 aaagtttgtc aaagttaatg ataaagataa tcgtttagct ggggcagaat ttgtaattgc
     4501 aaatgctgat aatgctggtc aatatttagc acgtaaagca gataaagtga gtcaagaaga
     4561 gaagcagttg gttgttacaa caaaggatgc tttagataga gcagttgctg cttataacgc
     4621 tcttactgca caacaacaaa ctcagcaaga aaaagagaaa gttgacaaag ctcaagctgc
     4681 ttataatgct gctgtgattg ctgccaacaa tgcatttgaa tgggtggcag ataaggacaa
     4741 tgaaaatgtt gtgaaattag tttctgatgc acaaggtcgc tttgaaatta caggccttct
     4801 tgcaggtaca tattacttag aagaaacaaa acagcctgct ggttatgcat tactaactag
     4861 ccgtcagaaa tttgaagtca ctgcaacttc ttattcagcg actggacaag gcattgagta
     4921 tactgctggt tcaggtaaag atgacgctac aaaagtagtc aacaaaaaaa tcactatccc
     4981 acaaacgggt ggtattggta caattatctt tgctgtagcg ggggctgcga ttatgggtat
     5041 tgcagtgtac gcatatgtta aaaacaacaa agatgaggat caacttgctt aagtaagaga
     5101 gaaaggagcc attgatgaca atgcagaaaa tgcagaaaat gattagtcgt atcttctttg
     5161 ttatggctct gtgtttttct cttgtatggg gtgcacatgc agtccaagcg caagaagatc
     5221 acacgttggt cttgcaattg gagaactatc aggaggtggt tagtcaattg ccatctcgtg
     5281 atggtcatcg gttgcaagta tggaagttgg atgattcgta ttcctatgat gatcgggtgc
     5341 aaattgtaag agacttgcat tcgtgggatg agaataaact ttcttctttc aaaaagactt
     5401 cgtttgagat gaccttcctt gagaatcaga ttgaagtatc tcatattcca aatggtcttt
     5461 actatgttcg ctctattatc cagacggatg cggtttctta tccagctgaa tttctttttg
     5521 aaatgacaga tcaaacggta gagcctttgg tcattgtagc gaaaaaaaca gatacaatga
     5581 caacaaaggt gaagctgata aaggtggatc aagaccacaa tcgcttggag ggtgtcggct
     5641 ttaaattggt atcagtagca agagatgttt ctgaaaaaga ggttcccttg attggagaat
     5701 accgttacag ttcttctggt caagtaggga gaactctcta tactgataaa aatggagaga
     5761 tttttgtgac aaatcttcct cttgggaact atcgtttcaa ggaggtggag ccactggcag
     5821 gctatgctgt tacgacgctg gatacggatg tccagctggt agatcatcag ctggtgacga
     5881 ttacggttgt caatcagaaa ttaccacgtg gcaatgttga ctttatgaag gtggatggtc
     5941 ggaccaatac ctctcttcaa ggggcaatgt tcaaagtcat gaaagaagaa agcggacact
     6001 atactcctgt tcttcaaaat ggtaaggaag tagttgtaac atcagggaaa gatggtcgtt
     6061 tccgagtgga aggtctagag tatgggacat actatttatg ggagctccaa gctccaactg
     6121 gttatgttca attaacatcg cctgtttcct ttacaatcgg gaaagatact cgtaaggaac
     6181 tggtaacagt ggttaaaaat aacaagcgac cacggattga tgtgccagat acaggggaag
     6241 aaaccttgta tatcttgatg cttgttgcca ttttgttgtt tggtagtggt tattatctta
     6301 cgaaaaaacc aaataactga tattcaatgt acatcattat gaaaaagata gcaggctgaa
     6361 gggaagacca gagtactctg aggtgatgtt aatcaggaat catggtgatg tggcatgaat
     6421 cacaataacg gatatgaggc tgggcagatt gtgccagcct cattgtgggt tattgtttgt
     6481 aaaacgatag gactggtctg gtaatcattt taggaatgga caggactggg attctgattt
     6541 aaaatggatg gtgaatcaga aagaaatgag attttctcgt ttctcttagc agataggatt
     6601 gtctgttagg aaaagcgata aaatgatgag tttgaagata aagggatgct gataaaaatg
     6661 gtaaaaacaa aaaagcaaaa acgaaataat ctcctattag gagtggtatt tttcattgga
     6721 atggcggtaa tggcgtatcc gctggtgtct cgcttgtatt atcgagtgga atcaaatcaa
     6781 caaattgctg actttgataa ggaaaaagca acgttggatg aggctgacat tgatgaacga
     6841 atgaaattgg cacaagcctt caatgactct ttgaataatg tagtgagtgg cgatccttgg
     6901 tcggaagaaa tgaagaaaaa agggcgagca gagtatgcac gtatgttaga aatccatgag
     6961 cggatggggc atgtggaaat ccccgttatt gacgtggatt tgccggttta tgctggtact
     7021 gctgaagagg tattgcagca aggggctggg catctagagg gaacttctct gccgatcgga
     7081 ggcaattcga cccatgcggt gattacggca catacaggtt tgccaacagc taagatgttt
     7141 acggatttga ccaaacttaa agttggggat aagttttatg tgcacaatat caaggaagtg
     7201 atggcctatc aagtggatca agtaaaggtg attgagccga cgaactttga tgatttattg
     7261 attgtaccag gtcatgatta tgtgaccttg ctgacttgta cgccatacat gatcaatacc
     7321 catcgtctat tggttcgggg gcatcggata ccgtacgtag cagaggttga ggaagaattt
     7381 attgcagcaa acaaactcag tcatctctat cgctacctgt tttatgtggc agttggtttg
     7441 attgtgattc ttttatggat tattcgacgc ttgcgcaaga agaaaaaaca accggaaaag
     7501 gctttgaagg cgctgaaagc agcaaggaag gaagtgaagg tggaggatgg acaacagtag
     7561 acgttcacga aaaaaaggca caaaaaagaa gaaacatccg ctgatccttc ttctgatttt
     7621 cttagtagga ttcgccgttg cgatatatcc attggtgtct cgttattatt atcgtattga
     7681 gtcaaacgag gttattaaag agtttgatga gacggtttcc cagatggata aggcagaact
     7741 tgaggagcgt tggcgcttgg ctcaagcctt caatgcgacc ttgaaaccat ctgaaattct
     7801 tgatcctttt acagagcaag agaaaaagaa aggcgtctca gaatatgcca atatgctaaa
     7861 ggtccatgag cggattggct atgtggaaat tcctgcgatt gatcaggaaa ttccgatgta
     7921 tgtcggaacg agtgaggaca ttcttcagaa aggggcaggg ctgttagaag gggcttcgct
     7981 gcctgttgga ggtgaaaata cccatacagt gatcactgct cacagaggat tgccaacggc
     8041 agaattgttc agtcaattgg ataagatgaa aaaaggggat atcttttatc ttcacgtttt
     8101 agatcaggtg ttggcctacc aagtggatca gatagtgacg gtggagccga atgactttga
     8161 gcctgtcttg attcaacatg gggaagatta tgcgaccttg ttgacttgta caccgtatat
     8221 gattaacagt catcgtctgt tggtacgtgg gaagcggatt ccgtatacgg caccaattgc
     8281 agagcggaat cgagcggtga gagagcgtgg gcaattctgg ttgtggttat tactaggagc
     8341 gatggcggtc atccttctct tgctgtatcg cgtgtatcgt aatcgacgga ttgtcaaagg
     8401 actagaaaag caattggagg ggcgtcatgt caaggactaa actacgagcc ttattgggat
     8461 acttgttgat gttggtagcc tgtttgattc ctatttattg ttttggacag atggtgttgc
     8521 agtctcttgg acaggtgaaa ggtcatgcta catttgtgaa atccatgaca actgaaatgt
     8581 accaagaaca acagaaccat tctctcgcct acaatcaacg cttggcttcg caaaatcgca
     8641 ttgtagatcc ttttttggcg gagggatatg aggtcaatta ccaagtgtct gacgaccctg
     8701 atgcagtcta tggttacttg tctattccaa gtttggaaat catggagccg gtttatttgg
     8761 gagcagatta tcatcattta gggatgggct tggctcatgt ggatggtaca ccgctgcctc
     8821 tggatggtac agggattcgc tcagtgattg ctgggcaccg tgcagagcca agccatgtct
     8881 ttttccgcca tttggatcag ctaaaagttg gagatgctct ttattatgat aatggccagg
     8941 aaattgtaga atatcagatg atggacacag agattatttt accgtcggaa tgggaaaaat
     9001 tagaatcggt tagctctaaa aatatcatga ccttgataac ctgcgatccg attcctacct
     9061 ttaataaacg cttattagtg aattttgaac gagtcgctgt ttatcaaaaa tcagatccac
     9121 aaacagctgc agttgcgagg gttgctttta cgaaagaagg acaatctgta tcgcgtgttg
     9181 caacctctca atggttgtac cgtgggctag tggtactggc atttctggga atcctgtttg
     9241 ttttgtggaa gctagcacgt ttactacgag ggaaataaaa agaaatgaaa ggaaagctaa
     9301 ggctgttcct ttttccggct ctttgtcaac tgtagtgggt tgaaaaaaag ctaagctcga
     9361 gaaaggacaa attttgtcct ttcttttttg atattcagag cgataaaaat ccgttttttg
     9421 aagttttcaa agtttcgaaa accaaaggca ttgcgcttga taagtttgat gagattattg
     9481 gtcgcttcca gtttggcatt agaatagtgt agttgaaggg cgttgataac cttttcttta
     9541 tctttgagga aggttttaaa gacagtctga aaaataggat gaacctgctt aagattgtcc
     9601 tcaataagtc cgaaaaattt ctccggttcc ttattctgaa agtgaaacag caagagttga
     9661 tagagctgat agtgatgttt caagtcttgt gaatagctca aaagcttgtc taaaatctct
     9721 ttattggtta aatgcatacg aaaagtaggg cgataaaaat gtttatcgct gagtttacgg
     9781 ctatcctgtt gaatgagttt ccagtagcgc ttgatagcct tgtattcatg ggattttcga
     9841 tgaaactgat tcatgatttg gatacgcaca cgactcatag cacggctaag atgttgtaca
     9901 atgtgaaagc gatcaagaac gattttagca cacgggaaaa gttgtctagc taagtcatag
     9961 taaggactaa acatatccat agtaatgatt ttgacgcgac atcggacggc tctatcatat
    10021 ttaagaaagt gatctcggat gacagcttgt gttctacctt caagaacagt gatgatatcg
    10081 agcttttcaa aatcttgcgc aataaagctc atctccatct cccgattgaa acagtcactc
    10141 cccggactgt ttcaacgtcc caggacataa tctcaggaag acgcgaaaaa tcatgctcaa
    10201 agtgagaatc attgagcttg cgaatgacag ttgaagttga aatggccagc tgatgagcaa
    10261 tatcggtcat agaaatcttc tcaatcaact tttgcgcaat tttttggttg ataatacgag
    10321 gaatttgatg attcttcttg acgatagaag tttcagcgac catcattttt gaacagtgat
    10381 agcacttgaa acggcgtttt ctaaggagaa ttctagaagg cataccagtt gtttcgaggt
    10441 aagggatctt agacggtttt tgaaagtcat atttcttcaa ttggtttccg cactcagggc
    10501 aagatggggc gtcgtagtcc agtttggcga tgatttcctt gtgtgtatcc ttattgatga
    10561 tgtctaaaat ctggatatta gggtctttaa tgtctagtaa ttttgtgata aaatgtaatt
    10621 gttccatatg attctttcta atgagttgtt ttgtcgcttt tcattatagg tcatatggga
    10681 ctttttttct acacaaaaat aggctccata atatccatag gggatttacc cactacaaat
    10741 attatagagc cataaaagca tcaacatctt gtgtggcacg taaaccatga tattctaaga
    10801 cataaccacc gacacagata attgttaaag tcagattttc ctgaattaat tccttattta
    10861 gggcttggaa gaccggtttc attttatcca tgtttgtatt tctccatcaa tttttgaaca
    10921 ttggttaact tagcaggttt atttaatgaa atcacatatt tatgagttgg agtatgctct
    10981 tgaaaggcat acttcaagct aattttttga aaacgggtta gttctttgga gtctagcaac
    11041 ttttctacat cttggtttgt taaataagaa tagtcttcaa aaataacttt tcgcaaatag
    11101 cgaaaccaat ggcttgcgtt gaattgtgat tgtttggatg cctgtatcat ataatcgtac
    11161 caatccattg tcttcctcct ataaaattta gtttatatta ttgtagttta tattcaaaaa
    11221 tgtagttttt cttaattagc tcgtatctat aggatgatac tgaaaatctt cttgttgctt
    11281 taaatcacaa tcgaaaaatt ctgtttttca tcttagaaaa gaatgtttag aaatgtaaaa
    11341 tgaacctttt ttcttatccg aaaaccttac taaagtatat aatttaggaa gtcaatcctg
    11401 tatgataatg tttgtactat cgtcgagttt ttgcttgatt gcctaacgag ataaatttta
    11461 gaagaatttt cggtgtgatt tgacaagctt ctgactggaa ggatagttac actagtattg
    11521 tttattaaaa tgagcagata gagaaaattt ggaatctatt tgcttattat caatcgtttt
    11581 tttagcttat ttgtcttaga ttggtataga actgagctat ggattaatac tcttgtatct
    11641 tatcctgttc ctaaatatgt tggtagaaaa atgaagcagt tgttacgagt gtctgtattg
    11701 gaaaaaattt tgtctataga attctttaaa tgatatgaag gctaggaatg ccaaagcact
    11761 gacatatttc gtttgaatct catccgctct ctttacttgt atttgtagaa atgctatcag
    11821 taatatattc gcgaacacta tgatttacaa cagtgtggtc gtacatcttt gtaactttat
    11881 tatacaacaa ccatttacag gtgtaaagtt gagcagtgtc tttagtaaat ttagggattt
    11941 gttttgtcat agttttgata cctgtattcg ttgtttcatg agcatttgac gtttcttttc
    12001 atgtaggcat ttcaacaatt agaatgatat ttttccgagg atgaacttat atattgtgac
    12061 tttttagagt tatgatgcct cttgatttga ctatcgctat caatttcatc cacttttaca
    12121 gtttcatggt tttatgaacc gaaacatcta ggtacaggtt ttagcatcaa ttgattgttt
    12181 ttgagccata tgaagttgaa acgtggagat gcttttcttg tgataacgcc ctattcaaag
    12241 aggaataacc gtttttggag agagttcagt actatgtggc
//
LOCUS       DAVHEW010000057        12235 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVHEW010000057 DAVHEW010000000
VERSION     DAVHEW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.057"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            489..2168
                     /locus_tag="VVL53_001424"
     CDS             489..2168
                     /locus_tag="VVL53_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU4183583.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(2208..4292)
                     /locus_tag="VVL53_001425"
     CDS             complement(2208..4292)
                     /locus_tag="VVL53_001425"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU4183584.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEGGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            4463..5743
                     /locus_tag="VVL53_001426"
     CDS             4463..5743
                     /locus_tag="VVL53_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU4183585.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            5834..8479
                     /locus_tag="VVL53_001427"
     CDS             5834..8479
                     /locus_tag="VVL53_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU4183586.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMCSENVRVPESQHLFLDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            8562..9431
                     /locus_tag="VVL53_001428"
     CDS             8562..9431
                     /locus_tag="VVL53_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4183587.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPGVISLGGSISQNPDFIQGVKKAAEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            9425..11305
                     /locus_tag="VVL53_001429"
     CDS             9425..11305
                     /locus_tag="VVL53_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEU4183588.1"
                     /translation="MVRFTGLSLKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEILALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDVTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            11457..11666
                     /locus_tag="VVL53_001430"
     CDS             11457..11666
                     /locus_tag="VVL53_001430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183589.1"
                     /translation="MKQKNYLVSNATVRQTYDKIAESECFLRAIGGIMLHLKLVKQEI
                     EAEKPASVEAWIISVKFKKGCYRHI"
     gene            11667..12029
                     /locus_tag="VVL53_001431"
     CDS             11667..12029
                     /locus_tag="VVL53_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183590.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFRS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(12081..>12235)
                     /locus_tag="VVL53_001432"
     CDS             complement(12081..>12235)
                     /locus_tag="VVL53_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4183591.1"
                     /translation="ALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGII
                     NHELGF"
BASE COUNT         3449 a         2489 c         2963 g         3334 t
ORIGIN      
        1 catctggtta tcacttggat ctgtataaag agaaatgttt gtgtgaaagg ataaaaagat
       61 gcaacattct tttgaagata gattttggag aagcatttct tgttcaattg tacaaattat
      121 agaatctttt ctgagtattt aggaagagat aattatctta ttgaccctct ttttattttt
      181 tgttatagtc gacgagatga tagtttagaa taatcactta ttcacaatct gtggaaaagt
      241 ctggatagtg aatggtcttc acacaatgac ctgaaagaag cctgagaata attatggaga
      301 gtagcattct gagaggtgtt agcagaacca tatgacagag ctgtttgaag agggaacatt
      361 gaggagaaaa atcctgagcc taccagttgg agttggaaag agctggctgt tagatcatgg
      421 tttattatcc acaacctgtg gataatcttg tgaacaagag aagttgcaaa agaaggagat
      481 atataacgat gaagaaaatc aaaccgcatg gaccgttacc aagtcagact cagctagctt
      541 atctgggaga tgaactagca gcttttatcc acttcggtcc taataccttt tatgaccaag
      601 aatgggggac tggacaggag gatcctgagc gctttaaccc gagtcagttg gatgcgcgtg
      661 agtgggttcg tgtgctcaag gaaacgggct tcaaaaagtt gattttggtg gtcaagcacc
      721 acgatggctt tgtcctttat ccgacagctc acacagatta ttcggttaag gtcagtcctt
      781 ggaggagagg aaagggcgac ttgctccttg aagtatccca agctgccaca gagtttgata
      841 tggatatggg ggtctacctg tcaccgtggg atgcccatag tcccctctat catgtggacc
      901 gagaagcgga ctacaatgcc tattatctgg ctcagttgaa ggaaatctta tcaaatccta
      961 actatgggaa tgctggtaag ttcgctgagg tttggatgga tggtgccaga ggagagggcg
     1021 cgcaaaaggt taattatgaa tttgaaaaat ggtttgaaac cattcgtgac ctgcagggcg
     1081 attgcttgat tttttcaaca gaaggcacca gtatccgctg gattggcaat gaacgagggt
     1141 atgcaggtga tccactgtgg caaaaggtga atcttgataa actaggaaca gaagcagagc
     1201 tgaactatct tcagcacggg gatccctcgg gcacgatttt ttcaatcgga gaggcagatg
     1261 tttccatccg tccaggctgg ttttaccatg aggatcagga tcctaagtct ctcgaggagt
     1321 tggtcgaaat ctactttcac tcagtagggc gaggaactcc actcttgctt aatattccgc
     1381 cgaatcaagc tgggctcttt gatgcaaagg atattgaacg actttatgaa tttgcgacct
     1441 atcgcaatga gctctataaa gaagatttgg ctctgggagc tgaggtatct ggtccagctc
     1501 tttccgcaga ctttgattgt cgccatttga cagacggcct tgagaccagc tcttgggcaa
     1561 gcgatgcaga cttgcccatc cagttagaac tcgatttagg ttctcctaaa acttttgatg
     1621 taattgagtt aagagaagat ttgaagctag ggcaacgaat cgctgctttt catgtgcaag
     1681 tagaggtgga tggtgtctgg caggagtttg gcaggggctt cacagttggt cataagcgcc
     1741 tcttgcgagg tccgctagta gaggcacaaa aggtgcgcgt gatgattacc gaggcacaat
     1801 ctatccctgt attgaccaag atttcccttt ataaaactcc tgggttatca aaaaaagaag
     1861 ttgttcagga actagcattt gcagaaaaaa gcctagctgt ggcaaaggga gagactcttc
     1921 attttaggat tgaacgtagt gaaagtagct cctctttaga ggctaagatt tcgattcaac
     1981 cggggacagg tgtccatggt gtcgcctatc aggacgagat ttatgtcctt gcgtttcaag
     2041 ctggtgagac tgaaaaaagg ctgacgctac caaccttgta tttcgcagga gataaaacct
     2101 tggatttcta tctgaaccta acggtggatg gtcagcttgt ggatcaactt caagtccaag
     2161 tttcataaaa gaagaacctt tgcgcgatgc aaaggttctt ttggttatta gtgatttggt
     2221 agccagctga gggtgaaagt tagttgttca gcttttagga ggtcttggtg ttgaatagtt
     2281 gatacgagtg ttttgtccag tcggcattct ttgacaaagt taaaatggtt gtggttttgt
     2341 ttagtatgaa tatctagcca tttattttct ttagctaggt agactcgtag atggtcaaag
     2401 agaggaattc cgagatcata gcttggtttt cctggacagg ttggataaaa tccgagagct
     2461 gaccagatgt accaagcaga gagactacca ttgtcttcat ctccaggata ggcttcccaa
     2521 cttgggtgaa aagctttctg acggagcgtc ttgataagaa gggcagtgta gtcagggtaa
     2581 tcgctgtagc ggaagagata aggaatgtgg aaactaggct ggttggaaat ggctatttgt
     2641 ccaaaaggag cagtagccat ctcgctcatt tcgtgaattt cgtaaccata gcctgttgtt
     2701 tcaaagaggg gagcatcttg acaggctttc aaaagatagt tgctaaaggt ttcttttcca
     2761 cccatcagtt ggattaagcc agggatgtcg tggagaacgc ctaaagtagc ttgaatggca
     2821 gagcattcag cgtagtctcg cccccaacta taaggagaga agtcagggtg aaagtttcct
     2881 tgattgtctc gtgctcgcat gtaacctgtc tcagcgtcaa atagctggcg gtaattttgt
     2941 gaagcagcct tgtaggtttc agcgatttct atgttctcta gttttttggc acagctggcg
     3001 atacaaaagt cactataggc atagtctaga gtatggctaa cactttcgtg gtggtcggta
     3061 gagaggtaac ctagttcttg gtattgggct agtccgtggc ggccattgat gccgagaggg
     3121 tcggctttgc tggctgtttc gagcatggct tggaagagtt ctccttctag gtcaggggcc
     3181 atgtccttgc aggcgctatc tgcgataata ccgtctaaaa gtgtacctgg catcataccc
     3241 cgttcatctg gagccagcca ttttggaagg aaaccagtat cgcggtagct attgaggaaa
     3301 ccttctaaaa agcgttgata gtgctccggt atgataaggg caaagagggg gaaggtggtg
     3361 cggaaggtat cccagaaacc attgttgcta aagaggacac caggcttgac agtaccagta
     3421 gccagatcca tgtggatggc ttgccctgac tcatcaacct cataaaaagt ctgtgggaag
     3481 aggaagagtc tgtagaggca gtggtcaaag aaggttcggt cagcctctcc tgtctctata
     3541 acatcaaaac gatggaggag attttcccaa tccgcttggg cacttgcttt acagctatca
     3601 aaatcttctt gaggtagatt gattagagct tgagaaggag agatgaaaga agtggctagt
     3661 tgcatctcgg tttgactact tactaagtca attcgccagt ctcccccttc ttggctgata
     3721 gcaagaatat ccgtgttcat ttgtagggca gtgaacatcg ttagcgaatt tttgttggtt
     3781 tcagttttac cttcttgtcg cagggcaaga gttcgcttat ctacttgctc tactgtcagt
     3841 tcatctgctg cgtgaagata gagggagagg gctttgcctt gcttttgctt caaacgaata
     3901 gaagcaccgt agcaagtcgg tgtgagctgg gtttcaatct gatagcgcag ggagaaaatc
     3961 tttagataat gaggttggaa agaggcctta tccctatcat aggaagattg gcggtgaaag
     4021 agactgtctc ccccaagctg acctgtgaca ggtgtcagaa ggagccaaga gtagtcccca
     4081 atccaaggac tgggctggtg agttaatcga atcccctgaa agataggcag atgcggatca
     4141 aaaaaccaag atccatcctg gtcactggtc tggggcacaa agtaattcat cccaaaaggc
     4201 acgcctgtgt atggcagggt atttccccga gaaaaggcat gcttgttggt agttccaaag
     4261 cgggtatcga tggtttcaag tagtggtttc atagtctttc ctttagctgt ttttctacat
     4321 tatatcagaa aaagagggcc tttagaaaag gagtttcaaa aataactatt ttgtagaaaa
     4381 atgactatca tttgtgtatg tattttctgt ctcaaaagct atatactgaa catgaaactt
     4441 gtttgaaaga gggaattgca cgatgattta ttcgaaagaa attgttagag aatggctaga
     4501 tgaagtagca gagcgggcta aggaccatcc agagtgggtg gatgttttcg agcgttgcta
     4561 caccgatacc ttggacaata cggttgaaat cttagaagat ggttcaactt ttgtcttgac
     4621 tggggatatt cctgccatgt ggcttcgaga ttcgacagcc caactcagac cctaccttca
     4681 tgtagctaaa agagatgccc tcctgcgtca gaccattgca ggtttggtca aacgtcagat
     4741 gaccttggta ctcaaggatc cctatgctaa ctccttcaac attgagaaga actggaaagg
     4801 gcaccacgag actgaccaca cagaccttaa cggctggatc tgggagcgca agtatgaggt
     4861 ggattcactt tgctatcctt tgcagttggc ttatctcctc tggaaagaga ctggcgagac
     4921 tggtcagttt gatgagactt ttgtcgcagc gactaaggaa attctccatc tatggacagt
     4981 tgaacaagac cacaagaact ctccttatcg ttttgtccga gatacggacc gtaaggaaga
     5041 caccttggta aatgatggct ttggacctga ctttgcagtg acaggtatga cttggtcagc
     5101 ttttcgtccg agtgacgatt gctgtcagta tagttacttg attccgtcta atatgtttgc
     5161 cgtagtagtc ttgggttatg tgcaagaaat ctttgtcgaa ttagacctag ctgatagcca
     5221 gagtgttatt gctgatgcca agcgtcttca ggatgaaatc caagaaggaa tcgaaaacta
     5281 cgcttacacc accaacagca agggcgaaaa gatttacgct tttgaagtag atggcctagg
     5341 aaatgccagc atcatggatg atccaaatgt accaagttta ctagctgcgc cctatctggg
     5401 ctactgttcg gtcgatgatg aagtgtatca agctactcgt cgtaccattt tgagctctga
     5461 aaatccatat ttctaccaag gagaatacgc tagcggtctc ggaagttctc ataccttcta
     5521 tcgctatatc tggccaatcg ccctttctat ccaaggattg acaacaagag ataaggcaga
     5581 gaaaaaattc ttgctggatc agctggttgc ctgcgatggt ggtacaggtg tcatgcacga
     5641 aagcttccac gtggatgacc caactctcta ctctcgtgaa tggttctcct gggctaacat
     5701 gatgttctgt gagttggtct tggattactt ggatattcgc taaggggctc gctttagctc
     5761 aaccgattct tatcagaatc acaagtttac atttaaaacg ttaaaattta aatttagaat
     5821 gaggttttac ttcatggaaa atgttgttgt acatattatc tcacatagtc actgggaccg
     5881 tgagtggtac ttgccttttg aaagtcatcg tatgcagttg gtggaattgt ttgacaatct
     5941 ctttgatctc tttgaaaatg accctgagtt caagagtttc cacttggatg gacaaactat
     6001 tgtccttgac gactacttac aaattcgccc tgaaaatcgc gacaaggtcc aacgctacat
     6061 tgacgagggc aagcttaaaa ttggtccctt ttacatcttg caggatgatt atttgatttc
     6121 aagtgaagcc aatgtccgca ataccttgat tggtcaacaa gaagctgcca aatggggtaa
     6181 atcaacccag attggctact ttccagatac ctttggaaat atgggacaag cacctcaaat
     6241 tcttcaaaaa tcaggcattc atgtggcggc ctttggtcgt ggtgtgaagc cgattggatt
     6301 tgacaaccaa gtccttgaag atgagcagtt tacatcccag ttttcagaaa tgtactggca
     6361 gggtgtggat ggtagtcgtg ttttaggtat tctctttgcc aactggtaca gtaatgggaa
     6421 tgaaatccca gttgacaaag atgaggcctt gaccttctgg aaacaaaaat tgtcagatgt
     6481 gcgtgcctac gcttcgacca accaatggtt gatgatgaac ggctgtgacc accagcctgt
     6541 acagaaaaat ctgagcgaag ccattcgtgt ggcaaatgaa ctcttcccgg atgtaatctt
     6601 tgttcatagt tcttttgatg aatatgttca agctgtagaa ggtgcgcttc ctgaacactt
     6661 atcaactgtt acaggcgagt tgaccagtca ggaaacagat ggctggtaca cacttgccaa
     6721 cacttcttca tcccgcattt acctaaaaca agccttccaa gaaaatagca acctcctaga
     6781 gcaagtggta gaacccttga ctattatcac tggtggacac aaccacaagg accagttgac
     6841 ctatgcttgg aaaacacttt tgcagaacgc gccacatgat agtatctgtg gctgtagcgt
     6901 ggacgaagtt caccgcgaga tggaaacgcg ttttgccaag gtcaaccaag taggaaactt
     6961 tgttaaaagt aacttgctca acgagtggaa gggtaaaatt gctacggata aggctcaaag
     7021 tgactatctc tttactgtca ttaacacagg cttgcatgat aaggtcgata ctgtcagcac
     7081 agtgattgat gtggcgactt gtgatttcaa ggaattgcac ccaacagaag gctacaaaaa
     7141 gatggctgct cttatcttgc caagttaccg tgtggaggac ttggatggtc gtcctgtaga
     7201 ggctacaatc gaagacctcg gagctaattt tgagtacgat ttaccaaaag ataagttccg
     7261 ccaagctcgt attgctcgac aagtgcgcgt gaccattcca gttcacctag cgccgctttc
     7321 ttggacaacc ttccaattgc tggaaggaaa acaagaacac cgtgagggta tttaccaaaa
     7381 cggagtgatt gatacaccat tcgtaacggt gagtgtggat gacaacatca cagtctatga
     7441 caagacaact cacgaagcct atgaagactt tatccgcttt gaagaccggg gtgacatcgg
     7501 aaacgagtat atctatttcc aaccaaaagg aacagagcca atctttgcag agcttaagga
     7561 ccacgaggtc ttggaaaaca cagatcgcta tgctaaaatc ttgctcaaac atgaattgac
     7621 cgtgcctgtc agtgcggatg aaaagctaga agaagagcaa caaggtatca tcgagtttat
     7681 gaagcgtgag gctggacggt cagaagaatt gacaaacatt cctctggaaa ctgagttgac
     7741 tgtcttcgtt gacaatccac aaatccgctt caagactcgc tttactaaca ctgccaagga
     7801 tcaccgtatc cgtctcttgg tcaagactca taacacgcgt ccaagcaatg attctgaaag
     7861 tatctatgag gtggtgacac gaccaaacaa accagctgct tcatgggaaa accctgaaaa
     7921 tcctcaacac caacaagctt ttgtcagtct gtatgacgat gaaaaagggg tgactgtatc
     7981 caacaaggga ttgaatgaat acgaaatcct tggggataac accattgccg tgaccatttt
     8041 gcgtgcatca ggtgagctag gtgactgggg ctacttccca acgccagaag cacaatgctt
     8101 gcgggagttt gaagtcgagt ttgcacttga atgccaccaa gcccaagaac gcttctcagc
     8161 ctatcgtcgt gccaaagcct tgcagacacc gtttaccagc cttcagcttg ctagacagga
     8221 aggaagcgtg gttgcgactg gtagcctctt gagccattct gttctcagca taccgcaagt
     8281 ttgtccaaca gcctttaagg tagctgaaaa tgaagaaggc tatgtgcttc gttactacaa
     8341 tatgtgtagt gaaaatgtac gtgtgccaga aagtcaacat ctcttccttg acctacttga
     8401 acgaccatac ccagttcatt caggactatt ggctccacaa gagattcgta cagaattcat
     8461 caaaaaagaa gaaatttaat ttcaaaaagt aaacatcaaa agaaaggagg ggcgaaaaag
     8521 taagaactaa ctgctgattc gcccctttta tggtaaaaac aatgaccatt gcaacgattg
     8581 atatcggagg gactgggatt aagtttgcca gtctgactcc tgatgggaaa atactggata
     8641 agacaagtat ttcaacgcct gaaaacttgg aggatttact agcgtggcta gatcaacgct
     8701 tgtcagaaca ggattacagt gggattgcta tgagcgttcc aggtgcagtc aatcaagaga
     8761 caggtgtgat tgatggcttc agtgcggtgc cctacatcca tggcttttct tggtatgagg
     8821 cgcttagctc ttatcagcta cctgtccatt tagaaaatga tgccaactgc gttggactca
     8881 gtgaactact agctcatcca gagcttgaaa atgcagcctg tgtcgtgatt gggacaggga
     8941 ttggcggagc catgattatc aatggtagac ttcatcgagg tcgccacggt ctgggtggag
     9001 aatttggcta catgacaacc cttgcccctg ctgaaaaact taataactgg tcgcaactag
     9061 catcaactgg gaatatggta cgatacgtga ttgaaaaatc tggtcatact gattgggacg
     9121 gtcgcaagat ttaccaagag gccgcagctg gtaatatcct ttgtcaagaa gccattgagc
     9181 gcatgaaccg caatctggcg caaggcttgc tcaatatcca gtatctgatc gatccaggtg
     9241 tcatcagtct gggtggctct atcagtcaaa atccagattt tatccaaggt gtcaagaagg
     9301 ctgctgaaga ctttgtcgat gcctacgaag aatacacggt cgcaccagtt atccaggcct
     9361 gcacctatca cgcagatgcc aatctctacg gtgctcttgt caactggcta caggaggaaa
     9421 agcaatggta agatttacag gacttagtct caaacaaacg caagctattg aggttttaaa
     9481 aggtcacatt tctctaccag atgtggaagt ggctgtcact cagtctgacc aagcatctat
     9541 ctctatcgag ggtgaggaag gtcactatca attgacctac cgcaaacctc accaacttta
     9601 tcgtgccttg tccttgttgg taacagttct agcagaagct gataaagtag agattgagga
     9661 acaagcagct tacgaagatt tggcttacat ggttgactgt tctcgaaatg cggtgctgaa
     9721 tgtggcttct gccaagcaga tgattgagat attggctctc atgggctact caacctttga
     9781 gctttacatg gaagacactt accagattga agggcagcct tactttggct atttccgtgg
     9841 agcttattca gcagaggagt tgcaggaaat cgaagcctat gcccaacagt ttgacgtgac
     9901 ctttgtacca tgcatccaga ccttggccca cttgtcggcc tttgtcaaat ggggtgtcaa
     9961 ggaagtgcag gagctccgtg atgtagagga cattcttctc attggcgaag aaaaggttta
    10021 tgacttgatt gatggcatgt ttgccacgtt gtctaaactg aagactcgca aggtcaatat
    10081 cgggatggac gaagcccact tggttggttt gggacgctac ctgattctga acggtgttgt
    10141 ggatcgtagt ctcctcatgt gccaacactt ggagcgcgtg ctggatattg ctgacaaata
    10201 tggtttccac tgccagatgt ggagtgatat gttcttcaaa ctcatgtcag cggatggcca
    10261 gtacgaccgt gatgtggaaa ttccagagga aactcgtgtc tacctagacc gtctcaaaga
    10321 ccgtgtgact ctggtttact gggattatta tcaggatagc gaggaaaaat acaaccgtaa
    10381 tttccgcaat catcacaaga ttagccatga ccttgcattt gcagggggag cttggaagtg
    10441 gattggcttt acacctcaca accattttag ccgtctagtg gctatcgagg ctaataaagc
    10501 ctgccgtgcc aatcagatta aagaagtcat cgtaacgggt tggggagaca atggtggtga
    10561 aactgcccag ttctctatcc taccaagctt gcaaatctgg gcagaactca gctatcgcaa
    10621 tgacctagat ggtttgtctg cgcacttcaa gaccaatact ggtctaacgg ttgaggattt
    10681 tatgcagatt gaccttgcca acctcttacc agacctacca ggcaatctca gcggtatcaa
    10741 tcccaaccgc tatgtttttt atcaggatat tctttgtccg attcttgatc aacacatgac
    10801 acctgaacag gacaaaccgc acttcgctca ggctgctgag acgcttgcta acattaaaga
    10861 aaaagctgga aactatgcct atctctttga aactcaggcc cagttgaatg ctattttaag
    10921 tagcaaagta gatgtgggac gacgcattcg tcaggcctac caagcggatg ataaagaaag
    10981 tttacaacaa atcgccagac aagaattacc agaacttaga agccaaattg aagacttcca
    11041 tgccctcttt agccaccaat ggctgaaaga aaacaaggtc tttggtttgg atacagttga
    11101 catccgtatg ggcggactct tgcaacgcat caaacgagca gaaagccgta tcgaggttta
    11161 tctggctggt cagcttgacc gcatcgacga gctggaagtt gaaatcctac catttactga
    11221 cttctacgca gacaaggatt tcgcagcaac tacagccaac cagtggcata ccattgcgac
    11281 agcgtcgacg atttatacga cttaatattc ttcgaaaatc tcttcaaacc acgtcagctt
    11341 ccatctgcaa cctcaaaaca gtgttttgag caacctgcag ctagcttcct agtttgctct
    11401 ttgattttca ttgagtataa aaacaagaac accttgcttg gcgcagggtg tttcgcgtga
    11461 aacagaagaa ttatctggtt tcaaatgcta cagttagaca aacttatgat aaaatagcag
    11521 aaagtgaatg tttcctaaga gcaattggag gtattatgct acacttaaaa ttagtaaaac
    11581 aagaaataga agctgaaaag ccagcatctg tagaagcttg gatcatttcc gtcaaattta
    11641 aaaaaggttg ctaccgacat atatagattc caaaaacaaa aacgttagcg gaactagcag
    11701 atgtgatttt atggagtttt gattttgcaa atgatcatgc tcacgcattt ttcatggata
    11761 atgttgagtg gagtcatgca gattcttact ttcgtagctt tgttagtgac gatgttgaag
    11821 aacgttacac agaaaatgtc tatctggata gcctaagtgt caaacaaaaa tttaagttta
    11881 ttttcgactt cggtgatgaa tggcgttttg aatgccaagt gctgagagaa atcgagacag
    11941 aggacgaaga agcttatctc gtacgttcgg ttggaacgtc gccagaacaa tatccagatt
    12001 atgatggttt tgactatgaa gaatggtaaa attgaaatca gtctgtgtag gcttagtatt
    12061 tcaatagact tcctgcaaaa ctagaatcct agttcatgat tgataatacc agcaatcaaa
    12121 ttcattcgta atccgaagcg tttacgatga tttcgatagg ttgttgaaaa cattttaaac
    12181 gtttttactt tggcaaagat gttctcaacc ttgcttctct ccttagatag cgcat
//
LOCUS       DAVHEW010000058        12139 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVHEW010000058 DAVHEW010000000
VERSION     DAVHEW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.058"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..176
                     /locus_tag="VVL53_001433"
     CDS             <1..176
                     /locus_tag="VVL53_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU4183592.1"
                     /translation="LIQGGADPYGKNPNGRYDWEIEPVLTLPVHGFCPRGTYDLGYIG
                     GGNHLCKGSAARF"
     gene            230..901
                     /locus_tag="VVL53_001434"
     CDS             230..901
                     /locus_tag="VVL53_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183593.1"
                     /translation="MKKRAIQILLALSLIFYKSIWFWRVFNHLAKLYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISFNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(953..1150)
                     /locus_tag="VVL53_001435"
     CDS             complement(953..1150)
                     /locus_tag="VVL53_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183594.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1143..2051
                     /locus_tag="VVL53_001436"
     CDS             1143..2051
                     /locus_tag="VVL53_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU4183595.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2048..2509
                     /locus_tag="VVL53_001437"
     CDS             2048..2509
                     /locus_tag="VVL53_001437"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU4183596.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2499..3386
                     /locus_tag="VVL53_001438"
     CDS             2499..3386
                     /locus_tag="VVL53_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4183597.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3389..5272
                     /gene="cbpE"
                     /locus_tag="VVL53_001439"
                     /gene_synonym="pce"
     CDS             3389..5272
                     /gene="cbpE"
                     /locus_tag="VVL53_001439"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU4183598.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAETGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5352..6482
                     /locus_tag="VVL53_001440"
     CDS             5352..6482
                     /locus_tag="VVL53_001440"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU4183599.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQYGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6492..7754
                     /locus_tag="VVL53_001441"
     CDS             6492..7754
                     /locus_tag="VVL53_001441"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU4183600.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASFLPRLEQ
                     VLVAERKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7758..8555
                     /gene="proC"
                     /locus_tag="VVL53_001442"
     CDS             7758..8555
                     /gene="proC"
                     /locus_tag="VVL53_001442"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU4183601.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8671..9309
                     /locus_tag="VVL53_001443"
     CDS             8671..9309
                     /locus_tag="VVL53_001443"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU4183602.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9306..10196
                     /locus_tag="VVL53_001444"
     CDS             9306..10196
                     /locus_tag="VVL53_001444"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU4183603.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFESLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10236..10553
                     /gene="yabA"
                     /locus_tag="VVL53_001445"
     CDS             10236..10553
                     /gene="yabA"
                     /locus_tag="VVL53_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU4183604.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10556..11425
                     /gene="rsmI"
                     /locus_tag="VVL53_001446"
     CDS             10556..11425
                     /gene="rsmI"
                     /locus_tag="VVL53_001446"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU4183605.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11652..>12139
                     /locus_tag="VVL53_001447"
     CDS             11652..>12139
                     /locus_tag="VVL53_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU4183606.1"
                     /translation="MRTYENKEELKAEIEKTFEKYISEFDNIPENLKDKRVDEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RT"
BASE COUNT         3627 a         2084 c         2902 g         3526 t
ORIGIN      
        1 agctgataca aggtggagca gatccatatg gtaaaaatcc taatggtagg tacgattggg
       61 aaatagaacc agtattaact ctgccggttc atggattttg tcccagaggc acctatgatt
      121 taggatatat tggaggaggc aatcatcttt gcaaaggaag tgctgcgaga ttttaagtaa
      181 aatttattag gaatatgaag aaacaagtgg agagagcaga gaatttaata tgaaaaaacg
      241 ggctattcaa attttactag cattgtcctt aattttttac aaatcaattt ggttttggag
      301 agttttcaat cacctcgcaa agctctatct accagcaagt cgtgaatttt ttcagattct
      361 gcttttgatg gagagcggag ttcttttctt agcggtcatc tatctactgg tttttgcagg
      421 aaagaaaatt tttcatttca agtggcagct gaggtacttc atctaccttt tactgggcta
      481 catcatttca tatatgtctg acttcctctt ttcgtatttc atatccctgt cttcaaatca
      541 gatttctttt aatgaaacgg tagaaatgat ggggagacag gagttccctt atttcttgct
      601 catcgtttgc ttcatcgccc ctattgctga ggaattgatt tatcgaggtg tgcttatgac
      661 aacctttttc aaaaactcac cttggtacgg agatgttttg ctttctgcta ttattttcgg
      721 ttatattcat atcaattttg ctttaactcc tcttgctttt ttcatttatg ctagtggagg
      781 tcttatttta gctctattgt atcgcatgac taaaaatctc tactatccaa tactagttca
      841 tattctcatc aatatcactg ccttctggga tgtttggtta ctcctatttt caggaagtta
      901 gcttactaaa aaaatgtcgg aattttccgg cattttcttt tttcacaaat agtcaacgtt
      961 tttctttccg atactgaagt ggtgtgtagc cacttatttt tttgaattga ttttgaaaat
     1021 aagattggcg tgagaaaggc agatagtgaa gatagttaag aagaatagga tgttcttttt
     1081 tcctttttgg aaaacttcta aaatatggta taatgaaaag ataaagaagt tgggggtaga
     1141 agatgaacat tcaacaatta cgctatgttg tggctattgc caatagtggt acttttcgtg
     1201 aagctgctga aaagatgtat gttagtcagc cgagtctgtc tatttctgtt cgtgatttgg
     1261 aaaaagagtt gggctttaag attttccgtc ggaccagctc agggactttc ttgacccgtc
     1321 gtgggatgga attttatgaa aaagcgcaag aattggttaa aggatttgat atttttcaaa
     1381 atcagtatgc caatcctgaa gaagaaaaag atgaattttc cgttgctagc cagcactatg
     1441 acttcttacc accaactatt acggcctttt cagagcgcta tcctgactat aagaacttcc
     1501 gtatttttga atcaactact gttcaaatat tagatgaagt ggcgcaaggg catagtgaga
     1561 ttgggattat ctacctcaac aatcaaaata aaaaggggat tatgcaacgg gttgaaaagt
     1621 taggtctgga ggtcatcgaa ttgattcctt tccataccca tatttatctc tgtgagggtc
     1681 atcctttagc ccagaaagag gaattagtca tggaggattt agcggattta ccaacggttc
     1741 gtttcactca agagaaagac gagtaccttt attattcaga gaactttgtt gataccagcg
     1801 ctagctcaca gatgtttaat gtgacagacc gtgccacctt gaatggtatt ttggagcgga
     1861 cggacgccta tgcgacaggt tctggatttt tagatagtga cagtgttaat ggcattacag
     1921 ttattcgtct caaggataac ctagataacc gcatggtcta tgttaaacgt gaagaagtgg
     1981 agcttagtca agctgggact ctcttcgtag aagtcatgca agaatatttt gatcaaaaga
     2041 ggaaatcatg aaaaaaagag caatagtggc agtcattgta ctgcttttga ttgggctgga
     2101 tcagttggtc aaatcctata tcgtccagca gattccactg ggtgaagtgc gctcctggat
     2161 ccccaatttc gttagcttga cctacctgca aaatcgaggt gcagcctttt ctatcttaca
     2221 agatcagcag ctgttattcg ctgtcattac tctggttgtc gtgataggtg ccatttggta
     2281 tttacataaa cacatggagg actcattctg gatggtcttg ggtttgactc taataatcgc
     2341 gggtggtctt ggaaacttta ttgacagggt cagtcagggc tttgttgtgg atatgttcca
     2401 ccttgacttt atcaactttg caattttcaa tgtggcagat agctatctga cggttggagt
     2461 gattatttta ttgattgcaa tgctaaaaga ggaaataaat ggaaattaaa attgaaactg
     2521 gtggtctgcg tttggataag gctttgtcag atttgtcaga attatcacgt agtctcgcga
     2581 atgaacaaat taaatcaggc caggtcttgg tcaatggtca agtcaagaaa gctaaataca
     2641 cagtccaaga gggtgatgtc gtcacttacc atgtgccaga accagaggta ttagagtatg
     2701 tggctgagga tcttccgcta gaaatagtct accaagatga ggatgtggct gtcgttaaca
     2761 aacctcaggg aatggttgtg cacccgagtg ctggtcatac cagtggaacc ctagtaaatg
     2821 ccctcatgta tcatattaag gacttgtcgg gtatcaatgg ggttttgcgt ccagggattg
     2881 ttcaccgtat tgataaggat acgtcaggtc ttctcatgat tgctaaaaac gatgatgcgc
     2941 atctagcact tgcccaagaa ctcaaggata aaaagtctct ccgcaaatat tgggcgattg
     3001 ttcatggaaa tctgcctaat gatcgtggtg taattgaagc gccgattggc cggagtgaaa
     3061 aagaccgtaa gaaacaggct gtaactgcta aagggaagcc tgcagtgacg cgttttcacg
     3121 tcttggaacg ctttggcgat tatagcttag tagagttgca actggagaca gggcgcactc
     3181 atcaaatccg tgtccacatg gcttatatcg gccatccagt cgctggtgat gaggtctatg
     3241 gtcctcgcaa gactttgaaa ggacatggac aatttcttca tgccaagact ttaggtttta
     3301 ctcatccgag aacaggtaag accttggaat ttaaagcaga tatcccagag atttttaagg
     3361 aaaccttgga gagattgaga aagtaagaat gaaaaagaaa ttaactagtt tagcacttgt
     3421 aggcgctttt ttaggtttgt catggtatgg gaacgttcag gctcaagaaa gttcaggaaa
     3481 taaaatccac tttatcaatg ttcaagaagg tggcagtgat gcgattattc ttgaaagcaa
     3541 tggacatttt gccatggtgg atacaggaga agattatgat ttcccagatg gaagtgattc
     3601 tcgctatcca tggagagaag gaattgaaac gtcttataag catgttctaa cagaccgtgt
     3661 ctttcgtcgt ttgaaggaat tgggtgtcca aaaacttgat tttattttgg tgacccatac
     3721 ccacagtgat catattggaa atgttgatga attactgtct acctatccag ttgaccgagt
     3781 ctatcttaag aaatatagtg atagtcgtat tactaattct gaacgtctat gggataatct
     3841 gtatggctat gataaggttt tacagactgc tgcagaaaca ggtgtttcag ttattcaaaa
     3901 tataacacaa ggggatgctc attttcagtt tggggacatg gatattcagc tctataatta
     3961 tgaaaatgaa actgattcat cgggtgaatt aaagaaaatt tgggatgaca attccaattc
     4021 cttgattagc gtggtgaaag tcaatggcaa gaaaatttac cttgggggcg atttagataa
     4081 tgttcatgga gcagaagaca agtatggtcc tctcattgga aaagttgatt tgatgaagtt
     4141 taatcatcac catgatacca acaaatcaaa taccaaggat ttcattaaaa atttgagtcc
     4201 gagtttgatt gttcaaactt cggatagtct accttggaaa aatggtgttg atagtgagta
     4261 tgttaattgg ctcaaagaac gaggaattga gagaatcaac gcagccagca aagactatga
     4321 tgcgacagtt tttgatattc gaaaagacgg ttttgtcaat atttcaacat cctacaagcc
     4381 gattccaagt tttcaagctg gttggcataa gagttcatat ggaaactggt ggtatcaagc
     4441 gcctgattct acaggagaat atgctgtcgg ttggaatgaa attaagggtg aatggtatta
     4501 ctttaaccaa acgggtatct tattacagaa tcaatggaaa aaatggaaca atcgttggtt
     4561 ctatttgaca gactctggtg ttgctgctaa aaattggaag aaaatcgctg gaatctggta
     4621 ttattttaac aaagaaaacc agatggaaat tggttgggtt caagataaag agcagtggta
     4681 ttatttggat gttgatggtt ctatgaagac aggatggctt caatatatgg ggcaatggta
     4741 ttactttgct ccatcagggg aaatgaaaat gggctgggta aaagataaag aaacctggta
     4801 ctatatggat tctactggtg tcatgaagac aggtgagata gaagttgctg gtcaacatta
     4861 ttatctggaa gattcaggag ctatgaagca aggctggcat aaaaaggcaa atgattggta
     4921 tttctacaag acagacggtt cacgagctgt gggttggatc aaggacaagg ataaatggta
     4981 cttcttgaaa gaaaatggtc aattacttgt gaatggtaag acaccagaag gctatactgt
     5041 ggattcaagt ggtgcctggt tagtggatgt tccgatcgag aaatctgcta caattaaaac
     5101 tacaagtcat tcagaaataa aagaatccaa agaagtagtg aaaaaggatc ttgaaaataa
     5161 agaaacgagt caacatgaaa gtgttacaaa ttcttcaact agtcaagatt tgacatcctc
     5221 aacttcacaa agctctgaaa cgagtgtaaa caaatcggaa tcagaacagt agtagaaaag
     5281 aaggttttag ggccttcttt ttcctatcaa ctcttttcta tttcctgtta ttcatgttat
     5341 aatggataaa tatgaataat cggagtgaga ctatgaaata caaacggatt gtctttaagg
     5401 tgggtacttc ttctctgaca aatgaggatg gaagtttatc acgtagtaag gtaaaggata
     5461 ttacccagca gttggctatg ctgcacgagg ctggtcatga gttgattttg gtgtcttcag
     5521 gtgccattgc ggctggtttt ggagccttag gatttaaaaa gcgtccgact aagattgctg
     5581 ataaacaggc ttcagcagcg gtagggcagg ggcttttgtt ggaagaatat acaaccaatc
     5641 ttctcttgcg tcaaatcgtt tctgcacaaa tcttgctgac ccaagatgac tttgtggata
     5701 agcgtcgtta taaaaatgcc catcaggctt tgtcggtttt gctcaaccgt ggggcaattc
     5761 ctatcatcaa tgagaatgat agtgtcgtta ttgatgagct caaggttggg gacaatgaca
     5821 ctctaagtgc tcaagtagcg gcgatggtcc aagcagacct tttagttttc ttgacagatg
     5881 tggacggtct ctatactgga aatcctaatt cagatccaag agccaaacgc ttggagagaa
     5941 tcgagaccat caatcgtgag attattgata tggctggtgg agctggttcg tcaaacggaa
     6001 ctgggggtat gttaaccaaa atcaaggctg caactatcgc gacggaatca ggagttcctg
     6061 tttatatctg ctcatccttg aaatcagatt ccatgattga ggcggcagag gagaccgagg
     6121 atggttctta ctttgttgct caagagaagg ggcttcgtac ccagaaacaa tggcttgcct
     6181 tctatgctca gagtcaaggt tctatttggg ttgataaagg ggctgcggaa gctctctctc
     6241 aatatggaaa gagtcttctc ttatctggta tcgttgaagc agaaggagtc ttttcttacg
     6301 gtgatatcgt gacagtattt gacaaggaaa gtggaaaatc acttggaaaa ggacgcgtgc
     6361 aatttggagc atctgctttg gaggatatgt tgcgttctca aaaagccaag ggtgtcttga
     6421 tttaccgtga cgactggatt tccattactc ctgaaatcca actacttttt acagaatttt
     6481 agaggtaaac tatggtgagt agacaagaac aatttgaaca ggtacaggct gttaaaaaat
     6541 cgattaacac agctagtgaa gaagtgaaaa accaagcctt gctagccatg gctgatcact
     6601 tagtggctgc tactgaggaa attttagcgg ctaatgccct cgatatggca gcggctaagg
     6661 ggaaaatctc agatgtgatg ttggatcgtc tttatttgga tgcagatcgt atagaagcga
     6721 tggcaagagg aattcgtgaa gtggttgcct taccagatcc aatcggtgaa gttttagaaa
     6781 caagtcagct tgaaaatggt ttggttatca caaaaaaacg tgtagctatg ggtgtcatcg
     6841 gtattatcta tgaaagccgt ccaaatgtga cgtctgatgc ggctgctttg actcttaaga
     6901 gtggaaatgc ggttgttctt cgtagtggta aggatgccta tcaaacaacc catgccattg
     6961 tcacagcctt gaagaagggc ttggagacga ctactattca tccaaatgtg attcaactgg
     7021 tggaggatac tagccgtgaa agtagttatg ctatgatgaa ggccaagggc tatctagacc
     7081 ttctcattcc tcgtggagga gctggcttga tcaatgcagt ggttgagaat gcgattgtac
     7141 ctgttatcga gacagggact gggattgtcc atgtctatgt ggataaggat gcagacgaag
     7201 acaaggcgct gtctatcatc aacaatgcta aaaccagtcg tccttctgtt tgtaatgcca
     7261 tggaggttct gctggttcat gaaaacaagg cagcaagctt ccttcctcgc ttggagcaag
     7321 tgttggttgc agagcgtaag gaagctggac tggaaccaat tcaattccgc ctagatagca
     7381 aagcaagcca gtttgtttca ggtcaagctg ctcaagcaca agactttgat accgagtttt
     7441 tagactatat tctagctgtt aaggttgtga gcagtttaga agaagcggtt gcgcatattg
     7501 aatcccacag tacccatcat tcggatgcca ttgtgacgga aaatgctgaa gctgcagcat
     7561 actttacaga tcaagtggac tctgcagcgg tgtatgttaa tgcctcaact cgtttcacag
     7621 atggaggaca atttggtctt ggttgtgaaa tggggatttc tactcagaaa ttgcacgcgc
     7681 gtggtccaat gggcttgaaa gagttgacca gctacaagta tgtggttgct ggtgatgggc
     7741 agataaggga gtaagagatg aagattggat ttatcggttt ggggaatatg ggtgctagct
     7801 tggcaaaatc tgtcttgcag actaggacgt cagatgagat tctccttgcc aatcgtagtc
     7861 aagctaaggt agatgctttc attgcagact ttggtggtca ggcttccagc aatgaagaaa
     7921 tgtttgcaga agcagatgtg atttttctag gagttaagcc tgctcagttt tctgaactgc
     7981 tttctcaata ccagaccatc cttgaaaaaa gagaaagtct tcttttgatt tcgatggcag
     8041 ctggattgac cttagaaaaa ctagcaagtc ttatcccaag tcaacaccga attattcgta
     8101 tgatgcctaa tacccctgct tctatcgggc aaggagtgat tagttatgcc ttgtctccta
     8161 attgcagggc tgaggacagt gagctctttt gtcagctttt atccaaggct ggtctcttgg
     8221 ttgaattagg agaaagctta atcaatgcag cgacaggtct tgcaggctgt ggaccagcct
     8281 ttgtctatct ctttattgaa gccttggcag atgcaggtgt tcagacggga ttatcacgag
     8341 aaacagcctt gaaaatggca gcccaaactg tggtaggagc tgggcaattg gtcctagaaa
     8401 gccagcaaca tcctggagtt ttgaaagatc aagtttgtag tccaggcggt tcgactattg
     8461 ctggcgtagc aagcctagaa gcgcatgctt ttcgaggcac agtcatggag gcagttcatc
     8521 aagcctataa acgaacacaa gaactaggta aataagaggt agctttgact gcctctttta
     8581 tggtggctga aatgagaaga cacaaaaaga ttgtcacaaa cccctatttt tttgatagaa
     8641 tagaagtagt aaaaaagaaa tgagttagac atgtcaaaag gatttttagt ctctcttgag
     8701 ggaccagagg gagcaggcaa gaccagtgtt ttagaggctc tgctaccaat tttagaggaa
     8761 aaaggagtag aggtgttgac gacccgtgaa cctggcggag tcttgattgg ggagaagatt
     8821 cgggaagtga ttttggatcc aagtcatact cagatggatg ctaaaacaga gctacttctc
     8881 tatattgcca gtcgcagaca gcatttggtg gaaaaagttc ttccagccct tgaagctggc
     8941 aagttggtca tcatggatcg ttttatcgat agttctgttg cctatcaggg atttggtcgt
     9001 ggcttagata ttgaagccat tgactggctc aatcagtttg cgacagatgg cctcaaaccc
     9061 gatttgacac tctattttga catcgaggtg gaagaagggc tggctcgtat tgctgctaat
     9121 agtgaccgcg aggttaatcg tttggatttg gaagggttgg acttgcataa aaaagttcgt
     9181 caaggctacc tttctcttct ggataaagag ggaaatcgca ttgtcaagat tgatgctagt
     9241 ctccctttgg agcaagttgt ggaaactacc aaggctgtct tgtttgacgg aatgggcttg
     9301 gccaaatgaa acaagatcaa ctaaaggctt ggcaaccagc tcagtttgac cgttttgtcc
     9361 gtatcttaga acaagaccag ctcaatcacg cctatctctt ttcaggtttc tttgaaagct
     9421 tggaaatggc gcaattttta gctaagagcc tcttttgtac ggataaagtt ggcgtcttac
     9481 catgtgagaa atgccgaagt tgcaagctga ttgaacaggg agaatttccc gatgtcacct
     9541 tgattaaacc agttaatcag gtcattaaga cggaacgcat tcgagaattg gtgggtcagt
     9601 tttctcaagc agggattgaa agccagcaac aggtctttat catcgagcaa gcggataaaa
     9661 tgcatcccaa cgcagccaat tctctgctca aggtcatcga agaaccccag agtgaagttt
     9721 atattttctt cttgactagc gatgaggaaa agatgttacc gacaatccga agtcggactc
     9781 agatcttcca ctttaaaaag caagaagaaa aacttatctt actcttagaa caaatgggac
     9841 ttgttaagaa aaaagcgact cttttagcta agtttagtca atcgcgagct gaagcagaaa
     9901 agttggctaa tcaggcaagt ttttggacct tggtcgatga aagtgaacgc ctgctgactt
     9961 ggttagtagc taagaaaaaa gaaagttatc tacaggttgc caaattagcc aacttggcag
    10021 atgataagga aaaacaggat caggttttac ggattcttga agttctctgt gggcaggacc
    10081 tcttgcaggt aagagtaaga gtgattctac aagatttact agaagctaga aaaatgtggc
    10141 aagctaatgt cagctttcaa aatgccatgg aatatctggt cttgaaagaa atataaactc
    10201 aaaaatgaat gataaagaaa ggaaagggct gttttatgga caaaaaagaa ttatttgacg
    10261 cgctggatga tttttcccaa caattattgg taaccttagc cgatgtggaa gccatcaaga
    10321 aaaatctcaa gagcctggta gaggaaaata cagctcttcg cttggaaaat agtaagttgc
    10381 gagaacgctt gggtgaggtg gaagcagatg ctcctgtcaa ggccaagcat gttcgtgaaa
    10441 gtgtccgtcg catttaccgt gatggatttc acgtatgtaa tgatttttat ggacaacgtc
    10501 gagagcagga cgaggaatgt atgttttgtg acgagttgct atacagggag taggcatgca
    10561 gattcaaaaa agttttaagg ggcagtctcc ctatggcaag ctgtatctag tggcaacgcc
    10621 gattggcaat ctagatgata tgacctttcg agctatccag accttgaaag aagtagattg
    10681 gattgctgct gaggatacgc gcaatacagg tcttttgctc aagcattttg acatttccac
    10741 caagcagatc agttttcatg agcacaatgc caaggaaaaa attcctgatt tgattggttt
    10801 cttgaaagca gggcaaagta ttgctcaggt ctctgatgcc ggtttgccta gcatttcaga
    10861 ccctggtcat gatttagtta aggcagctat tgaggaagaa attgcagttg tgacagttcc
    10921 aggtgcctct gcaggaattt ctgccttgat tgccagtggt ttagcgccac agccacatat
    10981 cttttacggt tttttaccga gaaaatcagg tcagcagaag caattttttg gcttgaaaaa
    11041 agattatcct gaaacacaga ttttttatga atcacctcat cgtgtagcag acacgttgga
    11101 aaatatgtta gaagtctacg gtgaccgctc agttgtcttg gtcagggaat tgaccaaaat
    11161 ctatgaagaa taccaacgag gtactatctc tgagttatta gaaagcattg ctgaaacgcc
    11221 actcaagggc gaatgtcttc tcattgttga gggtgccagt cagggtgtgg aggaaaagga
    11281 cgaggaagac ttgttcgtag aaattcaaac ccgcatccag caaggtgtga agaaaaacca
    11341 agctatcaag gaagtcgcta agatttacca gtggaataaa agtcagctct acgctgccta
    11401 ccacgactgg gaagaaaaac aataaaggga gacaggatgt aataattctg tctgtttctg
    11461 tttaacttaa ttagtgatga taatataaag atgtatcact tggtatagaa gctttggtat
    11521 taagtttttt attaagccca tacggaatac cgatggttgg agcagcagtt atagcgttcc
    11581 tagaaggtat aaatagaaaa ataaggtcat tttaaatcaa aggattgata aatcagaaag
    11641 aaggtgattt tttgcgaaca tacgaaaata aagaagaact aaaagctgag atagagaaaa
    11701 catttgagaa atatatttca gaatttgata atattccaga aaatttaaaa gataagagag
    11761 ttgatgaagt tgatagaact ccagcagaaa accttgctta tcaggttggc tggaccaact
    11821 tggttcttaa atgggaagaa gatgaaagaa aggggcttca agtaaaaaca ccatcggata
    11881 aatttaaatg gaatcaactt ggtgaattat atcagtggtt cacagatacc tacgctcatt
    11941 tatctctgca agagttgaaa gcaaaattaa atgaaaatat taattctatc tctgcaatga
    12001 ttgattcgtt gagtgaggaa gaattatttg aaccgcatat gagaaagtgg gctgatgaag
    12061 cgactaaaac agcgacttgg gaagtgtata agtttattca tgtaaatacg gttgcacctt
    12121 ttggaacttt cagaactaa
//
LOCUS       DAVHEW010000059        12003 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVHEW010000059 DAVHEW010000000
VERSION     DAVHEW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.059"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            259..912
                     /locus_tag="VVL53_001448"
     CDS             259..912
                     /locus_tag="VVL53_001448"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4183607.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            922..1386
                     /locus_tag="VVL53_001449"
     CDS             922..1386
                     /locus_tag="VVL53_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU4183608.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1399..2667
                     /locus_tag="VVL53_001450"
     CDS             1399..2667
                     /locus_tag="VVL53_001450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU4183609.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2681..2941
                     /locus_tag="VVL53_001451"
     CDS             2681..2941
                     /locus_tag="VVL53_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183610.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2952..4313
                     /gene="pdrM"
                     /locus_tag="VVL53_001452"
     CDS             2952..4313
                     /gene="pdrM"
                     /locus_tag="VVL53_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU4183611.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4467..5435
                     /locus_tag="VVL53_001453"
     CDS             4467..5435
                     /locus_tag="VVL53_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU4183612.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5451..6443
                     /locus_tag="VVL53_001454"
     CDS             5451..6443
                     /locus_tag="VVL53_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU4183613.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVAYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6661..7704
                     /locus_tag="VVL53_001455"
     CDS             6661..7704
                     /locus_tag="VVL53_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU4183614.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7768..9453
                     /gene="lpdA"
                     /locus_tag="VVL53_001456"
     CDS             7768..9453
                     /gene="lpdA"
                     /locus_tag="VVL53_001456"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU4183615.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVNKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9517..10506
                     /locus_tag="VVL53_001457"
     CDS             9517..10506
                     /locus_tag="VVL53_001457"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU4183616.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10571..11641)
                     /gene="xerS"
                     /locus_tag="VVL53_001458"
     CDS             complement(10571..11641)
                     /gene="xerS"
                     /locus_tag="VVL53_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU4183617.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGCKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3573 a         2230 c         2681 g         3519 t
ORIGIN      
        1 ttctaaagaa actccttttt ctgagatgga ttttaatatg tttaaaaata tttcagaaaa
       61 gataatcttc gaaagaggta gtaaaatgct taatgattta aaaagtaatt ataaaaaata
      121 gccagtcttt ataaactgta ttcacgtatt caaatcgtat atatagtcga tttattaaat
      181 ctttagataa actgtaaaaa gtggtttcca tagccacttt ttgctataat agaggcagta
      241 aaaatgaagg agtcggctat ggaacactcg tcttggcatg ctttgattaa ggcgcaatta
      301 cctgagggtt atttcgggaa aatcaatcag tttatggagc aggtctattc tcaggggatt
      361 atttatccac ccaaggaaaa ggtttttcag gctctcttga caacactgct tgaagaagtt
      421 aaggtggtaa ttctagggca agacccctat cacggaccag gtcaagcgca gggcttgagt
      481 ttttctgtac ctgactctat cccagctcca ccatccttgc aaaatatctt gaaagaattg
      541 tcagatgata tcggagttaa gaaatctcat gatttgacag cttgggctga gcaaggagtc
      601 ttgcttctta atgcttgttt gactgttcct gctggacagg ccaatggtca tgctggtcaa
      661 atctgggagc cttttactga tgctgtgatt caggtggtca atcatctaga tagaccagtc
      721 gtttttgtac tctggggagc ttatgcacgt aagaagaagg cattagttac caatcctcat
      781 cacttgatta tcgaatcagc ccatccaagt cctttgtcgg tttatagagg attttggggt
      841 tccaagccct tttccaaggc caatgcattc ttaaaagaga caggacaaga gccaatcgat
      901 tggcttagat aaggagaaaa tatgcctcag ttagcgacga tttgctacat tgataatggg
      961 aaagaactgc tcatgctcca tcgtaataag aaacccaatg atgtccatga agggaaatgg
     1021 attggtgtgg gtggtaagct agagagagga gagacgcccc aggaatgcgc ggcgcgtgaa
     1081 atccttgaag aaacagggct caaagccaag ccagttctaa aaggtgtcat cacttttcct
     1141 gaatttacac cagatttaga ctggtacacc tatgttttta aggtgacgga gtttgagggc
     1201 gacttgattg actgcaatga ggggacgcta gaatgggttc cctatgatga ggttttgagc
     1261 aagccgactt gggaaggtga ccacaccttt gttgagtggc ttttagagga taaacccttc
     1321 ttttcagcca agtttgttta tgatggggat aaattgttgg atacccaagt tgatttctat
     1381 gaataaagga gaaagcagat gctactaatc aaaaatggtc gtgtaatgga tcccaagtct
     1441 ggtttggatc aagtttgtga tgtcttagtt caagatggga aaattatcaa aattgcgtct
     1501 gagatcacgg aagaaggagc agaaacgatt gatgctactg gtcttgtggt tgctcctggc
     1561 ttggtcgata ttcatgttca tttccgtgaa cctggtcaaa cacataaaga agacattcat
     1621 actggtgccc tagcagccgc tgcaggtggt tttactactg tcgtcatgat ggctaatacc
     1681 agtccaacca tttcagacgt ggagactttg caagcagttc tccagtcagc tgccaaagag
     1741 aagattaatg tcaagacagt tgcgaccatt actaaaaact ttaatggtaa aaacttgact
     1801 gactttaagg cactcttaga agctggtgcg gttggtttct ctgatgacgg tattccgctt
     1861 gagagcagta agattgtcaa ggaagccatg gaggaagcca aaaaactcaa tacctttatc
     1921 agccttcatg aggaagatcc aggtttgaac ggtgttcttg gctttaatga aaatattgct
     1981 agagaacatt tccatatctg cggtgctact ggggtggctg agtacgctat gatggcgcgt
     2041 gatgtcatga ttgcctatgc aactaaagcc catgttcaca tccagcattt gtctaaggaa
     2101 gaaagtgtta aagtagtgga gtttgctcag gggttaggtg cagaagtcac agcagaagta
     2161 gcgccacagc atttctctaa gacagaagca cttcttttaa cacaaggtag caatgctaag
     2221 atgaatccac cgcttcgttt ggaatcagac cgtcgtgccg ttatcgaagg tctcaaatca
     2281 ggtgtcatca cagttattgc gactgaccac gcgcctcatc atgtagatga aaaaaatgtt
     2341 gaggatatta ccaaagcgcc atctggtatg actggcttag aaacatccct gtctctcagc
     2401 ttgacctatt tagtagaagc tggtgagttg agcttgatgg aattacttga aaaaatgaca
     2461 tacaacccag ccaagcttta caactttgaa gcaggttact tggctgagaa tggtccagca
     2521 gatatcacta tttttgatgc caaggctgac cgctttgtgg actcccattt tgcttccaaa
     2581 gcagctaatt caccattcat cggtgaaacc ttaaaagggc aggttaaata taccatctgt
     2641 aagggacaaa tcgtctatca agcttgatag gagttagaat atgaattatt ttcgaaaacg
     2701 tagggagaga caagcgaaat ctaatagtgg aatttactgt cccgcagcta atcgttccaa
     2761 aattcaatat gaaactaaag aaaaagctga ccatgcggtt caatatgata acggaggttt
     2821 agttagaagc tattattgtc gtacatgtgc gtgttggcat actacttcaa aatctaataa
     2881 acctccgcta agtttgaaaa actatggtct gaaactcttt ggtttagata aatcagaata
     2941 gaaagagaag tatgtataag acaaagtgtt tacgagagaa gttagtatta tttttaaaaa
     3001 ttttcttccc aatcctgatc taccaatttg ccaattattc tgcctctttt gttgatactg
     3061 caatgacagg tcaatacaac actatggact tggctggtgt atctatggca accagtatct
     3121 ggaatccttt ctttacattt ctaacaggga ttgtgtcagc cttggtgcct atcattggtc
     3181 accatcttgg tcgaggcaaa aaggaagaag ttgcgtctga tttttaccaa tttatttatt
     3241 tggccttggg cctatctgtg gtcttgctgg ggatggtact tttcttggca ccaataatct
     3301 tgaatcatat tgggttagaa gcagcagtag cggcagtagc ggttcgctat ctttggtttt
     3361 tatctatcgg gattatcccc ttgttgctct ttagcgtcat tcgttccttg ctggattcgc
     3421 tgggcttgac caaactgtcc atgtacctca tgcttttgtt actccctctc aatagcggat
     3481 ttaactatct cttgatttac ggtgcctttg gtgttccaga actgggaggg gctggtgctg
     3541 gtttaggaac atccttggcc tactgggtct tgcttgggat ttctgttctg gttttattta
     3601 aacaggagaa gctcaaagcc ttacaccttg agaaacgaat tccacttaat atggataaaa
     3661 ttaaggaagg agttcgttta ggtctgccta ttgggggaac tgtcttcgcg gaagtggcta
     3721 tcttttcagt ggttggcttg attatggcta agttttcgcc cttgattata gctagtcacc
     3781 agtcagctat gaacttttca agtcttatgt acgcctttcc tatgagtatc tcatcggcta
     3841 tggctattgt cgtttcctat gaagtgggag ccaagcgatt tgatgatgcg aaaacctata
     3901 ttggtctagg aagatggact gccctcattt ttgcggcctt caccttaacc ttcctttaca
     3961 tttttagggg aaatgtggcc agtctttatg gtaacgaccc aaaatttatc gatttgacag
     4021 tgcgtttttt aacttatagt cttttcttcc agttagcaga tacctttgcg gcgccgcttc
     4081 agggaatttt gcgggggtat aaggatacag ttattccttt ttaccttggt ttgcttggtt
     4141 attggggcgt agcaatccct gtggctacgc tatttgattc cctaacagat tttggagctt
     4201 attcttactg gatcggcttg attattagtt tgattgtgag cggggcgctc taccgttggc
     4261 gtttaactgt gattatgaag agatttgaat ctttagcaaa atccaaatgc taaaaagttt
     4321 gtgaaaaaat attcttgaca agaaaatccc ttgttttcac ggggttttct tttttatttt
     4381 gtgaaatttc ttttagaaaa actttgacag tatttgccaa aagcggtaaa atagtaaggt
     4441 aagaagaaag ttagaaaggc aagaagatgt caactttaga taaaaatctt ttgctagaaa
     4501 tgttccgtaa gatggaagaa atccgtcgca tggacttaaa aattgcacaa ttagtaaaga
     4561 aagggaaagt gccaggaatg acgcactttt ctgttggtga agaggcagct aacgtggggg
     4621 ctatgttagc tctcaatcca gatgatctga ttacctcaaa ccaccgtgga cacgggcaag
     4681 ctattgctaa agggattgac ctcaacggaa tgatggctga aatccttggt aaatacactg
     4741 gaacctgtaa agggaaaggt ggatctatgc atatcgctga ccttgatgct gggaaccttg
     4801 gtgccaatgg tatcgtaggt ggtggtatgg ggatcgctgt cggtgcagcc ctcagtcagc
     4861 aaatgcaaaa taccggtaaa atcgttgtct gcttctttgg agatggtgcg accaatgaag
     4921 gtgttttcca cgaagcagtg aacatggctt ctatctggaa cctgccagtc attttctatt
     4981 gcattaacaa cggttacggt atctctgcgg atatcaagaa aatgaccaat atagaacata
     5041 tccatcaacg tagcgccgct tatggaattc ctggaatgtt catcgaagac ggtaacaatg
     5101 tcatcgatgt ctatgaagga tttcagaaag ctgtggacca tgttcgcagt ggcaatggtc
     5161 cagtcttgat tgaaagtgta acttatcgct ggctcggtca ctcatcatct gaccctggta
     5221 aatatcgtac gcgtgaagaa gtggaattgt ggaaacaaaa agatccaatc gaaaacctcc
     5281 gcaattacct tattgaaaat aacattgcaa gtgccgaaga attggaagaa atccaagcgc
     5341 aagtaaagga agcagtagaa gcttctgtta aatttgcaga ggaaagccca ttcccaccgc
     5401 ttgaatcagc ctttgaagat atttacgcag actaaggaga aagagataaa atggaaacaa
     5461 aaacaatgtc cttccgtgac accattatcc ttgctatgtc tgaggaaatg cgtcgcgatg
     5521 aaaatgtgtt cttgatggga gaagacgtcg gtgtcttcgg aggagacttc ggtacttctg
     5581 ttggaatgct tgaagaattt ggtccagaac gtgtccgtga ctgtccgatt tctgaagctg
     5641 ccatctctgg agcagcagca ggagcagcca tgacaggact tcgtccaatc gtcgatatga
     5701 ccttcatgga cttctctgtt attgccatgg acaatatcgt caaccaagct gctaaaacac
     5761 gttacatgtt tggtggtaaa ggtcaggttc caatgactgt tcgttgtgca gctggtaacg
     5821 gagttggctc tgccgcccag cactcacaat ctctagagtc ttggtttact cacattccag
     5881 gacttaaggt tgtggcacct ggaacacctg cggacatgaa aggcctgctt aagtcttcta
     5941 tccgtgataa caacccagtt attatacttg agtacaagtc agaatttaac caaaaagggg
     6001 aagtgccagt tgatccagac tacacaattc cacttggggt tggtgaaatc aaacgccaag
     6061 gaacggatgt aacagttgtt gcttatggaa aaatgcttcg ccgtgtggtt caagctgctg
     6121 aagaattagc agaagaagga atttcagttg aaattgttga cccacgtacc cttgttccgc
     6181 ttgataagga tatcatcata aactcagtga agaagactgg taaggttgtt ctggtcaacg
     6241 atgcccacaa aacaagtggc tatatcggtg aaatttcagc tattatttca gaatcagaag
     6301 catttgacta tctagatgca ccaattcgcc gttgtgcagg agaagatgtg ccgatgcctt
     6361 atgcacaaaa cctagaaaat gcaatgattc caacagttga aagtatcaaa gatgcaatcc
     6421 gaaaaactta taacaaagaa tagggttaca taagataagt tatgtaaata aaataaagct
     6481 gacgagaaac tttgtatctt ttaggtgcag agctcttcgt cattaatatc ttagattaga
     6541 gcacgggcta aaagatcgga aaaaagataa atctccttgg cttcatcgaa gccatcgtcg
     6601 atttcctatt tttcagtcgc ttttgacgcc ctttgtatca taaatagaat tggagaggtc
     6661 atggctgatg acaagctaag agcgactcct gcagctagaa agttagcgga tgatttaggg
     6721 ataaacctct acgacgtttc tggctcaggt gcaaacggtc gtgtccacaa agaagacgtg
     6781 gaaacttata aagacacaaa cgtggttcgc atttcgccac ttgcaaaacg aattgccctc
     6841 gaacataaca ttgcttggca ggaaatccaa ggaaccggtc atcgtggtaa aatcatgaag
     6901 aaggatgttt tggccctgct tcctgaaaat atcgaaaacg atagcatcaa atctcctgct
     6961 cagattgaaa aagtggaaga agtccctgat aacgtaacac catatggtaa aatcgagcgt
     7021 attccaatga caccaatgcg taaggttatt gcccaacgca tggttgaatc ttacttgact
     7081 gcgccaacct tcaccctcaa ctatgaagtt gatatgactg aaatgttggc tcttcgtaag
     7141 aaggttcttg agccaatcat ggaagcaact gggaagaaga ctactgtaac agaccttctt
     7201 tcgcttgcag ttgttaagac tcttatgaaa cacccataca tcaacgcttc attgacagaa
     7261 gatggcaaga ctattatcac tcacaactat gtcaatcttg ccatggcagt tgggatggat
     7321 aatggattga tgacacctgt tgtttacaat gctgagaaga tgagtctttc agaactggtt
     7381 gtagccttta aggatgttat tggccgtacc ttggatggta aattggctcc aagtgagtta
     7441 caaaattcaa cattcacaat cagtaatttg ggaatgtttg gtgttcagtc ctttggtccg
     7501 attattaacc aacccaactc agctatcctt ggtgtcagtt cgacaatcga gaagccagtt
     7561 gtcgtcaatg gtgaaattgt gattcgccca atcatgagtt taggattaac cattgaccac
     7621 cgtgtcgtag atggtatggc tggtgctaag tttatgaagg acttgaaaga gttgattgaa
     7681 acgccaatct caatgttaat ttaagagcaa gagtattcat ttttaagata tagataaagg
     7741 aaggaagaca tggccttaga agtaattatg ccaaaagccg gcgtggatat gacagaagga
     7801 caaatcgtcc aatggaataa aaaagtcgga gaatttgtaa aagaaggaga aatccttttg
     7861 gaaatcatga ctgataaagt cagcatggaa ttggaagccg aagaagatgg gtacttgatt
     7921 gccattctca aaggagatgg tgaaactgtc cctgtaacgg aagttatcgg ttaccttggt
     7981 gaagaaaggg aaaacatccc aacagctgga gcagcatcgc cagaagctag ccctgtacct
     8041 gtagcaagta cctcaaacga tgatggtaag agcgatgatg cttttgatat cgttgtgatt
     8101 ggtggaggtc ctgctggtta tgttgcagcc attaaagctg cccaattcgg cggtaaggtt
     8161 gcccttgttg agaaatctga acttggtgga acctgcttaa accgtggatg tattccaacc
     8221 aagacctacc ttcataacgc tgaaattatt gaaaatatcg gtcatgctgc aaatcgtggt
     8281 atcgtgattg aaaatcctaa cttcactgtt gatatggaaa aacttttaga aactaaatct
     8341 aaagttgtta atactcttgt tggtggtgtt gcaggacttc ttcgtagcta cggagttact
     8401 gttcataaag gaattggtac aatcactaaa gacaagaacg tcttggtaaa tggttctgaa
     8461 ttgcttgaaa ccaagaaaat cattcttgct ggtggttcaa aagtcaacaa gatcaacgtc
     8521 cctggtatgg aatctccact tgtcatgact agtgatgaca ttcttgaaat gaacgaagtg
     8581 ccagaaagcc ttgttatcat cggtggtgga gttgtcggta tcgaactcgg tcaggccttc
     8641 atgacatttg gttcaaaagt gactgttatc gaaatgatgg accgtatagt tccagctatg
     8701 gatgtggaag tttctaagaa ccttcgcttg attcttgagc gtaaaggaat gaccatcttg
     8761 actggtacta aactgcaaga aatcattgag gaaaatggtc aacttcgtat caaggttgaa
     8821 ggaaaagacg atatcatcgc aagcaaagct cttctttcaa ttggtcgtat gccagacctt
     8881 gaaggtattg gagaggttga gtttgaattg gatcgtggtt gtatcaaggt caacgaatac
     8941 atggaaactt cagttccagg catttatgca ccaggtgaca tcaacggtac taagatgttg
     9001 gctcacgcag ctttccgcat gggtgaagtt tccgctgaaa atgcccttaa aggaaatcat
     9061 gcagttgcca aattgaattt gactcctgca gccatctaca ctctccctga agtagcagca
     9121 gtaggtttga cagaagaaca agcccgtgag aaatacgatg ttgccatcgg taagtttaac
     9181 tttgctgcta acggtcgtgc tattgcatct gacgcagctc aaggtttcgt aaaagttatc
     9241 gctgataaga aatacggaga aatccttggt gtacacatta ttggtcctgc agccgcagaa
     9301 ttaataaacg aagcatcaag catcatcgaa atggaaatca ctgttgagga aatgctgaag
     9361 accatccacg ggcacccaac ttactctgaa gtgatgtacg aagcgtttgc agatgttcta
     9421 ggaatggcca tccattcacc taagaaaaaa taaaacgaag atagattaga ctggaaggtt
     9481 atttccagtt tctatcatat aaagtaaagg gatatcatga aatacattat caatcattca
     9541 aatgacactg cttttaatat tgccttggaa gaatacgcct ttaaacacct tttggatgag
     9601 gatcaaatct tcctactttg gattaacaag ccatctatca ttgttggtcg tcaccagaac
     9661 actatcgaag aaatcaaccg tgattatgtt cgcgaaaatg gtattgaggt agttcgccgt
     9721 attagtggtg gtggagctgt ttaccacgat ttaaataacc tcaactacac gatcatctcc
     9781 aaagaagatg aaaataaggc atttgacttc aagagcttct caactccggt tatcaatacc
     9841 ctggctcaac ttggggttaa agctgagttc acaggtcgta atgaccttga gattgatggt
     9901 aagaagttct gtggcaatgc ccaagcctat atcaacggcc gtatcatgca ccacggttgc
     9961 ttgctctttg acgttgattt gtcagtcctc gcaaatgccc ttaaggtttc aaaagataaa
    10021 tttgaatcaa aaggtgtgaa atccgtccgt gcccgtgtaa ccaatattat caatgaatta
    10081 ccaaaaaaaa tcacagtcga aaaattccgt gatttactct tggaatacat gaaaaaagag
    10141 tacccagaga tgactgaata cgtcttttca gaagaagaat tggccgaaat caatcgcatc
    10201 aaggatacta agtttggaac ttgggactgg aactatggta aatcacctga atttaacgtc
    10261 cgtcgtggaa tcaaattcac tagtggtaag gttgaagtct ttgctaacgt tactgaatca
    10321 aaaatccaag acatcaagat ttatggtgac ttctttggta ttgaagacgt tgctgcagta
    10381 gaagatgtcc ttcgtggggt gaaatacgaa cgcgaagatg ttcttaaggc gctaaaaacc
    10441 attgatatca cacgctactt cgctggtatt agccgtgaag aaatcgctga agcggtagtt
    10501 ggataaatca aaaagaagtt ggccaacttc tttttttgat atttatttac ataatttaaa
    10561 atacgtaaaa tcataaacta tccagagcat tcttttgttc atcactaaca atatgggtat
    10621 agaggtcagt gacttgtgtg ctagcatgtc ctagttggtg actgactaaa acttgtgatt
    10681 tagtcgcatc atagagccta gttgctagtg tatggcgcag tttatggggt gttacacgca
    10741 ctttaaaatc ctctgagtat ttagcaacca ttttctcaac gctagaagca tcgatacgat
    10801 taggaacacc tctgtagaga gttaaaaaaa gggctgtatc tgttttttcc gttttatagc
    10861 gttgattccg aatggccaga taattctcta aataaggttt agcaaaagca gcgacattga
    10921 ctgagtcacg tttgcaacct tttcgagtaa catcaataac catcattttt agattgagat
    10981 ctcttagatc tagattaaca gcttcagata agcgaacacc agatgccaag agaagggcaa
    11041 taatggctaa atctcgttct ttatttttgt tgaatgatga gagagctcga tttgaaagct
    11101 gttgtgggtg ctcttgatcg atataagtta gaaaaccttc tgtttcatca cctagaaaga
    11161 gtttttgctt gatattttca gctctggcag caagggtttc tttctttttc ttggtggaaa
    11221 cttttttcat tacattacga tagaaataag gttccccctg atcgttttca acctcctcgg
    11281 ttagatactt gtaaagacta gaaagtgctg ataaggttcg attgatagtt gtctgtgaaa
    11341 caccttgttt tgttgtatta gcattcagca agggacgttc acgtagataa aggataaagg
    11401 attccatgtc tttcttagac atattttcca aaactgataa aggaatatcg gatattttat
    11461 cagcgtttga aataccagac tccaaaaccc agctgaaaaa tcggtcatat tccttaaggt
    11521 attcgtacag ggttgtaaaa ctgtagggca cagccagctt agattggtag tattccagaa
    11581 cataccaggg catgagttgt tttagtttgt cgattcgttc cagtaaaatc tcacgtttca
    11641 tgtattttct ccataagtaa gtatactagt agtatagcat agacttgtat atttttcaag
    11701 aaaattatag tttttcggaa agatgttata ggagtttttc aaagtgataa aaagacttag
    11761 acctacaaac aaaacagaat aaggaaaata ataaaaatct cacccactaa acttctgtta
    11821 atactacata actgaagagc tatataagga gctatgagat acaaaacaca ttgtataata
    11881 gattgaaact agaatagtac atctggactt ctaaaacatt gctagaaatt gatttgattg
    11941 tcctgattgt tttatcctat ttctatttca tttctctata taattgctat tgttttcttt
    12001 ttc
//
LOCUS       DAVHEW010000060        11993 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVHEW010000060 DAVHEW010000000
VERSION     DAVHEW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.060"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(95..1468)
                     /gene="mnmE"
                     /locus_tag="VVL53_001459"
     CDS             complement(95..1468)
                     /gene="mnmE"
                     /locus_tag="VVL53_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU4183618.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQETNRII
                     LLNKTDLPETIETSELPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(1605..1787)
                     /locus_tag="VVL53_001460"
     CDS             complement(1605..1787)
                     /locus_tag="VVL53_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU4183619.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            1911..2510
                     /locus_tag="VVL53_001461"
     CDS             1911..2510
                     /locus_tag="VVL53_001461"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU4183620.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            2500..2979
                     /locus_tag="VVL53_001462"
     CDS             2500..2979
                     /locus_tag="VVL53_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183621.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            2989..4068
                     /gene="prfA"
                     /locus_tag="VVL53_001463"
     CDS             2989..4068
                     /gene="prfA"
                     /locus_tag="VVL53_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU4183622.1"
                     /translation="MNIYDQLQVVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            4068..4907
                     /gene="prmC"
                     /locus_tag="VVL53_001464"
     CDS             4068..4907
                     /gene="prmC"
                     /locus_tag="VVL53_001464"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU4183623.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILAENPVKNLMVLDIGTGSGAIALALAKNRPDWSVTAADISQDALDVASENAKNQNLQ
                     IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            4891..5493
                     /locus_tag="VVL53_001465"
     CDS             4891..5493
                     /locus_tag="VVL53_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU4183624.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     RRDKALNLNVASLEDILYFSKNQPTYLQKLVEIFLPGPLTIILEANDRVPYWVNSGLA
                     TVGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFNQILEDFDQEVLGLEDDAFL
                     TGQDSTILNLSGDKVKILRQGAIKREDILARLPKISFEEE"
     gene            5495..5926
                     /locus_tag="VVL53_001466"
     CDS             5495..5926
                     /locus_tag="VVL53_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183625.1"
                     /translation="MLRDLRETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            5988..7244
                     /locus_tag="VVL53_001467"
     CDS             5988..7244
                     /locus_tag="VVL53_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU4183626.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            7252..8226
                     /locus_tag="VVL53_001468"
     CDS             7252..8226
                     /locus_tag="VVL53_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU4183627.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAKSFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            8226..8840
                     /gene="pmp23"
                     /locus_tag="VVL53_001469"
     CDS             8226..8840
                     /gene="pmp23"
                     /locus_tag="VVL53_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU4183628.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            8914..9888
                     /locus_tag="VVL53_001470"
     CDS             8914..9888
                     /locus_tag="VVL53_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU4183629.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKEFKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDSDKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            9962..11593
                     /gene="rlmD"
                     /locus_tag="VVL53_001471"
     CDS             9962..11593
                     /gene="rlmD"
                     /locus_tag="VVL53_001471"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU4183630.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLIRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVYKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            11747..11896
                     /locus_tag="VVL53_001472"
                     /pseudo
     CDS             11747..11896
                     /locus_tag="VVL53_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT         3747 a         2292 c         2558 g         3396 t
ORIGIN      
        1 taaaataggc tccataatat ctatagtgga tttacccact acaaatatta tagaacctaa
       61 aatccatgac cgaatgaagg atcatggatt tttcttattt tcctaaacag aattggctaa
      121 agagttgggt gatgagttca tctggagcag catctccagt gatttctcct agaatttccc
      181 aagtacgggt caagtcaact tgaagcaagt caactggcat ccctagttca agaccttggt
      241 taacagcttg taggctttca acggccttct caatcaagga aatgtgacgg gcgtttgaca
      301 agtaggtagc atcttgctca accaaaccag cattttcaaa gaagaggttg ttgattctct
      361 cttcgatttt atcgatgttt tgatttttaa gaactgaaat gcggatgaca tcttcaggta
      421 gttccgaagt ttcaatcgtt tcaggcaggt cagttttgtt aagaagaata atgcgattag
      481 tctcctgact gatttctagg agttggcgat cttgggcggt tagtggttca ctagcgttta
      541 gtactagcag aactaggtca gcttccttaa gagctttttt cgaacgctca actccaattt
      601 gttcaacgat atcatccgtt tcacgaatac cggctgtatc aatcaatttg agaggtacac
      661 cattgatgtt gacgtactct tcgatgacat ctcgtgttgt cccagcgata tctgttacga
      721 tagccttgtc ctcacgcaag aggttgttga gaaggcttga tttcccaacg ttgggacgtc
      781 caatgatagc cgttgaaatt ccttcacgaa ggattttacc acgacgtgct gtcctaagga
      841 gtttggttag taattgctca aactccattg tcttctctcg gacaacagca gtagtggctt
      901 cctcaacatc gtcatactca ggatagtcga tattgacctc aacttgggca agtgtattga
      961 ggatttcttg acgggtattg ttaatgaggt cagaaaggga gccgtctaat tgtttgaccg
     1021 caatgttcat ggccttgtca gtcttggcac ggatgatatc catcacagcc tctgcctgtg
     1081 tcaagtctac gcgaccgttt aaaaaagcac gtttggtaaa ttcaccaggt tctgccaacc
     1141 gagccccttc acgaatagct agctggagaa tttcattggt caccgcaatc ccaccgtggg
     1201 tgttaatctc gataatatcc tcacgagtga aggtctttgg agacttcata gccccaacca
     1261 taacctcgtc catgacttta ccagtcagag gatcaataat gtgaccgtag ttgagagtgt
     1321 ggctggcaac cttgttcaag tcttttcctt taaaaatctt ttgcgcaata gcaaaactgt
     1381 ctgttccgct caggcggaca ataccaatag ccccttcacc tagtggagta gagatagcag
     1441 cgatggtatc aaattcacgt gtaatcataa tgtttccttt aattaatagc tcttttcttg
     1501 gaaattctcc aagtttcttt tcaaattgta acaaatttag gatatttctt caatgaatgc
     1561 tacttggaga ttgaaaaagc ctaagcaatt ctgcttaagc tagcttattt agtacgcatt
     1621 tccccttgtg ggaagtaagt tccttctggc atgtcgttga tgatgacatg gacagcagat
     1681 tgaggggctc cagtgttgcg gacaactgct tccgttactt ccttagcaag agctttcttt
     1741 tgctcgagcg tgcgtccttc aaataaatcg atgcgtacaa atggcataat agcttcctcc
     1801 actagttttt gatttcttct attttaccac attttgccgt ttaaagctta agaaaattat
     1861 gatatactag aatgtagcaa aaatttagaa atggacgtga agcaagaaac atggcacagt
     1921 tgtactatcg ttatgggacc atgaactctg gtaaaacgat tgagattctc aaagtggcct
     1981 ataactacga ggagcaagga aaaggtgttg tgattatgac ctcggctctg gatacgcgtg
     2041 acggcgttgg ctatgtgtcg agtcgaattg gcatgaaacg ccctgccctt gcgattgagg
     2101 aaacaacgga tatctttggc tatatccgag acctaccaga aaaaccttac tgtgttttgg
     2161 tcgatgaagc ccagtttctc aaacgccacc atgtttacga cctagctcgt gttgtggacg
     2221 agttagacat acccgtcatg gcttttggct tgaaaaatga ctttcgcaat gaattgttcg
     2281 aaggttccaa atatctcttg cttttagcag acaagattga cgaaatcaag accatctgtc
     2341 agtattgcaa gaaaaaggcg accatggtgt tgcgaacaca ggatggactg cccgtttacg
     2401 atggagaaca gatccagatt ggtggtaacg aaacctatat ctcagtttgc cgtaaacatt
     2461 attttgcgcc aatgataacc tctaacaagg agcaaaacta tgacaattga actaagagat
     2521 gttacaatgg aaaattattt tgatgtttta aatttggatg tcaaggaata tcaaaaacaa
     2581 ttcattgcaa ccaacgcaat tagtttagct gaagcatacg tctacactaa aaatggagat
     2641 tttgtagctc cattggcagt ttatgataat gatgcaatta taggttttgt gatgatagct
     2701 tatgataaaa agatcggaat tagtagtgga aattatttac tatttcgttt tatgattgat
     2761 aagaattttc aaaatcaagg atattttaaa ccaattatgg ataaagtact agactatgtt
     2821 cgaacagcgc cagcaggttt aagcaataaa ctttggttgt cttatgaacc agaaaatgaa
     2881 caggcaagat tttgttacct cagttatgga tttaaagaaa ctggggaaat atccgagaat
     2941 gaagtagtag caatctatga tttaacaatt gagaaataag gagaaaaaat gaacatctat
     3001 gatcaactac aagttgtaga agaccgttat gaagaattag gagaattgct gagtgaccct
     3061 gatgtcgttt cagacaccaa gcgttttatg gagctttcaa aagaagaagc ttccaatcgt
     3121 gacaccgtaa tagcctaccg tgagtataaa caagtccttc aaaatatcgt cgatgccgaa
     3181 gagatgatta aggaatcagg cggagatgcg gacttggaag aattggccaa gcaagaactc
     3241 aaagatgcca aggctgaaaa agaagaatat gaagaaaaac tgaaaatttt gctccttcca
     3301 aaggatccaa acgatgacaa gaatatcatc cttgaaatcc gtggagcagc tggtggagac
     3361 gaagcggcac ttttcgctgg agatttgcta actatgtacc aaaagtatgc ggaagcccaa
     3421 ggttggcgct ttgaagtcat ggaagcctct atgaatggtg tcggtggttt taaagaagtg
     3481 gttgctatgg tttcaggtca gtctgtatac tctaagctta agtatgaatc aggtgcccac
     3541 cgtgtgcaac gtgttcctgt gacagaaagc caaggccgtg ttcatacttc gacagcgaca
     3601 gttcttgtta tgccagaagt tgaagaggtt gaatacgaca ttgatccaaa agaccttcgt
     3661 gtcgacatct atcacgcctc tggtgctggt ggacagaacg tcaataaggt tgcgactgcc
     3721 gttcgtatcg ttcacttgcc aaccaatatc aaggttgaga tgcaggaaga acgtacccag
     3781 cagaagaacc gtgagaaggc catgaaaatc atccgtgccc gtgttgctga ccactttgct
     3841 cagattgctc aagatgagca ggacgctgag cgtaagtcga caatcggtac tggtgaccgt
     3901 tcagaacgga tccgaactta taacttccca caaaaccgtg tcacagacca ccgtatcggc
     3961 ttgaccctcc aaaaactaga tacgattttg tctggtaaat tggacgaagt tgtggatgcc
     4021 ttggtgcttt atgaccaaac acaaaaacta gaagaattaa acaaataatg aaattagctc
     4081 aattattttc aaattttgaa gaagagttga taagacaagg agaggaagcg gaaagcctct
     4141 cttttgtcta tcgtagtctg aaaaatctat cttttacaga ctttattttt gccctccagc
     4201 aggaggtgac aacagaggaa gaaaaacaat ttgtagaaga tatttaccag caactagcag
     4261 ctcacaagcc agctcagtac atcattggac aggcagattt ttatggaatg catttaaagg
     4321 tggatgaacg ggttttgatt cctcgtccag aaacagagga gttggtggag cttatcctgg
     4381 ctgaaaatcc tgtgaaaaat cttatggtcc tagatatcgg aacaggtagt ggagctattg
     4441 ctctcgctct agcaaaaaac agaccagatt ggtcagtgac ggcagcagat atttcccaag
     4501 atgctttaga tgtagctagc gaaaatgcta aaaatcaaaa tcttcaaata tttttaaaaa
     4561 aatctgactg ttttacagaa atttctgaaa aatatgatat aattgtttcc aatccaccct
     4621 atatctctcg tgaagatgag tcagaggtag gcttgaatgt cttgtattcg gagcctcatc
     4681 tagctctctt tgcagatgag gatggcctag ctatttaccg tagaattgcg gaagatgcaa
     4741 aagactatct caaagatggt ggtaagattt accttgaaat tggatacaag caaggtcaaa
     4801 gtgttcctga actttttagg aaacatcttc ctgaaaagcg agtacgaaca ctcaaggacc
     4861 aatttggtca agataggatg gttgtcgttg atgatggaca ggattagaca agagttggaa
     4921 aatggtggag ctgtcgtttt gcctacagag acagtttacg gtctctttgc taaggcgcta
     4981 gacgaaaaag cagttgacca tgtttaccag cttaaacgtc gacgtagaga caaggcgctc
     5041 aatctcaatg ttgcctcttt agaggacatc ttgtactttt caaagaatca gccaacttat
     5101 cttcaaaaac ttgtagagat ctttttacca ggtcccttga ccattattct cgaagctaat
     5161 gaccgagttc cctattgggt caattctggc cttgcaactg ttggatttcg gatgcctagt
     5221 catcccatta cccttgattt gattcgagag acaggtccct tgattgggcc gtctgccaat
     5281 atctcaggtc aggcgagtgg agtgaccttt aatcaaattc tagaggattt tgaccaagag
     5341 gttctgggtc tggaagacga tgcttttcta actggacagg attcaactat tttgaatttg
     5401 tcgggagaca aggtgaaaat cttacgtcaa ggggccatta agcgagaaga tattcttgct
     5461 cggttgccaa agatttcttt tgaggaggaa tgagatgcta agagatttaa gagaaactga
     5521 tgtgaaagct atatgtgaca tcaaccaaga ggctttgggt tatactttta gtccagagga
     5581 aacggctagc caactagcta gactgtctca ggattcccat catttcctac ttggctatga
     5641 ggatgcagct aatcatgtct tacttggata tgtccacgct gaagtttacg aatcactcta
     5701 ttccaaagca ggatttaata tcttagcttt agcagtttca cctcaagcgc aaggtcaagg
     5761 tattggtaaa agtttactac aagggttgga agaagaagca aaaagatgtg gttatgggtt
     5821 tatccgctta aattctgcca atcatcgtct gggtgctcat gcattttatg aaaaagttgg
     5881 ctatacttgt gataaaatgc agaaacggtt tattcgcatc tgttagtttg attttcttat
     5941 tgtaaaatca aactaatgga ctagtcacac aataaaggag aagacctatg atttttgaca
     6001 aagatgattt taaagcatat gatgctgatc tctggaatgc tattgccaaa gaagaagaac
     6061 gccaacaaaa taatatcgag ttaattgctt cggaaaacgt agtttccaag gctgttatgg
     6121 cagctcaagg gtctatcttg acaaataaat atgccgaggg ttacccagga cgccgttatt
     6181 atggtggaac tgatgtagta gacgttgtag agactcttgc tattgaacgc gcaaaagaaa
     6241 ttttcggtgc taaatttgcc aatgttcaac cacattcagg aagccaagct aactgtgcgg
     6301 cttacatgtc cttgattgag ccaggtgata cggttatggg aatggatttg gcatcaggtg
     6361 gtcatttgac tcatggggct cctgttagct tctctggtca aacctacaac tttgtttctt
     6421 atagtgttga tcctaaaacg gaactcttag actttgatgc tatcttgaaa caagcccaag
     6481 aagtaaaacc aaaactgatt gtagctggtg cttcagccta ttctcaaatt atcgattttt
     6541 caaaattccg tgaaatcgca gatgctgtcg gtgcgaagct catggtggac atggcccata
     6601 tcgctggctt ggttgcggct ggccttcatc caagcccagt tccatacgct catatcacaa
     6661 caacaacgac ccacaaaacc cttcgtggac ctcgtggtgg tttgattttg accaatgacg
     6721 aagaacttgc taaaaaaatc aattcagcta ttttcccagg tattcagggc ggtcctttag
     6781 agcatgttgt ggcggctaag gcagtttcct tcaaagaagt tttggatcca gccttcaagg
     6841 aatatgctgc caatgtaatt aagaacagca aggctatggc agatgtcttc ttgcaagacc
     6901 ctgatttccg tattatttca ggtggaactg aaaaccatct cttccttgtt gatgtgacta
     6961 aagttgtaga aaacggcaaa gttgctcaaa acttgttgga tgaagtcaat attaccttaa
     7021 ataaaaactc aatcccttac gaaagcttgt caccattcaa gacaagtggg attcgtatcg
     7081 gagcagcagc cattactgca cgtggatttg gtgaagaaga aagtcgcaaa gtggctgaac
     7141 tcatcattaa aacccttaag aattcagaaa atgaggctgt attagaagaa gtgagaagtg
     7201 cagtcaaaga attgacagat gccttcccat tatacgagga ctaaaacttt tatggacatt
     7261 tatattaaga aagccattat tcaccagttc agtccggatg ataccgaact gttcttagca
     7321 gataagtttc tcaatattac tccaaaaatc gaagaatacc tgcgtaaaaa aattgaacat
     7381 gtgtattcag atgaagccaa gactgggatt ttcgaagaag aaaatccctt cttcaatcat
     7441 attacagacg atttgttgga gacatcagta acgctggcta atctctggaa agaggagttt
     7501 agcatttctg aaaatctcaa gaccaatgac ttgatttttg tgcaattttc taaagaaggt
     7561 gtagaacatt tcgctttctt gcgaattgcc ctgcgggaga ccttgaccca cctcggagga
     7621 gaagttgata atccaatcaa gctaactcag aataacctgc ctggatttgg aacaggagct
     7681 gatgaggcct tggtggtcaa tcttcagagt agcaagtatc acctgattga aaaacgaatc
     7741 aagtacaacg ggactttttt gaactatttt tcagataatc ttcttgctgt cgctcctaag
     7801 atttctccta aaaaatctat caaggaactg gaaaaaacag cccagagaat tgctaaatct
     7861 tttaacacag atgattttca atttcaatcc aaggtcaaat cagctatttt caacaaccta
     7921 gaagaaagca atgaattgtc acctgagaaa ttggctaatg acctttttga caacaatctg
     7981 acggctcgtt tgagctttat tgaccaagtc aaagaagccg taccagaacc tgttcaattt
     8041 gatgaaattg atgccagtcg ccaattaaag aaatttgaaa accaaaaact ctccttatca
     8101 aatggaattg agctcatcgt tcccaataac gtctatcaag acgccgagtc tgttgagttt
     8161 atccaaaacg aaaatggaac ctactctatc ttaatcaaaa atatcgagga tatccaaagt
     8221 aaataatgtt taaacgaatt cgaagagtgc ttgtactagc agtcttcctt tttgctggct
     8281 ataaagctta ccgcgttcat caagatgtca aacaagtcat gacctatcaa cccatggtgc
     8341 gagaaatctt gagtgaaaaa gacaccccag caaacgaaga gcttgtgctt gctatgattt
     8401 atactgaaac aaaaggaaaa gaaggcgatg ttatgcagtc tagtgagtct gcaagtggtt
     8461 ccaccaacac catcaatgat aatgcctcta gcattcggca aggcattcaa actctgacag
     8521 gcaatctcta tctggcgcag aagaagggtg tagatatctg gacagctgtt caagcctata
     8581 attttggacc tgcctatatc gattttatcg cccaaaatgg caaggaaaat accttggctc
     8641 tagccaaaca gtactctcgt gagactgttg cccccttgct tggtaatacg actggaaaga
     8701 cttatagtta tattcacccc atttccattt ttcacggtgc tgaactctat gtaaatggag
     8761 gaaactatta ttattctaga caggtacgac ttaaccttta cataatcaaa tgtttcactc
     8821 tcttttcaac atctggctag accaggtgtt tttgttataa gttttcttta agatagatat
     8881 attactataa agaagtaaag gaggaaattc cctatgagaa agaaactctt tctgactagt
     8941 gctgcggtct tgtgggcagt aacagctatg aatagcgtcc atgcagcaac agatgttcaa
     9001 aaagttatcg atgaaaccta tgtccaacct gaatatgtcc taggttcctc cctatctgaa
     9061 gaccaaaaaa atcaaactct taaaaaactg ggctacaatg cctcaacaga taccaaagaa
     9121 ttcaagacca tgacacctga tgtttattct aaaatcatga atgtggccaa tgactctagc
     9181 ttacagttgt attcatcagc caagattcaa aagctaggtg acaaatcgcc acttgaggtc
     9241 aagattgaaa caccagaaaa tatcactaag gtgactcagg atatgtaccg aaacgcagca
     9301 gtaacgctgg gtatggaaca tgccaaaatc actgtagcag cccctattcc agttacaggt
     9361 gagagtgctt tggctgggat ctactattcg ctagaagcta atggagccaa ggtgccgcaa
     9421 gccaataaag atttggctca agaagagcta aaggctttgt cagatatcaa tgctgaaaat
     9481 aaggacaaat caggctatga tgctaataaa ttaaacgttg ccctagctga tatcaagtca
     9541 ggactcgcca aagctaaaga aagcaaggga aatctgacag aagaagatgt acgcaagatt
     9601 gttgaagata ccttaaaaaa ttacaaactt gatcaggtca taacaggaaa ccagatcaat
     9661 atcatcatca attttgcctt gaatctctca aagagtgata tcctcagcaa tgcagatttc
     9721 actaaaacct taaatgacct taaacaaagc atcgtatcac aagctggcga cagttttaaa
     9781 aatatcaacc ttaactttga ttcggataag gcgctagagg acggtggtaa cttcttaagc
     9841 tccctctggc aagcccttgt caacttcttc aagagttttg gttcttaaga aatttcatgg
     9901 tataatggat ggtaaccgta acgttgccgt cttttttgtc ggccaataga aagagaaaag
     9961 aatgttaaag aaaaatgata ttgtagaagt tgaaattgtt gatttgaccc atgaaggggc
    10021 aggagttgcc aaggtagatg gtttggtctt ttttgtagag aatgctttac cgagtgaaaa
    10081 aattctcatg cgtgtcctca aggtcaataa aaagattggc tttggaaaag ttgaaaaata
    10141 ccttgtccag tcaccacacc gtaatcaaga tctagatttg gcttacctgc gttcaggaat
    10201 cgcggattta ggacaccttt cttatccaga acagctcaag tttaaaacca agcaagtcaa
    10261 ggacagtctc tacaagattg ctggaattgc agatgtagaa gttgctgaaa cgcttggtat
    10321 ggaacatcca gtcaagtatc gcaataaggc gcaggtgccc gttcgtcgag tgaatggtgt
    10381 cttggaaaca ggatttttcc gtaagaattc gcacaacctc atgccccttg aagatttctt
    10441 tatccaggat cctgtcattg accaagtcgt agtagctctt cgagacctga tccgtcgttt
    10501 tgatttaaaa ccttatgacg aaaaggaaca gtctggatta attcggaatc ttgtggtgcg
    10561 tcgtggccac tattcaggac aaatcatggt cgttttggtg acaactcgtc caaaagtttt
    10621 tcgtgttgac caattgattg aacaagttat caagcagttc ccagagattg tgtctgtcat
    10681 gcaaaatatc aacgaccaga ataccaatgc gatttttggt aaggagtggc gcactcttta
    10741 tggtcaagac tatattacgg accagatgtt gggaaatgac ttccaaatcg ctggcccagc
    10801 cttttaccaa gtcaatactg aaatggcgga gaaactctat caaacagcca ttgactttgc
    10861 agagttaaaa aaagatgacg tgattattga tgcctattct ggtattggaa ccattggttt
    10921 atcagtcgcc aagcatgtca aagaagtcta cggtgttgaa ctgattccag aagcggttga
    10981 gaatagtaaa aaaaatgctc agctgaacaa tatttcaaac gcccactatg tctgtgacac
    11041 agctgaaaat gctatgaaga attggcttaa agatgggatt caaccaaccg ttatcttggt
    11101 tgatcctcca cgcaagggct tgacagaaag ctttatcaaa gcaagcgccc aaacaggagc
    11161 cgatcgcatc gcctatatct cctgcaatgt cgcaaccatg gcgcgtgata ttaaactata
    11221 ccaagagttg ggatatgaat tgaagaaagt ccagccggtg gatctatttc ctcaaacgca
    11281 tcacgtcgag acggtagcac ttttgtccaa actcgatgtc tataagcaca taagtgttga
    11341 aattgagctg gatgagatgg atttgacaag tgcggagagc aaagcaacat atgctcaaat
    11401 caaagaatat gtttggaata aatttgaatt aaaagtttcg acattatata ttgcacagat
    11461 aaaaaagaaa tgtggaatag aattacgaga acattacaac aagtctaaaa aggataaaca
    11521 aattattcca cagtgtacac ctgaaaaaga agaagccatc atggatgctt tgagacactt
    11581 caaaatgatt taatagaaaa gaatgacagt atatgacttt ctgcatttat tacattccta
    11641 cttggtatag gaacagctat tattcctttc ttgcaaggta tcaattagaa aataggctca
    11701 atataaagat tgataggatc atttttatat ttaaaggagc gttgaaatga ttgataaagg
    11761 caacaaaaaa ttttaggata aatttgctaa gttgtatgcc ccttttatga aaaaagataa
    11821 agaggtttat gataaagttt gtgaatatct tagtcctcat ttgaataaag atatggaggt
    11881 gcttgaactt gcttgttggt ttcgtgtcat aacagttata gaggcaaata gttatgtaaa
    11941 tataaggagt tcaagacttc taccaaagtt taaaactcaa aaaataaata gtt
//
LOCUS       DAVHEW010000061        11746 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVHEW010000061 DAVHEW010000000
VERSION     DAVHEW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.061"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(341..1537)
                     /gene="tuf"
                     /locus_tag="VVL53_001473"
     CDS             complement(341..1537)
                     /gene="tuf"
                     /locus_tag="VVL53_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU4183631.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2459..3328)
                     /locus_tag="VVL53_001474"
     CDS             complement(2459..3328)
                     /locus_tag="VVL53_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU4183632.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKATEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3552..4160)
                     /locus_tag="VVL53_001475"
     CDS             complement(3552..4160)
                     /locus_tag="VVL53_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4183633.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(4118..5224)
                     /locus_tag="VVL53_001476"
     CDS             complement(4118..5224)
                     /locus_tag="VVL53_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU4183634.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5335..5589)
                     /locus_tag="VVL53_001477"
     CDS             complement(5335..5589)
                     /locus_tag="VVL53_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183635.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5947..6393)
                     /locus_tag="VVL53_001478"
     CDS             complement(5947..6393)
                     /locus_tag="VVL53_001478"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4183636.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6466..6791)
                     /locus_tag="VVL53_001479"
                     /pseudo
     CDS             complement(6466..6791)
                     /locus_tag="VVL53_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7025..8743
                     /locus_tag="VVL53_001480"
     CDS             7025..8743
                     /locus_tag="VVL53_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU4183637.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8854..9201
                     /locus_tag="VVL53_001481"
     CDS             8854..9201
                     /locus_tag="VVL53_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4183638.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9480..10316)
                     /locus_tag="VVL53_001482"
     CDS             complement(9480..10316)
                     /locus_tag="VVL53_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4183639.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10329..10958)
                     /locus_tag="VVL53_001483"
     CDS             complement(10329..10958)
                     /locus_tag="VVL53_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4183640.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10968..11609)
                     /locus_tag="VVL53_001484"
     CDS             complement(10968..11609)
                     /locus_tag="VVL53_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4183641.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
BASE COUNT         3440 a         2457 c         2143 g         3706 t
ORIGIN      
        1 ctcttcgaaa atctcttcaa accgcgtcag cttcgccttg tcgtatagat gttactgact
       61 tcgtcagttc tatctacaac ctaaaaacag tgttttgagc agtctgtagc taactttcta
      121 tagtaaaatg aaataagaac aagacaaatc gatcaggaca gtcaaatcga tttctaacaa
      181 tgttttagaa gtagatgtgt actattctag tttcaatata ttatagtttg ctctttgatt
      241 ttcattgagt attagttcaa tatctaaaaa aagaaccttg ccaagcaaga ttcttttagt
      301 gtctaactta aataattgtt cttctgggaa ctaaatcgaa ttaagcttcg atttctgtaa
      361 ccatacctga accaacagta cgtccaccct cacggataga gaatgtagta ccttgttcta
      421 cggcgattgg gtgaatcaac tcaacgtcga ttgtcacgtt atcaccaggc attaccattt
      481 cagtacctgc tggaagttcg attgaacctg taacgtcagt agtacggaag tagaattgtg
      541 gacggtagtt gttgaagaat ggagtgtgac gtccaccttc ttctttagta aggatgtaga
      601 cttcaccttt gaatttagtg tgtgggttga ttgaacctgg tttagcgata acttgtccac
      661 gttcgatttc atcacgttga acaccacgaa gaaggacacc tacgttatct ccagcaagac
      721 cttcgtcaag ttgtttacgg aacatttcaa caccagtaac aactgctttt tgagtttctt
      781 ctttgatacc aacgatttcg atttcgtcgt tgactttaac gataccacgg tcgatacgtc
      841 ctgaagcaac tgtaccacgt ccagtgattg agaatacgtc ctcgactgga agaagcaatg
      901 gtttgtcagt gtcacgttct ggttctggga tatactcatc aactgtgttc atcaattcca
      961 taacgatgtc ttcgtattta gagtcacctt caagagcttt aagtgctgaa ccttggataa
     1021 ctgggagatc gtcacctggg aagtcgtatt ctgacaatag gtcacggatt tccatttcaa
     1081 ccaattcaag caattcttcg tcgtcaacca agtcaacttt gttcatgaag acgataaggt
     1141 gtttaacacc aacctgacgt gaaagaagga tgtgctcacg agtttgtggc attggtccgt
     1201 cagttgaagc tactacaagg atagctccgt ccatttgagc agcaccagtg atcatgtttt
     1261 taacgtagtc cgcgtgtcct ggagcgtcga tgtgagcgta gtgacgtttt tcagtttcgt
     1321 actcaacgtg cgcagtgttg atagtgatac cgcgttcgcg ttcttctgga gcagcatcga
     1381 tagacgcata gtctttaggt tggttaactg atgaaggcaa gcgacgtgcc aaaacagttg
     1441 tgatagctgc agttagggta gttttaccgt ggtcaacgtg tccgatagta ccaatgttaa
     1501 cgtgtggttt actacgatcg tatttttctt ttgccatttg agtaaaagcc tccaataaaa
     1561 tatattttat agatagacag taggcaatac agtctaactt tccttactat tttatcaaat
     1621 ttcagctaaa ttgcaagtgt tttacaacgt ttttgtgaat tattcttggt aaaaatttaa
     1681 ctaattaata gaaagtacta aatagactta tccttcaagg agttttcagc attatccatc
     1741 cactcacttg ctgaaaatgt aaacagtctc tggtttatat agtaaaatga aacaagaata
     1801 gggcaaatcg ttcaggacag tcaaatcgat ttctaacaat gttttagaag tagatgtgta
     1861 ctattctagt ttcaatctac tatacaacct caaaactgtg ttttgaacaa cctgcggcta
     1921 gcttcttagt ttgccctttg attttcattg agtataaaat tgcagataaa acgataacaa
     1981 tcataggact cagcagagtc aatgtaggtc gaagaatagc ccccctaatc gcttatttta
     2041 tagtaaaatg aaatataaac tgaacatttt gttgaggaag gtaaatcaat tcctcacaat
     2101 gtttgagaaa gaagggagga ctattccaac ttcaacccac tataaagcag acttacaaga
     2161 tagggaaccc atctccgata atattccgat aatatatagt agaatgaaaa ctgcacaatt
     2221 tgattggaga attcaaacca atttctcaca atgttttaga aacaagggtc tattatttca
     2281 actgcaacct actatagtcc attgaagctg gaatagccca ctgagacttc taaaccactg
     2341 ctggagagga gtttgtttag agtttctttc atttccctaa aaaatcaaaa agagagaacc
     2401 agtctgatcc tctcttactt atagatggtg ctcactcaaa tgttaaagga gctatttctt
     2461 agagataaag gaatttgaat acagctactg ccgcaattgc tgctgcgata ggtgctacta
     2521 ctggtaccca agaataccac cattttgaat cgcctttatg ctcaccaaga actgatttgg
     2581 gaaggaaagc atgaaggaga cgtggtccca agtcacgggc tgggttcaag gcaggtcctg
     2641 taggtcctcc aagtgatgtt accaaagcca taacgaggaa tccaagtgcc aagtgagcca
     2701 cagaaagtcc tgaagcagtg tgtggagcca cctgtgcttt aatagccaag tcagaaaaat
     2761 caactgtttg tcctgcttct gttgcctttt gtttcatgaa ttgaagcact tcagcaccaa
     2821 agaagttttt agtcaaacca agagctgcaa agaacaaaac aaatgaacca acaaactcat
     2881 taatcaaacc attgacagtt gctgcatagc gactttcttt tgtaccatgg tcaatacttg
     2941 aaatagttga gaaagttccc aagatgttat ttgggttttc agttttcaag tagaatggac
     3001 ggtatgttgc cacaactaag gcttggccaa agatagcccc caagacttgc gcgataatgt
     3061 aaggtaccac ttgtgcccaa gggaaaagac cgctaactgc aagccctaga gtgaaagcag
     3121 ggttgatgtg attcccagat acgttaccaa acatcaaggc tgggatcata acccccatac
     3181 cataaccaac agcaatgacg atccagccac tttggtgacc tttcgtacct ttaagttcaa
     3241 cgttggcaac tgcaccattc ccaagaatga tcaaaatggc agttcccaaa aattcagtgg
     3301 catacttcag tgcccatgtg aaatccattt gctagattct ccttaaaatt ttttagatat
     3361 tccttatttt atcaattttt accctattta gcaactgatt tctggaaata tttttaaaaa
     3421 aataggattc atagtttccc agttcaacag acttgtaagc gtttttattc tttacgaaag
     3481 acctcgttct caaaaccagg aaaaaagccc acatctgtga gcttttcctg gttcctagat
     3541 tctaaaatct attatttttc atcttctttt ttcttgaaaa atcctaatcc tagaccagct
     3601 aacaaggtca tgataccagc actagccaag gtagcattag cttctgtacc tgtgtttggc
     3661 aattcctctc tcttacctgt ctcataagtc ggaacttctg ggtctggatt cactggagtt
     3721 tcagtttttg gagtacctgg ttctggagtt ggtttatctg gtgttggagt ctcttgattt
     3781 ggtagttctg ggtttggtgt cggaacactt ggatgctctg gatttggttg tgggattggt
     3841 ttttctggtg ttgataaacg gtcatacctt accgttattt ctttatcact agagtctgac
     3901 gtaacttctt gtgattcaac tgttggaata tctggatctt tgtacttgtc aatcttacca
     3961 gatataacct cgtcccagtt tcctgttgtc cattcaccgt aggttacaac tcccgtgacc
     4021 ttgttctcag tttttgtacg gcttaaggtt acaggttgaa caacatcttc ttttacattt
     4081 tggttcgtaa ctttatcaac gtaatgaatg atacgcgtta tagtcttcgt ctcagtagag
     4141 gttgctgttt tgggaaccac tgtttcctca acattctcac ggtagtaata gtcaactgtt
     4201 gcaccgtctt ctggtacgca tttgcaggag ttgctaccaa ggtgtatgtt gttttccttg
     4261 tgataactcg gtcttctttg tcctcagttg ttgttttccc ttcaatagtt tttgattctg
     4321 tggtatactc agaacctatc gctaaatcag cttttataac agactctacc aacttctctt
     4381 gcctaccttc tttatagtaa ttggctgtta ctgtagcagt ggttggcgct tcgctttctc
     4441 tataaactaa ggtcactgtt ctaccttcgc ttacaatatt cccagttaaa cttgcagaat
     4501 ttgtatctgc ttctttaaaa gtataatatt ttccgtcagt agtagtcatg ctactgagtt
     4561 ttttatctgt gacattatag ctggtaccaa tcagttgttt tttattggta atgtaggttc
     4621 cgtcactttc tttttctcca attccagtat catttttcaa tgatagcaaa cgcccttgtt
     4681 catcaacata gcgaactttc acattttctg agattagttc tgccaattct gaggtttttt
     4741 tctttttctt gatttcttcg gttattttcc ctttctcttc ttcgggaata tttagttttg
     4801 gaatgatttt ttcaacaacg gttcgtgatg gttccacagt atcttggatg actgaaaagt
     4861 cagctagaat tgggagatta tactgaacac ggtgactttg agtgtttact cctactcttt
     4921 cattattctc tgaaaatact tgtacggtat aagaaacaac atcttttcct aatagaacat
     4981 ccccagtaga gaaatagccg ccttttccta gtttgctatc tccagagccc acttctttcc
     5041 taatcttatc agatagtttt ttaccagtca gtacattcgt tcgcacaatc cctttgtcta
     5101 cccctacaaa gtgggagaac tttttgaact cttcagaacc agatctagcc caaccattat
     5161 taagggcatt tgcttttgta tttgtattct ctctcaaaag tttggcgatt agaattatat
     5221 tcatcggcac ttagagttgc tgctatatct gactcttgaa taccaacttc cttactacca
     5281 tttctagcgg cagtatatgt gtatttatct gtttctactt ctagctgatt atccttaaca
     5341 tgataggtaa ttttctcctg gtataagagt cttcagtagt ttatatactc tgtcatcatc
     5401 tttagcttta gaagcagctg agtttagcca agttggggta ttaggagtat ctttatcaac
     5461 ctgtttcaag acattattca agtcatttgc tagcctattg gttatcctca ctgtgaaagt
     5521 tttcttccag tctgaaccac ttttattaga cttagatatg ctcatgaaat cctggtcaac
     5581 attcaacaca atatcgcctt gttttttagc atcttccgtt gcttctgctt caaattgatc
     5641 atggagtttt tgttgctctg gtgtatagtt tttttattta cacgatttaa tgtactattt
     5701 tttattttag tattaagaac tccattaata cgatttttaa actctgcttc aaaaaatccc
     5761 ttaatttgtt ggtgcgcaag ttcaacattc aaacctttct tatacaagtc ttcattctga
     5821 tacaagtctt cattctgaac gatttcggct acacggtgaa tggtttgatt gtataccgtc
     5881 ttctcttaac cagcagtatc cttagtttta atgactactc gataggttcc tttagaaccg
     5941 ttatagtcat ctagttttaa aaaagcattc caaacattca aaaatagttg gaacttccct
     6001 taatacatcc gtcactttct ttttcaagat agcccaagct tgctcaatag gattcaaatc
     6061 cggtgaataa ggtggtagag gtaagaaaaa gtgtttatct tggatgcaga gttcatccaa
     6121 aatgttcttg ggatgaaaac cagcattgcc catgacaata acatgaggtg tcttcaaagc
     6181 aggtaggagt tgcgttttga accactccac aaagaaatcg cttgtcatgc ttttcttgta
     6241 aatcatggga gctataaact ctccgtctac ttgtcctgca acaattgaag ttcgctcaaa
     6301 acgacgtccg ctaatctttt catagacttt ctcccctcta ggagcccctg cataaggacg
     6361 atagagatag cggtcgattc ctgtttcatc aatataaaca ggtgctaggt gctttaaact
     6421 attaaaattc ttaagaaata aggctacttt tctgggtctt gttccttaaa gctcgtcatc
     6481 tttttttaaa gtgaccttaa tctgctttaa agctgcccat actgaaggaa cagcacaatc
     6541 aaaatgtgcc gcaatttccc gtaaaaaagc atctggatga gcctctacaa aggctttcaa
     6601 ttcctctaaa ggaatctttc ggtttttgac gactcgcttt ttcctctcta tgtgttcttg
     6661 ttcacgtctt tcttttccca cgtgaagaga gttctgacgc caacaccaaa aaacttggct
     6721 gcctcgacat ggctgtgccc ctctttgatg taatctaatg ctcgctgttt aaaatcagta
     6781 ctatatgcca tagctttatt ataatatgct tgtttaaaac taagtaacta tatcaatgtt
     6841 ttgttatgag aagacttact ttttttactg aaattgaatt tttcctagcc tcaacatcat
     6901 ataaccccaa aattcaatct gacctggttt gaagagatgt tcgaagcaga taaggtttta
     6961 ctaaaactaa aaaaaatgat ataattaaac tgattaaatg ttttaaaaat aaaggagaat
     7021 ttgaatgtct taccaagaaa attaccagaa atgggttgat tttgtggagc ttcctgacta
     7081 ccttcgtcaa gatttggaaa atatggacga aaaaactaag gaagatgcct tctatacaaa
     7141 tcttgaattt ggtactgcag gtatgcgtgg cttggttggt gctggtacaa accgtatcaa
     7201 catctacgtt gttcgccaag ctactgaagg attggctcgt ttgattgagt caaaaggtgg
     7261 aaacgagaaa gaacgcggtg tagcaattgc ctacgatagc cgtcacttct cacctgagtt
     7321 tgcctttgaa tctgcggcag ttcttgctaa acacggcatc aaatcttacg tatttgaaag
     7381 ccttcgtccg actccagaac tatcatttgc agttcgtcat ctcaactgtt tcgcaggtat
     7441 catggtcaca gccagccaca accctgctcc atttaacggt tacaaggttt acggtgaaga
     7501 cggtggacaa atgcctccac acgatgcgga cgctttgact acttatatcc gtgcaatcga
     7561 aaacccattt gcagttgaag ttgctgatgt ggaaactgaa aaagcttctg gcttgattga
     7621 agttatcggc gaagctgttg acatagaata ccttaaagag gttaaggaca taaacatcaa
     7681 cccagccttg attgaagaat ttggtaaaga catgaagatt gtctacacac cacttcatgg
     7741 tactggtgaa atgttggctc gtcgtgctct tgcccaagca ggatttgact ctgttcaagt
     7801 tgttgaagcg caagcaactg ctgacccaga cttttcaact gtaacatctc caaacccaga
     7861 aagccaagca gcctttgccc ttgctgaaga acttggtcgt caagttggtg cagatgttct
     7921 tgtcgcaact gacccagacg ctgaccgtgt tggtgttgaa gttcttcaaa aagatggtag
     7981 ctacctcaac ctttcaggta accaaatcgg tgctatcatg gctaaataca tcttggaagc
     8041 tcacaaaaac gctggaactc ttcctgaaaa tgccgctctc tgcaaatcta tcgtttcaac
     8101 tgacttggta acgaagattg ctgaaagcta cggcgcaact atgttcaacg tcttgacagg
     8161 tttcaaattt atcgctgaaa aaatccaaga attcgaagaa aaacacaacc acacttacat
     8221 gatgggattt gaagaaagct tcggttactt gattaaacca tttgtacgtg ataaagatgc
     8281 catccaagcc gttcttgtag ttgctgaact tgctgcctac taccgttctc gtggtttgac
     8341 acttgctgac ggtatcgaag aaatctataa agaatatggc tactacgcag aaaaaacaat
     8401 ctctgttact ctttcaggtg tcgatggtgc tgaacaaatc aaagcgatta tggctaaatt
     8461 ccgcaacaat gctccaacag aatggaacgc aacagctatc actgtcgtag aagacttcaa
     8521 ggcacaaact gctactgttg ctgacggtac tgttacaaac ttgacaactc ctccaagtga
     8581 tgtgttgaaa tacacacttg ctgacggttc atggattgcc gttcgccctt caggtacaga
     8641 accaaaaatc aagttctaca ttgcagttgt aggggaaacc aacgaagaat cacaagctaa
     8701 gattgctaac atcgaagcag aaatcaatgc atttgtaaaa taaagaccta taaaagatga
     8761 gaccaaccaa cctcatcttt tttcgtatca aatctaagca attttagaaa ctttatggca
     8821 tactagactt atcaatgaaa gcgaggtaat ctcatggaac aatttttaga taacatcaaa
     8881 gaccttgaag tcactacagt tgtgcgtgcg caagaagctc ttgataaaaa agaaactgca
     8941 accttcttta tcggtcgcaa aacttgccct tactgccgta aatttgcagg tacattgtca
     9001 ggtgtcgtag ctgaaaccaa agctcacatt tacttcatca atagtgaaga accaagccaa
     9061 ctcaatgatt tgcaagcatt ccgctcacgc tatggaatcc caactgtacc aggttttgtt
     9121 catattacag atggacaaat caacgtccgt tgcgactctt caatgtcagc acaagaaatc
     9181 aaagatttcg caggattgta aaaaatcagc taacaagtct taaaatcaca aaaacgcata
     9241 atgtaaggtg caaaaacctt gatattatgc gttttattgt tcctaaaaca gttacatcag
     9301 tatttcatta cagaacattt taattttgga agtcaaatca atttttaaca atgttttaga
     9361 aatcacagtg ggctattcca tattcaatta tccatattca atcgctatta tcgttttcct
     9421 agattacaaa aaaaactacg ttttgctaat caaaacgcag tttcagccac tattttatct
     9481 tactgtcctt cttttacttc tttggttgct acatcttctc caaaccattt ttggctgatt
     9541 tcttggaact tgccgtcctt gtaaagacta gaaaaagctt catttatctt cttaaccaag
     9601 tttgtatctt ccttacgggc tccaaccgca aaagcttctg tttctagtcc aactgtaaag
     9661 acattataat cgtttaaaac accttctgct tctaaataat agtttgcata gacacggtca
     9721 atcaatagac catcaattcg atcgtttttc aaatcaatca aggcttcatt aaaggtttgg
     9781 tattgattcg cttccttatt agcgacaata ttcttcaaaa tttctggatt tgcttcaaag
     9841 tccgcataac cagatgaacc agcttgagct cctaatgtct ttccagtcat atcctttgca
     9901 gtcgtgatac cagatgattt cttcgtaacc aatacctgct cattcttcat atatgagtta
     9961 ctgaaagcca ccttttcacg gcgttcgtct gtagcggaat agccattcca aatcagatca
    10021 atcgttcctt ttgtcaattc agcttctttc aaatcccaat caatcggttg ccaatttacc
    10081 gtgattccgt atttttcaaa aacagctgta gctaaatcaa tatcaaatcc tgcataagaa
    10141 ccatctttct gagcaaatcc cattggaaca aaagtactat caaatccaat agtaatagac
    10201 ttgttagact ggtactttga ccaattatct ccactagttt cgctagaatt tttcccacaa
    10261 gctactaaga acaaagcagt catcagactg actaatacaa gcatccattt tttcatccat
    10321 ttttcctcct atttaggttc tactttcaat aatacatctg cgatattttc agcaaactgc
    10381 aaatcatggg taaccacaat ctgggtcatc ccaagttccc tattttgcaa gattagcttc
    10441 tccacttcca aacgtaattc tggatccagg gcagaagttg gttcatcgta gccaatgatt
    10501 tctgggtcaa tcatcatagc acgcgccaaa gccacccgct gcttttgccc accagatagt
    10561 gagaaaggat aggcctctgc gtgtcctcct agtcctaact gttccaagag tccactcgcc
    10621 ttcttctcag cctcttcctg cttcattccc atggtcttca caggcgataa agtcaaattt
    10681 tccagaactg atagatgagg aaatagttga aaatcttgga agacaaatcc cagtagattg
    10741 cgcttctgca attcatccag ctctaaaggt tgtccattat aaaagatttg ccctgaatca
    10801 atggtttcaa gacctgcaag catacgtaaa agagttgtct tacctccacc agaaggtcca
    10861 acgatagcca ggatttgctt ttcaggaata cttagactga aattagacag gatttgtttg
    10921 tctccaaaga ctttattgat atttcgtaat tctaacatgg cagcctccta tctataataa
    10981 ctatacttct tctcaacttt tttggaaata attgtcacaa tcccaatcaa aatcaaataa
    11041 atggctcctg ccaagaacat aggaactaga ctagcatcgc ggttagcagc tgttcgacta
    11101 gccaagataa ggtctgaaat tccgagagca tagaccaaag aagtatcctt gaccaaactc
    11161 ataacttcat taaagacact aggaagaacg atcttggtca cttggggcaa gataatatag
    11221 cgcactctgt caaaagggct aaacttcaag accttggcgg cctcatactg tcctcttgga
    11281 atagtgtcaa ttcccccacg gaaaatttct gcaaagtaag ctgcatagtt gagaacaaag
    11341 gcaataatag ctgcaggaag gcggtctaaa cgaatcccaa tacttgggag cacataatag
    11401 ataaaaatca gttgcaagag taagggggtt cctcgcataa cccagatgta aatgttaatc
    11461 agataatgga ggggcttcca atggacttgc aaggcaaagg caatcaaaac gcccaaggga
    11521 atcgaaaata gcaagaccag tgcaaagacc tgtacagtcg tgctcgcacc attcagtaaa
    11581 ctcggtaata tctcaaataa ataagacata ctgcacctcc taaaagtaat tgttcctatt
    11641 atagcatgaa taaacaagat taccaagtat ttttgtaaaa agattctacc atatacaaat
    11701 aaaaaccgta gctatcaaaa gagataaact acagttctat atgttt
//
LOCUS       DAVHEW010000062        11741 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVHEW010000062 DAVHEW010000000
VERSION     DAVHEW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.062"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            72..1343
                     /locus_tag="VVL53_001485"
     CDS             72..1343
                     /locus_tag="VVL53_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4183642.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1454..2746
                     /locus_tag="VVL53_001486"
     CDS             1454..2746
                     /locus_tag="VVL53_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4183643.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2748..3590
                     /locus_tag="VVL53_001487"
     CDS             2748..3590
                     /locus_tag="VVL53_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4183644.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3843..4643
                     /locus_tag="VVL53_001488"
     CDS             3843..4643
                     /locus_tag="VVL53_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU4183645.1"
                     /translation="MLPYPFSYFSSIWGVRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASFLLYITKRSRLFSFNTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4653..5639
                     /locus_tag="VVL53_001489"
     CDS             4653..5639
                     /locus_tag="VVL53_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4183646.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            5775..5855
                     /locus_tag="VVL53_001490"
                     /pseudo
     CDS             5775..5855
                     /locus_tag="VVL53_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6065..7006)
                     /locus_tag="VVL53_001491"
     CDS             complement(6065..7006)
                     /locus_tag="VVL53_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU4183647.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(6984..8747)
                     /gene="aspS"
                     /locus_tag="VVL53_001492"
     CDS             complement(6984..8747)
                     /gene="aspS"
                     /locus_tag="VVL53_001492"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU4183648.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITESLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(8983..9063)
                     /locus_tag="VVL53_001493"
                     /pseudo
     CDS             complement(8983..9063)
                     /locus_tag="VVL53_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9099..9329)
                     /locus_tag="VVL53_001494"
     CDS             complement(9099..9329)
                     /locus_tag="VVL53_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183649.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(9347..9988)
                     /locus_tag="VVL53_001495"
     CDS             complement(9347..9988)
                     /locus_tag="VVL53_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183650.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(10000..10680)
                     /locus_tag="VVL53_001496"
     CDS             complement(10000..10680)
                     /locus_tag="VVL53_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU4183651.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVAIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            complement(10736..10933)
                     /locus_tag="VVL53_001497"
     CDS             complement(10736..10933)
                     /locus_tag="VVL53_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183652.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(10933..11139)
                     /locus_tag="VVL53_001498"
     CDS             complement(10933..11139)
                     /locus_tag="VVL53_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183653.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(11149..11463)
                     /locus_tag="VVL53_001499"
     CDS             complement(11149..11463)
                     /locus_tag="VVL53_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183654.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
BASE COUNT         3532 a         2618 c         2143 g         3448 t
ORIGIN      
        1 ctaagctcat aaaggaaaac gtttgcgttt ccttatcaat aaaacttgct attctttagg
       61 aggaatacac tatgtcatct aaatttatga agagcgctgc ggtgcttgga actgctacac
      121 ttgctagctt gcttttggta gcttgcggaa gcaaaactgc tgataagcct gctgattctg
      181 gttcatctga agtcaaagaa ctcactgtat atgtagacga gggatataag agctatattg
      241 aagaggttgc taaagcttat gaaaaagaag ctggagtaaa agtcactctt aaaactggtg
      301 atgctctagg aggtcttgat aaactttctc ttgacaacca atctggtaat gtccctgatg
      361 ttatgatggc tccatacgac cgtgtaggta gccttggttc tgacggacaa ctttcagaag
      421 tgaaattgag cgatggtgct aaaacagacg acacaactaa atctcttgta acagctgcta
      481 atggtaaagt ttacggtgct cctgccgtta tcgagtcact tgttatgtac tacaacaaag
      541 acttggtgaa agatgctcca aaaacatttg ctgacttgga aaaccttgct aaagatagca
      601 aatacgcatt cgctggtgaa gatggtaaaa ctactgcctt cctagctgac tggacaaact
      661 tctactatac atatggactt cttgccggta acggtgctta cgtctttggc caaaacggta
      721 aagacgctaa agacatcggt cttgcaaacg acggttctat cgtaggtatc aactacgcta
      781 aatcttggta cgaaaaatgg cctaaaggta tgcaagatac agaaggtgct ggaaacttaa
      841 tccaaactca attccaagaa ggtaaaacag ctgctatcat cgacggacct tggaaagctc
      901 aagcctttaa agatgctaaa gtaaactacg gagttgcaac tatcccaact cttccaaatg
      961 gaaaagaata tgctgcattc ggtggtggta aagcttgggt cattcctcaa gccgttaaga
     1021 accttgaagc ttctcaaaaa tttgtagact tccttgttgc aactgaacaa caaaaagtat
     1081 tatatgataa gactaacgaa atcccagcta atactgaggc tcgttcatac gctgaaggta
     1141 aaaacgatga gttgacaaca gctgttatca aacagttcaa gaacactcaa ccactgccaa
     1201 acatctctca aatgtctgca gtttgggatc cagcgaaaaa tatgctcttt gatgctgtaa
     1261 gtggtcaaaa agatgctaaa acagctgcta acgatgctgt aacattgatc aaagaaacaa
     1321 tcaaacaaaa atttggtgaa taaaaaattt gttcaagggg ggtggaaatc aaatccccct
     1381 ttgaatttat caatagagac acaaataatt tagctttctt ataaaaaagt agtatcctat
     1441 gaaaggagtt aatatggaaa agcaacaacc tagtaaagca gccctgctgt ctatcattcc
     1501 tgggttagga cagatttaca ataaacaaaa agccaaaggt tttatcttcc ttggtgtaac
     1561 catcgtattt gtcctttact tcctagcact tgcaacccct gaattgagca acctcatcac
     1621 tcttggtgac aaaccaggtc gtgataattc cctctttatg ctgattcgtg gtgccttcca
     1681 tctaatcttt gtaatcgttt atgtactctt ttatttctca aatatcaaag atgcacatac
     1741 gattgcaaaa cgcattaaca atggaattcc agttccacgc acactcaaag acatgatcaa
     1801 agggatttat gaaaatggct tcccttacct cttgatcatt ccatcttatg ttgccatgac
     1861 cttcgcgatt atcttcccag ttatcgtaac cttgatgatc gcctttacca actacgactt
     1921 ccaacacttg ccaccaaaca agttgttgga ctgggttggt ttgaccaact ttacaaacat
     1981 ttggagcttg agtaccttcc gttctgcctt tggttctgtt ctttcttgga ctatcatttg
     2041 ggctttggca gcttctactt tacaaatcgt aattggtatc ttcacagcta tcattgccaa
     2101 ccaaccattt atcaaaggaa aacgtatctt tggtgttatt ttccttcttc cttgggctgt
     2161 cccagccttc atcactatct tgacattctc aaacatgttt aacgatagtg tcggtgctat
     2221 caacactcaa gtattgccaa tcttggctaa attccttcct ttccttgatg gagctcttat
     2281 tccttggaaa acagacccaa cttggactaa gattgccttg attatgatgc aaggttggct
     2341 cggattccca tacatctacg ttctgacctt gggtatcttg caatctattc ctaacgacct
     2401 ttacgaagca gcttatattg acggtgccaa cgcttggcaa aaattccgca acatcacttt
     2461 cccaatgatt ttggctgttg cggcacctac tttgattagc caatacacct tcaactttaa
     2521 caacttctct atcatgtacc tcttcaatgg tggaggacct ggtagtgtcg gaggtggagc
     2581 tggttcaacc gatatcttga tctcatggat ctaccgtttg acaacaggta catctcctca
     2641 atactcaatg gcggcagctg ttaccttgat tatctctatc attgtcatct caatctctat
     2701 gatcgcattc aagaaactac acgcatttga tatggaggac gtctaagatg aataactcaa
     2761 ttaaactcaa acgtagactg actcaaagcc ttacttacct ttacctgatt ggtctatcaa
     2821 ttgtaattat ctatccactg ttgattacca ttatgtcagc ctttaaagca ggtaacgtct
     2881 cagcctttaa actagatact aatatcgacc tcaattttga taactttaaa ggcctcttca
     2941 ctgaaacctt gtacggtact tggtacctca acactttgat tatcgcctta attaccatgg
     3001 ctgttcaaac aagtatcatc gtacttgctg gttatgctta cagccgttac aacttcttgg
     3061 ctcgtaaaca aagtttggtc ttcttcttga tcatccaaat ggtgccaact atggccgctt
     3121 tgacagcctt cttcgttatg gcgcttatgt tgaacgccct taaccacaac tggttcctca
     3181 tcttcctcta cgttggtggt ggtatcccga tgaatgcttg gctcatgaaa ggctacttcg
     3241 atacagtgcc aatgtcttta gacgaatctg caaaactaga cggtgcagga cacttccgcc
     3301 gcttctggca aattgttcta ccacttgttc gcccaatggt tgccgtacaa gctctctggg
     3361 ccttcatggg acctttcggg gactacatcc tctctagttt cttgcttcgt gagaaagaat
     3421 actttactgt tgccgtaggt ctccaaacct tcgttaacaa tgcgaaaaac ttgaagattg
     3481 cctacttctc agcaggtgct atcctcatcg cccttccaat ctgtattctc ttcttcttcc
     3541 tacaaaagaa ctttgtttca ggacttacaa gtggtggcga caagggataa tttatccccg
     3601 ccaccctttt tcattttata ctcttcgaaa atctcttcaa accacgtcag ctttatctcc
     3661 aacctcaaag ttgtgctttg agcaacctgt ggctagtttg cactttgatt ttcattgatt
     3721 attagcaatt gtcactgtaa ataatatcct tgtagcaagc aatttttctc ctagacttga
     3781 aataaagcgc atttctctat ataataatac tcatatagaa aacacctttt agaaagatac
     3841 ctatgcttcc atatccattt tcctattttt caagtatttg gggggttcgt aagcccctgt
     3901 ccaaacgttt cgagctcaac tggtttcaac ttctctttac cagtatcttc cttatcagct
     3961 tgtctatggt acccattgct atccaaaaca gctcccagga gacctatccg ctagaaactt
     4021 ttatcgataa tgtctatgaa cctctgacag ataaggttgt ccaggatctc tctgaacatg
     4081 ctacaattgt cgatggcaca ttaacttata ctggaacagc tagtcaagcc ccttctgttg
     4141 tgattggtcc aagtcaaatc aaggaattac ctaaggactt gcaactgcat ttcgatacaa
     4201 atgagctagt catcagcaag gaaagcaagg aactgacccg catctcttac cgagccattc
     4261 agactgagag tttcaaaagc aaagacagct tgacccaagc aatttctaaa gactggtacc
     4321 aacaaaatcg tgtctatatc agcctcttcc tagttctcgg tgcgagcttc ctctttggtt
     4381 tgaatttctt tatcgtctct cttggagcta gctttctcct ttatatcacc aaaagatcac
     4441 gcctcttttc atttaatacc tttaaagagt gctaccattt tatcttgaac tgtttaggat
     4501 tgccgactct gattacactt attttgggat tatttggcca aaatatgaca accctgatta
     4561 ctgtacaaaa tattcttttt gttctgtatc tggtcactat cttttataaa acacatttcc
     4621 gtgatccaaa ttaccataaa taggagattt ttatgcccgt tacgattaaa gacgtggcca
     4681 aggctgctgg tgtttcgcct tcaaccgtaa cccgtgttat tcaaaataaa tcaaccatta
     4741 gcgacgaaac aaaaaaacgt gttcgcaaag ctatgaagga actcaactac cacccaaacc
     4801 tcaacgctcg tagcttggta agcagctata ctcaggttat cggattagtt cttcctgatg
     4861 actcagacgc cttctaccag aatcctttct ttccatcggt tctacgtggc atctctcaag
     4921 tcgcatctga aaaccactat gccattcaga tagcaacagg gaaagatgag aaggagcgtc
     4981 tcaacgctat ttcacaaatg gtctacggca agcgtgtaga tgggctaatt tttctctatg
     5041 cccaagaaga agaccctctc gtaaaactcg tcgcagaaga acagttcccc ttccttatct
     5101 taggtaaatc tctatctcct ttcatcccac ttgtcgacaa cgacaatgtt caagctggtt
     5161 ttgatgcgac tgaatatttc atcaaaaaag gctgcaaacg cattgccttt atcggaggaa
     5221 gtaaaaagct cttcgtgacc aaagaccgtt taacaggcta tgaacaggcg cttaaacatt
     5281 acaaacttac cactgacaac aatcgcatct actttgccga cgagtttctg gaagaaaagg
     5341 gctataaatt tagcaagcga ttattcaagc acgatccaca aattgatgct atcatcacaa
     5401 ccgatagcct cctagctgaa ggtgtttgta actatattgc caaacaccag ctggatgtcc
     5461 ctgttctcag ctttgactcg gttaatccca agctcaactt ggcagcctat gtcgatatca
     5521 atagtttaga gcttggtcgt gtttcccttg aaactattct ccagattatt aatgataata
     5581 aaaacaataa acaaatttgt taccgtcaat tgatcgccca caaaattatc gaaaaataag
     5641 agactgggca aaaagtcgtt aaaagcaaaa acgcatacta tcaggtattg aaaaaacttg
     5701 atactatgcg ttttattgtg ggaagattta cttccttttc tactgaaatt gagtcttttc
     5761 ccaagatctt tttatactca atgaaaatca aagtgcaaac taggaagcta gccgcaggtt
     5821 gctcaaaaca ctgttttgag gttgtagatg aaactgacga agtcagctca aaacatggtt
     5881 ttgaggttgc agatagaact gacgaagtca gctcaaaaca tggttttgag gttgcagata
     5941 gaactgacga agtcagctca aaacatggtt ttgaggttgc agatagaact gacgaagtca
     6001 gtaacatata tacggcaagg tgaagctgac gtggtttgaa gagattttcg aagagtatta
     6061 atcactaatt atctatctca acaaatcttc ctagaatatg aacattttcc gagacagaga
     6121 caaaggagct tggatccact tgtgtcataa tctgtttaaa ttcattaaac tctgcacgtg
     6181 taatgacagt gattaaaact gcctttctct cgtgattata ggttccttct gcatcgtgga
     6241 tcatggttgc tccgcggtgc aattttttat ggattttttc aattaccttc tctggatgat
     6301 ttgtcacaat catggcctgc atacgctttt gcttagtaaa gactgcgtct gtcacacggc
     6361 tagagacaaa gatggtaatc atagaataaa gagcgtattt ccaaccaaag gtcaaacctg
     6421 ctatcagcat gatagttcca tttaccaaga aagaaatact accgacattc ttacccgttt
     6481 tcttacgaat agtcaggctg acgatatccg tcccaccact ggagatattg tttcgaagag
     6541 caaaaccaat ccccaaaccc ataacaacac ccccaaaaag ggaattgata atgggatcct
     6601 ctgtcaaggt tgccacaggg acaaactgga taaagaagga actcatagat accgtgataa
     6661 aggtaaagac ggtgaactta tggccaatct gataccaagc taagaccatc aaagggaagt
     6721 taatggcgta gaagcttagc gaaatcggaa tatgaaaacc aaaccagtga ttactcaagg
     6781 cagagataat ctgtgccaga cctgttgcac cactcgaata cacatgccct ggttggaaaa
     6841 agaaattaac tgctactgct gataaaaaac catagaccag agaggccgaa atcttctcat
     6901 catacttttc tcgagagata ctttgtaaga cacgtaaaat ttttatctga taagcaaagc
     6961 ggcgcagata atagcgccac cgcttaattc gttttgtttg tttcatcttc ttctacttgt
     7021 aagctgagtt cctctagttg tttgagagcg actgttgatg gagcttgtgt cattgggtca
     7081 gttgccttgt tgttcttagg aaaggcaatg acttcacgga tattttcttc tccagcaagc
     7141 aacatgacaa aacggtcaag cccgatagcc aaaccaccgt gtggtgggaa accatagtcc
     7201 atggcttcaa gaaggaaacc aaactggtca ttggcttctt cagttgagaa accaagagcc
     7261 ttgaacatgc gttcttgaag gtctttttgg ttgatacgaa ggctaccacc accaagctca
     7321 taaccgttca agacgatatc gtaagcaatg gcacgaacct tagccaaatc accttctaat
     7381 tcatgagcag tctcttcctg tggaagtgtg aaaggatggt gggcgctcat gtagcggcct
     7441 tcttcttcag accattcaaa catcggccag tcaaccaccc aaaggaagtt gaacttatca
     7501 ttatcaatca agccaagctc tttagcaata cgtccacgaa gggcacccag tgttgcatta
     7561 gccacttcaa gcgtatccgc cacaaagaga accaagtcct tatcttcaag aacaagcgct
     7621 gttgtcaatt cttcttggat accagtcaag aacttggcaa ctggtccgtt taattctcca
     7681 tcaaccacct tgacccaagc aagacctttg gcaccatact gtttggctac ttccgtcatc
     7741 ttgtcgatgt ctttacgtga atagttgtcc gcagctcctg tgaccacaat cgcttttaca
     7801 gcaggtgctt ctgaaaagac tttaaagtct acacctcgga ccacttctgt caagtcctga
     7861 agcaacatgt caaaacgagt atctggcttg tcagaaccgt aaagagccat agcatcatcg
     7921 tatttcatac gagggaatgg tagcgttact tcgatgcctt ttgtttcctt catcacgcgc
     7981 gcgatcaagc tttctgtaat atcttggatt tcttgctcag taaggaagga cgtttccaag
     8041 tcgacctgag taaattcagg ctggcggtct ccacgcaagt cctcgtcacg gaaacattta
     8101 acgatttggt agtaacggtc aaaaccagca ttcatcaaga gctgtttcgt gatttgtgga
     8161 ctttgaggaa gagcgtaaaa atgcccctta ttaacacgag acggcactaa ataatcacgc
     8221 gccccttcag gcgttgactt agaaaggaat ggtgtctcca cgtcgataaa ctccaactca
     8281 tccaagtagt tgcggataga gtgggtcacc ttggcacgaa gtttaagatt ttccaacatt
     8341 tctggacgac gaaggtcaag gtaacggtaa cgcaaacgtg tatcgtcatt tgcctcaatg
     8401 ccatccttaa tctcaaatgg tgttgtctta gctgtgttaa gcacaataag agctgtcacg
     8461 tttaactcaa ccgcaccagt tggcaactta tcattggctt gctcacgcgc agcgacctga
     8521 ccagtcacct caataacaaa ttcgctacga aggctttcag ctgttgccat aacctctgca
     8581 gatacttttt cagggttgat aaccaactgc atgattcctt cacggtcacg aagatcgata
     8641 aagatcaaac caccaaggtc acgacgacgg ccaacccatc ctttcaaggt tatttcttgt
     8701 ccgatgtgtt cctcacgaac acgaccagca tacatactac gtttcattat ttctctcctc
     8761 ttttattctg ttactatttt accataaaag cgcaggctct tcatgaaaat catcagaaaa
     8821 gtttgccagt ctttaaaagt caggtgaaag ccctaaaaat tagcgctaat actcttcgaa
     8881 aatctcttca aaccacgtca gcgtcgcctt accgtatgta tggttactga cttcgtcagt
     8941 ttcatctaca acctcaaaac catgttttga gctgacttcg tcagttctat ccacaacctc
     9001 aaaacagtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga
     9061 gtataataca aaaatccgat gaacttcacc ggactctttt attttgaatt tttgcctgct
     9121 ttacgctttt cagcgatttc ggctgccttt cgaggcaaga caatttccgt tatgtaagcc
     9181 gtcccaaaac gcagtacacc tgcaatagga gcaaagacaa ctgctagata gttatagaag
     9241 aaatcgcctt tgaaggcata agctagcgct ccaatgatga aaaatagaac gactgcctga
     9301 atcactgcta ataaaattac tcgtttcatg tgacctcctg actctattat agcatgagaa
     9361 tcatcaaaaa gccgactaaa ttattcaaag cgtgaagaga aatactgtag accagacctt
     9421 ttctgctaat gtaagccaaa cccaaactaa aaccaaggct aaaatagaca aaaaattgtt
     9481 gcacatcacc tggaaaatga atcaaggcaa atagaagact agataccaga agaaaaatca
     9541 gggttcgttt actattgtcc tgcttaggaa agagatagcg tgctaacatc cctctaaaaa
     9601 caatctcttc cgtcaaagga gcaaaaataa ccacagcaaa gaatgagaaa agtggttgag
     9661 acaaggtcaa gtctgtcgct atttgctgat ttactgaagg atcatctggc aagaagaatt
     9721 gaacgaccag agataagaac caaaccaaga caggaagcca aataaatcga ttaaagccgc
     9781 tcttctcaat atgaacagga gccttctgat accatttgta aatgccgtac acatatactc
     9841 cagccaaggc cacatagagt agagtaacag catagggtga agcgcctaaa gcaagcgacg
     9901 cagtcgcgag cccctgaata aagccataga taaataaaaa ggatagaagg gctagaagaa
     9961 tccagccaag gtttttaagt aatttcatag ataactcctt tatttgaaat aacgttttac
    10021 cataggtaac tgcatcacat tgatataaac atggatggct cctacaagca agaaagctag
    10081 taactgaatc tctcctgtca agaaagaaat gataataaga aaaatatata aggctggtaa
    10141 gacatattgg tgtaattgga ataaaattcg aaaactctgt tccaaattag cctgacgctc
    10201 cccttcatca taagaattta tatagttcaa gacatccttt ggtgtagcga aaaattccaa
    10261 atcaaactga cgaacaatcg caatggtttt aaaaagagat ttttgagcga ctaagaatac
    10321 cacaaagagt aagaaagaaa ggaaaaatgt ttgagggttt gtatgcaata taatcacctc
    10381 acttaatgaa ataaaaatag ccaatggaat cgctacacct gtaatattaa aagcaatggt
    10441 tccaaactca agattccgat acatttgcac ataataggtt tcattcagat cgtcatccat
    10501 ttcctcttga tacaaggaat gaaattttct gcttttcttt aagaaattga aagtcaaaaa
    10561 catactaatg aaacctatca gtaaacaaat agctgatatc catggcatca aggcttttac
    10621 atctaaaata atttcgtggg attcgacacg tgccttaaac atccctacaa acatgcccaa
    10681 gaacccccca agacaataga catcaaaaat aacaatctac gtttcttttt catattcatt
    10741 ctcctttttc acttgctaga tttttggatt tcttttcaat ccattcaatt actgggatga
    10801 gagcaaagta gacccaaaca aattggtcgc tttgataggg attaaaccag cttaggtcca
    10861 tcccaatcag tagaaatacg ctgactaata aagctatgac cactacataa taaatcactt
    10921 tatacttgtt catcactcgt cctcctccaa acgaaatacc gattcgactg tttcgttgaa
    10981 aatttgagat attttcaggg caatgataat ggatggggtg tactcatccc gttctagtag
    11041 gctaatggtc tgtctggaaa cccctgccag tttggctagg tcggtttgat tgagaccatc
    11101 gcgagctcga agctctttta gacgattttt tagttgcatg ttacacacct actctccgtc
    11161 aaattcaacg gtttggatat cctcaatacg ttgcaacttg aatttttctt ttcccgtatt
    11221 atctacacgt cgtagcttta cccattcctc atcaacatcc acaacttccc agttatctgg
    11281 cccaatatac actcccgtta taattggttc ctttccaatc atttcttgta ataatctcga
    11341 catttctgcg tttcctttct cttttcgctc aagtcttttg attttattct ctagtttctt
    11401 gattttttta gaattattag aataaaagaa aatcataaat agtataaatc ctagtaccca
    11461 cattataact cctttctgct tcctatttct taacttgaat tcattgtaac atatcttttt
    11521 ctttttgaca agtatagttg tcaaaaaaat tatgattttt gtcattttgc aaaagaaaaa
    11581 ggtcaggagt aggttcctga ccactttatc tatcattaat actcttctaa aatctcttca
    11641 aaccacgtca gcttcacctt gccgtaggta tggttactga cttcgtcagt ttcatctaca
    11701 acctcaaaac catgttttga gctgacttcg tcagttctat c
//
LOCUS       DAVHEW010000063        11632 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVHEW010000063 DAVHEW010000000
VERSION     DAVHEW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.063"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            126..884
                     /locus_tag="VVL53_001500"
     CDS             126..884
                     /locus_tag="VVL53_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4183655.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAILSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            923..1687
                     /locus_tag="VVL53_001501"
     CDS             923..1687
                     /locus_tag="VVL53_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU4183656.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1700..2377
                     /locus_tag="VVL53_001502"
     CDS             1700..2377
                     /locus_tag="VVL53_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4183657.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2387..3046
                     /locus_tag="VVL53_001503"
     CDS             2387..3046
                     /locus_tag="VVL53_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4183658.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVT
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3304..4494
                     /locus_tag="VVL53_001504"
     CDS             3304..4494
                     /locus_tag="VVL53_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU4183659.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4815..5696)
                     /locus_tag="VVL53_001505"
     CDS             complement(4815..5696)
                     /locus_tag="VVL53_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU4183660.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5837..7165)
                     /gene="vncS"
                     /locus_tag="VVL53_001506"
     CDS             complement(5837..7165)
                     /gene="vncS"
                     /locus_tag="VVL53_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU4183661.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(7162..7818)
                     /gene="vncR"
                     /locus_tag="VVL53_001507"
     CDS             complement(7162..7818)
                     /gene="vncR"
                     /locus_tag="VVL53_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU4183662.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7867..7950)
                     /gene="pep27"
                     /locus_tag="VVL53_001508"
     CDS             complement(7867..7950)
                     /gene="pep27"
                     /locus_tag="VVL53_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU4183663.1"
                     /translation="MRKEFHNVLSSDQLLTDKTPARDYNRK"
     gene            complement(8130..9509)
                     /gene="vex3"
                     /locus_tag="VVL53_001509"
     CDS             complement(8130..9509)
                     /gene="vex3"
                     /locus_tag="VVL53_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU4183664.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9561..10208)
                     /gene="vex2"
                     /locus_tag="VVL53_001510"
     CDS             complement(9561..10208)
                     /gene="vex2"
                     /locus_tag="VVL53_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU4183665.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10221..11498)
                     /locus_tag="VVL53_001511"
     CDS             complement(10221..11498)
                     /locus_tag="VVL53_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183666.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3268 a         2610 c         2207 g         3547 t
ORIGIN      
        1 tgactgtccc gatcgatttg tcctgttctt atttcactat agtgaatttt ttcagatatg
       61 gtttgacaag aacattcttt tattgtagaa tcgtgttaca aaatctaaca taaatgagat
      121 ctctcatggc tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccacgcac
      181 tccgcaacat caatctccgt tttgaaaaag gacaagttgt tgtcctgctt ggaccttctg
      241 gctctgggaa gtccactctt atccgtacga tcaatggttt agaggctgtt gacaaaggaa
      301 gtctcctagt caatgggcac caagttgctg gtgccagcca gaaagatttg gtacctcttc
      361 gcaaggaagt cggcatggtt tttcaacatt ttaaccttta tccacacaaa acggtgttag
      421 aaaacgtgac acttgcaccc attaaagttc taggaattga taaaaaagaa gctgaaaaaa
      481 ccgcccaaaa atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca
      541 tactatctgg tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg
      601 aactcctcct ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc
      661 tagcagttat gcagaaactg gcgcatgatg ggatgaacat gatcatcgtt acccacgaaa
      721 tgggctttgc tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag
      781 tagatacgac agatgtcgat aacttttttg acaatccaag caaacctcgt gcccaacaat
      841 tcctcagcaa aattatcaac cacgaaagtg acaaagtcaa ataaggaggc gcctatgaaa
      901 aaaattcttt ttatcagcat tattgattag ccttttcggc cttgctgctg ccaaaccagt
      961 ccaggctgat actagtatcg cagacattca aaaaagaggc gaactggttg tcggtgtcaa
     1021 acaagacgtt cccaattttg gttacaaaga tcccaagacc ggtacttatt ctggtatcga
     1081 aaccgacttg gccaagatgg tagctgatga actcaaggtc aagattcgct atgtgccggt
     1141 tacagcacaa acccgcggcc cccttctaga caatgaacag gtcgatatgg atatcgcgac
     1201 ctttaccatc acggacgaac gcaaaaaact ctacaacttt accagtccct actacacaga
     1261 cgcttctgga tttttggtca ataaatctgc caaaatcaaa aagattgagg acctaaacgg
     1321 caaaaccatc ggagtcgccc aaggttctat cacccaacgc ctgattactg aactgggtaa
     1381 aaagaaaggt ctgaagttta aattcgtcga acttggttcc tacccagaat tgattacttc
     1441 cctgcacgct catcgtatcg ataccttttc cgttgaccgc tctattctat ctggctacac
     1501 tagtaaacgg acagcactac tagatgatag tttcaagcca tctgactacg gtattgttac
     1561 caagaaatca aatacagagc tcaacgacta tcttgataac ttggttacta aatggagcaa
     1621 ggatggtagt ttgcagaaac tttatgaccg ttacaagctc aaaccatcta gccatactgc
     1681 agattaagga ggacacccca tgacagattt atcatcttgg acagcctatt ttcaggattt
     1741 tggacaattt ttcaatggtt tcctcttcac ccttgcccta gcggttggtt cctttatcct
     1801 cgctatggtt ttgggcatct tctttggtgc catgtcaacc agtaaacgtc caattttacg
     1861 cattttagct cgcatctttg ttgaatttta ccaaaatact cccctcttgg tgcagtttgt
     1921 tattgttttt tatggcctac ctcttatcag tgaccatatc atcatgattc caatttattg
     1981 gacagccgtt ctctgcgtgg gactctatca cggcgcttat atcgctgagg ttattcgttc
     2041 agggattcag tctattccta gtggtcagat ggaggccgcc ttgtcgcaag gttttaccta
     2101 tatcagtgcc atgcgcttga ttatcttgcc tcaggccttc cgcatcattc tccctccatt
     2161 gaccaaccaa attgttaacc tcatcaagaa cacctctact gtagctatca tctctggagt
     2221 agacttgatg tttgtgacta agtcttggtc ggctctcaac ggaaactata taccagcctt
     2281 tttaggtgct gctcttctct actttgctct atgcttccct gttgcccagt ttggtcgcaa
     2341 gatggagcaa gccaacaaaa aagcctattc actttaggag gttactatgg aatctatttt
     2401 agaagtttta accccagata acctagtctt tatctttaaa ggatttggct tgaccctcta
     2461 tatttctctg attgccatca tcctctctac tatcatcggt acggtgctag ctgtcacgag
     2521 aaatggcaaa aatcctgtct tacgcattat ttccagtatt tatatcgagt ttgtgcgcaa
     2581 cgttcccaac cttctctgga tttttactat ctttttggtg ttcaaaatga aatccacacc
     2641 agcaggtatt acagccttta ctctctttac atcagcagcc ttggctgaga ttattcgagg
     2701 cggtctcaat gccgtagaca agggacagta cgaagcagga atgtcacaag gcttcacctc
     2761 agcccaaatc ctctactaca tcattctccc acaagccatc cgcaaaatgc taccagccat
     2821 catttctcag tttgttaccg tgattaagga taccagtctc ctctactctg ttatcgccct
     2881 acaagaactc tttggagcca gccaaattct catgggccgt tatttcgaac cagagcaggt
     2941 cttcagtctt tacatcctga ttgccctcat ctacttcagc tttaacctag caatttctag
     3001 cctgtctcat atgctagcca aacgttggca acaagctgca gaataacact cttcgaaaat
     3061 ctcttcaaac cacgtcagct ctatctgcaa cctcaaaaca gtgttttgag caacttgcgg
     3121 ctagcttcct agtttgctct ttgattttca ttgagtataa acagccactc tccagttcaa
     3181 ttggagagtt ttttgatgag agtagatatt tatagacctc aatttgacac catacatttt
     3241 ctatgataaa atgtaacaaa tttatttaca acaaatcgaa atctaaatga aatggaattt
     3301 tctatgaaat cagtcaaagg actactcttt atcatagcta gttttatctt gactcttttg
     3361 acttggatga acacttctcc ccaattcatg attccaggac tagctttaac aagcctatct
     3421 ctgactttta tcctagccac tcgtctccca ctactagaaa gctggtttca cagtttggag
     3481 aaggtctaca ccgtccacaa attcacagcc tttctctcaa tcatcctact aatctttcat
     3541 aactttagta tgggcggttt gtggggctct cgcttagctg ctcagtttgg caatcttgcc
     3601 atctatatct ttgccagcat catccttgtc gcctatttag gcaaatacat ccaatacgaa
     3661 gcttggcgat ggattcaccg cctggtttac ctagcctata ttttaggact ctttcacatc
     3721 tacatgataa tgggcaatcg tctccttaca tttaatcttc taagttttct tgttggtagc
     3781 tatgcccttt taggcttact agctggtttt tatatcattt ttctatatca aaagatttcc
     3841 ttcccctatc tagggaaaat tacccatctc aaacgcttaa atcacgatac tagagaaatt
     3901 caaatccatc ttagcagacc tttcaactat caatcaggac aatttgcctt tctaaagatt
     3961 ttccaagaag gctttgaaag tgctccgcat cccttttcta tctcaggagg tcatggtcaa
     4021 actctttact ttactgttaa aacttcaggc gaccatacca agaatatcta tgataatctt
     4081 caagccggca gcaaagtaac cctagacaga gcttacggac acatgatcat agaagaagga
     4141 cgagaaaatc aggtttggat tgctggaggt attgggatca cccccttcat ctcttacatc
     4201 cgtgaacatc ctattttaga taaacaggtt cacttctact atagcttccg tggagatgaa
     4261 aatgcagtct acctagattt actccgtaac tatgctcaga aaaatcctaa ttttgaactc
     4321 catctaatcg acagtacgaa agacggctat cttaattttg aacaaaaaga agtgcccgaa
     4381 catgcaaccg tctatatgtg tggtcctatt tctatgatga aggcacttgc caaacagatt
     4441 aagaaacaaa atccaaaaac agagcttatt tacgaaggat tcaagttcaa ataagtaatc
     4501 atactctcct gggatttccc aggagagttt tttatagcca aatgaaataa gacaggaacg
     4561 atggaatttt ggcattcaaa tcaattgata agactatttt atagtggagt gaaataggat
     4621 acgaacaaat tgattaggaa aatcaaatga atttatagaa atcttttagc agttatgtta
     4681 tcctattcta gtttcaaaac gctatagaag cagcattgtg ctagtctaga ttcagtttac
     4741 tatactaaaa cgagtagctt gaaatcaaaa aacccaccct cacaggcagg ttttatctgt
     4801 attattcagc tagattatgc tttaccttct gaaccgaata cgtcgatacg ttcttcaacc
     4861 gatgcttgga tagcttttac accgtcagcc aagaatttac gtgggtcgaa gagttttttc
     4921 ttgtcgtatt ctgcttcgtt tgcttcgtag tcacgagcaa atttacgagt tgcgttagcg
     4981 aatgcgattt ggcattctgt gttaacgtta actttggcaa caccaagttt gatagctgct
     5041 tggatttgct catcaggaat acctgatcca ccgtgcaata cgattgggaa tcctggaaga
     5101 gcttctgtca atttttgcaa gtggtcaagg tcaagacctt cccagtttac tgggtaagga
     5161 ccgtggatgt taccgatacc agctgccaag aagtcgatac cagtttcaac cattgcttta
     5221 gcgtcttcga ttggagccaa ttcaccttta ccgatgattc catcttcttc accaccgata
     5281 gtaccaactt cagcttctac tgagatacct ttagcgtgtg ctttttcaac aacttcttta
     5341 gccaatttaa ggttttcttc aactggaagg tgtgaaccgt caaacatgat tgaagtataa
     5401 ccaacttcga tacactcaag tgcatcttcg tagtgaccgt ggtcaaggtg gatagctact
     5461 ggtacagtga tacccattga ttcaacaagg ttagcgatca agttgcgagc aactttgtaa
     5521 ccacccatgt atttagcagc acccattgaa gtttggatca aaactggagc ttttttagct
     5581 tctgctgcgc gcaagatagc ttgagtccac tcaaggttgt ttgtgttaaa tccaccaact
     5641 gcataaccgt tgtcacgggc tgcttggaca aatttttctg ctgaaacgat tgccatttta
     5701 tcaggcctcc tgtatatttt tatgggtcat cccatttaca ttgttcattt tatcactttt
     5761 tgccaaaaaa atctagtttt tcccgcagtt tcgattgatt ttcttctaac tccatctatg
     5821 taaacccttt ctctccctag tcttggacga cttttggaaa atctataaag aaggttaaac
     5881 tattctcctc catctcgaaa cgataagcta atttttcatg ttctaataga ctcttaacca
     5941 caaagagccc cataccagac cccttgacct tgcgactggc attgtcagaa aaagactggg
     6001 ctagtttttc ttgttcctct gagctacagc tattttcgat aaaaagttct ccttctcttt
     6061 ctccaattcg aactaagcca cctggaacag agtgcttaat ggcattgctg atgagattag
     6121 aaagaatcaa cttcataact gatgggttta gataagcctg ctgatgggtc aaactattgt
     6181 ctatctggag ctctctttcc ttggctagca aggcataatc tttgaccaga ttttgcgtca
     6241 tctggaggag gtcaattgtt tccctatcat ctcgcaattc ctgcacagaa gagagggaaa
     6301 gtatctgcag aacatggtga ttgagttcat ccacaatccc caaggcaact cccagatact
     6361 ggtctctatc cttataacga ccgatattct ctctcatatt ttcgattagg attttcaaac
     6421 tagccagcgg tgttttcaat tcatgagaag ctcctcgtag gaattcgacc ttcatcttct
     6481 ccagctggag aatggcttca ttcttttcat gcaagtccgc aataacagtc aagagatgct
     6541 ggtagaggct attgatttgt tccttgagat tacctatctc atccttagaa tccacgcgca
     6601 atcgcacttg ggaatccagg tccatcatcc gacgggtcac ccgcttgatt tccaaaatcg
     6661 gtgcaacaat agtccgagcg tagatgtagg ccaccaaaag ggaaatcaga aaggaggcca
     6721 gcaaggtata gggaagaaac tggagactga tttgctccgc ttccttttgt aaatccatgg
     6781 aagctagaaa ctggagaatc atagtaccac cgtcttgcgt tttcacctcg cgctcctcaa
     6841 taaagagaga ggttgtctgg cggtctgtgt ccagaggaag actgtccttg acttctaact
     6901 tgtcctcggt catctcacct ttgacggtcc ccttgatatc actagtctgg gaatacaagt
     6961 ctaacacttg ctcgatactc tgcctatctt tcccttctag ggactgggca atggctgttg
     7021 ccttttgacc aatggtttcc tgacgatgac tcagataagt cgaaggaaaa agaaaataaa
     7081 tagctaaatg aaggcagata accagaacac taaatatcga gaaggtatag ataaatatct
     7141 ttgcaaataa acctgttcgt ttcattttcg ctccaattta taaccaacat tgcgcacagt
     7201 gaggatacaa tccaagtcta gctttttccg caattccttg atataaacat caataacacg
     7261 gtcaaaggga acctcatctg tcgctttcca gacggcatcg ataatctgag accgagtcaa
     7321 ggcccggcct tcatttttca ctagatagtc cagaatttcc aactctttgg cattgatagg
     7381 cacttcttga cctgcgaggc ttgcactgta gctttcaaag tccaccttgg tatccttgta
     7441 agaaaagatt cgtcctgtat cgtagtagcg cttgaaaatc gcgtccactc tcacttttaa
     7501 gagggagagg gagaaaggtt tttccagata gccatctgcc aaagaggcaa aggcactcat
     7561 cttgtattcc tcatcctgaa aagctgtcaa catcaagaca ggaacctgac tggttttacg
     7621 aatctcagct agaacttcta agccgttaag cttgggcatc tggatatcca gtaaaaccag
     7681 ggccacctca tagctagaaa attgctccag agcttcctga ccgtccgctg cctcaatagt
     7741 ttcatagcca caatccgtca aataatcact gaccccctca cggatcatct cttcatcttc
     7801 tacaattaaa attttcatac tttaactgct ctctattttt tatttttctt agaataaata
     7861 cctactctat tttctattat agtctcttgc tggcgttttg tctgtaagta actgatcact
     7921 agataaaacg ttgtgaaatt cctttctcat aaattccata gctttagtat attatattta
     7981 agcaataaag tacaaagaaa acaactgaaa gcaatgattt tcaccactgc tttcggattt
     8041 attttgaatt gttaaatagc cattcctatc cactattctt gaatagaaac acaagatgca
     8101 atctttattc cagactcatt ttttcaaatt tattcaccat ccagcaagag ctcttttggt
     8161 tgttttctaa ggagattgct tgaagcaagc gccataacga gaaccactag aaccaaggca
     8221 aggacaaaaa tgatgataaa gtctgatgtc tgaatagaaa tgtctaggct cgacaaggtc
     8281 ttgctaaagc catctacttc tgcaccacca ccaaggttag aggcttgagc cgccttacta
     8341 gcctgtttgg caacacctga agtcacattg gcaaggacag tgtttccaat tgcacgggca
     8401 gtgtaattag ctaggaagta agcagaaact agagcaggga tagcaatcaa gatagattcg
     8461 gtgatgaatt gacccaagat acttgcctgc ttgaggccga tagagaggag aattcccact
     8521 tccttgcgac gggcgttgat ccaaaggctg agcaagaggg caaggaggag aactgagaag
     8581 ctcaagctac cccagaagag gaggttggcc atcttgtaca taccagagat agattgctca
     8641 agagctgggt agttagagga gctcttgacg agtgtgtagc tcttccagtt gataccactg
     8701 atgccattca actctttcat aacatcatcc aagttcttgt ctgctgttac aaagaaggtt
     8761 gcgtccccat aaatggctgt gtcttctgtg tatccataaa gttttgcagc agtgtgaatg
     8821 tctgtaatag ctgtgttttc gtaaagttct tgtgagtagg ttactgctga cttattatga
     8881 ccatcaaaga gtcccttgat tgtcacttca actgtttcct tggctccttt ttcattatct
     8941 gcatcgtaga tattagagtc cagtttaacc ttatccccta ctttccagcc gtgtttggct
     9001 gccaagtcct tgtgcaagag gattttatcc ttgtcgtcgt tggttaagtg ctctccttcg
     9061 actagtttat aagaaccaga gacaaacttg tcttctttag aggagtcatt gacacctgta
     9121 atcatcaagc tacttccaaa acgtttggca cgatcagcag tgagattctt cttggtttct
     9181 ggcgtttcaa tcaggtcata tccagtcaaa tctccgatag cgttgatacg tttgacataa
     9241 gactcaatgg ccttgttttc ggtgattttt ttgatgtctt cacccttgat attcccagca
     9301 ccacgaggcg ttccttggtt gacgcgacga ttgatttgca tggagaagct attggtgata
     9361 tttttaaagg tctcctgaga agccttggca gtagctccct tgattgacaa gccgaccaaa
     9421 ctcaagctcg ccatgaggag aataatcagg aagatgacaa tcgatttgaa aaacttcctt
     9481 gtaacatagg caaatgcgtt gtgtaacata gattcccttt ctagattttg ttttaatcat
     9541 tctattaaag taagctcaaa ttatttacta gtattgcgcg tttcagtcag tttcttatcc
     9601 tttaattcaa gtgtaatatc tgacgcttgt gccacttctt tactgtgagt tacgacaatc
     9661 acacatttac ctgttttctg ggcaagtgat ttgagtagtt cgacaatatc tccagcagtt
     9721 ttaggatcca gatttcctgt tggctcatca gctagaataa ctggagcttc tgagaccaaa
     9781 ctgcgagcaa tggcaacacg ttgctgttga ccacctgata actggagaac attccgcttg
     9841 atctggcttt catccaaacc aagctcaaga agtgtatcct tgcttgcctt tttgttgacc
     9901 aagcggatat tttccagcgg agaaagataa tctatcaagt tataattttg aaagaccagg
     9961 gaaatatggt gcatgcgatg gtaagaatag cccttcttac gaatatcctc tccttgaaaa
    10021 aggatagaac cttcaacagg actatctaga ccagcaagta gggacaagag tgtggatttt
    10081 cctgctcctg actccccaat aatactgtaa aattttccgg gttcaaaatt ataattgatc
    10141 tgatatagga ctgcttcagc agtattctta taacggtagg taacatcttg taattgtaat
    10201 aaagtcatga tttctccttc ttaactaata gatgataaaa tttctttcgg tgattttcta
    10261 aataagaata ggaaacaaag ggctacagat aagcaactaa gcagaactag aaaaacatag
    10321 gattctgcaa aagataagat gctagttgat aaactgcttg ctttggctag tgtatcttgt
    10381 aagcttgcct gatctccact tgctagtaga gtttggagta ggtaagttgt gattgcgttt
    10441 cctgcaacaa atgctggaag caaagctcca agagatacca aaactacctc taaacagaat
    10501 tgtaggaaga tcgagctctt gccttttcca agtgcaagta aaatccccac ttcatagacc
    10561 cgttctctca accagagaga caaaaccaga attaaggctc cagctcctgc tatcaacatc
    10621 ccataaagga agatggtcag gaaggtttgg aaagttgcaa ctgagtcttt gatttgttca
    10681 aaagccttgt tttccttttc gacttggtag ccttgatttt ccaaggccaa gttttctacc
    10741 tgcttcatga gtccgtccat ttccttagga ttttctacat agaagcgtgc tgcactgact
    10801 tgagcttcac tattgcccaa aagggtttgg ctactttcat agtctgtaaa gacttgattt
    10861 tcactgaagt cagaagacaa gcctgtgaat ttctcttgtt ttttaccaga aaagatgccg
    10921 ataatctcaa actctactgt ttgtcctttt ccagattcag actgaccagc atccaagcca
    10981 atcttgtcat gaagcgaaag accgttcttc ttagccaatt cttcgtggat aaggattttc
    11041 ttggaatccc ctttttgaag gtgtcgccct tcttttagat tgaaagccga actggtaaag
    11101 gttacatcct tggatgaatc ctcaagagcc gttaagctaa ccaagttatt gtctgcagct
    11161 gataaatcat cacgctccac gctctgctcg ccagtcactg cttccttgtc ttttagtttt
    11221 gcgaccgtct caagttcagg agagacattt tccagcccct taatcttgct tacagatgct
    11281 aggtctgaca acttgaatgt ctgaccattc tctatcttct taatagaaaa agatgtattg
    11341 agtgatttat aaagattgct ttctactgtt ttgttggact tcatcagagt caaacaggct
    11401 gaaattccgg ccaataagac caataaaatc agaaataaaa taaaacttct cagtcgcttt
    11461 ctgctgacat aagcccaaga tctttggatt ggattcattt gtcacctcca tatttgtaag
    11521 actattataa aacccaaata tgaaatattt atgaaatacg aaaaaaaaat atcgagtagg
    11581 ggataatctc tagcccctct cacaccacca tacgtgccgt tcggcatacg gc
//
LOCUS       DAVHEW010000064        11570 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVHEW010000064 DAVHEW010000000
VERSION     DAVHEW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.064"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(202..1833)
                     /locus_tag="VVL53_001512"
     CDS             complement(202..1833)
                     /locus_tag="VVL53_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU4183667.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2126..7105
                     /locus_tag="VVL53_001513"
     CDS             2126..7105
                     /locus_tag="VVL53_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU4183668.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKSRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKSAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7195..8391
                     /locus_tag="VVL53_001514"
     CDS             7195..8391
                     /locus_tag="VVL53_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU4183669.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8527..9054
                     /locus_tag="VVL53_001515"
     CDS             8527..9054
                     /locus_tag="VVL53_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU4183670.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9131..9487
                     /gene="glnR"
                     /locus_tag="VVL53_001516"
     CDS             9131..9487
                     /gene="glnR"
                     /locus_tag="VVL53_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU4183671.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9524..10870
                     /gene="glnA"
                     /locus_tag="VVL53_001517"
     CDS             9524..10870
                     /gene="glnA"
                     /locus_tag="VVL53_001517"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU4183672.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10966..11085)
                     /locus_tag="VVL53_001518"
     CDS             complement(10966..11085)
                     /locus_tag="VVL53_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU4183673.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11343..11480
                     /locus_tag="VVL53_001519"
     CDS             11343..11480
                     /locus_tag="VVL53_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183674.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
BASE COUNT         3701 a         2127 c         2509 g         3233 t
ORIGIN      
        1 ctataataaa atgaaataat aacaggacaa atcgttcagg acagtcaaat cgatttctaa
       61 caatgtttta gaaatagagg tgtactattc tagtttcaat atattatata tctgcagaat
      121 actctttcag tttcattcag cctagtcttg cttaggaaaa agtaagaagc catccaatta
      181 tgatggatag cttcttggtt attagatttg ttctgcaaaa accttttcag caaggttcat
      241 agcgtggtct gatacacgag tgtagtgtga gatgatgtcg ataaagttga ctccagcttg
      301 tgttgaacat tcgcctttgt tgaggcgttt gatgtgggtt tttctgagaa cacgttctat
      361 cttattgatt gcttcatgac gttctacaag actgcgtgct ttttcaatat cattgttttc
      421 cacactatcc agagcatctt tgataaagtc actagtttgg cggtaaactt cctctaattc
      481 tttcaaggcg gcatcagaaa attcaacatt tttccgttga agatagtcag tcagattgag
      541 tagagcctcc gtgtggtctc caatccgttc caaatcacgg gaggaatcaa ggatattggt
      601 aagcacttca ctttcttttt ggctgagagc ttcgcttgaa agggcaatga gataacgtgt
      661 taattgctca tcgatggtgt taattgcttc ttcggtttta tgcccttttt cagcaacttt
      721 ttcatccaag tcaatgatgt acttatagga aaggtcaaag gctttagcag cgtagtttcc
      781 taagtgcaag agctctttct tagcatttcc tagagcgata gatggggcct gtttgatgaa
      841 atgttcatca agatataagg gttcgtattt gacaacctcg tcctctccag gaataatctt
      901 ggttacaaag taagccagag ctccgataaa tggaaattgg acaatggtgt tggtaatatt
      961 aaaggttccg tgagcaaagg cgatggtcat ttccggtgct agatttagcg tagcttcaaa
     1021 ccaatgaatc aggacagtaa aaggaactag aaaaataacg cagacgactg ttccgataac
     1081 gttgaaggca acatgagctc ctgctacccg tttagctgca atattagccc ctaaagaggc
     1141 aatgatggct gtaatggttg tcccgatatt gtcaccaaat agaactggca aagctccctg
     1201 tagatcaatt agattgccgg cgtagaggtt ttgtaaaatc ccaatggtag ccgaagaagc
     1261 ttgaattagc aaggtcaagc cagtaccgac aaagacaccc aaaacaggat ttttacttag
     1321 ctcaatcata tagtccttaa agacctgtaa atccttgagt ggagccattg cgccgctcat
     1381 gagattgagg gcaaaaaaga taccaccgac accaaagagg atgcgtccga tattattgac
     1441 tgtccgattt tttgtaaaaa agagacagac ggcaccgata aagagcatag gtagggcata
     1501 gttacctaat ttaaaaccga tgagaaagga tgtgacagtt gtcccaatat tagcacccat
     1561 gacaatcccg atagcctgac gtaaggttaa gagaccggca ctgaccaggc cgactgtgat
     1621 aactgttaca ccagaactag actgaattag agcagtcatc ccaataccaa ccagaactcc
     1681 aaagaaagga ttactagtat atttgtcaat gtaaaaacga aggcgatctc cagcagcttg
     1741 ttgtaaaccg tctcccatgg tcttgatgct atataagaat agccccagac cacctaaaaa
     1801 gtgaaataaa atttcctgcc aattaatgga catttctttt tcctccgaaa aataatcgcg
     1861 gaatatctcc tattctattt taaaggataa aagtaaatct aacaagtgtt aatgtcaagt
     1921 ttgtaaaaga aaattataat tgataagtgt attattttta agagttttta accttatttt
     1981 agatttgtgc aaacagttat tgttttttgt ctaaaaatgt tttaggataa aaatgtgatt
     2041 atcatggttc tagagtttga taaaaaacca cagaatataa agcgctttct tgaaaacaac
     2101 aaaatcaatc ttttaggagg agaaaatgaa gaatccattt tttgaaagac gttgtcgtta
     2161 cagtattcgt aagttatcag taggagcctg ctcgctgatg attggtgctg ttttatttgc
     2221 tggtccagcc ttggctgaag aaactgcagt tcctgaaaat agcggagcta atacagagct
     2281 tgtttcagga gagagtgagc attcgaccaa tgaagctgat aagcagaatg aaggggaaca
     2341 tgctagagaa aacaagctag aaaaggcaga aggagtagcg atagcatctg aaactgcttc
     2401 gccagcaagc aatgaagctg caactactga aactgcagaa gcagctagcg cagctaaacc
     2461 agaggaaaaa gcaagtgagg tggttgcaga aacaccatct gcagaagcaa aacctaagtc
     2521 tgacaaggaa acagaagcaa agcccgaagc aactaaccaa ggggatgagt ctaaaccagc
     2581 agaagaagct aataagactg aaaaagaagt ccagccagat gtccctaaaa atacagaaaa
     2641 aacattaaaa ccaaaggaaa tcaaatttaa ttcttgggaa gaattgttaa aatgggaacc
     2701 aggtgctcgt gaagatgatg ctattaaccg cggatctgtt gtcctcgctt cacgtcggac
     2761 aggtcattta gtcaatgaaa aagctagcaa ggaagcaaaa gttcaagcct tatcaaacac
     2821 taattctaaa gcaaaagacc atgcttctgt tggtggagaa gagttcaagg cctatgcttt
     2881 tgactattgg caatatctag attcaatggt cttctgggaa ggtctcgtac caactcctga
     2941 cgttattgat gcaggtcacc gtaacggggt tcctgtatac ggtacactct tcttcaactg
     3001 gtctaatagt attgcagatc aagaaagatt tgctgaagct ttgaagcaag acgcagatgg
     3061 tagcttccca attgcccgta aattggtaga catggccaag tattatggct atgatggcta
     3121 tttcatcaac caagaaacaa ctggagattt ggttaaacct cttggagaaa agatgcgcca
     3181 gtttatgctc tatagcaagg aatatgctgc taaggtaaac catccaatca agtattcttg
     3241 gtacgatgcc atgacctata actatggacg ttaccatcaa gatggtttgg gagaatacaa
     3301 ctaccaattc atgcaaccag aaggagataa ggttccggca gataacttct ttgctaactt
     3361 taactgggat aaggctaaaa atgattacac tattgcaact gccaactgga ttggtcgtaa
     3421 tccttatgat gtatttgcag gtttggaatt gcaacagggt ggttcctaca agacaaaggt
     3481 taagtggaat gacattttag acgaaaatgg gaaattgcgc ctttctcttg gtttatttgc
     3541 cccagatacc attacaagtt taggaaaaac tggtgaagat tatcataaaa atgaagatat
     3601 cttctttaca ggttatcaag gagaccctac tggccaaaaa ccaggtgaca aagattggta
     3661 tggtattgct aacctagttg cggaccgtac gccagcggta ggtaatactt ttactacttc
     3721 ttttaataca ggtcatggta aaaaatggtt cgtagatggt aaggtttcta aggattctga
     3781 gtggaattat cgttcagtat caggtgttct tccaacatgg cgctggtggc agacttcaac
     3841 aggggaaaaa cttcgtgcag aatatgattt tacagatgcc tataatggcg gaaattccct
     3901 taaattctct ggtgatgtag ccggtaagac agatcaggat gtgagacttt attctactaa
     3961 gttagaagta actgagaaga ccaaacttcg tgttgcccac aagggaggaa aaggttctaa
     4021 agtttatatg gcattctcta caactccaga ctacaaattc gatgatgcag atgcatggaa
     4081 agagctaacc ctttctgaca actggacaaa tgaagaattt gatctcagct cactagcagg
     4141 taaaaccatc tatgcagtca aactattttt cgagcatgaa ggtgctgtaa aagattatca
     4201 gttcaacctc ggacaattaa ctatctcgga caatcaccaa gagccacaat cgccgacaag
     4261 cttttctgta gtgaaacaat ctcttaaaaa tgcccaagaa gcggaagcag ttgtgcaatt
     4321 taaaggcaac aaggatgcag atttctatga agtttatgaa aaagatggag acagctggaa
     4381 attactaact ggctcatctt ctacaactat ttatctacca aaagttagcc gctcagcaag
     4441 tgctcagggt acaactcaag aactgaaggt tgtagcagtc ggtaaaaatg gagttcgttc
     4501 agaagctgca accacaacct ttgattgggg tatgactgta aaagatacca gcctaccaaa
     4561 accactagct gaaaatatcg ttccaggtgc aacagttatt gatagtactt tccctaagac
     4621 tgaaggtgga gaaggtattg aaggtatgtt gaacggtacc attactagct tgtcagataa
     4681 atggtcttca gctcagttga gtggtagtgt ggatattcgt ttgaccaagt cacgtaccgt
     4741 tgttagatgg gtcatggatc atgcaggagc tggtggtgag tctgttaacg atggcttgat
     4801 gaacactaaa gactttgacc tttattataa agatgcagat ggtgagtgga agctagctaa
     4861 ggaagtccgt ggtaacaaag cacacgtgac agatatcact cttgataaac caatcactgc
     4921 tcaggactgg cgcttgaatg ttgtcacttc tgacaatgga actccatgga aggctattcg
     4981 tatctataac tggaaaatgt atgaaaagct tgatactgag agtgtcaata ttccgatggc
     5041 caaggctgca gcccgttctc taggcaataa caaggtacaa gttggctttg cagatgtacc
     5101 ggctggagca actattaccg tttatgataa tccaaattct caaactccgc tcgcaacctt
     5161 gaagagcgaa gttggaggag acctagcaag tgcaccattg gatttgacaa atcaatctgg
     5221 tcttctttat tatcgtaccc agttgccagg caaggaaatt agtaatgtcc tagcagtttc
     5281 cgttccaaaa gatgacagaa gaatcaagtc agtcagccta gaaacaggac ctaagaaaac
     5341 aagctacgcc gaaggggagg atttggacct tagaggtggt gttcttcgag ttcagtatga
     5401 aggaggaact gaggacgaac tcattcgcct aactcacgca ggtgtatcag tatcaggttt
     5461 tgatacgcat cataagggag aacagaatct tactctccaa tatttgggac aaccagtaaa
     5521 tgctaatttg tcagtgactg tcactggcca agatgaagca agtccgaaaa ctattttggg
     5581 aattgaagta agtcaggaac cgaaaaaaga ttacctagtt ggtgatagct tagacttgtc
     5641 tgaaggacgc tttgcagtgg cttatagcaa tgacaccatg gaagaacatt cctttactga
     5701 tgagggagtt gaaatttctg gttacgatgc tcaaaagact ggtcgtcaaa ccttgacgct
     5761 tcgttaccaa ggtcatgaag tcaactttga tgttttggta tctccaaaag cagcattgaa
     5821 cgatgagtac ctcaaacaaa aattagcaga agttgaagct gctaagaaca aggtggtcta
     5881 taactttgct tcaccagaag taaaagaagc cttcttgaaa gcaattgaag cggccgaaca
     5941 agtgttgaaa gaccatgaaa ctagcaccca agatcaagtc aatgaccgac ttaataaatt
     6001 gacagaagct cataaagctc tgaatggtca agagaaattt acggaagaaa agacagagct
     6061 tgatcgctta acaggtgagg ctcaagaact cttggctgcc aaaccaaacc atccttcagg
     6121 ttctgcccta gctccgcttc ttgagaaaaa caaggccttg gttgaaaaag tagatttgag
     6181 tccagaagag cttgcaacag cgaaacagag tctaaaagat ctggttgctt tattgaaaga
     6241 agacaagtca gcagtctttt ctgatagtaa aacaggtgtt gaagtacact tctcaaataa
     6301 agagaagact gtcatcaagg gtttgaaagt agagcgtgtt caagcaagtg ctgaagagaa
     6361 gaaatacttt gctggagaag atgctcatgt ctttgaaata gaaggtttgg atgaaaaagg
     6421 tcaagatgtt gatctctctt atgcttctat tgtgaaaatc ccaattgaaa aagataagaa
     6481 agttaagaaa gtatttttct tacctgaagg caaagaggca gtagaattgg cttttgaaca
     6541 aacggatagt catgttatct ttacagcacc tcactttact cattatgcct ttgtttatga
     6601 atctgctgaa aaaccacaac ctgctaaacc agcaccacaa aacacagtcc ttccaaaacc
     6661 tacttatcaa ccggcttctg atcaacaaaa ggctcctaaa ttggaagttc aagaggaaaa
     6721 ggttgccttt catcgtcaag agcatgaaaa tgctgagatg ctagttgggg aacaacgagt
     6781 catcatacag ggacgagatg gactgttaag acatgtcttt gaagttgatg aaaacggtca
     6841 gcgtcgtctt cgttcaacag aagtcatcca agaagcgatt ccagaaattg ttgaaattgg
     6901 aacaaaagta aaaacagtac cagcagtagt agctacacag gaaaaaccag ctcaaaatac
     6961 agcagttaaa tcagaagaag caagcaaaca attgccaaat acaggaacag ctgatgctaa
     7021 tgaagcccta atagcaggct tagccagcct tggtcttgct agtttagcct tgaccttgag
     7081 acggaaaaga gaagataaag attaaatatc gaaaaatctt gtgaaatctt tccttatatt
     7141 tccaaagtgt gatataatag tttcgaataa aataaataaa ggagtttttg taacatggca
     7201 aaacttactg ttaaagacgt tgacttgaaa ggtaaaaaag tcctcgttcg tgttgacttc
     7261 aacgtaccat tgaaagatgg cgtaatcact aacgataacc gtatcacagc agctcttcca
     7321 actattaagt acatcatcga acaaggtgga cgtgcaattc ttttctctca ccttggacgt
     7381 gtgaaagaag aagctgataa agctggtaaa tcacttgctc ctgtagcagc tgacttggca
     7441 gcaaaacttg gtcaagatgt tgttttccca ggtgtcactc gtggtgctga attggaagcg
     7501 gcaatcaacg ctcttgaaga tggacaagtt ctcttggttg aaaacactcg ttacgaagat
     7561 gttgacggca agaaagaatc taaaaacgat cctgaacttg gtaaatactg ggcatcactt
     7621 ggagatggta tcttcgtaaa cgatgcattc ggtacagctc accgtgcaca cgcatctaac
     7681 gttggtatct cagcaaacgt tgaaaaagca gttgctggtt tccttcttga aaacgaaatt
     7741 gcctacatcc aagaagcagt tgaaactcca gaacgtccat tcgtggctat ccttggtggt
     7801 tcaaaagttt cagacaagat cggtgttatc gaaaacttgc ttgaaaaagc tgataaagtc
     7861 cttatcggtg gtgggatgac ttacacattc tacaaagcac aaggtatcga aatcggtaac
     7921 tcacttgtag aagaagacaa attggatgtt gcgaaagctc ttcttgaaaa agcaaatggt
     7981 aaattgatct tgccagttga ctcaaaagaa gctaacgcat ttgctggtta cactgaagtg
     8041 cgtgacactg aaggtgaagc agtttctgaa ggcttccttg gtcttgacat cggtccaaaa
     8101 tctatcgcca aatttgacga agctttgact ggtgccaaaa cagttgtatg gaacggacct
     8161 atgggtgtat ttgaaaaccc agatttccaa gctggtacaa tcggtgtgat ggacgctatc
     8221 gtgaaacaac caggagttaa atcaatcatc ggtggtggtg actcagctgc cgcagcgatt
     8281 aaccttggcc gtgcagacaa gttctcatgg attagtacgg gtggtggagc atcaatggaa
     8341 cttcttgaag gtaaggttct tccaggactt gcagccttga cagaaaaata agattttata
     8401 aacaaatcaa agaagagagg gatgagagtt cctcttttct tttgcttaaa ataaaaacgc
     8461 ttcctctcaa ctattactca taaaaatcac cgatttatga taaaatggaa atagaaagtt
     8521 gagattatga gttattttaa aaaatataaa ttcgataaat cccagttcaa acttggtatg
     8581 cgaaccttta aaacaggtat tgctgttttt ctagttctct tgatttttgg cttttttggc
     8641 tggaaaggtc ttcaaattgg tgctttgaca gccgttttta gcctgaggga gagttttgat
     8701 gagagtgttc attttgggac ttcgcgtatt ctaggaaata gtatcggtgg actctatgcc
     8761 ttggtcttct tcttattaaa tacctttttc catgaagcct tttgggtgac cttggtagtt
     8821 gttccaatct gcaccatgtt aaccattatg acaaatgtag ccatgaataa caaagcaggg
     8881 gttattggtg gggtagcagc tatgttaatc attaccctat caattccaag tggtgagaca
     8941 atttcgtacg tgtttgtgcg tgtatcagaa acgtttatgg gagtttttgt cgcaattatc
     9001 gtaaattacg atattgatcg tattcgtctc tttttagaga aaaaagaaaa ataatgttac
     9061 attttataac attatcaatt gacgtttgtc tttttttaga ctataataga cagaaagaag
     9121 gaaattgtaa atgaaggaaa aagaatttcg ccgaaatatg gctgtttttc ctatcggcag
     9181 tgttatgaag ttgaccgatc tatcggcgcg tcagattcgt tattatgaag atcaagagtt
     9241 gatcaagccc gatcgaaacg aaggaaatcg tcgcatgtat tccttgaatg acatggatcg
     9301 tctgcttgaa atcaaagatt atatctctga aggttataat atcgctgcca ttaagaaaaa
     9361 atatgctgaa cgtgaagcga aatccaagaa agcggttagt cagactgaag tacgtcgtgc
     9421 acttcacaat gaactcctcc aacaggggcg ttttgcttca gtacagtcac cttttggtcg
     9481 cggttaggca accgcaagta gtcatagatt aaggagaaaa cttatgccaa tcacagctgc
     9541 agatattcgt cgtgaagtca aggaaaaaaa tgttaccttt attcgtctta tgttctcaga
     9601 tattttggga accatgaaaa acgtcgaaat tcctgctaca gatgaacagt tagataaggt
     9661 cttgtcgaac aaggttatgt ttgatggatc ttctattgaa ggttttgtac gtatcaatga
     9721 gtcggatatg tacttgtacc cggacttgga tacatggaca gtcttccctt ggggagatga
     9781 aaatggaagt gttgcaggtc tgatctgtga tgtctataca acagaaggtg aaccatttgc
     9841 gggtgaccct cgtggtaatt tgaaacgagc tcttcgtcac atggaagaag ttggattcaa
     9901 atccttcaac cttggtccag agccagaatt cttcctattt aagttggatg aaaatgggga
     9961 cccaacactt gaagtgaatg acaagggtgg ctactttgat ttggcaccta ctgaccttgc
    10021 ggacaacaca cgtcgtgaga ttgtgaatgt cttgaccaaa atgggatttg aagtagaagc
    10081 gagtcaccac gaggttgcgg ttggacagca tgagattgac tttaagtacg atgaagttct
    10141 ccgtgcttgt gataagattc aaatctttaa gcttgttgtt aaaaccattg ctcgcaaaca
    10201 cggactttac gcaacattta tggcgaagcc aaaatttggt attgctggat caggtatgca
    10261 ctgtaatatg tccttgtttg atgcagaagg aaataacgcc ttctttgatc caaatgatcc
    10321 aaaaggaatg cagttgtcag aaacagctta ccatttccta ggcggtttga tcaagcatgc
    10381 ttacaactat actgccatca tgaacccaac agttaactca tacaaacgtt tggttccagg
    10441 ttatgaagcg cctgtttaca ttgcttgggc tggtcgtaac cgttcgccac ttgtgcgcgt
    10501 acctgcttca cgtggtatgg gaactcgtct tgagttgcgt tcagtggatc caatggcgaa
    10561 cccttacgtt gctatggctg ttcttttgga agttggtttg tatggtattg aaaataaaat
    10621 cgaagcacca gctcctatcg aagaaaatat ctacatcatg acagcagaag agcgcaagga
    10681 agctggtatt acagaccttc catcaactct tcacaacgct ttgaaagctt tgacagaaga
    10741 tgaagtggtt aaagctgctc tcggagatca catctatact agcttccttg aagccaaacg
    10801 aatcgaatgg gcaagttatg caaccttcgt ttcacaatgg gaaattgata attatttaga
    10861 cctttactaa tactaatata gaagaaagat tgcctgtggg taaacagtca cacagcagtc
    10921 taagccatca aatacctata acccttaaaa tagcgatttc ggtcgctatt tatttttcga
    10981 aaaatcctct tgactctttt gcgaattaga tttatcatca gacgtgtaga agaaaattaa
    11041 ggagtcacta tggaaaaatt caatttcaaa aacaatatcg ggcaagaaaa caagttactt
    11101 caaatcgaaa tctacaagtt tactaacttt tgcaagcttc aaaattacac atctgtaaat
    11161 attttttcga aagatatatt tgaagccata gtaaattgag actagaatag tacactacat
    11221 ctgctaaaaa tttctagaaa ctaatttaac tttcctaatc gatttgttta catcttattt
    11281 caatctacta tattatctca ataaatgatg atggcgactg ttttgtttgt gcttgtcgat
    11341 ttttgaatcc tgatcgagcg gaagaatatt tctgccgagg ttgtcaaggg gaaaatcctg
    11401 aagatattga attttatgat gaacaattac aggcagaaaa ggtggaggct ttgaatattc
    11461 gattggaagt aaaaaattga attatctaca atctcatcaa caataaacct atttcaaccc
    11521 cattatctat aggcgtattt ttacgccttt ttatatactt gaagcacata
//
LOCUS       DAVHEW010000065        11431 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVHEW010000065 DAVHEW010000000
VERSION     DAVHEW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.065"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            4..840
                     /locus_tag="VVL53_001520"
     CDS             4..840
                     /locus_tag="VVL53_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183675.1"
                     /translation="MKRKELRLSQQTLAEGICEQSQISKIERGHFIPSADLLFKLSQR
                     LEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERSTFLTLEDRT
                     YLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFYSLVGREQEY
                     EANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEALETIELCKQ
                     RQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMKIENYLKELD
                     TV"
     gene            1223..1843
                     /locus_tag="VVL53_001521"
     CDS             1223..1843
                     /locus_tag="VVL53_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183676.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1929..2372
                     /gene="tsaE"
                     /locus_tag="VVL53_001522"
     CDS             1929..2372
                     /gene="tsaE"
                     /locus_tag="VVL53_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU4183677.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2362..2880
                     /locus_tag="VVL53_001523"
     CDS             2362..2880
                     /locus_tag="VVL53_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183678.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2888..3904
                     /locus_tag="VVL53_001524"
     CDS             2888..3904
                     /locus_tag="VVL53_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU4183679.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNLLRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3989..5245
                     /locus_tag="VVL53_001525"
     CDS             3989..5245
                     /locus_tag="VVL53_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU4183680.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5300..6466
                     /gene="recA"
                     /locus_tag="VVL53_001526"
     CDS             5300..6466
                     /gene="recA"
                     /locus_tag="VVL53_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU4183681.1"
                     /translation="MAKKPKKLEEISKKFGSEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6772..8142
                     /locus_tag="VVL53_001527"
     CDS             6772..8142
                     /locus_tag="VVL53_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU4183682.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8518..9474
                     /gene="lytA"
                     /locus_tag="VVL53_001528"
     CDS             8518..9474
                     /gene="lytA"
                     /locus_tag="VVL53_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU4183683.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            9832..9993
                     /locus_tag="VVL53_001529"
     CDS             9832..9993
                     /locus_tag="VVL53_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183684.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            9980..10186
                     /locus_tag="VVL53_001530"
     CDS             9980..10186
                     /locus_tag="VVL53_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183685.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            10232..10591
                     /locus_tag="VVL53_001531"
     CDS             10232..10591
                     /locus_tag="VVL53_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183686.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            10720..11145
                     /locus_tag="VVL53_001532"
     CDS             10720..11145
                     /locus_tag="VVL53_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183687.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
BASE COUNT         3590 a         1861 c         2515 g         3465 t
ORIGIN      
        1 agattgaaaa gaaaagagtt gagactctcc caacaaactc ttgcagaagg aatttgtgaa
       61 caaagccaga ttagtaaaat tgaaagaggt cattttattc catccgcaga cttgttattc
      121 aaactctctc aacgacttga agtccctttg gattactttt tcaatgaaca aatcgaagtt
      181 aagtctaacc tctctaattt caaacatcta tctgctcgcc tattagatga cagaaattat
      241 gacgatttag aatatcttta taaaatagaa gtagaacgaa gcacttttct aacattagaa
      301 gaccgaactt accttgaatg gattaaatct ataattgact tctatcaata tgacagtaag
      361 tgtgaggcaa tttcgtcatt ggaaaatata ttattaaaag tctcttcaaa tactctgatt
      421 tatttaaagg cgttgaatac tctatctaat ttctattcct tagtgggtcg tgaacaagaa
      481 tatgaggcaa actactctca tttaatagag ttgtatcaga caaaaaattt ggaccatcaa
      541 gagtttttat ttggctacat cagagttcgt tacaactacg ctcactacct agtatcaaag
      601 gaaaaatata atgaagccat ccaagaagct cttgaaacga ttgaactctg taaacaaaga
      661 cagacaagct accaattggc tcctctactt attcttgtag gaaatgctgg agccaaattt
      721 ctagacagag aacaagtcaa aaattattat atagaagcaa gagagttatg taagatttat
      781 aacaatcctt taatgttgat gaagatagaa aattatttga aggaactaga tactgtttag
      841 ttaatcttga cattatagtt tttctgaaac gttaaataac ctgtaaggct gatagttaga
      901 ttaatactat cagccttata attatgtgac tctgagtccg tctcgctccg tgaggtgcga
      961 gacaaaacaa ccacccgttt gacggatttt catgatttca aatttatatt cttcgaaaat
     1021 ctcttcaaat cacgtcagct ctatctgcaa tctcaaaaca ctgttttgag caacctgtgg
     1081 ctagctacct agtttgatct ttgattttca ttgagtatta gtctctagtg tttgtttagc
     1141 gtcttaaaga gacatttgag aagattagaa acagttctct cttttctcct agtataatga
     1201 ttggtaggaa gggagagaga agatgaaatc aatgagaatc ttatttttgt tagctttaat
     1261 tcaaatcagt ttgagtagct gtttcctatg gaaggaatgc atcttgtcct ttaaacaaag
     1321 tacagctttt ttcatcggaa gcatggtttt cgtttcagga atctgtgctg gagtaaatta
     1381 tctttatacc cgtaagcaag aagtccatag tgtcctagcc agtaagaagt cggtgaagct
     1441 tttttacagt atgttactct taattaattt gttaggagct gttcttgttt tgtcagataa
     1501 cttgttcatc aaaaatacgc tgcagcaaga attagttgac tttttattgc catccttctt
     1561 tttcctattt gggctagatt tgctgatttt tttacccttg aaaaaatacg tgcgcgattt
     1621 tcttgctatg ctggacagaa aaaagacagt gttggtgact attttagcaa cacttctttt
     1681 cttaagaaat ccaatgacca ttgtctcact tctgatttat attggactgg gcttgttttt
     1741 tgcagcctat cttgtcccaa attcggttaa gaaggaagtt tccttttatg gtcatatttt
     1801 ccgagatctt gtattggtca ttgttacgct cattttcttt tagagaaagc ctgtttttta
     1861 tggatgttat gatgataagt ttagtaggaa aatagacaca aaagaaaagt ttttggtata
     1921 ataagagtat gtacacaaaa aatgaagaag agttgcaagc cttaggggag cgtttgggcc
     1981 atctattagc aaagaatgat gttttaatct taactggaga actgggtgca ggtaaaacga
     2041 cctttactaa aggacttgca aaaggattac agatttctca aatgattaaa agtcccacct
     2101 atactatcgt gagagagtat gaaggtcgac ttccacttta tcacctagat gtttatcgta
     2161 ttgaaggaga tgctgattct atcgacttgg atgagtttat ctttggtggc ggcgtgactg
     2221 ttattgagtg gggaaatctc ttaggagatg ccttgccaga tgcttattta gaattggaaa
     2281 ttctaaaaga agcagatgga cgccgtttaa attttcaggc aaagggtttg cgtgctgaga
     2341 aattgttaga ggagcttcaa tatggagtat gaattgctca ttagggaagc agagcccaaa
     2401 gatgcagctg aattagtggc ctttttaaat cgtgtgagtt tggagacaga ctttaccagc
     2461 ctagacggag atggtattct cttgactagt gaggagatgg aaatattcct caacaagcaa
     2521 gctagttcgg acaatcagat aaccttactt gcattcttaa atggcaaaat tgctggtatt
     2581 gtaaatatta cagctgatca gcgtaagaga gtccgtcata ttggagatct cttcattgtg
     2641 attggaaaaa gatattggaa taatggcttg ggaagtttgt tgctagaaga agcgatagag
     2701 tgggcacaag caagtggcat tctgcgtcgt ctccaactga ctgtccaaac tcgtaatcaa
     2761 gcagcagtcc atctttatca aaagcatggc tttgtcattg aaggtagcca agagcgtggg
     2821 gcatatatag aagaagggaa atttatcgat gtttacttga tgggtaaact gataggttag
     2881 tagaaatatg gttaaaaaaa ttattggaat ggtgctagct ttactttctg taactgtagt
     2941 aggagtaggt gtttttgctt atactattta tcaacaaggg acagaaacct tagctaaaac
     3001 ctataaaaaa atcggtgaag aaaccaaggt tattgaagcg actgaacctc taaccattct
     3061 gttaatggga gtggacaccg gaaatgttga acgaactgaa acttgggtcg gtagaagtga
     3121 tagcatgatc ttgatgacag tgaatcctaa aacgaaaaaa acaacaatga tgagtttaga
     3181 gcgggatatt ctgacgcgca ttgaatcagg gaatggtcag gctcatgaag cgaaactgaa
     3241 ctcagcatat gcagatggtg gagcagagct tgctatagaa accattcaaa aaatgatgaa
     3301 tatccatatt gatcgctatg tgatggtcaa tatgagagga ttgcaaaaac tagtggatgc
     3361 agtaggaggt attacagtca ataatatcct aggtttccca atttctatca gtgaccaaga
     3421 agaatttaat actatttcta tcggtgttgg ggagcaacat attgggggag aagaagccct
     3481 agtctatgca cgaatgcgtt accaagatcc tgagggggat tatggtcgtc aaaaacgtca
     3541 acgtgaagtt attcaaaaag tcatggaaaa agctctcagt ttaaatagca ttggtcatta
     3601 tcaagagatt ctaaaagctt tgagtgacaa tatgcagacc aatattgatt tgtctgcaaa
     3661 aagtatccct aacttgctag gctataaaga ttcatttaaa accattgaaa ctcagcagtt
     3721 gcagggtgaa ggagagatac ttcaaggtgt ttcttaccag attgtttcga gagcacatat
     3781 gttggaaatg caaaatctac tccgacgttc tttgggacaa gaagaagtta ctcagcttga
     3841 aaccaatgcg gttttatttg aagatttatt tggcagagca cctgttggtg atgaagataa
     3901 ttaacttttg atacaaataa aaaaatcaat cgtaggaaaa ttcctgcgat tttttcaaaa
     3961 aaatacgaat agataggtag gaggaaacat gaaagcagaa atcattgctg ttggaacaga
     4021 gattttgaca gggcagattg tcaacaccaa tgcccagttt ttgtcggaaa aactagctga
     4081 gattggggta gatgtatatt ttcagacggc tgtaggagac aatgaagttc gtctcttgtc
     4141 tttgcttgag attgccagtc aacgtagcag tctggtgatt ttgacaggcg gtttggggcc
     4201 aactgaggac gacctaacca aacaaaccct agctaaattt ttagggaaag cattagtctt
     4261 tgatcctcag gctcaggaga agttggatat cttttttacc ctgcgaccag actatgcccg
     4321 aacaccgaat aacgaaagac aagctcaaat tgtagaagga gcgattccac tgccaaacga
     4381 aacaggactg gctgttggag gaaaattaga agtagacgga gtgacctatg tcgtccttcc
     4441 aggtccgcca agtgaattga aacccatggt cttaaaccaa cttctaccca agttgatgac
     4501 agggagcaag ctgtattccc gagttcttcg tttctttggg attggcgaga gccagttggt
     4561 tacgattttg gctgatttaa ttgataatca gatcgatcct accttggccc cttatgccaa
     4621 gacaggagaa gtcactctac gtctgtcaac aaaggctagc agtcaagaag aggcgaatca
     4681 agcgctggat atcttggaaa atcaaatctt ggactgccag actttcgaag gaatttcttt
     4741 acgagacttt tgctatggtt atggggaaga aactagttta gccagcattg tggtagaaga
     4801 actgaaaagg caagggaaaa ccatcgcggc tgcagagagt ttgacggcag gtcttttcca
     4861 agctaccgtg gcgaattttt ctggagtttc aagtatattt aagggtggtt ttgtgaccta
     4921 tagcttggag gaaaaatcaa ggatgttgga tattcctgcc aagaatttgg aagaacatgg
     4981 tgtggtgtct gaatttacag ctcagaagat ggctgagcag gcacgaagca agacccagtc
     5041 tgattttgga attagtttga ctggagtggc aggaccagat agcctagaag gacacccagt
     5101 tgggacagtc ttcataggct tggcgcaaga tcaaggaact gaggttatca aggttaatat
     5161 tggaggcaga agccgagcag atgtacgtca cattgcggtt atgcatgcct ttaacctagt
     5221 tcgcaaggct ttattaagtg actaactttt gatataatag tagataggtc tgaggatcat
     5281 tagaatgtag gagaatagaa tggcgaaaaa accaaaaaaa ttagaagaaa tttcaaaaaa
     5341 atttgggtca gaacgtgaaa aggccttgaa tgacgctctt aaattgattg agaaagactt
     5401 tggtaaagga tcaatcatgc gtttgggtga acgtgcggag caaaaggtgc aagtgatgag
     5461 ctcaggttct ttagctcttg acattgccct tggctcaggt ggttatccta agggacgtat
     5521 catcgaaatc tatggcccag agtcatctgg taagacaacg gttgcccttc atgcagttgc
     5581 acaagcgcaa aaagaaggtg ggattgctgc ctttatcgat gcggaacatg cccttgatcc
     5641 agcttatgct gcggcccttg gtgtcaatat tgacgaattg ctcttgtctc aaccagactc
     5701 aggagagcaa ggtcttgaga ttgcgggaaa attgattgac tcaggtgcag ttgatcttgt
     5761 cgtagtcgac tcagttgctg cccttgttcc tcgtgcggaa attgatggag atatcggaga
     5821 tagccatgtt ggtttgcagg ctcgtatgat gagccaggcc atgcgtaaac ttggcgcctc
     5881 tatcaataaa accaaaacaa ttgccatttt tatcaaccaa ttgcgtgaaa aagttggagt
     5941 gatgtttgga aatccagaaa caacaccggg cggacgtgct ttgaaattct atgcttcagt
     6001 ccgcttggat gttcgtggta atacacaaat taagggaact ggtgaccaaa aagaaaccaa
     6061 tgtcggtaaa gaaactaaga ttaaggttgt aaaaaataag gtagctccac cgtttaagga
     6121 agccgtagtt gaaattatgt acggagaagg aatttctaag actggtgagc ttttgaagat
     6181 tgcaagcgat ttggatatta tcaaaaaagc aggggcttgg tattcttaca aagatgaaaa
     6241 aattgggcaa ggttctgaga atgctaagaa atacttggca gagcacccag aaatctttga
     6301 tgaaattgat aagcaagtcc gttctaaatt tggcttgatt gatggagaag aagtttcaga
     6361 acaagatact gaaaacaaaa aagatgagcc aaagaaagaa gaagcagtga atgaagaagt
     6421 tccgcttgac ttaggcgatg aacttgaaat cgaaattgaa gaataagctg ttaaagcagt
     6481 ggagaaatcc gctacttttt cgatttttga ttcaagtttt tagattatat atagtagctt
     6541 gaaataagat atgaacaact ctattaggaa agtcaaatta atttctagaa atgttttagc
     6601 agctacagcg tactattcca aactcaacca actataatag atcgaaacta gaatagtaca
     6661 tatctacttc taaaacattg ttaaaaatcg atttgacttt ccttatttca ttccgctata
     6721 tatagtttgc tgtttcttgt cgctcctctg gaaagctgat ataatagctt tatgaataaa
     6781 aaacgaacag tggacctgat acatggtccg attcttccct cgctcttaag cttcaccttt
     6841 ccaattttgc tatcaaatat ttttcaacag ctctataaca ctgctgatgt cttgattgtt
     6901 ggacgatttc ttggtcaaga atccttggct gcagtaggag cgacgacagc gatttttgac
     6961 ctgattgtag gttttacact tggtgttggc aatggcatgg ggattgtcat tgctcgttat
     7021 tatggggctc ggaatttcac taaaatcaag gaagcagtag cagccacctg gattttaggt
     7081 gctcttttga gcattctagt tatgttgctg ggctttcttg gcttgtatcc tctcttgcaa
     7141 tacttagata ctcctgcaga aattcttcct caatcttatc aatatatttc tatgattgtg
     7201 acctgtgtag gtgtcagctt tgcttataat ctttttgcag gcttgttgcg gtctattggt
     7261 gacagtctag cagccctggg atttctgatt ttctctgcct tggttaatgt ggttctggat
     7321 ctctatttta ttacgcaatt gcatctggga gttcaatccg caggacttgc taccattatt
     7381 tcgcaaggtt tatcagcggt tctctgcttt tattatattc gtaaaagtgt gccagaactc
     7441 ttgccacagt ttaaacattt caaatgggac aaaagcttgt acgcggatct cttggagcaa
     7501 ggtttggcta tgggcttgat gagttcaatt gtatctatcg gcagtgtgat tttacagttt
     7561 tctgttaata catttggtgc agtgattatt agtgcccaga cggcagctcg acgcattatg
     7621 acctttgccc ttcttcctat gaccgctatt tctgcatcaa tgacgacctt tgcttctcag
     7681 aatctaggag ctaagcgacc tgaccgtatt gttcaaggtc ttcgaatcgg cagtcgttta
     7741 agtatatcct gggcagtttt tgtttgtatt ttcctctttt ttgccagtcc agctttggtt
     7801 tccttcttgg ctagttcgac agatggttac ttgatagaaa atggaagtct ctatctgcaa
     7861 atcagttcaa ccttttatcc cattttgagc ctcttgttga tttatcgcaa ttgcttgcag
     7921 ggcttggggc aaaagatcct tcctctagtt tctagcttta ttgaactaat cggaaaaatc
     7981 gtttttgtgg ttttgattat tccttgggca ggatataagg gtgttatcct ttgtgaacct
     8041 cttatctggg ttgccatgac agttcaactg tacttctcat tattccgtca tcccttgata
     8101 aaagaaggca aggcaatctt ggcaaccaaa gtgcaatcct agttggattt actgaataaa
     8161 atccatttcc tctagtgaaa atcgaaaaaa cttgtgttct cttctttagt ttggtgttga
     8221 aaatagttta acagactttt gacttctttt atatgatata ataaagtata gtatttatga
     8281 aaaggacata tagagactgt aaaaatatac ttttgaaaat ctttttagtc tggggtgtta
     8341 ttgtagatag aatgcagacc ttgtcagtcc tatttacagt gtcaaaatag tgcgttttga
     8401 agttctatct acaagcctaa tcgtgactaa gattgtcttc tttgtaaggt agaaataaag
     8461 gagtttctgg ttctggattg taaaaaatga gttgttttaa ttgataagga gtagaatatg
     8521 gaaattaatg tgagtaaatt aagaacagat ttgcctcaag tcggcgtgca accatatagg
     8581 caagtacacg cacactcaac tgggaatccg cattcaaccg tacagaatga agcggattat
     8641 cactggcgga aagacccaga attaggtttt ttctcgcaca ttgttgggaa cggttgcatc
     8701 atgcaggtag gacctgttga taatggtgcc tgggacgttg ggggcggttg gaatgctgag
     8761 acctatgcag cggttgaact gattgaaagc cattcaacca aagaagagtt catgacggac
     8821 taccgccttt atatcgaact cttacgcaat ctagcagatg aagcaggttt gccgaaaacg
     8881 cttgatacag ggagtttagc tggaattaaa acgcacgagt attgcacgaa taaccaacca
     8941 aacaaccact cagaccacgt tgacccttat ccatatcttg ctaaatgggg cattagccgt
     9001 gagcagttta agcatgatat tgagaacggc ttgacgattg aaacaggctg gcagaagaat
     9061 gacactggct actggtacgt acattcagac ggctcttatc caaaagacaa gtttgagaaa
     9121 atcaatggca cttggtacta ctttgacagt tcaggctata tgcttgcaga ccgctggagg
     9181 aagcacacag acggcaactg gtactggttc gacaactcag gcgaaatggc tacaggctgg
     9241 aagaaaatcg ctgataagtg gtactatttc aacgaagaag gtgccatgaa gacaggctgg
     9301 gtcaagtaca aggacacttg gtactactta gacgctaaag aaggcgccat ggtatcaaat
     9361 gcctttatcc agtcagcgga cggaacaggc tggtactacc tcaaaccaga cggaacactg
     9421 gcagacaagc cagaattcac agtagagcca gatggcttga ttacagtaaa ataataatgg
     9481 aatgtctttc aaatcagaac agcgcatatt attaggtctt gaaaaagctt aatagtatgc
     9541 gttttcttgt ggagatattt ccttcaattt tgctactata ttaaacaaaa atcaaaaagc
     9601 aaactagaaa gttatgctca aataaaatct aaatttgaca atgtaaaccg agtcggatag
     9661 ctttaagtac tgttttgagg ttgaagatac gatttttgat aggaactcat caattttaga
     9721 tttttaagca gcatcaataa attgcttcct tgttttgtca taattttttt atttaaaaaa
     9781 ttatgacaag agtgtgctat tctttttatg agaggtgtat gaatatgata aatgtatgtg
     9841 ataaatgtat gtgatgttgg aaaaagaata aaagaactta gaatatcttc aaatcttact
     9901 caagataaga ttgctgagta tttgtctttg aatcaaagca tgattgccaa aatggaaaaa
     9961 ggtgaaagga atatcacgaa tggatttaag taataaagct tcaaatctta gaaaaaagtt
    10021 gggagctgat ggtgaatcgc cgatagatat ttttaaattg gtacaaaaga tagaaaattt
    10081 gacgctggta ttttatggac tcggaaagaa tattagcgga gtctgttata aaggaactca
    10141 gttcagtctc attgcagtca attcagacat gccattagga aggtaaagat tttctttagc
    10201 acatggactg tatcatcttt attatgatga ggtgaagaag agttcagtca gtcttatctt
    10261 gattggtgaa ggagatgaaa ctgaaagaaa agcggatcag tttgcttctt attttttaat
    10321 tttcccatct tcactgtata ggatggttga ggaaatcaga gaaaatgcca atagaactca
    10381 tcttgaagta gaagatatta taaaattggg tcagttttat ggtatcagtc ataaagctat
    10441 gttatataga ttgaggaatg atggatacct tgatgcagaa gaaattaaaa atatggatat
    10501 tagtgttata gagacagctt caagattagg ctatgataca agtttatatc gtcctttgtc
    10561 agaaagtaaa aaagaaatgg cattaggata atatattaat tcaactgaac aacttttaga
    10621 aaataacaga atttcgcaag ggaagtatga ggaactgtta ctagatgctt tcagatatga
    10681 tattgtatat gggctagatg aagagggggg agttgtcgtt tgactagtcg tgtatttatt
    10741 gatgcagatt gtatttcagt atttttatgg gttggcactg aacatctttt agaaaagctc
    10801 tatttgggta aaattgttat tccacaagag gtgtatgatg aaatcaatat acctacaatt
    10861 ccccatttaa aatctaggat agatcagttg gtagctaagg gttcagctga gattgtgagc
    10921 atagacattg gaactgaaga atacgcatta tatagagatt taacaagaaa tcatgatagt
    10981 aacaagatta ttggtaaggg agaaggggca tctatttcct tagcgaaaaa gcataatggg
    11041 atattaggaa gtaataacct aagagatgtt aaatcatatg tagaagaatt ttctttagaa
    11101 tatatgacaa caggagatat actgattgaa gcgtttaaag cgtaatttat tactgaataa
    11161 gagggcaatc atatctggaa taatatgctt aaaaagagaa ggaaaattgg tgcaaattca
    11221 ttttcagact atcttcgtgg aagtattcat caaaatagac aaaaataaat ttggataaat
    11281 cgaactcact attcaggagg catatgagca attcgaaaaa gaaaagtgtc aaattgagcc
    11341 tataggagta gaagtgaaat agtaagtcct gcatagtgga tgagagaaaa gttctccttg
    11401 aagttttcct gaactatcag tcgcatgtca a
//
LOCUS       DAVHEW010000066        11211 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVHEW010000066 DAVHEW010000000
VERSION     DAVHEW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.066"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            206..1213
                     /locus_tag="VVL53_001533"
     CDS             206..1213
                     /locus_tag="VVL53_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU4183688.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            1288..1488
                     /locus_tag="VVL53_001534"
     CDS             1288..1488
                     /locus_tag="VVL53_001534"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU4183689.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            1523..2239
                     /gene="atpB"
                     /locus_tag="VVL53_001535"
     CDS             1523..2239
                     /gene="atpB"
                     /locus_tag="VVL53_001535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU4183690.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            2253..2747
                     /gene="atpF"
                     /locus_tag="VVL53_001536"
     CDS             2253..2747
                     /gene="atpF"
                     /locus_tag="VVL53_001536"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU4183691.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            2747..3283
                     /locus_tag="VVL53_001537"
     CDS             2747..3283
                     /locus_tag="VVL53_001537"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU4183692.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            3298..4803
                     /locus_tag="VVL53_001538"
     CDS             3298..4803
                     /locus_tag="VVL53_001538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU4183693.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            4819..5697
                     /locus_tag="VVL53_001539"
     CDS             4819..5697
                     /locus_tag="VVL53_001539"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU4183694.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            5783..7189
                     /gene="atpD"
                     /locus_tag="VVL53_001540"
     CDS             5783..7189
                     /gene="atpD"
                     /locus_tag="VVL53_001540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU4183695.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            7200..7619
                     /locus_tag="VVL53_001541"
     CDS             7200..7619
                     /locus_tag="VVL53_001541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU4183696.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(7664..8677)
                     /locus_tag="VVL53_001542"
     CDS             complement(7664..8677)
                     /locus_tag="VVL53_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU4183697.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            8821..9987
                     /locus_tag="VVL53_001543"
     CDS             8821..9987
                     /locus_tag="VVL53_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4183698.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9977..11206
                     /locus_tag="VVL53_001544"
     CDS             9977..11206
                     /locus_tag="VVL53_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4183699.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
BASE COUNT         3322 a         2063 c         2469 g         3357 t
ORIGIN      
        1 gaaatagatt ttactgtcct gatcgatttg ttctgttctt gtttcatttt actatatgtt
       61 cgtaaatgaa agttctataa tgaagttagc cacccagccc tcctaaaaat tagatggttc
      121 aactagctga tagctcttgt aggcataaga aaacacctct gtgttatact tgttgttcaa
      181 ccacaaacac aagaaaggca cagagatgca agacaattat actacaaaag ccaaacattt
      241 gacaatcgat agccgtcgct taatcgaaag atggaaaaaa gaagggaaat caaatagaga
      301 aattgcctct ctgcttggaa aagttcctca aactatccac actgaaatca agcgtgggac
      361 agtccgacaa tgtcttggaa aagggcgctt caaagaggtt tattctgccg actatgctca
      421 acagtcttat gaaaataatc gcaagcgctc ggtcaagaaa tcaagcttga ccaaggaact
      481 aaaggaaaag attctccact atcataacca aaaattttcg cctgaaatga tggtgatggc
      541 taaaggggtt aacgtgggaa tttcaaccat ttactattgg attcatcatg gaaaattggg
      601 gttaagcaaa caggatctgc tttaccctag aaaaggaaaa gcgcttaaga aacaggctag
      661 caccaacttt aaacctgctg gtcaatccat cgaacagcgg tctgaagcta tcaatcttcg
      721 cttggagaat gggcattatg agattgatac ggttctactt acgagagcga aaaactactg
      781 cttgcttgtc ttgacggatc gaaagagtag acatcagatc atccgattga ttccaaataa
      841 aagtgctgag gtggtcaatc aggctctaaa actcatctta aaacaacaca agattctttc
      901 catcacggca gataatggaa cggaattcaa tcgcttgttt gatgtatttt ctgaggagca
      961 catctattat gcgcacccct atgcctcttg ggaaagggga actaatgaga atcacaacag
     1021 gctcattcgt agatggttac ctaagggaac caagaaaatg actcccaaag aagtcgcatt
     1081 catcgaaaag tggattaaca actatcctaa aaaatgcttg gactacaagt cacccagaga
     1141 agacttctgg atggctaact tgaacttgaa atttagcaaa atggaaataa tttttattaa
     1201 acgcttccaa tagcggttaa aagttgacaa atttaaattt taggactaca ctaagtaagt
     1261 aaattttatt ttaaaggaga atttgttatg aatttaacat ttttaggctt atgtattgcc
     1321 tgtatgggcg tatctgtcgg tgaaggttta ttgatgaatg gactgtttaa atcagtagca
     1381 cgccaaccag atatgctttc tgagtttcgt agtttgatgt ttttaggtgt tgcctttatt
     1441 gaaggaactt tctttgtaac tcttgtcttc tcatttatta tcaaataaat acatggaacg
     1501 agaagaaaag ggaggatttt agatggaaga aagtattaat ccaatcatct ctattggtcc
     1561 tgttatcttc aatctgacta tgttagccat gactttgttg attgtgggag ttatttttgt
     1621 ctttatttat tgggcaagcc gcaatatgac cttgaaaccc aaaggaaagc aaaatgtact
     1681 tgagtatgtc tatgactttg ttattggatt tacagaacct aacattggtt cgcgctacat
     1741 gaaagattac tcactctttt tcctttgttt attccttttc atggtgattg ccaataacct
     1801 tggcttaatg acaaagcttc aaacgatcga tgggactaac tggtggagtt cgccaaccgc
     1861 taatttacag tatgacttaa ccttatcttt tcttgtcatt ttgttgacac atatagaaag
     1921 cgttcgtcgt cgtggattta aaaaaagtat aaaatctttt atgagtcctg tttttgtcat
     1981 accgatgaat atcttggaag aatttacaaa cttcttatct ttggctttgc ggatttttgg
     2041 gaatatcttt gcaggagagg tcatgacgag tttgttactt cttctttccc accaagctat
     2101 ttattggtat ccagtagcct ttggagctaa tttggcttgg actgcatttt ctgtctttat
     2161 ttcctgcatc caagcttatg tttttactct tttgacatct gtgtatttag ggaataagat
     2221 taatattgaa gaggaataga aaggagtaac tgatgcacgt aacagtaggt gaattaattg
     2281 gtaattttat tttaatcact ggctctttta ttcttttgct agtcttgatt aaaaaatttg
     2341 catggtctaa tattacaggc attttcgaag aaagagctga aaaaattgct tcagatattg
     2401 acagagctga agaagcccgt caaaaagcag aagtattggc tcaaaaacgc gaagatgaat
     2461 tggctggtag ccgtaaagaa gctaagacaa tcattgaaaa tgcaaaggaa acagctgagc
     2521 aaagtaaggc taatatctta gcagatgcta aactagaagc aggacactta aaagaaaaag
     2581 ccaatcaaga aattgctcaa aataaagtag aagctttaca gagtgttaag ggtgaggtcg
     2641 cagatttgac catcagctta gctggtaaaa tcatctcaca aaaccttgac agtcatgccc
     2701 ataaagcact cattgatcag tatatcgatc agctaggaga agcttaatgg acaagaaaac
     2761 agtaaaggta attgaaaaat acagcatgcc ttttgtccaa ttggtacttg aaaaaggaga
     2821 agaagaccgt atcttttcag acttgactca aatcaagcaa gttgttgaaa aaacaggtct
     2881 gccttctttt ttaaaacaag tggcagtaga cgagtcggat aaggaaaaaa caattgcttt
     2941 tttccaagat tctgtgtcgc ctttattaca aaactttatc caggttctgg cctacaatca
     3001 cagagcaaat cttttttatg atgtgcttgt agattgcttg aaccgacttg aaaaagaaac
     3061 aaatcgattt gaagtgacga ttacgtctgc tcatcctcta actgatgaac agaagactcg
     3121 tttgctccct ttgattgaga aaaaaatgtc tctgaaagta aggagtgtaa aagaacaaat
     3181 cgatgaaagt ctcattggtg gttttgtcat ttttgccaat cacaagacaa ttgatgtgag
     3241 tattaaacaa caacttaaag ttgttaaaga aaatttgaaa tagaaagtgg tgttcttttg
     3301 gcaattaacg cacaagaaat cagcgcttta attaagcaac aaattgaaaa tttcaaaccc
     3361 aattttgatg tgactgaaac aggtgttgta acctatatcg gggacggtat cgcgcgtgct
     3421 catggccttg aaaatgtcat gagtggagag ttgttgaatt ttgaaaacgg ctcttatggt
     3481 atggctcaaa acttggagtc aacagacgtt ggtattatca tcctaggtga ctttacagat
     3541 atccgtgaag gcgatacaat ccgccgtaca gggaaaatca tggaagtccc tgtaggtgaa
     3601 agtctgattg gtcgtgttgt ggatccgctt ggtcgtccag ttgacggtct tggagaaatc
     3661 cacactgata aaactcgtcc agtagaagca ccagctcctg gtgttatgca acgtaagtct
     3721 gtttcagaac cattgcaaac tggtttgaaa gctattgacg cccttgtacc gattggtcgt
     3781 ggtcaacgtg agttgattat cggtgaccgt cagacaggga aaacaaccat tgcgattgat
     3841 acaatcttga accaaaaaga tcaagatatg atctgtatct acgtcgcgat tggacaaaaa
     3901 gaatcaacag ttcgtacgca agtagaaaca cttcgtcagt acggtgcctt ggactacaca
     3961 atcgttgtga cagcctctgc ttcacaacca tctccattgc tcttcctagc tccttatact
     4021 ggggttgcta tggcggaaga atttatgtat caaggtaagc atgttttgat tgtatacgat
     4081 gatctatcaa aacaagcggt agcttatcgt gaactatcgc tcttgcttcg tcgtcctcca
     4141 ggtcgtgaag ccttcccagg ggatgttttc tatctccaca gccgtttgct tgagcgctca
     4201 gctaaagttt ctgatgaact tggtggtgga tcaattacag ccctaccatt tatcgagaca
     4261 caagcaggag atatctcagc ctatatcgca accaacgtga tttctatcac tgatggacaa
     4321 atcttccttg gcgatggcct cttcaatgca ggtattcgtc cagccatcga tgcgggttca
     4381 tctgtatctc gtgtaggtgg ttctgcacaa atcaaagcca tgaagaaggt tgctggtaca
     4441 cttcgtatcg accttgcttc ataccgtgag ttggaagcct ttactaagtt tggttctgac
     4501 ttggacgcag caacacaggc taagttgaac cgtggacgtc gtaccgttga ggtcttgaaa
     4561 caacctgttc acaaaccatt acctgttgag aaacaagtaa ccattcttta tgctttgaca
     4621 catggtttct tggatactgt tccagtagat gatattgttc gtttcgagga agagttccat
     4681 gccttctttg atgctcaaca tccagagatt ttggaaacca ttcgtgatac aaaagacttg
     4741 ccagaagaag cagtcttgga tgctgcgatt acagagtttc tcaatcaatc tagcttccaa
     4801 taagaataga ggtgtcagat ggcagtgtct ctaaatgata ttaaaacaaa aatcgcctca
     4861 acaaaaaata cgagtcaaat cactaatgcc atgcaaatgg tatcggctgc taagttaggt
     4921 cgctctgaag aagctgctcg caacttccaa gtttacgctc agaaagtgcg taaacttttg
     4981 acagatatcc ttcatggtaa tggagctggt gcttcaacta atccgatgtt gattagccgt
     5041 tctgtgaaga agacaggcta tatcgttatc acttcagacc gcggtttggt tggaggttat
     5101 aattcctcta ttttgaaagc tgttatggag ttgaaagaag aataccaccc agacggtaaa
     5161 ggttttgaaa tgatctgtat cggtgggatg ggagctgatt tctttaaggc tcgcggtatt
     5221 caaccacttt atgaattacg tggcttggca gaccaaccta gctttgatca agttcgtaag
     5281 attatttcaa aaactgttga aatgtaccaa aatgaactct ttgatgagct ttatgtttgc
     5341 tacaaccacc atgtcaatac gctaaccagt caaatgcgtg tggaacaaat gcttccgatt
     5401 gttgacttgg atccaaatga agcggatgaa gagtacagct tgacttttga attggaaacc
     5461 agccgagaag aaattctgga gcagttgttg cctcagtttg cagaaagtat gatttacggt
     5521 gccattatcg atgccaagac agctgagaat gctgcgggta tgacagccat gcaaacagcg
     5581 acagataatg ctaagaaagt catcaatgat ttgacaattc agtataaccg tgccagacag
     5641 gcggcgatta cacaagaaat tacagaaatc gtagcaggtg ctagtgcctt agaataggct
     5701 ctagtccagc tcgtatgaaa atgaacttag gacctagttg agctaggaac cgacagtatc
     5761 ttatatagaa taggagaagg agatgagttc aggtaaaatt gctcaggtta tcggtcccgt
     5821 tgtagacgtt ttgtttgcag caggggaaaa acttcctgag attaacaatg cacttgtcgt
     5881 ctacaaaaat gacgaaagaa aaacaaaaat cgtccttgaa gtagccttgg agttaggaga
     5941 tggtatggtt cgtactatcg ccatggaatc aacagatggg ttgactcgtg gaatggaagt
     6001 attggacaca ggtcgtccaa tctctgtacc agtaggtaaa gaaactttgg gacgtgtctt
     6061 caacgttttg ggagatacca ttgacttgga agctcctttt acagaagacg cagagcgtca
     6121 gccaattcat aaaaaagctc caacttttga tgagttgtct acctcttctg aaatccttga
     6181 aacagggatc aaggttattg accttcttgc cccttacctt aaaggtggta aagttggact
     6241 tttcggtggt gccggagttg gtaaaactgt cttaatccaa gaattgattc acaacattgc
     6301 ccaagagcac ggtggtattt cagtatttgc tggtgttggg gaacgtactc gtgaggggaa
     6361 tgacctttac tgggaaatga aagaatcagg cgttatcgag aaaacagcca tggtctttgg
     6421 tcagatgaat gagccaccag gagcacgtat gcgtgttgcc cttactggtt tgacaatcgc
     6481 tgaatacttc cgtgatgtgg aaggccaaga cgtgcttctc tttatcgata atatcttccg
     6541 tttcactcag gctggttcag aagtatctgc ccttttgggt cgtatgccat cagccgttgg
     6601 ttaccaacca acacttgcta cggaaatggg tcaattgcaa gaacgtatca catcaaccaa
     6661 gaagggttct gtaacctcta tccaggctat ctatgtgcca gcggatgact atactgaccc
     6721 agcgccagca acagccttcg ctcacttgga ttcaacaaca aacttggaac gtaagttggt
     6781 acaattgggt atctacccag ccgttgaccc acttgcttca agctcacgtg ccttggcacc
     6841 tgaaatcgtt ggagaagagc actatgcagt tgctgctgaa gtaaaacgtg tccttcaacg
     6901 ttaccatgaa ttgcaagata tcattgctat ccttggtatg gatgagcttt ctgatgaaga
     6961 aaagaccttg gttgctcgcg cccgtcgtat ccagttcttc ttgtcacaaa acttcaacgt
     7021 tgcggaacaa tttactggtc agccaggttc ttatgttcca gttgctgaaa ctgtacgtgg
     7081 ctttaaggaa atccttgatg gtaaatacga ccacttgcca gaagatgcct tccgtggtgt
     7141 aggttctatc gaagatgtga ttgcaaaagc tgaaaaaatg ggattttaag aggtgatcta
     7201 tggctcagtt aactgtccag atcgtgacac cagatggtct cgtctatgat caccatgcca
     7261 gctatgtatc ggttcgaact ctggatggtg agatggggat cttgccacga catgaaaata
     7321 tgattgcggt tttagcagtt gatgaagtaa aggtaaaacg tatcgatgat aaagatcacg
     7381 tgaactggat tgcagtaaac ggtggcgtta ttgaaattgc caatgatatg atcacaatcg
     7441 tcgctgactc tgcagaacgt gctcgtgata tcgatatcag tcgtgcagaa cgtgccaaac
     7501 ttcgtgcaga acgtgcaatt gaagaagcac aagacaaaca tttgattgac caagaacgtc
     7561 gtgctaagat tgctttgcaa cgtgctatta accgtattaa tgtcggaaat agactataag
     7621 aaaaaatgaa cttgaaaata ccaagttcat tttttatggt gttttaagga gcaaaacgga
     7681 tgcagactgc ttcgggaaca tggaagtcgt tggagagttc tgctagacga ccattgtcac
     7741 aattacgttt aaagacagtt gcattgtcag agtcttgatg gacaacaatg agaaattttt
     7801 ggtcgggtgt caaatcaaaa tcacgtggag tctgaccatg cgttggaacg atttctaata
     7861 actctaagct accgtccgca aggatggtat atactgcgat agaatcatgg ccacggttag
     7921 aagcgtagag gtatttaccg tctttagaga gatgaatagc agcggttcca ttaaagcctt
     7981 cgtaagcttc cggtaaagtt gaaatgacct gcatacgttc aaattcgcca acgccatcgt
     8041 agattaaaac ttcgatagta ctattgagtt cacaaatgag ataagcgatt ttatagtggt
     8101 tatggaaaat gatatggcgt gagcctgctc ctggcttgct gtgataggta tagagcttag
     8161 ataattttcc ttcttgatcg aggtcatagg tgatgacttg gtcagttccc aagtcgcagg
     8221 tcactagata gtggtcaggt gttaaatctg tatagtgaac atggggggaa gcttgatttt
     8281 catgtggacc ttggccactg tgttgatcca tatcactaag tagaagacta ccatcttcct
     8341 ggcgtttata aacaaggact tgtcccttgt gatagttagc tgcgtaaacc aaatcacgct
     8401 tttcatcgac agcaacataa cagtggggag ctccttcttc aacaacatga tttaacacag
     8461 tcccgtcagt ttgataggct gcaattcccc ccttatcgtc ttggctacca acagtgtata
     8521 aatgttggtg ctggtcaaag gcaaggtagg ttggacttgg ctcagctgca aaaagttcta
     8581 gatttgaaag ctgaccagtt tctgtatcaa agtctgcctt gtaaatccct tgagaagtac
     8641 gacgtgtata agttccaaaa taaacagttt ctttcattac tatacctctg tgtaaagata
     8701 agactattat atcacaaaaa caagtaaatt aaagatatcc aattagatgt aagcacttta
     8761 aaaaagagtt attttgtttc aaaaatggta taatgagaga acaatagaaa ggaagtattt
     8821 atggagcaaa aagagaaaca ttttagccta tcttggtttt tcaagtggtt tttagataac
     8881 aaggcaatta cggtattttt agtaacctta ttattgggac tgaatctttt tattttaagt
     8941 aagattagtt ttctattttc acctgtttta gactttttag cagttgtgat gttgccagtc
     9001 attttgtctg gtttgttata ttatttgttg aatcctattg ttgattggat ggagaagcat
     9061 aaggttaatc gtgttatagc tatcactatt gtctttgtta tcatcgctct ctttatcatt
     9121 tggggcttgg cagtcgccat tccaaatctg caacgtcagg ttttgacctt tgcaagaaac
     9181 gttcctgttt acttagaaga tatagatagg attgttaatg gattggtagc ccagcacctg
     9241 ccagatgatt tcagacctca attagagcaa gttttgacca atttttctag ccaggctaca
     9301 gttttggcaa gtaaggtttc atctcaggca gtcaactggg tgagtgcctt tattagcggg
     9361 gcttctcaag tgattgttgc cttgattatc gttcctttca tgctctttta tctcttgcgt
     9421 gatgggaaag gcttgcgtaa ctatttgacc caattcattc caagaaaatt gaaggaacct
     9481 gttggacaag ttttatcaga tgtgaatcaa cagttgtcca actatgttcg agggcaagtg
     9541 acagtggcta ttattgtagc agtaatgttt atcatcttct tcaagattat tggtctacgc
     9601 tatgcggtta cgctgggggt tactgctggt attttaaatc tggtccctta tcttggtagc
     9661 tttctagcca tgcttcctgc tctagtattg ggtttgattg ctggtccagt catgcttttg
     9721 aaagtagtga ttgtctttat cgtagaacaa actattgaag gccgttttgt ctctccattg
     9781 attttgggaa gtcaattaaa catccaccct attaatgttc tctttgtttt gttaacttca
     9841 ggatctatgt ttggtatctg gggagtttta cttggtattc cggtttatgc ctctgctaag
     9901 gttgtcattt cagccatttt cgaatggtat aaggtagtca gtggtctata tgaattagag
     9961 ggtgaggaag tcaagagtga acaatagtca acagatgtta caggctttgg aggagcaaga
    10021 tttaactaag gctgagcatt atttcgccaa agctttagaa aatgattcaa gtgatcttct
    10081 gtatgaattg gcaacttatc ttgaagggat tggtttctat cctcaggcca aggaaattta
    10141 cctgaaaatt gtagaggatt ttccagaggt tcatcttaat ctagctgcaa ttgctagcga
    10201 ggatggtcaa atagaagaag cctttaccta tcttgaggaa atccaagctg acagtgactg
    10261 gtatgtctcg tctttggctc tgaaggcaga cctttaccag ctggaaggtt tgacagatgt
    10321 ggcacgtgag aaattattgg aggccttgac ctactcagag gattctctct tgatattggg
    10381 tttggcagag ttggatagtg agttggaaaa ttaccaagcg gctattcaag cctatgccca
    10441 gttagataat cgctcgattt atgagcaaac gggcatttcc acctatcaac gaattggctt
    10501 tgcctatgct cagttaggga aatttgaaac ggctactgag tttttagaaa aagccctgga
    10561 gttagaatac gatgacttaa cagcttttga gttggccagt ctttattttg atcaagaaga
    10621 atatcaaaaa gccaccctct actttaagca gcttgatacc atttctcctg actttgaagg
    10681 ctatgagtat gggtacagtc aggctttaca taaggaacat caagttcaag aagccctgcg
    10741 tatcgctaag caaggattag agaaaaatcc ctttgaaact cgcctcttgc tagctgcttc
    10801 acaattttct tatgaattgc atgatgctag tggtgcagaa aattatctcc ttactgcaaa
    10861 agaagacgct gaggatacag aagaaatctt gcttcgttta gccactattt atctggagca
    10921 ggagcgttat gaggatattc tagaattgca gagtgaggag ccagaaaatc ttttgaccaa
    10981 gtggatgatt gctcgttctt atcaagaaat ggacgatttg gatactgctt atgagtatta
    11041 tcaagagttg acaggagatt tgaaggacaa tccagaattt ctggaacact atatctatct
    11101 cttgcgtgaa ttgggacatt ttgaagaagc aaaagtccat gctcacactt acttaaaact
    11161 ggttccagat gatgtgcaaa tgcaagaact gtttgagaga ttgtaagaat g
//
LOCUS       DAVHEW010000067        11129 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVHEW010000067 DAVHEW010000000
VERSION     DAVHEW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.067"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..1096
                     /locus_tag="VVL53_001545"
                     /pseudo
     CDS             1..1096
                     /locus_tag="VVL53_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1224..2246)
                     /gene="cbpF"
                     /locus_tag="VVL53_001546"
     CDS             complement(1224..2246)
                     /gene="cbpF"
                     /locus_tag="VVL53_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEU4183700.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLNPATGI
                     MQTGWQYLGNRWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQESSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(2265..3086)
                     /gene="cbpG"
                     /locus_tag="VVL53_001547"
     CDS             complement(2265..3086)
                     /gene="cbpG"
                     /locus_tag="VVL53_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU4183701.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(3167..3406)
                     /locus_tag="VVL53_001548"
     CDS             complement(3167..3406)
                     /locus_tag="VVL53_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183702.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3556..3822)
                     /locus_tag="VVL53_001549"
                     /pseudo
     CDS             complement(3556..3822)
                     /locus_tag="VVL53_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3847..4101)
                     /locus_tag="VVL53_001550"
     CDS             complement(3847..4101)
                     /locus_tag="VVL53_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU4183703.1"
                     /translation="MAANYLKAMQSYLKETDLPKLEQLVVTKSLWDTVDILDRVVGSL
                     VYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLAS"
     gene            complement(4083..4324)
                     /locus_tag="VVL53_001551"
                     /pseudo
     CDS             complement(4083..4324)
                     /locus_tag="VVL53_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(4325..4957)
                     /locus_tag="VVL53_001552"
     CDS             complement(4325..4957)
                     /locus_tag="VVL53_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183704.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4971..5966)
                     /locus_tag="VVL53_001553"
     CDS             complement(4971..5966)
                     /locus_tag="VVL53_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4183705.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5963..6661)
                     /locus_tag="VVL53_001554"
     CDS             complement(5963..6661)
                     /locus_tag="VVL53_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU4183706.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLIFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6738..7748)
                     /locus_tag="VVL53_001555"
     CDS             complement(6738..7748)
                     /locus_tag="VVL53_001555"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU4183707.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7732..8739)
                     /locus_tag="VVL53_001556"
     CDS             complement(7732..8739)
                     /locus_tag="VVL53_001556"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU4183708.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVTSLVEALEQGKSEKIIDQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQTVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8726..9679)
                     /gene="mvaD"
                     /locus_tag="VVL53_001557"
     CDS             complement(8726..9679)
                     /gene="mvaD"
                     /locus_tag="VVL53_001557"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU4183709.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9661..10539)
                     /gene="mvk"
                     /locus_tag="VVL53_001558"
     CDS             complement(9661..10539)
                     /gene="mvk"
                     /locus_tag="VVL53_001558"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU4183710.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10714..10992)
                     /locus_tag="VVL53_001559"
     CDS             complement(10714..10992)
                     /locus_tag="VVL53_001559"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4183711.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
BASE COUNT         3314 a         2557 c         1989 g         3269 t
ORIGIN      
        1 tgaatacggt ctcccaagtc gtacgatact tcttaactgg ctagcacaat acaggaaaaa
       61 cgggtatact attgttgaga aaacaagagg gagagtacct aaaatgggac gtaagccaaa
      121 aacgagacct gaagagagga cagaattaga acgtcttcaa gcagaaaatg agtacctgag
      181 agcggagaat gccatcctaa aaaagttaag agaactccga ttgaaggagg agaaagaaga
      241 aagacagaaa ttgttcaaga attaatgact gaattttcgt tagctcttct tctaaaagtc
      301 attaaactag ctcgttcgac ctactactat cacttgaaac agctagataa accagataag
      361 gactaagagc ttaaagctga aattcaatcc atttttatcg aacacaaggg aaattatact
      421 tatcgccggg ttcatttaga actaagaaat cgtggttatc tggtaaatca taaaagaatt
      481 caaggcttga tgaaagtact caatttacaa gctaaaacgc gacagaaacg aaaatattct
      541 tctcataaaa gacgttggca agaaggcaga gaatctcatt caaggccaat ttgaaggctc
      601 taaaacaatg gaaaagtgct acacagatgt gacagaattt gccattccag caagtactca
      661 aaagctttac ttatcaccag ttttagatgg ctttaatagc gaaattatcg cctataatct
      721 ttcaacttca cccaacttag aacaagtaca aacaatgttg gaacaggcat tcacagagaa
      781 gcactacgag aatacgattc tccatagtga ccaaggctgg caataccaac acgattctta
      841 tcatcggttc ctagagagta agggaattca agcatccatg tcacgtaagg gaaacagccc
      901 agacaatagt atgatggagt ccttctttgg gattctgaaa tcggaaatgt tttacggtta
      961 tgagaagtcg tttcagtcgc ttaagcaatt ggaacaagcc attatagact atattgatta
     1021 ctacaacaat aaacgaatta aggtaaaact aaaaggactt agccctgtgc aatacagaac
     1081 taaatccttc ggataaatta attgtctaac tgtttgaggg cagtacagaa aaaccttgat
     1141 actatgcgtt ttttgattgt gaagactttt atccacctat tatacaaagt taagtatcca
     1201 tcacagttta caattagcct tcattactta acccattcac cattatagtt taagtagtaa
     1261 ccacctactg tggtattaac agctaaagca cctgaatcat aggcatagta ccagttacca
     1321 ttgacttgga accagcctgt tttcatatct ccattacttg catttagata gtaccaagtc
     1381 gaactttcct gataccaacc agttgccata gctcctgatg aacggagata gtaccatttg
     1441 ttcccaaggt tttgccagcc agttctcata tcaccatttt cagcatctag atagtaccaa
     1501 gttgagcctt ccttatacca gccagttgcc atagctcctg acgaatggag gtagtaccat
     1561 ctattaccta gatattgcca acctgtttgc ataatgccag ttgctggatt tagatagtac
     1621 catggagctg cttttagctt ctcttcatca tacgtaagag ggtaatcctt aacccaacca
     1681 cgtgctagct ctccaaccgt caatctgttg atgcgcgaat caaagccacc atccttctgt
     1741 aaataatacc aatgaccctc ttcataaatc caaccagttt ttaaagtatg ataactacgc
     1801 tgatcttcaa aataatagac tcgtttctct gcttggctat catattcttc cccatgatgt
     1861 ttgttggtat tcgtagcagt ttttgcttct aaaacttgct tgccaacaaa ttcttgtaat
     1921 acaccatctt gaccaaaata aaaccaatct ggtctaagag caatctctat tcttggagaa
     1981 ggaccaatcg taaccccctt gtgtggagca ggtatatatt gccagccgac aaccatctct
     2041 ccggataaag gatcaaaata atagtacttc ccatctatca ctctccagta ggtttctttt
     2101 agatccccct ttttgtagta ggttctacca ttttcttgga caaactgcca tccttcagaa
     2161 tcatctgcaa atactgtatt tgtcgctaac aaaccgaaga aaaatgtggc tagtgcgatt
     2221 tgcatcattt ttttcaaaag cttcatttta tatatcctcc aacattaaat ccactcacca
     2281 gatgaagcga agttataaac tttaccatcg atagtttggc tacctgtaac cattgctcca
     2341 tttgaattga gataatacca ttttccattt actttttgcc aatctgtaag catcttaccg
     2401 gaactgtcta aataatacca agtatcattt atctcctgcc aacccgtttg tttatcatgt
     2461 tgtctataat ggtaccaact accatttatt ttcttccatc cttcaagaga gtaaccttta
     2521 agaaccgaat aaataaatgg caaatttcgt tcatttaatt taactgcact gttaatttga
     2581 ttagctccat ctcctaaagt atgcactcct actacacggt gactagcatc ataaactgta
     2641 gatccactag acccctctaa agtatcaact tggtaatcca agaacatgcc atcatcactt
     2701 aaaacttgtt ttttatctgt atacatttga tgaattttaa aattatatga tggatagcct
     2761 gtgatagtca cagttattcc tgtcaaattt ttttgactag taggaagacc caaagtccct
     2821 aattttgcac caatgggctc ttctagaatt aataaagcca agtcatattc ccttgcatcc
     2881 ttagaattta aatttctaaa ttccttcaaa taacgaactt cctttacttt gatctttcca
     2941 aatggttctt gacttggact aacagccgga agaacataaa tatcatccgc ttctttccca
     3001 tagtcatgtc tgtagtagtt gtgagccgct gtaattaaca cattatcttt aataaaattc
     3061 gcacttcccc tcccttctac attcattcca tcggctactc cataatactt agataaaacc
     3121 atagctgaag tcgaatacgg atactgtaaa gtattatcaa ttttaatcaa atcatcatta
     3181 ccgataatac ttctgattgc ttttggtagt atgaaccata cgttggtgaa atctcagata
     3241 atgaagaatc attagactct ggaccttttt ctagtgtctc acttacctca tattcttcac
     3301 ctgtactaga aataacactc aaagcagata ctgtcgataa ctggctagcc aataaagtac
     3361 tcgcaataat tgaaataccc aattttttat aaacagtttt cttcattatt gtatcctcct
     3421 aatgtaatta tagcgtacta ttctaaattt cttaatctac tatagaatca agaaatctac
     3481 caccttcttt aaataccctc cattatcaca taaacaggta aacttttcaa ttaatgactg
     3541 cgcttttcaa tcacgctaga ggtacttgct tgcttctttg atactaagtt cagccattct
     3601 ttccttgttt ttctcaataa agcatgttac ccaagtggga ttcgttttgg agtagtctcg
     3661 cagagtccag ccaatggctt tattgataaa aaattctgtt tggttcaagt tatgaaggag
     3721 aatcttttcc attaattgag tattggtctt ctcttttctt aacaactggt ggtcaatagc
     3781 gacacgtctc agccagatat tatctgatag gctccatttt atactcaatg aaaatcaaag
     3841 agcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt gcagatagag
     3901 ctgacgtggt ttgaagagat tttcgaagag tattaagatt atttcttcta gttcagggtg
     3961 ttcatacacc aaactcccta ctactcgatc taggatatct accgtgtccc acaaggattt
     4021 tgtcacgact aactgctcta gcttaggcaa atcggtttcc tttagataag actgcattgc
     4081 tttcaaatag ttagcagcca catattggta ttttctagga tccttttccc agcaagtgtc
     4141 tgcaaaatcc caatcgataa tctttgtttt tttcgcttct ggaaaatatt ttatagagtt
     4201 tatttctttc aggcaccgca atacctagaa aagaaaattg atggcgcata taggcttcca
     4261 tggaccttgc ttttttagag tcttttgctg cttctagctc ctcaagtaaa tctgctaaac
     4321 tcatctaaaa ctcctcttgc cccaccaaat ggtgctgaaa ggcatagaca gccgcctggg
     4381 tacgatcgct gacttcaagt ttggcaagaa tattggacac gtgggtcttg accgtcttga
     4441 gagagataaa gaggtcatct gcgatgcgct gattttcgta gcccttggcg atgagttgga
     4501 gaacatctcg ctcacgcgca gtcaattctt catgaagttc catatgattg cggtggtatt
     4561 caaccttctt gctaacctct tgctcaatgg ccagctcgcc agcagctacc ttactgacgg
     4621 catgaagcaa ttcatctgca ctagaagtct tgagcatata gcctttggca ccagcatcta
     4681 agactggcat gattttttca ttgtccaaat aagaggtcac aatcaaaatc ttggcttcag
     4741 gccattcttt aaggattgct aaggtcgcgt caatcccatt catctcaggc atgacaatat
     4801 ccatgacaat gacatctgga cgcagttcca aggccaagtc aatcccttga gacccgttgg
     4861 acgcctcacc cacaacttct acatcgtctt ggaggtcaaa gtagcttttc aagcccaatc
     4921 ggaccatttc atggtcatct actagtaaaa ttttcatctt tactccttta tcattcctta
     4981 tctaacaggg gaatacggat atcaaccgcc agcccttgct tgggagctgt caagagttga
     5041 actgttccag ccatatcttc aacccgctcc ttgatatttc gcagtccata actcaagtcg
     5101 tctaagctcc ctaactggaa accaatccca ttgtccacca ccttcagttg caattcaaca
     5161 tctgtctgat agaggtagac atctaggcaa gatgcctggg catggcggag ggtattgcta
     5221 atcaactctt gcaggatacg gaagatatgc tcctcgattt tcttaggcaa tttcgtcata
     5281 ttctgcttga gactaaccct aagatcactc ttgtcctcaa gctcttttaa aagaatttga
     5341 atcccttcta tcaagctctt ctgctccagt tcaactggtc gcaaatgcaa gagcaaaacc
     5401 cgcaaatcct tctgggctgt ttctaaaata gctgtgacac tctgcaactg ggtctgcatc
     5461 ttttctctat ccaatttcaa agcctgctga ctgatacccg ataaaatcat gtgggccgca
     5521 aacaactcct gactgactgt atcgtgcaaa tcccgagcaa ttcgcttccg ttccttctcg
     5581 atgatttcct cttcctgagc aaggctctga ttttcagctt tttgaagagc ctctgtcaaa
     5641 aggttaagtt tacctgataa ggacttgaaa ctggcatcca aatctggatc tgcaacctga
     5701 accacttctt gccctgctaa taaacgcttg agattagcct gcatttttct tagagaaagc
     5761 tcttcgatcc ctcgccaaaa cagggctaag agacaggtca tggacatgct gaaaaccaac
     5821 aataaaaaga caaatttttc tgttttttcg acatcgtgca aaaagataga ccagtcaaaa
     5881 tcaagtattt ccagcaagct gtgggagaaa aaaaagacaa ataggaagga ggtgagagca
     5941 ataatgacat aggcttgttt tttcatcctc taaccacctc cacatcacca atcatagtgg
     6001 tcaagaaaat cttgacactc ttgttactct tgagatagtc ttttgtttct tgatgatagt
     6061 gttcattgcg gagggctcgc ttgggctggt tgaaaaaaat caaatcccca tagagacagt
     6121 taacgctgag actgacttcc acatctacag gtacgatgat tttggtcgtt cctaccatct
     6181 ttctgaggat aatgacattg tcatgattgg ttaagatgac cctctccaga tgaatagtgt
     6241 ccttgcccat gaagcgaaag agattgatat catcgaattg gcaagtctgg tagcttgaaa
     6301 aatgatgaag atttccaaac caacgatttt tctccttctt aaccgtcacg acctcttcaa
     6361 aaaccaaatt ggtctgctct ttttcctggt tcatcatcgg gtaaagaaga aagaggctat
     6421 agataaccgc aacaaaaata gctagaatca caaaaggatt gagcataacg atgaaaaaga
     6481 agagaatggt tgccgctact aaaagaagat tatttccctc tttaccagtg tagtagcgaa
     6541 tcaaaagcaa aaagaggaat agtatcagca gaaaacgcga aaaatgctct gataccatca
     6601 aaatcagagc tcctgtcaga agacaggctt cgataaataa aaagatttta aattttctca
     6661 taggttcatc ctctcccttc tattttatca caattcaaaa aagtcacctc agtctgagga
     6721 tggaaaaaag gcgctggtta cgcctttttc atctgatcct ttgcttcttt taattttcca
     6781 taaagaagat agtctacttt ttgtagatct gctatggtgg cacagttaag ggaacacata
     6841 atcaagcgta gatctgcttt ccagccttgg acaatgccaa tcacttcttc aactgtgtag
     6901 gtttcaacca attccagaac ggttcgtgac aatcccacag ccttagcacc aaaaaccaag
     6961 cacttaatca tatccagcgg attccgaacc cctccactaa ccaagagttc gaccttatct
     7021 ttccattctt gggcattgag aagggcctgc atggtagact gaccccattg attgaggtaa
     7081 tcacgctggc cactacgacg gttttcgata taggcaaagc tggtgccacc acgacccgat
     7141 aggtccactg tacgaacacc gaattcatag gctctttcga ttgtcttggc atccattcca
     7201 aagcccactt ccttgaggac aataggaacg ggaatttgct tgctataatc tgctagatgc
     7261 gattgccagc ttctaaactt cctttctccc tcgggcatga gtaattcctg catgacattg
     7321 acatgcactt gcaatagaac aggattcatc tcttctacag tctgaagtcc taactcgaca
     7381 ggcttgtcca atccaatatt ggttccaagg aggagattgg gatgactaga cttgacagaa
     7441 aaagaatcat ccgttggatt tttgagggct gcgctataag aacccgttac aaataaaata
     7501 ccacaggatt ccgccacctg agccagcttt tgattgattt ctcttccctt attacttcca
     7561 ccagtcatgg cattgatata aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa
     7621 agatcgattt catccagatt gtaaagaggc aaggaagaat gaatcagctc cacctcatca
     7681 aagctattat aggaactttt ctgctcaagg gcatagagga tatgctcgtc cttacgattt
     7741 gtcgtcatgt cctatccttt cttgatataa gagctcaatc cccagatcgg cccaacgatt
     7801 ttttaaggtt ttggttgatt gcgcatcaaa actcagggcg atgccacagt caccaccacc
     7861 agcaccacta ctcttggcaa cggtctgcaa atcttgactg gcttctttca actgtctaag
     7921 caaaggcgtg taaatatctg tactcaagcc ttctaaaagc ttgctggcta cttctacttg
     7981 atcgataatc ttttctgatt tcccctgttc caaggcttct accagagaag tcaccgtttc
     8041 ttttgaggaa gttaaaaaat tttgattgat attttgcttg atttgctgga ccatgtgact
     8101 cgatacagcc acttccttgg tccatcccac taagaaatca cattctaaag ttggtttcac
     8161 ttgtgaaatt gaaaagcccc aatcacgctc cagaactgtc gccaagtttt cttcttctaa
     8221 ccaagcagcc accttctggc gatcaaatga ctggtagaga accaaatcct ctgccacaat
     8281 acaggcaagg tcgcccatgg aaccattgtc tcctcgctta agcaagacag cgctagtcag
     8341 cttgaacaag agctcctgat caacagaaac atcatacaga gccagtaaag ccttgacaac
     8401 caagacaacg acgctgccac tagaacctag accaaacttt ttcccttctc gttccatttt
     8461 gccacagatt tctagagaaa aaggtcttaa attctgacca cgaacagcga ggaagtctcc
     8521 catcaaagca atcgtttctt gaatcaagct atagtcagga ttaggcctta agtccactgc
     8581 gaaatcaaac atatctgaat agatacggta gctgtcagaa aaagcaatct cagccctcat
     8641 atagatggga atatccttta tcaaagctaa ctgccctggc tctaaaatag catattcacc
     8701 tgcccaatag agttttccgc aagttttaac agcaatcatc ttgactcaaa tcctttgttt
     8761 ttgacacaat caagcgataa cgatgaccga aaatttctga taaatgctcc aagtctttct
     8821 cctgacagaa gaccttaaca ttgggaccag catccatggt aaagtagcag gcctctcctt
     8881 tctcacgaag ctggcgaaca aaggccatag cctcataaga ggcatccgtc agataagaaa
     8941 aggctggact agcagtcttt gtcgtagcat gcatagccag ggcatttttc tccgttaatt
     9001 ctccaatctt ggcaaaatca ttttccttga gataaatcag catatcctga tagtccttct
     9061 cagactgacg aacccagtcg tcgaaagtcg tcgaggtttc cacacaaagt ttcatcccgt
     9121 cacggctaga gattggtttt ttcttgtcct ctagcaccaa cataatcata gctagtttca
     9181 agtctgtctc tacagggtaa atttctccac tatccttatc ccaggctcct agtggtccat
     9241 aaaaactccg agaagaagaa cctgaggcaa atttggcttc ctgtgccaac tgacttctat
     9301 ccaatccaag cttgaaataa gcattacaag ccttgaccag ggcggacaaa ccactagaac
     9361 ttgaggacag acccgctgcc gtaggcatat tgttttgagt atcgatacgg acaaagccct
     9421 caccagctgg acgataacgg tcaataatct tactcatctt ggcatgctcg acctcatttt
     9481 gtagctgacc attgatgtaa aattcgtcag ctgttacatt ggctggtaaa ggcgacaagg
     9541 tcgtctctgt atacatattt tccaaagtta gagaaatact gctagtagca ggcaccatct
     9601 ctttttcttt tttctttccc caatatttga taatagcaat atttgcgtag gaacgtactg
     9661 ttacaggctc tctatccatg tctgaacagc tcctttctct tctaatcttt ctgctagttc
     9721 ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa cctcctagcc caccaccgct
     9781 catcttggca cccagagcac catggctaag agtcgtttca accaaaaagt ctgcctcagg
     9841 gctactgact ccaatttctt ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc
     9901 ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg
     9961 caaaaacggt agggcatcct tgcccttatt ttgaaccact tggatggctt cacgagtatg
    10021 accataaaca cccgtatcgg caatcaccaa ataggcggat aaatccatct caagttctgt
    10081 aaatcctacg ttcttgataa agcgaatagg ttggtcacta agacaggtct tagcatccaa
    10141 accactagga ttcatatggg caatcatttc agctcgattg accaagattt ctagtacatc
    10201 atgaggcaga tcagcctgat agtagtcaaa tactgcacga atggccgcta tgctgatagc
    10261 cgctgacgaa cccatccccc gtttctcagg gatagccgag tcaatctcac aacgaatgca
    10321 ggcttctgtg atattcaaat actccagtga ggcataaacc gccatggaca aggtatcctc
    10381 ctcataaagg cgccaaggac tctctgcagg aactacctta caggtcacct ccacctccaa
    10441 aagaggcagg gaaatggcag gataaccgta aacgaccgca tgttccccta ttaaaattat
    10501 cttactatgt gcctgaccga caccaacttt ttttgtcatt ttttcctttt actagacgaa
    10561 aaaacgtctt atttttcata caagtattaa ttctttccta tctattttat tatattttca
    10621 caaaaaaagc gattgtttcc attcacaatc gcttctttca ttattgaacc cattcgccat
    10681 tatagttgac agaatagcca tctacggtcg tattcactgc caaggcacct gagcgctata
    10741 agcgtagtac catctgccat tgacctggaa ccaacctgtc gtcatagaac gacgaaagaa
    10801 actccatacc attaagtaaa gaggaaagtc gtgagggagc atgcgccatt gacaacctgt
    10861 tttagtgacg tacaaagtct cattaacaag tactcgtttc ggccatttat aggtgcggtg
    10921 tttggagaaa tagggttcaa tcttcgccca ttcttgatcg tttaaatcag tatcatatgc
    10981 tttgcgtatc ataactctag cttaacattt ttttgtgaat acaggttcta aataatcgac
    11041 cacgaaaatt tcttaagtgg aaaacgcctt atgaagtatg ctacgggaaa gttatgcact
    11101 taatttgaca attcaagatg taaaaatat
//
LOCUS       DAVHEW010000068        11040 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVHEW010000068 DAVHEW010000000
VERSION     DAVHEW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.068"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            306..1469
                     /locus_tag="VVL53_001560"
     CDS             306..1469
                     /locus_tag="VVL53_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU4183712.1"
                     /translation="MNIKEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMEGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDALDQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQVSPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAINNL"
     gene            complement(1623..2927)
                     /gene="eno"
                     /locus_tag="VVL53_001561"
     CDS             complement(1623..2927)
                     /gene="eno"
                     /locus_tag="VVL53_001561"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU4183713.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            3091..3537
                     /locus_tag="VVL53_001562"
     CDS             3091..3537
                     /locus_tag="VVL53_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU4183714.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3534..4649)
                     /locus_tag="VVL53_001563"
     CDS             complement(3534..4649)
                     /locus_tag="VVL53_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU4183715.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASFYLERTAANIGHLLNMCKI"
     gene            complement(4658..4891)
                     /locus_tag="VVL53_001564"
     CDS             complement(4658..4891)
                     /locus_tag="VVL53_001564"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU4183716.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAKLG
                     IAFCAKEVLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            complement(4932..5303)
                     /locus_tag="VVL53_001565"
     CDS             complement(4932..5303)
                     /locus_tag="VVL53_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU4183717.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(5362..6795)
                     /gene="glgA"
                     /locus_tag="VVL53_001566"
     CDS             complement(5362..6795)
                     /gene="glgA"
                     /locus_tag="VVL53_001566"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU4183718.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6792..7931)
                     /gene="glgD"
                     /locus_tag="VVL53_001567"
     CDS             complement(6792..7931)
                     /gene="glgD"
                     /locus_tag="VVL53_001567"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU4183719.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7921..9063)
                     /locus_tag="VVL53_001568"
     CDS             complement(7921..9063)
                     /locus_tag="VVL53_001568"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU4183720.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIINTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(9053..10981)
                     /gene="glgB"
                     /locus_tag="VVL53_001569"
     CDS             complement(9053..10981)
                     /gene="glgB"
                     /locus_tag="VVL53_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU4183721.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSAIKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3412 a         2392 c         1968 g         3268 t
ORIGIN      
        1 ggcaatgata ttcctaattc gtctttaaaa aaaattgact atatcgcacc ttcagaaatt
       61 gtttcattta gtacgtacgt tcgacaacgt tctaaagtaa ttcctaaaat tttggaacat
      121 atattaaaat caagtttttt attagagaat atagatgttt ctggttacac tgtaaatatt
      181 ttagaagatc aattaacaaa acatagaaca atcaaaatta gtaaaaacta actggttgat
      241 ctcatgtata aatacctaac aaaaccacgc gccttgcctg ctgatggaaa gaaaggtaca
      301 aatacatgaa tatcaaagaa aaaatcaaaa agaatggcca aagagtttat tatgctagtg
      361 tttatctagg cgttgaccaa ctaacgggca aaaaagcccg tacaactgtt acagcaacca
      421 ctaaaaaggg cgttaaagta aaagcgcgtg atgcgatcaa tacttttgct gctaatggct
      481 atacagttaa agacaagccg acaattacaa catataatga gcttgtaaaa gtttggtggg
      541 atagttacaa gaatacagtt aagccaaata ctcgccaatc catggaggga ttggttagag
      601 tgcatttatt gcctgtattt ggcgattaca agctatctaa acttactacg cctattcttc
      661 aacagcaagt aaacaaatgg gctgacaagg caaataaagg cgaaaaaggg gcatttgcta
      721 actactcttt gctccataac atgaataagc gtattttgaa atatggcgta gctatccagg
      781 taatacaata caacccagct aatgatgtca tcgttccacg caaacagcaa aaagaaaagg
      841 ctgctgtcaa atacttagac aacaaagaat taaaacagtt tcttgattat ttagatgctc
      901 tggatcaatc aaattatgag aacttatttg atgttgttct gtataagact ttattggcca
      961 ctggttgccg tattagtgag gctctggctc ttgaatggtc tgatattgac ctagaaagcg
     1021 gtgttatcag catcaataag acactaaacc gctatcagga aataaactca cctaaatcaa
     1081 gcgctggtta tcgtgatata ccaatagaca aagccacatt acttttactg aaacaataca
     1141 aaaaccgtca acaaattcag tcttggaaat taggccgatc tgaaacagtt gtattctctg
     1201 tatttacgga gaaatatgct tatgcttgta acttacgcaa acgcctaaat aagcattttg
     1261 atgctgctgg agtaactaac gtatcatttc atggtttccg ccatacacat actactatga
     1321 tgctctatgc tcaggttagc ccgaaagatg ttcagtatag attaggccac tctaatttaa
     1381 tgatcactga aaatacttac tggcatacta accaagagaa tgcaaaaaaa gccgtctcaa
     1441 attatgaaac agctatcaac aatttataaa aaataagggt gacccatttc cgggctaccc
     1501 tcttactata ccaaaaatta gtaggggtag taaaaagggt attaaattat aaaaagcact
     1561 aagggaaagc gccccaaagt gcttatttca aaggctttat agcctataat cacataaaga
     1621 gattattttt taaggttgta gaatgatttc aatccacgat attcagctac ttcaccaagt
     1681 tggtcttcga tacgaagcaa ttggttgtat ttagcgatgc ggtctgtacg tgaaagtgaa
     1741 ccagtcttga tttgtcctgc gttagttgca actgcaatat cagcgattgt tgaatcttca
     1801 gtttcacctg aacggtgtga tacaacagca gtgtaaccag cttctttagc catttcgata
     1861 gcttcaaaag tttcagtaag agtaccgatt tggttaactt tgataaggat tgagttagca
     1921 gcaccttctt ggataccacg tgcaaggtag tcagtgtttg ttacgaagaa gtcgtcacca
     1981 acaagttgta ctttcttacc aagacgttca gtaagagctt tccaaccatc ccagtcgttt
     2041 tcatccatac catcttcaat agtgatgatt gggtatttgt taaccaattc ttcaaggtag
     2101 tcgatttgtt ctgcagatgt acgaacagca gcaccttcac cttcaaattt agtgtagtcg
     2161 taaactttac gttctttatc gtagaattct gatgaagcac agtcaaatcc gataaatacg
     2221 tctttacctg gtacatatcc agcagcttca atcgcagcaa ggatagtttc aacaccatct
     2281 tcagttcctt cgaaacgagg agcgaatcca ccttcgtcac ctacggcagt ttccaaacca
     2341 cgtgatttaa ggattttctt aagagcgtgg aagatttcag caccgtaacg aagggcttct
     2401 ttaaatgttg gcgcaccaac tggcaagatc atgaactctt ggaaagcgat tggagcgtca
     2461 gagtgagaac caccgttgat gatgttcatc attggagttg gaagaacttt agtgttgaat
     2521 ccaccaagat agctgtaaag tgggatttca aggtagtcag cagcagcacg agctacagcg
     2581 atagacacac cgaggattgc attcgcaccc aatttacctt tgttaggagt accgtcaagt
     2641 gcgatcatag cacggtcaat agcttgttga tcacgtacat cgtagccaat gatagcttca
     2701 gcaatgatgt tgtttacgtt gtcaacagct ttttgtgtac caagaccacc gtaacgagat
     2761 ttgtcaccgt cgcgaagttc aactgcttcg tgttcaccag tagaagctcc tgatggaacc
     2821 ataccacgtc cgaaagcacc tgattcagtg taaacttcta cttcaagtgt tgggttaccg
     2881 cgtgagtcta ggacttcgcg agcgtaaaca tcagtaataa ttgacatttt ttactctcct
     2941 tatgagttaa attttttaca cctctataat accttaaaac ccctcctttt tcaagaaaaa
     3001 acgttatctt tgtgcaactt ttccttaact ttataaagta atcgctttct tttgtctgtt
     3061 ttattctaac ttttatgata tactgttttc atgacagatt tatcaaaaca attacttgaa
     3121 aaagctcatg gtgggttaaa aataaatccg gatgagcaaa gacgctatct tggtactttt
     3181 gaggaaagag ttcttggata tgtagatatt gacacagcaa atagccctca gttagaaaaa
     3241 ggctttttat ttattttaga aaaccttcag gaaaaagcag agccactatt tgtgaagatt
     3301 tcaccaacta tcgaatttga taagcaagtt ttctacttaa aagaagcaaa agaaactgat
     3361 agtcaagcca ccatagtatc tgaagagcat attacttctc cttttggcct ggttattcat
     3421 agcaatgcac cagttcaagt agaagaaaaa gaccttcgac ttgcttttcc aaaactttgg
     3481 gaagttaaaa aggaagaacc agccaaaaca tccttatgga agaaatggtt tagctaaatc
     3541 ttgcacatat ttaataagtg cccaatattg gcagccgtgc gctccagata gaaactggca
     3601 tttttcaaac tatcttctaa aggttcactt ttctccaaaa tagaaaagac agcttggata
     3661 ttttcaaatg gtaggggagg taaatcttca gcaagactac cgcaaatagc aataacagga
     3721 actccaacag gggttctttt tgcaacacct ataggcgctt tcccagcaaa gctttgacta
     3781 tcaagtcttc cttctccaac aacaaccaag tcagcatctg aaactttctt atcaaagttg
     3841 attaagtcca agcaggtatc aattccagac acgatacttg cctgagcaaa ggcacacaaa
     3901 ccaccagcaa ggcctccacc tgctcctgct cctttaattt ctaatgttgc aggtgagaat
     3961 ttttcataaa aatcttggat cgcctgatct acgactgcaa acatagtcgg atgtagacct
     4021 ttttgattgc caaaagtgta agtcgcacct tgatgaccac ataagggact cacgacatct
     4081 gctaaaatat gaatttgaac accttcagga attttatagc aattttctgt tgaaacagaa
     4141 gctaagttta ataaggattg accggaagca ggcaagacat ttccatccct atcataaaat
     4201 tgataaccta aaccagcagc aatccccagt cctccatcat tactggccgt gccaccaaca
     4261 ccgatataaa tatctttaat ccctttagag atgagatgaa gaatcaactc tccaatacca
     4321 caagtttgga tttgaagtgg atttcgtttc tctagcggaa tttttccaag accaaccaag
     4381 tcagctactt caaatagtgc cagttcccct ttttgaaaat agcgcatggc ttctttttgt
     4441 ccaaaagggt ctgtcacttg gatccatttt tcttttaggt caagagaatg tcggatagca
     4501 tctacagtac cttctccccc atcaccaaca gggcagagga gacattctac atctgctatc
     4561 gattgttgga agcctctttt tattgcttca gctacctgtt gagctgtcaa gctttcctta
     4621 aacgaatccg gtgcaattac aatcttcata ttttccctca ttctaaacag tcaatcaaag
     4681 ggagaacttc taaaaaatcc ctcttgtcaa catgatgtgg tatttctttt ttgagcactt
     4741 ctttggcaca aaaggcgatt cctaacttcg ccgacttcaa cattaataga ttattaaccc
     4801 catcaccgat tgccaccgtt ctttctttag aaagttttag tttctttctc catttttcca
     4861 gagtctcttt tttgacctgg ggacttataa tttgtccaac taattttcct gttaaaagac
     4921 cttctttgac ttcaagctag ttggcagtga aataggcaat accaagggat tttgctaatc
     4981 tctccaacta ttggtgtaaa tccaccagac accagaccaa ctaggatgcc attcttttgg
     5041 agaatagaga tgaactctgg gacatttagc gatagatgaa ttgagttgaa gacgttatca
     5101 aagaccaaaa taggaagacc ttccaacaag gacactcttt ttcttaaact gctttcaaag
     5161 accaactctc ctcgcattgc tcgacttgta atctgcgaaa tttccgcctc atgacctgcc
     5221 tctctcccta aaagatcaat cacttcttct aggattaagg ttccatctac atccaaaaca
     5281 cacaagcctt ttacttgaga catcagttct cctctctaaa cagcctaaaa atcgtatgaa
     5341 gtcatcatac gattttatct attaattaac taaactatgg tacaagtcaa ggtatgactt
     5401 gcaggctgta tcccatgaga agtcactctc catagcttgt ttttgtaggt ttctccaaat
     5461 gtctggatgg tttctataca agtccaatgc tgtttggaaa gtccaattta accaataagg
     5521 agatagattg tcaaagctaa agccagtacc gcttccttcg attggattga aagcgcgaac
     5581 tgtatctcgc aagcctccaa cttcatggac caatggcaag gttccataac gcatagccat
     5641 catttgagac aagccacacg gttcaaaacg acttggcatg aggaagaggt cacaagcagc
     5701 gtagatttcc tgagcaagtt tgacatcaaa agtgatattt gttgatagct tgtctgggta
     5761 aatctgagca aaccatgaga aagctccttc aaaggctgga tcgccagttc ccaaaagaac
     5821 aatctgaaca tcttcttgca agatatggtg aagactttcg accaccacat caaaaccttt
     5881 ttgacgtgtc aaacgagaaa caattcccac cagtggaacg tctgctctaa caggcaagcc
     5941 aactctttct tgcaattttg ccttattttt ggctttccca gacaaatctt cctgattgaa
     6001 atgatagtct aaaagagcat ccgtctgagg attataaaga tcagcatcaa tcccattcac
     6061 gataccagat actttaccag actccatttt aagaatctga tccaaattac atccaaactg
     6121 actagtcata atttcatgag catagctagg tgaaacggtt gaaacacggt tcgcatagag
     6181 aatacctgcc ttcatccagt tcagacagtt gttccatcga agggtgccat cagcgtaacg
     6241 ttcaaagcca actccaaaca aatcacccaa cattccttct gaaaattgtc cttggaattc
     6301 taaattatga atggttaaaa ctgtttcaat gtcctcatag gcttgaatcc aacggtattt
     6361 ttccttcaac aagaaaggaa tcatagctgt atggtagtca tgaacatgga gaagatcagg
     6421 aataaagtca atcctttcca tagcctcaat ggcagccagt tggaaaaagg caaagcgttc
     6481 tccgtcatca aaatcaccgt aaacatgacc acggaagaaa taatattgat tgtcaataaa
     6541 gtagaaggtt acaccattta atactgtttt cttaattcca caatactgtc tgcgccaacc
     6601 aacgctcacc tcaaaatgaa gcacatcttc aatctgattt ccaaatttag cctctaccat
     6661 atcatagtag ggtaaaatca ctgcaacttc gtgcccagct tttaccagtg attttggaag
     6721 agcgccaatg acgtctccca aaccacctgt ttttgaaaag ggtgcaccct ctgctgctac
     6781 aaataaaatt ttcatgaatg aatatcctct gttactttag cacctttctt aaccacaact
     6841 ggatgttctg cagttcctcg aatcacaaca ccatgctcaa cttcaacccc tttgtccaag
     6901 atagcatatt cgacctgagc cccttctcca ataacaacac gagggaagag caggctatct
     6961 ttaaccaagc tatccttatg gacatgaata ttacgtgata gaacagaatt agctacttga
     7021 ccttcaataa tactaccaga ggcaaactga gaagtgctta ccttagatgt attagcatag
     7081 taagttggct cttcgttttt gacctttgta taaatctttt ggtttggtga gaaaagagaa
     7141 tagaattttt gtgattcaag catatcgata ttcgcttgat aataagattt aacagagtga
     7201 atattggcta gatagcccgt gtactcgtag gcgaaagctc cctcttttac agccaaatcc
     7261 cgtaaaacat agcgcaattt ctctggatgt tcttttttag cttcttcttc caagtgttca
     7321 atcaaccaag gtgtatcaac gacaaagata tctgtagaca tattgaacgt ttcagctgtt
     7381 gacttgctat caaagagttt atgagaaaga acatggtctg tttcatctac atccaagatt
     7441 gcatttactt ctgaaatatc tttcttagct agttttttat aaactacagt gataggctct
     7501 tttgttgtac tatgtaggtg gaaaacttgg ttcaaatcaa tgttaataag aacatcgcag
     7561 ttgagggcaa ccgtttggtt tgagccagaa cgtttcaaat aagtaagaag ctgttggtag
     7621 tattcttttc caactgtact actttctaca cgggtattgt aaattcctag atagtaatgg
     7681 ctaagaaggg ttgataagcc ccactcgcgt cctgaacgaa tatggtcaaa tactgagctg
     7741 atattatcct gctggaaaat accaaagaca ctacgaacac ctgcattagc aaggcttgaa
     7801 agtgggaagt caatcaaacg atatttccca ccaaatggca aacttgctac tggacggtgg
     7861 tccgtcaatg tcgacatatt gtgaaaacca actgtatttc ctaaaatggc agaatattta
     7921 tcaatcttca tctgttgcta cccccactac ttcattatat cctacaactt gtacttcatc
     7981 tgttccatca atttcgacac cgtcagaaat aatcgcacct tcaccaataa tggcacgttt
     8041 aatcttagct ccttgaccaa tgatagctcc actcatgata actgaatcaa ggacttccgc
     8101 tccttcgcga acttgcgcgc ctgttgaaag gatagaatgt ttaacagttc catcaacgaa
     8161 acatccgtct acaactaatg agtcttccac atgagcattt gccccgagga agtttggtgg
     8221 tgaaatcaag tttcttgagt aaatcttcca ttgacggtta cgactatcca aggcattttc
     8281 tggagaaata tactccatgt tcgcttccca aagtgactca atagtaccaa catctttcca
     8341 ataaccacta aattcgtaag cataaacact ttcacctgac tcaaggtaat ttggaatgac
     8401 atttttacca aagtctgaca tgccaacctt gctcttttca gcagcgacta acatattacg
     8461 aaggcgttgc caatcaaaaa tgtagattcc catagaagct tttgtagatt taggttgagc
     8521 tggtttttct tcaaattcaa caatacgatt gttagcatct gtgtttatga taccaaaacg
     8581 gcttgcttct ttaagaggga cgtctaaaac tgctactgtc aagctggcat tattatcctt
     8641 atgagactgg agcatatcat catagtccat tttgtagatg tgatccccag acaaaatcaa
     8701 gacatactca ggattgacac tgtcgatata gtcgatattt tggtaaatag cgtgactagt
     8761 cccctcaaac caacgatttc cttcacttgc agaataaggt tgaagaatag agacacctga
     8821 attaataccg tctagtcccc agcttgaacc attcccaata tggttgttga gagcaagtgg
     8881 ttgatactgt gtaacgaccc caacattgtg aatccctgag ttggcacagt ttgatagggc
     8941 aaagtcaatg atacggtagc gcccaccaaa ttgcacagct ggttttgcga tgctttgagt
     9001 gagtttaccg agacgagttc cttgcccacc agcaagaatc aaagctaaca tttcattttt
     9061 cattttctac tcctttttgg tttttatttg tgacggtttt agtagatttc aagcgacgtt
     9121 tgattttcca tacacttgct cccatagccg gtagggtaaa ggttaaggtc tgctcataat
     9181 ctttccatag tccttcttgc gtttgaacag tttgattatg ttctttccaa acgcctcccc
     9241 actcttccaa ctcagtattc catacttctt cgtaaattcc tgcaacgggt agtccgattg
     9301 taaaatcttt ccgctcaaca ggtaccatat taaagataca gactaacatt tctccctttt
     9361 tacccttacg aataaaggaa agaacactct ggtctcgatt atccgcatca atgatttcaa
     9421 taccatcata gctggtatca atttcccaca gacagcgatg atctttgtaa aactggttta
     9481 gctgagaagc gaaatacttc atcttagcat tcattgggtc ttctaggtta gaccattcca
     9541 actgttcttc agatttccat tctaggaatt gaccgtattc gctacccatg aagagcaatt
     9601 tcttaccagg gtgacaaatt tggtacgtat agagattgcg caagcctgcg aattgattgt
     9661 aacgatctcc ccacatctta tgcatcatac tcttcttgcc atgaaccact tcatcgtgcg
     9721 agaatggcaa gagataattc tccttgaaaa catacataaa gctgaaagtc accaggttaa
     9781 agtcatattt acgatagatc ggatcttctt cgtagaaacg gaggatatca ttcatccagc
     9841 ccatgttcca tttgtagtca aatcctagac caccaatctc tttcattccc gtaatcttga
     9901 tcgcagacga actttcttct gcaatcatca tcacatctgg atattctaac ttaataacct
     9961 cattcaagcg ctgaaggaaa taataacctt catagttgag atttccgcca tctttattag
    10021 gtgtccatgg agcatcatca tagtccaaat agagcatgtt gctaacagca tccacacgaa
    10081 taccatccaa atgatagaca tcaatccaat gcttaatgca agaaattaag aaggactgga
    10141 cttcattttt tccaaggtca aaattaaggg caccccaacc atggttatga gccttattat
    10201 ggtcttggta ttcaaaagtc ggtgtcccat cataataggc taaggcatca tcgttgatgg
    10261 taaagtgacc tggtacccag tccacaataa ccccaatatt atgggtatga cactcctcga
    10321 caaaatcttg aaactcctct ggtcggccat aagcatgctc taaagcgaag taacccataa
    10381 gctgataccc ccaactcaag cccaaaggat gggacatcaa gggcataaac tcaatatgag
    10441 tatagttcat ttcaacgaga taaggaatga gttcatcctt gagctgggca aaactataag
    10501 gactgccatc agaatttctt ttccatgatc cagcgtgaac ttcataaata ttgacaggac
    10561 gctcttcaaa gccccaacgt tttcttcgtg ccagccaaag tccatccttc catttcttct
    10621 caggaagctc tgttacgatt gcccctgttc ctggacgagc ctcatacctg acagcaaaag
    10681 ggtcaatctt catcagttga tgaccatttt gacgtgtgac atgatatttg taaatatgcc
    10741 cttcttgagc catattggta aagacttccc agaccccaaa atcatttctt accattggaa
    10801 tctgattttc aatccagttg gtaaaatcac caaccaagtg aacagcctga gcattaggtg
    10861 cccaaacacg gaaggtatag ccatgctctc catttagttc ttccctatgt gctcctagat
    10921 aatgttggag ataaaaattt tcacccgtca taaaggtttt taatgcttct ctattatcca
    10981 tatactcccc ttctcctgta agcgttttct atgtttttat tatactacct ttttagagaa
//
LOCUS       DAVHEW010000069        11038 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVHEW010000069 DAVHEW010000000
VERSION     DAVHEW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.069"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            395..871
                     /locus_tag="VVL53_001570"
     CDS             395..871
                     /locus_tag="VVL53_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4183722.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            909..1214
                     /locus_tag="VVL53_001571"
     CDS             909..1214
                     /locus_tag="VVL53_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4183723.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            1262..2737
                     /locus_tag="VVL53_001572"
     CDS             1262..2737
                     /locus_tag="VVL53_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU4183724.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            3042..9743
                     /locus_tag="VVL53_001573"
     CDS             3042..9743
                     /locus_tag="VVL53_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU4183725.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIENRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLYKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDYSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9991..10847)
                     /locus_tag="VVL53_001574"
                     /pseudo
     CDS             complement(9991..10847)
                     /locus_tag="VVL53_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3505 a         2103 c         2365 g         3065 t
ORIGIN      
        1 tttaccatgg actaaagttg tacaagaaaa gtgcaaataa gaaatctcca gattaggaac
       61 tatccgtgag ttcactaatc tggagatttt tcaatagact tcgttattgg gcggttacga
      121 tatgatcact gacttcgtca gtcttatcta caacctcaaa actgtgtttt gagcaacctg
      181 cggctagctt cctagtttac tctttgattt tcattgagta ttagaacaga aaaaatgctt
      241 ggagtattta tttgtgtgtt tatttttata taacaaacta taaacaaaac aaaaatataa
      301 aaaaagagac aaaaaagaac agaaagtaat tgacaacggt ttcatatagt gttatactta
      361 tggcataaag ttaatggaaa gaagggaaaa acttatggta ttagattatt tctttgacaa
      421 aaaccttgtg ttttgcttag aagcggataa tcaagaacaa ctctttgatc aggttgcaac
      481 tttattggaa gaacgagaaa ttgtcactcc aacttatcgt gaagctttga ttacgcgtga
      541 aaagtcattt ccaactggtt tagatatgga atttctagga aaggacttgc caaatgtagc
      601 gattcctcat acagatattg ttcataatct agctgagaaa gtggtggttg ttcgattaga
      661 aaaaccagta acttttcaca atatgatagc tcctgataag gaagtagaag tatccttgct
      721 cttctttatc atcaataact caagttcaag tcaaacaaat attctggctc agttgatgga
      781 ctttttcaca ggaaatggac atcttgaaga cctatcaaaa atttccgaac cagaaaaact
      841 ttatgcttac attgctgaaa caaccgctta atcttgtcta ttaaaaaata aaatcggagg
      901 aaatctaaat gattaaaatt cttgctgcct gcggtgcagg tgttaactca agtcaccaaa
      961 ttaaaagtgc tctagaagaa gaactttcaa accgtggtta tgatgttcac tgtgatgcag
     1021 tcatggtgaa agatgtaaac gaagacctta tgaaaggtta cgatatcttt acaccaatcg
     1081 ctgcaacaga ccttggtttt gaaccaggta tcccagttat cgaagctgga ccaatcttat
     1141 tccgtatccc agcaatgagc gctccagtat ttgacaatat tgaagcagct attaaagaac
     1201 acggattaag ctaattctaa tttgttaaca ttcatataac ataaaaaagg gaggaactgt
     1261 tatggatgca atctttgacc taatcggaaa ggttttcaat cccatcttag aaatgggtgg
     1321 acctgtcatc atgttaatca ttttgacagt attggcttta ctttttggag tgaaattctc
     1381 caaagcgctt gaaggtggta tcaaacttgc catcgctctt acaggtatcg gtgctatcat
     1441 cggtatgcta aatggtgctt tctcagcatc acttgcaaaa ttcgttgaaa acactggtat
     1501 ccaattgagt attaccgacg ttggttgggc accacttgca acaatcactt ggggttctgc
     1561 ttggacactt tacttcttgc tcatcatgtt gattgtcaac gtagtgatgc ttgctatgaa
     1621 gaaaaccgat acacttgatg tcgatatctt tgatatctgg cacttgtcta tcacaggtct
     1681 cttgattaaa tggtatgctg ataacaatgg tgtgagtcaa ggggtttcac tcttgattgc
     1741 tacagcagct atcgttcttg tcggtgtgtt gaaaattatc aactctgact tgatgaaacc
     1801 tacatttgat gaccttctta acgccccaag ttcatcacca atgacatcaa ctcacatgaa
     1861 ctacatgatg aacccagtta tcatggtttt ggataagatt tttgaaaaat tcttcccagg
     1921 ccttgataaa tatgactttg atgctgctaa attgaacaag aaaatcggtt tctggggatc
     1981 taaattcttc atcggtttca tccttggtat cgttatcggt attatgggaa ctccacatcc
     2041 aattgcaggt gttgcagatg cagataaatg gcgtcttgtt atcaaaggat ggttgtctct
     2101 tggtttgact gccggtgtat ctttggaact cttctcactt atcggttcat ggttcatcgc
     2161 agccgtagaa ccactatcac aaggtattac aaacgttgct actaaacgtc ttcaaggacg
     2221 taaattcaat atcggtcttg actggccatt catcgctggt cgtgctgaaa tctgggcttg
     2281 tgccaacgta cttgcaccaa tcatgttgat tgaagcagtg cttctttcaa aagttggaaa
     2341 tggtatcttg ccacttgcag gtatcatcgc tatgggtgtt actccagctc tcttggttgt
     2401 aactcgtggt aaattgctcc gtatgattat cttcggaaca ctcttgttgc cactcttcct
     2461 tctttcaggt acacttattg caccatttgc aacagaactt gctaaaggtg taggtgcctt
     2521 cccagaaggt gtgagccaaa ctcaattgat tactcactct actcttgaag gaccaatcga
     2581 aaaacttctt ggttggacaa ttggtaacac tacaactggt gatatcaaag caatccttgg
     2641 tgcagtagcc ttccttgtat tctatatcgg tatctttgct tggtacagaa aacaaatgat
     2701 caaacgtaac gaagagtacg cagcaaaagc aaaataatgc gctcctataa aatataaact
     2761 caatgaaaat caaagagcaa actaggaagc tagccgcagg ttgctaaaaa cacagttttg
     2821 aggttgtaga taagactgac gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa
     2881 ctgacgaagt cagtaatcat acatacggca aggcgacact gacgaagttt gaattggatt
     2941 ttcgcagagt gtaaattgtt gaaactaggt tgtcatacca tgtataccac ttgggctttg
     3001 gcagtagcta actgcactaa atataatata aggagggttt tatggggaaa ggccattgga
     3061 atcggaaaag agtttatagc attcgtaagt ttgctgtggg agcttgctca gtaatgattg
     3121 ggacttgtgc agttctatta ggaggaaata tagctggaga atctgtagtt tatgcggatg
     3181 aaacacttat tactcatact gctgagaaac ctaaagagga aaaaatgata gtagaagaaa
     3241 aggctgataa agctttggaa actaaaaatg tagttgaaag gacagaacaa agtgaaccta
     3301 gttcaactga ggctattgca tctgagaaga aagaagatga agccgtaact ccaaaagagg
     3361 aaaaagtgtc tgctaaaccg gaagaaaaag ctccaaggat agaatcacaa gcttcaagtc
     3421 aagaaaaacc gctcaaggaa gatgctaaag ctgtaacaaa tgaagaagtg aatcaaatga
     3481 ttgaaaacag gaaagtggat tttaatcaaa attggtactt taaactcaat gcaaattcta
     3541 aggaagccat taaacctgat gcagacgtat ctacgtggaa aaaattagat ttaccgtatg
     3601 actggagtat ctttaacgat ttcgatcatg aatctcctgc acaaaatgaa ggtggacagc
     3661 tcaacggtgg ggaagcttgg tatcgcaaga ctttcaaact agatgaaaaa gacctcaaga
     3721 aaaatgttcg ccttactttt gatggcgtct acatggattc tcaagtttat gtcaatggtc
     3781 agttagtggg gcattatcca aatggttata accagttctc atacgatatc accaaatacc
     3841 tttacaaaga tggtcgtgag aatgtgattg ctgtccatgc agtcaacaaa cagccaagta
     3901 gccgttggta ttcaggaagt ggtatctatc gtgatgtgac tttacaagtg acagataagg
     3961 tgcatgttga gaaaaatggg acaactattt taacaccaaa acttgaagaa caacaacatg
     4021 gcaaggttga aactcatgtg accagcaaaa tcgtcaatac ggacgacaaa gaccatgaac
     4081 ttgtagccga atatcaaatc gttgaacgag gtggtcatgc tgtaacaggc ttagttcgta
     4141 cagcgagtcg taccttaaaa gcacatgaat caacaagcct agatgcgatt ttagaagttg
     4201 aaagaccaaa actctggacc gttttaaatg acaaacctgc cttgtacgaa ttgattacgc
     4261 gtgtttaccg tgacggtcaa ttggttgatg ctaagaagga tttgtttggt taccgttact
     4321 atcactggac tccaaatgaa ggtttctctt tgaatggtga acgtattaaa ttccatggag
     4381 tatccttgca ccacgaccat ggggcgcttg gagcagaaga aaactataaa gcagaatatc
     4441 gccgtctcaa acaaatgaag gagatgggag ttaactccat ccgtacaacc cacaaccctg
     4501 ctagtgagca aaccttgcaa atcgcagcag aactaggttt actcgttcag gaagaggcct
     4561 ttgatacttg gtatggtggc aagaaacctt atgactatgg acgtttcttt gaaaaagatg
     4621 ccactcaccc agaagctcga aaaggtgaaa aatggtctga ttttgaccta cgtaccatgg
     4681 tcgaaagagg caaaaacaac cctgctatct tcatgtggtc aattggtaat gaaataggtg
     4741 aagctaatgg tgatgcccac tctttagcaa ctgttaaacg tttggtcaag gttatcaagg
     4801 atgttgataa gactcgctat gttaccatgg gagcagataa attccgtttc ggtaatggta
     4861 gcggagggca tgagaaaatt gctgatgaac tcgatgctgt tggatttaac tattctgaag
     4921 ataattacaa agcccttaga gctaagcatc caaaatggtt gatttacggt tcagaaacat
     4981 catcagcaac ccgtacacga ggaagttact atcgccctga acgtgaattg aaacatagca
     5041 atggacctga gcgtaattat gaacagtcag attatggaaa tgatcgtgtg ggttggggga
     5101 aaacagcaac cgcttcatgg acttttgacc gtgacaacgc tggctatgct ggacagttta
     5161 tctggacagg tacggactat attggtgaac ctacaccatg gcacaaccaa aatcaaactc
     5221 ccgttaagag ctcttacttt ggtatcgtag atacagccgg cattccaaaa catgacttct
     5281 atctctacca aagccaatgg gtttctgtta agaagaaacc gatggtacac cttcttcctc
     5341 actggaactg ggaaaacaaa gaattagcat ccaaagtagc tgactcagaa ggtaagattc
     5401 cagttcgtgc ttattcgaat gcttctagtg tagaattgtt cttgaatgga aaatctcttg
     5461 gtcttaagac tttcaataaa aaacaaacca gcgatgggcg gacttaccaa gaaggtgcaa
     5521 atgctaatga actttatctt gaatggaaag ttgcctatca accaggtacc ttggaagcaa
     5581 ttgctcgtga tgaatctggc aaggaaattg ctcgagataa gattacgact gctggtaagc
     5641 cagcggcagt tcgtcttatt aaggaagatc atgcgattgc agcagatgga aaagacttga
     5701 cttacatcta ctatgaaatt gttgacagcc aggggaatgt ggttccaact gctaataatc
     5761 tggttcgctt ccaattgcat ggtcaaggtc aactggtcgg tgtagataac ggagaacaag
     5821 ccagccgtga acgctataag gcgcaagcag atggttcttg gattcgtaaa gcatttaatg
     5881 gtaaaggtgt tgccattgtc aaatcaactg aacaagcagg gaaattcacc cttactgccc
     5941 actctgatct cttgaaatcg aaccaagtca ctgtctttac tggtaagaaa gaaggacaag
     6001 agaagactgt tttggggaca gaagtgccaa aagtacagac cattattgga gaggcacctg
     6061 aaatgcctac cactgttccg tttgtataca gtgatggtag ccgtgcagaa cgtcctgtaa
     6121 cctggtcttc agtagatgtg agcaagcctg gtattgtaac ggtgaaaggt atggctgacg
     6181 gacgagaagt agaagctcgt gtagaagtga ttgctcttaa atcagagcta ccagttgtga
     6241 aacgtattgc tccaaatact gacttgaatt ctgtagacaa atctgtttcc tatgttttga
     6301 ctgatggaag tgtacaagag tatgaagtgg acaagtggga gattgccgaa gaagataaag
     6361 ctaagttagc aattccaggt tctcgtattc aagcgaccgg ttatttagaa ggtcaaccaa
     6421 ttcatgcaac ccttgtggta gaagaaggca atcctgcagc acctgtagtg ccaactgtta
     6481 ctgttggagg tgaagcagta acaggtctta ctagtcgaca accaatgcaa tatcgtactc
     6541 tatcttatgg tgcccaattg ccagaagtca cagcaagtgc taaaaatgca gctgttacag
     6601 ttcttcaagc aagcgcagca aacggcatgc gtgcgagcat ctttattcag cctaaagatg
     6661 gtggccctct tcaaacctat gcaattcaat tccttgaaga agcgccaaaa attgctcact
     6721 tgagcttgca agtggaaaaa gctgacagtc tcaaagaaga ccaaactgtc aaattgtcgg
     6781 ttcgagctca ctatcaagat ggaacgcaag ctgtattacc agctgataaa gtaaccttct
     6841 ctacaagtgg tgaaggggaa gtcgcaattc gtaaaggaat gcttgagttg cataagccag
     6901 gagcagtcac tctgaacgct gaatatgagg gagctaaagg ccaagttgaa ctcactatcc
     6961 aagccaatac tgagaagaag attgcgcaat ccatccgtcc tgtaaatgta gtgacagatt
     7021 tgcatcagga accaagtctt ccagcaacag taacagttga gtatgacaaa ggtttcccta
     7081 aaactcataa agtcacttgg caagctattc cgaaagaaaa actagactcc tatcaaacat
     7141 ttgaagtact aggtaaagtt gaaggaattg accttgaagc gcgtgcaaaa gtctctgtag
     7201 aaggtatcgt ttcagttgaa gaagtcagtg tgacaactcc aatcgcagaa gcaccacaat
     7261 taccagaaag tgttcggaca tatgattcaa atggtcacgt ttcatcagct aaggttgcat
     7321 gggatgcgat tcgtccagag caatacgcta aggaaggtgt ctttacagtt aatggtcgct
     7381 tagaaggtac tcaattaaca actaaacttc atgttcgcgt atctgctcaa actgagcaag
     7441 gtgcaaacat ttctgaccaa tggaccggtt cagaattgcc acttgccttt gcttcagact
     7501 caaatccaag cgacccagtt tcaaatgtta atgacaagct catttcctac aataaccaac
     7561 cagccaatcg ttggacaaac tggaatcgta ctaatccaga agcttcagtc ggtgtcctat
     7621 tcggagattc aggtattttg agcaaacgct ccgttgataa tctaagtgtc ggattccacg
     7681 aagaccatgg agttggtgca ccgaagtctt atgtgattga gtattatgtt ggtaagactg
     7741 tcccaacagc tcctaaaaac cctagttttg ttggtaatga ggaccatgtc tttaatgatt
     7801 ctgccaactg gaaaccagtt actaatctaa aagcccctgc tcaactcaag gctggagaaa
     7861 tgaaccactt tagctttgat aaagttgaaa cctatgctgt tcgtattcgc atggttaaag
     7921 cagataacaa gcgtggaacg tctatcacag aggtacaaat ctttgcgaaa caagttgcgg
     7981 cagccaagca aggacaaaca agaatccaag ttgacggcaa agacttagca aacttcaacc
     8041 ctgatttgac agactactac cttgagtctg tagatggaaa agttccggca gtcacagcaa
     8101 gtgttagcaa caatggtctc gctaccgtcg ttccaagcgt tcgtgaaggt gagccagttc
     8161 gtgtcatcgc gaaagctgaa aatggcgaca tcttaggaga ataccgtctg cacttcacta
     8221 aggataagag cttactttct cataaaccag ttgctgcggt taaacaagct cgcttgctac
     8281 aagtaggtca agcacttgaa ttgccgacta aggttccagt ttacttcaca ggtaaagacg
     8341 gctacgaaac aaaagacctg acagttgaat gggaagaagt tccagcggaa aatctgacaa
     8401 aagcaggtca atttactgtt cgaggccgtg tccttggtag taaccttgtt gctgaggtca
     8461 ctgtacgagt gacagacaaa cttggtgaga ctctttcaga taaccctaac tatgatgaaa
     8521 acagtaacca ggcctttgct tcagcaacca atgatattga caaaaactct catgaccgcg
     8581 ttgactatct caatgacgga gattattcag aaaatcgtcg ttggacaaac tggtcaccaa
     8641 caccatcttc taatccagaa gtatcagcgg gtgtgatttt ccgtgaaaat ggtaagattg
     8701 tagaacggac tgttgcacaa ggaaaagttc agttctttgc agatagtggt acggatgcac
     8761 catctaaact cgttttagaa cgctatgtcg gtccagagtt tgaagtgcca acctactatt
     8821 caaactacca agcctacgac gcagaccatc cattcaacaa tccagaaaat tgggaagctg
     8881 ttccttatcg tgcggataaa gacattgcag ctggtgatga aatcaacgta acatttaaag
     8941 ctatcaaagc caaagctatg agatggcgta tggagcgtaa agcagataag agcggtgttg
     9001 cgatgattga gatgaccttc cttgcaccaa gtgaattgcc tcaagaaagc actcaatcaa
     9061 agattcttgt agatggaaaa gaacttgctg atttcgctga aaatcgtcaa gactatcaaa
     9121 ttacctataa aggtcaacgg ccaaaagtct cagttgaaga aaacaatcaa gtagcttcaa
     9181 ctgtggtaga tagtggagaa gatagctttc cagtacttgt tcgcctcgtt tcagaaagtg
     9241 gaaaacaagt caaggaatac cgtatccact tgactaagga aaaaccagtt tctgagaaga
     9301 cagttgctgc tgtacaagaa gatcttccaa aaatcgaatt tgttgaaaaa gatttggcat
     9361 acaagacagt tgagaaaaaa gattcaacac tgtatctagg tgaaactcgt gtagaacaag
     9421 aaggaaaagt tggaaaagaa cgtatcttta cagcgattaa tcctgatgga agtaaggaag
     9481 aaaaactccg tgaagtggta gaagttccga cagaccgcat cgtcttggtt ggaaccaaac
     9541 cagtagctca agaagctaaa aaaccacaag tgtcagaaaa agcagataca aaaccaattg
     9601 attcaagtga agctagtcaa actaataaag cccagttacc aaatacaggt agtgcggcaa
     9661 gccaagcagc agtagcagca ggtttagctc ttctaggttt gagtgcagga ttagtagtta
     9721 ctaaaggtaa aaaagaagac tagaaatttt gataccttct ttatcattaa agaccaaaga
     9781 aaaactcttg tcctaaatag aagataaaga gtctggaaca aaatagttct caaccacaaa
     9841 aaagctagat atttccaatt gcggaactct agctttttaa ttttgagtcg ttctcttatt
     9901 gtatagtaga ttgaaactag agtagtacac ttctacttct aaaacattgt tagaaatcga
     9961 tttgactgtc cttatcgatt tgtcctgttc ttatttcatt tcactatgtt ttaggaggtt
    10021 attggccata agtaccaatc ccatgtcaat tctcacttga cgcttgcccc tcagattaca
    10081 tctcttatga cccaaacaag cctttatctg cccaaagaca ggtttcacat caatcttgcg
    10141 ttgagcgaaa atctgtctac cttggggaga taaaagcgcc tgacattctt tagctttcaa
    10201 gttttgatag cgttcgttca tatacagtcc tttttgaggg gctgattcag gttcatcagc
    10261 gtagtaaacc ttgatttcct gttgaaagtc tgtctgtgtt ttctggtgct tgatatggtg
    10321 aaaacgatag caccagccat caggatgtat gtagctatcc tccttgtcat tatagtgcca
    10381 attcgctcag tttttagctg actctttata ccctctcttt tgtttcttat caaacatggc
    10441 atatttaatc agaagggaag tgatttcttc ttgaaaatag acttgtatct gttcttgaag
    10501 tttggcggag aatttctctg tcgttttctt ccacacaaaa ctatacttgt tggcattggc
    10561 ttcaatctta gttccgtcaa taaacaaaca atctaaggtc actaactctt ccatttttca
    10621 atttttcgac cagagaaaat cccttgtgaa taggcaaata gaagagtaga tacaagcatt
    10681 ttagggtgat aagacgggcg atcaaaggca tgataggagg tgtagaagtg acgttcctcc
    10741 aaggtattca ccactttttc aatagtaaag acgagatggt cttgtggtaa gaaggaactg
    10801 atttctagtg gtaaagttgt ttgatttgtg ttatagtgaa tatgcatggg atagcctttt
    10861 taaatggttt attaagcaat tctattttac caagactatc gtaaccatgc aaaaaaagca
    10921 acggcaaatc cgttgctttt tttggagata agagactatt gtcccaggct cttttctttg
    10981 tataaaattg tatagtgtta ttgtctatac tattttgtag agaaagcaaa aatcccta
//
LOCUS       DAVHEW010000070        10993 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVHEW010000070 DAVHEW010000000
VERSION     DAVHEW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.070"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            11..733
                     /locus_tag="VVL53_001575"
     CDS             11..733
                     /locus_tag="VVL53_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU4183726.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VVL53_001576"
     CDS             730..1578
                     /locus_tag="VVL53_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU4183727.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VVL53_001577"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VVL53_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU4183728.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VVL53_001578"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VVL53_001578"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU4183729.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VVL53_001579"
     CDS             complement(3249..5957)
                     /locus_tag="VVL53_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4183730.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALTTHLQDQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSFILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VVL53_001580"
     CDS             complement(5959..6660)
                     /locus_tag="VVL53_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183731.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VVL53_001581"
     CDS             complement(6910..9321)
                     /locus_tag="VVL53_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU4183732.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYGFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFNQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDYPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQLNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARAGLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKISTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9374..10066)
                     /locus_tag="VVL53_001582"
     CDS             complement(9374..10066)
                     /locus_tag="VVL53_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU4183733.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10601..10981)
                     /locus_tag="VVL53_001583"
     CDS             complement(10601..10981)
                     /locus_tag="VVL53_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183734.1"
                     /translation="MKLKRLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
BASE COUNT         3211 a         2522 c         2057 g         3203 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tataaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ccgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gcatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtt ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 cgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catatgagct ggcgtttttt
     2581 ctatgcccac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcac
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg aagcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctatcaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccaag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcgtcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaacaagc tcaagacaaa agctagtaag ctataggtcc aatagaactg aatctgagtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttggcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag ttgcagacgt
     5221 accttgccat tatctgataa aatttgttca agctcttcct gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ttgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggtct tgcaaatgag tggtcaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatctaa ggtttgagca gttgttaaat aagcattagc tgtcgcctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat ttgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tgtcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 gagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tattgagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagaactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagatccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct acaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttggaaactg cctatattct taaatcctta ttaaaaggaa gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatatt tttaactgct ctgccaataa
     7321 tttatgggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggccagca cgtgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 atgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataggtatt gccaatcgaa atcggcccat gttctgggga
     7741 ataagacgga gaagagaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctcc ctgagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttttcctcac ctttttgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgact ggaaagaccg tctctaggag
     8101 attggtaaca taggctggcc aataattcag ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggtagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgctcatact ttccatcaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttcca acccttcctt agtaaaacat tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcgc
     8881 aagtgcctta ctaatattca actgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggttg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaacc atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagcccct ataaggccaa atacttttgc tcctaaagaa ccattgccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga agctggttgc ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aagcctagaa atcaatccta
     9361 ggcttacaat tttttatttt ccaaggtagt attcagaaac aacgttcaat ttttcgtcga
     9421 attcgaatac caatggtggg aagttaggga tttccacgtc catgatctca tcatctgaca
     9481 aacctttgat gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt
     9541 ttttaccatc tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca
     9601 aagtcacttt caagttttca gcatctggga taactgagtc gtcaagtgaa gcgtaacgac
     9661 ggtctgtgtg agctgagtgc tcatcatcac ggtccatgtt tggaggcaat acatcgtatg
     9721 aacgacgcca gatgtgaact tgctcatcac caaattgttc agcagcttca gctttgtttt
     9781 taccagtcaa accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc
     9841 acaattggtc agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg
     9901 aagtgtaagc ttggtcaaat ttgataccag cttctttgat caatttacca gcgtcaatcg
     9961 cttgttgtgt acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt
    10021 tgttccattc agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc
    10081 tttaaatttt ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa
    10141 aaagctagtc aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt
    10201 tttggttgtt tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt
    10261 ttgagttgac tcttttagtt tagtaaacga gtcattattt tccatagcta tgcctttgaa
    10321 accttcctca gctttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc
    10381 tgcataagtg cgtattgatt caatcgcatc gtctgttaca accggtgtat cgaaagtgat
    10441 tgtttctttt gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatt
    10501 ctttgaactt tatgttccac atgcaactag aaaagaaaga gataatagag ctactcctat
    10561 accgataaac ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt
    10621 tcttcaatgt tgcaaggaat agagatcctc cgataatagc aagaatcccc ccaacccatc
    10681 ctaagaatgg aatgagacta actccaccag aaacaatcat tagtacagac ggagctgcac
    10741 ctacacgctt atctccttta tagtaaacaa tagcaatgat accaagaaca agatttgcaa
    10801 tcttcaaggc attcaagagt aaggcaacac ctgaaaaagc ccctgcagta gctcctccat
    10861 taagagttgt agcagctgtg tttaccgctg ttcccaacaa gacaaaagga ccaataagaa
    10921 gtaaaattcc tcccacaaga ccgacgatac cactaattaa agcaagtctt tttaatttca
    10981 taataaaatc tcc
//
LOCUS       DAVHEW010000071        10880 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVHEW010000071 DAVHEW010000000
VERSION     DAVHEW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.071"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..323)
                     /locus_tag="VVL53_001584"
     CDS             complement(<1..323)
                     /locus_tag="VVL53_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4183735.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIIN"
     gene            complement(463..987)
                     /locus_tag="VVL53_001585"
     CDS             complement(463..987)
                     /locus_tag="VVL53_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU4183736.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1040..1276)
                     /locus_tag="VVL53_001586"
     CDS             complement(1040..1276)
                     /locus_tag="VVL53_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU4183737.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(1344..2264)
                     /locus_tag="VVL53_001587"
     CDS             complement(1344..2264)
                     /locus_tag="VVL53_001587"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU4183738.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            2454..2857
                     /locus_tag="VVL53_001588"
                     /pseudo
     CDS             2454..2857
                     /locus_tag="VVL53_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3073..3654
                     /locus_tag="VVL53_001589"
     CDS             3073..3654
                     /locus_tag="VVL53_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4183739.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            3651..4928
                     /locus_tag="VVL53_001590"
     CDS             3651..4928
                     /locus_tag="VVL53_001590"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU4183740.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            4946..5608
                     /gene="deoC"
                     /locus_tag="VVL53_001591"
     CDS             4946..5608
                     /gene="deoC"
                     /locus_tag="VVL53_001591"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU4183741.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            5595..5984
                     /locus_tag="VVL53_001592"
     CDS             5595..5984
                     /locus_tag="VVL53_001592"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU4183742.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6074..7126
                     /locus_tag="VVL53_001593"
     CDS             6074..7126
                     /locus_tag="VVL53_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU4183743.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAERGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7270..8805
                     /locus_tag="VVL53_001594"
     CDS             7270..8805
                     /locus_tag="VVL53_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183744.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            8798..9856
                     /locus_tag="VVL53_001595"
     CDS             8798..9856
                     /locus_tag="VVL53_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183745.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            9859..10815
                     /locus_tag="VVL53_001596"
     CDS             9859..10815
                     /locus_tag="VVL53_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183746.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3096 a         1937 c         2477 g         3370 t
ORIGIN      
        1 tggttgatga tacgagggat ttggtgattt ttctttacca ggggagtctc agcaaccatc
       61 atttttgaac agtgatagca cttgaaacgg cgttttctaa ggagaattct agaaggcata
      121 ccagttgttt cgaggtaagg gatcttagac ggtttttgaa agtcatattt cttcattaga
      181 cttccacaat cagggcaaga tggagcctca taatccagct tagcgataat ttctttgtgg
      241 gtatccatat tgatgatatc tagaatcttg atgtttgggt ctttaatatc gagcagtttt
      301 gtgataaaat gtaattgttc catatgattc tttctaatga gttgttttgt cgcttttcat
      361 tataggtcat atgggacttt ttttctacac aaaaataggc tccataatat ctatagtgga
      421 tttacccact acaaatatta tagagcccaa aaaggaagcc ctttatgaat tgtaggactt
      481 ccttttctta tccagaaatt gatctagctc tctctgattt cgaagaatag tgactttatg
      541 tgaatattct tggcaaagtt tttggtaatt ttctttttga gttttgctac gcccatccca
      601 aagaatccat ctgataaact cccactcaaa gcgttcaggg caatctaccg ccatactttc
      661 tctgactttt ccacggtatt taagataacg cttaaaggct ctaaagagac aggtcaatgg
      721 cgaaaaattg agaaagatga tttggtcagc ttcttgcatt cgttcttggt agtagcacca
      781 agaataatta ccatcgatga cccaagcttt atgcttggtg agaaagtttt ttatctcggt
      841 taacatccat tcgcagtcac tgtcttgcca accaggttga aattggagtg tgtccatgtg
      901 cagttttgga atggagtagt agttagataa cttttctgct atagttgact taccagaacc
      961 agaatatccg ataattgcga ttttcatttt ctaccttttc ctatttggag acaaaaaaac
     1021 agcctctatg gactgtttct tatttaacaa gtttagctga aagacgagct ttatcgcggc
     1081 ttgctttgtt tttgtgaatc aaacctttag tttctgcttt atcgatagct gagctagcag
     1141 cacggaaaag ttcttcagat gggtttgctt cgaaagcttt tatagcagta cgcatagctg
     1201 atttttgagc tgagttcttt tcgttttgtt taacgttcaa ttcagcgcgt ttgatagctg
     1261 atttaatgtt tgccaatgtt cttacctcca tatttactaa ctataccatt atatatgaaa
     1321 acttacgttt tgacaaggga aaattacttt tttaagtaaa tttcatcgat ttcatggttc
     1381 ttgcttttat gaagaatcac ttctgcacga tttctggttg gttcaatata attttgcaga
     1441 tttgtgagat tgatactgat ccagacctga tgggcaaagg actccacttc cccaatcggc
     1501 atctgagtaa aacgataata gtagctatca gggtcgtttt gggctagact cagcattttc
     1561 aagaaacggt ccagatacca actttcaata tcatccactc cagcatctac atagatggaa
     1621 aagtcaaaga agtcagtgat atagagacga tcgttttgtg gattttgaaa gacattgatt
     1681 ccctcaacaa ttacaaaatc agcagctttg acactttgtt tctcttcggg tacgatgtcg
     1741 taaacttcat gagaatagac aggaatatct acatcttgtc catttttgat gcggtccaag
     1801 aagttgagaa gagcttccat atcatagctt tcaggaaatc ctttacgatt taaaatcccc
     1861 tgctcaatca aggtttgatt gggatagaga aaaccatcag ttgtaaccaa ctcaaccgta
     1921 gcatctgtaa acgtacggga cagtaggatt tgaagtaggc gactggttgt ggattttcca
     1981 acggcaacac tcccagaaac cccaataata aaaggttgag atttactttc acgttggagg
     2041 aaaattcctt ttgaaaaggc taaatcttcc ttagttcgct tgtaaatctg aatcaaatga
     2101 gccaagggga gatagatatc tgtaacgtct tggagactga tttggtcatt aaaactcttg
     2161 atagattcca attcttcttc tgtcaaagga ggtgttgtct ttcgatgtaa agattgccaa
     2221 gtctggcggc tgattttttc aaaatgtaaa aattcgttgg tcatttgttt tcccctagat
     2281 attttgtcta tcataccata aaaagctaaa aaaataaagc ggtttcttga atgtggtaag
     2341 tcaatggtat atagtataag tgatcaattt attgatgaaa taggagatag attctactga
     2401 tttataagct tataaagggg gctttttagt tcacaactta taggaggtgt actatgaaaa
     2461 tcttaaaacg ttacatattg gaactctgtt ttattttaag ttttgcttta ccttttataa
     2521 aaggaaccaa tgcagataat ggtagatgct ttgtggaaac ctattacggt tttacttttt
     2581 tgatggaaca tgctattgta acagctgtct ttatttgttc gttcttaatt gctttcttac
     2641 taaaaaacga tggacgaaat ggattgctgc gggtagttat tgctttttag tcctacggat
     2701 tgcgacagag ggttatttct tccacatgtc actagaagat ttgatacgac tttggacaag
     2761 tttggaaatc ctgacacaaa cgtatcagtt gggcttttat ctaaacattt tgttaggaat
     2821 tttgttgata ataaagtatt ttaaggttaa gcaatagaaa taataatctg cctgattata
     2881 gatgataaac tttttgtttt tatctggact gtgatattta tagttctaaa tggtttatta
     2941 tttataagtg ttgaaagaga ttgaagaaaa tggcttcgtg acttgctttc ttcggtcttt
     3001 ttcgttttat tatcaatttg taaacgattt acaatttgag tcaaattatg gtaaaatagt
     3061 gtcatgagta aaatgtacta tgcagaaaat cctgacgctg ctcacgacat tcatgagttg
     3121 agagtggact tgttgggaga aaaaatgact tttttgacag atgcgggtgt ttttagcaag
     3181 aaaatggttg actttggaag tcaactcttg ctcaagtgtc tggatgtcaa ccaaggagag
     3241 acagtcctag atgtaggctg tggttatggt ccattaggtt tgtccttggt caaggtttac
     3301 ggagttcagg cgaccatggt tgatattaac actcgtgccc tggatttagc tcagagaaat
     3361 gctgaaaaaa ataatgcaaa agcgacgata tttcaatcta atatttatgc aagagttcaa
     3421 gggcgatttg accatgtcat ttccaatcct cctatccgtg cgggcaagca agtcgttcat
     3481 gaaatcatcg agaaaagtaa agatttcttg gaaattggtg gagatttgac catcgtcatc
     3541 cagaaaaaac aaggagctcc aagtgccaag tctaagatgg aagatgtgtt tggcaattgt
     3601 gaaatcctta aaaaagacaa gggatactat atccttagaa gtgtgaaaga atgagagcag
     3661 ttgatttaat ccaaaaaaaa cgagacggtc aagaactgac ttcaagtgaa attgaatggc
     3721 tgatagaagg ctatgtatca ggaactgttc ctgattatca gatgtctgcc tttgctatgg
     3781 ctgtttattt taaaggaatg acgactcgag aaatctctga tttaacgatg aatatggtta
     3841 agactgggca agagtttgac ctgtcagcca ttgatggtgt gaaagttgac aagcattcta
     3901 ctggtggtgt cggcgataag gtaaccttga ttttagctcc tcttgttgct agcttcggtg
     3961 tgcctgtagc aaaaatgagt ggtcgtggtc ttggccatac aggcggaaca attgataaat
     4021 tggagtccat taagggctat caagtagaac gtagtcaaga ggattttatt cgtcaggttc
     4081 aggatattgg agtatctgtc attgggcaat cagaccagct ggtcaaagca gataagcttc
     4141 tctatgccct tcgtgatgtg accgcaactg tcgacacgat tcctttgatt gcgagttcgg
     4201 tgatgagcaa gaaaattgcg gcaggggcgg atgctatttt gctagacgtg actgtcggtg
     4261 agggtgcctt catgaagacg gttgatgagg cgcgtgagtt ggctcaaact atggtagatc
     4321 ttggtaaggt tgttggacgg aagacagtag cagtgattac ggatatgagc cagcctttgg
     4381 gacgagcgat tggaaatcgt ctggaaatcc ttgaagcatt ggagattttg caaggtcaag
     4441 gacgtcagga tattacccac tttatttgtg aattggctca aattatgctt ggtttggcaa
     4501 atgttaacaa aactgttgaa gaagttcacc aacatcttga gaatggtcaa gcactggcta
     4561 agtttgagga aatggtgcaa gcccaaggtg gagacttgga agacctctat cgtcctgtaa
     4621 atgtagctca tgtggtggaa atccctgctc aggaaacggg tgtcatttca gctcttccag
     4681 ctatggactt tggtctttat gccatgaggc tgggtgctgg tcgtgcagtt aagtctgatg
     4741 ccttggacta tgaaacagga attgtttttg aaaagaagat tggagactcc gttcaaaagg
     4801 gtgaaattgt tgcaaaagta tacacaaatg aaaaaattcc acctcaacta gttacagatt
     4861 tccaaaaatg tgttaaaata agtgatgaag tgaaaaaaat ccgtgaaatt gtagaaatca
     4921 tctcctaaat ggagggaaaa ccgaaatgaa attaaataaa tatatagatc atacgctttt
     4981 aaaacaagat gcaaagaaaa aacaaattga tagtttgttg tctgaggcta gagagtatga
     5041 ctttgccagt gtttgcgtta atccgacctg ggttgaacat gctaaaaaag gacttgaagg
     5101 cacagatgtt aaggtttgca cagtagtagg tttccctttg ggagcaacaa cttcagccgt
     5161 gaaagcattt gagacaaaag aagctatcca aaatggtgca gatgagattg atatggtgat
     5221 caatgttgga gctctcaaat caggtaattt agccttggtt gagtcagata ttcgcgcagt
     5281 agtggaagca agtggtgata agttagtgaa agtcattatt gaagcttgcc ttctgacaga
     5341 ccaagaaaaa gttgttgttt gccaattggc ccaaaaagct ggggctgact ttgtcaaaac
     5401 atctactggc ttttcaactg gtggtgctac gatagcagat gttacattaa tgcgtgaaac
     5461 agttggatct gatatgggtg tcaaggccgc cggtggagct cgttcttatg cagatgctct
     5521 tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca gctggggtag ctattttaaa
     5581 aggagaattg gcagatggcg actactgagt tgattgaact ggcaattgaa accagcaaac
     5641 atgcctatgt cccctattct cactttccta tcggagcagt tttagtagct aaagacggaa
     5701 gtgtttacac gggagtgaat attgaaaatg ctagctatcc tttgaccaat tgcggtgaga
     5761 gaacagctat ttttaaggct atatctgaag gccaaagaga attttcagaa ttgattgtct
     5821 atggtcaaac tgaaaaaccg atctcgccat gtggtgcttg tcgccaagtg atggctgaat
     5881 tttttgaaca agatctaaaa gtgaccttag tcgcaaaaga taaatcgacg gtcgagatga
     5941 cggtcgggga attacttcca tactctttta cagacttaaa ctagtctgag tcgctctctg
     6001 agtggcacgg tccttgtggc caaccaatcc atacttgcaa catcgttgca catcttattt
     6061 aggaggttca gtaatgaaca agaaacaatg gctaggtctt ggcctagttg cagtggcagc
     6121 agttggactt gctgcatgtg gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt
     6181 gaagacaaaa gcagcaatcg tcactgatac tggtggtgtt gatgacaaat cattcaacca
     6241 atcagcttgg gaaggtttgc aggcttgggg taaagaacac aatctttcaa aagataacgg
     6301 tttcacttac ttccaatcaa caagtgaagc tgactacgct aacaacttgc aacaagcggc
     6361 tggaagttac aacctaatct tcggtgttgg ttttgccctt aataatgcag ttaaagatgc
     6421 agcaaaagaa cacactgact tgaactatgt cttgattgat gatgtgatta aagaccaaaa
     6481 gaatgttgcg agcgtaactt tcgctgataa tgagtcaggt taccttgcag gtgtggctgc
     6541 agcaaaaaca actaagacaa aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat
     6601 ctctcgtttt gaagcaggat tcaaggctgg tgttgcgtca gtagacccat ctatcaaagt
     6661 ccaagttgac tacgctggtt catttggtga tgcggctaaa ggtaaaacaa ttgcagccgc
     6721 acaatacgca gccggtgcag atattgttta ccaagtagct ggtggtacag gtgcaggtgt
     6781 ctttgcagag gcaaaatctc tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat
     6841 cggtgttgat cgtgaccaag aagcagaagg taaatacact tctaaagatg gcaaagaatc
     6901 aaactttgtt cttgtatcta ctttgaaaca agttggtaca actgtaaaag atatttctaa
     6961 caaggcagaa agaggagaat tccctggcgg tcaagtgatc gtttactcat tgaaggataa
     7021 aggggttgac ttggcagtaa caaacctttc agaagaaggt aaaaaagctg tcgaagatgc
     7081 aaaagctaaa atccttgatg gaagcgtaaa agttcctgaa aaataatgga tggaagtcat
     7141 ttcttaggaa gcggccaagg gccgcttttt taagagttga gacaaagtca ttttgtctaa
     7201 gtaaagcttg ctactagaca aactagcaag ttttattgaa ataaaataag actctgaaag
     7261 gaagagcaca tggcacacga aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt
     7321 ggatttgttg ccaacgacaa aatcaacttg cacctacgaa aaggtgaaat tcatgcactt
     7381 ttaggagaaa atggggctgg taagtccacg ctaatgaaca tgttagcagg ccttcttgaa
     7441 ccaactagtg gtgaaatcgc ggtcaacggt caagttgtca atctcgactc cccatctaaa
     7501 gcagctagct tgggaatcgg gatggttcac cagcacttta tgttggttga agccttcaca
     7561 gtggctgaaa acatcatttt aggtagtgaa ttgactaaaa atggtgtgct agatatcgct
     7621 ggagctagca aagaaatcaa ggctctttct gaacgttatg gcttagctgt tgacccttct
     7681 gccaaggtag cagatatctc agttggagcc caacaacgtg tagaaatttt aaaaacactt
     7741 tatcgggggg ctgatatcct tatctttgac gaaccaacgg ctgttttgac tccatcagaa
     7801 attgatgagt tgatggctat tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg
     7861 attacccaca aattggatga aattcgagca gtttctgacc gtgttacagt tatccgtcgt
     7921 gggaaatcaa ttgaaaccgt tgaaattgca ggggctacca atgctgattt ggcggaaatg
     7981 atggtaggac gttctgtttc ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg
     8041 gttttgtcta tcaaagattt ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat
     8101 ctgtccttgg atgttcgtgc tggagagatt gttggtattg cggggattga tggaaatggt
     8161 cagtctgaac tgattcaagc cattacaggt cttcgtaagg ttgaatctgg tagcattgag
     8221 ctaaaaggag attcaattgt aggcttgcac ccacgtcaga ttacagaact aagtgttggg
     8281 cacgttccag aagaccgtca ccgtgatggc ttgattttgg aaatgatgat atctgaaaat
     8341 attgcccttc aaacctacta taaagaacca catagtaaaa atggaatttt gaattattca
     8401 aatattactt cttatgctaa aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa
     8461 ttagttcctg cagctgcact ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa
     8521 attgatcgag atcctgatct ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt
     8581 gccattgagt atatccacaa acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt
     8641 gttgtcagct ttgaattgga tgagatttta aacgtctcag accgtattgc cgttatccac
     8701 gatggtaaga ttcaaggtat tgtatcacca gaaacaacca ataaacaaga acttggtgtc
     8761 ttgatggctg gtggaaactt gggaaaggag aagagtgatg tctaaaaaat tacaacaaat
     8821 ttcggttccc ttgatttctg tattcctagg aattttactc ggagccattg tcatgtggat
     8881 cttcggttat gatgctattt ggggctacga agaattgttc tatacagcct ttggcagtct
     8941 gcgtgggatt ggagaaatct tccgtgctat gggtcctctg gtcttgattg gtcttggttt
     9001 tgccgttgcc agtcgagctg gtttctttaa cgtcggactt cctggtcagg ctttggcagg
     9061 ttggattctc agtggttggt ttgccctgtc gcatccagat atgccccgtc ccttgatgat
     9121 tctagcaacc atcgtgattg ccttgattgc tggtgggatt gtcggagcga ttccaggtat
     9181 tcttagggcc tatctaggga cgtcagaggt tattgtaacc atcatgatga actacattgt
     9241 cttgtatgta gggaatgcct ttatccatgc tttccctaaa gacttcatgc aaagtacaga
     9301 ttcgaccatt cgtgttgggg ctaatgcaac ctatcagaca ccttggttgg ctgagttgac
     9361 tggtaactca cggatgaata ttggtatttt ctttgccatc attgccgttg cagttatttg
     9421 gttcatgctc aagaaaacaa ctcttggttt tgaaatccgt gcagttggtc ttaatccaca
     9481 tgcttcagaa tatgctggta tttctgccaa gcggactatt atcctatcta tgattatttc
     9541 aggtgccttg gcaggtcttg gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta
     9601 tgttcaaggt tcgtcattag ctatcggatt taacggaatg gcggttagtt tgcttgcggc
     9661 caactcacca attggtatac tctttgcagc cttcctattt ggcgttctcc aagttggggc
     9721 tcctggtatg aatgcggcgc aggtaccatc tgagcttgtc agcattgtaa cagcgtctat
     9781 tatcttcttt gtcagtgttc attaccttat cgaacgcttt gtcaaaccga aaaaacaagt
     9841 taaaggaggt aagtaaagat gtctattata accttgctcc ccctcttggt gtcttctatg
     9901 ctgatttact cagcacctct catctttaca agtatcggtg gtgttttctc tgaacgtggt
     9961 ggtgtggtaa acgttggcct tgaaggaatt atggttatgg gtgccttttc tggagttgtc
    10021 tttaaccttg aatttgcaga acaatttgga gcagcaactc catggctatc cttgcttgta
    10081 gcaggattgg ttggtagtgt tttctctatc atccacgcag cagcgacggt tcatttccgt
    10141 gcagaccatg ttgtcagcgg tacggtattg aacttgatgg cgcctgcctt ggctgttttc
    10201 ttagttaaag ttctttataa caaaggacaa accgacaacc taagtcaaac ttttggacgc
    10261 tttgatttcc cagtcttggc aaatatccca gtgattggtg atatcttctt caagtcaact
    10321 agtctacttg gttatctggc gattgccttc tcatttcttg cttggtttat tctctttaag
    10381 actcaatttg gtcttcgtct ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg
    10441 ggaatcaacg tctacaagat gagatattta ggggttatta tttcaggttt tctaggtgga
    10501 attggcggag cgatttatgc gcaatcaatc tcagttaact tctcagtgac aactattgtt
    10561 ggacctggat ttatcgccct tgctgcgatg atctttggga aatggaatcc aatcggtgct
    10621 atgctttcta gtctcttctt tggactttca caaagtttgg ctgttatcgg ttctcaattg
    10681 ccgttcctac aaggagtgcc agcggtttat cttcaaattg caccttatgt tttgacaatt
    10741 cttgtcttag cagccttctt tggaaaagca gtcgcaccaa aagcagatgg tatcaactac
    10801 atcaaatcaa aataagcata caaaaaaacg tcagtccaat gactgaacta caccccaaaa
    10861 gttagacaga aaaaatctaa
//
LOCUS       DAVHEW010000072        10806 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVHEW010000072 DAVHEW010000000
VERSION     DAVHEW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.072"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..990
                     /locus_tag="VVL53_001597"
                     /pseudo
     CDS             1..990
                     /locus_tag="VVL53_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1025..1447)
                     /gene="nusB"
                     /locus_tag="VVL53_001598"
     CDS             complement(1025..1447)
                     /gene="nusB"
                     /locus_tag="VVL53_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU4183747.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(1440..1829)
                     /locus_tag="VVL53_001599"
     CDS             complement(1440..1829)
                     /locus_tag="VVL53_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4183748.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(1851..2411)
                     /gene="efp"
                     /locus_tag="VVL53_001600"
     CDS             complement(1851..2411)
                     /gene="efp"
                     /locus_tag="VVL53_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU4183749.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2540..3982)
                     /gene="gatB"
                     /locus_tag="VVL53_001601"
     CDS             complement(2540..3982)
                     /gene="gatB"
                     /locus_tag="VVL53_001601"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU4183750.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(3982..5448)
                     /gene="gatA"
                     /locus_tag="VVL53_001602"
     CDS             complement(3982..5448)
                     /gene="gatA"
                     /locus_tag="VVL53_001602"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU4183751.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(5448..5750)
                     /gene="gatC"
                     /locus_tag="VVL53_001603"
     CDS             complement(5448..5750)
                     /gene="gatC"
                     /locus_tag="VVL53_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU4183752.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            5963..7507
                     /locus_tag="VVL53_001604"
     CDS             5963..7507
                     /locus_tag="VVL53_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU4183753.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7790..7987
                     /locus_tag="VVL53_001605"
     CDS             7790..7987
                     /locus_tag="VVL53_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU4183754.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            8098..8274
                     /locus_tag="VVL53_001606"
     CDS             8098..8274
                     /locus_tag="VVL53_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU4183755.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            8390..8578
                     /locus_tag="VVL53_001607"
     CDS             8390..8578
                     /locus_tag="VVL53_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU4183756.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            8735..9100
                     /locus_tag="VVL53_001608"
     CDS             8735..9100
                     /locus_tag="VVL53_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4183757.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            9103..10770
                     /locus_tag="VVL53_001609"
     CDS             9103..10770
                     /locus_tag="VVL53_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU4183758.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         3138 a         2117 c         2441 g         3110 t
ORIGIN      
        1 caaccaaaag attgctcaaa agttgattga aaagatttct atgactgata ttgcccatca
       61 gctttccatc tcaacttcaa ctgttattcg taagctcaat gattttcact ttaaacatga
      121 tttttcttgt cttcctgaga ttatgtcttg ggatgagtat gcttttacaa agggaaagat
      181 gagcttcatt gcgcaagatt ttaacaatct caatatcatc actgttcttg agggtagaac
      241 acaagctatc attcgaaatc actttcttcg ctacgataga gttgtccgat gtcgcgtcaa
      301 aattattact atggatatgt ttagccctta ctatgacttg gctaaacagc ttcgctttca
      361 aattcctagg ctcaggctga aacagtctcc cagactgttt cactcccgaa tgctaaaatc
      421 gttcttgatc gctttcacat tgtacaacat cttagccgtg ccatgagtcg tgtgcgtgtc
      481 caaatcatga atcagtttca tcgaaaatcc catgaataca aggatatcaa gcgctactgg
      541 aaactcattc aacaggatag ccgtagactg agtgataagc gattttatcg tcctactttt
      601 cgtatgcact taaccaataa agagatttta gacaagcttt tgagccattc acaagacttg
      661 aaacaccact atcagctcta tcagctcttg ctgtttcact tcccgaataa ggaaccagag
      721 aaatttttcg gacttattga ggacaatctt aagcaggctc atcctatttt tcagactgtc
      781 tttaaaacct tcctcaaaga taaagaaaag attgtcaacg cccttaaact acactattct
      841 aatgccaaac tggaagcgac caataatctc atcaaactta tcaagcgcaa tgcctttggt
      901 tttcgaaact ttgaaaactt caaaaaacgg atttttatcg ctttgaacat caaaaaagaa
      961 aggacgaaat ttgtcctttc tcgagcttag ctttttttca acccactaca gttgacaaag
     1021 agccttattg ttcttctgtt acaaactggc tgagcagtcc attgataaaa cgggcagatt
     1081 tttgatcgga gaagtccttt gcaagctcga tagcttcatt aacagcaacc agctgaggag
     1141 tgtcaaatga agtgatttca aagactccca agcgaaggag gtttctctcc acgagcgtta
     1201 agcgttcaat ggtccaacct gcttttaaat gctgagtgat ttgcttatct agttcttcct
     1261 ttttagcttg aacaccagaa acgaggtcta tcaaaaaggc tggaagttgt acatccgtat
     1321 cttcacgatc atgagtatag gcgaaacgac aagcagtttc gacatccgta ccgaactcaa
     1381 ggctcatgag agcttgaaaa gcgcatttac ggagttggcg tctagattct aatagtggac
     1441 tagtcattga ggaagtcctc gtcaaataga tctttcaatt ctggttttgg tgttttatct
     1501 gggacgatac ctgcaacgtg aatattgata gcagcgagtt ctacatcagc catattacgg
     1561 acggcatctt tgacagcttt ctggatagca accgctacct taggaacttt tactccgtac
     1621 tcaaggtaga gatagatatc tgctgtgagt tcttcgtcca cgtttttaag ataaatgcca
     1681 cggccgagtg aaagttttga aagggtatca gacactgatc tgtttgaaaa agagtgaaca
     1741 ccctctacct ttgcagtagc gatagcaatg attttttcaa gtacacgtgg ggcgataacg
     1801 atttcgccaa gttgttcttc aattcccata gaatgacctc tttctagaga ttaggcacga
     1861 gaaacgtaag ttccttctgc agtgttgata acgagttttt gtcctgcttc gatgaagtct
     1921 ggaacgttta cgacaagtcc agtttccatc gttgctggtt taccagaacc tgtaacagta
     1981 gcacctttga tagatggttg agtttcagca actgtcaact caacagtagt aggaacggtg
     2041 acaccgatca cttcagttcc gtagaattgg attttcacat cagagttttc aaggatgtaa
     2101 agcaattcgt tttcaacatt gactacaggg atttcgtatt ggtcataagt ttctgtattc
     2161 atgaagtatg ctgtgtcatc cattttgtac aagtattgag ctgggacagt ctcgataata
     2221 gcttgttcaa atttttcctc tggacggtag cttgtgtcaa atgtagaacc agtacggaca
     2281 tcacgcaatt tcatacgcat gatcgtgttt cctttacctg gtttgtggtg actagcttcc
     2341 aaaacgcgaa tcaatttgcc gtcagctgtt tcaaaggtca taccagcttt taatttactt
     2401 gcttcaatca tgtttttacc tctttaataa atattattac tcttcatttt accataaaat
     2461 actctggaaa taaagccaaa atagttattg ggagttggta gagaagaaaa aaccaacctt
     2521 agggctggtt ttgttctgtc tagttttctt tcaacttcgc caattcctgt gcaagtagtt
     2581 taagggcaac ttgtgggttg gcttggcctt tggttgcctt cataaggaat cctgtaaagg
     2641 ccttgtcggc gttacgtttg cctgacttga agtcggcaac agcagcttcg ttatcggcaa
     2701 agacttggtg gatgattggg atcaagatag ctggatctga aatttgaacc atacctgctt
     2761 tttccacgta ttcacgcgcg ccaccgccat ttttagctag atggacaaag actttcttgg
     2821 caatcttaga tgaaatagta ccgtcttcga tgatggcaat catttcaacc aagttttctg
     2881 gtgtcaattc gatttgttcc agtgttttac cttcagcatt caagaactga gcgacttccc
     2941 cttggagcca gttagagact tgtttggcat caccacctag ggcaacagct ttttcaaaga
     3001 agtcagaagt gactttatta gcagtcaact gactagcatc gtagtctgat aaaccaaggt
     3061 cagatacata acgcgcacga cgttcttttg gaaactctgg caactcagtc cgcatttcct
     3121 caatccactc gtcagaaatt tcaaagaggg gtaggtctgg ttctgggaag tagcggtagt
     3181 cagcagcccc ttccttgaca cgcatgagga tggttgcttt attcgcttca tcgtaacggc
     3241 gtgtttcttg gcggatttga ccacctgagc gaagaatttc agcctggcgt tggacttcgt
     3301 attcaagacc tttacgaacg tttgagaagg agttgaggtt cttcaattca gtcttggtac
     3361 cgaatttctc ttgaccataa ggacgaaggg agatgttggc atccacacgc atcgaacctt
     3421 cctccatctt aacgtcagaa atgccagcgt actggataac ttccttgagg gctgtcagat
     3481 aagcataggc ttcttcagga gaacgcatat ctgcctcaga tacaatctca atcaagggaa
     3541 ccccttggcg gttgaggtca acataagagt agccatctgt accatgggtg tttttaccag
     3601 cgtcttcctc taggtgggca cgttcgatac cgattttctt ggtcgtaccg tcttctagtt
     3661 tgacttcaat ccagccatta tatccgattg gttcatcaaa ctgagaaatt tggtaggctt
     3721 tggggttatc aggatagaag tagttcttgc ggtcaaagtg catctttttg tggatgtcca
     3781 tgttgagggc aagagcagcc ttgataccgg catcaacaac ccctttattg agaactggta
     3841 gaactcctgg gaaagaccag tcaatcacgt tagtgttggc attttggtca tttccaaagt
     3901 gggcagaagt aggtgagaag atttttgaat tggtgttgag ctctacgtgg acttcaagtc
     3961 cgatgactgt ttcaaagttc attagttgtc acctccaaaa atcacgggtt gttgtttgtg
     4021 gtagtctgtt gttgcttcaa aagcagcagc agcttggtaa atggtttcct cagagtactt
     4081 gggaccaatc aattggagtc cgacaggtag accttgagag aatccagcag gaatcgaaat
     4141 tccaggcagt cctgccaagt ttacaggtat ggtcaatagg tcggctaagt acatggcaac
     4201 tgggtcatgg ttgagagaat ccaagtcata ggcaacactt ggagcagttg gacccaaaat
     4261 caaatcgtaa tccgcgaaga ctttttcgaa atcttgaatg atgagggtac ggacttgacc
     4321 agcctttttg tagtaggcat catagtaacc tgatgaaaga ctgaaagtac ccagcatgat
     4381 acgacgtttt acctcttcac caaaaccttg gcttcggctg tttacataga tttcatcaag
     4441 gttggttgca tcttctgcgc gatagccgta acggataccg tcgaagcgtt gcaagtttga
     4501 tgaagcttct gatgaagcga tgatgtaata aacggcaaca ccgtatttag agtgaggaag
     4561 gctgacttct tcgacgatag cacccaattt ttcaaagtgt ttggccgcgt ttaagattgt
     4621 ttccttaacc tctggatcaa ttccttcgcc taggtattcc ttaggcaaag cgattttcat
     4681 acccttgatg tcttggccga tttttgaagt aaagtcggcg atgcggacag gagcagaagt
     4741 agagtcttta gcatcttcgc tggcaatagc gttgagcaag agggcatttt ccttaacagt
     4801 aggagcaaaa ggtccaatct ggtctaatga gctaccaaag gcaatgagac cgaaacgtga
     4861 aactgttccg taggttggtt tgagaccaac gattccgttg aaggcagcag gttggcggat
     4921 ggaaccacca gtatcagaac caagtgacaa gcgaacttgt cctgaggcta cagctgcggc
     4981 agaaccactt gatgacccac caggaacctt gctgtggttc caagcgtttt tagttgctcc
     5041 gtagtgtgaa gtttcacctg aaccacccat agcaaattcg tccatgttgg tctttccaac
     5101 gacaatcatg cccttggttt ttgcattggc aacagctgtc gcatcaaaga ttggctcata
     5161 gttgtagagc atttttgagg cagcagttgt gagaataccg tctgtagaga tgttatcctt
     5221 aacagcaagt ggaattcctg aaaggacatt gtcagcatca attccagctt catcaatggc
     5281 tttagcttga acaagagctt gctcctcagc gatggtgaca aatgaattga gggcttcctc
     5341 acgagacttg atattttcaa gtgttgcttg ggtcaattct gttgcagaaa tttccttaga
     5401 gacaaggaga ttgtgcaact cttcaatagt tttattgtta aaagtcatta ggcatctcct
     5461 ccattgtcta ggatagctgg caccttgata tagtagttgt ctttttcagg tacgttttta
     5521 aacaagcgat cacggtctat tccttcttcg gccacatcag ggcggagtac agtcttgcgg
     5581 tcagccatag tcgtagtagg tgcgacacca gttgtgtcaa cttcgcccag caattcaacc
     5641 atgtcaacaa tcttagacaa ggtggtcgca aaggcagcag tttcttcttc agagaatctt
     5701 aattttgaaa gattggcaac gtgtgttacc tcttcttgcg taattttcat cttgcatcct
     5761 ttcgtgaaat gatgattttt agtctgttct attttaccat attttcctat aaataaggga
     5821 gggggaagtg aaaagaaata gaaattgaaa aaaatgctaa aatccgatat aatggagtgt
     5881 tgaaaggaat ggtaaaatcc aatgtaaaaa tcattctcag ctattgaaac aagaaaaata
     5941 gagaatcaaa gaaagagaac ttatgaatat tcaagaagaa attaagaaac gtcgtacctt
     6001 tgccattatc tcccacccgg acgcggggaa aacaaccatc actgagcagt tactctactt
     6061 tgggggtgag attcgtgagg ctggtacggt aaaagggaag aaaacaggga cttttgctaa
     6121 atctgactgg atggatatcg agaagcaacg tgggatttct gttacttcat ctgttatgca
     6181 atttgactac gacggcaagc gcgtgaatat cttagacacg ccagggcacg aggacttctc
     6241 agaagatacc tatcgtacct tgatggcggt ggatgctgcg gtcatggtcg tggactctgc
     6301 caaggggatc gaggctcaaa ccaagaaatt gtttgaggtt gtgaaacatc gtggcattcc
     6361 agtctttacc tttatgaaca agctggaccg tgacggtcgt gagcctttgg atctcttgca
     6421 agaattggaa gaaatcttgg gcattgctag ctaccctatg aactggccta tcgggatggg
     6481 gaaagccttt gagggcttgt atgacctcta taaccaacgt ttagagcttt acaaagggga
     6541 tgagcgtttt gccagtctag aagacgggga caaactcttt ggtagcaatc ctttctacga
     6601 gcaagtcaag gatgacattg agcttttaaa tgaagccggg aatgagtttt cagaggaagc
     6661 tattctggct ggagaattga cgcctgtctt tttcggttca gccctgacaa actttggtgt
     6721 gcagaccttc cttgaaacct tcctcaagtt tgctccagaa ccacatggtc acaagaaaac
     6781 agacggtgaa attgtggatc cttatgacaa ggatttctca ggctttgtct ttaaaatcca
     6841 agccaacatg gaccctcgtc accgtgaccg tattgccttt gtccgtatcg tatctggcga
     6901 atttgagcgc ggcatgagtg tcaatctccc tcgtactggt aagggggcta aactatctaa
     6961 tgttacccag tttatggcgg agagtcgtga gaatgtgacc aatgccgtgg caggtgatat
     7021 tatcggggtt tacgataccg gtacttatca ggttggggat accttgacgg ttggaaaaaa
     7081 caagtttgaa tttgaacccc tgccaacctt tacacctgag attttcatga aagtttctgc
     7141 taagaatgtt atgaagcaaa aatccttcca caaggggatt gagcaattgg tgcaagaagg
     7201 agccgttcag ctttataaga attaccaaac aggtgagtac atgctaggcg ctgtaggtca
     7261 actccagttt gaagtcttta aacaccgcat ggaaggcgag tacaatgcgg aagtagttat
     7321 gaacccaatg ggtaaaaaga ccgttcgttg gatcaagcct gaggacttgg atgaacggat
     7381 gtcgtcaagt cgcaatatct tagccaaaga ccgttttgac caaccagtct ttctctttga
     7441 aaatgacttt gccctccgct ggtttgcgga caagtatcca gacgtagagt tagaggagaa
     7501 gatgtgactc agtaccaaca attggaacta aagttccaaa ttgttggacg ctagtcgcta
     7561 tttggcgaac tagctaactg cctcactaac tacgtttggc aaatgaaatc gatttgtaaa
     7621 actccgtgtc gtaatgtaaa aattgtaaac cgtaatggga cttcatctaa aatctccact
     7681 ggtatggttt tacgaacgaa taggcgaaaa taatggttac gtagaaacat atgatgggaa
     7741 acatatgatg ggaataatat gatgggaata aaaagaaatg agataaaaag tgaaagaaga
     7801 gaaaaaagcg attgtcttag gtgcagataa tgcttatatg gataaagttg aaacaacaat
     7861 aaaatctctt tgtgttcatc attataatct taagttttat gtttttaatg atgaccttcc
     7921 gcgagaatgg ttccaattga tggaaaaaag actagagact ttgaatagtg agattgtgaa
     7981 tgtttagatt gacagtaata ttctgaaagg ttatcgtctc ccctttgagg gactttcata
     8041 tgcggctttt ttaggtattt tattccaaag tatgtaagcg agtctcgtgt actgtatttg
     8101 gattcagata ttgttgtaag aaaatcgata gatgagctgt gggatttgga cttgacggct
     8161 atacccttag ctgcagttag agatgatttt tatacacata attttaattc tggtgttctg
     8221 ttaatcaacg atggcatgtg gagagcagaa aatgtcacgc aagacctgat ttaagacaca
     8281 aataaaaaat cttccaattc ccttgcaaaa aacaagaaaa tgtgataaca tagtacggta
     8341 tgtggtgcta gcacatccgc tatattagat ctaataggag gaaaacacaa tggctaaagt
     8401 atgttacttt acaggtcgta agactgtatc aggaaacaac cgttcacacg cgatgaacca
     8461 aacaaaacgt gccgtaaaac caaaccttca aaaagttact gttcttatcg atggtaaacc
     8521 taaaaaagtt tgggcttcag ctcgtgcttt gaaatcaggt aaagttgaac gcgtttaata
     8581 ataaaagcaa aggagacctt agggtctttt ttcttttatt gtgggtataa catcgtttga
     8641 aaaatagagt aaatatccgc tgatacatca ttctgctttt acacttgggc tgaaatatga
     8701 taaaatagag tatcaactag ttgaggtaaa aaggatgact gtaaaaatta atacaaaaga
     8761 tggtcaaatc gaactaacag atgaagtgat tgcaaccgtc gtaggtggag cagcaactga
     8821 gatttttggt gtggtcggta tggctagtaa aaatgccctc aaagataatt tccaagccct
     8881 gctaggtaag gaaaattatt ctaaaggtgt cgtcgtaaag gcagccgaag atggcagtat
     8941 tgcagttgat gtatataccg tgttaagcta cggaacaaag attagtgaag tgtcaaaaaa
     9001 cattcaagag cgtgttgcct ttagcttgga aaaccagctt ggaattactg ctcagactgt
     9061 aaatgtctac attcaaaata tcaaagttgt aggagaataa ccgtgtcaaa aattactact
     9121 agcttatttc aagaaatggt gcaggctgca tcaactcgct tgaataagca agctgaatat
     9181 gtcaattcat taaacgtctt tccagttcca gatggagata ctgggacaaa tatgggaatg
     9241 accattgaaa atggtgctaa agaagttgca gacaagccag cttctacagt tggagaagta
     9301 gcgagcattc ttgccaaagg gcttttgatg ggtgcgcgtg ggaactcagg agtgattacg
     9361 tctcagcttt tccgtggatt ttcccaagct atcaaggata aagacgagtt aacaggtcaa
     9421 gacttggctc tggccttcca atcaggtgtg gaagttgcct ataaggcagt gatgaaacct
     9481 gttgaaggaa cgattttaac agtttctcgt ggagctgcta tcggtgctaa gaaaaaagct
     9541 gagcaaacag atgacgctgt tgaagtcatg cgcgcagcct tggaaggtgc taaaacagct
     9601 ctagctaaaa caccagacat gcttccagta ttgaaagaag ttggcgttgt ggactcaggt
     9661 ggtcaaggat tggtcttcat ctacgaaggt ttcctttcag cccttactgg cgaatatatt
     9721 gcatctgagg actttgtagc gactcctgcc aacatgagtg agatgatcaa tgtagagcat
     9781 cataagtctg tagctggtca cgtagcgact gaggacatca cgtttggtta ctgtactgaa
     9841 atcatggtag ctcttaagca aggtccaacc tatgctaaag attttgacta cgatgaattc
     9901 cgtaactact tggatgagct tggagattct ctccttgttg ttaacgatga tgaaattgtc
     9961 aaagtccatg ttcatacaga agatccagga cttgttatgc aagaaggtct caaatatggt
    10021 agcttggtca aggtaaaagt tgacaatatg cgtaaccaac acgaagcaca ggttgagaaa
    10081 gaagctactc aagttatcaa gtcggctgaa gaaaaagagt atgctttgat tgctgtggtg
    10141 gctggtaaag gtctagcaga tatcttctgt tctcaaggcg tggattatgt tatcgaaggc
    10201 ggtcaaacca tgaacccttc aacagaagac tttatcaagg ctgttgaaca ggttaatgcc
    10261 cgtaacatca tcttcttgcc aaacaacaag aacatcttca tggcagctca atctgcggca
    10321 gaagttttgg agcaaccagc ggtagtggta gaggctcgca ctcttcctca aggtatgaca
    10381 agtcttcttg cctttgatcc aagcaagtcc attgaagaaa accaagagcg tatgacagct
    10441 gctcttagcg atgtcgttag cggaagcgtc acaacagccg tgcgtgatac aacgatcgat
    10501 ggcttagaaa tccatgaaaa cgataattta ggtatggtgg atggaaaaat tcttgtgtca
    10561 aaccctgata tgcaccaaac attgactgaa accttgaaac atatgttgga tgaagacagt
    10621 gaaatcgtaa ccttctatgt aggtgaagac ggaagcgaag aacttgccaa tgaaatcgct
    10681 caagaaatcg tagaagaatt cgaagacgtt gaagtcgaga ttcaccaagg tcaacaacct
    10741 gtttacccat acctatttag tgtggaataa gattaatgaa ggactgagaa atcagttctt
    10801 ttttct
//
LOCUS       DAVHEW010000073        10685 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVHEW010000073 DAVHEW010000000
VERSION     DAVHEW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.073"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(95..1519)
                     /locus_tag="VVL53_001610"
     CDS             complement(95..1519)
                     /locus_tag="VVL53_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU4183759.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2064..4343)
                     /gene="pulA"
                     /locus_tag="VVL53_001611"
     CDS             complement(2064..4343)
                     /gene="pulA"
                     /locus_tag="VVL53_001611"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU4183760.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4455..6413)
                     /gene="ligA"
                     /locus_tag="VVL53_001612"
     CDS             complement(4455..6413)
                     /gene="ligA"
                     /locus_tag="VVL53_001612"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU4183761.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFAAEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6506..7684)
                     /locus_tag="VVL53_001613"
     CDS             complement(6506..7684)
                     /locus_tag="VVL53_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4183762.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7764..8627)
                     /locus_tag="VVL53_001614"
     CDS             complement(7764..8627)
                     /locus_tag="VVL53_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU4183763.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8630..10531)
                     /locus_tag="VVL53_001615"
     CDS             complement(8630..10531)
                     /locus_tag="VVL53_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4183764.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKSANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3206 a         2359 c         1906 g         3214 t
ORIGIN      
        1 attataatac caaaaagttc gtattttttc tataattatt attattttat agcaaaaaaa
       61 taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa acaatggatt
      121 tgacatttgt catcgcttca atgctatatt taattccttg cactccagct ccagaacctt
      181 tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta atgtggactg
      241 tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt gtaaagactg
      301 atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct acactagcca
      361 cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc atatcttttg
      421 taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt ggtgtaagag
      481 cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg gctgaagcat
      541 tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt aatttagaaa
      601 cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca atgacacgtt
      661 taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt tgcttggcag
      721 catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca agttccaaca
      781 tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt gaacctgtaa
      841 agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct gaaccacgac
      901 ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct ttagccaaca
      961 agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc cctgcaatca
     1021 aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga gcaatcgcta
     1081 gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt gttgcctcaa
     1141 aaccaccacc ttccattgct tgtccagtga tacggagacc ttcctcagca gcataacgaa
     1201 tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt gctacttctt
     1261 tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct gttttatgca
     1321 aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct gcacgcgcag
     1381 cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc aattcttctt
     1441 gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt ccatttacta
     1501 aattctgata tcttgtcaaa tttttgtcct cctgaaaatg attaagatat attttaccaa
     1561 attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca caaacttgat
     1621 ttttcaaagt atactcaata aaaattaaaa aaatccacta cgtcaaggcg aggctaatgt
     1681 ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa taaaaaaact
     1741 gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa acaagggtgt
     1801 gctattccaa tttcagccta ctataactgt catagattgc tgaaacaaag tctaggtaaa
     1861 agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag gaggtcttgt
     1921 tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg ttctttctat
     1981 tctttcaatt tcaatgaatg gattttttac taatactcat aactgggaat ttgtctgtgt
     2041 aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact aatatcattt
     2101 aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt tcctgcattt
     2161 tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc tttgccgtga
     2221 acttcataga taaggtagcc gctatgttca atcgcagaat gcacaaagac atgatggtaa
     2281 atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg gatgacttga
     2341 cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac ttggttcaca
     2401 ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt caactcacca
     2461 ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc cataaaggcc
     2521 atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat gatttgctct
     2581 gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt gtcatgggct
     2641 tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg acttcctagg
     2701 attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga cttgatagca
     2761 ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa accaatattt
     2821 ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc tgttcccata
     2881 tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct ttgacgaatc
     2941 atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc gtcaatatta
     3001 tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat ttcgtgttca
     3061 ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg attcatacga
     3121 taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa ggtatggtta
     3181 tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac catcaccttc
     3241 aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt ttctggcgcg
     3301 ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc atcgtattct
     3361 ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt cttgatgtaa
     3421 tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc acccaagaaa
     3481 gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc acgaatatgc
     3541 atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc cgaaccgtgc
     3601 ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga acgtttgcca
     3661 tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg agggaattgg
     3721 acttgatact gataagtctt acctaccaaa tcttcttcaa catccaaact ccagacaccg
     3781 attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc aaaagtcttc
     3841 caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc tgtcgctgta
     3901 ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa ttctccttgg
     3961 taaccccaat gatgatcaaa actagcactg ttaatggcct tatcaaaggc aaaaggattt
     4021 tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat cctatcatcg
     4081 ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat agaatattct
     4141 ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg tgaacttggg
     4201 tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa atcaattcgt
     4261 tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg atggtaatga
     4321 atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt ctatttcact
     4381 aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc taaactctgt
     4441 ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg gataccaagt
     4501 tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag gtcggtcttt
     4561 ttggaaacac tacctgtcac tttggcaccc agactttcga gtttactttt agcttctgag
     4621 cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa ggccgcatcc
     4681 gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa ttctctgagc
     4741 agaatttcag agccttctgc cgcaaaataa gtctgaagac ttttggcaat cacgccacct
     4801 agactttcaa tactagccac ttcctctgaa tctgcctgat acagattttc aattgaatgg
     4861 aaatattgaa gtaaaagctg actagccttg cttccgacat gacgaattcc caaaccaaat
     4921 aagagcttct cggcagaatt ttcctttgat gcttggatag cctgatacag tttagcagcg
     4981 gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg ataaatatcc
     5041 gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg accaaggcct
     5101 gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat ttgagcaggg
     5161 caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa gtcagagtta
     5221 caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt ggactctacc
     5281 acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt atcgtctttt
     5341 cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct aacagtcgta
     5401 ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt acggccaact
     5461 gtccagtcaa ctgataagag ttgagcttct ttttcttcag cagggaactt gtaggctact
     5521 gcccactttg gagccttaac ggtaaaacca agttcttctt gacttgctag gtcgttgacc
     5581 ttgattacca ctccatcaat atcgtaaggc agattttccc gttcctgtcc tacttcttgg
     5641 ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt gaccacaaaa
     5701 cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga agggctggct
     5761 tcttgataga gaaacgttgc aagattacgc ttggcaacta ctgctgtatc caactgacgc
     5821 agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt ttcttggcgc
     5881 acttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg aactgtgata
     5941 tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt ttctgtgata
     6001 ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc cttttcataa
     6061 gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg atgagccact
     6121 tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc atcctgcaaa
     6181 ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt gccaccaaca
     6241 cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa ctcgaccaac
     6301 tcacggtaaa ggcggtcata ctcactgtct gaaaccgagg gattatcgct ggtatagtac
     6361 tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt cataagacca
     6421 ttttaccata aaacaagctc tcctcacaaa cgagaagggc ggaaaaaaca cttagtttga
     6481 aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt cgaacataaa
     6541 taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa gaagagagat
     6601 gtacacttgg caagactgtc ataaatcctt ttgcaatagg cataaataga atagctaagg
     6661 taaaaattgt actcagtact cttccaagaa attcgctctc aaccttggtt tgtacttgag
     6721 taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt ccagaaaagg
     6781 aaagaaaagt tggaagtggt aatcccatca taaaaactcc gacacctgtc aaagccagta
     6841 aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc ccaatgatgg
     6901 aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg taaagctgat
     6961 tcgaaaaggg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg gaagctacca
     7021 gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc ttgatatcta
     7081 caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc tcttttggaa
     7141 ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt agcgtcatat
     7201 ggagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca cctacaacct
     7261 gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc accacttcag
     7321 ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagcctgc acaatgttag
     7381 caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata gccagacaaa
     7441 gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa cggtctgaaa
     7501 taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc tgatacattc
     7561 ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt ccataatcat
     7621 agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact gcatagcgat
     7681 tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa ggagcttttt
     7741 attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg ttcctgataa
     7801 taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctc ttgcatctgc
     7861 ttacagcctt ctttacactg tcctttttga tataaggcaa aaccttttaa ataatggaaa
     7921 acattacgct cataaagctt aatacctttg tcaataatct tctctgtata agcctcaaaa
     7981 tagttggcat tataaaaaga agaatgctct aaacaatgct ggtaacaatt gagggccaaa
     8041 atcaacacta atctcttatg gcgactaatc tcttggtaaa attcctccct ctccataact
     8101 tctctaccaa tccgagtgac atagtctaca tcgtagaaac tatagaggtt accgaaaaga
     8161 atcaactcat acatggtcca ttcttctgtt ttgaagagat aatctgctac cttatccaaa
     8221 tcatcctgct tcatatcata actcgcatct ctttgacaaa tcagaccttg tagcaaaatc
     8281 cagttcagct caaaataaag gggagtcgtc gaactcttag acttttcaag ttgttctctt
     8341 tgaagctttt gaaaacctgc aatatcgttt gaatagtaaa gtgggataat ctgtgccatc
     8401 atagacacat gttcatgatt atgaaaattc cttgccttat ccatgaaatt ttcgattgtt
     8461 acatgaatgt tatccaaaat ctcaaagaaa cgggagactg ccaggtcaga ctccccaagc
     8521 tcaaagcgag ataactgaga ggtagagcag gattcgcctg ctgcttcctt taaagaataa
     8581 tttccacttg ttcgaaattc acgaaatact tttccaagat gttccatctt tacacctgct
     8641 ctgataattc ttcccactca agcatagctt cttcctgacg atggctgatt ttgtccagct
     8701 cagcctgtaa ttccatgagt ttgtcggcat cgtttgtttc caacatttgt tcagaaatgg
     8761 cttggctttg actttctagc tcttcaattt cagcttctag actttcgatt tgtcgcatga
     8821 gtttgcgaac ttctttttga ctttctttct gggcctgata gtcattgact ggacttgctt
     8881 cctttgcttg attgctagtt gaagcttcct cagtctgact catttctgct gttgctttct
     8941 tctcaacata gtagtcgtaa tctccaaggt agagagttga accattctca gacaattcca
     9001 aaacatgagt tgccacacga ttgataaagt aacgatcatg actgacaaac agcaaggttc
     9061 catcaaagtc aatcaaggca ttttctagca cttccttact atcaatatcc aagtggttgg
     9121 tcggctcatc cagaatcaaa aagttattgt tttccataga caatttagct aaaagcaaac
     9181 gagctttttc gccaccagat agcatgccga ctgatttttt aacatcatct cctgagaaaa
     9241 ggaaggctcc aagacggttg cggatttcaa cttctggtgt cagtttgaaa tcattccaga
     9301 gttcatccag caccgtatta cttggtgtca gcttgctttg ggtttggtca tagtaaccaa
     9361 cctcaacatt agcgccaaag cgcttttctc ccttgataaa aggaatctgg tccacaatag
     9421 acttgataaa ggttgacttg ccgataccat ttggaccaac gatagcgaca gcattcatct
     9481 tacgaagatc taggttaatc ggttgtgaca agacttcccc gtcatagcca acagctgcat
     9541 tttcaacagt caaaacaaca ttgcccgacg ttttttcaga ctggaaggtc atgttggctg
     9601 atttcttgcc agcttcaggc ttgtccaaac gttccatttt ttccagttgt ttacggcgag
     9661 attgagcacg tttagtcgtt gaagcacgaa ctagattgcg attgacaaag tcttccagag
     9721 cagcgatttc cttctgttgc ttttcatagt tttttgcctc agtaactagc ttttgctcct
     9781 tcaattcgac aaaacgagag taattcccca catagcgatc caaggaatgc ttggtcaaat
     9841 ctagcgtaat tgtcgcaacc ttgtccaaga aataacggtc gtggctgacg ataatgaggg
     9901 caccgctata gtttaccaag taattctcta gccaggcgat ggtttcaata tccaagtggt
     9961 tagttggctc gtccaagacc aagagattgg gcttttcaag gagcattttg gcaagtgcca
    10021 aacgagtatt ttgaccacca gaaagctcag caattttcat ctgccacata gactcgtcaa
    10081 acttgaatcc attcaaaatc gctcgaatat cagcttcata ggtaaagcca cctgcttggc
    10141 gaaaattctc agataagcgg tcataatctg acatcagttt atccaaatcc tcaccagact
    10201 tttcacccat ctccagctcc atctgacgca gttgtctctc cgtccgacgc aaatcattaa
    10261 agacatgaag catttcatcg tagatggtat tttcagactc aaaacggcta tcttgggcta
    10321 ggtaagacag agaaatatct tttttcttat tgatttctcc gctagttggc tcctcttctc
    10381 caactaaaat cttcaaaaga gtagacttac ctgcaccatt tttcccaaca agagcaatcc
    10441 gatctcgttc atcaacctgc aggttgatat tatcgaaaag aacctctcct gcaaaagaac
    10501 gttcaatttt attagcttgt aaaataatca tacaagtagt atagcatgtt tccctaaggc
    10561 attcaagata atcgtaagtc ttttagtaca acttttataa cataaaataa actaaattat
    10621 gtatatttta tattagatta cttcactatc ttgttggatt ttctaaccag ctaatcttgt
    10681 ttcaa
//
LOCUS       DAVHEW010000074        10538 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVHEW010000074 DAVHEW010000000
VERSION     DAVHEW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.074"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(46..1368)
                     /locus_tag="VVL53_001616"
     CDS             complement(46..1368)
                     /locus_tag="VVL53_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU4183765.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILITGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1459..1547)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1710..4046)
                     /locus_tag="VVL53_001617"
     CDS             complement(1710..4046)
                     /locus_tag="VVL53_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU4183766.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4150..4698)
                     /locus_tag="VVL53_001618"
     CDS             complement(4150..4698)
                     /locus_tag="VVL53_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU4183767.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4695..4997)
                     /gene="zapA"
                     /locus_tag="VVL53_001619"
     CDS             complement(4695..4997)
                     /gene="zapA"
                     /locus_tag="VVL53_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU4183768.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSHEIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5084..5965
                     /locus_tag="VVL53_001620"
     CDS             5084..5965
                     /locus_tag="VVL53_001620"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU4183769.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5969..6583
                     /gene="lepB"
                     /locus_tag="VVL53_001621"
     CDS             5969..6583
                     /gene="lepB"
                     /locus_tag="VVL53_001621"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU4183770.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6817..9183
                     /locus_tag="VVL53_001622"
     CDS             6817..9183
                     /locus_tag="VVL53_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU4183771.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(9231..10514)
                     /locus_tag="VVL53_001623"
     CDS             complement(9231..10514)
                     /locus_tag="VVL53_001623"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU4183772.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         2990 a         2407 c         2167 g         2974 t
ORIGIN      
        1 aaaattattt ataggcaaaa aaatcacacg agctgtgtga ttccattatt tgtcaaaata
       61 ctttttagtt tcagcaataa cgactggcga caagaccaag agggcaatca agtttggcag
      121 agccatcaag gcgttaacga tatctgcgat aatccagacc atatccaact cgataaatcc
      181 tcctaacaag accatgagca caaaaaccac acggtagagc cagataaagc gagccccaaa
      241 gaggaactca aaacagcgtt ctccgtaata gttccaacct agaatcgttg taaaagcaaa
      301 aagtacaagg aagatggtca agagggcagg cccaaagtgt gaaaagactg ttgagaaagc
      361 tgactgagtc aaggcaaccc cattcaagtc accactccaa actccagtta tcaagatggt
      421 caaaccagtt agagtacaaa tgatgagggt atcaataaag gttcctgtca tggaaatcaa
      481 accttgctct actggttcat ttgtcttggc agctgcagct gcaataggag cagaacccag
      541 accagattcg tttgagaaca caccacgcgc cacaccattt tgaatagcca tccgaacgct
      601 agcaccagca aatccaccta ccgcagcaag gggactaaaa gctgaggtaa agactaaagc
      661 gattgtgcca gggatttttc cgatattaaa gaaaataact gtaagagttc ctaagatata
      721 aatgagggcc ataaaaggaa caacagtagt tgaaacctta gaaatagact tgagtccacc
      781 aaagactgca atcgctacaa agacagacaa gacgagagct gtgatggctg gcgaaatcgt
      841 cgttgtattt tggatagatt ctgtaatcga gttgacttgg gtgaaggttc cgattcccaa
      901 gagagcaacc aatactcctg ctactgcaaa caaaacagca agtggtcgcc acttttctcc
      961 catccctaga aggatatagt gcatgggacc tcccgctact gcaccatggt cgtccttggt
     1021 gcggtatttg atggccaaga gtccttccgc atacttggta gccattccaa agaaagccgc
     1081 catccacatc caaaatagag ctcctggtcc accaaccttg atagccgtcg caactcctat
     1141 gatatttcct gttccaacag ttgatgccaa ggctgtacac agagctgcaa aactggatac
     1201 atcaccatgt cccttatcct ggataaaaat aagctgaaag gccttgggca gacgcaaaac
     1261 ctgcaagagt cctagccgca tggttaggta aatccctgtt ccgaccaata aaatcaagag
     1321 gggcggtccc caagcaaaag catcgattga tttaagcaat tctaacattt ccttctccta
     1381 tcgtttcaac cccaaaagaa agagcacatg caagatacat gtactctgga atgcttagat
     1441 aaatgctaaa aagcggtcta tcctagctct gtccttttac ctgagagttt gagcagttgc
     1501 ctgccttgcc ccttcggtgc ctttacggtc tctccagagt tccgtccatt tacagtcatg
     1561 gaaaatcaaa cgattcccca cttctattaa acttcattcg gtgttggtat taattgattc
     1621 taattttaca aaaaatgttg gcttttgtca atgtgtttat tagtaaaaat tagttcaaca
     1681 gtttttactt tataaagtcc agaatactgc tatcctttaa aagtgacaat agtcgcacca
     1741 ctgcctccag cattttgtgg ggcatagccg aaactcttga catgtttgtt tctttgcaag
     1801 tatttggtaa ctccttcacg gatgactcct gttccgatac catggatgat atcaacttga
     1861 gccatattgt taagcaaggc ttggtcgatg aaggtatcta gctcattcat ggcttcttca
     1921 tagcgcttgc ctcgaagatc cagtctagct tgaggtcctc gcccagaagt tcgtttcaca
     1981 acattgacct gtttcttctt gactggtttt tcttgctggg cttgaacaag atcaaactct
     2041 ttctcttcca aggtcatctt aatcaagcca acttgggctt cccagcgacc gtccttgagt
     2101 tgactggtca aggtaccacg ctgaccataa ctgagaacca cgatatcatc tcccaccttt
     2161 ggagctcgtt ttttcttggc cttttgaagg accttatttt tagacaagtc cactttttca
     2221 ggagccaatt ttttcaactt ggccttggct tcaatgattt cgtggggctt gagttgggat
     2281 ttactgtgga gatttttgag aatctggtca ctttcactta gggccatatc cacaatctca
     2341 gcagcctgtt cacgcgcctt gttaagctcg gtttcctttt cacgattaag ctcgttgtag
     2401 agttttttta gcgcacggtt catcttgaga ttttcttgct ccacctcacg gatattgtcc
     2461 aaacgtttgc ggctttccag cgtctgctct tctaattgct caatgatacg attgacgtca
     2521 ttgtcctgat cgatctgctg actggcatct cctacgataa cttcagatag gcctagacgt
     2581 ttggcaattt caaaggcatt acttcggcca ggaacaccct gcataaagcg ataggtcggg
     2641 cgaagagttg cagtatcaaa ctccatactg gcattttgca caaaggctgt ctcaataccg
     2701 taggccttga gttctggata gtgggtcgtc gccatggtct tgatttgacg caggcgaagg
     2761 tcctccagaa tagccatggc aagggctgct ccctcttggg gatcagtacc agcccccaac
     2821 tcatccaaaa gtaagagtga atgttggttg accttgccaa gaatatccac gatattggtc
     2881 atatggctag agaaggtaga caagctctgc tcaatagact gctcatctcc aatatcagca
     2941 aagatttctt caaaaatacc aacacgactt cccttgtctg ctaaaatcgg caatcctgac
     3001 tgggccatga cctgtgtcaa gcccagagtt ttgagcatga tggtcttccc acctgtattg
     3061 ggacctgtaa tgacaatagc tgttaaatct tgaccaaaat agacatcatt tgcgacggca
     3121 tttttgacca aaggatggca gacatggagc agttgaatct cttgattttc tgacagctga
     3181 ggcacgactg cttgtctttc ttggataaat cgaaccttgg cacgaatcaa gtccagatga
     3241 ccgataatcc aagcgtcatt agcaatctca gccgcatgag ggcggacacg ctcagaaatt
     3301 tcttggagaa tgcgaagcat ttcatagcgc tcatctgctc gcagactagc aatttcttcg
     3361 ctcagtttga ctacctcacg tggttcgata tagacggtgt ttccactagc agaaatatca
     3421 tgaacgacac ctgcaatctt attgcggtag gtgtttttga ctggtaaaac ctgacggcca
     3481 tttctgctag caacaattcc ttctgtcaac atctgcgctt tttgcttgag caagtcttgt
     3541 aaaacatcgc gtacctgact ctcgctatca tgtatttttc gacggattcg cgccaattct
     3601 tcactggcaa aattttcaat gaaacccgca tcattaaagg cctgaagatt tccttgtaat
     3661 tgcggaaaat catgtaattt ctcaaaccaa agggctaatt cttccaagct gacattttcc
     3721 agattggcgt aaaaactttg aagttctcgg ctggcaagaa gcacgcgttt caagagtagg
     3781 aactcctcga tattgagatc cgctcccatc tccaacctct tgcagactcc tgcaatttcc
     3841 ttagttgaga gaatagtaaa atgcggttgc tcgacgaaaa gagcctgcat ttccttcatc
     3901 tcagcaaaag cctgtttgat tttatctgct ttggcagtcg gagccagttg tctcaattgc
     3961 tccaagccct gctcggtcaa caaatgaggc tcaaacaagg ccttgacctt atcgaactct
     4021 aatgtttcta atattttctt attcatcttt atttccttgt ctttgaatgt ctaggtgtgg
     4081 tagcttttta cattctgata gattcgagtc aaatctgtaa acaaagggct aggaaaactc
     4141 ctgccctttt tatccgatta aatttgtcac ccagatttgt ttgagccaac tggttgttat
     4201 cggtatgctc tggatgatgt gttttgcgac gatactcttt tcaagaggat tttgtataac
     4261 tgccatgggg atggtcgcca agattgtcaa ggccatttgc aagacaaata aggtcaccaa
     4321 catggacaag atacctgctg aaacttggaa caacttacca cccagttttt tgctaggaat
     4381 caagtgtaag agaagaccaa gcaaacgacc aatgctatag acaatcccaa atacaagcaa
     4441 gtagccgatg cctgcataaa agaccttatc cagctgaaag agttgatccg atgggaaaaa
     4501 gaaagtcccc tgaccttcct gcgaatttgc ataagggagc aataaatgga attgctctcc
     4561 aagcccctta taaaactggc cagccataaa agccgatgcc atggctgaaa tcaggtaata
     4621 aacctgtaag agcaggcctc tccgatagcc gatataaaat ccccaaacca agaccaatag
     4681 aagaaggaat gaaatcataa ggaatcctca atcttgctct gttcttgctt acaagtcaca
     4741 agcttgtgac ggagttcttc tagctcttgc tccttatcgt caaattcaat ctcatggctg
     4801 agctgagttg ataaacagtt gactgccaac aaaagagcga ttgtttcatc atctgcgcta
     4861 ggcatttgtt ctttaattgc ttggtatttt tctgtcgcaa ccttagcgat ttcctccata
     4921 aaaaggttat catgttcgct tgtcaaggtt aacgattttt tcccgaatgt aaatttgaat
     4981 cgatttagat ttgccataaa attcacctca cgatattata ccaaaattcg ctaattttgt
     5041 cagtttttac aaattttact gcttttgtgg tacaatagaa actatggcaa gtatcacact
     5101 cacaccaagc gaaaaggata ttcaggcttt tcttgaacac tatcaaacca gtctggctcc
     5161 tagcaagaat ccctatatcc gctacttttt gaaactacct caagcaacgg tttctatcta
     5221 tacttctgga aaaatcttgc ttcagggtga aggggctgaa aaatacgcca gtttctttgg
     5281 ctatcaagct gtagagcaaa ccagcggaca aaatcttcct ttaattggga cagatgaggt
     5341 gggaaatggt tcctactttg gtgggcttgc agttgtggct gcctttgtca cacctgacca
     5401 gcacgacttt ttacgaaaac tcggtgtggg ggattctaag actctgaccg accaaaagat
     5461 ccgtcagatt gctcctattc tcaaggaaaa aattcagcac caggcactcc ttctctcacc
     5521 cagcaagtac aacgaggtca tcggagaccg ctacaatgct gtttcggtta aggttgccct
     5581 ccataatcag gctatctatc tcctccttca aaaaggtgtt cagcctgaga aaattgtgat
     5641 tgatgccttt accagtgcta aaaattatga caagtacttg gcacaagagg ccaatcgttt
     5701 cagcaatcct atcagcttag aagaaaaggc tgagggcaaa tacttggctg tcgcagtttc
     5761 ttctgtcatt gcgcgtgatc tctttctgga aaatcttgaa aatctgggac gagaactggg
     5821 ttatcagctc ccaagtggag ctggaacagc ttctgacaag gtggctagcc agattttgca
     5881 agcctatggt atgcagggac tcagcttctg cgccaaattg cactttaaaa acactgaaaa
     5941 agcgaaaaaa cgcttagaaa ggtaagttat gaattcattt aaaaatttct taaaagagtg
     6001 gggattgttc ctcctaattc tgtcattact agctttaagt cgtatctttt tttggagcaa
     6061 tgttcgcgta gaaggacatt ccatggatcc gaccctagcg gatggcgaaa ttctcttcgt
     6121 tgtaaaacac cttcctattg accgttttga tatcgtggtg gcccatgagg aagatggcaa
     6181 taaggacatc gtcaagcgcg tgattggaat gcctggcgac accattcgtt acgaaaatga
     6241 taaactctac atcaatgaca aagaaacgga cgagccttat ctagcagact atatcaaacg
     6301 cttcaaggat gacaaactcc aaagcactta ctcaggcaag ggctttgaag gaaataaagg
     6361 aactttcttt agaagtatcg ctcaaaaagc ccaagccttc acagttgatg tcaactacaa
     6421 caccaacttt agctttactg ttccagaagg agaatacctt ctcctcggag atgaccgctt
     6481 ggtttcgagc gacagccgcc acgtaggtac cttcaaagca aaagatatca caggggaagc
     6541 taaattccgc ttctggccaa tcacccgtat cggaacattt taagaaacct aagaggccga
     6601 gaatcaccaa tctcagcctc ttcttctatc gtgagaagtg attgtttatt gacaaatact
     6661 caatgaaaat caaagaacaa actaggaagc tagccgtagg ctgcttaaaa cagtgttttg
     6721 aggttgcaga tggaagctga cgtggtttga agatattttc gaagagtata aaataaaaac
     6781 aatcccaact ctcacctatt catgcaaagg aattttatgg aagtttattt ttcaggaacc
     6841 attgaacgga ttatttttga aaatcccagc aatttttatc gcatcctcct cctagaaatc
     6901 gacgatacgg acgcagagga ttttgatgac tttgaaatca ttgtcacagg aaccatggct
     6961 gatgtaattg agggcgaaga ctatactttt tgggggcaaa ttgtccagca ctccaagtat
     7021 ggagaacaac tgcaaatcag tcgttatgat cgcgcaaaac caactagtaa gggcttggtc
     7081 aagtactttt caagtagcca tttcaagggg attggtctca agacagctca gaaaatcgtg
     7141 gatacctatg gcgaaaatac cattgacgaa attttgcaac acccagaaaa gttagaaggc
     7201 atcgcaggac tctctgccaa aaatcgcgag gctttcgtct ccactctccg tctcaactac
     7261 ggtacggaga tggttttggc caaactagcc aactacggca ttcccaacaa actagccttt
     7321 cagattcaag acttttacaa ggaagaaacc cttgatgtgg ttgaaaatta tccctaccaa
     7381 ctggttgagg atatcaaggg tttgggcttt accattgctg accaactagc tgaggaacta
     7441 ggcatcgaaa gtcaggctcc tgaacgcttc cgcgccggtc tagttcacag tctttttcag
     7501 gcctgtatgg aaacaggaga cacctatgtt gaagcacggg atttgctgga acaaaccctt
     7561 actctccttg agtcttcccg tcccgtggaa ctagacccca gccaagttgc ccaagagctc
     7621 tcctacctga tcgaagaaga caaggttcag cagattgata ccaagatctt tgacaacagc
     7681 ctctttttcg ctgaggaagg catccgcagt cacttggttc gtatccttga aaaaggaaaa
     7741 cagaagagtc atgatttaaa aaccattcaa aaacatatca ctactatcga gaaagagctt
     7801 ggaattgagt atgataacat tcaaaaacag gctatctgtg atgctatcca gaataaggtc
     7861 tttatcctga caggtggacc tggtactggt aagacaactg ttatcaatgg gatcatcgct
     7921 gtttacgccc tgttagaggg acttgacctc aggaagaaaa gcaacctgcc cattcttctt
     7981 gctgctccaa ctggtcgagc tgctcgtcgc atgaatgaat tgacaggttt gcctagcgcg
     8041 accatacacc gccacttggg aatgacaggt gacgatgata ccagtcatct ggaagattac
     8101 ctagatgctg actttatcat cgtggatgaa ttctctatgg tggatacttg gctggccaat
     8161 caactcttct ccaatatctc ttctaacagt aagatcctca tcgtaggtga cagtgatcag
     8221 ctaccttctg tcagtcctgg acaggttcta gcggatctgc ttcatattcc tttgattcct
     8281 cagactcgct tggaaaaaat ttatcgacaa agcgaagaat caaccatcgt caccctagct
     8341 agtcagattc gacagggcat cttgccagct gatttcaccc aaaaaaaagc tgaccgttcc
     8401 tactttgaaa ttgctagtgg ccatattcct gccaccattg aaaaaatctt aggtgccgcc
     8461 ctcagaaatg gtattcctgc ccgtgatatc caagttctgg ctcctatgta ccgagggacg
     8521 gcagggattg atgctatcaa tcagctcatg caagacctgc tcaatccccc acaaaaagat
     8581 caactcagtt ttgaagctcc tcagtgccac tatcgtaagg gagacaaggt cattcatttg
     8641 gttaacgatg ctgaaatcaa tgtctttaat ggggatttag gagctatcac agacctgatt
     8701 cctggtaaat acaccgaatc gaaacaagac gagattgtca ttgattttga tggtaatgaa
     8761 gtctcttacc cccgtaacga atggtacaag attcgcttgg cctatgccat gagtattcat
     8821 aagtctcagg gaagtgagtt cccagttgtc atcctaccga ttactagtgc tagccgacgt
     8881 atgctggagc gaaatctcat ctacacagcc attacacgtg ccaaaagcaa actcatctta
     8941 ctaggcgaat tacaagcctt cgactatgct acccaacaca tcggaactgc ccgaaaaacc
     9001 tatctgattg aacgcttcag tgatctactg gagaatgttg aagaaaagca acaagctgtc
     9061 tctgaaacag tcacatcaag tgcctctgaa caatcctaca tcctaaccga agaaaactgg
     9121 gacagcattc cagccatgat tgggattaca gacacagacc tcaaagagat ttttggaaaa
     9181 tagtgcatca gaaaaaccac caattcaggt gggttttctg tttattaaga ttattttact
     9241 gttgctgtgc ttgtgatcaa ttcaacagct tttttgatag tgatatcatg tttcaacatg
     9301 tcagctgaaa gcaagttttg aacttgtgca acttccatgt tgtagtctgc tgccaattgc
     9361 tcaacttctt tttggatttc ttcttctgaa gcatcaaatc cttcagcttt ggcaactgct
     9421 tcgataacaa ggttagtctt agtacgtgac tcagcttctg cttggtattg gttgtgaagg
     9481 tcttcttgag tagttccagt gatttggaag tacatgtcag ggttgatccc ttgacgttgc
     9541 aaattcccaa ggaattcatt tactgaacgg tgaacttctt catggatcat ttcttctgga
     9601 agttctacga tttcagcatt ttctacagct gtatcaattg ctgcaccttc aactgcatct
     9661 ttgtaagctt cttctttagc agcagccaat tctttgcggt atttttcttt caagtcagca
     9721 agtgtttcaa cttcttcatc aatgtctttt gcaagttcat cgtcaagagc tggaacttct
     9781 ttagctttta cttcgtggat agttgtcacg aatttagctt ctttacctgc aaggtcttct
     9841 gcttggtagt cttctgggaa tgttacgata acatcaacgg tttcgccagc tgagtgacct
     9901 accaattggt cttcgaaacc agggatgaat tgacctgaac caagtccaag tgagaagttt
     9961 tcaccttttc caccgtcaaa ttcaacaccg tcgatagaac caacgaagtc gatcacaaca
    10021 gtgtcgccgt tttcagcagc agcttccttg ataaccaatt cagccaggtt gttgcgttcg
    10081 cgttcgatac gctcttcgac atcagcgtca gttacttctt tttctacatc aactgatact
    10141 tcaaggtttt tgtagtcacc caattttact tcaggttttg taacgacttc agcagtgata
    10201 acccagtctt gacctttttc cattgaagtt acgtcaattt ttggttgggc aaccacttca
    10261 agaccagctt cttttacagc tgcttcataa gcgtttggca aaagagcgtt cataacgtct
    10321 tggtaaagtg attcttcacc aaatttttgg tcgaagatag gacgtggaag gtgaccttta
    10381 cggaaacctg gaacattaag agatttcttc actgacttga agacacggtc caattctggt
    10441 ttgatttggt cttgagagat agtgaaagtc aagacaccac ggtttgtttc tttgttttca
    10501 aatgatacag acattctgtc atttctcctt aaaatttt
//
LOCUS       DAVHEW010000075        10375 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVHEW010000075 DAVHEW010000000
VERSION     DAVHEW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.075"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..180
                     /locus_tag="VVL53_001624"
     CDS             <1..180
                     /locus_tag="VVL53_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4183773.1"
                     /translation="YENRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDTSSSDV
                     TLITGKNGTGKSTLL"
     gene            complement(546..1856)
                     /locus_tag="VVL53_001625"
     CDS             complement(546..1856)
                     /locus_tag="VVL53_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU4183774.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2093..2965
                     /locus_tag="VVL53_001626"
     CDS             2093..2965
                     /locus_tag="VVL53_001626"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU4183775.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3096..3942
                     /locus_tag="VVL53_001627"
                     /pseudo
     CDS             3096..3942
                     /locus_tag="VVL53_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4003..4965
                     /locus_tag="VVL53_001628"
     CDS             4003..4965
                     /locus_tag="VVL53_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4183776.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            5142..6149
                     /gene="gap"
                     /locus_tag="VVL53_001629"
     CDS             5142..6149
                     /gene="gap"
                     /locus_tag="VVL53_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4183777.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(6217..7053)
                     /locus_tag="VVL53_001630"
     CDS             complement(6217..7053)
                     /locus_tag="VVL53_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4183778.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            7179..9374
                     /locus_tag="VVL53_001631"
     CDS             7179..9374
                     /locus_tag="VVL53_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU4183779.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETKAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9427..9579
                     /gene="rpmG"
                     /locus_tag="VVL53_001632"
     CDS             9427..9579
                     /gene="rpmG"
                     /locus_tag="VVL53_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4183780.1"
                     /translation="MALKKASLACAVCGSRNYSIKISENLKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9589..9765
                     /gene="secE"
                     /locus_tag="VVL53_001633"
     CDS             9589..9765
                     /gene="secE"
                     /locus_tag="VVL53_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU4183781.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9820..10356
                     /gene="nusG"
                     /locus_tag="VVL53_001634"
     CDS             9820..10356
                     /gene="nusG"
                     /locus_tag="VVL53_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU4183782.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         3249 a         1803 c         2252 g         3071 t
ORIGIN      
        1 tatgaaaaca gaactgtttc ttttgctatt agttcaaaag gagaaaaaat gaaagtagaa
       61 aatatttcgt atagggtgga tcatcgtaaa ttgtttgata atatttcttt tgatacttcg
      121 agttcagacg tgacattaat tactggtaaa aatggtacag gaaagtcaac tttactatag
      181 tagattgaaa ctagaatagt acacatctac ttctaaaata ttgttagaaa tcgatttgac
      241 tatcctgatc tatttgtcct gttcttattt catttcacta tatctcaaat tgagtatgac
      301 gaagtgcgct cccatgtcct gggaacgcac tttcttcata tttttcatat tcttgaatcc
      361 atcgataaag actattggga tgaattttta aagttgaact aatcattttt acaggatgag
      421 atttacagca gagagtttga aggctttatc aaaggttttt cttggcataa tgacttttcc
      481 tcgtttccac ttaattttgt gtctacttta ttataccaag tccaccctta agttagataa
      541 taaatctaac ttaaggaagc tagaaggatg agaatccagg tggtcaagag tcccaaactt
      601 aagctgatgg ggacacccag aataatttgc tttttgaagg caaggccacg ttcctctata
      661 ttgggaagtg agagttgaat gagagaacca gctgatgaaa agggtgagat attagtagat
      721 agagcgccaa taacggtggc tgttgtgagt aagtgaatat caatctgagg attttgagca
      781 ctgatgatag caatgatggg aaagagggct ggagctacaa cggatagggt ggaactaaag
      841 agtgacatca ctccggctat cacacaaaag aacagaggta accagaaatg aggaatggtt
      901 gttgtcatga ggtgccctat cagtgtgact aaacctgact tgaccgctag agacattagt
      961 aagctcatgc cgcagagcat gataattgta gcccagggaa ccttagctaa aatggcttct
     1021 tgcttcccta atttgagcct taaggcgagg cagaccatga gtattgagac aaagccaata
     1081 tcaaatgttt tttgataagt agctatccag gcgatgtttg ggaaaatgag atgcaacaag
     1141 ggaaaaagcc aaaccaaaac catgctgctg atcatgagca aggtggtttg tctttgaacc
     1201 ttgctgagga gtggtggttg gtcaatagtc aaggatgagt ttgttcttcc cttactatag
     1261 tgactgtaac aggataataa aagcaagacg atgagtgggt agataatgct gacgataaag
     1321 atatgattgc caagtgaaaa agcttgctct tcccatccca tttgcttaaa caggccttga
     1381 aagacaatgc ctgagctact ggttatcaaa ttagcccctc ctgaagctcc ccaattgacg
     1441 gcttgagctc caatcaaagg gtgtttgtcc gctttttgac agagggtaat cgctagagga
     1501 cagcaaacgg ccatagtagt gaaaaatcca gcacctaaag cagacaaaag ggttgccatc
     1561 aggtataaaa tcatgtagag ggcgttaggg tgggtgcgtg tgcggtagag aatgtgttga
     1621 gccaaaacat caagagtacc gttagttgtt gcaacgttat aaaagagaga gacgctaaaa
     1681 atggtaaaaa agagtgaggt tggccaaaaa tgaagaagtt ctttggggct taatcccatg
     1741 agagtggttg cgatgaggta agaaaaagca atagccagca ggccaatatt gattttggtg
     1801 cggtaaccaa ttccaatggc tagagcaatg gcgctaatca ttattaaatg aatcattgaa
     1861 ttgtcctttc agttagaata tggaaatagg ttggcaattt tggcgctcaa aagaccacct
     1921 gtttcctatt ggtacttgac tattatacac aaaaaaaata caattatctt gacaattgta
     1981 ttgacacttg ttagaattaa aaatgaggta ttcaggcttg tgatttccta tggaagttaa
     2041 tagtgattgc ctctaatgct tacaagtgat attaaaaata gaggacctag tgatgtcaat
     2101 catttcaact gatttaaccc cttttcaaat agatgataca ttgaaagcag ccttgcgaga
     2161 agatgttcat tccgaagatt acagtaccaa tgccattttt gatcatcatg gccaagccaa
     2221 ggtgtcgctt tttgccaagg aagctggtgt tttagcgggg ctaaccgttt ttcaaagggt
     2281 ttttacccta tttgatgccg aggtgacctt ccagaatcct catcaattta aggatgggga
     2341 tcgtttgact agtggcgatt tggttttaga aatcataggc tcggtgagaa gtctcttaac
     2401 atgtgaacgc gttgccttga attttttaca acatttatca gggattgctt cgatgacagc
     2461 tgcttatgta gaagccttag gcgatgattg cattaaggta tttgatactc gaaaaactac
     2521 tcctaattta cgtctttttg agaaatatgc cgtgagagtt ggcggtggct ataatcatcg
     2581 ctttaattta tcagatgcta tcctgctaaa agacaatcac attgcggcag taggtagtgt
     2641 tcaaaaggca attgctcaag cgcgtgccta tgcccctttt gtgaaaatgg tcgaggtgga
     2701 agtggaaagc cttgctgctg ccgaagaagc tgcggcggcg ggtgctgata ttatcatgtt
     2761 ggataatatg tcattggaac agattgaaca ggccattacc ctaattgcag gacgttctcg
     2821 gattgaatgt tctggaaata ttgatatgac cactattagc cgttttcgtg gtttagcgat
     2881 tgattacgtc tccagtggta gtttaaccca tagtgctaag agtcttgatt tttccatgaa
     2941 ggggttaacc taccttgatg tctaagttgt aaaataaact aactttttaa aggatgtctt
     3001 tcctctagaa cgagttttat gtcagatagt ttaaacgcct cttcaaatat agtaaaatga
     3061 accaaaaata gtacacaatg tggtataatc tttttatggc atattcaata gattttcgta
     3121 aaaaagttct ctcttattgt gagcgaacag gtagtataac agaagcatca cacgttttcc
     3181 aaatctcacg taataccatt tatggctggt taaagctaaa agagaaaaca ggagagctaa
     3241 accaccaagt aaaaggaata aaaccaagaa aggttgatag agatagactt aaaaactatc
     3301 ttactgacaa tccagacgct tatttgactg aaatagcttc tgaatttggc tgtcatccaa
     3361 ctaccatcca ctatgcgctc aaagctatgg gctacactcg aaaaaaagaa ccacacctac
     3421 tatgaacaag acccagaaaa agtagcctta tttcttaaga attttaatag tttaaagcac
     3481 ctaacacctg tttagattga cgaaacagga ttcgatactt atttttatcg agaatatggt
     3541 cgctcattaa aaggtcagtt aataagaggc aaagtatctg gaagaagata tcagaggatt
     3601 tctttggttg caggtctaac aaatggtgaa ttaatcgctc caatgactta cgaagagacg
     3661 atgacgagcg acttttttga agcttggttt cagaattttc tcttaccaac attaaacaca
     3721 ccatcggtta ttattatgga taatgtaaga ttccatagaa tggggaagct agaactttta
     3781 tgcgaagagt ttgggcataa acttttacct cttcctccct actcgcctga gtacaatcct
     3841 attgagaaaa catgggctca tatcaaaaag cacctcaaaa aggtattacc aagttgcaat
     3901 accttttacg aggctttttt atcctgctct tgtttcaatt gactatatat cacggataca
     3961 gttgggaaag aagctattaa atatttggag agtccttttg ggatgaacat gtcaaatatt
     4021 actatagatg ggcaagaaat ggatctgagt caaacgatgc cttcttatgc tcaagtagtg
     4081 agtctttatg atgatacaag tatctctgtt agtaataatg agacggacaa agtgttggct
     4141 ggatccctct atactgatac aaatgaacag ggattaacga ttccaagcag tttactaaaa
     4201 aattggaatg aacagacagg aaaaaatttg acagctaatg atcttattgg caaatcagtc
     4261 tcagccagca ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt tcaaacgaag
     4321 attgtacgtg taatcaatga tgaagatgac atggaggaca gcaacagttt catgctgtct
     4381 catcaaatgg aaacgatttt gaaagaggct ggatttacga aagctgtatc ttattttatc
     4441 ttggaactca aagatccatc acagacaaaa gtagtaacag aagaattaca gaaaaataag
     4501 aagtatactg tgctttctca acagagagtt cttgatattg tgattacctt tattcgtgtt
     4561 attcagggat tattgattgt gctttcatca caagctattg tggtagcagc ggttatgatt
     4621 ggtatcatta tttacatcaa tatcatgcaa cgttccaagg aaataggtgt catgaaagca
     4681 gttggttatc agaatcgtga tgtcaaagga atttttattt acgaggctat ctggattgta
     4741 ggcatcgcct tgctgctggc atttttggtt gcacaagggg tgggaagttt ggcgaatgcg
     4801 attgtaagtc acttttaccc atccatcact aaggtttttg aattaaatct tttatctgtt
     4861 ttaggaactc tagttttcgc tctattactt ggttatgtct cagcctactt cccggcgcgt
     4921 aagattagta aaatggatcc tatagaatcg ttatgctatg aatagtaagg gattccttgg
     4981 tttacagtct taaaaagaaa ctaacataga aaaacaatgg ccatacttgc aattcaactc
     5041 aaatagttgt ataataggtt tgttgaaagg tgatttgtag tgattcaagt tactcttttc
     5101 acaataaaaa tttcatgatt ttcataagga ggaaatcact aatggtagtt aaagttggta
     5161 ttaacggttt cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac gtagaaggtg
     5221 ttgaagttac acgcatcaac gaccttacag atccagttat gcttgcacac ttgttgaaat
     5281 acgacacaac tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt ggatttgaag
     5341 ttaacggtaa attcatcaaa gtttctgctg aacgtgatcc agaacaaatc gactgggcta
     5401 ctgacggtgt agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa gaagcagctg
     5461 aaaaacacct taaaggtgga gctaaaaaag ttgttatcac tgctcctggt ggaaacgacg
     5521 ttaaaacagt tgtattcaac actaaccacg acgttcttga cggtactgaa acagttatct
     5581 caggtgcttc atgtactaca aactgcttgg ctccaatggc taaagctctt caagacaact
     5641 ttggtgttgt tgaaggattg atgactacta tccacgctta cactggtgac caaatgatcc
     5701 ttgacggacc acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct gcaaacatcg
     5761 ttcctaactc aactggtgct gcaaaagcta tcggtcttgt aatcccagaa ttgaatggta
     5821 aacttgacgg atctgcacaa cgcgttccaa ctccaactgg atcagttact gaattggtag
     5881 cagttcttga aaagaacgtt actgttgatg aagtgaacgc agctatgaaa gcagcttcaa
     5941 acgaatcata cggttacaca gaagatccaa tcgtatcttc agatatcgta ggtatgtctt
     6001 acggttcatt gtttgacgca actcaaacta aagttcttga cgttgacggt aaacaattgg
     6061 ttaaagttgt atcatggtac gacaacgaaa tgtcatacac tgcacaactt gttcgtactc
     6121 ttgaatactt cgcaaaaatt gctaaataat tcttgagttg atagaaagca aggctttgtg
     6181 gtcttgcttt tttatatgga aaaatggatg acacgatcat ccattctttt ttaattcttt
     6241 ttcaaatgta tttgaaaggg tagtgaaagt tagcttctct aaagtaagtg ggtgggtaaa
     6301 ggaaagtcgg aaggcatgaa gcataagccg gcttgtcttt gatttactat tatagagagg
     6361 gtctcccagg ataggaagat tatgatgcga aaggtgcaca cgaatctgat gggttcgccc
     6421 tgtctttagc ttgcaatgag ccaaggaagt cttgtttgag aattgcttta atctgcttac
     6481 atgcgtttca gcatattgcc cattttttgc atcaactatt ctttttctac gatcatggcg
     6541 atcacgtcca attttgtctc tgaaaacaag ttcttttctg ttgatatttc catcaactag
     6601 agcccaatat tctctagaaa tctctttttt ctccaataag cgattgagaa tgggcaggat
     6661 aaaaggattt ttggcaaaga gaactaagcc actggtttcc atgtccagac gatgaacgac
     6721 atagcaggtt tggccaacat aggtactgac atggttaaga agggcaattt cgtttggttg
     6781 attaccatgc gttttcatcc cctctggttt gtttacaata atcaagtgtt gatcttgata
     6841 aacttcctgc actaagtctg ggttgcccca agggatcgtc ttttgggaat aatcttcctc
     6901 gtcaaaagtc aactggcaaa catctccagg atttacgatt tccttccagt ggacttcttc
     6961 ttgatttatc aaaatatgtt tcttgattct caaaaaatga cggatttttc tagggatgag
     7021 gagttgttcc tcaagtaatt gctttactgt catttgaggt agagaggcgg gtaatgtaaa
     7081 tgtgaattgc atacagatat tgtaacaaaa aaagccctat ttggatagga aatagctaaa
     7141 ttcttgtctt cctatgatga agatgataaa ataaacgcat gaaattagat aaattatttg
     7201 agaaatttct ttctcttttt aaaaaagaaa caagtgaact agaggactct gattctacta
     7261 tcttacgtcg ctctcgtagt gatcgaaaaa aattagccca agtaggtccg attcgaaaat
     7321 tctggcgtcg ttatcatcta acaaagatta tccttatact aggtttgagt gcaggcttgc
     7381 tagttggaat ctatttgttt gctgtagcca agtcgaccaa tgtcaatgat ttgcaaaatg
     7441 ccttgaaaac tcggactctt atttttgacc gtgaagaaaa agaggctggt gccttgtctg
     7501 gtcaaaaggg aacctatgtt gagctgactg acatcagtaa aaacttgcag aatgctgtta
     7561 ttgcgacaga agaccgttct ttctataaaa atgacgggat taactatggc cgttttttct
     7621 tggctattgt cactgctgga cgttcaggtg gtggctctac cattacccaa cagctggcta
     7681 aaaacgccta tttatcgcag gatcaaactg ttgagagaaa agcgaaagaa tttttccttg
     7741 ccttagaatt aagcaaaaaa tatagtaagg agcaaattct aaccatgtac cttaacaacg
     7801 cttattttgg aaatggtgtg tggggtgtag aagatgcgag taagaaatac tttggagttt
     7861 ctgcatcaga agtgagtctg gatcaagctg cgactctggc agggatgctc aaggggccgg
     7921 aactgtataa tcccttgaat tccgtagaag attctactaa tcggcgcgat actgtcttgc
     7981 agaatatggt tgcagcagga tatattgata aaaaccaaga aaccaaagct gctgaagttg
     8041 atatgacttc gcaattgcac gataagtatg aaggaaaaat ctcagattac cgttacccct
     8101 cttattttga tgcggtggtt aatgaagctg tttccaagta taatctaaca gaggaagaga
     8161 ttgtcaataa tggctaccgc atttacacag agctggacca aaactaccaa gcaaatatgc
     8221 agattgttta tgaaaacaca tcgctatttc cgagggcaga ggatggaacg tttgctcaat
     8281 caggaagtgt agccctcgaa ccgaaaacag ggggagttcg tggagttgtc ggtcaagttg
     8341 ctgacaatga taaaactgga ttccggaatt tcaactatgc aacccaatca aagcgtagtc
     8401 ctggttctac aattaagcct ttagttgttt atacgccagc agttgaagca agctgggctt
     8461 tgaataagca gttggataac cataccatgc agtatgacag ctataaggtt gataactatg
     8521 cagggatcaa aacgagtcga gaagttccta tgtatcaagc cttggcagaa tcgcttaatc
     8581 tacctgctgt tgccactgtt aatgatttgg gtgtcgacaa ggcttttgag gcaggcgaaa
     8641 aattcggact caacatggaa aaggtcgacc gtgttcttgg tgtcgccttg ggaagcggtg
     8701 ttgaaaccaa ccctcttcaa atggctcaag catacgccgc ctttgcaaat gaaggtttaa
     8761 tgcctgaagc tcattttatt agtagaattg aaaatgctag tggacaagtt attgcgagtc
     8821 ataaaaattc acaaaaacgg gtgattgata agtctgtagc tgacaagatg accagtatga
     8881 tgttggggac tttcaccaac ggtaccggta ttagttcatc gcctgcagac tatgtcatgg
     8941 cagggaaaac tggaacaact gaagcagttt tcaatccgga gtacacaagt gaccagtggg
     9001 taattggtta tactccggat gtagtgatta gccactggct tggctttccg accactgatg
     9061 aaaaccacta tctagctggc tctacttcaa acggtgcagc tcatgtcttt agaaacattg
     9121 ccaataccat tttaccttat acgccaggaa gtacctttac ggttgaaaat gcttataagc
     9181 aaaatggaat tgcaccagcc aatacaaaaa gacaagtaca aaccaatgat aatagccaga
     9241 cagatgataa tttgtctgat attcgagggc gtgcgcaaag tctagtagat gaggctagcc
     9301 gggctatctc agatgcgaag attaaggaaa aggctcaaac aatatgggat tcgatagtca
     9361 atctatttcg ctaagatgct tgtcaaagcc tagctttctt gttataatgg ataagatgga
     9421 ggcgttatgg cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc gagaaactat
     9481 tcaatcaaga tcagcgaaaa cctcaagcct acacgactag aagtaaataa attttgtaag
     9541 cattgtggca agtacactac acacagagaa acgagatagg agagagcgat gcgttttatt
     9601 ggagatattt ttagacttct taaagacaca acatggccaa ctcgcaagga aagctggaga
     9661 gattttcgtt ctatcatgga atacacagct ttctttgtag taattattta catttttgac
     9721 cagttgattg tttcaggttt gattcgattt attaacattt tttagaagat tagtggagtt
     9781 aattacacta gaaatcttct atttatgaaa ggaaatatca tggatagttt tgataaaggg
     9841 tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa tctattacaa
     9901 cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc aacacaaaca
     9961 gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt tccaggttat
    10021 gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa cacaccaaat
    10081 gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt attggaacaa
    10141 gaaattcgcg acattttggt atctatggga caaactgttc aagaatttga tttcgatgtt
    10201 gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac tggtaagatt
    10261 acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa tgacacagtt
    10321 gcagaagtaa atctaaacca aatcgcagaa ttataaccct gagagaggct ggagc
//
LOCUS       DAVHEW010000076        10021 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVHEW010000076 DAVHEW010000000
VERSION     DAVHEW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.076"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            72..842
                     /gene="sufC"
                     /locus_tag="VVL53_001635"
     CDS             72..842
                     /gene="sufC"
                     /locus_tag="VVL53_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU4183783.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            870..2132
                     /gene="sufD"
                     /locus_tag="VVL53_001636"
     CDS             870..2132
                     /gene="sufD"
                     /locus_tag="VVL53_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU4183784.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIECVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIVGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            2143..3369
                     /locus_tag="VVL53_001637"
     CDS             2143..3369
                     /locus_tag="VVL53_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4183785.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            3356..3796
                     /locus_tag="VVL53_001638"
     CDS             3356..3796
                     /locus_tag="VVL53_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU4183786.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            3850..5262
                     /gene="sufB"
                     /locus_tag="VVL53_001639"
     CDS             3850..5262
                     /gene="sufB"
                     /locus_tag="VVL53_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU4183787.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(5461..6702)
                     /locus_tag="VVL53_001640"
     CDS             complement(5461..6702)
                     /locus_tag="VVL53_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU4183788.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            6805..7914
                     /locus_tag="VVL53_001641"
     CDS             6805..7914
                     /locus_tag="VVL53_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4183789.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            8560..8907
                     /gene="ssrA"
                     /locus_tag="VVL53_001642"
     tmRNA           8560..8907
                     /gene="ssrA"
                     /locus_tag="VVL53_001642"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9090..9177)
                     /locus_tag="VVL53_001643"
     tRNA            complement(9090..9177)
                     /locus_tag="VVL53_001643"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9141..9143),aa:Ser,seq:gga)
     gene            complement(9267..>10021)
                     /locus_tag="VVL53_001644"
     CDS             complement(9267..>10021)
                     /locus_tag="VVL53_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU4183790.1"
                     /translation="ADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQKFSPEMMVN
                     KKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGKSIEERPDVI
                     NLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGE
                     HRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRRWLPKGTKKT
                     TPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT         2962 a         1837 c         2110 g         3112 t
ORIGIN      
        1 ctctatttct ggggaaatca gacgtttttc tagtaagtac tgtaaaagtt ttgaaaaaga
       61 aaggaactat catgtcagta ttagagatca aagatcttca cgttgagatt gaaggaaaag
      121 aaattttaaa aggggttaac ctgaccctga aaacaggaga aattgccgct atcatgggac
      181 caaatggtac aggtaaatcg actctttctg ccgctatcat gggaaatcca aactatgaag
      241 taactaaagg tgaagttttg tttgatggcg taaacatcct tgagttggaa gtggatgagc
      301 gtgcgcgtat gggacttttc cttgctatgc aatacccatc agaaatccct ggaattacca
      361 atgctgagtt tcttcgtgcc gctatgaatg cgggtaaaga agatgatgag aagatttcag
      421 ttcgtgagtt tattactaag ctagatgaaa aaatggaatt gctcaacatg aaagaagaaa
      481 tggcagagcg ttacctcaac gaaggcttct ctggtggtga gaaaaaacgc aatgaaattc
      541 ttcaactttt gatgttggag ccaacatttg ctcttttgga cgagattgac tcaggtcttg
      601 atattgacgc tcttaaagtt gtgtctaaag gtgtcaatgc catgcgtggt gaaggttttg
      661 gtgctatgat catcactcac taccaacgtc ttttgaacta tatcacacct gatgtggtac
      721 acgtgatgat ggaaggtcgt gttgtccttt ctggtggtcc agaattggct gcgcgtttgg
      781 aacgtgaagg atacgcaaaa ttagctgaag aacttggcta cgactacaag gaagaattgt
      841 aattccctcg tatcttttag gagaagtaaa tgactagaga aaatattaaa cttttttcag
      901 aaatgcacgc tgaaccaagc tggttggctg atctccgtca aaaagctttt gacaagattg
      961 agactttgga attaccagtt attgagtgtg tcaaattcca ccgttggaat ctgggtgatg
     1021 gaacgattac agaaaatgag ccatcagcaa atgttccaga tttcacagct ttagatcatc
     1081 acttgaagtt ggtgcaagta ggaactcaaa ctgttttcga acaaactcca gttgagttag
     1141 ctgaacaggg tgttgtcttc acagactttc actcagcttt agaagaaatt ccagagctga
     1201 tcgaagaatt cttcatgtca tctgttaagt atgatgatga caagttggcg gcttaccaca
     1261 cagcttactt taacagtggt gctgtactct atattccaga taacgtagaa atcacagagc
     1321 caattgaagg aattttctac caagatagcg atagcaatgt gccgtttaac aagcatatta
     1381 tgattatcgt tggtaaaaat tctaagatta gttatctgga gcgtttagag tcacgcggtg
     1441 aaggaagtga caaagcaact gccaatatca cagtggaagt gattgcacgt tctggtgcgc
     1501 aagtcaagtt tgctgctatc gaccgtctag gtgaaaacgt cactgcctac attagccgtc
     1561 gtggtaaatt aggcaacgat gcaagtattg actgggctat cggtgtcatg aacgaaggaa
     1621 atgtcgttgc tgattttgat agtgacttga ttggtaatgg tagccatgct gacctcaagg
     1681 ttgtagctct ttcaagtggt cgtcaggtac aagggattga tactcgtgta actaactatg
     1741 gctgcaactc aatcggaaac attctacaac atggggttat ccttgaaaaa gcaactttga
     1801 ctttcaatgg tatcggccac atcatcaagg gtgctaaggg agcagatgcg caacaagaga
     1861 gccgtgttct catgctttca gaccaagcgc gttcagatgc taacccaatt cttttgattg
     1921 atgaaaatga cgtaactgca ggccatgcag cctctattgg tcaggtagat ccagaagata
     1981 tgtactacct catgagtcgt ggcttggata aggcaactgc agagcgtttg gttgttcgtg
     2041 gtttccttgg atctgttatc gtggagattc cagtcaagga agttcgtgat gaaatgattg
     2101 caactatcga agagaaattg tcaaaacgct aaggggcagc ctatgttaga tgtagaagcg
     2161 attcgcaagg attttccaat tttagatcag attgtcaatg atgaacctct ggtctatctg
     2221 gacaatgctg cgacgacaca aaaaccacta gtagttctga aagctattaa cagctactat
     2281 gagcaggaca atgccaatgt tcaccgtggt gtccatacct tagcggaacg agcgacagct
     2341 tcttatgaag ctgctcgtga aaccattcgt aagtttatta atgcaggctc tacaaaggaa
     2401 gttctcttta ccagaggaac gacaaccagc cttaactggg tggcacgctt tgctgaggaa
     2461 attctcactg agggagacca ggtcttgatt tcagtaatgg aacaccattc taatatcatt
     2521 ccatggcagg aagcttgtcg aaagactgga gcagagcttg tctatgtcta tcttaaagac
     2581 ggtgccttgg atatggagga tttgcgagct aaattgactg ataaggttaa atttgtttcc
     2641 ctagctcatg cctccaatgt tcttggtgtg gtcaatccga tcaaggaaat cactcaatta
     2701 gcccaccaag ttggggcaat tatggtagtg gatggtgctc aatctacacc tcatatgaag
     2761 attgatgtcc aggacttgga tctggacttt ttcgcctttt cgggtcacaa gatggctggt
     2821 ccgactggta tcggtgtcct ttacggcaaa gaaaagtatc ttgagcaaat gtctccagta
     2881 gaatttggcg gcgagatgat tgattttgtc tacgagcaat ttgctagttg gaaggaattg
     2941 ccttggaaat ttgaggctgg aacgccaaat atggcaggag ctattggact tgcgactgca
     3001 gttgattatc tggaaaagat tggtatggat gccgttgaag ctcatgaaca ggaattgatt
     3061 gcgtacgtct atccaaaact gcaggcaatt gagggattga ccatttacgg ttctcaggat
     3121 ttggctcaac gttcgggtgt tattgccttt aacctaggtg atctccatcc tcacgatctt
     3181 gcgacagctc tggattatga aggagtggct gttcgtgctg gtcaccattg tgcgcaaccc
     3241 ttgcttcagt atttggaagt cccagcaaca gctcgtgcaa gtttttatat ctataatacc
     3301 aaggcagatt gcgacaaact agtcgatgcc ctacaaaaga caaaggagtt tttcaatggc
     3361 actttctaaa ctagatagcc tttatatggc agtggtagca gaccattcga aaaatccaca
     3421 tcaccaaggg aagttagaag acgctgagca aatcagtctc aacaatccga cttgtggaga
     3481 tgtcatcaat ctctctgtca agtttgatgc agaggaccgt ttggaagata ttgcttttct
     3541 aaattcagga tgcacgattt caactgcttc tgctagtatg atgacagatg ccgttttagg
     3601 aaaaaccaaa caagaaattt tagaactggc gactattttt tctgaaatgg ttcaagggca
     3661 aaaagatgag cgtcaagacc aacttggaga cgcggcattc ttgtcaggtg ttgccaaatt
     3721 ccctcaaaga atcaagtgtg caaccctagc ttggaatgcc cttaagaaaa caattgaaaa
     3781 tcaagaaaaa cagtaagaca agtttctttt gtcttatgaa ttattagaaa tgaagaaaga
     3841 aaggatatta tggctgaaga aagagtagaa ccaaaaccaa ttgaccttgg tgaatataaa
     3901 tttggtttcc atgacgatgt agagcctgtc ttatcgacag gaaaaggact caacgaaggt
     3961 gttattcgtg aattatctgc tgctaagggt gagcctgagt ggatgttgga gttccgtttg
     4021 aagtcttatg aaaccttcaa aaaaatgccc atgcaaactt ggggagcaga cttgtcagag
     4081 attgactttg atgacttaat ctactaccaa aaaccatctg acaaaccagc ccgttcttgg
     4141 gatgatgtac ctgaaaagat taaagaaacc tttgaacgta tcgggattcc agaagctgaa
     4201 cgtgcttatt tagcaggggc ttctgcccag tacgagtcag aagtggttta ccacaacatg
     4261 aaggaagagt tccaaaaatt aggtattatc tttacagata cagattccgc actcaaggaa
     4321 tacccagact tatttaatca atactttgcg aagttggtac cgccgacaga taacaagttg
     4381 gcagccctca actcagcagt atggtcgggt ggaactttta tctacgtgcc aaaaggtgtc
     4441 aaggtagata ttccacttca aacttatttc cgtatcaata acgaaaatat aggtcagttc
     4501 gaacgtacct tgattatcgt tgatgaggga gcaagcgtcc actacgtaga aggatgtaca
     4561 gcaccaacat attcaagcaa tagcttacac gctgccattg tagaaatttt tgctttggac
     4621 ggagcttata tgcgttatac aactatccaa aactggtctg ataacgtcta taacttggta
     4681 acaaagcgtg ctaaggctca aaaggatgcc actgttgagt ggattgatgg aaacttgggt
     4741 gccaaaacga ctatgaaata tccatctgtt taccttgatg gagaaggagc gcgtggtacc
     4801 atgctctcta tcgcctttgc taatgcaggg caacaccaag acacgggtgc taagatgatt
     4861 cacaatgctc cacataccag ctcgtctatt gtgtctaaat ccatcgctaa aggtggagga
     4921 aaggttgact accgtggaca agtcaccttt aacaagaact ctaagaaatc tgtttcccac
     4981 attgaatgtg ataccattat catggatgac ttgtcagcat cagatactat tccatttaat
     5041 gaaattcaca actcgcaagt ggctttggaa cacgaagcca aagtatctaa gatttcagaa
     5101 gagcaattgt attatctcat gagccgtgga ttgtcagaat ctgaggcaac tgaaatgatt
     5161 gtcatgggat ttgtagaacc ctttacaaaa gaacttccaa tggaatacgc agttgagctg
     5221 aaccgcttga ttagctatga aatggaggga tcagttggat aaaatttgat tttatactct
     5281 tcgaaaatct cttcaaacca cgtcagcatc gccttaccgt atgtatggtt actgacttcg
     5341 tcagtttcat ctacaacctc aaaacagtgt tttgagcaac ctgcggctag cttcctagtt
     5401 tgttctttga ttttgaagat gaacttgtat caaaaaatcg cggtttaaaa tcgcgatttt
     5461 ttataatttc tcgttaacaa aacggacaaa ctgattccac caaactttta agaagaaggc
     5521 tttttcaatt ttcttgtctg ctaccatttc gaaactaggg cgctctgtgg tgatgtaacc
     5581 ttgaccaatc aagtccttgt cttcataagt caaatggcca accactgttc cagcttcaag
     5641 tggtgctggg attgctttgg aatcaggtgt gaattgaaca gattgggaag attgattccc
     5701 aacacgttcg attagataga tatcctctgg agccactgca gttactgtat cttcttttcc
     5761 atcttgtaca ggggctttgc tatcttgata ggcatcgcct tgttgaacga ttttgcgaag
     5821 tgtaaatgta gaagaaatat aatccattag ggaagatgta gctgtaaatc gagcgtaagg
     5881 attattgtct tgatgatctg catttaaaac aactgtgatg actctcatgc ctttttcgac
     5941 agtagtacca acaaaagact ctccagcctt atctgttgtt cctgttttta gcccatcaaa
     6001 accaccacgg taagcaggca taccttctaa catgtagttg gttgaagtga ttgtcatccc
     6061 agcaaaagta gaagaaggtt ttttggtgat ttctaagact tgtgggtatt ttttgatgag
     6121 gttgcgagca acgatagcga catcataagc actaagctta ttttcctcat cttttttaga
     6181 acctgggtaa atgttatccc ctagagtttc attgttaaga cctgtcgtat tgacaacagt
     6241 ggcatcctga attccccatt ccaagagttt tgcccgcatc atatcgacga aatctttttc
     6301 tgagccagca attttctcag ctagggcaat agcggcgctg ttggcactag ataccagagt
     6361 tgcttcaagc aactcttcga cagtataatt acgggcctcc ataggaatat tactggcttc
     6421 agaatttgtc gtcaattgat aaggataatc agaaatatct acaggagtgg agagggtaat
     6481 acttccgttt tccaaagctt catagaccag ataaacagta atcaattttg ttatggaagc
     6541 aatttcgaca ggttgcgttg catccttctc atagagaatt ttaccagtat ttgcctcaac
     6601 agcaatcgca tgtttagcgg caatggtaaa atcttgagca acagcagtag aagcaccccc
     6661 taaaagagag acagttaaca aagttaaaaa tatttttttc atagtagtct tattctatca
     6721 taaagaaaaa aaatattctt gctttaataa ttcatctgtt aagctttttg aaaatatggt
     6781 aaaataaagt gagggaggta actcatgttt cgtagaaata aattattttt ttggaccaca
     6841 gaaattttac tcttaaccat catcttttac ctatggagac agatggggtc tttgattaac
     6901 ccttttgtta gcgtgcttaa tacaattatg attccatttt tattaggggg ctttctttat
     6961 tatttgacaa accctattgt tactttctta aataaagtct gtaaactcaa tcgtttgctt
     7021 ggtattttaa ttaccttgtg tactttggtc tggggaatgg tcataggtgt tgtctatctc
     7081 ttacctattt tgattaatca gttatctagt ttgattatat ctagtcaaac tatttatagt
     7141 cgagtacaag acttaatcat agacttatct aattatcctg cgctccagaa tttggatgta
     7201 gaagctacaa ttcagcagtt aaacttatcc tatgtggata ttcttcaaaa tatcctaaat
     7261 agcgtatcaa atagtgtggg gagcgtcttg tcagctctta tcagtactgt tttgattttg
     7321 attatgactc cagttttttt ggtttatttc ttattagatg gacataaatt cttgcccatg
     7381 cttgaaagaa cgattctaaa gagggatcgc ttgcatattg caggcttatt aaagaattta
     7441 aatgcgacga ttgctcgcta tattagtgga gtttcgattg acgcaatcat tataggttgt
     7501 ttagcttata ttggctatag tattattggt ttaaaatatg ctttagtttt tgccattttt
     7561 tctggtgtag ccaatttaat tccttatgtg gggccaagta ttggtttgat tcctatgatc
     7621 atcgcaaata tattcactga tccccataga ctgctgattg cagtgattta tatgcttgtt
     7681 gttcagcagg tagatggcaa tatcttatat cctcgaattg taggaagtgt tatgaaggtt
     7741 catccaatca cgattttagt tttacttttg ttgtcaagca atatctatgg tgtagttgga
     7801 atgattgtcg cagtgccaac ctattctatc ttgaaagaaa tttctaagtt cttatcccgt
     7861 ttgtatgaaa atcataaaat aatgaaagaa cgagaaagag aattagctaa gtaaaagtca
     7921 ggagaaccct gatttttctt tactggaagt ggcctttaga ttagaagact gaaaataagt
     7981 taaagtctta aactaatttt cacagctaag aatagtagaa gttaatctga taaaaatcga
     8041 aaaaaccagt ggaattccgt gtcagggtaa gttccactgg ttttcatagt ctattaaagt
     8101 tcgaatgaaa cctatttata gtatattgaa actagaatag tacacctcta attctaaaac
     8161 attgttagaa atcgatttga ctgtcctgat ctattcgtcc tattcttatt tcattttact
     8221 atattttggt gcaataagtg aaaagtagtc cgaataatat aaggattgat tttatagttt
     8281 ttaaactcaa atgaattaaa ataaagagag tacgaaaatt ctcatctgaa agtattttag
     8341 aataattctc ttcgtgaatt tcttcaaaac agatagcttc atcttaggta tgtgttaatt
     8401 ggatttcgta agctctattt acaatcgtaa agttatgcac tgaaaaagtt gtagttagtc
     8461 ttccagtttg ctaaatttct cgaatagtat cttttttagg tatgtgattt ctttttgcat
     8521 ttttgagtta gataaggtat aatggtttta ttgtcttttg gggtcgttac ggattcgaca
     8581 ggcattatga ggcatatttt gcgactcgtg tggcgacgta aacgctcagt taaatataac
     8641 tgcaaaaaat aacacttctt acgctctagc tgcctaaaaa ccagcaggcg tgacccgatt
     8701 tggattgctc gtgttcaatg acaggtctta ttattagcga gatacgatta agccttgtct
     8761 agcggcttga taagagattg atagactcgc agtttctaga cttgagttat gtgtcgaggg
     8821 gctgttaaaa taatacataa cctatggttg tagacaaata tgttggcagg tgtttggacg
     8881 tgggttcgac tcccaccggc tccattattc ctttgcattc ttttgcattc cttggtaaaa
     8941 cgttgttaaa tcaacgtttt ttatttttat ctttggtatt cctttgcatt cttttgctaa
     9001 aaagggagtc acaaacagac cctattttaa aaaaaggata gaaaaaagga tacaacattt
     9061 gtcgcatcct aaaaataatc ttttttcgac ggaagacatg ggattcgaac ccacgcacgc
     9121 tattacacgc ctaccgcgtt tccaacacgg cctcttaagc ctcttgagta atcttccaat
     9181 acttactcaa atagtctacc ataaaggctc ttatcttgca ataaaaattc tagaaataag
     9241 aaaaaatccc aaatttcaag ttgaagttag ccacccaaaa gaaattcact tggcgacttg
     9301 tagtccaagc attttttagg gtagttgtta atccaatttt cgataaaagc tacttctttc
     9361 ggagtcgttt tcttggttcc tttaggtaac catctccgaa ttaatcgatt atgattttca
     9421 tttgaacctc tctcccatga agagtaagca tgtgcgtagt agatatgttc ctcaggaaat
     9481 acctcagaca atcgtttgaa ctccgaacca ttatctgcag taatggacag aatacgatgc
     9541 tcccctaata gtaacgtaag cgcctgattg acagattcag cagttttatt tggaattaac
     9601 cttataattt ggtgtctgct ccgccggtcg gttaagacta acaggcaata attttttatc
     9661 ttagtcagta ggacggtatc aatttcataa tgaccatttt ccaagcgaag attaataacg
     9721 tcaggacgtt cttcgataga tttacctgcc ggcttaaagt tcggactagc ttgcttcttg
     9781 acacctttcc tttttctggg ataaagcatg tcggccttcg tcaatcctaa atgaccatta
     9841 tgaaaccagt agtagatggt tgaaatacca actttcactt gcttcttgtt aaccatcatt
     9901 tcaggcgaaa atttttgctt atgatagtgt aagatcttct ctctgatttc ctttgttaaa
     9961 attaactttt tcaccgaccg ttttcgattg aattggtaaa cagtttgtgc gtaatcggca
    10021 g
//
LOCUS       DAVHEW010000077        10014 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVHEW010000077 DAVHEW010000000
VERSION     DAVHEW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.077"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            124..423
                     /locus_tag="VVL53_001645"
                     /pseudo
     CDS             124..423
                     /locus_tag="VVL53_001645"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(701..1471)
                     /locus_tag="VVL53_001646"
     CDS             complement(701..1471)
                     /locus_tag="VVL53_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4183791.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGYLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(1486..2334)
                     /locus_tag="VVL53_001647"
     CDS             complement(1486..2334)
                     /locus_tag="VVL53_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU4183792.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(2331..3806)
                     /locus_tag="VVL53_001648"
     CDS             complement(2331..3806)
                     /locus_tag="VVL53_001648"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU4183793.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(4129..5484)
                     /locus_tag="VVL53_001649"
     CDS             complement(4129..5484)
                     /locus_tag="VVL53_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4183794.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            complement(5494..5811)
                     /locus_tag="VVL53_001650"
     CDS             complement(5494..5811)
                     /locus_tag="VVL53_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4183795.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5828..6145)
                     /locus_tag="VVL53_001651"
     CDS             complement(5828..6145)
                     /locus_tag="VVL53_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4183796.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(6158..7843)
                     /locus_tag="VVL53_001652"
     CDS             complement(6158..7843)
                     /locus_tag="VVL53_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEU4183797.1"
                     /translation="MKNKKLILSLFILFLLIFIHAKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(7848..8744)
                     /locus_tag="VVL53_001653"
     CDS             complement(7848..8744)
                     /locus_tag="VVL53_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU4183798.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHGKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(8747..10009)
                     /locus_tag="VVL53_001654"
     CDS             complement(8747..10009)
                     /locus_tag="VVL53_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4183799.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3404 a         2153 c         1384 g         3073 t
ORIGIN      
        1 gtacaggtgc ttcaatagtt tctatagtaa aatgaaccaa aaatagtaca cgttgcggta
       61 taatgttctt gtggcatatt cattagattt tcgtaaaaag ttctctccta tttgtgagcg
      121 aacaggtata gtcaattgat tgtcagtttg aagaccaaga gtcgtaaggc aaaggatatg
      181 gcagaatcca tccaagggtg gctggcacaa tttctagtga acctctttaa atctattacg
      241 tttgactgtg gaaaagagtt ttcaaaatgg aaagatattt ccaatcatca cgatagtgag
      301 agcttttttg cgaatctagg atgtccaaga cagcggtgtc tcaacgaaca ttcaaataga
      361 cttttaaggt gtcatgactt gccaaagcag acagatttca acgaagtcag tcaagaattt
      421 taggctgggt aaaaactcaa tttcaggaga aaatgaaata aatattccca caataaaacg
      481 catagtatca aagtttttcc aacacctgat attatacatt ttttaatttt aaagactttt
      541 tcccagcctc aagaaatcac atcccaccta aatcctttgg ataccaaata cctcatcagg
      601 tattttctga gttggtaaga ctgtctagct taatttgaca aatggagggc gttaaatcca
      661 gtttttacag gaagtctatt taaatatttt cacactgacc ttacaaaata tgaaataaat
      721 tatataaagt cattcccatg acaaagaata acatcccttt aaaacagtat cttacccaat
      781 tttcaggtaa gacttttgat acaaccgtcc ccaatacccc tccgcatata gaagcaagaa
      841 aaataactgg aacaggtgct aaatgatact gatttaaacc tgtaaccaca acaattgtag
      901 ccaaatgaga cagctgggaa aagaaaataa ttgcaagaga atacatcgtt gcttctttta
      961 tcgaaataga aaaaaacacc atcaataaag atacattgag cggaccacca ccaatcccta
     1021 aaaaactaga aatcaagccc aatagaagac cacaaatcat atagaatcct atcttatcaa
     1081 attgataagt ttttttaaaa tctgttaaaa caaatgatac aagtaacatc acaaatagta
     1141 aaatcatttg aacaatagat actagccgta ctgataattg agataacaaa acttgaaaaa
     1201 taagataacc tagaaatcct cctaaaacag aagaaaagga taccgtaaaa atcaatctcc
     1261 attttatctc tttactttgt gaaaatcttt tgattgttga aataatcgcc attataaaaa
     1321 cagagaaact agaataaaaa gcaatatcac tgaccgaatg gtatccaaaa ctatccataa
     1381 gtggcttaat tataattcca ccacccatgc ctgatataga acccaatcca ctagctatta
     1441 taatgattag aaaataaata atctcaacca ctttttaagt ccatcctatt ctttcaaaca
     1501 tctaaaacct agatggctac tagtggaagt agaaatacat ccatttctag cagctactcg
     1561 gtatcgatta caataagaac aatgacataa aaaggaacct cctctgatag catactcctc
     1621 atcttgtatc ccatattttg gtaactcata gtcatcttca ttaaaactag ctaataaggt
     1681 atatctcgga ttgcgacacc attcccaaac attcccaatc atttgatata gaccaaaatc
     1741 attaggtaaa aattcataga caggagccgt tcctataaat ccatctaagg cagtattttc
     1801 ataaggaaat cttccttgcc aagtattggc atgataatat ccaccttcta aaagagtatc
     1861 tccccaagga tattcagatt gtcttcctcc tctagcagca tattcccact gtgcttcagt
     1921 cggtaaagac attccgctcc aattacagaa agccaatgca tcttctaaag caacatgaac
     1981 tactggatgg tcttctaaac ccactagatt actattctca ccgtagggat gtttccagca
     2041 agcgttcgga acttgtaacc accacggagc cccagcagga tgaggatatc cctccctttc
     2101 tgcctctgga acgaacaata taaaaacaaa agaccactct tgtcgctcag ctagtgtttt
     2161 atagcctgtt tctttcacaa attgagcaaa ttcttgattc gtaactggag tatccgcaat
     2221 ggaaaaatcc tttacactaa cagcgacttg aggaccttca aaatcatcta aaaagccctc
     2281 ttcagaattt gttcccatca taaatgtccc acccttgatt tttttcattt tcatgattga
     2341 ctatcccccg ttttcgttag agttggcgtt attttagaaa gttcaacagg aactaactct
     2401 tcatccacaa caaaaccttc ttctctatat ctaagaaagt ctactaattt tttcttcatc
     2461 tgacgaacaa taggttggta tttttcactt ggatacaaat cattcatttc atgcggatct
     2521 tttttcatat caaataactg ataatgattt aaaacaggaa accagataaa tttccactgg
     2581 tctgtcaaga tatattgact agaatctttt cctaaagcat gttcaccatg aaattctgtt
     2641 cgccacccct cgtactgtcc aaataataaa ttcttaacac tccgtccatc tagttcatct
     2701 gtagtagttc ctcctgccaa atccacgagt gaaggaaaaa tatcttgaat tttcactaat
     2761 tgcttgattg ttcccctatt accagctatc aatccagccg gatcgtaaat aaacgaagga
     2821 atgtggatac ttccctgata aggatatccc tttctaaaca gataatgttc accaagctga
     2881 tctccatgat ccgaaacaaa ccagataatc gtatctttat catgacgaaa ttcttttagg
     2941 gcagtcaaaa agcgactaat ttgatggtct atatgagtaa tcaatccaaa ataagcagca
     3001 accattcttc tttgatcatc ttcctttaat ttccctctta aagcataaat agacggaatt
     3061 tgcttttcta atacttccca attcccgata tgcaaatcga gaaattgagg caagcgttcc
     3121 atatacatat caaaatagta ttttggggga tttaatggag catgtggttt ttcaaaagac
     3181 attttgagaa aaaaaggaac cgttggatct cttctttgta aaaatgaaat ggactcactc
     3241 acaacccagt ttgttggatg taacttctca tctttatccc aaggtctagc ttcccaagaa
     3301 ttacaatcca ttccgtcatc aatcaaatca acatcataac caactttacc ctttaaaaag
     3361 gctagatagt cactagcata atcaaattga cttccataag ccttatcata ctttctatca
     3421 acatgtagat acccatcgtg aagtaagaca tgatcaaacc caagtctctg tctggaagga
     3481 aaaacatgca tcttaccaat acactctgtt tgataaccca tatctttaaa gacttttgga
     3541 agagtattag taaaattcca aggcacctca tcttgataac caactcgtcc acttttatct
     3601 tggtctaagc cagtcaataa agctgcccta gcaggaacgc aagaaggaac tggagaatag
     3661 gcattttcaa aattataacc tacactagcc atcatatcta atgttggagt gctaactagc
     3721 ttatccttac tattcaagga taaggcgtct gctctcattt gatctacaac aatcaaaata
     3781 atatttggct gttttgtctg aaccataaaa tctcctttct aatatggcaa aagaggcaca
     3841 agaagatatc tacctttact gcaccccttt ctatatcaat ctctctatat aaaacaataa
     3901 cattcttgtt atgttttata gaaaaatgaa ctaaaatatg actaaatcga ttaggaaatt
     3961 caaatcattt tctagtactg ttttagaagt tacagtgtac tattccaact tcaataaact
     4021 ataaaccttt gtctaataac aattttagtg gagataagaa atcctacacc taactcatct
     4081 tacacgtaat ctatttctat tttatcacaa aaaacgcaag taagaccatt aactcaattc
     4141 agttttatat gccattttca caaatgggaa ataagtcaag acactaataa tcaaacaaac
     4201 aactgataag atgatggcac gccaatcaaa tgctgtagag aagaaaccat ataaaattgg
     4261 aggcattacc caagtaacgt tttgtgtaac aggtgaaaca agaccccagc ttgttgccca
     4321 gtaagctacc gttgccatga aaaccgggct aagtacaaat ggtataaata gcaaaggatt
     4381 caagacaact ggtaaaccat aattgaccgg ctcaccaata ttaaacagaa ctggtgctag
     4441 accaagttta gcaacttttc gataatgact gtttcttgaa aaaattaaaa tagcaagtac
     4501 taatcctaat cctccaaacc agacaaacgc cccaaaagac ccacttgtcc atatataagg
     4561 aatcggttca cctttttgga aagcatccag attcgctaac atagcaactc caaacagccc
     4621 ttccatgatg ggagccaata catttcctcc atggagacca aaaaaccaga ataacttatt
     4681 caaaaagatc atcagaataa ctgcaaagaa actttgagac aaacctagta atggcgtttg
     4741 taacaccttg taaacccaat caatcaataa gtcattgcta agtaaatgga aaacataagt
     4801 caagatggct actatataca tcgccataaa tcctggaatg atagaagtga acggcttagc
     4861 aatcgcaggg ggaactgaat ctggtaactt gattacccag ttctttttca ttactttaca
     4921 gaaaataata gaggctaaaa atccaatcat catggctgta aagtagcctc tggcattaat
     4981 atggtttcct ggaatcacat tcccaatagt taccatcaga tttttaccat caaatgctag
     5041 attatcaatt ccttgtaaga tttgatctaa tttcacatct cctacatttg ccaaagggaa
     5101 actctttgta actgtacttc caatcgaaat gacaaacgaa gcaagtgata ccaaaccagc
     5161 agaaactgta tcaaccttgt aaatcttagc gatattcact cccaagcaat agatgaacaa
     5221 caaggaaaca attggtatac ttcccttgaa taccaaatta ttgatgtcaa caagccactg
     5281 aaaggttttc gtaatacttt ctaggtgaaa ttgttgtggt aaatccacta gaaaagcatt
     5341 taataacaaa gcaatggaac ctgtcataat aacaggcata gtccccacaa atgaatcacg
     5401 taaagcaact aaaaacttct gattcccaac tttcattgca attggagaaa gtttttcttg
     5461 cattttatcc aacattgtat tcataccaaa ctcctaattt ttttcaaatc gttcgtatac
     5521 ttctaccact tctttcgcca agtcaacaaa agtcagactc gtcattaaat gatcttgcgc
     5581 gtgtaccatc aagaggctaa ttgatgtttt ttccccctgt gcctctcttg tcaacatttc
     5641 tgtttgagcc ttatgcgcct gtaataaggc agcattcgca tcagctaatc ttcgattggc
     5701 tttagagaaa tccccttttt ttgctgcctg aatagcctcc atagcattac ttttggcttc
     5761 cccaccatac ataattagcc ccattacaga ttctaaatta ctttcatcca taacattctc
     5821 ctcaatctta gtctaataaa ctttcagcta aatctaaaac tttagaacca ttcatcattc
     5881 cgtaatctgt catcggaata accgccacag gaatctgtct atcttttagt ttttcttgaa
     5941 agtcccctag taaatagcga acttgaggac ctaaaagcaa cacatttact tcttttgttg
     6001 ctacaatttc ttctgcttca ggagctggaa ctgcaaaaat ttctgcatcc aaccccttat
     6061 cttctgctgc cttttgcatc tttgtcacta gcagacttgt actcatacct gctgcacatg
     6121 ctaacataat tgttacttta gccatagctt actcctttta ttttattgtc aatgttactg
     6181 tttttcgatc aactttagac tcataatcta aatgtttggg acgactagta taatgcaaat
     6241 tataaactcc tggaggcatt tgtgaaatct tttctttttc agttttattg cgaattaata
     6301 aatctaccga agtattccct ccaaagttat taaacatagg ttcattttca atagaataat
     6361 ctaactcata tccactatta gttgtaattc cttttaataa atcaggaaca tctcctctgt
     6421 ttattactat atccttagcg ccatttattt tgggagattg actataatac tcttcagctt
     6481 gatgttgttg taaatcattt ctagagtaag aaacataatc aactacaaat tcagtccctg
     6541 ataaattatt gtccactacc ccaggccaac ttcctcccat cgctaaattt aactgaacat
     6601 actgaggtct attaaaaatc tttttcgcta tattatctga tccataatat acttttctaa
     6661 caatatgatt gtcaaccatc catacaatat aatctttata ccaatcaatt gaaaaagtat
     6721 gatacgaatc attaaagttt ccactcgata atttaaaggc tgtaccacgt cctgcaaatt
     6781 ttccatcatc attatccgac tgtccataat gaagcgtttg ataaactgtc ttattcccac
     6841 ttccatttga atttcgttca ccaactaatt ccattatatc tatctcacct gttgacggcc
     6901 atcctcgtcc ctgaacagga ttaatcttcc catcaagtgt aaaatccgat ccaagagtcc
     6961 agaaagcagg aaaaactcct tgacctttcg gtaattttgc acgcatctct aatttaccat
     7021 aaagaaattc tactttccca tgtgttctaa cacttcctga attatataca acctttctgt
     7081 gattgtttct tgggttaaaa tactgatctt ccttagagcg attcgtagct ctaagaacta
     7141 aatttcccga acgtagaaaa acattctcat cacttcttac ataatgttgc tgttcccaac
     7201 cacgaattga tcctaattca tatccccaat attttttatt taattgttcg ccatcaaaat
     7261 catctctcca aattttctta tatgaatttc ctttagacag ttgatacatc tctacattat
     7321 caataaaaat attagatttt tttgtagtat cgtcttctga ccatttcact aattgaatca
     7381 aaactttatc gtgtttaccg gaattgaaag aaaacatgta ttttttccat tcggttgtat
     7441 taactgtttg atcaacagct tctccaatgt tattttgaac aatctctcct tgcttattat
     7501 tatcaaacca tcttgcggta aaatatccct ctgctccact aatatctact ttggcaaaag
     7561 cagatactaa ataatcttca ttaggttcta tatcaataac ttgtccaata tatccatctg
     7621 ctctattatc attaattgca gtagaaggaa tcatacctga gtaatgtccg ttactataac
     7681 tctcatttga attaattatt cccctatttc tagagttcca aaacagctct ccttgctcaa
     7741 aatttgaatt ttttattaaa ttttcattag caaaaacttt cgcatgaata aatattaata
     7801 aaaataatat aaataaactt aaaattaatt tcttattctt cattttatca cctatttact
     7861 gacctagcca agcgagatac tgtatgtctc actcctctaa ttgccttgct caatgcataa
     7921 atattatcga ctgttgccag tcccccatat cctgcgtcac ctatatgttg aatatcaact
     7981 ccacaaattt tatttctaag tgcaatttcc ttgatagtat ctgtatcaga tgtttcttgg
     8041 ctagtaccaa tagcactcaa tacaagacca cccttaccat gaacgagatc aacgacttca
     8101 cgcaactctt ggtcatgaaa ggctggaacg gttccaactg ctggaacaag tatcacatcc
     8161 gcacctgctt ccaacaattg ctctgcgaca gaaagctctg ccacaggctc attcactcct
     8221 gccccgtgca tcttacctgc aataatcaaa ccagaaaagt tttccttagc agtttgaaca
     8281 gccttaatga tttctcgatt gctaactcct gttccaggat ttccagtcaa acagacaaag
     8341 tcaaaaccta actcctctat tcgcttcaat gtttcaacac tagcaacacg acccgcaaca
     8401 atttcctgtg tttcctctag catctttgca gatgggtcaa tcggttccaa atttacacca
     8461 attggacaag caacaagctg gtgtaagcga tgaataacat catcacctga actatccaaa
     8521 gcataaattt taggctcaaa aacatcaaca caattcaata aaatcatatc agctccaaaa
     8581 gctctagcaa tttcagaatt agttatatcc ccaacaaatg tctcacgagt aactacattt
     8641 tcagatagaa caactctacc ttcacttgct aaaatacttt gttttaattc ttcagcattc
     8701 atctgtaata tctcagatgg atttgcacta attaaacgtt tcattctcaa tcctccaata
     8761 acttaaaccg tttttcaata gctattacaa atggtaaata aagtaagaca tccaaaacaa
     8821 tcaatacaat ttgtaataat gaaccagaaa tacttcctgt agcaagtaaa ccacttataa
     8881 caggtggcat agtccaacct ggatctgtat aagttagcgg aaccaaacct gatgccattg
     8941 ctccccacgc tactagtaag ttaaacattg gagctaagat aaatggaact agtaacaaaa
     9001 tgttcaaaac aataggaacg ccaaacattg ctggttcatt tatattaaat aatccaggta
     9061 taacagttat aggtgccaaa gtttttagtt gtttactagc tttcttctta tgtgctaaat
     9121 atcctaacgc aagaactaaa cctatggttg caccaccgcc acctatgaat acaaaattat
     9181 ccataaatac gctagtaaaa atatttggaa gagtttctcc agtttgataa gctactttat
     9241 ttgcatctag attagctaac cataacggtt tcataactgc attaacaaca tttcctccgt
     9301 gtaatcctac aaaccaaaaa ctactattaa ggaaaattaa gataagaaga ccaataacat
     9361 tatttcctaa aagtccaagt ggacctccca aagctacttg agcaatttca tgaagattag
     9421 gtaaacttaa tttatctaaa gttgcataaa caatcaacca accaacaata gtcactgcac
     9481 caggaataat ggcactaaag cttttagata ctgctggtgg aacaccatcc ggcattttta
     9541 tctgtatatt atgatttata aaccattggt agatatagcc attaatcaat cctaaaatca
     9601 tagcaacaaa taaacctttt gatgccatga aagcggtagg cattcctgct ccagcctctc
     9661 ctgtaatgaa aggcgtaact gtaataaaag atgataatga cacaatacca gcaggaatac
     9721 catcaacttt gtattgtcta gccataaagt aagcgatacc aaaactagct acaagaccaa
     9781 gtaatccaaa actactatca acacccttcc ataaatagtc agcaacacca atatcaccca
     9841 gatatttttc ccatcctgga ataggaaaac ttgcaataac cattagtaat gaacctatca
     9901 acaataaagg tatagttaga gtaataccgt ctcgaataga aaccaacgct ttattattac
     9961 ccaatctagc cgctactgga agtaactttt cttgtaataa tttttccata acct
//
LOCUS       DAVHEW010000078         9985 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVHEW010000078 DAVHEW010000000
VERSION     DAVHEW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.078"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..453
                     /locus_tag="VVL53_001655"
     CDS             <1..453
                     /locus_tag="VVL53_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4183800.1"
                     /translation="KNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREALITREKSF
                     PTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLF
                     FIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            491..793
                     /locus_tag="VVL53_001656"
     CDS             491..793
                     /locus_tag="VVL53_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4183801.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIRLPAKQNMV"
     gene            801..1605
                     /locus_tag="VVL53_001657"
     CDS             join(801..1169,1171..1605)
                     /locus_tag="VVL53_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU4183802.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANDSGKKKFHAMKAQEIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLIAEDKAYNHA
                     LSKDRSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGTINHELGF"
     gene            1654..2118
                     /locus_tag="VVL53_001658"
     CDS             1654..2118
                     /locus_tag="VVL53_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU4183803.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2275..2700
                     /gene="lacA"
                     /locus_tag="VVL53_001659"
     CDS             2275..2700
                     /gene="lacA"
                     /locus_tag="VVL53_001659"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU4183804.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2731..3246
                     /gene="lacB"
                     /locus_tag="VVL53_001660"
     CDS             2731..3246
                     /gene="lacB"
                     /locus_tag="VVL53_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU4183805.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3257..4186
                     /locus_tag="VVL53_001661"
     CDS             3257..4186
                     /locus_tag="VVL53_001661"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU4183806.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLTKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4188..5168
                     /gene="lacD"
                     /locus_tag="VVL53_001662"
     CDS             4188..5168
                     /gene="lacD"
                     /locus_tag="VVL53_001662"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU4183807.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5402..6238
                     /locus_tag="VVL53_001663"
     CDS             5402..6238
                     /locus_tag="VVL53_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU4183808.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6274..6591
                     /locus_tag="VVL53_001664"
     CDS             6274..6591
                     /locus_tag="VVL53_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4183809.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6591..8282
                     /locus_tag="VVL53_001665"
     CDS             6591..8282
                     /locus_tag="VVL53_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU4183810.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8497..9903
                     /gene="lacG"
                     /locus_tag="VVL53_001666"
     CDS             8497..9903
                     /gene="lacG"
                     /locus_tag="VVL53_001666"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU4183811.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILLVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         3169 a         1805 c         2070 g         2941 t
ORIGIN      
        1 aaaaaccttg tgttttgctt agaagcggat aatcaagaac aactctttga tcaggtagca
       61 agcttgttgg aagaacgaga aattgtcact ccaacttatc gtgaagcctt gatcacgcgt
      121 gaaaagtcat ttccaactgg tttagatatg gaatttctag gaaaggactt gccaaatgta
      181 gcgattcctc acacagatat tgttcataat ctagctgaaa aagtggtggt tgttcgatta
      241 gaaaaaccag taacttttca caatatgata gctcctgata aggaagtaga agtatcccta
      301 ctcttcttta tcattaacaa ttcaagttca agtcaaacaa atattctggc tcagttgatg
      361 gactttttca caggaaatgg acatcttgaa gatttatcaa aaatttccga accagaaaaa
      421 ctttatgctt acattgctga agcaaccgct taatcttgtc tattaaaaaa taaaatcgga
      481 ggaaatctaa atgattaaaa ttcttgctgc ctgcggtgca ggtgttaact caagtcacca
      541 aattaaaagt gctctagaag aagaactttc aaaccgtggt tatgatgttc actgtgatgc
      601 agtcatggtg aaagatgtaa acgaagacct tatgaaaggt tacgatatct ttacaccaat
      661 cgctgcaaca gaccttggtt ttgaaccagg tatcccagtt atcgaagctg ggccaatctt
      721 attccgtata ccagcaatga gcgctccagt atttgacaat attagactcc ctgcgaaaca
      781 aaatatggta tagtagttct atgaatgatg aagcaagtaa acaactaact gatgcacgat
      841 ttaagcgtct tgttggtgtt cagcgtacca cttttgaaga gatgttagct gtattaaaaa
      901 cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc
      961 ttcttatggc cactcttcaa tatgtgcgag aatatcgaac ttatgaagaa attgcggctg
     1021 attttggtat ccacgaaagc aacttactcc gtcggagcca atgggttgaa gtaactcttg
     1081 ttcaaagtgg tgttacgatt tcaagaactc ctctcagttc tgaggacacg gtaatgattg
     1141 atgcgacgga agtacaaatc aatcgcccta aaaaaagaat tagcgaatga ttctggtaaa
     1201 aagaaatttc acgctatgaa ggctcaagag attgtcacaa gtcaagggag aattgtttct
     1261 ttggatatca ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag tcgcagaaat
     1321 atcggacaag ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat
     1381 cctcaagcac aaactccacg taaatccagc aaactcaagc cgctaatagc tgaagataaa
     1441 gcctataacc atgcgctatc taaggataga agcaaggttg agaacatctt tgccaaagta
     1501 aaaacgttta aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga
     1561 atgaatttga gtgctggtac tatcaatcat gaactaggat tctagttttg caggaagtct
     1621 aataaagaaa ctagcaaggt caggccttac tttattggtg gacatccagg atttaattgt
     1681 cttctacttg atgatgaagt ctatgaagat tattatctag agtttgagaa agaagagatt
     1741 tgctctgttc cacgtccttt cccagaaaca ggtatgttgg atttccaaga tagaagtcca
     1801 tggctagagg gacaaaaaga aatagatctt agttatgatc ttttcagcac agatgcagtg
     1861 actttggatg aattgcaatc tcgaacaatt gcccttcgtt ctcttaaaca tgataaggga
     1921 ttgaaagtgc attttgcaga atttccaaac ctcatcatct ggtcaacttt gaacaaggga
     1981 cctttcatta cctttgaacc atggtctggc ttgtcaacat tccttgaaga aggagatcat
     2041 ttagaagata agaagaatgt ttgtctctta gaagccaatc aggttgaaga attagggttt
     2101 gagatagaag ttttataaaa aagtaaaaaa taaataacaa aaacaaacac aaactgttga
     2161 cttcttcaaa caatagtagt atattatgtt tgtgaggact aattgactgt tttttaaaga
     2221 cagccaatac actgctggaa tccagcatag aaaaaataaa aaggagtata caatatgtct
     2281 attgttatcg gtgcagatgc tgcaggtttg agattgaaag aagttgtgaa agacttctta
     2341 gaaaaagaaa acttccacct tgtggatgtt acagctgaag gtcaagactt tgttgatgtg
     2401 actctcgctg ttgctgcaga agtaaacaaa gaagaacaaa accttggtat cgtgattgat
     2461 gcttatggag ctggtccatt tatggttgca actaagatca aaggaatggt tgctgcagaa
     2521 gtatctgacg aacgttcagc ttatatgact cgtggccaca acaactcacg tatgatcact
     2581 atgggtgcac aacttgttgg tgatgaattg gctaaaaaca tcgctaaagg atttgttaac
     2641 ggtaaatacg atggtggacg tcatcaaatc cgggtcgaca tgttgaacaa aatgggctaa
     2701 ttagattaca gtaagaaagg taagttaaaa atgagaattg caattggatg cgaccacatc
     2761 gtaacagatg aaaaaatggc ggtttcagaa tttttgaaat caaaaggata tgaagtcatt
     2821 gactttggta catatgacca tacacggact cactacccaa tctttggtaa aaaagtaggg
     2881 gaagctgtaa ctagcggcca agctgatctt ggggtatgta tctgtggtac tggtgttggt
     2941 atcaacaacg ctgtaaataa agttccaggt gttcgttctg ccttggttcg tgatatgaca
     3001 acagcccttt atgctaaaga acaattgaac gctaacgtta tcggctttgg tggtaagatt
     3061 actggtgaat tgcttatgtg tgatatcatc gaagctttca ttcatgctga atacaaacca
     3121 tctgaagaaa acaagaaatt gattgcgaaa attgaacacc ttgaaagtca caatgctcaa
     3181 caaacagacg caaacttctt tacagaattc cttgaaaaat gggatcgcgg agaataccac
     3241 gactaagagg tgacctatga ttttaacagt cacaatgaac ccatccattg atatttccta
     3301 tcccttggat gagttgaaga ttgatactgt caatcgtgtg gtggatgtaa ccaaaacggc
     3361 tggtggtaag ggactcaatg ttacccgagt actttcagaa tttggcgatt ctgttcttgc
     3421 tactggttta gtgggtggca aacttggtga gtttttggtt gaacatatcg ataatcaagt
     3481 aaagaaagat ttcttctcaa ttcagggaga aactcgtaac tgtatcgcta ttctccacgg
     3541 agacaaccaa acagaagttc ttgaaaaagg tcctgaagta ttggaacagg aaggtcaaga
     3601 ctttttggaa catttcaaaa aactcttgga gtcagttgaa gtagtggcca tctcaggtag
     3661 tctgccagct ggccttccag ttgattacta tgcgagcttg gtagaacttg ctaatcaagc
     3721 tggcaagcct gtagtcttgg actgctcagg tgcagcactt caggctgttc ttgaatcacc
     3781 ccataaacca acagtcatca aaccaaataa tgaagaattg tctcagcttc ttggaagaga
     3841 agtttctgag gatttggatg aattaaaaga agtacttcaa gaacctttgt ttgcagggat
     3901 tgaatggatt atcgtttcac ttggtgccaa cggtactttt gccaaacatg gtgacacttt
     3961 ctacaaggta gatattccta gaattcaggt ggtaaatcct gttggatctg gagactctac
     4021 tgtggcagga atttcttcag gacttcttca caaagaatcg gatgcagaat tactcaccaa
     4081 ggcaaatgtc cttggtatgc tcaatgctca agaaaaaatg actggtcatg tcaacatggc
     4141 caactatcaa gttctatatg atcaattaat agtaaaagag gtataaaatg gctttaacag
     4201 aacaaaaacg tgtacgctta gaaaaacttt ctgatgaaaa tggtatcatc tcagctcttg
     4261 catttgacca acgtggtgct ttgaaacgcc tcatggctca acaccaaaca gaagaaccaa
     4321 ctgtggccca aatggaagaa cttaaagtct tggtagcaga tgaattgact aaatacgctt
     4381 catctatgct tcttgaccct gagtatggac ttccagcaac taaagctctt gatgaaaaag
     4441 ctggtcttct ccttgcttat gaaaaaacag gttatgacac aacaagcaca aaacgcttgc
     4501 cagactgctt ggatgtctgg tctgcaaaac gtattaaaga agaaggtgca gatgcagtta
     4561 aattcttgct ttactatgat gtagatagct cagacgaact caatcaagaa aaacaagcct
     4621 acatcgaacg catcggttct gagtgtgtgg ctgaagatat cccattcttc cttgaaattc
     4681 ttgcttacga tgaaaaaatt gcggatgcag gttctgtaga atacgcgaaa gtaaaaccac
     4741 acaaagttat cggcgctatg aaagtctttt cagacccacg ctttaacatt gatgtcttga
     4801 aagttgaagt tcctgttaac attaaatatg ttgaaggctt cgctgaaggt gaagtagttt
     4861 atacacgtga agaagcagca gccttcttca aagcgcaaga tgaagcaacg aacttgccat
     4921 acatctactt gagtgctggt gtatcagcta aactcttcca agatactctt gtatttgctc
     4981 atgaatcagg tgcaaacttt aacggagttc tttgtggccg tgctacatgg gcagggtcag
     5041 ttgaagctta catcaaagat ggtgaagcag cagctcgcga atggcttcgc acaactggat
     5101 ttgaaaacat tgatgagctc aataaagttc ttcaaacaac agcgacttca tggaaagaac
     5161 gtgtgtaaga aagtcctcct agtttaggaa catgaatcta aaaaaattta aaaaaagttg
     5221 tatgtaaagg tttacaaaat aacttacttg tgctatactt aaatcacaag ttaatacaag
     5281 gtgagtgtta ctaagtaata ttaggcatga tcacaggtga attagaaatc agctgatttt
     5341 ctagttcatt tgtggtcatt ttttgtactt atataccttt aagatataaa aggaggttga
     5401 catgtatcga attctaaatc caatgaatca caatgtctcg cttgtcagaa atgataaggg
     5461 agaagaggtg attgtaattg gtaaggggat tgcattcgga aagaagaagg gggatttgat
     5521 tgctgaaaat caggttgaga aaatctttcg gatgaagacc gaagagtcca gagaaaactt
     5581 tatggctctt ctcaaagatg ttccgcttga ttttatcaca gtgacctatg aaatcattga
     5641 taagctatca aagaaatatc attatccgat tcaagagtat ctctatgtaa ccttgacaga
     5701 tcatatttac tgttcttatc aagctctaac tcaaggaagg tacaaggata gtaatctgcc
     5761 agatatttcc gctaagtatc ctgtcgcttt tcaaatcgca aatgaagctt ttgaaattta
     5821 ccgtcagaag ctagcagatc attttcctga ggacgaaatt attcggattg cttatcattt
     5881 cattaatgct gaaggtgaaa atgaagtgga acttgtggag tcgattgata agaggaaaga
     5941 aattctcagg aatgttgaag aagttttaac ggactatgca attcaacgaa ctaaaaagaa
     6001 taaccatttc tatgatcgct ttatgatcca tttgaattat ttcttggatt atttagacag
     6061 atctagagat gataaccaat cacttctgga tatggaagat catattaaac aatcctatcc
     6121 aaaagccttc gagattggtt ccaagatcta tgatgtgatt acgcaacata cgggtcttga
     6181 tttgtataaa agtgaacgag tttatctagt tctacatatc caacgtttat tgtcataaaa
     6241 atttatttaa aactatataa ggagaattct atcatgaata gagaagaagt aacattgtta
     6301 ggttttgaaa tcgtagccta tgctggcgat gctcgttcaa aactattgga agccttgaag
     6361 gctgctgaag ctggtgattt tgaaaaagcg gacgctctgg tagaggaagc tggtagctgt
     6421 attgcagagg ctcaccacgc gcaaacaagt ctattgacta aggaagcttc aggtgaggac
     6481 ttggcttata gtgtaaccat gatgcatggc caagaccact taatgacaac tatcttgtta
     6541 aaagatttga tgcatcattt aattgaactc tacaagagag gagttaaata atgaataaac
     6601 taattgcatt tatcgagaaa ggaaagcctt tctttgaaaa actatctcgt aatatctatc
     6661 ttcgtgctat tcgtgatggt ttcattgcag gtatgcctgt tattctcttc tcaagtatct
     6721 ttatcttgat tgcctttgta ccaaactcat ggggctttaa atggtctgat gaagttgtag
     6781 cctttctgat gaaaccttat agctattcta tgggtattct ggctctcttg gtagctggta
     6841 caacagctaa gtcattgact gactcagtaa accgtagcat ggaaaaaacc aatcaaatca
     6901 actatatgtc aacattgttg gcagcaattg ttggtttgtt gatgttggca gctgatccta
     6961 tcgaaagtgg tctagctact ggattcttgg ggacaaaagg tttgctttca gccttccttg
     7021 ctgccttcgt tactgtagcc atctataagg tttgtgttaa gaacaacgtc actattcgta
     7081 tgcctgacga agttccacca aatatctcac aagtctttaa agatgtgatt ccattcactc
     7141 tctcagttgt ttcactttat gctcttgatt tactagctcg tcattttgtt ggtgcaagcg
     7201 tagctgaatc aatcggtaaa ttctttgcac cactcttctc agcagcagac ggataccttg
     7261 gtattaccat tatctttggt gcctttgcct tcttctggtt tgtgggtatt catggtccat
     7321 ctattgttga acctgctatc gcagcaatta cctatgcaaa tgctgaagtc aacttgaacc
     7381 ttctccaaca aggtatgcac gctgacaaga ttcttacttc tggtacacaa atgtttatcg
     7441 ttaccatggg tggtactggt gcgacactgg ttgttccatt catgttcatg tggttaacaa
     7501 aatcaaaacg taaccgtgca atcggacgtg cttcagtagt tcctaccttc ttcggtgtaa
     7561 atgagccaat cttgtttggt gcacctcttg ttttgaatcc aatcttcttc attccattta
     7621 tctttgctcc aattgcaaac gtatggatct ttaaattctt tattgaaact cttggaatga
     7681 actcattcac tgctaatcta ccatggacaa ccccagctcc actaggtcta gttcttggta
     7741 ctaacttcca agttctatca ttcattcttg ctgcccttct aatcgtggtt gacgttgtca
     7801 tttactatcc attccttaag gtctatgatg aacaaattct tgaagaagaa cgttcaggta
     7861 agtctaatga tgaattgaaa gaaaaagttg ctgcaaactt caacactgca aaagcggatg
     7921 ctattcttga aaaagcaggt gtagaagcag cacaaaatac aatcactaaa gaaacaaatg
     7981 tcctcgttct ttgtgcaggt ggaggaacaa gtggtctcct tgcaaatgct ctaaacaagg
     8041 cagcagcaga atacaatgtc cctgtgaaag cagcagcagg tggctatggt gctcaccgtg
     8101 aaatgttgcc agagtttgat ttggttatcc ttgctcctca agttgcttca aactttgaag
     8161 atatgaaggc agaaacagat aagctcggta ttaaacttgc taagacagaa ggcgctcaat
     8221 acatcaaatt aactcgtgat ggaaaaggtg ccctagcatt cgtacaagag caattcgatt
     8281 aaggctagga actgtgaaac agtctcccat cgttacggaa atcgctatgg cgaatttcct
     8341 aatcgcctta ttaattcgtc ggtaaaaaga tatcgttttt acctcctcat gtcacaattc
     8401 ggtgacttgg tacaagaagt gagatggaga aggatggctc actgactcct ctcctctcac
     8461 ttttacttta tttaaatcaa gaaataggtg aaaaaaatga caaaaacact tccaaaagac
     8521 tttatttttg gtggcgcaac agctgcctat caagcagaag gtgctacaca tactgatggt
     8581 aaaggaccag tagcgtggga taaatatctt gaagataact actggtacac tgcagaacca
     8641 gccagtgatt tttacaatcg atatccagtt gacctcaagc tagcagaaga gtatggtgtc
     8701 aatggcattc gaatttctat tgcctggtca cgtattttcc cgactggtta tggtcaagta
     8761 aatgctaaag gtgttgagtt ttatcataat ttatttgcag agtgtcacaa acgtcatgtt
     8821 gagccctttg taactcttca tcactttgac acgccagaag ctctccactc aaatggagac
     8881 ttcttaaacc gtgaaaatat cgaacatttt gtagactacg ctgccttctg ttttgaagaa
     8941 tttccagaag taaactattg gacaaccttt aatgaaattg gaccaatcgg tgatggtcaa
     9001 tatttggttg ggaaattccc tccaggtatc cagtacgacc ttgccaaagt ctttcaatca
     9061 caccacaata tgatggtgtc tcatgcacgc gcggtaaaat tgtacaaaga gaaaggctat
     9121 aaaggggaaa ttggtgttgt tcacgccctg cctactaaat atcctctaga tcctgaaaat
     9181 ccagcagatg ttcgtgcagc agagttggaa gatatcatcc acaataaatt catcttagac
     9241 gcaacttatc taggtcgcta ttcagctgaa accatggaag gtgtcaacca tatcttatta
     9301 gtcaatggtg gtagtttgga tcttcgtgaa gaagatttta cagcattaga agctgcaaaa
     9361 gacttgaatg atttcctagg aatcaactac tatatgagtg actggatgga agcctttgat
     9421 ggagaaactg aaattatcca taatggtaaa ggtgaaaaag gaagctctaa gtatcaaatc
     9481 aaaggtgttg gtcgtcgtgt agctcctgac tatgtaccac gcacggattg ggattggatt
     9541 atctaccctc aaggtttgta tgaccaaatc atgcgtgtga agaaagatta tcctaactac
     9601 aagaagattt acatcactga aaatggtctc ggctataaag atgagttcgt tgataacact
     9661 gtttacgatg atggtcgtat tgattacgtg aagcaacact tggaggtttt atctgatgcg
     9721 attgcagatg gagctaatgt aaaaggttac ttcatttggt cattaatgga tgtcttctca
     9781 tggtcaaacg gttatgagaa acgttatggt ctcttctacg tagattttga aactcaagaa
     9841 cgttatccta agaaatcagc tcactggtac aagaaagtag cggaaactca gattatagac
     9901 tagtagaatt agtcattaga tatagaattt tagtgagtca aaaagatgtt caaagatttt
     9961 atccaatcta tttatgaaaa aaagt
//
LOCUS       DAVHEW010000079         9846 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVHEW010000079 DAVHEW010000000
VERSION     DAVHEW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.079"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            34..6048
                     /locus_tag="VVL53_001667"
     CDS             34..6048
                     /locus_tag="VVL53_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU4183812.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSKNTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVG
                     NKVGHNQHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVH
                     NMFDVVYMLEYLEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEE
                     VERLRSFNDLIDNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRR
                     IAYELLAAKGFKDGMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQY
                     HTWSEFKKAMYQERQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKD
                     AEHNYYHWNNYNPDIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            6100..6276
                     /locus_tag="VVL53_001668"
     CDS             6100..6276
                     /locus_tag="VVL53_001668"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4183813.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            6495..7346
                     /gene="ylqF"
                     /locus_tag="VVL53_001669"
     CDS             6495..7346
                     /gene="ylqF"
                     /locus_tag="VVL53_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU4183814.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            7333..8112
                     /locus_tag="VVL53_001670"
     CDS             7333..8112
                     /locus_tag="VVL53_001670"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU4183815.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILSKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELLKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8128..9678
                     /locus_tag="VVL53_001671"
     CDS             8128..9678
                     /locus_tag="VVL53_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU4183816.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3386 a         1806 c         1993 g         2661 t
ORIGIN      
        1 taacatatat aaattttatt tcaaggagga ataatggaaa agtattttgg tgaaaaacaa
       61 gagcgttttt catttagaaa attatcagta ggacttgtat ctgcaacgat ttcaagttta
      121 ttttttatgt ctgtattagc tagttcatct gtggatgctc aagaaactgc gggagttcac
      181 tataaatatg tggcagattc agagctatca tcagaagaaa agaagcagct tgtctatgat
      241 attccgacat acgtggagaa tgatgatgaa acttattatc ttgtttataa gttaaattct
      301 caaaatcaac tggcggaatt accaaatact ggaagcaaga atgagaggca agccctagtt
      361 gctggtgcta gcttagctgc tctgggaatt ttaatttttg ctgtttccaa gaaaaaggtt
      421 aagaataaaa cggtattaca tttagtattg gttgcaggga taggaaatgg tgtcttagtt
      481 tcagtccatg ctttagaaaa tcatcttttg ctaaattaca atacggacta tgaattgacc
      541 tctggagaaa aattacctct tcctaaagag atttcaggtt acacttatat tggatatatc
      601 aaagagggaa aaacgacttc tgagtctgaa gtaagtaatc aaaagagttc agttgccact
      661 cctacaaaac aacaaaaggt ggattataat gttacaccga attttgtaga ccatccatca
      721 acagtacaag ctattcagga acaaacacct gtttcttcaa ctaagccgac agaagttcaa
      781 gtagttgaaa aacctttctc tactgaatta atcaatccaa gaaaagaaga gaaacaatct
      841 tcagattctc aagaacaatt agccgaacat aagaatctag aaacgaagaa agaggagaag
      901 atttctccaa aagaaaagac tggggtaaat acattaaatc cacaggatga agttttatca
      961 ggtcaattga acaaacctga actcttatat cgtgaggaaa ctatagagac aaaaatagat
     1021 tttcaagaag aaattcaaga aaatcctgat ttagctgaag gaactgtaag agtaaaacaa
     1081 gaaggtaaat taggtaagaa agttgaaatc gtcagaatat tctctgtaaa caaggaagaa
     1141 gtttcgcgag aaattgtttc aacttcaacg actgcgccta gtccaagaat agtcgaaaaa
     1201 ggtactaaaa aaactcaagt tataaaggaa caacctgaga ctggtgtaga acataaggac
     1261 gtacagtctg gagctattgt tgaacccgca attcagcctg agttgcccga agctgtagta
     1321 agtgacaaag gcgaaccaga agttcaacct acattacccg aagcagttgt gaccgacaaa
     1381 ggtgagactg aggttcaacc agagtcgcca gatactgtgg taagtgataa aggtgaacca
     1441 gagcaggtag caccgcttcc agaatataag ggtaatattg agcaagtaaa acctgaaact
     1501 ccggttgaga agaccaaaga acaaggtcca gaaaaaactg aagaagttcc agtaaaacca
     1561 acagaagaaa caccagtaaa tccaaatgaa ggtactacag aaggaacctc aattcaagaa
     1621 gcagaaaatc cagttcaacc tgcagaagaa tcaacaacga attcagagaa agtatcacca
     1681 gatacatcta gcaaaaatac tggggaagtg tccagtaatc ctagtgattc gacaacctca
     1741 gttggagaat caaataaacc agaacataat gactctaaaa atgaaaattc agaaaaaact
     1801 gtagaagaag ttccagtaaa tccaaatgaa ggcacagtag aaggtacctc aaatcaagaa
     1861 acagaaaaac cagttcaacc tgcagaagaa acacaaacaa actctgggaa aatagctaac
     1921 gaaaatactg gagaagtatc caataaacct agtgattcaa aaccaccagt tgaagaatca
     1981 aatcaaccag aaaaaaacgg aactgcaaca aaaccagaaa attcaggtaa tacaacatca
     2041 gagaatggac aaacagaacc agaaccatca aacggaaatt caactgagga tgtttcaacc
     2101 gaatcaaaca catccaattc aaatggaaac gaagaaatta aacaagaaaa tgaactagac
     2161 cctgataaaa aggtagaaga accagagaaa acacttgaat taagaaatgt ttccgaccta
     2221 gagttataca gtttgtcaaa tggtacttat aaacaacaca tttcgttaga gcaagttcca
     2281 agcaatccaa atagctactt tgttaaagtg aaatcttctt cattcaaaga tgtataccta
     2341 ccagtagcat caatatcaga ggaaagaaaa aatgataaaa tcctttataa aatcacagca
     2401 aaagtagaga agcttcagca ggagatagaa agcagatata aagataattt taccttctat
     2461 ctagctaaga agggaacaga agaaacaaca aactttactt cctttagtaa tctggtcaaa
     2521 gctataaacc aaaatccctc tggaacctat catttagcgg ccagcctgaa tgctaacgaa
     2581 gtggagcttg gtcctgatga aagatcctat atcaaggaca cctttactgg tcgtttaatc
     2641 ggtgaaaaag atggcaagaa ttatgctatc tataatttga aaaaacctct gtttgaaaac
     2701 ttgagtggtg ctacagtaga aaaactgagt ctaaaaaatg ttgctatttc agggaaagat
     2761 gatatcggtt cactggcaaa tgaagctcag aataacacaa aaattaagca agttcacgtc
     2821 gatggtgttc tggctggtga acgtggtatc ggtggtttgc tggctaaggc tgagcaatca
     2881 agcatcacag agagcagttt caagggaaga attatcaaca cttatgaaac gactgctgcc
     2941 tacaatatcg gtggtatggt cggtcatttg acaggtgaca aggctttact tactaagtca
     3001 aaagcgacag tagccatttc atctaacaca aatacttcag atcagactgt gggtggactt
     3061 gcaggcctag tagaccgaga tgcacagatc caagatagct atgctgaagg tgatatcaac
     3121 aatgtcaagc actttggtag agtcgctgga gtggcaggca atttgtggga tcgaacttct
     3181 ggtgatgtta ggcatgctgg aagtttgacc aatgttctca gcgatgttaa tgtaaccaac
     3241 ggaaatgcca tcactggtta ccactataac gaaatgaagg taaaggacac attcagcagc
     3301 aaggccaaca gagtctacaa tgtcaccttg gtcaaggatg aggtcgtcag caaggaatcc
     3361 tttgaagaaa gaggaacaat gctagatgct tctcaaattg caagcaaaaa agcagaaatc
     3421 aatcctctca ttctaccaac agtggagcca ctttcaacaa gtggcaaaaa agacagtgat
     3481 ttttctaagg tggcctatta tcaagctaag cgcaacttga cttataaaaa cattgaaaaa
     3541 ttgctacctt tctacaacaa ggcaaccatc gtcaaatacg gaaacctggt caatgagaac
     3601 agtcttttat atcaaaaaga actcttgtca gcagtcatga tgaaggacaa ccaagtcatc
     3661 acagacattg tttctaacaa acagactgca aacaaactct tgcttcacta caaggatgat
     3721 ttatctgaga agctggatct caaataccag aatgatttcg ccaaattagc agaatatagt
     3781 ctgggcaata ctggacttct ctatacgcca aaccaattcc tgtatgacca aacctctatc
     3841 atcaagcaag tcttacctga cttacaaaag gttgactatc attcagaagc catcagaaag
     3901 acgctgggta tttctccaaa cgtcaagcaa actgagctct atctagaaga ccagttcgcc
     3961 aaaacaaaac aacaactgga agacagtttg aaaaaactct tgtcagcgga tgctggactg
     4021 gctagtgcta accccgtcac tgaaggttat cttgtagata aaatcaaacg caacaaggaa
     4081 gccttgctac ttggcttgac ctatctggaa cggtggtata actttagcta tggtcaggtg
     4141 aatgtcaaag acctagttct gtaccatttg gacttctttg gtaaggggaa tgcttcacca
     4201 ttagatactc tgattgagtt gggtaaatct ggctttaaca atcttctagc taagaataat
     4261 gtcgatactt atggtatcag tcttgccagt caacatggaa cgacagattt gtttagcacg
     4321 ctggaacatt accgaaaagt ctttttacca aatacaagca ataatgactg gtttaaatca
     4381 gagactaagg cttacattgt cgaagaaaaa tccactatcg aagaggtgaa aacgaagcaa
     4441 gggttagctg gcaccaagta ttctatcggt gtttatgatc gtatcacgag tgccacatgg
     4501 aaataccgca atatggtctt gcctctcctg accttgccag agagatccgt atttgtcatc
     4561 tcgaccatgt ctagtctagg atttggagct tatgatcgct accgcagtag tgaccataaa
     4621 gcgggcaagg ctctcaatga ttttgttgaa gaaaatgcgc gtgaaacagc caaacgtcag
     4681 cgagatcact acgattattg gtatcgtatt ttagacgaca atgcacgtga aaaactttat
     4741 agaaatattt tgctttacga tgcttataaa tttggcgatg ataataccgt agggaaagct
     4801 acagaagtgg cagattttga taatccaaat cctgcaatgc aacatttctt tggacctgtt
     4861 ggaaataaag ttgggcataa tcaacacggt gcttatgcta caggtgatgc agtttattat
     4921 atgggttatc gaatgttgga taaggatgga gctattactt atacgcatga gatgacacat
     4981 gactcagatc aggacattta tcttggagga tatggtcgaa gaagtggctt gggaccagag
     5041 ttctttgcta aaggattatt acaagcacca gaccatccag atgatgcgac cattaccatc
     5101 aactccatct tgaaacattc aaaatctgat agtacagaaa gtcgacgatt acaagtactt
     5161 gatccaacta caagatttaa taatgcagat gatttgaagc aatatgtcca caacatgttt
     5221 gacgttgttt atatgttgga atatctcgaa ggaaattcaa ttcttaaatt ggatacgaat
     5281 caaaaacaac aacttcttag aaaagttaca aatgagtacc atcctgatcc tgatggaaat
     5341 aaggtctatg caacaaatgt tgtcagaaat ctaacagtag aagaagttga aagactacgt
     5401 tcattcaatg atttgattga taataatatt ctttcgtcta gggaatatgc ctcaggtaaa
     5461 tacgaaagaa atggctactt cactattaag ttatttgcac cgatttatgc tgcattaagt
     5521 aatgatatag gaacaccagg tgacctgatg ggacgtcgta tagcctatga actactagct
     5581 gctaaaggct ttaaagatgg tatggtacca tatatctcaa accaatacga agaagaagcc
     5641 aaacaaaagg gcaagacaat caatctctac ggtaaaacaa gaggtttggt tacagatgac
     5701 ttggttttgg aaaaggtatt taataaccaa tatcatactt ggagtgagtt taagaaagct
     5761 atgtatcaag aacgacaaga tcagtttgat agattgaaca aagttacttt taatgataca
     5821 acacagcctt ggcaaacatt tgccaagaaa actacaagca gtgtagatga attacagaaa
     5881 ttaatggacg ttgctgttcg taaggatgca gaacacaatt actaccattg gaataactac
     5941 aatccagaca tagatagtga agtccacaag ctcaagagag caatctttaa agcctatctt
     6001 gaccaaacaa atgattttag aagttcaatt tttgagaata aaaaatagtg tctactatta
     6061 ggaaataaag tttaaaaagg tgatgaagaa caaaccaaga ttcaagcagg aattcctact
     6121 gataatgaag taagttatga tcttatttat cagcaggaaa ctcttcctgc aacaggttca
     6181 tcaacttctg agcttacagc tttaggccta ttagctgttg gtagtttagt tcttttggtt
     6241 cataatatga cgggaacagt tttttgctcc ctctgaaaag tcatcatttg atggcttttt
     6301 tctatatagg gtaaaagata gggtaaaagg ctatcatcgg acaaaataaa gaaggcatga
     6361 tataatataa agtagatttc tatgtcataa aacaagaact gtttggacat cattcatttg
     6421 aaaactctct atgttcaaac aatagtaaaa taaaataggg gatctaaatc cttgctatga
     6481 aaggaaaaaa ctcaatggct actattcaat ggtttcctgg tcacatgtct aaagctcgtc
     6541 gacaggtgca ggagaattta aaatttgttg attttgtgac gattttagta gatgcacgct
     6601 tgcctctatc tagtcaaaat cctatgttga ccaagattgt tggtgataaa ccaaaactct
     6661 tgattttaaa caaggccgac ttggctgatc cagcaatgac caaggaatgg cgtcagtatt
     6721 ttgaatcaca aggaatccag acgctagcta tcaactccaa agagcaagtg actgtaaaag
     6781 ttgtaacaga tgcggccaag aagctcatgg ctgataagat tgctcgccag aaagaacgtg
     6841 ggattcagat tgaaaccttg cgtactatga ttatcgggat tccaaacgct ggtaaatcaa
     6901 ctctgatgaa ccgtttggct ggtaaaaaga ttgctgttgt tggaaacaag ccaggggtca
     6961 caaaaggtca acaatggctt aaaaccaata aagacctgga aatcttggat acaccgggga
     7021 ttctctggcc taagtttgag gatgaaactg ttgcacttaa gttggcattg actggagcta
     7081 tcaaagacca gttgcttcct atggatgagg ttaccatttt tggtatcaat tatttcaaag
     7141 aacattatcc agaaaagctg gctgaacgct tcaaacaaat gaaaattgaa gaagaagcgc
     7201 ctgtgattat tatggatatg acccgcgccc tcggtttccg tgatgactat gaccgttttt
     7261 acagtctctt cgtgaaggaa gtccgtgatg gcaaactcgg taactatacc ttagatacat
     7321 tggaagacct cgatggcaac gattaaagaa atcaaagaat tccttgtgac agtcaaggag
     7381 ttagaaagcc ctattttttt agagcttgaa aaggataatc gctcaggagt tcaaaaggaa
     7441 atcagcaagc gtaaaagagc cattcaagct gaattagatg aaaatttgcg cttggaatcc
     7501 atgctttctt atgaaaaaga actttataag caaggattga ccttaattgc aggtattgat
     7561 gaggttggtc gtggtcctct tgctggtcct gtagtcgctg cggccgttat tttatctaaa
     7621 aattgtaaga ttaaaggtct caacgacagc aagaaaattc ctaaaaagaa acatctggag
     7681 attttccaag ccgttcaaga ccaagccttg tcgattggaa ttggtatcat agataatcag
     7741 gtcatcgacc aagtcaacat ctatgaagca accaaactag ccatgcaaga agcaatctcc
     7801 cagctcagcc ctcaaccaga gcaccttttg attgatgcca tgaaactgga cttgcccatt
     7861 tcacaaacct ccattatcaa aggagatgcc aactccctct ctatcgcagc agcatctata
     7921 gtagccaagg taacacgtga tgaattgctg aaagaatacg atcagcagtt ccctggctat
     7981 gatttcgcta ctaatgcagg atatggcaca gctaaacatc tggaaggcct cacaaaacta
     8041 ggagttaccc caattcaccg aaccagcttt gaacccgtta aatcactggt tttaggtaaa
     8101 aaagaaagtt aattgaaagg aaataacatg gaggaacagt cggaaatagt ccgttctaag
     8161 aaagaattcg cctttgcatc cagcactata ctatcccaag ttggtcgagg aatcattgtc
     8221 ggcctcatcg ttggaattat cgtcggatcc tttcgtttct taattgaaaa gggcttccac
     8281 ctgatacaag gagtttatca agatcaaggg tacttagtgc gcaatctttt tgtactggtt
     8341 ttgttttata tactcatctg ttggctcagt gccaaactaa cacggtcaga aaaagatatt
     8401 aaaggctcag gaattcctca agtcgaagcc gaactgaaag gcctcatgtc cctcaactgg
     8461 tggggcattc tttggaaaaa atatgtgcta ggtattcttg ctattgccag tggactcatg
     8521 ctgggtcgag agggacccag cattcaactt ggagcagttg gtggtaaagg aattgccaag
     8581 tggctcaaat ccagtccagt agaggaacgt tccttgattg ccagtggagc tgcagcaggt
     8641 ttagccgcag cctttaatgc tcctattgca gcacttctct ttgttgtaga agaagtctat
     8701 caccattttt cgcgcttttt ctgggtctca actctagcag ccagcatcgt agcaaacttt
     8761 gtgtctctac tcatgttcgg tttgacacca gtattggata tgccagataa cattcctccc
     8821 atgaccctag atcagtattg gatatatctc gtcatgggaa ttttccttgg attttcaggt
     8881 tttctctatg agaaagctgt attaaacgtt ggaagagttt atgacttgat tggtcaaaaa
     8941 atccatttgg atagggctta ttatcccatc ttggctttta tccttatcat accagtcgga
     9001 atcttcttac ctcaaatcat tggtggcgga aatcagcttg tcctttcttt aactgaacaa
     9061 aattttagtt tccaagtttt attagcttac tttttaatcc gctttatttg gagtatgatt
     9121 agctatggaa gtggactgcc aggaggaatt ttcctcccca ttttagctct tggttctttg
     9181 cttggtgcct tagttggtgt tatctgtgtc aatcttggac ttgtcagtca agagcaattc
     9241 cctatatttg tcattctagg aatgagtggc tattttggag ccatatcaaa agctccctta
     9301 accgctatga tcctcgtaac tgagatggta ggagatattc gcaaccttat gccacttggt
     9361 cttgtcactc ttgtttctta tattatcatg gatttgctca aaggtacgcc agtctatgaa
     9421 gccatgctgg aaaaaatgct tccagaagaa gtatctagcg aaggagaagt tacacttatc
     9481 gaaataccag tttctgataa aattgctggg aaacaagttc atgaactcaa cttaccacac
     9541 aacgtcctca tcacaactca agtccataat ggcaagagcc aaacagttaa cggctcaacc
     9601 agaatgtatc tgggtgatat gattcacctg gttattccaa aaagtgaaat tggaaaagtc
     9661 aaagatttgt tgttgtagta tgagtattta cataatttat gttatgtaaa tgatcagttt
     9721 gatttattta gaaaaccgat tctcaggaat gagatcggtt attttttact gatgaggaat
     9781 tttacatata aataattgaa ctttattaaa aataagacta taattaagtt agaaatgata
     9841 aagtat
//
LOCUS       DAVHEW010000080         9814 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVHEW010000080 DAVHEW010000000
VERSION     DAVHEW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.080"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(184..1854)
                     /locus_tag="VVL53_001672"
     CDS             complement(184..1854)
                     /locus_tag="VVL53_001672"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU4183817.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRTLRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2084..2773
                     /gene="coaB"
                     /locus_tag="VVL53_001673"
     CDS             2084..2773
                     /gene="coaB"
                     /locus_tag="VVL53_001673"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU4183818.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGYEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2785..3336
                     /gene="coaC"
                     /locus_tag="VVL53_001674"
     CDS             2785..3336
                     /gene="coaC"
                     /locus_tag="VVL53_001674"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU4183819.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3320..3883
                     /locus_tag="VVL53_001675"
     CDS             3320..3883
                     /locus_tag="VVL53_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4183820.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            4165..4692
                     /locus_tag="VVL53_001676"
     CDS             4165..4692
                     /locus_tag="VVL53_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4183821.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWIFNFVLAVVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            4704..5219
                     /locus_tag="VVL53_001677"
     CDS             4704..5219
                     /locus_tag="VVL53_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU4183822.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQRLAEHFHVTRQVIVQDIAILR
                     ADGSPILSTNRGYIYKDANANTYHHKLFKVKHEVEEIGRELLAIVDNGGRVQNILIDH
                     PVYGEIETLLKLTCRRDVQHFLEQVERSDFKPISELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5216..5683)
                     /locus_tag="VVL53_001678"
     CDS             complement(5216..5683)
                     /locus_tag="VVL53_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU4183823.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMDAPDKSEFFYRHRTEDDWEKKIF"
     gene            complement(5752..6240)
                     /locus_tag="VVL53_001679"
     CDS             complement(5752..6240)
                     /locus_tag="VVL53_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183824.1"
                     /translation="MTVIIKSMETPEEIEGKSFVHWQTWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAMDGVKIVGFISYGNCRDETIQAGEIIALYVLKDYYGKGIAQK
                     LVKAALTDLNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(6206..6769)
                     /locus_tag="VVL53_001680"
     CDS             complement(6206..6769)
                     /locus_tag="VVL53_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183825.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(6779..8767)
                     /gene="uvrB"
                     /locus_tag="VVL53_001681"
     CDS             complement(6779..8767)
                     /gene="uvrB"
                     /locus_tag="VVL53_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU4183826.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(8844..9797)
                     /locus_tag="VVL53_001682"
     CDS             complement(8844..9797)
                     /locus_tag="VVL53_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4183827.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2919 a         2184 c         1762 g         2949 t
ORIGIN      
        1 cactgttttg aggttgtgga tagaactgac agagtcagta tcatatacct acggcaaggt
       61 gaagctgacg tagtttgaag agattttcga agagtataaa tcttattgat gaactgcttg
      121 cagtctgaga aaaaatgagc ttggatatta tttccaaact cacttaaagt caatttcaat
      181 ccactagaac aagcctagta cagttccatc gctttcaaca tccatgttga gagctgctgg
      241 acgttttgga agacctggca tggtcataac atcaccagtt aaggcaacga tgaagcctgc
      301 acctaatttt ggtaccaatt cacgaatggt aatttcaaag ttttctggtg ctccaagcgc
      361 atttggattg tctgagaaac tgtattgagt tttagccata cagattggca atttgtccca
      421 accgttttga acgatttgag caatttgtgt ttgagctttc ttctcaaagt tcactttgct
      481 accacgatag atttcagtga caattttttc aatcttttct tggacagaaa ggtcattatc
      541 atacaaacgt ttatagttag ctggattttc agcaattgtc ttaacaactg tttcggcaag
      601 tgctactcca ccttctgctc catcagccca gacactagcc aattcaactg gtacatcgat
      661 tgaggcacag agttctttta aggctgcaat ttcagcttct gtatcagata caaattcgtt
      721 aatagctaca actgctggaa taccgaactt acggatattt tcaacgtggc gtttcaagtt
      781 agcaaaacct gcacgaactg cctctacatt ttcttcagtc agagcgtctt tagccacacc
      841 accattcatc ttaagggcac gaagggttgc gacaataaca actgcatctg gagatgttgg
      901 caagtttggt gtcttgatat caaggaattt ctcagcacca aggtccgcac caaaaccagc
      961 ttcagtaaca gtgtaatcag ccaagtgaag ggctgttgtc gtcgccaaaa cagagttaca
     1021 gccatgagcg atattggcaa atggaccacc gtgtacaaag gcaggtgtac cgtaaattgt
     1081 ctgaaccaag tttggcttaa tagcatcctt caaaatcaaa gccaaggcac cctcaacctg
     1141 caaatcacct acagaaacag gcgtacggtc atagcgataa ccaataacga tattcgccaa
     1201 acgacgtttc aagtcctcga tgtccgttgc caagcaaaga attgccatga tttctgaagc
     1261 aactgtaata tcaaaaccat cctcacgtgg aataccgttt agaggaccac caagaccaac
     1321 agtcacatgg cggagcgtac ggtcgttcaa gtccacaacg cgtttccaga ggatacgacg
     1381 ttgatcaatt cccagctcat tcccttggtg caagtggttg tcaatcaagg cagaaagggc
     1441 attgttggca gttgtaatag catgcatatc tccagtaaag tggaggttga tgtcttccat
     1501 tggcagaact tgtgcatacc caccaccagc agcaccaccc ttgatcccca tgactggacc
     1561 aagagacggt tcgcggatag caatcatggt tttcttgcca atcttgttca aggcatccgc
     1621 aagaccaatg gtaagcgtcg actttccttc acctgcaggt gttgggttga tggcagtaac
     1681 caagatcaat ttaccgactg gattgctctc aactgcacga attttatcaa agctgagttt
     1741 agccttgtac tttccgtaca actccaaatc gtcataagaa ataccaagtt tctctacaac
     1801 atcaacaatt ggcttcaact caatactctg tgcgatttca atatctgttt tcattcaaaa
     1861 ttcctctaac ctcttatatg ataattcatt atatcacaaa acaagatttt taacatccta
     1921 aaactctcta aacgttcgta aatatctctg tttttaagac ttttagagtc ctttcttaaa
     1981 ttttatatgg ctttatagtt tgaaactata ataaatcttc gtttttacca aaaatttatc
     2041 actttcattt tacttaccgc ttatttttgt gtacaatagt gctatgaaaa ttttagttac
     2101 atcgggcggt accagtgaag ctatcgatag cgtccgctct atcactaacc attctacagg
     2161 tcacttgggg aaaattatca cagagacttt gctttctgca gggtatgaag tttgtttaat
     2221 tacgacaaaa cgagctctga agccagagcc tcatcctaac ctaagtattc gagaaattac
     2281 caataccaag gaccttctaa tagaaatgca agaacgtgtt caggattatc aggtcttgat
     2341 ccactcaatg gctgtttctg actacactcc tgtttatatg acagggcttg aggaagttca
     2401 ggctagctcc aatctaaaag aatttttaag caagcaaaat catcaggcca agatttcttc
     2461 aactgatgag gttcaggttt tgttccttaa aaagacaccc aaaatcatat ccctagtcaa
     2521 ggaatggaat cctactattc atctgattgg tttcaaactg ctggttgatg ttaccgaaga
     2581 tcatctggtt gacattgcac gaaaaagtct tatcaagaat caagcagatt taatcatcgc
     2641 gaatgacctg actcaaattt cagcagatca gcaccgagct atatttgttg agaaaaatca
     2701 gcttcaaaca gtccagacta aagaagaaat tgcagaactc ctccttgaaa aaattcaagc
     2761 ctatcattct tagaaaggaa aactatggca aacattctct tggctgtaac gggttcaatc
     2821 gcctcttata agtcggcaga tttagtcagt tctctaaaaa aacaaggcca tcaagtcact
     2881 gtcttaatga ctcaggctgc tacagagttt atccaacctt tgacactaca ggtactctca
     2941 cagaatcctg tccacttgga tgtcatgaag gaaccctatc ctgatcaggt caatcatatc
     3001 gaacttggaa aaaaagcaga tttatttatc gtggtacctg caactgctaa cactattgca
     3061 aaactagctc acggatttgc ggacaacatg gtaaccagta cagctctagc cctaccaagt
     3121 catattccca aactaatagc tcctgctatg aatacaaaaa tgtatgacca tccagtaact
     3181 cagaataatc tgaaaacatt agaaacctac ggctatcagc tgattgctcc taaggaatcc
     3241 ctactagctt gtggagacca cggacgagga gctttagctg acctcacaat tattttagaa
     3301 agaataaagg aaactatcga tgaaaaaacg ctctaatatt gcacccattg ctatcttttt
     3361 tgctaccatg ctcgtgatac actttctgag ctcacttatc tttaaccttt ttccatttcc
     3421 aatcaaaccg accattgttc atattcctgt cattattgcc agcattattt atggtccacg
     3481 agttggggtt acacttggat ttttgatggg attacttagc ttgacggtta acacgattac
     3541 gattctaccg acaagctacc tcttctctcc cttcgtacca aacggaaaca tctactcagc
     3601 tatcattgcc atcgtcccac gtattttgat tggtttaact ccttacttag tctataaact
     3661 gatgaaaaac aagactggtc tgattttagc tggagccctt ggttccttga caaatactat
     3721 ctttgtcctt ggaggaatct tcttcctatt tggaaatgtt tataatggaa atatccaact
     3781 tcttctggca accgttatct caacaaattc aattgctgaa ttggtcattt ctgcaattct
     3841 aaccctagcc attgttccac gactacaaac cttgaaaaaa taaaaacaat ctccgctttc
     3901 aaacctgaag gtggagattt tgctctatac agtttctttt taatgaaatc tttactatag
     3961 taaaacgaaa taagaacagg acaaatcgat caggacagcc aaatcgattt ctaacaatgt
     4021 tttagaagta gaggtggact attctagttt caatctacta taacttttaa ctaaaaaatg
     4081 atctataaac ccaagtaaat tcttgcttta ttgtctggtt tattgtacca tactagtgta
     4141 tatgcagtta aaaaggagat tcttgtgaat acacggaaaa agacacaatt tatgacaatg
     4201 acagcccttt taacggctat tgcgattttg attccaattg ttatgccttt caagattgtc
     4261 attccacctg cttcctatac tttggggagc cacatcgcta tttttatagc catgttcttg
     4321 tcgcccttga tggcagtttt tgtcatccta gcctctagtt ttggattttt gatggctggc
     4381 tatcccatgg ttatcgtttt tcgggctttt tcccatatat cttttggtgc tttaggagct
     4441 ctttacctac aaaaattccc cgatacccta gataaaccaa aatcttcctg gattttcaac
     4501 tttgttttgg ctgttgttca tgcccttgct gaagtattgg cctgtgtcgt tttttacgca
     4561 acttctggta ccaatgtaga aaatatgttt tatgttctat ttgtactagt tggatttggt
     4621 acaattatcc atagtatggt agactataca ttagcactag ctgtctataa agtgcttcga
     4681 aaacgccgtt aaaaggaaga actatgacaa aagatcgcaa acaagccctg ctccaactct
     4741 tgaaagaagc tcctaaagcc ctcaatggcc aaagattagc tgagcacttt catgtcactc
     4801 gacaagttat tgttcaagat attgcaattt taagagctga tggatctcca attctatcta
     4861 ccaatcgtgg ttatatctat aaagatgcta atgcaaatac ttatcatcac aaattattta
     4921 aggtcaagca tgaagttgaa gaaattggac gagaattact tgctatcgta gataatggtg
     4981 gtcgcgttca aaatatcttg attgatcatc ctgtttatgg agagattgaa accttactaa
     5041 aactgacctg tcgccgcgac gtgcaacatt ttctagaaca agttgaacgt tctgacttta
     5101 agccaatttc tgagttaaca gatggtattc attatcacct agttgaagcc gagacacaac
     5161 aagacctcca ctatatcgag gaggccttgg atcagcttgg ttatttagta aaggattaga
     5221 aaattttctt ttcccaatcg tcttctgtac ggtggcggta gaaaaactct gacttgtcag
     5281 gagcgtccat catctccatc aaaggtaaca tatcataggc cagatccaag tttggaatct
     5341 ggtctttttg cacccaagaa acttctccct cttctgaaga ttgaagggta ccagagaact
     5401 cagttgcctt ataacaaacg acaatgtagc gcccgcctgt atctagtggc cagttcttga
     5461 taccgaccag ttgaggattt tggatagtca atcctgtttc ttcgtagatt tcacgaatga
     5521 cagactccgc gaaagactcg ccattttcta catggcctcc tggaaaggca taaccagacc
     5581 agcgattggt ttcaggagag cgatactgca tcaccacgcg ctgagtttcg aggtcttcaa
     5641 tcagacaaat gtttgttaaa atcgttaatt gggaacggga cataaattta ctcgctttct
     5701 aatttattaa attttcagac ttggtaccat attagctttt acttttgaga attatttaga
     5761 atagaatacc atccgttttt cctttatagg ctttccaagt tcaagtattt tttcttgtcc
     5821 atcaaaagta aaacccattt tttgatagaa agcaatggcg cgcttgttat ctttcaatac
     5881 ccataagaaa atttcagaaa aatgattaag atcagtcaaa gctgctttca ctaacttttg
     5941 tgcgattcct tttccataat agtcttttaa aacatataaa gcaataattt caccagcttg
     6001 aatagtctca tcacgacagt tgccataact tataaaacca actatcttca caccatccat
     6061 cgcaatcaat gtattttctg gatacttttg actaaagagt cgacatcttt ctaatgtcat
     6121 tgtctcctga aattccgcag gcaaaaggtc atcataagcc tctctccacg tttgccagtg
     6181 aacgaaggat ttaccttcta tctcttcagg agtttccatt gatttgataa taaccgtcat
     6241 ttattttctc ccagtcttct ctcaaaatac catatttaat actgtcaaaa tatttacctt
     6301 gataataacg aacttttgga atatgagctt cttttttcat tcttaatttt tcagcaagtt
     6361 tcatcatacc aatatttcct gaccaagttg tcaaacccag atgctccaac tccaagtaat
     6421 cctgaaacgt cctatctatc cactgcaaca tagcaacttt cccaatacca gtgttccaga
     6481 attttttatc ataaatacca attcccaatt ccatccatct tgtttcttta catacccaat
     6541 aacgcgaaac agtcccaact agtttatcat caacaaaaat accaaggcga tttgagttgc
     6601 ttaaaatgga ttctgatttt tgtagttcga attctttaaa atttgaaaaa tactgataat
     6661 catcataata gggagcatca tactgtttcc aaactggatt aagctgtgaa taagccattt
     6721 cccataaaga catcaaatct tctttcttta actttcttaa ataaatcata ctattcccct
     6781 aatccaaggc cttgacttcc agcatcatat cacgaatctg agctgctagt tcaaagtcaa
     6841 gcacttcaac tgcttcttgc atttgtttct caagcttttt gactagttct ttgcgctctt
     6901 gtttgttgag gctattgata tcgacttcct tgtcttcttc cttagcaact gccttggtca
     6961 cagcaatcaa gtcacggatt tctttcttga tggtttgtgg aacgatacca tgttcttcat
     7021 tataggccat ctggattttg cgacggcggg cagtttcatc gatagcacgt tgcatagact
     7081 gggtaaccgt gtccgcatac atgataacat gaccttcgct attacgtgca gcacgtccaa
     7141 tggtctggat gagtccacgt tcgttgcgaa ggaaaccttc cttgtcagca tcgagaatag
     7201 ctacgaggct cacttcagga acgtcaattc cttcacggag caggttaatt ccgaccaaga
     7261 catcaaagac acccaagcgc aggtcacgga taatctccgt ccgttccaag gtcttgatat
     7321 ccgagtgcat gtacttgacc ttgataccca tttccttgaa gtagtcggtc aaatcctctg
     7381 ccattttctt ggtcaaagtt gtgataaagg tacgctcatt tttttcaacg cgggcattga
     7441 tttcacccaa gaggtcatca atctgtccca tagtcggacg gacttccacc tctggatcca
     7501 agagtcccgt tggacgaatg atttgctcaa tcactgtctc ggtctgttca ttttcatagt
     7561 caccaggtgt cgctgaaacg taaacaatct gatgaacgtg actctcaaac tcctcccgac
     7621 ggagaggacg attgtccaaa gcagacggca aacggaaacc ataattaacc agcatttctt
     7681 tacgcgaacg gtctccattg tacatgccct tgatttgccc tatggtcata tgactctcgt
     7741 caatcataat caagaaatca tctgggaaga agtcgagaag cgtataagga ggctctcctt
     7801 cgctccgtcc atccatgtgg cgagaataat tttcaacccc attggtatag cccatctcac
     7861 gcaacatttc gatatcatac tctgtccgct gtttcaaacg ctgggcttca agcagtttac
     7921 cttccttttc aaagacagct aattgttctt ccaactcggc ctgaatcttt gcaatggcaa
     7981 cttccatgtg gtcgtcattg gtcacaaagt gtgtcgctgg gaaaatcgct aaatgatcca
     8041 cttctcccaa cacctgacct gtcagagcct caacttcacg aatacggtca atttcgtctc
     8101 caaaaaattc tactcgaaag gcatgttcat ctcgggaagc tgggaaaatc tctaccacat
     8161 ccccacgaac gcgaaatctt ccgcgttgga aatcaatatc attacgttca aactgaatat
     8221 cgaccaagtc attcaagagt ttatcacgag aaatctctag accaggacgg agactaacga
     8281 cactatcagc gtattccttg ggcgaaccca aaccatagat acaagagact gaggccacga
     8341 caataacatc attacgctcc aaaagggctg aggtagctga gtggcgaagc ttgtcaatct
     8401 cgtcattgac agaactatcc ttctcaatat aggtatcgct agaagggaca taggcctctg
     8461 gctggtaata atcatagtag gatacgaaat actcaactgc attttcaggg aaaaattcct
     8521 taaactcccc atagagctga ccagccagag ttttattgtg ggcaataacc agagttggtt
     8581 tattgacttt agaaatgacc tgactcatag tataggtctt ccctgttcca gtcgccccca
     8641 tcagaatctg agctttttct cccccctcaa tgttatccac caactgctcg atagcttggg
     8701 gttgatctcc tgatggttga tattttgata ctagtttaaa ttgattatct gtaatgtgat
     8761 tgatcataag aatcctctct ccatgtgcta ttcctatttt agcatactta tagtattttc
     8821 acgaagaaaa ggacggaccg aagtcacatc ctttcatttt ctttctttaa tttattagca
     8881 agatagacaa tcagcagtag taataccaga cttgtcataa tggaaccttc tatgccaaaa
     8941 gaaccacctg atagccaatc ttgattgcct tgtggtaaaa aggtcatcag agacgttcct
     9001 gacggttgac cactaactaa aatcccaaag agattaccct gagcaaaatt ccaagcacca
     9061 tgaatacctg caacacccca aactgtatca gttttgagaa ggtaaagagc catggcaact
     9121 ccgaataaaa agagatttac tagagataga ggggtgagac cagaattgcc catatgaagc
     9181 agggtaaaga acaggctaga tataagaata gctagtttta gattggttct tgaggccaat
     9241 tgaggaagta gccaagcacg ggccaccact tcttctgctg tcccctgtaa aatccaaaat
     9301 gggatagtaa agacgacaaa ggcaagagaa taaggattca agtgaatgga ttccaaacga
     9361 tattgaccta agaccactaa acctaacaag gtcagaagaa aaagtgccag gcctagacta
     9421 aatcctttca gaagattgct gaggaaattc tctctataaa atcccaaggt tcgaataggt
     9481 cttttctcaa cttttcttgt ccatctgaac acagtgttaa gaataaaacc aaaggccagt
     9541 aatcctaaaa ttaaacgaaa gtcacttgtt ttatccgtca agctctgatg caaggtctgc
     9601 aagtagcttg caagattttg accagcctct ccaaaatttc tagcaagtcc gatgagagct
     9661 aacaagccaa taccatacaa agtaaaagcc acaaactctc ctatgaagac aaaaacaaag
     9721 gctaacaagg ggcttgtgta aatgtttaaa ctcatttttg aagcttggaa gtctttaaat
     9781 attcttttgt tcttcatatc cccgtcctct tttc
//
LOCUS       DAVHEW010000081         9687 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVHEW010000081 DAVHEW010000000
VERSION     DAVHEW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.081"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            198..293
                     /locus_tag="VVL53_001683"
                     /pseudo
     CDS             198..293
                     /locus_tag="VVL53_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(546..1166)
                     /locus_tag="VVL53_001684"
     CDS             complement(546..1166)
                     /locus_tag="VVL53_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4183828.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPGSIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1166..1648)
                     /locus_tag="VVL53_001685"
     CDS             complement(1166..1648)
                     /locus_tag="VVL53_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU4183829.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1648..2490)
                     /locus_tag="VVL53_001686"
     CDS             complement(1648..2490)
                     /locus_tag="VVL53_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU4183830.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2585..3385)
                     /locus_tag="VVL53_001687"
     CDS             complement(2585..3385)
                     /locus_tag="VVL53_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU4183831.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSAQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3529..4908
                     /gene="glmU"
                     /locus_tag="VVL53_001688"
     CDS             3529..4908
                     /gene="glmU"
                     /locus_tag="VVL53_001688"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU4183832.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVIVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4918..5463
                     /locus_tag="VVL53_001689"
     CDS             4918..5463
                     /locus_tag="VVL53_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4183833.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5475..5789
                     /locus_tag="VVL53_001690"
     CDS             5475..5789
                     /locus_tag="VVL53_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU4183834.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5806..6498
                     /locus_tag="VVL53_001691"
     CDS             5806..6498
                     /locus_tag="VVL53_001691"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU4183835.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6575..7066
                     /gene="rocS"
                     /locus_tag="VVL53_001692"
     CDS             6575..7066
                     /gene="rocS"
                     /locus_tag="VVL53_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU4183836.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7164..7754
                     /locus_tag="VVL53_001693"
     CDS             7164..7754
                     /locus_tag="VVL53_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU4183837.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNATKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7763..9526
                     /locus_tag="VVL53_001694"
     CDS             7763..9526
                     /locus_tag="VVL53_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU4183838.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2991 a         1683 c         2069 g         2944 t
ORIGIN      
        1 ataattttaa agactttttg accagcctca ttttttaact tgatactcag tgaaaagcaa
       61 agattaaact aggaagctag ctgtaggctg ctcaaagaac agctttgagg ttgcagataa
      121 aacttgtgag gtaaccaaca tatataatgt gaagctgacg tggtttgaat agattttaga
      181 agggtatgag tctggaagtt ttaatggata atgcaagatt ccatagaatg ggtaagctag
      241 agttcttatg tgaagagttt gggcataaac ttttaccttt tcctccctac tcatcttagt
      301 atagaaaagt gaatctaaaa tagtacataa ctgcttctaa aacattctta taaattgatt
      361 taaattctca aatcatatta ttcagttctt atttcatttt gttctacaat cctgttgaga
      421 agacacgtgt tcatatcaaa aaggtattgg caagttgcaa taccttttta cgaggctctg
      481 ttgtcttatt tttgtttcaa ctgactatat ctcctatggt tctagttcag aaggctaggc
      541 tataattatg attgataaga agtatcattc caagtattga gagtgaatgt ttcaaaatca
      601 tgggtttcta taatggtcag gctggcattt gctagaccgc catctttacg aagaagtggt
      661 tctttatagc ctaggagagt acgaagactg gcagtaagat tggcgccgtg tccgacaatt
      721 agaatacgct cagctggact atcttttaat gatttgataa attggatggt ccgctgagtt
      781 gtactataga gggattcggc tccgaacatt cgagtgtcaa attgagcaag atttgaacga
      841 aaagcctgga tttgttgcgg gtaaatagct tccagggttg caattttcaa accttctaac
      901 ttcccaagtt gccattcacg gagattagga acgatttcta aagaacaggg ggtatggagt
      961 tgactttgga taatctcagc agatttgacc gctcgaggta aatcacttga ataaatctga
     1021 tcaaaaggaa tttccttgag atactggcca agtcgtttta gggtttcaat ggatccagga
     1081 agaagaggag aatcaccgct agcaccttga aaacgacctt cttggttcca gagggtacga
     1141 ccgtggcgga caaagtagag tttcattact tgatgtcctc caaaatatct acaaagtctg
     1201 cctttacaaa gctagccaag tcttgtggcg cgacgataat gctgtgtccg acttcgcctg
     1261 cagagacaat cattcgatcc aaatctagag caattttatc gataaaaatg ggataattgt
     1321 gtttctgacg aattccgaca ggattattgg ctccatgaat gtaaccagtt gttttttcta
     1381 agtccttttg tggaatcatg ctcacttttt tattgccaga aattttagct agtttctttt
     1441 cagacaagtg ctgagtgata gggacaattc cgataatcgg tccggtcttg tctcccaaaa
     1501 gcgccaaggt tttgaaaatc tgatctcgtt cataaccttg aggaagctct ccttctaggg
     1561 cattgatttg aatcccctga tgagggatag ctgctttaga taggatttgt tccaccaatg
     1621 tttttttgat tttaactttt tttgccatta tttatattta tcctccaatt gactcatcca
     1681 aataccaagc cagattccca gcgcaaagaa gaaggcgatg atgacataac cgacaagtga
     1741 aagtcctgtg tattggatac tttcagcgtt tcctgcattt ggaattaaga tcaaaagggt
     1801 acttgatagg acgataccga tgatgaaatg atagacgcgt gagtggtagt tgtttaaggc
     1861 ataatccatc aattttgaaa aaacgatgag agttgcacct gcaccaattc caatcggaaa
     1921 gaaggttccc aagagatcaa aagttttaaa accagtcaac ataggagcat agagtcccaa
     1981 aatcaaaagt aaattcgatg ggctgaggcc aggaaccaag acgccaaggg ccaatagtgc
     2041 gcctgctagg acgaagttaa gaaagctggc gcttaaggtt ccaacgacaa aatttaaggc
     2101 atagagtcct aatccagaaa tgataaaggt tgtccataac caagctaaat caatcttgtc
     2161 tcggtcagat tctcgagttg attctttgag gaggctagga actgtaccga taatagctcc
     2221 cgcaaagctc cataatacaa agacctgata attttcaagc aggtattcaa tcgggtagga
     2281 aaataagccg attcccagaa gcataccgat ggcaactgga ataaagtaca aaacattttc
     2341 tttaaggtct ttaaagggat gggccagaaa gccaatcatt cgttcataga ttcctaagat
     2401 tgctgctaga acccctccgg aaattcctgg taggataaat ccaagagcaa ttacaatccc
     2461 tttaataacg cgtgctaacc atgagagcat atatttcctc caactatttc tatttcaaaa
     2521 aatccttact atattgtatc acaatcagac acaaaaagaa aaagcaaatg ataaacaaat
     2581 gcttttaagg ttttaaaaaa agttttcgaa aggtttcttc tttatttttt aagggagaga
     2641 taacgttgat atctaaatcg tggtcaaagc cggcaatttt tcctttagat gtgtattggt
     2701 gaatatcata atctaaatca gttttaggac tgctctccaa aaatcctgag tctgagccgt
     2761 aggacggaat ccaaacagag gtaaacttgc ctgtatcaat actgtgttct tccatgaagt
     2821 agacaccaac gtagatgccg atgtttttag cacctagtga tgctagtttt gctcgaaagt
     2881 tttcgacacc ttcgttcata ttagacatgg ttttgtcttc cacgtcaagc caatagtaac
     2941 tagggctgta aggagaggca gcattgtaga aaacttcggc agccttttcc atttcttggg
     3001 cactttttcc agctacataa gcgtagacag caactgggac attccgcttt tgaagttcag
     3061 tgatatgact cttataggcc ttgtctattc cattgataaa tgaagcatca ttttcttttg
     3121 tcgtttgagc accactgtga acacgaacaa tagcacctga aatattttgt gagagggcat
     3181 cgtagttgat ttcctcagga cgctgccagc cagagaggtc aataatcggt ttgtctaagt
     3241 gtttcaaagc ctgtgcttca atctgtgcta tattggattt tgttttaaac gattggctgt
     3301 cattaagtgg gcgattgatg attaaaatga acatcataat ccccaaaaaa ctaaataaaa
     3361 taagtggatg aatttgtttt ctcatatctt ataattctac cctaaaaatc aaaaaaaatc
     3421 aaaaaaatgg gttaaggaag agactttaga gcattttttc attcaagagt gcggaatgat
     3481 ttgaaatatg gtataataaa agggaatttc tacagaaaag agaagattat gtcaaatttt
     3541 gccattattt tagcagcggg taaagggact cgcatgaaat ctgatttgcc aaaagttttg
     3601 cacaaggttg cgggtatttc tatgttggaa catgttttcc gtagtgtggg agctatccaa
     3661 cctgaaaaga cagtaacagt tgtaggacac aaggcagaat tggttgagga ggtcttggct
     3721 ggacagacag aatttgtgac tcaatctgaa cagttgggaa ctggtcatgc agttatgatg
     3781 acagagccta tcttagaagg tttgtcagga cacaccttgg tcattgcagg agatactcct
     3841 ttaatcactg gtgaaagctt gaaaaacttg attgatttcc atatcaatca taaaaatgtg
     3901 gccactatct tgactgctga aacggataat ccttttggtt atggacgaat tgttcgtaat
     3961 gacaatgctg aggttcttcg tattgtcgag cagaaggatg ctacagattt tgaaaagcaa
     4021 atcaaggaaa tcaacactgg aacatacgtc tttgacaacg agcgtttgtt tgaggctttg
     4081 aaaaatatca ataccaataa cgctcaaggc gaatactata ttacagacgt cattggtatt
     4141 ttccgtgaaa ctggtgaaaa agttggcgct tatactttga aagattttga tgaaagtctt
     4201 ggggtaaatg accgtgtggc gcttgcgaca gctgagtcag ttatgcgtcg tcgcatcaat
     4261 cataaacaca tggtcaacgg tgttagcttt gtcaatccag aagcaactta tatcgatatt
     4321 gatgttgaga ttgcttcgga agttcaaatc gaagccaatg ttaccttgaa agggcaaacg
     4381 aaaattggtg ctgagactgt tttgacaaac ggtacttatg tagtggacag cactatcgga
     4441 gcaggagcgg tcattaccaa ttctatgatt gaggaaagta gtgttgcaga cggtgtgata
     4501 gtcggtcctt atgctcacat tcgtccaaat tcaagtctgg gtgcccaagt tcatattggt
     4561 aactttgttg aggtgaaagg atcttcaatc ggtgagaata ccaaggctgg tcatttgact
     4621 tatatcggaa actgtgaagt gggaagcaac gttaatttcg gtgctggaac tattacagtc
     4681 aactatgacg gcaaaaacaa atacaagaca gtcattggaa acaatgtctt tgttggttca
     4741 aattcaacca ttattgcacc agtagaactt ggtgacaatt ccctcgttgg tgctggttca
     4801 actattacta aagacgtgcc agcagatgct attgctattg gtcgcggtcg tcagatcaat
     4861 aaagacgaat atgcaacacg tcttcctcat catcctaaga accagtagga gcctatcatg
     4921 gagtttgaag aaaaaacgct tagccgaaaa gaaatctatc aaggaccaat atttaaactg
     4981 gtccaagatc aggttgaatt accagaaggc aagggaactg cccaacggga tttgattttc
     5041 cacaatgggg ctgtctgtgt tttagcagta acggatgaac aaaaacttat cttggtcaag
     5101 cagtaccgca aagctatcga ggctgtctct tacgaaattc cagccggaaa attggaagta
     5161 ggagaaaaca cagcccctgt ggcagctgcc cttcgtgaat tagaggaaga aacagcctat
     5221 acagggaaat tagaactctt gtacgatttt tattcagcta ttggcttttg taatgagaag
     5281 ttaaaactat atttagcaag cgatttgaca aaagtggaaa atccgcgtcc gcaggatgag
     5341 gatgaaacct tggaagtcct tgaagtgagc ttagaagaag cgaaagaatt aatccaatca
     5401 ggtcatatct gtgatgccaa gacaattatg gctgttcagt attgggagtt gcagaaaaaa
     5461 tagaggaggt cagtatgggt aaatctttat taacggatga aatgattgaa agagctaata
     5521 gaggcgaaaa aatttcaggt cctcctttgc tagatgataa tgaggaaact aagattttac
     5581 caacctcttc ttcccgtttt ggttatgcca atcctaagga tcatggtttt agccaggaaa
     5641 ccttgaagat tcaggtcgaa ccatctattc ataaaagccg tcgtattgaa aataccaaga
     5701 gaaatgtctt caattctaag ttgaataaaa tcttatttgc ggtcatcttt ctcttgattt
     5761 tgcttgtttt agcaatgaaa cttttgtaat agaaaaggaa ttgaaatgaa aataggaatt
     5821 attgctgcta tgccagaaga actggcttat ctggtccagc atttagataa tgcccaggag
     5881 caagttgttt ttgggaatac ctatcataca ggaaccattg cttctcatga agtcgttctt
     5941 gtagaaagtg gaattggtaa ggtcatgtct gctatgagtg tggcgatttt ggctgatcat
     6001 ttccaggtgg atgcccttat taatacgggt tcagctgggg cagtagcaga aggtatcgct
     6061 gttggggatg tcgtgattgc tgacaaatta gcctatcatg acgtggatgt cacagctttt
     6121 ggctatgctt atggacaaat ggcgcaacaa ccgctttatt tcgaatcaga caaaaccttt
     6181 gttgctcaaa tccaaaagag tttatctcaa ttggaccaaa actggcatct tggtttgatt
     6241 gctacaggag atagttttgt tgcaggaaat gacaagatag aagcgattaa gtcccatttc
     6301 ccagaagttt tagccgtgga gatggagggg gcagctattg ctcaagcagc gcatgccctc
     6361 aatctcccag tcttagtcat cagagctatg agtgacaatg ccaaccatga agcaaacatc
     6421 ttttttgatg agtttattat cgaagctgga cgtcgctctg cccaagtctt gttgaccttt
     6481 ttgaaggctt tagattaagc ggaaatttga cagtttttct agcttatgat aagatttaag
     6541 taaagaaaag ctagaaaacg tttcagagga tattatgagt attgaaatga ccgtcagtga
     6601 gattgcagag gtcttaggat tatctcgcca agcaatcaat aaccgtgtca aagaattacc
     6661 agaagaagac acagataaaa atgacaaagg ggtaacagtt gttaccagaa gtggcttgat
     6721 taagctagaa gaaatctata aaaaaacgat ttttgaagat gaacctgtca gtgaagatgt
     6781 caaacaacgt gaactgatgg agattctagt ggatgagaag aatgcagaaa ttcttcgtct
     6841 ttatgaacaa ttaaaagcca aggatcgtca gttatcagaa aaagacgagc agatgcgtat
     6901 caaagaccgt cagattgctg agaaagacaa acaattagat cagcaacaac aattgaccct
     6961 tcaagccatg aaggatcaag aaaatcttaa gctagagctg gaccaagcaa aagaagaagt
     7021 ccaatccact aaaaaaggct tttttgctcg tttatttgga ggataatcaa ggagctgttt
     7081 aggttaaatg ctaacctgat ggcttctttt gtttgtgaat agtatagttg attagaacag
     7141 agacagtaga ggagaggtac agaatggaac gattagaaga ttacattgct tttgatttag
     7201 aatttaatca gcatgaagga caaacacact tgattcaagt atcggcagtg cgttttagag
     7261 atggtcggga aatcgatgcc tatgattcct atgttcatac cagcgtccct ctaaaaagct
     7321 ttatcaatgg tttgaccggg attacagctg agaccttaaa agatgctcct ccagttgaga
     7381 aagtcttaga agaattccaa gagtttgtgg gttctttgct gatgataggc tacaatgcta
     7441 ctaaaagtga tttacctatt ttagcagagc atggtttaga ctatagccaa cagtacaagg
     7501 tggatttgta tgatgaagct tttgaacgtc ggagttcaga tttacatggt attgccaatc
     7561 tcaaattgca aactgttgcc tcatttcttg gatttcaagg tcagtctcat aatagtttag
     7621 aggatgctcg gatgacggcg cgtgtttacg aatccttctt agagtcggat caagctaggg
     7681 aattgttaga gacagaaagt agcttatcta acaatccttt tggtggcttg gacttgtctc
     7741 aattatttga ctaggagtgc ctatgaaacc tgaaaaacct aaaaaattgg gtcttaaaca
     7801 gatttacctc aatcttgata aaatcctctt tctatttttc ttgattttta tgatagtgga
     7861 gtatttgtgg gtaccattca attcatggtt ggctagcttc ttattagatc agacaggtta
     7921 tctttttatt tcctataaca atgtcttagc catccttact cattctccat tgattagtct
     7981 agctttttta gccttgattg taattaacct tctggttgct tattttcaaa taggactcct
     8041 ctttattggt gctcgtcatc tcctctataa tgaaaagcga aggctaattg agtatatccg
     8101 caaggttttc catgaaagtt ttggatttat gaaaaagcta acttgcgcta aagccttgtt
     8161 tatctttttc tatatagcta tgctgtttcc ttttatacgg aaaatgctga agatttatta
     8221 tcttaataaa atcgtcatcc cagagtttat ccaagcttat ttagaagata aatattggat
     8281 gtggtggctg agcatcctac ttctatctct tatctttctc tatgtctctg tcagattgat
     8341 gtttgccctt ccgaagattg tatatgatca gctgactgtt cgagaagcag tgatgtttag
     8401 cttggagaaa accaagaaga gagttacttt ttatgcttgg aatttatttt acatcttgct
     8461 caaagcacat ttattatttt atcttccttt gattccttta ttattggctc aaactttggt
     8521 agatgatatc actcagagag agtctctgat tcttggtatt ttgaattttg tcgtgattaa
     8581 aaatctctat tatatggctc tgacttattt tttggttaaa tttgtttcat ttttgacagg
     8641 aaaggagctg gatatgcttc ctaggagaga aaaagatcac atcgtgcgat ggggtgtcat
     8701 gacttgtgcc agtcttttct ttgccttaga aggttatatt tatctggagg ctcccatggt
     8761 tcatctacct caacttattt ctcaccgagg ggtttccaat gcaaatggga ttcaaaatac
     8821 agtagagtcc ttggaaacta cagcacaact caaaccagac ttggtggaga cggacgtgca
     8881 ggaaacaaaa gatgggcagt ttgtcatgat gcatgatgct aacttgaaaa atctagcagg
     8941 tatcaataaa agtcctcaag acttaaactt ggaggagctt aaagggattg atatttttga
     9001 aaatggctac cagactaaaa tttcaagctt tgaagattat ctcagtcgag ccaacgaact
     9061 tggtcaaaaa ttactaattg aaattaaaac cagtaaaaaa gatagtccag acatgatgaa
     9121 ccgcttttta gcccgttatg ctgcaaagct caagatttat ggacatcaaa tccagtcttt
     9181 agactaccat gttgtcgaaa aagtaagaca gtatgatgcc gaactaccag tttatttcat
     9241 catgccctac aattctgtct ttcctaaaac aagagcgaca ggatatacta tggagtactc
     9301 aaccttggat gaatattttg taagcaaact atggacaacg gatcagaaac tttatgtctg
     9361 gactgtcaat gattcagaag ctatcagcaa atctcttcac ttaggagtag atggagtgat
     9421 tacagatgat ttggaaaaag ttcagcggga aatagaagta gcccaagaag atccagaata
     9481 tacggatttg ctcttgaaaa aagcattgga attctttgaa ttttagtaat aaaaaagagg
     9541 aacggttgtt cctcccatga taggtaagta ttcttgtgaa tgcttgcttt tttgtggttg
     9601 caatccgttt acgagaattg tttgtattgt tcttgtttca atttacttta tagtgctttg
     9661 aagttggaat gctacactat aacttat
//
LOCUS       DAVHEW010000082         9655 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVHEW010000082 DAVHEW010000000
VERSION     DAVHEW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.082"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..890
                     /locus_tag="VVL53_001695"
     CDS             <1..890
                     /locus_tag="VVL53_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4183839.1"
                     /translation="AHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMS
                     FIAQDFNNLNIITVLKGRTQAVIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFP
                     CAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKR
                     FYRPTFRMHLTNKEILNKLLSYSQNLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQ
                     VHPIFQTVFKTFLKDKKKIVNTLQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKR
                     IFIALNIKKERTKFVLSRA"
     gene            complement(941..1876)
                     /gene="dapA"
                     /locus_tag="VVL53_001696"
     CDS             complement(941..1876)
                     /gene="dapA"
                     /locus_tag="VVL53_001696"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU4183840.1"
                     /translation="MSYQDLKKCKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(1932..3008)
                     /locus_tag="VVL53_001697"
     CDS             complement(1932..3008)
                     /locus_tag="VVL53_001697"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU4183841.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(3350..3422)
                     /locus_tag="VVL53_001698"
     tRNA            complement(3350..3422)
                     /locus_tag="VVL53_001698"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3387..3389),aa:Thr,seq:ggt)
     gene            complement(3522..4031)
                     /locus_tag="VVL53_001699"
     CDS             complement(3522..4031)
                     /locus_tag="VVL53_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU4183842.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(4029..4125)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            4154..4585
                     /locus_tag="VVL53_001700"
     CDS             4154..4585
                     /locus_tag="VVL53_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU4183843.1"
                     /translation="MKKPIQKFFNNEILAYLFFGIVTTLVAILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            4667..5044
                     /gene="mscL"
                     /locus_tag="VVL53_001701"
     CDS             4667..5044
                     /gene="mscL"
                     /locus_tag="VVL53_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU4183844.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTDEKKPTELEVLQEIKALLEKK"
     gene            complement(5086..6180)
                     /gene="hemH"
                     /locus_tag="VVL53_001702"
     CDS             complement(5086..6180)
                     /gene="hemH"
                     /locus_tag="VVL53_001702"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU4183845.1"
                     /translation="MKKAILMMTFGSPEEIIFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGSEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            6335..7558
                     /gene="pepT"
                     /locus_tag="VVL53_001703"
     CDS             6335..7558
                     /gene="pepT"
                     /locus_tag="VVL53_001703"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU4183846.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(7594..8223)
                     /locus_tag="VVL53_001704"
     CDS             complement(7594..8223)
                     /locus_tag="VVL53_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183847.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            8188..8409
                     /locus_tag="VVL53_001705"
     CDS             8188..8409
                     /locus_tag="VVL53_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183848.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(8486..9027)
                     /locus_tag="VVL53_001706"
                     /pseudo
     CDS             complement(8486..9027)
                     /locus_tag="VVL53_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(9131..9655)
                     /locus_tag="VVL53_001707"
                     /pseudo
     CDS             complement(9131..9655)
                     /locus_tag="VVL53_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         2900 a         1970 c         1733 g         3052 t
ORIGIN      
        1 ttgcccatca gctttccatc tcaacttcaa ctgttattcg taagctcaat gactttcact
       61 ttaaacatga tttttcttgt cttcctgaga ttatgtcttg ggatgagtat gcttttacaa
      121 aaggaaagat gagcttcatt gcgcaagatt ttaacaatct caatatcatc actgttctta
      181 agggaagaac acaagctgtc atccgaaatc actttcttaa atatgataga gccgtccgat
      241 gtcgcgtcaa aatcattact atggatatgt ttagtcctta ctatgactta gctagacaac
      301 ttttcccgtg tgctaaaatc gtgttggatc gctttcacat tgtacaacat cttagccgtg
      361 ctatgagtcg tgtgcgtgtc caaatcatga atcagtttca tcgaaaatcc catgaataca
      421 aggctatcaa gcgctactgg aaactcattc aacaggatag ccgtaaactg agtgataagc
      481 gattttatcg tcctactttt cgtatgcact taaccaataa agagatttta aacaagcttt
      541 tgagctattc acaaaacttg aaacatcact atcagctcta tcaactcttg ctgtttcact
      601 ttcagaataa ggaaccggag aaatttttcg gactcattga ggacaatctg aagcaggttc
      661 atcctatttt tcagactgtc tttaaaacct ttctaaagga caaaaagaaa atcgtcaaca
      721 cccttcaact accctattca aacgccaaat tggaagcgac caataatctc atcaaactta
      781 tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt caaaaaacgg atttttatcg
      841 ctctgaatat caaaaaagaa aggacaaaat ttgtcctttc tcgagcttag ctgacttcaa
      901 cccactacag ttgacaaaga gccggatttt attgtcttta ttagtaatct ggtcttaaga
      961 cccctgttac agttgcctta gttgcttcgt agtcgccatc tacgacaacc ttgataatgc
     1021 gtttggcatc ttcttctggt gctggaacaa gaggtagacg agtgggtcca gcttcaaatc
     1081 ccatatagtt aagaattgcc ttaactggag caggacttgg ataagagaag agagcattaa
     1141 ccttaggaat gaatttacgc tgaattgctg cggctttctt catatcgctt tctgcaatcg
     1201 cagtaaacat ctcgtgcatt tcatccccat ttgtatgaga ggcaacagaa ataaccccat
     1261 ccgccccaag gttcatggca tggaaagcat ctccatcctc acctgtataa atcaagaact
     1321 cttcaggctt gtgctcaatc aagtaagcca tattagccaa gctagtacat tctttgacac
     1381 cgataatatt tggatggtca gccaagcgaa gcatggtttc tggagtcaat tcgacaacta
     1441 cacgccctgg aatgttatag ataataattg gtaggtcaga agcatctgca atagtcttaa
     1501 agtgctgata catcccttct tgagaaggtt tgttgtagta aggaacaata gcaagcccag
     1561 ctgcgaaacc accaaattcc gctacttctt tgacaaactc aatagagtca cgcgtatcat
     1621 tagtacctac acccgcaatc aaaggaacgc gtccattgac aaccttttgt acagccgcaa
     1681 acaactccaa ctcctcatcg tgggtcaaag ttggactctc agcagtcgtt cctgcgagaa
     1741 gaattccatc cgtatgatgg gccaataaat gctcaatcaa ggctggaata gcatcaaagt
     1801 taatggaacc atcctcatgg aagggggtaa taaaggctgt aatgatttta cattttttta
     1861 aatcttgata agacacgaaa acacctctct atttcaaaga aggagttcat ctgaactcct
     1921 aaacgatatg actattttaa ttcaaatttc aattcggctg ttggacgaac caatccacgt
     1981 tcatgaagag tttcagcaat ctgaactgag ttccaagcag cacctttgag aaggttatct
     2041 gaaacaaccc acatgtgaat tcctttttct gcatccaagt ctttacggat acgaccaaca
     2101 aaggtatcac gcgaaccaac tgcattgata gcttgaggat agatttgatg agctacatca
     2161 tcttcaagaa cagcacctgg gaaggctgcg atagctgctt ttacttcttc gattggagcc
     2221 acttcttttg tttcgatata aacagactca gagtgagctg acaagactgg aatacgcaca
     2281 catgttgcag atactgcaat gctatcatct tccataattt tcttagtttc cttggtcatc
     2341 ttcatctctt cgtacgtgta atcattatca gtgaaaacat caatttgtgg aagagcgtta
     2401 aaggcgatag gataatgttt cttgtcacca cctgaaggca agatttccgc atgcaaatca
     2461 cgtggtttca caccatcatt caagacttca cgaagttcac gttgtgtctc aagaattgct
     2521 cccataccag cacctgaaac ggcttgataa gttgaaacaa tgatacggtc caagccccat
     2581 ttttggcgaa ccggctcaag agccaccatc atttgaattg ttgaacaatt agggcaggca
     2641 atgattccgt tgtgagcatc aagtgcatga gcattgacct ctggaacaac caaaggaaca
     2701 tctggatttt gacggaaata agatgtatta tctactacta ccacgccagc ttttactgcg
     2761 tatggtgcat acttagctga tgtagaacta cctgctgaaa agagagcaat atcaactcct
     2821 tcaaaagctg tttcagtcgt ttcttcaatt gtaatatctt gatctttaaa tttcaatgac
     2881 ttacctgctg aacgtgcaga agcaaggtaa cgaattttat cgattggaag tgttgattct
     2941 tccaacattt ttatcatctg agcaccgaca gcacctgtcg cgccgactac agcaactgta
     3001 tatcccataa ataacctctt tcggaatttt ctaaaaattt ctataataga agtattatac
     3061 tactttttcc atgaattgca aagctttttc atcatttttt ggaaaataaa aatcccagtc
     3121 gctagactag ggactaagag gcatcttcat gtgatgagca ggttcacaca actcatcaag
     3181 gtccgctcct gcgttatgac ctcctcatgc tcaatagtag tccgaagact acctatcgac
     3241 tcatcgcata ttctatatta ctagagatac ttgattttgt caataagaaa tttttcagat
     3301 cgagtataac aaaaaactct cgtattcaag agtttcccta tacatatcat gctaattgcc
     3361 gggattgaac cggcgacctc atccttacca tggatgcgct ctgccaactg agctaaatca
     3421 gcttacttaa gaagtatact atattcagaa aaacttgtca agtttcctta gaaattttcc
     3481 ataggaaaaa gccaggtaaa aactacctag cttatcttct tctatttact taaatcaatt
     3541 cgacgaccaa ttttaccaat aataatcgct ccaataatca atgaagcaaa ttcaccgatt
     3601 cctgttgtaa accaagtaag gaagaatggt aattctgcta caatatttaa ctctgcagcg
     3661 atagtaaaca tggaaattga aaagaggatt gaaaagaaga aatgatcttt tcgaatcaat
     3721 ccgttaaaga ggtaatcctt gctatatttt gcaaaaagcc aaacacctaa actgaggaat
     3781 actaaggttg aaccaccacc aacaaagaca tctagcagtc cgaagctaaa gaaattagca
     3841 atcatacaac cgatggtaac tccgatgatg tacttaggat tgtaaaaagc cataaagttc
     3901 atcatttctg aaatacgaaa ctgataagca ccatagctga tggcatttag gggcggtgtg
     3961 acagtcaaaa cgacgtagat agcagcgacg attgcaatat ctgcaacatc acgaatagtt
     4021 aattttttca tcttttctcc tttggcggtt atccgcgtgt aatatgcttg gtgaaagaag
     4081 ctaagcacca agggttgatt cattcaacct tactagtata gcacaatagc ctgctttatg
     4141 ctatacttaa agcatgaaaa aacctattca aaaatttttt aacaatgaaa tcttagccta
     4201 tctctttttt ggtatagtga ctactcttgt tgctatacta tcacgattag tcatttatca
     4261 gctaagtcat caggaactcc ttgctacggc cttggcaaat attatcggta tcctatttgc
     4321 ctttatcaca aatgatacga ttgtatttaa gcaaaaaaag aaaaatagac ttatccgttt
     4381 agtgaaattt tctcttgccc gcctttctac ttttctgtta gacttgtttt taacttttct
     4441 ctttgtaacc caatttcctc acatcatagg gcaattcgta actgaaaaca ttgataagat
     4501 aaatgctatt gaaacaataa ttgcacaaat attcattatt atcatcaatt atatcttgag
     4561 caaggtctat atttttagaa aataatgctc catttatgag ccttttaatt gccttctata
     4621 atgatttagg atacaatgaa acacgaaaca ttgaaaggaa aacagtatgt taaaaaatct
     4681 aaaatcgttc ttgcttcgag gaaatgttat tgaccttgct gtcggtgttg taattgcctc
     4741 tgcttttggt gctatcgtta cttcacttgt aaacgacatt atcactcctc ttattttaaa
     4801 tcccgctttg aaagctgcta aagttgaacg tatcgctcaa ctttcttggc atggagtcgg
     4861 ctatggtaac ttcttaagtg ctattatcaa ttttatcttt gtgggtaccg ccctcttctt
     4921 tattatcaag ggcattgaaa aagcacagaa gctgactggc ataaaggaag aaaaaactga
     4981 cgaaaaaaaa ccaaccgaat tggaagtcct tcaagaaata aaagctctcc ttgagaaaaa
     5041 ataatcgatt aaaaggatct agcgcaaagc taaatccttt tttctttact ctttgggtaa
     5101 cttgcctgct ttttcaagca tttttttgat aagataaggc atcttaacat tttcacgacc
     5161 ttttttctca atcaattttt tcacaaattc tggcatttgt aaatcttgag attcagccaa
     5221 aatgtttttc gtctcgtctg aaggaactaa ctcatcaaag gtctcttctt ctgggagaaa
     5281 ttccttaaac tcttgatttt cattgactct aactgtattg accaaagcat caatcaaacg
     5341 agaatctgta tttggcattg gtggacgatg gtagtttacc ccaaattcct gacataagtc
     5401 ataacattcc acatcattgt caaacaagac ctcaatatgc tcactgatga aactaatagg
     5461 tacaaaaata taatggtctg gatgttctgt ctgttctctg agatactcca aaacatctgg
     5521 tttaatccat ggaataccaa tatcactctc actttgccaa gtgttggtat attgttctga
     5581 actcaagccc agtttttcag cgactagctt gctattttcg aaaatttggt ctatataagg
     5641 atcaccaaag tccaaggcaa aaataggcac actatgggct gaaaagatga ctttaaagct
     5701 gtcttgcttt acttcttctt ttaaaatctt agcaatttca tctgcccaat agtttaagag
     5761 cgcttcttcc tgataccagt ccttaatgac caaaaattga atttgtttgc tttctaaaaa
     5821 tttttcatag cccatgacag agtagaaaga ataatggggc tccaaaatca agcaaataca
     5881 ctgttcaatt ccgtctgctt ccatttgacc gatcacatct ggaataaagg gactggaaaa
     5941 cttattagca aagtagacac tgtattcact ccccagccta gcttctacca aggcaacctc
     6001 ttgacgggta attttttgca gaggcgtgcc tccaattcgt acataattat cataaagagt
     6061 ctgaatctcg tggtcttggg gtctcacccc acgacgaata tttgtgaaaa aatcagctac
     6121 accttcaaag ataatctctt ctggtgaacc gaaggtcatc attaaaattg cttttttcat
     6181 gactgcttcc ttgtgatgtt tttatcaagt ttattttatc attaattcat cgataagtaa
     6241 acgctagcat agggacattc tcaatcttct gtcttttgtt tttctcttct ttctatgata
     6301 caatggaaaa aataaattca aaaggagttt ttttatgact tatccaaatc tcttggaccg
     6361 cttcttaacc tatgttaagg tcaacacgcg ctctgatgaa cactctacta ctactccaag
     6421 tacacagagt caggttgact tcgcaacaaa tgtcctaatt cctgaaatga aacgtgttgg
     6481 actgcaaaat gtttactatc taccgaatgg ttttgctatt ggaaccttgc cagccaacga
     6541 tccgtcttta acacgtaaga ttggttttat atcgcacatg gatactgctg attttaatgc
     6601 tgaaggagtc aatccacagg taattgaaaa ctacgatggt ggtgtgattg aactagggaa
     6661 ttctggtttc aaactcgatc cagctgactt caagagtctt gaaaaatatc caggacaaac
     6721 gctcatcaca acagatggaa caaccttgct aggtgctgat gacaagtcag gaattgctga
     6781 aattatgaca gccattgaat atctaactgc tcatcctgaa attaagcact gtgagattcg
     6841 tgttggtttt ggtccagatg aagaaatcgg tgttggtgcc aataaatttg atgcagaaga
     6901 ttttgatgtg gattttgcct acactgttga tggtggtcca ctaggtgaac ttcagtacga
     6961 gactttctca gccgctggtg ctgaattgca tttccaaggt cgtaatgtcc accctggtac
     7021 tgccaaaggg cagatggtca atgcccttca gctagcaatt gattttcata atcaacttcc
     7081 agaaaatgac cgacctgagt taactgaagg ttaccaaggt ttttaccatc taatggatgt
     7141 gacaggtagt gttgaggagg cgcgtgcaag ctacatcatt cgtgattttg aaaaagatgc
     7201 ctttgaagcg cgtaaagcat ccatgcaatc tatcgctgat aagatgaatg aagaacttgg
     7261 gagcgaccgt gtcactctca acttgacaga ccagtactac aatatgaaag aagtcattga
     7321 aaaagatatg actccaatta ccattgctaa agccgttatg gaagatctag gtatcacgcc
     7381 tattatcgaa ccaatccggg gtggaacaga cggctctaag atttccttta tgggaatccc
     7441 aactccgaat atctttgcag gtggcgaaaa tatgcacgga cgttttgaat acgttagcct
     7501 tcagactatg gaacgtgcag ttgataccat cattggcatc gtagcttata aaggctaaaa
     7561 agacgaggta gctcagctac ttcgcctttc tttttattct actggttttt cttgatttcc
     7621 agtagttgta gaagattctg ttgtttcatt ttctgaagtt gattcagcag gtttagaatc
     7681 tcttgtattg cttggtttgt tttcgtcgct agcagtttca atgttagatt ctgcagttgc
     7741 gtttggttgg ttctcagcac tggtgttatc accatttgct tcagcatttc ttgctggact
     7801 tgtttcttca cttgcgctag cttttgactg gatttgatga ttcaaaacta gaatagcttt
     7861 tgtcgattca agtaaagctg ttttgtcttt actcttagca gaaagttgat ctaataatgc
     7921 atccacctta tcaaagtccg catcagatcc attattactt tctaaataag agtgaagcga
     7981 catgagaata tcgtagagtt tttgatagag tacaagtgtc tgaggatctt gctcagcatt
     8041 ttccttttct tgttgaaggg cgctagcgat acgagtcaag acatctttta cctgactgtt
     8101 tacttcatcc aagtctgcat cagccttgtt tgtggcagct tttagatttt ctacttcttc
     8161 tgccaaggat tgtctgattc cttcttcatg gatttgttcc aagagttgat ttgccttgct
     8221 caaaagactt tctacttctt ccttgctatc tgtcgcagat tattggttgc tatctaccat
     8281 gtactcctaa aacaggagag ttataatcca agattacaag gccttacaga aataagaaat
     8341 ccagataaga caatgttcgt ccaagacgct attcgcttcg cacagcagca cggattcaat
     8401 atgctttaat tttaaagttt aggtgtcaag acctcttttt agtgtgccca aaatttagag
     8461 aagtaatcaa tcaactaact tttatttttt tcaaactttc agtaaactga cctaaagcta
     8521 actcaatctg tctttgtaga tgcttctgct atcagctaga agttgatcta cttttgccaa
     8581 gactgccttc tcatcaaaag ttccaggttg atagttggat tgcagggatg gaatcttgtt
     8641 tttcaaagcc gcttcatatc ccttagtttg aaccttgatg tagtgattgt ggtcgccatg
     8701 aggaatcaca aaaccttctg aatcttcact tataattcga ttggcatcaa aaccatgacc
     8761 atcttcttcc tcatgatgga catgtagtga cggattactt aatacagaac tagaagaact
     8821 tcctacctct tccgtgttag agtgtgatgg gggattgtta agagatgact taggaatata
     8881 gtgatagtga tccccatgtc ttactatata agcatcacct gtatctctga caatatcatt
     8941 agggttaaag acatatgtgg ctgctaattc acctgccgac aagtcactct caggaatgaa
     9001 atgatagtga ccaccatgtg gtactatagt agattgaaat agaatatgag caaattgata
     9061 aggggatttt aaagtaattt ctaacaatga tttagaaact atgatgtgct attctaaatt
     9121 caactcacta tatataacca tcatcggtag tataacgtcc ctgtaatttt gctacagata
     9181 cttctgcact agctccttta tcgtctttac catgttcttg tttttggcga ttgatttcat
     9241 cttttgttcg tacattttct gcatgagctt gatctttaag gtaaacataa tactttccat
     9301 ctaccttaat aatatatcct cccttaacct aactgacgat atcttgatct ttcggctgat
     9361 agttgggggc tttcattaat agctcttcac taaagagcgc atcaaaagga actttaccat
     9421 tatagtagtg ataatgatcg ccatgagaag ttacataacc ttgatctgta atcttaataa
     9481 caatttgttt tgcttgaatt ccttcttttt gactaaccta gtctggagtc aaattttcag
     9541 tcttcttagt gtctttatta ctgtttacat atgaaacacg atttttatct gtattggcct
     9601 gttagctatg ttggttcaga gcataaacac acagacttaa ggaaaggata acaac
//
LOCUS       DAVHEW010000083         9395 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVHEW010000083 DAVHEW010000000
VERSION     DAVHEW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.083"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..1070
                     /locus_tag="VVL53_001708"
                     /pseudo
     CDS             1..1070
                     /locus_tag="VVL53_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1195..1398)
                     /locus_tag="VVL53_001709"
     CDS             complement(1195..1398)
                     /locus_tag="VVL53_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183849.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(1391..2473)
                     /locus_tag="VVL53_001710"
     CDS             complement(1391..2473)
                     /locus_tag="VVL53_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU4183850.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFNLFTSTSKDLLYFD"
     gene            complement(2599..3381)
                     /locus_tag="VVL53_001711"
     CDS             complement(2599..3381)
                     /locus_tag="VVL53_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU4183851.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3381..4724)
                     /locus_tag="VVL53_001712"
     CDS             complement(3381..4724)
                     /locus_tag="VVL53_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU4183852.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4823..6139)
                     /locus_tag="VVL53_001713"
     CDS             complement(4823..6139)
                     /locus_tag="VVL53_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU4183853.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6332..7558
                     /locus_tag="VVL53_001714"
     CDS             6332..7558
                     /locus_tag="VVL53_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU4183854.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKALT"
     gene            7757..9331
                     /locus_tag="VVL53_001715"
     CDS             7757..9331
                     /locus_tag="VVL53_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4183855.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2758 a         1813 c         2068 g         2756 t
ORIGIN      
        1 ataaagtctg acatgaaggc tggactaaag atagagtttc tcttgaatac ggtctcccaa
       61 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
      121 agaaaacaag agggagagta cctgagagcg gagaatgcca tcctaaaaaa gttaagagaa
      181 ctccgattga aggaggaaaa agagaaagaa gaaagacaga aattgttcaa gaattaatga
      241 ctgagttttc gttagatctt cttctagaag ccattaaact agctcgttgg acctactact
      301 atcacttgaa acagctagac aaaacagata aagaccaaga gcttaaaact gaaattcaat
      361 ccatctttat cgaacacaag ggaaattatg cttatcgccg ggttcattta gaactaagaa
      421 atcgtggtta tctggtaaat cataaaagag ttcaaggctt gatgaaagta ctcaatttac
      481 aagctaaaat gcgaaagaaa cgaaaatatt cttctcataa aggagacgtt ggtaagaagg
      541 cagagaatct cattcaagcc caatttgaag gctctaaaac aatggaaaag tgctacacag
      601 atgtgactga atttgccatt ccagcaagta ctcaaaagct ttacttatca ccagttttag
      661 atggctttaa cagcgaaatt attgctttta atctttcttg ttcgcctaat ttagaacaag
      721 tacaaaccat gttggaacag gcattcacag agaagcacta cgagaatacg attctccata
      781 gtgaccaagg ctggcaatac caacacgatt cttatcatcg gttcctagag aataagggaa
      841 ttcaagcatc tatgtcacgc aagggaaaca gccaagacaa cggtatgatg gaatctttct
      901 ttggcatttt aaaatccgaa atgttttatg gctatgagaa aacatttaaa tcacttaacc
      961 aattggaaca agtcattata gactatattg attactacaa caacaaacga attaaggtaa
     1021 aactaaaagg acttagtcct gtgcagtaca gaactaaatc cttcggataa attaattgtc
     1081 taactgtttg gggtcagtac acaatacctg atactatgcg ttttattgtg gggagatttt
     1141 cctcattttc tagtgaaatt gaactttttc taacatcttt tgatagatga cttcttaatc
     1201 aattatcatt tccattgttg tttcttgggg tttcatccct tgtctttgat aaaagcggag
     1261 agcaccctcg ttatcattcc agacacttag ggttagatta ttgcagccaa tttctttagc
     1321 ataatagcag aaaaatgaca ataaaatagc ccctagatat tttgaaagga aagcataggg
     1381 gctatatagt ttaatcaaaa taaagcaaat ctttgctggt gcttgtaaag aggttgaagc
     1441 catccttggt aacaacaccg cagtcttcaa tacgaacacc gactttacca gggatataga
     1501 tacctggttc aacagagaag cacatgcctt cttcgatgac catgtcgttt ccttccatga
     1561 tagatgggaa ttcatggaca tccataccga taccatgccc gagacggtgg ttgaagtact
     1621 caccataacc agctttttcg atgacctcac gggcagcgcg gtccacttca tgagcagtca
     1681 cacctggctt gataaagtca agagcagctt gttgggcttc aagagtcaag ttgtaaatat
     1741 ctttcttgaa ttggtctggt ttgccgacag cgactgtacg agtcatatct gacgcatagc
     1801 cattgaccag aacacccagg tcaaagagga gaagagcatc attttcaacc ttattagctg
     1861 ctggaatgcc gtgtggattc gcagcattat caccagtcaa gaccatggta tcaaagctca
     1921 tttcataacc ttcacgtttc atggcaaagt cgatttgtgc gatgatatct gtctcagtct
     1981 tatcaagaga aatattgtca aaaccaacat gaacagcctt gtcagcataa agacctgcaa
     2041 ccatcatttt ttgcacttca tcagctgatt tgatgaggcg catgcgttgg atacgaggag
     2101 tgaggttgtc aaactcagca gtctcaaaaa ctgttttcaa accatggtat ttggtcaaga
     2161 tgagattgtc aaactcaaca gcgacacgtt tgaagtcaag ttgtggaaga gcatgtttga
     2221 ttttttgcca tggattttca gaatcgacat agcccactac tgggaaggaa acggtgctac
     2281 ttgcacgttc tacttcaaga gctgggacaa agaggagagg ttcctgatct gctaggacaa
     2341 agaggaacat ttggcgttca tggggatcac tgtaaaaacc agtgaggtaa ttgattgtga
     2401 cggggtcaga tacgacagcg acgtctagtt tttctgattc aagatatgtt aggatttgtt
     2461 gtaatttaga catatgctac cttctttcta cccctctatt ttggcaaaaa atgagagaaa
     2521 atgcaaggga gaagggtaaa agaaaagaca aaaaactccg acaagataac ggagttcttt
     2581 gatatcattt tcctttgttt aagaaaagtc agccttgctt ttgacgtcac tgtactcttc
     2641 agcgatttct tggctgagaa tgtcctcata ggcagggagt tggatgtcct gaccatattt
     2701 cttcttgagg gcagtagtaa ctaggcgata agccagattg gcattacgag agaggatagg
     2761 cccgtggaag taggaaccaa agacattctt ataatgaacc ccttcaccga ccttttcttc
     2821 gttgtttcca tttccataga caacctgtcc cagcggtttt tggtcatcag agaggaaggt
     2881 acgaccttgg tgattttcaa atccatagta ggtttcatcg aaatcttcat tgtgaatctt
     2941 gatgtcaccg ataaaacggt tattggtctg gttgagcgtg tagtgtccca tgacccctag
     3001 cccttcgata cgttttcctg aagcttcaac ataatattga cccaatagtt ggaaaccacc
     3061 gcagatagcc agaactacac cgtcgttttg gatgtagttg tcaatgctct cttttttagc
     3121 aggtaggtcg tctgcaatga tactttgttc aaagtcttga ccaccaccga aaaaggcgat
     3181 gtcgtagtga ttttcatcaa agtcatcatg gagagaaacg atgtcaacgg tcacatgggc
     3241 tcccagtttt tcagccacat acttgagcat gaggatgttt ccattgtccc cgtaggtatt
     3301 catgagattt ccgtagaggt gggcaatgtt gagctgatag gggtaattgc catcttttga
     3361 ggaaagtgaa gtataaacca ttagttcatc tcctttctaa caatctgacg actagccagc
     3421 agttcacgaa attccagcat ggcagtataa gttgccagaa tataggcatg cttgcagtct
     3481 tgattctcaa tggtcttgag aacttgctcc agattactcg tttcagtgat tttctcagct
     3541 ggatagccag tcactcggag gcgacgagcg atttcagaat gacgaacacc gccagcgttg
     3601 atttcaggaa tgtccatgtc agtgatttgt tcaaagtctg catcccagat ccagctagtg
     3661 tcaattccat ctgcatagtt ggcattaagg aggacagata ggctaaatgg ataaggtgct
     3721 agtttgatca tttcgatagc ttgggttgca ccgactggat ttttaatcaa gacaagggta
     3781 cattccttgt caccgatatg aaaggtttct tggcgtccaa agacagcacg gctcttgtca
     3841 aatccctgtt tgatgagttg cgaatcggca cctaggaaac gggcgatggc cacagcagct
     3901 agggcgttat agatattata gagcccgccg atttggatac cgtattcttg gccgtctatg
     3961 acaaagcgag agcgattgtt ggtcaactca accagttttg tcaaacgata gtcgagatca
     4021 ggacgtttac atccacaacc ttcacagata taggcaccca agtttgcata ggtattatgc
     4081 tcatatttga ggatgccttg gcagtcagga cagagaatcc cttcggtatt gtagtgagcc
     4141 agttgggctg gtcccttttc caagtcaaaa ccaaaatact ctatagggtt tggaatagtt
     4201 ggcttgtaga aaagtggact gtctccgtta aggagaacag tagcagttgg aactttccga
     4261 atggcatcca atatcatgtt ataggtagta tagatttcac cgaaacggtc catctggtca
     4321 cggaagatat tagtaatgac aaaaagacta ggctggatat agtcacagat acgagataga
     4381 ctggcttcgt caatttcgag gacggcaata tttttcccag ttttagaaga tttggctgtt
     4441 aggaaggttg ttgcaatccc tgtaatcatg ttggcaccgc ttgggttggt tagaacttga
     4501 ccataaacct cttttaaaat gccgacagtg agggcagttg tcagggtttt tccatttgtt
     4561 ccagtgacaa cgacaatctc gtagttctta gctaggtttt gtaaaatatc tttatcaaat
     4621 tgaagggcga ctttccctgg gagcgtactt ccacgtccaa gacggcttaa aacgaagtgg
     4681 gaagaacgcc cagcaagaag gcccaaagta gtttttaagt tcatgtttct attatatcat
     4741 aaaagaggag aaagacagat ttgagattcc gcagaagtaa aaacagccca aagagggctg
     4801 ttcgatacga ttaaatctca acttaaatcg caaacaagtc attcaagttc tcagccaagg
     4861 ttgggtgagt gaagatttgt tttgtgaagt aagtgtaagg aatcttgttg tccatagcaa
     4921 cagtgatgat gttgatgatt tcttgagaac cttctgagaa gatgcttgct ccaagaattt
     4981 cttttgtttc agtattgaca acagctttga aggcaccgcg aaggtctcca tttacgtgac
     5041 cacgaggcat tgctgcaacg gggatttcct taacagcgta tggaagtttc aaatcagctg
     5101 cttggctttc agtcaaacca acttgtgaaa gtgcaggtgt gatgaacata gtatttggca
     5161 cattgagacg gtcttcaagt gtatagctgc catctccagc aaggtagctg taaacaacac
     5221 ggaagtcatc aagtgaaatg taagtaaatt gaaggccacc gttgacatct ccaactgcaa
     5281 agacaccagg aacgtttgtt tgacagtgtt tgtctacttt aatagcacca cgttcagtta
     5341 gttcaatatc tgtattttca agttgaagtg gttctacatt tggtttgcgt ccagttgcgt
     5401 agagaagggc gtcgaaacgg taagtttcgt cttcagttac gacaagcact tggtcaccat
     5461 cgtttttgat ttcagtagta tggatatttt gaagcaattc aatgccatct tcttccatgt
     5521 attgtttagc aagagctgcg atggaaggtt ctgcacgagg taggaatgta tccaaggcat
     5581 ctaggactgt gaccttgctt ccaagtttgt tgtaaaggcc ggcaaattca agaccgatat
     5641 ttccgccacc aaggattcca agtttttcag gtaatttgtc caagctttgg atacctgttg
     5701 agtcaaagat gtttttgctt gtagcaagtc cagggattgg caagacgttt gaaacagcac
     5761 cagtgttgat gacgattgtt tcagcagtca gttctttctt ttcatcacca gcttggattt
     5821 cgatgacttt atttgaaagg aagtgagctt ccgcatcaaa gatatctacg cctgtaccag
     5881 caacagtcgc atagttttta ccgttgaggc gaccagtgat cgtgttttta gtagcaatga
     5941 cttcttcaaa agacaagtcc ttttcagcag caactagcaa ggttttagtt gggatacaac
     6001 caatgttgat acaagttcca ccgtacatag ccttgctgcg ttcaacgagg gcaacttttt
     6061 tgccagctga agccaattta cctgctagtg ttttaccagc tttaccaaat ccaataacaa
     6121 ttaaatcgta tgttaacatt tatcttcctc ctattcgaat ttcattctag cacaagaaaa
     6181 aaacttttgc acaattctgc tacggaaaag acagaaaaag ataaaaacgc tttccagaca
     6241 aaaaactagt aattttttga caagcttgat aaactaaaat cctatctatt tttatataag
     6301 atagaataaa tgttcgaaaa taggaggttt tatgaaatcg aatcgttata ttattgcctt
     6361 tgctggggtc attttacact taatgctggg ttcgacttat gcttggagtg tttatcgtaa
     6421 ccctattatt gaaaaaacgg gatgggatca ggcttctgtt gccttcgcct ttagtctagc
     6481 aatcttttgt ttgggcttat cggctgcatt tatggggcgt ttggtagaaa aatttggtcc
     6541 gaaagtcatg ggaagtctat ctgcttttct atacgcaggt ggaaatatct taacaggatt
     6601 tgcaatagac cgtcaggagc tgtggttgtt gtatctcgct tatggcattt taggtgggct
     6661 tggtttggga gcaggctata ttacccctgt gtcgacgatt ataaaatggt ttcctgataa
     6721 acgtggtctc gcaacaggtt tagcgattat ggggtttggt tttgcttctt tattgactag
     6781 tcccatagcg caacacctca tcgcaggggt agggcttgta gaaacttttt atattttagg
     6841 agcaagttac tttattatca tgctcctagc ttcacaattc attaagcgtc caaatgagca
     6901 agagcttgca attttatctt cttcagggaa agaaaaaaca gcctctttga cgcaaggaat
     6961 ggctgcaaat caggctctaa aaagcaatcg gttttatatg ctttggatta ttttctttat
     7021 caacatagct tgtggtttag gcttaatttc agcggcatcg ccaatggcac aggagatggc
     7081 tggcttgtct acaagtcatg cagcagtaat ggtgggtgtt ttggggattt tcaatggatt
     7141 tggtcgcttg ctctgggcga gtttgtctga ctatatcggt cgccctctaa cctttagtat
     7201 attactgctt gtcaatcttt tcttttctct ctcactttgg ctctttacag attccgtttt
     7261 atttgtagtt gctatgtcta ttttgatgac ttgctatgga gctggttttt ctttgattcc
     7321 agcttatctc agtgatattt ttggaaccaa ggaattggcc gctctgcatg gttatatttt
     7381 aacagcttgg gcaatggctg gtttagcggg acctatttta ttagcagaga cttataaaat
     7441 ggctcattcg tacacacaaa ccttgttcgt ttttctcatt ttatacagta tcgccttggc
     7501 tttgtcttat tatctaggtc gttcaatcaa aaaagaaagt caaaaagcgc ttacatgatt
     7561 ttctgtagaa atggggctat cttttgctct ggtcttgtgt tatactagat aggttgcaaa
     7621 gaaaacagta cttttctttt gtggaaaaga agcaacacga ttttatatct agttatatga
     7681 aaatacgtca taaaaagaaa agtataaact aagccctata gggtggcttc gcaccacctt
     7741 tagaaagaag aataacgtga aatttaatga attaaacttg tctgctgatt tgctagcaga
     7801 aattgaaaaa gctggttttg tagaagctag tcctatccaa gaacaaacta ttcctttggc
     7861 ccttgaaggc aaggatgtta tcggtcaagc tcagactggt acaggaaaaa ctgcagcctt
     7921 tggcttgcct acccttgaaa aaatccgtac agaagaagcg actatccaag ccttggtcat
     7981 cgctccaact cgtgaactag ctgtccaaag tcaagaagaa ctcttccgct ttggtcgtag
     8041 taagggagtc aaagtccgtt cagtatatgg cggatcaagc attgaaaaac aaattaaggc
     8101 tcttaaatct ggtgcccata ttgtggtggg aactccaggt cgcctcttgg acttgattaa
     8161 acgcaaggcc ttgaaattac aagacattga aacccttatc cttgacgaag cggatgaaat
     8221 gcttaacatg ggcttccttg aagacatcga agccattatt tcccgtgtac ctgagaaccg
     8281 tcaaactttg cttttctcag caactatgcc agatgccatc aaacgtatcg gtgttcagtt
     8341 tatgaaagcc cctgaacatg tcaagattgc ggctaaggaa ttgacaacag aattggttga
     8401 ccagtactat atccgtgtta aggaacaaga aaaatttgac accatgactc gtctcatgga
     8461 tgtggcacaa ccagaactcg ctattgtatt tggtcgtacc aaacgccgtg tggatgaatt
     8521 gactcgtggt ttgaaaattc gtggcttccg tgcagaagga attcatggcg acctagacca
     8581 aaacaaacgt cttcgtgtcc ttcgtgactt taaaaatggc aatcttgatg ttttggttgc
     8641 gacagacgtt gcagcgcgtg gtttggatat ttcaggtgtg acccatgtct acaactacga
     8701 tattccacaa gatcctgaga gttatgttca ccgtatcggt cgtacaggtc gtgctggtaa
     8761 gtcaggtcaa tctattactt ttgttgctcc aaacgaaatg ggttaccttc aaatcattga
     8821 aaacttgact aagaaacgca tgaaaggtct caaacctgca agtgtagaag aatccttcca
     8881 atcaaaaaaa caggtagctc tcaagaaaat cgaacgtgat tttgcagatg aaaccattcg
     8941 tgccaacttt gagaaatttg gtaaggatgc tcgcaaattg gctgctgagt ttactccaga
     9001 agaattggca atgtatatct tgagtctgac agtccaagac ccagatagcc ttccagaagt
     9061 ggagattgca cgtgaaaaac cactaccgtt taaaccatca ggtaatggtt tcggtggtaa
     9121 agctaaggga ggtcgtggag gccgtcgtgg ggacgaccgt cgagagcgtg atcgccgtgg
     9181 caatggtcgc cgtgatgagt tcaaaaaagg aagtcgtggc aacgatcgtt ttgataagga
     9241 aaaacgttac cgtaaggata ataaaaaacc acgcaatact ttaagcgaaa agcaaacagg
     9301 ctttgttatt cgtaacaaag gcgataaata agaaaaagcg gttcaaaatg aatcgctttt
     9361 ttatataagg agaccgaatg tgaaaagaca aataa
//
LOCUS       DAVHEW010000084         9219 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVHEW010000084 DAVHEW010000000
VERSION     DAVHEW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.084"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(552..1400)
                     /gene="dprA"
                     /locus_tag="VVL53_001716"
     CDS             complement(552..1400)
                     /gene="dprA"
                     /locus_tag="VVL53_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU4183856.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(1497..2186)
                     /locus_tag="VVL53_001717"
     CDS             complement(1497..2186)
                     /locus_tag="VVL53_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU4183857.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2198..3076)
                     /locus_tag="VVL53_001718"
     CDS             complement(2198..3076)
                     /locus_tag="VVL53_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4183858.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(3066..3935)
                     /gene="licA"
                     /locus_tag="VVL53_001719"
     CDS             complement(3066..3935)
                     /gene="licA"
                     /locus_tag="VVL53_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU4183859.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3952..4974)
                     /locus_tag="VVL53_001720"
     CDS             complement(3952..4974)
                     /locus_tag="VVL53_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU4183860.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4979..5686)
                     /locus_tag="VVL53_001721"
     CDS             complement(4979..5686)
                     /locus_tag="VVL53_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU4183861.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            6022..7509
                     /gene="tacF"
                     /locus_tag="VVL53_001722"
     CDS             6022..7509
                     /gene="tacF"
                     /locus_tag="VVL53_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU4183862.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWINEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7519..8322
                     /locus_tag="VVL53_001723"
     CDS             7519..8322
                     /locus_tag="VVL53_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU4183863.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     INLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8324..9133
                     /locus_tag="VVL53_001724"
     CDS             8324..9133
                     /locus_tag="VVL53_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU4183864.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKS"
BASE COUNT         2896 a         1859 c         1549 g         2915 t
ORIGIN      
        1 taatctctac ttttgaagaa tttatttttc cttactaaca agatttgaga tggtaggaat
       61 cattgaacat aacctagcca acatcaatca caatcatttc tcctttctca attacactaa
      121 attatagtgt attgaatcta taacagtgca ccttggctgc taaaatattt ctataaatta
      181 atttgacttt cctgatagag ttgttcacat cttatttcaa ttcactatac tttcccttat
      241 actcaacgaa aatcaaagcg caaactaaga agctagccac aggttgctca aagcactgct
      301 ttgaggttgt agataagact gacgaactca gctcaaaata ctgttttgag gttgcagatg
      361 gaagctgacg tagtttgaag agattttcga agagtattat atgagaaaaa tcctctactc
      421 tactaatcca cttagaaaaa acgcatatta gcaagtgtta aaaacttgct aatatgcgtt
      481 ttattatgga aatgttgggt tcattttctc ttaaaattga ttttttctta gaattctagc
      541 ttaggtcatt tttaaaattc aaattccgca agaacatctt gcccactggt gagcaatttt
      601 gctccttctt gaatcaaatg atggcaaccg tctgatagtc catctaaaat gctagcagga
      661 atagcaaaga catcgcgtcc ttcttccatt gctcgctcac acgtaatgag actacctgaa
      721 cgcatcttag cctctgctac aatcacacca cgacaaagtc cagcaatgat gcgattacgg
      781 gcaggaaaat gaaatttcag aggttgttcg ccaggtccat attcacttag aaccagatgg
      841 tcattgccga tgtagtcttg caagcgttta ttggctttag gataaaacac atccagtcct
      901 gttccaatca ctgcaatggt ttttccgcca ttctgaagag ctgccatatg agctgctgtg
      961 tcaatgccct tggccagacc actgacaata accagttcat tttccaagcc ttgaatgact
     1021 ttttcaactg acttagctcc ctgtttgcta caagcacgac tgcccacgac cgctaccttc
     1081 gggaatttca ggaggtcaag atttcccttg taaaataaaa gtacaggcgc atcatatatt
     1141 tcactcaaat cccaaggata acagtcatct aaaatagaga aagatggaaa tttttgaaac
     1201 tctttcgaca aatgcgcatc gtctatctga aaataacgtt ccataaaaac ggctggatta
     1261 cggcaacctg atatatctgc aatatcaccc aacaaaagct cctgatcaac attttcaccg
     1321 tattctagca ctttcaaaat ctgttgattg gtcaaacctg atttttttaa cttatagatt
     1381 tcatagtttg tgattttcat aaataactcc atttcttttt ctactcatct atctattcgt
     1441 aaaagttcgt aaaaaatcaa aaaacatccg actattttgt cagatgttgg aagcttttaa
     1501 ttttcgtttt taagaatttc ttctaattta cgatagtctt ggacactatc gatctcataa
     1561 atgctattgc cttctaattc ttcaacatag acatctagct ctttgatatt atccttaacc
     1621 atattgtccc aatagagatc aacaaattca ccacttgcat aagccttgtc gataaagctg
     1681 acaatctttt ctgcagttgg agcatcccag aaggatacac cactaaggat gcgacctgcc
     1741 ttgctatcaa caataatgtc ttgaaccttg tagtcatctc catagaccaa gaaccattcg
     1801 ttggtacaat cttcacgata aacactaaaa taagtcgaac gtgtcaaatc attgcggaac
     1861 atatttttaa agagataatt gtcagcatca ataacatagc tgttggccaa ttcttctttt
     1921 acaagataga gagagtaaaa gttattgtag tcagcgtatt tatcattgaa aacgaggcga
     1981 acaccgtatt tttcttttaa gtaatcgaat tgttctttaa gataaccaac aatgatgatg
     2041 atgtcattga ttcctttttc tttgagaaac tcaatttggt attcaatcaa aggtttttga
     2101 ttaacctgaa ccaaggcttt aggggtattt tcagtcatag gacgcaagcg agttcccaat
     2161 cccgctgcta agataatggc tttcacacga atctccttta ttctttaata ataatataaa
     2221 ctccagcaat gacgacaagt gacgtcataa tggtcagcat atctagcggt gcacccaaaa
     2281 aaacaactgc aaacaagacc gtccatacta catagctcac gttcaagcct gtagccttgg
     2341 ctggttgcaa gcgattgata gcgatataat aagccaagta ggaaatcata tcaaaggctg
     2401 caaaaacaat catgagacct agcaattgtc cattggctac tgcagtaaat gactgatgag
     2461 agaagagcac aatcacaaga taggacaaga acgaagtcac ttgacggatt aagagggctt
     2521 cgatttcact cagttcactt tccatagcaa aggagctaag aacactctca cttccccatg
     2581 caatagcaca aaccaaagca cagagaatcc caatgtagaa ggaattaacc tgttcaacct
     2641 tataggtctg ggcaataatc cctccaataa tcaagacaat cccaaataca gtatttttcg
     2701 aaatcttgtg cttcaaaaag aagaaagcca atagaactga aatcgcaggg taaatagccg
     2761 atacagatga agctaaagaa cttccgatat acttaactgc ataaagattg gcctgcatac
     2821 cgatagggcc tgctagcaag gctccgatga taacactgac attgcgaata tttaagaaaa
     2881 ttgagaggcg aactttccct tcttttacca agagaaaagc tagtaagata aagatgctca
     2941 aaaaatcatg agctgcagcc accacaaagg gtgacaaatc tgtaaaaatc gaaaagatat
     3001 aagcactaac cgttagaccc aagccccaga aaatacctga gagaaggcca aaaggaactc
     3061 cgtttttact tttcatctga acctccataa gaagccaaac ctttaacagc tctttggtaa
     3121 cgattcacac catagtcacc aaaatctgca ccttgctctt ccttatagac agtccataga
     3181 ctccaaatag tatcttgtaa aattttataa atagcaatct tttcatgaga aaccggtgtt
     3241 tggtcactct catagtgaga taagaaagtt tcttcctctt gggaagtgaa ttcagactct
     3301 aaaaagaggg cagccaaatc ccacattgga tcattcattg atgaatattc ccagtcaatc
     3361 aaataaagtc gtccttgagg tgattcgata aagttttcag gcaccaaatc gatatgacaa
     3421 gattttctgt caacacctaa gtcagccagt cttttctcta aggagaagac tgcatttcta
     3481 acagattcat agttggcata aggaatttgt tcttcaatca aggattcgta ttttttgatt
     3541 tcttcaaaag gagcaaattc tcctcttaat tccttagcag acgtatgaat agtttgtaat
     3601 attggagcaa ttttgtcgaa cttggtcttg attgacgttg aatcaagcgt aatcgcagat
     3661 tcgatatact catttacttt gataccagct tcaatatcaa aaagataatt ttttacatct
     3721 aagcctaaat cctttagtag ttcaagattg tacttttcat cttgtcgatt gataagcttt
     3781 tctgtccctt taccaaagaa tttaacaatg tattgcttat ttgttgtttt ggccaaatag
     3841 ttttgattgg tcattccacc cagttgttca acactgagga cttcctcttc ttgactaagt
     3901 aaggaagaaa ttttttcttt aatgattttc tccacaatta acctccagta cttatacttc
     3961 ccacttaaac actgttttaa aggctgtgtt taaatcggtt gcaaagacac gatgaatatc
     4021 tttaatttct cttacaggtt cttctagata aaggatattt ttaagacgat tggcaaattt
     4081 cttgacttcc atcatttgga tagcattttt aaaatcaatg cgaccagaac gagatgaccc
     4141 aaccaaaatc aagccctttt ctaaggcatc gcgagtattg agattgactt tatattcgct
     4201 aactcccatc atgagaatcg ttccctgagg acgaatgtag cgaatcaagt cattaatagc
     4261 tggtccagta ccatcaccac cacaacattc aaaagcatgg tcaaaggcca actcttcagg
     4321 aatattatcc gtaatatagc attctttggc aaatgagaag agttccaact tttcccaatg
     4381 acgaccaata accacaatct ctgattctgg caaagtatag ttgataatat tggcaaccac
     4441 aaaagctaaa cttccatctc caataacggc gatccgctcc cgcttgctat gagcaagagt
     4501 caatagacga ttcatagcgt gcatgcccac actgacaaac tctgtaatgg ctgcaaccgt
     4561 atcttcaata gcatcataag ccaccacacg atctttaggg agagaaacaa actctctcat
     4621 aaagccatca aatccactag acaagaaatg ggtccctgtc atgtagtttt catagaattc
     4681 ttcatcactc tgcataggag actgattggg aatcatgaca actttttgac caacctcgta
     4741 ggttccggtc gggtcagaaa tgacggttcc acatgactcg tgaatcattg ccattggaag
     4801 ctttttattc aaaatcttgg gatcacgttt tccctgatag taacgctgat ccgcatgaca
     4861 gacagccatg tagttgggac ggataaggat atgattctct tggtcaatag cctcttcctg
     4921 atatttgaca ttgataaact taggcttagt tagttgataa atttgattaa tcattttact
     4981 agtctttctc aatcatactt tttgcaatct tcaaatctgt tacggttgta atcttcagat
     5041 ttgagtattc acctttggcc aaggccacat cttttccttt gatcacaaag attttacatg
     5101 catctgtcaa gatttccttc tcttcatcag aaagagatcc ataaaggtcc atgaagtcct
     5161 tgcaacggaa tgtttgaggt gtttgtccct gatagaggtg agcacgattt ggaatacctg
     5221 taatgaattg cccattggta ctttcaacga tagtatccac tgcttctacc actgtgtcca
     5281 ctgcgtcatg attttgggca agttggatat tgtcctgaat catgcgaagt gtaataaatg
     5341 gacgaacaga atcgtgggta acaacgatat cctctgaagt aagcggacga taagcatcaa
     5401 tggcttcaat gatgttctca atacttgtat tgcggtcagc accacccttt gtaatgatga
     5461 tacgttcctt ataaagagga agatatttat ctacaagatc ttctgcatga gaaacccagt
     5521 ctccatgaac accaactaca attttttcaa tacttggctc caagacaaat ttttcaattg
     5581 tatgaatcaa aataggtcga tcacctagct ctaaaaattg ttttggcaag ttactgatcc
     5641 ccatgcgtgt gccagttcca ccggcaagaa ttcctgcata aatcatctat tttctccttt
     5701 gtcttactaa aaaaacttat tctctctatt ataccacaat ctagtcctat cataaaagaa
     5761 atactggtcc tccccttcta gaataggaga attatgcagt tctattattc aaagaaacta
     5821 gtccatttct agtaatgact aggaattctg tagcaattac taataaatga ctgcgaaatt
     5881 ttagagtcct tcagaataat atactttcct taaagaaaac ttaaaattta agatttgaaa
     5941 cttcaaagtg attgcttggc ttactgccct tcatttctta ttagctaact ttatgatata
     6001 atgaaaaacg aggtaaattg aatgaaaagt ataaaattaa atgctctatc ttacatggga
     6061 attcgtgtct tgaatattat ttttcccatc ctaactggaa cctatgtcgc gcgtgtcttg
     6121 gaccgaactg actatggtta cttcaactca gtcgacacta ttttgtcatt tttcttgccc
     6181 tttgcaactt atggtgtcta tagctacggt ttaagggcta tcagtaatgt caaggataac
     6241 aaaaaagatc ttaacagaac cttttctagt cttttttatt tgtgcatcgc ttgtacgatt
     6301 ttgaccactg ctgtctatat cctagcctat cctctcttct ttactgataa tccaatcgtc
     6361 aaaaaggtct accttgttat ggggattcaa ctcattgccc agattttttc aatcgaatgg
     6421 atcaatgaag ctctggaaaa ttacagtttt ctcttttaca aaactgcctt catccgtatc
     6481 ctgatgctgg tctctatttt cttatttgtt aaaaatgaac acgatattgt tgtctataca
     6541 cttgtgatga gtttatcgac gctgattaac tacctgatta gttatttttg gattaaaaga
     6601 gacatcaaac ttgttaagat tcacctaagt gattttaaac cactctttct ccctctgaca
     6661 gccatgttag tctttgccaa tgccaatatg ctcttcactt ttttagatcg cctcttcctc
     6721 gttaaaacag ggattgatgt caacgttagt tactatacca tagctcagcg aattgtgacc
     6781 gttatagctg gggttgtaac aggtgcaatt ggagtgagtg tgcctcgtct cagttactat
     6841 ctggggaaag gagacaaaga agcctatgtt tctctggtta atagaggtag tcgaatcttt
     6901 aacttcttta tcattccact gagttttgga ctcatggttt taggaccaaa tgccatccta
     6961 ctttacggta gtgaaaaata tatcggaggc ggcatcttga cctctctctt cgcttttcgt
     7021 acgattattc tggccttaga taccattctt ggttcccaaa ttctctttac caatggctat
     7081 gaaaaacgta tcacagtcta tacagtcttt gctgggctac taaatttggg cttgaatagt
     7141 ctcctctttt tcaaccatat cgtggctcct gaatactact tactgacaac tatgttatca
     7201 gagacttctc tacttgtttt ctatatcatt ttcatccata gaaaacaact catccacttg
     7261 ggacatatct ttagctatac tgttcgatac tctctctttt cactttcctt tgtagcaatt
     7321 tatttcctga ttaatttcat gtatcctgtg gatatggtca ttaatttgcc atttttgatt
     7381 aatactggtt tgattgtctt gctatcagct atctcttata ttagtctact tgtcttcaca
     7441 aaagatagca ttttctatga atttttaaac catgtcctag ccttaaaaaa taaatttaaa
     7501 aaatcatagg agtttaaaat gaaacaacta accgttgaag atgccaaaca aattgaatta
     7561 gaaattttgg attatattga tactctctgt aaaaagcaca atatcaacta tattattaac
     7621 tacggtactc tgattggggc ggttcgacat gagggcttta tcccttggga cgacgatatt
     7681 gatctgtcca tgcctagaga agactaccaa cgatttatta acatttttca aaaggaaaaa
     7741 agcaagtata agctcctatc cttagaaact gataagaact actttaacaa ctttatcaag
     7801 ataaccgaca gtacgactaa aattattgat actcgaaata caaaaaccta tgagtctggt
     7861 atctttatcg atattttccc tatagatcgc tttgatgatc ctaaggtcat tgatacttgt
     7921 tataaactgg aaagcttcaa actgctgtct ttcagtaaac ataaaaatat tgtctataag
     7981 gatagccttt taaaagattg gatacgaaca gccttttggt tgctccttcg accggtttct
     8041 cctcgttatt ttgcaaataa aatcgagaaa gaaattcaaa aatatagtcg tgaaaatggg
     8101 cagtatatgg cttttatccc ttcaaaattt aaggaaaagg aagtcttccc aagtggtacc
     8161 tttgataaaa caatcaattt accctttgag aatttaagcc ttcctgcacc tgaaaaattt
     8221 gatactattt tgacacaatt ttatggagat tatatgaccc taccaccaga agaaaaacgc
     8281 ttctacagtc atgaatttca cgcttataaa ttggaggatt aggatgcaat atttagaaaa
     8341 aaaagaaatt aaagaaattc aactagccct gctggactat attgatgaga cttgtaagaa
     8401 acatgatatt ccttattttc tcagttatgg aaccatgctt ggagccatcc gccacaaagg
     8461 tatgattcct tgggatgatg atattgatat ttccctttat cgtgaggatt atgagcgttt
     8521 actgaagatt attgaagaag aaaatcaccc tcgctacaag gttctttcct acgatacatc
     8581 ttcttggtac ttccataatt tcgcatcgat tttggacact tctactgtta tagaagacca
     8641 tgttaagtac aagcgtcatg ataccagcct tttcatcgat gtcttcccaa ttgatcgatt
     8701 tacagacttg agcattgtcg acaagagcta taagtatgtg gctcttcgtc aactagctta
     8761 tatcaaaaaa tcacgagcag ttcacggtga tagcaaacta aaagattttc ttagattatg
     8821 tagctggtac gctctccgat ttgtcaatcc tcgctacttt tacaagaaaa ttgatcaact
     8881 agtcaaaaat gctgtaacca acactcctca atatgaagga ggagttggga tcggtaagga
     8941 agggatgaaa gaaatcttcc cagttgatac ctttaaagaa ctgattttaa ctgagtttga
     9001 gggccgtatg ttgcctgttc ccaaaaaata tgaccaattt ttaacccaga tgtatggcga
     9061 ttatatgaca ccaccatcaa aagaaatgca agaatggtat agtcatagta ttaaagctta
     9121 tcgtaaaagc tgattgaggg ggattataca aactactaag atagaggtta ttcaaaaaca
     9181 taattttagt agaaaatgaa atacatattc ccacaataa
//
LOCUS       DAVHEW010000085         8926 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVHEW010000085 DAVHEW010000000
VERSION     DAVHEW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.085"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            129..284
                     /locus_tag="VVL53_001725"
                     /pseudo
     CDS             129..284
                     /locus_tag="VVL53_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            484..3759
                     /gene="rexB"
                     /locus_tag="VVL53_001726"
     CDS             484..3759
                     /gene="rexB"
                     /locus_tag="VVL53_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU4183865.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQESQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3756..7406
                     /gene="addA"
                     /locus_tag="VVL53_001727"
     CDS             3756..7406
                     /gene="addA"
                     /locus_tag="VVL53_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU4183866.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDINHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAIELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7621..8799
                     /locus_tag="VVL53_001728"
     CDS             7621..8799
                     /locus_tag="VVL53_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183867.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSFIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSNFDERLLKWFWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2773 a         1854 c         1888 g         2411 t
ORIGIN      
        1 attatagtag attgaaataa gatacgaata gcttgattag ggaaatcaaa ttaatttcta
       61 gaaatatttt agcatccacg gtgtactatt atagtttcaa tttactatag ttcaatcatt
      121 ctatcaagaa tattgaatat ataaaggcac gtaatattaa aacttcttct gatatcgaat
      181 tagagtccga tgttgatggc gataaatcag acaatctacc agtcgacatt aagatactgg
      241 gacaacacat tgaagtgtat tccgtaccta agaaacaatc ataaaactca aagccttgtt
      301 accgaggctt ttttattatg tgattttcag tagccagact tattctatct aatacaagta
      361 aagctaagta tttaagattt agcagatgat gaagaaaaga aaggatggcc taaatatttt
      421 aacagattta cgaggctaat catggtataa tatagggtga tgaaaaattc caacgaggct
      481 gagatgaaat tactttatac tgatattcgg acttctttga cagaaattct aacaagagag
      541 gcagaagagc tagttgcagc tggcaagcgg gtcttctaca ttgcccccaa ctctctttct
      601 tttgaaaagg aacgcgccgt gctggaatac ttgtcccagc aggcttcttt ttcgattacc
      661 gtcacgcgct ttgctcaaat ggctcgctat ctggtcttga atgatttacc agctaaaact
      721 actcttgatg atatcggtct tgggttggcc ttttacaaat gccttgccga actcgatccc
      781 aaggacttgc gtgtttatgg cgctattaag caggatcctc aattgatcca gcagttaatt
      841 gagctttacc atgagatgac caaatctcag atgagttttt tggacttgga gaatttaaca
      901 gatgaggata agagggcgga tttactcttg atttttgaga aagtaacagc ctatcttaat
      961 caaggtcagt tagcccagga aagtcagttg tcccatttga ttgaggctat tgagaatgac
     1021 aaggtaagta gtgattttaa tcaaatcgcc ttggtcattg acggctttac tcgtttttct
     1081 gctgaggaag agcgggttgt ggacttactt cacggcaaag gtgttgagat tgttatcggg
     1141 gcttatgcta gtaagaaagc ctataccagt ccttttagcg agggcaatct ctaccaagcc
     1201 agcgtaaaat ttctccatca tctggcttct aaataccaaa cgcctgctca ggactgttct
     1261 caaactcatg agaagatgga tagttttgac aaggcctctc gtttgttgga gtcttcttat
     1321 gacttttcag aactcgcttt ggatgtcgat gagaaagacc gtgaaaattt acaaatctgg
     1381 tcttgtttga cgcaaaagga ggagttggag ctagtagccc gtagtattcg tcagaaatta
     1441 catgagaact cagacctgag ctacaagcat tttcgtattc tcttggggga tgtagcttct
     1501 taccagttat ctctcaaaac catttttgac cagtatcaga ttccttttta tcttggtaga
     1561 agcgaagcca tggctcatca tcccttgact cagtttgtcg agtctatttt agctttaaaa
     1621 cgttaccgtt ttcgtcagga ggatttgatt aatcttctta gaactgattt gtatactgac
     1681 ctcagtcagt ctgatattga tgcttttgag caatatatcc gctatcttgg tatcaatggc
     1741 ttgccagcct ttcagcaaac cttcaccaaa tcccaccatg gaaaatttaa tcttgagcgt
     1801 ttgaatgtcc tccgcctgag aattttagca cctcttgaaa ccctctttgc cagccgaaaa
     1861 caaaaggctg aaaaactcct acaaaaatgg agtgtctttc taaaagaagg agctgtgacc
     1921 aagcagttac aagatttgac aaccactttg gaagctgtag aacaggaaag acaagccgaa
     1981 gtttggaagg ctttctgcca tgttttagaa caatttgcga ctgtttttgc tggttcacag
     2041 gttagtctgg aagacttcct agccttgctc cattctggaa tgagtttgtc ccaataccgt
     2101 accattccag caacagtgga cactgttctg gtgcagagtt acgatttgat tgcaccattg
     2161 actgctgact ttgtctatgc tattggacta actcaggaca atttaccaaa aatttctcaa
     2221 aacaccagtc ttctgacaga tgaagaaagg caaaatctaa accaagcgac cgaagaaggc
     2281 gttcaattac tgattgccag cagtgaaaat ctcaagaaaa atcgctacac tatgctttcc
     2341 ttggtcaatt ctgctcgtaa gcagttgttc ttgtcggctc caagcctttt taacgaaagt
     2401 gaaagtaagg aatctgccta tcttcaagag ttgatccatt ttggatttag gcggagagag
     2461 aagaggatga atcacaaagg actgtctaag gaggatatgg ggtcctatca cagtcttttg
     2521 tctagtctgg ttgcctatca ccagcagggt gagatgagcg atactgagca agatttgact
     2581 tttgtcaagg ttctgtcgcg tgtcataggt aaaaaactag atctgcaagg tctggaaaat
     2641 ccagctatcc caaccagtcc aagcagcaag accttagcca aggacacctt gcaagctctc
     2701 tatcctgcca aacaggagtt ttacctgtct acgtcgggtt tgacagagtt ttatcgcaat
     2761 gaatacagtt atttcctacg ctacgtttta ggcttgcagg aggaattacg tttgcgtcct
     2821 gatgcccgta gtcacgggaa tttcttgcat cgtatctttg aacgcgcctt acagttgcct
     2881 aatgaagatt cctttgacca acgtctagaa caagctattc aagaaaccag tcaagaacgc
     2941 gaatttgaag ctatttatca agaaagtttg gaagcccagt ttaccaagga agttttgctt
     3001 gatgttgcac ggacgactgg acatattctc cgacacaatc cagccatcga aaccatcaaa
     3061 gaagaagcaa attttggtgg aaaagaccaa gcctttattc aattagacaa tggacgcagt
     3121 gtctttgtac gaggcaaggt ggaccggatt gaccgtttga aagctaatgg agcgatagga
     3181 gtagtagact acaaatccag tctgactcag ttccagtttc ctcatttctt taatgggctc
     3241 aattctcagt taccaaccta tcttgctgcc ctaaaaagag aaggggagca gaactttttc
     3301 ggcgccatgt acttggaaat ggctgaacct gtccaatctc tgatggcggt aaaaagtctg
     3361 gcaggagcag tggtagaagc cagcaaatct atgaaatacc aagggctctt cttggaaaaa
     3421 gaaagcagtt atttaggcga attttataac aaaaacaagg ctaatcaact gacagatgag
     3481 gaatttcagc tcctactgga ctacaatgcc tatctttaca agaaagctgc tgagaagatt
     3541 ttagcaggcc ggttcgccat caatccttat actgaaaatg gcagaagcat tgccccatac
     3601 gtccagcaac atcaggctat tacaggcttt gaagccaatt accatctggg ccaagcccgt
     3661 ttcctagaaa agttggacct agctgatggc aagcgtctgg tcggagaaaa actcaagcaa
     3721 gcttggtttg aaaaaataag agaggagtta aatcgatgaa gcttattccc tttttaagtg
     3781 aggaggagat tcaaaaactg caagaagcag aagcaaattc gagcaaggaa cagaagaaaa
     3841 ctgccgaaca aatcgaagct atctacactt ctggtcagaa tatcctggtc tcagcatcgg
     3901 ctggttctgg aaagaccttt gtcatggtag agcgcattct ggaccaattg gcgcgtggtg
     3961 tcgaaatttc tcaactcttt atctcaacct ttaccgtcaa ggctgcaact gaacttaaag
     4021 aacgtttaga gaaaaaaatc agcaagaaaa tccaagaaac agatgatgtc gatctcaaac
     4081 aacacttggg tcgccagttg gcagacctac ccaacgctgc catcggaacc atggactctt
     4141 tcacacaaaa attccttggc aaacatggtt atctgcttga tattgcacct aatttccgta
     4201 ttttacaaaa ccaaagcgag caacttcttc tcgaaaacga agtctttcat gaggtctttg
     4261 aagcgcatta ccaaggtaaa cagaaagaga gctttagtca tttgctgaaa aactttgctg
     4321 ggcgtggcaa ggacgaacgg ggtctgcggc agcaagtcta taaaatctat gacttcctcc
     4381 aatccaccag taatcctcaa aagtggctga gtgaatcttt cctcaaaggc tttgagaaag
     4441 ctgattttac cagtgaaaaa gaaaaactga ctgagcaaat caaacaagcc ctctgggatt
     4501 tggaaagctt tttccgttac catctggata acgatgccaa ggagtttgca aaggctgcct
     4561 atttagaaaa tgttcagtta attctggatg aaattggctc cctaaatcag gagttcgata
     4621 gtcaggctta tcaggcagta cttgcgcgtg ttgtcgccat ctctaaggag aaaaacggtc
     4681 gagctctgac taatgccagc cgtaaggctg atttgaagcc cctggctgat gcctacaacg
     4741 aagagagaaa gacccagttt actaaactag gacaattatc agaccagata gcgattctcg
     4801 actatcaaga acgttatcat ggagacactt ggaaactagc taaaaccttc caatctttca
     4861 tgagcgattt tgtagaggct tatcgtcaga gaaaacgaca ggaaaatgcc tttgaattcg
     4921 ctgatatcag ccattacacc attgagattt tagaaaattt cccacaagtt cgtgagtctt
     4981 atcaggagcg cttccatgaa gtcatggtcg atgagtatca ggatatcaac catattcaag
     5041 aacggatgct ggaattgttg tctaatggcc acaatcgctt tatggtggga gatatcaagc
     5101 aatccatcta tcgtttccgt caggcagacc cgcagatttt caatgagaaa ttccaacgct
     5161 atgcgcaaaa tcctcaagaa ggcaagctca ttctcctcaa ggaaaatttc cgtagtagtt
     5221 cagaagtgct gtcagcaacc aatgatgtct ttgaacgtct catggaccaa gaggtcggcg
     5281 aaatcaacta tgataacatg caccagcttg tttttgccaa taccaaactg actcccaatc
     5341 cagacaacaa ggccgaattt ctcctctacg acaaggacga tacaggtgag gaagaagaga
     5401 gtcaaacaga aacgaaacta acaggcgaaa tgcgcttagt tatcaaggag atccttaaac
     5461 ttcatcagga aaaaggtgtt gcctttaagg aaattgccct tctgacctct agtcgtaatc
     5521 gtaatgacca aattctactt gccctgtctg agtacgggat tcctgtcaaa actgacggag
     5581 agcaaaacaa ttatctccaa tccctagaag tgcaagtcat gctagacacc cttcgtgtca
     5641 ttcacaatcc cctgcaagac tacgccttgg ttgcccttat gaagtctcca atgtttggtt
     5701 ttgatgagga tgagctagca cgtttgtccc ttcagaaagc agaggataaa gcccacgaaa
     5761 atctctatga gaaactagtc aatgcacaaa aaaaggcaag tagtcaaaaa ggcttgattc
     5821 acacagctct agctgaaaaa ctaaagcaat tcatggatat cctagcttct tggcgcttgt
     5881 atgccaaacc ccactctctc tatgacttga tttggaagat ttacaacgac cgtttttatt
     5941 atgactatgt tggggctttg ccgaatggtc ctgctaggca ggccaatctc tatgccctag
     6001 cactgcgtgc tgatcaattt gaaaagagca atttcaaagg tttgtcgcgt tttattcgta
     6061 tgattgacca agtcttagaa gcccagcacg atttggcaag cgtggccgtc gcaccgccaa
     6121 aagatgcaat agagctcatg accatccaca agagtaaagg gctggagttt ccttacgtct
     6181 ttatcctcaa tatggatcaa gatttcaaca aacaagactc tatgtcagaa gtcattctca
     6241 gtcgtcagaa tggtcttggt gtcaaatata ttgccaagat ggagacaggg gcagtagaag
     6301 accactatcc taaaaccatc aaactctcca ttcctagtct gacctatagg cagaacgaag
     6361 aggaattaca gctagcaagc tattctgagc agatgcgttt gctgtatgtt gctatgacgc
     6421 gggctgagaa aaagctctat cttgtcggca agggttctcg tgaaaagctg gaatccaagg
     6481 aatacccagc agccaaaaat gggaaactaa atagcaatac tagactgcaa gcacggaatt
     6541 tccaagattg gctttgggct atcagtaaag tgtttactaa ggacaagctc aactttagtt
     6601 atcgttttat tggcgaagat cagttgacca gagaagctat cggagagttg gaaaccaaga
     6661 gtcctctcca agatagctcc caagcagaca atcgtcagtc agataccatc aaagaagctc
     6721 tggaaatgct gaaggaggtg gaagtttata atactcttca ccgcgcagct attgaacttc
     6781 ctagtgttca aaccccaagt caaatcaaga aattctacga accagttatg gatatggaag
     6841 gtgtcgagat tgctggtcaa ggtcagtcag taggcaagaa aatcagcttc gatttgccag
     6901 atttttcaac caaagaaaag gtaactggag ctgagattgg tagtgctact cacgaactca
     6961 tgcagagaat tgacctcagc cagcaactaa cccttgctag cctaacagaa acactcaaac
     7021 aagttcaaac tagccaagct gtcagagaca agatcaatct tgataaaatt cttgctttct
     7081 ttgacacagt actcggtcag gaaattcttg ctaataccga ccatctttat cgcgagcaac
     7141 ctttctccat gctcaaacga gaccaaaaga gtcaggaaga ctttgttgtc cgtggtatcc
     7201 ttgatggcta tctgctttac gaaaacaaaa ttgttctgtt cgactacaag acagaccgct
     7261 atgatgaacc aagtcaactc gtagaccgct atcgtggtca gttagctcta tacgaagagg
     7321 ctttatcacg agcctattcg attgaaaata ttgaaaaata cttgatttta ctcggtaaag
     7381 acgaggttca agttgtaaaa gtataactta tactctttga aaatctcttc aaaccacgtc
     7441 agcttcgcct tgctgtatat atggttactg acttcgtcag ttctatccac aacctcaaaa
     7501 cagtgttttg agctgacttc gtcagttcta tctacaacct caaaacagtg ttttgagctg
     7561 attgcggcta gctttctagt ttgctctttg atttttattg agtattagaa aggagacctc
     7621 atgacacttc cagttagaaa atccctgcac gatgcagttt tacaggcttc gaaagcggat
     7681 acttgggaac aagctaccaa ggaatggaat gaagtttcct ttatttttaa cggtatcggc
     7741 cgtagcaatt gtgtctgtgg aaatgccatc aaatacgctt atgaactctt taacggtgtc
     7801 acaggccaac gcctctttcc aattggtagc gactgtgttc gtcattttca tcgattgtcc
     7861 cttgaccagc aattggaaga ggaagaaaaa ctgctcagaa aggttgaaaa tctaaccaga
     7921 aaagctcaga aaaaggaaaa aatcaaggtc aataaaagta actttgacga gcgacttcta
     7981 aaatggttct gggaaaaagg tgttttcaaa cccaatcgtg gcaatcaatt tgcgcctgag
     8041 agagactacc agcttttcct agaagtcttt cagggaggaa gttggaccaa ggcggaacca
     8101 aagaaaaagg ctcggatgga agaagtcctt gaaaagtgta tcaaaccctt tttacttggg
     8161 aaatcggatg accaactcta ccttgtcaag ctaggcaagg agaaaattga ctacgaacaa
     8221 gaactccgta tccaggcaga gaaagaacgc aagaagaggg ataaaatcgc caagcaatat
     8281 gcagacaatc tggttctagc catgggaccc gcagagcgtg cttatcaaga ttactttggc
     8341 tttacagaga ccctgactca agaagaacgc aagtgggaaa aaattctttt tggtaaaaat
     8401 agagcagaac gagctatcaa ggccaaacaa taccaaaaag agctggaaaa agaccaacga
     8461 attgccagtc aggatccaat tgaaagaaaa cagaagcaga cttggatcct caattcctat
     8521 tttcgtgagc ttcctgaaga aaaagctcga ttttctagac tcttattaga atatcgaaaa
     8581 agtggtgaag tacccttttc aactgaatac ctgtcagatc atctcatcga ctttttctat
     8641 aaaatgaaag cctttgaatt tgaaatcgca ccagaacaag tccgagattt tctaaaaaaa
     8701 agcctgcagg aagaccatcg ctcatcagca caaggaagct ggatagaagg aattctcctc
     8761 aactgcctca aaccattttt agaacgatta gtgatatgag taacacctac cgtgtaaatt
     8821 tcatggtagg tgttattgta cccccaaata atataaaaca aagtatgaaa gcgcatctca
     8881 attttaagac ttttttgtaa attaagagta taattaaggt atactg
//
LOCUS       DAVHEW010000086         8910 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVHEW010000086 DAVHEW010000000
VERSION     DAVHEW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.086"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            139..3240
                     /locus_tag="VVL53_001729"
     CDS             139..3240
                     /locus_tag="VVL53_001729"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4183868.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNVFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3322..4329
                     /gene="pfkA"
                     /locus_tag="VVL53_001730"
     CDS             3322..4329
                     /gene="pfkA"
                     /locus_tag="VVL53_001730"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU4183869.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4388..5893
                     /gene="pyk"
                     /locus_tag="VVL53_001731"
     CDS             4388..5893
                     /gene="pyk"
                     /locus_tag="VVL53_001731"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU4183870.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6164..6457)
                     /locus_tag="VVL53_001732"
                     /pseudo
     CDS             complement(6164..6457)
                     /locus_tag="VVL53_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(6624..6849)
                     /locus_tag="VVL53_001733"
                     /pseudo
     CDS             complement(6624..6849)
                     /locus_tag="VVL53_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7697..8569
                     /locus_tag="VVL53_001734"
     CDS             7697..8569
                     /locus_tag="VVL53_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU4183871.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8715..8910)
                     /locus_tag="VVL53_001735"
                     /pseudo
     CDS             complement(8715..8910)
                     /locus_tag="VVL53_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         2727 a         1553 c         1917 g         2713 t
ORIGIN      
        1 agtataaata taaaaatgat gacaaaaata caaatgaata gaaaataaat tagtaagctg
       61 atgaaatttt tctcaagaga agccatttat aggtgaaaat ggtataatat agtgagaagg
      121 atagaggaga agtgtaaatt gatcgcacaa ctagatacaa aaacagtcta tagttttatg
      181 gaaagcgtca tttcgatcga aaagtatgtg agagcagcta aagaatacgg ctacactcat
      241 ttggctatga tggatattga caatctttat ggcgctttcg actttctaga gattacaaaa
      301 aaatacggca ttcatccttt gctagggctt gaaatgacag tgtttgtaga tgatcaggga
      361 gtgaatttgc gctttttagc tctatctagt gtgggctatc agcagttgat gaagctttcg
      421 acagccaaga tgcaggggga gaaaacttgg tcagtcctgt cccagtacct ggaggatatc
      481 gcggtcattg tgccttattt tgatagagtt gagtcgttag aactaggctg tgattactat
      541 ataggggttt atccagaaac actagcaagc gaatttcatc atcctatctt acctctttat
      601 cgggtcaacg tttttgaaag cagggataga gaagttcttc aagttttaac agcgattaaa
      661 gaaaatctac cgctcagaga agttcccttg cgttcgagac aagatgtctt tatatcagca
      721 agttctttag agaaactatt ccaagagcgt tttccgcaag ctttggacaa tttagaaaag
      781 cttatttcag gcatttctta cgacttggat actagtctga aactgcctcg ttttaatcca
      841 gctagaccag cagtagagga gttgagagag cgtgctgaac tggggcttgt tcagaagggg
      901 ttgactagta aagaatatca agatagacta gaccaagaat tgtctgttat tcatgatatg
      961 ggctttgatg attatttctt ggttgtttgg gatttgttgc gttttggaca atcgaatggc
     1021 tattatatgg gaatgggaag gggttctgca gtaggcagtt tggtttctta tgccttagac
     1081 atcacgggga ttgacccagt agagaaaaat ctgatttttg aacgctttct taatcgtgaa
     1141 cgctatacca tgcctgatat tgatattgat atcccagata tttatcgtcc agattttatc
     1201 agatatgttg gtaataaata tggtagtaaa catgcggcac aaatcgttac tttttcaacc
     1261 tttggagcca agcaagctct tcgagatgtc ttgaaacgct ttggtgtgcc agagtatgaa
     1321 ttatctgcaa ttactaagaa aatcagtttt cgtgacaatc ttaagtcggc ctatgaggga
     1381 aatctccagt ttcgtcagca aatcaatagt aagttagaat accaaaaagc ttttgagatt
     1441 gcttgcaaga tagagggcta tccaaggcaa acctctgtcc atgcggctgg tgttgtaatt
     1501 agtgaccaag atttaaccaa ctacattcct ctaaagtatg gtgatgaaat tccactgact
     1561 cagtatgatg ctcatggagt tgaggctagc ggacttttga agatggactt tctgggacta
     1621 cgaaatttga cctttgtcca gaagatgcaa gagttgcttg ctgaaacaga aggtattcat
     1681 ctgaaaattg aagaaatcga tttagaagac aaagaaacgt tagctttatt tgcctctggt
     1741 aatacaaaag gtatctttca atttgagcaa ccaggtgcca ttcgtctgct taagcgtgtg
     1801 caaccagtct gttttgaaga tgtcgtcgcg actacttctc taaatcgacc gggtgctagt
     1861 gactatatca ataattttgt ggcaagaaag catgggcagg aagaagtgac tgttctggat
     1921 ccagtactgg aggatatttt ggctccaacc tacggcataa tgctctatca ggagcaggtt
     1981 atgcaggttg cccagcgact tgccggattt agtcttggga aagccgatat tttgcgtcgg
     2041 gctatgggga aaaaggatgc ctctgccatg catgagatga gggcttcctt tattcaaggt
     2101 tcattagaag ctggtcatac tgtggaaaaa gcagagcagg tctttgatgt tatggagaag
     2161 tttgcaggtt atggttttaa caggtcacac gcctatgcct actcagcctt ggccttccag
     2221 ttggcttatt tcaaaacgca ttatccagcc attttttatc aggtcatgtt aaattcttcc
     2281 aacagtgatt acttaataga tgcacttgaa gcaggttttg aagtagcctc tctatccatc
     2341 aacaccattc cctatcacga taaaattgcc aacaaggcca tctatctagg tttgaaatcc
     2401 attaaaggag tcagtaatga tttagctctc tggattattg aaaatagacc ttattctaac
     2461 attgaagatt ttatagctaa attacctgag aattatctga aacttcctct gctagaacct
     2521 ttggtaaaag ttggtctttt cgattcattt gaaaaaaatc gtcaaaaagt atttaataac
     2581 ttagctaatc tatttgaatt tgtgaaagag ttgggaagtt tgtttggaga tgctatttat
     2641 agttggcagg aatcggaaga ttggacggaa caagaaaaat tttatatgga acaagagctt
     2701 ttagggatag gtgtcagcaa acatccacta caagctattg caagtaaggc tatttacccg
     2761 attaccccaa tcggaaattt gtcagaaaat agctatgcta ttatcttggt tgaagttcag
     2821 aaaataaaag tgattcgtac caaaaagggt gaaaatatgg ccttcttaca ggcagatgat
     2881 agtaagaaaa aattggatgt cactctcttt tcagacttat atcgtcaggt tggacaggaa
     2941 ataaaagagg gagccttcta ctatgtaaaa ggaaaaatac aatcacgtga tggccgtctg
     3001 caaatgattg cacaagaaat aagagaagca gttgctgaac gcttttggat acaggtgaaa
     3061 aatcatgaat cggatcaaga aatttcacgc attttagaac aatttaaagg cccaatccca
     3121 gtcatcatcc ggtatgaaga ggaacagaaa accatcgttt ctccccatca ttttgtagct
     3181 aaatccaatg aattagagga gaaattgaat gaaatcgtta tgaaaacgat ttatcgctaa
     3241 aaatacggaa aatagaagaa ttttcaacgt aaatgtggta taatcagtaa gaatgttaaa
     3301 agaaaaagga gcataaccaa tatgaaacgt attgctgttt tgactagtgg tggagacgcc
     3361 cctggtatga acgctgccat ccgtgcagtt gttcgtcaag caatttcaga aggaatggaa
     3421 gtttttggta tctatgacgg atatgctggt atggttgccg gtgaaattca tcccctagat
     3481 gcagcttcag taggggacat catttctcgt ggtggtactt tccttcactc agctcgttac
     3541 ccagagttcg ctcaacttga agggcaactt aaagggattg agcaattgaa aaaacacgga
     3601 attgaaggtg tagttgttat cggtggtgac ggatcttacc acggcgctat gcgtttgact
     3661 gaacatggct tcccagctat tggtcttcca ggtacaatcg ataacgatat cgttggtact
     3721 gactttacaa tcggttttga cacagcggtt actactgcca tggacgctat cgataagatt
     3781 cgtgatacat catcaagtca ccgtcgtact tttgtaatcg aagttatggg acgtaacgct
     3841 ggtgatatcg ctctttgggc tggtattgca actggtgctg atgaaatcat catccctgaa
     3901 gcaggcttca agatggaaga tatcgtagca agcatcaaag ctggttatga atgtggtaaa
     3961 aaacacaata ttatcgtctt agctgaaggt gtgatgtcag cggctgaatt tggtcaaaaa
     4021 cttaaagaag ctggagatac aagcgacctt cgtgtaacag aacttggaca tattcaacgt
     4081 ggtggttctc caactgcgcg tgaccgtgtt ttggcgtcac gtatgggtgc acatgctgtt
     4141 aaacttctta aagaaggtat cggtggtgtt gcggttggta ttcgtaacga aaaaatggtt
     4201 gaaaatccaa ttcttggtac tgcagaagaa ggggcattgt ttagccttac tgcagaaggt
     4261 aagattgtgg ttaacaaccc acacaaagcc gatattgagc tatctagctt gaataagagc
     4321 ttgtcataat ttacaattta gttaataaga ccaaaaaggt catatatata aaggagtcac
     4381 aaaaatcatg aacaaacgtg taaaaatcgt tgcaactttg ggtcctgcgg tagaaatccg
     4441 tggtggtaaa aaattcggtg aggacggata ctggggtgaa aaacttgatg ttgaagcttc
     4501 agctaaaaac attgctaaat tgattgaagc tggtgctaac acattccgat tcaacttctc
     4561 acacggcgac caccaagaac aaggtgagcg tatggcaact gttaaacttg cggaaaaaat
     4621 tgcaggtaaa aaagttggtt tccttcttga tacaaaagga cctgaaatcc gtacagaatt
     4681 gttcgaaggt gaagctaaag aatattcata caaaactggt gaaaaaattc gtgttgcaac
     4741 taaacaagga atcaaatcaa ctcgtgaagt gattgcgttg aacgttgctg gtgctcttga
     4801 tatctatgat gatgttgaag ttggtcgtca agttttggtt gacgatggta aacttggtct
     4861 tcgtgtggtt gctaaagatg atgcaactcg tgaatttgaa gttgaagttg aaaacgatgg
     4921 tatcatcgct aaacaaaaag gtgtgaacat ccctaacact aaaattcctt tcccagctct
     4981 tgctgaacgc gataacgacg atatccgttt cggtcttgaa caaggtatca acttcatcgc
     5041 aatttcattc gtacgtactg caaaagatgt gaacgaagtt cgtgcaatct gtgaagaaac
     5101 tggaaacgga catgttcaat tgttcgctaa aatcgaaaac caacaaggta tcgataactt
     5161 agatgaaatc atcgaagcag ctgatggtat tatgattgct cgtggtgata tgggtatcga
     5221 agtaccgttc gaaatggttc cagtttatca aaaaatgatt atcaagaaag tcaatgctgc
     5281 aggtaaagtt gttatcactg caacaaacat gcttgaaaca atgactgaaa aaccacgtgc
     5341 aactcgttca gaagtatcag atgtattcaa cgctgttatc gacggaactg acgctacaat
     5401 gttgtcaggc gagtctgcaa acggtaaata cccactcgag tcagtaacta caatggctac
     5461 aatcgacaag aacgctcaag ctcttcttaa tgaatacgga cgtcttgatt cagattcatt
     5521 tgagcgtaac tctaagacag aagtaatggc ttctgctgtt aaagatgcta ctagctcaat
     5581 ggatatcaaa ttggttgtaa ctcttactaa gacaggtcat actgcacgtt tgatttctaa
     5641 ataccgtcca aatgctgaca tcttagcatt gacatttgac gaattgacag aacgtggctt
     5701 gatgttgaac tggggtgtta tcccaatgtt gacagatgct ccatcttcaa ctgacgatat
     5761 gttcgaaatc gctgaacgta aagcggtaga agcaggtctc gttgagtcag gcgatgatat
     5821 cgttatcgtt gctggtgtgc cagtaggaga agctgttcgc acaaacacaa tgcgtatccg
     5881 cacagtacgt taagaaaaat ataaaaacct atcatatcca gctttagagc ttgtgtgata
     5941 ggctttttgt atagagggta agaaataggc aaaactttca taatggattg atactcttcg
     6001 aaaatctctt caaaccacgt cagcgtcgcc ttaccgtata tatgttactg acttcgtcag
     6061 ttctatctac aacctcaaag cagtgctttg agcaacctgc ggctagcttc ctagtttgct
     6121 ctttgatttt cattgagtat gaaataagat atgcacaaat tgattagaaa gtcaaatgaa
     6181 tttctacaaa tgttttagca atcgtaatgt acttgtctag attcgatctg atatattttc
     6241 gatttaatga tatggtattt aaaacctcca aagtagctta ctccattctt ttacttacgt
     6301 gagtgtagat gttatttact gttttagcgt ttttgtgttc cactctaacc attatagcat
     6361 tcttctcagc tagtgtacta aggagtgtgt gcctgaaaat atgggaacta aggggctggt
     6421 ttatcggttt ctctagttta gtatttgcct tttgcaaagt gatcttaaat gcctttctct
     6481 aaatttacat atcactattg tttaacaaaa tctaatctat tttaggtcac ttattctttt
     6541 tttgaaatgt agaatgaact ttttcaaagt ttttcgaatc ttttaaaatc tgtttgcttt
     6601 atatcgccat tctcccccct tttttaattc tccctatata gcctgacagc tttcccgatg
     6661 gtacgaatat ggttgctttc gtctaggtgg atgtcggggt attcgggatt gagttttttt
     6721 gaggcagcct tggcggagtt tcttgacata gttagtgccg tctacttgga agatgccgat
     6781 ggtattatag tcaatctgtg gggtattctt gataaatagg tagtcgctgt ttcttatctt
     6841 tggctccatg gacttgctga cgacataagc gattgggtcg tagtcgtctg ggataatgga
     6901 aactccatat ctaaatcgtt gtcctgcatc gagcggctac ctgcagagat aaactaccta
     6961 acacgagagt aagtagtctg tctgtagtcg tccagtctga tgatttttac gatacttcgt
     7021 ttttctgatc atacagttgc ctctcggcat aggtcagaac tttaccttgt ctgggtggtt
     7081 cccgttggtc gtagatagat tggatatcgc taggagaatc cttttgaact ggaggaaaga
     7141 gggcatcgat caagctactg aatactttaa ctaagtcaaa tatagtattt ttcttagtag
     7201 acctaaccct tttttcataa tttctaatgg tgtttttact tatacctatc ttagtaccca
     7261 attcttattg agtccaacca ttactagtct atattgtttt atagttgatt gagtttggaa
     7321 tagtacgctg tagctgctaa aacatttcta gaaattaatt tgactttcct aatagagttg
     7381 ttcatatctt atttcaatct attatgtttt tcacctctaa caatcgcaat ctcttcttta
     7441 tacatgaatg aaatcgcttt ctatttttgt aagtaaagca taacacgaaa tccacgaaaa
     7501 tgaaaacctt tgttgtgttt tcgtaaaaaa tttgttgaca gagcacgaaa cgcctactat
     7561 aattaaatca agcttaagga aataacaaaa aaacaaatcg gagggaaaga cgatgaacac
     7621 attaaatgag aaagtaatca atatctgtaa agcaatagtt aaagaaactt taatccaaga
     7681 catttaggag aaagaaatga agaaatcaag aaaattagcc acattaggaa tctgttcagc
     7741 cttgttttta ggcttggctg cttgtcaaca acaacatgct acttctgagg ggacgaatca
     7801 aaggcaaagc agttcagcga aagttccatg gaaagcttca tacaccaacc taaacaacca
     7861 ggtaagtaca gaagaggtca aatctctctt atcagctcac ttggatccaa atagtgttga
     7921 tgcatttttt aatctcgtta atgactataa taccattgtc ggctcaactg gcttatcagg
     7981 agatttcact tcctttactc acaccgaata cgatgttgag aaaatcagtc atctctggaa
     8041 tcaaaagaag ggcgatttcg ttgggaccaa ctgccgtatc aatagttatt gtcttttgaa
     8101 aaattcagtc accattccaa agcttgaaaa gaatgaccaa ttgcttttcc tagataatga
     8161 tgcgattgat aaaggaaagg tctttgattc acaagataag gaagagtttg atattctatt
     8221 ttcgagagtt ccaactgagg caactacaga tgtcaaggtt cacgctgaaa agatggaaac
     8281 attcttctca caatttcaat tcaatgaaaa agctcgaatg ctgtctgtag tcttgcacga
     8341 caatttggat ggcgagtatc tgtttgtagg ccacgttggg gtcttagtac ctgctgatga
     8401 cggtttctta tttgtagaga aattgacttt cgaagagccc taccaagcga ttaaatttgc
     8461 tagtaaggaa gattgctaca agtatttggg caccaagtat gcggattata caggcgaggg
     8521 actggctaag ccttttatca tggataatga taagtgggtt aaactttaat agtgttatga
     8581 tatgacagta atcttaggat ttttatttct atttttgatg agttggtggg ttatttcaat
     8641 cgttaatagc cagaaaccac gcaaaaatga aacatttatt ggctatatcc aaagatacga
     8701 tattgatggg aatggctatc aatcattacc gtgtcctcag aactgagagg agttcttgaa
     8761 atcgtaaaac cactttgaac aagagttact tcaacccatt ggctctgatg gattaagttg
     8821 ttttcgtgaa taccaaaatc agccgcaatt tcttcataag tgcggtattc tcgcacatat
     8881 tgaagagtgg gcataagaag gtcttctagg
//
LOCUS       DAVHEW010000087         8830 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVHEW010000087 DAVHEW010000000
VERSION     DAVHEW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.087"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..760
                     /locus_tag="VVL53_001736"
     CDS             <1..760
                     /locus_tag="VVL53_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEU4183872.1"
                     /translation="TLYFYLSPISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKK
                     ETNNIVPGVAIATALMPPLCTVGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRL
                     MMVKKHYFKDNEEDSKMRRILLLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQ
                     GYNILKKTYSKKTHTLKLTISGNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQL
                     SKEELVEHFFQYIKDKEAKEKEKANKFDTESEEQ"
     gene            927..3014
                     /gene="topA"
                     /locus_tag="VVL53_001737"
     CDS             927..3014
                     /gene="topA"
                     /locus_tag="VVL53_001737"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU4183873.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3235..3594
                     /locus_tag="VVL53_001738"
     CDS             3235..3594
                     /locus_tag="VVL53_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU4183874.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            3687..4319
                     /locus_tag="VVL53_001739"
     CDS             3687..4319
                     /locus_tag="VVL53_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU4183875.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4363..5019
                     /locus_tag="VVL53_001740"
     CDS             4363..5019
                     /locus_tag="VVL53_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU4183876.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESIRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5038..5670
                     /locus_tag="VVL53_001741"
     CDS             5038..5670
                     /locus_tag="VVL53_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU4183877.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            5698..6132
                     /locus_tag="VVL53_001742"
     CDS             5698..6132
                     /locus_tag="VVL53_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU4183878.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQIHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINVVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6144..7181
                     /gene="leuB"
                     /locus_tag="VVL53_001743"
     CDS             6144..7181
                     /gene="leuB"
                     /locus_tag="VVL53_001743"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU4183879.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVVMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7178..7447
                     /locus_tag="VVL53_001744"
     CDS             7178..7447
                     /locus_tag="VVL53_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU4183880.1"
                     /translation="MKLDEKIALVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            7451..7810
                     /locus_tag="VVL53_001745"
     CDS             7451..7810
                     /locus_tag="VVL53_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU4183881.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPIDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNTLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            7972..8542
                     /locus_tag="VVL53_001746"
                     /pseudo
     CDS             7972..8542
                     /locus_tag="VVL53_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            8636..8701
                     /locus_tag="VVL53_001747"
                     /pseudo
     CDS             8636..8701
                     /locus_tag="VVL53_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2725 a         1583 c         1981 g         2541 t
ORIGIN      
        1 aacactttat ttttatcttt ctcccatttc gtatgctagt tcggaaattg ttgctagaac
       61 ctctccgact atttgggatg ttctcattgc ttttgtagga gggatagcag gtattattgg
      121 tgctaggaaa aaagagacca ataatattgt tcctggtgtt gctattgcaa ccgccttgat
      181 gcctcctctt tgtacagtag gttatgctat tgcttctgct aatctaaaat ttatcatagg
      241 ctcctcttac ctattcctca tcaattgtag ctttattgtc attgcgactt atataggtgt
      301 taggttgatg atggttaaga aacattattt taaagataat gaagaagact ctaaaatgcg
      361 taggattttg cttctagttg ctgttttgct gatgattccg agtttcatct ctgcaacgac
      421 tttagtgaga gaaacgttga aaaaagagtc ccttaagaaa tttatatcag agcagtttca
      481 ggggtataat attttgaaaa aaacctattc taaaaagact cataccctaa agctaaccat
      541 ttcaggaaat tatttgacag aagaagaact cgatatgatt tcaagtaaga gaggtgacta
      601 tggtttaagt gatgtttctg ttcaagtttc acaattgtct gattcagaac aacttagtaa
      661 ggaagagctg gtggagcatt tcttccagta tatcaaggat aaggaagcaa aagaaaagga
      721 gaaagccaat aaatttgata cagagtctga ggagcaataa tttcttgaga atagctggtt
      781 tctctcgtga gtcttctatg tatatcaaag gaagactgag gttttaagta taaaactttt
      841 cttctattat agtagctaag tcttgacagg gttcaaaaaa tggtttacac tttataaagt
      901 ttattacttt gaaaaggtgt gatactgtgg ctacggcaac aaaaaagaaa aaatcaacag
      961 ttaaaaaaaa tctagtcatc gtggagtcgc ctgctaaggc caagacgatt gaaaaatatc
     1021 taggcagaaa ctacaaggtt ttagccagtg tcgggcatat ccgtgatttg aagaaatcca
     1081 gtatgtccgt cgatattgaa aataattatg aaccgcaata tattaatatc cgaggaaaag
     1141 gccctcttat caatgacttg aaaaaagaag ctaaaaaagc taataaagtt tttctcgcga
     1201 gtgacccgga ccgtgaagga gaagcgattt cttggcattt ggcccatatt ctcaacttgg
     1261 atgaaaatga tgccaaccgt gtggtcttca atgaaatcac caaggatgca gtcaaaaatg
     1321 cttttaaaga acctcgtaag atcgatatgg acttggtcga tgcccaacaa gctcgtcgga
     1381 tcttggatcg cttggtaggg tattcgattt cgcctatttt gtggaagaag gtcaagaagg
     1441 gcttgtcagc aggtcgcgtt cagtccattg cccttaaact catcattgac cgtgaaaatg
     1501 aaatcaatgc cttccagcca gaagaatact ggacagttga tgctgtcttt aaaaagggaa
     1561 ccaaacaatt tcatgcttcc ttctatggag tagatggtaa aaagatgaaa ctgaccagca
     1621 atgacgaagt caaggaagtc ttgtctcgtc tgactagtaa agacttttca gtagatcagg
     1681 tggataagaa agagcgcaag cgcaatgctc ctttacccta taccacttca tctatgcaga
     1741 tggatgctgc caataaaatc aatttccgta ctcgaaaaac catgatggtt gcccaacagc
     1801 tctatgaagg aattaatatc ggttctggtg ttcaaggttt gattacctat atgcgtaccg
     1861 attcgactcg tatcagtcct gtagcgcaaa atgaggcggc aagcttcatt acggatcgtt
     1921 ttggtagtaa gtattctaag cacggtagca aggtcaaaaa cgcatcaggt gctcaggatg
     1981 cccatgaggc tattcgtccg tcaagtgtct ttaatacacc agaaagcatc gctaagtatc
     2041 tggacaagga tcagctcaag ctatataccc ttatctggaa tcgttttgtg gctagccaga
     2101 tgacagcggc cgtttttgat accatggctg ttaaattgtc tcaaaaaggg gttcaatttg
     2161 ctgccaatgg tagtcaggtt aagtttgatg gttatcttgc catttataat gattctgaca
     2221 agaataagat gttaccggac atggttgttg gagatgtggt caaacaggtc aatagcaaac
     2281 cagagcaaca tttcacccaa ccgcctgccc gttattctga agcaacactg attaaaacct
     2341 tagaggaaaa tggggttgga cgtccatcaa cctacgcgcc aaccattgaa accattcaga
     2401 aacgttatta tgttcgcctg gcagccaaac gttttgaacc gacagagttg ggagaaattg
     2461 tcaataagct catcgttgaa tatttcccag atatcgtaaa cgtgaccttc acagctgaaa
     2521 tggaaggtaa actggatgat gtcgaagttg gaaaagagca gtggcgacgg gtcattgatg
     2581 ccttttacaa accattctct aaagaagttg ccaaggctga agaagaaatg gaaaaaatcc
     2641 agattaagga tgaaccagct ggatttgact gtgaagtgtg tggcagtcca atggtcatta
     2701 aacttggtcg ttttggtaaa ttctacgctt gtagcaattt cccagattgc cgtcataccc
     2761 aagcaatcgt gaaagagatt ggtgttgagt gtccaagctg tcatcaggga caaattattg
     2821 agcgaaaaac caagcgtaat cgcctattct atggttgcaa tcgctatcca gaatgtgaat
     2881 ttacctcttg ggacaagcct gttggtcgtg actgtccaaa atgtggcaac ttcctcatgg
     2941 agaaaaaagt ccgtggtggt ggcaagcagg ttgtttgtag caaaggcgac tacgaggaag
     3001 aaaagattaa ataagaggag gggcccgaaa gtaaatttca ggctcctttt ggttagaact
     3061 tgacaaaata catcatttta tgcgaaacta gaagaagatt attttatact caatgagaat
     3121 caaagagcaa attagaaagc tagtcgcagg ttgctcaaag cactactttg aggttgaaga
     3181 tggaagctga cctggtttga agagattttc gaagagtatt aactaggaga agttatgcgt
     3241 cttatctatc taattattgg ttttttatcg ctggccttgg ctattgttgg ggttgtttta
     3301 cccttgttgc cgacaacgcc tttccttttg ttgtctattg cttgtttctc cagaagttcc
     3361 aagcgtttcg aagactggct ttatcatacc aagctctatc aaacatatgt agctgatttt
     3421 cgcgagacta agtctattac gcgtgaacga aagaaaaaaa tcatcgtctc tatctacgtc
     3481 ttgatgggaa tttctattta ttttgcacct cttttaccag tcaaaatcgg tctgggtgct
     3541 ttgaccatct ttatcactta ttatctcttc aaggtcattc cagacaaaga atagttaaaa
     3601 cagtagttat ttgccttgat aaaattgaaa gcatattcat aacaatatga tataataaat
     3661 ttgaagtaat attcaaggag aatcaaatga tttacgaatt ttgtgctgaa aatgtgactt
     3721 tacttgaaaa agcgatgcag gctggagctc gtcggattga actctgtgat aatctagcag
     3781 ttggtgggac aacacccagc tatggagtga ctaaggcagc ggttgaactg gcagctaact
     3841 acgatacaac catcatgacc atgattcggc cacgtggtgg tgactttgtc tataatgacc
     3901 tagaaattgc tatcatgcta gaagacattt gtttgactgc tcaggctgga agtcaagggg
     3961 ttgtatttgg agctttaact gctgataaaa agttggataa gcctaatctg gaaaagttaa
     4021 ttgctgcatc aaaaggaatg gaaattgtct ttcacatggc ctttgatgaa ctaagtgatg
     4081 aagatcaacc ggaagctatt gactggctca gtcaagccgg tgtcactcgt atcctaactc
     4141 gtgctggtgt gtctggcgac tccttagaaa aacgttttgt tcactatcac agaattttgg
     4201 agtacgctaa aggtaaaatt gaaattctac caggtggggg gattgacctt gaaaaccgtc
     4261 aaacctttat cgaccaggtg ggggtaacac aattgcatgg tactaaggtt gttttttaaa
     4321 aaatagaaag gaactgctag ccttgggtag cagttttcac ttatgtttga aatttttaaa
     4381 tcctatcaat ttaatcaaga aaaggctcat gattatggtt ttatagaaaa tagcgaagtc
     4441 tggacatata gttgccagat tttgcaaggt gactttgtca tgactgtgtc catcactgct
     4501 gataatgtga actttcaagt ctttgaccaa gagactggtg acctctatcc tcacgttcat
     4561 atggaaagca taaggggaag ttttgtcgga aatgtccgtg aggcttgtct ggagattctt
     4621 taccagattc ggaaggcttg ttttgatgtg caagatttta tctgtcatca gactaagcgt
     4681 atcatgactc aagttcagga aaagtatgga aaccagttgg agtatctgtg ggaaaaatcg
     4741 cctgatacag ctgtattgcg ccatgaaggc aatcaaaagt ggtatgccgt cttgatgaaa
     4801 atctcttgga ataagctgga aaagggcaga gaaggacaag tggaagcagt caacctcaag
     4861 catgaccaag tagctaattt gctttcacaa aaggggattt atccagcctt ccatatgagc
     4921 aagcgctact ggattagtgt gtcccttgat gatactttat cagatgaaga agtactggaa
     4981 ttgatagaaa aaagttggaa cttaacctct aaaaaatgaa atattttaat aattttcatg
     5041 aactttcaat tagctaaata ttctttactg aagaaatttt cagaaaatat aggatttacc
     5101 acaccagagg aatgtggtgc catcttcaaa tacctgattg agaatgtcaa gacggatcgt
     5161 cagatcatct attcgcctca ctgtcatgat gacctcggaa tggcagtggc aaatagcctt
     5221 gctgctgtca agaatggtgc aggacgtgtt gaagggacta tcaatggtat tagggagcga
     5281 gctgaaaatg ctgctttgga agaaattgca gtggctctca atattcgcca agattactac
     5341 caagtagaaa ccagtattgt cctaaatgag accatcaata cgtcagaaat ggtttctcgc
     5401 ttctctggta ttccagttcc taaaaacaaa gccgtcgttg gtggcaatac cttctcccac
     5461 gaatctggta ttcaccaaga tggagtcctt aaaaatcctc tcacttatga gatcatcaca
     5521 cctgaattgg ttggtgttaa gattccgctt ggaaaattat ctggtcgcca tgcttttgtt
     5581 gagaaactga gagaattggc cctagatttt acagaagagg atatcaaacc actctttgct
     5641 aagttcaagg cactggccga taagaagtaa gaaatcacag atgcagatac tcgagctttg
     5701 gtagctggaa ccatggttga aaatccagaa ggcttccact ttgatgattt acaacttcaa
     5761 attcatgcag ataatgacat tgaagcgctc gttagcctag ccaatatgga tggtgagaaa
     5821 gtcgaattta atgcgacagg gcaaggttcc gttgaagcaa tctttaacgc tatcgataag
     5881 ttctttaacc aatctgttcg tttggtgtcc tacactatca atgtggtaac agatggaatc
     5941 gatgcccagg atcgggtttt ggtcactgtt gaaaacagag atacagaaac catctttaat
     6001 gcagcagggc ttgattttga tgtgttgaag gcttctgcta ttgcctatat aaacgctaat
     6061 acctttgttc aaaaagagaa tgcaggtgag atgggacgca gtgtttctta ccacgatatg
     6121 cctagtgtgt aaaggagaag gctatggcaa agaaaatagt agctctagta ggagacggaa
     6181 ttggcccaga aatcatggag gctggtttag aagttctgga ggctctagct gaaaaaacag
     6241 gttttgacta tgagattgac agacgaccgt tcggaggtgc agatattgat gcagcaggac
     6301 ctcccttacc tgatgaaacc cttaaggcaa gtagggaagc agatgctatc ctactagcag
     6361 ctatcggtag tcctcagtat gatggagcag cggttcgccc tgaacaaggc ctgatggctc
     6421 tccgtaagga actcaatctt tacgctaata ttcgtcctgt aaaaatcttt gacagtctca
     6481 agtatttgtc accactcaaa ccggaacgaa tttctggtgt agactttgtc gtggtgcgtg
     6541 aattgacagg cgagatttac tttggagatc atattcttga agagcgcaaa gcgcgtgata
     6601 tcaacgacta tagctatgag gaagtggagc ggattattcg caaagccttt gaaattgcaa
     6661 gaaatcgcag aaaaatcctt actagtatcg ataagcaaaa tgttctagcg acctcaaaac
     6721 tctggcggaa agtagctgag gaagtcgcac aggatttccc agatgtaacc ttggaacacc
     6781 agctggtaga ctcagctgct atgcttatga ttaccaatcc tgctaagttt gatgttattg
     6841 taacggagaa tctttttgga gatattttat ctgatgaatc aagcgtctta tctggtacac
     6901 ttgaggttat gccatcagcc agtcattctg aaaatggacc aagtctctat gaacctattc
     6961 acggttcagc acctgatatt gcaggtcaag gaattgccaa tcctacttcc atgattttat
     7021 cagttgtcat gatgttgaga gatagtttcg gacgttatga ggatgcagag cgtatcaaac
     7081 gtgctgttga gacaagtctg gcggcaggaa ttttaacgag agatatagga ggtcaggctt
     7141 caacaaagga aatgacggaa gctattattg caaggttatg aagttagacg aaaaaattgc
     7201 tctagtcctt ttgatttgga atgtcatcat tttcttgatt tatggcattg acaaattcaa
     7261 ggcaaggaga agagcttggc gcatccaaga gaaaatctta ctcattttag cccttacttg
     7321 tggtggtttt ggggcctggt tagctggaat cacttttcac cacaagactc gaaaatggta
     7381 ctttaaaaca gtttggtttc ttgggatggt gaccacacta gtagccttat attttatttg
     7441 gaggtaatgg atggcagggt cttcgaggga acacgctgct tgggttctag cggactatgg
     7501 ttttaaggtc gtgattgcag gatctttcgg tgacattcat tacaataatg aactcaataa
     7561 tggcctgttg ccaatcgttc agcctagaga ggttagagag aaattagccc agctaaaacc
     7621 aatcgaccag gtaactgtgg acttggaaca acaaaaaatc atctcaccag ttgaagaatt
     7681 caccttcgag atagatagcg agtggaaaca taaactccta aatactttgg atgatatcgg
     7741 tattaccttg cagtatgaag agttgattgc tgcttatgaa aaacaacgac cagcctactg
     7801 gcaggattag aaaaaataga aaaggaaata tagtaaactg aaataagatg taaacaaatg
     7861 aattagagct taacatccat ttccagcaat tttttagaaa ctacagtgga ctattctgga
     7921 ttcaacacat tataaaatta tgacaaaaca cattcacaag aaggctacga cattttaaaa
     7981 ggtgagggcg gatgtatcgt ttgccctact aaagttggtt acattatcat gaccagtgac
     8041 aaggcaggac ttgagcgtaa gttcgcagcc aaagaacgta agcgtaacaa accaggtgtt
     8101 gttctctgcg gtagcatgga tgaacttcgc gctttagcgc aactcaaccc agaaattgaa
     8161 gcattctact aaaaacattg ggatgaagat attcttcttg gttgtatcct tccttggaaa
     8221 ccagaagcct ctgaaaaact caaagcatac ggggatggcc gtgaagaact tatgactgat
     8281 gtacgtggta ctagctgttt tgttatcaag tttggaaaag caggtgaaca attggctgcc
     8341 aagctttggg aagaaggtaa aatggtctac gcctcatctg cttcaatgac aaaacgattg
     8401 aaactcgcta tgagagcaag gtgtaatggt gtctatggtc gataaggacg gcaaactcat
     8461 cccagaacaa ggaggagcac gttcaacttc accagcacca gttgtgatcc gtaaagggct
     8521 tgacattgat atagtaaaat gaagtaagag caggacaaat taatcagaac agtcaaatcg
     8581 atttctaaca atgttttaga agtagaggtg tactattcta gtttcaatcc actataaaat
     8641 catgatgcac ctgtcagata cttttaactc atgggactac cgtcaggttg agtattatta
     8701 ggatagagaa gaagtctagt gttaaggaca ttacagctca taatactagg cttttttata
     8761 gaaattcttt tctttttcta taggatatgg tattctatgt agaaaatata tgttaataag
     8821 taatgccaat
//
LOCUS       DAVHEW010000088         8828 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVHEW010000088 DAVHEW010000000
VERSION     DAVHEW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.088"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            221..2008
                     /locus_tag="VVL53_001748"
     CDS             221..2008
                     /locus_tag="VVL53_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU4183882.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLDKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEERGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2005..2481
                     /locus_tag="VVL53_001749"
     CDS             2005..2481
                     /locus_tag="VVL53_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU4183883.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2509..3414
                     /locus_tag="VVL53_001750"
     CDS             2509..3414
                     /locus_tag="VVL53_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU4183884.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3401..4216
                     /locus_tag="VVL53_001751"
     CDS             3401..4216
                     /locus_tag="VVL53_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4183885.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4223..4627
                     /locus_tag="VVL53_001752"
     CDS             4223..4627
                     /locus_tag="VVL53_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4183886.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4925..6091
                     /locus_tag="VVL53_001753"
     CDS             4925..6091
                     /locus_tag="VVL53_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU4183887.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPVT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6207..7244
                     /locus_tag="VVL53_001754"
     CDS             6207..7244
                     /locus_tag="VVL53_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU4183888.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7361..8574
                     /locus_tag="VVL53_001755"
                     /pseudo
     CDS             7361..8574
                     /locus_tag="VVL53_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
BASE COUNT         2500 a         1664 c         2003 g         2661 t
ORIGIN      
        1 tataaatgca ttctatacaa aattatttta ggtactatat tccaaattta aagagcgttt
       61 gaaatgggtc taaaaatgtt tgaaactaat tgaatgtgtg tataatagta aaaatgcagt
      121 aaatatcccg acaatttcta ttgacaattc aaacagattg gtttataatt aatataacaa
      181 caaatgaaag cgcaaacttt cgcggtcgga aggtagtttt atgacacgat ttgagatacg
      241 agatgatttc tatctcgatg gaaaatcatt taagatttta tctggtgcca ttcattattt
      301 tagggttcct ccagaggatt ggtatcattc gctctataac ttgaaggctc ttggttttaa
      361 tacggtagag acttatgttg cttggaattt acacgagcct cgtgaaggtg aatttcattt
      421 tgaaggtgat ctggatttag ataaatttct ccaaatagcg caggatttgg gtctctacgc
      481 aattgtgcgt ccgtctccat ttatctgtgc ggaatgggaa ttcggtggct taccagcttg
      541 gctcttgacc aagaacatgc gaattcgctc atccgaccca gcatatatcg aggcagttgg
      601 tcgctactat gatcagttat tgccaagact ggtgcctcgt ttgttggaca atggtggcaa
      661 tattctcatg atgcaggttg aaaatgagta tggttcttac ggagaagata aggcttacct
      721 gagagcgatt cgacagctaa tggaagagcg tggcgtaacc tgtcctctct ttacatcaga
      781 tggtccatgg cgagctactc tgaaagctgg aaccttaatt gaagaggacc tctttgtaac
      841 aggaaacttt ggttctaagg caccttacaa cttttcgcag atgcaggaat tctttgatga
      901 acatggtaag aaatggccac tcatgtgtat ggagttctgg gatggttggt tcaatcgctg
      961 gaaagaaccg attatcacac gggatcctaa ggaattggca gatgcagttc gagaggtttt
     1021 ggaacaaggc tctatcaatc tttacatgtt ccacggtggt acaaactttg gtttcatgaa
     1081 tggttgctca gctcgaggaa ctttggacct gccacaagtt acgtcttatg attacgatgc
     1141 ccttctggat gaagaaggaa atccaactgc taaatatctt gcagtcaaga agatgatggc
     1201 aacacatttt tcagagtatc cgcagttgga accactctac aaagagagta tggagttgga
     1261 tgctattcca ctagttgaaa aagtttcttt gtttgaaacc ttagatagct tgtcaagtcc
     1321 tgtagaaagt ctctatcctc aaaagatgga ggagctggga caaagttatg gctacctact
     1381 ttatcgaaca gaaacaaact gggatgcaga agaagaaaga cttcgtatca ttgatggtcg
     1441 agatagggcc cagctgtatg tcgatggtca gtgggttaaa actcaatatc agacagagat
     1501 tggggaagat attttttatc aaggtaaaaa gaaagggcta tctaggttag atatcttgat
     1561 agaaaatatg gggcgtgtca actatgggca taagttctta gcggatacgc aacgtaaggg
     1621 aattcggaca ggggtctgta aggatctgca tttcttacta aactggaaac actatccact
     1681 cccactagac aatcctgaga aaattgattt ttcaaaagga tggactcaag gacaaccagc
     1741 cttttacgct tatgacttta cagtcgaaga gccaaaagat acttacctag acttgtctga
     1801 gtttggtaag ggagttgcct ttgtcaatgg gcagaatcta ggacgttttt ggaacgttgg
     1861 cccaactctc tcactttata tccctcatag ctatctcaag gaaggtgcca accgtatcat
     1921 tatctttgaa acagaaggtc aatataaaga agagattcat ttaactcgta aacctacact
     1981 aaaacatata aagggggaaa acttatgaca attgtaggat gccgtattga tggacgtttg
     2041 atccacggac aagtagccaa tctttgggct ggaaaactaa atgtttcacg cattatggtt
     2101 gtagacgacg aagttgtcaa caacgatatt gaaaagagtg gtttgaaact tgcgacacca
     2161 ccaggtgtga aattgagtat tttgccagtt gagaaagctg cagccaatat tcttgctggt
     2221 aaatacgata gccaacgtct ctttatcgtg gctcgtaaac cagaccgctt ccttggtttg
     2281 gtagaagcag gtgtaccact tgaaaccctt aatgttggga atatgtctca aacaccagaa
     2341 actcgttcta ttacacgttc tatcaacgta gtagacaagg atgtggaaga cttccacaaa
     2401 ctggcagaaa aaggtgttaa acttactgct cagatggttc caaatgatcc aatttcagac
     2461 tttttgagct tattaaaata ggaaaaaaat ttttaggagg tcattgttat gatacaatgg
     2521 tggcaaattt tacttctcac tttgtactca gcttatcaaa tctgtgatga gttgacgatc
     2581 gtttcatctg caggttcccc tgtatttgct ggtttcatta ctggtttaat catgggagat
     2641 gtgactactg gtttacttat cggtggtaac ttgcaactgt tcgttcttgg ggttggtacc
     2701 ttcggtggtg cttctcgtat cgacgcaact tctggtgcgg ttcttgcgac agccttctct
     2761 gtttcacaag gaattgatgc accgcttgcc attactacaa tcgctgtacc agtagcagct
     2821 ctcttgactt acttcgacgt tcttggtcgt atgactacta ccttcttcgc tcaccgtgtg
     2881 gatgctgcaa tcgaacgctt tgactataaa ggtattgaac gcaactactt gcttggtgcg
     2941 attccgtggg ctctatctcg tgcccttcca gtcttctttg cccttgcttt tggtggtgcc
     3001 tttgtacaat cagtagtaga cttcgttgaa gcctacaaat gggttgcaga tggcttgaca
     3061 ctcgcaggac gtatgcttcc aggtcttgga tttgcaatct tgcttcgtta ccttccagtt
     3121 aaacgtaacc ttcactacct tgctatggga tttggtttga cagctatgtt gactgttctt
     3181 tactcatatg taacaggtct tggtggcgct gttgctggta tcgtaggtac tcttcctgct
     3241 gaagttgctg aaaaaattgg tttcgtgaac aacttcaaag gtttgtctat gattggtatt
     3301 tctatcgtag gtattttcct tgcagtgctt cacttcaaaa atagccaaaa agtagctgta
     3361 gcagcacctt ctacaccatc agaaagtggg gaaatcgaag atgacgaatt ctaattacaa
     3421 acttacaaaa gaagatttta atcaaatcaa caaacgtagc ttgtttactt tccaattagg
     3481 ttggaactac gaacgtatgc aagcttctgg ttacctttac atgatcttac ctcagttgcg
     3541 taaaatgtat ggtgatggaa ctcctgaatt gaaagaaatg atgaaagttc atactcaatt
     3601 cttcaatact tcgccattct tccataccat tatcgctggt tttgaccttg ccatggaaga
     3661 aaaagatggt gtaggttcaa aagacgccgt taacggtatc aagacaggtt tgatgggacc
     3721 attcgctcct cttggggata caatctttgg ttcacttgta cctgctatca tggggtcagt
     3781 cgcagcaact atggctatcg ctggccaacc ttgggggatc ttcctttgga ttgcagttgc
     3841 agtagcgtat gacatcttcc gttggaaaca gttggaattt gcttacaaag aaggggttaa
     3901 ccttatcaac aacatgcaaa gtaccttgac agctttgatt gacgctgcat ctgtacttgg
     3961 tgtcttcatg atgggtgctc ttgtagcaac agtgattaac tttgaaattt cttacaagtt
     4021 gccaatcggt gaaaagatga ttgatttcca agacatcttg aaccaaatct tcccacgttt
     4081 gcttccagca atctttactg cctttatctt ctggttgctt ggtaagaaag gtatgaactc
     4141 tactaaagct atcggtatta ttatcgtact tgctttggct ctttctgccc ttggtcactt
     4201 tgcacttgga atgtaattcc ttatgactaa atcattaatt ttggtgagcc atggtcgctt
     4261 ctgtgaggag cttaggggta gcacagaaat gattatgggt ccacaagaca acatttacac
     4321 agtagctctt cttccagaag atggcccaga agaatttact gctaaatttg aagctgttat
     4381 tgaagggttg gatgatttcc tagtctttgc ggatcttctc ggtgggacac cttgtaatgt
     4441 ggtgagtcgc ttgatcatgg aaggtcgtga tattgacctt tacgcaggga tgaatcttcc
     4501 aatggtgatt gaatttatca atgcgagcct tacaggcgca gatgcggact acaagagccg
     4561 tgctgcagaa agtattgtga aagttaatga cctgttagcg ggcttcgatg atgacgaaga
     4621 tgaataatac tcttcgaaaa tctcttcaaa ctacgtcaat gtcgccttgc cgtaggtata
     4681 tgttactgac ttcgtcagtc ttatccggca acctcaaaac ggtgttttga gctgacttcg
     4741 tcagttctat ctgcaacctc aaagcagtgc tttgagcagc ctgcggctag tttcctacag
     4801 attttagttg gaactcgatt caattcatgt gacaacgtga aaatcgttag agcattttat
     4861 atagaatata catgggaatg gagcttactc ccattcccat atttaataga aaaagaggaa
     4921 ctcaatgcta cattatacaa aagaagactt gctcgaattg ggtgcagaaa tcactacgcg
     4981 tgaaatctac caacagcctg atgtatggag agaagctttt gaattttatc aagcaaaacg
     5041 tgaagaaatt gcagcctttc tacaagaaat cgctgataaa catgactata ttaaggttat
     5101 cttgacaggt gctgggactt ctgcttatgt gggagacacc ttgctacctt actttaagga
     5161 agtctatgac gaacgcaaat ggaatttcaa tgctattgcg acaacagata tcgttgccaa
     5221 tccagtaacc tatttgaaaa aagatgtggc aactgtcctt gtgtcttttg ctcgtagtgg
     5281 gaactcgcct gaaagtgtgg cgactgttga tttggccaaa tccttggtgg atgagcttta
     5341 tcaagtgacg attacttgtg cagcagatgg taaattggct cttcaagctc acggtgatga
     5401 ccgtaatctc ttgctcttgc aaccagctgt ctctaatgat gctggatttg ccatgacttc
     5461 tagctttacg tctatgatgt tgacagctct cttggtcttt gatcctacag aatttgctgt
     5521 taagtctgaa cgttttgaag ttgtatctag tcttgcccgt aaagttttag acaaggcaga
     5581 agatgtcaaa gagctcgttg atttagactt taaccgtgtc atctatctag gcgctggtcc
     5641 tttctttgga cttgctcatg aagctcagct caagattttg gaattaactg ctggtcaagt
     5701 tgcgaccatg tatgaaagcc cagttggctt ccgtcacggt ccaaaatctc ttatcaacga
     5761 caatacagtt gttttggtct ttggtacaac gacagactac actcgtaagt acgacttgga
     5821 cttggttcgt gaagttgctg gtgaccagat tgctcgtcgt gttgtgcttt tgagtgatca
     5881 agcttttggt cttgaaaatg tcaaagaagt ggcccttggt tgtggcggtg tcttgaatga
     5941 tatttaccgt gtcttccctt acatcgttta tgcccaactc tttgctctat tgacttcact
     6001 caaggtagaa aataaaccag atacaccgtc tcctacaggt acagtaaacc gtgtagtaca
     6061 aggtgtcata attcacgaat atcaaaagta agacagcgtt tatgaattct tgacaagagg
     6121 atttgtaaat tatcagataa accatagatt gtcagtacgc tttctatggt ttgtttgctt
     6181 gagagaaata gtaaaaggag aacagaatga aagcatacac agagcgtgta tttggaaatg
     6241 ttgagggcga ggatgtcttg gcctatcgat ttgagacaga cggtggctac caacttgagg
     6301 ttatgactta tggcgcgacc atcttgcgct atgtcgcacc tgacaaggct ggaaattttg
     6361 ccaatgttat cttgggattt gatgactttg atagttatgt aggcaatagt cccaagcatg
     6421 gagcaagtgt aggtcctgta gcgggtcgta ttgcaggtgc gacctttgag ctcaatggta
     6481 agacctatga ccttgaggtt aataatgcta gcaactgtaa tcacagtggt tcaactggtt
     6541 gggattccag cttgtttgaa gttgaagaag taagcgatca tggcttgact ctctacacag
     6601 agcgtacaga tgggacagga gggttccctg gaaatctcaa gatttggatc agttatcact
     6661 tggaagaaac tggtgcctat gaaatcagct acaaggtaac gaccgatcag gatacgctgg
     6721 tcaatccaac caaccacagc tatttcaact tgtctggtga tttcacgcag acgattgacc
     6781 gtcatgtctt ccaactaaac acagagggca tttactcaat cgctcctgac ggtgttcctg
     6841 ccaaaactcc agaagccaac cgtgatgtgg tcaaacacgt ctacaatggt accttgttga
     6901 aggatatctt tgcagaagaa gatgagcaaa tccagctggc atcaggtttg gatcatccat
     6961 ttgcccttcc tgcaggccat gacaatgctg gattccttta tgaccaaaat tcaggtcgct
     7021 tcctgctttt caagacagaa gctccttgct ttgtggtcta cacagcaaac tttgtggatg
     7081 aaagtgtcat cataggaggt cagccaatgc tacagcacaa tgggattgct cttgaagcgc
     7141 aagctttacc agatgccatt cacagtgacc ttaaaggcca agtcattctt aaagctggtc
     7201 aaaccttcac cagtaagaca cgttatgaac ttgttgtgaa gtaaaagagt cattgcgcct
     7261 acttttggga gctaggaata ggtacgcaga gacaaatagt aggaaaatat gatataacta
     7321 agcgttgaaa gctatctgtt aatataatat tcaaactaca ataaggagta agaaagaaac
     7381 gaagaaaatt gtatttgcta gtgccttggc tttgaccttg gctggagcag ttttgacaaa
     7441 tgatgttttt gcgaacgaca gacttgtggc aacacaaact actgatggta aaaatgaaaa
     7501 tgtattgacc tcagaggtgc taaaaccttc tagtggcaat gttttggttg gaatcaaagg
     7561 agaatttgtg gctcctcatc aacaatctat tttggatgcc atcaatgcta tctgtaaaga
     7621 agcggctgac gaaggtttgg tagataagta tgtccctatc aaatggtcaa ctgacctaga
     7681 aaaggcagct tttgccagag ctacagaagc atctataacc atggatcata cccgtctttc
     7741 tagcaaagat ctttggagtg cctttccaac ttctaatagt ataatgggag aaaatttggc
     7801 atggaatcat gacggttttc taaaagctat tgaacaatgg cgtgctgaaa aagcagatta
     7861 tgtggagaaa aaaatagtgg ttcagacaac gggaaatctg gtcactatga gtcgctaatt
     7921 aaccctaaat ttacacacat ggggatggca gcttttaaaa atcctaacaa tcaatacaaa
     7981 gctattacaa ttgctcaaac tctaggtgat gatgcttctt cagaggaatt ggctggtaga
     8041 tatggttctg ctgttcagtg tacagaagtg actgcctcaa acctttcaac agttaaaact
     8101 aaagctacgg ttgtagaaaa accactgaaa gattttagag cgtctacgtc tgatcagtct
     8161 ggttgggtgg aatctaatgg taaatggtat ttctatgagt ctggtgatgt gaagacaggt
     8221 tgggtgaaaa cagatggtaa atggtactat ttgaatgact taggtgtcat gcagactgga
     8281 tttgtaaaat tttctggtag ctggtattac ttgagcaatt caggtgctat gtttacaggc
     8341 tggggaacag atggtagcag atggttctac tttgacggct caggagctat gaagacaggc
     8401 tggtacaagg aaaatggcac ttggtattac cttgacgaag caggtatcat gaagacaggt
     8461 tggtttaaag tcggaccaca ctggtactat gcctacggtt caggagcttt ggctgtgagc
     8521 acaacaacac cagatggtta ccgtgtaaat ggtaatggtg aatgggtaaa ctaggctcag
     8581 gccataggta aagcattcat cttacttagc aaaaagaatg aacgataaga aagaggttga
     8641 tggcgaacat tggcctcttt tgatttataa agattggatt cttgtcgcct caatttcaga
     8701 cttttctatt gtaagctaat attttatagc ccattaaaag cataagcggt aatctaattt
     8761 aaaaaatgct gtaattagtc tgaagtccac acttacttgt tgagatgtta tctctgtttt
     8821 ttatcgtt
//
LOCUS       DAVHEW010000089         8697 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVHEW010000089 DAVHEW010000000
VERSION     DAVHEW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.089"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(174..542)
                     /locus_tag="VVL53_001756"
     CDS             complement(174..542)
                     /locus_tag="VVL53_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU4183889.1"
                     /translation="MDYKVRFTEQARQDLDDIFIYYSTEFSENIAKKVITRLQQITNL
                     LIFSPEGGISFDHKIGYSLYPENMLRMIVSKQYLLFYLIHEKEVHTLRIINSRTDYLN
                     QLDHLFRTCRKLFSSQIISL"
     gene            complement(532..792)
                     /locus_tag="VVL53_001757"
     CDS             complement(532..792)
                     /locus_tag="VVL53_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEU4183890.1"
                     /translation="MSTLTRDRVYNFCVNTQLFEEAKTILESKNISLSDALNLFVEQI
                     VLEKGLTIKTADQPKAELDKGYQDIIEGRLYDADEVFSKYGL"
     gene            1117..2109
                     /locus_tag="VVL53_001758"
     CDS             1117..2109
                     /locus_tag="VVL53_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4183891.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCWINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2111..2929
                     /locus_tag="VVL53_001759"
     CDS             2111..2929
                     /locus_tag="VVL53_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU4183892.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2931..3716
                     /locus_tag="VVL53_001760"
     CDS             2931..3716
                     /locus_tag="VVL53_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183893.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3755..4033
                     /locus_tag="VVL53_001761"
     CDS             3755..4033
                     /locus_tag="VVL53_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU4183894.1"
                     /translation="MFVIEEVKDENQKKTVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4018..4215
                     /locus_tag="VVL53_001762"
     CDS             4018..4215
                     /locus_tag="VVL53_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4183895.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
     gene            complement(4596..4682)
                     /locus_tag="VVL53_001763"
                     /pseudo
     CDS             complement(4596..4682)
                     /locus_tag="VVL53_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4903..>8697
                     /locus_tag="VVL53_001764"
     CDS             4903..>8697
                     /locus_tag="VVL53_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4183896.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHEDIHSAMETSKDTEEKKATVIEEKEVVSKNPVIDTKTSNEEARIKEDSNQS
                     QGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIFN
                     GSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGK
                     NFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAFN
                     YYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYK
                     MYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGI
                     PMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDK
                     VNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIA
                     VMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVF
                     SISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
                     DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTY
                     GYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARP
                     MMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKE
                     IKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPE
                     IHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEA
                     LNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPG
                     TLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIAN
                     IYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFI
                     RGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQF
                     EPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYH
                     KVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQG
                     RNR"
BASE COUNT         2819 a         1383 c         1815 g         2680 t
ORIGIN      
        1 atgaaagctt tttatatcac tattgaaaaa tttatacgag atgatgaaag ccttaagtgt
       61 tattttacaa aggttatttc aagtcgttcc aaggtaacaa gtctagatca gattgaagct
      121 gataaaacga tacaaagaaa atattcaagt gagctaaaaa aatttattgg attttataat
      181 gagattattt gtgaggaaaa tagtttccta catgtacgaa agaggtggtc gagttggttt
      241 aggtagtcgg tgcgtgagtt gataattctc agggtatgga cttctttttc atgaatgagg
      301 taaaagagca ggtattgttt agagacaatc attctgagca tattttctgg atagagggag
      361 tatccgattt tatgatcaaa gctaataccg ccctctggtg agaagatgag taggttggta
      421 atttgttgta gacgagtgat gactttcttg gctatgtttt cactgaactc tgtggagtag
      481 tagatgaaga tatcgtctaa gtcttgtctg gcttgttctg tgaagcgtac tttatagtcc
      541 atactttgaa aagacctcgt ctgcgtcgta gagacgccct tcgatgatat cttggtagcc
      601 tttatctagt tctgctttgg gttggtcggc tgttttaatt gttagacctt tttcaaggac
      661 gatttgttct acaaagaggt tgagggcatc tgaaaggctg atgtttttgc tttctaggat
      721 ggtttttgct tcttcaaata gttgagtatt gacgcagaag ttataaactc ggtctcgtgt
      781 aagtgttgac atggcatttc tccttgtaat tatattgtat tacatatata ttacaaaaaa
      841 gatagagaaa gtcaagggct gttttagtag ggaagggtgg taggttggta atttaaacta
      901 ttaaacagaa tttttgatta aaagtattat tttatgagag aaatcctaat ttcacaatcc
      961 ataggcaaac gcttgcattt cgttttttat tggactataa taggttgata taaagccttt
     1021 tgtagtaata aaatgtagaa ggtgtagaaa gtaaggattt agaatatttg tagttaaaaa
     1081 cacaatgttg ctattcctta cgatagggag atagatatgg caatgataga agtggaacat
     1141 cttcagaaaa attttgtgaa gactgttaag gaaccgggct tgaagggggc tttgcgctcc
     1201 tttattcatc ctgaaaagca gacctttgaa gcggtcaagg atttgacctt tgaagttcca
     1261 aaagggcaga ttttaggatt tatcggggca aatggtgctg ggaagtcgac aaccattaaa
     1321 atgctgacag gaattttgaa accaacatct ggtttttgtt ggattaacgg caagattccc
     1381 caggacaatc ggcaagatta tgtcaaagat attggcgtag tctttggaca acgcacccag
     1441 ctatggtggg atttggctct gcaagagacc tacactgtct taaaagagat ttatgatgtg
     1501 ccaaactcgc tctttcataa gcgcatggac tttttgaatg aagtcttgga tttgaaggac
     1561 tttatcaagg atcccgtgcg gactctttca ctgggacaac ggatgcgggc ggatattgcg
     1621 gcctccttgc tccacaatcc caaggttctt tttttagatg agccgaccat tggtttggac
     1681 gtttcggtta aggataatat tcgtcgggca attactcaga ttaatcaaga ggaagaaact
     1741 accattcttt tgaccactca cgatttgagt gatattgagc aactttgtga tcggattttc
     1801 atgattgaca aggggcaaga gatttttgat ggaacggtga gccaactcaa ggagaccttt
     1861 ggtaagatga agactctctc ttttgaactg ctaccaggtc aaagtcatct cgtctctcac
     1921 tatgaaggtc tgtctgatat gaccattgat agacaaggaa acagcctcaa cattgaattt
     1981 gatagttctc gctaccagtc agctgacatt atcaagcaaa ccctgtctga ttttgaaatc
     2041 cgcgatttga agatggtgga tacggatatt gaggatatta tccgtcgctt ctaccgaaag
     2101 gagctctagg atgatcaaat tgtggagacg ttataaaccc tttatcaatg caggggttca
     2161 ggagttgatt acttaccgag tcaactttat tctctatcgg attggcgatg tcatgggggc
     2221 ttttgtggcc ttttatctct ggaaggctgt ctttgattct tcgcaagagt ctttgattca
     2281 gggcttcagt atggcggata tcaccctcta catcatcatg agttttgtga ccaatcttct
     2341 gactagatcc gattcgtcct ttatgattgg ggaggaggtc aaggatggct ccattatcat
     2401 gcgtttgttg cgaccagtgc attttgcggc ctcctatctt ttcaccgagc ttggttccaa
     2461 gtggttgatt tttatcagcg ttggccttcc gtttttaagt gtcattgtct tgatgaaaat
     2521 catatcgggt caaggtattg tagaggtgct aggattaact gtcctttatc tttttagctt
     2581 aacgctcgcc tatctgatta actttttctt taatatttgc tttggatttt cagcctttgt
     2641 gtttaaaaat ctttggggtt ccaacctact taagacttcc atagtggctt ttatgtcggg
     2701 gagtttgatt cccttggcat tctttccaaa ggttgtttca gatattcttt cctttttgcc
     2761 tttttcatcc ttgatttata ctccagttat gatcattgtt ggaaaatacg atgccagtca
     2821 gattcttcag gcactccttt tgcagttctt ctggctctta gtgatggtgg gattgtctca
     2881 gttgatttgg aaacgggtcc agtcctttat caccattcaa ggaggttagt atgaaaaaat
     2941 atcaacgaat gcatctgatt tttatcagac aatacatcaa acaaatcatg gaatataagg
     3001 tggattttgt ggttggtgtc ttgggagtct ttctggctca aggcttgaat ctcttgtttc
     3061 tcaatgtcat ctttcaacat attccattcc tagaaggctg gacctttcaa gagatagctt
     3121 tcatttatgg attttccttg attcccaagg gaatggacca tctctttttt gacaatctct
     3181 gggcactagg gcaacgccta gtccgaaaag gggagtttga caagtatctg actcgtccca
     3241 tcaatcctct ctttcacatc ctagttgaaa cctttcagat tgatgccttg ggtgaactct
     3301 tagtcggtgg tattttattg ggaacaacag tgaccagcat tgtttggact cttccaaaat
     3361 tcctgctttt cctagtttgt attccttttg cgaccttgat ttatacttct cttaaaatcg
     3421 caacagccag tatcgccttt tggactaagc agtcaggcgc catgatttac atcttctata
     3481 tgttcaatga ctttgctaag tatccgattt ctatttacaa ttctcttctt cgttggttga
     3541 ttagctttat cgtgcctttc gcctttacag cctactatcc agctagctat ttcttacagg
     3601 aaaaggatgt gttctttaac gtaggaggtt tgatgttgat ttctctggtt ttctttgtta
     3661 tttcccttaa actttgggat aagggcttag attcctacga aagtgcgggt tcgtaaaagc
     3721 taaagtaaga ctaaaatcaa gaaagaaact tatgatgttt gtaattgaag aagtcaagga
     3781 tgaaaatcaa aaaaagacag ttgtagctga ggttttgaag gatttgccag aatggtttgg
     3841 aatcccagaa agcacacaag cctatataga aggaaccacg acactgcaag tttggaccgc
     3901 ctatcaggag agtgatttga ctggatttgt aagcttatcc tattcgagtg aagattgtgc
     3961 agagattgat tgtctcggcg taaaaaagct tatcaaggta gaaaaattgg gagccaattg
     4021 cttgctactt tagagagtga agctcgtaaa aaagttggtt atctgcaggt caaaacagtg
     4081 gcagaaggtt ctaataaaga ttatgatcga acaaatgact tttatcgagg tcttggcttt
     4141 aaaaagttag agattttttc tcagctatgg aatccgcaaa atccttgtca gattttgatt
     4201 aaaaagcttg aataatatta cttgacatct attctcagag tgccatactg taagtgtaat
     4261 cgccgattta gcttagttgg tagagcaagg cactcgtaaa gcctaggtta taggtagata
     4321 aatgactgag gagttgaaaa aatagatagg tagaagataa ccgttaagcc ttattcttag
     4381 cggttattta tattgtttaa tagcgctgat atttcatcaa ttatgcctgt tttcgtgttt
     4441 ctggtagttg ttcaagttta ttgctactat ttttgatggt atgaatgtgc ttataatgta
     4501 tcccggttaa cgaaagtttt ggacttatac tcttagaaaa tctcttcaaa ccacgtcaac
     4561 gtcgccttgc cgtgcgtatg gttactgatt acgtcagttc tatccacaac ctcaaaacag
     4621 tgttttgagc aacctgcggc tagtttccta gtttgctctt tgattttcat tgagtactaa
     4681 tcttattttt taacgtgtgc taaaaaataa attaatgcag gttaattttt cttgaattaa
     4741 attttttaaa atgatatact gtttttatgg agagataaca attatatata aatgtactac
     4801 tctatttcct agataatact tagtaaaact ttttataaca aaggctagca aagttaaagt
     4861 ttattatcta ttggaagtta tataaatatg taaggaatta aaatgaaaaa aagtacagta
     4921 ttgtcattaa ccacagctgc agttatttta gcagcctatg cccctaatga ggtagtctta
     4981 gcagacacat ctagctctga agatgcttta agcatctctg ataaagaaaa agtagcagaa
     5041 aataaagaga aacatgaaga tatccatagt gctatggaaa cttcaaagga tactgaagaa
     5101 aagaaagcaa cagttattga ggaaaaagaa gttgttagta aaaatcctgt gatagacact
     5161 aaaactagca atgaagaagc aagaatcaaa gaagattcca atcaatccca aggagataat
     5221 gcacactcgt ctgcaaataa aggcacagaa aatcccaaaa aagaagatag acttgtctat
     5281 attgctgaat ttaaagataa agaatctgga gaaaaagcaa tcaaggaact atccagtctt
     5341 aagaatacaa aagttttata tacttatgat agaattttta acggtagtgc catagaaaca
     5401 actccagata acttggacaa aattaaacaa atagaaggta tttcatcggt tgaaagggca
     5461 caaaaagtcc aacccatgat gaatcatgcc agaaaggaaa ttggagttga ggaagctatt
     5521 gattacctaa agtctatcaa tgctccattt gggaaaaatt ttgatggtag aggtatggtc
     5581 atttcaaata tcgatactgg aacagattat agacataagg ctatgagaat cgatgatgat
     5641 gccaaagcct caatgagatt taaaaaagaa gacttaaaag gaactgataa aaattattgg
     5701 ttgagtgata aaatccctca tgcgttcaat tattataatg gtggcaaaat cactgtagaa
     5761 aaatatgatg atggaaggga ttattttgac ccacatggga tgcatattgc agggattctt
     5821 gctggaaatg atactgaaca agatatcaaa aactttaacg gcatagatgg aattgcacct
     5881 aatgcacaaa ttttctctta caaaatgtat tctgacgcag gatctgggtt tgcgggtgat
     5941 gaaacaatgt ttcatgctat tgaagattct atcaaacaca acgttgatgt tgtttcggta
     6001 tcatctggtt ttacaggaac aggtcttgta ggtgagaaat attggcaagc tattcgggca
     6061 ttaagaaaag caggcattcc aatggttgtc gctacgggta actatgcgac ttctgcttca
     6121 agttcttcat gggatttagt agcaaataat catctgaaaa tgaccgacac tggaaatgta
     6181 acacgaactg cagcacatga agatgctata gcggtcgctt ctgctaaaaa tcaaacagtt
     6241 gagtttgata aagttaacat aggtggagaa agttttaaat acagaaatat aggggccttt
     6301 ttcgataaga ataaaatcac aacaaatgaa gatggaacaa aagctcctag taaattaaaa
     6361 tttgtatata taggcaaggg gcaagaccaa gatttgatag gtttggatct taggggcaaa
     6421 attgcagtaa tggatagaat ttatacaaag gatttaaaaa atgcttttaa aaaagctatg
     6481 gataagggtg cacgcgccat tatggttgta aatactgtaa attactacaa tagagataat
     6541 tggacagagc ttccagctat gggatatgaa gcggatgaag gtactaaaag tcaagtgttt
     6601 tcaatttcag gagatgatgg tgtaaagcta tggaacatga ttaatcctaa taaaaaaact
     6661 gaagtcaaaa gaaataataa agaagatttt aaagataaat tggagcaata ctatccaatt
     6721 gatatggaaa gttttaattc caacaaaccg aatgtaggtg acgaaaaaga gattgacttt
     6781 aagtttgcac ctgacacaga caaagaactc tataaagaag atatcatcgt tccagcagga
     6841 tctacatctt gggggccaag aatagattta cttttaaaac ccgatgtttc agcacctggt
     6901 aaaaatatta aatccacgct taatgttatt aatggcaaat caacttatgg ctatatgtca
     6961 ggaactagta tggcgactcc aatcgtggca gcttctactg ttttgattag accgaaatta
     7021 aaggaaatgc ttgaaagacc tgtattgaaa aatcttaagg gagatgacaa aatagacctt
     7081 acaagtctta caaaaattgc cctacaaaat actgcgcgac ctatgatgga tgcaacttct
     7141 tggaaagaaa aaagtcaata ctttgcatca cctagacaac agggagcagg cctaattaat
     7201 gtggccaatg ctttgagaaa tgaagttgta gcaactttca aaaacactga ttctaaaggt
     7261 ttggtaaact catatggttc catttctctt aaagaaataa aaggtgataa aaaatacttt
     7321 acaatcaagc ttcacaatac atcaaacaga cctttgactt ttaaagtttc agcatcagcg
     7381 ataactacag attctttaac tgacagatta aaacttgatg aaacatataa agatgaaaaa
     7441 tctccagatg gtaagcaaat tgttccagaa attcacccag aaaaagtcaa aggagcaaat
     7501 atcacatttg agcatgatac tttcactata ggcgcaaatt ctagctttga tttgaatgcg
     7561 gttataaatg ttggagaggc caaaaacaaa aataaatttg tagaatcatt tattcatttt
     7621 gagtcagtgg aagaaatgga agctctaaac tccaacggga agaaaataaa cttccaacct
     7681 tctttgtcga tgcctctaat gggatttgct gggaattgga accacgaacc aatccttgat
     7741 aaatgggctt gggaagaagg gtcaagatca aaaacactgg gaggttatga tgatgatggt
     7801 aaaccgaaaa ttccaggaac cttaaataag ggaattggtg gagaacatgg tatagataaa
     7861 tttaatccag caggagttat acaaaataga aaagataaaa atacaacatc cctggatcaa
     7921 aatccagaat tatttgcttt caataaccaa gggatcaacg ctccatcatc aagtggttct
     7981 aagattgcta acatttatcc tttagattca aatggaaatc ctcaagatgc tcaacttgaa
     8041 agaggattaa caccttctcc acttgtatta agaagtgcag aagaaggatt gatttcaata
     8101 gtaaatacaa ataaagaggg agaaaatcaa agagacttaa aagtcatttc gagagaacac
     8161 tttattagag gaattttaaa ttctaaaagc aatgatgcaa agggaatcaa atcatctaaa
     8221 ctaaaagttt ggggtgactt gaagtgggat ggactcatct ataatcctag aggtagagaa
     8281 gaaaatgcac cagaaagtaa ggataatcaa gatcctgcta ctaagataag aggtcaattt
     8341 gaaccgattg cggaaggtca atatttctat aaatttaaat atagattaac taaagattac
     8401 ccatggcagg tttcctatat tcctgtaaaa attgataaca ccgcccctaa gattgtttcg
     8461 gttgattttt caaatcctga aaaaattaag ttgattacaa aggatactta tcataaggta
     8521 aaagatcagt ataagaatga aacgctattt gcgagagatc aaaaagaaca tcctgaaaaa
     8581 tttgacgaga ttgcgaacga agtttggtat gctggcgccg ctcttgttaa tgaagatgga
     8641 gaggttgaaa aaaatcttga agtaacttac gcaggtgagg gtcaaggaag aaataga
//
LOCUS       DAVHEW010000090         8520 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVHEW010000090 DAVHEW010000000
VERSION     DAVHEW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.090"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..941
                     /locus_tag="VVL53_001765"
                     /pseudo
     CDS             1..941
                     /locus_tag="VVL53_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            967..1611
                     /locus_tag="VVL53_001766"
     CDS             967..1611
                     /locus_tag="VVL53_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU4183897.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1653..3641)
                     /locus_tag="VVL53_001767"
     CDS             complement(1653..3641)
                     /locus_tag="VVL53_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183898.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTIHKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3643..4401)
                     /locus_tag="VVL53_001768"
     CDS             complement(3643..4401)
                     /locus_tag="VVL53_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183899.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4829..5020
                     /locus_tag="VVL53_001769"
     CDS             4829..5020
                     /locus_tag="VVL53_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183900.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5374..5646)
                     /locus_tag="VVL53_001770"
     CDS             complement(5374..5646)
                     /locus_tag="VVL53_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU4183901.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5704..6153)
                     /locus_tag="VVL53_001771"
     CDS             complement(5704..6153)
                     /locus_tag="VVL53_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU4183902.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            complement(6140..8269)
                     /locus_tag="VVL53_001772"
     CDS             complement(6140..8269)
                     /locus_tag="VVL53_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU4183903.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2579 a         1774 c         1663 g         2504 t
ORIGIN      
        1 taatcgaaag atggaaaaaa gaagggaaat caaatagaga atttgcctct ctacttggaa
       61 aagctcctca aactatccac actgaaatca agcgtaggac agtccgacaa tgtcttggaa
      121 aagggcgctt caaagaggtt tattctgccg actatgctca acagtcttat gaaaacaatc
      181 gcaagcgctt ggtcaagaaa tcaagcttga ccaaggaact aaaggaaaag attctccact
      241 atcataacca aaaatttttg cctgaaatga tggttatggc taaaggggtt aacgtgggaa
      301 tttcaaccat ttactattgg attcatcatg gaaaattggg gtaaagcaag caggatctgc
      361 tttaccctag aaaaggaaaa gcgcttaaga aacaggccag caccaacttt aaacctgctg
      421 gtcaatccat cgaacagcgg cttgaagcta tcaattttcg cttggagaat gggcattatg
      481 agattgatac agttctactt acgagagcga aaaactactg cttgcttgtc ttgacggatc
      541 gaaaaagtag acatcagatc atccgattga ttccaaataa aagtgctgat gtggtcaatc
      601 aggctctaaa actcatctta aaacaacaca agattctttc catcacggca gataatggaa
      661 cggaattcaa tcgcttgttt gatgtatttt ctgaggagca catctattat gcgcacccct
      721 atgcctcttg ggaaagggga actaatgaga atcacaacag gctcattcgt agatggttac
      781 ctaagggaat caagaaaatg actcccaaag aagtcgcatt catcgaaaag tggattaaca
      841 actatcctaa aaaatgcttg gactacaagt cacccagaga agacttctgg atggctaact
      901 tgaacttgaa atttagcgtc agaaacaaat cacgaaattg attcaaattt tgcaggtcgt
      961 ttaaatatcc tgcgtgcggg tgttcttgat gctaacgatg gaattatttc cattgctggt
     1021 gtggttatcg gagttgccag tgccacgagc aatatctgga ttatcttttt atcaggtttt
     1081 gcggctatct tagctggtgc cttttcaatg gctggtggag aatatgtatc cgtttcaact
     1141 ccaaaagata ccgaggaagc tgccgtttcg cgagaaaaac tcttgctaga ccaagatagg
     1201 gaactagcca aaaaatccct ctatgcttct tatatccaaa atggagaatt caaaacttct
     1261 gcccaactct tgactaataa gatctttctt aaaaatccac tcaaggctct ggtagaggaa
     1321 aaatatggga ttgagtatga agaatttacc aatccttggc acgctgccat ttctagcttc
     1381 gttgcctttt tccttagaag tttgcctcca atgctgtcag tgaccatttt tccaagtgat
     1441 taccgcatcc ctgctaccgt ccttattgtc ggtgtggccc ttcttctcac tggttacaca
     1501 agtgctagac ttggaaaagc cccaaccaaa acagctatga ttcggaacct tgctattggt
     1561 ctcttgacca tgggagttac cttcctgctc gaacaacttt tcagcattta aaatacaaga
     1621 aatacctcga ttttgaagtc gaggtatctt ttttacattt gcacaatctt gcgataactt
     1681 cttgaagtaa tcatgaaaat cagcacatag gcgatgagga agatagcgca gatagacaag
     1741 gtcacaatca acatcatagt cgtatccagt acaccaatca cttttaaaat caggctaagc
     1801 atatggtagg caaaggcgag atgtatgaag gcaaagagca aaggaaggaa gaaaacagtt
     1861 aaaacctgtt tgtggatggt ttgcttgatt tgcttttggt ccaaaccgac tttctgcaag
     1921 ataataaagc gttcacggtc ttcgtagcct tcagaaattt gtttgtagta gatgaccaga
     1981 acagttccga ccataaagat aatggatagg aaaataccga taaagaagac accaccaaag
     2041 agggcactca tctgagcact agcatctgct agagtgctac cataaacata gctaccttct
     2101 gtgtctaatt gagcattaaa ttgattgagg tagttttcat actcctcagc gaccttgagt
     2161 tgttcttctt cgctggcatc tacattcata ccaccgtaaa actgattata gatagccgaa
     2221 tctgggaatt gatccaaaaa ggcttgtaaa tcaggaacaa caaggtaatt gtaatcagta
     2281 gtcaagatat taaacttatt tggaacatgg ttgacaatga aatctttatt aaattcttct
     2341 ttgacagaaa attgatgatc atttagagtt agagctttct gtcctttcag tccgtcattt
     2401 ttggcaaaga gaccgacctc atttcctgat agagacagtt tttgaccagt catgttttca
     2461 taatcttttt ggtcaaatac catgaaaact gtttttggtt ggacacggtt ttgtcctttt
     2521 tcaaaaatag ttaacttggt tccttcttga tttgcaatac caaagttact gtaacgaagt
     2581 acttctttct ctttgacact ataacctttg tcacttgcaa actggctcaa gagtttgtcc
     2641 aaatcttctt tttcaacatt ttgccctgaa accccaaaat catgaggatt tagaactttt
     2701 ttaaaggatt ctgcggaatt gaaaatgctt gtcgctgctg acatggttac caaaaccatt
     2761 gttgacaaaa tagcgatggt tgctagtcca accgcatttt tcttcatacg gaaaatcaag
     2821 ttggaaacag atatgaggtt attaggttgg taatagtatt tcttgttttt ctttaagatt
     2881 tgtaggaaga ctgtaatccc tgcattaaac aatagataag taccaaagat aaccagcaaa
     2941 acagctagga agaaggttgt tagggctgta agaggatcgg ttaccgtaag ggcaagataa
     3001 tagccaatcc ctaaacttat ggaaccaaga atcgtttgga gaggaaggaa gcgacctttt
     3061 ttctctccgc tagctttctc acgtgagagc tggagggcat tcatacgggc gattcgaagg
     3121 gcattcagga acatgaggcc taggaaaatc aatccaaaga caagaagtac tgtaatgaca
     3181 actttcatct ggaaggtagc gaccagttcc accttcaatt tcatcagttt gagtaggaaa
     3241 gcgaaaatca acttgtcaaa caaggctcca ataccgattc ctgctccaac agttagaatc
     3301 ccaaatagca ctaactcctt aaaggtcata ctgattagat ggcgcttctc caagcctaac
     3361 atgccatata tacccagttc cttggaacgg tttttcatga caaaactatt ggcatagagg
     3421 acgataatgg ctgacgcaag ggtaacgaca aacataccaa atccaagtgt tgcttgaatg
     3481 gtggttcctc cacggatttc cgcaatcttt ggattgaagg ttagggagta aaagagatag
     3541 gtgatggtga ctgccaagag aacagccagt gcaaagggat agtagagttt gcggttttta
     3601 atcaagttcg ataccgctaa cttattggtt aatcgaaaca tactaattca cctcgcttgc
     3661 catgacagtc aaggtatcag agatttcttg gaacatctga cgctctgtct tctctccacg
     3721 gtagatttgg ttgtaaagaa tgccgtcttt gataaagaga acacgcttgg ccctgctagc
     3781 agctgctgtt gagtgggtta ccatgaggat ggtttgccca cgctcattga tttcattaaa
     3841 gacatcaagt aaggctgcag atgacttgga atcaagggct cctgttggct cgtccgcaag
     3901 gagaatttca ggttctgtga tgatggcgcg ggctactgct acacgctgtt tctgaccacc
     3961 agaaatctcg taagggtact tctcttgcaa ttggttaata cccagattct cagctgtcac
     4021 caccaatttc ttcatcatct ccgttatagg tcttcttgac aagacaagcg gaagcaagat
     4081 attgtcctta acagacagag tatctagcaa gttaaagtct tggaagacaa atcctagctt
     4141 ttcacgccgg aaactagaag cctgtgaatt tttaatagtt gcggtgtcag ttccattcaa
     4201 gtaaacctga ccacgacttg gtttatccaa catagctaga atattgagaa gagttgattt
     4261 accagaacca gactcaccca tgatggcaac gtagtcaccc ttttctacgg taaagtgaat
     4321 atccttgagg gcttctactt ggttgccctg aaaacgtgtt ttataaattt tttgaacgtg
     4381 ttttacatct aaaagtgtca tgaaaatctc ctttcttaat atcccatcaa atgaaatgta
     4441 gcttgcatca tagtgcatcc cagctgaacg cgctcaattt ctttgtgact tggttttcta
     4501 gttttcttat gtaagatttg tttcatgatg ttactccttt gttctttatg agtctagttt
     4561 acatcaaaaa aagaccgctt gccataacct aaccttacaa aagagacttt aatcttacat
     4621 ttttgtaaga ttgggtgaaa tataagcaaa attacttaga aaatgatttg atttcctatt
     4681 tttaaagttg taaaatataa gtgtgaaaag gcttactttg aaaaggagga agcgggaact
     4741 aacattttag taagcttacc tagataacat accattcaat ttaacacttg caagtcgttc
     4801 aacctataag gaaagaaggt gcgctttaat gaaacacaaa gaacatatac tcatcggact
     4861 tctctatctg ctctctccat tcatcggtca gctcttagtt gaacacacac actttatcag
     4921 tacagaattt accggcactg cttatgttat ttgctggtta tcagttgtta tcagcatcca
     4981 ccatttttcc aaaaatgtat tgtctcaaca gcaaaaatag attaaaaaac tgcgtttaaa
     5041 ttactgcttt tatagtaaaa tgaaataaga acaggacaaa tcgatcagga cagtcaaatc
     5101 gatttctaac aatgttttag aagtataggt gtcctattct agttccaatc cactatagtt
     5161 tgtggatgaa gctggcgaaa tattattaat actattcaaa aatcaaagtg caaactatat
     5221 caacttcaca ttgccgacta aattacagcc tgcggctggc ttcctagttt gcactttgat
     5281 ttttattgag tataaaagta aaaaagcttt aaatcaagca gtttagacta ttgatttaaa
     5341 gctttttgcg tattccaaat cttttgtaaa acctcaccaa aaccagcctt cattatcgtc
     5401 aatggcttga gcttctttgc gtttacgtgg gcctgttccg tacatttctg cttcgatttc
     5461 ttccttggtt ggcatgatag aagctaggat gatatagata atcaccccaa gtccaaagtt
     5521 tgcgactgta aaaatggcaa acagaaaacg tacaagggta acatcaaagt tccacttgtc
     5581 tgacagacct gctaaaactc ctgaaatcat gcgatttcgt ctcattttat aaaacttact
     5641 gttcatgata tttcctcttt ctgctaggtt agactagtat atcacggata ggctcggctt
     5701 tcatcagtcc tcaggctgat ttactaatag caactttcct cgacaaagtc cacagcgata
     5761 gcgttgggta tcaatcctac gcttacgctg atacctttgc tggcaggatt ggcaacgata
     5821 gagcttgatt ggcttggagt tactattggg caaggatggt acaaaccgta atccatccac
     5881 tgctttcaac agttccttaa aatcccgatc cttgtgttga tagcctttcc cttgaaaata
     5941 gaggtgataa tgacagagtt catgtcggac aattttccta aaaacgtcca accccagttc
     6001 ctgataaacc ttgggattaa aatccaaatg cccatctttg gggaaaaatc gcccacctgt
     6061 cgaacgtaga cgcctattcc actggacatg atggataaaa ggtctgccga agtcttctag
     6121 tgaaacctgc ttgacgtaat cagtcagttt catttggagc taggagagac agattaactt
     6181 tttcacgttc agtatcaatt ttcttaaccc aaacgctcac caaatctcca actgccacca
     6241 cttgactagg gtgtttgata aacttgcgac tcatatggga aatatggatg agaccgtcct
     6301 catgaattcc gatatcaaca aaagcaccga aatcaacaac gttacgcacc actccttcta
     6361 gcttttgtcc aaccactaag tccttgatat ctaggacatc ttggcgaagc acaggtgcgt
     6421 caaaggaatc acggaaatct cgacctggtt tgagaagatc tgcaatgata tctttaagag
     6481 tttctggacc aaggtctaac tcttgcgcca tttccttgac tgaaagcgac ttgagtttgc
     6541 tttgggcttc ttcgtttagg tctttaatat ctaaacgttt gaagagttcc ttaactgcag
     6601 tgtaattctc tgggtgaact cctgtattat caaggatatt gctactttca gggatacgaa
     6661 ggaaaccagc agcctgctca aaggccttgg ctcccagacg aggaactttc ttgatttggg
     6721 cgcgtgaagt gatttttcct tcttcctcgc ggtatttgac aatattttca gagatagttt
     6781 tgttgagtcc agctacgtgt gaaagaagag ctgggctagc tgtattgaca ttgacaccaa
     6841 cttggttaac cactgtatcg acaacaaagt ccagactctc agatagtttc ttctgactga
     6901 catcgtgttg gtattgaccg acaccaattg acttaggatc gattttgacc aattccgcaa
     6961 gaggatcttg caaacgacgg gcgatagaaa tggcagagcg tttttcaacg gtcaagtctg
     7021 gaaactcctg acgagcaagt tcgctggcag aatagacaga agcaccactt tcattaacga
     7081 taacatagct gacttcaggg aaatctttca gaacttccgc tacaaaagct tcactttcac
     7141 gactggccgt tccatttcca atggcaataa tctctacacc gtattgacca attaaatctg
     7201 ctaaatcttt cttggcttct tcgatttgac gagctgatgc tggtttaaca ggataaataa
     7261 cctgagttgt cagcattttt cctgttgcat ccacgacagc tagcttggca cctgtacgaa
     7321 aggctgggtc aaatccaaga accacgcgcc ctttcagtgg agcaaccaag aggagattgc
     7381 gcagattgtc agaaaaaagt tggatagctc cttcttcagc tttctcagtt aattctgtcc
     7441 gaatacgacg ctcgatagca ggcaagacct ttttcttaac ggattgctga acaacttcat
     7501 caatataagc atttttcacc ttgaaacgag tagcaaagaa ggcaagaata cggtccgtcg
     7561 catgttcaaa accgatcttc aagacaccaa gtttctcccc acgattgaga gccaaggtac
     7621 gatagccttg catagttcca actgtctctg aaaaatcata ataaatctga aaaacctgct
     7681 tttcatcaag actttcatcc ttggcttgag aagtaagttt agagtgtctc agcacttcct
     7741 gataagtcat agaacgcaag gtcacatctt ccgataaggc ttcgaccaaa atatcaactg
     7801 caccggtcaa ggcttccttg ccagtcgcaa atccttcaca gacgaacttt tcagcttctt
     7861 tctctaagtc aactatattc tgcaaaatca agcgagcaag aggaaagagt ccagcttcac
     7921 gggcaatggt tgccttggta cgacgctttt ccttataagg aagatagagt tcttcaacgt
     7981 ctgctaattt ttcggcaact aagatagctt cttccaattc cttggtcaac ttaccttgtt
     8041 cttgaatctt agctaagaca gcttccttac ggtcattgag atttgtcaga cttttatcca
     8101 aatcaataat agccttaatc gccacctcat ccagactacc agtcatgtcc ttgcgataac
     8161 gcgcgataaa gggaatagtc gccccttcag ctgtcaaact tagaacggta tcaatttgct
     8221 ttaacgtcac tcccaaatcc tgagagattt tttcatattt tttatccata aatctattat
     8281 accacaagct aaacgtttca aattaactcg tagaacattt aaaaaatatg taggaaatag
     8341 atttatatgc tacagcgcaa taacttgcac ttaaagagca ttgccacctt tttttaacca
     8401 agccatgata tcaaaagtat ttaatggatc agacataata gccagttctg gaagatgttc
     8461 ctgacctgga ataacacatt gacttttcaa atttttatat ggacgattga ctaaaattaa
//
LOCUS       DAVHEW010000091         8428 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVHEW010000091 DAVHEW010000000
VERSION     DAVHEW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.091"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..682
                     /locus_tag="VVL53_001773"
     CDS             <1..682
                     /locus_tag="VVL53_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4183904.1"
                     /translation="GEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFL
                     VFGESQTSGVVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFL
                     NDKNYFMDLKVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGI
                     LIPDDISIIGFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSP
                     KFVRRQSF"
     gene            1284..1364
                     /locus_tag="VVL53_001774"
                     /pseudo
     CDS             1284..1364
                     /locus_tag="VVL53_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2003..3364
                     /gene="trpE"
                     /locus_tag="VVL53_001775"
     CDS             2003..3364
                     /gene="trpE"
                     /locus_tag="VVL53_001775"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU4183905.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIVPHKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKTKSMTRIGE
                     LRP"
     gene            3361..3927
                     /locus_tag="VVL53_001776"
     CDS             3361..3927
                     /locus_tag="VVL53_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU4183906.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQTIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            3938..4942
                     /gene="trpD"
                     /locus_tag="VVL53_001777"
     CDS             3938..4942
                     /gene="trpD"
                     /locus_tag="VVL53_001777"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU4183907.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            4939..5706
                     /gene="trpC"
                     /locus_tag="VVL53_001778"
     CDS             4939..5706
                     /gene="trpC"
                     /locus_tag="VVL53_001778"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU4183908.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAEDVAQRIKELQIDKG"
     gene            5693..6292
                     /locus_tag="VVL53_001779"
     CDS             5693..6292
                     /locus_tag="VVL53_001779"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU4183909.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEVAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6270..7493
                     /gene="trpB"
                     /locus_tag="VVL53_001780"
     CDS             6270..7493
                     /gene="trpB"
                     /locus_tag="VVL53_001780"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU4183910.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7486..8262
                     /locus_tag="VVL53_001781"
     CDS             7486..8262
                     /locus_tag="VVL53_001781"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU4183911.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DFGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8234..8335)
                     /locus_tag="VVL53_001782"
                     /pseudo
     CDS             complement(8234..8335)
                     /locus_tag="VVL53_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         2476 a         1604 c         2037 g         2311 t
ORIGIN      
        1 tggagaggag gatacaatta gtaattcaat tatatcaagc acaggtagcc tagtagatgg
       61 tgttattgtt ttttatgaaa gtgtagatga tatcagaatt cagtatttaa agaagaacca
      121 tatgcctttt cttgttttcg gtgaatctca aacgtctgga gtagtctatg tatctaataa
      181 taattttcaa gctacttatg atatgatgaa agctgtaact gaagaaaaat ttaaaaatat
      241 gtggttgctt atggggggag aatcccatgt taataaggat cgtgaaagag gtgtgcgttc
      301 ttttctgaat gataaaaatt actttatgga tttaaaagtc atttatggct tatctacaat
      361 tgattcagtt tattcttatg caatgcagca cttgactacc caaaattatc cggacattat
      421 ttttgtttca ggagatgaaa aggttcaggg cttgattcgt gcgtgctatg agaaaggaat
      481 tcttattcca gatgatattt ctatcattgg atttgataat attcctattt ctcagtatta
      541 tactccagct ttgtctacaa ttgctcccaa ttatgttaaa ttggctaaag aaatgataga
      601 aggtgtttta gcaattatta aaggggaaag tgtcacatct gttgaagttt ctcctaaatt
      661 tgttaggaga cagagtttct agtattgttt aattcttgaa aatcctagtg gaaaatatgg
      721 agtgttctaa aataagttct gtttagcctc cattcagttt ctattgagtt ggatccaatt
      781 tctaacaagt gggacatgtt catgttcgat ctaaggaaga catggtcata ttcgaatgat
      841 attttcaaaa aaatcgtaaa aaagtcttga caaagaaaaa acaaagtatt aaaataagtt
      901 caacaaatca gaaaagtaac tatttttgat cttcagggag cctgtggtga ttgtgaatag
      961 gtggttggaa gtagtgaaag tgggctgatt ttgaaaatga atttgaaaca atgaaaattc
     1021 ggtgcgcaca ccttacagtg cagcttgttg ttaaacaagg cagagatgta aagggatagt
     1081 ccctttataa ttgaggtggc accgcgttac caacgccctc acacggaagt atattctgtg
     1141 tgtgggcttt tttctatccg tcgtttggtt tatcttttat tagggcctta agtcgctttg
     1201 atgaacttga gttctatcta cagctccttg cctactacta aaagcaaaca aaaaggctgg
     1261 actaatacga aaaaagctag taatactcaa tgaaaatcaa agagcaaact aagaaactag
     1321 ccgcaggttg ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagctcaa
     1381 aatactgttt tgaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgagg
     1441 ttgtggatag aactgacgaa gtcagctcaa aacactgttt tgaggttgca gatagaactg
     1501 acgaagtcag ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagtaacc
     1561 atacctacgg caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tataaaggtg
     1621 taccgagatg aacatctctt catcaatgtc tctggtcgtg gaggcaagga cctagctgcg
     1681 attgcagatt actagaagct aaaaaataat agatggaaaa attacagttc tttctctcac
     1741 agagagcttg tggttgctga aaacaagcag agaaagctgt aaagttgggt tcatctgaaa
     1801 cgagagaaga aaacatcatt ctcaagggag tgccctttat cgcacagcct gttacaggag
     1861 atatctgaga caggaatgag agataggaga aatcctataa ttgaggtggc accgcgaatt
     1921 tcgtcctcac gcaagttatt ttgcgtgggg atttttcata cagtcgctac gaactagaat
     1981 tagaactgaa agggaaatta caatggaacg aatcattcat ggagatgtct tatcaccaat
     2041 cttggcttat atgcgcttaa aggggcaaca taaggttatt ttagagagta ttccgagaga
     2101 caaggaaacg gctcgttttt ctatcctagc ctataatcca gtttttgaga ttaagtttga
     2161 aaatggagtt ctctatcaaa atggtcaagt gattgatcgg gatcccttgg atttccttta
     2221 tgaagtgatt cataagagtc agcaccactc agagctacct tttggtggtg gagccattgg
     2281 ttttgtgggt tacgatatga tttcgcttta tgaagaaatt ggtcaaatcc ctgaggatac
     2341 aattgggacg ccagacatgc atttctttgt ctatgagagc tatatggtct ttgaccacaa
     2401 gaaggagaag atacatgtca tagaggatgc actttatagt gagcgcagtc aagaggcctt
     2461 ggaaaaatcc ttgaaccaag tgcttgagga attacgcatt cctgctccaa atgaatttga
     2521 agacttggat ctatctccgt tagacttcaa accgcatatc gttccacata agtttgagga
     2581 aatggtggaa accgctcgtg acttgattcg taacggcgat atgttccaat gtgtactcag
     2641 ccagcgtttc tcagcagaag ttactggaaa tccatttgac ttctacagaa atctccgcgt
     2701 aactaatcct tctaattacc tttatttcta tgattttggg gattatcaaa tcatcggagc
     2761 tagtccagaa agtttggttt ctgtcaaaaa tggcatcgtg acaaccaatc cgattgcagg
     2821 gacgcgacca agaggggcta cggatgaaga agacaaggcc ttggcgactg acctcctctc
     2881 tgatgagaag gaaacagcag aacatcgaat gttggtagac ttggggcgta acgatattgg
     2941 ccgcatctct gaaacggcca gtgtccaagt tactaagtat atggaggtgg agctcttccg
     3001 ctacgtcatg catttgacca gtgtggtcaa gggacgtttg cttgcagaac tcactgccat
     3061 ggatgccttg aaagcaacgc ttcctgctgg aaccgtttct ggagcaccaa aaattcgagc
     3121 tatgagacgc atctatgaac tggaaacgga aaaacggggc gtatatgcag gagcaatcgg
     3181 ctacttgtct gcgacgggtg atatggattt ggccattgcc atccgaacca tgattctcaa
     3241 aaatcaaaga gcttatgtgc aggctggggc agggattgtc tacgactcta tcgcccaaaa
     3301 cgaataccaa gaaaccatta acaaaacaaa atctatgact agaattggag aactaagacc
     3361 atgattttat tgattgacaa ctatgattct tttacctata acttggccca atacattggg
     3421 aattttgcag aagttcaggt tctgagaaat gatgattcca agctgtatga agaagctgaa
     3481 aaagcagatg gtctggtctt ttctcctggt cctggttggc cagttgatgc tggaaagatg
     3541 gaagacatga ttcgtgattt tgcaggcaag aagccgattc tagggatttg tttgggccac
     3601 caagccattg cagaagtctt tggtggtaag ctaggtttgg ctccaaaagt catgcatggg
     3661 aaacagagca atatcaactt tgaagcgcca tctgttttgt atcaaggtat tgaggatgga
     3721 cgtgcggtca tgcgttatca cagtattttg attgaggaaa tgccagaaga ctttgaagtg
     3781 acagctcgtt cgactgatga ccaaaccatc atggggattc aacataaaaa cctaccgatt
     3841 tatggcttcc agtaccatcc agagagcatt ggaacgccag acggcttgtc ttctattcgg
     3901 aattttatcg agaaggttgt aaagtgagga aactaggatg aaagagatta ttgaaaaact
     3961 agcaaaattt gaaaatttat caggtgtgga aatgacggat gtcattgagc gtatcgtaac
     4021 tgggcgtgta actgaagcgc agattgcttc tctcctctta gctctcaaga tgaaggggga
     4081 aacacctgaa gaacgcacag ccattgccca agtcatgaga ggacatgccc agcatattcc
     4141 aactgagatt catgatgcca tggacaactg tggtacaggt ggggacaagt ctttcagttt
     4201 taatatttcc acaactgcag cctttgtctt ggctggtggc ggtattcaca tggccaagca
     4261 tggtaaccgc tcgatttctt ctaaatctgg ttccgcagat gtcctcgaag ccttgggaat
     4321 caatcttgac ctcaaaccag ctgaactagg taaggtcttt gataaaactg gaatcgtctt
     4381 tctcttcgct aaaaatatgc acccagctat gaaatacatc atgccagctc gtttggaatt
     4441 gggaattcca acgattatga acttgactgg tccactgatt catccaatgg ctttagaaac
     4501 acagcttctt ggaattagtc gtccagaact cctagaaagt acagctcagg ttttgaaaaa
     4561 tatgggtcgc aaacgtgcca tcgtggttgc tggaccagaa gggttggatg aagctggctt
     4621 gaacggaaca accaagattg cacttcttga aaatggcgaa atcagcttgt caagctttac
     4681 tccagaggat ttgggaatgg aaggctatgc tatggaagat attcgtggtg ggaatgctca
     4741 ggaaaatgca gaaattttgc ttagcgttct gaaaaacgaa gcaagtccat tcttggaaac
     4801 gacagtcttg aatgctggtc ttggtttcta tgctaatggt aagattgata gcatcaagga
     4861 aggagttgcc ttggcccgtc aagtgattgc tagaggcaag gcccttgaaa aactcagact
     4921 gttacaggag taccaaaaat gagtcaggaa tttttagcac gaatcttaga gcagaaggcg
     4981 cgtgaggtgg agcagatgaa gctggagcaa atccagcctc tgcgccagac ctatcgcttg
     5041 gcagaatttt tgaagaatca tcaggaccgc ttgcaggtaa tcgctgaggt caagaaagct
     5101 agccctagtt tgggagatat caatctcgat gtggatattg tgcaacaggc ccagacttat
     5161 gaagaaaacg gagcagtgat gatttcggtg ttgacagatg aagttttctt taaaggacat
     5221 ttggattatc tacgggagat ttccagtcag gtagagattc cgacgctcaa caaggacttt
     5281 atcatcgatg aaaagcaaat catccgcgct cgcaatgcag gtgcgacagt catcttgctc
     5341 attgtggcag ccttggcaga agaacgtctt aaggaacttt acgactacgc gacagtgctt
     5401 ggtctggaag tcttggtgga aactcacaat ctagctgaac tagaagtggc tcacagactt
     5461 ggtgcccaga ttatcggggt caataaccgc aacttgacca cctttgaggt cgacttgcag
     5521 accagtgtag acttggctaa acatttcaaa gatgattgct tgtacatttc tgaatctgct
     5581 attttcacag ggcaggatgc ggaacgacta gccccatact ttaacggaat tttagtaggg
     5641 acagctctca tgcaggcaga ggatgtggcc cagagaatca aggagttgca gattgacaaa
     5701 ggttaagatt tgtggactat cgaccaaaga agcggtggaa acagccgttt cagcaggagc
     5761 cgactacatc ggttttgtct tcgcacctag taaaagacag gtaactttag aagaagtagc
     5821 tgagttggca aagcttattc ctgcagatgt gaaaaaggtt ggagtatttg tttcaccaag
     5881 tcgggtagaa ctgctggaag cgattgacaa agttggcttg gacttggttc aagttcacgg
     5941 tcaggtggca gatgatttat ttgagaattt gccttgtgcc agtattcagg ctgtgcaggt
     6001 agatggaaat gggcatgtcc ccaattctca ggcagattat ctactctttg atgcccctgt
     6061 ggcaggaagt ggccagccct ttgattgggg tcaactggat acgactggac tagcacagcc
     6121 cttctttatc gcaggtggcc ttaatgaaga taatgtagta aaagcaattc aacattttac
     6181 tccctatgca gtagatgtat cgagcggagt ggagacagat ggacaaaaag atcatgaaaa
     6241 gattagaaga tttatagaga gggtaaagca tggcatatca ggaaccaaat aaagatggat
     6301 tttacggaaa attcggcgga cgttttgtcc cagaaacatt gatgacagca gttttggagt
     6361 tggagaaggc ctaccgtgaa agtcaggcag acccaagttt ccaagaggaa ttaaaccagc
     6421 ttttgcgcca gtacgtagga cgtgaaactc ctctttatta cgcaaaaaac ttgacccagc
     6481 atattggcgg agccaagatt tatctcaaac gggaagacct taaccataca ggagcccaca
     6541 agattaacaa tgccttagga caagtttggc ttgccaaacg catgggtaaa aagaaaatta
     6601 tcgcagaaac gggtgctggt cagcacggtg tggcaactgc aactgctgca gccctcttta
     6661 acatggaatg taccatctac atgggtgagg aagatgtcaa acgccaagcc ctcaatgtct
     6721 tccgcatgga gcttttggga gccaaggtcg aggccgtgac agatggttcg cgcgtgctca
     6781 aggatgcggt caatgcagcc cttcgttcat gggtggctaa tatcgacgat acccactata
     6841 tccttggttc tgccttgggg cctcatccat ttccagaaat cgttcgtgac ttccaaagtg
     6901 tcatcggtcg cgaagctaaa caacagtatc gcgatatgac aggtcaaaat ctgcccgatg
     6961 ccctagtagc ctgtgttggt ggtgggtcga atgccatcgg tctcttccat ccctttgtag
     7021 aagatgagtc agtagctatg tatggagctg aagcggctgg acttggtgtg gatacagagc
     7081 accacgcagc taccttgacc aagggtcgtc caggtgtcct tcacggttcc ctcatggatg
     7141 tgctccaaga tgcccatggt caaattcttg aagccttctc tatctcagcg ggtttggact
     7201 atcctggtat cggtccagag cattctcact accacgatat taaacgtgcc agctatgtcc
     7261 ctgtgacaga cgaagaagcc ttggaaggat tccaactctt gtctcgtgtg gaagggatta
     7321 tcccagcctt ggaatctagc catgctatcg cttttgcggt gaaattggcc aaagaacttg
     7381 gaccagaaaa gtctatgatt gtctgtctat caggtcgtgg ggacaaggat gtggttcaag
     7441 tcaaagaccg cttggaagca gatgcagcaa agaagggaga agctcatgcc taagacacta
     7501 acagaaaaat tgaatgctat aaaagcaact ggaaaaggaa ttttcgttcc ttatatcatg
     7561 gctggtgacc acgagaaagg tttggacggt cttgctgaaa caattcactt tttagaagat
     7621 tttggtgtct ctgcaattga agtgggtatt cccttttcag accctgttgc agatggacct
     7681 gttatcgaag aagctggctt gcgcagttta gcccacggga cctctaccca ggctttagtt
     7741 gaaaccttga aaaccattga aacagagatt ccactggtca tcatgaccta cttcaacccc
     7801 ctctttcagt acggtgtgga gaactttgtc aaagatctgg ctgatacagc tgtgaagggc
     7861 ttgattatcc cagacttgcc tcatgaacat gccaactttg tagaaccatt tttggcagat
     7921 acagatatcg ccttgattcc tttagtaagc ttgaccacag gaattgagcg ccagaaagag
     7981 ttgattgaag gggcggaggg attcatctat gccgttgcca tcaatggagt gacagggaaa
     8041 tcaggcaatt accgtgcaga tttggacaag cacttggcgc aacttcatca agtggccgac
     8101 atcccagtct tgacaggttt tggtgtatct agtcaagccg atctggaacg cttcaatgcg
     8161 gtgtcagatg gtgttattgt cggttcgaaa atagtaaaag ctctccacca aggagagccg
     8221 attcaggact ttatcaaaca agcagtagct taccaaaaat aatcaaacaa gtagcaagta
     8281 agaggaaagg cacgaaagga agtctttcct ttttcttttg caggataaag gccagtttta
     8341 tagtgaattg aatctggaat attacactac ggttcctaaa atattttcag aaagtgattt
     8401 gaatttccca atagatttgt tcagtttt
//
LOCUS       DAVHEW010000092         8392 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVHEW010000092 DAVHEW010000000
VERSION     DAVHEW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.092"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..300
                     /locus_tag="VVL53_001783"
     CDS             <1..300
                     /locus_tag="VVL53_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU4183912.1"
                     /translation="LIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLGVLKVTQH
                     NSRKTWYYVPLQDFTVNSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHVREMD"
     gene            465..3719
                     /locus_tag="VVL53_001784"
     CDS             465..3719
                     /locus_tag="VVL53_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU4183913.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(4029..5015)
                     /locus_tag="VVL53_001785"
     CDS             complement(4029..5015)
                     /locus_tag="VVL53_001785"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU4183914.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            5209..7677
                     /gene="gyrA"
                     /locus_tag="VVL53_001786"
     CDS             5209..7677
                     /gene="gyrA"
                     /locus_tag="VVL53_001786"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU4183915.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            7677..>8392
                     /gene="srtA"
                     /locus_tag="VVL53_001787"
     CDS             7677..>8392
                     /gene="srtA"
                     /locus_tag="VVL53_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU4183916.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTA"
BASE COUNT         2692 a         1449 c         1809 g         2442 t
ORIGIN      
        1 ctaatcgggc atacagatac ttttttatct attggtaatt ttaaaacaaa atttgaagcc
       61 gatgcttgta ttaaatttat taaaactaaa ttcgctagag tattattagg tgttttgaaa
      121 gttactcagc ataattcacg caaaacttgg tattacgtcc ccctccaaga ctttacggtc
      181 aattcggaca ttgattggac acaatcagtg actgatattg accgccagct tgatcaaaaa
      241 tatgaccttt cccctgaaga aattgccttt attgagaatc atgtaaggga gatggattag
      301 aaaagtattt ttatttgaca aatagtgctc aatgatctaa aatgactata taggattagg
      361 tcaggaagca tacgatgccc tgaccctttt tgtacttatg agatgagaaa gtcatttgtt
      421 agataaattg acttcgttag caaacgttca aaaaaggaaa acttatgcca gtagaaatta
      481 aaaccactaa agaaattcat cctaaaatct atgcctacac cacaccgaca gtaaccagta
      541 atgaaggctg gattaagatt gggtatacag aacgtgatgt cacacaacgt atcaaggagc
      601 aaacgcatac agctcatata gctacagatg tcttatggac tggtgatgca gcttatacag
      661 aagagcctga taaggggaaa actttcaagg accatgattt ccaccatttc ctttctttcc
      721 atgatgtaga acgtcgtccc aagacggaat ggttctattt taatggaact cctgaaaaat
      781 caaaaaatct ttttgataag tttgttcagc atgatttgtc tggttatcag cctggaaaag
      841 gacaggacta tactctgcga caagagcaag aagaagcagt tgctaagaca ttagcttatt
      901 tccaagaaca tgctggaggc aagtttctct ggaatgccaa gccacgcttt ggtaaaacct
      961 tgtctaccta tgacctagct cgacggatgg aagctgtcaa tgtcctaatt gtaacaaacc
     1021 gccctgccat tgctaactca tggtatgatg attttgaaac attcatagca ggtcaaacga
     1081 cttacaagtt tgtttctgaa tcagatagcc ttaagagtcg tccaatcttg tcacgacaag
     1141 aatttcttgg tattttagct gacgatgtaa gacaacttgc ttttatcagt ctccaagact
     1201 tgaaaggatc tgtttattta ggtggagagc acgataaact caaatgggta actgatctgc
     1261 attgggactt gttggttatt gacgaggctc atgaaggagt tgataccttc aagactgacc
     1321 aagcctttaa taagattcga cgaaatttta ctctgcattt gtcaggtaca tcatttaaag
     1381 cattggctaa aggagatttt acagaggaac aaatctacaa ctggtcttat gctgatgagc
     1441 aggctgctaa gtattcgtgg tctcttgagc aagaagagga aaatccttat gaaagcttgc
     1501 ctcagttgaa tctctttacc tatcaaatgt ctcagatgat tggcgaaaag ttagaaaaag
     1561 gcgctcagat cgatggtgaa aatattgact atgtttttga cttaagtgaa tttttcgcta
     1621 cagatgataa agggaaattt attcatgagc atgatgtcag aaattggtta gatactctat
     1681 caagcaatga aaaatatcca ttttcaacca aagaactccg taatgaactc aagcatactt
     1741 tttggctttt agaacgtgtc gcttcggcca aagcattaaa agccctacta gaagaacacc
     1801 caatctatga aaactatgag atcgttctag ctgctggtga cggacgtatg tccgaagaag
     1861 acgataaagt caaactcaaa tccttggact tggttagaaa agcgatagca gagaatgaca
     1921 aaaccattac cctatccgtt ggtcagctga cgacaggtgt cactatccct gaatggacag
     1981 gtgtattgat gttatcaaat ttgaaatcac cagctcttta tatgcaggcc gccttccgtg
     2041 ctcaaaatcc ttactcatgg agcgataaca aaggaaatca ctttcgcaaa gaaagagcct
     2101 atgtatttga ctttgcgccg gaaagaacct tgattctctt tgatgagttt gccaacaact
     2161 tatcgcttgt aactgcagct ggtagaggaa cttcagctac acgcgaagaa aatattagag
     2221 aattattaaa cttctttcca attattgccg aagaccgtgc tggtaagatg gttgaaattg
     2281 atgcaaaggc agttctaacc actcctcgcc agataaaagc tagagaagtt cttaaacgag
     2341 gttttatgtc caatctctta tttgataata ttagtggtat tttccaagca agtcaaacag
     2401 ttttagatat tttaaatgag ctgccagttg aaaaggaagg gaaggtacaa gatagttctg
     2461 atttattaga tttttcagat gttacagtcg atgatgaggg aaatgcagta gtagaccatg
     2521 aaattgtagt taatcagcaa atgcgacttt ttggtgaaaa agtttatgga cttggtgaat
     2581 ctgttgctga gttagtcaca aaagatgagg aacgaactca aaaacagctg gtcaatgact
     2641 tgagtaagac cgtttcttca gtgattgtag aggaattgaa agcagattat tctctaaaaa
     2701 caagggaaac tgagcaaatt aagaaacaaa ttacagcaac acttgagaat gaaattcgaa
     2761 aaaatgatat cgaaagaaaa atttctgaag ctcatatcaa gcaagagttg caacagcagc
     2821 tcaaagaagc aaatgataaa gcgcaaaaag ataagattca agaagatttg gaaaaacgtt
     2881 tagaagaaaa taaactcatt cataaagaaa aactagaaca aacactcaaa aaagaagtgg
     2941 aaaaaatgcc tgagaaattt atcgaacagg ttgagataaa acgtgtggaa cagttgaaac
     3001 aatcagctca agatgaaatt cgtgaccatt tacgagggtt tgcaagaaca attccaagtt
     3061 ttattatggc ttacggtgat caaactctaa cacttgataa ttttgatgcc tttgttcctg
     3121 aacatgtttt ttatgaagta acagggatta cgattgatca gtttagatat ttgcgagatg
     3181 gtgggcagga ttttgcaggg catctctttg ataaagcaac atttgacgaa gctattcaag
     3241 aatttcttcg caagaaaaag gagttggcgg attattttaa agatcaaaaa gaagacattt
     3301 ttgactatat tccaccgcag aagaccaacc aaattttcac tcctaaacga gtggtgaaaa
     3361 ggatggtaga tgatttggaa aaggaaaatc cagggatttt tgatgatcca tctaagactt
     3421 ttattgattt atatatgaag tcaggcctct atattgcaga acttgtgaag cggttatata
     3481 atagcaatgg cttgaaagag gcctttccaa atcctgaaga acgcttaaaa catattttgg
     3541 aaaagcaagt ttatggattt gctccgtctg agattatcta taacatttcc actaatttta
     3601 tatttggcaa tctttctaaa gatatcagta ggaagaattt tgttttagca gataccattc
     3661 cagcggctaa agaagggagc attcaaaagt tggttgattc ctattttgaa aataattaaa
     3721 aagaaggccg agtcaaaatt ctttgaaatc agaaaaaacg cataatattg agtgcttttg
     3781 tactgccccc caaaagttag acagaaaaaa tctaactttt ggggggcagt tcagacaatc
     3841 cttggtatta tgcgttttat tgtgggaaga tgtataatgg attgaaataa gatatgaaca
     3901 aatcaattag gaatttaaag cattttataa caacgtttta gagtaatggg gggctatttc
     3961 aacttcaacc tactataata cagaaaaaaa caactccctg ataattcaag gagttgtcta
     4021 tagttaaatt agtttttaga agcttcttgg aattctgggt ttttccatgc ttcgtcaatg
     4081 atagcttgta attctttagc agatgcttgc attttttgag tttctgcgtc gttcaatggg
     4141 atatttactg gacgaacgat accatgtgca ccaacaacag ctggttgacc gataaagaca
     4201 ttctcaactc cgtattgacc ttcttggaat actgaaagtg gaagtactgc gttttcatcg
     4261 tcaaggattg ctttagtgat acgagcaagg gctactgcga taccgtagta tgttgcacct
     4321 tttttgttga tgattgtgta ggctgcatca cgaacacctt cgaacaattc aatcaattca
     4381 gcttcttgaa cattttgagt gtctttaagg aattcttcaa ggtttacacc agcgatgtta
     4441 gcgtgtgacc aaacagcgaa ctcagagtca ccgtgttcac ccatgatgta ggcgtgcact
     4501 gaacgagcat ccacatccaa tttttcagca agtgcttgac ggaaacgagc tgagtcaagt
     4561 gaagtacctg aaccgataac gcgttcttta gggaaaccag agaatttcca agttgagtaa
     4621 gtcaaaacgt caactgggtt agcagcaaca aggaagatac ctttgaaacc agattcaaca
     4681 acttgagtta cgattgattt gttgatagca aggtttttac ctacaaggtc aagacgagtt
     4741 tcacctggtt tttgaggtgc acctgcagtg atcacaacaa ggtcagcgtc tgcacagtca
     4801 gagtattgag ctgcatagat ttttttaggt gaagtgaagg caagggcgtg actaaggtca
     4861 agcgcatcac caacagcttt ttcatgcaat tgtggaattt cgataattcc aagctcttgt
     4921 gcaattcctt ggttaacaag tgcaaaagcg taagatgaac ctacagcacc atcaccgaca
     4981 aggataactt ttttgtgttg tttagttgaa gtcattgttt taaacatctc cttaatttta
     5041 ttaggggatt ttccctagac aacttcattc tatcactttt aaaaaacttt gtcacgaata
     5101 tgccttatag ttctcgatgt aaacgtttta gtggtttaga ggctgaaata gatgggaatt
     5161 tatggtataa tgttgttact tactaattgt gaaatgaggc atttattaat gcaggataaa
     5221 aatttagtga atgtcaatct gacaaaggag atgaaggcaa gttttatcga ctacgccatg
     5281 agtgttatcg tagcgcgagc tcttcctgat gttcgagatg gcttaaaacc tgttcaccgt
     5341 cgcattctct acggaatgaa tgaattgggt gtgaccccag acaaacccca taaaaaatct
     5401 gctcgtatta caggggatgt catgggtaaa tatcacccac acggggattc ctctatttat
     5461 gaagccatgg tccgtatggc tcaatggtgg agctaccgtt acatgcttgt agatggtcat
     5521 gggaattttg gttccatgga tggagatagt gctgccgctc aacgttatac cgaggcacgt
     5581 atgagcaaga ttgctctgga aatgcttcgt gatatcaaca aaaatacagt tgatttcgtt
     5641 gataactatg atgccaatga acgggaaccc ttggtcttgc cagcgcgttt tccaaacctt
     5701 ttggttaatg gagcaactgg tatcgcggtt gggatggcaa ccaatattcc acctcataat
     5761 ctgggtgaaa ccattgatgc agtgaagttg gtcatggata atcctgaagt gactaccaag
     5821 gacttgatgg aagtcttgcc tggaccagat tttccaactg gtgctcttgt catggggaaa
     5881 tcaggtatcc ataaggctta tgaaacaggt aaaggttcga ttgtcctacg ttctcgtaca
     5941 gagattgaaa cgactaagac tggtcgtgag cgtatcgttg taacagaatt tccttacatg
     6001 gtcaataaaa ccaaggtgca tgagcatatt gttcgcttgg ttcaggaaaa acgcattgag
     6061 ggtatcacag cagtacgtga tgagtcaaac cgtgaaggtg ttcgatttgt tattgaagtc
     6121 aagcgcgacg cctcagccaa tgttattctc aataacctct tcaaaatgac ccaaatgcaa
     6181 accaattttg gtttcaatat gctcgctatc caaaatggta taccgaaaat tttgtctctt
     6241 cgtcagattt tggatgctta tatcgagcac caaaaagaag tggttgttcg tcgtacacgt
     6301 tttgataagg aaaaagcgga agcgcgcgct catatcttag aaggtctctt gattgcgcta
     6361 gaccatatcg acgaagtgat tcgtatcatc cgtgctagtg aaacggatgc ggaagctcaa
     6421 gctgagttga tgagcaagtt taagctttct gaacgtcaaa gtcaagctat ccttgatatg
     6481 cgtcttcgtc gtttgacagg tttggaacgc gataagattc aatctgagta tgatgacctc
     6541 ttggctctga ttgcggattt agcagatatt cttgctaagc ctgaacgtgt ttctcaaatt
     6601 atcaaagacg aattggatga agttaaacgt aaattttctg ataagcgccg tacagagttg
     6661 atgattggac aggtcttgag tctcgaggat gaggacttga ttgaagaatc ggatgtcttg
     6721 attacccttt ctaacagagg ctacattaag cgtttggatc aggacgagtt cactgctcaa
     6781 aaacgtgggg gtcgtggtgt ccaaggaacg ggagtgaaag atgatgactt tgttcgtgag
     6841 ttagtgtcaa ctagcaccca tgatcatctg ctcttcttca caaacaaggg acgtgtctat
     6901 cgtcttaaag gttatgaaat tcctgagtat ggtcggactg ccaaagggct accagtagtc
     6961 aatctcttga aattggatga agacgaaagt attcagacgg ttatcaatgt tgagtctgat
     7021 cgcagtgatg atgcttatct cttctttaca acccgtcacg gtattgtgaa gagaaccagt
     7081 gttaaggagt ttgccaatat tcgtcaaaat ggtctcaaag cgctgaattt aaaggatgaa
     7141 gatgagttaa tcaatgtctt gttggcagaa ggagatatgg atattatcat tggtaccaag
     7201 tttggttatg cagttcgctt taatcaatca gccgttcgtg gtatgagtcg tatcgccact
     7261 ggtgtgaaag gtgttaacct tcgtgaagga gacacagttg ttggtgccag cttgattact
     7321 gatcaagatg aggttcttat tatcacagaa aaaggatatg gtaagcgtac agtcgctact
     7381 gaatacccaa caaaaggtcg tggtggtaag ggaatgcaga cagctaaaat taccgaaaaa
     7441 aatggcttgc tggccggtct tatgactgtt caaggggatg aggatttgat gattatcact
     7501 gatacaggtg tcatgattcg aaccaatctt gccaatattt cacaaacagg acgtgcaact
     7561 atgggagtta aagtaatgcg cctggatcaa gatgctcaga tagtgacttt cacaacggtt
     7621 gcggtggcag aaaaagaaga agttgggaca gaaaacgaaa cagaaggtga agcataatgt
     7681 ctcaaaaaaa taataaaaag aaaaacaagc gaaaaaatct gctgacaaat atcctagcag
     7741 gatttctgat attactgtca ctggctttga tttttaatac tcaaattcga aatattttca
     7801 tagtctggaa taccaataag tatcaagtta gccaggtatc aaaagaaaaa ttagaagaaa
     7861 atcaggatac agaaggcaat tttgactttg attctgtcaa agctatctct tcggaagctg
     7921 ttctaacttc tcaatgggat gctcaaaaat taccagttat tgggggaatt gcaattcctg
     7981 aattggaaat gaatttgccg atttttaaag gacttgataa tgttaatctc ttctacggag
     8041 ctggtacaat gaaacgcgag caagtaatgg gagaaggaaa ttatagtcta gctagtcacc
     8101 atatctttgg tgttgataat gctaataaaa tgttattttc tcctttagat aatgctaaaa
     8161 atggcatgaa gatttatcta accgataaaa ataaagttta tacttatgaa atacgtgaag
     8221 tcaaacgtgt gacaccggat cgtgttgatg aagttgatga tagagatggg gtcaatgaaa
     8281 tcacattagt aacctgtgaa gaccttgctg ctacagaacg tattattgtc aaaggtgatt
     8341 tgaaagaaac aaaagattat tcacaaacat ctaatgaaat cctaacagct tt
//
LOCUS       DAVHEW010000093         8247 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVHEW010000093 DAVHEW010000000
VERSION     DAVHEW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.093"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            145..792
                     /locus_tag="VVL53_001788"
     CDS             145..792
                     /locus_tag="VVL53_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU4183917.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            923..1336
                     /locus_tag="VVL53_001789"
     CDS             923..1336
                     /locus_tag="VVL53_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183918.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1355..1540
                     /locus_tag="VVL53_001790"
     CDS             1355..1540
                     /locus_tag="VVL53_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183919.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(1807..2250)
                     /locus_tag="VVL53_001791"
     CDS             complement(1807..2250)
                     /locus_tag="VVL53_001791"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU4183920.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(2280..4502)
                     /locus_tag="VVL53_001792"
     CDS             complement(2280..4502)
                     /locus_tag="VVL53_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU4183921.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            4913..5155
                     /locus_tag="VVL53_001793"
     CDS             4913..5155
                     /locus_tag="VVL53_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183922.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            5495..6124
                     /locus_tag="VVL53_001794"
     CDS             5495..6124
                     /locus_tag="VVL53_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4183923.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHIVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(6247..8139)
                     /gene="pepO"
                     /locus_tag="VVL53_001795"
     CDS             complement(6247..8139)
                     /gene="pepO"
                     /locus_tag="VVL53_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU4183924.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRSEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         2240 a         1895 c         1576 g         2536 t
ORIGIN      
        1 tattttcagg gttttatttc tatcatttta ttttactttc atttggtggg agaatatttg
       61 aaaaaaagaa aggtagttta acctatgaaa aaatcactaa aaatttttgc tacatctaaa
      121 tagtttgacc tcttcggggt tgctttggtc gttgggattg cgattgcatc tggttacctc
      181 aactcacacc tcgataaatt tgtagactgg ggatcatgga cagctcttgt accctttgga
      241 ttgatttccg taaccaacgt tgggatttcc atgttgtcca ctcgtttcac ggggaaatta
      301 agcaaatggg gaaattactt tggtattgtt aataccattt tgtccggtgc tattgattat
      361 atccttggaa ataaggcggc catcattacc tatcccgtca ccttcctcat ttataccttt
      421 gctataaaga agtgggaagc ttcgcaagaa ggcagaccca accaaatgag ccaaaaacag
      481 gtaaaattgg cggccatcat catttccatc atcgccttcc tctttgcctt tgtgaccaac
      541 tatatcggat atgggggcaa gatgaatctc cttgcctacg taacaactat tgcctttgca
      601 ctgtccctca ttgccaatgc tttgaatgca ttaaaactaa caactcagtg gggcttttgg
      661 ttgatttaca atttcgttca gctgacaaaa gctggtattc aaggaaactt cgccaatatc
      721 ggtaaataca tcttttatat cctcaatgca atcggagctt tatttgtctg gaatgatgaa
      781 gaagttagat aatagaagga aactcaaata aagaatacta gccaacttaa gagaatctcc
      841 cttctgctat ttttatcatt ttagttgtaa ctcccattct tttggagtag aatgaagtta
      901 gataaaagag ggaggtcttg tcatgaaaaa actctataga atccatttta tagcaattgc
      961 agttattgat ttgctactat ttgcattttt tatcactaga ctcgagacat cttttgagtg
     1021 gctcttgctc tctggtttaa ttttcttcct tgctcaagga ctcctgctat ttctcttggt
     1081 cgtccgactc aaacatcaat tcgctgagat ttatcctcaa atcaataaaa agattcgctt
     1141 ctactattta ggggttctca ccattgattt tctatttttt gttctcttag ccttcattag
     1201 ttctcagcgt ttttcatctc ttatgccaat catcactgct tgccattcta ctttttatta
     1261 tatgacagct gactacctaa gagaaaacta tccagacttt tacgacaaac acatctcttt
     1321 atgggagtgt ctctaaagaa aaggaggttt tagcatgaaa aaaatcatct tcatcaaaac
     1381 cattcaactc cttgtcattg atggaatcat gctggcattt ttgacattta aaagggggct
     1441 tacttgggac tggattttga tttatagcgg ttggctcatt ttctttcatc ctgtgctatt
     1501 gacctatctt tcaaaccaac tttgtgacca ctttagttaa ctctattccc agattagacc
     1561 gagattctgg cgttttgctt tacaaattct cctatgggat agcctgatga ttctctcctt
     1621 ggtgtcttta agtgatattc cacttttcct tcagggaact ctcctcatcc taggacatct
     1681 catcccttcc tatcgcatct gccaaagcct gaaaagagac ttcccccaag catatcaaga
     1741 accgatttct ttttggagta ttttatgata gatgagaaag accaagccga ctgggcttgg
     1801 tctttcttat ctctttttag tatctaggat aatggtaaca ggtccattat taaccagctc
     1861 aacctgcata tctgctccaa agatgcctgt ctgaacgggc acttcttgcg ctaatttttg
     1921 attgaaagca tcatagaagt ctgatgccat atcaggttta gctgcccctg taaaggctgg
     1981 acgattgcct ctcttagtat ccgcaaagag ggtaaactga gaaatagaga ggatttctcc
     2041 ttcaatatct ttgacagaca ggttcatctt gccttctgcg tctgaaaaaa tccgcatatt
     2101 gaccagtttt ctcacagcat agtccaaatc ttcctcttgg tcctctggtc caacaccaac
     2161 cagcaataaa agtccctgat tgatttttcc ctgaatctgg ccttctatac tcacttgggc
     2221 ttttttaacc cgttggataa tgattttcat aatagccttt ctagtaagag ctaggacaac
     2281 tagccgttgg tccgtttgac agagtaaact tctggcacac tcttaatttt atcgacaacc
     2341 gtggtcagtg tagagaggtt ggcaataccg aaggacacat ggatattagc aaacttcata
     2401 tccttggttg gttgggcatt gaccgttgaa atattcttgg ttgtatttga aagaacttgc
     2461 agtacatcgt tcaacagtcc tgtacggttg agaccgtaga tatcgatatg ggccatatac
     2521 tccttatttg agctagagta ctggtcttcc cattccacat caaggagacg ttgctcgtag
     2581 ttttcttggg cacgcaggtt catacagtcc acacggtgaa tagccacacc acgacccttg
     2641 gtaatgtagc caacaatatc gtcaccaggc acggggttac aacacttagc aatccgcact
     2701 aggagaccag aagcaccttc aataaccact cccccctcat gcttgacctt gagagtttct
     2761 ttattttcaa ccttgacctc gccacctttg acaagctcct ctgcctcagc cttggccttg
     2821 gcacgctctt cctcacggcg ttccttttca gtcagacggt taaagacggt aatcgcaccg
     2881 atttccccaa aaccaatggc cgcaaagagg gagtcttctg tcttgtaact ggtcttttgc
     2941 agaacttgat ccatgtggcg cttgtccata aatttatttg ccacatagcc attttcttgg
     3001 aactgagcca tcagcatctc acgacccttg ttgacagaca attccttatc ttggttttta
     3061 aagaactggc gaatcttatt gcgcgccttg ctagtcttga ccatattgag ccagtcacgg
     3121 ctaggtccaa aggagttcgg gttggcgata atttcaacct gatcccctgt ctttaacttg
     3181 gttgtcagtg gaaccatgcg gccattgacc ttggcaccag ttgctttttc accgaccttg
     3241 gtatggattt cgtaggcaaa atcaatcggt cctgaatctt tgggaagaga acggacagct
     3301 ccatctgggg taaaaacgta aatctcctca gccagatagt tttccttaac agagtccaca
     3361 aattccttag catcatcagc ctggtcttgg agctccatca tctccttgat ccagttcatt
     3421 ccaatagctg attccttgct gttaacttgc ccctttatac ctttcttata agcccagtga
     3481 gccgcaaccc cgtactcagc cacctcgtgc atttccttgg ttcgaatctg gaattcaatc
     3541 ggcccttttg gtccataaac agtcgtatgg atagactgat aaccattggc cttgcggttg
     3601 gcaatatagt ctttgaagcg acctggcatc ggtttccaaa attcatgcac gtaaccaagc
     3661 atggcataaa catcactttg ggtatctaaa atacaacgaa tagcaatcag atcatagatt
     3721 tcctcaaacc gttttctctt gtcctgcatt ttgcggaaaa ttgagtaaat atgcttggga
     3781 cgaccataaa tcttcccttt caagtgacgt tctgtcgtat actcctctaa ttttgtgact
     3841 acctcatcca ccaaggcctc acgctccctg cgcttttcct tcatcatatg ggtaatcttg
     3901 taaaactccg ttggattgag ataacggaaa gacaagtctt ctaattccca tttgacactg
     3961 gaaatcccca aacgatgggc aagtggggca tagatttcca tggtttcttt ggaaatacgc
     4021 tcctgcttgt cttttcgaag atgtttcaga gtccgcatat tgtgcaagcg gtcagacagt
     4081 ttgaccaaaa taacgcggat gtcctcagac atggccatga gcatcttgcg atgattttcc
     4141 gctaattgct cctcgatcga tttgtactcg accttgccaa gcttggtaac tccgtcaaca
     4201 atcatccgca catcaggacc aaactctctt tccaaatcgt ccaaggtcgc atctgtatct
     4261 tccaccacat catgcaagaa tccacaagct actgttacag catccagctt tagcttagct
     4321 aaaatacctg ccacttggat agggtgaatg atataaggct cgcctgattt gcgatattga
     4381 ccactgtggc attcaacagc atagaccaag gccttatgga caaaatgaac atcctcttcc
     4441 gttaaatatt ctttggttaa agcgacaact tcttcgcctg ttaaattcac ttctttcggc
     4501 atctctactc tccaattctt cctaccattt tatcactttt ttaagaatat gaaaactaga
     4561 ttggaacaga ataagaaaaa aataattcaa aattgcttga taattctgaa ttattggccc
     4621 ataatatact acgaagttag attttaaact taggtgacag aaggagagat agaagaacgg
     4681 aaaccatatt gtaacccaaa gactttctga tttccccaat tccattgaag atacgaaaga
     4741 taaacggtgg aactcgtatc acatacactg gtaccttgac tggattttgg aattaatact
     4801 caatgaaaat caaagagcaa actaggaaac tagccgcagg ttgctcaaag cactgctttg
     4861 aggttgtaga taagactgac aaagtcagtc acatatatac ggcaaggcga cgttgacgcg
     4921 gtttgaagag atttttgaag agtataaaaa tcctcaagat actttcttct atcctttagt
     4981 ttataaggag aatacctatg aaaaaactgc tatttctatc tttgctctcc taatgttagg
     5041 agtttgctgc ctgttcttat tcagccagca aagctataaa aaactcgttc aatactatgc
     5101 taacgaccag aacctgccca gtaggataac ttatagtgaa tatagcgaca aatgagaagc
     5161 caactacggt agcactctaa acatcacgtc tatcaaacaa gctaatgacg gagtttatgc
     5221 aacctatgaa gggcaattga cacctttcca atattgataa attgataacc agcctgtctt
     5281 catctagtca tgctggtttt taagttcatt ttaaatcctt acctattctc cctaactgtg
     5341 ctatacttaa tttatactca atgaaaatca aagagcaaac tagaaagcta gccgcaggct
     5401 gttcaaagca ctgctttgag gttgcagata aagttgacgc ggtttgaaga gattttcgaa
     5461 gagtattagt acattctttg agattggagc tagtatgaaa atccataaaa ccgtgaatcc
     5521 tgttgcctat gaaaatacct attatctaga aggcgaaaag cacctcatcg tcgtcgatcc
     5581 tggtagtcat tgggaagcca ttcgtcagac aatcgagaag atcaacaaac cgatctgtgc
     5641 tattctcttg acccacgccc attatgacca tatcatgagt ctggacttgg ttcgcgagac
     5701 gtttggcaat cctcctgtct atatcgcaga gagcgaagcc agctggctct acactcctgt
     5761 cgataatctc tccggtctcc ctcgccacga tgatatggca gatgtggtca caaaacctgc
     5821 agaacacacc tttgtctttc acgaagaata ccaactagag gaatttcgtt ttaaggttct
     5881 accgacccca gggcactcta tcggtggtgt ttccctagtc tttcctgatg ctcacatagt
     5941 cttgacggga gatgctctat tccgcgaaac tatcggacgg accgaccttc cgactggtag
     6001 catggagcaa ctccttcata gtatccagac ccaactcttc accctaccaa actacgatgt
     6061 ctatccagga catggtccag ctactactat cgctcacgaa aaggccttca atcccttttt
     6121 ttagcaagat gatgacaatc gaaatttaag taaactatcc agcaaatctt tctattataa
     6181 aaggcatcct atcaaggttt tcacacgtga ttggatgcct tttttctgat gactagattt
     6241 tttgcattac caaataatca cgcgctcctc tggtgaacgc cacattccgt ctccttcttt
     6301 gacatcatat gttgtaaaga aatcgtcgaa gtttggtact tgcacattga cacggagttt
     6361 ggctggtgcg tgcacatcga cgctagccaa aagtttcata aattctgatc gacctttcat
     6421 gcgccagatg cgaccgaagt tgtagaagaa ctcttctgct gagaagtctg cttctctctt
     6481 agctgcttca agcgctgctg cgattcctcc caagtcagcc acgttttctg atacagtcaa
     6541 tttaccgtta atggttgctc cataagaatc ctgtccatca aattggtcaa tgactttttg
     6601 tgttttctcc ttgaaggcag catagtcgct ctctgtccac caatccttga ggctaccatt
     6661 ttcgtcaaag gaagctccgt tagtatcaaa ggcgtgggaa atttcatggg caatcactgc
     6721 cccaatacca ccgtagttag cagaagatga ctgatgcaag tcatagaaag gcgcctgtaa
     6781 aatggccgct ggaaagacaa tcaggttctt ctgaggattg tagtaggcat tgaccatatg
     6841 agcaggcatg ccccattcct tatagtctac aggctggttc cacttactcc aactgtgctt
     6901 gatttccaca cgcgcaaagg ctagagcatt ctcaaaaaga ctggcagttt cattcactac
     6961 cttatccttg taacgtgcag gcaattcttc tggatagcca atataaggtt tgatcacatt
     7021 gagcttcacg atagcctgtt tacaggtttc tggagtgagc cagtcattct taagcagacg
     7081 ctccttatag acatcaatca tggttgccac ttttttctcc acatccgcct tggcttctgg
     7141 agagaacttc tcacgggcgt accaaagacc cagggcttgc ttgaaaggtt cttgtgctag
     7201 atgataagct gctttgacct tatcttttgc ctctggaact ccagaaaggg cacggctgta
     7261 ggcaccagac aaaacacgga tatcctctgt taaatagctg gttgaaagat tgacaacact
     7321 caaaatcaag gttgctttaa ggagagacca ggcttcctca ctgtagaatt gctctgctgc
     7381 ttgccagaaa cgttcctcgt ctacaataac cttgtctggt aattgcccaa taactgcttt
     7441 gaagaagtca tccaaaggta gggcaggcgc gaatttcttg aaatcttcgt aagaatatgg
     7501 atgatagagt ttagcatatt ctgaactttc ttcattagag agcaccactg ccgcaactcg
     7561 gcggtccaat tcaagtcttt tttctagcaa gtcttcaatt tcttcatcag agaaatcata
     7621 agccttgagg agatttgcgc tgctttcttt ccaaagagtc aagagctctt cgcgctgagg
     7681 atgttcttct gcatagtagg tcgtatctgg caagattgtg cttggagcgc tagcccatag
     7741 aacattgatt ctagcatcca taaagtctgg cgatacacca aaaggaagga agtttggttt
     7801 tcctgcaagc tcaaactctg ctagtttagc tgtaaaatcc gcaaaagttt ccagttcttg
     7861 gaattcttta aggagtggta agacaggtgt gataccgtca gcttctctct tgtcaaaatc
     7921 acgaactagg cggtggtatt tgacaaagtt ttccaagata gcatcctcag gcacttcttc
     7981 acctgctaac cacttgtctg ttgtcgccag catcaggtct tcaatttcct ggtctaaatc
     8041 aacaaaacct cctgtttgag acttatctgc tgggatttca gctgtctgtt gccattctcc
     8101 attgatagca tcataaaaat catcttgata acgtgtcatc ttgttctcgc tttcatttgt
     8161 atttgcattt atcttaacaa aaatcactag ggaatgcaat taataaccat ttaactatta
     8221 ttttttagct attataaaaa acctttc
//
LOCUS       DAVHEW010000094         7922 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVHEW010000094 DAVHEW010000000
VERSION     DAVHEW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.094"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..1400
                     /gene="galT"
                     /locus_tag="VVL53_001796"
     CDS             <1..1400
                     /gene="galT"
                     /locus_tag="VVL53_001796"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU4183925.1"
                     /translation="KNRVLALIGEEGIDRISDENDLKQIKDYLVEIALKNGKIKDLIE
                     EKECLGAELMNFIVPLPSRLNDIFWSSYDISPQEAVEEFYKLSKDSDYIKTSAIAKNI
                     EFRASTKYGELEITINLSKPEKDPKTIAAEKLVKATNYPKCLLCMENEGYQGRINYPA
                     RSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIFLNSQHVPMAITSKTFEQLLEIVDILP
                     GYFAGSNSDLPISGGSILSHNHYQGGKHIFPMEKAKFESEFCFKDFEDVNAGIVKWPM
                     SVIRLQSENKNRLLDLATKILNKWREYSDLEVDIIAMTEDVPHHTVTPIARKVDGRYE
                     LDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPPRLKPELEEVGKY
                     LLGEDNAIADYHLEWADQLKEKYPRINKEEVNSVVQHEAGQVFARVLEDAGVYKNTPS
                     GHEAFMRFVKSVGIN"
     gene            1413..2423
                     /gene="galE"
                     /locus_tag="VVL53_001797"
     CDS             1413..2423
                     /gene="galE"
                     /locus_tag="VVL53_001797"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU4183926.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATYYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2587..3246
                     /locus_tag="VVL53_001798"
     CDS             2587..3246
                     /locus_tag="VVL53_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU4183927.1"
                     /translation="MKNLTKIKFNENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3271..4338
                     /locus_tag="VVL53_001799"
     CDS             3271..4338
                     /locus_tag="VVL53_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4183928.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4442..5452
                     /locus_tag="VVL53_001800"
     CDS             4442..5452
                     /locus_tag="VVL53_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183929.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5464..7155
                     /locus_tag="VVL53_001801"
     CDS             5464..7155
                     /locus_tag="VVL53_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU4183930.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7158..7868
                     /locus_tag="VVL53_001802"
     CDS             7158..7868
                     /locus_tag="VVL53_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU4183931.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2657 a         1120 c         1568 g         2577 t
ORIGIN      
        1 tgaaaaatag agtactagcg ttaataggtg aggagggaat tgatcgcatt tccgatgaaa
       61 atgacctgaa acaaataaaa gattacttag tggaaatcgc cttaaaaaac ggtaaaatta
      121 aagatttaat cgaggaaaag gaatgtttag gagcagagtt aatgaatttc atagttcctt
      181 tacctagtcg attaaatgat attttttgga gttcatatga tatatcacct caagaagcag
      241 ttgaagagtt ttataagtta agtaaagata gtgattatat taaaacttct gctattgcta
      301 agaatattga gtttcgagca tcaactaaat acggagagct agagattacg ataaatttgt
      361 caaaacccga aaaagatcca aaaacaattg ctgctgaaaa attagtaaaa gcaactaatt
      421 atcccaaatg tttactatgt atggaaaatg aaggatatca ggggcgaata aattatccag
      481 cacgttcgaa tcatagaatt attcggttga aacttggtga cgaagtatgg ggattccagt
      541 attctcccta ctcatacttt aatgagcatg cgatattttt gaatagtcaa catgtaccaa
      601 tggctattac ctctaaaaca tttgaacaat tattggagat tgttgatatt ttgccaggat
      661 attttgctgg ttcaaattct gatcttccta tctcaggagg ctccattctt agtcataacc
      721 actaccaggg agggaaacat attttcccaa tggaaaaagc aaaatttgag agtgaatttt
      781 gttttaaaga ctttgaggat gttaatgctg gtattgtaaa atggccaatg tcagtaatta
      841 ggttacaaag tgaaaataaa aatcgcttgt tagatttagc tactaaaatt ctaaataagt
      901 ggagagaata ttcagattta gaagtagaca tcattgctat gactgaagat gtcccacacc
      961 atacggtgac tcctattgct cgtaaagtgg acgggagata cgaattagat attgttctaa
     1021 gagataatca tactactgaa caatacccag atggtgtttt ccatcctcat caagatgtac
     1081 aacatattaa aaaggaaaat atcgggttaa tcgaagtgat gggacttgcg atattaccac
     1141 ctcgtttgaa accagagtta gaagaagttg gaaaatatct tttaggagag gataatgcaa
     1201 ttgcggatta tcatttagaa tgggccgacc aattgaaaga aaaatatcct cgcattaata
     1261 aagaagaggt taatagcgta gttcaacacg aagcaggaca agtgtttgcg agagttctgg
     1321 aagatgcagg agtttataag aatacaccgt ctgggcatga agcgtttatg agatttgtta
     1381 agtcggttgg aattaattag gaggaaaccc agatggcaat attggtaaca ggcggagctg
     1441 gttatattgg tagccatacc gtagtagaat tactaaattt aggaaaggaa gttatcattg
     1501 tcgacaacct ttcgaactct agcaaattgg tgctagaccg tattgaagca attacaggaa
     1561 tacgtcccgt gttttacgaa ttagatgttt gtgataaaca agcattgaga aaggtatttg
     1621 aacaagaatc gattgatgct gcaattcatt ttgcaggtta taaagctgtc ggagaatccg
     1681 tgcaaaagcc tgtgatgtac tacaaaaata atattatgag tacattggca cttgttgaag
     1741 tgatgtcaga atttaatgtt aaaaagattg tcttttcttc aagtgcgact gtatatggaa
     1801 ttaacaatca gtcacctcta attgagacga tgcaaacaag tgcgacaaat ccttatgggt
     1861 atacgaaagt gatgcttgag caaattttaa aagatgttca tgtggcagat tcagaatgga
     1921 gtattgcgtt gcttcgttat ttcaatccaa ttggtgctca tgagtctggt ttgattgggg
     1981 aagatccctc aggaattcct aacaacttga tgccttatat tgcacaagta gcggtaggta
     2041 agctatcaga gctaagtgtg ttcggaaatg attatgatac gcttgatggt actggtgtgc
     2101 gtgattatat ccatgtagta gatttagcaa taggacatat aaaagcttta gaaaaagtat
     2161 ctgaaaaaac agatgtttat atctataacc taggctcggg agaaggcact agtgtattac
     2221 aacttgtaaa tacatttgaa agtgttaata agatccctat tccttataaa atagttccaa
     2281 gacgttcagg agacgttgca acttattatg caaatgcaga caaagcatat aaggaattaa
     2341 attggaggac aacaaaatcg attgaagaca tgtgtagaga tacatggaat tggcaatcaa
     2401 aaaatcccaa tggctataat tgatgatact gtctatatga gtgctgtatt ttggaaaaat
     2461 tacttaacct tttaatgaaa gataaattaa aaaaaatgtt tgacagcgct tgacaaagga
     2521 aacggtttca ttgtataatg taaacacaat gacaccgatg tcaaatataa aaaagaaggt
     2581 ctttatatga aaaacttaac aaaaattaag ttcaatgaaa atggggaatt taatcatttc
     2641 cctgggaata cagttgtagc aaatctttat actaaacaag atttgatgga agttgttgat
     2701 attattcaat cacgttatag agaattacca tttatcgata agtttacttt aactccaagg
     2761 aattctattc acatgactgt aattgaattg ttgtgccatg aaaatcgtga aacggagttt
     2821 tggagcagta atcttcctct agatacacct ttacaggaaa tacatgatta ctttgctaaa
     2881 caacttgaaa tttttccatt gttggatgaa gaaattcata tgcgtataac tgaaatggga
     2941 aaacaaaaca tactagttga acctgcagat gaagcttctg caaagagatt agaagaaatt
     3001 cgtacttatg tctcagaaaa agcaggtgtt tgtttcccta atcatgatag atatcaattc
     3061 catatttcaa ttgggtatct tcggattcct ctaaccgaag aggaggaaga agagtttact
     3121 aaagtcagag cagaattaac tgaaatttta ttagagaaga ttccaactat tactgtaaac
     3181 cgtattgact atactgtatt tgaagatatg agacaatttg ttccatatca cgaaaaattt
     3241 aaataaaata ataaaaaagg agaacttatc atgaaaaaaa tgaaagtttg gtctactgta
     3301 cttgcaacgg gagttgctct tactacactt gctgcttgct ctggaggttc aaattctacg
     3361 actgcttctt catctgaaga aaaagctgat aaaagtcaag aattagttat ctattcgaac
     3421 tcagtctcaa atggtcgtgg tgattggtta actgctaaag caaaagaagc tggttttaat
     3481 ataaaaatgg ttgatatcgc tggcgctcaa ttagcagacc gtgttattgc tgagaagaat
     3541 aatgcagttg cagatatggt atttggaatt ggtgctgttg actcaaataa aattagagat
     3601 caaaaattac tagtacagta caagcctaaa tggttagata aaattgatca atctttatca
     3661 gataaagata attattataa tcctgtgatt gttcaaccat tagttttaat tggggcgcct
     3721 gatgtaaaag aaatgcctaa agattggact gaattaggta gtaagtataa aggtaaatat
     3781 tcaatttatg gtcttcaagg tggtacagga cgggcaattc tagcaagtat cttagttcgt
     3841 taccttgatg aaaaaggaga tttaggcgta tcagaaaaag gatgggaagt tgcaaaagaa
     3901 tactttgcaa atgcctatac tcttcaaaaa ggtgaaagct cagttgttaa agcgttagat
     3961 aaagaaagcc caattcaata cggtatgatc tggggttctg gagcgttaat tggacaaaaa
     4021 gaacaaaatg ttgaattcaa agttatgact cctgagattg gtgtaccatt tgtaactgaa
     4081 caaactatgg ttttaagcac tagtaaaaaa caagcgttag ctaaagaatt tattgattgg
     4141 tttggtcaat cagaaattca agtagaatat agtaaaaaat ttggatctat tcctgccaat
     4201 aaagatgccc taaaagagtt acctgaagat acgaaaaaat ttgttgatca agtgaaacca
     4261 caaaatattg actgggaagc tgttggaaag catttggatg aatgggtaga aaaagctgaa
     4321 ttagaatacg taaaataaga aataagagtt attttttatg tccaagtctc tctattattt
     4381 ctaaatttaa ggaagaaata aagagaggct tttggtttta gtaatagagg gagttagtga
     4441 gatgattaaa tttgataata ttcaaattaa atatggtgat tttgttgcaa ttgataatct
     4501 gaatttagat atacatgaag gggaattctt tacatttctt gggccttcag gatgtggtaa
     4561 atcaactact ttgagagcat tggtaggttt tttagatcca tcatcaggaa gtattgaagt
     4621 taatggaaca gatgtcactc atttggaacc tgaaaagcgt ggaattggta ttgtatttca
     4681 atcttatgcg ctatttccaa ctatgactgt ttttgataat attgcatttg gtttaaaagt
     4741 taagaaggta gctccagatg ttattaaagc taaagtatca gcagtggcag caaaaattaa
     4801 gatctctgat caacagttac agcgtaatgt atcagaatta tctgggggtc aacaacaaag
     4861 ggtagcattg gctcgtgctc tggttcttga acctaaaatt ctttgtctag atgaaccatt
     4921 gtcaaacctt gacgcaaaat tacgtgtaga tttgagaaaa gagttgaaaa gacttcaaaa
     4981 agagttaggt attactactt tatatgttac tcatgatcaa gaggaagcct tgactttatc
     5041 tgatagaatt gcagtcttta acaatggata catcgaacag gtcggtacac cagtagagat
     5101 ttatcataat tctcaaactg aatttgtatg tgatttcatt ggagatatta atgttttgac
     5161 cgatgaaaca gtccacgaag tattattgaa aaatacaagc gttttcttag aggataaaaa
     5221 aggatacatt cgattagaga aagttcgatt caatcgtgaa actgaacaag attttattct
     5281 aaaagggaca attattgatg ttgagttttc tggagttaca attcactata caataaaagt
     5341 ttctgaaagt cagattctta atgtaacaag tattgatagt caggctgcta ttagatctgt
     5401 cggagaaagt gtggaattat ttatcacacc atcagacgtt ctgcaatttt aaggaggtgc
     5461 agtatgcgtc ataaattaaa tttaaaagat tggcttattc gtttagggtt aatctggttc
     5521 ttagtaacat ttattattta tccaaacttt gatctagtag tgaatgtatt tgtaaaagga
     5581 ggagaatttt cccttgatgc tgtacatcgt gttctaaaat ctcagagggc acttcagagt
     5641 attatgaaca gttttaagtt agcattttca ctcattatta cagttaatgt cgtaggtatt
     5701 ctttgtgttc tatttacaga gtactttgat attaaaggtg ctaaaatttt aaaattaggt
     5761 tatatgacct ctttaattta tggaggagtg gttttagcga ctggatataa atttgtctat
     5821 ggtccttatg gattgattac aaaattttta caaaatgtta tcccttcttt agactctaac
     5881 tggtttattg ggtatggtgc agtcttattc attatgacat tttcaggaac tgctaatcat
     5941 acattgtttt taacaaatac aattcgaagc gttgactatc acactattga ggctgctcga
     6001 aatatgggag caaaaccatt tactgttttc cgaaaagtag tgttaccaac cttaattcca
     6061 actctatttg cacttactat tatggttttt cttagtggtt tatctgcagt agcagcaccc
     6121 atgattgttg gtggtaaaga atttcaaact ataaatccaa tgattattac atttgcaggg
     6181 atggggaatt ctcgtgattt agctgcctta cttgcaatta ttttaggtat tgcaactaca
     6241 attttgctta ctatcatgaa taagatagaa aaaggtggaa attatatttc tatctctaag
     6301 actaaagcgc ctcttaaaaa acaaaaaatt gcgtctaagc cttggaatat cattgctcac
     6361 attgtagcat atggattgtt cacagttttc atgcttccac taatttttat agtattatac
     6421 tcatttacag atccagttgc aattcaaaca ggtaacttaa cattatcaaa ctttacttta
     6481 gaaaattatc gcttattctt tagtaatagt gcggcattct ctccattctt ggtcagcttt
     6541 atttattcta ttattgctgc gacaacagca acaattctcg cagttgtatt tgctcgtgtt
     6601 gtcagaaaac ataaatctcg ttttgatttc ttatttgaat atggtgctct acttccttgg
     6661 ttactaccaa gtacactttt agcagtaagt ttattattta cttttaatca gccacaattt
     6721 cttgtcttga atcagatttt ggtaggtagt ttggtaattc tacttattgc atatatagtt
     6781 gtaaaaatcc cattttctta tagaatggta cgtgctattt tatttagtgt tgatgatgag
     6841 atggaagatg cagcaagaag tatgggtgct tcaccttttt atactatgat gaaggttatc
     6901 attccattta ttttaccagt tgttctctct gttattgctt taaactttaa ctctttatta
     6961 actgacttcg acttatctgt attcctttac catcccctag ctcaaccatt aggtattacg
     7021 attcgatctg caggtgatga aacagcaaca tctaatgcac aagctctggt atttgtttat
     7081 acaattgttc tgatgattat ttctggaacg gtattatact tcacacaaag accggggcgt
     7141 aaagtaagga aataatcatg acagccacta gtcttgggtt atcaaatatt gaaatagttg
     7201 tcaggattgt tttatcagta gtcattggta gtataattgg tttagagaga gggagcaaat
     7261 cccagcctgc aggcatccga acttatagta ttgtttgtct agctgcatgt ttgattatga
     7321 tgacgaatga atacgtatct tataaatttg ggacaggaga tcctacacga ttaggagctc
     7381 aagttatatc aggtgtgggt tttctaggcg ctggaacgat tcttattaca gataaaaaga
     7441 aaattacagg tctgacaact gcagcaggca tttgggcttc ggcaggaatt ggattagcta
     7501 ttggagtagg tttttatgag ggagctcttt tagtagccat ttctgtttgg ggtgtgatat
     7561 ccatgttcca accactaaaa aaatatctgc aaaatcgttc taaaatgatt gaattgtata
     7621 tagtggttaa atctacagaa gcctataatc gtgtattggt ttattgtgca gaaaatggta
     7681 tcagaatgac cgattcaaga acagcgtttg gagatgttaa ttctgacaga attgaatact
     7741 ttgatgttcc ggacaaaaaa atagcgtcat ttattactct ggaattatca ggtagatttg
     7801 aacatcttcg actgatggaa gaaattgcta atattgtagg tgttatttat gtcgaagaaa
     7861 ttagctgaaa tttagtaaac aaagggacag atgtttttgt ccttttgttt tttagatgtt
     7921 tt
//
LOCUS       DAVHEW010000095         7805 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVHEW010000095 DAVHEW010000000
VERSION     DAVHEW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.095"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            266..1075
                     /locus_tag="VVL53_001803"
     CDS             266..1075
                     /locus_tag="VVL53_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4183932.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYVIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKLRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(1256..2131)
                     /gene="aguB"
                     /locus_tag="VVL53_001804"
     CDS             complement(1256..2131)
                     /gene="aguB"
                     /locus_tag="VVL53_001804"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU4183933.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(2141..3226)
                     /gene="aguA"
                     /locus_tag="VVL53_001805"
     CDS             complement(2141..3226)
                     /gene="aguA"
                     /locus_tag="VVL53_001805"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU4183934.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(3223..4350)
                     /gene="nspC"
                     /locus_tag="VVL53_001806"
     CDS             complement(3223..4350)
                     /gene="nspC"
                     /locus_tag="VVL53_001806"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU4183935.1"
                     /translation="MKLEQVPTPTYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(4350..5609)
                     /locus_tag="VVL53_001807"
     CDS             complement(4350..5609)
                     /locus_tag="VVL53_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU4183936.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(5606..6466)
                     /gene="speE"
                     /locus_tag="VVL53_001808"
     CDS             complement(5606..6466)
                     /gene="speE"
                     /locus_tag="VVL53_001808"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU4183937.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            complement(6497..6634)
                     /locus_tag="VVL53_001809"
     CDS             complement(6497..6634)
                     /locus_tag="VVL53_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183938.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            6721..7738
                     /locus_tag="VVL53_001810"
                     /pseudo
     CDS             6721..7738
                     /locus_tag="VVL53_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         2263 a         1744 c         1518 g         2280 t
ORIGIN      
        1 tttcctccat tattcttcag attagttgag taatctaata ctcataccta cttacaaaaa
       61 aactattata ttaagttggt tttttaattt gtctagttgc aacattgaca aacataatat
      121 agcatatctt tgcgaaatat cctcttcaaa tcatgaattg tcatcaaaac atcttaaact
      181 ataaaatcaa ttagtctcaa gctttctatc aatttcttct ccaaaatatg ctataataat
      241 agcaaaagat aaagaaggaa gacctatgat taaactacta gccttggata tggacggcac
      301 cctcctcaat gaagccaagg aaatcccaca agctcacatt actgctattc acaaagccat
      361 tgaaaaaggt gtcaaactgg ttctctgtac gggtcgccct cttttcggtg tcctccccta
      421 ctacaaaaaa ctggagcttg acctccagaa tgagtatgtg attgttaaca acggttgttc
      481 aactcaccag accagtgatt ggagtctagt tgactggaaa gaactgagtc cagctgacat
      541 cgaatacctc tatgaccttg ctgaaaagag tgatgttcag ttgacacttt ttgacgagtc
      601 acattatttt gttctcggtg gcaaacccaa tcaagttatt gaaaatgatg ctaaactagt
      661 attttcagac ctgactgaaa tttctcttga agaagcgact agtggaaagt tacggatgtt
      721 ccaaggtatg tttttaggaa caaaagaaca aacagacgat tttgagcagc gttttgctga
      781 agagctttgc caacgattca gtggagttcg ttcgcagcct gtcatttatg aagcaatgcc
      841 gcttggaacg acaaaggcta ctgctctttc acgactagct gagattttga agattgattc
      901 ctcagagatt atggccatgg gcgatgctaa taacgatatc gaaatgctcc agtttgcagg
      961 gcttgggatt gcaatgggaa atgccagcga ttatgtcaaa tctcttgcgg atgccgttac
     1021 ctcaagcaac gaagaagacg gcgttgcgcg tgctattgag aaatatattc tataaaaaag
     1081 aaaagcaaat agacagaagt tactgctatt tgcttttttg ctaatatttt aaaatacgga
     1141 ctaatcaata gacaagaata gcgaatcata aaaatcgatt tactagatgc catagtatct
     1201 tatagttgct aataagaagt tagtatagca gaatgaatct ctcatttctc ccacactaat
     1261 ctgtaatttg tctatacatt tctggtcttc tatctcgaaa caagccccag tttaggcgtt
     1321 cacttgctcc cttgtctagg tcataagtag ctaacagaac agcttcttct tgtctttcag
     1381 ctcgttctag aatagctcct gtttcatccg tcataaagga ggaaccgtag aagtcaagac
     1441 tggaactctg tccgccattt tcctcactag gagtaacctc ctctaaacca taacgattgg
     1501 ctgcgatgac tggaacaata ttcgctgctg cgtgcccttg catagtacgt tgccagtgac
     1561 cacaactatc tgtatccaaa attggctctg aaccgatagc tgtaggataa aagagcaatt
     1621 cagcaccatt caatgcaaga cagcgcgctg tttcagggaa ccattgatcc caacagatac
     1681 cgataccaat cttagcatag cgagtattcc agaccttgaa accagtgtta ccaggcgtga
     1741 aatagaattt ttcttgataa taatggtcat ctggtatatg ggtctttcga taaacgccca
     1801 gcacttcccc atctgcatca atgacggcaa tagagttata caagacatta ccatcttttt
     1861 catagaaact gattggtaaa acaacttgta gttccttagc aatcacctta aaatgctgaa
     1921 tggcagtatt ttccgctaca gattgggcat actggtagta gtcatactga cgttcctgac
     1981 agaaataggg atgttcaaac aactcgggca agagaataat ttgggctcct tgctcagcag
     2041 cctgacgtac taaacgctct gcggtttgga tatttgttgc cacatcctta gcgcattgca
     2101 tctgaatggt tgcaactctt acatttctca tctttttctc ctattctgga atttgttggg
     2161 tgatacagtg gatattgcca ccacctaaga gaatatctct ggctggtatt ccgacaactt
     2221 tacggtctgg gaaacacttg ctgaggatat ctaaggccac ttggtcgttt acatcctcaa
     2281 actgtggaac caagacagcc ttgttggcga tataaaagtt tacgtaggaa gctgctagtc
     2341 gttcacctgc gtatcgcttt tcttctcctt cttcatagat gtagcctggc aaatcttctt
     2401 ctgtcacaac ttgtcgaact gcagggatag gcaatttatg aatggtgaag tgacaacctt
     2461 ttgcatctgt ttcctgttct aagagttcga gatctgcttt tgacatggca tactggggat
     2521 cattttcgtc atctgtccaa gccaaaacaa gctcagcagg accaacaaag gcagcaacat
     2581 tatcgacgtg ttcattggtt tcatcctgat aaataccata aggaagccaa ataacttttt
     2641 cagcaccaag actttctaat aatgtgtttt caatctcctc tttagtcaag ttaggattgc
     2701 gaccaggact aagcaagcaa ctttcagtta cgagaatagt tccttgacca tcgctatgga
     2761 ttgcgcctcc ttccagtaca aaaggtttag catcatagac aggcctttcc aaggcctcag
     2821 caaaacgact ggctacttgg tcatcctctt cataatcttg ataaagacca tcataggtgc
     2881 ctccccaagc attgaaggcc caatccacgg ctaatttctt acctttatca ttgacgagaa
     2941 tggttgggcc agtatcacgc gcccaggcat cattggtggg aatgtctaaa taaacaacct
     3001 tgtccccaag ataggattgg gcttcagata gataggcctg ctccaccaaa agatagactc
     3061 tttccccttc tgctatggtc tcgatgatct gagtaaatgc tcttttagca gcctttcctt
     3121 gaaaaggcca tgatcctggt cgagtcggcc atatcatgag ggtaccatga tggggttcgt
     3181 actctgctgg catgtgatag cctaattttt ttggactgtc catcatgata atctcccttt
     3241 aaagtcttga tagccaaaag ctttgagtaa gctacagtct ccctgttcgt ccatgagata
     3301 gagacttggc aatccaatac cattaaaggt attatttttg acaaaagaat aaatggccat
     3361 gtcttgaaaa taaagtctgt ctccgatttg gactggattt tcaaaactat aatcaccaat
     3421 cacatcgcca gtcagacagg tattagaaga aagtctgtag gtatgggctt tttcctgtgc
     3481 ctcaaagcca tttctcaaag gtggacgata gggcatctca agtacatcag gcatatggca
     3541 ggtcgcagag gcgtctaaaa ccaagatttc cataccgttt tctacaatat ctaatacctc
     3601 agttgctaaa taacccgcat taagcgcaat ggcttcacca ggctcgatat agatttcaag
     3661 attgtaagtt tttcggatac gcttgatttc tgaaatcagc aaatccacat cgtaaccttc
     3721 tcttgtaata tgatgaccac cacccatatt gagccatttt acctcatgta agtagggacc
     3781 aaactgttct tctactgctt tcaaagttgt ttgtaaatca tctgctccct gctcgcaaag
     3841 ggtatgaaaa tgaagtccgt caaccaaatc tagcaaatca ctcggaatct tgtctatagt
     3901 aactccaaag cgagaacctg gtgcacaagg gtcatagagc gcgtgatctc cttgagttga
     3961 acactgaggg ttgaggcgca aaccaacact gacaccagcc tctcgacaac gagccccatg
     4021 cttacgcaac tgtctctctg agttaaagac gatatggtct gttatcccaa gcaattcctc
     4081 caagtctgca tccttgaaag caggcgcaaa tacatggact tcaccaggaa attcttccct
     4141 tgccaatttg gcctcataga gtccactagc tgtcgtacct gatagatact ggctaatcaa
     4201 gggataagtt ttgtagaggg aatatgcctt ctgggcaagc aagaccttgc aaccggcctc
     4261 ttcttgtaca tattgtagaa tgcggcaatt agcttctaac ttggccaagt caataacata
     4321 ggttggtgtt ggtacttgtt ctaacttcat tagtccacca tttgtggatt ttcaaccaca
     4381 acccatggca aaccatactc attcaaagct tccatgaatg gatctggatc taactcctca
     4441 aggttataca ctccagcttg tttccaagtt ccgttcatga ctaattttgt cccaatcatg
     4501 gctggaacgc ctgtcgtgta tgaaattgct tgtgacccga cttctgcata gcactcttga
     4561 tggtcgcaaa cattgtagat gtagatagtc ttttcaacgc catctttgac acctgtaaag
     4621 atacatccaa tattggtttt acctacagta cgtgggccaa ggctagcagg atctggaagc
     4681 aaggctttca aaaattgaat tggaacaatt tcttggccgt taaagttaat ggtatccgta
     4741 cgaaggagtc caacattttc aagacatttc atgtgcgtca agtaagattg accaaaagtc
     4801 ataaagaaac gaatgcgttt gacacctgga atgttcttgg ccaatgattc gatttcttca
     4861 tggtgaagga gatacatatc tttttgtcca acttgaggga aatcatactc acgcttgata
     4921 gacatagctt cgacttcgac ccatttccca tcttcccagt aagaacctgg cgcagaaacc
     4981 tcacggagat taatttctgg attaaagttg gttgcaaatg gataaccgtg gtcaccgcca
     5041 ttacagtcta aaatgtcgat ataatggatt tcatcaaaat agtgtttgag ggcataagct
     5101 gaaaagacac tagttacacc tgggtcaaaa ccagaaccaa gaagagcagt caagcctgct
     5161 tctttgaatt tctcttgata agcccactgc catgagtagt caaagtaggc tgtaaaacca
     5221 agttccttac aacgtttttc gtagatagca cgccactcag ggtcttctgt gtcttctgct
     5281 tcgtagttgg ctgtatcgat atagtgaaca cctgttgcca aacaagcatc cataatggtt
     5341 aaatcttgat aaggcagagc tacattcaaa acagcttctg gtttgtagct ttcaatcaag
     5401 gtaatgacct cttctacctt gtcagcatca agtgctgcag tttcaatttt agtacttgtt
     5461 ttgccttcta gcttcgcttt caagtcatcg cattttgact tggtacggct agcaatcata
     5521 atctctgtaa acgtttcgct atcttgacaa atctttgaaa tagcaacttg ggcaacgccc
     5581 ccacaaccaa taactagtaa acgactcatt tttttccttc ctcttcttct aaaatgtcct
     5641 caacatactt gggcaacata aaggctccca cgtgtaagtt tgcagtgtag tattctgtga
     5701 aaagctggcg ttttttccag ccttccttgt caaaatcttt gacagggtgg tattttttcg
     5761 atgcaaatcc aaacaaccaa tagccagctg ggctagttgg aatatgggcc tgataaaccc
     5821 gactgattgg aaaggcttga ttgaccttgc ggtgcatgct tcggcaggcc gactcatcct
     5881 cgtcaaagaa gggactccca tgctggtaaa tcatgatgcc gtcttccttc agagctcgat
     5941 aactattgcc gtagaattcc ttggtaaaga gtccttccgt atggccaaat ggatctgtcg
     6001 catcgttgat gataatatca tagtcatctt cgcagtttcg caaaaagcgt agcccatttt
     6061 ggtagtaaat agtaacacga ggatcatcta ggcctgcagc aaagtctggg aaatactcac
     6121 gacagacctc gaccaacatc tcatccggtt ccacgatatc gatttgttcc agttcaggat
     6181 agagtgttaa tacttgggca acaccgccgt caccaccccc aataaccaat actttctttg
     6241 gatttgggtg gacagccatg ggaacgtgga cggtcatttc attgtagacg aaatcatccg
     6301 catctgagaa caagacatgg ccatttaaaa tcagtatttt cccaaaagct ggcgtatcca
     6361 agacttcgat atcctgccat tcactttttc cagcgtaaag ttgcttggct gttctcagag
     6421 acaatttgac atctggagta tgaacttcag aaaaccataa atccatctat ttctccctat
     6481 attatattta gcgcagttag ctactgccaa agcccaagtg gtatacttgg aataagccac
     6541 tatggattag ttcatttcct ccaatcgctt gacgatttcc gaaaggctct aaccacagct
     6601 tttgtttcaa ttggtaatga gtttgataac gcatagacgt ttgatctcgt ctaaggtaac
     6661 aaggcaaaaa attcgtggat aaccaagtag acttgaaata ctatatctgg aggtactccc
     6721 atgctttatg taggcattga tatcgctaaa aataaacacg atgttacagc cttgaatgtt
     6781 ccaggaaaaa ctgttcttaa accactcact ttttcaaata ataaagctgg ttttgaactc
     6841 ttagatctgt ctattcgaca gatcaaccaa gactgtctca tcgctcttga agatactgga
     6901 cattatgcct ttaatctact gaacttcctg catgaacaag gatataaagt ttacacctac
     6961 aatcctttgc tcatcaagga atttgccaaa tcattatcac ttcgcaagac taaaacggat
     7021 aagaaagacg ctcatggcat cgctcttaaa cttctttctg accccaatcg tgaacaattt
     7081 caacacgata atcggcaagt agacctaaaa atactggcta gacatattca tcgtctcaag
     7141 aaaaacagtc tgattggaaa gtacaataca ctcgttgtct tgatatcatc tttcctgagt
     7201 tggataaaat cgttggaaag cattcagaat atacctacca actcttgaca cgctacccta
     7261 atcctcagaa aaggattgag gcaggatttg ataagctgat agaaattaag cgattgaccg
     7321 cttctaaaat tcaggatatc ctctcagtcg cacctcgttc tatcggaaca acatctcctg
     7381 ctcgtgaatt cgaaatcatc aagcattaca agaggctcat tgacaaggcg gaaacatgta
     7441 tcaatgactc gatggctgag ttcaactcgg tcatcacgac ggttactggg attggggatc
     7501 gtttagggcg gtcattttag ccgagattcg aaatattcat gcctttgata atcctgctca
     7561 attacaagct ttcgctggac tggattcttc tatttatcag tcaggtcaga ttgatttagc
     7621 tggaagaatg gtcaaacggg gttcccctca tctgcggtgg gcactcatac aagctgccaa
     7681 agcatgccct cgcttttcac ctgcttctaa ggcctatctt aagactaagt tagaataagg
     7741 aaaacattac aatgtagcca tcatccacct tgcaaaaaaa cttatccgaa ccctgtttta
     7801 tatcc
//
LOCUS       DAVHEW010000096         7669 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVHEW010000096 DAVHEW010000000
VERSION     DAVHEW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.096"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            152..451
                     /locus_tag="VVL53_001811"
                     /pseudo
     CDS             152..451
                     /locus_tag="VVL53_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            522..2543
                     /locus_tag="VVL53_001812"
     CDS             522..2543
                     /locus_tag="VVL53_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4183939.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMALTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2545..3186
                     /locus_tag="VVL53_001813"
     CDS             2545..3186
                     /locus_tag="VVL53_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183940.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3290..4642
                     /locus_tag="VVL53_001814"
     CDS             3290..4642
                     /locus_tag="VVL53_001814"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU4183941.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4644..5702
                     /locus_tag="VVL53_001815"
     CDS             4644..5702
                     /locus_tag="VVL53_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU4183942.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKCGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5712..6911
                     /gene="divIB"
                     /locus_tag="VVL53_001816"
     CDS             5712..6911
                     /gene="divIB"
                     /locus_tag="VVL53_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU4183943.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLFVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
BASE COUNT         2373 a         1331 c         1673 g         2292 t
ORIGIN      
        1 aaatatctaa aaaattcgat tttttccttg acaacaaata ataaaaaagt tattcttatc
       61 acataagaaa gcggtttcaa taaaagagag aggaggattt attttgttgc attgttgctc
      121 aaggtgtgct ttttttgaaa gaaaaaataa aatgaaaacg aaaaaacata gattacttgc
      181 tctagctctt atttcaagtt ttacattatt gggagctgca tcagctgctg tataatatcc
      241 agatggagga gtatggacat atggagaagg ttcaggaggt ggttaggctt tttcaaatta
      301 ctatcatggt aaaaaatatc attattcatc tatcgtgagc agatgggatg gtcattctga
      361 taaaggagaa gctcctgctg gaaaaacctc ctatgcatgg atttggacta aatggggaga
      421 acaagtagca ttttactgtg attatgacta atcgttcatt gatttaaatc gttccccttg
      481 taagaaatta cagggggaat tttgtttaca aggagtctac tatgaagaaa ctattcatat
      541 tattatcaac tttttttctc agcttcttcc ttgcttggat tattgtctta cgtgcgccac
      601 aatatttata tgcaagctat gattccgttt ctttacttcg tgtcaaaaaa gatactcagg
      661 aaccgacgcg tgaggtattt gaacaggaat tggagaattt tgcaaactca gaacagagtt
      721 taatagctag aagaattgta gagccgagta aggatgggac gactcacttt acttatgcaa
      781 cttatggtca gggaacttta ccaaaagaat tccaagaagc tagccaagaa agtcgtgaac
      841 gtagtgatcc gctaaatagt tatctccttt tgtcaggctc cttgacgaaa gaaaagcttg
      901 ccgataaatt aggagatttg ggttataaag caagtgctga ccgaaagata ccgccctatt
      961 ttcttgcttt tcgaatatta ctaaatcccc ttattttaat tagtttagca atatttggct
     1021 tatctttctt tgctttagtg attatcactc ggattaagga aatgagagca gcaggtataa
     1081 aactcttttc tggtcagact ctcttatcca tcatggggca ttctttatct actgatatca
     1141 aatggctcct tctatcagcc ctcctttcct tcctaggtgg gggagtcgtt ctttttagtc
     1201 aaggtttgtt ttatcctatc ttgttagcca cctatggttt tgggattagt ttctatctgt
     1261 tgtttttatt ggcgatttca attttactaa tgctccttta cctaatgagt ttgagttaca
     1321 aagcattagt tcccgttatt aagggaagat taccccttaa acgcttgatg gctttaaccc
     1381 tattgtgtca gttagtagct gtttttacag taggctacgc tgttaagaca ggtttgacgt
     1441 cttaccaacg attgaaagaa cttgaaattt caaaacaagc atggcaggac agagcagact
     1501 attaccagat ttcctttggc ttaggtgata gaggaaaaga tacagaaaat cagagcaagt
     1561 ggtatgcctt tgccaaggaa gcaatcgaag aagaacaagc tctttatgta aaggataatc
     1621 tgctccattt tgccaatcca caaggaaaaa atgaacaggg agagacactg gatacctata
     1681 gtccagatgc taatacgctc tatgttagtc ccagttattt ggacaaggaa aaggtcgtgg
     1741 tagatgctga gaccaaacag aagttagccc atctccaaaa aggtgagttt atcctcttgc
     1801 tcccagaaca tttgcgctct cgagaagcag aacttaagaa agtttttgaa gaaagattga
     1861 gttattatgg aaaatctggt gaggaggcaa gtgctccttt ggattatgag atgaaagcgc
     1921 atgttagtta tctttcaatg ggagaaaagc ggtttgttta taataacggt gagaatcccg
     1981 tatctactca gtatttgact gatccgattt tagtggtatt cacgccgact tctacaggtg
     2041 atagttttat atccttatct agttggtcta tcaatgctgg aaaacaactc tttatcaaag
     2101 gatatgagag tgggctagaa ctcttgaaga aagctggaat ttatgagcaa gtatcctatc
     2161 ttaaagaagg aagaagtgtt tatctaactc gttataatga agttcaaact gaaacagcaa
     2221 ctttaatctt aggagctatt gttggaatag ccagttcctt gttactcttt tattctgtca
     2281 atcttctata tttcgagcaa ttccgccgag atatcttgat taaacgaatt tcaggtttac
     2341 gattttttga aacacatgct cagtatatgg ttagccaatt tgctagtttt gtatttggtg
     2401 ctagtctctt tattttaagc agtcgagact tggtgattgg cttgctcact ttattggtct
     2461 ttctagctag tgcagttttg acgctttacc gtcaagcgca gaaagaatct cgtgtttcta
     2521 tgacaattat gaaaggaaaa taggatgatt gaactaaaga atatatctaa aaaatttgga
     2581 agccgtcagc tattttcaga tatgaatctt cattttgaag gtgggaaaat ttatgcctta
     2641 atcggtacaa gtggctgtgg taagacaaca ctcttgaata tgattggacg attggagcca
     2701 tatgacaaag ggcaaatcat ctatgatggc acttctctta aggacatcaa gccttctgtt
     2761 ttctttagag attacttagg atacttattt caagattttg gcttaattga aagccaaacc
     2821 gtcaaagaga atctcaatct gggtttagtt ggtaaaaagt tgaaggaaaa agagaaaatc
     2881 tctttgatga aacaagctct aaaccgtgta aacctctctt atttggattt gaagcaacct
     2941 atatttgagt tatcaggagg agaagcacaa cgtgttgcac tagcgaagat aattttaaag
     3001 gatccacctt tgatcctcgc agatgaacca accgcttcct tagaccccaa aaactctgag
     3061 gaattacttt ccatcctaga atctttaaaa aatccgaatc ggaccattat tattgcgacc
     3121 cataatcctc tgatttggga gcaagtggat caggtcattc gagttaccga tttatcacat
     3181 agatgatatg gtaagattca gttagaagaa agagtcacaa acacactttg tggctttttt
     3241 atttccataa aaatggtaaa atagtaggag tagaaatgga gttcgagaca tgaaagtaat
     3301 agatcaattt aaaaataaga aagtccttgt tttaggtttg gccaagtctg gtgaatctgc
     3361 agctcgtttg ttggacaagc taggtgccat tgtgacagta aatgatggga aacctttcga
     3421 ggacaatcca gctgcccaaa gtttgctgga agaagggatc aaggtcatta caggtggcca
     3481 tcctttggaa ctcttggatg aagagtttgc ccttatggtg aaaaatccag gtatccccta
     3541 caacaatccc atgattgaaa aggctttggc caagggaatt ccagtcttga ctgaggtgga
     3601 attggcttat ttgatttcag aagcaccgat tattggtatc acaggatcga acggtaagac
     3661 aaccacaacg actatgattg gggaagtttt gactgctgct ggccaacatg gtcttttatc
     3721 agggaatatc ggctatccag ctagtcaggt tgctcaaata gcatcagata aggacacgct
     3781 tgttatggaa ctttcttctt tccaactcat gggtgttcaa gaattccatc cagagattgc
     3841 ggttattacc aacctcatgc caactcatat cgactaccat gggtcatttt cggaatatgt
     3901 agcagccaag tggaatatcc agaacaagat gacagcagct gatttccttg tcttgaactt
     3961 taatcaagac ttggcaaaag acttgacttc caagacagaa gccactgttg taccattttc
     4021 aacacttgaa aaggttgatg gagcttatct ggaagatggt caactctact tccgtggtga
     4081 agtagtcatg gcagcgaatg aaatcggtgt tccaggtagc cacaatgtgg aaaatgccct
     4141 tgcgactatt gctgtagcca agcttcgtga tgtggacaat caaaccatca aggaaactct
     4201 ttcagccttc ggtggtgtca aacaccgtct ccagtttgtg gatgacatca agggtgttaa
     4261 attctataac gacagtaaat caactaatat cttggctact caaaaagcct tgtcaggatt
     4321 tgacaacagc aaggtcgtct tgattgcagg tggtttggac cgtggcaatg agtttgacga
     4381 attggtgcca gacattactg gactcaagaa gatggtcatc ctgggtcaat ctgcagaacg
     4441 tgtcaaacgg gcagcagaca aggctggtgt cgcttatgtg gaggcgacag atattgcaga
     4501 tgcgacccgc aaggcctatg agcttgcgac tcaaggagat gtggttcttc ttagtcctgc
     4561 caatgctagc tgggatatgt atgctaactt tgaagtacgt ggcgacctct ttatcgacac
     4621 agtagcggag ttaaaagaat aatatgaaaa aaattgtctt tacaggtggg gggacggttg
     4681 gacacgttac cctcaatctt ttgttaatgc ccaagttcat cgaagatggt tgggaagtcc
     4741 actatatcgg ggacaagtgt ggtatcgaac accaagaaat ccttaagtca ggtttggatg
     4801 tcaccttcca ttctattgcg actggaaaat tgcgtcgcta tttctcttgg caaaatatgc
     4861 tggacgtctt caaagttggt tggggaattg tccaatcgct ctttatcatg ttgcgactgc
     4921 gtccacagac ccttttttca aaggggggct ttgtctcagt accgcctgtt atcgctgcgc
     4981 gtgtgtcagg agtgcctgtc tttattcacg aatctgacct gtctatgggc ttggccaata
     5041 aaatcgccta taaatttgcg actaagatgt attcaacctt tgaacaagct tcgagtttgg
     5101 ctaaggttga gcatgtggga gcggtgacca aggtttcgga caaaaacact ccagaaccag
     5161 atgaattggt ggatattcaa acccacttta atcccaaatt gccaactgta ttgtttgttg
     5221 gcggttctgc aggcgctcgt gtctttaacc aattggtgac agaccataag aaagaactaa
     5281 cagagcgcta caatattatc aatctaactg gagattctag tctgaatgag ttgaggcaaa
     5341 atctttttcg tgttgactat gtgaccgatc tctatcaacc cttgatggaa ttggctgata
     5401 ttgttgtgac acgaggtggt gccaatacga tttttgagct cttggcgata gcaaaattgc
     5461 atgtcattgt gccgcttggt cgtgaagcta gtcgtggtga ccagattgaa aatgcagctt
     5521 actttgttaa gaaaggctat gcagaagacc ttcaagaaag cgatttgacc ttggatagtt
     5581 tggaagagaa gctttctcac ttactaagtc acaaggaaga ttaccaagct aagatgaaag
     5641 cttctaagga attgaaatct ctagcagatt tttatcaatt gttgaaaaaa gatttatcat
     5701 aaggaaagta aatgtcaaaa gataagaaaa atgaggacaa agaaaccctc gaagaattga
     5761 aagagttatc agaatggcag aaacgaaacc aagaatatct aaaaaagaag gctgaagaag
     5821 aggcggctct agctgaggag aaggaaaagg aaagacaagc tcgaatggga gaagaatctg
     5881 agaagtcaga ggataaacag gaccaggaga gtgaaacaga ccaggaagat tcagaatcag
     5941 ctaaggaaga gtctgaagaa aaagtagcat cctcagaggc tgacaaagag aaagaagaga
     6001 aagaagaacc agagtctaaa gagaaggagg aacaggataa aaagcttgct aaaaaggcta
     6061 caaaggaaaa accagccaaa gcaaagattc ctggtatcca tatcttgcga gccttcacga
     6121 ttttatttcc aagtctgctt ctattgtttg tctctgccta cttgctcagt ccttatgcga
     6181 ccatgaaaga tattcgtgtt gagggaacgg tgcaaactac agctgatgat attcgacagg
     6241 cttcaggcat tcaggattcg gattatacga ttaaccttct gctagacaag gcaaaatatg
     6301 aaaagcagat taagtctaac tattgggttg aatcagctca acttgtctat caatttccaa
     6361 ctaagttcac tattaaggtc aaggaatatg atattgtggc ctactatatt tctggtgaaa
     6421 atcattatcc tattctttcc agtggtcagc ttgagactag ttctgtgagt ctgaacagtt
     6481 taccagaaac ttatttatca gttctcttta atgatagtga acaaatcaaa gtttttgtct
     6541 cagaacttgc tcaaattagc ccagaactca aggcggctat ccaaaaggtg gaattagccc
     6601 caagcaaggt gacatccgat ttaattcgat tgaccatgaa tgattcggac gaagtcttgg
     6661 ttcctctatc tgaaatgagt aagaaactgc catattacag taagattaag cctcaattgt
     6721 cagaaccgag tgtggtcgac atggaagctg gaatctacag ctacactgtg gcggataaat
     6781 taattatgga agctgaggaa aaagccaaac aagaggccaa ggaagctgag aaaaaacaag
     6841 aagaagaaca gaaaaaacaa gaggaagaga gcaatcgaaa ccaaacgacc cagcgttcat
     6901 cgcgtcgcta ggtttacctt ttctcttata gttctttagt gaccatgttt ttacgtttaa
     6961 tatttgacat ctgtttttct ttatgttaca tctgcaatgt aatcgattac aaaataattt
     7021 ttgatgaaga aggtaacaca tatgtcagat gaagtattct tatttgaaga aattgaagaa
     7081 atcgtagccc ctacagatgg tgaatttttg ggggaagttt tacttggaac tggggtagtt
     7141 ctcttaattg gagtagcctg ttgttaaaaa gatagggagt gataatcatg caagataact
     7201 ttttatttga ggaaattgaa gaaatttcag taccagttag tgatttttca gctggacttg
     7261 caacaggtat cggatttggt ttagcaatcc ttgctcttgc tggttgttga agtttgttca
     7321 tttactaaca tcaagctttt tcaatttcat tttagacagt catttaaatt ttccgtatta
     7381 gtcttgcagc aagagattaa tagaattagt cattatttta ttgattgcgg actgagggac
     7441 tagagtatgt tttacttaac ccctctttta tttattaaag gttaggtttg ttatgagaat
     7501 tgttgataag attaagatat tacctactcc ttatgaggga cactatcatt tatatatacc
     7561 atccagtaag aaacatgtat tagttgggaa acaggaaaaa aatggttaga gcaactaata
     7621 ggtcaagaat ttaccatatc ggacttatta gtgttagtag ggaagaaat
//
LOCUS       DAVHEW010000097         7596 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVHEW010000097 DAVHEW010000000
VERSION     DAVHEW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.097"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(124..1323)
                     /locus_tag="VVL53_001817"
     CDS             complement(124..1323)
                     /locus_tag="VVL53_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183944.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(1320..2213)
                     /locus_tag="VVL53_001818"
     CDS             complement(1320..2213)
                     /locus_tag="VVL53_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183945.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFSILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            2330..2410
                     /locus_tag="VVL53_001819"
                     /pseudo
     CDS             2330..2410
                     /locus_tag="VVL53_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2920..3114
                     /locus_tag="VVL53_001820"
                     /pseudo
     CDS             2920..3114
                     /locus_tag="VVL53_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3529..4146
                     /locus_tag="VVL53_001821"
     CDS             3529..4146
                     /locus_tag="VVL53_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU4183946.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(4466..5353)
                     /locus_tag="VVL53_001822"
     CDS             complement(4466..5353)
                     /locus_tag="VVL53_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4183947.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(5502..7385)
                     /locus_tag="VVL53_001823"
     CDS             complement(5502..7385)
                     /locus_tag="VVL53_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU4183948.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKRAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IVVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
BASE COUNT         2264 a         1665 c         1406 g         2261 t
ORIGIN      
        1 tattccagat tcagttcact atacaattga gttttcaagc aacctgttta cataatgtgt
       61 acataattag gttcgtgatt ccaccctttt cacctttaaa aacctcgctt tcgcaaggct
      121 cttctattta taagataagg cacgtttaaa ggttttccaa atccctaaat catccgtttg
      181 aagaacgaga ctagcataca tgcgtccgat aaatcctgtt gctaccaccg caaaaatcac
      241 tgtaatagca agtgaaatcc atgcttctgc tccccccgca tagtcattaa tcgttcgaaa
      301 cggcataaag aaggtcgaaa taaagggaat ataagaacca atcttcaaga ggagattgtc
      361 accagctgca cctagagctg tcactccaaa aaaaccaccc ataatcaaaa tcatcaaagg
      421 cgacaaggct ttccctgagt cctcaggacg agaaaccata gatcctagga aggctgccaa
      481 gactacgtac atgaaaagac tgatcaaaat aaagagcaag gtattcagtg agatagcatc
      541 tcccaagtga tccaaaatac cagactgagc caagaatggc aaatctttaa agagcaaaac
      601 ggcagccaga ccacctacaa catagatccc aatatgcgtt aaaatcacta gaaacagagc
      661 catcatccgc gcatagaaat agtgacttgc ccttatgcta gaaaaaacga cttccataat
      721 tttggtgcct ttttcactgg caacttcctg agctgttaca cccgcatagg taatcagaat
      781 catataaaga aagaatccta aggcacctgc tgcaattgtt tgaataaact ttttattttc
      841 cttggcttca tcaatctttt ctgtgaattg aattgtctgc gctaagcgtt tttcctgctc
      901 ttgagacaag gaagcagttg aacgattaag ctgattttgc agttcattga gtgtacctgt
      961 aacctcaaat ttaattccat tttcaagcga tgtttcgcca tgataaactg cctttagaac
     1021 actatcttct tgatcaatgg tcaaataacc ttttaatttt tcttctttaa ttgcttcttt
     1081 ggcacttgct tcgtctttat agtcgaagtt aacaccattt acattcttca gtccttctgc
     1141 tacagatggc actgttgtca ctactgccac tttattattt ttagccatag aagaaccttg
     1201 gagatgccca attcctacag agattcctaa aaagaggaac ggcgaaatca ccataaagaa
     1261 gaaactccat gactcgacat gtcgaagata ggtttccttg attacaaccc acatatttct
     1321 catacttcca ctcctgattc tagtttaaag atttcatcga tagttggcgc ttgttggtca
     1381 aatgttgcga tatattgacc ttgagtcaag attgagaaga gttcccttcc agcgctctca
     1441 tcctccaaaa tcaatttcca actgccttgt ttggtcaagc tcacctgttt gacatgagga
     1501 agattttcca attcttcctt gcttcgttca cttgaaacaa agagacgcgt tttcccgtat
     1561 tgattgcgga catcctgaac tggtccgtgc aagaccacac ggccatctcg gatcatcaga
     1621 atatcgtcac aaagttcctc aacattggtc atgacatggt cagaaaagat aatggttgct
     1681 ccgcgctctt tttcctgaaa aatgacttgt ttgagcaatt ctgtattaac tgggtccaat
     1741 ccactaaaag gctcatccaa gataatcagg tctggttcat gaatcagagt aataatgagc
     1801 tgaatcttct gctgatttcc ttttgacaga ctcttgattt tatctgtcag ctttcctttc
     1861 acttccaacc tcttcatcca ttgagggagt ttttctttga cttctttggc atccatgcct
     1921 tttagagtcg ccaagtagcg aacttgttca agaactgtca atttaggcat gagactgcgt
     1981 tcttcaggca gataaccaat ccgagcatag gtctcctgac gaatatcctg accatccaga
     2041 ccgatttctc cctgatattc taggaatttc aaaatactat ggaaaatcgt tgtttttcca
     2101 gcaccatttt ttccgactag tcccaaaata cgacctggtc gcgcttgaaa gtcaatacca
     2161 aacaaaactt gcttggatcc aaaacttttc tctagacttc ttacttctag catctttcac
     2221 ctccgaaatt tcttgcactc attatactcc tttttgatag cctttacaat gttttttgtc
     2281 catttttaga agactattgc tgtgtaaaat atggcctgga gcacttttat actcaatgaa
     2341 aatcaaagag caaactagga agctagccgt agactgctca aagtacagct ttgaggttgc
     2401 agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgtggata gaactgacga
     2461 agtcagtaac tatatctacg gcaaggcgaa gctgacgtgg tttgaagaga ttttcgaaga
     2521 gtattagtga taaatccatt atacagcagc aaacttaatt tataccttcc gctcctcaac
     2581 tgtctatttt taatcctgaa ttgttatttg agtaactcct ttttcctcgt aaagttttct
     2641 tcctctaaaa cttctggaaa aaggctaata gtttcagaca acatttttat aagaaacaag
     2701 ttcatctgtc atttcaagaa ggagtaatcc tttatctact aatggacgga acagaattca
     2761 accgcttgtc cgatatgttt tctaaggatt atatagtaaa atgaaataag aacaggacaa
     2821 attgatcagg acagtcaaat tgatttctaa caatgtttta gaagtagatg tatactattc
     2881 tagtttcaat ctgctatatc tattatgcac acccctatag gatctaatga aaatcacaac
     2941 aggctcattc atagatggtt acctaagcct aagggaacta agaaaacgac taccaaggaa
     3001 gtcgcattca tcgaaaagta gattaacaac tatcctaaaa aatgcttgaa ctacaagtcc
     3061 cccagagaag acttctggat gactaacttg aacttgaaat ttagcaataa ttaattcact
     3121 atctaactat atttagtaat tatttcagaa ctgattaata ttaaaattaa ctaacaattc
     3181 aaaggattca tactagccat aaattacgtc catcagagag agactcttac tacttttaga
     3241 ttttagtctt tctagcttca gaatacatct aaactttagg gaaaatgact attcgaaagc
     3301 gcgaatgcct caaaattatc tcagataagc tattcgaaac ttagaatgct tttaaattta
     3361 tggaattgcg attattcgaa acctagaatg catataacct ttagttgaca gacctattct
     3421 aagtctcgaa gggctattta ctttctattc cttatcaaaa aagactcatt ccccctttct
     3481 cctccaaaat atggtatagt agaaatatac tatctatgag gagtttacat gtcacaggat
     3541 aaacaaatga aagctgtttc tccccttctg cagcgagtta tcaatatctc atcgattgtc
     3601 ggtggggttg ggagtttgat tttctgtatt tgggcttatc aggctgggat tttacaatcc
     3661 aaggaaaccc tctctgcctt tatccagcag gcaggcatct ggggtccacc tctctttatc
     3721 tttttacaga ttttacagac tgtcgtccct atcattccag gggccttgac ctcggtggct
     3781 ggggtcttta tctacgggca catcatcggg actatctaca actatatcgg catcgtgatt
     3841 ggctgtgcca ttatctttta tctagtgcgc ctatacggag ctgcctttgt ccagtctgtc
     3901 gtcagcaagc gcacctacga caagtacatc ggctggctag ataagggcaa tcgttttgac
     3961 cgcttcttta tttttatgat gatttggccc attagcccag ctgactttct ctgtatgctg
     4021 gctgccctga ccaagatgag cttcaagcgc tacatgacca tcatcattct gaccaaaccc
     4081 tttaccctcg tggtttatac ctacggtctg acctatatta ttgacttttt ctggcaaatg
     4141 ctttgacacg taaaaaatcc gtttggtttc ccaagtggat ttttaaagcg tagattaact
     4201 atagcttgat actaaatata ctttggtatg gaaatcatgc atatttttcg atagtgaggc
     4261 gaggacttac ctagcctttc cgccgtgata gaaacacctg aaatctaatg gtttcaggta
     4321 ttcggaaact ttgagcctag tgtctcaaag tttaggtatg gaattttgaa gaaagtcgct
     4381 accgtccgta atcacttaag gaaaggctca aaaatattgt tttcaaccac aaaatccgtt
     4441 tggtttccca agcggatttt gtgctttatt ttgaaacttc ttttgcaaga acaaagttcc
     4501 caagtgtggc agaaccattt cctgcgactg ctggcgtcac gatatagtca cgcacatctg
     4561 gtactggtag gtaaccatta agaagagatg taaatttctc acggacacgg tccagcatat
     4621 gttgttgagc catgacccct ccaccaaaga caatcacgtc tgggcggaaa gtcactgtcg
     4681 cattaaccgc agcttgagcg atatagtagg cttgaacatc ccaaacaggg ttgttgagtt
     4741 caatagtttc cccacgtaca cctgtacgag cttccaaact tggaccagct gcataacctt
     4801 ctagacatcc cttatggaaa ggacaaacac ccttaaactc tttttcaata tccattgggt
     4861 gtctagcaac ataataatga cccatttcag ggtgacccac accaccgata aactcaccac
     4921 gttggatgac gcctgcaccg atacctgtac cgattgtgta gtaaaccaag ttttcgatac
     4981 gaccaccagc attgttacgg gcaaccattt caccgtaagc agagctgttt acgtctgttg
     5041 tgaagtacat tggcacgttt agggcgcgac gaagggcacc aagcaagtct acatttgccc
     5101 agtttggttt tggagtcgtc gtgataaagc cataagtttt tgagtttttg tcaatatcaa
     5161 tcggcccaaa tgaaccaact gcaagaccag caaggttatc gaattttgag aagaactcaa
     5221 tggttttatc gattgtttcg attggagttg ttgttggaaa ttgtgttttt tctacaacgt
     5281 taaagttttc atcaccgaca gcacagacaa actttgtacc gcccgcttcc aagcttccat
     5341 ataattttgt catgataaac ctcttgtttt tattttcttt attatagcat acttcgaaag
     5401 tctaaatgtc tctatttttt agattttcct ctgtaaatct tactatctaa taaaaacgaa
     5461 caaacatgtc atttgttcgt tttcacatta gagaggattg attagatttt cacttcgatc
     5521 acagcatccc ccttagcaac tgaacctgtt gcgactggag ctactgaagc gtagtcacct
     5581 gtatttgtaa cgataaccat tgttgtatca tcaagtccag ctgcagcgat tttgtttgag
     5641 tcaaatgttc caagaacatc gccagctttc accttattac cttgagcaac ttttgtttca
     5701 aaaccgtcac cgttcataga tacagtatca ataccaacat gaatcaaaac ttcagcacca
     5761 tttcttgttt tcaaaccaaa agcgtgccct gttggaaagg caattgaaac ttcagcatca
     5821 gctggtgcat agaccacgcc ttggcttggt ttcacaacga taccttgtcc catagctcca
     5881 cttgagaaga ctgggtcatt gacatcagca agagcgacaa catcaccgac gataggagtt
     5941 acaagtgttt cattttgaag agctgctggc gcaacttctt ctttttcttc agccacttca
     6001 gctcgttttg cagctgcagt tgcgtctact tcatcttcgt aaccaaacat gtaagtaaga
     6061 gcaaaaccaa gggcaaatga tacagctacc ataagaaggt attgtggaag ttgtccgtta
     6121 ccaacataaa gcattgtacc agggatgatg gtgataccat taccagtacc agcaagtcca
     6181 aggatagaag ccaatccacc accgattgca ccagcaatca atgaaaggaa gaatggttta
     6241 cggaagcgca agttcacccc gaagatagca ggctctgtaa tacctaggaa ggcagaaaga
     6301 gcagccggga aagcaagtgt tttcagtttt ggattttttg ttttaacacc aaccgcaaca
     6361 gtagcagcac cttgagctgt catagcagct gtgatgatag cgttgaatgg gttagcatgg
     6421 tcagcagcaa gtaattgcac ttcaagcaag ttgaagatgt ggtgcacacc tgacacgacg
     6481 atcaattggt gaaccccacc aatcaagaaa ccaccaagac caaatggcat gctaagaatc
     6541 gcttttgtag caataaggat gtagttttca acaacgtgga aaactggtcc aatgacaaag
     6601 agtccaagga tagacatgac caaaagtgtc acgaatggtg ttaccaagag gtcaatgaca
     6661 tctggaacaa ccttgcggac agctttttca aatttagctc cgacaacccc gatgatgaag
     6721 gctggaagaa cggaaccttg caaaccaaca acagggatga aaccaaagaa gttcatcgct
     6781 gttacttcac caccttgagc aactgcccaa gcgtttggaa gtgagccaga gacaagcatc
     6841 ataccaagaa cgataccaac ggcaggattt ccaccaaata cacggaaggt tgaccacaca
     6901 accaaacctg gcaagatgat gaaggctgta tctgtcaaga tttgtgtgta agttgcaaag
     6961 tcacctggaa gtggcatttc aagagcgttg aaaagaccac gcacacccat gaagagacct
     7021 gtcgctacga taactgggat gattggaacg aaaacatcac caaaagtacg gatagcacgt
     7081 tggaaccagt tcccttgttt agcaacttct gctttcatgt catccttaga tgatgttggt
     7141 aatccaagta caacaacttc atcgtacatt ttgttaactg tacctgtacc aaagataatt
     7201 tggtattgcc ctgagttaaa gaaagcacct tgaacttttt ccaagttctc aatcacttct
     7261 ttattgattt tctcttcatc tttgaccatg acacgtagac gagtcgcaca gtgggcaaca
     7321 ctattgacat tttcacgtcc gcccaaggca tcgatgactt tttttgcaat ttcctgattg
     7381 ttcatttgca aaaatctcct tatataacat tttgttcttg tttgaaagcg attttattcg
     7441 cccttacgac tattatttta tcatgtttta aaaatatgtc aagcgttttg cagaaaaaaa
     7501 ttctatccac ttagtgggca agctttagat tgattgtttt tgactgtttc atcaggaatt
     7561 ccttgaaaaa taagtttgaa accttggtta aaaagt
//
LOCUS       DAVHEW010000098         7299 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVHEW010000098 DAVHEW010000000
VERSION     DAVHEW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.098"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            149..1216
                     /locus_tag="VVL53_001824"
     CDS             149..1216
                     /locus_tag="VVL53_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183949.1"
                     /translation="MTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVLLLF
                     LFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIRVGI
                     NLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFPLLR
                     RTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFPMSV
                     YEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIVVLL
                     ALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGLYLY
                     LKHEDIKTYLAYFKASKKTSN"
     gene            1281..2369
                     /gene="wecB"
                     /locus_tag="VVL53_001825"
     CDS             1281..2369
                     /gene="wecB"
                     /locus_tag="VVL53_001825"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU4183950.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            2532..3401
                     /gene="rfbA"
                     /locus_tag="VVL53_001826"
     CDS             2532..3401
                     /gene="rfbA"
                     /locus_tag="VVL53_001826"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU4183951.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            3402..3995
                     /locus_tag="VVL53_001827"
     CDS             3402..3995
                     /locus_tag="VVL53_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU4183952.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            4007..5056
                     /gene="rfbB"
                     /locus_tag="VVL53_001828"
     CDS             4007..5056
                     /gene="rfbB"
                     /locus_tag="VVL53_001828"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU4183953.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFTDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDLRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQVGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQDWWKAEKE
                     AVEANYAKTQEIITV"
     gene            5122..5973
                     /gene="rfbD"
                     /locus_tag="VVL53_001829"
     CDS             5122..5973
                     /gene="rfbD"
                     /locus_tag="VVL53_001829"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU4183954.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            6036..6370
                     /locus_tag="VVL53_001830"
                     /pseudo
     CDS             6036..6370
                     /locus_tag="VVL53_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            6637..7222
                     /locus_tag="VVL53_001831"
                     /pseudo
     CDS             6637..7222
                     /locus_tag="VVL53_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         2299 a         1259 c         1566 g         2175 t
ORIGIN      
        1 tggtggctta gaatataata atttagatag aaaacagttt tattctcaga tgattctatt
       61 ttctagcttc ttaatgatta tctccttgct attgtttggt ttaaatgtat tagctgtaac
      121 agattcgaat gcacgatata tttataatat gactattata agtatgataa tcacaaattt
      181 aagaacgctc tacgtgtatg ttttgcagat gacaaaccgc ttgaaggata gttccatcat
      241 tctactcagt gatcgcgttt tatatgtgct ccttttattc ctgtttattg tatttggttg
      301 gcatgaatac aaggttatga ttttggcaga tattctaggt cgatcatttt ctctcatact
      361 ttccttctgg atttgtaaag atattgtgtt tcagcctttg tcaaaattca tctttaatat
      421 aaaagagtct tttgataata tccgagttgg tatcaattta atgttatcca acatcgcaag
      481 tagtttgatt atcggtattg ttcgtctagg aatccaatgg aactggaata tcgaaacatt
      541 cgggaaagta tctctgacct tgagtgtttc caatctattg atgactttca ttaatgccat
      601 tggactagtc gtctttccgc tattaagacg aacaaaagca gaaaacttac ctaagattta
      661 ttctaattta agaaacgttt tgatgcttat catgttcgcg attttgctca tttactatcc
      721 tttaaaaatt gtattagacc tctggttgcc agcctatcaa gatgccttga ttttcatgac
      781 ccttattttc cctatgtcag tctatgaagg gaaaatggca ttggtcatta atacttactt
      841 aaaggcatta agaatggaaa gagatatcct tagagtcaat gccttagtaa tgttgatcag
      901 tatgggagtg accctggtta caacatacct gttaaatagt ttggagctga ctgttgtatc
      961 gatagttgtt ttgctagctt tgagaagtat catagccgaa ttaattctgt ctaaaaaact
     1021 ggatgtttcg gttaagaagg atattgtatt agaatttctt ttgacgcttg tctttatttc
     1081 ttcaagttgg tatttgccga ttgggctggc agtaattgtc tacacaatag cctacggttt
     1141 atatctctat ttgaaacacg aagatatcaa aacctattta gcttacttta aagctagtaa
     1201 aaaaacatca aattaaaaaa ttatataatg attaagtggt agattctatt tctaccgttt
     1261 tagatattcg ggaggtaatg atgaaaaaaa ttatgttagt attcggtaca cgtccggaag
     1321 ccatcaaaat gtgtccatta gtcaatgagt tgaaaaaaca cgaagatatg gaaacaattg
     1381 tgtgtgttac tggacaacac aaagagatgg ttagtcctgt tttagattta tttggtgttg
     1441 taccagatta tgatttagaa attatgaagg ctaaccaaac cttgttctct atcacaacta
     1501 gtatcttgga aaagataaaa ccagttttag agaaggaaca accagatatt gtcctagttc
     1561 acggtgacac tacgacaact tatgcagcag ccttggcagc attctatttg ggaattaaag
     1621 taggacatgt tgaagctggt ttgcgaacgt acaatttaca aagtccattt cctgaagaat
     1681 ttaacaggca atcgacatca atcattgcaa cttaccattt tgctccaact gagttggcta
     1741 aagaaaatct cttaaaagaa ggtagagaga atgtttatgt gactggaaat actgtcattg
     1801 atgctcttac aactactgtt caagaggatt atacacacac tcatttagat ttaaacgcta
     1861 acaatcgtct catcttattg actgctcata gacgcgaaaa tctcggcgaa ccgatgagac
     1921 atatgtttag agcagttaaa cgagtattga atgaatacga agatgttaaa gtcatttatc
     1981 caattcataa gaatcctttg gtacgtgaaa cagctgcaga gatctttgga gatacagaac
     2041 ggattcaaat tattgaacct ttggatgttc ttgatttcca caacttcatg aaccaaagtt
     2101 atatgatttt aacagattct ggcggagttc aggaagaagc accttcttta ggaaaacctg
     2161 tattggttat gcgcgatact actgaaagac ctgaaggagt agcggcagga accttgaaat
     2221 tagtaggaac tgacgaagaa acaatttatc aaaacttcaa actacttcta gatgattctg
     2281 gagaatacaa aaaaatgagt caggctagca atccatacgg taatggtgat gctagtcaac
     2341 agattgttca gattttgcgt gggatttaag aagattctct aaaagagtct aagaggaatc
     2401 cactccacaa acataaaact cttatgctat catttatgat tgagatagac tcataactta
     2461 atatttttat cctaagaaaa ttcgttcata ttcaatatga atgaaaaaca acgaagaaag
     2521 gttatttcat tatgaaaggt attattcttg caggcggctc aggtacccgc ctgtacccac
     2581 ttactcgggc tgcgtcaaaa cagctgatgc cggtttatga taaacctatg atttattatc
     2641 cgttgtcgac attaatgttg gctggaatta aagatatttt gattatctca actcctcaag
     2701 atttgccccg ttttaaggac ttgctcttgg atggttccga atttgggatc aagctttcct
     2761 atgcggaaca acctagtccc gatggacttg ctcaggcttt tcttatcggt gaagaattta
     2821 tcggtgacga tagcgttgcc ttgattttag gcgacaatat ctaccatggt cctggtctga
     2881 gcacaatgct tcaaaaagca gccaagaaag agaaaggtgc gactgttttt ggctaccaag
     2941 tgaaggatcc agagcgtttt ggtgtggttg agtttgatac agacatgaat gctatttcta
     3001 tagaagaaaa accggagtat cctcgctcca actatgcagt gacaggactg tatttctatg
     3061 ataatgatgt tgtggagatt gctaaacaga tcaaacctag tgctcgtggc gagttagaaa
     3121 ttacagacgt taacaaggct tacctaaatc gtggtgacct ttctgttgag ctgatggggc
     3181 gtggttttgc ctggttggat acgggaaccc atgaaagctt gctagaagct tctcagtata
     3241 ttgaaacggt tcagcgtatg cagaacgttc aagtggcaaa tctagaagaa atttcctatc
     3301 gtatgggcta cattagtcgt gaagacgtgc tggaattggc tcagcctctt aagaaaaatg
     3361 aatacggaca atatttgctc cgtttgattg gagaagcata gatgtcagat aatttttttg
     3421 gaaagacact tgtggtacgc aagattgatg ctataccagg actgctagag tttgatattc
     3481 ccgttcatgg agacaatcgt ggttggttta aggaaaattt ccagaaggaa aagatgctac
     3541 cgcttggttt tcctgaaagc ttctttgctg cagggaaact gcaaaataac gtcagctttt
     3601 ctcgcaaaaa tgttcttcga ggactccatg ctgaaccttg ggacaagtat atctctgttg
     3661 cagacgatgg gaaggtttta ggatcttggg tagatttgcg tgaaggcgag acctttggaa
     3721 atgtttacca gacagagatt gatgctagca agggaatttt tgtccctcgt ggcgtagcca
     3781 acggttttca agtcctctca gatacggttt cctatagcta tctggtcaat gactactggg
     3841 ctcttgaact caaacccaaa tatgcctttg ttaactatgc tgacccagca ttgggaattg
     3901 agtgggaaaa cctagcggaa gctgaggttt cagaggcaga caaacaccat cctctattaa
     3961 gggatgtcaa accacttacg aaagacgagt tgtaaaagga aacattatga ctgaatacaa
     4021 aaaaattatc gtgacaggtg gagctggttt tatcggttct aactttgttc actatgttta
     4081 caataacttt acagatgtcc atgtgacagt gctggacaag ctgacttatg caggtaatcg
     4141 tgccaatatt gaagaaattt taggcgaccg tgttgagtta gtcgttggag atattgctga
     4201 tgcagccttg gtagataagt tagcagctga agcggatgcc attgtacact atgcggcaga
     4261 aagccacaat gacaattcgc ttaatgaccc ctcgccattt atccatacca acttcatcgg
     4321 aacttataca ctcttggaag cggctcgtaa atacgacctt cgtttccacc atgtgtcgac
     4381 tgacgaagtc tatggggatc tgccgctgcg tgaagattta ccaggtcatg gggaaggacc
     4441 aggtgagaaa tttacggctg aaaccaagta caatccaagc tcgccttact catcgactaa
     4501 ggcggcttca gacttgattg tcaaagcttg ggtgcgctca tttggtgtta aagcaacgat
     4561 ttctaactgt tcaaacaact atggtcctta ccagcacatc gagaagttca ttccgcgcca
     4621 gattactaat atcttgagcg gtatcaagcc aaaactctac ggagaaggta agaatgtgcg
     4681 tgactggatt catactaatg accattcatc aggtgtttgg acgattctga ccaagggtca
     4741 agtcggtgaa acctacctga ttggtgcgga tggtgagaag aacaataagg aagtgttgga
     4801 actcatcctc aaagaaatgg gacagccagc tgatgcttat gaccacgtaa cagatcgagc
     4861 tggtcacgac ctccgctatg ccatcgatgc aagcaagctc cgtgatgaac taggatggaa
     4921 gccagagttt accaactttg aagcaggtct caaagcgact atcaagtggt acacagataa
     4981 ccaagactgg tggaaagcag aaaaagaagc tgttgaagcc aattatgcta agactcagga
     5041 gattattaca gtataaaaag caggaaatcg ctgcttttta ttgctatatt gggaagagtt
     5101 acatattaga aaggtctaga gatgatttta attacagggg caaatggcca attaggaacg
     5161 gaacttcgct atttattgga tgaacgtaat gaagaatacg tggcagtaga tgtggctgag
     5221 atggacatta ccaatgaaga aatggttgag aaagtttttg aagaggtgaa accaacttta
     5281 gtctaccact gtgcagccta tactgctgtt gatgcagcag aggatgaagg aaaagagttg
     5341 gacttcgcca tcaatgtgac ggggacagaa aatgtcgcca aagcatctga aaagcatggt
     5401 gcaactctag tttatatttc tacggactat gtctttgacg gtaagaaacc agttggacaa
     5461 gagtgggaag ttgatgaccg accagatcca cagacagaat atggacgcac taagcgtatg
     5521 ggggaagagt tagttgagaa gcatgtttct aatttctata ttatccgtac tgcctgggta
     5581 tttggaaatt atggcaaaaa cttcgttttt accatgcaaa atcttgcgaa aactcataag
     5641 actttaacag ttgtaaatga ccagtacggt cgtccgactt ggactcgtac cttggctgag
     5701 ttcatgacct acctagctga aaatcgtaag gaatttggtt attatcattt gtcaaatgat
     5761 gcgacagaag acacaacatg gtatgatttt gcagttgaaa ttttgaaaga tacagatgtc
     5821 gaagtcaagc cagtagattc cagtcaattt ccagccaaag ctaaacgtcc gctaaactca
     5881 acgatgagcc tggccaaagc caaagctact ggatttgtta ttccaacttg gcaagatgca
     5941 ttgcaagaat tttacaaaca agaagtgaga taagtagtag aatgattttc tagtctaata
     6001 aaagaggcag ataatgaact caaaaggagc ttaaggtgta cgattatctt gttgttggtg
     6061 ctggtctctt tggtgcagtc tttgcccatg aagtagcctt aaaaggaaaa aagtaaaagt
     6121 cattgaaaaa cggaatcata tcgtgggtaa tatctatact cgtgaagagg aaggaattca
     6181 agttcatcaa tatggtgctc atatcttcca tacttctgat aaggagagct gggattatgt
     6241 aaatcagttt gcagggttta accgttacac caattctcct gttgcaaact ataagggtga
     6301 gatttataac cttcctttta atatgaatac tttcaataaa ctctggggag ttgtaacgcc
     6361 agcagaagca caagctaaga ttgaggaaca acgtgctatt ttaaatggta aaactcctga
     6421 aaatttggaa gaacaggcga tttctcttgt aggtacagac atctacgaaa aattaatcaa
     6481 agactataca gagaaacagt ggggcaaacc aactactgaa cttccatcct ttattattcg
     6541 ccgtttacca gtacacctga cctatgataa caactatttt aacgatacct atcaagggat
     6601 tccaattggt ggatacactc aaatagttga aaaatgttgg attatgaaaa tattgatgta
     6661 gaaacaaatg ttgatttctt tgtgaacaaa gagcaatatc tgaaagattt tcctaagatt
     6721 gtctttactg gtatgattga tgaattcttt gactataagt tgggcgaact agagtaccgt
     6781 agtcttcgtt ttgaaaatga gaccttggat atggaaaatt accaaggaaa tgcagttgtg
     6841 aactatacgg atgcagaaac cccatatact cgcattattg aacacaaaca ttttgagttt
     6901 gggagtcaag caaagactat cattactaat agaacattct aaaacatggg aaaaaggtga
     6961 tgagccttat tatccagtta ataatgatcg taataatcat ttgtataaat catataaaaa
     7021 acttgctgat gagcaaggga atgttatctt tggtggccgc ttaggacact atcgttatta
     7081 cgatatgcac caagtaattg gagcagcttt gcagtgcgtg agaaatgagt tagattcata
     7141 ctcaatgaaa atcaaagagc aaactaggaa gctagccaca ggttgctcaa aatactgttt
     7201 tgaggttgca gatggaagct gacgcggttt gaagagattt tcgaagagta taaacaagta
     7261 aaactgacta ccagttatta tttagaaata gtattaaaa
//
LOCUS       DAVHEW010000099         6978 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVHEW010000099 DAVHEW010000000
VERSION     DAVHEW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.099"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(72..832)
                     /locus_tag="VVL53_001832"
                     /pseudo
     CDS             complement(72..832)
                     /locus_tag="VVL53_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(1105..1947)
                     /locus_tag="VVL53_001833"
     CDS             complement(1105..1947)
                     /locus_tag="VVL53_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4183955.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(2061..3452)
                     /locus_tag="VVL53_001834"
     CDS             complement(2061..3452)
                     /locus_tag="VVL53_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4183956.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(3687..4064)
                     /locus_tag="VVL53_001835"
                     /pseudo
     CDS             complement(3687..4064)
                     /locus_tag="VVL53_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            4279..5256
                     /locus_tag="VVL53_001836"
     CDS             4279..5256
                     /locus_tag="VVL53_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4183957.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            5253..6335
                     /locus_tag="VVL53_001837"
     CDS             5253..6335
                     /locus_tag="VVL53_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4183958.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(6399..6978)
                     /locus_tag="VVL53_001838"
                     /pseudo
     CDS             complement(6399..6978)
                     /locus_tag="VVL53_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2112 a         1535 c         1176 g         2155 t
ORIGIN      
        1 actgtaagga ggtctctgcc actgatgcag tgaaatgtta atttcttaga cattgtttac
       61 tcctttagtt tttatatttc tttttcaatt aatttctgct gaacaaacca atctaacaag
      121 ggaaaagttt tttctgtatt tgcccataaa tgataagaat ctaagaccgc ttgtacacta
      181 cctaaatttt ctaccaactt atctactgtc aaaaagctgc gccagtattc ataaaaaata
      241 cttgcatagt tcttatcata agtcgaagaa ccaaaatcat tcaatgaatg ccaactatac
      301 ttcttctgaa aaagttctac gagagattga ttacaaattt tttccgcttg aaattcctct
      361 tctgtcaaga aatacttgcg actaatatat tcaaccatcc cctcttcgaa ccagatataa
      421 gagtcataac catcaaaatc atctaaaaaa tgtccgacca atgagctaac tcatgtccta
      481 caatctgtaa gaggaaattt tcagataaag aatgatagtg actttctatt gcttgagttt
      541 gctcagaaca ctcgtagtcc atcaactgat acaaatacaa ttctttccaa acagctaaat
      601 caggcgtcat aaccactcgt ctattatttg tataggttgg aacagcactt tccctaataa
      661 tctgtgtagc agcattaaaa tctgaccaaa taacagcttg cggtaaatca cagactgcaa
      721 gttcattctt taaaaaagat tgataatctt tcaacttttc agtatttctt actacaaaat
      781 cacgaaatgt agctagttga ctatcgtctt ttacaagata aaggttgtcc acggctctcc
      841 tttctttcac acaatagact gaataagcaa aagaaccaca tctgacaatc ctaggttgat
      901 ttaacctagg ggcgtcagaa acgcggttcc accctaattt attacttcat tgtttttgaa
      961 aatactacag aaagcgccag ccctttattt tgccctactt ggctcccact atcccaagct
     1021 cgcttgaaga acgccataag actttctttc ctgctgacta ttatatcaaa aattagaata
     1081 atgtacaatt ctttttatga ttttctagaa atccacatca tcaactcgtg gagcacccga
     1141 ttgaacagca atcgttttta aggtttctct ctcctcatga ctcagttcaa ttccaaagca
     1201 atcaagatta gcttgaatac gagaggttgt gacagatttt ggaagtggta aaaatccttc
     1261 tgccaagctc caggccaagg ctatctgagc aaccgatttt ccgtgatttg ctgctatttc
     1321 ttggacttgc ttgctatcaa acagttctcc ttgtccaaaa ggcccccaag cttccaataa
     1381 aattcccttt tcacgacagt aagctacgac ttgatcttga tacacacctg gcgccaagcg
     1441 aacttgattg accgcaggaa cgatagttgc agtttcaagc aaggcatcca aatgatgggg
     1501 aagaaaattg ctaacgccga tagcacggat tttcccttct tgatagaggt cttccatcgc
     1561 tctccaaact tccgcattgc gagttttcca tgcgtcattt tctctgagcg gttttgggtt
     1621 cggccaatga atcaaataca aatccaaata atccaagccc agtttttcta tagatttttc
     1681 caaagcttga cgagtttgct cataggtttg ctgactattc caaagcttgg tagttacgaa
     1741 catttcttca cgtggaactc cgctatcttt gattgcttga ccaacacttt cttcattctg
     1801 ataaatcgcc gccgtatcaa tatgacgata accagccttc aaggcttcta acactgcacg
     1861 ataggcttct tctccatcct tagccttaaa agttccaaat cccaatactg gaatttctac
     1921 tccattattt aattgatagg tattcatatg cttctccttc tattttattc atcaattata
     1981 gcataaaaca ttttttaagt agtactatat tctgtaaaaa ataagtctgt caaatatcta
     2041 acagacctat tttttcttgc ttattcatca cgactatagt acagctcatg agcttttagg
     2101 cgagtattga gcatctctgg tatggtcaca aaggtataac cttgattttt caaatactca
     2161 atgacccttg gcaaggcatt gactgtcgga ctgtgaatat catgcatcaa aacgatagag
     2221 ccattagcta cttgatgctg aatttctgtc aaaatagatg cttcattttt actcttccag
     2281 tccagactat ccacatccca catgataaag ctcaaatcca agctattgcg aatatcatct
     2341 gtaatagcac cataaggtgg acgcatgagt ttagaactag aacccagcac tttagttagc
     2401 acatcctcag tatcagtaat ctgcttttta gcttcatcaa gagagagttg cgagagaatc
     2461 ggatggctcc agctatggtt tccaacaaca tgaccttcag attttatcct cttcaccaag
     2521 tcctcattcc cagaaacatt tttcccaagc acaaagaaag tcgctttaat atcatattta
     2581 gctagggtct ctaatacctg cggggtcgtt gctggatttg gaccatcatc aaaggttaga
     2641 gcgacaactt tttgatgttt cttatcaaag taagattggt acaaggccgc atctttttcg
     2701 agtaagtacg aagattggat aacatcaaag aaagcagata ctggcaaggc tatctcttct
     2761 aaattttcaa ccacaggact tggataaagg ataatctgac tatccttgta atcaaaattc
     2821 catgcagaca agtcttggtc agagaagttt tttacaatct gctcactctg gtcttgctct
     2881 atttttttat cctctatgaa ggaggtcaac tcttttatca gctgttcctt agccttacta
     2941 gcatctgaaa acagttggtc aagtgtaaaa ggttgcccat cttctgttaa gtgaaccttt
     3001 cctagactgg tcttttcagt ctcttcaatc ttaaaagcaa ctaaatcata gatttgtttg
     3061 gtcacattac gattaacgac tccctttaaa cctgactctt cttgctctgt atagtagaaa
     3121 acaagattgt ccttactttc tagcttgtcc ttaacatctt gatttatcag ctccctaaca
     3181 gaggaaatca ctttctcccc ttggagagga taataggcaa tcacttcggc ttgcccctga
     3241 cgaaaatgct ccttctgatt tccctcactc aattgatcat ctttctcttt tttgagcgat
     3301 tcaatctttt gctcaaaact tttttgctgg tagatcttaa aagcaataaa tccgcctaat
     3361 aggcaaatag aaattgctaa aataccaatc aaagccaata atacatgtct cgttttcccg
     3421 tgtctgccac gtcctagtct acttttattc atatttatat cataacaaat ttgagcaata
     3481 atttcaatga tacaatttgg caatccacaa ttcctctagc aagccaaagc atcttacatc
     3541 aactccaaca tccgtatctg tagagattat gtagttttct atctatggaa tttctagtag
     3601 attgaagcta gaatagtaga cctatacttc taaaacattg ttagaaatcg atttgactgt
     3661 cctgatcgat ttgtcctgtt ctcatttcat tttactataa aactatgaaa agctaggtca
     3721 tctgagaagt aattactatc gacaattgcg atagcgtata ctagtgcgat ttgtttgtag
     3781 ctctggtggg tatcaccttc acgttggcga attttttcta ggttttgatt aacctgactg
     3841 cacagataag gccataggcg attgatgaaa aaattctgat gatgaacctg aatttcgatg
     3901 ataacttgga ttccattatc taactccgcc aagacgtcta tactagtata gaaatcttac
     3961 gctgagtagg gcatggaagg caagacgtga atgttacttc cctccaaaat cgtcacattt
     4021 tttgctggca agtccagcat atcgcggata aattgacaaa tcattagttg ggctgatacc
     4081 tagatgtctg agaatcctcc ttttcctcct tctattatag cacattttta aatctaggtt
     4141 tgctagattc tatgcttcta tctatttatt cggaaaagaa agctaatagc ttatttgaaa
     4201 ttttctcctc tctgttggat agtcccgtct atctatgtta taatgaaaga aggatttaaa
     4261 atcggaaaag gagtatttat gctaaaatta ggtgtcatcg gaactggcgc tatcagccat
     4321 catttcatag aagcagccca taccagtgga gaataccaac tggtcgcaat ctattctaga
     4381 aaactagaaa ctgcagcaac ctttgcttct cgctatcaga atatccaact attcgatcaa
     4441 ttagaagtct tcttcaaatc ttcttttgat ttagtctata tcgccagtcc aaactcctta
     4501 cattttgctc aggcaaaagc tgccttgtct gctggtaaac atgttattct cgaaaagcca
     4561 gctgtctctc aaccacaaga atggtttgat ttgattcaaa cagctgaaaa aaataactgt
     4621 tttatctttg aagcagctcg taattaccac gagaaagcct ttactactat caaaaacttt
     4681 ttagcagata agcaagtgtt aggagcagat ttcaactatg ccaaatactc ttccaagatg
     4741 cctgacttgt tggctggaca aacaccaaat gtcttttcag accgttttgc tggtggagcc
     4801 ctcatggatt tggggattta tcccctctat gctgctgttc gtctttttgg aaaagccaat
     4861 gacgcgactt accatgctca acagcttgac aatagcattg acctaaatgg agatggtatc
     4921 ctcttctacc cagactatca agttcacatc aaggctggaa aaaacatcac ttccaatctt
     4981 ccttgcgaga tttatacaac agatggaacc ttgactctca acacgattga gcatattcgc
     5041 tcagctattt ttaccgacca ccaaggaaat caagtccagc ttcctatcca acaggctcct
     5101 catacgatga ctgaggaagt cgctgcattt gcacacatga tccagcaacc agatctgaat
     5161 ctctaccaaa cttggctgta tgatgcaggc tctgttcatg agctactata taccatgcgc
     5221 cagactgctg gcattagatt tgaggcagaa aaatgaaaac caaactaccg actgaatggc
     5281 aagaactgag tgaccaactc ggtttccaag aattcacccc cattcaaact caactatttg
     5341 agccccttct tgctggagaa aacctcctag gagtgagcca aaccggaact ggtaagaccc
     5401 tagcgtatct cctaccaagt cttctcagac tacaaaagaa aaaagcccaa caactcttga
     5461 ttctagcacc aaatacggaa cttgctggac agatttttga tgtatgtaaa acgtgggcag
     5521 aagctatcgg attgactgct caactcttcc tttcaggttc aagtcagaaa cgacaaattg
     5581 aacgtcttaa aaaggggcca gaaattctga ttggaactcc tggccgtatc tttgaactca
     5641 ttaaacttaa aaaaatcaag atgatgaatg tggaaaccat catcctggat gaatttgacc
     5701 aattactaga tgattctcag attcactttg tagaaaaaat cactcactac gcacctcgtg
     5761 accaccaact ggtctacatg agtgccacga ccaagtttga ccaagaaaag attgtgccga
     5821 atacacgcac tattgatctc tctgatcaaa aattggacaa tatccagcat ttctacatgc
     5881 aggtagacca acgtcatcga gtggatatgc tacgaaaact ggctcatgta gaggatttcc
     5941 gtggtctagt cttcttcaat agcctgtcag accttgggaa tgccgaggaa aaactacagt
     6001 atcgtgatat attggctgtt tccctcgcta gtgatgttaa tgttaaattt agaaaaatca
     6061 tcttagaaaa gtttaaagat aaccaactaa ccctgcttct tgcaactgac cttttggctc
     6121 gtggaattga tatcgatagc ctagaatgtg tcgtaaactt tgatattcct agagatagcg
     6181 aaacctacac acaccgtgct ggccgtactg gtcgtatggg caaggaaggt tatgttatca
     6241 ctctcgtcac tcatccagaa gaaatcaaaa aacttaaaaa gtttgcaagt atccgagaaa
     6301 ttgtcctaaa aaaccaagaa ctctatatca aataaagccc cattgctcta tacacatcca
     6361 gtgtatggag cttttttata agcaattcaa ttttatactc atctgttttc aaaaagcatt
     6421 ctagtccatc tccgattaac gatggacttt atcacctcct tctccaatcc ttgtatgaca
     6481 tcttggagtt gattcatgac atcttccaaa gttcgaaagg ctttattctt aaatccacgt
     6541 ttacgaatct ctttccacac ttgttcaatg ggttcatctc tggtgtgtat ggaggaataa
     6601 aggtaaaacc aatattagtc ggaatcttta aggtacttga tttatgccat atagcattgt
     6661 ccataacgag taaaagataa tcatctggat aagcttgtga aagctcttct aaaaaggcgt
     6721 tcatccactc agtattacat ccaccagcta ttaagaaaaa tgattcgcct gtatgggcat
     6781 caacagctcc ataacaatag cgaaattctc gtatatagtg actatggaca tgtggaccta
     6841 ctcctattgg agaccaacaa gatcccagtt tactgattct accgaaacca gcctcatctt
     6901 ggtacatcaa gcgaacctta tgaaaaggtc tactggtttt aaagcgcttt cttgtgtact
     6961 gcccccaaac agttagac
//
LOCUS       DAVHEW010000100         6740 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVHEW010000100 DAVHEW010000000
VERSION     DAVHEW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.100"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            38..2311
                     /locus_tag="VVL53_001839"
     CDS             38..2311
                     /locus_tag="VVL53_001839"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU4183959.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            2328..2798
                     /locus_tag="VVL53_001840"
     CDS             2328..2798
                     /locus_tag="VVL53_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU4183960.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(3304..6654)
                     /locus_tag="VVL53_001841"
     CDS             complement(3304..6654)
                     /locus_tag="VVL53_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU4183961.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         2091 a         1462 c         1143 g         2044 t
ORIGIN      
        1 tataataacc ttaactgtca ttctatatgg aggttctatg cgttttaatc aatatagtta
       61 tatcaatttt ccaaaagaaa atgtcttatc tgaactaaaa aaatgtggct ttgatttaca
      121 aaatacagcc aatcacaaag actcacttga aaccttttta cgtcgtttct ttttcactta
      181 tcaagacacc aactacccac tttctatcct agcagctgat aaaaaaacag acctgctgac
      241 attttttcaa tcagaagatg aactgacagc agatattttt tatactgttg cttttcaact
      301 tttaggcttt tcttatttgg ttgacttcga agatagtgac gtttttcgta aagaaactgg
      361 atttcccatt atatatggtg acttgattga aaatctctat cagttactca atactcgcac
      421 caaaaaagga aatactctta tcgaccaact tgttagtgat ggtcttattc cggaggataa
      481 tgactaccac tactttaacg gcaagagttt ggctactttt tctaaccagg atgtcattcg
      541 tgaagtcgtt tacgttgagt ctcgtgtcga tactgaccaa aaagggctat cagacctagt
      601 aaaggttagc attattcgcc ctcgttttga tggaaaaatc cctgccatca tgacagccag
      661 cccttatcat cagggaacca atgacaaggc tagtgacaag gctctctaca agatggaggg
      721 cgagcttgag gttaagcttc ctcacaagat tgagctagag aaacctcaac taaatctcgt
      781 ccaaccccaa ggtaaagctg agcttatagc agaggctgag gaaaagctaa ctcacatcaa
      841 ctctagctat acactaaacg actacttcct tccgagaggc tttgccaatc tctatgtctc
      901 aggtgttggt accaaagact cgacaggttt catgactaat ggagactacc agcaaatcga
      961 ggcttataaa aatgtcatcg attggcttaa cggtcgttgc cgtgccttta ctgatcacac
     1021 gcgccagcgt caagtcaagg ctgattggtc aaacggaaaa gttgctacaa caggactttc
     1081 ctatctaggt accatgtcca atggtcttgc gactacagga gtcgatggtt tagaagttat
     1141 cattgccgag gcaggaattt catcatggta caactactac cgtgaaaacg gtctggtgac
     1201 tagcccaggt ggttatccag gtgaggactt tgactccctt gctgagttaa cctattctcg
     1261 taatctctta gctggcgact atatccgtgg caatgaagct caccaagctg acttagaaaa
     1321 agtgaaagct cagctagacc gaaaaactgg ggactacaat cagttttggc atgaccgcaa
     1381 ttatctgctc aatgcccata aagtaaaggc agaggttgtc tttactcatg gttctcagga
     1441 ttggaatgtc aaaccacttc atgtttacca gatgttccat gctcttccta ctcatataca
     1501 caagcatctc tttttccata atggtgccca tgtttacatg aacaattggc aatcaattga
     1561 cttccgtgag tccataaatg ccttattgac caagaaatta ctaggacagg aaacagactt
     1621 tcaacttcct actgttatct ggcaggacaa tacagctcca cagacttggt tatcacttga
     1681 taacttcggt gggcaagaaa actgtgaaac cttctcactt ggtcaagaag agcaagctat
     1741 tcaaaaccag tacccagata aggattttga gcgctatggt aagacatacc aaaccttcaa
     1801 tacagagctc tatcaaggga aagccaatca gattactatt aaccttcctg tgactaaaga
     1861 tctccaccta aacggtcgcg ctcagctcaa tcttcgtatc aaatccagta caaacaaggg
     1921 gctcttatct gcccaactgc tagaatttgg gcaaaagaaa tacctacaac cttatccagc
     1981 tattttaagt gctagaacca ttgacaacgg tcgctatcac atgttggaaa atctctgtga
     2041 attgccattt agaccagagg cacaacgagt cgtgacaaaa ggttacctta atttacaaaa
     2101 tagaaatgat ttactgttag tagaggatat tactgcagat gaatggatgg acgttcaatt
     2161 tgaactgcag ccaactattt acaagctaaa agaaggagac actctccgtt tagtcctcta
     2221 tactactgac tttgaaatca ccatccgtga caatacagac taccacttga ctgtcgacct
     2281 cgctcagtcc atgcttacct taccttgcta aaaggagtta cattattatg aataaatcag
     2341 aacaccgcca ccaacttata cgcgctctta tcacaaaaaa caagattcat acacaggctg
     2401 agttgcaagc ccttcttgct gagaacgaca ttcaagtaac ccaggcaacc ctctcacgcg
     2461 acatcaaaaa tatgaaccta tcaaaagtcc gcgaagaaga tagcgcttat tatgttctta
     2521 acaatggttc catctcaaaa tgggaaaaac gtctcgaact ctacatggaa gacgcccttg
     2581 tctggatgcg cccagttcaa caccaagtcc tactaaaaac ccttcctgga ctggctcaat
     2641 cctttggttc tatcattgat actttgagct tccctgacgc tatcgctacc ctttgtggta
     2701 atgatgtctg tcttatcatc tgtgaagatg cagatactgc tcaaaagtgc tttgaagaac
     2761 tgaaaaaatt cgccccacca tttttctttg aagaataata aatgactaca actaacaatt
     2821 atgcttttaa aaaacctggt agaaagtctt taacatcaga aaacgcatag tatcaagtgt
     2881 tgaaaaccct tgatactatg cgttttgctt tatcctagga agatttatac tcttcgaaaa
     2941 tctcttcaat ccgcgtcagc ttccatctgc aacctcaaaa cagtgttttg agcagcctgc
     3001 agctagtttc ctagtttgct ctttgatttt cattgagtat tatttcattt tctcctgcaa
     3061 ttgaattctt gctcggcttt ttgttttcta tttctttaaa atcaaagtag ctcttttgtt
     3121 aataactcga tcaacaaaca tcgtggtaca agtatctact ttgaaattta tcaaccactt
     3181 aacaactgat actgtattct aggaaaacga tgacattctt cctaataaaa cttctcatat
     3241 atagcataaa tttctactct ttttaatttt aaatgagtct cagcatggca atcactttag
     3301 aatttaagcc gcttctccag ctcgattatt gataagttca atgatttgaa caatattacg
     3361 aagtactggt agttggtgtt ggaagagtag ttgaacagaa ccataagatt taaacggctc
     3421 ttgcgttaac actttcgtct ctaaaaaacc attttctaca atgtagtcta caatcaattt
     3481 tacaaatgaa atctgcctgg cattaagatt ctcatccgac aaaaatttag aaaaaatacg
     3541 attggcagac tctctatcta agccaataat ctcacgaacc aatctcggaa ttgccttatt
     3601 ttcataatga ctttggtagt ctgctttact acctaatttt tcccaaagca atttttcaag
     3661 tgcaagcata tcatcagatg tcaacttttt attatggtat agcttagaaa tggactcctc
     3721 atccagatga gttttcaaat aatgctcaac tttttcattg taagaccgaa gatcgttgac
     3781 ctgcaaaaat gctgtggtct cgtgaacagt ggagagtata cgatcttcaa agttaacata
     3841 gtagggttta cggtctgttt tatccaaaaa ctgtaataaa tctcgaatag caagacgaat
     3901 tttttccaaa tcagacaagt taacttcttt ccaaaattca ggctcctgta ctttcctgat
     3961 aatttcagcc tgctcaaaaa cctgcgggat attgccaata gcagaaagag ctctagccgt
     4021 cttcatcact tgggaaatat gaacagtgga agatttagct gtcagttgcc ccaactgaat
     4081 atgaagcaac cacaaatcaa atctcctcgc catctcatct tctttatctt catcaaataa
     4141 aagcggagag agattttttt gaatggtttc acttgtaaca gcagttagat tctgccaact
     4201 ttccaatttc ctatagctat aaactgtatc taaaaccata cgaaccctga agtccaactc
     4261 atttaagctc tctatacgac cttgaagttc cgagacaagc tgctgacggt atgctctcgc
     4321 aaactgatct tcttggtatt ggagtccctg aagttctcga atcaagtcca ctttgatatt
     4381 aaataaacgc tgagtcagcg aaacaatgtg acgaccctct ccatctcttg gatctgcacg
     4441 aaaataatca aaattgtccc cataatcaaa taccaagaag ttttccttat cctgctcagg
     4501 tccaaataaa tctttacata gacgggttcc tcgaccaatc atctgccaaa acttagtttt
     4561 agagcgtact ttcttgaaga agactaaatt aacaacctct ggtacatcaa tacctgtatc
     4621 taacatatcg acagaaatcg caatttgagg atacttctcc ttaattttaa aatcatcaat
     4681 caaggtctga taatgcttaa tactataatc aatcacctga gcatagtcgc tccctttttc
     4741 aggatagcgg ttgttaaaaa tacctctgat atattccgca tgatcatgat ttttagcaaa
     4801 aataatagtt ttaccaattt catcacccga ggctgtctga attcctcttg tcatgagttc
     4861 atttaaaaca atttctactg tacttttatt gaaaataaag gaattaaata cactcccatc
     4921 aatatctttt tcacagctat tgtcttcaaa tttgctatca aaatgttcct tttcttcttc
     4981 ggacaaatca tcataatgta gaccatccgt aggtagtttc agtttggttt cgatagaatg
     5041 ataggctact aaatatccgt ctttaacagc ctcttccaaa tcatatgcat atgttggaac
     5101 cccattctcc aaattaaaga atccataggt gtttttatct aaatcttgac gcggagtagc
     5161 tgttaagcct acaattcttg catcaaaata atcaaaaatg gacttgtatt tctgataaat
     5221 agaacggtga gattcgtcaa ttatgataag gtcaaaatgc ccaacagtga aagggcgttg
     5281 atttacttct tcttgaccac taatcgctcc aatcatggtc ggataagttg aaaagaccat
     5341 gcgacttgat tgagctcctt ctttatcttc taagaagtta caaacggaaa gatctgggag
     5401 taattttcta aacgaatcat aggcttgctt aaccaaggaa gttctatcgg ctaagaagag
     5461 aacgtttttt acccagttat gacgtgataa gatatcaact agagaaactg ctgtacgagt
     5521 tttcccagcc ccagttgcca taaccaaaag tgcctgtcta cgatgattag agaaagcttc
     5581 acaaacgctt gcaatggcat gtttttggta gtgacgcccg gaaatatcgt ccctaatttt
     5641 actagaaata tcctcaagtg gtttttgaag atgacgtcta tccatcacta attgcaattc
     5701 ttcttgagag taaaagcctg caatctggcg gcggttcgga ccatcaagta tatagtgctt
     5761 caagccattt gtaataaagc aaattggctg atagccgata tgtttctcaa gagcttcagc
     5821 atattctttg acctgtactt cccctacttc tggattgaca gaggcttttt tagcctctac
     5881 aatcgctaaa attttcccat ttttaccata aagtacatag tcacagtaac caattcctga
     5941 accgtgcttg agaccatcaa cggctatctc aacacgacag ttttcttcaa atatccatcc
     6001 tgctaaacgg agatcgatat caataaagag ctttcttgtc tctgcctcag ataattgatc
     6061 aatatggaaa ggcacatctt gccgttgcgc tgcagtctca cgtttttcag tataagtctc
     6121 ttgaacttct acggactgag cagccatctg ttcatgaaaa tcaggcagtt ctggtaaact
     6181 gtcttgtaac tttatcatag attgtggagt ttctcggtat ttgatgtttg ctgaaagtgg
     6241 taagcacttc tcatcaaaat aacgctccac aaactcattg ctgtaacaat aatcgataaa
     6301 attaacaaac tgatacaaat gatgcaaagc taaaatcgct tctcgttcct taatttcacc
     6361 accatgagca gcatggtttc cccaccgaat caacagaact atctgcttgt ggagttcaga
     6421 atctacgata tccctaaaat catcatccca tactaaagaa gatagagtag cacgataggg
     6481 agcttctaaa tatgaatcgt gactatatat ccaatggaca gcctgctcta aagcacgacg
     6541 cgccataaag gcagtagcca cagttgatgt agcaatcata ttctcagctt caatgcaagg
     6601 ttttgaaaaa gattcatatt cgtcattttt taaaagaaag ctaaaatttc ccattttttt
     6661 ctcactttaa acaaatagtt tagctatatt atactaaatt tcaagctatt tctccacact
     6721 tttatagcta tctcacaagc
//
LOCUS       DAVHEW010000101         6356 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVHEW010000101 DAVHEW010000000
VERSION     DAVHEW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.101"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            86..1261
                     /gene="mutY"
                     /locus_tag="VVL53_001842"
     CDS             86..1261
                     /gene="mutY"
                     /locus_tag="VVL53_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU4183962.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQVYAQANLDSSQD"
     gene            1317..2021
                     /locus_tag="VVL53_001843"
     CDS             1317..2021
                     /locus_tag="VVL53_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183963.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="VVL53_001844"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="VVL53_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU4183964.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQV
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="VVL53_001845"
     CDS             3365..4174
                     /locus_tag="VVL53_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4183965.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /locus_tag="VVL53_001846"
     CDS             4265..4507
                     /locus_tag="VVL53_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU4183966.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="VVL53_001847"
     CDS             4509..4763
                     /locus_tag="VVL53_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU4183967.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4838..>6356
                     /locus_tag="VVL53_001848"
     CDS             4838..>6356
                     /locus_tag="VVL53_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEU4183968.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEA"
BASE COUNT         2037 a         1048 c         1414 g         1857 t
ORIGIN      
        1 agatagaaaa gttatatact atctattgaa aatattataa atcagataca aagacaggtg
       61 aaaccttctg tgttatacta gagatatgtt agatttgaaa gaatacggta tcgtcatgtg
      121 gccggaggag aaagtcattt ctttccgtga gaaacttctc gcttggtatg atgaaaacaa
      181 aagagatttg ccttggagga gaagtaaaaa tccttatcac atctgggtat ctgaaatcat
      241 gcttcagcag accagggtgg atacagttat cccttactac gaaagatttt tggactggtt
      301 tccaactgtc gaaagtctgg caactgcgcc tgaggagagt ttactgaaag cttgggaggg
      361 cttgggctat tattctcgag ttcgcaatat gcaggctgca gcccagcaga ttatgactga
      421 ctttggtggc caatttccaa atacctatga aggaatttcc agcttgaaag ggattggacc
      481 ttacacagca ggagccattt ccagtattgc ttttaacttg cctgagccag ctgtagatgg
      541 taatgtcatg cgggtcttgg cgcgtctgtt tgaagtcaac cacgatattg ggattccaag
      601 taatcgaaaa atttttcagg caatgatgga gatcttgatt aatccggatc gtccgggtga
      661 ttttaatcaa gccttgatgg acttagggtc tgatattgag tctcctgtaa atcccagacc
      721 tgaagaaagc ccagtcaagg actttagtgc ggcatatcag aatggaacca tggaccgtta
      781 tccaattaaa tctcctaaga aaaagccggt tcctatttat cttaaagcct tagttgtgaa
      841 aaattctcaa ggacaatttt tacttgaaaa aaatgaaagc gaaaagctct tggcaggttt
      901 ttggcatttc ccctttatag aagttgataa cttttcgcaa gaagagcagt ttgacctctt
      961 tcatcaggtt gcggaagaaa gtgtgaactt tggtcccagt ccagaagaga gtttccagca
     1021 ggactatgac ctagatgttg attggcttga tgtttgtttt gatacagtcc agcatgtttt
     1081 tagtcatcga aaatggcatg tacagattgt agcaggtcag gtgagtgact tccatgattt
     1141 ttcagatagg gaagttcgct ggctttcacc agaagaattc aagaattacc cacttgccaa
     1201 accccaacaa aaaatctggc aggtttatgc acaagccaac ttagacagta gccaagacta
     1261 gctattgtgt cttctgacta tttttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacaaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaagctct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg tgtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aagtcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaggtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 actaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cacaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtactccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tcctcttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggttatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgactcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaacaa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact gaacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgttttat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaac caatgttttg gaagacccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt cgttgctact ctagaagttg
     4741 gtcatcggag agatatttat tgaattactt ttttcaagta aattttcatt ctttaaaata
     4801 tgatataata gggcttacaa tataagagag gttgctgatg acaatagata ttagtgaaga
     4861 aagtttggct aaagaatcgg ctgacttatt aaaaattctt ttaaaagatc gaacgacaaa
     4921 gaaatcaatt gtttgggcta cgcattctta tgaattgtta ggaaagggat ttgctccaag
     4981 tgatcgcatc aatccaagta aggttactgg aaactttgcc aacttgattc agcccagatc
     5041 tgaaaaatcc aaatatgaac aaaaagatcg gacaaaaata agggcagaag tgtttacacc
     5101 aacgtggttg gtcgcaaagc aaaatggata tgtagaatcg aaattaggct cgttaagtct
     5161 tgaggagtat gtggacttac gatggttaga ggtaacttgt ggtgaagcac cttatatggt
     5221 aacgagatat gataccgtaa ctggtgagga aattccatta tctgagcgag ttggttttgt
     5281 tgatagaaaa ctgcaacgga ttagtcgtga ggtttcagat gaagtcacat tttataaact
     5341 tgtaaaagat gcttatcatg cttcgtatgg ttatgagtat caaggagatt ctctcctttt
     5401 ggcgcgtgag aatcttttgg caacttttga ggactattat ctagctaaga ctggaaatca
     5461 accaacatta gagcaaaaga aagaaattgc gactattatc tcttacaatg tctttcagat
     5521 ggatggattg aaaaaaagta gtccttattc agctaaacaa aagcaatctc aacaattgag
     5581 tttgtttgcg gatgagctag aagtccaaga agtagaagaa tctaaaaccc atatcaaaga
     5641 ctggaaaaag aatagaatga taggttttga gcgcctatct agcgaggaaa gtgaaatgaa
     5701 atttgatgtc gtgataggaa atcctccgta tcaggaaaca ggagaagcca gagatgagcc
     5761 gatatatcac tattttattg atgaagctta taaaatcgca gataaatcta tactaatcac
     5821 cccagcaagg tttttattta aagctggtca aactcctaaa aattggatgg aaaagatgct
     5881 tgaagacaag catttaaaag ttcaattcta tgaacttaaa agcggtaaag tatttacagg
     5941 aacggatatt aagggtggag tagcaataac ttatcgtgat gttgataaag atttgggacc
     6001 aatcggagta tttactattt ttgaaacttt gaatggtatt gtaaaaaaaa tcgaaaaaca
     6061 tccagaattt gaaacttttt ctgatttagt ttatcctcaa ggtatttata gattttcaaa
     6121 taaacttttt tctgattatc caaatgctga aaagctgcaa ggaaaaggga caaaaaataa
     6181 aattgtttcg aaatcactaa ctcagttaga ttttgttttc aaaaacaacg aagaaaaaga
     6241 ccatgttgct ctacttggaa gaattggctc cgaacgtgtt tatcgatata ttaataaaaa
     6301 atatttagat ttaccggaaa cattcgaaaa ttataatgtt tttgtaccag aagcta
//
LOCUS       DAVHEW010000102         6177 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVHEW010000102 DAVHEW010000000
VERSION     DAVHEW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.102"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            90..356
                     /locus_tag="VVL53_001849"
     CDS             90..356
                     /locus_tag="VVL53_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183969.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLGLKRYQTPMDGAKKVFYRPSDVYLFLAIK"
     gene            367..519
                     /locus_tag="VVL53_001850"
     CDS             367..519
                     /locus_tag="VVL53_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183970.1"
                     /translation="MKIVTFKPTKQIDDGFYLPGIDILFVSDKADAKDKEDVILFLSR
                     NGLNKS"
     gene            724..1341
                     /locus_tag="VVL53_001851"
     CDS             724..1341
                     /locus_tag="VVL53_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU4183971.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAEVQHHTITRLIRENKADFEEL
                     GILGFKIHKLDTRGQPKKSYILNEQQATFLITYLKNTETVRQFKLNLVKAFFEMREEL
                     SEIRLQRALEKPKRKTLHDSIETWPNAPKHAHSTMNNLLLKAVTDMNAKQLREERGGY
                     NGIDSLTSEELEQYQAFEDMAIAMIELKMSYQEIKTMMFRSKKIS"
     gene            1376..1828
                     /locus_tag="VVL53_001852"
     CDS             1376..1828
                     /locus_tag="VVL53_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183972.1"
                     /translation="MTETIYENLTRRIDRISAEMREISEENGLRILSLLESQTKSIKD
                     ELSRLLWIELPELNESHKIEAVSKSTTGMFFAPGIFEMDAMRKAFFKRQAKHFFENEA
                     EQQAYIEHAEKEYLEATVTLKDILFNSKNGTQKANKSCLIEKFEEAMQ"
     gene            1825..2271
                     /locus_tag="VVL53_001853"
     CDS             1825..2271
                     /locus_tag="VVL53_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEU4183973.1"
                     /translation="MTLDLDNMTQAEFDEVMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYIKVILN
                     RWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            2283..3167
                     /locus_tag="VVL53_001854"
     CDS             2283..3167
                     /locus_tag="VVL53_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4183974.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EAIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYMQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFGIKYKRYSLNQLKSNM"
     gene            3416..4066
                     /locus_tag="VVL53_001855"
     CDS             3416..4066
                     /locus_tag="VVL53_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183975.1"
                     /translation="MTENKDDKLLKMMEKGFVLYSKNGIIKYIEIPEHGSIKLKAQDG
                     QLVYKEVIIGKVYAEQELLKYKKEGENYSQNTAQLLYQQLEKEKNAELANHESKEEPI
                     TADDAAELSLLSSIKLTVAEMREYLEKYKNKPLALRKLEDIMDNDTTLAYIEIDMEQF
                     SQQQRLEKLVHFLDRKIDYFHGGLLINGDKIDLVQHEMIVEGNSEAMDAELQNYLA"
     gene            4113..4541
                     /locus_tag="VVL53_001856"
     CDS             4113..4541
                     /locus_tag="VVL53_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU4183976.1"
                     /translation="MAGNENDNLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRIKQDEMMDKTTQMLYLASSNAVSVLNDIMMDSKVNPFIRTQAAKAILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGNQRVTRTQGVIK"
     gene            4549..4860
                     /locus_tag="VVL53_001857"
     CDS             4549..4860
                     /locus_tag="VVL53_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183977.1"
                     /translation="MPEFIIVEGNNDLGEFFQIDGELFSDNELLENLKKWREWEVPVI
                     IDDWCNRILNEDETEILYFPTHEDKMNYIRVEKDLEPLYHTSNKIYATISKSEWLELL
                     N"
     gene            4878..5288
                     /locus_tag="VVL53_001858"
     CDS             4878..5288
                     /locus_tag="VVL53_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183978.1"
                     /translation="MTKKKIERISVIHREKILWLKWYFMRDKEQPKYSVLERKMFDAA
                     KNQDMLAYQKYATIKQITDIRVQTSEADILEAVKEVYVYNHMNVIGACQRILFISQSP
                     AYDKLNKWFNIYSDLYFSVVPLPKMGVYHEMVGI"
     gene            5485..5850
                     /locus_tag="VVL53_001859"
     CDS             5485..5850
                     /locus_tag="VVL53_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU4183979.1"
                     /translation="MHNIYFYKDKNGNEPVFDYMRELTSKKGKDSRIKLNKINDYIEL
                     LSQHGTRAGEPYIKHLDAEIWELRPLRDRILFVAWMDGSFVLLHHFMKRTQKTPKREI
                     EQAKRELADLKERGLDNEK"
     gene            5840..6133
                     /locus_tag="VVL53_001860"
     CDS             5840..6133
                     /locus_tag="VVL53_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4183980.1"
                     /translation="MKNNAIGSNWKDVRAELFSKEEILESDMRVAIMSELIEARNEKG
                     ISQKKLEEMSGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEHEQV"
BASE COUNT         2341 a          885 c         1310 g         1641 t
ORIGIN      
        1 aacccctttg taaataatct cctcttattt tatcaaaatt agaggaaaat gacaacttaa
       61 tttataaaaa ggaaaaatgg aggatataaa tggaaattct gtctaaagaa atacagttac
      121 agggcttaca acttcttaaa cagactcttg aaactttagt tgagctagaa aaacaacgat
      181 ctagtaagtt agatttaatt tctcgtaaag aattaatgga tctgctaggt ataagtgcta
      241 caacccttga taactgggag gatcttggtc ttaaacgata tcagactccg atggatggag
      301 ctaagaaagt attctatcgt ccgtcagatg tgtatttatt tttagcaata aaataggagt
      361 tatgaaatga aaattgttac tttcaaacca actaaacaaa tagacgatgg gttttatctg
      421 ccaggtattg acattctatt tgtctcagac aaagcagatg ccaaggataa agaagatgta
      481 attttatttc tttcaagaaa tgggttaaac aagtcttgaa tgatattaga aaataaatct
      541 actattccac ggaggatatc gatacagcga tacaagtcgt tataaaggcc tctaacacgt
      601 ttatagagac aagtggtaaa taatattaac cactagccaa agccgtttta gaggctaaaa
      661 aagaggctag aggaggcaat taaaaagtaa gctaaccaga tttatacagg aggtagaacc
      721 gtaatggaac tagtttacat ggacggtaag aaagagccgt acaccacaag cgagatcatc
      781 gctgaatgtg ctgaagtaca acaccacact atcacgcgct taataagaga aaataaggct
      841 gactttgaag agttgggaat acttggattt aaaatccata aattagatac tagaggacaa
      901 cctaaaaaat cctatattct gaacgagcaa caggctactt ttctgatcac ttatttaaag
      961 aataccgaaa cagtacggca attcaaacta aacctagtca aagcattctt tgaaatgcgt
     1021 gaggaacttt ctgaaattcg tctgcagagg gcgctagaaa aaccgaaaag aaaaaccttg
     1081 catgacagta tagaaacatg gcctaatgca ccaaagcacg cgcatagcac catgaacaac
     1141 ttgctactaa aggcagtaac agacatgaac gctaagcagt taagagaaga gcgtgggggc
     1201 tataacggta tcgatagctt gactagtgag gagttggagc agtaccaggc atttgaggat
     1261 atggcaatag ctatgattga attgaaaatg agttatcagg aaattaagac aatgatgttt
     1321 agaagtaaaa aaataagcta aaaaatcaca ccaacaaaga aacagaggta aaaacatgac
     1381 agaaacaata tatgagaatt taactagacg tatagacaga attagcgcag aaatgcgaga
     1441 aattagcgaa gaaaatggcc taagaatcct ttctttattg gaaagtcaga caaaatccat
     1501 caaagatgag ttgtcccgtt tactttggat tgaacttcct gaattgaatg aaagccataa
     1561 aatcgaagcg gtctctaaaa gcactacggg aatgtttttc gcccctggta tttttgaaat
     1621 ggacgctatg cgaaaagcat tctttaaacg ccaagctaag cacttttttg agaacgaagc
     1681 agagcaacag gcgtatatag agcatgctga aaaggagtat ttagaggcta ctgtaacctt
     1741 aaaagatatt ctttttaact ctaaaaatgg aactcagaaa gcaaataaaa gttgtcttat
     1801 agagaagttt gaggaggcaa tgcaatgaca ctagatttag acaacatgac acaagcagaa
     1861 tttgatgagg taatggctga aatccagcta caaagtccaa acatttttca actgatttct
     1921 gattttgtaa ataaaaaagt aaccagtgtg gaaatagacg acttgttgaa tatgaagcga
     1981 gctgagacag gtaaagcatt gtcaccgtta cagattcaag aattgcagta tatgctagaa
     2041 gattttagcc cagagcttat ccatgaggcg ttaaaagagg ctgtcagtca gggtaaagca
     2101 aactttgcat acatcaaggt aatccttaac cgttggaaac aggacaattt attgacggtg
     2161 gaacttgtta gaaatagcag ggcagcgcgt gaggctaaaa aacagcaaac caatcaacta
     2221 gagcctacca gctatgaaga ttggatacca acacaagaaa aaccatttta ggaggtcaga
     2281 aaatgaaatc attacaatct agtttaggaa atattgagac atttaaacta gacgaattgt
     2341 gtcctaagca tgaaatacag ctaacacaaa tcaaaggaga aagacatgta gtagtggggt
     2401 ggaatgagaa cggagaggca ataaaagaag ttcgctgtat cccaccctat tgtgaacaat
     2461 gccaagaaga acagaaaaag caagatgaag aagatgcgat tgctgataat ttgaatgcta
     2521 aactatatat gcaaacttat aatgtgttga tgagcaataa gagctatgta gcagatgagt
     2581 ttaaaactaa aacttttgat gattttaatg ctgtaacatc agaagaaaag cgatttttag
     2641 aatttgcaaa aggtcaggta caaaaatatc ttgatggcat gaggggaaat acgcttatca
     2701 caggaggtac agggattgga aaaacacttt tatcagttgc tattgccaaa ggtataaatg
     2761 aggggtataa gacaaaagga gaacctaaga gtgtgctttt tgtaagccta accgaaatga
     2821 tcaaacaaat taaagagggg tggaattatg gtaaaggtgc aacacttaca gagtttgaag
     2881 ctactgaact catgaagtct gttgattact taattattga tgatttagga gcaaaaaatg
     2941 ctgttattaa gcctaagagt gactgggagc aggatttact ctttgacgtg ctaaacaatc
     3001 gtgaaaatac catttttaat accaacctag atagtgatga gcttaagata gtttacaatg
     3061 agcggaatta tagccgtatt ctaaaaggac ttgagggtaa cacttttaag gcgtttggca
     3121 tcaaatataa gcggtactca ctcaatcagt taaagagtaa tatgtgacaa tgaattaact
     3181 tacaaaccta aacaaaaata gacacttttc atggggtgag aaatcgccct ttgagaagta
     3241 atagccatgc tttccacgac catataaacc aagtgtggca aactaaccaa tttgaaaagt
     3301 cagccataag gtacacaaaa agggtagtat tttagcatac gaaccctaaa aacctagtga
     3361 aataaataga ctaggggtat tcagattata ataaaatgaa ataaaggaga aaagcatgac
     3421 tgaaaacaag gatgataaat tattaaaaat gatggagaaa ggctttgttt tatactcaaa
     3481 aaatggtata attaagtata ttgaaattcc agagcatggg agtattaagc taaaagctca
     3541 agatgggcaa ttagtttata aggaagtgat tatcgggaaa gtgtatgcag agcaagaact
     3601 tttaaaatat aaaaaagagg gcgaaaacta ttcccaaaat actgctcagc tattgtatca
     3661 gcaactagaa aaagagaaaa atgctgaact agcaaaccat gaatcaaaag aagagcctat
     3721 tacagcagat gatgcagctg aactaagttt attatctagt atcaaattaa cagtggcaga
     3781 aatgagagaa tacctagaaa aatataaaaa taaacctcta gctcttagaa aattagaaga
     3841 tattatggat aatgatacta cccttgctta tatcgaaatt gacatggagc aattcagcca
     3901 acagcaacgc cttgaaaagc tagtacattt ccttgataga aaaattgatt atttccacgg
     3961 tggtttactt attaacggag ataagattga tttagtacag catgaaatga ttgttgaggg
     4021 caattcagag gcaatggatg cagaattgca gaattatcta gcatagggaa ataaaggggc
     4081 aaccctttat tttgataatg aggaggtaat agatggcagg aaatgaaaat gataacctta
     4141 cgtccaagca aattaaattc atagatgcca tgcttaccga gccaacgata gacaaagcat
     4201 gtcaaaaagc aggggtatca cgcgcaacag gtcataagta tctaaaagtt gcagcggtta
     4261 aaaagacatt gaggataaag caagatgaga tgatggataa aacaactcaa atgctttatt
     4321 tagcatcatc taacgctgtt tctgtactca atgatattat gatggacagt aaggtcaacc
     4381 cgtttataag aactcaagca gcaaaggcta tacttgaaca atcttataag acccatgaaa
     4441 tttttggagt agtaaggcag attgaagaat tgaggttgga aattgaggaa gtatctaaag
     4501 gaaatcaaag agttacaaga actcaaggag ttattaagta atagaaatat gccagagttt
     4561 attattgttg aaggaaacaa tgacttaggg gaatttttcc aaatagatgg cgagctattt
     4621 agtgataatg aacttttaga aaaccttaaa aaatggcgtg agtgggaagt gccagttatt
     4681 attgatgatt ggtgcaatcg tatcctaaac gaggatgaaa cggaaatact atactttcct
     4741 actcatgagg ataagatgaa ctatattagg gtggaaaaag atttagaacc tttgtatcat
     4801 acatcgaaca agatctatgc aactatttct aaaagtgagt ggttagaatt gttaaattga
     4861 tgatttagga ggtagcaatg acaaagaaga aaattgagcg tatttctgta atacaccgag
     4921 aaaagatttt atggctcaag tggtatttca tgcgagataa agaacaacct aagtatagtg
     4981 tccttgagcg taaaatgttt gatgctgcta aaaatcaaga tatgctagct tatcaaaaat
     5041 acgcaactat caagcagata acagatatta gggtacaaac aagtgaggct gacattttag
     5101 aggctgtaaa agaggtttat gtgtacaatc acatgaatgt tatcggagct tgtcagcgga
     5161 tattatttat cagtcaatca ccagcttatg ataagttaaa taagtggttt aatatctatt
     5221 ctgatttgta ttttagcgtt gtacccttgc ccaaaatggg ggtatatcat gagatggtag
     5281 gtatctagtt gattgtgtgg tataatattc taaagcacaa gggatagtat agaaggagta
     5341 caccttgata gaggtagcca cggtgcgaat ccgtgtcatt gtgctagcat ctaggaaact
     5401 aggtgctttt tattttaata acttaaaagt atttacaaac aataactttt aggttataat
     5461 tgttattagg aagaggtgtt aatagtgcat aatatctatt tttacaagga taaaaacggc
     5521 aatgagcctg tttttgatta tatgcgagag cttaccagta aaaaagggaa agatagcaga
     5581 attaagctta ataaaattaa tgattatatt gagttgttaa gccaacatgg aacacgcgca
     5641 ggcgaaccat atattaagca tttagatgct gaaatttggg agctgagacc acttagagat
     5701 agaattttat ttgttgcttg gatggatggt agttttgttt tactgcatca ttttatgaaa
     5761 aggacacaga aaacacccaa aagagagatt gagcaagcta aacgtgaact tgcagattta
     5821 aaagaaagag gtttagacaa tgaaaaataa tgctattggt agtaactgga aagatgtaag
     5881 agctgaatta ttcagcaaag aggaaatttt ggaaagtgat atgcgcgtgg ctatcatgag
     5941 tgagcttatc gaggctagaa atgaaaaggg tattagtcag aagaaactag aggagatgag
     6001 cggtgttagt cagccagtta tcgctagaat ggaaacagga aagacaagcc cacaacttga
     6061 tacagtatta aaagtgttgg caagtcttgg taagacctta gctgttgtac cattagagca
     6121 tgagcaggtt tagaaagttt aggtgatatt ttgacagcag aaattggaat attaaat
//
LOCUS       DAVHEW010000103         6156 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVHEW010000103 DAVHEW010000000
VERSION     DAVHEW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.103"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            177..1946
                     /locus_tag="VVL53_001861"
     CDS             177..1946
                     /locus_tag="VVL53_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU4183981.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1970..3925
                     /locus_tag="VVL53_001862"
     CDS             1970..3925
                     /locus_tag="VVL53_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU4183982.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3927..4364
                     /locus_tag="VVL53_001863"
     CDS             3927..4364
                     /locus_tag="VVL53_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4183983.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4426..5562
                     /locus_tag="VVL53_001864"
     CDS             4426..5562
                     /locus_tag="VVL53_001864"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU4183984.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQRTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1891 a         1034 c         1256 g         1975 t
ORIGIN      
        1 gtctaacttt tggggtgtag ttcatgtaca cctgatatga tgcgttttat aatttttaag
       61 cctttttgcc cagcctcgtc aaaagtaatg ttttgacaca aaatctgtga caaaacttta
      121 gttttaaagg tttttaactt tgtatatact agttttaaga aaaggaggat gatctaatgg
      181 aagaaaaagt atcattgaaa gtcagggttc aaaaactagg gacatcgctt tcaaatatgg
      241 ttatgcccaa tattggagca tttattgctt ggggagtatt gactgccctc tttatcgctg
      301 atggctatct gccaaatgaa cagttagcta ctgttgttgg tcctatgtta acgtatttat
      361 tgccaatcct gattggttac acaggtggat atatgatcca tggccaacgt ggtgccgttg
      421 taggagctat tgctactgtt ggtgcaatca caggttctag tgttcctatg tttatcggag
      481 ctatggtaat gggcccactg ggaggatgga ctatcaagaa atttgatgag aagttccaag
      541 aaaaaattcg tcccggattt gaaatgttag ttaataactt ctcagctggt ctcgttggtt
      601 ttgcattatt gcttttggct ttctacgcaa tcggtccagt cgtatcgact cttactggag
      661 ctgttgggaa tggtgttgag gctattgtca atgctcgcct ccttcctatg gctaatatta
      721 tcatcgaacc ggctaaagtc cttttcctca ataatgccct caatcatggc atttttactc
      781 ctctgggagt agaacaggta gctcaagctg gtaagtcaat tctcttccta ttggaagcta
      841 atcctggacc aggcctggga attctattag cttatgctgt attcggtaaa ggttctgcta
      901 aatcttcttc ttggggggca atggttattc atttcttcgg agggattcat gaaatttact
      961 ttccttatgt tatgatgaag cctactctat ttttagctgc tatggcagga ggtatctctg
     1021 gaacttttac ttttcaactc ttagacgctg gtcttaaatc tccagcttca ccaggttcta
     1081 ttattgcgat tatggctacg gcgccaaaag gtgtttggcc ccatctaaat gttcttctag
     1141 gtgttttagt agcagcagtt gtttctttcc ttgtagcagc ccttattctt catgcagaca
     1201 agtcaactga ggattcgctc gaagctgctc aggcggctac ccaagcagct aaggctcagt
     1261 ctaaaggtca gttagtatca acttctgttg atgcagttgt ttcgacagac tcagtggaaa
     1321 aaatcatttt cgcctgcgat gctggtatgg gaagctctgc tatgggagct agtattcttc
     1381 gagataaggt taaaaaagca ggtctagaga ttccagtatc taatcaggca atctcaaatt
     1441 tgcttgatac accaaaaaca ttaattgtta ctcaggaaga actgacacca agagctaaag
     1501 acaagagtcc aagtgctatt catgtttctg ttgataattt cttagcgtcc cctcgttatg
     1561 atgaaattgt agcttcatta acaggagctt ttccaatagc agaaattgaa ggagatatac
     1621 caacttcagc accagtagat agtcaggaaa ttgaccttaa ccatattgat gctgtagtag
     1681 ttgcttatgg taaagcacag ggaactgcaa ctatgggctg tgaaacgatt cgggctatct
     1741 ttagaaacaa gaatattcgt attccagttt ctactgccaa aatttcagaa ttaggtgaat
     1801 ttaattctaa aaacataatg attgtaacaa ctatttcttt acaggcagaa gtgcagcaag
     1861 cagcaccgaa ttctcaattt cttattgtgg atagtttagt aacaacacca gaatatgaca
     1921 aaatggctgc tagaatgtac aaatagaact agaggtttct aaattacgaa tgctattaac
     1981 caaacgagaa gaacaattat tgaaggcttt cctacatgta gggaagcttt caatgcaaga
     2041 tatgactgaa atcttacagg tttcatctag aacaatttat cgaactttat cagatttgac
     2101 agatagcatg gagcaatatg gaatcgaaat aacgaagcat gggaaatact atattttgac
     2161 tggagagttg gatgatttgc cgacagaact tgaagtgtta gttgagtata gtccccaaga
     2221 aagacaagag ttgattacct atcgccttct gactgagagc ggttttgtca ccaatgaagc
     2281 attgcaagag tgcacgaaag tcagtaatgt aactattatt caggatattt cagatattga
     2341 taagcgtctt ttagactttg atctgaaaat tgaacgacaa aaaggttatc ggatttctgg
     2401 tgattcagtt ggtaagagaa gatttttggc tattttactg acaaactgta tctcagtagc
     2461 agatttttca accggtaatt ttgggagctt tgatatttta gaagcagata gaactaggct
     2521 ggccagtcag attgttaata agcaactgtc aggttttcca gatatggatg ctaggatgaa
     2581 gatgtttttt gcgatcttgt tatctcttat aggtcaggag caaaacattg aaaattcacc
     2641 taatactagt aagcaggctt tggaaatttc tcaaaaaatt tttcaaactt actctaagca
     2701 gactgcacaa ttttatagta ttcaggaaat tatctatttt gcgagcatct tggatgaatt
     2761 aatcattaaa cgtcaggaca atccgctctt tacggagaaa tttgatggtg aatttttcta
     2821 caatatttca aatctgattg atacggtttc catgtatacc aagattgact tttttaagga
     2881 caaggtttta ttcaattttc ttttccatca tattcggctc agtttaggcg tccctatcct
     2941 ttttcagggt gaaaatttgc cagaatctgt ccagatttta gttgaaagga ataaatttct
     3001 ttatacagtc atcagtcttt tagtgaatga tatttttccg aaatatcttc atacagagta
     3061 tgagtatggc atgattgccc tacattttat ctctagctta ggccgtagtc cagagattta
     3121 tccagtccgt gttttgcttt taacggatga acgtcgggtc actagagatt tattagtcag
     3181 taaaattaag agtgttgctc cttttgtaga gttgatagat attcagtctc tagtagatta
     3241 ccacagtatt gatctcagtc agtatgatta tattttatct accaagccgc tgactaatca
     3301 ggaaatcgat gtaatttcta gttttccaac cgtcaaagaa ttgcttgaat tacaggaacg
     3361 acttcagtat gtacaggcac atcgtacaat tgtcgcgcgt gatgctatcg ctccagagaa
     3421 aagttatgac ttgcaagatt atttaatatc tagtagtcag cttttgagtc aattcgagtt
     3481 ggttcaattg gagaataatc aatcatttga gcacacggta gaacaaatca tccaatatca
     3541 gaagaatgtg agtgacagag attacctaac aagaaaattg ttatctcact tccagaatag
     3601 tcctatggct attcctaata ctggtctggt gcttttacat agtcagtcta gcaaagtaac
     3661 aacaaatagt tttactatgt ttgaactcaa actacctatc tccgcattgt caatgaaacg
     3721 agaggaagaa gaggtcaaaa ggtgtctgct aatgctaatg tctaaagaag ctagcgagga
     3781 agctagagat ttaatgacag ctatcagtca gtcgattatt gaaaatcatc tttatacaga
     3841 gatttacaag acgggaaatc aatccattat ttatcagatg ctaaatacta tttttaacga
     3901 aaaaattaag aaattggaga actaatatga aacttgaaaa acatttgatt aagcttaata
     3961 aacaattttc taacaaggag gaagctattt gttattgtgg gcaagttctt tatgagggtg
     4021 gatatgttaa tgaagactat attgaagcca tgattgagcg agataaagag ttatctgttt
     4081 acatgggtaa ctttatcgcc ataccgcatg gaacagatgc agcaaaaaag gatgtcctca
     4141 agtctggtat tacagtcgtt caagtcccta gaggggttga ttttgggaat gtatctaacc
     4201 ctcaagtggc aacggttctt tttggtattg ctggtattgg taatgaacac ttagaaatta
     4261 ttcagaaaat ttctatcttc tgtgcagatg tagataatgt tcttaaacta gcagatgctc
     4321 agtcaaaaga ggaagtattg cgcttatttg atgctgttga ataattgaat ttcgtcattt
     4381 gtcatctagt atatatgtcc ctcaaatagg aaaaggagaa attgaatgaa acattctgtt
     4441 cattttggtg ccggtaatat cggtcgtggt tttataggtg aaattctatt taaaaatggt
     4501 ttccatattg attttgtgga tgtcaataat cagataattc atgctctaaa tgaaaagggc
     4561 aagtatgaaa ttgaaattgc acagaaagga cagtctcgta tagaagtaac taatgtggct
     4621 ggaattaata gcaaagaaca tcctgagcaa gtcattgaag cgattcaaag gacggatatt
     4681 attactactg caatcggacc taatatactc ccttttatcg ccgaacttct agccaaagga
     4741 atcgaagctc gccgagttgc aggaaataca caggcattgg atgttatggc ctgtgaaaat
     4801 atgattggcg ggtctcaatt tctttatcaa gaagtcaaga aatatttaag tccggaaggt
     4861 ttgacatttg ctgataacta cataggtttt ccaaatgctg cagtagacag gattgttcca
     4921 gcacaaagtc acgaagattc cctttttgtt gtggtcgagc cctttaatga atgggtcgtg
     4981 gaaaccaagc gtcttaaaaa tccagattta cgtctagaag atgtgcatta tgaagaagat
     5041 ttagaaccct ttattgagcg aaaacttttt tcagtcaatt ctggacatgc aacttcagct
     5101 tacattggtg cgcattatgg tgccaagaca attttggaag ctcttcaaaa tcctaatatc
     5161 aaatctcgga ttgaatctgt attagctgaa attcggagtc tcttgattgc caaatggaac
     5221 tttgataaaa aagaattgga gaattatcac aaagtcatta tagaacgatt cgaaaaccct
     5281 ttcatagtgg acgaggttag tcgcgtagct cgtactccaa tccgaaaatt aggctataat
     5341 gaacgattca tccggccgat acgtgaattg aaagaactca gtttgtcata taaaaaccta
     5401 cttaaaacag ttggctatgc ctttgactat cgcgatgtaa atgatgaaga aagtattcga
     5461 ttaggtgaat tgttggctaa acaatcagtc aaagatgttg ttatacaagt tacaggttta
     5521 gacgaccaag aattgattga gcaaattgta gagtatattt aatctttttc gaaaatctct
     5581 tcaaatcagg ttagcatcca tctgcaatct caaaacagta ttttgagctg acttcgtcag
     5641 ttctatctac aacctcaaag cagtgctttg agctgactcc gtcagtctta tctgcaacct
     5701 caaaacactg tttgagttat ctgcggtaat ctttctagct tgtctttgat ttttgttgtt
     5761 atttataagg taaaagaagc tggacaaaaa gtcttcaaaa tcggaaaaag gcagcctatc
     5821 gggtgttcaa aaatcttgat aggatgtcct ttattatgga aagccttatt ggattttctc
     5881 ctcagattga gtttttgatc agctttatga gataggtttt gctagagatg tagcccatca
     5941 tgttattttt atggacagtg ggaaaattgt tgaaaaaaat aatgcccatc aattctttag
     6001 tcgtccaaga gaagaacgaa ccaagcaatt ttggaacgaa ttctttcgaa tgtgatctat
     6061 atagtaaaat gaaacaagaa caggacaaat cgatcaggac agtcaaatcg atttctaaaa
     6121 atgttttaga agtagaggtg tactattcta gtttca
//
LOCUS       DAVHEW010000104         6148 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVHEW010000104 DAVHEW010000000
VERSION     DAVHEW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.104"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(44..319)
                     /locus_tag="VVL53_001865"
     CDS             complement(44..319)
                     /locus_tag="VVL53_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4183985.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(425..1264)
                     /locus_tag="VVL53_001866"
     CDS             complement(425..1264)
                     /locus_tag="VVL53_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU4183986.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1406..2254
                     /locus_tag="VVL53_001867"
     CDS             1406..2254
                     /locus_tag="VVL53_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU4183987.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2347..3264)
                     /locus_tag="VVL53_001868"
     CDS             complement(2347..3264)
                     /locus_tag="VVL53_001868"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU4183988.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3702..3907
                     /locus_tag="VVL53_001869"
                     /pseudo
     CDS             3702..3907
                     /locus_tag="VVL53_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4218..4511)
                     /gene="rpmA"
                     /locus_tag="VVL53_001870"
     CDS             complement(4218..4511)
                     /gene="rpmA"
                     /locus_tag="VVL53_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU4183989.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4528..4872)
                     /locus_tag="VVL53_001871"
     CDS             complement(4528..4872)
                     /locus_tag="VVL53_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU4183990.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4888..5202)
                     /gene="rplU"
                     /locus_tag="VVL53_001872"
     CDS             complement(4888..5202)
                     /gene="rplU"
                     /locus_tag="VVL53_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU4183991.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5238..5325)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5384..6088)
                     /locus_tag="VVL53_001873"
     CDS             complement(5384..6088)
                     /locus_tag="VVL53_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU4183992.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
BASE COUNT         1885 a         1266 c         1033 g         1964 t
ORIGIN      
        1 accaagctag aaagcttgat acaataggct ttttaaagac tgattattta acagcgtctt
       61 taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc atttctttac
      121 cagtttgtgg gttgcgacct ttacgttctg cgcgctcacg aacttcaaag ttaccaaaac
      181 cgatcaattg aactttttca ccagctgcaa gatagtcagc tactgctgca aatacagctt
      241 caactgctgc tgctgagtct ttcttagtca attctgtagc ttctgctact ttagcgatca
      301 aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta acaaatataa
      361 tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc attttattta
      421 ttttttagga gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg tgagtttgaa
      481 taatggaacc cgtttccaaa acagaaattg gcttttcaac ataagcttgt aagctttctt
      541 tcatctcttt tgcccaatca tcactaccag aatatgaaat tccaatctct gctacagcac
      601 gttcagaaag cgatgttatc aactcatcta accatttttt aaatgtttta gttccacgac
      661 ctttaaccat tggctgcaat tcatggtctt tcatttgcat gacagcacgg atattgagaa
      721 gagagctcaa caagccagtt acacggctaa ttcgtccacc tttgacaaga ttttccaaag
      781 ttgaaacacc aatataaagc tctgtatggt ttttaacctc ttctacatga gataaaattg
      841 cctccatatc tttaccttct tgagctaact tcgcagcctc aacaacttgg aatttcaggg
      901 cttggtcagt gaaggaacta tcaacaacag tcacatctgc agtagatagg ctagcacctt
      961 ggcgtgctgc ttctaccgta cccgaaagag catgggacat atgaatagca agaatctggc
     1021 caccatcttt gcataggtct tcaaaaatct cagcaaagac acctacaggt ggctgacttg
     1081 ttttcggaag attcttactt tcttgcatca actgaagaaa tttaccttct tctttcaaat
     1141 ccgcatcaga ataaacaaca ttatcaatca ttacagataa tggaacaatt gtaatatcta
     1201 attgctttac tagttcaggt tcaatagtaa cagatgaatc ggttacaatc ttaatttttg
     1261 tcatagtatc aatctttcta ttttaggatt cagattggtt tccttacttc taattatatc
     1321 aaaaaaaaga ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat ttgatataat
     1381 caacttataa gaaaagaggt gtcctatgat taaaaaaatt taccccattt ttaccatttt
     1441 actaggtgct gctatttatg cttttggact gacttatttt gtagttcccc atcatctctt
     1501 tgaaggaggg gcgacaggca ttaccctcat caccttttat ctttttaaaa tccctgtttc
     1561 cctcatgaac ctgctgatta atattcccct tttcatccta gcttggaaga tttttggagc
     1621 caaatccctc tattctagtt tactaggaac cttagctttg tccggctggt tagctttttt
     1681 tgagcatatt ccccttcata ttgatcttca aggtgattta ctaatcacag cccttatagc
     1741 gggaatccta ttgggaattg gccttggaat tatttttaat gctggaggta caactggcgg
     1801 aactgatatt ctagctcgta ttctcaacaa atacactcat atatccatag gaaaactgct
     1861 ctttatctta gatttttgta ttctcatgtt gattctccta atcttcaagg atttgagatt
     1921 ggtttcctac acgcttttgt ttgattttat tgtttctcgt gttattgatt tgattggtga
     1981 aggaggatat gccggcaaag gctttatgat tatcacaaaa cgtcctgacc aacttgctaa
     2041 ggcgattaat gatgacctcg gaagaggtgt tacttttatt tctggtcaag gctactatag
     2101 taaagaaaat ttgaaaatca tctactgtat tgtcggaaga aatgaaattg tgaaaacgaa
     2161 ggaaatgatt catcgaatcg atcctcaagc ctttataact attacagaag cccatgaaat
     2221 cctaggagaa ggcttcacct ttgaaaaaga ataaaaagag gtaatgtcgt gacctcaaaa
     2281 gttagactaa atcatctatc ttttgggtta cagacaacct cttttttatt ttatttactc
     2341 aagctcttaa gaccaattcc gagttacttc ttcatcagcc tttaactgat ccactaattg
     2401 gtcaactgag tcaaatttgg tcatatctcg aatgcgatca agccaataaa ccatgacggt
     2461 ttccccataa atatcttgat taaaatcaaa aatattgact tcaaaacgtg cttcttctcc
     2521 atcaaaggtc acatttttcc cgacactagc catagcacga tacttctgtc tttgaatctc
     2581 aacatcaaca acataaacgc catctgctgg catataagta cggtctaaaa gcactaaatt
     2641 cgctgtcgga taaccaattg tacgaccacg agcattacca tgaaccacca tacctcttga
     2701 tggaagcggt gcccccaaaa gttttcctgc ttctttcaca tttccatcta aaatagcttg
     2761 acggatacga gttgaactaa tctttccttt ctcatcttct acaggtggaa caatgataac
     2821 ttctccatca aagtaatcct ttaaatcttc tgctgttttt ttgtcagaac caaatgtata
     2881 atcaaaacct gcaacaataa ttttggcatt catagccttg atataagttg caaagaattc
     2941 ttgtgcagtg agactagcga attgactact aaaatcaagg agatataatt cttctacacc
     3001 ttcgcgcttt aattttcttt cacgttcagc agggttcaaa atatgcaaaa acaaatctgg
     3061 atgataaggc tctaaagcga tctttggaga ttcattaaag gtcataacga cgataggcaa
     3121 caaatccttt ctcgcagcct tgttggcaac acgaaataat tcttgatgcc ccttatgtat
     3181 gccatcaaaa tagccgagaa caacgactga atcagatggt gtgccaatat ctttttggtt
     3241 ttttatagga atagtaataa tcataaaata attatatcat agcgatagct atttctggaa
     3301 cagaaaatct gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa aaagcacttt
     3361 attctatcgt tgcttaacta tgaactttgc aatattcttc tcaaaaactt gtaggacatc
     3421 ttcaaaattt tgcaaggagt gattagactt gttcggtaac cataaagtgt catactatgc
     3481 ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta attggtgccg
     3541 aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa aatgagacaa
     3601 cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag tcactttccc
     3661 taatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct ttccaacaac
     3721 tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc ttggattctc
     3781 agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc aagccattca
     3841 gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt atagtcaatt
     3901 gaaataaaat ctgaagaaat cgagtaggaa actcatatca atgtttaaca gtgttctatt
     3961 ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc aggctgtact
     4021 tgagtacggc aaggtgaaat tgacatggtt tgaatttgat tttcgaagag tatcaatcca
     4081 ctatacatat taaaaagtat gggtaccaaa acaaaaaaac aggctctccg aaaactcgga
     4141 aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt tcgggataaa
     4201 aggttcaatg gaccttttta tttagcgatt gggtaaacag aaacttgttt tttatcgcgt
     4261 cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc atctccacca
     4321 cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag gattgatcca
     4381 cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg tgaatcacgt
     4441 ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt gttaagagtc
     4501 atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg tttggacgaa
     4561 ctcagaatag ttctccgata agtttgccat acctaagaaa aatgattcaa agaataactg
     4621 ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca gatagccacc
     4681 ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa tagaattgat
     4741 aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt attcgccact
     4801 ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc tctcaaagac
     4861 tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga caacttttgt
     4921 atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt acttgtaagt
     4981 aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag ctccagcaac
     5041 aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt taaaagtaac
     5101 ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa cttcaacttt
     5161 aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc tcctatgatt
     5221 tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac gaaccactta
     5281 aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca tagtcaactc
     5341 ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat gaagcttttt
     5401 cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg ctcatcataa
     5461 taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct gaaaagacaa
     5521 cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc aaaaaaggag
     5581 tgtgcttggt tcggaaataa tctccaaaag ccagcatggt ccgtttgata ttccctgtca
     5641 taaaagcgtt attataggca atacccgaca cttctccaaa agcagttgtc accagtccca
     5701 tacagaaggc caagggcggc actagataga tattatccac agtttgcggc acaaaagcaa
     5761 taatgattga taagattgcc aagggaatca aggacagaat aggttttttc acaattctca
     5821 atttttcctt ataaatcgtt aataaaaaga ctcccatcat aaacgctagc aaggtgagaa
     5881 ccttgtccct aacatccgaa acattatttt taattaattc tactgaaaga aagacaacat
     5941 ttccagtttg tccagctaca agggtattcc cgcgaacaat aaaagtgtaa gcatccacat
     6001 atccagcaca aaacgtcaaa aaaagtgcta accttttaga ctgacgtgat atttttctta
     6061 taggtaataa cctcatttta cctcccattg tattttctct tagaaatatt gtaccatttt
     6121 ctttctaaaa aatcgtaggc taccattt
//
LOCUS       DAVHEW010000105         5995 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVHEW010000105 DAVHEW010000000
VERSION     DAVHEW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.105"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..210)
                     /locus_tag="VVL53_001874"
     CDS             complement(<1..210)
                     /locus_tag="VVL53_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183993.1"
                     /translation="MNTIERTRRLIKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDET"
     gene            complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="VVL53_001875"
     CDS             complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="VVL53_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU4183994.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(1709..2308)
                     /locus_tag="VVL53_001876"
     CDS             complement(1709..2308)
                     /locus_tag="VVL53_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4183995.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(2309..3406)
                     /locus_tag="VVL53_001877"
     CDS             complement(2309..3406)
                     /locus_tag="VVL53_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4183996.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILSVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            complement(3406..4143)
                     /locus_tag="VVL53_001878"
     CDS             complement(3406..4143)
                     /locus_tag="VVL53_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4183997.1"
                     /translation="MKRWLALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(4145..5029)
                     /locus_tag="VVL53_001879"
     CDS             complement(4145..5029)
                     /locus_tag="VVL53_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4183998.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            complement(5016..5210)
                     /locus_tag="VVL53_001880"
     CDS             complement(5016..5210)
                     /locus_tag="VVL53_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4183999.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(5207..5338)
                     /locus_tag="VVL53_001881"
     CDS             complement(5207..5338)
                     /locus_tag="VVL53_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEU4184000.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKELEE"
BASE COUNT         1913 a         1348 c          968 g         1766 t
ORIGIN      
        1 agtttcatct acaacctcaa aaacatgttt tgagctgact tcgttagttt catctacaac
       61 ctcaaaaaca tgttttgagc tgacttcgtc agttctatct gcaacctcaa agcagtgctt
      121 tgagctgact tcgtcagttc tatctacaac ctcaaaacag tgtgttgcgc agcctttaat
      181 cagccgccta gtccgctcta tggtattcat taagtcaaca tctcttgttt aagagcacca
      241 aatcaggaaa tcttctcgat tccctgattt tttctattta cgttttcgtg ttgagctacg
      301 ttctgtcaaa ccatgaggta agagaacttc acgttcttcc aactcttcct tatgcataat
      361 cttggtcaac atacgcatac taatggcacc aaggtcataa agaggttggg caatcgttgt
      421 caagtttgga cgggtaaagc gtgagatttg tgaatcatca ctagtaataa tttcaaaatc
      481 ttctggcaca gaaacaccct tatcagccaa accgttcaag actcctgctg ccaactcatc
      541 acctgtcaca actgctgcag ttgcatttga tgaaatcaaa cgctctgcta aggcgtaacc
      601 atcatcatag ctatatttag attcaaatac caaaccctca ctataagtga ttcctgcttt
      661 tttcaaggtt tccttgtagc caactaaacg aaccttacca ttgatgtcat ccactagcgg
      721 accgctaacg aaagcaatac gctcattttc tttagcaagg taactcactg catcaattgt
      781 tgcttgctta tagtcaatat tgacacttgg caactggtgc tcaacatcga cagttcctgc
      841 gagaacaatc ggagtacgtg aacgcgaaaa ttctgagcga attttatctg tcaagtgata
      901 ccccatatag ataatgccat ctacctgctt tgaaaagagg gtattgacaa cagaaacttc
      961 tttctcgtta tcttcatcgc tattagctag gacaatattg tacttgtaca tttctgcaat
     1021 atcatcaatc cccttagcca aactcgaaaa ataaccattg gtaatatttg gaatcacgac
     1081 accgacagtg gttgtctttt tacttgcaag accacgcgca actgcatttg gacgataatc
     1141 caaacgatca attacctcta gcactttttt acgggtattc tcttttacat ttttattgcc
     1201 attgaccaca cggctgaccg tcgccatgga aacacctgct tcacgagcga catcataaat
     1261 ggttactgta tcatctgcat tcattccttt tcctgtcctt tctatctcac acattctttt
     1321 acaagtagag gtactgattg aagctctata tctacttaca aaagtgaaga tgtgaaaatt
     1381 tcgttttcat atttctactt attccattct atcactaatt gtaaacactt tcaagtgttt
     1441 tttgaagatt gattgaaaaa atttcataga aaacctaggt ttagctcctt gctaccacct
     1501 tagactaaac aaaaaggagg aaactaagcc ctcctaaagt tatagtaaaa tgaaataaga
     1561 acaggataaa tcgatcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt
     1621 gtactattct agtttcaatc tactataggt attgttccat tcactaccgt caattttagc
     1681 acatagtctt catgaaaata ttatatcatc ataaccaacc agattctttc gcgatattag
     1741 ctgcctctgt tcgattacct gcatctagtt tcgaaagaat attggtgaca tagtttcgga
     1801 ctgttccgtt ggatagataa agtttgtctg caatttcttg gttagagaag ccctgagcaa
     1861 ttccctttaa aactgcgatt tcttgctccg ttaatggatt gggatgcatc atcaccactt
     1921 ccatcaattc aggcgaatac tccttgcgtc cttcgaggac ggtgtgcaag gtttgcatga
     1981 ggtctgcaat gtttctttct tttaatacat aagcatctac tccagccttg accgcacgtt
     2041 caaaataccc aggacgcttg aaggtcgtca ccacaaccac ctttgtttca agcttttctg
     2101 ctcgtatcca ctccaagact tcaagacctg tcttaacagg catttctacg tcaaggatgg
     2161 cgatatctac agactccttt tctaatagtt ggattgcttc ttgcccattc ttggcttgaa
     2221 agacagactc tacatccggt tgaagcatga gcaactggca catggcatct cgcaacatac
     2281 tttgatcttc tgcgactaat actttcatct actttctctc cttataaagt agtcgaacct
     2341 gcacttcagt tggatgtttc tgactgatta cacttacttc tcctgaaaat ggaaaaacac
     2401 gatttcggac tgtatggagc tcatccccgc ttatagaggc aaagccacag ccatcatctc
     2461 tcactgttag aatgagttct ttctctgtcc gttctaattt caagtagact ttagacgctt
     2521 tagcatgttt gatgatattg gtcactaatt caagcaaaat catggaagcc gttgactcca
     2581 attcctgagt taagctagac ttgtccaagt gattctcaac ttgaacctca attccagcaa
     2641 tttctaacat ctttttcaca gtctctagtt cggatgtcaa agttctagac ttaagatttt
     2701 ccacaatggt tcgcacttca ttcatggaat ccttgctgat ctggtgaatt tcttttaatt
     2761 ccttttccac ctgtggataa gcctccatct gaaataactg caaggctaaa tctgtcttga
     2821 cactcagcat agcaaaggta tgtcccagac tatcatgcaa atcctgaccg atacgactac
     2881 gttcattttc agcaagcaat agatttatct gagcattttg cttgacctga gcttctttca
     2941 aatcctcgac aatacgaatc cgaaccaatc caaaagtcat taaatcgaca aaagtaagaa
     3001 ttacaagtag atagaataga aactcaactt cgattctctg aaaaatcaac agttgcccca
     3061 caacaaggac ttgagcaaga agaaaagtcc agacatgtaa agactttaaa ctacgtacgc
     3121 tgaaatgata acttaagaga ttggatagga aaaagaaaaa ccagatataa ttaacagcaa
     3181 caaaggcagt attcccaact acataagtca gcatgaggcc ccaatatagc caagataggc
     3241 gctggctctt agttgttaaa acacccaaat atgccactac aaatagaata tcaatcaata
     3301 aatgccaggc agaaagccac ccagtcacta cagacaggat ggggaaaatc ataaaaatta
     3361 aactgatcca aaacatataa tgtattcttt tcagtctttc aagcattaag cattctcctt
     3421 atgaccttga aggtaaatgg tcaaaccaaa caaaactact gaaaaaacaa gtaaataaac
     3481 tgtggctgat agattgatgc caccctcatt taagaaggtc ttgagcaact ccatcaactg
     3541 ataggtcggg agacacttac ctactacttg catccagtct ggaaataaag agataggcat
     3601 ccagagtcca cctaaaacag ccaaccctag ataaagaaga ttgcccacga cagacatcaa
     3661 ctgactagtt ggtaagagag tcaaggtcaa accaagcgct acgaaggcaa tacttcctac
     3721 tatcagcaaa agtgcagccc caatccaatt tccaagagac atgtccacac ctcttacaaa
     3781 atgcccaact gagaaaacca ccaagattga aaccaaataa tcaaccagca tacttgttat
     3841 ctttgataga taatattcta ccatatttac agggctatga cgcaatgttt tctgccagtt
     3901 gttgatcttg tcggtatgta aaacaactgg gaatgagaag atagctgttg acatcatgga
     3961 aaatgcagtc atggagataa gataatcacg cataaaattc gcgagttcac ctggtgtgtc
     4021 ctgatagata ccagaaaaaa ataaatagaa agccgtcggc atccctactg acaatagata
     4081 atagatcaat tgtcgtttgg tcaataaaaa ttctatctta ctaagtgcta gccatcgttt
     4141 catcttagtt atctcccttc tgcgtttctt caaagattgt atccaacaaa ctacgattat
     4201 taacttcaat ttcttgtatg ccacatcctg cttgaactaa cagttcccaa aaagcatctg
     4261 cttcgcgtgt gactacttgt agagcatcct gtttttgtga ccagttttca accaagttag
     4321 actgctcaat gacttccttg tatgccagag gaaggataaa atgcttttca attccctcac
     4381 tacgcatagc tagaggcgtc gtatcacgaa tcaactctcc cttatttaaa accaaaatcc
     4441 ggtcagccgt atgctctacc tcttcaatat aatgagacga atagagaatc gtgactcctt
     4501 gcgcttttag gtcccgaacg atttcccaaa agcgttgacg agttgaagta tccatggcag
     4561 cagttggttc atctaaaaag acaagctttg gtcgcccaat caaggtcaag acaaaagaga
     4621 agagacgctt ttgcccgcct gacaattttt ctgcgaattg ctctttttgt tgctggtcaa
     4681 actgcaatag ttgatcgatt tcctgatcgc tcaaggaatt tggatagata cgttgaaaga
     4741 aagcaatcaa ctctttgacc tttaatttct gaacgatgac attttcttga ggcagataac
     4801 ctctaatata gtctaactga gaactcgtca ctgacaagcc ttggatggat acttgaccgc
     4861 ttgtgaccag tttatctcca agcagacagt ccaagagtgt ggtcttccca gcaccattgg
     4921 gcccaatcaa ggcgacgcat tcaccttcag ctacctcaaa ggaaataccc ttcaaaatag
     4981 ccttgccctt gatgttttta tttaggcttt ctaccttaat catattcatg atattctcct
     5041 ttcaaccact ccattctcat aaggaaaacg acgaaaatca taaatccaaa ccccaaagca
     5101 ccacgaatga attggcgaag caaggtttgg tcaaaccaac ctgtaaacat ttccactaac
     5161 cataccaaga gtgacaggcc gataaagaaa tagatgatcc ctctcttcat tcctcaagct
     5221 cctttttcac atctccgact aatttcaaac cttctctaac aagccaagac atcattccaa
     5281 agccagcaaa gagctcccaa ggaaaatgat agaaactctc atccaatccc gaaaacatga
     5341 gttaggtcat aactcctgct actactaaac tcactgcgat aatcatttta tttctcatct
     5401 cttcttcctc catttcatac tacaattata gtcttttgaa atcagaggag acagaagctt
     5461 ctgtcactag aaaatatgac aaatgtcata aaaaattctg ttcaaaacaa gcaagataca
     5521 ctatacaata aaacacaatt agaaaaatct aaggcaactt cctcaaaaga gatatcaaac
     5581 ccaattcaca ccataatgta aactaatact tatttaaaat caaaaagagt agaaattttt
     5641 atcagacaaa cacatatata gtgtattgaa tctataacag taggccttaa atactaaaat
     5701 atttctataa attaatttaa ctttcctgat agagctgttc atatcttatt tcaattctct
     5761 aaattatacg ttgaacaaaa cccttctatt tctttcttaa agatttataa gagttataaa
     5821 atctgttaaa tttcaatgtg tatacctaaa ctacggtatt tattgaaaag actggagaca
     5881 aaaagtatac gctgccaaaa tgaattactg aaaatcaaaa aagagagaac caaactgatt
     5941 ccctcttaat gtatataata tctagtttta aaaatacaca ctcacatatc tctgt
//
LOCUS       DAVHEW010000106         5882 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVHEW010000106 DAVHEW010000000
VERSION     DAVHEW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.106"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            81..560
                     /locus_tag="VVL53_001882"
     CDS             81..560
                     /locus_tag="VVL53_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4184001.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            654..725
                     /locus_tag="VVL53_001883"
     tRNA            654..725
                     /locus_tag="VVL53_001883"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:687..689,aa:Glu,seq:ttc)
     gene            979..2524
                     /locus_tag="VVL53_001884"
     rRNA            979..2524
                     /locus_tag="VVL53_001884"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2576..2648
                     /locus_tag="VVL53_001885"
     tRNA            2576..2648
                     /locus_tag="VVL53_001885"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2609..2611,aa:Ala,seq:tgc)
     gene            2773..5674
                     /locus_tag="VVL53_001886"
     rRNA            2773..5674
                     /locus_tag="VVL53_001886"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5751..5866
                     /gene="rrf"
                     /locus_tag="VVL53_001887"
     rRNA            5751..5866
                     /gene="rrf"
                     /locus_tag="VVL53_001887"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1735 a         1140 c         1680 g         1327 t
ORIGIN      
        1 gagctaaagg gaacagaatg gagaaaatgg aacaaatgtg ttttctaatc tgttagactg
       61 tatctagaaa ggggaaaatt atgattaaag aattgtatga agaagtccaa gggactgtgt
      121 ataagtgtag aaatgaatat taccttcatt tatgggaatt gtcggattgg gagcaagaag
      181 gcatgctctg cttacatgaa ttgattagta gagaagaagg actggtagac gatattccac
      241 gtttaaggaa atatttcaag accaagtttc gaaatcgaat tttagactat atccgtaaac
      301 aggaaagtca gaagcgtaga tacgataaag aaccctatga agaagtgggt gagatcagtc
      361 atcgtataag tgaggggggt ctctggctag atgattatta tctctttcat gaaacactaa
      421 gagattatag aaacaaacaa agtaaagaga aacaagaaga actagaacgc gtcttaagca
      481 atgaacgatt tcgagggcgt caaagagtat taagagactt acgcattgtg tttaaggagt
      541 ttactatccg tacccactag taagtcatgc aaaaaaaatg aaaaaaatta gaaaaagtag
      601 ttgacaaagt ttgaaaaggc tgtataatag taagagttga aaataacaac tcaggtccgt
      661 tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg
      721 gactatggta tgttgcgtca ggaccacttg atgaaaaaaa gtttaaaaaa acttaaaaat
      781 cttcaaaaaa gtgttgacaa gcgaaagcag ttgtgatata ctaatatagt tgtcgcttga
      841 gagaagcaag tgacaaagac ctttgaaaac tgaacaagac gaaccaatgt gcagggcgct
      901 acaacgtaag ttgtagtact gaacaatgaa aaaaacaata aatctgtcag tgacagaaat
      961 gagtaagaac tcaaactttt taatgagagt ttgatcctgg ctcaggacga acgctggcgg
     1021 cgtgcctaat acatgcaagt agaacgctga aggaggagct tgcttctctg gatgagttgc
     1081 gaacgggtga gtaacgcgta ggtaacctgc ctggtagcgg gggataacta ttggaaacga
     1141 tagctaatac cgcataagag tagatgttgc atgacatttg cttaaaaggt gcacttgcat
     1201 cactaccaga tggacctgcg ttgtattagc tagttggtgg ggtaacggct caccaaggcg
     1261 acgatacata gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga
     1321 ctcctacggg aggcagcagt agggaatctt cggcaatgga cggaagtctg accgagcaac
     1381 gccgcgtgag tgaagaaggt tttcggatcg taaagctctg ttgtaagaga agaacgagtg
     1441 tgagagtgga aagttcacac tgtgacggta tcttaccaga aagggacggc taactacgtg
     1501 ccagcagccg cggtaatacg taggtcccga gcgttgtccg gatttattgg gcgtaaagcg
     1561 agcgcaggcg gttagataag tctgaagtta aaggctgtgg cttaaccata gtaggctttg
     1621 gaaactgttt aacttgagtg caagagggga gagtggaatt ccatgtgtag cggtgaaatg
     1681 cgtagatata tggaggaaca ccggtggcga aagcggctct ctggcttgta actgacgctg
     1741 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gctgtaaacg
     1801 atgagtgcta ggtgttagac cctttccggg gtttagtgcc gtagctaacg cattaagcac
     1861 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca
     1921 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat
     1981 ccctctgacc gctctagaga tagagctttc cttcgggaca gaggtgacag gtggtgcatg
     2041 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccccta
     2101 ttgttagttg ccatcattta gttgggcact ctagcgagac tgccggtaat aaaccggagg
     2161 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca
     2221 atggctggta caacgagtcg caagccggtg acggcaagct aatctcttaa agccagtctc
     2281 agttcggatt gtaggctgca actcgcctac atgaagtcgg aatcgctagt aatcgcggat
     2341 cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga
     2401 gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc cagccgccta aggtgggata
     2461 gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct ggatcacctc
     2521 ctttctaagg ataaggaact gcgcattggt cttgtttagt cttgagaggt cttgtggggc
     2581 cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt cgatcccgct
     2641 aggctccatt ggtgagagat caccaagtaa tgcacattga aaattgaata tctatatcaa
     2701 atagtaacaa gaaaataaac cgaaaacgct gtagtattaa taagagttta tgactgaaag
     2761 gtcagaaaat aaggttaagt taataagggc gcacggtgga tgccttggca ctaggagccg
     2821 acgaaggacg tgacaaacga cgatatgcct tgggtagctg taagtaagcg atgatccagg
     2881 gatttccgaa tgggggaacc caacaggtaa tacctgttac ccacatctgt taaggatgtg
     2941 aggaggaaga cgcagtgaac tgaaacatct aagtagctgc aggaagagaa agcaaaagcg
     3001 attgccttag tagcggcgag cgaaacggca gaagggcaaa ccgaagagtt tactcttcgg
     3061 ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga atgatttggg aagatcagcc
     3121 aaagagagta atagcctcgt atttaaaata gtctttgtac ttagcagtat cctgagtacg
     3181 gcgggacacg tgaaatcccg tcggaatctg ggaggaccat ctcccaaccc taaatactcc
     3241 ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc cgggagggga
     3301 gtgaaataga acctgaaacc gtgtgcctac aacaagttcg agcccgttaa tgggtgagag
     3361 cgtgcctttt gtagaatgaa ccggcgagtt acgttatgat gcgaggttaa gttgaagaga
     3421 cggagccgta gggaaaccga gtctgaatag ggcgccttag tatcatgacg tagacccgaa
     3481 accatgtgac ctacccatga gcaggttgaa ggtgcggtaa gacgcactgg aggaccgaac
     3541 cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc ggagaaattc caaacgaact
     3601 tggagatagc tggttctctc cgaaatagct ttagggctag cgtcgacatt agagattctt
     3661 ggaggtagag cactgtttgg gtgaggggtc catcccggat taccaatctc agataaactc
     3721 cgaatgccaa tgaattatgg tcggcagtca gactgcgagt gctaagatcc gtagtcgaaa
     3781 gggaaacagc ccagaccacc agctaaggtc ccaaaataat tgttaagtgg aaaaggatgt
     3841 ggggttgcac agacaactag gatgttagct tagaagcagc tattcattca aagagtgcgt
     3901 aatagctcac tagtcgagtg accctgcgcc gaaaatgtac cggggctaaa acaatttacc
     3961 gaagctgtgg atacctttat aggtatggta ggagagcgtt ctatgtgtga tgaaggtata
     4021 ccgtgaggag tgctggaacg catagaagtg agaatgccgg tatgagtagc gaaagacagg
     4081 tgagaatcct gtccaccgta agactaaggt ttccagggga aggctcgtcc gccctgggtt
     4141 agtcgggacc taaggagaga ccgaaaggtg tatccgatgg acaacaggtt gatattcctg
     4201 tactagagta tgtagtgatg gagggacgca gtaggctaac taaagcagac gattggaaga
     4261 gtctgtctaa gcagtgaggt gtgaattgag tcaaatgctt aattctataa cattgagctg
     4321 tgatggggag cgaagtttag tagcgaagtt agtgacgtca cactgccaag aaaagcttct
     4381 agcgtttaaa catactctac ccgtaccgca aaccgacaca ggtagtcgag gcgagtagcc
     4441 tcaggtgagc gagagaactc tcgttaagga actcggcaaa atgaccccgt aacttcggga
     4501 gaaggggtgc tgacttaaag tcagccgcag tgaataggcc caagcaactg tttatcaaaa
     4561 acacagctct ctgctaaatc gtaagatgat gtataggggg tgacgcctgc ccggtgctgg
     4621 aaggttaaga ggagtgctta gcgtaagcga aggtatgaat tgaagcccca gtaaacggcg
     4681 gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacccgca
     4741 cgaaaggcgt aatgatttgg gcactgtctc aacgagagac tcggtgaaat tttagtacct
     4801 gtgaagatgc aggttacccg cgacaggacg gaaagacccc atggagcttt actgcagttt
     4861 gatattgagt gtctgtacca catgtacagg ataggtagga gtctaagaga tcgggacgcc
     4921 agtttcgaag gagacgctgt tgggatacta cccttgtgtt atggccactc taacccagat
     4981 aggtgatccc tatcggagac agtgtctgac gggcagtttg actggggcgg tcgcctccta
     5041 aaaggtaacg gaggcgccca aaggttccct cagaatggtt ggaaatcatt cgcagagtgt
     5101 aaaggtataa gggagcttga ctgcgagagc tacaactcga gcagggacga aagtcgggct
     5161 tagtgatccg gtggttccgt atggaagggc catcgctcaa cggataaaag ctaccctggg
     5221 gataacaggc ttatctcccc caagagttca catcgacggg gaggtttggc acctcgatgt
     5281 cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa
     5341 gcggcacgcg agctgggttc agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc
     5401 gtaggaaatt tgagaggatc tgctcctagt acgagaggac cagagtggac ttaccgctgg
     5461 tgtaccagtt gtcttgccaa aggcatcgct gggtagctat gtagggaagg gataaacgct
     5521 gaaagcatct aagtgtgaaa cccacctcaa gatgagattt cccatgatta tatatcagta
     5581 agagccctga gagatgatca ggtagatagg ttagaagtgg aagtgtggcg acacatgtag
     5641 cggactaata ctaatagctc gaggacttat ccaaagtaac tgagaatatg aaagcgaacg
     5701 gttttcttaa attgaataga tattcaattt tgagtaggta ttactcagag ttaagtgacg
     5761 atagcctagg agatacacct gtacccatgc cgaacacaga agttaagccc tagaacgccg
     5821 gaagtagttg ggggttgccc cctgtgagat agggaagtcg cttagctcta gggagtttag
     5881 ct
//
LOCUS       DAVHEW010000107         5765 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVHEW010000107 DAVHEW010000000
VERSION     DAVHEW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.107"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            111..1811
                     /locus_tag="VVL53_001888"
     CDS             111..1811
                     /locus_tag="VVL53_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU4184002.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1804..2280
                     /gene="ilvN"
                     /locus_tag="VVL53_001889"
     CDS             1804..2280
                     /gene="ilvN"
                     /locus_tag="VVL53_001889"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU4184003.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2346..3368
                     /gene="ilvC"
                     /locus_tag="VVL53_001890"
     CDS             2346..3368
                     /gene="ilvC"
                     /locus_tag="VVL53_001890"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU4184004.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3475..3711
                     /locus_tag="VVL53_001891"
     CDS             3475..3711
                     /locus_tag="VVL53_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184005.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3949..4305
                     /locus_tag="VVL53_001892"
     CDS             3949..4305
                     /locus_tag="VVL53_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU4184006.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            4355..5605
                     /gene="ilvA"
                     /locus_tag="VVL53_001893"
     CDS             4355..5605
                     /gene="ilvA"
                     /locus_tag="VVL53_001893"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU4184007.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1786 a          977 c         1322 g         1680 t
ORIGIN      
        1 aataaacgtt aaaatagaaa attcagaaaa tttcttcttt tatcttgaaa aattttgaaa
       61 aaatggtatg atagtaacaa gttattttta agaggaaaga aaggggaata atggagaaaa
      121 tcagtttaga atctcctaag acggggtcgg acctagtttt ggaaacactt cgtgatttag
      181 gagttgatac catctttggt tatcctggtg gtgcggtttt gcctttttat gatgcgatat
      241 ataattttaa aggcattcgc cacattctag ggcgccatga gcaaggttgt ttgcatgaag
      301 ctgaaggtta tgccaaatca actggaaagt tgggtgttgc cgtcgtcact agtggaccag
      361 gagcaacaaa tgccattaca gggattgcgg atgccatgag cgatagcgtt ccccttttgg
      421 tctttacagg tcaggtggcg cgagcaggga ttgggaagga tgcctttcag gaggcagaca
      481 tcgtgggaat taccatgcca atcactaagt acaattacca agttcgtgag acagctgata
      541 ttccgcgtat cattacggaa gctgtccata tcgcaactac aggccgtcca gggccagttg
      601 taattgacct accaaaagac atatctgctt tagaaacaga cttcatttat tcaccagaag
      661 tgaatttacc aagttatcag ccgactcttg agccgaatga tatgcaaatc aagaaaatct
      721 tgaagcaatt gtccaaggct aaaaagccag tcttgttagc tggtggtgga attagttatg
      781 ctgaggctgc tacggaacta aatgaatttg cagaacgcta tcaaattcca gtggtaacca
      841 gtcttttggg acaaggaacg attgcaacga gtcacccact ctttcttgga atgggaggca
      901 tgcacgggtc attcgcagca aatattgcta tgacggaagc ggactttatg attagtattg
      961 gttctcgttt cgatgaccgt ttgacgggga atcctaagac tttcgctaag aatgctaagg
     1021 ttgcccacat tgatattgac ccagctgaga ttggcaagat tatcagtgca gacattcctg
     1081 tagttggaga tgctaagaag gccttgcaaa tgttgctagc agaaccaaca gttcacaaca
     1141 acactgaaaa gtggattgag aaagtcacta aagacaagaa tcgtgttcgt tcttatgata
     1201 agaaagagcg tgtggttcaa ccgcaagcag ttattgaacg aattggtgaa ttgacgaatg
     1261 gagatgccat tgtggtaaca gacgttggtc aacaccaaat gtggacagct cagtattatc
     1321 cctaccaaaa tgaacgtcag ttagtgactt caggtggttt gggaacaatg ggctttggaa
     1381 ttccagcagc aatcggtgct aaaattgcta acccagataa ggaagtagtc ttgtttgttg
     1441 gggatggtgg tttccaaatg accaaccagg agttggctat tttgaatatt tacaaggtgc
     1501 caatcaaggt ggttatgctg aacaatcatt cacttggaat ggttcgccag tggcaggaat
     1561 ccttctatga aggcagaaca tcagagtcgg tctttgatac ccttcctgat ttccaattga
     1621 tggcgcaggc ttatggtatt aaaaactata agtttgacaa tcctgagacc ttggctcaag
     1681 accttgaagt catcactgag gatgttccta tgctaattga ggtagatatt tctcgtaagg
     1741 aacaggtgtt accaatggta ccggctggta agagtaatca tgagatgttg ggggtgcagt
     1801 tccatgcgta gaatgttaac agcaaaacta caaaatcgtt caggagtcct caatcgcttt
     1861 acaggtgtcc tatctcgtcg tcaggttaat attgaaagca tctctgttgg agcaacagaa
     1921 gatccgaatg tatcgcgtat cactattatt attgatgttg cttctcatga tgaagtggag
     1981 caaatcatca aacagctcaa tcgtcagatt gatgtgattc gcattcgaga tattacagac
     2041 aagcctcatt tggagcgcga ggtgattttg gttaagatgt cagcgccagc tgagaagaga
     2101 gctgagattt tagcgattat tcaacctttc cgtgcaacag tagtagacgt agcgccaagc
     2161 tcgattacca ttcagatgac gggaaatgca gaaaagagcg aagccctatt gcgagtcatt
     2221 cgcccatacg gtattcgcaa tattgctcga acgggtgcaa ctggatttac ccgcgattaa
     2281 aaatccaact taaatttatt aaaccagcct aaaaggcaat aaataataga aaagagagaa
     2341 aagctatgac agttcaaatg gaatatgaaa aagatgttaa agtagcagca cttgacggta
     2401 aaaaaatcgc cgttatcggt tatggttcac aagggcatgc gcatgctcaa aacttgcgtg
     2461 attcaggtcg tgacgttatt atcggtgtac gtccaggtaa atcttttgat aaagcaaaag
     2521 aagatggatt tgatacttac acagtagcag aagctactaa gttggctgat gttatcatga
     2581 tcttggcgcc agacgaaatt caacaagaat tgtacgaagc agaaatcgct ccaaacttgg
     2641 aagctggaaa cgcagttgga tttgcccatg gtttcaacat ccactttgaa tttatcaaag
     2701 ttcctgcgga tgtagatgtc ttcatgtgtg ctcctaaagg accaggacac ttggtacgtc
     2761 gtacttacga agaaggattt ggtgttccag ctctttatgc agtataccaa gatgcaacag
     2821 gaaatgctaa aaacattgct atggactggt gtaaaggtgt tggagcggct cgtgtaggtc
     2881 ttcttgaaac aacttacaaa gaagaaactg aagaagattt gtttggtgaa caagctgtac
     2941 tttgtggtgg tttgactgcc cttatcgaag caggtttcga agtcttgaca gaagcaggtt
     3001 acgctccaga attggcttac tttgaagttc ttcacgaaat gaaattgatc gttgacttga
     3061 tctacgaagg tggattcaag aaaatgcgtc aatctatttc aaacactgct gaatacggtg
     3121 actatgtatc aggtccacgt gtaatcactg aacaagttaa agaaaatatg aaggctgtct
     3181 tggcagacat ccaaaatggt aaatttgcaa atgactttgt aaatgactat aaagctggac
     3241 gtccaaaatt gactgcttac cgtgaacaag cagctaacct tgaaattgaa aaagttggtg
     3301 cagaattgcg taaagcaatg ccattcgttg gtaaaaacga cgatgatgca ttcaaaatct
     3361 ataactaatt agaaatatat agcgctggag atgattttat gaaaaagatt atgagaaaaa
     3421 ttgcatcgtt attattggtt ctagttgtat aatgtaatta caccgtcggt aatagtgcta
     3481 gcagaccaaa ataaagcaga ttggtcgtat gatgaaaatg ctgtaattaa catttatgat
     3541 gatgctaatt ttgaagatgg taggttgcat atgaactttg aacaattctt caaattggca
     3601 caaatagcta gagaagaagg tcttgaaatt cattctccgt ttgagagagc tggtgcgact
     3661 aaatctgctc gttatatagc gaaatggatt ttgagaaata aaaaacatta acaaatatag
     3721 ttggtaaatc attaggacct aaatcagctg ttagattcgg agaagcttta tcctatattg
     3781 aaggtcctct tcgcagaata aatgagacga tagatggcgg tttatatcaa atagagcaaa
     3841 ttattgcatc tggattgaaa gaatcgggtt taaatgactg gactgcgaaa actttagctt
     3901 cagctattcg tgggatatta gatgtactta tttaggggtt gaaatcatat gaatattacc
     3961 aatttgtttt ctatcaagac aggatgtgat gaaactgata ggcaactgca aaaactattt
     4021 tttcagttgg atttacaatt gggagaattg acagatcaac taagaaaatt agattctaat
     4081 tttgttcctc gtagtcaatt tgtagacacg ttggatttga atgatgtaga atataaagaa
     4141 attttaaact attttatctt ccatcgtaat gatagtgaag aaagtttggt agaatggtta
     4201 tatgattgga tttccacaaa tcgttatgaa cttcctaaag agttttcgat tcgtatggct
     4261 cataaatacc atgaaagtgt tactgaagtt ttcggagatg aataactaaa aaacagtcat
     4321 tagtgactgt tttttataga aaaagaggtt ttatatgtta agttcaaaag atataatcaa
     4381 ggctcacaag gtcttgaacg gtgtggttgt gaatactcca ctggattacg atcattattt
     4441 atcggagaag tatggtgcta agatttattt gaaaaaagaa aatgcccagc gtgttcgctc
     4501 ctttaaaatt cgtggtgcct attatgccat ttcccagctc agcaaggaag aacgtgaacg
     4561 tggggtagtc tgcgcttctg cgggaaatca tgcgcaggga gtagcctata cttgtaatga
     4621 aatgaaaatt cctgctacta tctttatgcc cattactacg ccacaacaaa agattggtca
     4681 ggttcgcttt tttggtgggg attttgtaac tattaaacta gttggagata cctttgatgc
     4741 ctcagccaaa gcagctcaag aatttacagt ctctgaaaat cgtaccttta ttgatccttt
     4801 tgatgatgct catgttcaag caggtcaagg aacagttgct tatgagattt tagaagaagc
     4861 tcgaaaagaa tcgattgatt ttgatgctgt cttggttcct gttggtggtg gcggtctcat
     4921 tgccggggtt tctacctata tcaaggaaac aagtccagag attgaggtta tcggagtaga
     4981 ggcgaatgga gcgcgttcca tgaaagctgc ctttgaggct ggaggtccag taaaactcaa
     5041 ggaaattgat aaatttgctg atgggattgc tgtgcaaaag gtaggtcagt tgacctatga
     5101 agcaactcgt caacatatta aaactttggt aggtgtcgat gagggattga tttctgaaac
     5161 cttgattgac ctttactcta agcaagggat agtcgcagaa cctgctggag cggctagtat
     5221 cgcctcttta gaggttttag ctgaatatat taaggggaaa accatttgtt gtatcatttc
     5281 tggaggaaat aatgatatca accgtatgcc agaaatggaa gagcgtgcct tgatttatga
     5341 tggtatcaaa cattactttg tggtcaattt cccacaacgt ccaggagctt tgcgtgagtt
     5401 tgtaaatgat atcctggggc caaatgatga tatcacacgt tttgagtata tcaaacgagc
     5461 tagcaaggga acaggcccag tattaattgg gatcgcttta gcagataagc atgattatgc
     5521 aggtttgatt cgtagaatgg aaggttttga tccagcttat attaacttaa atggtaatga
     5581 aacgctttat aatatgcttg tctgaggact aataaaaaaa tatcatacct tcattttgat
     5641 ttcctatcta ttgacaagca tagtcacact gtctttaata ctcttcgaaa atctcttcaa
     5701 accacgttag ctctatctgc aacctcaaaa cagtgttttg agcaacttgc ggctagcttc
     5761 ctagt
//
LOCUS       DAVHEW010000108         5720 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVHEW010000108 DAVHEW010000000
VERSION     DAVHEW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.108"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            265..2589
                     /gene="pflB"
                     /locus_tag="VVL53_001894"
     CDS             265..2589
                     /gene="pflB"
                     /locus_tag="VVL53_001894"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU4184008.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(2727..4001)
                     /locus_tag="VVL53_001895"
     CDS             complement(2727..4001)
                     /locus_tag="VVL53_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4184009.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTSTSTVYRKLKQFHFQEDYTTLPEIL
                     SWDEFSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDM
                     SGSYIPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWK
                     FILKDSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNT
                     DQFWGLIQDTLPHLNRTFKTTLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRN
                     AFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(4266..>5720)
                     /gene="rlrA"
                     /locus_tag="VVL53_001896"
     CDS             complement(4266..>5720)
                     /gene="rlrA"
                     /locus_tag="VVL53_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU4184010.1"
                     /translation="IELDELSTLTSLQSKSLLSILQELQETFEEELTFNLDTQQVQLI
                     EHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQSFNEFTQKEYISIATGYRVRQKCGL
                     LLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFGIEIYDLNDGSMDWVTHMIVQSNSQL
                     SHELLEITPDEYVHFSILVALTWKRREFPLEFPESKEFEKLKNLFMYPILMEHCQTYL
                     EPHANMTFTQEELDYIFLVYCSANSSFSKDKWNQEKKTHTIQLILQHTRGKHLLSKFK
                     NILGNDISNSLSFLTALTFLTRTFLFGLQNLVPYYNYYEHYGIESDKPLYHISKAIVQ
                     EWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLPAIPIFIILNNQADVNLIKSIILRNF
                     TDKVASVTGYNILISPPPSEEHLTEPLIIITTKEYLPYVKKQYPKGKHHFLTIALDLH
                     VSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYLL"
BASE COUNT         1767 a         1030 c         1172 g         1751 t
ORIGIN      
        1 tctgcggcta gcttcctagt ttgcttttcg atttccattg agtgttactg cttattttct
       61 tttattatac ccttttttct gaaaaaaaga aaaaaggact ttattttttc aaaaatataa
      121 tacagtttga aataaaatat agactgtttt agaaaagaaa gtgtaaaaat agggaatttt
      181 cacttgttga aatcggttac tatatggtat acttgtctta tgaatgtaac agatgactgt
      241 tactagaaaa aagaggacat taatatggtt gttaagacag ttgttgaagc acaagatatt
      301 tttgacaaag cttgggaagg cttcaaaggc gtagattgga aagaaaaagc aagtgtatca
      361 cgatttgtac aagctaacta cacaccttat gatggagacg aaagcttcct tgcaggacca
      421 acagagcgtt cacttcacat caagaaaatt gtagaagaaa ctaaagcaca ctacgaagaa
      481 actcgtttcc caatggacac tcgtccaaca tctatcgctg atatccctgc tggatttatc
      541 gacaaagaaa atgaagttat cttcggtatc caaaacgatg aactcttcaa attgaacttc
      601 atgccaaaag gtggtatccg tatggctgaa actactttga aagaaaatgg atacgaacca
      661 gacccagctg ttcacgaaat cttcactaaa tatgtaacaa cagttaacga cggtattttc
      721 cgtgcctaca cttcaaatat tcgtcgcgct cgtcacgcac acactgtaac tggtcttcca
      781 gatgcatact cacgcggacg tatcatcggt gtttacgcac gtcttgctct ttacggtgca
      841 gactacttga tgcaagaaaa agtaaatgac tggaatgcaa tcaaagaaat cgatgaagaa
      901 acaatccgtc ttcgtgaaga agtaaacctt caataccaag cattgcaaca agttgttcgc
      961 ctgggtgacc tttacggggt tgatgttcgc aaaccagcga tgaacgtgaa agaagcaatc
     1021 caatgggtta acattgcttt catggctgtc tgccgtgtga ttaacggtgc tgctacatct
     1081 ctaggtcgtg taccaatcgt attggacatc tttgcagaac gtgaccttgc tcgtggtaca
     1141 tttactgaat cagaaatcca agaattcgtt gatgatttcg ttatgaaact tcgtacagtt
     1201 aaatttgctc gtacaaaagc ttatgaccaa ttgtactcag gtgacccaac ctttatcaca
     1261 acttctatgg ctggtatggg taacgacggt cgtcaccgtg ttactaagat ggactaccgt
     1321 ttcttgaaca ctcttgacaa catcggtaac tcaccagaac caaacttgac agttctttgg
     1381 actgacaaat tgccatacaa cttccgtcgc tactgtatgc acatgagcca caaacactct
     1441 tctatccaat acgaaggtgt aacaacaatg gctaaagacg gatatggtga aatgagctgt
     1501 atctcatgct gtgtgtctcc acttgatcca gaaaatgaag aacaacgcca caacatccag
     1561 tacttcggtg ctcgtgtaaa cgttcttaaa gcccttctta ctggtttgaa tggtggttac
     1621 gacgatgttc acaaagacta caaagtattt gatatcgaac caatccgtga cgaagttctt
     1681 gaatttgaat cagttaaagc gaactttgaa aaatctcttg actggttgac tgacacttac
     1741 gtagatgcct tgaacatcat ccactacatg actgataggt acaactacga agctgttcaa
     1801 atggccttct tgccaactaa acaacgtgcc aacatgggat tcggtatctg tggatttgct
     1861 aacactgttg atacattgtc agctatcaaa tacgctacag ttaaaccaat ccgtgacgaa
     1921 gatggctaca tctacgatta cgaaacaatc ggtgactacc cacgctgggg tgaagatgac
     1981 ccacgttcaa acgaattggc agaatggttg atcgaagctt acacaactcg tctacgtagc
     2041 cacaaactat acaaagacgc agaagctaca gtatcacttt tgacaatcac atctaacgtt
     2101 gcttactcta aacaaactgg taactcacca gttcacaaag gtgtatacct caacgaagat
     2161 ggttctgtga acttgtctaa acttgaattc ttctcaccag gtgctaaccc atctaacaaa
     2221 gctaaaggtg gttggttgca aaacttgaac tcactttcta gccttgactt tagttatgca
     2281 gctgacggta tctcattgac tacacaagta tcacctcgcg ctcttggtaa gactcgtgat
     2341 gaacaagttg ataacttggt aacaattctt gatggttact tcgaaaacgg tggacaacac
     2401 gttaacttga acgttatgga cttgaacgat gtttacgaaa aaatcatgtc aggcgaagac
     2461 gttatcgtac gtatctctgg atactgtgta aacactaaat acctcactcc agaacaaaaa
     2521 actgaattga cacaacgtgt cttccacgaa gttctttcaa tggatgacgc cttggatgca
     2581 ttgagctaat caagttcttg aataataaaa aggaaccctc ggtcaaacga ctgagggttt
     2641 tgtgcttggg atagtatgag caattccttc ggcgcaatat gcaatgtttt tgggctcttt
     2701 gtcaactgta gtgggttgaa aaaaagctaa gcttgagaaa ggacaaattt cgtcctttct
     2761 tttttgatgt tcagggcgat aaaaatccgt tttttgaagt tttcaaagtt ccgaaaacca
     2821 aaggcattgc gcttgatgtc tttgatgagt ttgttagtgg cctcaagttt agcgttagaa
     2881 taaggcaatt caatggcgtt agtgatgtag tttttatagc aaataaatgt gctcaaagtg
     2941 gttttaaagg tgcggttgag atgaggtaac gtgtcttgaa ttaagcccca aaactggtca
     3001 gtattcttct cttgtagatg aaataggagt agttgataca ggtcatagta atctttaagt
     3061 tcaggtacta gagtaaagat tttcttcaga cactccctag gagttaaggt ctctctgaaa
     3121 gttctagcat agaaaggctt aagagagagt ttccgactat cttttaggat aaatttccag
     3181 taatatttaa gagctctgta ttccagagat ttatcatcaa attgcttcat gatgttgatt
     3241 ctagtctgat taagagccct gctcatgtgt tggacaatgt ggaaacgatc gagaacaatt
     3301 ttagcattgg gaaataattt cttaatgaga gggatataac ttccagacat atcaacagtg
     3361 acgactttaa ctttttttct agcttctttc gagtacttga agaaatgatt tcggatggtt
     3421 gtttgacgtc tgttatcaag aatggtcatg attttcttag tgttgaaatc ctgagcaatg
     3481 aaagccaatt tccccttctg gtaggagaat tcatcccagg agaggatttc aggcaaagtg
     3541 gtgtaatcct cttggaaatg aaattgcttg agcttacgat agacggtaga ggtagaggta
     3601 gaggtagagg tagagatggc taatttagaa gcgatatgtg taagagcctc tctgttgagt
     3661 aggagttggg caattttctg tctcaccatt tccgagattt ggcaattttt ctgaacgaga
     3721 gttgtttcag ctacagtgac tttccgacag gacttgcatt gaaatcgtct ctttttcaaa
     3781 tgaatgaggc tagggaaacc accaatctcg ataaaaggga ttttagaagg cttttggaag
     3841 tcgtatttga tttgttttcc tttacagtgt ttacatttag gtgggtgata atcaagtgta
     3901 gcgaagactt cgatatgggt atcgtgctga atggctttat ttaaggtgat gtttttgtct
     3961 tttattccga tgagtaatgt ggtatgattg atgtgttcca taagatactt tctaatgagt
     4021 tgtttaggcg cttttcatta taagtcttat gggacttttt tgatactcaa aaagccctat
     4081 aatctccaca gtgggattta cccactacag aaattataga gccagaaaaa acacttttgt
     4141 tcactagcag aaactagaga gcagaagtgt ttttctgttc agatttaccc aaaactggga
     4201 aatatgggga taagaataga gatggcttag gaagcccctt tttgtgtgta gacagtacga
     4261 tgaacttata acaaatagtg agccttttta gcaatcattg cgacccgttt gtcaaaagcc
     4321 tcttttcgga tatctacaat tgtctgatag atgagacgct gttggctaac atgcaaatct
     4381 aaggcaatcg tcaaaaagtg atgtttccct ttgggatact gctttttaac gtaaggcagg
     4441 tattctttcg ttgtaataat aatcaatggc tctgtcaaat gctcctctga aggaggagga
     4501 ctaattagaa tattgtatcc tgtaacagag gcaactttgt cagtaaaatt ccgtaaaata
     4561 atggacttta ttaagtttac atctgcttga ttatttaaaa tgataaaaat cgggatagca
     4621 ggtagtgagg aaaagatggt ttctgtcaag tagagtgaga aaaggtacag ccgatgctgg
     4681 tcgataactc cttcaatctt ctgctcagtc atccactctt gaacaattgc tttcgaaata
     4741 tgatacagtg gcttgtcgct ttcaatccca taatgttcgt aataattata atagggaact
     4801 agattttgta aaccaaacaa aaacgttctt gttaagaaag tcagtgctgt taaaaaagaa
     4861 agagaattcg aaatgtcatt tcctaagata ttcttgaact tggatagtag atgctttcct
     4921 cttgtatgct gaagaatcag ttgaatagta tgagtctttt tttcttgatt ccatttgtcc
     4981 ttggaaaacg aagaattagc agaacaataa accaaaaaga tataatccag ttcttcctga
     5041 gtaaaagtca tgttggcatg tggctctaag taagtttggc aatgttccat caaaatcgga
     5101 tacataaaga ggttttttaa tttttcaaac tctttggact cagggaactc aagtggaaat
     5161 tcccgacgtt tccaagtgag tgccactagt atgctaaaat gaacatactc gtcaggtgtg
     5221 atttctaaca gttcatgact gagttgagaa ttagactgca caatcatatg tgtgacccaa
     5281 tccatacttc catcattcaa atcataaatc tcaataccaa aatgaaactg gaggagtgca
     5341 attaaaaaac gaatgcgata ttcaggacca actacttgat ttttcacaag gtccaaacct
     5401 actgaacgta gtaacaagcc acacttttgt cgtacgcggt agcctgttgc gatggaaata
     5461 tactcttttt gtgtaaattc gttaaagctt tgattacctt gtagtagaaa gaagcggagt
     5521 atttttaaaa tagttgattg gttataaagc tgatggaagt aataattcgt ttgatgagaa
     5581 tggtgttcga ttaattgaac ttgttgcgta tctaaattaa atgtcaactc ttcctcgaat
     5641 gtttcttgta attcctgcaa aatgcttagg agacttttag attgtaatga agttaaagta
     5701 gacagttcat ctagttcaat
//
LOCUS       DAVHEW010000109         5635 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVHEW010000109 DAVHEW010000000
VERSION     DAVHEW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.109"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..557)
                     /locus_tag="VVL53_001897"
     CDS             complement(<1..557)
                     /locus_tag="VVL53_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU4184011.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDG"
     gene            complement(715..3165)
                     /gene="phtA"
                     /locus_tag="VVL53_001898"
     CDS             complement(715..3165)
                     /gene="phtA"
                     /locus_tag="VVL53_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU4184012.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(3419..5152)
                     /gene="ptsP"
                     /locus_tag="VVL53_001899"
     CDS             complement(3419..5152)
                     /gene="ptsP"
                     /locus_tag="VVL53_001899"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU4184013.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(5158..5421)
                     /locus_tag="VVL53_001900"
     CDS             complement(5158..5421)
                     /locus_tag="VVL53_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU4184014.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT         1481 a         1190 c         1043 g         1921 t
ORIGIN      
        1 ccatcatccg ttgtataacg tccttgggct ctggctgcag caacagcatt atctgctctt
       61 gagttatgat tatgactgcg ttcctgcttc tgacgtttaa tctcttcttt tgtccgaata
      121 ttatccgcat gagctgcatc cttaaggtaa acatagtatt taccgtttac cttaatgaca
      181 taaccaccct tgatttcatt gacaatgtct gaatccttca actgataatt cggatctttc
      241 atgaggagct cttcactgat gatggcatca taaggaacct tgccattata gtaatgataa
      301 tggtctccat gagaggtcac ataaccttga tccgtaatct tgataacaat ttgttcggcg
      361 ttgatcccct ccctcttact gacttcatct ggtgtcaagt tttctgcctt ttgaccagcc
      421 tgatcaccat ctatataagc aactcgatta gactctttct tatcctgacc agcttggtaa
      481 cgtccaagct cataggaaca aacacttagg gcaaggactg ccactgaacc tgctagatat
      541 tttttattaa ttttcattct ttcctctttt cttaagtata tttgattcac tataaagtaa
      601 taaaccggtt aattaactag ttaatagttt actcttttta atattgcttg tcaagaattt
      661 tacatatata cataataaaa atgaaagcct ctttatcgag actttcattt ttcattagtt
      721 tattttttcc ttacttacag atgaaggatt acttcctttt aacaacgcaa gtaatttttc
      781 tgcttctgcc atgatactat tgttatccat aatttgaaga gtcaaattat ttcgtaaacc
      841 agctagagtt tctgttgcat tggctttcag actagaatcc gttactttcg caagcaaaac
      901 ttctgcttct ttgagttggg cttctacttt ttcagtctct acttgaggga cttctggctc
      961 agcaggtgtt tcctctactg gctcttcatc cgctttgaag ttcttatttg gatcttcact
     1021 gtggtctttc ttgcctaaca catgctcact ggcattgccc catccatcat tagaatgtgg
     1081 acgttcgtca gggtgttcta cgtagtactt aatcgtcgca aacaaatctt ccaaggtata
     1141 gccatttgga gctttgtatg tgtgatcatc aaaccaagca aatttaatat tatggtaatg
     1201 atccttatga ggaataatca aattaccgtt tttaacctca actgtatgct caaccatata
     1261 tggaagtcga acgagtggaa ttcgtttttc ccctttcaca cgattgtaaa tagctgctgc
     1321 actatctcca gttggatttg ctttaacatc tgcgtctgga gatggaggta ggataccttt
     1381 ttctttagta taggcttgag ctgcaacttt ttccttatca gaaaggctat cttttccaat
     1441 ccagtgacta tggcccatat gaggcgttac atatgcatct ccttcatcac tgattatatc
     1501 atgttcatca aaaatgtaac catctgacgt tgtatactta tcagctaatt gagcaatacg
     1561 aacttcgtct tcagtatact caatttgaga atttggtttg ccaagtcgct ctggatgggt
     1621 aattggtgct aggaatgcca ataaatcatc taccaatttt tctttattag tcgattcatc
     1681 attcaagcgt tctaataatt tgtctaaggc ttggaaatca gaattacgac ccttattttc
     1741 aaacaaggct ttatgagcct cagttaacag attatatgct ttatcataaa attcttggtc
     1801 acgaggagca acattttctt ttttagcagt taaagtgtgt gaaacactct cttgttttga
     1861 taacttgctt tcaagatttt taacagtttc agatggtaaa tctttcgcaa agacataacg
     1921 agagatgccc ttttcttcga atacatatcc ttccccaact tttcgtacca gctgactaac
     1981 caaagaagaa tttgagtcta ttttaagatt tggtgcaggt tgcgggcctg gactaggttc
     2041 cggagtcggt tgtggacttg gttgttctgg ccttgaatct ggtacccaat ggtttgaacg
     2101 ataacgaagg ggaataatac gagcgattcg ttcttccaat tcagacattt gagagtaagg
     2161 gatgaagtgg taatgatctc cgtgtggcac tgcaacacct ctagctgttc gacttgtgat
     2221 ttgtgctgga tcaaagacaa ggccatcaga ttctacatgt cgttgactca aaggcagttt
     2281 gtagagctgt ttcaagagac tatcaatgtc attactttga cttgcttgac tgttagtgtt
     2341 gctgttgttg cttgtgttag tatttgtagt tcctggattg cttacagaag gtacccagtt
     2401 tgttcttgaa gtgttatcgc tattttgtcg gcgataggtt cttgaatttg acagatttcc
     2461 tcgaccagat aggaaggctt ctgcagcagc caactcgcta gctgataact cattcttagg
     2521 aatgtaatgg taatgatctc catgaggaac gatataagca tcaccagtat cctctatgat
     2581 atcagaagca ttaaagatat aaccatcatc tgtagtatag cgtccttgcg aacgtgccaa
     2641 ggcaacagca ccatcgtttc ttggagttcc accttcacga tgttgactat gctcttgttt
     2701 ttgtcgattg atttcctctt ttgtacggac gttatccgcg tgggcagcat ccttaaggta
     2761 aacatagtat tttccatcta ccttgataac atatccaccc ttgacctcat taacaatatc
     2821 ctcatctttt agcttatagt ttggatcttt catgagtaat tcttcactga tgatagcgtc
     2881 ataaggaacc ttaccattgt aataatgata gtggtcgcca tgtgaagtga catagccttg
     2941 gtctgttatc ttgatgacga tttgctcagc attgattcct tcacgcttgc taacctcatc
     3001 aggagtcaaa ttctccgttt tttgcgtcgc ttgttttcca tctatatagg aaacacgatt
     3061 attttcctta accgttctag cttgatacag tcccaactcg taagaacaaa cacttaaaat
     3121 caaagctgcc gcagaaccaa caaggtattt cttattaatt ttcatcgaaa ctctctttct
     3181 atttgatatt tctagttgca attcatttct aaattgcaga aactcctctc tattacttaa
     3241 ctggttaata gtttactcct aaatctccaa cttgtcaaga attttatgaa ccatttaagt
     3301 attttttgtt ctgaccacta ctttttcaag gttggacttt ctataagaaa gattttcttg
     3361 aaaattcttt ttaaaatatc aaaaaaatcc ctgtaactga gttgcaggga cttttcgatt
     3421 aatcaaaatt aacgtattct ttttgaagtt caagaacttc ttccattgtt gagcattctg
     3481 taagggcacg gtttgcgtac tcttccatct tagctgtgtc gagtttcttc atcaagctgc
     3541 gtgtacgaag tacagatgtt gctgacatag agaactcatc caagcccatt ccgacaagaa
     3601 gtggaacagc ttgttggtca ccagccatct caccacacat accagcccat ttaccttcag
     3661 cgtgagctgc tttgatcaca ttgttaatca agcgtaggat tgatgggttg tatggttggt
     3721 aaaggtatga aacttgttcg ttcatacggt ctgctgccat tgtatattgg atcaagtcgt
     3781 ttgtaccaat tgagaagaag tcaacttctt tagcaaattg gtctgcaagc atagccgctg
     3841 caggaatctc gatcatgata ccaacttgga tgttatccgc aactgcaaca ccttcagcaa
     3901 gaaggtttgc tttttcttca tcaaagactg ctttcgctgc acggaattct ttcaagagcg
     3961 caaccattgg gaacatgata cgcaattgac cgtgaacaga cgcacgaaga agagcacgga
     4021 tttgtgtgcg gaacatagca tctccagtct cagagataga gatacgaaga gcacggaatc
     4081 caaggaatgg gttcatttcg tgaggcatat cgaagtaagg aagttcctta tctccaccga
     4141 tatccattgt acgaacgaca acaggtttac cgttcattcc ttcaagaaca gccttgtatg
     4201 cttcatactg ctcatcttca gttgggaagt cttgagaatc catgtacaag aactctgtac
     4261 ggtaaagtcc aacagcttct gcaccgttgt tgttaacacc ttcaacgtct tttggagtac
     4321 cgatattagc agccaactcg aagtgtttac cgtcagcagt cactgtttga gcatctttca
     4381 aaagtgccca ttcagctttt tgtttcgcat aggcttcacc agctgcttta aattctgccg
     4441 cttgttcatc tgttgggttg ataatcactt ctccagtgat cccgttaaca gcaaggatgt
     4501 caccgtcttt aacgatttca gtgatgttat ttgtacctaa tacagcagca atttcaagtg
     4561 tacgtgccat gatagctgag tggcttgtac gtccaccaat gttggttaca aaagctttta
     4621 caaagttttt gtccaattga gctgtatctg aaggagtcaa gtcatgcgca atcacaatca
     4681 cttcttcatt gatagaagct gggtttggca attttttacc aagaaggttt gccaatacac
     4741 gttttgtcac gtcgcggata tccgctgcgc gttcttgcat gtatgggttg tcttccatgc
     4801 cttcaaagat agtgataaac atatctgtaa cttctttcag acctgcttct gcattcactt
     4861 tcttcgcacg gatagtttcc ttgatttggc tgatcatttc tgggtcagca agaaccatta
     4921 agtgagcatc aaaaacttga gctgcttctt caccgagcgt acctactgct ttctcgcgaa
     4981 taacagaaag ctcgtcttgt gatgcctgta gagcggcatc aaggcgagct tcttctgcgt
     5041 ttgtatcttc gactgtaata gtctcaaatg acaaatccgg ctgaacgagt agatatgctt
     5101 ttgcaactgc aacaccgtca gatgctgcga ttcctttaag catttctgtc atttccctta
     5161 tgccaatcct tctttttcca ttgtttctga gattgcagcg atagcgtcat ctgcatctgc
     5221 accttcagct gagatagtta cgtcagcacc ttggccaaca ccaagactca taacacccat
     5281 aattgattta aggttaactg atttaccttt gtactcaaga gtgatatctg aagcaaattt
     5341 gctagcagtt tgtaccaaca atgttgctgg acgtgcgtga atacctgttt ctgccactac
     5401 gtggaaatct ttagaagcca tagtttgact ctccttttgt tctttctttt tttgagttat
     5461 atgtgataac ccttacaatt tagtattata ccatcttcta ggattttttt caagcatttt
     5521 ctggattttc cttcaatttc ctttcagata acttgctgaa aatcttctat cctagataac
     5581 aaaacacagg atatagtttc tttaaaaaca atttatagga taatcattgc tattt
//
LOCUS       DAVHEW010000110         5570 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVHEW010000110 DAVHEW010000000
VERSION     DAVHEW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.110"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..338
                     /locus_tag="VVL53_001901"
     CDS             <1..338
                     /locus_tag="VVL53_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4184015.1"
                     /translation="KYGYTKGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVG
                     INFGLEFKQGELVISVQEMITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGV
                     ILVVLGILK"
     gene            397..2691
                     /locus_tag="VVL53_001902"
     CDS             397..2691
                     /locus_tag="VVL53_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU4184016.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTVFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            2701..>5570
                     /locus_tag="VVL53_001903"
     CDS             2701..>5570
                     /locus_tag="VVL53_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU4184017.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQT"
BASE COUNT         1942 a          734 c         1171 g         1723 t
ORIGIN      
        1 tgaaatatgg gtatactaag ggttctagtc ttatccaaga aaataataca aaaggaacat
       61 tgaatcgcgt tacgagtatg gcgacagcat tagggctagt actagtgggt ggtttgattc
      121 catcaatggt tggtattaat tttggattag aatttaagca gggggaactt gttatttctg
      181 ttcaagaaat gattacaaaa ttaattccag gatttatccc tatggctttg actttattaa
      241 tgtgtaaatt aattagaaaa ggaaagaatc cggttgtact aatctttagt gttatggcta
      301 ttggagttat tctagttgtt ttaggaattt tgaagtagta gaaagtgtgg aggtggtatt
      361 tgggatatca cctccatttt ggaagagagg taaagagtga aattatggta taagaaagct
      421 gccgcaaatt ggaatgaagc cttgccgatt gggaacggtc atttaggtgg tatgatttat
      481 ggttcagcta caaaagaatg tattcaacta aacgatgaga ctatttggta tagaggaaag
      541 tcagatagaa ataatccaga ctcactattg catcttaaaa aaattcggga atatctttta
      601 gatggagaaa ttcagaaagc cgaagaattg ataaagttaa cagtgtttgc taccccaaga
      661 gatcaaagcc actatgaatt acttggggaa ctttacattg agcatataga tattcagtct
      721 tgtgctcttt cattgtatga aagagagcta gatttagata cagctatttc taatgttgtg
      781 tttgagccta atagttgtaa tttacaaata aaaagagaat attttacgag ttttaataag
      841 aatattttat gttgccgtat agtgtcatca gttcaaaaca cattaaattt aaacattaat
      901 ttgggtagaa ataaacggtt taatgacgaa gtatctaaac tggattcaag tacaatttta
      961 atgtcggcct ctgctggagg tagaaaaggt gttcagttta aagtagtatg tcattctaag
     1021 gttacggatg gtgaagtaag tgtattggga gagacaatag ttattcggaa tgctacagag
     1081 gtatttcttt atctcaaatc aatgacggat tattggggaa atatagatat ttcttctctt
     1141 cagggagaat ttagtagtat tgattacttt acagaaaaag atgaacatgt aaaaaaatat
     1201 caggagcaat ttaatagagt tgattttaaa ctagactata gtaaaggttg tcttagcatt
     1261 ccaacgaatc tacttcttga aaacactaaa aagtatagta actacttgac taacttgtta
     1321 tttcattatg gaagatatct gttaatatcg tctagtcaac cgaatggttt acctgccaat
     1381 cttcaaggaa tatggtgtga tgaattaaat ccaatttggg gttctaaata tacgattaat
     1441 attaatactc aaatgaatta ttggatggta ggtccatgtg atttaccaga agtagaatat
     1501 ccattatttg atatgctcga aagaatgaga gaaccgggaa gactaaccgc taagaaaatg
     1561 tatggagcta gaggttttac agcacatcat aatacggatg gttttggcga tacggctccc
     1621 caatctcatg ccatgggggc tgcaatttgg gtattaacta ttccatggtt atgtactcat
     1681 atttgggaac actatttata tttccaagat gagcgtattc ttacggaaca ttttgaaatg
     1741 ataaaagaag catttctttt ctttgaagat tatttatttg aggtggatgg ctacttgatg
     1801 acaggtccaa gtgtctcacc ggaaaataaa tatcgcttaa aaaatggtat tgaaggaaat
     1861 gcttgtctat catctacaat tgataatcaa attctaagat atttttgtga ttcatgcatt
     1921 ggcattgcaa aacaattagg agacaattcg gattttatta gtcgtgtgaa ggagttaaaa
     1981 aagaaactac ctaaaacaaa aataggtagt aatgggcaaa tccaagaatg gttagaagat
     2041 tatgaagaag tagagcctgg gcatagacac atttcacctc tatttgggct ttatccttat
     2101 aatgagattg atattcataa aactccggaa ttagcagaag cagctaaaat cactatcaat
     2161 aggagattat caaacgctaa ttttttatct tcacaggaga gggagcaagc gattaataat
     2221 tggttagtaa gtggtttgca tgctagtaca caaacaggtt ggagtgctgc atggctgatt
     2281 catttttttg cgagactata tcaaggtgaa cctgcttata accagattaa tggtttgtta
     2341 aataatgcga ctcttggcaa tttatttctt gaccatccac catttcaaat tgatggtaat
     2401 ttaggtttgg tgagtggaat ttgtgaatta ttagtacaga gccatcataa ttggttatca
     2461 ctaattccag ctttaccttc tgcttggtca gaaggagaag tgaaaggttt cagagtaaga
     2521 ggaggatata aggtatcgtt tgcttggaaa aatggggata taacattcct aaaattggaa
     2581 ggaggaaaca aagatcaaaa agtaagagta agaatatatg gcaaaaatac tgatgtacaa
     2641 aatattgaat tggtatttaa ttcagaaaaa attattgagt taaattttta ggtataagtc
     2701 atgaataaag aaaaaataaa aagaaaatta atcacaatat tgtttgtatg tattgggatg
     2761 ttatgttttg gattgttagc aggagttaag gctgataatc gtgttcaaat gagaacgacg
     2821 attaataatg aatcgccatt gttgctttct ccgttgtatg gcaatgataa tggtaacgga
     2881 ttatggtggg ggaacacatt gaagggagca tgggaagcta ttcctgaaga tgtaaagcca
     2941 tatgcagcga ttgaacttca tcctgcaaaa gtctgtaaac caacaagttg tattccacga
     3001 gatacgaaag aattgagaga atggtatgtc aagatgttgg aggaagctca aagtctaaac
     3061 attccagttt tcttggttat tatgtcggct ggagagcgta atacagttcc tccagagtgg
     3121 ttagatgaac aattccaaaa gtatagtgtg ttaaaaggtg ttttaaatat tgagaattat
     3181 tggatttaca ataaccagtt agctccgcat agtgctaaat atttggaagt ttgtgccaaa
     3241 tatggagcgc attttatctg gcatgatcat gaaaaatggt tctgggaaac tattatgaat
     3301 gatccgacat tctttgaagc gagtcaaaaa tatcataaaa atttggtgtt ggcaactaaa
     3361 aatacgccaa taagagatga tgcgggtaca gattctatcg ttagtggatt ttggttgagt
     3421 ggcttatgtg ataactgggg ctcatcaaca gatacatgga aatggtggga aaaacattat
     3481 acaaacacat ttgaaactgg aagagctagg gatatgagat cctatgcatc ggaaccagaa
     3541 tcaatgattg ctatggaaat gatgaatgta tatactgggg gaggcacagt ttataatttc
     3601 gaatgtgccg cgtatacatt tatgacaaat gatgtaccaa ctccagcatt tactaaaggt
     3661 attattcctt tctttagaca tgctatacaa aatccagctc caagtaagga agaagttgta
     3721 aatagaacaa aagctgtatt ttggaatgga gaaggtagga ttagttcatt aaacggattt
     3781 tatcaaggac tttattcgaa tgatgaaaca atgcctttat ataataatgg gagatatcat
     3841 attcttcctg taatacatga gaaaattgat aaggaaaaga tttcatctat attccctaat
     3901 gcaaaaattt tgactaaaaa tagtgaggaa ttgtctagta aagtcaacta tttaaactcg
     3961 ctttatccaa aactttatga aggagatggg tatgctcagc gtgtaggtaa ttcctggtat
     4021 atttataata gtaatgctaa tatcaataaa aatcagcaag taatgttgcc tatgtatact
     4081 aataatacaa agtcgttatc gttagatttg acgccacata cttacgctgt tgttaaagaa
     4141 aatccaaata atttacatat tttattgaat aattacagga cagataagac agctatgtgg
     4201 gcattatcag gaaattttga tgcatcaaaa agttggaaga aagaagaatt agagttagcg
     4261 aactggataa gcaaaaatta ttccatcaat cctgtagata atgactttag gacaacaaca
     4321 cttacattaa aagggcatac tggtcataaa cctcagataa atataagtgg cgataaaaat
     4381 cattatactt atacagaaaa ttgggatgag aatacccatg tttataccat tacggttaat
     4441 cataatggaa tggtagagat gtctataaat actgagggga caggtccagt ctctttccca
     4501 acaccagata aatttaatga tggtaatttg aatatagcat atgcaaaacc aacaacacaa
     4561 agttctgtag attacaatgg agaccctaat agagctgtgg atggtaacag aaatggtaat
     4621 tttaactctg gttcggtaac acacactagg gcagataatc cctcttggtg ggaagtcgat
     4681 ttgaaaaaaa tggataaagt tgggcttgtt aaaatttata atcgcacaga tgctgagact
     4741 caacgtctat ctaattttga tgtgattcta tatgacaata atagaaacga agttgctaag
     4801 aaacatgtta ataatttgtc gggtgaatct gttagtctag atttcaaaga aaaaggagca
     4861 aggtatatta aagttaaatt actaacgagt ggagtgcctt tgagtttagc agaagtagag
     4921 gtttttagag aatcagatgg taagcaatct gaagaggata tagataaaat aacagaagat
     4981 aaagtagtct ctacaaataa ggtagctact caaagttcaa ccaattatga gggtgtagct
     5041 gctttagcag ttgatggtaa taaagatgga gattacggac atcattcggt gactcatact
     5101 aaggcagatt ctaacgcttg gtggcaggtc gatctgggag aagagtttac ggtttctaaa
     5161 gttgatattt ataatagaac agatgccgaa cctcagcgtt tatctaattt tgatgttatt
     5221 tttctatctt catcaggaga agaagttttt agaagacatt ttgataaagt agttgatggt
     5281 ttgttatctt taaaagtacc ttctgtaggg gctaagctag tcaaaataga attaaaatca
     5341 gcagctattc cgttaagttt agcggaagtt gaagtctatg gttcaaagag aactccgaag
     5401 aaactttcta atattgcatt aacaaaagaa actcgacaga gttcaacgga ttacaatggt
     5461 ttttctcgtc tagcagttga tggaaataaa aacggagatt atggtcatca ttcagtgact
     5521 cataccaaag aagattctcc ttcatggtgg gagatagatt tagcacaaac
//
LOCUS       DAVHEW010000111         5417 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVHEW010000111 DAVHEW010000000
VERSION     DAVHEW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.111"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            51..299
                     /locus_tag="VVL53_001904"
     CDS             51..299
                     /locus_tag="VVL53_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184018.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            430..1602
                     /locus_tag="VVL53_001905"
     CDS             430..1602
                     /locus_tag="VVL53_001905"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU4184019.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1602..2876
                     /locus_tag="VVL53_001906"
     CDS             1602..2876
                     /locus_tag="VVL53_001906"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU4184020.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VVLSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2911..3876)
                     /locus_tag="VVL53_001907"
     CDS             complement(2911..3876)
                     /locus_tag="VVL53_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4184021.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3857..5311)
                     /locus_tag="VVL53_001908"
     CDS             complement(3857..5311)
                     /locus_tag="VVL53_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU4184022.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
BASE COUNT         1531 a         1098 c         1239 g         1549 t
ORIGIN      
        1 tactttcctt attgtctgta aatctactga ccttgttgtt tatcccagtc atggtttcta
       61 gttcgggctc agagtttcaa agtggatggc aagagcatca attgattgct gagaaggtta
      121 gtaaaacact tgataagaca tttgataagg atgtcagaga aattccgacc agtcagtttt
      181 atcaaaaatt tgtagatgag atgggaagga cttactcagg aaatttgatc ctccaggagc
      241 tgataactgt gaatggagct tataaagcta cttatatcgg tgaactctct agcaactaat
      301 cattctttaa aatgatgacc aataagaggc ttgatttggt gaatcaagtc ttttttctct
      361 cttttactgc catttgtgat aaaatagtat agaacgattt tttacatgaa tgataaaaca
      421 gaggtaaata tgacaatcgg tattgataag attggttttg cgaccagtca atatgtcttg
      481 aaattacaag acttagcaga agcgagggga attgaccctg aaaaattaag taaaggactc
      541 ttactcaagg aattgagtat tgcgccccta actgaggata tcgtgacctt ggcggccagt
      601 gctagtgact ctattttaac tgagcaagaa agacaagaag ttgacatggt cattgtggcg
      661 accgagtcag gaattgacca gagtaaggct gcggccgtct ttgtgcatgg cttgctgggc
      721 atccagccct ttgctcgtag tttcgagatt aaagaagcct gctacggagc gactgctgcc
      781 ctccattatg ccaaattgca tgtggaaaat tctccggagt ccaaggtctt ggtcattgcc
      841 agtgatattg ccaaatacgg tattgaaact ccaggagaac caactcaagg tgctggaagt
      901 gtagctatgt tgattacaca aaatccacgc atgatggcct ttaataatga caatgtagct
      961 cagacccgtg acatcatgga tttctggcga ccaaattact cgacaactcc ttatgtaaat
     1021 ggtgtctatt ctacccaaca atacttggat agtttgaaaa cgacttggct tgaatatcaa
     1081 aaacgctacc agcttacttt ggatgatttt gcggctgttt gtttccactt gccttatcct
     1141 aaattagcgc taaaaggctt gaaaaaaatc atggataaga gcctgcctca agagaaaaaa
     1201 gacctcttac aaaagcattt tgaccagtct attctctaca gtcaaaaggt ggggaatatc
     1261 tacacaggtt cacttttcct tggacttttg tctctcttgg aaaatacaga tagcttgaaa
     1321 gctggggata aaatcgccct ttatagttac ggaagtggag ctgtggctga gttcttcagt
     1381 ggtgaattgg ttgaaggata tgaagcttat ttggataaag accgtttgaa caagctcaac
     1441 caacgaactg ccctatccgt tgcagactat gaaaaggtct tttttgagga agtaaacttg
     1501 gatgaaacta actctgccca gtttgctggc tatgaaaatc aagattttgc cttggttgaa
     1561 attctcgacc accaacgccg ttatagcaag gttgaaaaat aatgaagata agttggaatg
     1621 gattttctaa aaaatcatac caagagcgcc tcgagctgtt aaaagctcag gcgctcctta
     1681 gtcctgagag acaagctagt ctggagaagg atgaacagat gagcgtgact gtggcagacc
     1741 agctgagtga gaatgtagtg ggaacttttt ctctgcctta ttcactggtt ccggaggtac
     1801 ttgtcaacgg tcaggaatac accgttccct atgtgacaga agaaccctct gtggttgcgg
     1861 cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg ttttactgca caagtccatc
     1921 agcgacagat gattgggcag gtagcccttt atcaagttgc taatcctaaa ctagcgcaag
     1981 agaagattgc cagcaagaaa gcggagctct tggagcttgc caatcaagcc tatccttcta
     2041 tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga gcagataaaa ggcgaaccag
     2101 actttctcgt tgtttatatt catgtcgata cccaggaagc catgggtgcc aatatgctca
     2161 acaccatgct ggaagccttg aaaccagtct tagaagaact cagtcaggga cagagtctca
     2221 tgggaatcct gtccaactac gcgaccgatt ctctggtgac tgcaagctgt cgcatcgcct
     2281 ttcgctactt gagccgccaa aaggatcaag gacgagagat tgcggagaaa attgcgttgg
     2341 ctagtcagtt tgcgcaggct gatccttacc gagctgctac tcataataaa ggaattttta
     2401 atggtattga tgcgattttg attgccactg gtaatgactg gcgtgccatc gaagctgggg
     2461 cccatgcctt tgccagtcga gatggacgct atcaaggtct tagctgctgg acgctggacc
     2521 ttgaaagaga agaattggtc ggtgagatga ccctgcccat gcctgtagcg actaagggtg
     2581 gctctatcgg cctcaaccca cgtgtagttc tcagtcatga tctactagga aatccttctg
     2641 ccagagaatt agcccagatt atcgtgtcca tcggtcttgc tcaaaatttt gcagccctca
     2701 aagccttggt aagtacgggc atccagcaag gccacatgaa actacaggcc aaatccctag
     2761 ctctcctagc tggggctagt gaatctgaag ttgctcccct agtagagcgc ctcatctcag
     2821 ataaaacctt taacctagag acagcccagc gctatctcga aaatttaaga tcataaaaac
     2881 tcagacgaat cggtctgagt tttcttgtgt ttaaatactt tttcctggta atagcgtaac
     2941 tggtaagaag taaccagttg tggcgacttc cttgccttca atcttttgca agagaagatc
     3001 aatagtgaga caagcaatct cttccaaagg ttgcttgata gtagccaatt gagggtagta
     3061 attttcgata aagtaggtcc catcatagcc gatgaccttg agctcttttg ggacagaaat
     3121 gcccaattct tgagcgattt taatgaccag aatagctgtc aaatcatccg aagcaaaaat
     3181 ggcatctggt ttttcccggg tcaagatatt cttgatttcc atttcttttc tgacgggaga
     3241 aaagtcactg gaaacattga taataggagc ttttgggagt acggatgcaa aaccagcgtg
     3301 gcgcagtccg gttggcgaat tagaattgtc attccctgta atcatgatga tagactgggc
     3361 acctgtcttg accaaggttt gggcagcaag aaccccacca gcatagttgt cagaggagac
     3421 gacagggatg tctggcgata ggtttcggtc aaaggaaata atcggcgctg tcacacgatt
     3481 gtagtcttcg attcctaggt tgtgactacc agaaatgatg ccgtccacct gattggcttc
     3541 caacatttcg atgtattcgc gttccttctc agaatcatgt tcactgttgc agatgatggt
     3601 cttgtaacca tttttgaaga gttggtgttc caatttatca atcaattctg catagaaaac
     3661 attggaaata ttggggaaaa tcaagccgat taacttagct gattttcctt gcagactacg
     3721 agccaggttg ttgggtttat agcccaattc tcgcatggct tcattgactt tttggatggt
     3781 tttctcagat agataccctt ttttattgat aacccgagaa acggtagtag gactgacgcc
     3841 tgcaagtttg gcgacatcag ttagttttgc gaccataatc taattcatag taagttccag
     3901 ttgggtttcc agatttaatc aggataccat tttggtccgc atgtgggaag acacgaccag
     3961 aaaatacttt ttctccttta ttgatgaaaa tttcaaagac agagttatcg atgaagattg
     4021 tagcagtagt agcctgattc tcgatagggc aagaacgagt tgtcccaaat tcttgggcat
     4081 actgttctcc agcctggcta cgatccactg ttacttgacc gtttacaagg tcaaagttga
     4141 ttgaaagtcc cttaccttct ttatcagcaa gtaagacaat ctcgctctgg ctattagctt
     4201 ccaagttgag ttcaagttcg taagtgttct tggtttggga acggtttgag aaggcttctt
     4261 cagaagcacg aaggtcctta atagcagcga ctggatactg gtagagcttg tcgtctttga
     4321 tagtgagttc cttgaccaaa gagaaggttc cttggtggtc aaaacggtca gatgggtaag
     4381 aaacatctgg caaaccaagc cagctaactg ctagagcacg cccatcagga gcgttgaagg
     4441 cttgagttgc ataggcttcg aaaccgtaat ccatgttttg aagttgagac acatctacca
     4501 ttttggcatt tttagggtca aaggaagccc cgatcttata catatttgga aagatattat
     4561 cgtagtctag aactttctta tccaatcctt gtggacagta gagaaggaca ggttgttcct
     4621 ctacaaagac caaattagga cattccatca tgtaggcagt acggtcgtta gcaaagtcaa
     4681 ggtcgccaac tgcttgccag tttgtgtagt cgttattgac agccttgtag agacgaacga
     4741 aacctttttt ctccaagtct tgtccgccga caatggcata atattgaccc tgaaagttaa
     4801 aaatttgtgg atcgcggaag tggtcagtag agtctgctgg ctggtcaatc aagatcttgt
     4861 caatctttgt aatcttaccc tccttgtcca tcaaagcacc gatctggtat gggtgacgga
     4921 tccagttttt atcgcgaaca tttcctgtat aaaataggaa taagttatcg ccaaattgca
     4981 tggcagaacc agagtaggca ccgtggctat ctaatggagt atctggtaaa actttgattc
     5041 cagtttcttt aaagtgaatc aaatcatcac tttctagctg tgcccaagat tttaaaccgt
     5101 gggctgcacc aaaaggaaaa ttctggtaaa agaggatcca cttgccatca aagtaagaaa
     5161 agccatttgg gtcgttgaga agtcctgttt ttggctcaac atggtaatga gtatgccatg
     5221 gagattgtgc catattttcc tttatatgtt gaatttcttc ttgcgtccaa tcttgataaa
     5281 gtctgtaacg acgctcagtt gtccattcca ttcattcctc caaaaatctt atcactttta
     5341 atagtaactg ttttcgataa aaaatgcaag ccttttatgt taaaaaagag gaaaaactgt
     5401 caaaggatta tcataaa
//
LOCUS       DAVHEW010000112         5095 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVHEW010000112 DAVHEW010000000
VERSION     DAVHEW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.112"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..82
                     /locus_tag="VVL53_001909"
     CDS             <1..82
                     /locus_tag="VVL53_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU4184023.1"
                     /translation="KSQVIELLLNTVDYFDDIMGAIYSKK"
     gene            144..902
                     /locus_tag="VVL53_001910"
     CDS             144..902
                     /locus_tag="VVL53_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4184024.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            960..1814
                     /locus_tag="VVL53_001911"
     CDS             960..1814
                     /locus_tag="VVL53_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU4184025.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            1926..2810
                     /locus_tag="VVL53_001912"
     CDS             1926..2810
                     /locus_tag="VVL53_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU4184026.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            2973..3335
                     /locus_tag="VVL53_001913"
     CDS             2973..3335
                     /locus_tag="VVL53_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU4184027.1"
                     /translation="MELSAIYHRPESEYDYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLP"
     gene            3316..4712
                     /locus_tag="VVL53_001914"
                     /pseudo
     CDS             3316..4712
                     /locus_tag="VVL53_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            4798..4965
                     /locus_tag="VVL53_001915"
     CDS             4798..4965
                     /locus_tag="VVL53_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184028.1"
                     /translation="MNCRGHETRQRIVRDFEVQPKAHIKLLANQQKHSDAGATIEDEY
                     YVSQIIHTSLN"
BASE COUNT         1656 a          812 c         1068 g         1559 t
ORIGIN      
        1 aaagtcccag gttattgaat tactcctaaa taccgttgat tatttcgatg atattatggg
       61 ggctatttac tcaaaaaaat aagagcaaaa ttgctattat tttttacaaa ttggtgtaac
      121 actagtgata taggagtgat gaaatggttg tttatattag acaaagtaaa ttaccaagtg
      181 aggtttcaat taataaatac aatgctcaag taggtgccta cttacaagga gaagaagtta
      241 ttttatatca atctttttct gaaataaaag agttaacaag tgaagatata gttgtagatt
      301 atataatgga gactagagca ttactaaaaa tgatgggctt aaacgttccg gttcatgact
      361 atcctattga gcttaaagag ttttatggtc gaaagattta tgctggtatt ttaggagaga
      421 ttgtgaatat acctgataat tggggaaaat ttattaagcc taaagctggt tcaaaagtct
      481 ttactggaag agttgttaat ggaacccatg atttaatagg tattggtcta cctttcgact
      541 atcctatatg gattagtgag gttgtagaat tcatagctga atggcgttgt tttgtgttag
      601 atggtcgcgt attagatgtt cgaccctata caggtgatta tcatgcacaa tttgatgcaa
      661 gtgtaattga tgaagcgata tcatgttgga aagatgcgcc aatagcttat ggactagata
      721 tcggtgttac tcgcgatggc agaacacttg ttgttgaagt aaatgatggt tatgcattgg
      781 gaaattatgg cctatctcct ttaaaatcaa tcaattttca tagagctagg tggaaagaaa
      841 tggtaaaacc ctattttgaa aaaaatgaaa tttttaaaat ccaacaggat gttattttct
      901 aaaataaata tggtagataa ccaatataaa aagcatcaaa atgaacaagg aggaatctga
      961 tgcttggagc aattattgga gatattgtcg gttctgttta cgaatggaac aatatcaaaa
     1021 caaaggactt tcctttattt cggaaggact gcttttttac agatgacacg gttatgacct
     1081 gtgctgttgc agaagcaatt atgaatggtg gacaaaaaga tgacttcatt gacgcgatga
     1141 agaaatatgg cagaatgtat ccgaatgctg attacggtgc tcggtttaat gcatggctaa
     1201 acagcgataa ccgtgagcct tataatagct ttgggaatgg atcagctatg cgtatttctc
     1261 catgtgcttg gatcatggac tgtggttttt atgcgaaaac tggtatatgg ccatcatcta
     1321 gaggacttac gagtctttct gcagaggtaa ctcataatca tccagaaggt gtcaaaggcg
     1381 ctatggctac agctgatgct atctttctat gtcgttttta ctttggtggt tattgtagag
     1441 agtacgagca atcaattaac gacaatccta cagagtgtaa aagacgaatt aaggattata
     1501 tagaaaaaga atacgattac aatctatctc aaactttaga tgaaatccgt cctaactatc
     1561 gttttaacga aacatgtcag gaaactgtcc ctcaagccat tatcgccttt cttgaaagta
     1621 gagacttcga agatgcgata agaaatgcca tctcacttgg tggcgacagt gatactctcg
     1681 cagcaatcac ttgtagcata gcagaggcag cttacggtat tcctgattgg atcaaggata
     1741 aggcctattc ttacttggat gaacccttaa aggatgtagt taggcgatgg gaaaatagaa
     1801 taaaagcgta ttaatctcca gtacgtcaga tttctctttt ctgtattttc attgtataat
     1861 aggattacat ttacttgcat acgacgaaat gtaaacattt ctgtttaaaa ttaaggataa
     1921 aatcaatgaa aaaagcaatg gtaattatca accctacttc tggtggcgag aaggctttgg
     1981 attacaaaga aaagctggag aataaagcaa aagaatactt tgaatatgtt gaaaccaaaa
     2041 ttaccgaaaa agcgctggat gcaacacatt ttgctgaaga agcttctcgt gagcagtatg
     2101 atgcagtggt tgtgtttggt ggagatggaa ctgtcaatga agtcatttca ggtattgatg
     2161 agagagacta cattcctaag ttagggatta tcccaggcgg tacgggtaac ctcattacaa
     2221 aacttttgga aatcaatcaa gacatcgatg gcgcaattga cgaactggat tttgatttaa
     2281 ccaataagat tgatatcggt aaagcaaatg acaactattt tggttatatc tttagtatcg
     2341 gttctctgcc tgaggcgatt cacaatgttg aaatagagga caaaacaaaa ttcggtattt
     2401 taacctatgc tgtaaatacc atgaagtctg tcatgacaga tcaggtcttt aacattaagg
     2461 ttgagacaga aaatggaaat tatgttggtg aagctagcca tgttttggtc cttttgacaa
     2521 attacttcgc tgataagaaa atctttgaag aaaacaagga cggctatgcc aacattttga
     2581 ttctgaaaga tgcctctata ttctccaaat tatccgtcat tcctgattta ttaaaagggg
     2641 atgttgtcgc aaatgataat atcgagtata tcaaagcgcg taatattaaa atctcttcag
     2701 atagtgaatt ggagtcagat gttgacggag ataaatcaga taacctacct gtagaaatca
     2761 aagtcctagc tcagcgagta gaagtatttt caaaaccgaa agaggattag tatatagaga
     2821 aagccttttt taaggctttt tgtatacttt aaaagatagt tcctttaaca acggacattc
     2881 cttgcaaata gtttacaaaa atagtatact ggattcattg agtttgaaaa cgtttgcgta
     2941 aaatttgaat gaatacttta ggagacaaat tgatggaatt gagtgctatt taccataggc
     3001 ctgagtcgga gtatgactat ctttataagg ataagaaact ccatattcga attcgaacta
     3061 agaaagggga cattgaaagc atcaacttgc actatgggga cccttttatc tttatggagg
     3121 agttttatca ggatacaaaa gaaatggtca agataacttc tggtacctta tttgaccatt
     3181 ggcaggttga agtgtcagtt gactttgcac gtatccagta tctctttgag ctcagagata
     3241 cagaaggtca aaatattttg tatggcgata aagggtgtgt ggaaaattct ctagaaaatc
     3301 ttcatgcaat tgggaatgga tttaagttgc cttagcttca tgagattgat gcctgcaagg
     3361 ttcctgactg ggtttcaaat acggtatggt atcagatatt tcctgaaaga tttgccaatg
     3421 gcaatgctct attaaaccca gaagggactt tagactggga ttcatctgtc acacctaaga
     3481 gcgatgattt ctttggtggt gatttacagg ggattattga tcatatgaat tacttgcaag
     3541 acttgggtat tactggacta tatctttgtc ccatctttga atctacaagc aatcacaagt
     3601 acaatacgac agattacttt gaaattgacc gtcattttgg agacaaggag acctttcggg
     3661 aactggtgga tcaagcgcat catcgtggca tgaaagtcat gctggatgcg gtatttaatc
     3721 atattggttc gcaatctctt caatggaaaa atgtcgtcaa aaatggtgaa cagtctgctt
     3781 ataaggattg gttccatatt caacaattcc cagtgacaac tgaaaagcta gttaataaga
     3841 gagacttacc ctatcatgtt tttggtttcg aggactatat gcctaagcta aatacagcca
     3901 atccagaggt caagaattat cttttaaagg ttgcgactta ttggattgaa gagtttaata
     3961 tcgatgcttg gcgtttggat gtggctaatg agattgacca tcagttctgg aaggattttc
     4021 gtaaggcagt tttagctaaa aatcctgatc tttatatcct aggagaagtc tggcatacat
     4081 ctcagccttg gctaaatgga gatgagttcc atgccgtcat gaattatcct ttatctgata
     4141 gtatcaagga ctatttctta cgaggaatta agaagacaga ccagttcatc gatgaaatca
     4201 atggagagtc tatgtattac aagcagcaga tttcagaggt catgtttaat ctcttggatt
     4261 cacatgatac agagcgaatc ctgtggacgg ccaatgaaga tgttcaactg gttaaatcag
     4321 ccttagcctt tctcttttta caaaaaggaa caccgtgcat ttattacgga accgagctag
     4381 ccttgactgg aggaccagat ccagattgtc gtcgttgtat gccttgggaa cgtgtatcaa
     4441 gtgacaatga tatgctgaac tttatgaaga ggctgattaa aattcggaaa tacgcgtcag
     4501 taatcatttc gcatggcaag tatagccttc aagaaatcaa ctctgatcta gtagctctgg
     4561 aatggaaata cgaaggacgg atcctcaaag caatattcaa ccaatcaaca gaagattatc
     4621 ttttagagaa agaagcagta gcactagcaa gcaattgcca agaattggat aatcagcttg
     4681 tcatctctcc agatggattt atgattttct aaaaactagt tgatgaagat tatggtacat
     4741 ttcatacctt atatagtata ataaggctag ttactaaact tgtaaaggag aacttaaatg
     4801 aattgtagag gacatgaaac aagacaaaga attgttagag attttgaagt tcagcctaaa
     4861 gcacatatta agctgttagc aaatcaacaa aaacatagtg atgcaggagc aactattgaa
     4921 gatgaatatt atgtatccca aatcattcat acctctctca actagatgta acttacaaaa
     4981 cccctgacct catgagccac tttcttcctc ctcatgaggt cagttttact ttctgctgtt
     5041 ccagtatcgt ttttcctcgc tagatttcct caaaagggca gactcctccc ttggt
//
LOCUS       DAVHEW010000113         4959 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVHEW010000113 DAVHEW010000000
VERSION     DAVHEW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.113"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(167..931)
                     /locus_tag="VVL53_001916"
     CDS             complement(167..931)
                     /locus_tag="VVL53_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184029.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1027..1430)
                     /locus_tag="VVL53_001917"
                     /pseudo
     CDS             complement(1027..1430)
                     /locus_tag="VVL53_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1610..2242)
                     /locus_tag="VVL53_001918"
     CDS             complement(1610..2242)
                     /locus_tag="VVL53_001918"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU4184030.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2276..2977)
                     /gene="pyrF"
                     /locus_tag="VVL53_001919"
     CDS             complement(2276..2977)
                     /gene="pyrF"
                     /locus_tag="VVL53_001919"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU4184031.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            3296..3388
                     /locus_tag="VVL53_001920"
                     /pseudo
     CDS             3296..3388
                     /locus_tag="VVL53_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3723..4436)
                     /locus_tag="VVL53_001921"
     CDS             complement(3723..4436)
                     /locus_tag="VVL53_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4184032.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSFSLAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEEFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(4429..>4959)
                     /locus_tag="VVL53_001922"
     CDS             complement(4429..>4959)
                     /locus_tag="VVL53_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4184033.1"
                     /translation="YNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALIN
                     NPAVLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNK
                     GKLVEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQTDLENIFSIHSCEP
                     TLEDIFITLTGGKLNA"
BASE COUNT         1650 a          988 c          838 g         1483 t
ORIGIN      
        1 atatcttgaa gtaaaactaa gatccattct aatacatttg gatggattga atattggcga
       61 cagcgcaata aatatactgt actagataaa acacaggata gcagtaatat aaaataaacc
      121 aatactgcta agaaatcttt tttataaaat tgaacaaatt gtttcattat acatagtcct
      181 ctgaatgtag aaaaaatgta ccataaacaa ccaaacaact aacaaataaa ataaaagcaa
      241 gatgagcact aactaaggaa agactgatat ctttctgata tcccaaagct agcgttgtca
      301 caggttctaa gtaagatagc cctaaaatag cccaaaaaat accaccaacc atcatatagg
      361 caactgggat gaaaatagct cctatttttt tcttcactag caaagcacta gctagtccaa
      421 aaatagagaa cacagcgccc caaactccat accagagagt cgttacaaga ctatagagaa
      481 actgattgga atcaaataat tcttttaagg caccactata atctccaata taaatttcct
      541 gataaggagt cactaaaaga ttaattccta ataataataa ataggggaga aaaaagacta
      601 gaaaagaaga aataattaca gtactaccca caatagccag atacttcttt ttagaaattc
      661 gacacaattg tgctgttaga aaatgactct cagcatcctc tattatctga ctagaatagg
      721 gcagtgtaca gataagtgca gctactaggc taatcggtga aaatcctcga atagaagaag
      781 cggcaaaaaa aatcgataaa ccttcaattt tataaatacc attgaaagca aggaaatttc
      841 ctaaactcat gcaaagaagg gtcaaaaata aagacatata aaatcgaggt gattgaacga
      901 ctcgtacaag attacaaatg aaaaattcca tccttactcc tccttataat aaaaataggg
      961 tgtagcattc ttttttcatg ctacacccac aatcaaccat ctttaaggct tactctgaca
     1021 agtaagttaa taagaatctg gattccaaga acctgaagta tgaattctta catgatttcc
     1081 aaattgtggc gccatagcta atctagtacc agaaccaata taattgtcac cacctccatt
     1141 atagtacatg acaatcctag agccagaccc caatgaatat accggggtaa tatctgaccc
     1201 actataggcg ctacgaatag aggtatttaa ccttttaccg ccaccagtgc tgtcattatt
     1261 attaattcca gcagaggagt tttctttctc aagctcacta gttgtatccg aaccccaacg
     1321 cggcgacgtt aaatcataat ctccaatctg tctagctaaa actgtaggga gaaacaaact
     1381 agatgcattt gtaactacgc aaaaaaggaa tatatttttc ttcttttcat gacatcgtcc
     1441 tcttaagaag tgataaacaa gatagttgat tgatcaattt gatacatatt acattttcat
     1501 tatatcaaca aaagaaagcg ttgtcaatta ttttatttaa aatattgtca tattttaaat
     1561 aaaatggtaa aagagactcg gtagcctcgt ctctctttat cttactgacc taaccttctt
     1621 gccaattttc ttggtcttct ttaaagcgtt ttagaagatc aaggccctct ggcgtgatgt
     1681 aaccttcttc ttgggctaga tggataagct cgctatagtt tgaaagcgtc acaagtttaa
     1741 caccagcatc tgcaaagttc ttatctgctt ttggcaattg gtagctgaaa atcgctacaa
     1801 ctccaagtac atctgctcct tctcgcttgg ctgctgctac agcttcaaga actgaaccac
     1861 ccgttgaaat aaggtcttca accactacca ttttttgacc ttgagctacg cgaccttcga
     1921 tttgattacc agctccgtgg tcttttggtt tactacggat gtaggcaaaa ggcaagtcca
     1981 tcttatcagc aataatggct ccgtgtggaa tccctgctgt tgcagttcct gcaatcactt
     2041 ctacttcagg aaaggcttct ttgatagctt ccacaaaacc attttcaatt agggtacgag
     2101 tttctggata ggctagtgtc acacgattat cagtgtaaat cggtgacttg ataccagatg
     2161 cccaagtgaa gggttcctct ggtttgaggt aaacggcttg gattttcaag aggtggctag
     2221 cgatatcttt agcaagtgtc atggtattct ccttttattt ttctaatcta gttctttaat
     2281 tccagtcctg tgtccattca tccttgatgg catgataagc tgcaacagga tcctcagctt
     2341 gggtaatggg acgtcccact acgatatagt cactgccgat ttgataggca tcagcaggtg
     2401 tcatgactcg tttttgatct ccaactgcaa caccagctgg acgaatccct ggtgtcagac
     2461 agataaaatc tggattggta gcctgcttga tgacttgtac ttcctgagcc gagcaaacaa
     2521 caccatccaa gccagcttca gctgtcttct tggcatagtg aatcacagac tcttgcagac
     2581 tggtttggat attttgaaac tcctgcatct gagcttctga cgttgatgtg agctgagtta
     2641 cagcgatcaa tttggcttga ctcccaagac cttcacgcgc cgccttcatc atctctacac
     2701 caccagccgc atggacatta gtcatatcga caccaagctg agacaagatc ttcatggctg
     2761 acttgactgt attaggaatg tcatgaagtt tgagatccaa aaagacacta tgacccaaac
     2821 cttttaagta ggacacaatc tcaggccccg ctgcgtaata aagctccatc cctaccttga
     2881 gataaaggct ttcttctgct gggaaaagag ctaaaaattc cttgaccgcc tcaaaactag
     2941 gaaaatcaag agcaatgatt ggacgatgtt ctcgcatagg tttctccttt tacctttact
     3001 agtgagagag tctggccaac agaagccacg aaaaaaggaa cctgccaaca gcaaggttcc
     3061 acgaaaaatg ggtagtctcc accctttcac cgtctaacct tagctgcctc tctggactgc
     3121 tttaaaggtt ttttactatt ctattatttc tactagcact tgtcaagtaa aaacagctaa
     3181 atccatgttg actgctattg aacacctcac aagactcttt ccaaaagaat agttaaagac
     3241 tttcacttcg gacagagaaa aagagtttgc ttgttaccct ttaattgaac aaatgggaat
     3301 tctgttttat tttgcggatg cctattcatc ttggcaaagg ggaagtaatg agaatgcgaa
     3361 tggacttctt cgtgagtatt ttcttaaacc gatttggcaa caattaccga aaaaatcctt
     3421 aatgatgctt tgctagctta ttaatcaccg tccccaaaaa tacttggatt acaagacagg
     3481 ttttgaagca ctttttgaag cacttataga ggagatttaa aaatgttgca ttttttattg
     3541 caatttatca gttatcttat cacccaaaac ttctgcaatt agtatgagca ctattgttat
     3601 ttttcttttt tcgtacactc aaatcaaaat atagcaataa gactattctg tagaattgct
     3661 gtcaaaaaga tcagttcatt ttttccttcc cacttgtgtt tataatcatt aaaataactc
     3721 aattaagaaa ttttcttttt cctaatagtt atcgtaatta aagttaatag aagaacaatc
     3781 caaataagta gtgttagatt aggaattaga gttttattcc atgaaaattc ctcccatttt
     3841 gtgaaaaact tagtaaatag tcccataaaa ctataatctg ttatcttcgc aactgtctta
     3901 tccaaaccag atagcatcgg taagaaagca actaaaatca tagcaggaag actgataacc
     3961 tgagctacta cttggctctt cgcggttaaa cctatcaata aatagaataa aatgatggat
     4021 aaagaggtta gaagaacgat tgtaatataa ttaaaagtat gtacaattgt aactcctaaa
     4081 ataagaggag tagttcccat aatcacaaaa gttagcaaaa aaggaagaaa catagttgat
     4141 aaaatgtatt cggagccttt aacaccactc aacagaagag tttgtaaatt gtacttttct
     4201 ttttcttcag acaagataat agttatagga cttccaacag ccaaagaaaa agaaaaaggt
     4261 aaacacatca tcaaaagaac taatgcctgt tgatcgttga ccttcccctg tgtttccata
     4321 agatatttat aaaaatatgt gaaagcaaaa ggcactaaaa cttgcaataa aatactctta
     4381 ttggaaagga tgatttgaca acgcaaccat accaaagcca gaaaccgttt aagcatttag
     4441 ctttcctcct gttaatgtga taaaaatatc ttctaaagta ggctcacatg aatgaattga
     4501 aaaaatattt tccagatcag tctgagatac ctgttctagt gatgtaaaat caaaagttat
     4561 ctgattccca ttataatctg taaccttaat ctttttatct ttattatatc tttgaatgag
     4621 ttcagaagga gctccttgct caactaattt ccctttattt aataaggcaa cataatcaca
     4681 taaaagagtt gcttcattca tatcatgagt cgttagaaaa atcgttgtcc ctgctgtttt
     4741 caattctaaa attaactcat gaattgttcg agaagttgtg ggatctagac ctgaggtcgg
     4801 ttcatccaga aagagtacag cggggttgtt gataagagct cgtgctaaaa gcattcgttg
     4861 cctcattcca gtggataatt ttcctgctac catcttgcga ctatcatata atcctactcg
     4921 ctttaacaaa ttatcaacac gtgatttact aatattata
//
LOCUS       DAVHEW010000114         4787 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVHEW010000114 DAVHEW010000000
VERSION     DAVHEW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.114"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(364..1092)
                     /locus_tag="VVL53_001923"
     CDS             complement(364..1092)
                     /locus_tag="VVL53_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4184034.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1250..2659
                     /locus_tag="VVL53_001924"
     CDS             1250..2659
                     /locus_tag="VVL53_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU4184035.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2677..3972
                     /locus_tag="VVL53_001925"
     CDS             2677..3972
                     /locus_tag="VVL53_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4184036.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3977..4285
                     /locus_tag="VVL53_001926"
     CDS             3977..4285
                     /locus_tag="VVL53_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4184037.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4282..4590
                     /locus_tag="VVL53_001927"
     CDS             4282..4590
                     /locus_tag="VVL53_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4184038.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1615 a          812 c          889 g         1471 t
ORIGIN      
        1 aaattgggag attgaaattc atttctagca atttgttaga agtcacagtc tactattcta
       61 accacagtat actatatgtg ttacaagaaa agactcaaaa acttctcgtc agtgtaagaa
      121 tgtttgaaac actatttaaa actaaataac tataagaagt agatcaggtg aacaatttca
      181 gtacctactc tataagctat acgaagatat ttttcaacat gatggataca acgatcactc
      241 ttttaaagcc atcttattta gaaaagagaa accatttaag catagatggg accttcttgc
      301 cctgttttaa atttttctta aactagagtc tgtaaaagat tggcttcatt ttcacatttt
      361 ctcctaggag gaatgtcgta atgcaaaaga attaaattca aataaatcat agcgatgacg
      421 actaatcgag tactcaaacg gagttccgtt atccaaataa gccacttgtt ctacttcaaa
      481 taccggttcc gttggaagga gctgcaagta atgttgctca ttttctgaac tagaagaagc
      541 tcgtaagatt tttgtagcac tggcaatatg caatcctaac tttccttcaa tgtaattgta
      601 aatcgatttt tgtaaaatat cttcagtaat acctggaata acatcggtac tcatataagt
      661 ttgttccaga acataaggtt taccgtcaat aatacgcagg cggtaaatat tataaactgg
      721 actctgcaaa gcgacctgta gtttttctgc taaaaattca tttgcaaatt ctaatttgaa
      781 gtgaataatc ctcgagtcaa ttgtagaatg agcatcttca gatatttttg tcagtccccg
      841 gatatctctt tctggaacga ttaattttct ttttgagctg ccacgagaga gaacaaaggt
      901 tccctgccca cgttttctaa aaattaagcc ctcagaaact aacaagtcta aagctttttt
      961 gatcgtcatt cgactacagg caaactgttt agctaatgtc atttcatcag gcaattgttc
     1021 gttcatggta tatttattac aaagaatctt ttcacgaatc tcattgtaaa taaataaata
     1081 ttttgtactc atttaatctg gttctctcta taatattcta atagtagtat aacaaagttc
     1141 aagaaatgag gcaaacacaa gatatgtatc aacaattttt aaaaatgtct agacaaattt
     1201 taaaattatg ctatacttta tgtatagaat aatagaaagg aggttgagca tgaaaaaaaa
     1261 gttagtattt cctaatctgt tttggtgggg agctgcttct agcggacctc agacagaagg
     1321 tcaatatgga aaagtacatg aaaatgtgat ggactactgg ttcaaaacgc atccagaaga
     1381 ttttttcgat aatgtcggac ctcttgtagc cagtaacttt tttcatactt acaccgaaga
     1441 tttccacttg atgaaggaaa ttggagttaa ttctttccgc acttccatcc aatggagtcg
     1501 actcatcaag aatttagaga caggtgagcc tgatccaaaa ggtattgctt tctacaatgc
     1561 catcattgaa gaagctaaaa agaaccagat ggatcttgtg atgaatttac atcattttga
     1621 tttaccagtg gaacttcttc aaaaatacgg tggttgggaa agcaaacatg tagtggagtt
     1681 attcgtgaag tttgccaaga ctgctttcac atgctttgga gataaggttc attactggac
     1741 aactttcaat gagccaatgg tcattccaga agcaggatac ttatatgctt tccattatcc
     1801 aaatctaaaa ggaaagggaa aagaggccgt acaagtcatc tataatctaa accttgctag
     1861 tgcaaaagtg attcaactat atcgctcatt aggacttgat ggaaagattg ggattatttt
     1921 aaacttgaca cctgcttatc caagaagtaa ttctccagaa gacttagaag caagtcgatt
     1981 tacagatgac ttctttaaca aagtcttctt gaatccagct gttaaaggaa ctttcccaga
     2041 aagattggta aaacagctag agagagatgg cgtgttatgg agtcataccg aaaaagagct
     2101 tcaactgatg aaatcaaata cggttgattt tcttggagta aactactacc atccaaaacg
     2161 tgttcaagca caagcaaatc ctgaggaata tcagacgccc tggatgccag accaatactt
     2221 caaagagtat gaatggccgg agcgtcgcat gaatccatat cgtggttggg aaatttttcc
     2281 gaaagccatt tatgatattg ctatgattgt gaagaaagaa tatggtaata ttccatggtt
     2341 tatcagtgaa aacggaatgg gtgttgaaaa cgaagcacgg tttatcggtg aaaatggagt
     2401 tatcgatgac gtgtatcgta ttgaatttta tgaagaacat ttaagatggc tacataaagc
     2461 cattgaagag ggaagtcact gttttggata ccacgcttgg accgcatttg attgctggtc
     2521 ttggaataat gcatataaga atcgttacgg atttatctcc gttgatttag aaacgcaaaa
     2581 gagaaccatc aagagatcag gaagatggta tcgcaaagta agtgacaata acggttttga
     2641 agtagaaatt gaggagtaaa gagaggagat attcagatga aaaactcaaa atttatagat
     2701 caatttgcca cctttgctgg taaactaggg aaccaaattc atttaaaaac cctaagagat
     2761 gcattcgtaa cagtaatgcc attatatatt ttggcaggtt taatcgttct tttgaacaac
     2821 acggtattta agtggatttt ccaaggggat acattaacaa gattccaata ttggggaata
     2881 acaattgcaa acggtacttt aagtatttca ggtatgatta ttgctgtaat ggttggttat
     2941 ttcttagcta aaaacagaga tttcgaaaac ccgttagcag catcaatgct atcattagtt
     3001 tctttaattg tgatgatgcc aaatacagtt tctgtagttc ctgacggagc aaaagatgcg
     3061 gtaaacattt caggtgttct ttcattcaac aacacaggta caggcgcaat gttcgccggg
     3121 gttatcgtag cgattattgc aacagaatta ttcattgaat tatcaaacgt taaagcttta
     3181 caaatgaacc ttggtgaaaa tattccacca gctgttagta gatcatttag cgtattactt
     3241 ccagtcatga ccgtcatctc cttatttggg gttgtttcag cattattatt caatataact
     3301 ggaatgaact taatctcaat cattacaatc tttattcaag aaccaattcg tcatattggt
     3361 acaagcttaa tcggggtcat tattatttac tctttaggaa atatgttatg gctatttggt
     3421 attcaccaag cagttattta cagtgccatc ctagaaccat tactattaat taacattact
     3481 gaaaacatca ctgcagcaaa taatggacaa gccattccac acatcatcaa cctatcacaa
     3541 atacaaacat tcgctttaat gggtggtagt ggatctacat tatgtttatt aatagcaaca
     3601 ttcttagtga gtcgcaatgc tgtctctaaa aacgtggcta aattatcttt tggacctggt
     3661 atcttcaata tcaatgaacc agtattattc ggttacccaa tcgtttataa catttcatta
     3721 gctattccat ttatcacagt tccagtcctt ggtattttaa tcagctactt agcaacagtt
     3781 acaggattca tgagtcctgc atttatacaa gttccttgga ctacaccagt attcttaaat
     3841 gcatggttag caacagcagg ggacgtgaga gcagttctag ttcaattcat catctttgca
     3901 cttggagttc ttctatacat tccatttatc aaagttaatg acaaagttgt tgaacaagaa
     3961 atggaaggtt aagattatga aacaaatttt attagtatgt aatgcgggaa tgtcgacaag
     4021 tatgcttgtt aaaaaaatgc aacaaagtgc gactgaacgt gggattgaaa tttctatcca
     4081 agcaaaatca atgacagaag ctaaaaagaa tatccatgaa gcagatgtca ttctaattgg
     4141 gccacaaatt cgttatgagt tacttgctgt gaaagaaatt gcaggaaata tccctgtaga
     4201 taccattgat atgcgggatt acggaatgat gaatggagca aaagttctgg aacaagcact
     4261 tgcatggata ggagaaataa gatgacagaa gaaatggaat atatctgttt tcagctcatt
     4321 gccaatagtg gagcagccaa atcatccttt attgaagcca tccaactagc taaggcagga
     4381 aatttaaaag aagcgaaaat taaagttgaa gaagcggaag actccttagt agaagcgcac
     4441 aagattcact ctaatctcat acaaaaagaa gctactggag aaaaaatagg attctccttg
     4501 ctatttatgc atgcagaaga ccaaatggct tcaacagaga ttattcagtt gctatctaaa
     4561 gaattcatcg atctctatca aaataaatag gaacactgtc attcccatct gtctttatta
     4621 atgacggcta cattgtagga acctccaaaa gttggacaaa atgattgaaa ggattggatc
     4681 ctgcattaca caagatccaa tccttttctt ttactctagt ttttataatg tgttgaattt
     4741 agaatagtcc actgcagttt ctaaaaaatt gctggaaata gatgtta
//
LOCUS       DAVHEW010000115         4738 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVHEW010000115 DAVHEW010000000
VERSION     DAVHEW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.115"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            35..250
                     /locus_tag="VVL53_001928"
     CDS             35..250
                     /locus_tag="VVL53_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184039.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(359..1099)
                     /locus_tag="VVL53_001929"
     CDS             complement(359..1099)
                     /locus_tag="VVL53_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4184040.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(1099..2664)
                     /locus_tag="VVL53_001930"
     CDS             complement(1099..2664)
                     /locus_tag="VVL53_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU4184041.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPILLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            2802..4691
                     /locus_tag="VVL53_001931"
     CDS             2802..4691
                     /locus_tag="VVL53_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU4184042.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSTFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
BASE COUNT         1317 a          867 c         1102 g         1452 t
ORIGIN      
        1 ttaaatatta taaagaagaa aataggagaa aaacatggct aagtcaaact ttgaaaaagt
       61 agaatcagtt gttggctggg ttcgtgataa gaaaatcaca ggctaccgta tctctaaaga
      121 aacgaatgcg cgtgaaatgt ctatcattgc tctggcgcag ggtcgtgcaa aagtaaaaaa
      181 tatttcattt gaaacagccc taggcttaat tgatttctat gaaaaaaatt atgaaaaatt
      241 tgaagattaa tctttggata acggcggatt cttgaccttc aagtagtaga gatagagaat
      301 ctgccttttc attttgagga cagcaaaaag actgcacggt tgatgcagcc ttttcttttt
      361 atttgagata gcgttgaagg aactcttttg ttcggtcttc tttaggattg gtgaagaggt
      421 cttctggttt accttcttca gcgatcacgc ccttatccat aaagataaca cggtgagaga
      481 catcacgggc aaattccatt tcatgggtta cgacaatcat ggtcaagcct tcctgagcca
      541 ggtcctgcat gattttgagg acttctccaa ccatttctgg atcgagagct gatgttggtt
      601 catcaaagag aatagcgtcc ggattcatgg agagggcacg agcgatggcc acacgttgtt
      661 tttgaccacc tgagagttgt tttggtttgg cttgccagta gcgttctccc atgccgacct
      721 tttccaggtt ttctttggca atcttttcag cttctgtgcg ttcgcgtttt aggacagttg
      781 tctgagcgac gattgtgttt tcaagaacat tgagattttc aaagaggtta aaggattgga
      841 aaaccatccc caacttttca cggtattgcg tgaggtcata gcctttttcg aggacgtttt
      901 gtccatgata aaggatttgt ccatcagttg gtgtttcaag taggttaatg gagcgtagga
      961 aggtcgattt tccgcttcca gagcttccga tgatagagat gacctctccc ttgtggacag
     1021 tgagtgaaat gtcttttagc acttcgtttt gtccatagga ttttttgagg tgtttaattt
     1081 caaggattgc ttgtgtcatt atttcaaatc ctccgtttgc atttggttag cacctgtagt
     1141 ataggtatcc atgtccattc ggcgctcgat aaatcgtagg atacgtgtta cggtgaaggt
     1201 gaggacaaag taaatcacgg cgatgattgt aaatgtctgg aagtattgat aggtttgtgt
     1261 tgccacggta tttcctgaga aataaagttc gacaacagag ataacgttca atacagatgt
     1321 atctttgata ttgatgacaa attcattacc agttgcaggt aggatgttac ggactacctg
     1381 aggtaggaca atcttacgca tggtctggtt atgggtcata ccaagagcag tcgcagcttc
     1441 aaattgtccc ttgtcaactg ctaggatacc accacggacg atttcagtca tgtaggcacc
     1501 ggtattgatt gaaacgatga agatagcagc cagtgtacgg tcaaggttga tcccgaaagc
     1561 ttgggcagtt ccatagtaga taaccatcga ttgaacaatc attggcgtac cacggaaaat
     1621 ttcaatgtag acattgagaa cccagccgac tagtttttga aggccataaa tggctttgtt
     1681 ttcagagaga ggagcagtac ggaagacacc aatggcaagt ccaataatga gacctatgat
     1741 ggttccgacg atagagatta aaagagtgat accagcacca cgcaagagtt gttgccagtt
     1801 ttcagaaaga attttagcga cttggctaaa gaaactactg ctagtctctt cagttgttgt
     1861 agcttcggca ggttgttcct tgatcatacg atccatcaag gcaacttggt catcttttga
     1921 aatggtttca atgctggcat tgatttggct aatacgattg tcatttttac gaagcccgat
     1981 agcgatagct gtatcttctt ccccagtttt gaaaccaggt tctacttgaa tcatcttgaa
     2041 cttagagttc gcagcttcag cagtcagtgc ttctggacgt tcagaaacat aagcatcaat
     2101 gacaccagcc tcaagagctt ggcgcatttg agcgaagtct cccatggctg tttctttttt
     2161 agcacctggg atttgtgcaa tcaagtcgta gaggtagaca ccttgttgag aagtgatttt
     2221 cgcaccatta aagtcatcca aagatttagc acttgcgtag gcagaatctt ttttgacaag
     2281 caaaattggt tcgctagtgt agtaactgct cgaaaaggca atttcttgtt tgcgttctgc
     2341 agttggactc atacctgcga taatcatgtc aatcttacca gaagtaaggg cagggactag
     2401 accttcccac ttggttttaa caaccaaagg ttctttacct aagtccttag cgattttctt
     2461 ggcgatttga acatcgtatc cgttggcata ctgattggtc ccatcgattt tgacagctcc
     2521 gttgctatca tcatcctggg tccagttaaa gggagcatat gctgcttcca tcccgatgcg
     2581 taaatattca tcggcttgag caacattgac aagtcctagc atcagcaaga gacttgtgaa
     2641 aatagatagg tatatttttc tcatgatttc tcctattctg atctattaaa aaataactgt
     2701 ctcctatttt atcgaaaaat gcgtaatttt tcaacataag taagccttta cttacgaaaa
     2761 gatgctataa tgataagaaa gataaaaagg gggcttagtt gatgaaaaaa acttttttct
     2821 tactggtgtt aggcttgttt tgccttcttc cactctctgt ttttgccatt gatttcaaga
     2881 taaactctta tcaaggggat ttgtatattc atgcagataa tacggcagag tttagacaga
     2941 agatagttta ccagtttgag gaggacttta agggccaaat cgtgggactt ggacgtgctg
     3001 gtaagatgcc tagcgggttt gacattgacc ctcatccaaa ggttcaggct gcgaaaaacg
     3061 gtgcagaact agcagatgtg actagcgaag taacagaaga agcggatggt tatactgtga
     3121 gagtctataa tccaggtcag gagggcgaca tagttgaagt tgacctcgtc tggaacttaa
     3181 aaaatttact tttcctttat gatgatatcg ctgaattaaa ttggcaacct ctgacagata
     3241 gttcagagtc tattgaaaag tttgaatttc atgtaagggg agacaagggg gctgagaaac
     3301 tctttttcca tacagggaaa ctttttagag agggaacgat tgaaaagagt aaccttgatt
     3361 atactatccg tttagacaat cttccggcta agcgtggagt tgagttgcat gcctattggt
     3421 ctcggaccga ttttgctagc gctagggatc agggcttgaa agggaatcgt ttagaagagt
     3481 ttaataagat agaagactcg attgttagag aaaaagatca gagtaaacaa ctcgttactt
     3541 gggtcctccc ttcgatactt tctatctcct tgttattgag tgtctgcttc tattttattt
     3601 atagaagaaa gaccactcct tcagtcaaat atgccaaaaa tcatcgtctc tatgaaccac
     3661 caatggaatt agagcctatg gttttatcag aagcagtcta ctcgacctcc ttggaggaag
     3721 tgagtccctt ggtcaaggga gctggaaaat tcacctttga tcaacttatt caagctacct
     3781 tgctagatgt gatagaccgt gggaatgtct ctatcatttc agaaggagat gcagttggtt
     3841 tgaggctagt aaaagaagat ggtttgtcaa gctttgagaa agactgccta aatctagctt
     3901 tttcaggtaa aaaagaagaa actctttcca atttgtttgc ggattacaag gtatctgata
     3961 gtctttatcg tagagccaaa gtttctgatg aaaaacggat tcaagcaaga gggcttcaac
     4021 tcaaatcttc ttttgaagag gtattgaacc agatgcaaga aggagtgaga aaacgagttt
     4081 ccttctgggg gctcccagat tattatcgtc ctttaactgg tggggaaaag gccttgcaag
     4141 tgggtatggg tgccttgact atcctgcccc tatttatcgg atttggtttg ttcttgtaca
     4201 gtttagacgt tcatggctat ctttacctcc ctttgccaat acttggtttt ctagggttag
     4261 ttttgtctgt tttctattat tggaagcttc gactagataa tcgtgatggt gttctaaatg
     4321 aagcgggagc tgaggtctac tatctctgga ccagttttga aaatatgttg cgtgagattg
     4381 cacgattgga tcaggctgaa ctggaaagta ttgtggtctg gaatcgcctc ttggtctatg
     4441 cgaccttatt tggctatgcg gacaaggtta gtcatttgat gaaggttcat cagattcaag
     4501 tggaaaatcc agatatcaat ctctatgtag cttatggctg gcacagtacg ttttatcatt
     4561 caacagcaca aatgagccat tatgctagtg tcgcaaatac agcaagcacc tactctgtat
     4621 cttctggaag tggaagttct ggtggtggct tctctggagg cggaggtggc ggcagtatcg
     4681 gtgcctttta aagagagcta ccatagactg aaaaagtatg atataatgga agatagaa
//
LOCUS       DAVHEW010000116         4554 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVHEW010000116 DAVHEW010000000
VERSION     DAVHEW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.116"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(366..917)
                     /locus_tag="VVL53_001932"
     CDS             complement(366..917)
                     /locus_tag="VVL53_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4184043.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1267..2091)
                     /gene="nadE"
                     /locus_tag="VVL53_001933"
     CDS             complement(1267..2091)
                     /gene="nadE"
                     /locus_tag="VVL53_001933"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU4184044.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2088..3548)
                     /locus_tag="VVL53_001934"
     CDS             complement(2088..3548)
                     /locus_tag="VVL53_001934"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU4184045.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3664..4152)
                     /locus_tag="VVL53_001935"
     CDS             complement(3664..4152)
                     /locus_tag="VVL53_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184046.1"
                     /translation="MKNRFYYSQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYGRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4142..4351)
                     /locus_tag="VVL53_001936"
     CDS             complement(4142..4351)
                     /locus_tag="VVL53_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4184047.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1315 a         1046 c          835 g         1358 t
ORIGIN      
        1 tagttttctc atcagaatga gactatttta atattagata atttgataga aaaaacatga
       61 ataaaattgg ttgaatttaa ttccctaact cgatagagga ttctatttta aatttaacaa
      121 cagctaggat tacactataa aaagtacatt accacctgtt cggctggaga atctagtcga
      181 ttagctccct cttagttaca agcttaaagc gattttttaa ctaacactaa accagattaa
      241 agaaggaact agaacatctg tcttgtacta atcaatatgc tattttaaag tttcaaataa
      301 acttggaatg ctaattatta tatttacaat aaatagttga cttcgataga aacagaaaat
      361 agaattcaac attcttccct acttatacca taataaataa gatccgcaag atagcctttt
      421 ctctctacac cattaacaat ggtttgtaga taggacattc cagccttttc catgacacga
      481 cctgaagctg ggttgagact ggcataacgt gctctgacct tttgaaaacc tgcttgagta
      541 aaacaaaagt ccaagatagc tttcaaagtc tctgtcatca taccatttcc ccagtaagcc
      601 ttgcctaaca catagccaat ttcacagctt aaatcagcct cgtctatctt aacaatgctg
      661 atatctccta ttacttgctc tgggttttct tttagacaaa tggcccattt ataatagttg
      721 agattagtat aggaagcaac ccaattgcaa atcgagtttc gagtgatttc gacatcagga
      781 tggggatccc aggtaacata ggtcagattc tcagcggatg aagcccaatt ttgaaacatg
      841 gcttctgcat cactctccac aaatcttctt aaaatcaaac gttctgtctg taatatttgc
      901 gtaccgatag atttcatgat gctacctcac ttccttattt ctatactcgt agtaaaaagg
      961 ctcaactcag taacagctag aaaacgagtt tgtcttctaa tttcttgacg cagttgctgc
     1021 ctggtttgaa atgcgctcta gcaagctttt tcaaacctag tcatcgttgc aggggtaaga
     1081 cgatgaaatc gaactcttcg agttactgaa tctgcctcat tttcttattt ctaaactcgg
     1141 ggttcaagat cccccagacc ttctctcact ttcttatttc aagatgagag gctgaaaacc
     1201 tccagtggag gtttttcttg ctttttaatt tcagggcaag aagctaaaaa ggtcccccgg
     1261 acctttttac tcccaaaagt catcaaatac ggtgatgggt aagtggcgtt tgtgttggcc
     1321 tttgtgccac cagttttcaa tggtcgcttg agcttctggg ctgattgttt tgccttctag
     1381 gtagtcgtca atctctgcgt aggtgactcc aagtgcgact tcgtcagcta ggcctggttt
     1441 atcttcttct aggtctgccg ttgggatttt ttcataaagg gctggctctg cgccaagttt
     1501 ctgcaagagc tgttttcctt ggcgtttatt gaggcggtaa agagggagaa tatccgcacc
     1561 gccgtcacca aacttggtaa agaaacctgt gatattttcc gcggcgtggt ctgttccaat
     1621 gaccgctccg ctatgggaac cagcaagggc atactgagca atcatacggc aacgtgcctt
     1681 gatattcccc ttgttgaagt ctgaaacagg actacctgtc gcttcaactg cagctgtcat
     1741 ggcatcagct gattccttga tattcacaac caagctgaca tctggctgga tgaaggctag
     1801 ggctttttga gcatctgctt catcagcttg cactccgtat ggcaggcgga cagcgataaa
     1861 tttgtagcta tcgtctcccg tttcagctcg cagttcttcc atagctaatt gcgccaaacg
     1921 tcctgccaag gttgagtctt gtcccccaga aatccctagt acaaaggttt ttaggaaggg
     1981 atgttttttc agatatcttt ttaagaaatc aatagaacga cggatttctt cctgggcatc
     2041 aatcactggt ttgacaccca gctcttggat aatcgtttct tgcaaactca ttcttcttct
     2101 ccttcaccaa gggcttcctt gcgcatctta tcaatcaagt ccatcttatc ttgccataca
     2161 tcacgcgcca aatccactgg atagtgctgc ggattgagca cacgcttata ctcatcccac
     2221 aacttgtcaa attccttacg ggcataatcc tgaatgtcag tcaaactagg caagttgtaa
     2281 actaatattc cttctttgaa gatatccacc aagagaggaa cggcatcaaa attacgaacc
     2341 gtcttcttga tgtatgtata ggtcggatgg aacatcttga tttctgtcat gtcgctaata
     2401 tccacaccat cataagtgat atagtcgcct tctgacttgc ctttttcacg actggtaatg
     2461 cgccacacct gcttcttacc tggcgtagaa actttttcag cattattaga cagcttaatc
     2521 gtattgcgca tctgaccagt ttcatcttcg attgcaacaa tcttgtaaac cgccccaaga
     2581 gccggctggt cataggctgt aatcagcttg gtacccacac cccagacatc aatcttggcc
     2641 ttttgcatct tgaggttaag gatggtattt tcatctagat cattagaagc ataaatctta
     2701 gcctctgtaa atccagcctc atccagttgc tgacggactt tcttagaaat gtaggcaata
     2761 tccccagagt caatccgcac acccataaag ttaatctgat cacccagctc acgcgccacc
     2821 tgaatggcag ctggtacacc gatgcgaagg gtgtcatagg tatccacaag aaagacacaa
     2881 tttttgtggg tcgcagcgta agccttgaaa gcttcatagt cattgccata aacctgtacc
     2941 aaggcatggg catgggttcc caaaacagga atgtcaaaga gcttacccgc acgcacgttg
     3001 ctggttccat tggcgccacc aatcacagct gcgcgtgttc cccagatggc cgcatccatt
     3061 tcttgagccc gacgtgtccc aaactccatc aagggttcat cttcgataac cgaacgaata
     3121 cgagctgcct tcgtcgccac caaagtctgg tagttgacga tgttcaaaag agccgtttcg
     3181 accaactgac attgggctag aggtccttcc acctgcacaa tcggttcatt agcaaaaacc
     3241 aaatcccctt cttgggcaga acgaacggtc aactccaact tgaaattgcg aaggtaatcc
     3301 aagaacgccc catgataacc aagcgactcc aaataggcta tatcactatc tgaaaaacgc
     3361 aagtcttcaa gatagttcac aattctttcc aaacctgcaa aaaccgcata gccgttctta
     3421 aaaggctgtt ggcggaaata cacctcaaag accgccttct tattgtgaat cccttggtca
     3481 aagtaaacct gcatcatgtt gatctggtac aagtccgtgt gcaatgtcaa actatcatct
     3541 ggatacatac ttttcctact tccttagcta gaaacccatg aaaattttca agaactttca
     3601 tgtattccaa taaattagta ctattatatc acattttagc tggattgaga aaagagtaac
     3661 aagctattct ccactctcca attcatccat atcttgttca aattttttct gagcccattc
     3721 gccatagctc ttaagaccaa gattgccaat aaagatccac ggaaggtaaa tgatataagt
     3781 aatgacccaa gcagacaggt atttaaaatt caaaggattg tgctgataaa tttctatgtt
     3841 gaattgataa ttctgcaaca tcaaaagagc cgtaatagcc aaggttagga aaaaacaacc
     3901 caaaatcgta aaatgaaaac gaccatagta ggtcactccc agataacggg cacgattgaa
     3961 aaagtaaaat gtccctatga tgataacgat tagcagcata ttagaattaa aaaggcttgg
     4021 tgctaatact gaaatgatat aagataggag cgacaaagca atgcagatat agaaactttc
     4081 agagcccgct ttattgaaca gttgttcttc tctttcgtct agtaattgag aataataaaa
     4141 tctatttttc atcttcttcc tcccaaaata gttggtctag ggttttccct aaacatctgc
     4201 aaatagactg gcagagcgag agactgggat tgtattttcc cgcctctatt aaaccaatag
     4261 tctggcgtgt caccccgaca gcctctgcca gttgaccttg tgttaaatca cgctctaccc
     4321 gagctaattt taattttaaa tttttagcca ccttcgtcct ccttatagtt ttaatactca
     4381 tctactctta aaaaatccaa aaccaacaca agctatcagc tatttattat aaccccatta
     4441 atttgcactc cagtattatc ttcagccgaa aatcagtatt ttaggttgtt tgaaataaac
     4501 ttcctagttt actcttttat ttttatttag tattagcttt agtaagatct ttta
//
LOCUS       DAVHEW010000117         4389 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVHEW010000117 DAVHEW010000000
VERSION     DAVHEW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.117"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(293..2551)
                     /gene="glgP"
                     /locus_tag="VVL53_001937"
     CDS             complement(293..2551)
                     /gene="glgP"
                     /locus_tag="VVL53_001937"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU4184048.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAVVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERFYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2577..4094)
                     /gene="malQ"
                     /locus_tag="VVL53_001938"
     CDS             complement(2577..4094)
                     /gene="malQ"
                     /locus_tag="VVL53_001938"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU4184049.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
BASE COUNT         1279 a          894 c          894 g         1322 t
ORIGIN      
        1 ggtaaccaac tacgaaaaaa tataaggctg aggacagtaa aaaccagcat ttaaagcctt
       61 cactgtatct aggatttccc tatttttaga caagattgac ccaaatctac atcattaaaa
      121 aagcgaattt caatcgaaac tcgctttttt acaaaatgta ttagtataaa tttgtatctt
      181 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaagcactg
      241 ctttgaggtt gcagataaag ctgacgtggt ttgaagagat tttcgaagag tattagttca
      301 agtgccagat gtcttcgtta tactgagcga ttgtacggtc agatgagaag aatcctgctt
      361 tagaaatgtt aacgatgact ttatccaacc atgcgtcacg gtcttcgtag tcagcaagca
      421 tttgctcttt gactttgatg tagtcttcca aatcaagaag agtcatgaac cagtctttgt
      481 tgatcaattc attgtaaaaa cgttccaagc gctctttgtt tccagctgca agaactgcat
      541 cactaacgat gaagtcaacc aatggtttga tagcttcacg agcgtagaat tcgcttgatt
      601 tgtaagctgc ttttgcgtaa aggtcgataa cagtttctga atcttcaccg aagatgtaga
      661 tgttttcttc tccaaccaac tcagcgattt ccacgttagc accgtccata gtaccaagtg
      721 tcaaagctcc gttcaacatg aatttcatgt taccagtacc tgaagcttct ttagaagcaa
      781 gtgagatttg ttctgagata tcacatgctg ggataaggaa acttgctgca gtaacgttgt
      841 agttttcaac cataactact tgcaagtgtg gagctactgc tggatcgtta gcaataactt
      901 ctgacatgca aaggattaaa tggataatgt cttgagcgat tgtgtaggct ggagctgctt
      961 taccaccaaa gaagattgtg attggacgag cagggatgtt accagctttg atgtcaaggt
     1021 atttgtggat cacgtacaaa gcgttcattt gttggcgttt gtactcgtga agacgtttga
     1081 tttggatatc aaagatagaa tttggattga tttccacacc ttggtgttct ttcaagtgac
     1141 gagccaattt acgtttgttg tgagccttga tgctttccaa tttttctttg acaactgctt
     1201 tgtcttcata agacaaaagt ttttcaagct catctgcttc atggtgccaa ccatctccaa
     1261 gaatctcatc caagtagtga gacaatcttg ggttagcatg cataagccaa cgacggaaag
     1321 tgataccgtt tgttttgttg ttgaactttt ctgggtaaag gtcgtagaag gctttcaact
     1381 cagaattttt caagatttca gtatggagtg ctgcaacccc gttaacactg tatccgtagt
     1441 ggatatccat gtgagccatg tgaacacgtc cgctctcatc gatgatttga acagctggat
     1501 ctttgtactc tgccttcaca cgacggtcca attcttcgat gattggtacc aagtgaggaa
     1561 ccacttcttg caagaattca agaggccatt tttcaagcgc ttcagcaagg attgtgtggt
     1621 tagtgtaggc agtcatgcta cgaacaattg agattgcttc gtcaagatcg ataccacgtg
     1681 cagtcaaaag acgaatcaat tcaggaatca ccattgatgg gtgagtatcg ttgatttgga
     1741 caactgcgta gtcagcaagg tcatgcaagt tgcttccttt ttcgattgct tcgtcgatga
     1801 tcaattgcgc accgtttgaa accatgaagt attgttggaa gatacggagc aattcacctt
     1861 gacggtcact atcatctggg taaaggaaga gagttaagtt gcgagcgata tctgtcttgt
     1921 caaagttgat accatcttta ataatagaag aatcaactga atccaagtca aacaaacgca
     1981 agcggttttt agtcgctgtt tcataaccag taacatcaat atcgtaaaga gttgatgtca
     2041 aagtaaagtc tgcaaatggt acttggtagc tacggcttga gcgaaccaac cagttttgct
     2101 ctgtcaacca tgcatttgga attgtttctt gttggttgtt tttaagaact tgttggaaaa
     2161 gaccaaagtg gtagttaaga ccaacaccgt caccattcaa accaagagta gcaattgagt
     2221 cgataaagca ggcagccaaa cgtcccaaac caccattacc aagagatggt tccaattcaa
     2281 cttcttcaac ttcgatcaag tctttacctg cagctgcaag ttctttttta acatcgtcgt
     2341 aaagaccaag gttaatcaag ttgtttgaca agagtttacc aatcaagaac tcagctgaga
     2401 tgtagtaaac ttttttctta ccagtgttga ctggtttttg gctgcttgca agcttgctgt
     2461 agttaagaag agcaaggtaa agctcttcat tgctacattc tgcaatggtt ttattgtaac
     2521 gattttgtac aaattcttgt agtgataaca tgtttagttg tctcctgatt attgtcttat
     2581 ttctttaaat ctaccaaatt ttcattaatt cggcgataaa ttgttgtcaa gtcaagcaaa
     2641 ccttcctcga cagctggtgt caattgatct tcagtcatac gccaagacca gtttccacca
     2701 agggtagatg ggaagttcat acgagctgcc tcatccaatt ctagtaaatc ttgcatagtt
     2761 gcaattgcca taaagctaac tgatgaaaat actgtacgaa gcatagcgtg taccactgtt
     2821 tcgtattctt tacggttcgt gtaacgagcc atgtactcac gagtcgcatc atcaatctca
     2881 ttacggtacc aaccaagaac cgtattgtta tcgtgtgttc ctgtgtacat aactgagtta
     2941 gcaggtgcca agtgtgggct atcaatgctt tcgtcttctg ggttgaaggc aaattgaaga
     3001 atcttcattc ctgggaagcc agtacgttca cgcaattcga tcacttcatc tgtcatgaag
     3061 ccaaggtctt ctgcgatgat gtttagctca ccaagttctt ccttaacggc tgcaaaaagc
     3121 ttgtaacctg gacctttcac ccactcacca ggtgctgctg tatcggaacc agcagggatt
     3181 tcccagtaag attcgaagcc acggaagtgg tcgatacgaa cgatatcgta gattttgaag
     3241 ctttcacgca agcgttcaat ccaccatttg tagccgtctt tgtccattgc ttcccagtca
     3301 tagattggat taccccaaag ctgaccagtt acagaaaact catctggtgg gcatcctgcg
     3361 atacaagtag ccttaccatt gacatctgtt ttgaagagat gtggatttgc ccacatatca
     3421 cttgaatctt ccgctacgta gattggcatg tccccaacga tttcgatgtg gttgtcgtta
     3481 gcgtaagctt tcaatttcaa ccattgttgg aagaagaagt attgagtcac acggtggtaa
     3541 accaacttgt ctgccaattg ctcacgatag ctttcaagtg ctgaagcttt acgagcacga
     3601 gcatctgcat ctggccattc agtccaagca agattgtcaa aatactcttt gatagccata
     3661 tactcagcaa agagctcaag ccatgattgg ttgtcttgag caaatttctc aaaatcttta
     3721 acatctccga cttcaaagaa acgtttcacc gctttttcta aaagaggacg acgtgcatag
     3781 tagattttag catagtcaac ttcagacgca tcgctaccaa agtcaactcc ttcaaggtca
     3841 cttgcttcca acaaaccttg ctccaccaag atatctaaat cgataaaatg agtgtttcct
     3901 gcgaaggctg agaaagattg gtaaggagaa tccccgtaac tagttgctcc taatggaagg
     3961 atttgccagt aacgttgttt tgtacggacc aagaaatcaa cgaagtcgta agcactttga
     4021 ccaaatgatc cgattccgta agctcctgga agagaagaga tgtgcatcaa cacaccactt
     4081 tgacgttttt tcataatagc acctcgtgtg ttaaaataat ggaacgttgc gtatttttca
     4141 gacgcaaacg tttgcgttac ttataagtat actccttttc aagcataatt gcaagcgttt
     4201 ttaaaaaact tttttgatta tttttaagga gactaactca ttttagcaaa gataacttaa
     4261 aactatctaa tcctaaagtt tgactctcac gagacagtat acaaagtaca aaacctcctt
     4321 agggagcttg aatttatatg caacaaagca caaacgtcta tagaaaatag atatagatta
     4381 gaaatagga
//
LOCUS       DAVHEW010000118         4351 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVHEW010000118 DAVHEW010000000
VERSION     DAVHEW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.118"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..303
                     /locus_tag="VVL53_001939"
     CDS             <1..303
                     /locus_tag="VVL53_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU4184050.1"
                     /translation="QEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLY
                     KTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
     gene            293..613
                     /locus_tag="VVL53_001940"
     CDS             293..613
                     /locus_tag="VVL53_001940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU4184051.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            676..2451
                     /locus_tag="VVL53_001941"
     CDS             676..2451
                     /locus_tag="VVL53_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU4184052.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            2452..3837
                     /locus_tag="VVL53_001942"
     CDS             2452..3837
                     /locus_tag="VVL53_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU4184053.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3860..>4351
                     /locus_tag="VVL53_001943"
     CDS             3860..>4351
                     /locus_tag="VVL53_001943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU4184054.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRR"
BASE COUNT         1421 a          641 c          935 g         1354 t
ORIGIN      
        1 caagaaatat ttgtgtggtt caataaaata aatccaagct tagattcaat cttttcaact
       61 tatgaattga agatgcagga cgcaacaatt tcatcttctg agttagaatt tttatgtgat
      121 ttactattgt ataaaacttt agatcaagga aggtacaatg tagaggggcc gttagttctt
      181 gctagatatt tattgggatg tgagtttgaa gtaaagaatc tcagaatgat catatcagct
      241 cttcaaaata caattccctt tgaatcaata aaagaaagga tacgcccaca ttatggaagc
      301 taataagtat aaaattggca taattggtag ccgtgatatt attttaccat ttagcatgat
      361 tgggtttgat atatttcctg cctaccaaga acaagaagct ataaatacac taagaaaatt
      421 agctcaatct gattatggtg tcatttatat cactgaagac attgcttcaa tgatattaga
      481 tacaattcgc cattatgatt cccaagttgt gcctgctatt attttattac cgactcataa
      541 acaaggttta aatttaggat taaaacgtat agaggataat gtagagaaag cagtaggaca
      601 caatatttta taataatgta caaaattgtc tgtaatatta ttctataatt tttggactta
      661 gtaaggagaa taactttgac tcaagggaag attataaaag tatcgggacc tctagttatt
      721 gcatcaggta tgcaggaggc taatattcaa gatatttgcc gtgtaggtaa gctagggtta
      781 atcggtgaaa ttattgaaat gagaagagat caggcatcta tccaagtcta tgaagaaaca
      841 tctggtcttg gtccgggaga acctgttgtt acaactggag aacctctctc ggttgaatta
      901 gggccaggat tgatttctca aatgtttgat ggcatacaac gcccattaga tcgatttaaa
      961 ttggctactc ataatgattt tctagttcgt ggggtagaag ttccaagttt ggatagagat
     1021 attaagtggc attttgattc cactatagca attggtcaaa aagtgagtac gggtgatatt
     1081 cttggaactg tcaaggaaac cgaggtagtt aatcataaaa ttatggttcc ttatggagta
     1141 tctggagaag tcgtttctat tgcatctggc gattttacaa ttgatgaagt tgtatatgaa
     1201 ataaaaaaat tggacggtag tttctataaa ggaacgctta tgcaaaaatg gcctgtccgc
     1261 aaggcgcgtc ctgtttctaa acgtttaatt ccagaagaac cattaatcac aggtcaacga
     1321 gttattgatg cattctttcc agtaaccaaa gggggagctg cagcagttcc tggaccgttt
     1381 ggagcaggaa agacagttgt acaacaccaa gtagctaaat ttgccaatgt tgatattgtt
     1441 atttatgtcg gttgtggaga acgtggaaat gaaatgacgg atgtactgaa tgagtttcct
     1501 gagttgattg accctaatac cggacaatca attatgcaac ggacagttct gattgctaat
     1561 acttcaaata tgcctgttgc tgctcgtgag gcttcaattt atacaggaat taccatggct
     1621 gagtattttc gtgatatggg ctactctgtc gccattatgg ctgattcaac ttcacgttgg
     1681 gcagaagcgc tacgtgaaat gtcaggacgt ctagaagaaa tgcctggtga tgagggttat
     1741 cctgcttatc tgggaagtcg tatcgctgaa tattatgaaa gagcaggacg ttctcaggtt
     1801 ctagggcttc cagaacgtga aggaacgatt actgctattg gagctgtatc gccacctggt
     1861 ggagatattt cagaaccagt tactcaaaac actttacgga ttgtgaaagt tttttggggg
     1921 cttgatgctc cgttggcaca gcgacgtcat tttcctgcaa ttaactggct tacatcttat
     1981 tcactatata aagacagtgt gggcacttat atagatggta aagagaagac agattggaat
     2041 agtaaaataa ctcgtgcgat gaactactta caacgggaat ctagtttaga ggaaattgtt
     2101 cgtcttgttg gaattgattc tctgtctgat aatgaacgac taacgatgga aattgctaaa
     2161 caaattcgag aagattattt gcaacagaac gcttttgatt cggtagatac attcacttcg
     2221 tttgcaaaac aagaagcaat gctaagtaat attctcactt ttgctgatca ggcaaatcat
     2281 gctttagagt tgggttctta ctttacagag attatggaag gtaccgtggc agttcgagac
     2341 cgtatggcga gaagtaaata tgtttcagaa gatagattag atgaaatcaa aattatatca
     2401 aatgagatta cacatcaaat tcatttgata ttagaaacag gaggtctata aatgagtgtt
     2461 ataaaagaat acagaactgc tagtgaagtt gttgggcctc ttatgattgt tgaacaagta
     2521 aataatgtgt cttacaatga gttagttgaa attcaacttc ataatggaga aattcgtcgt
     2581 ggacaagttt tagagatcca cgaagataaa gcaatggttc agctttttga aggatctagt
     2641 ggaataaatt tagaaaagtc taaaattcgt tttgctggtc atgcattaga attggctgta
     2701 tctgaggata tggttggtcg tatttttaat gggatgggaa aaccaattga tggtggacca
     2761 gatttaattc cagagaaata tttagatatt gatggtcaag ctattaatcc tgtatctaga
     2821 gattatccag atgaatttat tcagacaggg atctcctcta ttgatcattt gaatactctt
     2881 gtacgtggtc aaaaattacc agtattttca ggttcgggct tacctcataa tgaattagct
     2941 gctcagatag caagacaagc gactgtttta aattctgatg aaaattttgc ggttgtattt
     3001 gcagcaatgg gtattacttt tgaagaagct gagtttttta tggaagaact cagaaaaaca
     3061 ggagcgatcg atcgttcggt tttatttatg aacttggcaa atgatcctgc aattgagcgt
     3121 attgcaactc cccgcattgc tttaactgcg gcagagtatc tagcttttga aaaagatatg
     3181 cacgttctag ttatcatgac ggatatgact aactattgtg aagcgttacg tgaagtctcg
     3241 gcagctcgcc gtgaagttcc agggagacga ggctatccgg gatatttata tacaaattta
     3301 tcaactctat acgaaagggc tggtcgctta gttggtaaaa aaggttcggt gacacagatt
     3361 cctattttaa caatgccaga agatgacata acacatccaa ttcctgattt aactggatac
     3421 attactgaag ggcaaattat tttgtcgcat gagttgtata atcaaggtta tcgtccacca
     3481 atcaatgttt taccttctct ctctcgatta aaagataagg gatctggaga aggtaaaact
     3541 cgtggagatc atgctccaac tatgaatcaa ctgtttgcag cctatgccca agggaaaaag
     3601 gttgaagagt tagcagtagt attaggagaa tcggctttat ctgatgtaga taaattgtat
     3661 gtgaggttta caaagcgttt tgaagaagag tacataaacc aaggatttta taaaaatcga
     3721 aatatagaag atacgttgaa tcttgggtgg gaattactat caattcttcc tagaacagag
     3781 ttaaaacgta tcaaagatga tttgcttgat aaatacttac ctttggtaga agtttaatcc
     3841 ggaaatggag tgattatcta tggtacgttt gaatgtaaaa ccaactcgta tggaattgaa
     3901 taacttaaag gaacgtttga caacagctga acgtggacat aagttattaa aggataaaag
     3961 agatgaattg atgaggcgat ttatttcttt gattcgtgag aataatcaac ttcggaaaga
     4021 agtggaaagt tatctaattg ataatctaaa atcctttgca gttgctaaat cattaaagaa
     4081 ttctcaaatg gtggaggaat tattttcaat tccatcgaaa gaaattgaat tatttgttga
     4141 gaaagaaaat atcatgagtg taacagttcc tagaatgcat atgaatatta cttctcaaaa
     4201 tgagaacagt gaatacagct atttatcttc taatagtgaa atggatgatg tatttgctac
     4261 aatgaatagt ttaatttata aattactaag actggcagaa gttgaaaaaa cgtgtcagtt
     4321 aatggctgat gaaatagaaa aaacacgtag a
//
LOCUS       DAVHEW010000119         4325 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVHEW010000119 DAVHEW010000000
VERSION     DAVHEW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.119"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            2..2182
                     /locus_tag="VVL53_001944"
     CDS             2..2182
                     /locus_tag="VVL53_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU4184055.1"
                     /translation="MVLSAFVANSVAAQETETSEVSTPKLVQPVAPTTPISEVQPTSD
                     NSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVTKELKDKFTSGDFTV
                     VIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEARDTSSNKNNLVSRP
                     ASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNFLNIKDIKGIDYYML
                     GGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHLIYTANDTTGSNYFR
                     IPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTWTKPKLTLAFDDFAP
                     VPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPAGVSFREATRKDSGY
                     KQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDKNFGIKQNGNYLTVE
                     QYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISSNDHGESWSAPTLLP
                     PIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDNGASWKVKVVPLPSS
                     WSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYLKFVSNPSYGTQLSI
                     INYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRYHHDVDYSNYGYSYS
                     TLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN"
     gene            2222..3331
                     /locus_tag="VVL53_001945"
     CDS             2222..3331
                     /locus_tag="VVL53_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4184056.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            3488..>4325
                     /locus_tag="VVL53_001946"
     CDS             3488..>4325
                     /locus_tag="VVL53_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4184057.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNE"
BASE COUNT         1480 a          638 c          900 g         1307 t
ORIGIN      
        1 agtggtatta tcagcatttg ttgctaactc agttgcagct caggagactg aaacttctga
       61 agtatcaaca ccaaagttgg tgcaacctgt tgcaccaacg actccgattt cggaagtaca
      121 acctacatcg gataactctt cggaagttac tgtacaacct cgaacagttg aaactactgt
      181 taaggatcca tcttctacag cggaagaaac tcctgtctta gaaaaaaata atgttacttt
      241 aacagggggc ggagaaaatg ttactaaaga gttaaaggat aaatttacta gcggtgactt
      301 tactgtagtg attaagtaca atcagtcaag tgagaaaggc ttacaagctc tgtttggaat
      361 atctaattcc aaacccggtc aacaaaatag ttatgtagat gtgttcctta gagacaatgg
      421 tgagttgggg atggaagcgc gtgatacttc ttccaataaa aataacctag tatccagacc
      481 tgcttcagtt tggggtaagt acaaacaaga ggctgtgact aacactgttg cagtagtagc
      541 agattcagtc aaaaaaacat attctttata cgcaaatggt acaaaagtag tagaaaagaa
      601 agtggataat ttcctaaaca tcaaggatat taaaggtatt gattactata tgcttggggg
      661 agtgaaacgt gcaggaaaaa cggcgtttgg ttttaacgga acactagaaa atatcaaatt
      721 ctttaatagt gcattggatg aagaaactgt taaaaagatg acaacaaacg ctgttactgg
      781 acatttaatt tatacggcta atgatacaac aggttctaac tatttccgta ttccagttct
      841 gtatactttt agcaatggtc gggtattttc aagcattgac gctcgttacg gtggaactca
      901 tgatttcttg aataaaatta atattgctac aagttatagt gatgataatg gtaagacatg
      961 gactaaacca aaattaacat tggcattcga tgattttgcg ccagtaccat tagaatggcc
     1021 tcgtgaagtt ggtggacgtg acttacaaat cagcggtggt gcaacctata ttgactctgt
     1081 tattgttgaa aaaaagaaca aacaagtact catgtttgct gatgtgatgc ctgctggagt
     1141 aagttttaga gaagcaacta gaaaagattc aggttataaa caaattgatg gtaattatta
     1201 ccttaaatta aggaaacaag gtgatactga ttacaattat actattcgtg agaatggtac
     1261 tgtatacgac gatcgtacca acagaccaac tgaattttca gtagataaaa atttcggtat
     1321 taaacaaaat ggtaattatt tgacggtaga gcaatattcg gtttcatttg aaaataataa
     1381 aaagactgaa tatcgtaatg ggactaaagt ccatatgaat atcttctaca aagatgcatt
     1441 gtttaaagta gtgccaacga actatattgc atatatttct agcaatgatc atggcgaatc
     1501 ttggtctgca ccaactttat tacctcctat aatgggactt aatcggaatg cgccatattt
     1561 aggtcctgga cgtggaatca ttgaaagctc aactggacgt attcttattc cgtcttacac
     1621 tggtaaagag tctgcgttca tttatagtga cgataatgga gcatcttgga aagttaaagt
     1681 agtgccactt ccttctagtt ggtcagcaga agcacaattt gtagaattga gtccaggagt
     1741 aattcaagca tatatgcgta caaataatgg taaaattgca tatttaacaa gtaaagacgc
     1801 aggtactact tggagtgcac cggaatattt gaaatttgtt tcaaatccaa gttatggaac
     1861 acaattatca atcatcaatt atagccaatt gattgatggt aaaaaggctg tcattttaag
     1921 tactccaaac tccacaaatg gtcgtaaaca cggacaaatt tggattggtc taattaatga
     1981 tgataataca attgattggc gttatcatca cgacgttgat tatagtaact atggatactc
     2041 atattcaaca ttgacagagt taccaaatca tgaaattgga ttgatgtttg aaaaatttga
     2101 ttcatggtct cgtaatgaac ttcatatgaa aaatgttgta ccatatataa catttaagat
     2161 tgaagatctg aaaaagaatt aaagctgaaa tttgaaaata tataaaaaga ggataaaaat
     2221 tatggtaaat tacggtattg ttggagctgg atattttgga gctgatttag ctcgctcaat
     2281 gaacaaaatt gaagatgcaa aagtggttgc ggtatttgac ccaaatcatg gagaagaagt
     2341 tgctcaagag ttgggatcag atgtttgtgc aagtttagat gaacttgtag cacgtgaaga
     2401 tattgattgt gtgatcgtag cttcacctag ctaccttcac cgtgaaccag ttgtgaaagc
     2461 tgctcaacat ggcaaacacg tattttgtga aaagccaatt gcattgtctt atgaagattg
     2521 taaagccatg gttgacgcat gtaaagaaaa taatgtcatc tttatggctg gtcacatcat
     2581 gaacttcttt aacggtgtac accatgctaa agaattgatt actcaaggta aaatcggtaa
     2641 agttctttat tgccatgctg ctcgtacagg ttgggaagaa caacaaccaa ctgtatcatg
     2701 gaagaaactt cgttctcaat ctggaggaca tttgtaccac catattcatg aattagattg
     2761 cattcagttt atcatgggag gacttcctga aaaagcgaca atggtaggag gcaatgtata
     2821 tcataaaggt gaaaactttg gtgatgaaga tgatatgctc attgtaaact tagaatactc
     2881 tgatgatcgt tatgctgttt tggaatatgg taatgctttc cgttggggtg aacactacgt
     2941 cttgattcaa ggaactgaag gagctatcaa acttgacttg ttcaatactg gcggtactct
     3001 tcgtgttaaa ggtgaaggag aatcacactt cttagttcat gaaactcaag aggaagatga
     3061 tgatcgtaca gctatctata ccggtcgtgg tatggatgga gcaattgcgt acggtaaacc
     3121 aggagtacgt tgcccattat ggttgcaaac atgtattgat aaagaaatgg aatatctaca
     3181 tgacatcatt aaaggtggag aaattacaga agaatttgaa aaacttctca atggtgtagc
     3241 tgctttagaa tcaatcgcta ccgctgatgc atgtacttta tcagttaaag aagatcgaaa
     3301 agtaagtctt tcagaaatca caaatgctta acttttgtaa aacagaatag taaattcttg
     3361 tcattatata atttctaaag ttctgtgata caactcattg aataaagaaa tagagatggg
     3421 actgggataa tgcccagtcc cattttttat caaaaagtaa tgagatcaaa aatgtgggag
     3481 tgttgaaatg aagattatag gtatcgatat tggcggaaca acaattaagg cagatttata
     3541 cgatgagttt ggaacgagtt tgaatcattt caaagagata gaaacaatta ttgactatga
     3601 tttgggaacg aatcagatat taaatcaggt ctgtgattta attggtgagt atactttaaa
     3661 tcattcaatt gatggtgttg ggatttccac tgctggagtt gttaatgcta atactggaga
     3721 aatcatctat gcaggctata caataccagg gtatatcgga gtaaacttta ctgccgaaat
     3781 agaaaaacgt tttgggttgt atacttttgt tgaaaatgat gttaattgtg ctgcattagg
     3841 tgaattgtgg aagggacaag ccaaagataa gaaaaatgta gtaatggtta ctattggaac
     3901 aggtatagga ggcagtatta ttgtcaacgg acaaattgtt aacggattta actatactgc
     3961 tggtgaagta ggttatattc ctgtaggtaa ttcggattgg caaagtaaag cctcaacaac
     4021 cgcattgatt catttatatc aaaaaaagag cttgaaaact aatcaaactg gacgtacttt
     4081 cttcactgat ttaagatctg gagataaagt tgctgaagaa acttttgaaa tttttgtaga
     4141 aaatctaaca aaaggtttat taacgatttc ttatctactt aatccagaaa ttctcatatt
     4201 aggaggtggg attctggata gtaaggatat tttgttacct gaaattcaaa gttctttagc
     4261 taaaaatgca atggataata ggtttttacc taaaaatctt gtggcagcta cattaggaaa
     4321 tgaag
//
LOCUS       DAVHEW010000120         4307 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVHEW010000120 DAVHEW010000000
VERSION     DAVHEW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.120"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..651
                     /locus_tag="VVL53_001947"
                     /pseudo
     CDS             1..651
                     /locus_tag="VVL53_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1483..1839
                     /locus_tag="VVL53_001948"
     CDS             1483..1839
                     /locus_tag="VVL53_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184058.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            1844..2209
                     /locus_tag="VVL53_001949"
     CDS             1844..2209
                     /locus_tag="VVL53_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU4184059.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            2196..4019
                     /locus_tag="VVL53_001950"
     CDS             2196..4019
                     /locus_tag="VVL53_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU4184060.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            4133..>4307
                     /locus_tag="VVL53_001951"
     CDS             4133..>4307
                     /locus_tag="VVL53_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4184061.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVS"
BASE COUNT         1515 a          672 c          902 g         1218 t
ORIGIN      
        1 taatcaatgg ttaagtgaaa atattataag tgagaaagat ggaagttatc aacattcact
       61 gactataatg aaggatttca ataatcaaaa aatagttaac tatattgctc atttcaatag
      121 ttatgacatt ttgaaaaaag gttctcactt atcaggtgca tataaacgaa aaattttttc
      181 taaagaaaat acacgatttt ggagtttaat attaactcga aagaatcttc gcaacaactt
      241 gatattctct ataaagaagt tggtgtatta agtttgggac aaaaagttgt agccatgctt
      301 gattttttat tagcatatag tgattattct aaagacttca gaccattgat tattgatcag
      361 cctgaagaca atctagacaa tcgttatatt tacaggcatt tagttcagca gtttagagat
      421 gtgaaagctc aacgtcaaat tattttagca acacataatg ctacaattgt aacaaattct
      481 atgacagatc aagttgttat tatggagtca gatggagtta acggatggat tgaatcacag
      541 ggatatgtta gtgaaaaata tataaaaaat catatcatca atcaattaga gggaggaaaa
      601 gattccttca agcataaaat gtctatatat gagacggctt tatcagagta gagtcagaaa
      661 aagtaggtta gaaatttagc ctactttttt ctttgtccga caggcatagt gtacatctga
      721 ggtccaagtc ctctgtggat atttgctgca gatgaaacca atagcgactc ctaagcctga
      781 atatcgtgag gtagggggga taggaaggaa ttagcgaaat caaggttcta caaacagaat
      841 cgtgacttga agccatatat agcggatgag gaactctaaa atccaaatag gtgtcgtaac
      901 ctatatacgt aaattacgag agtaaactag gaaagatgta cggcttattc cgtgagcgtt
      961 taggacgtag tacaacgaat catgggagtc agctgaacac atagtattga agaaatttct
     1021 gtaatggaaa tggagcgaag aagtgaacaa ttaaatgaat acctctctaa ttaaatttgt
     1081 caattctaat tcctggtatg aaaagacagt gacctgaaaa tgtaaacgat gggagctgat
     1141 cataaatata ggacggtaca tgcagtggtg ttagagatta gtccttactt gatttgtgat
     1201 aacttcccca aatttcttct gctatacttt tctcaacttt taaaaatcca actaagaatt
     1261 ttacctgggg gtttgggggc ggagccacta agttatctta tcgttagctg tcaaaactgg
     1321 taggttttga taggctggcg atatgatttt tgggatattg tggacacaat atctgagctc
     1381 gcaaagcctt acaagaatga aaatcagttg ttggaaaagt gtactgacat tgtatggtag
     1441 ctcacattgt cagtacaagt attttggaaa ggaagtagca gtatgaaacg agatgtgcgt
     1501 gatattcgga aacaatttcg tttaacagaa gcagaagaaa agcaaattct agctttgatg
     1561 agagagcggg gagagactaa tttctctgat tttcttcgta aaagtttact ttcctctgat
     1621 ttacaaaaac agatggagac atggtttgcc ctctggcaat cccaaaaact agaacaaatc
     1681 agtcgtgacg ttcatgaagt tttaatcttg gcacagtcag aacgtcaagt cacccaagag
     1741 catgtatcta ttctcttaac gtgcgtgcag gaattgattc aagaggttgc aaacaccata
     1801 cccctcagta aagaatttcg tgagaagtac atgaggtaag cacatggaac atcgttaccg
     1861 aaccaatctc aagaaagtgt ttttgtctga tagtgagttg aaccaactaa atataaatat
     1921 cgatcaaagt ggttgtaaat ccttttctga atatgcgaga cgaactctac tcgatcctgg
     1981 tatgaatttt atcacgattg acacaaacgg ttaccaagat ttagtgtttg agttaaagag
     2041 gattggcaat aatatcaacc agattgctcg aagtgttaat caatctcagt taatttctgg
     2101 tgaagaattg caggagttga aaaaaggaat tggtgaattg ataaaagaag ttgataagga
     2161 atttaatctg caagcgcaga agctaaagga gttccatggt catcactaaa cactttgcca
     2221 ttcacggaaa gagttaccgc agaaagctta tcaagtacat tctcaatcct gagaaaacca
     2281 ataatcttgc cttggtgtcg gactatggca tgaagaattt tctggacttt cctagctatg
     2341 aggaaatggt gcagatgtat catgaaaatt tcatcagcaa cgatacgctt tacgattttc
     2401 gccacgacag gatggaagaa aatcaacgaa aaatacacgc tcaccacatc attcagtctt
     2461 tctcgccaga ggatcatctc actcctgaac aaatcaatcg gataggttat gagactgtga
     2521 aggaattaac tggtggcaaa tttcgtttta tcgttgcgac ccatgttgat aaagaccacc
     2581 tgcacaatca catcattatc aattcagtag atagcaattc tgacaaaaag ctcaagtggg
     2641 actacaaggt ggagcgaaat cttcgcatga tttctgaccg tttttctaaa atcgcaggtg
     2701 ctaaaatcat tgagaaccgc tattctcacc agcggtatga agtctatcgt aagactaatc
     2761 acaagtatga actcaagcag cgactctatt ttttgatgga acattctagg gactttgagg
     2821 atttcaaaaa gaatgctccg ctactacatg tggagatgga tttccgtcac aagcatgcca
     2881 ccttttttat tacggactca actatgaaac aggtggtgcg tggcaagcaa ctcaatcgca
     2941 agcagcctta cacagaagaa ttttttaaga actactttgc caaaagagaa atagaaagtc
     3001 tcatggaatt tttattgctg aaagttgaga atatggatga tttacttcag aaagcaaaac
     3061 tttttggact aactatcaat cctaaacaaa agcatgtttc ttttcaattt gcaggagtgg
     3121 aggtaaagga gacagagcta gaccagaaaa atctttatga tgtagagttt ttccaagatt
     3181 attttaaaaa tagaaaagat tggcaagctc cagaaactga ggatttcgtt caactttatc
     3241 aagaagaaaa gttatccaaa gaaaaagaac ttccaagcga tgagaagttc tgggagtcct
     3301 atcaagagtt caagagtaac agagatgccg ttcatgaatt tgaggtggag ttgtcactca
     3361 atcaaattga aaaagtagtg gatgatggaa tttacgtcaa ggtcaagttt ggtattcgtc
     3421 aggagggact tatctttgtg ccgaacatgc agcttgatat ggaagaggat aaggtgaagg
     3481 ttttcatcag ggaaaccagc tcctactatg tctaccacaa agacgctgcc gagaaaaatt
     3541 gttatatgaa aggtcgaacc ttaattagac agttcagcta tgaaaatcaa accattccat
     3601 tacgcagaaa agcgacagtc gatatgatta aagagaagat tgcggaagtg gatgctttga
     3661 ttgaactgga agtagaaaat caatcttatg tcacgattaa agatgagtta gtgcatgaac
     3721 tagcagcgtc tgaattgaga atcaatgagt tgcaagaacg aatgtcaacc ttgaatcaag
     3781 tagcagaata tctactggct tcagttgaaa gtaagcaaga aatgaaatta aatctttcaa
     3841 aactgaatat aactgagaat atcagtgcta atattgttga gaaaaaattg aagagcctgg
     3901 ggaatcaact ggaattggaa aggggcaggt atgaaaagat ggtagtcagg ctagataaat
     3961 ttataaatcg cttaaataca ggactttcaa aaggagatgg gatagatttt caaaaatagg
     4021 aatctagcca ataattatat acaatgtgta tcttttgtaa aatatatttg aaatttagat
     4081 ttaatcatgt tacaattaac attgaaatca aatagaaaag gaagatgcaa acgtgggtac
     4141 actattagca acaagattaa aaaatagacg aaaagaatta aaaatgtctc agcgagaatt
     4201 agctgagggt atctgtaaac aggggcaaat tagtcgatta gagagtggag agttcacccc
     4261 tggagcggat tttttacatg cccttgctaa gaaattaaaa gttagta
//
LOCUS       DAVHEW010000121         4305 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVHEW010000121 DAVHEW010000000
VERSION     DAVHEW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.121"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..279
                     /locus_tag="VVL53_001952"
     CDS             <1..279
                     /locus_tag="VVL53_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU4184062.1"
                     /translation="DYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDEALFRFSIL
                     NANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(446..517)
                     /locus_tag="VVL53_001953"
     tRNA            complement(446..517)
                     /locus_tag="VVL53_001953"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:cct)
     gene            complement(560..907)
                     /gene="rplS"
                     /locus_tag="VVL53_001954"
     CDS             complement(560..907)
                     /gene="rplS"
                     /locus_tag="VVL53_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU4184063.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1026..1355)
                     /gene="crcB"
                     /locus_tag="VVL53_001955"
     CDS             complement(1026..1355)
                     /gene="crcB"
                     /locus_tag="VVL53_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU4184064.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1349..1723)
                     /gene="crcB"
                     /locus_tag="VVL53_001956"
     CDS             complement(1349..1723)
                     /gene="crcB"
                     /locus_tag="VVL53_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU4184065.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1720..1986)
                     /locus_tag="VVL53_001957"
     CDS             complement(1720..1986)
                     /locus_tag="VVL53_001957"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU4184066.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2102..2545)
                     /locus_tag="VVL53_001958"
     CDS             complement(2102..2545)
                     /locus_tag="VVL53_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU4184067.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2649..3584
                     /locus_tag="VVL53_001959"
     CDS             2649..3584
                     /locus_tag="VVL53_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU4184068.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3638..3922
                     /locus_tag="VVL53_001960"
     CDS             3638..3922
                     /locus_tag="VVL53_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU4184069.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3979..4197)
                     /locus_tag="VVL53_001961"
     CDS             complement(3979..4197)
                     /locus_tag="VVL53_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184070.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(4194..>4305)
                     /locus_tag="VVL53_001962"
     CDS             complement(4194..>4305)
                     /locus_tag="VVL53_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU4184071.1"
                     /translation="LEDVMNQLQDVIQGLEKEVIKSIGNRRWTRMLFESR"
BASE COUNT         1355 a          935 c          752 g         1263 t
ORIGIN      
        1 gattatccag tatattatcc cgattcgata cctgactact cactaaaaca tattcaaaca
       61 ttaatggaat cattatctga tgacgattat gattttgctt ttgatgaagc attatttcgc
      121 ttctcaattt tgaatgcaaa tcatttttta tctaaggaag atattgacta tttaagagtt
      181 aatttacctc gttccactgc tgaagaaata aacaattact taaagaaata tgaatgttat
      241 agtcctttaa ataatttaga acttgacgat tttgaataaa ttgactatac aaacatttat
      301 atactcgata tagtctcaat tttatctgat gattgcccaa atttttcaat aataaaacgc
      361 ataatattat ggagacaatc ccctatatta tgcgttcttt taatatcaaa gactttttga
      421 caaacttctt tgatatctaa ttacatgccc cctgcaggaa tcgaacctgc aactactcct
      481 taggagggag ttgttatatc cattgaacta agggagctag ataaaaactc tgctaaatga
      541 gcagagtttt ttagtcgaat taacgacgga tttctttgat acgagctgct ttaccttgaa
      601 gagcacgcaa gtagtacaat ttcgcacgac gtactttacc gtaacgaaca acttcgattt
      661 tttcaacacg tggagtgtgg attgggaaga tacgctcaac acctacaccg ttagagattt
      721 tacgaactgt gtagttttct gagattccag cacctttacg tgcgataaca acaccttcaa
      781 aaatctggat acgttcacgg ttaccttcga caactttcgc atgtacacga acagtgtcac
      841 caggacggaa tgatgggata tctgtacgaa gttgaccttc agtcaagctt tggattaatg
      901 gattcatttt attctcctat ctttgtcaat cttgaggaat cttcctcagc ggataaactg
      961 tatttttgtg cgtccattac acacaagata tagtttacca aatttctaaa aaaaagtaaa
     1021 gaaatttata gcaaaattcc taaaaaaatc gcaaccaaac cacctatgta agtcaaagtc
     1081 aggtaagaat aaaagacctt cttatcactt agcagtcttt gaagttcgtc attcaaggtc
     1141 gaaaaagttg ttaaaccgcc acaaaatcct gttgctagaa tagcatatac ttccttagac
     1201 tctacatgat tgtagaatac tccaattaaa aaacaaccta aaagattagc tatgagcgtt
     1261 ccgagtggca atttagaagc ttgattatag cgagaaaaga aataccgtac gagtgctccc
     1321 aagccacagg cgattgcaag atagacgatt accatttctt cctccccaaa tagtaagcta
     1381 aaagcaggcc tccaccgata ctcaaaagca aatacagtat caaactaaga taacgccctg
     1441 tatcaagcag cttcacagta tcaagcatta ggctggaaaa agttgttaaa cctccgcaaa
     1501 aacccgtccc cagtgctaaa atcaagccct tactagtccc cttatagacc agatagccct
     1561 tgacaagata aatcaagcag aaaattccca gatagttgac aaagagagtg ccccaaggga
     1621 aatctggact ggctggtaac caggtggaaa ctagatatcg gacaagtccg cccaacatag
     1681 cagctagaaa aattcctagc ggataaaatt gttctttttt catttgatat tttgatcctg
     1741 ataatcacgc gaacgtttga gtatgtccga aaaagtcgcg acaatagtct cctgatagcg
     1801 cttgtcctct acacgactcc taaccttttc aaaaataact tcttctctct tagtatctaa
     1861 aatcggttta cctgaagctt tcttataagc aaccaccccc tcaactaaat gcattcgttc
     1921 ttctaacagt ttgacaattt ggtcgtcgat ttgatcgatt tcttgacgaa taatatctaa
     1981 atccatacag tctcctcctt tatttgaatt attgtatcaa aaagccatca attaggctag
     2041 taaaatccca actctcgata agaaaaatgc actgattgat tacatcagcg cacttttatg
     2101 tttatcctac tttagcagcc aattcttctg cgaattgttc caagcgttca atatcttctt
     2161 cctcggcaga aagatcaact ttaacacact ctgaaccttt ttctgctcct gttgacacaa
     2221 agacacggtc aaagtcatca acagccttac agaattcatc gtagaaggta tctcctgagc
     2281 cgaccactcc gtagattttg ccattcaagt tgagatctgc taggtcttcg tagaagtcca
     2341 tcatctcatc tggcaattca ccatcaccat aagtataggt cgcaacgata gcgatgtctg
     2401 cttccaagaa gtctgaagcg tcaacagttg tacattcatc aacatcaaca tccaagccca
     2461 agtcacgtaa tttgtctgct acaatatctg caatttcttc ggtattaccg gtcatactgg
     2521 caaatacaat ttttgctaat gccatatcgt cctcctcaat ttatctttct ccattatatc
     2581 acatcttttt cattttaaaa ataaaaattc ttgtgctaca atgaaatgag aaagaattga
     2641 ggttatttat ggagatttgc caacaaattt tagaaaaaat taaagaatac gacaccatta
     2701 tcattcatcg tcatatgaaa ccagaccctg atgccttggg aagtcaggtg ggattgaaag
     2761 ccttgctgga acatcatttc ccagaaaaaa ccatcaaagc cgtcggtttt gatgaaccaa
     2821 ctcttacttg gatggctgag atggatcttg ttgaagatag agcctaccaa ggcgcacttg
     2881 tcatcgtctg tgatacagct aatactgctc gtatcgatga taagcgctat agtcaaggtg
     2941 attttctcat taagattgac caccatccaa atgatgatgt atacggtgac ctgtcttggg
     3001 tcgatactag ttcaagtagc gctagcgaga tgattaccct atttgcccaa acaacccaac
     3061 tagccttggc agatcgcgat gctgagttgc tctttgcagg aattgtcggt gatacaggtc
     3121 gcttcctcta cccttctacc actgcacgga ctcttcgcct ggctgcttat ttgagagaac
     3181 ataactttga ctttgcggct ctcactcgca aaatggacac tatgagctac aaaattgcta
     3241 aactgcaagg ctacatctac gaccatctgg aagtggatga aaatggtgct gctcgcgtta
     3301 tcctgagtca gaaaatcttg aaacaataca atataaccga tgctgaaact gcggccattg
     3361 taggtgcacc tggacgcatt gacagagtga gtctctgggg aatttttgtc gaacaggctg
     3421 atggccacta ccgagttcgc ttacgcagta aagtccatcc tatcaatgaa attgccaagg
     3481 agcatgatgg tggaggccac cctctagcaa gtggtgctaa ttcctatagc ctagaagaaa
     3541 acgaaatcat ctaccaaaag ttaaaaaact tgcttaaaaa ctgataaaat acttgccaaa
     3601 cttttcagaa tctgatagac tagtatagta acaatctatg gctcgcaaag agaccatggc
     3661 agaaaggaaa tattgcaaaa tgaaaaaaga tatccatcca gaatatcgcc cagttgtctt
     3721 catggacaca actactggtt accaattcct tagcggttca acaaaacgct ctaacgaaac
     3781 agttgagttc gaaggcgaaa cttacccatt gatccgtgtg gaaatttcat cagactcaca
     3841 cccattctac actggacgtc aaaagttcac tcaagcagat ggacgcgtgg atcgtttcaa
     3901 caaaaaatac ggtctcaaat aatgataaga gaacagtttt ggctgttctt ttttgtttct
     3961 tgaaatcaac tgctgttttc atgttccaga ctcatctgta ggttcgattt ccatgctact
     4021 aggcaggaag gaaatagctg tttcaacacg tccataatga gctatactat tgtcacgaac
     4081 cacactttca ttgatggtcc aagtggaatt cattttctta aaagcttctc ggactttttc
     4141 caaatctttg gaggcaatgg cctgctctaa ggtttcaaaa cgaggactta tactcatctg
     4201 ctttcaaaaa gcattctagt ccatctccga ttaccgatgg actttatcac ctccttctcc
     4261 agtccttgta tgacatcttg aagttgattc atgacatctt ccaaa
//
LOCUS       DAVHEW010000122         4243 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVHEW010000122 DAVHEW010000000
VERSION     DAVHEW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.122"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            18..827
                     /locus_tag="VVL53_001963"
     CDS             18..827
                     /locus_tag="VVL53_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU4184072.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            832..1533
                     /locus_tag="VVL53_001964"
     CDS             832..1533
                     /locus_tag="VVL53_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU4184073.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1558..2475
                     /locus_tag="VVL53_001965"
     CDS             1558..2475
                     /locus_tag="VVL53_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU4184074.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2503..4044
                     /locus_tag="VVL53_001966"
     CDS             2503..4044
                     /locus_tag="VVL53_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU4184075.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4063..>4243
                     /locus_tag="VVL53_001967"
     CDS             4063..>4243
                     /locus_tag="VVL53_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU4184076.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPG"
BASE COUNT         1380 a          645 c          826 g         1392 t
ORIGIN      
        1 tcaactaagg agctcatatg ttactgcaaa aagaactaat tccaatgata gaagctaact
       61 taccaaatat ggcatatgct gaaaaagaca ttgctaaatt cttcttaaaa cagcaacctc
      121 tgaatgatta ttcatctaaa gcattgtgcg aataccttaa tgtatccaaa gcaacattga
      181 ctcgatttgc gaaaaaatgt ggttttaaag gttttagaca attcattttc aaataccaag
      241 agatgattca tgagaaagaa aagttggcat tatatacaga ggcaacagaa aaagttttat
      301 ccgactatga ggaaatgttg agaaaaactt acacggttct tgatgaagtt caacttgagc
      361 gtattgctga gatgatagaa actgctgagc gtgtatatct ctacggtaaa ggaagttctg
      421 ttcttgcttt acaagaaatg aagatgagat ttatgcgtct cggagtgatt ggtgaagtat
      481 tatcagacga ggatatgatt ttgtggagta gcttactact taatgaaaat tgccttgtca
      541 ttggagcatc catttcaggt caaactgata ttgtactaga aggtctacaa aaagctgcag
      601 ataaaggcgc taaaacagtt ttaatgacta caagaaaatt tgacgaagaa gattgtttct
      661 ttgatgaact attgttatta gcttcgaccg atcatctctc gtatggcaat cgcatatcac
      721 ctcagtttcc aatactttta attacagact gcttattctc taattatctg gaaagtccag
      781 agagacaata ttattacaat caaactatta tccataagga ggaataataa aatgagccaa
      841 atttggacta aagaaaaatt tataagccaa gttcaaggtg gagtcattgt ttcttgtcaa
      901 gctttacctg gtgaagccct ttataatgaa gaatttagct tgatgccttt tatggctaaa
      961 gcagctttag aggcaggagc agtgggcatt cgcgcaaatt ctgtgcgtga tattaaagca
     1021 attcagaaag tagtagattt accaataatt ggaattatca aaagggatta tccacctcaa
     1081 gaaccatata ttactgctac gatgaaagaa gtagatgaac ttgtagaatg cggaacaaca
     1141 gtcattgcat ttgatgcaac tttaagacca agatatgatg gcttagttgt cagtgaattt
     1201 atcaaaaaaa taaaagaaaa atatccgaat caattgctga tggcggatgt aagtaattta
     1261 gatgaaggtc tctatgcatt taaatcaggc gttgattttg ttggtacaac attatcaggt
     1321 tacacaagta caagtgtaca atcagatgag cctgattttg aactaatgaa aaaattggct
     1381 gattttaata ttccggtaat tgccgaagga aaaattcatt atccagaaca attaaaaaaa
     1441 gcttatagtt taggtgttac cagtgtagtc attggtggag cgattacacg tccaaaagaa
     1501 attgctcagc gatttattaa tgtcatcaaa taaaaaacaa tataaaggag aaatctgatg
     1561 aaagatttaa ctaaatacaa aggcgttatc cctgcatttt atgcttgcta tgatgaaaat
     1621 ggtgaaatta gccaagatcg tgtaaaatct ctggtacaat atttcattga caaaggtgta
     1681 aaaggtatct atgtaaatgg ttcttcaggt gaatgtattt accaaagtgt agaagatcgt
     1741 aaacaaatta ttgaagctgt tatggaagtt gctaaaggta aattaacagt tatcaaccat
     1801 attgcatgta ataacacgaa agatagtatc gaattggcaa aacattcaga aagtgttgga
     1861 gtcgatgcta ttgcagctat cccacctatt tatttcaaat tgccagagta ttcaatcgca
     1921 gcatattgga atgcaatgag tgaagctgcg tcaaatacag attttattat ctataatatt
     1981 ccacaattgg caggggttgc gttgactggt agtttgtatg caacaatgcg tcaaaatcct
     2041 cgtgtgattg gagttaaaaa ttcttctatg cctgtacaag atattcaaat gtttgtagct
     2101 gcaggtggag aagattacat tgtattcaat ggtccagatg aacaatatct tggtggtcgc
     2161 ttgatgggag cagaagctgg tattggtggt acttatggcg ttatgccaga tttgttcttg
     2221 aaattggaaa gtttgattca agaacgagat ttagatacag ctaaaaaact tcaatatgct
     2281 atcaatgaag ttatctataa gatgatatca ggtaaggcaa atatgtatgc tgtagcaaaa
     2341 gaagttttgc gtctaaatga aaaacttgat ttaggttctg ttcgtcaacc tttagaagca
     2401 ttggcagaag gtgacttgga agttgcaaaa caagcagcag aacttattca acaagcacga
     2461 aaagaatttt tataataaat aaaatcaata tggagatatg ttatgggcac aacaggattt
     2521 acaataattg acttaattat cttgattgtt tatttacttg cggtgttggt tgcaggtatc
     2581 tatttctcta aaaaagagat gaaaggaaaa gagttcttta aaggagatgg ttcggttcct
     2641 tggtatgtta cttcggtatc catttttgcc acaatgctca gtccgatttc cttcttggga
     2701 ctcgctggta gctcttatgc aggtagctgg attttatggt ttgctcaatt agggatggta
     2761 gtagctattc cactgacaat tcgttttatc ttacctatct ttgcacggat agacatcgat
     2821 acggcatatg attacttgga taaacgtttt aattctaaag cacttcgtat tatttcagca
     2881 ctcttgttta ttatttatca attgggacgt atgtctatca ttatgtacct cccatcagct
     2941 ggtttatcag tattgacagg aattgacatc aatattttga ttattttgat gggtgtagtt
     3001 gcaattgttt attcttatac tggtggtcta aaatccgtat tatggacaga ctttattcaa
     3061 ggtgtgattc tgattagtgg tgtcgtttta gctttatttg tactgattgc taatattaaa
     3121 ggtggctttg gtgcagtagc agaaacatta gcaaacggga aattccttgc tgcaaatgaa
     3181 aaacttttcg atcctaactt gctttcaaac tccatctttt taattgtgat gggttcaggc
     3241 tttacaatct tgtcttccta tgcttcatct caagatttgg ttcaacgttt tactacaaca
     3301 caaaatatta agaaacttaa taagatgttg ttcacaaacg gtgttttgtc acttgcaact
     3361 gcaacagtct tttacttgat tggtacaggc ttgtacgtat tctatcaagt acaaaatgca
     3421 gatagtgcag ctagcaatat ccctcaagac caaatcttta tgtactttat tgcataccag
     3481 ttaccagtag gtatcacagg tttgatcttg gcagcgattt atgcagcatc tcaatcaact
     3541 atttcaacag gtttgaactc tgttgcaact tcatggacat tggatattca agatgtcatt
     3601 tctaaaaata tgtcagacaa tcgtcgtacg aaaattgcac aattcgtatc tctagcagta
     3661 ggtttattct caattggtgt ttccattgtc atggctcact cagatattaa atctgcatac
     3721 gaatggttca atagtttcat gggacttgta cttggtctac ttggtggtgt atttattctt
     3781 ggatttgttt ctaaaaaagc aaataaacaa ggtgcttatg cagcgctgat tgtatcaacc
     3841 atcgtcatgg tatttattaa atacttcctt cctccaacag ctgttagcta ctgggcatat
     3901 tcattgattt caatctctgt atcagtagtt tcaggttata ttgtatctgt tcttactgga
     3961 aataaagtat ctgcacctaa atatacaacg attcatgata ttacagaaat taaagcggat
     4021 tcaagttggg aagttcgtca ctaatagaaa agagaaaata atatgattat ttctgaacaa
     4081 tctgatttta aacgatatgc ttctgtcaat aaatattttt caaaagtctg tgatttttta
     4141 gaaaatacaa atttgacaga tttagttgat ggaaaaattg atattgacgg ggaaaatgtt
     4201 tttgcaaatt gtatgactta tctagctgat ggagttccag gag
//
LOCUS       DAVHEW010000123         3810 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVHEW010000123 DAVHEW010000000
VERSION     DAVHEW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.123"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..418
                     /locus_tag="VVL53_001968"
     CDS             <1..418
                     /locus_tag="VVL53_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU4184077.1"
                     /translation="KNKVDQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VVL53_001969"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VVL53_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU4184078.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENLKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1430 a          623 c          746 g         1011 t
ORIGIN      
        1 taaaaataag gtagaccaag acagtaaacc tgatgaagat aaggaacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 cacagatgag gctgaaattc ctcaagtaga gaattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa agaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactt aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact
//
LOCUS       DAVHEW010000124         3717 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVHEW010000124 DAVHEW010000000
VERSION     DAVHEW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.124"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            18..236
                     /gene="nrdH"
                     /locus_tag="VVL53_001970"
     CDS             18..236
                     /gene="nrdH"
                     /locus_tag="VVL53_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU4184079.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            318..2477
                     /gene="nrdE"
                     /locus_tag="VVL53_001971"
     CDS             318..2477
                     /gene="nrdE"
                     /locus_tag="VVL53_001971"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU4184080.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2665..3627
                     /gene="nrdF"
                     /locus_tag="VVL53_001972"
     CDS             2665..3627
                     /gene="nrdF"
                     /locus_tag="VVL53_001972"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU4184081.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
BASE COUNT         1147 a          808 c          700 g         1062 t
ORIGIN      
        1 tgaagaggag ttacacaatg gtaaccgttt attctaaaaa caattgtgtc caatgtaaaa
       61 tgaccaagcg tttcttggac agtaataatg tctcttatcg tgaaatcaat cttgatgagc
      121 aacctgagta cgtcgatcaa gttaaagagc tcggttttag cgcagctcct gttatccaaa
      181 caccaactga agtcttttca ggtttccaac caggaaaact gaaacaatta gcataatctt
      241 agtacatcat ccagaagaaa ctgcttttag ggctaactta gaagcctttc ttttgtaatt
      301 agaataaggg aaattttatg ggattaaaac atcttgaaga cgtgacttac ttccgtctta
      361 ataacgaaat taaccgtcct gttaatggac aaatcatgct tcataaagat aaggaagcct
      421 tggatgcttt ctttaaagaa aatgtagttc caaacactat ggtttttgat tcaatcaaag
      481 acaaaatcaa ttacctcatt gaacacaact acatcgaaac agcctttatc aagaaatacc
      541 gtccagagtt cttggaagaa ttggctcaat ttatcaaaga ccaaaacttc caattcaagt
      601 cattcatggc agcttataaa ttttacaatc aatatgcctt aaagactaat gacggtgaat
      661 actatcttga aaatatggaa gaccgtgtct tcttcaacgc cctttatttc gctgatggga
      721 atgaagctgt tgcaatcgat attgccaatg aaatcatcca ccaacgctac caacctgcta
      781 ctccttcctt cttgaatgct ggacgtgctc gtcgtgggga gttggtatct tgtttcctaa
      841 tccaagtgac ggatgatatg aactctatcg gacgttctat caactcagct cttcaacttt
      901 cacgtattgg tggtggtgtg ggaattaccc tcagcaacct tcgtgaagct ggtgcaccta
      961 tcaaaggcta tgaaggagct gcttcaggtg tcgttcctgt tatgaagctt tttgaagaca
     1021 gcttctctta ctccaaccaa ttggggcaac gtcaaggtgc cggtgttgtc tacctcaacg
     1081 tctttcaccc agatatcatc gctttccttt caactaagaa agaaaacgct gatgaaaaag
     1141 tacgtgtcaa gactctatca cttggtgttg tagtacctga taaattctac gaattggctc
     1201 gtaaaaatga agaaatgtac ctcttcagcc cttactctgt agaaaaagaa tacggtgtgc
     1261 cattcaatta catcgacatt actgaaaaat acgatgaatt ggtcgcaaat ccaaatattc
     1321 gcaagacaaa aatcaaggcg cgggatttgg aaactgaaat ctctaaattg caacaagagt
     1381 ctggctatcc ttatgtagtc aacatagata cggctaaccg tgcaaatcct gttgatggaa
     1441 agattatcat gagtaacttg tgttctgaga ttcttcaagt tcaagaacca agcttgatca
     1501 acgatgctca agaattcctt caaatgggaa cagatgtttc atgtaacctg ggttcaacca
     1561 acgtggttaa catgatgact tcacctgatt ttggtcgttc tatccgtgct atggttcgtg
     1621 cccttacttt cgttacagat agttcacata tcgtagctgt accaacgatt gaccatggaa
     1681 atagccaagc ccacaccttt ggtctcggtg ccatgggact gcacagctac cttgcccaac
     1741 aactcattga atatggttca cctgagtctg ttgaatttac aagcatctac tttatgctta
     1801 tgaactactg gaccttggta gagtcaaaca atattgcgcg tgaacgtggt attaccttcc
     1861 acaactttga aaaatcagac tatgctaacg gaagttactt tgacaagtat gtaactggtg
     1921 aatttgttcc aacatcagac cgtgttaaag aactcttcaa aaatgtcttt atccctggtg
     1981 ttgctgattg ggctgaactt cgcgacaagg ttcaagaaga tggtctttac caccaaaatc
     2041 gccttgctgt agcgccaaat ggttctatca gctatatcaa cgacgtttct gcttctatcc
     2101 acccgattac gcaacgtatc gaagaacgcc aagagaagaa aattggtaaa atctactacc
     2161 ctgctgctgg cttgtctaca gaaaccattc cttactacac ttctgcctac gatatggata
     2221 tgcgtaaagt catcgatgtt tacgctgctg cgactgaaca cgtggatcaa ggactttcac
     2281 tcaccctctt catgcgtagt gacattccaa aaggccttta cgaatggaag agggaaaata
     2341 aacaaacgac acgtgacttg tccatccttc gtaactatgc ctttaacaag ggaatcaagt
     2401 ctatctacta cgtccgtacc tttacagatg acggtggaga agtcggcgcc aaccaatgtg
     2461 aaagctgtgt gatttagttt ctagcataga aacactattt ttccctaagc agcactgata
     2521 aaatcaaatc tactatgtca taacataatt tatgtaacat tttaaaagtc gggcttccga
     2581 ctttttgtgc gatactccta tagaattatg gtaaagtaga aataattgtg agttcgcaat
     2641 actaaacaca gaaagagatt ttaaatggaa acttactaca aagccattaa ctggaatgcc
     2701 atcgaagatg tcatcgacaa atcaacttgg gaaaaactga cggagcaatt ttggctcgat
     2761 acacgtattc ccttatcaaa cgacttggat gactggagaa agctatctaa caaagagaaa
     2821 gacttggtcg gaaaagtttt tggtggttta acccttcttg acactatgca atctgaaact
     2881 ggggttcaag cccttcgcgc agacatccgt acaccacatg aggaagctgt cttcaataac
     2941 atccaattta tggaatctgt ccacgctaaa tcttactcat caatcttttc taccttgaat
     3001 actaaggctg agattgaaga aattttcgaa tggaccaata ccaatcctta cctacaaaag
     3061 aaggctgaga ttgtcaacga aatctaccta aacggcagcc cacttgaaaa gaaagttgcc
     3121 agcgtcttcc tcgaaacctt cctcttctac tctggtttct tcactcccct ctactatctc
     3181 ggtaacaaca aacttgccaa cgttgcagaa atcattaaat tgattattcg tgacgagtct
     3241 gttcacggaa cctacattgg ttacaaattc caacttggtt tcaatgaatt acctgaagaa
     3301 gagcaagaaa aactcaaaga atggatgtac gacctactct atactcttta tgaaaatgaa
     3361 gaaggctaca cagagagtct ttatgacggt gttggttgga ctgaagaggt taaaaccttc
     3421 cttcgctaca atgccaataa agctctcatg aacatgggac aagatccact tttcccagat
     3481 tcagctgaag atgtcaaccc aatcgttatg aacggtattt caacaggaac ttccaaccat
     3541 gacttcttct ctcaagtcgg aaatggttac cttcttggcg aagttgaagc catgcaagat
     3601 gatgactaca actacggttt ggactaatac tcttcgaaaa tcaaattcaa accacgtcag
     3661 cttcgccttg ccgtactcaa gtacagcctg cggctagctt cctagtttgc tctttga
//
LOCUS       DAVHEW010000125         3561 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVHEW010000125 DAVHEW010000000
VERSION     DAVHEW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.125"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(78..1493)
                     /locus_tag="VVL53_001973"
     CDS             complement(78..1493)
                     /locus_tag="VVL53_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU4184082.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            complement(1506..3344)
                     /locus_tag="VVL53_001974"
     CDS             complement(1506..3344)
                     /locus_tag="VVL53_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU4184083.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTLAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3362..>3561)
                     /locus_tag="VVL53_001975"
     CDS             complement(3362..>3561)
                     /locus_tag="VVL53_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU4184084.1"
                     /translation="LVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYL
                     LTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1189 a          724 c          473 g         1175 t
ORIGIN      
        1 tgtcttccaa acttctttat tccatattta atgaaatgcc accttaaccg tgataatagc
       61 tagtcatcaa taaaaaacta tttgaataag gattctccat ttgattcaat cacttcttta
      121 taccaagtaa aagacatttt cttatatcga tttaatgtac cacttccatc atcgtttcga
      181 tcaacataaa tgagaccgta ccttttagaa agttgtgcag tggacataga aacacagtca
      241 atacatcccc aagacgtata gcccataatt tcaacaccat cctgtagagc ttcagcaact
      301 tgcaataaat gttctttcat atactgaatt ctataatcat cttggacggt taagttatta
      361 agttcatctt ttattagttg atctttagca cctaatccat tttctactat aaataatggg
      421 atttgataac ggtcataata tctatttaaa attatacgta gtccaattgg atcaatttgc
      481 catccccact ctgaagactc taaataagga tttactaaac caccaataat attcccttct
      541 cctgaattat actgtgttgg aagagcagat tgagtcacac tcatgtaata gctaaaggat
      601 aaaaaatcta cggtataatt ttttaataac tctgcatctt cagctgcaaa ctctatgtta
      661 atgtcatttt ccttaaaata tctttttgca taattcggat aataacctct aacatgcaca
      721 tctgaaaata gataatttag attctcatac tcatgagtcg cccatacatc ttttggattt
      781 ggagtcattg gataagctgg catagctaat accatacatc ccaccttaaa ctctgaatta
      841 atctcacgag caatttttgt aaccaaactt gaggcgacta attcatgatg tatagcttga
      901 tataattctt gtttcgaaag attctcctta ggtatatcta ttcctccact agtaaatggt
      961 aattccaaaa cagagtttac ttcgttaaat gtaagccaat atttaacttt atctttatac
     1021 ctttctaaaa ctgttcgagc aaatttttca taaaaatgaa tcattctcct atcaacccat
     1081 ccatgatatt ttcttgctaa atataatgga gtctcatagt gtgaaagagt tacaagtggt
     1141 tctatcccgt gagcatgtag ttcatcaaac aattcatcat aatatttcaa cccagcttcg
     1201 ttaggttctt cctcatctcc ttttggaaaa attctactcc atgcaataga agtacgaaaa
     1261 acattaaagc ccatttcaga aaacaaggat atatcttcct tatatttatg ataaaaatca
     1321 atacctatca attttaagtt atcttctgta ggattttctg ttgcttctcc taatccacct
     1381 ttgggtaaca catcctgaac tgataagccc ttaccatctt cattatatgc tccctctact
     1441 tgattagctg caacagctcc accccaaaga aaatcatctg gaaaaatggt cataactttc
     1501 ctccattata atattaccag taattcctta gaatgctcga ttgtctgatt attaggtaat
     1561 actaatacat ctagaaaatc attggtattc gttacaatta ctggtgtaac tgtttcgtag
     1621 cctttagtct tgattaaatt caagtccatt tcaaaaatca actgattttt gaaaactctg
     1681 tctccttctt ctacatgact aataaaacct tgacctttta gctcaacagt atctaatcca
     1741 atatgaatta gtaactcaac accctcatca ctcttcaatc caattgcgtg cttagtcgga
     1801 aaaatatttg taattttccc atcaaatggt gcataaacct taccttcact tgggataatc
     1861 gctactccgt ctccaattag tttatctgaa aatgttttat cctggacatc gcttaacgga
     1921 atgatttctc ctgatatagg agaaaatatc atttttttat ttgaaactcc agcttcaact
     1981 tctaaattgc tagaactctc ttcttcatcg attccaaata tataagctaa tacaaaggta
     2041 ataacaaccg aaatgaccgc cacaattaaa gcatttacaa tatttgatgg cacatcagaa
     2101 taaataaatt gaggcaacgc tatcaaagat gggacagcaa atagatatgc tttaacacta
     2161 gtaagacctg caaataatcc cgctaatcca ccaccaatca tagctgcata aagcggtttt
     2221 ttatatttta aagtcacacc atataatgca ggttcggtaa tccctgcaag taaggctgag
     2281 aaacctgctg caaaagcaat ttgttttgta ttattatttt tactctttaa tgcaacagcc
     2341 atcgaagcag ccccttgagc taagtttgac cctaacattg ctggaagaat taatacgtct
     2401 ggagtagcaa tagatgccgc caaaaaaata ggtgcaaaag cccaatgcat tccagtcata
     2461 acaataaatg gcataatagc accaagaata gctaatgtaa gccatccagc tacaccatac
     2521 atttgcccaa ctagatttga taatccttca ccaacaatta ctccaatagg tccgactaca
     2581 actaaggcaa tacagcttga tactaataat actagcgtag gttgcaaaaa actcttagta
     2641 atagctagtg ttaatttagc aattattttt tcaatatatt tcatcaacca aaccataata
     2701 agaattggaa cgactgatga accataacta gctggtgtca caggtgcacc aaataaacta
     2761 agaggattcc ctgattgcac catttgaaca aaatttggat ggagaagtac acctgctaca
     2821 gacatagcta atgtagatgt tacttttaat ttttgtgatg cagaataagc taataacagc
     2881 ggtaagaaat aatatggagc atccccaaaa aatgtcaaaa aagcaatagt ctgagaatct
     2941 gattgcaata taccaagcat tggtaaaatg attaccaaga ctttcaacat acctccccct
     3001 aacattgctg gaatgattgg agtcatggaa ccagcgatat actcaatgat tctttctaaa
     3061 atattccctt tgtgcccttg aacaactgaa tcggattcaa aattgccaag tttaacgaat
     3121 tctttataat aattagctac atcattacca agtataattt gatattgtcc attctttttc
     3181 ataataccta ttacacctgg tatcttcttc acatcatcat cattgactaa attttcatct
     3241 tttaattcta atcttaaacg tgttacacaa tgggtaactc tattgacatt tttttcacct
     3301 ccaattacat cgaggatttt ttgtaccgta tctttataac tcatggtatt ctcctattct
     3361 attaatctaa attttttgtt aagcgacgaa tatgagccat caaataaact aattcactag
     3421 aagtcagcaa ataattgtac tccgtttgta taaacattgc tacctgttca ccacattcat
     3481 attctctagg atatttattt ttcattaatg ctaacaagtc ttcatcatca tcgtcacaat
     3541 aagttgtatt ttcaaccaag c
//
LOCUS       DAVHEW010000126         3498 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVHEW010000126 DAVHEW010000000
VERSION     DAVHEW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.126"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            54..281
                     /locus_tag="VVL53_001976"
     CDS             54..281
                     /locus_tag="VVL53_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184085.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            569..3466
                     /gene="nanA"
                     /locus_tag="VVL53_001977"
     CDS             569..3466
                     /gene="nanA"
                     /locus_tag="VVL53_001977"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU4184086.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKKDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1189 a          594 c          800 g          915 t
ORIGIN      
        1 atgcgaaata acagatagag agaagggatt gaagcttaga aaaggggaat aatatgatat
       61 ttaaggcatt caagacaaaa aagcagagaa aaagacaagt tgaactactt ttgacagttt
      121 ttttcgacag ttttctgatt gatttatttc ttcacttatt tgggattgtc ccctttaagc
      181 tggataagat tctgattgtg agcttgatta tatttcccat tatttctaca agtatttatg
      241 cttatgaaaa gctatttgaa aaagtgttcg ataaggattg agcaggaagt atggtgtaaa
      301 tagcataggc tgatgtccat catttgctta taaagagata ttttagttta attgcagcgg
      361 tgtcctggta gataaactag attggcagga gtctgattgg agaaaggaga ggggaaaatt
      421 ggcaccaatt tgagatagtt tgtttagttc atttttgtca tttaaatgaa ctgtagtaaa
      481 agaaagttaa taaaagacaa actaagtgca ttttctggag taaatgtctt atttcagaaa
      541 tcgggatata gatatagaga ggatcagtat gaatcggagt gttcaagaac gtaagtgtcg
      601 ttatagcatt aggaaactat cggtaggagc ggtttctatg attgtaggag cagtggtatt
      661 tggaacgtct cctgttttag ctcaagaagg ggcaagtgag caacctctgg caaatgaaac
      721 tcaactttcg ggggagagct caaccctaac tgatacagaa aagagccagc cttcttcaga
      781 gactgaactt tctggcaata agcaagaaca agaaaggaaa gataagcaag aagaaaaaat
      841 tccaagagat tactatgcac gagatttgga aaatgtcgaa acagtgatag aaaaagaaga
      901 tgttgaaacc aatgcttcaa atggtcagag agttgattta tcaagtgaac tagataaact
      961 aaagaaactt gaaaacgcaa cagttcacat ggagtttaag ccagatgcca aggctccagc
     1021 attctataat ctcttttctg tgtcaagtgc tactaaaaaa gatgagtact tcactatggc
     1081 agtttacaat aatactgcta ctctagaggg gcgtggttcg gatgggaaac agttttacaa
     1141 taattacaac gatgcaccct taaaagttaa accaggtcag tggaattctg tgactttcac
     1201 agttgaaaaa ccgacagcag aattgccaaa tggtcgagtt cgcctgtatg ttaatggtgt
     1261 tttatctcga acaagtctga aatctggcaa tttcattaaa gatatgccag atgtaacgca
     1321 tgtgcaaatc ggagcaacca agcgtgccaa caatacggtt tgggggtcaa atctacagat
     1381 tcggaatctc actgtgtata atcgtgcttt aacaccagaa gaggtacaaa aacgtagtca
     1441 actttttaaa cgctcagatt tagaaaaaaa actacctgaa ggagcggctt taacagagaa
     1501 aacggacata ttcgaaagcg ggcgtaacgg taaaccaaat aaagatggaa tcaagagtta
     1561 tcgtattcca gcacttctca agacagataa aggaactttg atcgcaggtg cagatgaacg
     1621 ccgtctccat tcgagtgact ggggtgatat cggtatggtc atcagacgta gtgaagataa
     1681 tggtaaaact tggggtgacc gagtaaccat taccaactta cgtgacaatc caaaagcttc
     1741 tgacccatcg atcggttcac cagtgaatat cgatatggtg ttggttcaag atcctgaaac
     1801 caaacgaatc ttttctatct atgacatgtt cccagaaggg aagggaatct ttggaatgtc
     1861 ttcacaaaaa gaagaagcct acaaaaaaat cgatggaaaa acctatcaaa tcctctatcg
     1921 tgaaggagaa aagggagctt ataccattcg agaaaatggt actgtctata caccagatgg
     1981 taaggcgaca gactatcgcg ttgttgtaga tcctgttaaa ccagcctata gcgacaaggg
     2041 tgatctatac aagggtaacc aattactagg caatatctac ttcacaacaa acaaaacttc
     2101 tccatttaga attgccaagg atagctatct atggatgtcc tacagtgatg acgacgggaa
     2161 gacatggtca gcgcctcaag atattactcc gatggtcaaa gccgattgga tgaaattctt
     2221 gggtgtaggt cctggaacag gaattgtact tcggaatggg cctcacaagg gacggatttt
     2281 gataccagtt tatacgacta ataatgtatc tcacttaaat ggctcgcaat cttctcgtgt
     2341 catctattca gatgatcatg gaaaaacttg gcatgctgga gaagcggtca acgataaccg
     2401 tcaggtagac ggtcaaaaga tccactcttc tacgatgaac aatagacgtg cgcaaaatac
     2461 agaatcaacg gtggtacaac taaacaatgg agatgttaaa ctctttatgc gtggtttgac
     2521 tggagatctt caggttgcta caagtaaaga cggaggagtg acttgggaga aggatatcaa
     2581 acgttatcca caggttaaag atgtctatgt tcaaatgtct gctatccata cgatgcataa
     2641 tggaaaagaa tacattatcc tcagcaatgc aggtggaccg aatcgtgaaa atgggatggt
     2701 ccacttggca cgtgtcgaag aaaatggtga gttgacttgg ctcaaacaca atccaattca
     2761 aaaaggagag tttgcctata attcgctcca agaattagga aatggggagt atggcatctt
     2821 gtatgaacat actgaaaaag gacaaaatgc ctatacccta tcatttagaa aatttaattg
     2881 ggaatttttg agcaaaaatc tgatttctcc taccgaagcg aaagtgaagc gaactagaga
     2941 gatgggcaaa ggagagatgg gcaaaggagt tattggcttg gagttcgact cagaagtatt
     3001 ggtcaacaag gctccaaccc ttcaattggc aaatggtaaa acagcgactt tcctaaccca
     3061 gtatgatagc aagaccttgt tgtttgcagt agataaggaa gatatcggac aggaaattat
     3121 tggtatagct aaaggaagca tcgaaagtat gcataatctt cctgtaaatc tagcaggtgc
     3181 cagagttcct ggcggagtaa atggtagcaa agcagcggtg catgaagttc cagaatttac
     3241 agggggagtt aatggtacag agccagctgt tcatgaaatc gcagagtata agggatctga
     3301 ttcgcttgta actcttacta caaaaaaaga ttatacttac aaagctcctc ttgctcagca
     3361 ggcacttcct gaaacaggaa acaaggagag tgacctccta gcttcactag gactaacagc
     3421 tttcttcctt ggtctgttta cgctagggaa aaagagagaa caataagaga agaattctaa
     3481 acatttgatt ttgtaaaa
//
LOCUS       DAVHEW010000127         3423 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVHEW010000127 DAVHEW010000000
VERSION     DAVHEW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.127"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(1..1649)
                     /locus_tag="VVL53_001978"
                     /pseudo
     CDS             complement(1..1649)
                     /locus_tag="VVL53_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(1752..2617)
                     /locus_tag="VVL53_001979"
                     /pseudo
     CDS             complement(1752..2617)
                     /locus_tag="VVL53_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            3165..3423
                     /locus_tag="VVL53_001980"
                     /pseudo
     CDS             3165..3423
                     /locus_tag="VVL53_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1126 a          760 c          524 g         1013 t
ORIGIN      
        1 cagaaactat actagctgat tccaaacgta atatggtcat ctcatctacc ttccctaata
       61 atggcattgc accataccaa acaatagtct tattcaccaa aagaataggc gttactattt
      121 ttgattgagt aaaagacact ttaattttct ggctcttcaa ctggtctagc catgctttat
      181 ttgcaaccgt ctctggtata catatctcaa tttgactaat cctaaattct tttagaaaat
      241 tcatcagttt tgtctggtgc acataaggta aaataagcag acattcctgt ctttcccctg
      301 ctaagtcctc tatcaacata aacgaattgt ttctcctcac cctcgatgac acgataatcc
      361 atctttcgat aagctacttg tcgtttctga aacatctttt ctaaataagg aacatgaata
      421 tccacataat cgaaaatacg caccaaagac ttatccttgt agtttctatg aatccgacct
      481 gcatactgaa tcaaattatt tttccaagaa aagggtgctg ccaagataag cgtgtccaac
      541 tgaggtaagt caaaaccttc gccaatgtat tttccagtag acaacaaaac aaacccttta
      601 tctaactgtt ctaacgtctc cagtaaactc gttctctctc ggactttggt ttttccgcta
      661 ataatgtaac agtcatcaac ctctttctct ttcaataatt tttcaaagac atctatctgt
      721 tgaattcgat taactaaaac caagatattc cgtccttctg ccacttgggc tagaatatcc
      781 ttgagaatca tctgattcct cactgagtca gtagcaatcc aatcactaag ctgtataaaa
      841 ttacttgctt tggtcttttc aatttccaaa tgaccaaaag aagtgaatct taattgcaat
      901 tgccgtttaa aatccgtttc cctcttatca gcagtatgga gtatctcacc aattctctga
      961 aaaacaatag gctcatgacc attcttacgc tcaggcgtag ccgtcaaacc gtaaagatac
     1021 ttccctctaa actgagcaac aactttttca aacatcaagg cagagacatg atgacactca
     1081 tccacaatca tcatctcata ctcatccaaa agactttgac tattttccaa cttaaataga
     1141 gattgaatca taacgacatc aaccagttta ctcagccatt tcttagtccc accgtactgc
     1201 ccaacatagc cgattacctt ttcacgacct gatgccgtat aacggatagc ctcctcctct
     1261 tcgaaagtca aaaagcagtt taagcgatct agccattggt ctaagagttg cttattatgg
     1321 actagaataa ttgtttttgt tttccgttca gagataagag cagcacctaa aacggtcttc
     1381 ccaaaaccag tctccgcatg aagtaaacca ttttctttag aagtcatatc tgacagggct
     1441 aactcttgct caaaagtgag ttctccctta aattccacgc taatagacct ttgtaccttt
     1501 ctcctatctt ccacagatac ctgcttaaat ttatcttgca atggatacag caaacctctt
     1561 ggcaaccata aataatgatc ggattctcca aataaataca ttctctcagg aatttgatag
     1621 gttggctgtc gcatagcctg ctttaaatat agtaaaatga aataataaca ggacaaatcg
     1681 atcaggaaag tcaaatcgat ttctaacaat gttttagaag cagaggtgta ctattctagt
     1741 ttcaatctac tataaaattc gggattagaa aaggaagcca tatttttcaa gaaaaaagtg
     1801 ccttggatgt cacagacgat ttttcaagtt ggatcatatt ggataaaacg accttcaact
     1861 ccttatccaa ttcttgcttg cctaactcct cttggattaa cagtgccact ttagcagttg
     1921 aaatcctctg aatttcttgt agatacctcc attggtcttc ataaggctga aactgttcat
     1981 ccacaaagac cgttcgccct tgatggtaag cttctccttg aaaaggcaag gcaatcaaat
     2041 ttccaaatcc ccccttagga aggacatcct gatttggaaa catgcgatca aaagaatcaa
     2101 aggacagttg catactttcc tgcattgcca gttctatcag tttctttcca aacaagcgag
     2161 cctctcgact cggaatcgct tcctcaaaga agaaccaaat atggagtccg tgacccgaac
     2221 gagaaatctc taaatgggct tccatctggc gttccctggc tattcttcga atggttaaac
     2281 cagcttcttt ccaatctcct tcatccaaat ccagtaccaa aaaataacag ctatcatcta
     2341 agtgcatagg aaagataccg atagctgcct ccccacgaaa atgagatttc aaaacggagt
     2401 tcgtcaatgt ctggaaactc cgtttttcag gtggcaactg cttccaacca taatcatagg
     2461 aaggaaaata ctgaattttc ccttgttcat tgataaaatt cttagcataa acatcataac
     2521 ggccagcaaa cacagaggta aacaattgta atttttcttg cgtcgtcaag cgactacttt
     2581 gaagttggaa catctcctcg aactgagagc gttccataac aaactgactc ttgtctttaa
     2641 gacgtttata gtggaatgaa ataagatatg aacaaattga ttaggaaaat caaattaatt
     2701 tatagaaatc ttttagcagt catggcgtcc tattccagat tcaaaatgct atacacaaga
     2761 aaaactcact atccccatta aaagaagaaa agacatcaag gacttctacc acaattccat
     2821 ccaaagacac aaaaacagcc ataagagtca cctccttgat tcctataggc tgattataac
     2881 aagactggct gaaattgtac atgaaaataa aatcctaata gtactcattt tgtatgtgac
     2941 taatattccg tctcgctcca gaaggtacga agtaaataga gtttaaacga gcagtctata
     3001 aaattcaaaa aacgcatatt atcaagcgac ctaaaacttt gggctctata atatttgtag
     3061 tgggtaaatt ccctatggat attatggagc ctattttggg tagaaaaaaa gtcccataag
     3121 atctataatg aaaagcgaca aaacaactca ttagaaagaa tcatatggaa caattacatt
     3181 ttatcacaaa attactagac attaaagacc ctataaaatt ccttaccttg aaacgactgg
     3241 tatgcctact agaattctcc ttagaaaacg tcgttttaag tgctatcatt gcttgaaaat
     3301 gatggtcgct gaaacttcta tcgtcaagaa aaatcaccaa attcctcgta ttatcaacca
     3361 aaagattgcg caaaagttaa ttgaaaagat ttctatgacc gatattgccc atcagctggc
     3421 cat
//
LOCUS       DAVHEW010000128         3377 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVHEW010000128 DAVHEW010000000
VERSION     DAVHEW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.128"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            63..605
                     /locus_tag="VVL53_001981"
     CDS             63..605
                     /locus_tag="VVL53_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4184087.1"
                     /translation="MKGELTYEDKVIQKIIGLSLENVSGLLGIDGGFFSNLKEKIVNS
                     DDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSEIREIVSSEVAKMTDLEIVEINV
                     NVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQFEKAKSGLGSGFSTVQEKVSEG
                     VEAVKGAANGVVSHENTRVN"
     gene            636..839
                     /locus_tag="VVL53_001982"
     CDS             636..839
                     /locus_tag="VVL53_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU4184088.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(950..1222)
                     /locus_tag="VVL53_001983"
                     /pseudo
     CDS             complement(950..1222)
                     /locus_tag="VVL53_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1367..1813)
                     /locus_tag="VVL53_001984"
     CDS             complement(1367..1813)
                     /locus_tag="VVL53_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4184089.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVQKYSRLKLE"
     gene            1883..2542
                     /locus_tag="VVL53_001985"
     CDS             1883..2542
                     /locus_tag="VVL53_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU4184090.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACGIIFGKLLL
                     V"
     gene            complement(2594..>3377)
                     /locus_tag="VVL53_001986"
     CDS             complement(2594..>3377)
                     /locus_tag="VVL53_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU4184091.1"
                     /translation="SESYHKGESIEELALYAREKLGISKDNHDLLYKLERSGIYIVER
                     LINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMNEDGNRLE
                     QEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEYRAFKLGL
                     LTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSLATIMSKQ
                     SIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
BASE COUNT         1011 a          660 c          590 g         1116 t
ORIGIN      
        1 caaacgaaaa aaacacaaac actaacgtag aaaagaaaga tgctactgtt gtagctcacg
       61 aaatcaaagg ggaacttact tacgaagata aagttatcca aaaaatcatt ggtctttcac
      121 tagaaaacgt ttcaggtctt ttgggaatcg atggtggttt cttctcaaat cttaaagaaa
      181 aaatcgttaa cagcgatgac gtaacaagtg gtgttaacgt agaagttggt aaaacacaag
      241 ttgcagttga cttaaacgtt attgttgagt accaaaaaaa tgttccagct ttatattcag
      301 aaatcagaga aatcgtatct tcagaagttg ctaaaatgac tgacttggaa attgttgaaa
      361 tcaacgtaaa cgttgtcgac atcaaaacta aagaacagca tgaagcagac tcagtaagcc
      421 ttcaagatcg cgtatctgac gttgctgaat caacaggaga attcacttca gaacaattcg
      481 aaaaagctaa atctggtctt ggatctggtt tctcaactgt tcaagaaaaa gttagcgaag
      541 gtgtagaagc tgttaaaggt gcagcaaatg gtgtagtatc tcacgaaaac actcgtgtaa
      601 actaagataa aataaatata acaggagaaa ttatcatgtc agtagaagaa aaattaaatc
      661 aagctaaagg ttctattaaa gaaggtgttg ggaaagccat cggtgatgaa aaaatggaaa
      721 aagaaggagc agctgaaaaa gttgtttcta aagtaaaaga agttgccgaa gacgctaaag
      781 acgctgtaga aggtgctgta gaaggtgtta aaaacatgtt gagtggcgac gataaataag
      841 gttaaaagtt actttatctt ttttggtaac attagtcaaa agagtctgag tcaagatgat
      901 tctcagaaaa caaaaagcta gagattccca attgcggaac tctagctttt taattttgcc
      961 tctttctctt attatatttc agcaggttgt tggccatgag tatgaatccc atgtcaattc
     1021 tcacttgacg cttacctctc agatgacatc tcttataacc caaacaaacc tttatctgcc
     1081 caaagacaga tttcatatca atcttacgtt tagcgaaaat ttgtctaccc ttggaagata
     1141 aaagtgcctg atattcttta gtttttaaac actggtaacg ttcattcata tacagtccct
     1201 tttgaggggc tgattcaggt tcataatcgc agtcaacatt gatttcaagg ctgtttgctt
     1261 tctatctccc ctttttcata atgtatagta aaatgaaata ataacaggac gaatcgatcg
     1321 ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca
     1381 atctactata tttttgtaca ggtgcttcaa ccatttgaac gatttcaaat ccttcttttt
     1441 ggtaaagatt ctgagctctt tgatttgcct cgaagacatt tagagaaata ctatctatat
     1501 cttcattttc aaaggccaaa ctaacaaatt tccttaaagc ctggctacct aagtcttgct
     1561 cctgtttctg ggggttgata aaaaatctcc cgatatgaag attgctgtct tctagcctga
     1621 ttttctggat aagtcccaca aactcttgtt catcaaagat tgaaaagact ccttccaagg
     1681 cttgaagggt cagtagaaaa ggaatccttg gtcccatcca ttgttcttga aaggatttgc
     1741 ctagggagtt ggaccactgg catacaaatt gagcgttttc tatactcacc ttttcttcaa
     1801 aacgaattgt catcttttcc tcaccatctt atctatgttt ctccattata ctatttctcc
     1861 cattttttac gaatagataa gtatgattga tttttatttt tttctcgtcg ggagcattct
     1921 cgcttccttt cttggtttgg tcattgaccg ttttccagag caatccatta tcagttcagc
     1981 cagtcactgc gattcctgtc agactcgctt gcgtccctta gatttgattc cgattctctc
     2041 acaggtcttc aatcgctttc gctgtcgcta ctgcaaagtt cgctatcctg tctggtatgc
     2101 cctctttgaa ttagtcttag gactcctctt tctgctttac tcttgggaat tgctttcctt
     2161 ggggcaagtc gtcctaatca ccgctggttt gaccttgggt atctacgact ttcaccatca
     2221 ggaatatccc ttactggtct ggatgacttt ccacctaatc ctaatagctt cctctggctg
     2281 gaatctggtc atggtctcct tccttgctct tggaattttg gctcatttta tcgatatccg
     2341 catgggcgca ggggatttcc tctttttagc ttcttgtgct ctcgtcttta gcgtaacgga
     2401 gttactgatc ttgattcagt ttgcttctgc gacgggtatc ctggcctttc tcctgcaaaa
     2461 gaaaaaggag agacttcctt tcgtgccttt cctcttactt gctgcttgtg ggattatttt
     2521 tggtaagcta ctgcttgtct gataaaatcc aatttctgcc atatgtcctt catgaaatta
     2581 tttcacagtt aaattataaa ttatttcttt tgtacaaagg gatgatgtta tcaaatcgat
     2641 ctgttcttct atcttcttga tactgatcaa aaaatttcat ttcgactgaa aatatttcgc
     2701 ttataaactg taaacgaata ctttgtttag acattatagt cgctagactg actagatgat
     2761 tactcaaaac gacgtccaga atactcttta ctttgcttgg ttttttaaca aaaatttgat
     2821 catccaaagg ttcaatcatt ttgtaacctt tttgcgcaat ttgacgataa aagtaagaat
     2881 gttgctttgg agtcaataat cctaacttaa aagctcgata ctctaaagcc tgtatcgaaa
     2941 cattcaaatc cgacttcaat aaaatataac tatcaggatt gctgacacgc ttgccaaccc
     3001 tctcttcaaa tttgactaaa aactcttctt ttggcaataa aaaacatgat gcaaaataat
     3061 ttgcttcttg ctccaaacga ttgccatctt cattcatatc tttatattta tgtaaaagaa
     3121 tatgtcctag ctcatgagct aagtcaaaat ttcgacgtac agatgattta ttcgttccca
     3181 acacaatata aggtcttccc aattttgacc atgcgctata agcatcagct tggccattaa
     3241 ttaatcgttc cacgatatag atgcctgaac gttctaattt ataaagcaaa tcatgattat
     3301 cttttgaaat acctaatttt tccctggcat aaagagccaa ttcctcaatg gattctccct
     3361 tatgataaga ttcactc
//
LOCUS       DAVHEW010000129         3286 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVHEW010000129 DAVHEW010000000
VERSION     DAVHEW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.129"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            11..310
                     /locus_tag="VVL53_001987"
     CDS             11..310
                     /locus_tag="VVL53_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184092.1"
                     /translation="MQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKSYDEKSHEE
                     LVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVVEKYGH"
     gene            300..2291
                     /locus_tag="VVL53_001988"
     CDS             300..2291
                     /locus_tag="VVL53_001988"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU4184093.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            2307..2783
                     /locus_tag="VVL53_001989"
     CDS             2307..2783
                     /locus_tag="VVL53_001989"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU4184094.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            2817..>3286
                     /locus_tag="VVL53_001990"
     CDS             2817..>3286
                     /locus_tag="VVL53_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184095.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESG"
BASE COUNT         1138 a          430 c          630 g         1088 t
ORIGIN      
        1 attttcgaca atgcaagata ttgagaatgt tgcaaccgat attataaaat catatgataa
       61 tgagatttat acttataaag ctgtttccca agaagaattg gaaaaactag aaaaaagtta
      121 tgatgaaaaa agtcacgaag aattagtttc aatagaaagc aatttagaaa tgaaacaaca
      181 gaaccttatt gatgaggtta ataaaacaat caaggaaaat gatgcaaata ttcagtatat
      241 ttcatcaagt aggagaggag aatttgtaga aaaaattatt ggtagggtgg tagaaaaata
      301 tggccattag tcagatgaaa agaatctctc tactattttc taaaagtagt cttgatgatg
      361 ttttaaaaac tattcaagaa ctagagtcag tgcagttccg tgatttaaag gttcaggata
      421 actggtcaga agctctagaa aaagatgaag ttgtatttcc aactattcaa atttttcata
      481 cttctaattc caatcatggg gttattgagg gaaatgatgc cttgacttat ttgatgaatc
      541 aacaacaaca tttagaagca actgtagaga aattacaaga atacctaccg aaagaaaaca
      601 cgtttaaatt attgcagcaa cctccgataa ctacctctta tgaagaatta gagaaatttg
      661 gtaaagctaa tgttgctgag ggtgttctta aaaaagtgaa tcatcaaatt aacagagttc
      721 atgaattaga aagacacatt caaagtaata atgaggaaat agagcgatta ataaagtggg
      781 aaaaattaga aattgttcct gcgaatttag aacaattttc tttctgtaaa ggaaaagtcg
      841 gaacaattcc aaggactgaa gataatcgct tatacaatag tcttttagaa aacaatattg
      901 aagttcaaga aatattttct aatgatagag agtacggtgt tgttgttttc tatcagtcta
      961 gttactctat agattttgat gaatacttat ttgaaccatt tgattattct agaaaggaat
     1021 taccgaagca gcgagtagta gatttagatc aagaaaacat gcagttaata actgaaaaag
     1081 agaatattat cgcatcgttg caagattcaa agaaatattt gatagattta caatggcaaa
     1141 tagactatat tttatctatc tatgctcgtc aaatctctaa gaataacttt ttgtgcactc
     1201 cgcatctagt tgcattagaa ggatggatag aagaaactcg tattttatat tttataaaag
     1261 ttatggatga gcattttgga cattctattt atatttatga atcggaaaca ttgacggata
     1321 atcaagatga aatacctatc aaattaacga atcattcttt aattgaacca tttgaattat
     1381 tgacagaaat gtatgctctg cccaaatatt atgagaaaga tcctacacct gtattagcac
     1441 cattttactt tacatttttt ggaatgatgg ttgctgattt aggctatggt ttactattgt
     1501 ttttaggaac aatgttagca ttaaaaattt ttcatctacc ttcagcaact aagagatttt
     1561 taaaattctt taatatatta ggggtagccg ttgcaatttg gggtggaatc tatggctcat
     1621 tttttggata tgagttgcca tttcatctga tatctacaac ctctgatgtc atgactatat
     1681 tagtagtgtc agttgtgttt gggtttatta cagtatttgc aggtttgtta gcttcaggac
     1741 tacaaaaagt aagaatgaag aaatatgcag aagcatataa ttcaggattt gcgtggtgtg
     1801 ttattctgct tggcttgtta tttattgctg ttggaatgtt gatgcctgat atgagaccgt
     1861 tatttgtatt agggaaatgg gtatctattt ttaatgctgt ggggattttg attgtttcta
     1921 ttattcaaac caaaagcttg tcaggtattg gagcaggatt gtttaatcta tataacattt
     1981 catcttatat aggtgattta gttagtttca ctcgattgat ggcattagga ttatctggag
     2041 caagtatagc atcagctttc aatttaattg ttggtttgtt tccgggaata ttggctaaac
     2101 tgacaattgg attagtatta ttcattcttt tacatgcgat caatattttt ctatcgttac
     2161 tatcaggata tgttcatgga gcacgtctga tatttgttga attttttggt aagttttatg
     2221 agggtggagg aaaaccattt caacctttga aggcttctga gaaatatatt aaggttatta
     2281 caaagaatta atggaggata tatataatgg aacatttagc aacttatttt tcaacctatg
     2341 gaggagcttt cttcgctgca ttgggaattg tattggcggt tggattaagc ggtatggggt
     2401 ctgcttatgg agttggtaag gctgggcaat ctgccgcagc tttactgaaa gaacagcctg
     2461 aaaagtttgc ctcagctttg atattgcaat tattgcccgg aacacaagga ttatatggtt
     2521 ttgttattgg aattttaatt tggttgcaat taactccaga acttccttta gaaaaaggcg
     2581 ttgcttattt ctttgtagct cttccaattg ctattgtagg atacttttca gctaagcatc
     2641 aaggaaatgt agcagtagcg ggaatgcaaa tcttggctaa aagaccaaaa gaattcatga
     2701 agggagcaat tttagctgcc atggtagaaa cctatgcaat tcttgctttt gtcgtatcat
     2761 tcattttgac ccttcgtgta taagaaataa atttgcaatt caaaggaggt gtctaaatga
     2821 gcaatttaga aaacttacga gagtctgtta ttgaacaagc tcatgaaaaa gggcgtatga
     2881 aattattgga ttccaaaaag aagattgatg atgaatttga aatgcaaaag tcgctcatta
     2941 taaagaaaaa agaagctgaa catgaacgaa agttaaaaga attgcaacag aaatatcaaa
     3001 taatttttca acaattaaaa aataaggaac gccaatcaac gttagtatca aaacagaaaa
     3061 tattaaaaga actttttcaa tctgctttac tagaaatgga atcttggagt gcagataaag
     3121 aaatggagtt catctatcga attctggaac gatattcaca acaagaggtc atagtaacct
     3181 ttggggaacg gactttagct aaattcaatt tggaacaatt agagaaattg aaattctctt
     3241 ttccaaatta tttatttagt gaacaaccta tctcaaatga atcagg
//
LOCUS       DAVHEW010000130         3227 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVHEW010000130 DAVHEW010000000
VERSION     DAVHEW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.130"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(136..747)
                     /gene="rpsD"
                     /locus_tag="VVL53_001991"
     CDS             complement(136..747)
                     /gene="rpsD"
                     /locus_tag="VVL53_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU4184096.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(942..1994)
                     /locus_tag="VVL53_001992"
     CDS             complement(942..1994)
                     /locus_tag="VVL53_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU4184097.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(1991..2689)
                     /locus_tag="VVL53_001993"
     CDS             complement(1991..2689)
                     /locus_tag="VVL53_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4184098.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2856..>3227)
                     /locus_tag="VVL53_001994"
     CDS             complement(2856..>3227)
                     /locus_tag="VVL53_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4184099.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT          899 a          729 c          624 g          975 t
ORIGIN      
        1 actttggact atatcggtct agttatgtct aaacgattat agctggatac taagagaaat
       61 caaaaaagca ctaagggaga acgtcccaaa gtgcttaata tcaaggcctc aaagcctatc
      121 ttattcaata aaatattaca acattttgtt gtagaattca acgacaagtg cttcgttgat
      181 ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat gaaccttcca acttttcagc
      241 gtcgaatgat acgaatgctg gacgtccaag agtagcttct actgcttcaa ggattgctgg
      301 aactttcaat gatttttcac gaactgagat cacttgacct ggagttacgc ggtatgatgg
      361 gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg tttacgaatt gacgagcttg
      421 acgacgagta gtcgcgagac caagacggta aacaacgtta tccaaacgac gttccaaaag
      481 aagcataaag ttgaaaccta ggattccgcc tttgattttt gtagcttgta cgaacaagtt
      541 acggaattgt ttttcaccta caccgtaagt gaaacgaagt ttttgttttt cagccaattg
      601 caaaccgtat tctgacaatt tagaacggtt gtttggtccg tgttgtcctg gtacgtagtt
      661 acgacgtgcc aattctttac ctgtacctgt aagtgaaagg ccaaggcgac gagcttgttt
      721 ccaagatggt cctgtataac gtgacatatg aatgtcctcc tgatataaat aatatttcgg
      781 tggaaatagt cacttagaaa gccctgattc gtgcagatgc ccttcgccta aacagccaag
      841 gttacttgtc ataagacacc tgttgacgag cttcatgctt tcctgctgat atttcacaca
      901 aaatctattc taccatgaat agctggattt gtaaaggggt tttaggcttt attttcacta
      961 ccagagaggt tgagaacgag ggtaaaggta cttcctagac cgtactggct gctgactgtg
     1021 atttccccac ccaattgatg ggccaattca cgcgcaatcg caagtcctaa tccatgacca
     1081 cctgtcttca tgttacgcga agtttcgaca cgataaaggc gtttgaaaat attttccaaa
     1141 tcctctgggg caataccctg cccttcatcg gtcacactga ttgaaagctg gtccttctcc
     1201 agcttagcca ccacttccag cttggttcct ggagcagaat atttaaaagc gttatcgacc
     1261 agattcacca agatacgaga aagcttagca taatctccct caatccgggc agactctggg
     1321 attacctgca agtggacatc tcttctctcc tgctcaatca aaaactgaaa ttcactcatg
     1381 cactcaatta agagcttgtc cagaaaaata ctgtctttac tggtagtttc cacctgattt
     1441 ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca gtttattgag cctctccgtc
     1501 tggcgtccaa tggttgctag ataatgagct tgctccgact ccttgataat accatccaaa
     1561 atcccttcta ccgtcgcttg gatcgaagtg ataggagtct taatatcatg cgacaactgg
     1621 gcaatcatca agcccttttc tcgttcgctt tcttccaagg aatcaaagct tacctgcaaa
     1681 tcatgggaca tctcattaaa agtttgccct aattgctgaa attctacagg accttgaacc
     1741 tccaaatttg aaggaaaatc cttggccgct acccgcttgg catgctcctt gagtttgccc
     1801 aacgacgtaa agactggcaa taggagaaag agactaatcc cagcaccgac aaggctggca
     1861 acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg caatcagcat tttttgaaca
     1921 gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa tatatccaac caaaatataa
     1981 ctttttagtt tcatgtttgt cctctcggtt tctctatctt atatcccaac ccccaaactg
     2041 tcttaatagt gggcgtttgg tcactactat attttgccag ctcctgtcga agagcatgga
     2101 tatgcacatt caaggtattg gtgtcatcca cgtagtcttc tttccagatc ttttcataga
     2161 ggtctgtctt ggagaaaact cgctctggat tgctagctaa aatccacagc aattcaaatg
     2221 atttaacagt taaatccagc atttcttctc ctatttgaac ttcatgacta ctatgattca
     2281 tttttagatt gccaagggaa atcagctctg tttcgccccc acgatgaagg cggcgcaaaa
     2341 tattgtggac acgcaaaacc agctcacgtg ggctaaaagg cttagcaata aaatcatctg
     2401 ctcccaagct caggccgtaa atcttgtcct gttcactggt cttagcagta ataaatagga
     2461 aaggctgctc tggtgataag tattgaacct cactgattaa atcataacca tccatccgag
     2521 gcatcatgac atctgtgata atcaaatcaa tcggtttttt cttgaacagc tctagcgctt
     2581 ccagtccatc atgggctacc aagacctgat aacctgcctg aattaagtat ctctgatgaa
     2641 tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt ctttcccatc tgtctctcct
     2701 ttgataaaaa cagtgctata ctgctttaag tataacacta tttttattgt tttacgatga
     2761 ttttaatctg ctattgaaag aaagtcctga ttgctgattg agttgttaaa ctagtcttag
     2821 tcagcccact atccatcatt tatttcctta gctgattagt tttcttcttt tttgtttttc
     2881 aaacctagac caccgagtaa acctgcaagc gcaagcccaa ggaaaccaat acttgccatt
     2941 gatgtttgag tctcaccagt atttggtagc atagctttat cctctgacat catcgtatca
     3001 gacatcttgt tagcagaagc agccatgttt tcacctgcca tcgtgttggt agaacttgtc
     3061 atggtgtcag caggcatgct atctgtagta cctgtagcat gattgtgatt catcggagtc
     3121 acgccagaac cagagttaga aggtgataat gaaccatttg ctgtgtctga tgtttcttta
     3181 acatttatct taatagtgac ttttttagtt gctacgatgt tgtccaa
//
LOCUS       DAVHEW010000131         3208 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVHEW010000131 DAVHEW010000000
VERSION     DAVHEW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.131"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            102..413
                     /locus_tag="VVL53_001995"
     CDS             102..413
                     /locus_tag="VVL53_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184100.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            417..1133
                     /locus_tag="VVL53_001996"
     CDS             417..1133
                     /locus_tag="VVL53_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4184101.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1263..2078)
                     /locus_tag="VVL53_001997"
     CDS             complement(1263..2078)
                     /locus_tag="VVL53_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU4184102.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2075..2980)
                     /locus_tag="VVL53_001998"
     CDS             complement(2075..2980)
                     /locus_tag="VVL53_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU4184103.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
BASE COUNT         1088 a          544 c          670 g          906 t
ORIGIN      
        1 ctaaaatctt atcagtgaga agatttctta aagtttattc tttgtagttt catctgtatt
       61 agtacataat ttaacttaga gaaaatagga ggactaatcg aatggaatta aaagatttta
      121 cagaaaaaga acaggaaatg attaagaaaa ggcttacaat gtctaatatt agtgacaaag
      181 aaactactga gaagattctg gcgctagtac cacaagactt gattaagcga atcccgtttt
      241 ttgtcagaaa acatgctaca acacgtacga ttaaacgcat ttcaattgaa caccctgaac
      301 tctacgctgt agctcaaaca agtggtgaga ttccagaaaa aaaacgcgaa gaattgcgtc
      361 agattatcac aactatcttt gaacaaaaga tgaataagca tagtattaag tagagaatga
      421 aaaaatattt tattggcggt ttgggaagca atgcctatca tagcaaggat tttcttcaag
      481 aactagattc gcaggtctat tttctaaatc catatgaaaa gcatcttcga gatgaaacag
      541 aattgaaatc atggtttaaa aatgagattg tagaggaaga atctatctgt ctgataggtc
      601 attctattgg aggcgattta gctcgttatt tcgcatcgga atttgaagaa gtaaagaaac
      661 tgattctttt ggatggtggc tatctagatt tagataagat tttacctttg gatacagagt
      721 tagaggaaac taaaaattat atcaaatctc aaattgtttc ggacttagct cttcttattt
      781 ctaaagagaa gtccgaagca aagcattggt cagaaaatat ggagaaagct gtaagacagt
      841 cctatcactg gaatgttgag tataatagat atgagttggc tataaattat gaaaatatag
      901 aagcgatact ccgcctacgg agaaaaatac aagcttttaa gagagaagtg ggagatacct
      961 tgtttattag tcctcgctat cctaatggag ctacatggag agaggaagcc ctaaaagaat
     1021 tgccagacta ttttaatact atttttttag aaaactttgg ccatgagctt tatactcaag
     1081 cacctaaaga aatcgctagt ctaatgaatg agtggctcgc ttattttcta tgagctactt
     1141 gtattgctgg aaatggtata agatgaaaat ctacaagtca ctaattttca tataacaaaa
     1201 aaacgaacag gctctttttc tgttattcag aagagaactt gttcgttttt atcttgattt
     1261 tcttaaaaag ctctatatac atagggattt tctggtttat cgactttggt aaagctgtcg
     1321 atttccaagg ttggaaggtt ttgtttgagt tctttatggt agtgattgac cagttttcct
     1381 ttggctgtaa tccaggtgtt gttttcatac tgccgggtat tgcccttggt cagcaatcca
     1441 tagacaccag aatctgcgat acagtggata atgccgaatc ggaacagaaa ttgactattg
     1501 gcatgactgg ggtcgttata gacaaagcct gtaaactgga ttgtcttgcc ctcaaattca
     1561 tctggatagt cgtagatagc ctccatgact tccatatagt tttcattagt gatctgaata
     1621 ctatcttggg ataagtattt atccgccgcc gttcgcattt ccttttcata ggctgatttt
     1681 gaaaaataag aactagtatc tggtttcaaa tattggcttg tcgtcccttc acttgtctga
     1741 atagctagat ccgttccttc cgataggggg aaatgataac ctttagcaga aacggtctga
     1801 gaatccaagc taacagttgg gaaagttaag ccgatgacaa gtggaatagc cagaagagaa
     1861 atactcgtca tcttggccaa tcggctgttc agatgactgt gggttttgac ttgcttcatc
     1921 cagatataca attgaacaat tgccaagata aaggaaagca ccatggagat ataggccaga
     1981 taggaatagt gcatgttgat gtactggttt agtttgcccg acagatggag gtaaatagtc
     2041 agttcaaaat agccagctaa aactaaaaat cgaatcatag gatgactcca atcaagagag
     2101 aataggctaa aacgaccaga gtcacaatcg taatgaagtg actaataaat cgtgctttca
     2161 agtaattttt catcatgaga atatttttga tatccagcat tgggccaatg acgagaaagg
     2221 ccagaactgg agccaaaccg aaactcgaga gtagagaagc acctataaag gcatccgcct
     2281 cactacagag cgaaagaaga aaggccaaaa gcatcaagag caggatggca aaaagaggag
     2341 tcgcactgat agaggtcaga atccgagtcg gaacgtagac ctgtattata gaagcaaaga
     2401 ggcagccaaa taccaaataa cgccccgtat caaaaaattc atcaatggcc tgcacaaaga
     2461 cttgaaaaac tttttttgca gaactcaagt aagaaaaatc atgctcatga caagccagac
     2521 gattttcttt ctgaatcggt tcttgccaga aaaatcctag aaaaattcct agtattacag
     2581 ccacaagaat ggaacccaga gctcgtaata gggcgacatg gaaggagttg ccaaaggcag
     2641 aataggtcgc aaaaagaaca atgggattga taacaggtgc tgtcacaaga aaaggaacgg
     2701 ccgtgtaact tggaaccttt ttttccagaa aacgattgat gatggggacg attccacatt
     2761 cacaagaagg gaaaagtata ccgacaaagg tcccaaaaaa gattctcccc caacgatttc
     2821 gagggagaaa atgataaacc ttgtcaggtg tgatataaac ttcaatcagc cctgagacaa
     2881 tgcttcctat cagaacgaag ggaagggctt caatgataat ggagagaaaa atggctccag
     2941 tttgaagtac actagatggg agttgttgaa aagcagtcat ctagtttttc ttttctgctt
     3001 tgactttttt ctctttgtca tcagggaaag taacttgttt caaatcaggg agtttagcaa
     3061 actcttcaaa catcttgtcc aagtcgtctc gttttgtaaa tttgacagcc ataggtgtac
     3121 ctcgattcta gattctacct ctattatact attattttgc ggaaaaggga aaagttaaaa
     3181 tttcaaattg agtagctaag agataata
//
LOCUS       DAVHEW010000132         3195 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVHEW010000132 DAVHEW010000000
VERSION     DAVHEW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.132"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            337..1623
                     /locus_tag="VVL53_001999"
     CDS             337..1623
                     /locus_tag="VVL53_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU4184104.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAVTEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            1638..1949
                     /locus_tag="VVL53_002000"
     CDS             1638..1949
                     /locus_tag="VVL53_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184105.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LEHHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2195..2923
                     /locus_tag="VVL53_002001"
                     /pseudo
     CDS             2195..2923
                     /locus_tag="VVL53_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          997 a          548 c          688 g          962 t
ORIGIN      
        1 ttctaacagt tttatatcag aataataaat aaaaaagcga tattctagac tgtatcaatc
       61 cttttccatt ataggagcaa cagagtggga gtagtcatct aaggactaat ttatgtattt
      121 ttacgagtca gtatcttggg atactggttt ttacttttct agactttttg actacttgtt
      181 aaaactggga taattttcga ctgtttaaca gttattatgc aaagtctaaa agattagaat
      241 tgtcaaaaca atccgtctag gcttgatttt atcctttatt tactataaaa tgagaaggaa
      301 aaatgtcaaa cttttatatt gcaaatagga gaaatcatga caaaaacatt aaaacgtcct
      361 gaggttttat cacctgcagg gactttagag aagctaaagg tagctgttca gtatggagca
      421 gatgctgtct ttatcggtgg tcaggcctat ggtcttcgta gccgtgcggg aaactttact
      481 ttcgaacaga tggaagaagg cgtgcagttt gcggccaagt atggtgccaa ggtctatgta
      541 gcggctaata tggttatgca cgaaggaaat gaagctggtg ctggtgagtg gttccgtaaa
      601 ctgcgtgata tcgggattgc agcagttatc gtatctgacc cagccttgat tatgattgca
      661 gtgactgaag caccaggcct tgaaatccac ctttctaccc aagccagtgc cactaactat
      721 gaaacccttg agttctggaa agagctaggc ttgactcgtg tcgttttagc gcgtgaggtt
      781 tcaatggaag aattagctga gatccgcaaa cgtacagatg ttgaaattga agcctttgtc
      841 catggagcta tgtgtatttc atactctgga cgttgtactc tttcaaacca catgagtatg
      901 cgtgatgcca accgtggtgg atgttctcag tcatgccgtt ggaaatacga cctttacgat
      961 atgccatttg ggaaagaacg taagagtttg cagggtgaga ttccagaaga attttcaatg
     1021 tcagccgttg acatgtctat gattgaccac attccagata tgattgaaaa tggtgtggac
     1081 agtctaaaaa tcgaaggacg tatgaagtct attcactacg tatcaacagt aaccaactgc
     1141 tacaaggcgg ctgtggatgc ctatcttgaa agtcctgaaa agtttgaagc tatcaaacaa
     1201 gacttggtgg acgagatgtg gaaggttgcc caacgtgaac tggctacagg attttactat
     1261 ggtacaccat ctgaaaatga gcagttgttt ggtgctcgcc gtaaaattcc tgagtacaag
     1321 tttgtcgctg aagtggtttc ttatgatgat gcggcacaaa cagcaacaat tcgtcaacga
     1381 aatgtcatta acgaagggga ccaagttgag ttttatggtc caggtttccg tcattttgaa
     1441 acctatattg aagatttgca tgatgccaaa ggcaataaaa tcgaccgcgc tccaaatcca
     1501 atggaactat tgactattaa ggtgcctcaa cccgttcaat caggagacat ggttcgtgca
     1561 ttaaaagaag gactcatcaa tctttataag gaagatggaa ccagcgtcac agttcgagct
     1621 taagaaagga aaaggaaatg atagaggcac agggtttctt agtggataag caaacaagat
     1681 gcattcatta ccatagcaag ctggatatta ttgctttaca atgctatgat tgtaaaaagt
     1741 attatgcttg ttatcggtgt catgattcat tagaacatca cccttttgag ccgtatccct
     1801 tatctttgat acaggataag cctattttat gtggtgtttg tctaaaacta ctaacatata
     1861 agcaatataa agaaagctta agttgcccct tttgtttttc tcgctttaat ccaggttgcc
     1921 aaaatcataa ggaacgctat tttaaatagc aaatcatcta gttttgaagt aggagaaaac
     1981 tcaatttcaa gagaaaatga agtaaatctt cccacaataa aacgcataat atcaagattg
     2041 ttcaatacct gatactatgc gtttttaaga ttttaaagac ttttttcctt tatctggtat
     2101 tttgactact tgttaaaact gggttaattt tcgactgttt aatagttatt atgcaaagtc
     2161 taaaaggtta gaattgtcaa aacaatccgt ctagagtatg cgtgatgcca accgtggtgg
     2221 atgttctcag tcatgccgtt ggaagtacga cctttacgat atgccatttg ggaaagaacg
     2281 taagagtttg cagggtgaga ttccagaaga attttcaatg tcagccgttg atatgtctat
     2341 gattgaccat atctcagata tgattgaaaa tggtgtggac agtctaaaaa tcgaaggacg
     2401 tatggagtct attcactatg tatcaacagt aaccaactgc tacaaggcgg ctgtggatgc
     2461 ctatcttgaa agtcctgaaa agtttgaagc tatcaaacaa gacttggtgg acgagatgtg
     2521 gaaggttgcc caacgtgaac tggctacagg attttactat ggtacaccat ctgaaaatga
     2581 gcagttgttt ggtgctcgtc gtaaaatccc tgagtacaag tttgtcgctg aagtggtttc
     2641 ttatgatgat gcggcacaaa cagcaactat tcgtcaacga aacgtcatta acgaagggga
     2701 ccaagttgag ttttatggtc caggtttccg tcattttgaa acctatattg aagatttgca
     2761 tgatgctaaa ggcaataaaa tcgaccgcgc tccaaatcca atggaactat tgactattaa
     2821 agtcccacaa cctgttcaat caggagacat ggttcgagct cttaaagagg ggcttatcaa
     2881 tctttataag gaagatggaa ccagcgtcac agttcgtgct taatgtagtt gtttagtttt
     2941 aaaaaactat gcaaagctcc atatacaaca cttaaacgag attaaagaat ggcgaaatcc
     3001 cttgatgcgc aagagattag ctgtcttttt tattttttaa gtgataaagt cggagtttag
     3061 gcatcaaagc ctatcaaatt aaacaaagaa gcgatgtctt agatattttg aaaaaaatta
     3121 ataagcagaa aactctctat tattttgttg tagagagttt tttgttaata aaatttcaca
     3181 aaatgacatt tatat
//
LOCUS       DAVHEW010000133         3091 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVHEW010000133 DAVHEW010000000
VERSION     DAVHEW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.133"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(81..614)
                     /locus_tag="VVL53_002002"
     CDS             complement(81..614)
                     /locus_tag="VVL53_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU4184106.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(703..1479)
                     /gene="recX"
                     /locus_tag="VVL53_002003"
     CDS             complement(703..1479)
                     /gene="recX"
                     /locus_tag="VVL53_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU4184107.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1517..2872
                     /gene="rlmD"
                     /locus_tag="VVL53_002004"
     CDS             1517..2872
                     /gene="rlmD"
                     /locus_tag="VVL53_002004"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU4184108.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            2934..3005
                     /locus_tag="VVL53_002005"
     tRNA            2934..3005
                     /locus_tag="VVL53_002005"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2967..2969,aa:Glu,seq:ttc)
BASE COUNT          987 a          558 c          575 g          971 t
ORIGIN      
        1 atttattcct ttttgtgttt acacaattta ttttatacta ccaaaaaagg tcaggatttt
       61 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      121 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      181 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      241 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      301 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      361 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      421 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      481 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      541 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      601 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      661 accataaaat tacccaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
      721 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
      781 cttcagttcg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
      841 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
      901 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
      961 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1021 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1081 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattggcatt
     1141 gatgatagca taagcgtact ggctatcatt aatccacttc tcttctttaa gattagcaat
     1201 gacttgagaa acgatgtttt cattaatatc atattttttc agatattctc tgacctcttt
     1261 ttcagtacgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1321 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1381 aaaacgaaca attgtgtctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1441 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1501 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1561 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1621 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1681 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     1741 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     1801 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     1861 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     1921 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     1981 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2041 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2101 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2161 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2221 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2281 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2341 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2401 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2461 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2521 tccagaagct attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca
     2581 ttatgaagct ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc
     2641 agatgctttg attgttgacc caccacgtac aggtctggat gataagttat tagatactat
     2701 tcttacttat gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg
     2761 tgatttggta cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt
     2821 cccacataca gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaagtag
     2881 ttgacaaagt ttgaaaagac tgtataatag taagagttga aaataacaac tcaggtccgt
     2941 tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg
     3001 gactatggta tgttgcggtt ggaacacttg atgaaaaaaa gtttaaaaaa acttaaaaat
     3061 cttcaaaaaa agtgttgaca agtgaaagtg g
//
LOCUS       DAVHEW010000134         3048 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVHEW010000134 DAVHEW010000000
VERSION     DAVHEW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.134"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            30..1421
                     /locus_tag="VVL53_002006"
     CDS             30..1421
                     /locus_tag="VVL53_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4184109.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            1564..2481
                     /gene="adcAII"
                     /locus_tag="VVL53_002007"
     CDS             1564..2481
                     /gene="adcAII"
                     /locus_tag="VVL53_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU4184110.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            2489..>3048
                     /locus_tag="VVL53_002008"
     CDS             2489..>3048
                     /locus_tag="VVL53_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU4184111.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDG"
BASE COUNT          900 a          538 c          670 g          940 t
ORIGIN      
        1 agaattatca gtttattttg gagtcaaaga tgaatatatt tagaacaaag aatgttagtt
       61 tagataaaac agagatgcat aggcatttga agttatggga tttgattttg ctgggtatcg
      121 gagccatggt agggacaggc gtctttacaa tcacaggtac tgcagctgca acacttgctg
      181 gcccagccct agtgatttca atcgttattt ctgccttgtg tgtgggatta tcagccctct
      241 tttttgcaga atttgcctcg cgagtacccg ctacaggagg tgcctatagt tacctctatg
      301 ctatcttagg agaattccct gcctggttgg ctggttggtt aaccatgatg gagttcatga
      361 cagccatatc aggcgtagct tcgggttggg cagcttattt taaaggcttg ctctctcaat
      421 acgggatagc ctttcctcag gctttaaatg gtacctttaa tcctcaagca gggacatttg
      481 ttgatttatt gcctattctt gtcttggtct tggtgacttc gcttgtttta ctaaatgcca
      541 aggcagcctt acgctttaat tcgattctag ttattttgaa attttccgct ttagctctct
      601 ttgttttagt aggaatttgg aatattaagt ttgataattg gagcaatttt gctccttacg
      661 gttttggaca aatctatgga gctagcactg gtattatggc tggtgcgtcc ttgatgttct
      721 tcggtttttt gggatttgaa tccatctcta tggctgtaga tgaagtcaag actcctcaaa
      781 aaaatattcc tagagggatt gtcttatcgc tttctatcgt aaccattctc tatgccttgg
      841 tgacccttgt tttgactggt gtggttcact atagtcattt aaatgtcgat gatgccgttg
      901 cctttgccct tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca ttagtggcta
      961 tcttgacctt gattacagtt tgtatttcga tgacctatgc cctatcgcgt atgatttaca
     1021 gtttagcacg tgacggctta gtgcctgctg cctttaagga actgacgaag actagcaaga
     1081 taccaaagaa tgctactatc ttgacaggtc tagcttcagc agtagcagca ggaatgttcc
     1141 cactagccag tattgcagcc tttttaaata tttgtacctt agcctacttg atcatgttgg
     1201 cttatggtct gattcgctta cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa
     1261 caccattggt acctctgtta ccaattttat cgatcatcat ttgtttatcc ttcatgttac
     1321 agtataatat gaatacctgg attgctttcc tagttgcgtt gttagtagga agtattattt
     1381 acttcactta tggctataag cattctacca tagaagaata aagtgaaagt ctattgagtt
     1441 gttgacactc agtagatttt tatatgtgaa agaaatttca aattttttct aaaaatatct
     1501 tgacagatta aattttcagg agtagaatat ttactagtta attaaatggt taaggagttg
     1561 ttcatgaaga aacaaaattt atttttagtc ctgttaagtg tctttctttt gtgcttgggg
     1621 gcttgtggtc aaaaggaaag tcagacagga aaggggatga aaattgtgac cagtttttat
     1681 cctatctacg ctatggttaa ggaagtatct ggtgacttga atgatgttcg gatgattcag
     1741 tcaagtagtg gtattcactc ctttgaacct tcggcaaatg atatcgcagc catctatgat
     1801 gcagatgtct ttgtttacca ttctcataca ctcgaatctt gggcaggaag tctggatcca
     1861 aatctaaaaa aatccaaagt gaaggtctta gaggcttctg agggaatgac cttggaacgt
     1921 gtccctggac tagaggatgt ggaagcaggg gatggagttg atgaaaaaac gctctatgac
     1981 cctcacacat ggctagatcc tgaaaaagct ggagaagaag cccaaattat cgctgataaa
     2041 ctttcagagg tggatagtga gcataaagag acttatcaaa aaaatgcgca agcctttatc
     2101 aaaaaagctc aggaattgac taagaaattc caaccaaaat ttgaaaaagc gactcagaaa
     2161 acatttgtaa cacaacatac agccttttct tatctagcga agagatttgg gcttaatcaa
     2221 cttggtattg caggtatctc tcctgaacaa gaaccaagtc cacgacaact aacagaaatt
     2281 caggaatttg ttaagaccta taaggttaaa acgattttta cagaaagtaa cgcttcttca
     2341 aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct gaatccttta
     2401 gagtcagacc cacaaaatga caagacctat ttagaaaatc ttgaagaaaa tatgagtatt
     2461 ctagcagaag aattaaagtg aggaaagaat gaaaattaat aaaaaatatc tagcaggttc
     2521 agtggcagtc cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc aagctggtca
     2581 ggttaagaaa gagtctaatc gagtttctta tatagatggt gatcaggctg gtcaaaaggc
     2641 agaaaacttg acaccagatg aagtcagtaa gagggagggg atcaacgccg aacaaatcgt
     2701 catcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc attactataa
     2761 tggcaaggtc ccttatgatg ccatcatcag tgaagagctc ctcatgaaag atccgaatta
     2821 tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggttatgtta tcaaggtaga
     2881 tggaaaatac tatgtttacc ttaaggatgc agctcatgcg gataatattc ggacaaaaga
     2941 agagattaaa cgtcagaagc aggaacacag tcataatcac gggggtggtt ctaacgatca
     3001 agcagtagtt gcagccagag cccaaggacg ctatacaacg gatgatgg
//
LOCUS       DAVHEW010000135         2878 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVHEW010000135 DAVHEW010000000
VERSION     DAVHEW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.135"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..1669
                     /locus_tag="VVL53_002009"
     CDS             <1..1669
                     /locus_tag="VVL53_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU4184112.1"
                     /translation="KIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYE
                     DAGKSDKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLHIMQDYG
                     VNLICVEDGIDSSKDAGKLMISVLSAVAEIERENISIQTMEGRIQKAGEGKWNGGFAP
                     YGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPL
                     FDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDE
                     VWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKY
                     KDFYYYGCNHRQMIRGNKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMK
                     VDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDK
                     LDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEI
                     QVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            1937..2503
                     /locus_tag="VVL53_002010"
     CDS             1937..2503
                     /locus_tag="VVL53_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184113.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1075 a          364 c          624 g          815 t
ORIGIN      
        1 aaaaataaaa gtatacctct atacacgagt atctacgtca atacagatag aggggtattc
       61 gttagaggca caaaaatcac gaatgaaagc ttttgctatt tacaacgatt atgaaattgt
      121 tggagagtat gaagatgccg ggaagtctga caagtctatt gaaggtagaa tacagttcaa
      181 tcgaatgatg gaagatataa aatctggaaa agatggagta tcttttgttc ttgtgtttaa
      241 gttatcaaga tttgcaagaa atgccgctga tgttctttct acacttcata taatgcagga
      301 ttatggagtt aatcttatct gcgttgaaga tggcattgat tcatctaaag atgctgggaa
      361 actaatgatt tctgttttat cagctgtggc tgaaatagaa agagaaaaca tcagtattca
      421 aacaatggaa ggtcgcattc aaaaagcagg ggaaggaaaa tggaatggtg gctttgctcc
      481 gtatggatat aaacttgaag acggcaagct gtttataaat gaggaagagg cagttgcaat
      541 aagaacgatt ttcgaccagt atgtaaatac tacgatagga gccaatggga tatctaaata
      601 cttagagaat catggaatta gaaaaatccc aagacagaat ggtaagaatc ctttgtttga
      661 tgcaggtctt ataagaaaga tattaaagaa tcctgtatat aatgggaaaa tagcatttgg
      721 aagaagaact ttagaaaaag ttcatggtac aagaaatgaa tataagcagg ttgaacaaga
      781 tgaatatcta atatctgaag ggatacatga agctatagtt tccgatgaag tttggcaagc
      841 tgctcaggtt aagctaaaat ctcaagcaaa gaaatatgag catgtgaata aaggaaaaga
      901 tacacgcaca cacttgcttt ctggaattgt aaaatgcccg atatgtggag tgggaatgtt
      961 tgggaacaag tgtatcaaga aaaagaaaga tggcacaaag tataaagatt tttattacta
     1021 tggttgtaac cataggcaga tgataagagg taataagtgt acattcagta agcaaattag
     1081 agaagaattg ttagatgatg ctgttgcaga ggtgattgtc aagatagtaa gtaatccgaa
     1141 atttgcttct atgatgcaag aaaaaatcaa catgaaggtg gatacctctg aaatagaaaa
     1201 agaaatagat aattaccaaa aggaattgag aaagagtcat tctacaaagt ttaagctaat
     1261 tgaggaaata gataatttag atgttgaaga taagcattat aagagaagga aacaggattt
     1321 agatgataga ctatatcgta tgtatgacaa actagatgaa ttagaatcat cactaattga
     1381 tgcgaaagca aagaaacaga ctattgaagc tgaaaagctt acaggagata acatatataa
     1441 ggttctgatc tattttgata agctctataa agtcatgaat gatgtagagc gtaggcagtt
     1501 aatttcagct ttgatttctg aaattcaagt ttatgaagaa aaacaatcta acggacaatg
     1561 gctaaaatcc attactttta agctaccaat tatagaagaa aatctcaaca taggtttgga
     1621 caatgatgaa caagttgaat gtgtaagttt attagagaaa cgtagttaat cctcaaaagg
     1681 ttccccgaac cttttgagtc ccactgacgc atcacgctga ggcggtatca ctgcttgtac
     1741 gagttgaagc atcagcaaag taaaagtaga tgttcagcca atgcgacgag gtgagaccga
     1801 aggtcgataa ggtgtgttct gggtataaga ctcgtaaagt aagtaggaaa tatgatgaga
     1861 tgatacagag agcctgaatt ggtcaggtga cgtgcttatt tacattagcc tgataatgaa
     1921 aatggagaga ttatcgatgg ttatattgga attgtaccaa aatgattatt ctaaagattt
     1981 agtagcattt gattccatag aagatgggaa agcctttgta gctcaaatcc cgggatacac
     2041 tctggaaaca gaagatggtt ttgaagtgga gtattttaat cctaaaaaca taccggatta
     2101 tatggaaatt atttttaacg gaaatattgt tcctttatct aaatttatgt tcgatcctga
     2161 ggaaaatgta aacattattt ggaaagagat ttcaaattta tccctcaaaa atgatagagt
     2221 gatcgaagga tattcaaaaa ttgatgcata tgtcgttaac aaccatgaag ttaaagccta
     2281 tgtcgaaaca agagaaacga actatcgtaa agcaaaagac ttcttagaaa gccgcggata
     2341 tgaaatcgac agaagctttt tcggaagtga agacggtgaa gcgattcttt acagaaaaaa
     2401 agggatcgaa gtttggcatt tcttgtgtca cttagaccca atgtttgtag agattgaaga
     2461 tgtagaagga tatgttaaag aagaagttgg agagattcaa tagtcaacca aagttccttg
     2521 tgagtatata cctttgctag taaaagagtc aaaacgttat aacgaagggg gggaagaaaa
     2581 cccttgagtg taagtctttt tcttgtttag agggaaagta ttagagtagc cgctcataat
     2641 ttatactaga tttgataact gatttgagac agattttatt tctataaata ttgtgaaaat
     2701 tttttaatac caaagattta aagttactgt gttaaaaatc ctctccactt ttaaactaat
     2761 gtattataaa agccagttga cactagtgtt acacctgtga tagaattaac ttgaggtgat
     2821 gaaaatggca accgataaaa atagaataat gattagcttg gatgataaaa atctagag
//
LOCUS       DAVHEW010000136         2860 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVHEW010000136 DAVHEW010000000
VERSION     DAVHEW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.136"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(272..829)
                     /gene="sdbB"
                     /locus_tag="VVL53_002011"
     CDS             complement(272..829)
                     /gene="sdbB"
                     /locus_tag="VVL53_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU4184114.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(840..1547)
                     /gene="ccdA2"
                     /locus_tag="VVL53_002012"
     CDS             complement(840..1547)
                     /gene="ccdA2"
                     /locus_tag="VVL53_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU4184115.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(1778..2182)
                     /locus_tag="VVL53_002013"
     CDS             complement(1778..2182)
                     /locus_tag="VVL53_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184116.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            complement(2224..2424)
                     /locus_tag="VVL53_002014"
     CDS             complement(2224..2424)
                     /locus_tag="VVL53_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184117.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            complement(2591..2860)
                     /locus_tag="VVL53_002015"
                     /pseudo
     CDS             complement(2591..2860)
                     /locus_tag="VVL53_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          838 a          606 c          514 g          902 t
ORIGIN      
        1 tctcaaagta acttccatct ctctcccaaa actggaagtt actctaagac gactctgttt
       61 tagggctatt tgtcgtgcac tttttctgag tcttttcgct tttgaaccct tatatatcaa
      121 gaaaaagaaa accagtggaa gttagtctaa gacggatttc cactgatgtc acgcgttttt
      181 ctcatactaa aagctaggtt gtgggaaact ggaagttagt tttagaagtt accaaaatct
      241 gactacatct tgtaatcaga ttttatcgat tctagttcat ttctttaaat gctgcttctg
      301 catccgcatt actgatagca ccaaattgaa tctttccaat ctttccttgg ctatcaatta
      361 aatattctgt aggaatgctt cgaatttgat aagcttggaa ggtggttgct ttggtatcat
      421 aaagaactgg gatatcctta tatccttgtt cctggaacca ttgtgggaat tgctcaacag
      481 ttttttcacc ttgaattcct ggtgcaatga cagtaagaat ttcgaaatca cgatctggtt
      541 tcgccgctag ttccatcaac tctggcatac ttttcttgca tggaccacac catgaagccc
      601 aaaacttcaa gtaaaccttt ttacccttaa aatcagataa cttaacttct ttgccatcca
      661 tggattgcaa tgtgaagtct ggagcatctt ttccaacagc aatttgttgt acagtcgttt
      721 gttgttttgg ctgttgtgct gcttgagtct ttttagtttc ttcctcacca caggccatca
      781 atacaactaa tgacaagaga cttaagccag caaacattac ttttttcatt tgtcctcctt
      841 tattcaaaaa ttccagctag aacatttact tgtcctaata gtaacaaaat tcccattaaa
      901 acaatgagga aaccaccaat tttctttagt agcatcatat gacgcttgat tttactaaaa
      961 tatggcatga ctagacctga agctagtgcc aataccaaga aaggaagggc catgcccaga
     1021 gtgtaaatga gagtataaat cgctccttgc caagcgccat tgcctccaga agccgcaagt
     1081 gctaaaacag aacttaaaac tggaccaata caaggtgtcc aaccaaagct aaaggtaata
     1141 ccaagtaaaa aagctgacca ataacgatta gaatctgatt ttttaaaggt aaaacttttt
     1201 tgaacttcta atttcttcaa atgaaaaatt tccatctggt gaagacccaa aatgataata
     1261 atagctccca tgccatatcg aaaccaattt gcatagagaa tatgaccaaa gtaaccagca
     1321 ccaaagccta gaataaagaa aatgagagag ataccagcga taaagcaaag tgttcgaatc
     1381 aagcctgacc agagaacctt tctcccaaac aaagaaaagc tttttgcact ttcttgatca
     1441 tccaataaaa tcccagcata gactggcaga agaggaaaaa tacaaggaga aaaaaaggat
     1501 aaaacacctg ctagaaaaac agagattaaa aatactatcg tttccaataa agaaccaact
     1561 ttcttaataa ttctaatcct attttactat attcaatttt atttgtaagc tttctgctac
     1621 gcaaaatcgt atcgggcact attggaccaa tcttttcttt tgctagtcaa ggcggatctt
     1681 atcccccaaa atagccaaaa agcaacgaca aggattactc atcgctgctt ttgtgaacga
     1741 aaatgtcttt taggtctgac atttcataaa tcatgtttta cttgagtttg tcaaggattg
     1801 ctttaagctc ctctactagt ttagtttctg tctctgctga gccattttct tctttcacga
     1861 aatcaagggt ttcttggaga aggttttggg ctttggcaag gactttttta tccgcttttt
     1921 ctgcatctag ctgtcctaga accttgatca attccgtgct taattgctgg atttctgact
     1981 ctttcttacg gcgaatcagc cagaaggcaa tcacgcctag gagggcaagt agactgacca
     2041 caatcactcc tgccggaact gagtttgttt cagtcatctt atctgaatcc ttactatctt
     2101 ccgttccttg ttttgcatcc ttcttgtcct gtgcaggctt gctgtcgcta gcatttgctt
     2161 tcacatcttt gagagagtcc aaggcagccc agccttcaca gactctactg cagtatgcag
     2221 accttactct gtcaaggcac tatcttccgg agctttttga gcatctagga ggacagcctt
     2281 ggttgcatcg attttcggat cagatactgt tgccaaagct ttcaagcgtt ggtctaactc
     2341 ttgactcaag gcacgaagtt cagacttgtc aacttgctct tgagcttgtg tgctcgttga
     2401 gctagccgaa gcgcttgcta ccactctagg atcttgagtc ggagctgagc ttggagctgg
     2461 gacagggctt gcaggttgac taggaacagt tatggtatat tgaaactaga atagtacata
     2521 tggacttcta aaacattgtt agaattcgat tttactgtcc tgatcgattt gtcctattct
     2581 tatttcattt tactataata accgatggtg tggttaatgt tggtaagaga aacttctgaa
     2641 accaagcttc aaaaaagtcg ctcgtcatcg tctcttcgta agtcattgga gcgattaatt
     2701 caccatttgt tagacctgca accaaagaaa tcctctgata tcttcttcca gatactttgc
     2761 ctcttattaa ctgacctttt aatgagcgac catattctcg ataaaaataa gtatcgaatc
     2821 ctgtttcgtc aatctaaaca ggtgctaggt gctttaaact
//
LOCUS       DAVHEW010000137         2700 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVHEW010000137 DAVHEW010000000
VERSION     DAVHEW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.137"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..295)
                     /locus_tag="VVL53_002016"
     CDS             complement(<1..295)
                     /locus_tag="VVL53_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184118.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFP"
     gene            complement(353..1066)
                     /locus_tag="VVL53_002017"
     CDS             complement(353..1066)
                     /locus_tag="VVL53_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU4184119.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1526..1900)
                     /locus_tag="VVL53_002018"
     CDS             complement(1526..1900)
                     /locus_tag="VVL53_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU4184120.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGGIG
                     TSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            complement(2322..>2700)
                     /locus_tag="VVL53_002019"
     CDS             complement(2322..>2700)
                     /locus_tag="VVL53_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU4184121.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          818 a          530 c          431 g          921 t
ORIGIN      
        1 gaggaaaatc tcccatacag ccattacgat tcatattgag gctatatttt cgattagtaa
       61 gataattcgg aaatatattt cctattactc taagattatc tacttgtgaa tcccttctcc
      121 caatataatc cccttctttg actaatgatg gagatttcca agtaaaaatt aaatcgccag
      181 agaaattttt cttatcaaaa aacttaaaaa gagattgata aatactattt gctaattgac
      241 aagtgtcaga tgtaaattca taattaacag cttcattaaa atcaacttca gacatttcaa
      301 tgtactcttc aatagtaata ttagcttttt tgtagtcatt agctcgtctc ctttaaaatg
      361 tgatcttttc aagaatattt ttatctaaac aatccagcaa gtcttggtaa gaatagactt
      421 cgtaagtcgg ctgggcttgt gtgtgatttt cgaggtgatg aggattatac cagatagtgt
      481 caatccccgc attattgcca ccttgaatgt cggcggttag agaatctcca atcatcagcg
      541 tcttttcttt actaaatcca gcaatttgct ggccaatctt ttcataaaaa agagcatccg
      601 gcttttgagt ttgcaactgt tctgagataa agacttgatt gaaataaggt gctagaccag
      661 attgagccaa acgtcctgtc tgaatggcag taatgccatt tgtcgcagca tacaagttat
      721 aatcacgctc aatgaggctg tccaagagat catgagcgcc cgatagtgtt tgtccctgct
      781 gggcgaggta aaattggtaa cgctgggcaa gaaaactacc gtctttttcc tgtccaaaat
      841 gagcaaataa acgagaaaag cgcgtgttaa ccagctcttg tttactgatt ttcttcagct
      901 ccaagtcttt ccagagagcc ttgttcatag gaacgtaata atctttataa gcctgaatat
      961 ccgcaactcc ttcttctttt agaagttggg tcaaagccac atcctcagca gcatcaaaat
     1021 caagaagagt gtggtcgagg tcgaagagta gaaatttgta gaacaatttg aggttttcct
     1081 ttctgaaaat tcattaagaa cattatatca taaagcacct catacaatta actaatttaa
     1141 tcacttaaaa aaattcgaac actttctata caactgacag ctcaaatctt tcagaataga
     1201 acaatactaa ctatcgaaca ccccgtcttc ataaatatat atatgtaatt ctagacctag
     1261 aattcctata aactaaatgc tttcatactc ttccaagtaa ttgattgcct taaattttaa
     1321 tttttgaagg tttctaaagc tagaatagcc ccatcacaat cagttttgat tgattcacaa
     1381 tttagaaaca ctatagtttc actcctgtta aaataaaaag gaactgcata aagcaatccc
     1441 tttctgattt tgaaatcatt tacttaacat tttatagttg agataatcaa tagcttatct
     1501 ataaaaagag ttatagtaaa attccttatt tattgatttc aagctccgct aactgtgttt
     1561 gaataactga cagttctgca ccagcctgaa gaagagcagc tgcattatag gcaccttcta
     1621 caattggaac cctgttgatg atgatacttt tatcactgaa atcagtcacc atttttaagt
     1681 tcattttagc agaacctagg tcaaaaaagg caagtaaagt atctgctgga ttttcggaaa
     1741 caaccctatc tacttgttca aaactcgttc caattcctcc gccctcggtt cctcttacat
     1801 aagtaatcgg aacatcttta gctactttac taatcagttc aacaacacct tctgcaatgt
     1861 gtttggaatg tgaaacgata acaagaccaa tactttccat caaaccaccc cagtttctaa
     1921 aatagcagta aagagtaatc ctgatgagaa tgatccagga tcaatatgtc caagaaacca
     1981 catgctccta agacaagagc taacagactg gccatcaata atagtattgt tctttttttc
     2041 atcattactc cttaactagt gtttaactga ttaattagcc agtaaatagt ttatctttat
     2101 ttacactatc tgttaagata tagtaaaatg aaataagaac aggacagtca aatcgatttc
     2161 taacaatgtt ttagaagtag aggtatacta ttctagtttc aatctactat attttgcaca
     2221 ttttcatgaa aaaaatgaga actagaactc acattctgct ctcatttttc gttttcccgt
     2281 tctcctatcc tgtttttagg agttagaaaa tgctgctacc tttacttact ctcctttaat
     2341 aaagccaata gtttttcagc ttctgccata atagtattgt tgtcctgggt gccaaatagt
     2401 aaattatttt ttaatcctgt gagagtctct ttggcattgg acttgataat tggatcctgg
     2461 atttttccaa gtaaatcttc agcctctctc agtttttctt caaccttttc agtctcgacc
     2521 tgaggttctt ctgattcctc tggtgattct tctggttcct ctgttggttt tggagactct
     2581 ggtttctcgc tttgcggttt ctcttctcga ggggtttctt cctcaggttt ttctgtctga
     2641 ggtttctcct cgcttggttt ttccgtttga ttggtatcag cttgaccatt tttgtttctt
//
LOCUS       DAVHEW010000138         2678 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVHEW010000138 DAVHEW010000000
VERSION     DAVHEW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.138"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            271..768
                     /locus_tag="VVL53_002020"
     CDS             271..768
                     /locus_tag="VVL53_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4184122.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            768..2423
                     /gene="dnaX"
                     /locus_tag="VVL53_002021"
     CDS             768..2423
                     /gene="dnaX"
                     /locus_tag="VVL53_002021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU4184123.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2451..2645
                     /locus_tag="VVL53_002022"
     CDS             2451..2645
                     /locus_tag="VVL53_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU4184124.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
BASE COUNT          789 a          483 c          579 g          827 t
ORIGIN      
        1 ttattgatga tgtctaaaat ctggatatta gggtctttaa tgtctagtaa ttttgtgata
       61 aaatgtaatt gttccatatg aatctttcta atgagttgtt tggtcgcttt tcattatagg
      121 tcatatggga ctttttttct acaataaaat aggctccata atatctataa gggatttacc
      181 cactacaaat attatagagc caaaaatcct ttgtttacta aacaagggat ttttcttttg
      241 tctctgctcc ttttttgata taatagttct atgttaaaat cagaaaaaca atcacgttat
      301 caaatgttaa atgaagaatt gtccttccta ttggaaggcg aaaccaatgt tttggctaat
      361 ctttccaacg ccagtgctct cataaaatca cgttttccta ataccgtatt tgcaggcttt
      421 tatttgttcg atggaaagga attggtttta ggccccttcc aaggaggtgt ttcctgcatc
      481 cgtattgcac taggcaaggg tgtttgtggt gaggcagctc actttcagga aactgttatt
      541 gttggagatg tgacgaccta tctcaactat atttcttgtg atagtctagc taaaagtgaa
      601 attgtggtgc cgatgatgaa gaatggtcag ttacttggag ttctggatct ggattcttca
      661 gagattgagg attacgatgc tatggatcga gattatttgg aacaatttgt cgctattttg
      721 cttgaaaaga cagcatggga ctttacgatg tttgaggaaa aatcttaatg tatcaagcac
      781 tttatcgaaa atatagaagt caaaacttct cccagttagt tggtcaagaa gttgtggcta
      841 agactcttaa acaagcggtg gagcaagaga aaataagtca cgcttatctt ttttctggtc
      901 ctcgtggaac gggaaaaacc agtgttgcta aaatctttgc caaggctatg aactgtccca
      961 atcaagtggg tggcgaacct tgcaataact gctatatttg tcaagcagtg acggacggta
     1021 gtttagaaga tgtcattgaa atggatgcag cttctaataa tggggtagat gaaattcgcg
     1081 aaattcgtga taaatctacc tatgcgccta gccttgctcg ttataaggtt tatatcatag
     1141 atgaggttca catgctgtct acaggggctt ttaatgccct cctaaagacg ctggaagaac
     1201 caacacagaa tgtagtcttt attttggcca ctactgaatt gcacaagatt cctgctacta
     1261 ttctatcccg tgtgcaacgt tttgagttta aatcaattaa gacacaggat attaaggaac
     1321 atattcacta tatcttagaa aaagaaaata tcagttctga accagaggct gtggaaatca
     1381 ttgccagacg ggcggaaggt ggaatgcggg acgccttgtc tattttggat caagccctga
     1441 gtttgacaca gggaaatgag ctgacgactg ctatctctga agaaattact ggcaccatta
     1501 gcctatcagc cttggatgat tatgtggcgg ccttgtctca acaggatgtt cccaaagctt
     1561 tgtcttgctt gaatcttctt tttgacaatg gtaagagcat gactcgtttt gtgaccgatc
     1621 ttttgcacta tttaagagac ttgttaattg ttcaaacagg gggagaaaat actcatcata
     1681 gttcagtctt tgtagaaaat ttggcacttc ctcaaaaaaa tctgtttgaa atgattcgct
     1741 tagcaacagt gagtttagca gatattaagt ctagtttgca gcccaagatt tatgctgaaa
     1801 tgatgaccgt ccgtttggcg gaaatcaagc ccgaaccagc tctatcagga gcggttgaaa
     1861 atgaaattgc tacgctgaga caggaagttg cccgtctcaa acaagagctt tctaatgtag
     1921 gtgcggttcc taaacaagtt gcaccagctc ctagtcgacc agctacgggc aaaacagtct
     1981 atcgtgtcga tcgcaataaa gtgcaatcta tcttacaaga ggccgtcgaa aatcctgatt
     2041 tagcacgtca aaatctaatt cgtttgcaga atgcctgggg agaggtaatt gaaagtctag
     2101 gtgggccgga caaggctctg ctagttggtt ctcaaccggt tgctgccaat gaacaccatg
     2161 ctattcttgc ttttgagtct aacttcaatg ctggtcaaac tatgaaacga gacaatctca
     2221 ataccatgtt tggtaatatc ctcagtcagg cggcaggttt ttcacctgag attttagcta
     2281 tttccatgga ggaatggaaa gaagttcgcg cagccttttc agccaaagcc aaatcttctc
     2341 aaactgaaaa agaagtagaa gaaagcctga ttccagaagg atttgaattt ttggctgata
     2401 aagtgaaggt agaggaagac taaagaaaga tttcatgata caataagttt atgaataaac
     2461 aacaatttat tattatggcg ctgtttacag ctgctgagac ctattttttc aatgaagcct
     2521 ggatgactgg ccgctatatt atggcagcct tttgggcaat tttactcttt agaaatttcc
     2581 gagtcagtta tgtgatgggc aaaatcgttg atgtcatcga tcagcatttt aataggaaag
     2641 actagccctc agcttccaga caaaatcaaa gcctttta
//
LOCUS       DAVHEW010000139         2629 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVHEW010000139 DAVHEW010000000
VERSION     DAVHEW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.139"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..576
                     /locus_tag="VVL53_002023"
     CDS             <1..576
                     /locus_tag="VVL53_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4184125.1"
                     /translation="SKIPSRLYKIDTGKVLFDGVNINQIDKKILSQNLGVVPQDSFLL
                     NRSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRI
                     ALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIVAHRLSTIKDADVIF
                     VMKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            589..759
                     /locus_tag="VVL53_002024"
     CDS             589..759
                     /locus_tag="VVL53_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184126.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(1265..2611)
                     /gene="gdhA"
                     /locus_tag="VVL53_002025"
     CDS             complement(1265..2611)
                     /gene="gdhA"
                     /locus_tag="VVL53_002025"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU4184127.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          846 a          454 c          508 g          821 t
ORIGIN      
        1 tcaaaaatac catcaagatt atataagatt gatacaggaa aagttttatt tgatggtgta
       61 aatattaatc aaatagataa aaaaatatta agtcaaaatt taggagtagt tccacaggat
      121 tcatttttat tgaaccgaag tattcttgat aatataactt taaagcacga agttacttca
      181 caaaagatag aggaagtttg taaagcagtt caaatctatg atgaaatcat ggctatgccg
      241 atgaaattta atactatcat ctcagagatg gggtcaaata tttcaggtgg gcaaaggcaa
      301 cggatagcac tggcacgtgc attaataaat aatcctagta ttgtaatttt agatgaagca
      361 actagtgcat tagacactat taatgaggaa agaataacaa agtatataca aagtcagggc
      421 tgtactcaaa taattgtagc tcatagattg tcaacgatta aggatgcgga tgttattttt
      481 gtaatgaaag gtggtaagat tgttgagtca ggaaatcata agtacttaat ggatcttggt
      541 ggagagtact acagcttata tacaaaaagg aaatgaggtg taaagaaaat gaagaaagaa
      601 aatgaatatg taattttaac aacagcctca ctaggggtga tgattggaat agtgtttgca
      661 atttttttag attttccagt tgaatatggt atttctttag gcttgttgaa tggaatagta
      721 ttgggttcgc tgattgttta caaaaacaat aagaattaag cataattttt tgctgtaaac
      781 taaggagtag agatggctat agttgaaatt ataaatctaa caaaaagctt taaagatatt
      841 gaagttattc ataacactta aataatagag caactacagt agtagcttaa aaacatgatt
      901 aaatcgctat tcttaggagt agcggttttt ctttttgttt aatactcttt gaaaatctct
      961 tcaaaccacg tcagctttgc tttaccgtac tcaagtacag cctgcggctc gcttcctagt
     1021 ttgctctttg attttcattg agtataaaaa gggtcaagta agtatagtaa attgaaataa
     1081 gatatgaaca aatcgattag aaaagtcaaa ttaatttcta gaaatatgtt agaaattggt
     1141 ttgaattccg caatcaattt gttcagtttt tatttcattt cattttattt aattagattt
     1201 tccaattttt taattcaagc taaaaatccc caatcgtagt gattgaggat tgagtaaata
     1261 aatcttaaac aataccttgt gcaatcatgg catttgctac attttcaaag gcagcaatgt
     1321 tagctcctgc aaggtagtct ttatcaagac cgtatgtttc tgaagtcgtt ttagctgtgt
     1381 tgaagatgtt tgtcatgatg tctttgagac ggccatcaac ttcttcacga gtccatgaga
     1441 ggcgaagact gttttggctc atttcaagag ctgaaacggc tacaccacca gcgttggcag
     1501 cttttgcagg tccgtagaag ataccatttt ctttgtaaac tttgatggca tcaaggtcgc
     1561 tcggcatgtt ggcaccttca gatacacaga taacgccttg agcaaccaaa cgtttagctg
     1621 cttcaccgtt gatttcgttt tgagtggcac atggaagagc aatgtcatag tttccagcgt
     1681 aagtccatac agtaccttcg tggtaggttg cagttgcttt ttcagctgca tactcagtca
     1741 aacgagcacg acgtttttct ttaacatcaa ccaaaagatc gaagtcgata ccattttcat
     1801 cgatgacata accatttgag tcagaaacag aaataacagt tgcaccgagt tcagttgctt
     1861 tttgaagagc atattgagca acgttaccag aacctgaaat aacgactttc ttaccagcaa
     1921 agctgttacc gttagctttg agcatttctt cagtatagta aaccaaaccg taaccagttg
     1981 cttctggacg aatcaagcta ccaccaaatc caagaggttt accagtcaag acaccagcat
     2041 caaattggtt aagacgtttg tattgaccgt aaaggtaacc aatttcacgt ccaccaacac
     2101 cgatatcacc agcaggtacg tcaagtgatg gtccgatgtg tttttgcaat tcagtcatga
     2161 agctttggca gaagcgcatc acttcagcat ctgttttacc tttaggatcg aagtctgatc
     2221 cacctttacc tccaccgata ggaagtccag tcaagacatt tttaaagatt tgttcaaatc
     2281 cgaggaattt caagatccct tggtttacag ttgggtggaa acgaagtcca cctttgtatg
     2341 gtccaacagc tgagttgaat tgaacacggt aaccacggtt tacttgaatt tttccatcac
     2401 ggtcaaccca aggaacacgg aaagaaacca cgcgctcagg ctcagtaata cgtgccaaga
     2461 tattttcttc gatatactca gggtgttttt caaatacagg ttctaaagtg ttgaaaaatt
     2521 cttcaacagc ttggaggaat tcagcctcgt gcccgttacg agctttcaca gtttcaaaca
     2581 cgctttggat atattcttta gcagatgtca tatcgttctc cttttattg
//
LOCUS       DAVHEW010000140         2438 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVHEW010000140 DAVHEW010000000
VERSION     DAVHEW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.140"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(2..241)
                     /locus_tag="VVL53_002026"
     CDS             complement(2..241)
                     /locus_tag="VVL53_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU4184128.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(293..1729)
                     /locus_tag="VVL53_002027"
     CDS             complement(293..1729)
                     /locus_tag="VVL53_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU4184129.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(1919..2257)
                     /locus_tag="VVL53_002028"
     CDS             complement(1919..2257)
                     /locus_tag="VVL53_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU4184130.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVKEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT          827 a          512 c          295 g          804 t
ORIGIN      
        1 attatttata cttcaattta taaaataaaa ttattcctga gagtagaaat gaaacactat
       61 ttgctaaaat caaaggcaag tctcctatac gaataccatg agcaagccac aatgcaatac
      121 caataacttg cataacatac atacctagag caatagatcc tgtgtccttt gtcttaacta
      181 cacgaaaaac ttgtggtaaa aatgcaaatg ttgttaaaat tgctgcaata cttccaatca
      241 tatgtcacct caatatgcta aacaaactga gaataatctc agtttgttta tactattcta
      301 ctgattcacc gttagatgaa ataacttcct tataccagcc aaaagatttt ttcggggaac
      361 gattataact tcccttccca ttatcatctt tatctacata aataaagcca taacgtttcc
      421 gcatttcacc ggtaccagct gaaaccaaat caatacatcc ccatggagta taacccatta
      481 aatcaacacc atcttcaact acagcctttt tcatttcacg aatatgggca cctagatatt
      541 caattctata atcatcatgt accataccat ctgctgcaac ttgatctata gctccaaaac
      601 cattttcaac aataaagagt ggtaagtgat agtggtctgt aaaccaattt aacgcataac
      661 gcaaaccttc tggatcaatt tgccactccc attcagaagc cttaacataa ttatttttca
      721 ctaaatcttc tgtttcaaga taatcaaaat aaggattatt ttcacgatga gagtcgatag
      781 caaaggacat atagtaactg aaaccaatgt aatctacagt cccaccaagt aaatcttctt
      841 tatcctgggc agtaaaatca actgaaatac cttttcgttc ccaatacttg aaaatatgct
      901 caggatattt acctaaaaca tgcacatcag caaaataata acgcttctgc atagctttca
      961 ttgccattaa gatatcctta ggattgcaag taactggata aattggacac atcgcaatca
     1021 tacaacctat ttgaaaatct ggattaatct catgaccaat ttttacagct cgtgcagaag
     1081 caactaattc gtaatgtgct gcttgataca taattgcttc tctattatca ccttcctcat
     1141 atacaatacc tgagttagta aatggtgcaa aatcttcctg ataattcgct tgattattga
     1201 tttcattgaa agtcatccaa tatttaacct tatctttgta acgtttaaat acgacttctg
     1261 caaaacgagc aaagaaatca atcaatttcc tatttttcca accaccatat tcggtcacta
     1321 agtgataagg catttcaaaa tgagatagag tgatgacagg ttcaatacca ttctttaagc
     1381 attcatcaaa aagattatca taaaactgta atccttcttc attcggctct aactcatcac
     1441 cttttggaaa gatacgtgtc catgcaatag aggtacggaa gcacttgaat cccatttcag
     1501 caaaaagtgc tatatcttct ttataacggt gataaaaatc tatcgcctca tgatttggat
     1561 aatatttacc ctctaaaact cccaaagtaa tttcacgagc tactccatga cgaccagcag
     1621 tcataacatc agcaacacta attcccttgc caccttcttg ccatccacct tcaagttgat
     1681 gagcagcaac agcaccaccc cataaaaatc catctttaaa agtagtcatc ttttttcctc
     1741 ctgactttga tactcttatt ataaacctta aaccaaaaga tgaaaacgca ttctttttcc
     1801 ttattgttaa ggaaagaagt aatttttaat ggaaatagaa caatatcttc ttgtattctc
     1861 gtaatgatat ctttacgatt ttcaatactt tcaaactaca aaaactctca caataattct
     1921 aattccctgt gtctataaac gacttatcgc tttctggcat cccagaatca tcttctatat
     1981 aacgttcaac ttgcatctgc aagtgatatt tttttcttaa atctaagatt ttctgcattg
     2041 tctttgattg ataatgttta tctaaagttt cttgatttat ccactgatca ataaggagaa
     2101 tagttccctc tttttcaatt ggtaaaaaat attcgtattt caagttacct ttttgatttc
     2161 taatttcttt aacaaggcca ctatcaagca tttctcttgc aaactttatt gcactatctc
     2221 catcaccttt ataatataca tgaatagtca atgtcatctt atatcctcca aaatcatcct
     2281 tcaattttaa aaaaacaagt ttagatgagg atctaaactt gttttttatg aactaattat
     2341 ctaacgtttc gccattactt tcaatcactt ctttatacca ataaaatgat tttttcttat
     2401 agcgatttat agtcaattga aacaagagca ggacaaaa
//
LOCUS       DAVHEW010000141         2419 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVHEW010000141 DAVHEW010000000
VERSION     DAVHEW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.141"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..459)
                     /locus_tag="VVL53_002029"
     CDS             complement(<1..459)
                     /locus_tag="VVL53_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4184131.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHK"
     gene            581..1327
                     /locus_tag="VVL53_002030"
     CDS             581..1327
                     /locus_tag="VVL53_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU4184132.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1340..2320
                     /locus_tag="VVL53_002031"
     CDS             1340..2320
                     /locus_tag="VVL53_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU4184133.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          863 a          291 c          459 g          806 t
ORIGIN      
        1 tttatgtttt atagaattat aatgttttag gtctgtgtag ataaaatcca catattgaat
       61 taaatcaata aatttttcat gatcaacaaa ggcagtagtt tcaatggcag tgtgtatgtg
      121 atgttcttta gctgatttta agatggcttt agcaaattca aactgagcaa atatttcacc
      181 tcctgataaa gttaaacctc caccggattc ttcgtaaaat tctttgtctt ttaatacctc
      241 tgtaataatt tcttctacag tcttttcttc accgactaag gtgaatttct ctcgttgagc
      301 atctttcatt ttttcaggtt tcattctttg agattcagga ttagaacacc atggacagcg
      361 cagaggacat ccttttaaaa aaacagttgt acgaataccc ggaccgtcat gaattgaaaa
      421 gtgttgaata ttaaaaataa ttcctttaga tatttccata agagttcctc ctcttcataa
      481 gtctatccta tcacaaaaac gaaagaaaaa caattacgaa cgaaaggtta atttgttgtt
      541 ttttgctttt tattttggta gaataaaacg gaggtgtaaa atgaaaagat tggaacaaat
      601 tattaaatta gtatcagaat atgaaaagat cgacgttaat acattatcgg aaaaattaaa
      661 tgtatcgaaa gtaacgatta gaaaagattt agataaatta gagtcaaaag gtttattaca
      721 cagagagcat ggatatgctg tattaaatag tggagatgac ttaaatgtac gtttgtcaat
      781 taattatgaa actaagagaa aaattgttca ggaagcagta aaattggtgt cagataatga
      841 aacaataatg atagaatctg gatcgacctg tgctttactt gctgaggaaa tttgcaagca
      901 aaaaagaaat gttacgattg taacaaattc gttttttata gcaaattttg tgagagctta
      961 tgattcatgt cgtgttattg ttcttggtgg tgagtttcag aaagattcac aggtgactgt
     1021 aggaccttta ttaaaagaaa tgatacagac ttttcatgtg tgtcaagctt ttgttgggac
     1081 agatggttac gataaagaga tgggctttac cggaaaagat ttaatgcgca gtgaggtagt
     1141 tcaatatatt tcagcagtgt cggataaagt cattgtccta actgactcaa gtaaatttga
     1201 taaaagaggt acagtaagaa gatttgcttt aagtcaagtc tatgaagtaa taacagacga
     1261 aaaactttct aaacaaaata tagctacatt agaaaatgct gggataatgg ttaaggtagt
     1321 ttcgtaagag gttaagtgta tgaatcaaga taggaataaa ctgctttcta aaattgctta
     1381 tctgtattat attgaaaact taaatcagtc acaaatagca gcaaaattag gaatttatag
     1441 aacctctatt agtagaatgt taacagaagc aaggaatgta ggaattgtta aaattgaaat
     1501 agagaatttt gataccaata tgtttaagtt ggaaaattat gtaaaagaaa aatacagttt
     1561 ggaaagttta gaaattattc caaatgaatt tgatgatact ccaacaattt tatctgaaag
     1621 aatttctcaa gttgcagcag gcgtccttag gaatctaatt gatgataata tgaaaattgg
     1681 cttttcttgg gggaaaagtt taagtaattt agtagattta attcacagta aaagtgtccg
     1741 aaatgttcac ttctatcctc tagcaggtgg tcctagtcac atacacgcta aataccatgt
     1801 gaatacactg atttatgaaa tgtctagaaa atttcatgga gagtgtacat ttatgaatgc
     1861 aacgattgtg caagaaaata aattgttagc agatggtatt ttgcaatcaa gatattttga
     1921 aaatttgaaa aatagttgga aagatttaga tatagctgta gtcggaattg gtgattttag
     1981 caataaaggg aaacatcaat ggttagacat gcttacagag gatgacttta aagaattgac
     2041 caaagttaaa actgtaggag aaatttgttg tcgatttttt gattcaaaag gtaaagaagt
     2101 ctatgaaaac ttacaggaaa gaacgatagc aatctctttg gaagatttaa aaaatattcc
     2161 tcaaagttta gctgttgctt acggtgatac gaaagtatct tcgattcttt ctgtcttgcg
     2221 tgctaattta gtaaatcatt tgattacaga caaaaataca attttaaaag ttttggaaga
     2281 agatggggat ttgactttta gagagattct aggtgagtga aaatgataga ctgattcagt
     2341 ttatcgtttt tctttttagt tgattgcaca tttgtgctta tataaacaaa aatagtttat
     2401 ctgttgtttt tggattgac
//
LOCUS       DAVHEW010000142         2349 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVHEW010000142 DAVHEW010000000
VERSION     DAVHEW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.142"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..155)
                     /locus_tag="VVL53_002032"
     CDS             complement(<1..155)
                     /locus_tag="VVL53_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4184134.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSS
                     GKFLDKH"
     gene            complement(121..1086)
                     /locus_tag="VVL53_002033"
     CDS             complement(121..1086)
                     /locus_tag="VVL53_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4184135.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1204..1617)
                     /locus_tag="VVL53_002034"
     CDS             complement(1204..1617)
                     /locus_tag="VVL53_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU4184136.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1614..1850)
                     /locus_tag="VVL53_002035"
     CDS             complement(1614..1850)
                     /locus_tag="VVL53_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU4184137.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(1910..>2349)
                     /locus_tag="VVL53_002036"
     CDS             complement(1910..>2349)
                     /locus_tag="VVL53_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4184138.1"
                     /translation="RVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTH
                     GKVWISDNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYIL
                     PPLVLQNEFADFVALVDKSQLAIQKSLEELETLKKSLMQEYFG"
BASE COUNT          696 a          451 c          350 g          852 t
ORIGIN      
        1 tcatgtttat caagaaattt tccacttgaa aatttaaaac actgttgtcc cgtttttact
       61 gcaaatttct tgttattatt taaaggatcc ccaaacatct cgttaaatcg ggatttgctt
      121 ttaagagatg aatttttggt gtgaatgctt gacattattt tgattgacca tggcataggc
      181 cagggttgtg tcaatcttgc tatgacctag cagtttttgg acttgttcga taggcatacc
      241 tttatcaatt gccttagtcg ctaaagttct tctgaactta tgtgggtgaa ccttttgtat
      301 gccgagtttg tctcctaact ttcttaagcg aatctctaca ccagcaattc caagtctctg
      361 ggcttttcca actagcgtta caaaaagagc agggtgacta tcttttctgt cgttaagata
      421 atttcttaaa tgaattttcg tccgagcgtc aaaatatact ggtctctcct tctttccttt
      481 accaaagaca acacactctc tgttttcaaa atcaatatct gaacgattca actgtacaag
      541 ctcccctaca cgcatacctg tcgatgctag taggtctatt attgccaaat ctctcaaatt
      601 ttcacagtta tcacgcataa tttccaaatg ttcatcagta taagtttcct tcacattttg
      661 ctcagtctta attttcttta tccgtcgaat gggattttta atgatatatt cctcttgctc
      721 aagccaagca aaaaaagaag acaatatacg cctaatattg tctaaatttg ccttactaca
      781 atccttttca gactggtaat ttgctaaata actccttaaa tcatctgttg tgagttgtgt
      841 cacagactct ttaatagcat taaacaagtt ttcaatcgtc ctctggtaat aacgaattgt
      901 ttttgagcta cagccctcta cctgcttggc ggtaataaat agttgcaaca ggttattatt
      961 cgaatacttc tgctgctttt ttccgtctac tagatgatgt tctaaactct ctctcaacgc
     1021 tagtaattgt tcattattta aaagaggtaa catttcttgc atgataattg taacttttga
     1081 ttccataatt tctcactttc ttattcatca ataaaaagag acaatatcag tttctgcatt
     1141 acagaattct aatattgtct gttcatttat tttcttgtgc tcattcaaat ccagcatact
     1201 aggttacttt ttgacctttt ctctaacaga atgttcagaa acccatttgg agtagcttgt
     1261 cattttttca tcagttaaag cttctactgc gatggttaca cacattttta ctgcttcttc
     1321 taccgttaca gaaaaacgat agccgtttaa ttgtaaaaat ttgaccaaaa cgaagaaagc
     1381 agttctttta ttagcattag caaaaacatg cttctttatc aattggacaa atagtatcgt
     1441 tgctttatca aaaattgttg gataaagaga cttcccaaag acaaattgtt ctggtaagtt
     1501 cacaatcata tttaaggcag aagaactaac tgtttgaatt ttctcatttg gagaataccg
     1561 ttgaattgct aaagcattta ttttttcaat ttgcttttct gtcagataga ttgtcatttt
     1621 tccaccaaag ctttgaaaac atcgtcgtat tcgtcaaata ttttatctaa atttaagtca
     1681 aaggcatcat cagatacata agaaacctgc tgccccaatt cttcaggagt aaagataatc
     1741 tcaccacttg gtaacaattt cgcttcgtat ttaacaccac ttggaatatt aaattcactt
     1801 ggaatcgtaa tggtgattga atttccttgt tttcttgttt ttactaccat ctgatttcct
     1861 cctttgaagt attataacaa atcattaccg taattacaat ggcagaatat cagccaaaat
     1921 actcctgcat cagagatttc ttcaaagttt caagttcttc cagagatttt tggattgcca
     1981 attgtgattt gtcgaccagg gctacaaagt ctgcgaactc attttggagg actagagggg
     2041 ggaggatata tttctgattt tctaatggtt tttgagttat ctttggttga cctgagaaat
     2101 cagcaaattt actaaaatca attactttca ttaattctaa taaaaataca agattgaaat
     2161 cggaattctt aaattctttt atatatatag cattgtcact tatccatacc ttaccgtggg
     2221 tagttcttac attaccacaa tatgcaccta cacgaccaat gatgatagta ggattatcaa
     2281 ttaaatattt tcgacttttc caagcaatgc catttccacc ataggcagga taaccttcaa
     2341 aaactctat
//
LOCUS       DAVHEW010000143         2289 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVHEW010000143 DAVHEW010000000
VERSION     DAVHEW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.143"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(1..175)
                     /locus_tag="VVL53_002037"
                     /pseudo
     CDS             complement(1..175)
                     /locus_tag="VVL53_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(270..455)
                     /locus_tag="VVL53_002038"
                     /pseudo
     CDS             complement(270..455)
                     /locus_tag="VVL53_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            483..710
                     /locus_tag="VVL53_002039"
     CDS             483..710
                     /locus_tag="VVL53_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4184139.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            786..1232
                     /locus_tag="VVL53_002040"
                     /pseudo
     CDS             786..1232
                     /locus_tag="VVL53_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1303..1461
                     /locus_tag="VVL53_002041"
     CDS             1303..1461
                     /locus_tag="VVL53_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU4184140.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1554..>2289
                     /locus_tag="VVL53_002042"
     CDS             join(1554..1922,1924..>2289)
                     /locus_tag="VVL53_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU4184141.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAAVFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKELANYSGKKKFHAMKAQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIRQAGKILADSGYQGLMKIYPQAQTSRKSSKLKPLTIEDKVYNHA
                     LSKERSKVENIFAKVKTFKMISTTYRN"
BASE COUNT          733 a          410 c          479 g          667 t
ORIGIN      
        1 ttgacaatct tctctttatc tttgaggaag gttttagagg atgaacttga ttcagattgt
       61 cctcaatgag tccgaaaaat ttgtcaggct ccttattctg aaagtgaaaa agcaagagtt
      121 gatagagatt atagtggtgt ttcaagtctt ctgaatagct caaaagttta tctatagtag
      181 attgaaacta gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgactgt
      241 cctgaatgat ttgtcctgtt attatttcat tttactataa atccacgttt acgaatctct
      301 ttccacactt gttcaatggg gttcatctct ggtgtgtatg gaggaataaa tgcaaaacca
      361 atattagtcg gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt
      421 aaaagataat catctggata agcttgtgaa agctcctatt cctaaagccc ctttataacc
      481 tcttgcgaga gagactattg actcagccct tacttcatgc ggatgaaact tcttatcggg
      541 ttctagagag tcatagccat ctgacctact attggacctt tttgtctggg aaagttgaga
      601 atcaagcaat cacgctgtac catcatgatc agagtcggag tggttcggta gtacaagaat
      661 tcctaggaga ttattctggc tatgttcatt gtgatatgtt gcggcagtaa cttaggactt
      721 tagtcctcta gttctgccta tgcgatagca gtccaaggtt taggagcaag gcgacgctaa
      781 gcttggtaaa ctgcgaaccg ctagaagctt atcgtcaact ggaagaagct gaacttgttg
      841 gatgttgggc gcatgtgaga aggaaatttt ttgaagcgac ccccaagcaa gcagataaat
      901 catccttagg agctaaaggt ttagcttatt gtgatcagtt attttccttg gaaatagact
      961 gggaggcttt gccagctgat gaacgactac agaaacgtca agaacatctc cagcccctaa
     1021 tggaagactt ctttgcttgg tgccgccgtc agtcagtttt agcaggttca aaactaggaa
     1081 gggcaattga atacagcctc aagtatgaag aaacctttaa gactattttg aaagacggac
     1141 atctggtcct ttccaataat ctagctgaac gcgccattaa atcattggtt atgggacgga
     1201 gtaaaagagt ccagtggact cttttagcct aagctcagtt taaaaaagcg agggtggtta
     1261 ttttctcaaa gttttgaagg agctaaagca agagctatta ttatgagttt gttggaaaca
     1321 gctaaacgtc atcaattaaa tagcgagaaa tatctatcct atcttctaga atgtcttcca
     1381 aacgaggaaa ctctcgtaaa caaagaggtt ttagaggctt atttaccatg gactaaagtt
     1441 gtacaagaaa agtgcaaata agaaatctcc agattaggaa ctatccgtga gttctccagt
     1501 ctggagattt ttcaatagac ttcctgcgaa acaaaatatg gtataatagt tctatgaatg
     1561 atgaagcaag taaacaacta accgatgcac gatttaagcg tcttgttggt gttcaacgca
     1621 cgacttttga agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag
     1681 gtggacgaaa acctaaatta agtctagaag accttcttat ggccactctt caatatgtgc
     1741 gagaatatcg aacttatgaa caaattgcgg ctgtttttgg tattcacgaa agcaacttaa
     1801 tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggtgttacg atttcaagaa
     1861 ctcctctcag ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc
     1921 ctaaaaaaag aattagcgaa ttattctggt aaaaagaaat ttcacgctat gaaggctcaa
     1981 gcgattgtca caagtcaagg gagaattgtt tctttggata tcactgtgaa ctattgtcat
     2041 gatatgaagt tgttcaaaat gagtcgcaga aatatcagac aagctggtaa aatcttggct
     2101 gacagtggtt atcaagggct catgaagata tatcctcaag cacaaacttc acgtaaatcc
     2161 agcaaactca aaccgctaac aattgaagat aaagtctata accatgcgct atctaaggag
     2221 agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgat ttcaacaacc
     2281 tatcgaaat
//
LOCUS       DAVHEW010000144         2202 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVHEW010000144 DAVHEW010000000
VERSION     DAVHEW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.144"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..720
                     /locus_tag="VVL53_002043"
     CDS             <1..720
                     /locus_tag="VVL53_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4184142.1"
                     /translation="NDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNK
                     GSIKIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKL
                     AGCDLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPD
                     NEFELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRY
                     KSLQEMFNSANEWRVSNERVL"
     gene            704..2202
                     /locus_tag="VVL53_002044"
                     /pseudo
     CDS             704..2202
                     /locus_tag="VVL53_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          826 a          294 c          420 g          662 t
ORIGIN      
        1 aatgataaag ctgtcattga aaatttatcc tttaatttag aagaaggaaa gtcctacgca
       61 cttgtcggtt catctggatc aggcaaatca acagtagcaa aacttatatc aggtttttac
      121 aatgttaata aaggaagcat aaagataggc gggatagcaa taagtgaata ttctgacgaa
      181 gccttaatta aagccatttc ctttgttttt caagattcaa aattattcaa gaagagcatt
      241 tatgataatg tagcgttagc taataaagat gcgacgaaag atgacgttat gagagcctta
      301 aaattagcag gatgcgattt aatattagac aaattcccag aaagagaaaa tacaatcata
      361 ggctcaaaag gtgtttattt atccggtgga gaaaaacaaa gaattgcaat tgctagagca
      421 attttaaagg attccaaaat tattattatg gatgaagcat cagcatctat tgacccagat
      481 aacgagtttg aattgcaaaa agcttttaaa aatcttatga aggataaaac agttatcatg
      541 attgcacaca ggctatctac aattaaagac cttgatgaaa ttattgtcat ggatagtgga
      601 aaaattatag aaagagggtc tgacaaagaa ttaatgtcaa aagatacaag gtataagagc
      661 ctgcaagaga tgtttaacag tgcgaatgaa tggagggttt caaatgaaag agttttataa
      721 aaaaagattt gctcttacag atggaggagc aagaaattta agtaaagcaa cactggcttc
      781 atttttcgtt tattgtataa acatgcttcc tgccatatta cttatgattt ttgctcagga
      841 agttttggaa aatatgggca aaagcaatgg cttttatata gtattctcag ttttgatttt
      901 gatagcaatg tatattttgc tttctatcga atacgataaa ttatataaca caacctatca
      961 agaaagtgca gatttaagaa taaggacagc ggagaattta tcaaaattac ctctatctta
     1021 cttttctaaa catgacattt ccgacatttc acaaacaatc atggctgata ttgaaggcat
     1081 agagcatgca atgagccact caataccaaa ggtgggcggc atggtactgt ttttcccatt
     1141 aatatctgta atgatgctag cgggcaatgt caagatgggt ttagctgtaa ttattccatc
     1201 tattttaagc tttatattta tacctttatc taaaaaatat caggttaatg gacagaatag
     1261 atattatgat gtcttaagaa aaaactcaga aagctttcaa gaaaatatcg aaatgcaaat
     1321 ggagattaaa gcatataatt tatcgaagga tattaaagat gacttatata aaaaatggaa
     1381 gatagtgaga aagtacactt aaaggcggaa gtaactacaa ttttaacttt gtctatatct
     1441 tcaatattta gctttatatc tcttgctgtt gtgatatttg tcggcgtaaa tctaattatt
     1501 aataaagaga taaattctct ctaccttata ggatatttac tagctgctat gaagataaaa
     1561 gactctttag atgcatctaa agagggcttg atggaaatat tttatttatc gcccaaaata
     1621 gaaagattaa aagaaattca aaatcaagat ttacaagaag gcgatgacta tagcttaaaa
     1681 aaatttgata ttgatctaaa agatgttgag tttgcctaca ataaagacgc aaaagtttta
     1741 aatggtgtaa gttttaaagc taagcaggga gaggtcactg ctttggtagg tgcaagtggc
     1801 tgcggtaaaa caactatctt gaaacttata tcaagacttt atgattatga caagggacaa
     1861 atcttaatcg atggcaaaga tataaaggaa atatcaacag aatccctttt tgataaggtg
     1921 tctattgttt tccaagatgt ggttctcttt aatcaaagcg ttatggaaaa tattagaatc
     1981 ggtaagcaag atgcaagtga cgaagaggtt aaaagagcag caaaacttgc aaattgcaca
     2041 gattttatag aaaaaatgga taaaggtttc gatacagtta ttggtgaaaa cggagctgag
     2101 ctatcaggag gagaaagaca aagattatca atagccagag ccttcttaaa agatgcgccg
     2161 atattgatct tagatgagat aacagcaagc cttgatgtta ac
//
LOCUS       DAVHEW010000145         2136 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVHEW010000145 DAVHEW010000000
VERSION     DAVHEW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.145"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            62..850
                     /gene="codY"
                     /locus_tag="VVL53_002045"
     CDS             62..850
                     /gene="codY"
                     /locus_tag="VVL53_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU4184143.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PIESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            850..1425
                     /locus_tag="VVL53_002046"
     CDS             850..1425
                     /locus_tag="VVL53_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU4184144.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELFE"
     gene            1507..1592
                     /locus_tag="VVL53_002047"
     tRNA            1507..1592
                     /locus_tag="VVL53_002047"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1541..1543,aa:Leu,seq:aag)
     gene            complement(1715..>2136)
                     /locus_tag="VVL53_002048"
     CDS             complement(1715..>2136)
                     /locus_tag="VVL53_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4184145.1"
                     /translation="SDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHF
                     QNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIK
                     LIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
BASE COUNT          640 a          345 c          486 g          665 t
ORIGIN      
        1 taattattga aaaagagacg tttttatgtt ataatgatag cgattaaatt cgaggtgaaa
       61 aatggcacat ttattagaaa aaactagaaa aattacatca attttgaaac gctcagagga
      121 gcagttgcag gatgagctcc cttacaacgc tattacgcgt cagttagcgg atattattca
      181 ttgcaatgcc tgcattatca atagtaaggg acgtctgctt ggctatttta tgcgttataa
      241 aacaaataca gatcgcgtag agcaattctt ccaaactaag attttcccag atgactacgt
      301 tcaaggggct aatatgattt acgaaacaga agcaaacttg cctgttgagc atgatatgag
      361 tattttccct attgagagta gagatgattt tccagatggc ttgacgacta ttgcaccgat
      421 tcatgtatcg gggattcgcc ttggttcttt gattatttgg cgtaatgata aaaaattcga
      481 agatgaggac ttggttcttg ttgagattgc cagtaccgtt gttgggattc agcttcttaa
      541 cttccaacgt gaagaagatg agaaaaatat tcgtcgtcgt actgctgtca ccatggcggt
      601 caataccctt tcttactccg aactccgtgc tgtttcagca attttagggg aattaaatgg
      661 aaatgaaggg aagttgactg cgtcagtgat tgcagatcgt atcggaatca ctcgctctgt
      721 gattgtcaat gctcttcgta aacttgagtc tgcggggatt attgaaagtc gctcacttgg
      781 aatgaaggga acctatctta aggtcttgat ttcagatatt tttgaagaag tgaagaaaag
      841 agattactaa tgacaaaggc tttaatttcg attgattata cagaagattt tgttgctgat
      901 agtgggaaac taacagcagg tgctccagct caggcgattt cggatgccat cagcaaggta
      961 actcgattag cttttgaacg aggagattat atcttcttta ctattgatgc tcatgaagaa
     1021 aacgattgtt tccatccgga aagtaagtta tttcctcctc ataatctgat tgggacgagt
     1081 ggacggaatt tatatggaga tttggggatc ttttatcaag agcatggttc agacagtcgt
     1141 gtcttttgga tggataaacg ccattactca gctttttcag ggactgacct agatattcgt
     1201 ttgagagagc gtagagtgtc taccgttatc ttaacaggtg tcttgacgga tatctgtgtc
     1261 ctacatacag ctatagatgc ctataatcta ggatatgaca tcgagattgt taaaccagct
     1321 gttgcttcca tctggcctga aaatcatcaa tttgccctag gtcatttcaa aaatacactc
     1381 ggagctaagt tagtagatga aaatctaaat gaactttttg agtaacctgg ttggtgaaaa
     1441 tgaaaaaatt tgaaaaaaag tgttgacaag catcccaaaa gttgatatac tagtaaagta
     1501 atcgacgcgg ggatggcgga attggcagac gcgcaggact aaggatcctg tgaccgcttt
     1561 aggtcgtgag ggttcaagtc cctctctccg cataggaaaa gagctagcta aagctagctc
     1621 ttttgtattt ctctaaaaaa ttagaatcca gtcaaatgta gtggagaatc agcgaaaata
     1681 ggctctttgt caactgtagt gggttgaaga aaagctaagc ttgagaaagg acaaatttcg
     1741 tcctttcttt tttgatgttc agagcgataa aaatccgttt tttgaagttt tcaaagtttc
     1801 gaaaaccaaa ggcattgcgt ttgataagtt tgatgagatt attggtcgct tccaatttgg
     1861 cgtttgaata gggtagttga agggcgttga cgattttctc tttgttcttt agaaaggttt
     1921 taaagacagt ctgaaaaaga ggatgaacct gcttcagatt gtcctcaatg agtccgaaaa
     1981 atttctcagg gtctttgttc tgaaagtgaa aaagtaagag ttgatagatc tgatagtggt
     2041 gtttcaagtc ttctgaatag cttaaaatct tgtcaagaat ttctttattt gttaagtgca
     2101 tgcgaaaagt agggcgataa aaacgtttat cgctga
//
LOCUS       DAVHEW010000146         2133 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVHEW010000146 DAVHEW010000000
VERSION     DAVHEW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.146"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            75..1136
                     /gene="dinB"
                     /locus_tag="VVL53_002049"
     CDS             75..1136
                     /gene="dinB"
                     /locus_tag="VVL53_002049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU4184146.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(1283..2128)
                     /locus_tag="VVL53_002050"
     CDS             complement(1283..2128)
                     /locus_tag="VVL53_002050"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU4184147.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          715 a          429 c          434 g          555 t
ORIGIN      
        1 acactgtttt gaggttgcag ataaagctga cgcggtttga agagattttc gaagagtata
       61 aaaaggtgct aggcatgttg atttttcctt tgttaaatga tttgtcaaga aaaatcatcc
      121 atattgacat ggatgccttt tttgctgcag tggaaatcag ggataatcct aaactcagag
      181 gaaaacctgt cattattgga agcgaccctc ggcaaacagg tggacgggga gtcgtttcta
      241 cctgtagtta tgaggcaaga gcttttggtg tccattctgc catgagttcc aaggaagctt
      301 atgaacgttg tccccaggct gtctttatct cagggaatta tgagaaatac aagtctgtgg
      361 gactccagat tcgagctatt tttaagcgct atacagattt gattgaaccc atgagcattg
      421 acgaagccta tttggatgtg acagaaaata aactcggtat caagtcagcg gtcaaaattg
      481 ctcgcctcat tcaaaaagat atctggcaag aactccatct aactgcttcc gcaggcgttt
      541 cttacaacaa attcttagct aaaatggcga gtgattatca aaaaccacat ggtttgacag
      601 tgattctacc tgaacaggct gaggattttc tcaaacaaat ggatatttcc aaatttcatg
      661 gagtaggaaa aaagacagta gaacgtcttc atcaaatggg cgtttttact ggtgctgatt
      721 tacttgaagt tcctgaggta accctaatag accgttttgg tagactaggc tatgatctgt
      781 atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc caatcgcatc cgtaaatcaa
      841 tcggcaagga gaaaacctac gggaagattc tccttgctga ggaagatatc aaaaaagagc
      901 tgactcttct atcagaaaaa gtcgctctca atctacatca acaagaaaaa gctggaaaaa
      961 ttgtcatttt gaaaatccgc tacgaggact tttcaactct taccaaacga aaaagtcttg
     1021 ctcaaaaaac acaagatgct agtcagataa gccaaatagc cctgcaactc tatgaagaat
     1081 taagcaagaa agaaagaggt gtccgcctat tggggattac catgactgga ttttaaagct
     1141 caatgaaaat caaagagcaa actaggaggc tagccgcagg ctgctcaaaa cactgttttg
     1201 aggttgcaga taaagctgac gcggtttgaa gagattttcg aagagtataa aaccttgaag
     1261 agttgcccct tcaaggtttt tcttatacaa ataaacggac aaaactgtaa agtagcaaaa
     1321 cactaccaag cacgatacgg tatttaccaa aaagggtgaa gtcgtgtttt ttcacatagc
     1381 tggtcaagaa gcgaatagcc accatgctga ccgcaaaagc tactcccatc gcgaccaaga
     1441 gcaaaaacaa ttgcccaaag ctcaagagtt ctccggcttt cacaaatttg aaaatcttta
     1501 aggcactagc tccaaacata acaggaatcc caagatagaa ggtaaattct gtcacaactg
     1561 aacgactggt tccatttaac aaaccaccga caatcgttgc acctgaacgg ctagtccctg
     1621 gtaaaagagc aagaacttgg aagagtccga tatagaaagc ggtcgtataa ggaagcttgt
     1681 ccaactctgt tacacttggc tcgatagcac gcgctttatt gcgcttttcc aaatagatga
     1741 aggcaacccc gtagataatc aacatgagag caactgaaac catgttatgg aagtgggtat
     1801 caaaccaatc atcaaattta aagacaccaa gtaaaggtaa agtagcaatc aagaccttca
     1861 accatagtct ccaagtctta cgaacttcct gtttgtcctt ggtcggttta aaaggattga
     1921 gcttgttaaa ataaatcacc ataactgcta aaatagcacc aagctgaatc acgacattaa
     1981 acatggacat aaaggcttca ttttgatttt ggtattggat gaattcctct gctaaaatca
     2041 agtgacctgt actggaaatc ggcaaccatt ccgtaattcc ttcaacaatt ccgaagaaga
     2101 tagattttaa aatttcaata agatacatag att
//
LOCUS       DAVHEW010000147         2018 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVHEW010000147 DAVHEW010000000
VERSION     DAVHEW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.147"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..643
                     /locus_tag="VVL53_002051"
     CDS             <1..643
                     /locus_tag="VVL53_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4184148.1"
                     /translation="NVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVE
                     KEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSL
                     PLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLS
                     EIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            667..>2018
                     /locus_tag="VVL53_002052"
     CDS             667..>2018
                     /locus_tag="VVL53_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4184149.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     "
BASE COUNT          753 a          249 c          380 g          636 t
ORIGIN      
        1 aaatgttact attaaaaaag gagaaactgt tgctattgtt ggagaatcag gttcaggtaa
       61 gagtacatta gctaaaattt tattaggttt attagaacct aatattggtt caatagaagt
      121 tgatggagta gaaaaagaag aaattggtca aacattgtat agaaagattt ttggagcagt
      181 gttacaaaat tcaaccctaa gttatggtac cttaagagag aatttgacat ttggacactt
      241 tgtttcagat gaagaattaa tgacaaatct aaattcaatt ggtcttagca atgtagttaa
      301 atctttacct cttggattag agacaatcat cgctgaagaa ggtaataact tttctggagg
      361 gcagcagcaa atgatacttt tagctcgttg tcttttgtcg aaaccttcgg tagttgtttt
      421 ggacgaagca acaagtagtt tagataattt atctcaacaa attacaactt cttacttaag
      481 tgaaatcggt accactaaga ttttaattgc ccatcgacta gatactatca agtctgcaga
      541 taagatctta gtaatgcata atggtgaaat tgtagagatt gggacccata gagaacttct
      601 tgaactagga ggcatttata agcaattgta ttcaaataat tagtttttga ttaaaagggt
      661 aaatttatga agattatgaa aaaaaaatat tggactttag cgatattatt cttttgtttg
      721 ttcaataatt ctgttactgc tcaagaaata cctaaaaatc ttgatggcaa tataactcac
      781 actcagacta gcgaaagttt ttctgaatct gatgaaaaac aggttgacta ttctaataaa
      841 aatcaagaag aagtagacca aaataaattt cgtattcaaa tcgataagac agaattattt
      901 gtaacaacag ataaacattt agaaaaaaac tgttgtaaat tggaacttga accacaaata
      961 aataacgata ttgttaactc tgaaagtaat aatttactag gcgaagataa tttagataat
     1021 aaaattaagg aaaatgtttc tcatctagat aatagaggag gaaatataga gcatgacaaa
     1081 gataacttag aatcgtcgat tgtaagaaaa tatgaatggg atatagataa agttactggt
     1141 ggaggcgaaa gttataaatt atattctaaa agtaattcta aagtttcaat tgctatttta
     1201 gattcaggag tcgatttaca aaatactgga ttactgaaaa atctttcaaa tcactcaaaa
     1261 aactatgtcc ccaataaagg atatttagga aaagaggagg gagaggaagg aataatatca
     1321 gatattcaag atagattagg tcatggtacg gctgttgtag ctcaaattgt aggggatgac
     1381 aatattaatg gagtaaatcc tcacgttaat attaacgtct atagaatatt tggtaagtcg
     1441 tcagctagtc cagattggat tgtaaaagca atttttgatg ctgtagatga tggcaatgat
     1501 attatcaatc ttagtactgg acaatattta atgattgatg gagaatatga ggacggaaca
     1561 aatgattttg aaacattttt gaagtataaa aaggctattg attacgcgaa tcaaaaagga
     1621 gtaattatag tagctgcatt agggaatgac tccctaaatg tatcaaatca gtcagattta
     1681 ttgaaactta ttagttcacg caaaaaagta agaaaaccag gattagtagt tgatgttcca
     1741 agttatttct catctacaat ttcggtcgga ggcatagatc gcttaggtaa tttatcagat
     1801 tttagcaata aaggggattc tgatgcaata tatgcgcctg caggctcaac attatctctt
     1861 tcagaattag gacttaataa ctttattaat gcagaaaaat ataaagaaga ttggattttt
     1921 tcggcaacac taggaggata tacgtatctt tatggaaact catttgctgc tcctaaagtt
     1981 tctggtgcga ttgcaatgat tattgataaa tacaaatt
//
LOCUS       DAVHEW010000148         2017 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVHEW010000148 DAVHEW010000000
VERSION     DAVHEW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.148"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            29..1054
                     /locus_tag="VVL53_002053"
     CDS             29..1054
                     /locus_tag="VVL53_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4184150.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1044..>2017
                     /locus_tag="VVL53_002054"
     CDS             1044..>2017
                     /locus_tag="VVL53_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU4184151.1"
                     /translation="MRNKKQINLGIIFIICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLI"
BASE COUNT          705 a          288 c          360 g          664 t
ORIGIN      
        1 agacttgaaa tatgtttaag gagtttttat gaaaaacaaa ttttttctaa tagctatttt
       61 agctatgtgt atagttttta gcgcttgttc ttctaattct gttaaaaatg aagaaaatac
      121 ttctaaagag catgcgcctg ataaaatagt tttagatcat gctttcggtc aaactatatt
      181 agataaaaaa cctgaaagag ttgcaactat tgcttgggga aatcatgatg tagcattagc
      241 tttaggaata gttcctgttg gattttcaaa agcaaattac ggtgtaagtg ctgataaagg
      301 agttttacca tggacagaag aaaaaatcaa agaactaaat ggtaaagcta acctatttga
      361 cgatttggat ggacttaact ttgaagcaat atcaaattct aaaccagatg ttatcttagc
      421 aggttattct ggtataacta aagaagatta tgacactcta tcaaaaattg ctcctgtagc
      481 agcatacaaa tctaaacctt ggcaaacttt atggagagat atgattaaaa ttgattcaaa
      541 agccttaggt atggaaaaag aaggtgatga gttaatcaaa aatactgaag ctcgtatatc
      601 caaagaatta gaaaaacatc cagaaatcaa aggaaaaatc aaaggaaaaa aagtattatt
      661 tactatgatt aatgctgcag atacatcaaa attctggatt tatactagca aagatccaag
      721 agcaaattat ttaacagatt taggtctagt tttccctgaa tcattaaaag aatttgagag
      781 tgaagatagt tttgcaaagg aaatttctgc agaagaagca aataagataa atgatgctga
      841 tgtaatcata acttatggtg atgataaaac tcttgaagct ttacaaaaag atcctctttt
      901 aggtaaaata aatgcaatta aaaatggtgc cgttgctgta attccagata atacaccgtt
      961 agcagcctca tgcactccaa caccactttc aataaactat actattgaag aatacctaaa
     1021 tcttttagga aatgcatgca aaaatgcgaa ataaaaaaca aataaaccta ggcataattt
     1081 ttataatctg cctaggtctt cttattacaa tatttttgtc attaaagctt ggaacaaaag
     1141 aaattaatat cagagatttt ttagcagctt ttggaatggg taatacaaat gatgatttta
     1201 ttaaatcaat tatatataaa agaataccta gaactatttt tgcaatttta gcaggttcta
     1261 gtcttgccat aagcggtgta ttgatgcaat cagttactag aaacccaata gctgatccag
     1321 gtatactcgg tataaacaca ggagcaagtc ttagtgtagt aattggtctt tcttttttag
     1381 gaatttcatc aagcataagc catataagtt ttgcaatcat tggtggctta gtaagtgcaa
     1441 tttttgtata cgcgattgct gtaagcggaa aagcaggcct tacccctata aaacttgcct
     1501 tatcaggaac ttgtgttagt atggctttaa gcagttttgt aagtttttta attttaccga
     1561 ataataacgt cttagacaaa tttagatttt ggcaaatagg tagccttgga gcagctacat
     1621 tatcttctat atctacacta ctacctttta taattttagg tcacttgata gctatattta
     1681 tttcatcaga tttaaacgct ttagctatgg gtgatgaaat ggctgttggt cttggagtta
     1741 atgttaatag gataagatca cttgcaataa ttgcaagtgt gcttttatgt tcaagtatta
     1801 ctgcaattgg tggacctatt ggcttcgtag gtcttatagt tcctcacttt tgtggcttat
     1861 ttataagcaa agatatacgc acaatgacca tttcttcatc ttttataggt gcagagctct
     1921 tgcttatatg tgatataatc ggccgtatgt taggtaaacc aggtgaaatt gaagtaggga
     1981 taattactgc aataatcggg ggtccagtac ttattta
//
LOCUS       DAVHEW010000149         1980 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVHEW010000149 DAVHEW010000000
VERSION     DAVHEW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.149"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..>1980
                     /locus_tag="VVL53_002055"
     CDS             <1..>1980
                     /locus_tag="VVL53_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEU4184152.1"
                     /translation="ITNLDEANKIIVHYADGTKDYFNLSSSSEGLSNVKEYTITDLGI
                     KYTPNIVQKDNTTLVNDIKSILESVELQSQTMYQHLNRLGDYRVNAIKDLYLEESFTD
                     VKENLTNLITKLVQNEEHQLNDSPAARQMIRDKVEKNKAALLLGLTYLNRYYGVKFGD
                     VNIKELMLFKPDFYGEKVSVLDRLIEIGSKENNIKGSRTFDAFGQVLAKYTKSGNLDA
                     FLNYNRQLFTNIDNMNDWFIDATEDHVYIAERASEVEEIKNSKHRAFDNLKRSHLRNT
                     ILPLLNIDKAHLYLISNYNAIAFGSAERLGKKSLEDIKDIVNKAADGYRNYYDFWYRL
                     ASDNVKQRLLRDAVIPIWEGYNAPGGWVEKYGRYNTDKVYTPLREFFGPMDKYYNYNG
                     TGAYAAIYPNSDDIRTDVKYVHLEMVGEYGISVYTHETTHVNDRAIYLGGFGHREGTD
                     AEAYAQGMLQTPVTGSGFDEFGSLGINMVFKRKNDGNQWYITDPKTLKTREDINRYMK
                     GYNDTLTLLDEIEAESVISQQNKDLNSAWFKKIDREYRDNNKLNQWDKIRNLSQEEKN
                     ELNIQSVNDLVDQQLMTNRNPGNGIYKPEAISYNDQSPYVGVRMMTGIYGGNTSKGAP
                     GAVSFKHNAFRLWGYYGYENGFLGYASNKYKQQSKT"
BASE COUNT          737 a          288 c          350 g          605 t
ORIGIN      
        1 atcacaaacc tagatgaagc taataaaatt attgttcact atgcggacgg tacaaaagat
       61 tactttaact tgtcttctag cagtgaaggt ttaagtaatg taaaagaata tactataact
      121 gacttaggaa ttaaatatac acctaatatc gttcaaaaag ataacactac tcttgttaat
      181 gatataaaat ctattttaga atcagtagag cttcagtctc aaacgatgta tcagcatcta
      241 aatcgattag gtgactatag agttaatgca atcaaagatt tatatttaga agaaagcttc
      301 acagatgtta aagaaaactt aacaaaccta atcacaaaat tagttcaaaa cgaagaacat
      361 caactaaatg attctccagc tgctcgtcaa atgattcgtg ataaagtcga gaaaaacaaa
      421 gcagctttat tactaggttt aacttaccta aatcgttact atggagttaa atttggtgat
      481 gttaatatta aagaattaat gctattcaaa ccagatttct atggtgaaaa agttagcgta
      541 ttagacagat taattgaaat cggttctaaa gagaacaaca ttaaaggttc acgtacattc
      601 gacgcattcg gtcaagtatt ggctaaatat actaaatcag gtaatttaga tgcattttta
      661 aattataata gacaattgtt cacaaatata gacaatatga acgattggtt tattgatgct
      721 acagaagacc atgtctacat cgcagaacgc gcttctgagg tcgaagaaat taaaaattct
      781 aaacatcgtg cattcgataa tttaaaacga agtcacctta gaaatactat actcccacta
      841 ctgaatattg ataaagcaca tctttattta atttcaaatt ataatgcaat tgcctttggt
      901 agtgcagagc gattaggtaa aaaatcatta gaagatatta aagatatcgt taacaaagct
      961 gcagatggtt atagaaacta ttatgatttc tggtatcgtc tagcgtctga taacgttaaa
     1021 caacgactac taagagatgc tgttattcct atttgggaag gttataacgc tcctggtgga
     1081 tgggttgaaa aatatggccg ctataatacc gacaaagtat atactcctct tagagaattc
     1141 tttggtccta tggataagta ttataattat aatggaacag gagcttatgc tgctatatat
     1201 cctaactctg atgatattag aactgatgta aaatatgttc atttagaaat ggttggtgaa
     1261 tacggtattt cagtttacac acatgaaaca acacacgtca acgaccgtgc gatttactta
     1321 ggtggctttg gacaccgtga aggtactgat gctgaagcat atgctcaggg tatgctacaa
     1381 actcctgtta ctggtagtgg atttgatgag tttggttctt taggtattaa tatggtattt
     1441 aaacgcaaaa atgatgggaa tcagtggtat attacagatc caaaaactct aaaaacacga
     1501 gaagatatta atagatatat gaagggttat aatgacactt taactcttct tgatgaaatt
     1561 gaggctgaat ctgtgatttc tcaacaaaat aaagatttaa atagtgcatg gttcaaaaaa
     1621 atagatagag aataccgtga taacaataaa ttaaatcaat gggataaaat tcgaaatcta
     1681 agtcaagaag agaaaaatga attaaatatt caatctgtta atgatttagt tgatcaacaa
     1741 ttaatgacta atcgcaatcc aggtaatggt atctataaac ccgaagcaat tagctataac
     1801 gatcaatcac cttatgtagg tgttagaatg atgaccggta tctacggagg taatactagt
     1861 aaaggtgctc ctggagctgt ttcattcaaa cataatgctt ttagattatg gggttactac
     1921 ggatacgaaa atgggttctt aggttatgct tcaaataaat ataaacaaca atctaaaaca
//
LOCUS       DAVHEW010000150         1927 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVHEW010000150 DAVHEW010000000
VERSION     DAVHEW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.150"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            379..1854
                     /locus_tag="VVL53_002056"
     CDS             379..1854
                     /locus_tag="VVL53_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4184153.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          561 a          364 c          444 g          558 t
ORIGIN      
        1 gatgatgttt tctagcaaat ataaacccga gtaaaaaatg cctacggaca ggcagggttg
       61 aatgccgaag cgtggttgaa aagccacatt attgataggg ttaaaagcct acttttataa
      121 gttgatgtta ggacacttgt cctaattcat aaatttttag tgtggtgaaa gcacacgtca
      181 tcttgtgaaa cgatcaataa agtacgtaat atttgctact agagagttag gaaacatcgg
      241 gaacagacat actcaacaga aaccaaaata aacacgtcag aagattgcag agcaggtgaa
      301 aacctgctct tttttcatga gtcaaccttt agttccttag ttttcataag gtcctaaaaa
      361 tattgaaagg agtatgtttt gaaagagtta gatcaaaacc aagccccaat ttatgaggcc
      421 ttggtgaagt tgcgcaagaa aaggattgtt ccctttgatg ttccaggtca caagcgtgga
      481 cggggaaatc cagaacttgt cgaactctta ggagaaaaat gtgtaggcat tgatgtcaat
      541 tcgatgaaac ccttggataa tttaggccat cctatttcga ttattcgtga tgcagaggag
      601 ctggctgcag atgcttttgg agctagccat gcctttctaa tgattggtgg aacaacttca
      661 tcggtgcaga ctatgattct ggcaacctgc aaggcaggag ataagattat tctgccacga
      721 aatgtccata aatctgctat caatgcgttg gttctatgtg gtgccattcc catctatatc
      781 gagatgagtg tagatcctaa gattggtatc gctttaggtc ttgaaaatga ccgagtagca
      841 caggccataa aggaccatcc agatgctaag gctatcctaa tcaacaatcc tacttactac
      901 ggcatctgtt cagacctaaa ggggttgaca gaaatggctc atgaagctgg catgatggtt
      961 ttagtagatg aagcccacgg agcgcatttg catttcactg ataaacttcc aatttctgct
     1021 atggatgcag gggctgatat ggcagcagtt tccatgcata agtctggtgg gagtttgacc
     1081 caaagctcca ttttacttat cggggagcag atgaattctg aatacgttcg tcagataatt
     1141 aacctgaccc agtctacatc tgcctcttac ttgttgatgg ctagtttgga tatttcacgt
     1201 cgcaacttgg cccttcgtgg taaagagtcg tttgaggaag tcattgagct atctgagtat
     1261 gcccgccgtg aaatcaatgc tatcggtggc tactatgcct actcaaaaga gttaatagac
     1321 ggtgtttcgg tttgcgattt tgacgtaact aagttgtcag tttacactca gggtattggc
     1381 ttaacaggta tcgaggttta tgacctcttg cgagacgaat acgacattca gatcgagttt
     1441 ggtgatatcg gcaatatctt ggcctatatt tccatcggcg accgcatcca agacatcgag
     1501 cgcttggttg gtgctctggc tgatattaag agactctatt caagagatgg aaaagatttg
     1561 atagcaggag aatatattca gcccgagtta gtgctgtctc cgcaagaagc cttctattca
     1621 gaaagaaaaa gtttaacttt ggatgattct gttggacagg tctgtggaga atttgttatg
     1681 tgttaccctc caggtattcc tatcttggct cctggtgaac gcattacacg agaaattgtc
     1741 gactatatcc aattcgccaa ggaacgtggt tgctccctcc aagggacgga agatccagag
     1801 gtcaatcata tcaacgttat taagagaaag acaaactata agaaaagtca atagttttat
     1861 ctaaactatt tcttatttca atttgatgat ttggcgatga ttttagagca cggcaaaaag
     1921 cccttga
//
LOCUS       DAVHEW010000151         1927 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVHEW010000151 DAVHEW010000000
VERSION     DAVHEW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.151"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..134)
                     /locus_tag="VVL53_002057"
     CDS             complement(<1..134)
                     /locus_tag="VVL53_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4184154.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     T"
     gene            216..667
                     /locus_tag="VVL53_002058"
                     /pseudo
     CDS             216..667
                     /locus_tag="VVL53_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            832..1131
                     /locus_tag="VVL53_002059"
     CDS             832..1131
                     /locus_tag="VVL53_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU4184155.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1240..>1927)
                     /locus_tag="VVL53_002060"
     CDS             complement(1240..>1927)
                     /locus_tag="VVL53_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4184156.1"
                     /translation="NYAYRRIYLELRNRGYLVNHKRVQGLIKVLNLQAKMRQKRKYSS
                     HKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLLPVLDGFNSEIIAY
                     NLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRK
                     GNSPDNGMMEFFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLS
                     LVQYRTKSFG"
BASE COUNT          620 a          369 c          333 g          605 t
ORIGIN      
        1 gttttctctt ttagctttaa ccagccataa atggtattac gtgagatttg gaaaacgtgt
       61 gatgcttctg ttatactacc tgttcgctca caataagaga gaactttttt acgaaaatct
      121 attgaatatg ccataagaag attataccac attgtgtact atttttggtt catttcacta
      181 taataataac gatactagat aatgaggaaa atgcaatggc aaacccaact ttcggagaaa
      241 aaaagtgaat acagactata ttgctcgcta tggcgtatat gcagttatcc ctaatcctga
      301 acaaaaacaa attgttcttg ttcaagaacc aaatggtgct tggttcctac catgtggaga
      361 aattgaagca ggtgaaaatc atcaggaagc cctaaagcat gagttgattg aagagcttgg
      421 tttcacagca gaaattggta cctattacgg acaagctgac gaatatttct attctcgtca
      481 tcgtgatacc tactactaca atcctgccta cctctatgaa gcaactcctt tcaaagaagt
      541 acaaaagcca ctagaaaact ttaatcatat tgcctggttc cctattgacg aggctatcaa
      601 aaaccttaaa cgtggtagcc ataaatgggc cattgaatct tggaaaaaac agcataagat
      661 tggctaatac tcttcgaaaa taaaattcaa accacgtcag cgtcgcctta ccgtactcaa
      721 gtatagcctg cggctagctt cctagtttgc tctttgattt tcattgagta taaatcaagc
      781 gactttattc aaaaagtgct ataatagaat tagaaaatcg gaggaaatat tatgaagctt
      841 atcaatacga caaactcaca ctcgcaactt gtaaaaagtc agctagaaag taccgatgca
      901 acccttgttg aggtttattc tgcagggaat acagatgtta tttttaccca agctccgctt
      961 cactatgaaa tcctcatctc aaacaaacac cgtgctattc gtgagcctga aatcgaaacc
     1021 attcaagaat ttttcttgaa acgtaaaatt gataaggcct ctgttgatga agccaatatc
     1081 aagacactct actcagagaa attaattgga atttcaatcc caatcaagta agaaaattga
     1141 gtaaaaactc gatttgagag aaagtccaat aagtctttca ataataaaac gcatagtagc
     1201 aagagttttg tactgacccc aaaaagttag acaattaatt tatccgaagg atttagttct
     1261 gtattgcaca aggctaagtc cttttagttt taccttaatt cgtttattgt tgtagtaatc
     1321 aatatagtct acaatggctt gttccaattg cttaagcgac tgaaacgact tctcataacc
     1381 gtaaaacatt tccgatttca gaatcccaaa gaagaactcc atcataccat tgtctgggct
     1441 gttaccctta cgtgacatgg atgcttgaat tcccttaccc tcaagaaact gatgataaga
     1501 atcgtgttga tattgccagc cttggtcact atggagaatc gtattctcgt agtgcttctc
     1561 tgtgaatgcc tgttccaaca ttgtttgtac ttgttctaag ttgggtgaag ttgaaagatt
     1621 ataggcgata atttcgctat taaagccatc taaaactggt aataagtaaa gcttttgagt
     1681 acttgctgga atggcaaatt ctgtcacatc tgtgtagcac ttttccattg ttttagagcc
     1741 ttcaaattgg ccttgaatga gattctctgc tttcttacca acgtctcctt tatgagaaga
     1801 atattttcgt ttttgtcgca ttttagcttg taaattgagt acttttatca agccttgaac
     1861 tcttttatga tttaccagat aaccacgatt tcttagttct aaataaatcc gacgataagc
     1921 ataattt
//
LOCUS       DAVHEW010000152         1894 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVHEW010000152 DAVHEW010000000
VERSION     DAVHEW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.152"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..534
                     /locus_tag="VVL53_002061"
     CDS             <1..534
                     /locus_tag="VVL53_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4184157.1"
                     /translation="LNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLENGVVISDKIP
                     ESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTP
                     EFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKIQ
                     PESSMFQCIKEKLEALF"
     gene            914..1717
                     /locus_tag="VVL53_002062"
     CDS             914..1717
                     /locus_tag="VVL53_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU4184158.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPAILLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADMVKKIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDLS"
BASE COUNT          632 a          297 c          349 g          616 t
ORIGIN      
        1 ttaaactcat cgtatttaga ttccgataac cattattttt ggcttgatgg agatcaaaac
       61 actaatgtta gtgaatcaaa taatttaatg aactatcttg aaaatggtgt tgttatatca
      121 gataaaattc ctgaatcaga taataaaaat cttgatgata ttataaaatt gataacggga
      181 tgtccaatta aatttaatgt ttcaggtaat aaagggcaaa aaaataatat tgaattaatt
      241 gcgaaacaaa gaagctttat agattattgg gctaaatacg tttcttatct tcctttccca
      301 accccagagt tttttttagc taatttgtgt aacagcgtag atagggaagg ttatgatttt
      361 tctaaagatg gcaacggaaa agaatatttt agaaaaaaaa ctcaagtagc gttaggaata
      421 gagaatataa cttctgaaga tattttccag gagcaaagaa gagctgtttc aaaaatacag
      481 ccagaatcat caatgtttca gtgtattaaa gaaaaattag aggcattgtt ctaattcctt
      541 agattaggta ctacataaca aattttttaa attcaaaccc gcaccaaaaa caacattgca
      601 actttcttct caggtaatgt gttgggattt gaacagacca acgagttcat agtggtgtat
      661 gccaatgact ttgataagtc tacgcgtcag acttattagt tcatttatcc agatatatct
      721 cgataatcat attatttagt caaagaacat cccatatgag ggcatgaaat gctaagttta
      781 gcttgctcac tattaagatg aagtatatat atttcataga acctattgtg aaggattttt
      841 ataattttcg ccaattccat acctatttca caatttaccg atgacacaga aaaacaattg
      901 aaaggatatt tctatgcaac caacttacaa cattgacaat ccaaacttgt cttatgaagc
      961 gaagcgtgat ttatggcgga taggttttgg tctgcagaaa gttgacaatc tagtgccatc
     1021 agcgtatatg gaatctttgg ctgagaaaca gtcccggggt gaactgactt atgagcaggt
     1081 ttatgaggat gcaacggctt atcatcatac cattgatgcg agtacagagg aggcagactt
     1141 ggtttctcta cgtattgtag aactattgtc tcgaagaggc tttagcttca gtcctgcgat
     1201 cttacttgct attcataagg agttgtttca agatatattt gaaccctcga ttccggtagg
     1261 tcaatttcgt cagactaata tcacaaagaa tgaacctgtt ttgaatggtg aaagtgttgt
     1321 gtactctgat tactccatga ttcaaatgac cttggattat gattttaatc aggaaaaaca
     1381 agttgcatat gcgacactaa cccaggcgga tatggttaaa aaaatccagc attttatttc
     1441 aggaatctgg cagattcatc catttcgcga aggaaacact cggacggtaa cggtattttt
     1501 gattcagtat cttcgtgagt ttggttttga tattgataat acaccatttc agcaacattc
     1561 caagtatttt cgtgatgcct tagtgttaga taatgcaaag attttacagc gacgtcctga
     1621 gtttttaaca gctttttttg aaaatctctt gctcggtggt caaaatgatt tgtcttcaga
     1681 aaaaatgtat ctagatttag acctcgatct ttcataatcc taatactgag taaacattga
     1741 attttaggaa aaaatgaagt aaatattctc acaagaaaac gtatatcatc aaagtttggc
     1801 tctttgtcaa ttgtagtggg ttgaagaaaa gctaagttcg agaaaggaca aatttcgtcc
     1861 tttctttttt gatgttcaga gcgataaaaa tccg
//
LOCUS       DAVHEW010000153         1894 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVHEW010000153 DAVHEW010000000
VERSION     DAVHEW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.153"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(1..220)
                     /locus_tag="VVL53_002063"
                     /pseudo
     CDS             complement(1..220)
                     /locus_tag="VVL53_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(296..1072)
                     /locus_tag="VVL53_002064"
     CDS             complement(296..1072)
                     /locus_tag="VVL53_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4184159.1"
                     /translation="MFAHLDNSVFLKRNKIWKKTLTYPVEREEITYKRKKAKGKRQAL
                     LAQFDSEEVHHQVEESICPDCQGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQA
                     CSDKNPSDKIVKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWAKMGLPI
                     TRKEIANWHIKASQYYLEPLYNLLREKLLEQALLHADETSYRVLESDSQLPYYWTFLS
                     GKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(996..1190)
                     /locus_tag="VVL53_002065"
     CDS             complement(996..1190)
                     /locus_tag="VVL53_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4184160.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
     gene            complement(1267..1607)
                     /gene="tnpB"
                     /locus_tag="VVL53_002066"
                     /pseudo
     CDS             complement(1267..1607)
                     /gene="tnpB"
                     /locus_tag="VVL53_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1588..1773)
                     /locus_tag="VVL53_002067"
     CDS             complement(1588..1773)
                     /locus_tag="VVL53_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184161.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
BASE COUNT          547 a          401 c          353 g          593 t
ORIGIN      
        1 ttcttgacgt ttctgtagtc gttcatcagc tggcaaagcc tcccagtctc tttccaagga
       61 aaataactga tcacaataag ctaaaccttt agctcctaag gatgatttat ccgcttgctt
      121 gggggggcgc ttcaaaaaac ttccttctca catgcgccca acatccaaca agttcagctt
      181 cttccagttg acgataagct tctagctgtt cgcagtttac caagcttagc gtcgccttac
      241 tcctaaacct tggactgcta tcgcataggc agaactagag gactaaagtc ctaagttact
      301 gccgcaacat gtcacaatga acatagccag aataatctcc taggaattct tgtactacta
      361 aaccactccg acgctgatca tggtggtaca gcgtgattgc ttgattctca gctttcccag
      421 acaaaaaagt ccaatagtaa ggcaactgac tatcactctc tagaacccga taagaggttt
      481 catccgcatg aagaagagct tgttctaaca acttttctcg taaaagatta taaaggggct
      541 ccaaatagta ttgactcgcc ttgatatgcc aattagcaat ttccttacgt gtgattggta
      601 aacccatctt agcccaatct tcttcttggc gataattggg taccttcaga ttaaacttct
      661 gatggatggt gtgagcgata atagaagctg agccaaggct atgcgccaaa ggggctttag
      721 gaataggagc tttcacgatt ttatcactcg gatttttatc actgcatgct tggcacttat
      781 aagcgtgttg gatatgatct attcgtttta attgcgcagg aataaagact aattcttgtc
      841 gttgaagggt tgctccaatc tcttttagat ctccctgaca atcagggcaa atgctctctt
      901 ctacttgatg atgaacttct tctgaatcaa attgggcaag aagagcttga cgtttccctt
      961 tagctttctt acgtttatag gtgatttctt ctctttcaac tgggtaagtc agagtcttct
     1021 tccatatttt gttcctcttc aaaaagactg agttgtccag atgggcaaac acttttctca
     1081 gaggattttc catagagctt ttgcgttaga taagccactt gttcacgaag aagggcaagt
     1141 tcattggtga gactatcaat tgtagcactc tgttgttgaa tgattttcaa tcttcttcca
     1201 tgggcttcct ctccttattt cctaacttta ttataactaa aaagaaagtc ctcatttcaa
     1261 tagaaatcac gactttctga taaatttatt tttggagtga tagaaaagcc cttcataagc
     1321 caatctactt gttcaggtgt gagagctttg acatcctttt ctgtactggg ccaagtcagt
     1381 tttccgttct caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaagggct
     1441 ttaaagcggt ctttacgtcc accacaaaag agaaaaactt gaccggagaa aggatctaat
     1501 tcaaagtggg ttttaaccag ataagccaat gaatcaatgc cttgcctcat atccgttttc
     1561 ccacatacga gatagacctg ccctaggcta gatagatgaa ttgtcataga gcaacacttt
     1621 atccaaaagc tgttctacca tttcgagatt gagagattga aaaaaactta cttcaagctt
     1681 gccaatacga attttaagca gaatatcatt tctcttttta gaatcaaaac gacgagattg
     1741 tggaatctct acaggaacaa taggttgtgg cattaaaata tcctccaata gttttacttt
     1801 actaatagta tacaggagga actccatgaa tgatagatac cttgttatga cgcgattact
     1861 atattagaaa gttggctctc taattaaatt aatt
//
LOCUS       DAVHEW010000154         1845 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVHEW010000154 DAVHEW010000000
VERSION     DAVHEW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.154"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            38..>1845
                     /gene="pspA"
                     /locus_tag="VVL53_002068"
     CDS             38..>1845
                     /gene="pspA"
                     /locus_tag="VVL53_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU4184162.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEESPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYEDDQKRTEEKARKEAEASQKLNDVALV
                     VQNAYKEYREVQNQRSKYKSDAEYQKKLTEVDSKIEKARKEQQDLQNKFNEVRAVVVP
                     EPNALAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAA
                     LQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAP
                     KPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQE
                     NGMWYFYNTDGSMAIGWLQNNGSWYYLNANGAMATGWVKDGDTWYYLEASGAMKASQW
                     FKVSDKWYYVNSNGAMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMAT
                     GWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGS"
BASE COUNT          712 a          342 c          407 g          384 t
ORIGIN      
        1 agtctaaaaa tgactatcag aaaagaggta aatttagatg aataagaaaa aaatgatttt
       61 aacaagtcta gccagcgtcg ctatcttagg ggctggtttt gttacgtctc agcctacttt
      121 tgtaagagca gaagaatctc cacaagttgt cgaaaaatct tcattagaga agaaatatga
      181 ggaagcaaaa gcaaaagctg atactgccaa gaaagattac gaaacggcta aaaagaaagc
      241 agaagacgct cagaaaaagt atgaagatga tcagaagaga actgaggaga aagctcgaaa
      301 agaagcagaa gcatctcaaa aattgaatga tgtggcgctt gttgttcaaa atgcatataa
      361 agagtaccga gaagttcaaa atcaacgtag taaatataaa tctgacgctg aatatcagaa
      421 aaaattaaca gaggtcgact ctaaaataga gaaggctagg aaagagcaac aggacttgca
      481 aaataaattt aatgaagtaa gagcagttgt agttcctgaa ccaaatgcgt tggctgagac
      541 taagaaaaaa gcagaagaag ctaaagcaga agaaaaagta gctaagagaa aatatgatta
      601 tgcaactcta aaggtagcac tagcgaagaa agaagtagag gctaaggaac ttgaaattga
      661 aaaacttcaa tatgaaattt ctactttgga acaagaagtt gctactgctc aacatcaagt
      721 agataatttg aaaaaacttc ttgctggtgc ggatcctgat gatggcacag aagttataga
      781 agctaaatta aaaaaaggag aagctgagct aaacgctaaa caagctgagt tagcaaaaaa
      841 acaaacagaa cttgaaaaac ttcttgacag ccttgatcct gaaggtaaga ctcaggatga
      901 attagataaa gaagcagaag aagctgagtt ggataaaaaa gctgatgaac ttcaaaataa
      961 agttgctgat ttagaaaaag aaattagtaa ccttgaaata ttacttggag gggctgattc
     1021 tgaagatgat actgctgctc ttcaaaataa attagctact aaaaaagctg aattggaaaa
     1081 aactcaaaaa gaattagatg cagctcttaa tgagttaggc cctgatggag atgaagaaga
     1141 aactccagcg ccggctcctc aaccagagca accagctcct gcaccaaaac cagagcaacc
     1201 agctccagct ccaaaaccag agcaaccagc tcctgcacca aaaccagagc aaccagctcc
     1261 agctccaaaa ccagagcaac cagctccagc tccaaaacca gagcaaccag ctaagccgga
     1321 gaaaccagct gaagagccta ctcaaccaga aaaaccagcc actccaaaaa caggctggaa
     1381 acaagaaaac ggtatgtggt atttctacaa tactgatggt tcaatggcaa taggttggct
     1441 ccaaaacaac ggttcatggt actacctaaa cgctaacggc gctatggcaa caggttgggt
     1501 gaaagatgga gatacctggt actatcttga agcatcaggt gctatgaaag caagccaatg
     1561 gttcaaagta tcagataaat ggtactatgt caacagcaat ggcgctatgg cgacaggctg
     1621 gctccaatac aatggctcat ggtactacct caacgctaat ggtgatatgg cgacaggctg
     1681 gctccaatac aacggttcat ggtattacct caacgctaat ggtgatatgg cgacaggatg
     1741 ggctaaagtc aacggttcat ggtactacct aaacgctaac ggtgctatgg ctacaggttg
     1801 ggctaaagtc aacggttcat ggtactacct aaacgctaac ggttc
//
LOCUS       DAVHEW010000155         1756 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVHEW010000155 DAVHEW010000000
VERSION     DAVHEW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.155"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..1520)
                     /locus_tag="VVL53_002069"
     CDS             complement(<1..1520)
                     /locus_tag="VVL53_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU4184163.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGKGVDSVYNVPIYYRLTVTNDGLKLTFTYTVTYVDPKTKTLGNISKKMSN
                     GYSIYNTGTSIQTMLTLGSGLGTPSDVKNSITNKNGGQVQYYNTSTMTKRGSRYTWGN
                     GAQMNGWQAKREYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFNGRGKVVESST
                     TSQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASASTSASASASISAS
                     ESASTSASASASTSASASASTSASASASTSASASASTSASESASTSASESASTSASA"
     gene            complement(1518..1631)
                     /locus_tag="VVL53_002070"
                     /pseudo
     CDS             complement(1518..1631)
                     /locus_tag="VVL53_002070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          465 a          369 c          376 g          546 t
ORIGIN      
        1 gctgaggcac tggttgaggc cgattcagat gcactggttg aggccgattc agacgctgag
       61 gtacttgctg aagctgacgc actggtgctt gctgaagctg aagcactcgt tgatgcggaa
      121 gctgaggcgc tggtacttgc tgaggctgaa gcactcgttg atgccgattc agacgctgag
      181 atacttgctg aggctgacgc actggttgaa gccgacgcac tggtgcttgc tgaagctgag
      241 gcgcttgtgc ttgctgaggc tgaggcactg gttgatgctg aggctgaggc gctttgacta
      301 gcacttactg agagtgactt agactgtgaa agtgactgac tggtcgtgct agattcaact
      361 acctttcctc taccattgaa gtagttagtt ctaatgttat ctgttttagc agcgtaaggg
      421 gtaaatgtaa aggatgtatc cgttccagta attggtacag tccaagatga tgttaatcca
      481 tattctctct tagcctgcca accattcatt tgggcaccat ttccccaagt atacctacta
      541 ccccgcttcg tcattgtaga tgtattatag tattgaacct gtccaccatt tttgttagta
      601 atggagttct ttacatctga aggggtacca agaccactgc caagggttaa cattgtttgt
      661 attgaagttc ctgtattata gatagaatat ccattactca tcttttttga tatattacca
      721 agagtctttg ttttaggatc cacatacgta accgtatagg taaaggtcag ttttaagcca
      781 tcatttgtca ctgtcaatcg atagtaaatt ggaacattat ataccgaatc tacaccttta
      841 cctgtataaa cttttgtctc acttttagca atgtttagac ttgcattaat tgcaggggcg
      901 ccatttacaa tcgtattgcc agaaaaaaca gcagctttta tagaagccag caattgctcg
      961 atggaatcca ctgaacgctt gcgtcgctta gcataagatt ggagattgac atttgtaact
     1021 gatgctacat aatcactagc tggtttctta cgatcttctt cgaccttctt agcagttgct
     1081 tctgtagcgg cagctgtttg tgatcctacc acagtagatg atgcagagat acttgtcgaa
     1141 gccgatgtac ttgctgattc tgatgcactt gtactcgctg aggtcgaagc gctggttgag
     1201 gctgactcag atgcagatgt acttgccgac tctgaagctg acaaactagt tgaactcgct
     1261 gaattacttg tagatatcgt acctagaact actgtaccat ttgttgccaa agcatccgtt
     1321 ttctctacag ttttttcaag tactgccgac tcatttgtaa ataccttcgt ttgagttgca
     1381 acggttccac ttatgacagt tcccgcagca actatcccct tgaggatatc aagcccagta
     1441 attgaatgac ttactttatc ttctaccgtt tcggtcatga cctgagtagt atcaacacca
     1501 ccacgcatga ccttaaacaa gccaaaaaga gaagtcgagg cccgtagcca atgcttccct
     1561 gatttgatta atttgaaccg agttactcgg tctgtttctc tatattcacc cttctgacgt
     1621 ctaaaaaata ttttgaaatt tcctttcgag tattattttc atgtatggat ataaataaat
     1681 taccatacta taattataga atagggaacc caaaatagca agaaacttga ggtatttttg
     1741 ctaaaaaaaa aaagca
//
LOCUS       DAVHEW010000156         1604 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVHEW010000156 DAVHEW010000000
VERSION     DAVHEW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.156"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..198)
                     /locus_tag="VVL53_002071"
     CDS             complement(<1..198)
                     /locus_tag="VVL53_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4184164.1"
                     /translation="MKLSYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEM"
     gene            440..780
                     /gene="tnpB"
                     /locus_tag="VVL53_002072"
                     /pseudo
     CDS             440..780
                     /gene="tnpB"
                     /locus_tag="VVL53_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(813..1604)
                     /locus_tag="VVL53_002073"
                     /pseudo
     CDS             complement(813..1604)
                     /locus_tag="VVL53_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          486 a          322 c          275 g          521 t
ORIGIN      
        1 catttcttgt tttaaatcag gagaatagta acgatttttt ccttttttga cgaactctat
       61 tccgtaacga tcaatcaatt taatcatata cctaagatta gaattgttta tcccaaattt
      121 atttgaaagc ttctctaagc tatatccttg ttttctaagt tcatagatct gaactttatc
      181 atcataactt aatttcataa taaaaacacc ccaaaaagtt agattttttc tgtctaactt
      241 tttggggggc agtacactac acctactatt atacgttttt atcattttga aatctactgc
      301 taatcaatga agaagcaaag atcaaactag gcagctagtt acaagttacg taagcgcgtc
      361 ataacaaggt atctatccta attccccaac ttccagtata ctagataaaa aataattatc
      421 ggaggaaagg tattgtacta tgataattca actaagtgat ttaggtcaag ttcaccttgt
      481 ttgtagcaag atagatatga ggcagggaat agactcatta gcctatgtag ttaaaaccca
      541 ctttgaattg gatcctttct ccggtcaagt ctttctcttt tgtggtggac gtaaagaccg
      601 ctttaaagcc ctttactggg atggtcaagg attttggcta ctatataaac gctttgagaa
      661 cggcagactg acttggccca gtacagaaaa ggatgtcaaa gctctcacac ctgaacaagt
      721 agactggctt atgaagggct tttctatcac tccaaaaata aatccatcag aaagtcgtga
      781 tttctattga aatgaggact ttctttttat actcatctgc tttcaaaaag cattctagtc
      841 catctccgat taacgatggg ctttatcacc tccttctcca gtccttgtat gatatcttaa
      901 agttgattca tgacatcttc caaagtttga aaggctttat ttttaaatcc acgtttacga
      961 atctctttcc acacttgttc aatggggttc atctctggtg tgtacggagg aataaatgca
     1021 aagccaatat tagtcggaat ctttaaggta cttgatttat gccatatagc attgtccata
     1081 acgaataaaa gataatcatc tggataagct tgtgaaagct cttctaaaaa gacgttcatc
     1141 cactcagtat tacatccacc agctattaag aaaaatgatt cgcctgtatg ggtccataac
     1201 aatagcgaaa ttctcgtata tagtgactat ggacatgtgg acctactcct attggagacc
     1261 aacaagatcc cagtttactg attctaccga agccagcctc atcttggtac atcaagcgaa
     1321 ccttatgaaa acgtctactg gttttaaaac gctttctttt cttcttgaat tgagatttta
     1381 tttttagacg cgacaatggt ttgagcgtct gctttcttag gatgttctag acgtggcata
     1441 atatttcgcc aaccatggcg cttcaacagt tgatagaagg catcacgtgt gtaggaacga
     1501 cctgactcct ttttataagc ctgaaataag gcatcaattg taacaaattc tcctgcctct
     1561 gcagccttca aatgacgggc aagaaaggct ttctcttccc caac
//
LOCUS       DAVHEW010000157         1535 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVHEW010000157 DAVHEW010000000
VERSION     DAVHEW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.157"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(190..951)
                     /locus_tag="VVL53_002074"
     CDS             complement(190..951)
                     /locus_tag="VVL53_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HEU4184165.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(951..1427)
                     /locus_tag="VVL53_002075"
     CDS             complement(951..1427)
                     /locus_tag="VVL53_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4184166.1"
                     /translation="MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
BASE COUNT          458 a          305 c          207 g          565 t
ORIGIN      
        1 aatttttcta tatttcgttg cagtccaatt agaaaatagc ttgtaatcat aagaaacagg
       61 tgtatgcaaa tgaattaaag tcttgtgaaa gctaccgaat ttctctttgg aatttgttag
      121 ttcttgataa acagacttta tcttgttatt aaaccctctc atttctattc tcctaaaaat
      181 atcaattgtt tatttttcaa gtaattcatt aagtctcttt tcccccacct gcaacatctc
      241 cttctctacc tgactccact ccccaaagag taactcttga agaacatctg ctgctgaagt
      301 tgtattttct tttgaatcat atacacaact tctatctcgt tggtaaattt gaattctttc
      361 aaagatagct agttcttcca attttcgtgt gttatcaact agatgattta caatgaaatc
      421 atgatgttct tttggagttg cgcgtgcttg atttggattg ataatgtaca gttcttcata
      481 acggataaga gtacttagat acgacaattc aggctttgtc gcaattaagg ccaattgtac
      541 ttcatatccc ttatttttca agagttgtgc tgttttcttt ggaacatcaa ctgttcgtaa
      601 agttccctct atcaaaagat tgtatctcaa actactcaat tttgttacta aagactctac
      661 catttttcct gcaaaatctt tggtatattc tacactgtct ttgccatatt cttgctgcag
      721 ttctaaatag tgtggatgct gagaacgaaa actatcacca tctatgataa caatatttcc
      781 ttgaaattct ttctgtttaa tacgatgaat tgtagtctta ccggcaccac tttgccctcc
      841 aagcaaaatc gctataggtt gcttactgga cttttttcct cttgtcagtg aacgaagatt
      901 ccttgctaaa gcatgtttga attcactatc agtataatct tggatttcca ttaggccacc
      961 atccgttttt cagatatctc taacatacgt tcaattccat ccaaatagcc actatatctc
     1021 tctatttcat caaaagtttc tactagatag atattcgtat gaatcaaatc agatagatca
     1081 tcactcatta aaatccaagg attagactca tcatcaatgc taattccctg actatcttga
     1141 taacgataga gtcgagataa tagattagca cctctttctt tcacaatttc aatttttaaa
     1201 gtcaattcat aatcttcaac aggattgagc attttatctt ctcctacaat atcgacataa
     1261 gatacattaa acttctgaca aatgatgtct attaattcgg tagagactga actcgttcca
     1321 ttttcataac gactcagact atttcgtgaa atacctacaa tccgtgcaaa ttcgagttgt
     1381 gttaagtcat gtgttttacg caaggatttt atgttctttc caatcatagc aaactccttt
     1441 atttttataa atccattata gcataaaaaa gcataacgca ccaaaaatgg tgcgttattt
     1501 cttgtttttc tatcctaaac tttacttcta taaaa
//
LOCUS       DAVHEW010000158         1517 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVHEW010000158 DAVHEW010000000
VERSION     DAVHEW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.158"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(88..175)
                     /locus_tag="VVL53_002076"
     tRNA            complement(88..175)
                     /locus_tag="VVL53_002076"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(139..141),aa:Ser,seq:gct)
     gene            complement(182..255)
                     /locus_tag="VVL53_002077"
     tRNA            complement(182..255)
                     /locus_tag="VVL53_002077"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Ile,seq:gat)
     gene            complement(291..361)
                     /locus_tag="VVL53_002078"
     tRNA            complement(291..361)
                     /locus_tag="VVL53_002078"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Gly,seq:tcc)
     gene            complement(381..453)
                     /locus_tag="VVL53_002079"
     tRNA            complement(381..453)
                     /locus_tag="VVL53_002079"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Phe,seq:gaa)
     gene            complement(457..530)
                     /locus_tag="VVL53_002080"
     tRNA            complement(457..530)
                     /locus_tag="VVL53_002080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(494..496),aa:Met,seq:cat)
     gene            complement(544..633)
                     /locus_tag="VVL53_002081"
     tRNA            complement(544..633)
                     /locus_tag="VVL53_002081"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Ser,seq:tga)
     gene            complement(644..717)
                     /locus_tag="VVL53_002082"
     tRNA            complement(644..717)
                     /locus_tag="VVL53_002082"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(681..683),aa:Ile,seq:cat)
     gene            complement(731..804)
                     /locus_tag="VVL53_002083"
     tRNA            complement(731..804)
                     /locus_tag="VVL53_002083"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(768..770),aa:Met,seq:cat)
     gene            complement(810..883)
                     /locus_tag="VVL53_002084"
     tRNA            complement(810..883)
                     /locus_tag="VVL53_002084"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Pro,seq:tgg)
     gene            complement(899..972)
                     /locus_tag="VVL53_002085"
     tRNA            complement(899..972)
                     /locus_tag="VVL53_002085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(936..938),aa:Arg,seq:acg)
     gene            complement(982..1067)
                     /locus_tag="VVL53_002086"
     tRNA            complement(982..1067)
                     /locus_tag="VVL53_002086"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Leu,seq:taa)
     gene            complement(1075..1146)
                     /locus_tag="VVL53_002087"
     tRNA            complement(1075..1146)
                     /locus_tag="VVL53_002087"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1112..1114),aa:Gly,seq:gcc)
     gene            complement(1169..1241)
                     /locus_tag="VVL53_002088"
     tRNA            complement(1169..1241)
                     /locus_tag="VVL53_002088"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1206..1208),aa:Thr,seq:tgt)
     gene            complement(1260..1341)
                     /locus_tag="VVL53_002089"
     tRNA            complement(1260..1341)
                     /locus_tag="VVL53_002089"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1305..1307),aa:Leu,seq:tag)
     gene            complement(1347..1419)
                     /locus_tag="VVL53_002090"
     tRNA            complement(1347..1419)
                     /locus_tag="VVL53_002090"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1384..1386),aa:Lys,seq:ttt)
     gene            complement(1444..1516)
                     /locus_tag="VVL53_002091"
     tRNA            complement(1444..1516)
                     /locus_tag="VVL53_002091"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1481..1483),aa:Asp,seq:gtc)
BASE COUNT          421 a          421 c          332 g          343 t
ORIGIN      
        1 tcagattagt aattcaatta agttttacgg atctgaagct ttattgtctc taaaaataaa
       61 aaagagagtt acaaactctc attaaaacgg agaataaggg attcgaaccc ttgcgccagt
      121 tacccgacct aacgatttag caaaccgtcc tcttcagcct cttgagtaat tctccaatta
      181 atgggcacga gtggactcga accaccgacc tcacgcttat caggcgtgcg ctctaaccac
      241 ctgagctacg cgcccaagtt aaaaaacttg gtaatttgaa caaagttcaa agcgggtgac
      301 gagaatcgaa ctcgcgacaa cagcttggaa ggctgtagtt ttaccactaa actacacccg
      361 cataaatact atcaataaaa tggcgcgaga cggaatcgaa ccgccgacac atggagcttc
      421 aatccattgc tctaccaact gagctaccga gccttattgc gggagcagga tttgaaccta
      481 cgaccttcgg gttatgagcc cgacgagcta ccgagctgct ccatcccgcg ttaataatat
      541 aaaaggagga tgtgggattc gaacccacgc acgcttttac acgcctgacg gttttcaaga
      601 ccgttccctt cagccggact tgggtaatcc tccaatattc aaatggacct tgtaggactt
      661 gaacctacga ccactcggtt atgagccgag agctctaacc agctgagcta aaggtccgac
      721 aagatcatta tagcggcgaa ggggatcgaa cccccgacct cccgggtatg aaccggacgc
      781 tctagccagc tgagctacac cgccatgaat cgggaagaca ggattcgaac ctgcgacacc
      841 ttggtcccaa accaagtact ctaccaagct gagctacttc ccgagttaaa tagaaaaatg
      901 caccctagag gagtcgaacc tctaaccgcc tgattcgtag tcaggtactc tatccagttg
      961 agctaagggt gctccatatt atgccgagga ccggaatcga accggtacga tcgttaccaa
     1021 tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca ccccggcctc tctaagcgaa
     1081 cgacgggatt cgaacccgcg acccccacct tggcaaggtg gtgttctacc actgaactac
     1141 gttcgcactg ttttcttcta tctaaaaatg ccggctacat gacttgaaca cgcgaccctc
     1201 tgattacaaa tcagatgctc taccaactga gctaagccgg ctcatttgtt atatcttaat
     1261 gcgggttaag ggacttgaac ccccacgccg ttaagcgcca gatcctaaat ctggtgcgtc
     1321 tgccaattcc gccaaacccg catatatgac ccgtactggg ctcgaaccag tgacccattg
     1381 attaaaagtc aattgctcta ccaactgagc taacgagtct aaaataactt gcgttacctt
     1441 aaacggtccc gacgggaatc gaacccgcga tcttcgccgt gacagggcga cgtgataacc
     1501 gctacactac gggacct
//
LOCUS       DAVHEW010000159         1514 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVHEW010000159 DAVHEW010000000
VERSION     DAVHEW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.159"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..>1514
                     /locus_tag="VVL53_002092"
     CDS             <1..>1514
                     /locus_tag="VVL53_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU4184167.1"
                     /translation="EVEDFTSETGKRKQEYDYKYDDKGNIIAYDDESALEYETEKFDE
                     IKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEIKATKYDSHSKTMTFD
                     LYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETKAEYPYVSSYGNVIE
                     LGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVTTYNPGKTDML
                     EGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQSVLMSALDGFNIIRY
                     QVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKFNVEAIKEDGSMLFID
                     TKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNESVVDNYLIYG
                     DLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSDLNAKA
                     VGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQEQH"
BASE COUNT          603 a          185 c          294 g          432 t
ORIGIN      
        1 gagaagttga ggactttaca agtgaaactg gtaagaggaa acaagaatac gattataaat
       61 acgatgataa gggaaatatc attgcttatg acgatgaaag tgctctagaa tatgaaactg
      121 aaaaatttga cgaaataaaa tcaaaaattt atggtgttct aagcccatct aaagatggac
      181 actttgaaat tcttggaaag ataagcaatg tttctaaaaa tgccaaggta tattatggaa
      241 ataactataa atttatagaa atcaaagcga ctaagtatga ctcacattca aaaacgatga
      301 catttgattt atacgctaat attaatgata ttgtggatgg attagctttt gctggagata
      361 tgcggttttt tgttaaagat gatgatcgaa taaaagctga aactaaaatt agaatgcctg
      421 aaaaaaataa ggaaactaaa gcagaatatc cctatgtatc aagttatggg aatgtaatcg
      481 aattaggaga aggagatctt tcaaaaaaca aaccagacaa tttaactaaa atggaatctg
      541 gtaaaatcta ttctgattca gaaaaacaac aatatctgtt aaaggataat atcattctaa
      601 gaaaaggcta tgcactaaaa gtgactacct ataatcctgg aaaaacggat atgttagaag
      661 gaaatggagt ctatagcaag gaagatatag caaaaataca aaaggccaat cctaatctaa
      721 gagccctttc agaaacaaca atttatgctg atagtagaaa tgttgaagat ggaagaagta
      781 cccaatctgt attaatgtcg gctttggacg gctttaacat tataaggtat caagtgttta
      841 catttaaaat gaacgataaa ggggaagcta tcgataaaga cggaaatctt gtgacagatt
      901 cttctaaact tgtattattt ggtaaggatg ataaagaata cactggagag gataagttca
      961 atgtagaagc tataaaagaa gatggctcca tgttatttat tgataccaaa ccagtaaacc
     1021 tttcaatgga taagaactac tttaatccat ctaaatctaa taaaatttat gtacgaaatc
     1081 cagaatttta tttaagaggt aagatttctg ataagggtgg ttttaactgg gaattgagag
     1141 ttaatgaatc ggttgtagat aattatttaa tctacggaga tttacacatt gataacacta
     1201 gagattttaa tattaagctg aatgttaaag acggtgacat catggactgg ggaatgaaag
     1261 actataaagc aaacggattt ccagataagg taacagatat ggatggaaat gtttatcttc
     1321 aaactggcta tagcgatttg aatgctaaag cagttggagt ccactatcag tttttatatg
     1381 ataatgttaa acccgaagta aacattgatc ctaagggaaa tactagtatc gaatatgctg
     1441 atggaaaatc tgtagtcttt aacatcaatg ataaaagaaa taatggattc gatggtgaga
     1501 ttcaagaaca acat
//
LOCUS       DAVHEW010000160         1437 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVHEW010000160 DAVHEW010000000
VERSION     DAVHEW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.160"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            432..1433
                     /locus_tag="VVL53_002093"
     CDS             432..1433
                     /locus_tag="VVL53_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4184168.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
BASE COUNT          537 a          195 c          268 g          437 t
ORIGIN      
        1 aaataatata aacacggtct cactcatata cttgttaaat gccatgatgt ttattcgatt
       61 agatgagtaa ccaaaaataa caaaaaaacg ctatttcgcg tttgaactgt acctcaaaag
      121 ttagacagaa aaaatctaat ttttggggtg tttttgttaa gaaaagacct tcaatcattt
      181 aagcaaaatg tacatctaga tttatagccc tctaaaactc ttgctttttt tgtagaagat
      241 acgaaaaaag ccataaaacc aaggcttttc ctgttttatg tagatgttta cacatgaaaa
      301 ttgaagtatt ttcaaaagct aattggagtg attttattca tgatgggata aatgatctgg
      361 ccaaaaatga ttgagaaata gttgcttaaa aagcatttgc atgatactat atattgaaag
      421 gaggtgataa tatggtagaa caaagaaaat caattaccat gaaagatgtt gctttagaag
      481 caggagttag tgttggaact gtttcacgtg taattaataa agaaaaaggc attaaagaag
      541 taactttgaa aaaagtggaa caagcgatta aaactttgaa ttacattcca gattactatg
      601 ctagaggaat gaaaaaaaat cgaacagaaa cgattgcaat cattgtacca agtatctggc
      661 atcccttctt ttcagaattt gctatgcatg tggaaaatga agtctataag agaaataaca
      721 aattactctt atgttctata aatggtacaa atagagagca agactatctg gagatgttgc
      781 gtcataataa agttgatgga gtggttgcca ttacctatag tccaattgaa cattacttga
      841 cgtcaggaat tccctttgtt agtattgacc gcacatactc agatattgcc attccttgtg
      901 tttcatcaga taatgatgca ggtggaagag aagcagctaa acaattaatt agtaaaggat
      961 gtcagcattt agcctttgtg ggcggacata atacaaccat taatgaaacc aaacgtcgta
     1021 gaatttcatt tgaaaagtat gtccaagagc ataaaatacc tcttagtatt tttgatttgg
     1081 atgagacagt tactgactat tatggaaagc tagagagttt cttggaagaa aattcttcta
     1141 tagatggaat ttttacaatc aacgatttta cagctttgga tgtgattgaa atactagaaa
     1201 aaaatggcaa acgtattcct catgatgtcc aaattattgg atacgatgga attaaaatgg
     1261 ctggagatag agattatcta ctttcaacta ttgaacaacc attggaagaa atggcaaaag
     1321 aagcggttcg tattttgttt gatataattg atggaaagac tgttaatttg cagacaatcc
     1381 taccagtaaa atttgttgaa ggaaaaacaa caaaaaatga aaataagtct tgacaga
//
LOCUS       DAVHEW010000161         1370 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVHEW010000161 DAVHEW010000000
VERSION     DAVHEW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.161"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            38..>1370
                     /locus_tag="VVL53_002094"
     CDS             38..>1370
                     /locus_tag="VVL53_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4184169.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKE"
BASE COUNT          463 a          188 c          223 g          496 t
ORIGIN      
        1 gtttaatccg ataatttata aagagagaaa gatattcatg agagatttat tatctaaaaa
       61 aagtcatagg caattagaat tattagaatt attatttgaa cataaacgtt ggtttcatcg
      121 ttctgaacta gcagagttat taaattgtac agaacgtgca gtcaaagatg atctatccca
      181 tgttaaatct gcttttcctg acttgatttt tcattcttct actaatggta tacgcattat
      241 taataccgat gatagtgata ttgaaatggt ttaccatcat ttctttaaac attcaactca
      301 tttttcgatt ttagaattca tcttctttaa tgaaggttgt caagctgaga gtatttgtaa
      361 agaattttat atcagttcat cttcgctcta tcgtattatt agccaaatca ataaagtgat
      421 taaaaggcaa tttcaatttg aagtcagtct gacccctgtt caaatcattg gaaatgagag
      481 agacattcgt tacttttttg cacaatattt ttcagaaaaa tattatttcc tagaatggcc
      541 atttgaaaat ttttcatcag agccactatc tcaattgtta gaattggttt ataaggaaac
      601 aagctttcca atgaatttgt caacgcatag aatgctaaag ttgctcctag ttacgaacct
      661 atatagaata aagtttggtc attttatgga agtagataaa gattctttta acgaccaaag
      721 tttggatttt ttaatgcagg cagaaggaat agaaggtgtt gctcagagtt ttgaatcaga
      781 atacaatatc tctttagatg aagaagttgt ttgccaatta tttgtgtctt actttcaaaa
      841 aatgtttttc atagatgaaa gtctctttat gaaatgcgta aaaaaggata gttatgttga
      901 aaaatcttac catctattga gtgattttat tgatcagatt tcagtcaagt atcagattga
      961 aatggagaat aaggataatc tgatttggca tctgcataat accgcacatc tgtatcgtca
     1021 agagttgttt actgagttta ttttatttga tcaaaaaggg aatacaatca ggaactttca
     1081 aaatattttt ccaaaatttg tttcagatat aaaaaaagag ctttctcact atttagagac
     1141 tcttgaggtt tgttctagtt caatgatggt aaatcatcta tcttatacct tcatcactca
     1201 taccaaacat ttggtgatta atttgctaca aaatcagcct aaactaaagg ttttagttat
     1261 gagtaatttt gatcagtatc atgcaaaatt cgttgcagag acactttctt attactgtag
     1321 caataatttt gaactggaag tttggactga attagaatta tcaaaggaat
//
LOCUS       DAVHEW010000162         1298 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVHEW010000162 DAVHEW010000000
VERSION     DAVHEW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.162"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..282)
                     /locus_tag="VVL53_002095"
     CDS             complement(<1..282)
                     /locus_tag="VVL53_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4184170.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFF"
     gene            complement(270..827)
                     /locus_tag="VVL53_002096"
     CDS             complement(270..827)
                     /locus_tag="VVL53_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU4184171.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            complement(728..1285)
                     /locus_tag="VVL53_002097"
     CDS             complement(728..1285)
                     /locus_tag="VVL53_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU4184172.1"
                     /translation="MFGVGMMGLIPILFLIYPTVLAIVAGTVVMLFMAKVQKPWALFI
                     FGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRKIGNYNSFKYNMLSYAIFSTWICSS
                     LMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYPHMALVALGAFLGGILGAYIGKALL
                     KKHFSNGLYCVGYFTPCLILWCYLN"
BASE COUNT          448 a          249 c          175 g          426 t
ORIGIN      
        1 aaaaaaatat gtcttaggat tttgaaaaac agttgaaaca agcattgaga tttgataaag
       61 ttccaaattt tttatattct taccatcaat aattatttcg ccctttgtct ttgcgttatc
      121 ataccttaca gctagtccat tgattgaatt tattaaagat gacttaccat ttccactttt
      181 tcctgtaaat agcacgcact ctccctcggc tatggatagt gatatatcat ctaatatatg
      241 ctcttcacca taatcaagac ttaaattttt tatttcaacc atattaagcc tcccacaata
      301 agtccagcaa ccgctaaaac ataaacaaaa tcaattagct gtatctttac gcgaatgtat
      361 ctggtcttgg caattggatt ttctattgcc tttgtttctg ccgcttttgc aatgtcatct
      421 gatatattag atgatataat gagtagtggc acagaaacat attcaagata tttgactggg
      481 tttttaaaat ttatccctct tactctcata gccattttga tgtttttctt ctcctcctta
      541 aaagatggga agaatctaaa cataaccgca ataggtatgg atacattctt tgaaatctta
      601 agcttatcca tcgatgaaat tattgcgcct acatccgagg tctttatcat aaagcttgct
      661 gccataaatg gtaaaataaa cattctaata acaataggta tactcaaaaa catcttaact
      721 atagggttta attcagatag caccataaaa ttaggcaagg agtaaagtat cccacacaat
      781 ataatccatt tgaaaagtgt tttttcaata gagccttgcc tatatatgct ccaagaattc
      841 ctcctaagaa agcacctaag gctactaaag ccatgtgagg ataagttatt aacttttcta
      901 atacatcaac ataatctttt cccatagtca tcaaagacca ctccatatat ttttcttttg
      961 ctaaaagcat ttgcattaaa gagctacata tccatgtgct gaagattgca taagaaagca
     1021 tattgtattt aaatgaatta taattaccaa tctttctaat taattctgct attatcatta
     1081 ctataagtga taaaaccaca actacgtagg tatgaccagc tgcaaacatc acaagtggtg
     1141 atatcatacc aaatataaat agtgcccatg gcttttgaac cttagccata aataacataa
     1201 caacagttcc tgcaactatg gctaatactg tcgggtatat taagaacaat attggaatca
     1261 agcccatcat accaactcca aacatgaagg caaaataa
//
LOCUS       DAVHEW010000163         1279 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVHEW010000163 DAVHEW010000000
VERSION     DAVHEW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.163"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            82..1260
                     /locus_tag="VVL53_002098"
     CDS             82..1260
                     /locus_tag="VVL53_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU4184173.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          475 a          236 c          184 g          384 t
ORIGIN      
        1 ttaaaaagat taacatctac atttatccat agcaaaaaat cataggattt taagaatctg
       61 acaactagga agaaaactaa tatgacctta tctaagaaac aattacaact aagagctaaa
      121 atactggaaa cagtttacac attaggtccc atttcacgaa ttgaaatcgc tactaaaaca
      181 ggaataacac ctgcaacaac tagcagtatc acaaatgatc tgattaaaga aaatattctc
      241 ttagaacttg gtgaagacga acacgataca agcgtaggga gaaaaaaaat cctgctagac
      301 attcaagcta aacgatttta ctacattggt tgtgaactat ctgaaaaaca tttcactttt
      361 gctttaggag ataatctagg aaatatctta aaggaagaga aagaaattgt tactaaacag
      421 ttaatccaag aaaagggaaa tcaactcatt aaccagacac taaagcaatt cttaaataac
      481 tgctctgatt atgaaataga agcaattgga atcgcgctac ctggtcgcta tttagataat
      541 tataaaataa caacaaacaa tcctttatgg caacatatcg acttagaaat gattcaatcc
      601 cactttgata agcctttgtt tttctctaac aatgttaact gtatggctat aggtaaacgc
      661 ctatttagtc gccaacagaa tgaccctaat tttgcttatt ttcattttgc tagaggtatg
      721 cactgttctt atatctacga cggaaacatt tatgggaaag gaaatctaat gattggagaa
      781 atagggcata cagttgtttc atctgaggga gaagaatgta gctgcgggcg caagggatgc
      841 ttacaaactt ttgctggaga atcttggtta atcaaaaaat caaaaattct atatcatcaa
      901 tctccctatt ctctacttcc tagtttagtc aaaaatgccg atgatattga tatccaagtt
      961 attttaactg cttatcaatt aggtgacact ggaataatca cacttatcca tcaagcctta
     1021 ctctatctta gtcaaaccat tttaaatatc agtatgatga ttgactctca aaaaatctac
     1081 ctacatagtc ctttactaac aaatcaacac atcatccaaa aactatatag tgaaatgaac
     1141 tacaaaccca aactgctcta taatcgccta cctgaagtca ttattgaacc ttacaatgat
     1201 tttacagccg cacactcagc tattgcactt tgtttgtatc atacaattct acattcttaa
     1261 ttttctatta catttaata
//
LOCUS       DAVHEW010000164         1278 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVHEW010000164 DAVHEW010000000
VERSION     DAVHEW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.164"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..>1278)
                     /gene="pfbA"
                     /locus_tag="VVL53_002099"
     CDS             complement(<1..>1278)
                     /gene="pfbA"
                     /locus_tag="VVL53_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU4184174.1"
                     /translation="NEVFSIRKLKVGTCSVLLAISILGSQGILSDEAVTSSSPMATKE
                     SSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNSQLDNRT
                     VESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAIQDAIDA
                     AAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIVFMTGLF
                     TDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNSNNVTIK
                     NVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEPLTRKGF
                     PYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKILNNHFDN
                     MMYSGVRFTGFTDVLIKGNRFDKKVKGESVHYR"
BASE COUNT          392 a          252 c          178 g          456 t
ORIGIN      
        1 ctcgataatg tacactctct cctttaactt tcttatcaaa gcgatttcct ttgattaata
       61 catcagtgaa tcctgtaaaa cgtacacctg aatacatcat gttatcaaaa tgattattta
      121 aaattttaat attagagggg ttctgtgtcg acaatgtttg atagtgtgtg ccaattgctg
      181 ttactaattc cccagattta tcacttttgc caaaatagga attttgaata gtcacatttt
      241 cagatttttt cccatcatca ttcaaggcat aaggaaaacc ttttctagtt aatggttcaa
      301 tctgaatgct ctccttactt atgatttgcc catccttcat cgttttgggt aaggcttgcc
      361 caagaaaacg agaattatca actaatacat ttttcgaacc tgcaatttga atagcatgcc
      421 cttgataact atccttgaat gttacatttt ttatagttac gttatttgaa ttcccaatag
      481 caaatgcacc tgaagaattt ataagtggta gattttttgc tttagttcct tcttcattca
      541 aagcaccgtt catatcaatc gtaccaccag aataactaat atcttctgtt gggccccatt
      601 ctacttgcgc accatcatcc gtaaataaac ctgtcataaa aacaatggaa gggtgattct
      661 taatatttat accatttaga attgtagctt tctcattcaa ttctaagtgt gtatgacttt
      721 ttaaaaaaac aatttctttt actaaataag ttccttcagg aaaatataca tttcctccac
      781 ctagcccttg agctgcagca tctattgcat cttgaattgc ttgtcgatca tcatgaatcc
      841 catcacctac cgcaccataa tcttttacac ttaaagcagt atcttcaaat tcttttttga
      901 taattctgtc acttataaca tcttccttat aactcttatt ttcattagta gatgttactg
      961 tagattcaac tgttctgttg tctaattgtg aattggaacg aatagtacca ttagagctga
     1021 tactattaac acttaacgaa ttatctaaac cattagtggt tgaattagag gcaatcattt
     1081 cagcagaacg attctgatta acagttggtg aattatctaa atcattagta attgcattag
     1141 aagactcttt tgtagccatc ggtgaagaac tagtaacagc ttcatccgat aaaatacctt
     1201 ggcttcccaa aattgaaatt gccaatagta ccgagcaagt ccctaccttt aatttacgaa
     1261 tactgaatac ctcattag
//
LOCUS       DAVHEW010000165         1260 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVHEW010000165 DAVHEW010000000
VERSION     DAVHEW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.165"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            596..835
                     /locus_tag="VVL53_002100"
     CDS             596..835
                     /locus_tag="VVL53_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184175.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLHKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            complement(850..>1260)
                     /locus_tag="VVL53_002101"
     CDS             complement(850..>1260)
                     /locus_tag="VVL53_002101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184176.1"
                     /translation="LQKIDKDTTFALFDSLFKHSIIFLHSYKNISDERACFLISLNNK
                     KRKLKPADNLYSHFISFTSVLRTLAYFLALVKCRVTGLNTPLLSLRKPKTADLLFLSS
                     KNMTSNFIVFLDKSLATMQQLASLDIEDIADKLT"
BASE COUNT          419 a          168 c          240 g          433 t
ORIGIN      
        1 tatgtgtagg actagcccct ctttttcata tatatgtcat aggttcggaa ctgagttttg
       61 tgaaaatagt gttatctatt ttgggagtta tatttgtaag tatgttaact attgctcgaa
      121 tttatcggac ttttctataa agaatagttt ggacttgaac tgtatctaaa aacagaggtt
      181 ggtagattat cagcctcttt cttgtcgttg aaaagagtga gatatattga cttttgaaaa
      241 aaatgtcata attgagttgg gataaaccta aaagtaatat agtagattga atctggaata
      301 gtccgttatg actgttaaaa tatttctaga aattaatttg actttcctaa tcgatttgtt
      361 cacatcttgt ttcaatctac tatactttct cgttaaaccg ttcggtaatt catggtgaca
      421 tggacacata gtagtaagca cactatgtgg cttggcagat ctaaaaactg ttcccttgcg
      481 ataagcctaa taagcacaac atagggagtt agagaagccg actctaatca accactttgg
      541 gcagtagtgg taactgcccc gtgcttttta ttttgagaaa atatggagtt tgtcgttgaa
      601 attacttgat tgtattttgg attatcaaga aagattcaat ggaaaaacat gtcaagtatc
      661 aacgaattat aagtatttag agattttcaa agtgaatttt tgcttgactg atttacatca
      721 tttatttgac ttacacaaaa tcacacgaga ttatgctagt caaacaaaac ctgctattca
      781 agatggtgtt tttattttag aagattttag aaatatccta tgtacaatga tgtaatcgaa
      841 aggatatctt tatgtgagtt tatcggcgat atcttctata tctaagctag caagttgttg
      901 catcgtagct aaggatttat ctaaaaatac tatgaaattg gacgtcatat tttttgagga
      961 taaaaataaa agatccgcag ttttaggttt acgaagagac aaaagcggag tatttaaacc
     1021 agttactcta cattttacaa gcgctaagaa atatgctaaa gttcgtaaaa cagatgtgaa
     1081 agaaatgaaa tggctataaa gattgtccgc aggcttaagc ttgcgttttt tgttgtttaa
     1141 cgaaattaaa aaacaagctc tctcatcgga aatgttttta taggaatgaa ggaagatgat
     1201 gctatgttta aaaagagaat cgaaaagggc aaaagtagta tctttatcta ttttttggag
//
LOCUS       DAVHEW010000166         1247 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVHEW010000166 DAVHEW010000000
VERSION     DAVHEW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.166"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..507
                     /locus_tag="VVL53_002102"
                     /pseudo
     CDS             1..507
                     /locus_tag="VVL53_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            407..853
                     /locus_tag="VVL53_002103"
     CDS             407..853
                     /locus_tag="VVL53_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4184177.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKTVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHPDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
BASE COUNT          424 a          229 c          206 g          388 t
ORIGIN      
        1 gaaaaagtag ccttatttct taagaatttt aatagtttaa agcacctaac acctgtttag
       61 attgacgaaa caggattcga tacttatttt tatcgagaat atggtcgctc attaaaaggt
      121 cagttaataa gaggcaaagt atctggaaga agatatcaga ggatttcttt ggttgcaggt
      181 ctaacaaatg gtgaattaat cgctccaatg acttacgaag agacgatgac gagcgacttt
      241 tttgaagctt ggtttcagaa ttttctctta ccaacattaa ccacaccatc ggttattatt
      301 atggataatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
      361 tataaacttt tacctcttcc tccctactca cctgagtaca atcctattga gaaaacatgg
      421 gctcatatca aaaagcacct caaaaaggta ttaccaagtt gcaatacctt ttatgaggct
      481 tttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      541 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaacag ttcatagttc
      601 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      661 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      721 tttaaaaaca atcacggaca gggttggtca tcccgactct gaagtcacta cttccatcta
      781 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
      841 aaagattttt taaaaagttt tgtccctttt ttgccctcta aatacaaaaa tagcccttcg
      901 gataaaatcc gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg
      961 gttcgggata aaatagttca ctgaactatt ttatttttta aggttatcat aatatcaaat
     1021 agttcaatta aatacgctaa attactaata tactttttac ctttttcatt ctaaaatgta
     1081 aagtacaaac aattacaata tactagaggg ggagtaaaaa aggtattaaa tcgatgagtt
     1141 cagcaggcaa gaaaatagca cctttacggg tgctattttt taattaacgc cacgttaact
     1201 tttgattgat gaattttatt gtttggcact tctttcattt cacggta
//
LOCUS       DAVHEW010000167         1230 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVHEW010000167 DAVHEW010000000
VERSION     DAVHEW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.167"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            36..>1230
                     /locus_tag="VVL53_002104"
     CDS             36..>1230
                     /locus_tag="VVL53_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU4184178.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHS"
BASE COUNT          443 a          236 c          180 g          371 t
ORIGIN      
        1 atgtttaata taaaataaac caaaggagga aacaaatgat tacaaaacaa tcagcctttt
       61 tacaaaatag agccactata ctggagtttc tatatcgaaa tccagcaaca tcccgtacag
      121 atattgttaa tgaaacagga cttactccag caacaacgac taacatcata aaagagctaa
      181 gcgaacagtc cctcatatac gaaactggtg atgaatttag cgagttttcc ggatctggaa
      241 gacgtagaaa aactatttct atcacagata atattccgta tgttgttgga ggtattgaaa
      301 taaacgtcct aggtatcttt ctcagtctat gtgacttaca agggaaaact cttttcgaga
      361 cagaaatttt gaatgaagat tatcctattt cagaaatcaa ttccaccatt accaatatga
      421 taaaaacagc tatagagtac gtccctttgg aaacaaaatt acttggattt ggcttatcaa
      481 tacctggaca ttataacaaa gactccggaa gtatcattac aaacaacccc atatgggaat
      541 cttttaattt attaaatgta attaaaagat tcaattttcc ttttattgta aaaaataata
      601 tcgattgtat ggctatagga caataccttt ttaatccaca caataccccc gataacttta
      661 ttttcctaca cgctggatta ggtatttaca cttccttttt cacaaaagaa aaaataggag
      721 cctctaaaaa tccttatatc ggagaaattg gacacaccat tgtcgaattg aatgggcaat
      781 attgtgaatg cggaaaaaaa ggttgtttac aaacatatat ttcggatgct tggttaatca
      841 aacacgccca attattattt aaaaattccc aactaactgt actaaaaagc cttgtaaaga
      901 ctgaaaaaga cattcattta gacacccttt taacggctta taatttaggc gactccgctt
      961 tacgtcaaca aattgataaa ggagtcaatt tattagccac ttctattgca aatctcctcc
     1021 tcatcaatcc tgctgataaa atctatatca acagtcaatt gcttaattat caacctttca
     1081 ctcatgaagt cagggataaa atccaagacc agctccactt cgttcccttt actcgtaata
     1141 tagaaattga aattttacct tacaacaaac atcgtggaag tataggagct tgtgcattag
     1201 ctatcgtcgc ttttttcata gaacatagca
//
LOCUS       DAVHEW010000168         1191 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVHEW010000168 DAVHEW010000000
VERSION     DAVHEW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.168"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..1181
                     /locus_tag="VVL53_002105"
     CDS             <1..1181
                     /locus_tag="VVL53_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU4184179.1"
                     /translation="RYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGG
                     QLASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQ
                     EGVAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLL
                     FRMDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYS
                     ETLYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENF
                     IKEMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLF
                     LHVVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH
                     "
BASE COUNT          329 a          274 c          232 g          356 t
ORIGIN      
        1 gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt caactgttaa
       61 caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac tttccaaaat
      121 tgttggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt tccagaactg
      181 acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa ttctttttac
      241 agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc acaacttatg
      301 gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat catcctcttt
      361 atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct ggtaatcgtt
      421 attgttctga agaggcagac agctttatca cacctgtttt agaacggttt aatcaacttc
      481 tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt gaaaaaacag
      541 ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt ggagaccttt
      601 ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc tactcagaaa
      661 ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc tctgaacgaa
      721 aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg agtggaacaa
      781 gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc atcaaggaga
      841 tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa aacgaagttc
      901 gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat ctagctggag
      961 gtgacttcca aactttaaca atcaaacgct tccgccatct ttttcttcac gtggtgggaa
     1021 aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc tagtctctat gcctattccg
     1081 aattgttttc agcactttat tctaggatta gaaaagtcaa cctgaatctt cctgttcctt
     1141 atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta aagaacagtc g
//
LOCUS       DAVHEW010000169         1082 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVHEW010000169 DAVHEW010000000
VERSION     DAVHEW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.169"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            27..758
                     /locus_tag="VVL53_002106"
     CDS             27..758
                     /locus_tag="VVL53_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184180.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            773..>1082
                     /locus_tag="VVL53_002107"
     CDS             773..>1082
                     /locus_tag="VVL53_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4184181.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESVRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     D"
BASE COUNT          405 a          142 c          209 g          326 t
ORIGIN      
        1 aaaaagtatt tggaggagga agaattatgt tgaatcagga tctctttgat tcgcttgagg
       61 cacaaaaaat tgtagatact ttgatgaaag ggcaaaaaga ttatgtagat gaacgtctag
      121 aaaaaagaga gacaatgata gtgtcgaatg gttatgcatg gacgcgacct aatcatattg
      181 atactgcatt tgcatcagca gatttgtttg agtataaatt acaattagca ggacagactt
      241 ggggatattt agaatttgaa acaaatacag aaaaatatgg gaaagtattg ttaattataa
      301 agggtaagaa gcgacttacg aaccaatttc ctttggtaca aaaaaataag agtggctact
      361 tatttgaata cgctcagatg aatacacttt atcttaatca acattcttcc tacaaaaatg
      421 atgaagatag tcattccttt ccaattcaga tggagttagt ttctgatgaa atgattcaag
      481 aaattgaaca agctactaaa aattcgaata tcgaaaaatt tatgatttta acttatgagg
      541 cggactcaga aaacaatatt atatctgtag atgttgttat gcctgatgca cgaactggtc
      601 agttacactt gattcaagat ttgtctgagt atattcaatc aagttcgtac catttcgaag
      661 aagctaaata tcaagatatt cctaattttt cagaattatc ggaaacagaa gattttgaaa
      721 ttattccaag aatagaaaaa caagaaggtc aaaagtagta aggagtatgt aaatgtttaa
      781 tggtcgggta ttgaaagaat tacggctgtt aaatggttta agtagagcag aattagctca
      841 gagaattaat ttaacggaac aagccatttg gcagtttgag tccaacgaaa cgaaacctaa
      901 attatcaacc aaaatgcatt tggccaacca atttcatgtt gatttaactt attttgaaca
      961 ggaagaagag agcgttcgat ttgattcttc tgtaattgcc tttagaaatg cagacctagc
     1021 aacacggaaa acaatagata ttcaaactat gtatttacat aaggtagata gtttgattga
     1081 tt
//
LOCUS       DAVHEW010000170         1056 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVHEW010000170 DAVHEW010000000
VERSION     DAVHEW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.170"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(225..428)
                     /gene="blpN"
                     /locus_tag="VVL53_002108"
     CDS             complement(225..428)
                     /gene="blpN"
                     /locus_tag="VVL53_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU4184182.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(444..698)
                     /gene="blpM"
                     /locus_tag="VVL53_002109"
     CDS             complement(444..698)
                     /gene="blpM"
                     /locus_tag="VVL53_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU4184183.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(848..>1056)
                     /locus_tag="VVL53_002110"
     CDS             complement(848..>1056)
                     /locus_tag="VVL53_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4184184.1"
                     /translation="RKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKMFSTTY
                     RNHRKRFGLRMNLSAGIINHELGF"
BASE COUNT          336 a          227 c          143 g          350 t
ORIGIN      
        1 ttacggaaca agtttaatat aaaaattatc aaaaaaacat aggcaataaa gagaaaaatt
       61 aatttatcat agattagaaa taatatgaca aaacaattca atgatgttaa ttcaatagtc
      121 ttttgttttt tatcggagat acttatggat agataaataa gataggtttg aaaagcgaag
      181 agaataataa agaatatagc cttcataaaa tttagctttc atttttatga tgtagcggta
      241 taggctaaat atccacaaac tactgctcct ccaattcctc ctattgcagc gccccatggt
      301 cctagaagtc tcccatattt cactccaccc gctgcacaac ctaaagcagc aactacagct
      361 gctcctccgg aattacctcc ataaacctca ctcagcattg tttcatttat attacaataa
      421 gtattcatac aagtctcctt ttattaaaat ccacccgttg cccctgttac tcctgcccaa
      481 agatccacac caaatttagc tcctatgtat ccacatgctc ccataaatgg tgctccaaca
      541 ccactcgcag cacaaatagc tgtccctagc ccccagccac caaaagcagc accaccacct
      601 tctaagacat tagtttgcca attattcttg cctccttcaa tactagataa catagttata
      661 tccatttcat gaaattgttc cataattttt gtatccatga caaatactct tttttatttt
      721 taatttttgt cttgttgtaa ctttgacaag tttagtatat catcgttttt taaaattttt
      781 catccagatc ttgaatagtc atcgaaacgt cttgaattgc aaaaattaca ttagatttcc
      841 tgcaaaacta gaatcctagt tcatgattga taataccagc actcaaattc attcgtaatc
      901 cgaagcgttt acgatgattt cgataggttg ttgaaaacat tttaaacgtt tttactttgg
      961 caaagatgtt ctcaaccttg cttctctcct tagatagcgc atggttacag gctttatctt
     1021 caactgttag cggtttgagt ttgctggatt tacgtg
//
LOCUS       DAVHEW010000171         1048 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVHEW010000171 DAVHEW010000000
VERSION     DAVHEW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.171"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(351..923)
                     /locus_tag="VVL53_002111"
     CDS             complement(351..923)
                     /locus_tag="VVL53_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184185.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAVWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT          339 a          175 c          173 g          361 t
ORIGIN      
        1 tcaggagata gggtgagtcg acatgtctat tactcaccca aagcagtcct acaaagcagg
       61 aattttctgt tacttttttg gaaatagtaa cgtttataca gctttgacac ttcgtatcaa
      121 agcgccaaac acactccgag ggatttacag aaagcagaaa aggaatgatc tggtataaga
      181 tcattccttt tctctctttt tctttaagta attatataca atgtacgacg aagtcgtcat
      241 tgcaatgctg atccaccacc taaagggaac tttaaacaac attgataaga taaagaatat
      301 aaacaacgaa aatacgttat acccaattaa ttttattgta tatctcatga ttaaaagtta
      361 atccttccgt tgttaggaat ggcatcattt ttatcccata attgtgctaa ataagtcccc
      421 ggtgataata aattcatagc gaattctaaa gcaacatcat ttacaaacca actacctaga
      481 tatctagaaa ttgctgaacg aatagcactt tttgctgcat gttttccttt tactttaatt
      541 agatttgcaa ggcctgcagt agttcctcct aatgctaaag ctattgcagt atctaataga
      601 gcacccattt gattaactgt aataccttgc caaactgctc taaatggaga gtatgtaggt
      661 gggattgtat aatcgccttg taattgtcgg ttaattactt ctttgatcca ttgttgtgag
      721 acgtctggat gaaaagattg gatttcgttt gcaagtgtat tgatttgttc ttctgttaga
      781 gaagtgacag gttgaagttc catatttgtt tcaatttgtg atacttgttc agaagcgtat
      841 acagctgaaa cacttggaat cgctgataca attaacacaa ttgacgtcaa aaaaaccgaa
      901 ataaatttca ttaatttgtt catgagcttt tctccttttt atttgcatct gcttacattt
      961 tatcatatac tgttattata gtcaaaaaaa tatgctatta tgttaaaaaa atatttttca
     1021 aaatataaat ggacggattt attttgga
//
LOCUS       DAVHEW010000172         1025 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVHEW010000172 DAVHEW010000000
VERSION     DAVHEW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.172"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            160..459
                     /locus_tag="VVL53_002112"
     CDS             160..459
                     /locus_tag="VVL53_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU4184186.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            640..>1025
                     /locus_tag="VVL53_002113"
     CDS             640..>1025
                     /locus_tag="VVL53_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4184187.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIVNKDTHKEIIAKLDYEAPSCP
                     ECGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKIMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLS"
BASE COUNT          377 a          198 c          172 g          278 t
ORIGIN      
        1 aactctcacc aataaaagac tatgtcttaa aaaaatggta taataaaatc aatacttggg
       61 cttgatggct atgctactaa taacaattag gagagaaaat caggcacttg ttaacaacaa
      121 ggattatccc cttgagatga aaggaacttt agaaatctta tgatgaacat gcaaaacatg
      181 atgcgtcaag cacaaaaact tcaaaaacaa atggaacaaa gccaagctga acttgctgct
      241 atgcaatttg ttggcaaatc tgctcaagat cttgtccaag cgaccttaac tggcgataag
      301 aaagttgtca gcattgattt caatccagct gtcgttgacc cagaggacct tgagactctt
      361 tctgatatga ccgttcaagc catcaactct gctcttgaac aaatcgatga aactaccaag
      421 aaaaaactgg gtgctttcgc tgggaaatta cctttctaaa aacaaggagc tagaacaatg
      481 cttgtcgata acaaaggcta agaaaggtgc aaaaatgact ctataatatt tgtagtgggt
      541 aaatccccta tggatattat ggagcctatt tttgtgtaga aaaaagtccc atatgaccta
      601 taatgaaaag cgacaaaaca actcattaga aagaatcata tggaacaatt acattttatc
      661 acaaaattac tagacattaa agaccctaat atccagattt tagacatcgt caataaggat
      721 acacacaagg aaatcatcgc caaactggac tatgaagctc catcttgtcc tgagtgcgga
      781 agtcaaatga agaaatatga ctttcaaaaa ccttctaaaa ttccttatct tgaaacaact
      841 ggtatgccta ctagaattct ccttagaaag cgtcgtttca agtgctatca ttgttctaaa
      901 attatggttg ctgaaacttc tatcgtcaag aagaatcatc aaatccctcg tatcatcaac
      961 caaaagattg ctcaaaagtt aattgaaaag atttctatga ctgatattgc ccatcagctt
     1021 tccat
//
LOCUS       DAVHEW010000173         1022 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVHEW010000173 DAVHEW010000000
VERSION     DAVHEW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.173"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..136)
                     /locus_tag="VVL53_002114"
     CDS             complement(<1..136)
                     /locus_tag="VVL53_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU4184188.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     A"
     gene            complement(148..714)
                     /gene="amaP"
                     /locus_tag="VVL53_002115"
     CDS             complement(148..714)
                     /gene="amaP"
                     /locus_tag="VVL53_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU4184189.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(798..962)
                     /locus_tag="VVL53_002116"
     CDS             complement(798..962)
                     /locus_tag="VVL53_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU4184190.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAEMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT          346 a          200 c          150 g          326 t
ORIGIN      
        1 cagctgcaac tcccagtgct cctaaaatca atacaaatat tgttttgaag aagccaaagg
       61 agacaatcaa acaagccaga aatacgccta tgagaccagc gataattgga tatcgatatt
      121 gtttaagcca ttccattttt tacttcctta cttcacacga ctaacagtct ttttgttttg
      181 aggttttggt tggtaatttt ttactgcaac ttcaagtttt acttgacgct caataccaaa
      241 aaactgcttc aatccattag ttatttcatt ttgaattatt tggcatctgt cagcgatgtt
      301 gtctgaagga agaattttac cttctacatg aacgaaacat ttattttttc gtaaatttac
      361 atgaacagtt gggttcttga tcaatctatg atcactcacc aaacttctaa caaaaccttc
      421 gattgccgaa ttctttaatt ttaatgtatc gtttttagtt tcaagttgga tttccaagta
      481 acgtttagga taaaacatca caactaaaat ggataataaa actaaagttg atagaaccac
      541 tgtcccccaa aagacatatc tagcaagata gaattcaact acggagttct gtctccagct
      601 aagtagatga atacctagat cactaacttg atgataatct atcaaaacag gaaggaaaat
      661 tgtcaagatt aaaatacaga aaataatgaa acatattttc tttgcttttg acatatacaa
      721 tcctttcgat ttaacattta aactattttt cccgtcattg atttaaaaat agtttgctaa
      781 ctttactggc gaaaagttta ctttctacct gtaaagaatg acactacagt aatcacaatc
      841 gctgcaccta caatagatgg gagcaaagcc atttcagcga ttgatggacc ccaactacct
      901 aaaagagatt gtcctgcata tgccccgact aaaccagcga atacatttgc gatgattccc
      961 atagaactac ttttttagtg attttacctg ccaagagacc aataagacct ccagcaataa
     1021 tt
//
LOCUS       DAVHEW010000174         1021 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVHEW010000174 DAVHEW010000000
VERSION     DAVHEW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.174"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            170..967
                     /locus_tag="VVL53_002117"
                     /pseudo
     CDS             170..967
                     /locus_tag="VVL53_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          355 a          203 c          156 g          307 t
ORIGIN      
        1 aagataaaaa taggaaaagt tgacaaaata gaaacaaatt tgctacctaa atttcaaaca
       61 attaaagttc taaaaagacc gccttaaaac ttttgggtaa atcctattag aactataata
      121 tttttatata ggtattatgg ttctgatttt ctagtataaa aaaattcaaa ctagaattct
      181 tcttagaaaa ggccgtttta agtgctatca ttgctcaaaa atggagctcg ctgagacttc
      241 tatcgtcaag aagaatcatc aaattccttg tatcatcaac caaaagattg ctcaaaagtt
      301 aattgaaaag atttctatga ccgacattga tcatcagttg tctatttcaa cttcaactgt
      361 cattcgcaag atcaataatt ttcactttga gcatgatttt tcgcgtcttc ctgagattat
      421 gtcctaggac gttgaaacag tccggggagt gactgtttca atcgggagat ggagatgagc
      481 tttattgctc aggactttga taaactcaat atcataactg ttcttgagag cagaacacaa
      541 gccatcatcc gaaatcccat gaatacaagg ctatcaagcg ctactggaag ctcattcaac
      601 aagatagtca gaaactgagt gataaacgat tttatcgccc tacttttcgt atgcacttga
      661 ctaataaaga aattctagat aaacttttga gctattccga agactttaaa caccactata
      721 atctctatca gctcttgctt ttccacctct gtttcagact ttgtttaaaa ccttcctcaa
      781 agataaagag aaaatcgtca acgcccttca attaccttat tccaacgcca aactggaagc
      841 caccaataat ctcatcaaac ttatcaagca caatgccttt ggttttagga actttgaaaa
      901 cttcaaaaaa gaaaggacga aatttgtcct ttctagatct tcgctttctt caacccacta
      961 cagttgacaa agaaccgaaa aatgtctcgc ctctaatata gtcaattgaa acaagagcag
     1021 g
//
LOCUS       DAVHEW010000175          986 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVHEW010000175 DAVHEW010000000
VERSION     DAVHEW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.175"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            40..>986
                     /locus_tag="VVL53_002118"
     CDS             40..>986
                     /locus_tag="VVL53_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4184191.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALY"
BASE COUNT          318 a          135 c          185 g          348 t
ORIGIN      
        1 ttatatctaa aagggatatt aaagaaagga gatatgctta tgaagattta caaaaaacta
       61 tttgcttatg tccaagataa gaaatatctt ggggttttgg ccataatttt ttctgctata
      121 tctgctgcac ttacagtata tggatattat ttaatctaca aatttctaga taagttaata
      181 attaattcaa acttatccgg tgcagagagt atagcattaa aatctgttat tacactaaca
      241 agtggagcga tattttattt tgtctcagga atgttttcac atatcttggg attcaggctt
      301 gaaacaaatt taagaaaaag gggaatcgat ggtctggaaa aagcaagttt taggttcttt
      361 gacttaaatc catctggtca aataagaaag attatagatg acaatgctgc acaaactcat
      421 caggtggtag cacacatgat tcccgatagt tctcaggcaa taatcacacc cgtacttgta
      481 cttgcacttg gctttatagt aagtataaga gttggcataa ttttgcttgc tcttactata
      541 attggtggct taattttagg ggcaatgatg ggcgagcaag aatttatgaa gatataccaa
      601 gaatccctat ctaaactaag tgctgaaact gttgagtacg tgagaggaat gcaagttgta
      661 aaaatattta aagcaaatgt agagtctttt aaaagctttt ataaggcgat aaaagattac
      721 tcaaagtatg cttatgatta ttccctatct tgtaaaaggc cttatgtttt gtatcaatgg
      781 ttattttttg gactgattgc aattttaatt attcctatag tttattttat gactagctta
      841 gctagcgcaa aggtgatttt acttgagctt atcatgattt tatttttatc aggagttctc
      901 tttgtttcat tcatgagaat gatgtggtac tccatgtata tttctcaagg aaattatgca
      961 gtagatactt tagaggcgct ttacga
//
LOCUS       DAVHEW010000176          955 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVHEW010000176 DAVHEW010000000
VERSION     DAVHEW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.176"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..>955
                     /gene="pcpA"
                     /locus_tag="VVL53_002119"
     CDS             <1..>955
                     /gene="pcpA"
                     /locus_tag="VVL53_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU4184192.1"
                     /translation="AAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLT
                     VEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDET
                     GRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIEL
                     PASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGSN
                     LFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELAS
                     YSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLE"
BASE COUNT          365 a          140 c          162 g          288 t
ORIGIN      
        1 gctgcagtta ttttagcagc atatgtccct aatgaaccaa tcctagcaga tactcctagt
       61 tcggaagtaa tcaaagagac taaagttgga agtattattc aacaaaataa tatcaaatat
      121 aaggttctaa ctgtagaagg taacatagga actgttcaag tgggtaatgg agttactcct
      181 gtagagtttg aagctggtca agatggaaaa ccattcacga ttcctacaaa aatcacagta
      241 ggtgataaag tatttaccgt tactgaagta gctagtcaag cttttagtta ttatccagat
      301 gaaacaggta gaattgtcta ctatcctagc tctattacta tcccatcaag cataaaaaaa
      361 atacaaaaaa aaggcttcca tggaagtaaa gctaaaacta ttatttttga caaaggcagt
      421 cagctggaga aaattgaaga tagagctttt gatttttctg aattagaaga gattgaattg
      481 cctgcatctc tagaatatat tggaacaagt gcattttctt ttagtcaaaa attgaaaaag
      541 ctaacctttt cctcaagttc aaaattagaa ttaatatcac atgaggcttt tgctaattta
      601 tcaaatttag agaaactaac attaccaaaa tcggttaaaa cattaggaag taatctattt
      661 agactcacta ctagcttaaa acatgttgat gttgaagaag gaaatgaatc gtttgcctca
      721 gttgatggtg ttttgttttc aaaagataaa acccaattaa tttattatcc aagtcaaaaa
      781 aatgacgaaa gttataaaac gcctaaggag acaaaagaac ttgcatcata ttcgtttaat
      841 aaaaattctt acttgaaaaa actcgaattg aatgaaggtt tagaaaaaat cggtactttt
      901 gcatttgcag atgcgattaa acttgaagaa attagcttac caaatagttt agaaa
//
LOCUS       DAVHEW010000177          937 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVHEW010000177 DAVHEW010000000
VERSION     DAVHEW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.177"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..>937
                     /gene="phtB"
                     /locus_tag="VVL53_002120"
     CDS             <1..>937
                     /gene="phtB"
                     /locus_tag="VVL53_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU4184193.1"
                     /translation="IDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSK
                     LAKQESLSHKLGTKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLE
                     RLKDVSSDKVKLVEDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGY
                     IFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDS
                     GNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFD
                     EGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          304 a          180 c          210 g          243 t
ORIGIN      
        1 attgatgaga aattggtcaa agaagctgtt cgaaaagtag gcgatggtta tgtctttgag
       61 gagaatggag tttctcgtta tatcccagcc aaggatcttt cagcagaaac agcagcaggc
      121 attgatagca aactggccaa gcaggaaagt ttatctcata agctaggaac taagaaaact
      181 gacctcccat ctagtgatcg agaattttac aataaggctt atgacttact agcaagaatt
      241 caccaagatt tacttgataa taaaggtcga caagttgatt ttgaggcttt ggataacctg
      301 ttggaacgac tcaaggatgt ctcaagtgat aaagtcaagt tagtggaaga tattcttgcc
      361 ttcttagctc cgattcgtca tccagaacgt ttaggaaaac caaatgcgca aattacctac
      421 actgatgatg agattcaagt agccaagttg gcaggcaagt acacaacaga agacggttat
      481 atctttgatc ctcgtgatat aaccagtgat gagggggatg cctatgtaac tccacatatg
      541 acccatagcc actggattaa aaaagatagt ttgtctgaag ctgagagagc ggcagcccag
      601 gcttatgcta aagagaaagg tttgacccct ccttcgacag accatcagga ttcaggaaat
      661 actgaggcaa aaggagcaga agctatctac aaccgcgtga aagcagctaa gaaggtgcca
      721 cttgatcgta tgccttacaa tcttcaatat actgtagaag tcaaaaacgg tagtttaatc
      781 atacctcatt atgaccatta ccataacatc aaatttgagt ggtttgacga aggcctttat
      841 gaggcaccta aggggtatac tcttgaggat cttttggcga ctgtcaagta ctatgtcgaa
      901 catccaaacg aacgtccgca ttcagataat ggttttg
//
LOCUS       DAVHEW010000178          920 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVHEW010000178 DAVHEW010000000
VERSION     DAVHEW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.178"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..865)
                     /locus_tag="VVL53_002121"
     CDS             complement(<1..865)
                     /locus_tag="VVL53_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4184194.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEP
                     NEVNRDFPLYEA"
BASE COUNT          321 a          166 c          126 g          307 t
ORIGIN      
        1 ttgcttcata taacggaaaa tctctattca cctcatttgg ttcgatatca ttcattttat
       61 ttgactcaat tggattaatt gaatacaatt ttaaaaaaat aggcttcgta ccaataatag
      121 aggataattg acctcctaat tcacctagcg ctgtaaaaat aacacctgtt agtgctccta
      181 ttgcttcaat agtaccaatt ttcactattc cttttattgc aagatagcct gttaaaaaaa
      241 cgagagatat ctgaaaaaaa atattgagaa agaagctaat agcgcctgct aacgtttcta
      301 cagttgtctt tctttgtata accatcttta ataaaattcc tgcttcttta attttcttag
      361 gcaatacata taaaagattc aaggacgcta acacatcaaa tccattcaat atagtctcac
      421 tagattttaa aaaagcttca ttttggttag ttaaatttag actaacttct cgcattttcg
      481 atgcaaagat ttttggtaca agtagcataa tcattaatga aaacaaggtg gctacagtca
      541 atgaccaatg atagtgatta agagtcacaa ctgcaaatat agtaccagaa attcctttta
      601 ttactaaaaa aagttgttta aacgcctgat catttaaagt ctgaacatca ttatttagcc
      661 acgaaagata tgttcctgat gatttactat gaaattcttg ataggtagag ttagagatgt
      721 ctgtggcaac tctatttcga atctctagat taaactcttg gatcacttca acctgataat
      781 ttttcactac ccagtcaagg aatattatcc cacaccagac aatcatttgg tagattgaca
      841 atttcaaaaa ccgctctaaa ttcatcgcaa ttaattcatt caacaccaga gcattaatag
      901 ttgctgcata aattagcaat
//
LOCUS       DAVHEW010000179          917 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVHEW010000179 DAVHEW010000000
VERSION     DAVHEW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.179"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..>917)
                     /locus_tag="VVL53_002122"
     CDS             complement(<1..>917)
                     /locus_tag="VVL53_002122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184195.1"
                     /translation="EALVDAEADALVDADSEADVLAEADALVDAEAEALVDAEAEALV
                     DAEADALVLAEAEALVDAEAEALVDAEAEALVDAEADALVDAEAEALVDAEAEALVLA
                     EADALIDAEADALVDAEAEALVLAEADALVDADSEADVLAEADALIDAEADALVDAEA
                     EALVDADSDALVLAEAEALVDAEAEALVEAEALVLAEAEALVDAEAEALVLAEAEALV
                     LAEAEALVLAEAEALVDAEVDALVEAEADALVLAEADALVDADSDAEILAEAEALVDA
                     EAEALVLAEAEALVLAEAEALVDAEVDALV"
BASE COUNT          232 a          305 c          200 g          180 t
ORIGIN      
        1 acgagcgcat caacctcagc atcaaccagt gcctcagctt cagcaagtac tagtgcctca
       61 gcttcagcaa gtactagtgc ctcagcttca gcatcaacga gtgcctcagc ctcagcaagt
      121 atctcagcgt ctgaatcggc atcaacgagt gcgtcagcct cagcaagtac aagcgcgtca
      181 gcttcagcct caacgagcgc atcaacctca gcatcaacca gtgcctcagc ttcagcaagt
      241 actagtgcct cagcttcagc aagcaccagt gcctcagctt cagcaagcac aagcgcctca
      301 gcttccgcat caacaagcgc ctcagcctct gcaagtacca gtgcttcggc ctcaacaagc
      361 gcctcggctt cagcatcaac gagtgcttca gcttcagcaa gtaccagtgc atctgagtca
      421 gcatcaacaa gtgcctcagc ttcagcgtca accagtgcgt cagcctcagc atcaataagt
      481 gcgtcagctt cagcaagcac atcagcttct gaatctgcat caacaagtgc gtcagcctca
      541 gcaagtacaa gtgcctcagc ttcagcgtca accagtgcgt cagcctcagc atcaataagt
      601 gcgtcagctt cagcaagcac tagcgcctca gcctcagcat caaccagtgc ctcggcttca
      661 gcatcaacga gtgcgtcagc ctcagcatca acgagtgcct cggcttcagc atcaacgagt
      721 gcctcggctt cagcatcgac aagcgcctcg gcttcagcaa gtaccagtgc atcagcctca
      781 gcgtcaacga gtgcttcggc ttcagcatca acgagtgctt cagcttcagc atcaacgagt
      841 gcgtcggctt cagcaagcac atcagcttct gaatcagcat caacgagtgc gtcagcctca
      901 gcatcaacga gtgcctc
//
LOCUS       DAVHEW010000180          882 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVHEW010000180 DAVHEW010000000
VERSION     DAVHEW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.180"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(82..798)
                     /locus_tag="VVL53_002123"
     CDS             complement(82..798)
                     /locus_tag="VVL53_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4184196.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCNRDH"
BASE COUNT          258 a          140 c          179 g          305 t
ORIGIN      
        1 ccaaaaatag gtaagcgcta cctttttggt gtggtataat aagcatagaa aaagcccaag
       61 cgattagctc aggttttctt cttagtgatc acggttacat gagataaatt taatcttgta
      121 gtaatcagat cgtttgtaag tttcactgta ttctaaaact tggccagttg attcgagttt
      181 ggtgatttta gtttgtagga cagtagggaa ttgttcatcg actccgagga ctgaagccgc
      241 atgttctgga gttggaaaga ctatttcgtt gatttcttca aagtgttcat cattcatgtg
      301 aatgtggtag tctaacttga aacgattata gatagaacta tagtattcaa ggtttggata
      361 atttgcgttg atatattgtt ctgggatgta ggatgtatgg tagatataaa cgacaccgtt
      421 tgattcgcgg atacgttcaa tcttgtagta gaattgatcg ccacgtagac ccaatttttc
      481 caagtaaaca agcttgtttc cgcgttcaat tgaaagaaca gttaccttat catctttagc
      541 attgaagagt tcaatatctg aaaactctac aagcttgtgt ttgcgtgcac gtgaaacgaa
      601 ggttcctttt ccttgttggc ggacaatata gccatctttg gcaaggtcgt ttaaggcgcg
      661 aacaactgtg atagagctga catcgtacat tgaaatgagt tctgcttcag tgtaaaattt
      721 atctccactg ctaaactgcc cagagatgat tttatttttt aattcgtctt ttatgtattg
      781 atatttggga attgccatat ttttacctct attttccttc tccatagaaa attttaccat
      841 aaaagcgaaa aaaatagtag tcttttgaat aaaaaattag aa
//
LOCUS       DAVHEW010000181          873 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVHEW010000181 DAVHEW010000000
VERSION     DAVHEW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.181"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..610
                     /locus_tag="VVL53_002124"
     CDS             <1..610
                     /locus_tag="VVL53_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4184197.1"
                     /translation="FARNTTVKEFILNKDTGEVSELKPHRVTVTIQNGKEMSSTIVSE
                     EDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDTFIKPVFKKIEEKKEEE
                     NKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREEHSQKSDSTKDVTATVLDKNNISSK
                     STTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
BASE COUNT          357 a          101 c          166 g          249 t
ORIGIN      
        1 ttttgcaaga aatacaaccg taaaagaatt cattttaaat aaagatacgg gagaggtaag
       61 tgaattaaaa cctcataggg taactgtgac cattcaaaat ggaaaagaaa tgagttcaac
      121 gatagtgtcg gaagaagatt ttattttacc tgtttataag ggtgaattag aaaaaggata
      181 ccaatttgat ggttgggaaa tttctggttt cgaaggtaaa aaagacgctg gctatgttat
      241 taatctatca aaagatacct ttataaaacc tgtattcaag aaaatagagg agaaaaagga
      301 ggaagaaaat aaacctactt ttgatgtatc gaaaaagaaa gataacccac aagtaaacca
      361 tagtcaatta aatgaaagtc acagaaaaga ggatttacaa agagaagagc attcacaaaa
      421 atctgattca actaaggatg ttacagctac agttcttgat aaaaacaata tcagtagtaa
      481 atcaactact aacaatccta ataagttgcc aaaaactgga acagcaagcg gagcccagac
      541 actattagct gccggaataa tgtttatagt aggaattttt cttggattga agaaaaaaaa
      601 tcaagattaa gataaaagct atagaaaaaa atggtttatg tactgagatt agatagtgag
      661 gtgatgacat agttttgtga aaatagccat ttataactca attatttagt ttactttact
      721 ttactagtga tactatttgg agttattaat ggacttagtt tatataacta atgaattgat
      781 tgaaagggtt agtattgaca atattggtca tattgactag aaaatagagt ctatcaaaat
      841 ttaaaggcta atagaggtga tgagacaatt tcg
//
LOCUS       DAVHEW010000182          796 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVHEW010000182 DAVHEW010000000
VERSION     DAVHEW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.182"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(129..602)
                     /gene="tnpA"
                     /locus_tag="VVL53_002125"
     CDS             complement(129..602)
                     /gene="tnpA"
                     /locus_tag="VVL53_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEU4184198.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
BASE COUNT          221 a          177 c          142 g          256 t
ORIGIN      
        1 caaaaccacc agtgtgaact ggtggtttgt tctgcggcta taagccgctt ctaccggcca
       61 gggccaaagg cccaccgaaa tagcttcctc gcgcaccact ttcccgagca ggtgctaaag
      121 caccttagtt acttcctctt atttatttcg ccagtaaacg gatctactga ctcgaataac
      181 gtgagctggt ctgctactct gtcttcttgt aattgattct gaatatattc agctatcact
      241 ttctgattac ggcctaccgt atctacataa tagcctctac accaaaactt gcgattgcca
      301 tatttgtatt ttaaattcgc atgcttatca aaaatcatca aactgctctt gccctttaaa
      361 tagcccataa aggacgaaac actaagtttc ggaggaatac tgataagcat gtgaatatgg
      421 tctgaacaag cattcgcttc atggattatt acacccttac gctcacataa gtcacgtatg
      481 attcttccga tactagcttt gtatctgcca taaatgattt gacgacgata tttgggtgca
      541 aaaacaatat gatatttaca attccatgtg gtatgtgata aactttgatt atcctctctc
      601 atgaggtacc tcctgtatga tatgttgtag tggcggagaa accacttcta tcttatcatt
      661 ttaggaggtt ctttttgtta ccacgctaaa agctctatgg aaccactagc atagctagtg
      721 gttttcggga gacaacaaaa aagttggatg acccaacttt tatctttcta tcagtatcaa
      781 ttttatcttt ggtata
//
LOCUS       DAVHEW010000183          736 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVHEW010000183 DAVHEW010000000
VERSION     DAVHEW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.184"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            56..736
                     /locus_tag="VVL53_002126"
                     /pseudo
     CDS             56..736
                     /locus_tag="VVL53_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          224 a          133 c          158 g          221 t
ORIGIN      
        1 cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat ctactatatt
       61 tcgtctgatg ggcaaatctt ataaagagat tatagagctt ttagactgta atcaaacaac
      121 gatttggcga aacgtaaaaa aatatgagga gttcggactc gactctctcc ttcaagaaac
      181 acgtggtggt cgtaaccatg catatatgac agttgaggaa gagaaagcct ttcttgcccg
      241 ccatttgaag gctacagagg caggagaatt tgttacaatt gatgccttat ttcaggctta
      301 taaaaaggag ttaggtcgtt cctacacacg tgatgccttc tatcaactgt tgaagcgcca
      361 tggttggcga aatattacgc cacgtccaga acatcctaag aaagcagacg ctcaaaccat
      421 tgttgcgtct aaaaataaaa tctcaatcca agaaggcaag aaagcgtttt aaatatagta
      481 gacgttttcg taaggtttgc ttgatgtacc aagctgaagc tggtttcggt agaatcagta
      541 aactgggatc ttgttgggct ccaataggag taggtccaca tatccatagt cactatatac
      601 gagaatttcg ctattgttat ggagctgttg atgcctatac aggcgaatca tttttcttaa
      661 tagctggtag atgtaatact gagtggatga acgccttttt agaagagctt tcacaagctt
      721 atccttttac tcgtta
//
LOCUS       DAVHEW010000184          723 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVHEW010000184 DAVHEW010000000
VERSION     DAVHEW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.185"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            627..>723
                     /locus_tag="VVL53_002127"
     CDS             627..>723
                     /locus_tag="VVL53_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEU4184199.1"
                     /translation="MLAVLKTAYQLKHAKGGRKPKLSLEDLLMATL"
BASE COUNT          247 a          110 c          122 g          244 t
ORIGIN      
        1 agatgttttg atgaccattc atgatttgag taatccaagg tcaaaatgag tgctatacta
       61 gcagtgtaaa gattcttgtt caactaagat aaatcacaca tttaggagga aattttatga
      121 agaaaaaaat acttatcatt ttcatcttgt atctaatcat gtccatcttt ctttatccgc
      181 ttagggaaag tatttggtat cagctatttt ataccatagc ctatgtgatt gcagttatga
      241 tctattttgc tttaactaaa aagaaaggag caaagaaatg aaaacttttc ttgctaaaaa
      301 acggaacatc ttcctttcat ttggggattg aagtattata aaaagtagag cttaataaag
      361 tataaagggg taagtgtttt tgaaatactt acccttttat aatagattcc ctgcgaaaca
      421 aaatatggta tagtatagta gattgaaact agaatagtac gcctctactt ctaaaacatt
      481 gttagaaatc gatttgactg tcctgatcga tttatcctgt tcttatttca ttttactata
      541 gttctatgaa ttatgaagca agtaaataac taactgatgc acgatttaag cgtcttgttg
      601 gtgttcagcg cacgactttt gaagagatgt tagctgtatt aaaaacagct tatcaactta
      661 aacacgcaaa aggtggacga aaacctaaat taagcctaga agaccttctt atggccactc
      721 ttc
//
LOCUS       DAVHEW010000185          691 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVHEW010000185 DAVHEW010000000
VERSION     DAVHEW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.186"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..>691)
                     /locus_tag="VVL53_002128"
     CDS             complement(<1..>691)
                     /locus_tag="VVL53_002128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184200.1"
                     /translation="AEAEALVEAEADALVLAEAEALVLAEADALVDAEAEALVLAEAE
                     ALVLAEAEALVEADSDAEVLVLAEADALVDADSDADVLAEDEALVDAEAEALVLAEAE
                     ALVDAEADALVLAEADALVDAEAEAEALVLAEAEALVDAEADALVDAEADALVDAEAE
                     ALVDAEADALVLAEADALVDAEADALVDAEAEALVDADSDAEVLAEAEALVEAEAEAL
                     VDAEADALVLAE"
BASE COUNT          166 a          234 c          156 g          135 t
ORIGIN      
        1 cttcagcaag taccagtgca tcagcctcag cgtcaacgag tgcctcggct tccgcctcaa
       61 ccagtgcttc ggcttcagca agcacctcag cgtctgaatc ggcatcaaca agtgcctcag
      121 cttccgcatc aacgagtgcg tcggcctcag catcaacgag tgcatcagcc tcagcaagta
      181 ccagcgcatc agcctcagca tcaaccagtg cttcggcttc agcatcgaca agcgcatcag
      241 cttcagcgtc aacaagtgcg tcagcttccg catcaacgag tgcttcggct tcagcaagta
      301 caagtgcttc ggcctcagcc tcagcatcaa caagtgcgtc agcttcagca agcaccagtg
      361 cgtcggcttc agcatcgaca agcgcctcgg cttcagcaag taccagtgcc tcagcctcag
      421 catcaacaag tgcctcatcc tcagcaagca catcagcatc tgaatccgca tcaacaagtg
      481 cgtcagcctc agcaagtacc agtacctcag cgtctgaatc ggcctcaacg agtgcttcgg
      541 cttcagcaag tacaagtgct tcggcctcag caagtactag cgcctcagcc tcagcatcaa
      601 caagtgcgtc agcctcagca agcaccagtg cttcggcttc agcaagtacc agtgcgtcag
      661 cttcagcctc aacgagtgct tcggcttcag c
//
LOCUS       DAVHEW010000186          633 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVHEW010000186 DAVHEW010000000
VERSION     DAVHEW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.187"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            11..334
                     /gene="tnpB"
                     /locus_tag="VVL53_002129"
     CDS             11..334
                     /gene="tnpB"
                     /locus_tag="VVL53_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU4184201.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(489..633)
                     /locus_tag="VVL53_002130"
                     /pseudo
     CDS             complement(489..633)
                     /locus_tag="VVL53_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          177 a          124 c          115 g          217 t
ORIGIN      
        1 gtgttgctct atgacaattc atctatctag cctaggggag gtctatctcg tgtgtgggaa
       61 aactgatatg agacaaggaa tcgattcact ggcttatctg gttaaaaccc actttgaatt
      121 ggatcctttc tccggtcaag tctttctctt ttgtggtgga cgtaaagacc gctttaaagt
      181 cctttactgg gatggtcaag gattttggct actatataaa cgctttgaga acggcagatt
      241 gatttggcta agtacagaaa aggatgtcaa agctctcaca ccagaacaag tagactggct
      301 tatgaagggc ttttctatca ctccaaaaat atagtagatt gaaactagaa tagtacacct
      361 ctgcttctaa aacattgtta gaaatcgatt ttactgtcct gatcgatttg tcctgttctt
      421 atttcatttt actataaatc catcagaaag tcgtgatttc tattgaaatg aggactttct
      481 ttttatactc atctgctttc aaaaagcatt ctagtccatc tccgattaac gatggacttt
      541 atcacctcct tctccagtcc ttgtataaca tcttggagtt gattcatgac atcttccaaa
      601 gtttaaaagg ctttattctt aaatccacgt tta
//
LOCUS       DAVHEW010000187          605 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVHEW010000187 DAVHEW010000000
VERSION     DAVHEW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.188"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            243..311
                     /locus_tag="VVL53_002131"
                     /pseudo
     CDS             243..311
                     /locus_tag="VVL53_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            291..>605
                     /locus_tag="VVL53_002132"
     CDS             291..>605
                     /locus_tag="VVL53_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU4184202.1"
                     /translation="MMYLDGGFSIPRWPVATAINIAFNGVLGGGAISLVRNYIRNYGL
                     RRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGNAITTIWANNDVNRRDP
                     NLN"
BASE COUNT          208 a           79 c          130 g          188 t
ORIGIN      
        1 cgttattaag tcaaaaaaaa taaattttga aatagtatta gtgagttcgc aatcatttat
       61 tgctagtggt ttagaactgt taagtttcaa acaataattg attttaaacc ttaggtaacc
      121 tataaaaaca aataaggtag agaaaggata tcttatctag gtagctaagt gataaaaaca
      181 cagtcgtcta tcacttgtta ggaaactatc ctaactatgc ttaataaaaa aggagaaaat
      241 aaatggaact cgtattacca aataattatg ttgttcttga gcaagaagag atgatgtatc
      301 ttgatggggg attttctatt ccgagatggc ctgttgcaac agccattaat atagctttta
      361 atggtgtttt aggtggagga gcaatcagtc tagttagaaa ttatattcgt aattatggtt
      421 tgcggcgagt tacaagcgca attgctggag cagctgcaag atatgttggg gtacgagttg
      481 caaatagagt ggcaggattt gcactgtctg ctattaatgg atttgcagct tggatgtcaa
      541 ttggcaatgc tattacaaca atctgggcca ataatgatgt aaataggaga gacccaaatt
      601 taaac
//
LOCUS       DAVHEW010000188          565 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVHEW010000188 DAVHEW010000000
VERSION     DAVHEW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.189"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            112..294
                     /locus_tag="VVL53_002133"
     CDS             112..294
                     /locus_tag="VVL53_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4184203.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            239..>565
                     /locus_tag="VVL53_002134"
     CDS             239..>565
                     /locus_tag="VVL53_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184204.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTAYQLKHAKGGRKPKLSLEYRTYEEIAADFGIHESNLIRRSQWVEVTLVQSGVT
                     ISRTPLS"
BASE COUNT          219 a           98 c          103 g          145 t
ORIGIN      
        1 tgggttaaaa aagaaattaa ctgagtaaaa actctcgaaa ttcaattcgc tatattatca
       61 ctcaacttga acaaatacca gaaccacaaa aaacagatgt aaaggaaaaa tatggactta
      121 aaatttgaag gggttgattt agaatacaaa aaagctaaaa acaatcttcc agagtctttc
      181 tgggaaacct actcagcatt tgcaaataca aatggcggga aaataattct cgggatagat
      241 gaaaaaaaca ttgacaccta ccaaagagtc aatagactcc ctgcgaaatt atgaagcaag
      301 taaacaatta actgatacac gatttaagcg tcttgttggt gttcagcgca cgacttttaa
      361 agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa
      421 acctaaatta agcctagaat accgcactta tgaagaaatt gcggctgatt ttggtattca
      481 cgaaagcaac ttaatccgtc ggagccaatg ggttgaagta actcttgttc aaagtggtgt
      541 tacgatttca agaactcctc ttagt
//
LOCUS       DAVHEW010000189          562 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVHEW010000189 DAVHEW010000000
VERSION     DAVHEW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.190"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            342..>562
                     /locus_tag="VVL53_002135"
     CDS             342..>562
                     /locus_tag="VVL53_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU4184205.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFD"
BASE COUNT          183 a           75 c          115 g          189 t
ORIGIN      
        1 tagagattac aaggcttgtt taagaaagaa ttcaaagacc ttgacaaata aaaataaaat
       61 ggttattata aaaaatggtc tgaaatagat gatgatactt ttcgaaaatc tttttacgtc
      121 agctcagctt tgccttgctg tgttttgagc aagctacggt tagcttccga gtttgatttt
      181 catttactag aaatgaaact gatgagagat atcagtagac atttgagtca ggatattatg
      241 gaaaatgata aaaagagctc gtgagattgg catatcagac tactaaagta ttgagtttgt
      301 taggatttta gcgactagtt agctgggaaa ggaagatatt tgtgacaaat aataaactgt
      361 attcgttgat agaatttaga aataaaatat atgaagaatt agaactttcc agaagtgatt
      421 tagcgatttt actatgtgcc atgcttatcg cctctatcgg attaaatatg gattcgactc
      481 ccgtgattat tggagccatg ttaatctctc ctttgatgac acctattctg ggagtggggc
      541 tctctctagc tatatttgat tt
//
LOCUS       DAVHEW010000190          547 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVHEW010000190 DAVHEW010000000
VERSION     DAVHEW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.191"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..404)
                     /locus_tag="VVL53_002136"
     CDS             complement(<1..404)
                     /locus_tag="VVL53_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184206.1"
                     /translation="MEIPWEVILNEFKQAIGTDLLDNTAQYVEKLIEFLISFKHLQVE
                     DLLRNYIVRSTRSILDSIAYEAQETADLRVSNGETITLDDFNKILLNAITNFSLEISK
                     VEAESNFEFFEAELELIKSIVDDVFKSFPHTD"
BASE COUNT          185 a          101 c           69 g          192 t
ORIGIN      
        1 tcatcagtat gaggaaagga tttaaaaaca tcgtctacaa tgctttttat taactctaac
       61 tcagcttcaa aaaattcaaa attactttca gcttctactt ttgaaatttc taaactaaaa
      121 ttagttatag catttaataa aattttatta aaatcatcta gagtgatggt ttcaccatta
      181 gaaactctta aatcagctgt ttcttgcgct tcataggcaa tgctgtccaa aatacttctt
      241 gtacttctga caatataatt tcttaataaa tcctcaactt gtagatgttt aaaggaaatt
      301 aaaaattcta ttagcttttc aacgtattgg gcagtattat ctaataaatc tgtgccaata
      361 gcctgcttaa actcatttaa aattacctcc cacggaattt ccataaacga agcgttccca
      421 tatatcatga tccccacgga atgttctttt gataaagtga ataattttcg ggcgctatta
      481 aaaacttttg aatttttccc gtctgataag gttacagcgc tatcagaagc caatacaaca
      541 ccatttt
//
LOCUS       DAVHEW010000191          514 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0328-P
            SAMN37284158-rid19589583.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVHEW010000191 DAVHEW010000000
VERSION     DAVHEW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284158
            Sequence Read Archive: SRR25917637
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913177.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913177.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:08:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,090
            Genes (coding)                    :: 1,969
            CDSs (with protein)               :: 1,969
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 31 of 121
            Pseudo Genes (multiple problems)  :: 26 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284158-rid19589583.denovo.192"
                     /serotype="19A"
                     /isolate="SC22-0328-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(4..285)
                     /locus_tag="VVL53_002137"
     CDS             complement(4..285)
                     /locus_tag="VVL53_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4184207.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKNQQGNTMESYF"
BASE COUNT          154 a          104 c           58 g          198 t
ORIGIN      
        1 taactaaaag taagattcca ttgtatttcc ttgttggttt ttcatatcac gaattttatt
       61 tttcagaata ttaaagttac aaatttcatc tctaatatcg ttatccgctc tgtagccatt
      121 aatcatctca gtcagagttt tccctttact accattcttt atagtagtat ttactctttt
      181 tatactgctt tcaaaaggct catatgtttt atacttaatt acttcgtctt taaaattaaa
      241 taaaggtgta tccggcttag cattccataa aataatcaac cacattatag cattataaag
      301 ctgctttgca gtttcattcc acttttcagg ttataaattt ccactccctg tattgtcata
      361 ttcttgtcta ttatttacat gagaatcacc tacaagcgga ttaaaaagag gtagcccttt
      421 gtgattaatt agttctaaaa aatcccttgc cgcacccatg caacactgaa taacttcctt
      481 cttgccatca attttactct cagcgataaa taca
//
